title: stem base genotype 160, biological rep1

status: Public on Jan 31 2018

type: RNA

city: Montpellier

first: Pascal

last: Gantet

address: Avenue Agropolis

department: IRD

country: France

institute: Université Montpellier 2

description: Gene expression data from stem bases of 22 days old plantlets

hyb_protocol: All steps of Microarray hybridization and data processing were performed according manufacturer’s instructions (Affymetrix). Equipments were provided by Affymetrix Company. Twelve μg of biotin-labelled antisens RNAs were fragmented and hybridized to GeneChip Rice Genome Arrays (Affymetrix) for 16h at 45°C and 60rpm in a Hybridization Oven 645.

scan_protocol: Arrays were washed, labeled with phycoerythrin on the Fluidic Station 450 and read with the Scanner 3000 7G.

data_processing: Data acquisition was done with the GeneChip Command Console. Array pictures were analyzed with MAS5 algorithm and RMA of the Expression Console software (Affymetrix). CHP files are available for both gene-level and exon-level analysis.

label: biotin

molecule: total RNA

organism: Oryza sativa

source_name: Stem bases from 22 days old plantlets

label_protocol: Antisense RNA strand were biotynilated according the Affymetrix (Affymetrix, CA, USA) IVT Express protocol.

growth_protocol: Dehulled and surface sterilized seeds of Oryza sativa, cv. genotypes G95 and G160, were incubated in sterile closed tubes containing sterile water during 1 day and during 2 days after water removing at 26°C under a 12h light/12h dark photoperiod. Germinated seeds were transferred in  3L pots filled with EGO 140 soil substrate (TREF,  www.Trefgroup.com) in a containment greenhouse (16-h-light/8-h-dark cycles, at 28°C to 30°C). Stem bases were harvested 22 days after sowing.

extract_protocol: Total RNA was extracted from frozen five stem bases using a RNeasy Plant Mini Kit (Quiagen) according to manufacturer’s instructions. Residual genomic DNA was removed with the RNAse-Free DNase Set (Quiagen) during RNA purification. They were quantified with a NanoQuant at 260nm wavelength and analyzed for quality on a BioAnalyzer 2100 (Agilent, CA, USA).

treatment_protocol: Stem bases were collected and immediately frozen in liquid nitrogen for RNA extraction.

tag: tissue

value: Stem base

tag: cultivar

value: indica group, genotype G160

tag: age

value: 22 days old plantlet


title: stem base genotype 95, biological rep2

value: indica group, genotype G95


title: stem base genotype 160, biological rep2


title: Sk-hep1_Growth Medium_24hr_rep1

city: Taipei City

first: WEI-JIA

last: LUO

address: Rm. 521-1, 5F., No.1, Changde St., Zhongzheng Dist.

country: Taiwan

institute: Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University.

description: Gene expression data from 24 hr GM-treated Sk-hep1 cells

hyb_protocol: Following fragmentation, 10 ug of cRNA were hybridized on Human Genome 430 2.0 GeneChip (Affymetrix). GeneChips were then washed, stained and read by Affymetrix GeneChip Scanner 3000 7G

scan_protocol: GeneChips were read by Affymetrix GeneChip Scanner 3000 7G

data_processing: The data were analyzed with GeneSpring GX 14.8 (Sillicon Genetics) and GC-RMA was used as normalization method.

organism: Homo sapiens

source_name: Sk-hep1 cell line (Homo sapiens/Human, liver/ascites, endothelial cell type, adenocarcinoma)

label_protocol: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).

growth_protocol: SK-hep1 cells were grown at 37 °C in 5% CO2-humidified air in DMEM medium that contained 10% fetal bovine serum, 100 U/ml penicillin , 100 mg/ml streptomycin  and 1% L-glutamine.

extract_protocol: Trizol extraction of total RNA was performed according to the manufacturer's instructions.

treatment_protocol: Sk-hep1 cells were treated with DMEM-based growth medium or DMEM/f12-based high-fat medium with 12 or 24 hours incubation periods. Cells were washed twice with iced PBS and then trizol (Sigma Aldrich) reagent was added.

