title: Patient L069 Diagnostic

description:
COG_LUM_AML_Batch_A_Processed_data.txt
COG_LUM_AML_Batch_A_Raw_Intensities.txt
matrix column prefix: L069_T
hybridized to Illumina Human Omni 2.5M SNP array
HumanOmni2.5-4v1-D

source_name: Patient L069 Diagnostic

value: Patient L069

value: AML


title: Patient L069 Remission

status: Public on Apr 10 2017

type: genomic

city: Philadelphia, PA

first: Richard

last: Aplenc

state: PA

address: 3501 Civic Center Blvd

department: Division of Oncology

country: USA

institute: Children's Hospital of Philadelphia

description:
COG_LUM_AML_Batch_A_Processed_data.txt
COG_LUM_AML_Batch_A_Raw_Intensities.txt
matrix column prefix: L069_N
hybridized to Illumina Human Omni 2.5M SNP array
HumanOmni2.5-4v1-D

hyb_protocol: 20 hours at 48°C, according to manufacturer's instructions

scan_protocol: arrays were scanned using iScan

data_processing:
Genomic DNA extracted from AML diagnostic and remission bone marrow samples and genotyped using Human Omni 2.5M SNP arrays (2.5-4v1-D or 2.5-4v1-H) (Illumina).
Raw image data were saved to a project using Genome Studio (Illumina) and B-Allele Frequencies and Log-R Ratios were exported for analysis using ASCAT in R.

label: cy3 and cy5

molecule: genomic DNA

organism: Homo sapiens

source_name: Patient L069 Remission

label_protocol: 200 ng of genomic DNA was whole-genome amplified in an overnight reaction at 37°C using amplification master mix, according to the manufacturer's instructions

extract_protocol: Genomic DNA was extracted using the DNeasy Blood & Tissues Kit  from Qiagen according to manufacturer's instructions.  DNA concentration was determined by measuring absorbance at 260nm on a Nanodrop2000

tag: individual

tag: disease state

tag: tissue

value: Bone Marrow

tag: patient status

value: Remission


title: Patient L074 Remission

description:
COG_LUM_AML_Batch_A_Processed_data.txt
COG_LUM_AML_Batch_A_Raw_Intensities.txt
matrix column prefix: L074_N
hybridized to Illumina Human Omni 2.5M SNP array
HumanOmni2.5-4v1-D

source_name: Patient L074 Remission

value: Patient L074


title: Patient L068 Diagnostic

description:
COG_LUM_AML_Batch_A_Processed_data.txt
COG_LUM_AML_Batch_A_Raw_Intensities.txt
matrix column prefix: L068_T
hybridized to Illumina Human Omni 2.5M SNP array
HumanOmni2.5-4v1-D

source_name: Patient L068 Diagnostic

value: Patient L068

value: Diagnostic


title: TPK411

type: RNA

city: Minneapolis

first: Todd

middle: P

last: Knutson

state: MN

address: 117 Pleasant St SE

department: Minnesota Supercomputing Institute

institute: University of Minnesota

description: T47D_PR_null_ethanol_onapristone_6h_expt_2_rep_2

hyb_protocol: Standard Illumina hybridization protocol

scan_protocol: Standard Illumina scanning protocol

data_processing: The data were filtered and any probes with a detection P value less than 0.05 were removed. The data was then normalized using log2 transformation and quantile normalization with R using the lumi bioconductor package

label: biotin

molecule: total RNA

source_name: T47D-Y cells stably expressing empty pIRESneo3 plasmid, co-treated with ethanol and onapristone for 6 hours

label_protocol: Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays

growth_protocol: Samples grown in complete medium (MEM, 5% FBS, 1% NEAA, 1% pen/strep, 6ng/ml insulin, 200 ug/ml G418) before 1 day starvation in IMEM medium, then treated in IMEM medium for 6 hr.

extract_protocol: RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.

treatment_protocol: Samples treated in IMEM medium, with or without progestin (10e-8 M) and/or antiprogestin (10e-7 M) for 6 hr.

tag: parental cell line

value: Breast

tag: stable vector integration

tag: treatment

value: ethanol and onapristone

tag: time point

value: 6 hr


title: PC448T

city: Huntsville

first: Marie

middle: K

last: Kirby

state: AL

address: 601 Genome Way NE

institute: HudsonAlpha Institute for Biotechnology

hyb_protocol: hyb was performed using standard manufacture protocol

scan_protocol: scan was performed using standard manufacture protocol

data_processing:
Unmethylated and methylated signal intensities in matrix_signal_intensities.txt Series supplementary file.
We converted data points that were not significant above background intensity to NA. We removed CpGs having greater than 10% missing values prior to normalization. Data was normalized using the Combat R package. Post-ComBat normalization, we observed that Infinium I and Infinium II assays showed two distinct bimodal b-value distributions, so we developed a regression method to convert the type I and type II assays to a single bimondal b-distribution corresponding to Reduced Representation Bisulfite Sequencing b-values. After this conversion, any beta score greater than 1 was assigned a 1, and any beta score less than 0 was assigned a 0. The equations for correction are: Infinium I : RRBS = 0.00209 + 0.4377 x Methyl450 + 0.6303 x Methyl4502 ; Infinium II : RRBS = -0.01146 + 0.2541 x Methyl450 + 0.9832 x Methyl4502

label: Cy5 and cy3

source_name: prostate cancer tissue

label_protocol: Labeling was performed using standard manufacture protocol.

extract_protocol: DNA was extracted from tissue lysate using the Qiagen AllPrep DNA/RNA mini kit

value: prostate cancer tissue

tag: age

tag: ethinicity

value: Caucasian


title: PC452T


title: PC538T


title: Patient L067 Diagnostic

description:
COG_LUM_AML_Batch_A_Processed_data.txt
COG_LUM_AML_Batch_A_Raw_Intensities.txt
matrix column prefix: L067_T
hybridized to Illumina Human Omni 2.5M SNP array
HumanOmni2.5-4v1-D

source_name: Patient L067 Diagnostic

value: Patient L067


title: TPK410

description: T47D_PR_null_ethanol_onapristone_6h_expt_2_rep_1


title: TPK409

description: T47D_PR_null_ethanol_RU486_6h_expt_2_rep_3

source_name: T47D-Y cells stably expressing empty pIRESneo3 plasmid, co-treated with ethanol and RU486, for 6 hours

value: ethanol and RU486


title: Patient L068 Remission

description:
COG_LUM_AML_Batch_A_Processed_data.txt
COG_LUM_AML_Batch_A_Raw_Intensities.txt
matrix column prefix: L068_N
hybridized to Illumina Human Omni 2.5M SNP array
HumanOmni2.5-4v1-D

source_name: Patient L068 Remission


title: PC398T


title: PC370T


title: PC366T


title: TPK406

description: T47D_PR_null_ethanol_progesterone_6h_expt_2_rep_3

source_name: T47D-Y cells stably expressing empty pIRESneo3 plasmid, co-treated with ethanol and progesterone, for 6 hours

value: ethanol and progesterone


title: PC405T


title: TPK407

description: T47D_PR_null_ethanol_RU486_6h_expt_2_rep_1


title: PC265T


title: TPK405

description: T47D_PR_null_ethanol_progesterone_6h_expt_2_rep_2


title: PC485T


title: TPK51

source_name: T47D-Y cells stably expressing empty pIRESneo3 plasmid, treated with ethanol (vehicle), for 6 hours

value: ethanol


title: TPK52

source_name: T47D-Y cells stably expressing empty pIRESneo3 plasmid, treated with R5020 for 6 hours


title: TPK404

description: T47D_PR_null_ethanol_progesterone_6h_expt_2_rep_1


title: TPK50


title: Bortezomib treated sample_1

city: Suzhou

first: Ming

last: Li

address: No. 199 Ren'ai Road

country: China

institute: Soochow University

description: Gene expression of 150 nM Bortezomib-treated TE-1 cells, replicate 1

hyb_protocol: 1 μg of each labeled cRNA was fragmented by adding 11 μl 10 × Blocking Agent and 2.2 μl of 25×Fragmentation Buffer, then heated at 60 °C for 30 min, and finally 55 μl 2 × GE Hybridization buffer was added to dilute the labeled cRNA. 100 μl of hybridization solution was dispensed into the gasket slide and assembled to the gene expression microarray slide. The slides were incubated for 17 hours at 65°C in an Agilent Hybridization Oven. After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent) and 1 minute with 37°C GE Wash buffer 2 (Agilent), then dried immediately by brief centrifugation.

