title: SOCS3 Cre minus B

status: Public on Dec 12 2007

source_name: liver

label_protocol: Total RNA (2 ug) was labeled and hybridized to the Mouse Genome 430 2.0 arrays and hybridized according to manufacurer’s instructions.

extract_protocol: Total RNA was isolated from whole liver tissue harvested 18h after PH, as described in Supplemental materials and methods.

treatment_protocol: Hepatocyte-specific excision of the Socs3 gene was achieved by breeding Socs3 fl/fl mice with mice expressing the Cre recombinase transgene under control of the albumin promoter (Alb-Cre+), yielding Socs3 h-KO mice (SOCS3 Cre plus). Socs3 fl/fl, Alb-Cre- littermates were used as controls for all experiments, and are referred to as SOCS3 Cre minus. All mice (C57BL/6) were free of Helicobacter species, housed in a specific pathogen free facility with 12-h light/dark cycles with free access to standard food and water. 2/3 partial hepatectomies (PH) were performed as previously described.


title: X1A.1_A28CR6_205PR RAT WAT

status: Public on Oct 23 2007

type: RNA

city: Seattle

first: James

middle: William

last: MacDonald

state: WA

address: 4225 Roosevelt Way NE

department: Environmental and Occupational Health Sciences

country: USA

institute: University of Washington

hyb_protocol: The standard hybridization protocol was used as recommended by Affymterix.

scan_protocol: GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.

data_processing: GeneTraffic version 3.2-11 GCRMA normalization

label: biotin

molecule: total RNA

organism: Rattus norvegicus

source_name: white adipose tissue

label_protocol: Total RNA (2 ug) was labeled and hybridized to the RAE230 2.0 arrays and hybridized according to manufacurer’s instructions.

extract_protocol: Total RNA was extracted from frozen tissue samples by homogenization in Trizol® (Life Technologies, Grand Island, NY) and chloroform extraction followed by purification on an RNeasy® Mini column (Qiagen, Valencia, CA).

treatment_protocol: Tissues (n=5-7 animals per group) were obtained from the NIH-NIA aging rodent tissue bank. According to supplier records, animals were housed in a specific pathogen free barrier facility under contract with BioReliance. AL animals were housed 3 per cage and CR animals were housed singly. CR (60% of AL) was initiated at 4 months of age with a switch from the NIH 31 to NIH fortified diet. F344 male rats were sacrificed at 4 months (4AL) or 28 months of age (28AL, 28CR). Animals with gross tumor pathologies were excluded from the study. All animals were euthanized by CO2 asphixiation and tissues were frozen in liquid nitrogen then stored at –80°C. Subcutaneous WAT was collected from the abdominal region. Recorded body weights differed dramatically between the 3 groups (4AL = 253±8 g, 28AL = 382±20 g, 28CR = 288±7 g) and heart weight as a fraction of body weight was not significantly different between the groups (data not shown). The apical ~1/3 of the heart was homogenized for the expression studies. Sections from the cut face were stained with haematoxylin and eosin to confirm expected aging-associated pathology changes.


title: X18A.1_A4AL19_205PR RAT WAT

description: According to supplier records, animals were housed in a specific pathogen free barrier facility under contract with BioReliance. AL animals were housed 3 per cage and CR animals were housed singly. CR (60% of AL) was initiated at 4 months of age with a switch from the NIH 31 to NIH fortified diet. F344 male rats were sacrificed at 4 months (4AL) or 28 months of age (28AL, 28CR). Animals with gross tumor pathologies were excluded from the study. All animals were euthanized by CO2 asphixiation and tissues were frozen in liquid nitrogen then stored at –80°C. Subcutaneous WAT was collected from the abdominal region. Recorded body weights differed dramatically between the 3 groups (4AL = 253±8 g, 28AL = 382±20 g, 28CR = 288±7 g) and heart weight as a fraction of body weight was not significantly different between the groups (data not shown). The apical ~1/3 of the heart was homogenized for the expression studies. Sections from the cut face were stained with haematoxylin and eosin to confirm expected aging-associated pathology changes.

tag: Tissues (n=5-7 animals per group) were obtained from the NIH-NIA aging rodent tissue bank.


title: zebrafish brain4 low

status: Public on Jul 06 2009

description: Wild type zebrafish (Danio rerio, ABC strain) were obtained from the University of Oregon, Zebrafish International Resource Center (Eugene, OR, USA) and maintained at the Northwest Fisheries Science Center in a self-contained re-circulating aquaculture ZMOD system (Marine Biotech Inc.).  The facility is operated according to standard procedures for zebrafish husbandry and research (Westerfield 1995).  Fish were maintained at 26°C, kept on a 14:10 h light-dark cycle, and fed daily with purified ground salmon feed and brine shrimp. Fish were not fed on the morning of each exposure experiment.

