title: CRC Patient 140


title: CRC Patient 157

status: Public on Nov 28 2016

type: RNA

city: Parkville

first: Oliver

last: Sieber

state: VIC

address: 1G Royal Parade

country: Australia

institute: Walter and Eliza Hall Insitute of Medical Reseach

hyb_protocol: Standard Affymetrix procedures

scan_protocol: Standard Affymetrix procedures

data_processing: RMA-sketch normalization on the core probeset using Affymetrix Power Tools (APT) followed by batch correction using the ComBat algorithm.

label: biotin

molecule: total RNA

organism: Homo sapiens

source_name: primary colon adenocarcinoma

label_protocol: Approximately 8 ug of total RNA was processed to produce biotinylated cRNA targets.

extract_protocol: Surgically isolated proximal colon cancer specimens were immediately frozen in liquid nitrogen. Total RNA was extracted from cancer tissue using the AllPrep DNA/RNA Micro Kit (Qiagen).

tag: patient id

tag: analysis batch

tag: other geo code

tag: age at diagnosis

tag: gender

value: female

tag: tumor stage

value: Tumor stage III

tag: primary site

value: left

tag: microsatellite status

value: MSS

tag: braf v600e status

value: wt

tag: kras codon 12, 13, 61 status


title: CRC Patient 139

value: male

value: right

value: MSI-H

value: mut


title: CRC Patient 134


title: CRC Patient 141


title: CRC Patient 144


title: CRC Patient 132


title: CRC Patient 131


title: CRC Patient 136


title: CRC Patient 135


title: CRC Patient 128


title: CRC Patient 133


title: CRC Patient 130

value: Tumor stage IV


title: CRC Patient 137


title: CRC Patient 129


title: CRC Patient 127


title: CRC Patient 125


title: CRC Patient 124


title: CRC Patient 126


title: CRC Patient 120


title: CRC Patient 117


title: CRC Patient 112


title: CRC Patient 115


title: CRC Patient 116


title: CRC Patient 119


title: CRC Patient 110


title: CRC Patient 114


title: CRC Patient 118


title: CRC Patient 111


title: CRC Patient 107


title: CRC Patient 106


title: CRC Patient 105


title: CRC Patient 102


title: CRC Patient 104


title: CRC Patient 99


title: CRC Patient 113


title: CRC Patient 97


title: CRC Patient 100


title: CRC Patient 122


title: CRC Patient 101


title: CRC Patient 98


title: CRC Patient 93


title: CRC Patient 96


title: CRC Patient 108


title: CRC Patient 95


title: CRC Patient 94


title: CRC Patient 103


title: CRC Patient 91


title: CRC Patient 92


title: CRC Patient 87


title: CRC Patient 90


title: CRC Patient 86


title: CRC Patient 89


title: CRC Patient 84


title: CRC Patient 85


title: CRC Patient 83


title: CRC Patient 67


title: CRC Patient 68


title: CRC Patient 74


title: CRC Patient 65


title: CRC Patient 61


title: CRC Patient 44


title: CRC Patient 51


title: CRC Patient 43


title: CRC Patient 63


title: CRC Patient 55


title: CRC Patient 46


title: CRC Patient 40


title: CRC Patient 41


title: CRC Patient 38


title: CRC Patient 66


title: CRC Patient 36


title: CRC Patient 35


title: CRC Patient 33


title: CRC Patient 34


title: CRC Patient 32


title: CRC Patient 25


title: CRC Patient 23


title: CRC Patient 39


title: CRC Patient 24


title: CRC Patient 29


title: CRC Patient 26


title: CRC Patient 27


title: CRC Patient 28


title: CRC Patient 22


title: CRC Patient 21


title: CRC Patient 15


title: CRC Patient 31


title: CRC Patient 12


title: CRC Patient 17


title: CRC Patient 11


title: CRC Patient 18


title: CRC Patient 16


title: CRC Patient 7


title: CRC Patient 8


title: CRC Patient 9


title: CRC Patient 37


title: CRC Patient 5


title: CRC Patient 153


title: CRC Patient 3


title: CRC Patient 2


title: CRC Patient 1


title: CRC Patient 6


title: CRC Patient 10


title: CRC Patient 258

data_processing: RMA  normalization.

extract_protocol: Surgically isolated colorectal cancers were immediately frozen in liquid nitrogen. Total RNA was extracted from cancer tissue using Trizol reagent (Invitrogen).

tag: other geo code (gse14333)

tag: other geo code (gse13067)


title: CRC Patient 248

value: Tumor stage II


title: CRC Patient 4


title: CRC Patient 251


title: CRC Patient 250


title: CRC Patient 177


title: CRC Patient 249


title: CRC Patient 88


title: CRC Patient 176


title: CRC Patient 82


title: CRC Patient 247


title: CRC Patient 187


title: CRC Patient 78


title: CRC Patient 81


title: CRC Patient 20


title: CRC Patient 87


title: CRC Patient 175


title: CRC Patient 167


title: CRC Patient 79


title: CRC Patient 166


title: CRC Patient 77


title: CRC Patient 73


title: CRC Patient 252


title: CRC Patient 80


title: CRC Patient 71


title: CRC Patient 69


title: CRC Patient 64


title: CRC Patient 76

value: Tumor stage I


title: CRC Patient 67


title: CRC Patient 174


title: CRC Patient 61


title: CRC Patient 84


title: CRC Patient 72


title: CRC Patient 57


title: CRC Patient 59


title: CRC Patient 58


title: CRC Patient 56


title: CRC Patient 75


title: CRC Patient 123


title: CRC Patient 53


title: CRC Patient 49


title: CRC Patient 51


title: CRC Patient 70


title: CRC Patient 47


title: CRC Patient 46


title: CRC Patient 62


title: CRC Patient 44


title: CRC Patient 42


title: CRC Patient 48


title: CRC Patient 43


title: CRC Patient 30


title: CRC Patient 19


title: CRC Patient 14


title: CRC Patient 45


title: CRC Patient 52


title: CRC Patient 55


title: CRC Patient 50


title: CRC Patient 13


title: CRC Patient 11


title: pfkB1 mutant cultivated with fructose at 12 h

city: Kizugawa

first: Satoshi

last: Hasegawa

state: Kyoto

address: 9-2, Kizugawadai

department: molecular microbiology and biotechnology group

country: Japan

institute: Research Institute of Innovative Technology for the Earth

hyb_protocol: Hybridization was performed using Gene Expression Hybridization Kit according to the manufacture’s manual (Agilent). The labeled cDNA was hybridized to microarrays in an Agilent Technologies Microarray chamber at 65˚C for 17 h in a rotating Agilent hybridization oven. After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent), and 1 minute with 37˚C GE Wash buffer 2 (Agilent).

scan_protocol: Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505C) at a resolution of 5 µm using single color scan setting for 4x44k array slides. PMT is set to 100% for Cy3 channels.

data_processing: The scanned images were quantified with Feature Extraction Software 10.5.5.1 (Agilent) using default parameters (protocol GE1_105_Dec08). Feature extracted data were analyzed using GeneSpring GX v 12.5 software from Agilent. Normalization of the data was done in GeneSpring GX using the recommended Percentile shift normalization (75th percentile) with baseline to median of wt(Glc)_4h as a control sample.

organism: Corynebacterium glutamicum R

source_name: The pfkB1 mutant strain cultivated with fructose at 12 h

label_protocol: The cDNAs were synthesized from RNA by using reverse transcriptase from 10 μg of total RNAs and labeled with cyanine 3 (Cy3) using the SuperScript Indirect cDNA Labeling system (Life Technologies).

growth_protocol: C. glutamicum strains are grown in 100 ml of nutrient rich A medium containing 4% glucose or fructose in 500 ml flask at 33˚C

extract_protocol: Total RNA was extracted from C. glutamicum cells using the Qiagen RNeasy Mini kit (Qiagen) according to the manufacture's manual.

tag: genotype

tag: protocol

value: cultivated with fructose

tag: time


title: pfkB1 mutant cultivated with glucose at 12 h

source_name: The pfkB1 mutant strain cultivated with glucose at 12 h

value: cultivated with glucose


title: pfkB1 mutant cultivated with glucose at 4 h

source_name: The pfkB1 mutant strain cultivated with glucose at 4 h


title: wt cultivated with fructose at 12 h

source_name: The wild type strain cultivated with fructose at 12 h

value: wild type


title: CRC Patient 12


title: pfkB1 mutant cultivated with fructose at 4 h

source_name: The pfkB1 mutant strain cultivated with fructose at 4 h


title: wt cultivated with glucose at 4 h

source_name: The wild type strain cultivated with glucose at 4 h


title: SI_01

city: Shizuoka City

first: Nobuyoshi

last: Shiojiri

address: Oya 836, Suruga-ku

department: Department of Biology

institute: Shizuoka University

hyb_protocol: 600 ng of Cy3-labelled cRNA was fragmented at 60°C for 30 minutes in a reaction volume of 25 µl containing 1x Agilent Fragmentation Buffer and 2x Agilent Blocking Agent following the manufacturer’s instructions. On completion of the fragmentation reaction, 25 µl of 2x Agilent GE hybridization buffer HI-RPM was added to the fragmentation mixture and hybridized to Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray for 17 hours at 65°C in a rotating Agilent hybridization oven. After hybridization, microarrays were washed 1 minute at room temperature with Agilent Gene Expression Wash Buffer 1 and 1 minute with 37°C Agilent Gene Expression Wash Buffer 2, then dried.

scan_protocol: Slides were scanned immediately after washing on the Agilent G2565A Microarray Scanner System using one color scan setting for 8x60k array slides (Scan Area 61x21.6 mm, Scan resolution 3 µm, Dye channel is set to Green and Green PMT is set to 100%).

data_processing: The scanned images were quantified using Agilent’s Feature Extraction Software version 9.5. Quantified probe signals were normalized by Subio platform version 1.16 with Basic Plug-in (Subio).

organism: Mus musculus

source_name: E12.5 mouse  small intestine

label_protocol: Cyanine-3 (Cy3) labeled cRNA was prepared from 50 ng total RNA using the Quick Amp Labeling Kit (Agilent) according to the manufacturer’s instructions, followed by purification with RNeasy Mini Kit (Qiagen). Dye incorporation and cRNA yield were checked with the BioSpec-nano spectrophotometer (Shimadzu).

extract_protocol: Total RNA was extracted from the VYS, PYS (endodermal cells and Reichert’s membrane), placenta, liver and small intestine at E12.5 with RNeasy Mini kit (Qiagen, Hilden, Germany) in accordance with the manufacturer’s instructions. Quality assessment of the total RNA was performed using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).

tag: tissue

value: Small intestine

tag: Stage


title: Liv_02

source_name: E12.5 mouse  liver

value: Liver


title: wt cultivated with glucose at 12 h

source_name: The wild type strain cultivated with glucose at 12 h


title: Pla_02

source_name: E12.5 mouse  placenta

value: Placenta


title: Pla_01


title: Liv_01


title: PYS_02

source_name: E12.5 mouse  parietal yolk sac

value: Parietal yolk sac


title: PYS_03


title: wt cultivated with fructose at 4 h

source_name: The wild type strain cultivated with fructose at 4 h


title: PYS_01


title: VYS_03

source_name: E12.5 mouse  visceral yolk sac

value: Visceral yolk sac


title: VYS_01


title: VYS_02


title: Cd1d wild type 3 (Cd1d +/+)

status: Public on Nov 27 2016

city: Lausanne

first: Angus

last: Moodycliffe

country: Switzerland

institute: Nestlé Reseach Center

description:
Whole skin tissue was harvested from 5 individual non UVB-irradiated inbred (C57BL/6 background) CD1d knockout (CD1d-/-) and 4 individual (C57BL/6) wild-type (CD1d+/+) mice, age and sex matched. Ten micrograms of RNA extracted from each skin tissue sample represented the starting material for all individual mouse samples. Study samples were judged to contain sufficient amounts of high-quality RNA for hybridization to Affymetrix MG-U74 GeneChips (Affymetrix, Inc., Santa Clara, CA). In general, total RNA was converted to biotinylated cRNA, hybridized in the Affymetrix probe array cartridge, stained, and then quantified. First and second strand cDNA synthesis was performed using the SuperScript Choice System (Invitrogen AG, Basel, Switzerland), according to manufacturer instructions, but using an oligo-dT primer containing a T7 RNA polymerase binding site. Labeled cRNA was prepared with the RNA Transcript Labeling kit (Enzo Biochem Inc., NY). Biotinylated CTP and UTP were used together with unlabeled NTPs in the reaction, and unincorporated nucleotides were removed with Nucleospin columns (Macherey-Nagel, Düren, Germany ). cRNA (20 µg) was fragmented at 94 °C for 35 min in buffer containing 200 mM Tris-acetate pH 8.1, 500mM KOAc, 150 mM MgOAc. Prior to hybridization, fragmented cRNA in hybridization mix ( Buffer containing 100 mM MES, 1M NaCl, 20 mM EDTA, 0.01 % Tween 20, 0.5 ng/ml BSA, 0.1 ng/ml herring sperm and Affymetrix controls ), was heated to 95 °C for 5 min, cooled to 45 °C and loaded onto an Affymetrix probe array cartridge. The probe array was incubated for 16 h at 45 °C at constant rotation (60 rpm), then exposed to Affymetrix washing and staining protocol. Prior to hybridisation, Affymetrix test chips (Test chip 5' / 3' ratios were less than 3.0) were used to confirm that all samples give strong signals for pre-selected genes. Data processing was performed using the commercially available program Microarray Suite, version 5.0 (MAS5), (Affymetrix, Inc., Santa Clara, CA).
Keywords = CD1d knockout, UV, sunburn, cancer, inflammation, cell survival

source_name: skin Cd1d wild type 3 (Cd1d +/+)


title: Cd1d wild type 4 (Cd1d +/+)

source_name: skin Cd1d wild type 4 (Cd1d +/+)


title: Cd1d wild type 2 (Cd1d +/+)

source_name: skin Cd1d wild type 2 (Cd1d +/+)


title: CRC Patient 60

source_name: primary colon adenocarcinoma

tag: patient id

value: MSS


title: CRC Patient 54

value: left

value: wt


title: JJN3_ANTI-GAL1_REOX

city: MILAN

first: Luca

last: Agnelli

address: Venezian 1

department: Department of Advanced Diagnostics

country: Italy

institute: IRCCS Istituto Nazionale dei Tumori

description: Gene expression profiling of JJN3 infected with anti-Gal-1 vector after re-oxygenation of 48h

hyb_protocol: Following fragmentation, 15 micrograms of cRNA were hybridized for 16 hr  at 45°C on GeneChip HG-U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.