scan_protocol: Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505C) using one color scan setting for 4x180k array slides (Scan Area 61x21.6 mm, Scan resolution 3um, Dye channel is set to Green and Green PMT is set to 100%).

data_processing: Agilent Feature Extraction software (version 11.0.1.1) was used to analyze the acquired array images. Quantile normalization and subsequent data processing were performed with using the GeneSpring GX v11.5 software package (Agilent Technologies). After quantile normalization of the raw data, genes that at least 3 out of 6 samples have flags in Detected (“All Targets Value”) were chosen for further data analysis.

source_name: Bortezomib treated TE-1

label_protocol: Total RNA from each sample was linearly amplified and labeled with Cyanine-3 (Cy3)-UTP. The Labeled cRNAs were purified by RNeasy Mini Kit (Qiagen, Valencia, CA). The concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000.

growth_protocol: TE-1 cells were maintained in RPMI 1640 medium (Hyclone) supplemented with 10% heat-inactivated fetal calf serum (Gibco), containing 100 U/mL penicillin and 100 U/mL streptomycin and were incubated in a 5% CO2 humidified incubator at 37°C.

extract_protocol: RNA was extracted by RNAiso Plus (TAKARA) following the manufacturer's instructions. RNA quantity and quality were measured NanoDrop ND-1000. RNA integrity was assessed using standard denaturing agarose gel electrophoresis.

treatment_protocol: TE-1 cells were seeded in 10-cm dishes and treated with 150 nM Bortezomib in triplicate. After 24 hours of incubation, cells were harvested with RNAiso Plus (TAKARA). Equivalent volume of PBS treated cells were served as controls.

tag: cell line

tag: cell type

value: Esophageal Carcinoma Epithelial Cells


title: Vehicle treated control sample_3

description: Gene expression of vehicle-treated TE-1 cells, replicate 3

source_name: Vehicle treated TE-1


title: PC228T


title: Bortezomib treated sample_2

description: Gene expression of 150 nM Bortezomib-treated TE-1 cells, replicate 2


title: TPK403

description: T47D_PR_null_ethanol_ethanol_6h_expt_2_rep_3

source_name: T47D-Y cells stably expressing empty pIRESneo3 plasmid, co-treated with ethanol and ethanol (vehicle), for 6 hours

value: ethanol and ethanol


title: TPK401

description: T47D_PR_null_ethanol_ethanol_6h_expt_2_rep_1


title: Vehicle treated control sample_1

description: Gene expression of vehicle-treated TE-1 cells, replicate 1


title: Mammarygl_high_rep5

status: Public on Apr 08 2017

city: Barretos

first: Lucas

middle: Tadeu

last: Bidinotto

state: SP

address: Antenor Duarte Vilela, 1331

country: Brazil

institute: Barretos Cancer Hospital

description: Gene expression array of mammary gland in post-natal day 21

hyb_protocol: 1.65 ug of labeled cRNA was hybridized to microarray slides (Whole rat genome-4X44K oligo microarrays-G4112F, Agilent Technologies) at 65°C for 17 hours. Slides were then washed and scanned following Agilent’s recommendations.

scan_protocol: The digitalized images were decoded by Agilent G4900DA Sure Scan Microarray Scanner System.

data_processing: The scanned images were analyzed with Feature Extraction Software v.15.5 using the default protocol (GE1_1105_Oct12 and Grid:014879_D_F_20110531) to obtain background subtracted and spatially detrended Processed Signal intensities.  Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.

organism: Rattus norvegicus

source_name: Mammary gland, zinc supplemented diet, replicate 5

label_protocol: 200 ng of total RNA were hybridized onto whole rat genome 4x44K oligo microarrays (G4131F, Agilent Technologies) using one color (Cy3) Low input Quick Amp labeling kit (Agilent Technologies, Inc. Life Sciences and Chemical Analysis Group, Santa Clara, CA, USA), according to manufacturer instructions. Briefly, total RNA plus spike-in controls were reverse transcribed into double stranded cDNA. The primers used for this reaction contained consecutive thymine bases attached to a T7 promoter that paired at the 5’ end of the first strand of cDNAs. Next, the T7 polymerase was added along with nucleotides labeled with fluorescent cyanine-3 (Cy3) dye, which amplified the anti-sense complementary RNAs (cRNA).

extract_protocol: Total RNA was extracted fromtubular/papillary tumors using the miRNeasy kit (QIAGEN Inc. USA Valencia, CA - USA) according to the manufacturer’s instructions. The extracted RNA was stored at -80°C. RNA was quantified in a spectrophotometer NanoVue (GE Healthcare UK Limited, UK) and the integrity was evaluated using the equipment Agilent 2100 Bioanalyzer (Agilent Technologies, Inc. Life Sciences and Chemical Analysis Group, Santa Clara, CA, USA) under standard conditions.

tag: Sex

value: Female

value: Mammary tumor

value: 180 days


title: Mammarygl_high_rep3

source_name: Mammary gland, zinc supplemented diet, replicate 3


title: PC252T


title: Vehicle treated control sample_2

description: Gene expression of vehicle-treated TE-1 cells, replicate 2


title: Mammarygl_high_rep4

source_name: Mammary gland, zinc supplemented diet, replicate 4


title: Mammarygl_high_rep2

source_name: Mammary gland, zinc supplemented diet, replicate 2


title: Bortezomib treated sample_3

description: Gene expression of 150 nM Bortezomib-treated TE-1 cells, replicate 3


title: PC19T


title: PC47T


title: TPK402

description: T47D_PR_null_ethanol_ethanol_6h_expt_2_rep_2


title: Mammarygl_high_rep1

source_name: Mammary gland, zinc supplemented diet, replicate 1


title: Mammarygl_ctrl_rep4

source_name: Mammary gland, negative control, replicate 4


title: Mammarygl_low_rep5

source_name: Mammary gland, zinc deficitent diet, replicate 5


title: Mammarygl_low_rep3

source_name: Mammary gland, zinc deficitent diet, replicate 3


title: Mammarygl_ctrl_rep3

source_name: Mammary gland, negative control, replicate 3


title: Mammarygl_low_rep4

source_name: Mammary gland, zinc deficitent diet, replicate 4


title: TPK53


title: Mammarygl_ctrl_rep2

source_name: Mammary gland, negative control, replicate 2


title: Mammarygl_ctrl_rep5

source_name: Mammary gland, negative control, replicate 5


title: Mammarygl_low_rep1

source_name: Mammary gland, zinc deficitent diet, replicate 1


title: Mammarygl_ctrl_rep1

source_name: Mammary gland, negative control, replicate 1


title: Mammarygl_low_rep2

source_name: Mammary gland, zinc deficitent diet, replicate 2


title: Heat sensitive 27-rep 2

city: Tainan City

first: Chung-Wen

last: Lin

address: No. 1, University Rd

department: Department of Life Sciences

country: Taiwan

institute: National Cheng Kung University

hyb_protocol: Hybridization was produced with the Quick Amp Labeling Kit, one-color (Agilent Technologies) according the manufacturer’s instructions.