data_processing: Bioconductor GCRMA normalization log2

organism: Danio rerio

source_name: brain

label_protocol: Total RNA (2 ug) was labeled and hybridized to the Zebrafish Genome Arrays and hybridized according to manufacurer’s instructions.

extract_protocol: Total RNA was extracted from individual snap frozen zebrafish brains using a standard TRIzol procedure with the inclusion of 1 µl RNAse inhibitor/sample.

treatment_protocol: To quantify DA-induced neurobehavioral excitotoxicity in zebrafish, adult fish were exposed to DA via intracoelomic (IC) injection at several doses.  For injection, fish were netted individually, placed into Ziplock bags and injected through the bag at a point midway between the pectoral fins and the anus.  Contents (10 ml) were slowly injected into the IC cavity.  IC injections consisted of a vehicle control (phosphate buffered saline (PBS) only) or DA dissolved in PBS.  Eight exposure doses (0.21 ± 0.01, 0.25 ± 0.03, 0.32 ± 0.04, 0.47 ± 0.07, 0.82 ± 0.09, 1.2 ± 0.15, 1.6 ± 0.1, and 3.2 ± 0.5 µg DA/g body weight; n = 4 fish per treatment) were used.  After injection, four fish from each treatment were placed in 1-liter clear plastic tanks and observed for 6 h.  Behavioral toxicity was quantified by the presence or absence of circle or spiral swimming.  Time to the onset of excitotoxicity and the number of fish affected were recorded for each treatment.  Percentages of fish affected at each dose were used to generate a dose-response curve and an EC50 for behavioral toxicity using Graphpad Prism® software (San Diego, CA).  All DA exposure doses were confirmed by HPLC-UV analysis (see HPLC verification of DA exposure doses below). All animal studies were carried out under approved IACUC protocols at the University of Washington.


title: X9A.1_A28CR7_205PR RAT WAT


title: X4A.1_A28AL6_205PR RAT WAT


title: zebrafish brain2 control

tag: Wild type zebrafish (Danio rerio, ABC strain) were obtained from the University of Oregon, Zebrafish International Resource Center (Eugene, OR, USA) and maintained at the Northwest Fisheries Science Center in a self-contained re-circulating aquaculture ZMOD system (Marine Biotech Inc.).  The facility is operated according to standard procedures for zebrafish husbandry and research (Westerfield 1995).  Fish were maintained at 26°C, kept on a 14:10 h light-dark cycle, and fed daily with purified ground salmon feed and brine shrimp. Fish were not fed on the morning of each exposure experiment.


title: X14A.1_A4AL17_205PR RAT WAT


title: X13A.1_A28AL11_205PR RAT WAT


title: zebrafish brain3 control


title: X17A.1_A28CR8_205PR RAT WAT


title: X15A.1_A4AL18_205PR RAT WAT


title: X10A.1_A28CR9_205PR RAT WAT


title: X12A.1_A28AL9_205PR RAT WAT


title: CR.28.9 RAT HEART

source_name: heart


title: X16A.1_A4AL22_205PR RAT WAT


title: CR.28.8 RAT HEART


title: X11A.1_A28CR12_205PR RAT WAT


title: CR.28.6 RAT HEART


title: AL.4.20 RAT HEART


title: CR.28.11 RAT HEART


title: CR.28.10 RAT HEART


title: AL.4.22 RAT HEART


title: AL.4.21 RAT HEART


title: CR.28.7 RAT HEART


title: CR.28.12 RAT HEART


title: AL.28.9 RAT HEART


title: AL.4.23 RAT HEART


title: AL.4.18 RAT HEART


title: AL.4.17 RAT HEART


title: AL.28.10 RAT HEART


title: AL.28.8 RAT HEART


title: AL.28.6 RAT HEART


title: AL.28.7 RAT HEART


title: AL.28.12 RAT HEART


title: AL.28.11 RAT HEART


title: MPI-227

status: Public on Jun 07 2006

city: Berlin

first: Michael

last: Hummel

address: Hindenburgdamm 30

department: Institute of Pathology

country: Germany

institute: Universitätsmedizin Berlin, Campus Benjamin Franklin

description: Mature aggressive B-cell lymphoma

data_processing: Probe level normalization was done using the calibration and variance stabilization method by Huber et al. (Bioinformatics 2002, PMID: 12169536).  Probe-set summarization was performed using the median polish method on the normalized data (Irrizary et al. Nucleic Acids Research 2003, PMID: 12582260).