scan_protocol: HG-U133 2.0 Plus arrays were scanned using the Scanner G3000 7G (Affymetrix).

data_processing: Normalized expression values were obtained by the RMA algorithm and reannotation of HG-U133 Plus 2.0 arrays was performed using the corresponding GeneAnnot custom chip deﬁnition ﬁles (GA v2.2.0).

label_protocol: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 nanograms of total RNA (Expression Analysis Technical Manual, Affymetrix).

growth_protocol: JJN3 were maintained in culture in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS), Penicillin (100U/ml), Streptomycin (100μg/ml), L-Glutamine (2mM) and Fungizone

extract_protocol: Total RNA was extracted from JJN3 cells with Rneasy Mini Kit (Qiagen, Valencia, CA)

treatment_protocol: Normoxic condition was maintained at 20% of O2 and 5% of CO2 and hypoxic treatment was obtained by exposing JJN3 and OPM2 at 1% O2, 94% N2 and 5% CO2 for 48 hours. The effect of re-oxygenation was also tested by a subsequently incubation for 48 hours at 20% of O2 and 5% of CO2.

tag: cell line

tag: cell type

value: human myeloma cell line


title: Cd1d knock out 4 (Cd1d -/-)

source_name: skin Cd1d knock out 4 (Cd1d -/-)


title: Cd1d knock out 5 (Cd1d -/-)

source_name: skin Cd1d knock out 5 (Cd1d -/-)


title: Cd1d wild type 1 (Cd1d +/+)

source_name: skin Cd1d wild type 1 (Cd1d +/+)


title: JJN3_ANTI-GAL1_HYPO

description: Gene expression profiling of JJN3 infected with anti-Gal-1 vector incubated in hypoxia for 48h


title: Cd1d knock out 2 (Cd1d -/-)

source_name: skin Cd1d knock out 2 (Cd1d -/-)


title: Cd1d knock out 3 (Cd1d -/-)

source_name: skin Cd1d knock out 3 (Cd1d -/-)


title: JJN3_ANTI-GAL1_NORM

description: Gene expression profiling of JJN3 infected with anti-Gal-1 vector incubated in normoxia for 48h


title: JJN3_SCR_REOX

description: Gene expression profiling of JJN3 infected with scamble vector after re-oxygenation of 48h


title: RDES

type: genomic

city: L'Hospitalet del Llobregat

first: Sebastian

last: Moran

state: Barcelona

address: Av. Gran Via de l'Hospitalet, 199-203

department: PEBC

country: Spain

institute: IDIBELL

hyb_protocol: Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol

scan_protocol: Arrays were imaged using High Scan SQ using standard recommended Illumina scanner setting

data_processing: Genome Studio V2011.2

label: Cy3, Cy5

molecule: genomic DNA

source_name: Cell Line (Ewing's sarcoma)

label_protocol: Standard Illumina labelling protocol

extract_protocol: Total DNA was isolated by standard procedures

value: RDES


title: Cd1d knock out 1 (Cd1d -/-)

source_name: skin Cd1d knock out 1 (Cd1d -/-)


title: JJN3_SCR_NORM

description: Gene expression profiling of JJN3 infected with scamble vector incubated in normoxia for 48h


title: JJN3_SCR_HYPO

description: Gene expression profiling of JJN3 infected with scamble vector incubated in hypoxia for 48h


title: WT vs VgDome midgut 1

status: Public on Nov 26 2016

city: Baltimore

first: George

last: Dimopoulos

state: Maryland

address: 615 N Wolfe St.

department: Molecular Microbiology and Immunology

country: USA

institute: Johns Hopkins School of Public Health

hyb_protocol: Probes prepared from the RNA of transgenic mosquitoes were co-hybridized with probes prepared from the RNA obtained from wild-type mosquitoes.  The hybridizations were conducted on an Agilent-based microarray platform using custom-designed whole-genome 8 x 60K A. aegypti microarrays

scan_protocol: The arrays were scanned with an Agilent High Resolution Scanner (Agilent Technologies Inc., Santa Clara, CA, USA).

data_processing: Spot intensity was quantified using Agilent Feature Extraction Software. Background-subtracted median fluorescent values were normalized with the LOWESS normalization method, and Cy5/Cy3 ratios from replicate assays were subjected to t-tests at a significance level of p<0.05 using TIGR MIDAS and MeV software. Expression data from replicate assays were averaged with the GEPAS microarray software and logarithm (base 2)-transformed.

organism: Aedes aegypti

source_name: Midgut 24 hr after bloodmeal

label_protocol: Cy5- and Cy3-labeled probes were synthesized from 200 ng of total RNA of each sample using Low Input Quick Amp Labeling Kit from Agilent Technologies

growth_protocol: Mosquitoes were reared at 27°C in 70% humidity, and adults were maintained on a 10% sucrose solution on a 12-h light/dark cycle, according to standard rearing conditions.

extract_protocol: Pools of at least 10 midguts or fat bodies were collected from each treatment type. RNA was extracted from dissected tissues using an RNeasy kit (Qiagen) according to standard protocol.

treatment_protocol: Mosquitoes were provided a blood meal. 24 hours after feeding, mosquitoes were aneshtetized on ice and midguts or fat bodies were collected.

tag: genotype/variation

value: WT (ORLxRock)

value: Midgut

value: VgDome


title: A673


title: TC71


title: WT vs VgHop midgut 4

value: VgHop


title: 349T1b

source_name: Primary tumor (Ewing's sarcoma)

value: Primary tumor (Ewing's sarcoma)


title: CADOES1


title: WT vs VgDome midgut 3


title: WT vs VgHop midgut 3


title: TC252


title: EW7


title: 336T1b


title: WT vs VgDome midgut 4


title: WT vs VgHop midgut 2


title: WT vs VgHop midgut 1


title: WT vs VgDome Fat body 4

source_name: Fat body 24 hr after bloodmeal

value: Fat body


title: WT vs VgDome midgut 2


title: 308T1b


title: 192T1c


title: STAET1


title: 124T


title: 232T1a


title: WT vs VgDome Fat body 2


title: WT vs VgDome Fat body 1


title: 441T1b


title: T117


title: 114Th


title: 117T1d


title: 263T1d


title: T208


title: T63


title: 432T1


title: WT vs VgHop Fat body 2


title: 145T


title: WT vs VgHop Fat body 3


title: T0427rep3

city: Hitachi

first: Kentaro

last: Mishima

state: Ibaraki

address: 3809-1 Juochoishi

department: Breeding Department

institute: Forest Tree Breeding Center

description: This sample is of C.japonica plus-tree (Tano1) in 27 April. It is the fourth of four biological replicates and third of three Technical replicatesused in this experiment.

hyb_protocol: Hybridization was performed by NimbleGen Systems Inc., Madison, WI, USA following their standard operating protocol. See www.nimblegen.com.

scan_protocol: Scanning was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.

data_processing: The raw data (.pair file) was background correction as implemented in the NimbleScan software package, version 2.6 (Roche NimbleGen, Inc.).

organism: Cryptomeria japonica

label_protocol: Labeling was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.

growth_protocol: Sample was taken from 15-year-old trees of C.japonica plus-tree in Hitachi, Ibaraki Prefecture.

extract_protocol: Total RNA was isolated from tissue of the cambium region and differentiating xylem of plus-trees using an RNeasy Plant Mini kit (Qiagen, Gaithersburg, MD, USA). The quality of total RNA was assessed by measuring the ratio of absorption at 260 nm and 280 nm via an Agilent Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA, USA).

treatment_protocol: To evaluate how gene expression and morphological development in the cambial region changed over a single growing season, tissues from this region were collected from different trees at the same time (around 10 AM) on 15 different dates from 2010 to 2011.

value: cambium region and differentiation xylem

tag: cultivar

tag: sampling date


title: WT vs VgHop Fat body 1


title: T0824rep2

description: This sample is of C.japonica plus-tree (Tano1) in 24 August. It is the fourth of four biological replicates and second of three Technical replicatesused in this experiment.


title: WT vs VgDome Fat body 3


title: T0427rep1

description: This sample is of C.japonica plus-tree (Tano1) in 27 April. It is the fourth of four biological replicates and first of three Technical replicatesused in this experiment.


title: N1007rep3

description: This sample is of C.japonica plus-tree (Nishikawa10) in 7 October. It is the third of four biological replicates and third of three Technical replicatesused in this experiment.


title: T0622rep1

description: This sample is of C.japonica plus-tree (Tano1) in 22 June. It is the fourth of four biological replicates and first of three Technical replicatesused in this experiment.


title: T1007rep2

description: This sample is of C.japonica plus-tree (Tano1) in 7 October. It is the fourth of four biological replicates and second of three Technical replicatesused in this experiment.


title: T0824rep3

description: This sample is of C.japonica plus-tree (Tano1) in 24 August. It is the fourth of four biological replicates and third of three Technical replicatesused in this experiment.


title: T0622rep2

description: This sample is of C.japonica plus-tree (Tano1) in 22 June. It is the fourth of four biological replicates and second of three Technical replicatesused in this experiment.


title: T1007rep3

description: This sample is of C.japonica plus-tree (Tano1) in 7 October. It is the fourth of four biological replicates and third of three Technical replicatesused in this experiment.


title: WT vs VgHop Fat body 4


title: T1007rep1

description: This sample is of C.japonica plus-tree (Tano1) in 7 October. It is the fourth of four biological replicates and first of three Technical replicatesused in this experiment.


title: N0824rep1

description: This sample is of C.japonica plus-tree (Nishikawa10) in 24 August. It is the third of four biological replicates and first of three Technical replicatesused in this experiment.


title: N1007rep2

description: This sample is of C.japonica plus-tree (Nishikawa10) in 7 October. It is the third of four biological replicates and second of three Technical replicatesused in this experiment.


title: T0324rep3

description: This sample is of C.japonica plus-tree (Tano1) in 24 March. It is the fourth of four biological replicates and Third of three Technical replicatesused in this experiment.


title: N0622rep1

description: This sample is of C.japonica plus-tree (Nishikawa10) in 22 June. It is the third of four biological replicates and first of three Technical replicatesused in this experiment.


title: N0824rep2

description: This sample is of C.japonica plus-tree (Nishikawa10) in 24 August. It is the third of four biological replicates and second of three Technical replicatesused in this experiment.


title: T0824rep1

description: This sample is of C.japonica plus-tree (Tano1) in 24 August. It is the fourth of four biological replicates and first of three Technical replicatesused in this experiment.


title: N1007rep1

description: This sample is of C.japonica plus-tree (Nishikawa10) in 7 October. It is the third of four biological replicates and first of three Technical replicatesused in this experiment.


title: T0622rep3

description: This sample is of C.japonica plus-tree (Tano1) in 22 June. It is the fourth of four biological replicates and third of three Technical replicatesused in this experiment.


title: N0622rep2

description: This sample is of C.japonica plus-tree (Nishikawa10) in 22 June. It is the third of four biological replicates and second of three Technical replicatesused in this experiment.


title: T0324rep1

description: This sample is of C.japonica plus-tree (Tano1) in 24 March. It is the fourth of four biological replicates and first of three Technical replicatesused in this experiment.


title: N0824rep3

description: This sample is of C.japonica plus-tree (Nishikawa10) in 24 August. It is the third of four biological replicates and third of three Technical replicatesused in this experiment.


title: N0622rep3

description: This sample is of C.japonica plus-tree (Nishikawa10) in 22 June. It is the third of four biological replicates and third of three Technical replicatesused in this experiment.


title: T0427rep2

description: This sample is of C.japonica plus-tree (Tano1) in 27 April. It is the fourth of four biological replicates and second of three Technical replicatesused in this experiment.


title: N0427rep3

description: This sample is of C.japonica plus-tree (Nishikawa10) in 27 April. It is the third of four biological replicates and third of three Technical replicatesused in this experiment.


title: T0324rep2

description: This sample is of C.japonica plus-tree (Tano1) in 24 March. It is the fourth of four biological replicates and second of three Technical replicatesused in this experiment.