scan_protocol: Slides were scanned at 5 μm resolution with a Agilent Technologies Scanner G2505C scanner.

data_processing: An image analysis and normalization software is used to quantify signal and background intensity for each feature, substantially normalized the data by rank-consistency-filtering LOWESS method.

organism: Brassica oleracea var. italica

source_name: shoot meristem of heat sensitive broccoli, 27 degree C, replicate 2

label_protocol: Cy3-labeled cRNA was produced with the Quick Amp Labeling Kit, one-color (Agilent Technologies) according the manufacturer’s instructions.

growth_protocol: The heat-tolerant and heat-sensitive broccoli were grown in growth chambers in long day condition with 18 h light / 6 h dark. Seeds were germinated in vermiculite for 10 days and transplanted to 9 cm diameter pot in soil for 30 days at 22℃. The plants were then transferred to 15, 22, and 27℃, respectively, for 2 weeks and then extracted the total RNA.

extract_protocol: Total RNA were extracted using QIAGEN RNeasy kit follwed by DNAse treatment. The RNA samples were further purified and concentrated by RNeasy MinElute Cleanup.

value: shoot meristem

tag: genotype

value: heat-sensitive

tag: temperature


title: Heat sensitive 27-rep 1

source_name: shoot meristem of heat sensitive broccoli, 27 degree C, replicate 1


title: Heat sensitive 22-rep 2

source_name: shoot meristem of heat sensitive broccoli, 22 degree C, replicate 2


title: Heat sensitive 15-rep 2

source_name: shoot meristem of heat sensitive broccoli, 15 degree C, replicate 2


title: Heat tolerant 27-rep 1

source_name: shoot meristem of heat tolerant broccoli, 27 degree C, replicate 1

value: heat-tolerant


title: Heat sensitive 27-rep 3

source_name: shoot meristem of heat sensitive broccoli, 27 degree C, replicate 3


title: TPK408

description: T47D_PR_null_ethanol_RU486_6h_expt_2_rep_2

source_name: T47D-Y cells stably expressing empty pIRESneo3 plasmid, co-treated with ethanol and RU486, for 6 hours

value: Breast


title: Heat sensitive 22-rep 1

source_name: shoot meristem of heat sensitive broccoli, 22 degree C, replicate 1


title: Heat tolerant 22-rep 3

source_name: shoot meristem of heat tolerant broccoli, 22 degree C, replicate 3


title: Heat sensitive 22-rep 3

source_name: shoot meristem of heat sensitive broccoli, 22 degree C, replicate 3


title: Heat tolerant 27-rep 3

source_name: shoot meristem of heat tolerant broccoli, 27 degree C, replicate 3


title: Heat sensitive 15-rep 3

source_name: shoot meristem of heat sensitive broccoli, 15 degree C, replicate 3


title: Heat sensitive 15-rep 1

source_name: shoot meristem of heat sensitive broccoli, 15 degree C, replicate 1


title: Heat tolerant 27-rep 2

source_name: shoot meristem of heat tolerant broccoli, 27 degree C, replicate 2


title: Heat tolerant 22-rep 2

source_name: shoot meristem of heat tolerant broccoli, 22 degree C, replicate 2


title: Heat tolerant 22-rep 1

source_name: shoot meristem of heat tolerant broccoli, 22 degree C, replicate 1


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_400minET_rep4 [RE_110915data]

status: Public on Apr 07 2017

city: New York

first: Frederique

last: Ruf-Zamojski

state: NY

address: 1468 Madison Avenue

department: Neurology

institute: Mount Sinai Medical Center

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 22

data_processing:
Results were exported as cycle threshold (Ct) values, and Ct values of target genes were normalized to those of housekeeping gene (HKG) RPS11 (ribosomal protein S11; NM_013725.4). Data were expressed as relative Copy numbers by using the formula, Copy Number = 2500 X 1.93(Ct value of Rps11 - Ct value of gene of interest). No fold changes were calculated.
In the non-normalized data matrix, each Biological Sample N appears in 3 columns with the headers 'SAMPLE N-1', 'SAMPLE N-2', and 'SAMPLE N-3', which correspond to 3 qPCR replicates.
In the Sample data tables, the Copy Number of each Biological Sample was derived from the median Ct value of its 3 qPCR replicates.

label: SYBR Green

organism: Mus musculus

source_name: pituitary gonadotrope-derived cells, 2nM GnRH-pulsed

label_protocol: After reverse-transcription of 1 mg of RNA with the Affinity Script reverse-transcriptase (Agilent, Santa Clara, CA), samples were diluted 1:20 in molecular biology grade H2O (Cellgro, Manassas, VA). Later, SYBR Green qPCR assays were performed (40 cycles) in an ABI Prism 7900HT thermal cycler (Applied Biosystems, Foster City, CA) using 5ml of cDNA template and 5ml of master mix containing the specific primers for the targeted gene, Platinum®Taq DNA polymerase, and the required qPCR buffer, according to manufacturer’s recommendations. Three technical qPCR replicates were run for each biological replicate.

growth_protocol: LβT2 cells were seeded on each tissue culture-treated coverslip and were grown for two days in DMEM supplemented with 10% FBS. Cells were incubated in DMEM supplemented with 2% charcoal-treated FBS and 20mM HEPES overnight before the pulse experiment.

extract_protocol: Coverslips were collected with forceps directly in the chamber and placed immediately in 360 ml guanidium thiocyanate RNA lysis buffer (4M guanidium thiocyanate, 25mM sodium citrate pH 7, 0.5% sarcosyl [N-lauroyl sarcosine], and 0.1M 2-mercaptoethanol). Total RNA was isolated with the “Absolutely RNA 96 microprep” kit (Agilent, Santa Clara, CA) according to the manufacturer's protocol, subjected to an ethanol precipitation to remove salts, and resuspended in elution buffer (Agilent, Santa Clara, CA). RNA concentrations were determined with Quant-iT RiboGreen RNA reagent (Invitrogen, Carlsbad, CA) using a fluorescence microplate reader (SpectraMax M3, Molecular Devices, Sunnyvale, CA).

treatment_protocol: Coverslips were placed in inert coverslip racks and pulse patterns were achieved by moving racks among GnRH, wash and resting solutions in chambers maintained at 37°C in a water bath.

value: immortalized gonadotrope cells


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_400minET_rep5 [RE_110915data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 23


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep3 [RE_110915data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 26


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep2 [RE_110915data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 25


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep5 [RE_110915data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 28


title: Heat tolerant 15-rep 2

source_name: shoot meristem of heat tolerant broccoli, 15 degree C, replicate 2


title: PC97T

source_name: prostate cancer tissue


title: Heat tolerant 15-rep 1

source_name: shoot meristem of heat tolerant broccoli, 15 degree C, replicate 1


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep4 [RE_110915data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 27


title: Sperm_10-month_rep4

city: Québec City

first: Marc-André

last: Sirard

state: Quebec

address: Offfice 2732, 2440 Hochelaga Blvd.