label: Biotin

organism: Homo sapiens

source_name: Frozen tissue

tag: Relapse

value: no

tag: Age

tag: Sex

value: female

value: aggressive B-NHL.unclassifiable

value: present

value: absent

value: MYC-negative

value: IV

tag: Lesions

value: alive

tag: Radiotherapy

tag: RITUXIMAB

value: CR

tag: Chemotherapy

value: CHOP-like

value: non-mBL

value: GCB

value: Test


title: MPI-229

description: Mature aggressive B-cell lymphoma

value: DLBCL

value: non-IG-MYC

value: nodal + extranodal

value: CRu

value: Training


title: MPI-198

description: Mature aggressive B-cell lymphoma

value: no

value: female

value: III

value: extranodal

value: dead


title: MPI-212

description: Mature aggressive B-cell lymphoma

value: male

value: present

value: absent

value: MYC-negative

value: PR


title: MPI-203

description: Mature aggressive B-cell lymphoma

value: nodal

value: CHOP-like

value: non-mBL

value: GCB


title: MPI-237

description: Mature aggressive B-cell lymphoma

value: DLBCL

value: IG-MYC

value: alive

value: other


title: MPI-241

description: Mature aggressive B-cell lymphoma

value: no

value: female

value: aggressive B-NHL.unclassifiable

value: IV

value: nodal + extranodal

value: CR

value: Test


title: MPI-221

description: Mature aggressive B-cell lymphoma

value: unk

value: male

value: present

value: absent

value: MYC-negative

value: Training


title: MPI-255

description: Mature aggressive B-cell lymphoma

value: DLBCL

value: non-mBL

value: GCB


title: MPI-235

description: Mature aggressive B-cell lymphoma


title: MPI-207

description: Mature aggressive B-cell lymphoma

value: II

value: nodal

value: alive

value: yes

value: CHOP-like


title: MPI-226

description: Mature aggressive B-cell lymphoma

value: BL-leukemia

value: no


title: MPI-231

description: Mature aggressive B-cell lymphoma

value: female


title: MPI-223b

description: Mature aggressive B-cell lymphoma

value: III

value: PR

value: Test


title: MPI-228

description: Mature aggressive B-cell lymphoma

value: male

value: aggressive B-NHL.unclassifiable

value: present

value: absent

value: IV

value: CR


title: MPI-176

description: Mature aggressive B-cell lymphoma

value: DLBCL

value: IG-MYC

value: dead

value: Progress

value: non-mBL

value: GCB


title: MPI-233

description: Mature aggressive B-cell lymphoma

value: no

value: MYC-negative

value: II

value: nodal

value: alive

value: ALL-like

value: Training


title: MPI-153

description: Mature aggressive B-cell lymphoma

value: female

value: present


title: MPI-202

description: Mature aggressive B-cell lymphoma

value: male

value: aggressive B-NHL.unclassifiable

value: absent

value: IV

value: nodal + extranodal

value: other

value: Test


title: MPI-162

description: Mature aggressive B-cell lymphoma

value: DLBCL

value: I

value: extranodal

value: dead

value: Progress

value: CHOP-like

value: intermediate

value: GCB


title: MPI-142

description: Mature aggressive B-cell lymphoma

value: no

value: female

value: MYC-negative

value: nodal

value: non-mBL

value: Training


title: MPI-150

description: Mature aggressive B-cell lymphoma

value: male

value: present

value: absent

value: III

value: alive

value: yes

value: PR


title: MPI-163

description: Mature aggressive B-cell lymphoma

value: DLBCL

value: IV

value: nodal + extranodal

value: CR


title: MPI-209

description: Mature aggressive B-cell lymphoma

value: unk

value: aggressive B-NHL.unclassifiable

value: GCB

value: no


title: MPI-154

description: Mature aggressive B-cell lymphoma

value: female

value: I

value: nodal

value: CHOP-like

value: non-mBL

value: Test


title: MPI-166

description: Mature aggressive B-cell lymphoma

value: male

value: present

value: absent

value: MYC-negative

value: III

value: dead

value: CRu


title: MPI-173

description: Mature aggressive B-cell lymphoma

value: unk

value: atypical BL

value: Training


title: MPI-136

description: Mature aggressive B-cell lymphoma

value: DLBCL

value: GCB

value: no


title: MPI-189

description: Mature aggressive B-cell lymphoma

value: IG-MYC


title: MPI-134

description: Mature aggressive B-cell lymphoma

value: female


title: MPI-127

description: Mature aggressive B-cell lymphoma