tag: cell line

value: liver/ascites

tag: cell type

value: endothelial adenocarcinoma

tag: culture medium

value: Growth Medium

tag: medium incubation time

value: 24 hours


title: Mac-2a cell line, ALCL, ALK-

city: Münster

first: Michael

last: Grau

state: Nordrhein-Westfalen

address: Albert-Schweitzer-Campus 1

department: Faculty of Medicine

country: Germany

institute: University of Münster

hyb_protocol: Affymetrix protocol

scan_protocol: Scan performed using Scanner Affymetrix, Model 7G according to the manufacturer's recommendation.

data_processing: Raw signals were processed by Affymetrix Power Tools from Affymetrix using RMA for background correction and normalization. Gene expression was quantified by RMA using affy R package.

source_name: cell line

label_protocol: Affymetrix protocol

growth_protocol: ALCL (Karpas-299 [named as K299], SU-DHL-1, DEL, JB6, SUP-M2, all ALK+; Mac-1, Mac-2A, FE-PD, DL40, all ALK–), T cell leukemia-derived (Jurkat, KE-37, Molt-14, H9) cel lines were cultured in RPMI1640 (+ Glutamax) and 10% FBS supplemented with Pen/Strep and sodium pyruvat (100 mM).

extract_protocol: QIAGEN RNAeasy kit

treatment_protocol: for the generation of gene expression profiles, cell lines were grown under cell culture conditions that ensure optimal cell growth, no special treatment applied

value: ALCL

value: ALK-

tag: description

value: Mac-2a cell line, ALK-negative anaplastic large-cell lymphoma


title: stem base genotype 95, biological rep1


title: Sk-hep1_High Fat Medium_24hr_rep3

description: Gene expression data from 24 hr HFM-treated Sk-hep1 cells

value: High-Fat Medium


title: SU-DHL-1 cell line, ALCL, ALK+

value: ALK+

value: SU-DHL-1 cell line, ALK-positive anaplastic large-cell lymphoma


title: FE-PD cell line, ALCL, ALK-

value: FE-PD

value: FE-PD cell line, ALK-negative anaplastic large-cell lymphoma


title: Sk-hep1_Growth Medium_12hr_rep3

description: Gene expression data from 12 hr GM-treated Sk-hep1 cells

value: 12 hours


title: JB6 cell line, ALCL, ALK+

value: JB6 cell line, ALK-positive anaplastic large-cell lymphoma


title: Sk-hep1_Growth Medium_24hr_rep3


title: Sk-hep1_High Fat Medium_24hr_rep1


title: Sk-hep1_High Fat Medium_12hr_rep3

description: Gene expression data from 12 hr HFM-treated Sk-hep1 cells


title: Sk-hep1_Growth Medium_12hr_rep1


title: DEL cell line, ALCL, ALK+

value: DEL

value: DEL cell line, ALK-positive anaplastic large-cell lymphoma


title: Sk-hep1_High Fat Medium_12hr_rep1


title: Molt-14 cell line, Control

value: T-ALL

value: Molt-14 cell line, control sample


title: Sk-hep1_Growth Medium_12hr_rep2

value: liver/ascites

value: endothelial adenocarcinoma


title: Sk-hep1_High Fat Medium_24hr_rep2

value: 24 hours


title: Karpas-299 cell line, ALCL, ALK+

value: ALCL

value: Karpas-299 cell line, ALK-positive anaplastic large-cell lymphoma


title: Sk-hep1_High Fat Medium_12hr_rep2


title: Sk-hep1_Growth Medium_24hr_rep2

value: Growth Medium


title: KE-37 cell line, Control

value: KE-37 cell line, control sample


title: Senescence Replicate 1

city: Baltimore

first: Wenbing

last: Xie

state: MD

address: 1650 Orleans Street, CRB1, Room 530

department: Department of Oncology

country: USA

institute: The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, The Johns Hopkins University School of Medicine

description: Biological replicate 1 of 2. Early passage BJ cells were cultured about 28 population doublings to become senescent and then harvested.

hyb_protocol: Agilent human GE 4x 44K v2 microarrays (G4845A) were used, which contain 41,000 unique probes targeting 27,958 Entrez gene RNAs. 825 ng of each Cyanine-labeled samples was used for hybridization at 65ºC for 17 hours in a hybridization oven with rotation. After hybridization, microarrays are washed and dried according to Agilent microarray processing protocol in a walk-in ozone-controlled enclosure.