department: Sciences Animales

country: Canada

institute: Université Laval

description: Biological replicats 4 of 4

hyb_protocol: Hybridizations were performed according to the microarray manufacturer’s instructions (Agilent Technologies). Briefly, 1.4 μg of labeled sample (in 40 μL) was mixed with 158 μL of hybridization master mix containing 25 μL of Bovine Hybloc competitor DNA (1 mg/ml) , Applied Genetics Laboratories), 2.6 μL of Agilent 100x Blocking Agent and 130 μL of Agilent 2× HI-RPM Hybridization Buffer (Agilent Technologies). Samples were held at 95°C for 3 min and at 37°C for 30 min followed by addition of 65 μL of Agilent-CGHBlock (final volume 260 μL). The samples were loaded onto the microarray and hybridization was carried out in a hybridization oven (Shel Lab) for 40 h at 65°C and 20 rpm. Washing was carried out according to the microarray manufacturer’s instructions

scan_protocol: Slides were scanned with the PowerScanner (Tecan) and analyzed with Array-Pro Analyzer 6.3 software (MediaCybernetics).

data_processing: The array data analysis methods and downstream analysis pipelines are described in detail in (Saadi et al., 2014). Briefly, after Loess normalization, quantile inter-array scale normalization was performed and fitted to a linear model and thus, Bayesian statistics of differential expression were obtained. Thus, differentially methylated probes were identified using linear models for microarray data (LIMMA).

organism: Bos taurus

source_name: animal #0051 sperm cells

label_protocol: For each sample, 2 μg of DNA was labeled using the Universal Linkage System (ULS) labeling kit (Kreatech Biotechnology) according to the manufacturer’s instructions with minor modifications: 1 μL of Cy-ULS dye was added per 1 μg of genomic DNA adjusted with 10× labeling buffer. The labelling mixture was then held for 30 min at 85°C in a thermocycler, followed by 3 min on ice. Non-reacted ULS-Cy3/5 was removed by purification using a QIAquick PCR Purification Kit and samples were eluted in 23.5 μL nuclease-free water. A 1.5 μL aliquot was used to determine DNA concentration and dye incorporation using the ND- 1000 NanoDrop.

extract_protocol: One unit of extended semen was diluted with 1ml of phosphate buffered saline, pH 7.4, 2% SDS in a 1.5 ml microcentrifuge tube. The mixture was vortexed 5 seconds and centrifuged at 1550g for 5 minutes to pellet the sperms. Following centrifugation the supernatant was aspirated. 450µl of lysis buffer (10mM Tris pH 8.0, 10mM EDTA pH 8.0, 1.5% SDS, 100mM NaCl) followed by the addition of 30µl of 1M dithiothreitol and 20µl of Proteinase K solution (20mg/ml). The solution was incubated for 12-16 hours at 56°C in heater shaker with the speed of 155-157 rpm with horizontal tubes tighten to the plate by tape. Following incubation, 160 µl of saturated NaCl solution was added. The mixture was then vortexed for 3x 5 seconds. The solution was then centrifuged for 10 minutes at 15500g. The supernatant from the liquid pellet was carefully removed and poured in 2ml microtubes. 1ml of chilled (4°C) ethanol was added and mixed gently by hand (20 times) to allow DNA to precipitate and centrifuged 15 minutes ate 16000g at 4°C. The supernatant was removed and discarded. 500µl of chilled (4°C) ethanol 70% was added and the mixture was centrifuged for 5 minutes at 16000g. The supernatant was removed and discarded. 200µl of chilled (4°C) ethanol 50% was added and the mixture was centrifuged for 5 minutes at 16000g. The purified DNA was suspended in 100µl of TE buffer (10mM Tris-HCl, 1mM EDTA, pH 8.0). Quantity and purity were evaluated using a Nanodrop and validated by migration on 0.5% agarose gel at 75 volt for 1 hour.

treatment_protocol: The methylation analysis was performed using EmbryoGENE DNA methylation array (EDMA) platform. The methodology have been thoroughly describef in (Saadi et al., 2014). Briefly, 10ng of DNA sample and spike-in control were cut in ≈160bp fragment using MSEI restriction enzyme. MseLig 12 and MseLig 21 adapters were added to the fragmented gDNA sites. Sample were the digested using methyl sensitive restriction enzyme HpaII, HinP1I and AciI. Only unmethylated fragment have been targeted by those enzyme, leaving only one adapter on the cleaved fragment.      Efficiency of the digestion was confirmed by analyzing the spike-in control added prior to the DNA fragmentation step. For the step, qPCR was performed by preparing a separate mixes containing forward and reverse primer targeting HpaII, HinP1I and AciI site. Undigested spike-in (1/1000 dilution) was used as a positive control and non-template controls were also added as negatives control. The amplification plot and the dissociation curve were evaluated for each primer set separately. The DNA fragmentation was considered successful when the threshold cycle (ct) was greater than 5 when compared to the undigested control.Fragment selection by ligation-mediated PCR  After cleavage by methyl sensitive enzyme, the gDNA was purified by ethanol precipitation and dissolve in nuclease free water. Selective amplification of methylated fragments was performed using two rounds of ligation-mediated PCR (LM-PCR). The PCR products were resolved on 0.8% agarose gel to assess the quality. Then the previously added linker were removed.

value: sperm

value: 10 month

tag: breed

value: Holstein-Friesian

value: 16 month


title: Heat tolerant 15-rep 3

source_name: shoot meristem of heat tolerant broccoli, 15 degree C, replicate 3


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep1 [RE_110915data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 24


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_400minET_rep2 [RE_110915data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 20


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_400minET_rep3 [RE_110915data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 21


title: Sperm_10-month_rep3

description: Biological replicats 3 of 4


title: Sperm_12-month_rep3

description: Biological replicats 3 of 4

source_name: animal #2915 sperm cells

value: 12 month


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_400minET_rep1 [RE_110915data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 19


title: Sperm_10-month_rep2

description: Biological replicats 2 of 4

source_name: animal #0032 sperm cells


title: Sperm_12-month_rep1

description: Biological replicats 1 of 4

source_name: animal #0085 sperm cells


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_380minET_rep2 [RE_110915data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 15


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_380minET_rep3 [RE_110915data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 16


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_380minET_rep1 [RE_110915data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 14


title: Low_Insulin_2

description: Biological replicats 2 of 4

hyb_protocol: Hybridizations were performed according to the microarray manufacturer’s instructions (Agilent Technologies). Briefly, 1.4 μg of labeled sample (in 40 μL) was mixed with 158 μL of hybridization master mix containing 25 μL of Bovine Hybloc competitor DNA (1 mg/ml) , (Applied Genetics Laboratories), 2.6 μL of Agilent 100x Blocking Agent and 130 μL of Agilent 2× HI-RPM Hybridization Buffer (Agilent Technologies). Samples were held at 95°C for 3 min and at 37°C for 30 min followed by addition of 65 μL of Agilent-CGHBlock (final volume 260 μL). The samples were loaded onto the microarray and hybridization was carried out in a hybridization oven (Shel Lab) for 40 h at 65°C and 20 rpm. Washing was carried out according to the microarray manufacturer’s instructions.