title: N0427rep2

description: This sample is of C.japonica plus-tree (Nishikawa10) in 27 April. It is the third of four biological replicates and second of three Technical replicatesused in this experiment.


title: N0427rep1

description: This sample is of C.japonica plus-tree (Nishikawa10) in 27 April. It is the third of four biological replicates and first of three Technical replicatesused in this experiment.


title: N0324rep1

description: This sample is of C.japonica plus-tree (Nishikawa10) in 24 March. It is the third of four biological replicates and first of three Technical replicatesused in this experiment.


title: I1007rep1

description: This sample is of C.japonica plus-tree (Iiyama9) in 7 October. It is the second of four biological replicates and first of three Technical replicatesused in this experiment.


title: N0324rep3

description: This sample is of C.japonica plus-tree (Nishikawa10) in 24 March. It is the third of four biological replicates and Third of three Technical replicatesused in this experiment.


title: N0324rep2

description: This sample is of C.japonica plus-tree (Nishikawa10) in 24 March. It is the third of four biological replicates and second of three Technical replicatesused in this experiment.


title: I0427rep2

description: This sample is of C.japonica plus-tree (Iiyama9) in 27 April. It is the second of four biological replicates and second of three Technical replicatesused in this experiment.


title: I0427rep1

description: This sample is of C.japonica plus-tree (Iiyama9) in 27 April. It is the second of four biological replicates and first of three Technical replicatesused in this experiment.


title: I1007rep3

description: This sample is of C.japonica plus-tree (Iiyama9) in 7 October. It is the second of four biological replicates and third of three Technical replicatesused in this experiment.


title: I0622rep2

description: This sample is of C.japonica plus-tree (Iiyama9) in 22 June. It is the second of four biological replicates and second of three Technical replicatesused in this experiment.


title: I0622rep1

description: This sample is of C.japonica plus-tree (Iiyama9) in 22 June. It is the second of four biological replicates and first of three Technical replicatesused in this experiment.


title: I0324rep3

description: This sample is of C.japonica plus-tree (Iiyama9) in 24 March. It is the second of four biological replicates and Third of three Technical replicatesused in this experiment.


title: I0324rep2

description: This sample is of C.japonica plus-tree (Iiyama9) in 24 March. It is the second of four biological replicates and second of three Technical replicatesused in this experiment.


title: C1007rep3

description: This sample is of C.japonica plus-tree (Chousui8) in 7 October. It is the first of four biological replicates and third of three Technical replicatesused in this experiment.


title: I0622rep3

description: This sample is of C.japonica plus-tree (Iiyama9) in 22 June. It is the second of four biological replicates and third of three Technical replicatesused in this experiment.


title: C1007rep2

description: This sample is of C.japonica plus-tree (Chousui8) in 7 October. It is the first of four biological replicates and second of three Technical replicatesused in this experiment.


title: I0824rep3

description: This sample is of C.japonica plus-tree (Iiyama9) in 24 August. It is the second of four biological replicates and third of three Technical replicatesused in this experiment.


title: I0324rep1

description: This sample is of C.japonica plus-tree (Iiyama9) in 24 March. It is the second of four biological replicates and first of three Technical replicatesused in this experiment.


title: C1007rep1

description: This sample is of C.japonica plus-tree (Chousui8) in 7 October. It is the first of four biological replicates and first of three Technical replicatesused in this experiment.


title: I0427rep3

description: This sample is of C.japonica plus-tree (Iiyama9) in 27 April. It is the second of four biological replicates and third of three Technical replicatesused in this experiment.


title: I1007rep2

description: This sample is of C.japonica plus-tree (Iiyama9) in 7 October. It is the second of four biological replicates and second of three Technical replicatesused in this experiment.


title: I0824rep1

description: This sample is of C.japonica plus-tree (Iiyama9) in 24 August. It is the second of four biological replicates and first of three Technical replicatesused in this experiment.


title: I0824rep2

description: This sample is of C.japonica plus-tree (Iiyama9) in 24 August. It is the second of four biological replicates and second of three Technical replicatesused in this experiment.


title: C0824rep3

description: This sample is of C.japonica plus-tree (Chousui8) in 24 August. It is the first of four biological replicates and third of three Technical replicatesused in this experiment.


title: Old 8

city: Brussel

first: Yacine

last: Bareche

address: Rue Héger-Bordetstraat 1

country: Belgium

institute: Jules Bordet Institute

description: Gene expression data from breast tumor sample

hyb_protocol: Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG133 Plus 2 Array, Affymetrix. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.

scan_protocol: GeneChips were scanned using Affymetrix GeneChip Scanner GCS3000.

data_processing: The probe level intensities were background adjusted using the Robust multi-array analysis (RMA) method followed by quantile normalization as impletemented in the R/Bioconductor package frma.

label: Biotin

source_name: Breast tumor

label_protocol: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (HuGen U133 Plus 2 array, Affymetrix).

growth_protocol: Not applicable

extract_protocol: Trizol extraction of total RNA was performed according to the manufacturer's instructions.

treatment_protocol: Not applicable

tag: gender

value: Female

tag: tumor

value: breast cancer tumor

value: stromal micromilieu

tag: type

value: ductal

tag: age

tag: group

value: old

tag: er

value: Negative

tag: pgr

tag: maximum tumor size (cm)

tag: grade

tag: pt-stage

tag: pn-stage


title: Old 6

tag: tissue


title: Old 7


title: Old 5


title: C0622rep3

description: This sample is of C.japonica plus-tree (Chousui8) in 22 June. It is the first of four biological replicates and third of three Technical replicatesused in this experiment.


title: C0824rep1

description: This sample is of C.japonica plus-tree (Chousui8) in 24 August. It is the first of four biological replicates and first of three Technical replicatesused in this experiment.


title: C0824rep2

description: This sample is of C.japonica plus-tree (Chousui8) in 24 August. It is the first of four biological replicates and second of three Technical replicatesused in this experiment.


title: 7Normal

city: Changsha

first: Wu

last: Hao

state: Hunan

address: 138 Tongzipo Road

department: Department of General Surgery

country: China

institute: The Third Xiangya Hospital of Central South University

hyb_protocol: 1 μg of each labeled cRNA was fragmented by adding 5 μl 10 × Blocking Agent and 1 μl of 25 × Fragmentation Buffer, then heated the mixture at 60°C for 30 min, finally 25 μl 2 × GE Hybridization buffer was added to dilute the labeled cRNA. 50 μl of hybridization solution was dispensed into the gasket slide and assembled to the LncRNA expression microarray slide. The slides were incubated for 17 hours at 65°C in an Agilent Hybridization Oven.

scan_protocol: The hybridized arrays were washed, fixed and scanned with using the Agilent DNA Microarray Scanner (part number G2505C)

data_processing: Agilent Feature Extraction software (version 11.0.1.1) was used to analyze acquired array images. Quantile normalization and subsequent data processing were performed with using the GeneSpring GX v12.1 software package (Agilent Technologies). After quantile normalization of the raw data, LncRNAs and mRNAs that at least 5 out of 10 samples have flags in Present or Marginal (“All Targets Value”) were chosen for further data analysis. Differentially expressed LncRNAs and mRNAs with statistical significance between the two groups were identified through P-value/FDR filtering. Differentially expressed LncRNAs and mRNAs between the two samples were identified through Fold Change filtering. Hierarchical Clustering and combined analysis were performed using homemade scripts.

source_name: 7Normal_matched adjacent non-tumor sample

label_protocol: Sample labeling and array hybridization were performed according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology) with minor modifications. Briefly, mRNA was purified from total RNA after removal of rRNA (mRNA-ONLY™ Eukaryotic mRNA Isolation Kit, Epicentre). Then, each sample was amplified and transcribed into fluorescent cRNA along the entire length of the transcripts without 3' bias utilizing a random priming method (Arraystar Flash RNA Labeling Kit, Arraystar). The labeled cRNAs were purified by RNeasy Mini Kit (Qiagen). The concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000.

extract_protocol: Total RNA were extracted using Trizol. The RNA quantity and quality were measured by NanoDrop ND-1000. RNA integrity was assessed by standard denaturing agarose gel electrophoresis.

value: matched adjacent non-tumor sample

tag: Sex

value: Male

value: 51 years


title: Old 2


title: 6Normal

source_name: 6Normal_matched adjacent non-tumor sample

value: 45 years


title: 8ca

source_name: 8ca_colorectal cancer sample

value: colorectal cancer sample

value: 46 years


title: Old 3


title: Young 7

value: young


title: Young 9


title: C0622rep2

description: This sample is of C.japonica plus-tree (Chousui8) in 22 June. It is the first of four biological replicates and second of three Technical replicatesused in this experiment.


title: Young 3


title: Old 4


title: C0427rep2

description: This sample is of C.japonica plus-tree (Chousui8) in 27 April. It is the first of four biological replicates and second of three Technical replicatesused in this experiment.


title: Young 8


title: Old 1


title: C0427rep3

description: This sample is of C.japonica plus-tree (Chousui8) in 27 April. It is the first of four biological replicates and third of three Technical replicatesused in this experiment.


title: Young 6


title: Young 4


title: C0427rep1

description: This sample is of C.japonica plus-tree (Chousui8) in 27 April. It is the first of four biological replicates and first of three Technical replicatesused in this experiment.


title: C0324rep2

description: This sample is of C.japonica plus-tree (Chousui8) in 24 March. It is the first of four biological replicates and second of three Technical replicatesused in this experiment.


title: 7ca

source_name: 7ca_colorectal cancer sample


title: C0324rep3

description: This sample is of C.japonica plus-tree (Chousui8) in 24 March. It is the first of four biological replicates and Third of three Technical replicatesused in this experiment.


title: Young 2


title: C0622rep1

description: This sample is of C.japonica plus-tree (Chousui8) in 22 June. It is the first of four biological replicates and first of three Technical replicatesused in this experiment.


title: C0324rep1

description: This sample is of C.japonica plus-tree (Chousui8) in 24 March. It is the first of four biological replicates and first of three Technical replicatesused in this experiment.


title: 8Normal

source_name: 8Normal_matched adjacent non-tumor sample


title: 6ca

source_name: 6ca_colorectal cancer sample


title: Young 1


title: Young 5


title: HUVEC_LC50_rep3

status: Public on Nov 25 2016

city: Amsterdam

first: Perry

middle: D

last: Moerland

address: Meibergdreef 9

department: Epidemiology and Data Science

country: Netherlands

institute: Amsterdam UMC

hyb_protocol: Per sample, 750 ng of the obtained biotinylated cRNA samples was hybridized onto the  Illumina HumanHT-12 v4 (Illumina, Inc., San Diego, CA, U.S.A.). Hybridization and washing were performed according to the Illumina Manual “Direct Hybridization Assay  Guide”.

scan_protocol: Scanning was performed on the Illumina iScan (Illumina, Inc., San Diego, CA, U.S.A.).

data_processing: Data were retrieved using Illumina's GenomeStudio v2011.1 software. Analyses were carried out with Bioconductor packages in the statistical software package R (version 3.1.0). Normexp-by-control background correction, quantile normalization, and log2 transformation (Shi et al, NAR, 2010) were performed on the Illumina sample and control probe profiles using the limma package (version 3.20.1), function neqc.

label_protocol: Biotinylated cRNA was prepared using the Illumina TotalPrep RNA Amplification Kit  (Ambion, Inc., Austin, TX, U.S.A.) according to the manufacturer’s specifications with an input of 200 ng  total RNA.

growth_protocol: Human epidermoid carcinoma (A431) cells were cultured in Dulbecco's Modified Eagle's medium (DMEM, Lonza, Walkersville, MD) supplemented with 10% fetal bovine serum (FBS) (Bodinco, Alkmaar, the Netherlands), 1% penicillin/streptomycin, and 1% L-glutamine (both from Lonza). Human umbilical vein endothelial cells (HUVECs) were isolated as described in Post et al. (Exp Cell Res 2013; 319:2501-2513) and maintained in EndoGro-LS complete culture medium (Merck Millipore, Billerica, MA). HUVECs were grown in Primaria cell culture flasks (Corning Life Sciences, Tewksbury, MA). Human perihilar cholangiocarcinoma (SK-ChA-1) cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 culture medium (Lonza) supplemented with 10% FBS, 1% penicillin/streptomycin, 1% L-glutamine, and 1 × 10-5% β-mercaptoethanol. All cells were maintained at standard culture conditions (37 C, 5% CO2, 95% air).

extract_protocol: Total cellular RNA was extracted using 1 mL of TRIzol (Life Technologies, Bleiswijk, the Netherlands) according to the manufacturer’s protocol. RNA samples were purified using the NucleoSpin RNA kit (Machery-Nagel, Düren, Germany) and eluted in 30 µL RNAse-free water. The quality control, RNA labeling, hybridization, and data extraction were performed at ServiceXS (Leiden, the Netherlands). RNA concentration was measured using the Nanodrop ND-1000  spectrophotometer (Nanodrop Technologies, Wilmington, DE, U.S.A). The RNA quality and integrity was  determined using Lab-on-Chip analysis on the Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., Santa  Clara, CA, U.S.A.).