scan_protocol: Microarrays were scanned using an Agilent G2505C Scanner controlled by Agilent Scan Control 7.0 software.

data_processing: Data were extracted with Agilent Feature Extraction 9.5.3.1 software.

source_name: Senescent BJ fibroblast cells

label_protocol: Sample amplification and labeling procedures were carried out by using Quick RNA Amplification and Labeling Kit (Cat# 5190-0447, Agilent Technologies) with minor modifications. Briefly, 400 ng total RNA was reverse-transcribed into cDNA by MMLV-RT using oligo dT primers (Cat# RA300A-2, System Bioscience) that incorporate T7 promoter sequence. The cDNA is then used as a template for in vitro transcription in the presence of T7 RNA polymerase and Cyanine-labeled CTP (Perkin Elmer). The labeled cRNA is purified using RNeasy mini kit (Qiagen). RNA spike-in controls (Agilent Technologies) are added to RNA samples before amplification and labeling according to manufacturer’s protocol.

extract_protocol: Total RNA was isolated from cell pallets using TriZol reagent (Invitrogen) followed by RNeasy Mini Kit with DNase on-column digestion (Qiagen). RNA was quantified with NanoDrop ND-1000 followed by quality assessment with 2100 Bioanalyzer (Agilent Technologies) according to manufacturer’s protocol.

value: BJ

value: Foreskin

source_name: Early passage BJ fibroblast cells


title: BCK E2 ILC region replicate 1

city: Aurora

first: Britta

last: Jacobsen

state: CO

department: Pathology

institute: University of Colorado Anschutz Medical Campus

hyb_protocol: Hybridization, staining, and post-hybridization washes were completed according to the manufacturer's recommendations (Affymetrix).

scan_protocol: staining was captured using a gene array scanner 3000

data_processing: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.

source_name: BCK4 cells grown in nod scid mice

label_protocol: cDNA preparation using the Ovation Pico WTA System

growth_protocol: 5 million BCK4 cells were injected into the 4th mammary gland of NOD-SCID mice and grown for 5 months

extract_protocol: tumors frozen in OCT, sectioned on cryostat, 3000 cells were captured by LASER capture microdissection for each tumor region, RNA was isolated using the PicoPure RNA isolation Kit

treatment_protocol: cellulose or 17 beta estradiol

value: BCK4 breast cancer cells

tag: treatment

value: beta-estradiol (E2)

tag: tumor region

value: ILC region of mixed mucinous tumor


title: BCK E2 mucinous region replicate 2

value: mixed mucinous tumor region


title: BCK E2 ILC region replicate 2


title: Jurkat cell line, Control

value: Jurkat

value: Jurkat cell line, control sample


title: Early Transformation Replicate 3

description: Biological replicate 3 of 3. Early passage BJ cells were sequentially infected with genes encoding the human telomerase catalytic subunit (hTERT), the simian virus 40 small and large-T antigen (SV40) and the H-Ras oncoprotein (H-rasV12) to get the early transformed cells and then harvested.

source_name: Tranformed BJ fibroblast cells


title: Senescence Replicate 2

description: Biological replicate 2 of 2. Early passage BJ cells were cultured about 28 population doublings to become senescent and then harvested.


title: BCK cellulose replicate 2

value: cellulose

value: pure mucinous tumor


title: Mouse Xenograft Replicate 1

description: Biological replicate 1 of 3. Early transformed cells were injected in NOD-SCID immune deficient mice, and two monthes later, mouse xenografts were harvested.

source_name: Tranformed BJ fibroblast cells_xenograft


title: NGG1△/△ cells vs SC5314 cells (Grown in Spider Medium) Replicate 1

city: Beijing

first: Dedong

last: Li

state: Beijing

address: Fuxing Road 28

department: department of clinical pharmacology

country: China

institute: PLA General Hospital

description: Biological replicate 1 of 3. Control Candida albicans, grown in Spider medium for 1 h.

hyb_protocol: standard CapitalBio Methods

scan_protocol: Luxscan 10K (CapitalBio)

data_processing: After background correction and removal of bad spots, a space and intensity-dependent normalization based on a LOWESS program was employed. Two hybridizations were performed by using a reversal fluorescent strategy in one test and control and the other hybridization without performing reversal fluorescent.