source_name: control, no treatment

growth_protocol: Briefly, abattoir-derived cumulus oocyte complexes (COCs) (n=882) were matured in groups of 30-40 in vitro for 22 h in 500 µl serum-free maturation medium (IVM). IVM medium, consisted of bicarbonate-buffered TCM199 (M2154) supplemented with 0.68 mM L-glutamine (G8540), 0.5 mg/mL FSH and 0.1 mg/mL LH (Stimufol; PARTNAR Animal Health, Port Huron, Canada), 50 mg/mL gentamicin and 0.4% w/v BSA. Bovine insulin (I5500) was added to the medium at either 0.1 µg/mL (INS0.1) or 10 µg/mL (INS10). A control group without insulin supplementation (INS0) was run in parallel during each production week, medium. Matured oocytes were fertilized in vitro with sperm from a dairy bull with proven field and in vitro fertility and presumed zygotes were incubated under humidified atmosphere of 5 % CO₂, 5% O₂ and 90 % N₂ at 38.5°C in 500 μl modified synthetic oviductual fluid per well until Day 8. Resulting blastocysts (BC8) were evaluated (developmental stage and quality grade) and washed three times in PBS-PVA before individually frozen at -80°C.

extract_protocol: gDNA was extracted from pools of 10 blastocysts using the AllPrep DNA/RNA micro kit (Qiagen). The methylation analysis was performed using EmbryoGENE DNA methylation array (EDMA) platform. The methodology have been thoroughly describef in (Saadi et al., 2014). Briefly, 10ng of DNA sample and spike-in control were cut in ≈160bp fragment using MSEI restriction enzyme. MseLig 12 and MseLig 21 adapters were added to the fragmented gDNA sites. Sample were the digested using methyl sensitive restriction enzyme HpaII, HinP1I and AciI (FastDigesttm). After cleavage by methyl sensitive enzyme, the gDNA was purified by ethanol precipitation and dissolve in nuclease free water. Selective amplification of methylated fragments was performed using two rounds of ligation-mediated PCR (LM-PCR). The PCR products were resolved on 0.8% agarose gel to assess the quality. Then the previously added linker were removed.

treatment_protocol: Bovine insulin (I5500, Sigma-Aldrich, Stockholm, Sweden) was added to the medium during the IVM period of 22 hours, at either 0.1 µg/mL (INS0.1) or 10 µg/mL (INS10). The two different insulin concentrations INS10 and INS0.1 were used in this study in purpose to investigate a potential dose-dependent effect of insulin during oocyte maturation.

value: blastocysts

source_name: Low insulin (0.1ug/ml)


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_380minET_rep4 [RE_110915data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 17


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_360minET_rep3 [RE_110915data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 11


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_360minET_rep4 [RE_110915data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 12


title: Sperm_12-month_rep4

description: Biological replicats 4 of 4


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_360minET_rep1 [RE_110915data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 9


title: Sperm_12-month_rep2

description: Biological replicats 2 of 4


title: Low_Insulin_4

description: Biological replicats 4 of 4


title: 5min pulse_2nM GnRH_2hT_collect_pulse3_480minET_rep4 [RE_110915data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 7


title: 5min pulse_2nM GnRH_2hT_collect_pulse3_480minET_rep2 [RE_110915data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 5


title: High_Insulin_3

description: Biological replicats 3 of 4

source_name: High insulin (10ug/ml)


title: Low_Insulin_3

description: Biological replicats 3 of 4


title: 5min pulse_2nM GnRH_2hT_collect_pulse3_480minET_rep3 [RE_110915data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 6


title: No pulse_0nM GnRH_0hT__0minET_rep2 [RE_110915data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 2

source_name: pituitary gonadotrope-derived cells, no pulse


title: 5min pulse_2nM GnRH_2hT_collect_pulse3_480minET_rep1 [RE_110915data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 4


title: High_Insulin_4

description: Biological replicats 4 of 4


title: No pulse_0nM GnRH_0hT__0minET_rep1 [RE_110915data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 1


title: No pulse_0nM GnRH_0hT__0minET_rep3 [RE_110915data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 3


title: Low_Insulin_1

description: Biological replicats 1 of 4


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_360minET_rep2 [RE_110915data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 10


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_480minET_rep1 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 121

source_name: pituitary gonadotrope-derived cells, 0.08nM GnRH-pulsed


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_480minET_rep3 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 123


title: Sperm_10-month_rep1

description: Biological replicats 1 of 4


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_480minET_rep2 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 122


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_480minET_rep4 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 124


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_480minET_rep5 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 125


title: High_Insulin_1

description: Biological replicats 1 of 4


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_440minET_rep4 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 114


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_460minET_rep5 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 120


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_440minET_rep2 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 112


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_460minET_rep4 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 119


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_440minET_rep1 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 111


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_460minET_rep2 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 117


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_460minET_rep3 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 118


title: Continuous_0.08nM GnRH_2hT_collect_pulse3_400minET_rep4 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 104


title: Continuous_0.08nM GnRH_2hT_collect_pulse3_400minET_rep2 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 102


title: Continuous_0.08nM GnRH_2hT_collect_pulse3_400minET_rep1 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 101


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_460minET_rep1 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 116


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_440minET_rep5 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 115


title: High_Insulin_2

description: Biological replicats 2 of 4


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_480minET_rep3 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 98


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_480minET_rep1 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 96


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_420minET_rep4 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 109


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_420minET_rep3 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 108


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_420minET_rep2 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 107


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_460minET_rep5 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 95


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_480minET_rep2 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 97


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_420minET_rep5 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 110


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_460minET_rep1 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 91


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_440minET_rep5 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 90


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_460minET_rep3 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 93


title: Continuous_0.08nM GnRH_2hT_collect_pulse3_400minET_rep5 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 105


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_380minET_rep5 [RE_110915data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 18


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_420minET_rep1 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 106


title: Continuous_0.08nM GnRH_2hT_collect_pulse3_400minET_rep3 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 103


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_440minET_rep3 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 88


title: 5min pulse_2nM GnRH_2hT_collect_pulse3_480minET_rep5 [RE_110915data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 8


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_480minET_rep4 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 99


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_440minET_rep3 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 113


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_460minET_rep4 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 94


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_440minET_rep2 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 87


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_460minET_rep2 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 92


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_480minET_rep5 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 100


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep4 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 84


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep3 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 83


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_440minET_rep4 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 89


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_440minET_rep1 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 86


title: 10min pulse_2nM GnRH_2hT_collect_pulse3_400minET_rep5 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 80


title: 10min pulse_2nM GnRH_2hT_collect_pulse3_400minET_rep2 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 77


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_360minET_rep5 [RE_110915data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 13


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep2 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 82


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep5 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 85


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_480minET_rep4 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 74


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_480minET_rep5 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 75


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep1 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 81


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_480minET_rep3 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 73


title: 10min pulse_2nM GnRH_2hT_collect_pulse3_400minET_rep3 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 78


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_480minET_rep2 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 72


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_460minET_rep5 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 70


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_440minET_rep4 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 64


title: 10min pulse_2nM GnRH_2hT_collect_pulse3_400minET_rep4 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 79


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_440minET_rep2 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 62


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_460minET_rep2 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 67


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_460minET_rep3 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 68


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_460minET_rep4 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 69


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_440minET_rep3 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 63


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_440minET_rep5 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 65


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep4 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 59


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep5 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 60


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_460minET_rep1 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 66


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_440minET_rep1 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 61


title: 10min pulse_2nM GnRH_2hT_collect_pulse3_400minET_rep1 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 76


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep3 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 58


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep1 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 56


title: 5min pulse_2nM GnRH_2hT_collect_pulse3_400minET_rep2 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 52


title: 5min pulse_2nM GnRH_2hT_collect_pulse3_400minET_rep3 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 53


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_480minET_rep1 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 71


title: 5min pulse_2nM GnRH_2hT_collect_pulse3_400minET_rep4 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 54


title: 5min pulse_2nM GnRH_2hT_collect_pulse3_400minET_rep1 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 51


title: 5min pulse_2nM GnRH_2hT_collect_pulse3_400minET_rep5 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 55