treatment_protocol: Cells were seeded in either 6-well (2 mL per well) culture plates (Corning Life Sciences). HUVEC, SK-ChA-1, and A431 cells were seeded at a density of 0.5 × 10^5 cells/mL, 0.25 × 10^6 cells/mL, and 0.5 × 10^6 cells/mL, respectively, and cultured until confluency. HUVECs were cultured in Primaria culture plates (Corning Life Sciences) throughout the study. Thereafter, cells were washed with PBS and incubated with ZPCLs in serum-free supplemented phenol red-free medium for 1 hour at 37 °C under standard culture conditions. Control cells received an equal volume of buffer. Next, cells were washed with PBS and fresh supplemented phenol red-free medium was added. Cells were either returned to the incubator (control and dark toxicity) or irradiated with a 671-nm diode laser (CNI, Changchun, China) at a laser power of 500 mW with a fluence of 15 J/cm^2. During the application of PDT, cells were placed in the dark and maintained at 37 °C using a hotplate (cat. no. 97042-616, VWR, Radnor, PA).

tag: cell line

value: HUVEC

tag: treatment

tag: slide

tag: slide position

value: G


title: Sk-Cha1_LC50_rep3

value: H


title: Sk-Cha1_LC90_rep3

value: L


title: A431_Control_rep3

value: Control

value: K


title: HUVEC_DT_rep3

source_name: HUVEC,DT

value: DT

value: I


title: Sk-Cha1_DT_rep3

value: J


title: HUVEC_Control_rep3

source_name: HUVEC,Control

value: F


title: A431_LC90_rep3

value: E


title: HUVEC_Control_rep2


title: Sk-Cha1_LC50_rep2


title: A431_DT_rep3

value: B


title: A431_DT_rep2


title: HUVEC_LC50_rep2


title: Sk-Cha1_Control_rep3

value: A


title: A431_DT_rep1


title: A431_Control_rep1


title: Sk-Cha1_Control_rep2

value: D


title: Sk-Cha1_LC50_rep1


title: A431_Control_rep2


title: A431_LC90_rep2

value: C


title: A431_LC50_rep3


title: HUVEC_DT_rep2


title: A431_LC50_rep2


title: Sk-Cha1_LC90_rep2


title: A431_LC50_rep1


title: Sk-Cha1_DT_rep1


title: Sk-Cha1_LC90_rep1


title: HUVEC_LC90_rep2


title: HUVEC_LC90_rep3


title: Sk-Cha1_DT_rep2


title: HUVEC_Control_rep1


title: HUVEC_DT_rep1


title: HUVEC_LC50_rep1


title: Sk-Cha1_Control_rep1


title: A431_LC90_rep1


title: PZAM_replicate2

city: Paris

first: Céline

last: Méhats

address: 24 rue du Faubourg St jacques

country: France

institute: Inserm

description: Gene expression in zone of altered morphology

hyb_protocol: 0.6 ug of Cy3-labelled cRNA (specific activity >10.0 pmol Cy3/ug cRNA) was fragmented at 60°C for 30 minutes in a reaction volume of 25 µl containing 1x Agilent fragmentation buffer and 2x Agilent blocking agent following the manufacturers instructions. On completion of the fragmentation reaction, 25 µl of 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to Agilent Sureprint G3 Human GE 8*60K (G4851A) for 17 hours at 65°C in a rotating Agilent hybridization oven.  After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent) and 1 minute with 37°C GE Wash buffer 2 (Agilent), then dried immediately by brief centrifugation.

scan_protocol: Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505C) using GE1_107_Sep09 scanning protocol (Scan resolution 2um)

data_processing: The scanned images were analyzed with Feature Extraction Software 10.7.3.1 (Agilent) using default parameters (protocol GE1_107_Sep09 and Grid: 028004_D_F_20110325) to obtain background subtracted and spatially detrended Processed Signal intensities.  Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.

source_name: Choriodecidua, zone overlying the cervix between 33-35 weeks of gestation

label_protocol: Cyanine-3 (Cy3) labeled cRNA was prepared from 0.2 ug of Total RNA using the Low Input Quick Amp Labeling Kit according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA).  Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.

extract_protocol: Total RNA was isolated from tissues, firstly crushed in Trizol (Life Technologies, Carlsbad, CA), using a Qiagen RNeasy Kit (Qiagen, Valencia, CA) according to the manufacturer's instructions.

value: choriodecidua

tag: zone

value: overlying the cervix


title: HUVEC_LC90_rep1

value: HUVEC


title: naive CD4+ T cells from HIV+ patient, biological rep1

city: Mexico

first: Gustavo

last: Olvera-García

address: Calzada de Tlalpan 4502

department: Research in Immunology

country: Mexico

institute: National Institute of Respiratory Diseases

description: NATT

hyb_protocol: The labeled cDNA product was heated to 95°C and hybridized to the Human Gene 1.0 ST microarray (Affymetrix Inc.) for 17 + 1 hr at 45°C. The microarrays were then washed at low (6X SSPE) and high (100mM MES, 0.1M NaCl) stringency and stained with streptavidin-phycoerythrin. Fluorescence was amplified by adding biotinylated anti-streptavidin and an additional aliquot of streptavidin-phycoerythrin stain

scan_protocol: A confocal scanner (Affymetrix GeneChip Scanner 3000 7G plus) was used to collect fluorescence signal at 3um resolution after excitation at 570 nm. The average signal from two sequential scans was calculated for each microarray feature

data_processing: The statistical analyses were performed with R 2.11.0. All samples were normalized and summarized by Robust Multiarray Average (RMA). Differential expression analysis between HIV+ patients' and  controls' cells was performed using LIMMA. Log2FC ≥|0.5| and Log (odds)>0 defined differential gene expression.

source_name: Human naive CD4+ T cells_HIV+ patient

label_protocol: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200 ng total RNA

growth_protocol: All RNA samples were extracted ex-vivo after PBMC and specific subpopulation isolation.

extract_protocol: RNA was isolated from human CD4+ T cells subpopulations of HIV+ patients or HIV- controls using RNeasy Mini Kit according to the manufacturer´s directions

tag: subject status

value: HIV+ patient

tag: hiv infection

value: Positive

tag: cell type

value: Human naive CD4+ T (TN) cells


title: XPZAM_replicate2

source_name: Choriodecidua, zone overlying the cervix before 32 weeks of gestation


title: PZAM_replicate1


title: XPZIM_replicate1

description: Gene expression in zone of intact morphology

source_name: Choriodecidua, zone remote from the cervix before 32 weeks of gestation

value: remote zone from the cervix


title: central memory CD4+ T cells from HIV+ patient, biological rep3

source_name: Human central memory CD4+ T cells_HIV+ patient

value: Human central memory CD4+ T (TCM) cells


title: central memory CD4+ T cells from HIV+ patient, biological rep2


title: PZIM_replicate2

source_name: Choriodecidua, zone remote from the cervix betwwen 33 and 35 weeks of gestation


title: XPZAM_replicate1


title: central memory CD4+ T cells from HIV+ patient, biological rep1


title: XPZIM_replicate3


title: ZAM_replicate1

source_name: Choriodecidua, zone overlying the cervix


title: XPZIM_replicate2


title: ZAM_replicate3


title: XPZAM_replicate3


title: naive CD4+ T cells from HIV+ patient, biological rep3

description: NGJ


title: ZAM_replicate2


title: ZIM_replicate2

source_name: Choriodecidua, zone remote from the cervix


title: ZIM_replicate3


title: naive CD4+ T cells from HIV+ patient, biological rep2

description: NCL


title: effector memory CD4+ T cells from control, biological rep3

source_name: Human effector memory CD4+ T cells_control

value: healthy control

value: Negative

value: Human effector memory CD4+ T (TEM) cells


title: effector memory CD4+ T cells from control, biological rep2


title: ZIM_replicate1


title: effector memory CD4+ T cells from control, biological rep1


title: RAW_264_7_LC50_rep1

hyb_protocol: Per sample, 1500 ng of the obtained biotinylated cRNA samples was hybridized onto the  Illumina MouseWG-6 v2 (Illumina, Inc., San Diego, CA, U.S.A.). Hybridization and washing were performed according to the Illumina Manual “Direct Hybridization Assay  Guide”.

growth_protocol: Murine macrophages (RAW 264.7) were cultured in Dulbecco's Modified Eagle's medium (DMEM, Lonza, Walkersville, MD) supplemented with 10% fetal bovine serum (FBS) (Bodinco, Alkmaar, the Netherlands), 1% penicillin/streptomycin, and 1% L-glutamine (both from Lonza). Cells were maintained at standard culture conditions (37 C, 5% CO2, 95% air).

treatment_protocol: Cells were seeded in either 6-well (2 mL per well) culture plates (Corning Life Sciences). were cultured in Primaria culture plates (Corning Life Sciences) throughout the study. RAW 264.7 cells were seeded at a density of 0.5 × 10^6 cells/mL and cultured until confluency. Thereafter, cells were washed with PBS and incubated with ZPCLs in serum-free supplemented phenol red-free medium for 1 hour at 37 °C under standard culture conditions. Control cells received an equal volume of buffer. Next, cells were washed with PBS and fresh supplemented phenol red-free medium was added. Cells were either returned to the incubator (control and dark toxicity) or irradiated with a 671-nm diode laser (CNI, Changchun, China) at a laser power of 500 mW with a fluence of 15 J/cm2. During the application of PDT, cells were placed in the dark and maintained at 37 °C using a hotplate (cat. no. 97042-616, VWR, Radnor, PA).


title: naive CD4+ T cells from control, biological rep1

source_name: Human naive CD4+ T cells_control


title: central memory CD4+ T cells from control, biological rep3

source_name: Human central memory CD4+ T cells_control


title: naive CD4+ T cells from control, biological rep2


title: RAW_264_7_LC90_rep3

value: F


title: RAW_264_7_control_rep1

value: Control


title: RAW_264_7_LC90_rep1


title: naive CD4+ T cells from control, biological rep3


title: RAW_264_7_control_rep2

value: B


title: RAW_264_7_LC90_rep2

value: E


title: RAW_264_7_DT_rep1

value: DT

value: D


title: RAW_264_7_DT_rep2


title: central memory CD4+ T cells from control, biological rep1


title: RAW_264_7_LC50_rep3

value: C


title: RAW_264_7_LC50_rep2


title: RAW_264_7_DT_rep3


title: central memory CD4+ T cells from control, biological rep2


title: RAW_264_7_control_rep3


title: Capsular_0m

status: Public on Nov 24 2016

city: Kunming

first: Ying

last: Zhang

state: Yunnan

address: 935 Jiaoling Rd.

institute: Institute of Medical Biology, Chinese Academy of Medicine Science

description: Gene expression before immunization in peripheral blood from macaque by Capsular

hyb_protocol: 1.65 μl of Cy3-labelled cRNA (specific activity >10.0 pmol Cy3/ug cRNA) was fragmented at 60°C for 30 minutes in a reaction volume of 250 ml containing 1x Agilent fragmentation buffer and 2x Agilent blocking agent following the manufacturers instructions. On completion of the fragmentation reaction, 250 ml of 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to Agilent Whole Human Genome Oligo Microarrays (G4112A) for 17 hours at 65Â°C in a rotating Agilent hybridization oven.  After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent) and 1 minute with 37Â°C GE Wash buffer 2 (Agilent), then dried immediately by brief centrifugation.

scan_protocol: Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 1x44k array slides (Scan Area 61x21.6 mm, Scan resolution 10um, Dye channel is set to Green and Green PMT is set to 100%).

data_processing: The scanned images were analyzed with Feature Extraction Software 9.1 (Agilent) using default parameters (protocol GE1-v1_91 and Grid: 012391_D_20060331) to obtain background subtracted and spatially detrended Processed Signal intensities.  Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.

organism: Macaca mulatta

source_name: Peripheral Blood, Cap, 0m

label_protocol: Cyanine-3 (Cy3) labeled cRNA was prepared from 0.5 ug RNA using the One-Color Low RNA Input Linear Amplification PLUS kit (Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA).  Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.

extract_protocol: PBMCs were isolated from the whole blood by density gradient centrifugation over a Lymphoprep (Ficoll-Paque PREMIUM; GE Healthcare, Piscataway, NJ, USA). Total RNA from the PBMCs was isolated using the TRIzol Reagent (Invitrogen, CA, USA) and purified with the RNeasy Mini Kit (QIAGEN, GmBH, Germany). The RNA Integrity Number (RIN) was evaluated to inspect RNA integrity using an Agilent Bioanalyzer 2100 (Agilent, CA, USA).

value: PBMC

tag: immunization

value: Capsular

tag: time point


title: Capsular_1m

description: Gene expression after 1 month in peripheral blood from macaque immunized by Capsular

source_name: Peripheral Blood, Cap, 1m


title: Hib_1m

description: Gene expression after 1 month in peripheral blood from macaque immunized by Hib conjugate vaccine

source_name: Peripheral Blood, Hib, 1m

value: Hib conjugate


title: 9000 YORK DNA

city: Bethesda

first: Dayna

last: Dreger

state: MD

address: 50 South Drive

department: NHGRI

institute: National Institutes of Health

description: YORK DNA

hyb_protocol: Resuspended DNA was loaded onto BeadChips arrays and hybridized overnight at at 48oC.  Single-base extension and allele-specific staining was performed by hand.

scan_protocol: After allele-specific staining BeadChip arrays were coated with XC4/ethanol , dried for 1 hour and scanned on a BeadArray Reader (Illumina).