value: non-mBL


title: MPI-128

description: Mature aggressive B-cell lymphoma

value: Test


title: MPI-129

description: Mature aggressive B-cell lymphoma

value: male

value: II

value: alive

value: yes

value: CHOP-like


title: MPI-120

description: Mature aggressive B-cell lymphoma

value: present

value: absent

value: intermediate


title: MPI-109

description: Mature aggressive B-cell lymphoma

value: MYC-negative

value: III

value: CR

value: other


title: MPI-119

description: Mature aggressive B-cell lymphoma

value: no

value: DLBCL

value: I

value: extranodal

value: GCB


title: MPI-105

description: Mature aggressive B-cell lymphoma

value: female

value: nodal

value: CRu

value: non-mBL

value: Test


title: MPI-113

description: Mature aggressive B-cell lymphoma

value: unk

value: male

value: present

value: Training


title: MPI-104

description: Mature aggressive B-cell lymphoma

value: absent

value: non-IG-MYC

value: IV

value: dead

value: yes

value: Progress

value: CHOP-like

value: intermediate


title: MPI-092

description: Mature aggressive B-cell lymphoma

value: DLBCL

value: MYC-negative

value: GCB

value: no


title: MPI-096

description: Mature aggressive B-cell lymphoma

value: II

value: nodal

value: alive

value: NC

value: non-mBL

value: Test


title: MPI-053

description: Mature aggressive B-cell lymphoma

value: female

value: present

value: absent

value: III

value: CR


title: MPI-122

description: Mature aggressive B-cell lymphoma

value: male

value: CHOP-like


title: MPI-022

description: Mature aggressive B-cell lymphoma

value: DLBCL

value: dead

value: CRu

value: Training


title: MPI-046

description: Mature aggressive B-cell lymphoma

value: no

value: non-IG-MYC

value: intermediate

value: GCB


title: MPI-025

description: Mature aggressive B-cell lymphoma

value: MYC-negative

value: II

value: alive

value: non-mBL


title: MPI-036

description: Mature aggressive B-cell lymphoma

value: female

value: present

value: absent

value: IG-MYC

value: I

value: extranodal

value: yes

value: Progress

value: other


title: MPI-024

description: Mature aggressive B-cell lymphoma

value: no

value: DLBCL

value: nodal

value: dead

value: CR

value: CHOP-like

value: Test


title: MPI-039

description: Mature aggressive B-cell lymphoma

value: MYC-negative

value: III

value: nodal + extranodal

value: non-mBL

value: GCB


title: MPI-010

description: Mature aggressive B-cell lymphoma

value: unk

value: male

value: present

value: absent

value: Training


title: MPI-030

description: Mature aggressive B-cell lymphoma

value: no

value: Progress


title: New El-Ashry 010.CEL

status: Public on Apr 06 2006

city: Houston

first: Chad

last: Creighton

state: TX

address: One Baylor Plaza, Mail Stop: BCM305

department: Biostatistics, Ducan Cancer Center

institute: Baylor College of Medicine

description: MCF-7 co-transfected with ligand-inducible EGFR

data_processing: RMAExpress version 0.3

tag: MCF-7 breast cancer cells


title: El-Ashry 005.CEL

description: MCF-7 co-transfected with constituitively active Raf


title: New El-Ashry 012.CEL


title: CP 5 hr treatment 5.2.CP5.1479.1480 2.3000001479B.spt.geo.txt

status: Public on Feb 24 2004

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 5.2.CP5.1479.14802.3000001479B.spt.geo.txt

organism: Mus musculus

source_name: treated embryos

source_name: control embryos


title: CP 5 hr treatment 3.2.CP5.103945.111225 4.2000111225B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 3.2.CP5.103945.1112254.2000111225B.spt.geo.txt


title: CP 5 hr treatment 5.2.CP5.1479.1480 3.3000001480.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 5.2.CP5.1479.14803.3000001480.spt.geo.txt


title: New El-Ashry 004.CEL


title: CP 5 hr treatment 2.2.CP5.103933.103934 4.2000103934B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 2.2.CP5.103933.1039344.2000103934B.spt.geo.txt


title: El-Ashry 004.CEL


title: CP 5 hr treatment 1.2.CP5.103929.103930 4.2000103930B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 1.2.CP5.103929.1039304.2000103930B.spt.geo.txt


title: CP 5 hr treatment 4.2.CP5.1475.1476 3.3000001476.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 4.2.CP5.1475.14763.3000001476.spt.geo.txt