organism: Candida albicans

source_name: Candida albicans

label_protocol: 10 µg of total RNA were primed with 2 µl of 100 µM T16N2 DNA primer at 70°C for 10 min, then reversed transcribed at 42°C for 1 h in the presence of 400 U SuperScript II RTase (Invitrogen), and 100 µM each dATP, dTTP, dGTP, with 25 µM dCTP, 25 µM Cy3-labe

growth_protocol: Strains were propagated in yeast-peptone-dextrose (YPD) medium (1% w/v yeast extract, 2% w/v peptone, 2% w/v dextrose).

extract_protocol: Total RNA was isolated by the hot phenol method.

treatment_protocol: Candida albicans cells were added to the culture medium (Spider medium) with the suspensions at 5×10^6 cells/ml. Following incubating statically at 37 °C at 1 h, the cells were collected and stored in liquid nitrogen.

tag: passage

value: Yeast-form cells incubated statically in Spider medium at 37 °C for 1h

tag: strain

value: Candida albicans ngg1delta/delta

value: Candida albicans SC5314

value: 1 h


title: Mouse Xenograft Replicate 2

description: Biological replicate 2 of 3. Early transformed cells were injected in NOD-SCID immune deficient mice, and two monthes later, mouse xenografts were harvested.


title: NGG1△/△ cells vs SC5314 cells (Grown in Spider Medium) Replicate 3

description: Biological replicate 3 of 3. Control Candida albicans, grown in Spider medium for 1 h.


title: NGG1△/△ cells vs SC5314 cells (Grown in Spider Medium) Replicate 2

description: Biological replicate 2 of 3. Control Candida albicans, grown in Spider medium for 1 h.


title: Early Transformation Replicate 2

description: Biological replicate 2 of 3. Early passage BJ cells were sequentially infected with genes encoding the human telomerase catalytic subunit (hTERT), the simian virus 40 small and large-T antigen (SV40) and the H-Ras oncoprotein (H-rasV12) to get the early transformed cells and then harvested.


title: H9 cell line, Control

value: CTL (Sezary syndrome)

value: H9 cell line, control sample


title: BCK cellulose replicate 1


title: Early Transformation Replicate 1

description: Biological replicate 1 of 3. Early passage BJ cells were sequentially infected with genes encoding the human telomerase catalytic subunit (hTERT), the simian virus 40 small and large-T antigen (SV40) and the H-Ras oncoprotein (H-rasV12) to get the early transformed cells and then harvested.


title: BCK E2 mucinous region replicate 1


title: Mouse Xenograft Replicate 3

description: Biological replicate 3 of 3. Early transformed cells were injected in NOD-SCID immune deficient mice, and two monthes later, mouse xenografts were harvested.


title: Mouse pancreatic islets purified by hand-picking at CT20, biological replicate 5

city: Lille

first: Lefebvre

last: Philippe

state: Nord

address: Blvd Pr. J.Leclerc

institute: Inserm

description: Mouse pancreatic islets isolated at CT20 by collagenase digestion, handpicking and  cultured for 24h (12h light/dark cycle, CT0 at 7am)

hyb_protocol: Hybridation was performed with the Affymetrix Hyb. Wash & Stain kit. Samples were denatured for 5 min. at 99°C, and hybridised for 16 hours at 45°C

scan_protocol: Affymetrix GeneChip Fluidics Station 450 and Scanner 3000 (Affymetrix)

data_processing: MTA_1. 0 arrays data files were analyzed using the Affymetrix expression console.

organism: Mus musculus

source_name: Pancreas

label_protocol: Samples were amplified using the Affymetrix WT Pico Amp Kit (module1). 3' adaptator and dscDNA synthesis were carried out using the Affymetrix WT Pico Amp Kit (module 2,3); cRNA production, purification were followed by fragmentation and labelling using the WTPico fragmentation kit.

growth_protocol: Mouse pancreatic islets from 10-week-old mice

extract_protocol: Animals were killed by cervical dislocationand islets were isolated isolated by collagenase P digestion (Boehringer Mannheim, Bromma, Sweden)The islets were cultured overnight in RPMI 1640 (SVA, Uppsala, Sweden) supplemented with 10% calf serum, 100 U ml−1 penicillin and 10 μg ml−1 streptomycin (all from Life Technologies, Täby, Sweden).