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_480minET_rep3 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 48

source_name: pituitary gonadotrope-derived cells, vehicle-pulsed


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_480minET_rep4 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 49


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_480minET_rep5 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 50


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep2 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 57


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_460minET_rep3 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 43


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_480minET_rep1 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 46


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_460minET_rep2 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 42


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_460minET_rep1 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 41


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_460minET_rep5 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 45


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_440minET_rep5 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 40


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_420minET_rep5 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 35


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_440minET_rep2 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 37


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_480minET_rep2 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 47


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_440minET_rep1 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 36


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_460minET_rep4 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 44


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_440minET_rep3 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 38


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_420minET_rep3 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 33


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_420minET_rep2 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 32


title: 5min pulse_0nM GnRH_2hT_collect_pulse3_400minET_rep5 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 30


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_420minET_rep1 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 31


title: 5min pulse_0nM GnRH_2hT_collect_pulse3_400minET_rep4 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 29


title: No pulse_0nM GnRH_0hT_collect_pulse4_480minET_rep4 [113015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 24


title: 5min pulse_0nM GnRH_2hT_collect_pulse3_400minET_rep3 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 28


title: No pulse_0nM GnRH_0hT_collect_pulse4_480minET_rep5 [113015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 25


title: 5min pulse_0nM GnRH_2hT_collect_pulse3_400minET_rep2 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 27


title: No pulse_0nM GnRH_0hT_collect_pulse4_480minET_rep3 [113015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 23


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_440minET_rep4 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 39


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_420minET_rep4 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 34


title: No pulse_0nM GnRH_0hT_collect_pulse4_460minET_rep4 [113015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 19


title: No pulse_0nM GnRH_0hT_collect_pulse4_460minET_rep5 [113015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 20


title: No pulse_0nM GnRH_0hT_collect_pulse4_480minET_rep1 [113015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 21


title: No pulse_0nM GnRH_0hT_collect_pulse4_480minET_rep2 [113015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 22


title: No pulse_0nM GnRH_0hT_collect_pulse4_460minET_rep3 [113015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 18


title: No pulse_0nM GnRH_0hT_collect_pulse4_440minET_rep2 [113015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 12


title: No pulse_0nM GnRH_0hT_collect_pulse4_420minET_rep3 [113015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 8


title: No pulse_0nM GnRH_0hT_collect_pulse4_440minET_rep3 [113015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 13


title: No pulse_0nM GnRH_0hT_collect_pulse4_440minET_rep4 [113015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 14


title: No pulse_0nM GnRH_0hT_collect_pulse4_420minET_rep5 [113015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 10


title: No pulse_0nM GnRH_0hT_collect_pulse4_460minET_rep2 [113015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 17


title: No pulse_0nM GnRH_0hT_collect_pulse3_400minET_rep4 [113015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 4


title: No pulse_0nM GnRH_0hT_collect_pulse4_440minET_rep1 [113015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 11


title: 5min pulse_0nM GnRH_2hT_collect_pulse3_400minET_rep1 [113015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 26


title: No pulse_0nM GnRH_0hT_collect_pulse3_400minET_rep5 [113015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 5


title: No pulse_0nM GnRH_0hT_collect_pulse4_460minET_rep1 [113015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 16


title: No pulse_0nM GnRH_0hT_collect_pulse4_420minET_rep4 [113015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 9


title: No pulse_0nM GnRH_0hT_collect_pulse4_420minET_rep1 [113015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 6


title: No pulse_0nM GnRH_0hT_collect_pulse4_420minET_rep2 [113015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 7


title: No pulse_0nM GnRH_0hT_collect_pulse3_400minET_rep3 [113015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 3


title: No pulse_0nM GnRH_0hT_collect_pulse4_440minET_rep5 [113015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 15


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_480minET_rep4 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 119


title: No pulse_0nM GnRH_0hT_collect_pulse3_400minET_rep1 [113015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 1


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_460minET_rep4 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 114


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_460minET_rep5 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 115


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_480minET_rep3 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 118


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_480minET_rep1 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 116


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_480minET_rep5 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 120


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_460minET_rep3 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 113


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_460minET_rep2 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 112


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_440minET_rep5 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 110


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_480minET_rep2 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 117


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_460minET_rep1 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 111


title: No pulse_0nM GnRH_0hT_collect_pulse3_400minET_rep2 [113015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 2


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_440minET_rep1 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 106


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_420minET_rep4 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 104


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_420minET_rep5 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 105


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_440minET_rep4 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 109


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_420minET_rep2 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 102


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_440minET_rep2 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 107


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_440minET_rep3 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 108


title: Continuous_0.08nM GnRH_2hT_collect_pulse3_400minET_rep4 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 99


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_420minET_rep3 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 103


title: Continuous_0.08nM GnRH_2hT_collect_pulse3_400minET_rep5 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 100


title: Continuous_0.08nM GnRH_2hT_collect_pulse3_400minET_rep1 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 96


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_480minET_rep3 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 93


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_480minET_rep5 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 95


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_480minET_rep2 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 92


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_460minET_rep5 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 90


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_460minET_rep1 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 86


title: Continuous_0.08nM GnRH_2hT_collect_pulse4_420minET_rep1 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 101


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_480minET_rep4 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 94


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_440minET_rep3 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 83


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_440minET_rep4 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 84


title: Continuous_0.08nM GnRH_2hT_collect_pulse3_400minET_rep3 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 98


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_460minET_rep2 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 87


title: Continuous_0.08nM GnRH_2hT_collect_pulse3_400minET_rep2 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 97


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_460minET_rep3 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 88


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_440minET_rep5 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 85


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_460minET_rep4 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 89


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep1 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 77


title: 10min pulse_2nM GnRH_2hT_collect_pulse3_400minET_rep3 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 75


title: 10min pulse_2nM GnRH_2hT_collect_pulse3_400minET_rep1 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 73


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep3 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 79


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_480minET_rep1 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 91


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep2 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 78


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_440minET_rep1 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 81


title: 10min pulse_2nM GnRH_2hT_collect_pulse3_400minET_rep4 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 76


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep4 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 80


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_480minET_rep2 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 70


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_480minET_rep3 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 71


title: 10min pulse_2nM GnRH_2hT_collect_pulse3_400minET_rep2 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 74


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_480minET_rep4 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 72


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_440minET_rep2 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 82


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_460minET_rep1 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 65


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_440minET_rep4 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 64


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_460minET_rep4 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 68


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_460minET_rep3 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 67


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_480minET_rep1 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 69


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep1 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 56


title: 5min pulse_2nM GnRH_2hT_collect_pulse3_400minET_rep5 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 55


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep2 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 57


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_460minET_rep2 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 66


title: 5min pulse_2nM GnRH_2hT_collect_pulse3_400minET_rep4 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 54


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_440minET_rep2 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 62


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_440minET_rep1 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 61


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_440minET_rep3 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 63


title: 5min pulse_2nM GnRH_2hT_collect_pulse3_400minET_rep2 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 52


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep4 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 59


title: Veh_0nM GnRH_2hT_collect_pulse4_480minET_rep4 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 49