data_processing: Image data was analyzed using GenomeStudio with GT module (Illumina).

organism: Canis lupus familiaris

source_name: whole blood

label_protocol: 200ng of genomic DNA was amplified in an overnight reaction at 37oC. The amplified DNA was enzymatically fragmented, precipitated with isopropanol, and resuspended in hybridization buffer.

extract_protocol: Genomic DNA was extracted from whole blood using Proteinase K and phenol/chloroform with standard protocols.

tag: breed

value: Yorkshire Terrier


title: 24315 WOLF DNA

description: WOLF DNA

organism: Canis lupus

value: Wolf


title: 29553 YORK DNA

description: YORK DNA


title: 14953 YORK DNA

description: YORK DNA


title: 14952 YORK DNA

description: YORK DNA


title: 14938 YORK DNA

description: YORK DNA


title: 8997 YORK DNA

description: YORK DNA


title: 24890 WOLF DNA

description: WOLF DNA


title: 27557 YORK DNA

description: YORK DNA


title: 24892 WOLF DNA

description: WOLF DNA


title: 14955 YORK DNA

description: YORK DNA


title: 7572 YORK DNA

description: YORK DNA


title: 24314 WOLF DNA

description: WOLF DNA


title: Toxoid_0m

description: Gene expression before immunization in peripheral blood from macaque by Toxoid

source_name: Peripheral Blood, Tox, 0m

value: Toxoid


title: Hib_0m

description: Gene expression before immunization in peripheral blood from macaque by Hib conjugate vaccine

source_name: Peripheral Blood, Hib, 0m


title: 29556 YORK DNA

description: YORK DNA


title: Toxoid_3m

description: Gene expression after 3 month in peripheral blood from macaque immunized by Toxoid

source_name: Peripheral Blood, Tox, 3m


title: Capsular_2m

description: Gene expression after 2 month in peripheral blood from macaque immunized by Capsular

source_name: Peripheral Blood, Cap, 2m


title: Toxoid_1m

description: Gene expression after 1 month in peripheral blood from macaque immunized by Toxoid

source_name: Peripheral Blood, Tox, 1m


title: Capsular_3m

description: Gene expression after 3 month in peripheral blood from macaque immunized by Capsular

source_name: Peripheral Blood, Cap, 3m


title: Hib_2m

description: Gene expression after 2 month in peripheral blood from macaque immunized by Hib conjugate vaccine

source_name: Peripheral Blood, Hib, 2m


title: Toxoid_2m

description: Gene expression after 2 month in peripheral blood from macaque immunized by Toxoid

source_name: Peripheral Blood, Tox, 2m


title: Hib_3m

description: Gene expression after 3 month in peripheral blood from macaque immunized by Hib conjugate vaccine

source_name: Peripheral Blood, Hib, 3m


title: 24891 WOLF DNA

description: WOLF DNA


title: 24888 WOLF DNA

description: WOLF DNA


title: 10632 WHWT DNA

description: WHWT DNA

value: West Highland White Terrier


title: 10041 WHWT DNA

description: WHWT DNA


title: 10027 WHWT DNA

description: WHWT DNA


title: 8282 WHWT DNA

description: WHWT DNA


title: 9291 WHWT DNA

description: WHWT DNA


title: 28396 WHIP DNA

description: WHIP DNA

value: Whippet


title: 9932 WHIP DNA

description: WHIP DNA


title: 12704 WHWT DNA

description: WHWT DNA


title: 9351 WHWT DNA

description: WHWT DNA


title: 8382 WHWT DNA

description: WHWT DNA


title: 9822 WHIP DNA

description: WHIP DNA


title: 9886 WHIP DNA

description: WHIP DNA


title: 9824 WHIP DNA

description: WHIP DNA


title: 12416 WHWT DNA

description: WHWT DNA


title: 12409 WHWT DNA

description: WHWT DNA


title: 27374 WHIP DNA

description: WHIP DNA


title: 28395 WHIP DNA

description: WHIP DNA


title: 27181 WHIP DNA

description: WHIP DNA


title: PZIM_replicate1

source_name: Choriodecidua, zone remote from the cervix betwwen 33 and 35 weeks of gestation