tag: time point

value: pancreatic islets

tag: gender

value: male

value: Pancreas


title: Mouse pancreatic islets purified by hand-picking at CT20, biological replicate 2


title: Mouse pancreatic islets purified by hand-picking at CT8, biological replicate 3

description: Mouse pancreatic islets isolated at CT8 by collagenase digestion, handpicking and  cultured for 24h (12h light/dark cycle, CT0 at 7am)


title: M. brunnea f.sp. Multigermtubi (M.b) treatment

type: SRA

city: Chongqing

first: Haitao

last: Xing

address: No.2 Tiansheng Road ,Beibei district

department: School of Life Sciences

institute: Southwest University

description:
processed data file: WT_vs_M_brunnea_f.sp_Multigermtubi_M.b_treatment.xls
processed data file: Results_of_DGE_analysis_of_PPR_family_genes_in_biotic_and_abiotic_treatment.xls

library_source: transcriptomic

data_processing:
Illumina Casava1.7 software used for basecalling.
1. Remove reads with adaptors; 2. Remove reads in which unknown bases are more than 10%; 3. Remove low quality reads (the percentage of the low quality bases of quality value ≤ 5 is more than 50% in a read).
Clean reads were mapped to reference sequences using SOAPaligner/soap2. Mismatches no more than 2 bases were allowed in the alignment.
Genome_build: 17273 P. trichocarpa full-length cDNAs were collected from the reference genome sequence of Populus (version 2.0, Phytozome).
Supplementary_files_format_and_content: WT_vs_*_treatment.xls: Excel format files include genes, RPKMs, and log2 ratios.
Supplementary_files_format_and_content: Results_of_DGE_analysis_of_PPR_family_genes_in_biotic_and_abiotic_treatment.xls: Excel format file includes PPR family genes and log2 ratios.

organism: Populus trichocarpa

source_name: leaves of the second and third internodes

growth_protocol: Populus trichocarpa were grown in a greenhouse at 25°C under a 14/10 h light/dark cycle.

extract_protocol:
The leaves applied for all stress treatments, pathogen infection, CK and DGE analysis were excised from the second and third internodes. Approximately 1 μg of total RNA per sample was incubated with oligo (dT) beads to capture the polyadenylated RNA fraction. Firststrand cDNA was synthesized using random hexamer-primer and reverse transcriptase (Invitrogen). The second-strand cDNA was synthesized using RNase H (Invitrogen) and DNA polymerase I (New England BioLabs).
The cDNA libraries were then prepared according to protocols of Illumina. To summarize, one individual single-end cDNA library was constructed for each sample, and then sequenced on the Illumina HiSeq 2000 platform for 35 cycles.

treatment_protocol: Plant material treatment: Hormone treatments: SA (5 mM in water) and MeJA [1mM in 0.1% (v/v) ethanol] were applied at the different concentrations as 5 ml droplets on each plant. The treated plants were immediately covered with a transparent lid. Fungal inoculation: The leaves were collected after 24 h. Leaves of three-month-old plants were inoculated with M. brunnea f.sp. multigermtubi. Mycelial plugs (6 mm) were placed on excised leaves (at least three leaves for each plant). These leaves were incubated in Petri dishes with humid filter paper in a humid chamber for 3 d. Low temperature stress: The healthy, well-hydrated plants were transferred to a growth chamber at 4°C under the same light and photoperiodic conditions for 1 h. After cold treatment, plants were allowed to recover at 20°C for 1 h. Wounding stress: For the wounding treatment, the young leaves of poplar plants were harvested after being punctured with sterile needles and placed at 20°C for 2 h. Salinity stress: The four-week-old seedlings were subjected to salt stress. Saline treatments had the NaCl concentrations of 100 mM added to full-strength Hoagland’s solution for 2 d. Wild type plant were grown in a greenhouse at 25°C under a 14/10 h light/dark cycle. Additionally, the leaves applied for all stress treatments, pathogen infection, CK and DGE analysis were excised from the second and third internodes.