title: CP 5 hr treatment 4.2.CP5.1475.1476 4.3000001476B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 4.2.CP5.1475.14764.3000001476B.spt.geo.txt


title: New El-Ashry 006.CEL


title: CP 5 hr treatment 5.2.CP5.1479.1480 4.3000001480B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 5.2.CP5.1479.14804.3000001480B.spt.geo.txt


title: CP 5 hr treatment 3.2.CP5.103945.111225 3.2000111225.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 3.2.CP5.103945.1112253.2000111225.spt.geo.txt


title: CP 5 hr treatment 2.2.CP5.103933.103934 3.2000103934.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 2.2.CP5.103933.1039343.2000103934.spt.geo.txt


title: CP 5 hr treatment 1.2.CP5.103929.103930 2.2000103929B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 1.2.CP5.103929.1039302.2000103929B.spt.geo.txt


title: CP 5 hr treatment 2.2.CP5.103933.103934 2.2000103933B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 2.2.CP5.103933.1039342.2000103933B.spt.geo.txt


title: CP 5 hr treatment 3.2.CP5.103945.111225 2.2000103945B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 3.2.CP5.103945.1112252.2000103945B.spt.geo.txt


title: CP 5 hr treatment 1.2.CP5.103929.103930 3.2000103930.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 1.2.CP5.103929.1039303.2000103930.spt.geo.txt


title: CP 5 hr treatment 5.2.CP5.1479.1480 1.3000001479.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 5.2.CP5.1479.14801.3000001479.spt.geo.txt


title: CP 5 hr treatment 4.2.CP5.1475.1476 1.3000001475.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 4.2.CP5.1475.14761.3000001475.spt.geo.txt


title: CP 5 hr treatment 2.2.CP5.103933.103934 1.2000103933.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 2.2.CP5.103933.1039341.2000103933.spt.geo.txt


title: CP 5 hr treatment 1.2.CP5.103929.103930 1.2000103929.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 1.2.CP5.103929.1039301.2000103929.spt.geo.txt


title: CP 5 hr treatment 3.2.CP5.103945.111225 1.2000103945.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 3.2.CP5.103945.1112251.2000103945.spt.geo.txt


title: CP 5 hr treatment 4.2.CP5.1475.1476 2.3000001475B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 4.2.CP5.1475.14762.3000001475B.spt.geo.txt


title: Heat Shock 5 hr treatment 3.2.HS5.111247.111248 4.2000111248B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 3.2.HS5.111247.1112484.2000111248B.spt.geo.txt


title: Heat Shock 5 hr treatment 2.2.HS5.111250.111253 4.2000111253B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 2.2.HS5.111250.1112534.2000111253B.spt.geo.txt


title: Heat Shock 5 hr treatment 2.2.HS5.111250.111253 3.2000111253.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 2.2.HS5.111250.1112533.2000111253.spt.geo.txt


title: Heat Shock 5 hr treatment 4.2.HS5.111234.111235 2.2000111234B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 4.2.HS5.111234.1112352.2000111234B.spt.geo.txt


title: Heat Shock 5 hr treatment 3.2.HS5.111247.111248 1.2000111247.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 3.2.HS5.111247.1112481.2000111247.spt.geo.txt


title: Heat Shock 5 hr treatment 3.2.HS5.111247.111248 3.2000111248.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 3.2.HS5.111247.1112483.2000111248.spt.geo.txt


title: Heat Shock 5 hr treatment 2.2.HS5.111250.111253 1.2000111250.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 2.2.HS5.111250.1112531.2000111250.spt.geo.txt


title: Heat Shock 5 hr treatment 4.2.HS5.111234.111235 4.2000111235B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 4.2.HS5.111234.1112354.2000111235B.spt.geo.txt


title: Heat Shock 5 hr treatment 2.2.HS5.111250.111253 2.2000111250B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 2.2.HS5.111250.1112532.2000111250B.spt.geo.txt


title: Heat Shock 1 hr treatment 2.2.HS1.111230.111231 4.2000111231B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 2.2.HS1.111230.1112314.2000111231B.spt.geo.txt


title: Heat Shock 5 hr treatment 4.2.HS5.111234.111235 3.2000111235.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 4.2.HS5.111234.1112353.2000111235.spt.geo.txt


title: Heat Shock 5 hr treatment 3.2.HS5.111247.111248 2.2000111247B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 3.2.HS5.111247.1112482.2000111247B.spt.geo.txt


title: Heat Shock 1 hr treatment 1.2.HS1.111226.111227 4.2000111227B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 1.2.HS1.111226.1112274.2000111227B.spt.geo.txt