value: choriodecidua

value: remote zone from the cervix


title: 24889 WOLF DNA

description: WOLF DNA


title: 24634 TURV DNA

description: TURV DNA

value: Belgian Tervuren


title: 24638 TURV DNA

description: TURV DNA


title: 22781 TURV DNA

description: TURV DNA


title: 24635 TURV DNA

description: TURV DNA


title: 24623 TURV DNA

description: TURV DNA


title: 24637 TURV DNA

description: TURV DNA


title: 24619 TURV DNA

description: TURV DNA


title: 9887 WHIP DNA

description: WHIP DNA


title: 9069 TPOO DNA

description: TPOO DNA

value: Toy Poodle


title: 7578 TPOO DNA

description: TPOO DNA


title: 9067 TPOO DNA

description: TPOO DNA


title: 9064 TPOO DNA

description: TPOO DNA


title: 6481 TIBM DNA

description: TIBM DNA

value: Tibetan Mastiff


title: 9079 TPOO DNA

description: TPOO DNA


title: 26331 TPOO DNA

description: TPOO DNA


title: 24636 TURV DNA

description: TURV DNA


title: 27375 WHIP DNA

description: WHIP DNA


title: 27222 TPOO DNA

description: TPOO DNA


title: 22772 TURV DNA

description: TURV DNA


title: 6482 TIBM DNA

description: TIBM DNA


title: 12748 STBD DNA

description: STBD DNA

value: Saint Bernard


title: 12747 STBD DNA

description: STBD DNA


title: 6479 TIBM DNA

description: TIBM DNA


title: 27082 STBD DNA

description: STBD DNA


title: 12749 STBD DNA

description: STBD DNA


title: 27062 STBD DNA

description: STBD DNA


title: 6477 TIBM DNA

description: TIBM DNA


title: 27063 STBD DNA

description: STBD DNA


title: 3145 STAF DNA

description: STAF DNA

value: Staffordshire Bull Terrier


title: 24946 TIBM DNA

description: TIBM DNA


title: 9080 TPOO DNA

description: TPOO DNA


title: 2887 STAF DNA

description: STAF DNA


title: 3144 STAF DNA

description: STAF DNA


title: 3163 STAF DNA

description: STAF DNA


title: 3579 STAF DNA

description: STAF DNA


title: 10439 TPOO DNA

description: TPOO DNA


title: 3139 STAF DNA

description: STAF DNA


title: 6478 TIBM DNA

description: TIBM DNA


title: 1361 STAF DNA

description: STAF DNA


title: 27382 STAF DNA

description: STAF DNA


title: 11494 SSNZ DNA

description: SSNZ DNA

value: Standard Schnauzer


title: 11495 SSNZ DNA

description: SSNZ DNA


title: 11491 SSNZ DNA

description: SSNZ DNA


title: 12750 STBD DNA

description: STBD DNA


title: 11497 SSNZ DNA

description: SSNZ DNA


title: 11496 SSNZ DNA

description: SSNZ DNA


title: 2991 STAF DNA

description: STAF DNA


title: 28480 SSHP DNA

description: SSHP DNA

value: Shetland Sheepdog


title: 27424 SSNZ DNA

description: SSNZ DNA


title: 27148 SSHP DNA

description: SSHP DNA


title: 11493 SSNZ DNA

description: SSNZ DNA


title: 28528 SSHP DNA

description: SSHP DNA


title: 28479 SSHP DNA

description: SSHP DNA


title: 27150 SSHP DNA

description: SSHP DNA


title: 26992 SSHP DNA

description: SSHP DNA


title: 27126 SSHP DNA

description: SSHP DNA


title: 27219 SPOO DNA

description: SPOO DNA

value: Standard Poodle


title: 27225 SPOO DNA

description: SPOO DNA


title: 27218 SPOO DNA

description: SPOO DNA


title: 27440 SSHP DNA

description: SSHP DNA


title: 27216 SPOO DNA

description: SPOO DNA


title: 27151 SPOO DNA

description: SPOO DNA


title: 3137 STAF DNA

description: STAF DNA


title: 27230 SPOO DNA

description: SPOO DNA


title: 27229 SPOO DNA

description: SPOO DNA


title: 27224 SPOO DNA

description: SPOO DNA


title: 27226 SPOO DNA

description: SPOO DNA


title: 3614 SHIH DNA

description: SHIH DNA

value: Shih Tzu


title: 3613 SHIH DNA

description: SHIH DNA


title: 3616 SHIH DNA

description: SHIH DNA


title: 3191 SHIH DNA

description: SHIH DNA


title: 3593 SHIH DNA

description: SHIH DNA


title: 3590 SHIH DNA

description: SHIH DNA


title: 7995 SHAR DNA

description: SHAR DNA

value: Shar Pei


title: 28940 SHAR DNA

description: SHAR DNA


title: 27147 SSHP DNA

description: SSHP DNA


title: 3591 SHIH DNA

description: SHIH DNA


title: 11448 SHAR DNA

description: SHAR DNA


title: 3166 SHIH DNA

description: SHIH DNA


title: 11449 SHAR DNA

description: SHAR DNA


title: 13168 SHAR DNA

description: SHAR DNA


title: 28536 SPOO DNA

description: SPOO DNA


title: 11447 SHAR DNA

description: SHAR DNA


title: 11446 SHAR DNA

description: SHAR DNA


title: 26418 SCOT DNA

description: SCOT DNA

value: Scottish Terrier


title: 11444 SHAR DNA

description: SHAR DNA


title: 11439 SHAR DNA

description: SHAR DNA


title: 11441 SHAR DNA

description: SHAR DNA


title: 24497 SCOT DNA

description: SCOT DNA


title: 24321 SCOT DNA

description: SCOT DNA


title: 9020 SCOT DNA

description: SCOT DNA


title: 20523 SCOT DNA

description: SCOT DNA


title: 8381 SCOT DNA

description: SCOT DNA


title: 3615 SHIH DNA

description: SHIH DNA


title: 15078 SCOT DNA

description: SCOT DNA


title: 3576 SHIH DNA

description: SHIH DNA


title: 27927 SAMO DNA

description: SAMO DNA

value: Samoyed


title: 7975 SCOT DNA

description: SCOT DNA


title: 28349 SCOT DNA

description: SCOT DNA


title: 14510 SAMO DNA

description: SAMO DNA


title: 14507 SAMO DNA

description: SAMO DNA


title: 229 SAMO DNA

description: SAMO DNA


title: 28936 SAMO DNA

description: SAMO DNA


title: 23541 SAMO DNA

description: SAMO DNA


title: 24139 SAMO DNA

description: SAMO DNA


title: 14535 SAMO DNA

description: SAMO DNA


title: 27205 SAMO DNA

description: SAMO DNA


title: 28391 SALU DNA

description: SALU DNA

value: Saluki


title: 27932 SALU DNA

description: SALU DNA


title: 28384 SALU DNA

description: SALU DNA


title: 27915 SALU DNA

description: SALU DNA


title: 27302 SALU DNA

description: SALU DNA


title: 27308 SALU DNA

description: SALU DNA


title: 27317 SALU DNA

description: SALU DNA


title: 13005 ROTT DNA

description: ROTT DNA

value: Rottweiler


title: 27311 SALU DNA

description: SALU DNA


title: 1014 ROTT DNA

description: ROTT DNA


title: 15437 ROTT DNA

description: ROTT DNA


title: 27544 ROTT DNA

description: ROTT DNA


title: 7310 ROTT DNA

description: ROTT DNA


title: 3366 ROTT DNA

description: ROTT DNA


title: 27207 SAMO DNA

description: SAMO DNA


title: 1022 PUG DNA

description: PUG DNA

value: Pug


title: 27132 ROTT DNA

description: ROTT DNA


title: 1027 PUG DNA

description: PUG DNA


title: 27070 ROTT DNA

description: ROTT DNA


title: 1069 PUG DNA

description: PUG DNA


title: 27109 ROTT DNA

description: ROTT DNA


title: 1034 ROTT DNA

description: ROTT DNA


title: 1021 PUG DNA

description: PUG DNA


title: 1183 PUG DNA

description: PUG DNA


title: 1104 PUG DNA

description: PUG DNA


title: 1018 PUG DNA

description: PUG DNA


title: 27314 SALU DNA

description: SALU DNA


title: 27301 SALU DNA

description: SALU DNA


title: 27192 PTWD DNA

description: PTWD DNA

value: Portuguese Water Dog


title: 1030 PUG DNA

description: PUG DNA


title: 8521 POM DNA

description: POM DNA

value: Pomeranian


title: 8522 POM DNA

description: POM DNA


title: 1235 POM DNA

description: POM DNA


title: 6410 POM DNA

description: POM DNA


title: 12763 POM DNA

description: POM DNA


title: 6435 POM DNA

description: POM DNA


title: 6421 POM DNA

description: POM DNA


title: 8520 POM DNA

description: POM DNA


title: 6433 POM DNA

description: POM DNA


title: 1271 POM DNA

description: POM DNA


title: 9017 PEMB DNA

description: PEMB DNA

value: Pembroke Welsh Corgi


title: 7293 PEMB DNA

description: PEMB DNA


title: 1026 PUG DNA

description: PUG DNA


title: 6636 PEMB DNA

description: PEMB DNA


title: 6638 PEMB DNA

description: PEMB DNA


title: 6681 PEMB DNA

description: PEMB DNA


title: 6640 PEMB DNA

description: PEMB DNA


title: 6631 PEMB DNA

description: PEMB DNA


title: 6642 PEMB DNA

description: PEMB DNA


title: 23354 PEKE DNA

description: PEKE DNA

value: Pekingese


title: 28457 PEMB DNA

description: PEMB DNA


title: 22961 PEKE DNA

description: PEKE DNA


title: 22957 PEKE DNA

description: PEKE DNA


title: 23347 PEKE DNA

description: PEKE DNA


title: 27448 PEKE DNA

description: PEKE DNA


title: 23359 PEKE DNA

description: PEKE DNA


title: 24587 PEKE DNA

description: PEKE DNA


title: 9420 PBGV DNA

description: PBGV DNA

value: Petit Basset Griffon Vendeen


title: 23363 PEKE DNA

description: PEKE DNA


title: 22966 PEKE DNA

description: PEKE DNA


title: 9415 PBGV DNA

description: PBGV DNA


title: 9400 PBGV DNA

description: PBGV DNA


title: 9411 PBGV DNA

description: PBGV DNA


title: 9419 PBGV DNA

description: PBGV DNA


title: 9393 PBGV DNA

description: PBGV DNA


title: 14506 PBGV DNA

description: PBGV DNA


title: 7322 PBGV DNA

description: PBGV DNA


title: 1025 PUG DNA

description: PUG DNA


title: 12353 PBGV DNA

description: PBGV DNA


title: 23524 PAPI DNA

description: PAPI DNA

value: Papillon


title: 27882 PAPI DNA

description: PAPI DNA


title: 6630 PEMB DNA

description: PEMB DNA


title: 22901 PAPI DNA

description: PAPI DNA


title: 6205 PAPI DNA

description: PAPI DNA


title: 7262 PAPI DNA

description: PAPI DNA


title: 14935 PAPI DNA

description: PAPI DNA


title: 6685 PAPI DNA

description: PAPI DNA


title: 6014 OES DNA

description: OES DNA

value: Old English Sheepdog


title: 22902 PAPI DNA

description: PAPI DNA


title: 6003 OES DNA

description: OES DNA


title: 5990 OES DNA

description: OES DNA


title: 28462 PAPI DNA

description: PAPI DNA


title: 5957 OES DNA

description: OES DNA


title: 5966 OES DNA

description: OES DNA


title: 5954 OES DNA

description: OES DNA


title: 5984 OES DNA

description: OES DNA


title: 5951 OES DNA

description: OES DNA


title: 5944 OES DNA

description: OES DNA


title: 11478 PBGV DNA

description: PBGV DNA


title: 4657 NOWT DNA

description: NOWT DNA

value: Norwich Terrier


title: 28460 PAPI DNA

description: PAPI DNA


title: 27359 NOWT DNA

description: NOWT DNA


title: 5941 OES DNA

description: OES DNA


title: 24883 NOWT DNA

description: NOWT DNA


title: 7973 NOWT DNA

description: NOWT DNA


title: 8281 NOWT DNA

description: NOWT DNA


title: 27922 NOWT DNA

description: NOWT DNA


title: 7800 NOWT DNA

description: NOWT DNA


title: 27060 NOWT DNA

description: NOWT DNA


title: 22253 NOWT DNA

description: NOWT DNA


title: 24881 NOWT DNA

description: NOWT DNA


title: 7312 NEWF DNA

description: NEWF DNA

value: Newfoundland


title: 57 NEWF DNA

description: NEWF DNA


title: 21473 NEWF DNA

description: NEWF DNA


title: 13580 NEWF DNA

description: NEWF DNA


title: 18 NEWF DNA

description: NEWF DNA


title: 271 NEWF DNA

description: NEWF DNA


title: 6400 NEWF DNA

description: NEWF DNA


title: 28399 NEWF DNA

description: NEWF DNA


title: 23555 NEAP DNA

description: NEAP DNA

value: Neapolitan Mastiff


title: 23554 NEAP DNA

description: NEAP DNA


title: 23567 NEAP DNA

description: NEAP DNA


title: 23550 NEAP DNA

description: NEAP DNA


title: 23551 NEAP DNA

description: NEAP DNA


title: 24693 MSNZ DNA

description: MSNZ DNA

value: Miniature Schnauzer


title: 7672 MSNZ DNA

description: MSNZ DNA


title: 7530 MSNZ DNA

description: MSNZ DNA


title: 24656 MSNZ DNA

description: MSNZ DNA


title: 24692 MSNZ DNA

description: MSNZ DNA


title: 12930 MSNZ DNA

description: MSNZ DNA


title: 25440 MSNZ DNA

description: MSNZ DNA


title: 7671 MSNZ DNA

description: MSNZ DNA


title: 28538 MPOO DNA

description: MPOO DNA

value: Miniature Poodle


title: 28535 MPOO DNA

description: MPOO DNA


title: 12929 MSNZ DNA

description: MSNZ DNA


title: 28542 MPOO DNA

description: MPOO DNA


title: 28526 MPOO DNA

description: MPOO DNA


title: 28525 MPOO DNA

description: MPOO DNA


title: 277 NEWF DNA

description: NEWF DNA


title: 56 NEWF DNA

description: NEWF DNA


title: 28478 MPOO DNA

description: MPOO DNA


title: 28469 MPOO DNA

description: MPOO DNA


title: 27535 MPOO DNA

description: MPOO DNA


title: 23564 NEAP DNA

description: NEAP DNA


title: 13229 MPIN DNA

description: MPIN DNA

value: Miniature Pinscher


title: 12874 MPIN DNA

description: MPIN DNA


title: 27223 MPOO DNA

description: MPOO DNA


title: 12877 MPIN DNA

description: MPIN DNA


title: 12870 MPIN DNA

description: MPIN DNA


title: 12873 MPIN DNA

description: MPIN DNA


title: 12876 MPIN DNA

description: MPIN DNA


title: 12866 MPIN DNA

description: MPIN DNA


title: 11474 MBLT DNA

description: MBLT DNA

value: Miniature Bull Terrier


title: 12928 MSNZ DNA

description: MSNZ DNA


title: 11469 MBLT DNA

description: MBLT DNA


title: 11473 MBLT DNA

description: MBLT DNA


title: 12867 MPIN DNA

description: MPIN DNA


title: 11470 MBLT DNA

description: MBLT DNA


title: 11472 MBLT DNA

description: MBLT DNA


title: 11468 MBLT DNA

description: MBLT DNA


title: 28524 MPOO DNA

description: MPOO DNA


title: 11467 MBLT DNA

description: MBLT DNA


title: 2299A MAST DNA

description: MAST DNA

value: Mastiff


title: 2297B MAST DNA

description: MAST DNA


title: 11463 MBLT DNA

description: MBLT DNA


title: 1016 MAST DNA

description: MAST DNA


title: 11464 MBLT DNA

description: MBLT DNA


title: 11465 MBLT DNA

description: MBLT DNA


title: 24147 MAST DNA

description: MAST DNA


title: 6392 MAST DNA

description: MAST DNA


title: 991 MAST DNA

description: MAST DNA


title: 5512 MAST DNA

description: MAST DNA


title: 29552 LEON DNA

description: LEON DNA

value: Leonberger


title: 12872 MPIN DNA

description: MPIN DNA


title: 2215 LEON DNA

description: LEON DNA


title: 26916 LEON DNA

description: LEON DNA


title: 20260 LEON DNA

description: LEON DNA