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep5 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 60


title: 5min pulse_2nM GnRH_2hT_collect_pulse3_400minET_rep3 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 53


title: Veh_0nM GnRH_2hT_collect_pulse4_460minET_rep4 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 44


title: 5min pulse_2nM GnRH_2hT_collect_pulse3_400minET_rep1 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 51


title: Veh_0nM GnRH_2hT_collect_pulse4_480minET_rep2 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 47


title: Veh_0nM GnRH_2hT_collect_pulse4_460minET_rep5 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 45


title: Veh_0nM GnRH_2hT_collect_pulse4_460minET_rep3 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 43


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep3 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 58


title: Veh_0nM GnRH_2hT_collect_pulse4_480minET_rep5 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 50


title: Veh_0nM GnRH_2hT_collect_pulse4_480minET_rep1 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 46


title: Veh_0nM GnRH_2hT_collect_pulse4_440minET_rep4 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 39


title: Veh_0nM GnRH_2hT_collect_pulse4_440minET_rep2 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 37


title: Veh_0nM GnRH_2hT_collect_pulse4_480minET_rep3 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 48


title: Veh_0nM GnRH_2hT_collect_pulse4_440minET_rep3 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 38


title: Veh_0nM GnRH_2hT_collect_pulse4_420minET_rep5 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 35


title: Veh_0nM GnRH_2hT_collect_pulse4_460minET_rep2 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 42


title: Veh_0nM GnRH_2hT_collect_pulse4_420minET_rep1 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 31


title: Veh_0nM GnRH_2hT_collect_pulse4_420minET_rep2 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 32


title: Veh_0nM GnRH_2hT_collect_pulse4_440minET_rep5 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 40


title: Veh_0nM GnRH_2hT_collect_pulse3_400minET_rep5 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 30


title: Veh_0nM GnRH_2hT_collect_pulse4_440minET_rep1 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 36


title: Veh_0nM GnRH_2hT_collect_pulse4_420minET_rep4 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 34


title: Veh_0nM GnRH_2hT_collect_pulse3_400minET_rep2 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 27


title: Veh_0nM GnRH_2hT_collect_pulse3_400minET_rep3 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 28


title: Veh_0nM GnRH_2hT_collect_pulse3_400minET_rep4 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 29


title: Veh_0nM GnRH_2hT_collect_pulse4_460minET_rep1 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 41


title: No pulse_0nM GnRH_0hT_collect_pulse4_480minET_rep5 [112015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 25


title: No pulse_0nM GnRH_0hT_collect_pulse4_480minET_rep4 [112015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 24


title: No pulse_0nM GnRH_0hT_collect_pulse4_480minET_rep3 [112015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 23


title: No pulse_0nM GnRH_0hT_collect_pulse4_480minET_rep1 [112015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 21


title: No pulse_0nM GnRH_0hT_collect_pulse4_460minET_rep5 [112015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 20


title: No pulse_0nM GnRH_0hT_collect_pulse4_480minET_rep2 [112015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 22


title: No pulse_0nM GnRH_0hT_collect_pulse4_440minET_rep5 [112015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 15


title: No pulse_0nM GnRH_0hT_collect_pulse4_460minET_rep3 [112015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 18


title: No pulse_0nM GnRH_0hT_collect_pulse4_440minET_rep2 [112015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 12


title: No pulse_0nM GnRH_0hT_collect_pulse4_460minET_rep1 [112015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 16


title: No pulse_0nM GnRH_0hT_collect_pulse4_440minET_rep3 [112015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 13


title: No pulse_0nM GnRH_0hT_collect_pulse4_440minET_rep4 [112015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 14


title: No pulse_0nM GnRH_0hT_collect_pulse4_440minET_rep1 [112015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 11


title: No pulse_0nM GnRH_0hT_collect_pulse4_420minET_rep2 [112015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 7


title: Veh_0nM GnRH_2hT_collect_pulse3_400minET_rep1 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 26


title: Veh_0nM GnRH_2hT_collect_pulse4_420minET_rep3 [112015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 33


title: No pulse_0nM GnRH_0hT_collect_pulse4_420minET_rep5 [112015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 10


title: No pulse_0nM GnRH_0hT_collect_pulse4_420minET_rep1 [112015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 6


title: No pulse_0nM GnRH_0hT_collect_pulse3_400minET_rep4 [112015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 4


title: No pulse_0nM GnRH_0hT_collect_pulse3_400minET_rep5 [112015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 5


title: Continuous_2nM GnRH_2hT_collect_pulse4_480minET_rep2 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 122


title: Continuous_2nM GnRH_2hT_collect_pulse4_480minET_rep5 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 125


title: No pulse_0nM GnRH_0hT_collect_pulse4_460minET_rep2 [112015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 17


title: Continuous_2nM GnRH_2hT_collect_pulse4_480minET_rep1 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 121


title: Continuous_2nM GnRH_2hT_collect_pulse4_480minET_rep4 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 124


title: No pulse_0nM GnRH_0hT_collect_pulse3_400minET_rep2 [112015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 2


title: Continuous_2nM GnRH_2hT_collect_pulse4_460minET_rep3 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 118


title: No pulse_0nM GnRH_0hT_collect_pulse3_400minET_rep1 [112015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 1


title: No pulse_0nM GnRH_0hT_collect_pulse4_420minET_rep4 [112015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 9


title: Continuous_2nM GnRH_2hT_collect_pulse4_460minET_rep4 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 119


title: No pulse_0nM GnRH_0hT_collect_pulse4_420minET_rep3 [112015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 8


title: Continuous_2nM GnRH_2hT_collect_pulse4_460minET_rep2 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 117


title: No pulse_0nM GnRH_0hT_collect_pulse4_460minET_rep4 [112015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 19


title: Continuous_2nM GnRH_2hT_collect_pulse4_480minET_rep3 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 123


title: Continuous_2nM GnRH_2hT_collect_pulse4_460minET_rep1 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 116


title: Continuous_2nM GnRH_2hT_collect_pulse4_440minET_rep5 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 115


title: Continuous_2nM GnRH_2hT_collect_pulse4_440minET_rep2 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 112


title: Continuous_2nM GnRH_2hT_collect_pulse4_440minET_rep4 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 114


title: No pulse_0nM GnRH_0hT_collect_pulse3_400minET_rep3 [112015data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 3


title: Continuous_2nM GnRH_2hT_collect_pulse4_440minET_rep3 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 113


title: Continuous_2nM GnRH_2hT_collect_pulse4_460minET_rep5 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 120


title: Continuous_2nM GnRH_2hT_collect_pulse4_420minET_rep4 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 109


title: Continuous_2nM GnRH_2hT_collect_pulse4_420minET_rep3 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 108


title: Continuous_2nM GnRH_2hT_collect_pulse4_420minET_rep2 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 107


title: Continuous_2nM GnRH_2hT_collect_pulse3_400minET_rep5 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 105


title: Continuous_2nM GnRH_2hT_collect_pulse4_420minET_rep5 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 110


title: Continuous_2nM GnRH_2hT_collect_pulse3_400minET_rep4 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 104


title: Continuous_2nM GnRH_2hT_collect_pulse4_440minET_rep1 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 111


title: Continuous_2nM GnRH_2hT_collect_pulse3_400minET_rep3 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 103


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_480minET_rep5 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 100


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_460minET_rep4 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 94


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_460minET_rep2 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 92


title: Continuous_2nM GnRH_2hT_collect_pulse3_400minET_rep2 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 102


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_480minET_rep4 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 99


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_480minET_rep2 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 97


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_460minET_rep3 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 93


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_480minET_rep3 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 98


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_440minET_rep5 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 90


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_440minET_rep4 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 89


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_440minET_rep2 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 87


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_460minET_rep5 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 95


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_460minET_rep1 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 91