title: Heat Shock 1 hr treatment 3.2.HS1.111228.111229 3.2000111229.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 3.2.HS1.111228.1112293.2000111229.spt.geo.txt


title: Heat Shock 1 hr treatment 1.2.HS1.111226.111227 3.2000111227.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 1.2.HS1.111226.1112273.2000111227.spt.geo.txt


title: CP 1 hr treatment 1.2.CP1.1484.1485 4.3000001485B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 1.2.CP1.1484.14854.3000001485B.spt.geo.txt


title: Heat Shock 1 hr treatment 3.2.HS1.111228.111229 2.2000111228B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 3.2.HS1.111228.1112292.2000111228B.spt.geo.txt


title: Heat Shock 1 hr treatment 1.2.HS1.111226.111227 2.2000111226B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 1.2.HS1.111226.1112272.2000111226B.spt.geo.txt


title: CP 1 hr treatment 3.2.CP1.1481.1482 4.3000001482B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 3.2.CP1.1481.14824.3000001482B.spt.geo.txt


title: Heat Shock 5 hr treatment 4.2.HS5.111234.111235 1.2000111234.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 4.2.HS5.111234.1112351.2000111234.spt.geo.txt


title: Heat Shock 1 hr treatment 2.2.HS1.111230.111231 2.2000111230B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 2.2.HS1.111230.1112312.2000111230B.spt.geo.txt


title: Heat Shock 1 hr treatment 3.2.HS1.111228.111229 1.2000111228.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 3.2.HS1.111228.1112291.2000111228.spt.geo.txt


title: Heat Shock 1 hr treatment 2.2.HS1.111230.111231 1.2000111230.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 2.2.HS1.111230.1112311.2000111230.spt.geo.txt


title: Heat Shock 1 hr treatment 3.2.HS1.111228.111229 4.2000111229B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 3.2.HS1.111228.1112294.2000111229B.spt.geo.txt


title: Heat Shock 1 hr treatment 1.2.HS1.111226.111227 1.2000111226.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 1.2.HS1.111226.1112271.2000111226.spt.geo.txt


title: Heat Shock 1 hr treatment 2.2.HS1.111230.111231 3.2000111231.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 2.2.HS1.111230.1112313.2000111231.spt.geo.txt


title: CP 1 hr treatment 2.2.CP1.1473.1474 4.3000001474B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 2.2.CP1.1473.14744.3000001474B.spt.geo.txt


title: CP 1 hr treatment 3.2.CP1.1481.1482 3.3000001482.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 3.2.CP1.1481.14823.3000001482.spt.geo.txt


title: CP 1 hr treatment 2.2.CP1.1473.1474 3.3000001474.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 2.2.CP1.1473.14743.3000001474.spt.geo.txt


title: CP 1 hr treatment 1.2.CP1.1484.1485 3.3000001485.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 1.2.CP1.1484.14853.3000001485.spt.geo.txt


title: CP 1 hr treatment 3.2.CP1.1481.1482 1.3000001481.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 3.2.CP1.1481.14821.3000001481.spt.geo.txt


title: CP 1 hr treatment 2.2.CP1.1473.1474 1.3000001473.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 2.2.CP1.1473.14741.3000001473.spt.geo.txt


title: CP 5 hr treatment 5.1.CP5.32954.32955 4.3000032955B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 5.1.CP5.32954.329554.3000032955B.spt.geo.txt


title: CP 5 hr treatment 2.1.CP5.14098,114099 3.2000114099.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 2.1.CP5.14098,1140993.2000114099.spt.geo.txt


title: CP 1 hr treatment 1.2.CP1.1484.1485 2.3000001484B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 1.2.CP1.1484.14852.3000001484B.spt.geo.txt


title: CP 1 hr treatment 3.2.CP1.1481.1482 2.3000001481B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 3.2.CP1.1481.14822.3000001481B.spt.geo.txt


title: CP 5 hr treatment 4.1.CP5.32952.32953 4.3000032953B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 4.1.CP5.32952.329534.3000032953B.spt.geo.txt


title: CP 5 hr treatment 1.1.CP5.114081.114082 3.2000114082.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 1.1.CP5.114081.1140823.2000114082.spt.geo.txt


title: CP 5 hr treatment 4.1.CP5.32952.32953 3.3000032953.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 4.1.CP5.32952.329533.3000032953.spt.geo.txt


title: CP 1 hr treatment 2.2.CP1.1473.1474 2.3000001473B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 2.2.CP1.1473.14742.3000001473B.spt.geo.txt


title: CP 1 hr treatment 1.2.CP1.1484.1485 1.3000001484.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 1.2.CP1.1484.14851.3000001484.spt.geo.txt