title: 15585 MAST DNA

description: MAST DNA


title: 20115 LEON DNA

description: LEON DNA


title: 12869 MPIN DNA

description: MPIN DNA


title: 20045 LEON DNA

description: LEON DNA


title: 20046 LEON DNA

description: LEON DNA


title: 20042 LEON DNA

description: LEON DNA


title: 20093 LEON DNA

description: LEON DNA


title: 12997 LAB DNA

description: LAB DNA

value: Labrador Retriever


title: 11121 LAB DNA

description: LAB DNA


title: 15580 LEON DNA

description: LEON DNA


title: 12019 LAB DNA

description: LAB DNA


title: 11949 LAB DNA

description: LAB DNA


title: 300 LAB DNA

description: LAB DNA


title: 292 LAB DNA

description: LAB DNA


title: 11122 LAB DNA

description: LAB DNA


title: 26996 LAB DNA

description: LAB DNA


title: 7774 KUVZ DNA

description: KUVZ DNA

value: Kuvasz


title: 27179 LAB DNA

description: LAB DNA


title: 7525 KUVZ DNA

description: KUVZ DNA


title: 6605 KUVZ DNA

description: KUVZ DNA


title: 7261 KUVZ DNA

description: KUVZ DNA


title: 2298 MAST DNA

description: MAST DNA


title: 7526 KUVZ DNA

description: KUVZ DNA


title: 6693 KUVZ DNA

description: KUVZ DNA


title: 5341 KUVZ DNA

description: KUVZ DNA


title: 7761 IWSP DNA

description: IWSP DNA

value: Irish Water Spaniel


title: 7575 IWSP DNA

description: IWSP DNA


title: 7569 IWSP DNA

description: IWSP DNA


title: 7556 IWSP DNA

description: IWSP DNA


title: 14517 KUVZ DNA

description: KUVZ DNA


title: 28373 IWSP DNA

description: IWSP DNA


title: 22113 LAB DNA

description: LAB DNA


title: 28372 IWSP DNA

description: IWSP DNA


title: 7590 IWOF DNA

description: IWOF DNA

value: Irish Wolfhound


title: 27438 IWSP DNA

description: IWSP DNA


title: 27029 IWSP DNA

description: IWSP DNA


title: 7522 IWSP DNA

description: IWSP DNA


title: 7616 KUVZ DNA

description: KUVZ DNA


title: 8486 IWOF DNA

description: IWOF DNA


title: 7428 IWOF DNA

description: IWOF DNA


title: 7534 IWOF DNA

description: IWOF DNA


title: 7579 IWOF DNA

description: IWOF DNA


title: 5908 IWOF DNA

description: IWOF DNA


title: 7550 IWOF DNA

description: IWOF DNA


title: 4331 IWOF DNA

description: IWOF DNA


title: 15288 ITGY DNA

description: ITGY DNA

value: Italian Greyhound


title: 28390 ITGY DNA

description: ITGY DNA


title: 28387 ITGY DNA

description: ITGY DNA


title: 7318 KUVZ DNA

description: KUVZ DNA


title: 5924 IWOF DNA

description: IWOF DNA


title: 26972 ITGY DNA

description: ITGY DNA


title: 27353 ITGY DNA

description: ITGY DNA


title: 28389 ITGY DNA

description: ITGY DNA


title: 28487 HUSK DNA

description: HUSK DNA

value: Siberian Husky


title: 29464 HUSK DNA

description: HUSK DNA


title: 28925 HUSK DNA

description: HUSK DNA


title: 28393 ITGY DNA

description: ITGY DNA


title: 11490 HUSK DNA

description: HUSK DNA


title: 27560 IWSP DNA

description: IWSP DNA


title: 11487 HUSK DNA

description: HUSK DNA


title: 27929 HAVA DNA

description: HAVA DNA

value: Havanese


title: 7628 HAVA DNA

description: HAVA DNA


title: 27930 HAVA DNA

description: HAVA DNA


title: 7524 HAVA DNA

description: HAVA DNA


title: 7533 HAVA DNA

description: HAVA DNA


title: 7423 HAVA DNA

description: HAVA DNA


title: 27428 HAVA DNA

description: HAVA DNA


title: 27160 HAVA DNA

description: HAVA DNA


title: 7424 HAVA DNA

description: HAVA DNA


title: 7323 HAVA DNA

description: HAVA DNA


title: 7996 GSNZ DNA

description: GSNZ DNA

value: Giant Schnauzer


title: 16154 GSNZ DNA

description: GSNZ DNA


title: 13276 GSNZ DNA

description: GSNZ DNA


title: 28926 HUSK DNA

description: HUSK DNA


title: 13273 GSNZ DNA

description: GSNZ DNA


title: 13261 GSNZ DNA

description: GSNZ DNA


title: 12317 GSNZ DNA

description: GSNZ DNA


title: 15873 HUSK DNA

description: HUSK DNA


title: 13262 GSNZ DNA

description: GSNZ DNA


title: 13553 GSNZ DNA

description: GSNZ DNA


title: 13267 GSNZ DNA

description: GSNZ DNA


title: 28924 GSMD DNA

description: GSMD DNA

value: Greater Swiss Mountain Dog


title: 21528 GSMD DNA

description: GSMD DNA


title: 15872 HUSK DNA

description: HUSK DNA


title: 25392 GSMD DNA

description: GSMD DNA


title: 11480 HUSK DNA

description: HUSK DNA


title: 12332 GSMD DNA

description: GSMD DNA


title: 21523 GSMD DNA

description: GSMD DNA


title: 3069 GSHP DNA

description: GSHP DNA

value: German Shorthaired Pointer


title: 29550 GSHP DNA

description: GSHP DNA


title: 29551 GSHP DNA

description: GSHP DNA


title: 29549 GSHP DNA

description: GSHP DNA


title: 13235 GSNZ DNA

description: GSNZ DNA


title: 15392 GSHP DNA

description: GSHP DNA


title: 28486 GSD DNA

description: GSD DNA

value: German Shepherd Dog


title: 29548 GSHP DNA

description: GSHP DNA


title: 27023 GSHP DNA

description: GSHP DNA


title: 13151 GSHP DNA

description: GSHP DNA


title: 15394 GSHP DNA

description: GSHP DNA


title: 4371 GSD DNA

description: GSD DNA


title: 28923 GSMD DNA

description: GSMD DNA


title: 13191 GSD DNA

description: GSD DNA


title: 4287 GSD DNA

description: GSD DNA


title: 14567 GSD DNA

description: GSD DNA


title: 27582 GSD DNA

description: GSD DNA


title: 4181 GSD DNA

description: GSD DNA


title: 27108 GSD DNA

description: GSD DNA


title: 14238 GREY DNA

description: GREY DNA

value: Greyhound


title: 14237 GREY DNA

description: GREY DNA


title: 12320 GREY DNA

description: GREY DNA


title: 13678 GREY DNA

description: GREY DNA


title: 12359 GREY DNA

description: GREY DNA


title: 14239 GREY DNA

description: GREY DNA


title: 27064 GSD DNA

description: GSD DNA


title: 10022 GREY DNA

description: GREY DNA


title: 27751 GPYR DNA

description: GPYR DNA

value: Great Pyrenees


title: 28771 GSHP DNA

description: GSHP DNA


title: 12016 GREY DNA

description: GREY DNA


title: 27750 GPYR DNA

description: GPYR DNA


title: 12349 GPYR DNA

description: GPYR DNA


title: 7286 GOLD DNA

description: GOLD DNA

value: Golden Retriever


title: 27057 GOLD DNA

description: GOLD DNA


title: 26997 GOLD DNA

description: GOLD DNA


title: 27005 GOLD DNA

description: GOLD DNA


title: 27014 GOLD DNA

description: GOLD DNA


title: 4154 GSD DNA

description: GSD DNA


title: 27054 GOLD DNA

description: GOLD DNA


title: 27281 GOLD DNA

description: GOLD DNA


title: 27187 GOLD DNA

description: GOLD DNA


title: 4266 GLEN DNA

description: GLEN DNA

value: Glen of Imaal Terrier


title: 4263 GLEN DNA

description: GLEN DNA


title: 12339 GREY DNA

description: GREY DNA


title: 27383 GREY DNA

description: GREY DNA


title: 4253 GLEN DNA

description: GLEN DNA


title: 4257 GLEN DNA

description: GLEN DNA


title: 4250 GLEN DNA

description: GLEN DNA


title: 4261 GLEN DNA

description: GLEN DNA


title: 4246 GLEN DNA

description: GLEN DNA


title: 4262 GLEN DNA

description: GLEN DNA


title: 24316 GDJK DNA

description: GDJK DNA

value: Golden Jackal


title: 24589 GDJK DNA

description: GDJK DNA


title: 4249 GLEN DNA

description: GLEN DNA


title: 10697 FCR DNA

description: FCR DNA

value: Flat Coated Retriever


title: 28379 FCR DNA

description: FCR DNA


title: 27107 FCR DNA

description: FCR DNA


title: 27004 GOLD DNA

description: GOLD DNA


title: 12026 FCR DNA

description: FCR DNA


title: 27140 FCR DNA

description: FCR DNA


title: 27273 FCR DNA

description: FCR DNA


title: 27420 FCR DNA

description: FCR DNA


title: 27007 FCR DNA

description: FCR DNA


title: 27434 GOLD DNA

description: GOLD DNA


title: 13145 FBUL DNA

description: FBUL DNA

value: French Bulldog


title: 7304 FBUL DNA

description: FBUL DNA


title: 28500 FBUL DNA

description: FBUL DNA


title: 16071 FBUL DNA

description: FBUL DNA


title: 14477 FBUL DNA

description: FBUL DNA


title: 16073 FBUL DNA

description: FBUL DNA


title: 27595 FBUL DNA

description: FBUL DNA


title: 14473 FBUL DNA

description: FBUL DNA


title: 27594 FBUL DNA

description: FBUL DNA


title: 13184 ESSP DNA

description: ESSP DNA

value: English Springer Spaniel


title: 13183 ESSP DNA

description: ESSP DNA


title: 4232 ESSP DNA

description: ESSP DNA


title: 4237 ESSP DNA

description: ESSP DNA


title: 4235 ESSP DNA

description: ESSP DNA


title: 4238 ESSP DNA

description: ESSP DNA


title: 28464 ESSP DNA

description: ESSP DNA


title: 4198 ESSP DNA

description: ESSP DNA


title: 28475 ECKR DNA

description: ECKR DNA

value: English Cocker Spaniel


title: 28473 ECKR DNA

description: ECKR DNA


title: 28380 ECKR DNA

description: ECKR DNA


title: 15293 ECKR DNA

description: ECKR DNA


title: 28369 ECKR DNA

description: ECKR DNA


title: 27591 ECKR DNA

description: ECKR DNA


title: 27559 ECKR DNA

description: ECKR DNA


title: 1400 ECKR DNA

description: ECKR DNA


title: 6043 DOBP DNA

description: DOBP DNA

value: Doberman Pinscher


title: 12020 ECKR DNA

description: ECKR DNA


title: 1031 DOBP DNA

description: DOBP DNA


title: 16072 FBUL DNA

description: FBUL DNA


title: 6082 DOBP DNA

description: DOBP DNA


title: 6079 DOBP DNA

description: DOBP DNA


title: 27037 DOBP DNA

description: DOBP DNA


title: 4239 ESSP DNA

description: ESSP DNA


title: 27443 DOBP DNA

description: DOBP DNA


title: 27444 DOBP DNA

description: DOBP DNA


title: 27439 DOBP DNA

description: DOBP DNA


title: 27036 DOBP DNA

description: DOBP DNA


title: 571 DEER DNA

description: DEER DNA

value: Scottish Deerhound


title: 4236 ESSP DNA

description: ESSP DNA


title: 29539 DEER DNA

description: DEER DNA


title: 29537 DEER DNA

description: DEER DNA


title: 29538 DEER DNA

description: DEER DNA


title: 569 DEER DNA

description: DEER DNA


title: 24317 DNGO DNA

description: DNGO DNA

value: Dingo


title: 29532 DEER DNA

description: DEER DNA


title: 28385 DEER DNA

description: DEER DNA


title: 29536 DEER DNA

description: DEER DNA


title: 29531 DEER DNA

description: DEER DNA


title: 24930 DDBX DNA

description: DDBX DNA

value: Dogue de Bordeaux


title: 29535 DEER DNA

description: DEER DNA


title: 24934 DDBX DNA

description: DDBX DNA


title: 6072 DOBP DNA

description: DOBP DNA


title: 24936 DDBX DNA

description: DDBX DNA


title: 24933 DDBX DNA

description: DDBX DNA


title: 25487 DDBX DNA

description: DDBX DNA


title: 24931 DDBX DNA

description: DDBX DNA


title: 15290 DANE DNA

description: DANE DNA

value: Great Dane


title: 21529 DANE DNA

description: DANE DNA


title: 12992 DANE DNA

description: DANE DNA


title: 6694 DANE DNA

description: DANE DNA


title: 13574 DANE DNA

description: DANE DNA


title: 27184 DANE DNA

description: DANE DNA


title: 8267 DACH DNA

description: DACH DNA

value: Dachshund


title: 8241 DACH DNA

description: DACH DNA


title: 8265 DACH DNA

description: DACH DNA


title: 8260 DACH DNA

description: DACH DNA


title: 12993 DANE DNA

description: DANE DNA


title: 26647 DANE DNA

description: DANE DNA


title: 8274 DACH DNA

description: DACH DNA


title: 8244 DACH DNA

description: DACH DNA


title: 8273 DACH DNA

description: DACH DNA


title: 8242 DACH DNA

description: DACH DNA


title: 8257 DACH DNA

description: DACH DNA


title: 11451 COLL DNA

description: COLL DNA

value: Collie


title: 8272 DACH DNA

description: DACH DNA


title: 28482 COLL DNA

description: COLL DNA


title: 28507 COLL DNA

description: COLL DNA


title: 7300 COLL DNA

description: COLL DNA


title: 7298 COLL DNA

description: COLL DNA


title: 7296 COLL DNA

description: COLL DNA


title: 6695 DANE DNA

description: DANE DNA


title: 379 COLL DNA

description: COLL DNA


title: 374 COLL DNA

description: COLL DNA


title: 952 CKCS DNA

description: CKCS DNA

value: Cavalier King Charles Spaniel


title: 7279 CKCS DNA

description: CKCS DNA


title: 27121 CKCS DNA

description: CKCS DNA


title: 15286 CKCS DNA

description: CKCS DNA


title: 14913 CKCS DNA

description: CKCS DNA


title: 7303 COLL DNA

description: COLL DNA


title: 14916 CKCS DNA

description: CKCS DNA


title: 14911 CKCS DNA

description: CKCS DNA


title: 14912 CKCS DNA

description: CKCS DNA


title: 14520 CKCS DNA

description: CKCS DNA


title: 13193 CKCS DNA

description: CKCS DNA


title: 22802 CHOW DNA

description: CHOW DNA

value: Chow Chow


title: 23372 CHOW DNA

description: CHOW DNA


title: 28872 CHOW DNA

description: CHOW DNA


title: 11454 COLL DNA

description: COLL DNA


title: 15628 CHOW DNA

description: CHOW DNA


title: 28871 CHOW DNA

description: CHOW DNA


title: 28880 CHOW DNA

description: CHOW DNA


title: 28885 CHOW DNA

description: CHOW DNA


title: 28874 CHOW DNA

description: CHOW DNA


title: 13167 CHIH DNA

description: CHIH DNA

value: Chihuahua


title: 14923 CHIH DNA

description: CHIH DNA


title: 13132 CHIH DNA

description: CHIH DNA


title: 1204 CHIH DNA

description: CHIH DNA


title: 13129 CHIH DNA

description: CHIH DNA


title: 1206 CHIH DNA

description: CHIH DNA


title: 9284 CARD DNA

description: CARD DNA

value: Cardigan Welsh Corgi


title: 1202 CHIH DNA

description: CHIH DNA


title: 7654 CARD DNA

description: CARD DNA


title: 7976 CARD DNA

description: CARD DNA


title: 7666 CARD DNA

description: CARD DNA


title: 1205 CHIH DNA

description: CHIH DNA


title: 7328 CARD DNA

description: CARD DNA


title: 6199 CARD DNA

description: CARD DNA


title: 27169 CAIR DNA

description: CAIR DNA

value: Cairn Terrier


title: 9283 CARD DNA

description: CARD DNA


title: 6200 CARD DNA

description: CARD DNA


title: 7670 CARD DNA

description: CARD DNA


title: 9330 CAIR DNA

description: CAIR DNA


title: 10026 CAIR DNA

description: CAIR DNA