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_440minET_rep1 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 86


title: Continuous_2nM GnRH_2hT_collect_pulse4_420minET_rep1 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 106


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep5 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 85


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep4 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 84


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_480minET_rep1 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 96


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep3 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 83


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep2 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 82


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep1 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 81


title: Continuous_2nM GnRH_2hT_collect_pulse3_400minET_rep1 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 101


title: 10min pulse_2nM GnRH_2hT_collect_pulse3_400minET_rep4 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 79


title: 10min pulse_2nM GnRH_2hT_collect_pulse3_400minET_rep5 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 80


title: 10min pulse_2nM GnRH_2hT_collect_pulse3_400minET_rep3 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 78


title: 10min pulse_2nM GnRH_2hT_collect_pulse4_440minET_rep3 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 88


title: 10min pulse_2nM GnRH_2hT_collect_pulse3_400minET_rep1 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 76


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_480minET_rep5 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 75


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_480minET_rep4 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 74


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_460minET_rep3 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 68


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_460minET_rep4 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 69


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_480minET_rep3 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 73


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_460minET_rep1 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 66


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_460minET_rep2 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 67


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_480minET_rep2 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 72


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_440minET_rep5 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 65


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep5 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 60


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_440minET_rep3 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 63


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_440minET_rep4 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 64


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_460minET_rep5 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 70


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_440minET_rep2 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 62


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep3 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 58


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep4 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 59


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep2 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 57


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_420minET_rep1 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 56


title: 10min pulse_2nM GnRH_2hT_collect_pulse3_400minET_rep2 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 77


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_480minET_rep1 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 71


title: 5min pulse_2nM GnRH_2hT_collect_pulse3_400minET_rep5 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 55


title: 5min pulse_2nM GnRH_2hT_collect_pulse3_400minET_rep4 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 54


title: 5min pulse_2nM GnRH_2hT_collect_pulse3_400minET_rep1 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 51


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_480minET_rep4 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 49


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_480minET_rep5 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 50


title: 5min pulse_2nM GnRH_2hT_collect_pulse3_400minET_rep2 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 52


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_480minET_rep2 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 47


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_440minET_rep1 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 61


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_480minET_rep3 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 48


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_460minET_rep3 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 43


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_480minET_rep1 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 46


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_460minET_rep5 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 45


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_460minET_rep4 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 44


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_460minET_rep2 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 42


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_440minET_rep5 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 40


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_460minET_rep1 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 41


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_440minET_rep2 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 37


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_440minET_rep3 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 38


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_440minET_rep1 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 36


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_420minET_rep5 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 35


title: 5min pulse_2nM GnRH_2hT_collect_pulse3_400minET_rep3 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 53


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_420minET_rep4 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 34


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_420minET_rep1 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 31


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_420minET_rep2 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 32


title: 5min pulse_0nM GnRH_2hT_collect_pulse3_400minET_rep5 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 30


title: 5min pulse_0nM GnRH_2hT_collect_pulse3_400minET_rep4 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 29


title: 5min pulse_0nM GnRH_2hT_collect_pulse3_400minET_rep2 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 27


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_440minET_rep4 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 39


title: No pulse_0nM GnRH_0hT_collect_pulse4_480minET_rep5 [111615data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 25


title: 5min pulse_0nM GnRH_2hT_collect_pulse3_400minET_rep3 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 28


title: 5min pulse_0nM GnRH_2hT_collect_pulse4_420minET_rep3 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 33


title: No pulse_0nM GnRH_0hT_collect_pulse4_460minET_rep5 [111615data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 20


title: No pulse_0nM GnRH_0hT_collect_pulse4_480minET_rep1 [111615data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 21


title: No pulse_0nM GnRH_0hT_collect_pulse4_480minET_rep3 [111615data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 23


title: No pulse_0nM GnRH_0hT_collect_pulse4_460minET_rep4 [111615data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 19


title: 5min pulse_0nM GnRH_2hT_collect_pulse3_400minET_rep1 [111615data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 26


title: No pulse_0nM GnRH_0hT_collect_pulse4_480minET_rep2 [111615data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 22


title: No pulse_0nM GnRH_0hT_collect_pulse4_480minET_rep4 [111615data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 24


title: No pulse_0nM GnRH_0hT_collect_pulse4_460minET_rep3 [111615data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 18


title: No pulse_0nM GnRH_0hT_collect_pulse4_440minET_rep3 [111615data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 13


title: No pulse_0nM GnRH_0hT_collect_pulse4_440minET_rep5 [111615data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 15


title: No pulse_0nM GnRH_0hT_collect_pulse4_420minET_rep4 [111615data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 9


title: No pulse_0nM GnRH_0hT_collect_pulse4_460minET_rep1 [111615data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 16


title: No pulse_0nM GnRH_0hT_collect_pulse4_420minET_rep3 [111615data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 8


title: No pulse_0nM GnRH_0hT_collect_pulse4_440minET_rep1 [111615data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 11


title: No pulse_0nM GnRH_0hT_collect_pulse3_400minET_rep5 [111615data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 5


title: No pulse_0nM GnRH_0hT_collect_pulse3_400minET_rep4 [111615data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 4


title: No pulse_0nM GnRH_0hT_collect_pulse4_440minET_rep4 [111615data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 14


title: No pulse_0nM GnRH_0hT_collect_pulse4_460minET_rep2 [111615data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 17


title: No pulse_0nM GnRH_0hT_collect_pulse4_440minET_rep2 [111615data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 12


title: No pulse_0nM GnRH_0hT_collect_pulse3_400minET_rep2 [111615data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 2


title: No pulse_0nM GnRH_0hT_collect_pulse4_420minET_rep1 [111615data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 6


title: No pulse_0nM GnRH_0hT_collect_pulse4_420minET_rep5 [111615data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 10


title: No pulse_0nM GnRH_0hT_collect_pulse3_400minET_rep3 [111615data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 3


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_520minET_rep3 [111015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 57


title: No pulse_0nM GnRH_0hT_collect_pulse4_420minET_rep2 [111615data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 7


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_500minET_rep2 [111015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 53


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_520minET_rep1 [111015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 55


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_500minET_rep1 [111015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 52


title: No pulse_0nM GnRH_0hT_collect_pulse3_400minET_rep1 [111615data]

description:
Control; each biological sample represents 3 qPCR assay replicates.
SAMPLE 1


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_480minET_rep2 [111015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 50


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_500minET_rep3 [111015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 54


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_480minET_rep3 [111015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 51


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_480minET_rep1 [111015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 49


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_520minET_rep2 [111015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 56


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_460minET_rep2 [111015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 47


title: 5min pulse_2nM GnRH_2hT_collect_pulse4_460minET_rep1 [111015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 46


title: 5min pulse_2nM GnRH_2hT_collect_pulse3_400minET_rep2 [111015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 38


title: 5min pulse_2nM GnRH_2hT_collect_pulse3_440minET_rep1 [111015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 43


title: 5min pulse_2nM GnRH_2hT_collect_pulse3_420minET_rep2 [111015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 41


title: 5min pulse_2nM GnRH_2hT_collect_pulse3_420minET_rep1 [111015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 40


title: 5min pulse_2nM GnRH_2hT_collect_pulse3_440minET_rep2 [111015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 44


title: 5min pulse_2nM GnRH_2hT_collect_pulse3_440minET_rep3 [111015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 45


title: 5min pulse_2nM GnRH_2hT_collect_pulse3_400minET_rep1 [111015data]

description:
Test; each biological sample represents 3 qPCR assay replicates.
SAMPLE 37