title: CP 5 hr treatment 3.1.CP5.32947.32948 3.3000032948.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 3.1.CP5.32947.329483.3000032948.spt.geo.txt


title: CP 5 hr treatment 2.1.CP5.14098,114099 2.2000114098B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 2.1.CP5.14098,1140992.2000114098B.spt.geo.txt


title: CP 5 hr treatment 5.1.CP5.32954.32955 3.3000032955.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 5.1.CP5.32954.329553.3000032955.spt.geo.txt


title: CP 5 hr treatment 3.1.CP5.32947.32948 4.3000032948B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 3.1.CP5.32947.329484.3000032948B.spt.geo.txt


title: CP 5 hr treatment 5.1.CP5.32954.32955 2.3000032954B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 5.1.CP5.32954.329552.3000032954B.spt.geo.txt


title: CP 5 hr treatment 1.1.CP5.114081.114082 4.2000114082B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 1.1.CP5.114081.1140824.2000114082B.spt.geo.txt


title: CP 5 hr treatment 5.1.CP5.32954.32955 1.3000032954.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 5.1.CP5.32954.329551.3000032954.spt.geo.txt


title: CP 5 hr treatment 4.1.CP5.32952.32953 1.3000032952.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 4.1.CP5.32952.329531.3000032952.spt.geo.txt


title: CP 5 hr treatment 3.1.CP5.32947.32948 1.3000032947.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 3.1.CP5.32947.329481.3000032947.spt.geo.txt


title: CP 5 hr treatment 2.1.CP5.14098,114099 1.2000114098.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 2.1.CP5.14098,1140991.2000114098.spt.geo.txt


title: CP 5 hr treatment 1.1.CP5.114081.114082 2.2000114081B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 1.1.CP5.114081.1140822.2000114081B.spt.geo.txt


title: Heat Shock 5 hr treatment 4.1.HS5.118041.118042 4.2000118042B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 4.1.HS5.118041.1180424.2000118042B.spt.geo.txt


title: CP 5 hr treatment 3.1.CP5.32947.32948 2.3000032947B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 3.1.CP5.32947.329482.3000032947B.spt.geo.txt


title: Heat Shock 5 hr treatment 2.1.HS5.110349.110350 3.2000110350.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 2.1.HS5.110349.1103503.2000110350.spt.geo.txt


title: Heat Shock 5 hr treatment 4.1.HS5.118041.118042 3.2000118042.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 4.1.HS5.118041.1180423.2000118042.spt.geo.txt


title: Heat Shock 5 hr treatment 3.1.HS5.110348.110255 4.2000110255B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 3.1.HS5.110348.1102554.2000110255B.spt.geo.txt


title: Heat Shock 5 hr treatment 2.1.HS5.110349.110350 4.2000110350B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 2.1.HS5.110349.1103504.2000110350B.spt.geo.txt


title: Heat Shock 1 hr treatment 3.1.HS1.110332.110340 4.2000111340B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 3.1.HS1.110332.1103404.2000111340B.spt.geo.txt


title: Heat Shock 5 hr treatment 3.1.HS5.110348.110255 3.2000110255.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 3.1.HS5.110348.1102553.2000110255.spt.geo.txt


title: Heat Shock 5 hr treatment 3.1.HS5.110348.110255 2.2000110348B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 3.1.HS5.110348.1102552.2000110348B.spt.geo.txt


title: Heat Shock 5 hr treatment 2.1.HS5.110349.110350 2.2000110349B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 2.1.HS5.110349.1103502.2000110349B.spt.geo.txt


title: Heat Shock 1 hr treatment 2.1.HS1.110330.110331 4.2000110331B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 2.1.HS1.110330.1103314.2000110331B.spt.geo.txt


title: Heat Shock 5 hr treatment 4.1.HS5.118041.118042 2.2000118041B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 4.1.HS5.118041.1180422.2000118041B.spt.geo.txt


title: Heat Shock 1 hr treatment 1.1.HS1.110327.110329 4.2000110329B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 1.1.HS1.110327.1103294.2000110329B.spt.geo.txt


title: Heat Shock 5 hr treatment 2.1.HS5.110349.110350 1.2000110349.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 2.1.HS5.110349.1103501.2000110349.spt.geo.txt


title: Heat Shock 1 hr treatment 3.1.HS1.110332.110340 3.2000111340.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 3.1.HS1.110332.1103403.2000111340.spt.geo.txt


title: Heat Shock 5 hr treatment 4.1.HS5.118041.118042 1.2000118041.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 4.1.HS5.118041.1180421.2000118041.spt.geo.txt