title: 7802 CAIR DNA

description: CAIR DNA


title: 13037 CAIR DNA

description: CAIR DNA


title: 28521 CAIR DNA

description: CAIR DNA


title: 28517 CAIR DNA

description: CAIR DNA


title: 27172 CAIR DNA

description: CAIR DNA


title: 23695 BULT DNA

description: BULT DNA

value: Bull Terrier


title: 23638 BULT DNA

description: BULT DNA


title: 13291 BULT DNA

description: BULT DNA


title: 28466 CAIR DNA

description: CAIR DNA


title: 9285 CARD DNA

description: CARD DNA


title: 27201 CHIH DNA

description: CHIH DNA


title: 23632 BULT DNA

description: BULT DNA


title: 23631 BULT DNA

description: BULT DNA


title: 23630 BULT DNA

description: BULT DNA


title: 23678 BULT DNA

description: BULT DNA


title: 1125 BULM DNA

description: BULM DNA

value: Bullmastiff


title: 23661 BULT DNA

description: BULT DNA


title: 23674 BULT DNA

description: BULT DNA


title: 1116 BULM DNA

description: BULM DNA


title: 1110 BULM DNA

description: BULM DNA


title: 1107 BULM DNA

description: BULM DNA


title: 1126 BULM DNA

description: BULM DNA


title: 1113 BULM DNA

description: BULM DNA


title: 14500 BULM DNA

description: BULM DNA


title: 13038 CAIR DNA

description: CAIR DNA


title: 3589 BULD DNA

description: BULD DNA

value: Bulldog


title: 3556 BULD DNA

description: BULD DNA


title: 14499 BULD DNA

description: BULD DNA


title: 14476 BULD DNA

description: BULD DNA


title: 26414 BULM DNA

description: BULM DNA


title: 14474 BULD DNA

description: BULD DNA


title: 4208 BSJI DNA

description: BSJI DNA

value: Basenji


title: 28497 BSJI DNA

description: BSJI DNA


title: 27452 BULD DNA

description: BULD DNA


title: 27453 BULD DNA

description: BULD DNA


title: 1198 BULD DNA

description: BULD DNA


title: 4204 BSJI DNA

description: BSJI DNA


title: 4212 BSJI DNA

description: BSJI DNA


title: 12694 BSJI DNA

description: BSJI DNA


title: 20567 BSJI DNA

description: BSJI DNA


title: 4229 BSJI DNA

description: BSJI DNA


title: 23667 BULT DNA

description: BULT DNA


title: 1112 BULM DNA

description: BULM DNA


title: 27182 BSJI DNA

description: BSJI DNA


title: 4207 BSJI DNA

description: BSJI DNA


title: 27387 BSJI DNA

description: BSJI DNA


title: 15420 BRTR DNA

description: BRTR DNA

value: Black Russian Terrier


title: 15424 BRTR DNA

description: BRTR DNA


title: 15419 BRTR DNA

description: BRTR DNA


title: 28920 BULM DNA

description: BULM DNA


title: 13160 BULD DNA

description: BULD DNA


title: 27137 BRIT DNA

description: BRIT DNA

value: Brittany


title: 12726 BRIT DNA

description: BRIT DNA


title: 15422 BRTR DNA

description: BRTR DNA


title: 27117 BRIT DNA

description: BRIT DNA


title: 12724 BRIT DNA

description: BRIT DNA


title: 13150 BRIT DNA

description: BRIT DNA


title: 28919 BRIT DNA

description: BRIT DNA


title: 7780 BRIA DNA

description: BRIA DNA

value: Briard


title: 20240 BRIA DNA

description: BRIA DNA


title: 20268 BRIA DNA

description: BRIA DNA


title: 20212 BRIA DNA

description: BRIA DNA


title: 12721 BRIT DNA

description: BRIT DNA


title: 14565 BRIA DNA

description: BRIA DNA


title: 12720 BRIT DNA

description: BRIT DNA


title: 14502 BRIA DNA

description: BRIA DNA


title: 20217 BRIA DNA

description: BRIA DNA


title: 20022 BRIA DNA

description: BRIA DNA


title: 20026 BRIA DNA

description: BRIA DNA


title: 27587 BRIA DNA

description: BRIA DNA


title: 27749 BOX DNA

description: BOX DNA

value: Boxer


title: 1179 BOX DNA

description: BOX DNA


title: 1177 BOX DNA

description: BOX DNA


title: 28501 BOX DNA

description: BOX DNA


title: 27928 BOX DNA

description: BOX DNA


title: 12966 BOX DNA

description: BOX DNA


title: 12727 BRIT DNA

description: BRIT DNA


title: 12725 BRIT DNA

description: BRIT DNA


title: 8700 BOST DNA

description: BOST DNA

value: Boston Terrier


title: 8701 BOST DNA

description: BOST DNA


title: 27200 BOX DNA

description: BOX DNA


title: 12341 BOST DNA

description: BOST DNA


title: 8704 BOST DNA

description: BOST DNA


title: 27190 BOX DNA

description: BOX DNA


title: 8698 BOST DNA

description: BOST DNA


title: 5413 BOST DNA

description: BOST DNA


title: 997 BOX DNA

description: BOX DNA


title: 5412 BOST DNA

description: BOST DNA


title: 12879 BORZ DNA

description: BORZ DNA

value: Borzoi


title: 28388 BORZ DNA

description: BORZ DNA


title: 965 BOST DNA

description: BOST DNA


title: 27924 BORZ DNA

description: BORZ DNA


title: 13142 BORZ DNA

description: BORZ DNA


title: 8707 BOST DNA

description: BOST DNA


title: 12818 BORZ DNA

description: BORZ DNA


title: 28503 BOX DNA

description: BOX DNA


title: 979 BORZ DNA

description: BORZ DNA


title: 13141 BORZ DNA

description: BORZ DNA


title: 27197 BORZ DNA

description: BORZ DNA


title: 978 BORZ DNA

description: BORZ DNA


title: 27195 BORZ DNA

description: BORZ DNA


title: 11125 BORD DNA

description: BORD DNA

value: Border Collie


title: 12713 BORD DNA

description: BORD DNA


title: 28463 BORD DNA

description: BORD DNA


title: 28459 BORD DNA

description: BORD DNA


title: 28461 BORD DNA

description: BORD DNA


title: 27576 BORD DNA

description: BORD DNA


title: 27103 BORD DNA

description: BORD DNA


title: 27331 BORD DNA

description: BORD DNA


title: 27138 BORD DNA

description: BORD DNA


title: 27913 BMD DNA

description: BMD DNA

value: Bernese Mountain Dog


title: 27914 BMD DNA

description: BMD DNA


title: 28398 BLDH DNA

description: BLDH DNA

value: Bloodhound


title: 950 BOST DNA

description: BOST DNA


title: 11438 BLDH DNA

description: BLDH DNA


title: 11431 BLDH DNA

description: BLDH DNA


title: 11432 BLDH DNA

description: BLDH DNA


title: 24606 BELS DNA

description: BELS DNA

value: Belgian Sheepdog


title: 15646 BELS DNA

description: BELS DNA


title: 25393 BLDH DNA

description: BLDH DNA


title: 28292 BELS DNA

description: BELS DNA


title: 23989 BELS DNA

description: BELS DNA


title: 22739 BELS DNA

description: BELS DNA


title: 28284 BELS DNA

description: BELS DNA


title: 22734 BELS DNA

description: BELS DNA


title: 27579 BORD DNA

description: BORD DNA


title: 1327 BEAG DNA

description: BEAG DNA

value: Beagle


title: 7870 BEAG DNA

description: BEAG DNA


title: 22740 BELS DNA

description: BELS DNA


title: 7907 BEAG DNA

description: BEAG DNA


title: 7862 BEAG DNA

description: BEAG DNA


title: 11436 BLDH DNA

description: BLDH DNA


title: 1186 BLDH DNA

description: BLDH DNA


title: 11433 BLDH DNA

description: BLDH DNA


title: 10375 BASS DNA

description: BASS DNA

value: Basset Hound


title: 7875 BEAG DNA

description: BEAG DNA


title: 14524 BEAG DNA

description: BEAG DNA


title: 10372 BASS DNA

description: BASS DNA


title: 7902 BEAG DNA

description: BEAG DNA


title: 1324 BEAG DNA

description: BEAG DNA


title: 11437 BLDH DNA

description: BLDH DNA


title: 7877 BEAG DNA

description: BEAG DNA


title: 10366 BASS DNA

description: BASS DNA


title: 10351 BASS DNA

description: BASS DNA


title: 10335 BASS DNA

description: BASS DNA


title: 10350 BASS DNA

description: BASS DNA


title: 10342 BASS DNA

description: BASS DNA


title: 10355 BASS DNA

description: BASS DNA


title: 8658 AUST DNA

description: AUST DNA

value: Australian Terrier


title: 10333 BASS DNA

description: BASS DNA


title: 8662 AUST DNA

description: AUST DNA


title: 8661 AUST DNA

description: AUST DNA


title: 8660 AUST DNA

description: AUST DNA


title: 7720 AUST DNA

description: AUST DNA


title: 7721 AUST DNA

description: AUST DNA


title: 7260 AUST DNA

description: AUST DNA


title: 7422 AUST DNA

description: AUST DNA


title: 8659 AUST DNA

description: AUST DNA


title: 7871 BEAG DNA

description: BEAG DNA


title: 27161 AUST DNA

description: AUST DNA


title: 28472 AUSS DNA

description: AUSS DNA

value: Australian Shepherd


title: 28465 AUSS DNA

description: AUSS DNA


title: 13157 AUSS DNA

description: AUSS DNA


title: 27104 AUSS DNA

description: AUSS DNA


title: 27573 AUSS DNA

description: AUSS DNA


title: 11425 AUSS DNA

description: AUSS DNA


title: 11428 AUSS DNA

description: AUSS DNA


title: 10340 BASS DNA

description: BASS DNA


title: 7314 AUSS DNA

description: AUSS DNA


title: 28454 AUSS DNA

description: AUSS DNA


title: 29545 AMAL DNA

description: AMAL DNA

value: Alaskan Malamute


title: 29542 AMAL DNA

description: AMAL DNA


title: 29540 AMAL DNA

description: AMAL DNA


title: 27127 AMAL DNA

description: AMAL DNA


title: 29541 AMAL DNA

description: AMAL DNA


title: 14509 AMAL DNA

description: AMAL DNA


title: 29546 AMAL DNA

description: AMAL DNA


title: 1278 AKIT DNA

description: AKIT DNA

value: Akita


title: 1134 AKIT DNA

description: AKIT DNA


title: 1101 AKIT DNA

description: AKIT DNA


title: 1132 AKIT DNA

description: AKIT DNA


title: 12755 AKIT DNA

description: AKIT DNA


title: 1273 AKIT DNA

description: AKIT DNA


title: 1131 AKIT DNA

description: AKIT DNA


title: 12342 AKIT DNA

description: AKIT DNA


title: 1130 AKIT DNA

description: AKIT DNA


title: 11429 AUSS DNA

description: AUSS DNA


title: 27918 AFGH DNA

description: AFGH DNA

value: Afghan Hound


title: 27567 AFGH DNA

description: AFGH DNA


title: 10625 AFGH DNA

description: AFGH DNA


title: 27917 AFGH DNA

description: AFGH DNA


title: 29543 AMAL DNA

description: AMAL DNA


title: 29544 AMAL DNA

description: AMAL DNA


title: 14486 AFGH DNA

description: AFGH DNA


title: 13118 AFGH DNA

description: AFGH DNA


title: 29547 AMAL DNA

description: AMAL DNA


title: 10622 AFGH DNA

description: AFGH DNA


title: 10624 AFGH DNA

description: AFGH DNA


title: 22932 AFGH DNA

description: AFGH DNA


title: 12692 AKIT DNA

description: AKIT DNA


title: 9032 ACKR DNA

description: ACKR DNA

value: American Cocker Spaniel


title: 12451 ACKR DNA

description: ACKR DNA


title: 27384 AFGH DNA

description: AFGH DNA


title: 12452 ACKR DNA

description: ACKR DNA


title: 9991 ACKR DNA

description: ACKR DNA


title: 12453 ACKR DNA

description: ACKR DNA


title: 9007 ACKR DNA

description: ACKR DNA


title: 27116 ACKR DNA

description: ACKR DNA


title: Normal Donor #10

first: Christopher

last: Hourigan

address: 10 Center Drive

institute: NHLBI

data_processing:
For the normalization the geometric mean of three housekeeping genes: HPRT1, PPIH, TFRC were used
Target gene signals normalized to the geometric mean of housekeeping genes; 2^-deltaCt, where deltaCt = (Ct_Target − Ct_HKG)]
For fold change analysis it compares to median of normal donor values (where undetermined values are considered for this purpose to have a CT of 35)
Series supplementary file fold-change.txt reports AML sample gene expression as compared to the median normal donor expression of that gene

source_name: PBMC

extract_protocol:
Total RNA was extracted using Qiagen All-Prep Kit from ficoll purified peripheral blood mononuclear cells (PBMCs)
400ng of total RNA was reverse-transcribed into cDNA using RT2 First Strand Kit (Qiagen).   PCR assays were performed using SABiosciences custom panel CAPH11327E

value: PBMC

tag: phenotype

value: normal


title: Normal Donor #9


title: Normal Donor #5


title: Normal Donor #8


title: Normal Donor #6


title: Normal Donor #7


title: 9110 ACKR DNA

description: ACKR DNA


title: Normal Donor #3


title: AML Patient #28

value: AML


title: AML Patient #27


title: 9030 ACKR DNA

description: ACKR DNA


title: Normal Donor #1


title: AML Patient #30


title: Normal Donor #2


title: AML Patient #25


title: AML Patient #29


title: AML Patient #26


title: AML Patient #23


title: AML Patient #20


title: AML Patient #19


title: AML Patient #21


title: AML Patient #24


title: AML Patient #22


title: AML Patient #18


title: AML Patient #17


title: AML Patient #15


title: AML Patient #14


title: Normal Donor #4


title: AML Patient #13


title: AML Patient #12


title: AML Patient #11


title: AML Patient #10


title: AML Patient #9


title: AML Patient #7


title: AML Patient #8


title: AML Patient #4


title: AML Patient #16


title: AML Patient #3


title: AML Patient #5


title: AML Patient #6


title: AML Patient #1


title: AML Patient #2


title: R3 14.08.14

city: London

first: Pierre

last: Guermonprez

address: 1st floor NHH, Guy's Campus,

department: CIBCI

country: United Kingdom

institute: King's College London

hyb_protocol: Hybridization cocktails were prepared as recommended by NuGEN and hybridised to Affymetrix Mouse Gene 1.0 ST arrays overnight.

scan_protocol: Arrays were washed and stained using Affymetrix Fluidics Station FS450 and GeneChip Hybridisation Wash Stain kit and scanned in GCS3000 7G scanner with Autoloader.

data_processing: Data was analyzed using Gene Pattern software (Broad Institute)

label_protocol: The Encore Biotin Module (NuGEN) kit was used according to manufacturer's instructions to label the cDNA

growth_protocol: Mice grown under spcific pathogen free conditons until 8 weeks of age were culled and bone marrow extracted under sterile conditions to be sorted by flow cytometry

extract_protocol: Cell pellets were lysed in RLT buffer (Rneasy kit- Qiagen). The NuGen Ovation Pico WTA v2 kit was used to process 1ng RNA per sample into SPIA amolifid cDNA

tag: strain

value: Steady state bone marrow

value: Ly6Chi CD115+ cells

tag: cell type (sorted)


title: R2 14.08.14


title: R1 14.08.14


title: 28296 BELS DNA

description: BELS DNA


title: R2 13.08.14


title: R1 13.08.14


title: R3 13.08.14