value: Marssonina brunnea


title: MeJA treatment

description:
processed data file: WT_vs_MeJA_treatment.xls
processed data file: Results_of_DGE_analysis_of_PPR_family_genes_in_biotic_and_abiotic_treatment.xls

value: leaves of the second and third internodes

value: methyl jasmonate (MeJA)


title: SA treatment

description:
processed data file: WT_vs_SA_treatment.xls
processed data file: Results_of_DGE_analysis_of_PPR_family_genes_in_biotic_and_abiotic_treatment.xls

value: salicylic acid (SA)


title: Low temperature treatment

description:
processed data file: WT_vs_Low_temperature_treatment.xls
processed data file: Results_of_DGE_analysis_of_PPR_family_genes_in_biotic_and_abiotic_treatment.xls

value: cold


title: Mechanical wounding treatment

description:
processed data file: WT_vs_Mechanical_wounding_treatment.xls
processed data file: Results_of_DGE_analysis_of_PPR_family_genes_in_biotic_and_abiotic_treatment.xls

value: mechanical wounding


title: Mouse pancreatic islets purified by hand-picking at CT20, biological replicate 3


title: Mouse pancreatic islets purified by hand-picking at CT20, biological replicate 4


title: Wild type

description:
processed data files: WT_vs_*_treatment.xls
processed data file: Results_of_DGE_analysis_of_PPR_family_genes_in_biotic_and_abiotic_treatment.xls


title: KONT. M2

type: genomic

city: Warszawa

first: Krzysztof

last: Goryca

address: Banacha 2c

department: CeNT

country: Poland

institute: Uniwersytet Warszawski

hyb_protocol: As per manufacturer’s instructions (Illumina, San Diego CA)

scan_protocol: Arrays were scanned  on iScan

data_processing:
no data processing was applied
Thus the 'Matrix_signal_intensities.txt' file contains theta values from unprocessed file.

molecule: genomic DNA

source_name: control for pseudoexfoliation syndrome_male

label_protocol: DNA was assayed using Illumina SNP BeadChips as per manufacturer’s instructions (Illumina, San Diego CA).

extract_protocol: Genomic DNA was extracted from whole blood treated with EDTA using a QIAamp DNA Mini Kit

tag: clinical condition

value: control for pseudoexfoliation syndrome

tag: Sex

value: blood


title: LPS671

first: Helene

last: Hirbec

address: 141, rue de la Cardonille

department: Neuroscience

institute: IGF CNRS UMR5203

data_processing:
CRAC software (Philippe, Salson, Commes, & Rivals, 2013).
FeatureCounts tool was used to determine the number of reads mapping to each gene
Between 11.5 and 15.3 (13.6 1.3) million reads were then mapped on genes
Before normalization and statistical analysis, genes with less than 12 reads in at least 5 samples were filtered out
Genome_build: GRCm38 mm10
Supplementary_files_format_and_content: Text files include counts values for each Sampleand each gene. Raw-counts: all genes, no filering, no normalisation; Normalised_Rpkm; genes filtered for low expression and normalised; Normalised_Rpkm_coding: genes filtered for low expression and normalised, limited to coding genes

source_name: Facs isolated microglia cells, intraperitoneal LPS

extract_protocol:
Mice were perfused intracardially with PBS. The cortex were dissected and homogenized in a glass-teflon dounce homogenizer. Leukocytes were prepurified using percoll gradient. Cells expressing jigh level of GFP were isolated by FACS.
5 ng of total RNA were amplified using the SMARTer ultra low RNA kit (Clontech) and 6 ng of amplified RNA wereused for  librairies construction  using the Ovation ultra low kit from Nugen

value: microglia cells

value: female

tag: genotype

value: CX3CR1+/egfp mice

value: 2-3 months


title: LPS630

tag: tissue


title: LPS700


title: PEX M2

source_name: pseudoexfoliation syndrome_male

value: pseudoexfoliation syndrome


title: KONT. M1


title: LPS697


title: KONT. M3


title: Salinity treatment

description:
processed data file: WT_vs_Salinity_treatment.xls
processed data file: Results_of_DGE_analysis_of_PPR_family_genes_in_biotic_and_abiotic_treatment.xls