title: Heat Shock 1 hr treatment 3.1.HS1.110332.110340 2.2000110332B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 3.1.HS1.110332.1103402.2000110332B.spt.geo.txt


title: Heat Shock 5 hr treatment 3.1.HS5.110348.110255 1.2000110348.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 3.1.HS5.110348.1102551.2000110348.spt.geo.txt


title: Heat Shock 1 hr treatment 1.1.HS1.110327.110329 3.2000110329.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 1.1.HS1.110327.1103293.2000110329.spt.geo.txt


title: Heat Shock 1 hr treatment 2.1.HS1.110330.110331 2.2000110330B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 2.1.HS1.110330.1103312.2000110330B.spt.geo.txt


title: Heat Shock 1 hr treatment 2.1.HS1.110330.110331 3.2000110331.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 2.1.HS1.110330.1103313.2000110331.spt.geo.txt


title: Heat Shock 1 hr treatment 1.1.HS1.110327.110329 2.2000110327B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 1.1.HS1.110327.1103292.2000110327B.spt.geo.txt


title: Heat Shock 1 hr treatment 2.1.HS1.110330.110331 1.2000110330.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 2.1.HS1.110330.1103311.2000110330.spt.geo.txt


title: Heat Shock 1 hr treatment 1.1.HS1.110327.110329 1.2000110327.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 1.1.HS1.110327.1103291.2000110327.spt.geo.txt


title: CP 1 hr treatment 3.1.CP1.32956.32957 4.3000032957B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 3.1.CP1.32956.329574.3000032957B.spt.geo.txt


title: CP 1 hr treatment 3.1.CP1.32956.32957 3.3000032957.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 3.1.CP1.32956.329573.3000032957.spt.geo.txt


title: CP 1 hr treatment 2.1.CP1.32949.32950 4.3000032950B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 2.1.CP1.32949.329504.3000032950B.spt.geo.txt


title: Heat Shock 1 hr treatment 3.1.HS1.110332.110340 1.2000110332.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 3.1.HS1.110332.1103401.2000110332.spt.geo.txt


title: CP 1 hr treatment 2.1.CP1.32949.32950 3.3000032950.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 2.1.CP1.32949.329503.3000032950.spt.geo.txt


title: CP 1 hr treatment 1.1.CP1.32945.32946 3.3000032946.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 1.1.CP1.32945.329463.3000032946.spt.geo.txt


title: CP 5 hr treatment 4.1.CP5.32952.32953 2.3000032952B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 4.1.CP5.32952.329532.3000032952B.spt.geo.txt


title: CP 1 hr treatment 3.1.CP1.32956.32957 2.3000032956B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 3.1.CP1.32956.329572.3000032956B.spt.geo.txt


title: CP 1 hr treatment 1.1.CP1.32945.32946 1.3000032945.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 1.1.CP1.32945.329461.3000032945.spt.geo.txt


title: CP 1 hr treatment 3.1.CP1.32956.32957 1.3000032956.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 3.1.CP1.32956.329571.3000032956.spt.geo.txt


title: CP 1 hr treatment 2.1.CP1.32949.32950 2.3000032949B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 2.1.CP1.32949.329502.3000032949B.spt.geo.txt


title: CP 1 hr treatment 2.1.CP1.32949.32950 1.3000032949.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 2.1.CP1.32949.329501.3000032949.spt.geo.txt


title: CP 1 hr treatment 1.1.CP1.32945.32946 4.3000032946B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 1.1.CP1.32945.329464.3000032946B.spt.geo.txt


title: CP 1 hr treatment 1.1.CP1.32945.32946 2.3000032945B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 1.1.CP1.32945.329462.3000032945B.spt.geo.txt


title: CP 5 hr treatment 2.1.CP5.14098,114099 4.2000114099B.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 2.1.CP5.14098,1140994.2000114099B.spt.geo.txt


title: CP 5 hr treatment 1.1.CP5.114081.114082 1.2000114081.spt.geo.txt

description:
one group of embryos was cultured at 37°C (untreated control)
another group was exposed either to 43°C for 15 minutes (HS)
or 40 uM 4-hydroperoxycyclophosphamide (4CP) (a pre-activated analogue of cyclophosphamide)
After exposure to 43°C for 15 minutes
HS-treated embryos were returned to culture at 37°C and incubated for 1 or 5 hrs
4CP-treated embryos were cultured at 37°C with drug continuously for 1 or 5 hours
Lot batch = 1.1.CP5.114081.1140821.2000114081.spt.geo.txt