value: salinity


title: exomiRNAs

city: Wuhan

first: Wu

last: Qi

state: Hubei

address: 99 Zhang Zhidong Road

department: Department of Breast and Thyroid Surgery

institute: Renmin Hospital of Wuhan University

data_processing:
A proprietary pipeline script, ACGT101-miR v4.2 (LC Sciences), was used for sequencing data analysis
Supplementary_files_format_and_content: counts

source_name: exosomes from MDA-MB-231 cultivated with mature 3T3-L1

growth_protocol: The breast cancer cell line MDA-MB-231 and mouse preadipocytes line 3T3-L1 were obtained from the American Type Culture Collection (Manassas, VA). MDA-MB-231 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% free-exosome fetal bovine serum (FBS, Shin Chin Industrial, SCI) and 1 % penicillin–streptomycin (Hyclone, Logan, UT, USA) in a humidified 37 ℃ incubator supplemented with 5% CO2. Preadipocytes 3T3-L1, obtained from ATCC (Shanghai), were cultured in DMEM supplemented with 10% fetal calf serum (FCS, Gibico) and 1 % penicillin–streptomycin (Hyclone, Logan, UT, USA) in a humidified 37 ℃ incubator supplemented with 5% CO2 and were differentiated as previously report.

extract_protocol:
To isolate exosomes, cells were cultured with exosome-depleted serum (Shin Chin Industrial, SCI). We collected the conditioned medium to isolate exosomes according to the instructions83. The medium from cell culture was centrifuged at 500 g for five minutes followed by 2,000 g for thirty minutes at 4 °C to remove cellular debris and large apoptotic bodies. Once centrifuged, the media was added to an equal volume of a 2× polyethylene glycol (PEG, MW 6000, Sigma, 81260) solution (final concentration 8%) at 4 °C. Before incubated at 4 °C overnight, samples were mixed thoroughly by inversion. Samples were further centrifuged in a tabletop centrifuge at maximum speed (15,000 rpm) for 1 hour at 4 °C. After the practice, the tubes were allowed to tap occasionally and drain for five minutes to remove excess PEG. The resulting pellets were further purified for a second time using a 5 % concentration of PEG and were then stored at – 80 ℃ after suspended in 50–500 μl of particle-free PBS (pH 7.4). The average yield of approximately 300 g exosome protein which was produced from 5 ml of supernatant. One was subjected to RNA extraction using Trizol reagent (Life Technologies) followed by miRNA assessment by microarrays and RT-PCR described as follow. The characteristics of exosomes were analyzed by electron microscopy to verify the presence, nanoparticle characterization system to measure the size and determine concentration of exosomes, and western bolt to detect the proteins (HSP70, TSG101, CD63 and CD81) of exosomes.
Isolation of miRNA from exosomes was conducted with the miRNeasy Kit (Qiagen, USA), following the manufacturer’s instructions. miRNA levels were performed using TruSeq Small RNA Sample Prep Kits (Illumina, San Diego, USA), according to the manufacturer’s instructions. Each library was indexed in order to multiplex  samples per sequencing run on the Illumina MiSeq platform using MiSeq 50 cycle Reagent Kits v2. A minimum of 17 million 50bp sequencing reads were collected from each sample and data were analyzed using Genesifter software (formerly Geospiza) (PerkinElmer, Santa Clara CA). Raw data for each sample were aligned to the most recent mirBASE database (mirBase.org [17]) with remaining reads aligned to the most recent human genome (hg18 build [18]) build in order to identify previously unknown regions that may encode for unique miRNAs. Pairwise comparison of the alignment results was done using Genesifter for identification of miRNAs that are differentially expressed at a significant level, i.e. up- or down-regulated.

treatment_protocol: Breast cancer cells and mature adipocytes were co-cultured using Transwell culture plates (0.4-m pore size; Millipore). A total of 3 x 105 (MCF-7, MDA-MB-231) cells were seeded in the top chamber of the Transwell system in the culture medium of adipocytes and cultivated with or without mature adipocytes in the bottom chamber for the indicated times. Mature adipocytes were cultivated 4 hours with serum-free medium containing 1% of bovine serum albumin (Sigma). The cancer-conditioned mediums were collected from cells cultivated alone in similar conditions for 3 days served as controls, while cancer-associated adipocytes conditioned medium (CA-CM) was collected from adipocytes cultivated with tumor cells for 3 days.

value: MDA-MB-231 cultivated with mature 3T3-L1 for 3 days


title: KONT. K6

source_name: control for pseudoexfoliation syndrome_female


title: KONT. K3


title: KONT. K4


title: PBS703

source_name: Facs isolated microglia cells, saline solution injection


title: KONT. K7


title: PBS673


title: KONT. K5


title: PBS672


title: KONT. K2


title: Mouse pancreatic islets purified by hand-picking at CT8, biological replicate 2

value: pancreatic islets

value: male


title: Mouse pancreatic islets purified by hand-picking at CT8, biological replicate 5

value: Pancreas


title: Mouse pancreatic islets purified by hand-picking at CT8, biological replicate 1


title: Mouse pancreatic islets purified by hand-picking at CT8, biological replicate 4


title: PEX M3


title: Mouse pancreatic islets purified by hand-picking at CT20, biological replicate 1


title: PEX K6

source_name: pseudoexfoliation syndrome_female


title: PEX M1


title: LPS622


title: PEX K4


title: PBS629


title: KONT. K1


title: PEX K5


title: PEX K3


title: PBS621


title: PEX K1


title: Nfkb2-KO Treg, rep.2

city: Lyon

first: Yenkel

last: Grinberg-Bleyer

address: 28 rue Laennec

department: Immunity, viruses and inflammation

institute: Cancer Research Center of Lyon

description: YS 041

data_processing:
raw FASTQ files were aligned on the mm10 genome using STAR aligner with default parameters
Aligned fragments were then counted and annotated using Rsamtools v3.2 and the TxDb.Mmusculus.UCSC.mm10.knownGene’ version 3.1.2 transcript database
Normalized FPKM (fragments per kilobase per million mapped reads) were obtained using the robust FPKM estimate function of DeSeq2 v1.10.1 after removing the batch effect using the ComBat function of the sva package v3.18.0
Differentially expressed genes were obtained using the DESeqResults function of the same package
All p-values were adjusted for mutliple comparison using the Benjamini & Hochberg FDR algorithm
Genome_build: mm10

source_name: Sorted cells from pooled spleen and lymph nodes

growth_protocol: Cells were stimulated in complete RPMI medium, in 12-well tissue culture plates coated with 5 ug/ml anti-mCD3, in the presence of 1ug/mL soluble anti-mCD28, 2ug/ml agonist anti-OX40 Ab, and 10 ng/mL murine IL-2, at 37°C for 16 hours.

extract_protocol:
RNA was prepared using the Qiagen Rneasy Mini Kit reagents and protocol.
RNA libraries were prepared for sequencing using the TruSeq Sample Prepr Kit v2

value: Foxp3-CRE-YFP+ Treg cells

tag: strain/genotype

value: C57Bl/6 Foxp3-CRE-YFP*nfkb2 F/F


title: PEX K2


title: WT Treg, rep.2

description: YS 040

value: C57Bl/6 Foxp3-CRE-YFP


title: relbKO Tregs, rep.2

description: YS 042

value: C57Bl/6 Foxp3-CRE-YFP*RelB F/F


title: PEX K7


title: Relb-Nfkb2-DKO Treg, rep.1

description: YS 018

value: C57Bl/6 Foxp3-CRE-YFP*RelB F/F*nfkb2 F/F


title: Relb-Nfkb2-DKO Treg, rep.2

description: YS 043


title: Nfkb2-KO Treg, rep.1

description: YS 017


title: Vit C-treated 24 h_ Rep 2

city: Bangalore

first: Genotypic

last: technology

state: Karnataka

address: 259, Apoorva 4th cross,80 feet Road,RMV 2ND STAGE

country: India

institute: Genotypic Technology

description: Biological replicate 2 of 3, aerobically grown OD595~0.1-0.2, 10 mM AA treated for 24 h

hyb_protocol: 600ng of labeled cRNA sample were fragmented at 60 º C and hybridized on to a Custom M.tb GXP_8X15k Array designed by Genotypic Technology Private Limited. (AMADID: 026323). Fragmentation of labeled cRNA and hybridization were done using the Gene Expression Hybridization kit of (Agilent Technologies, In situ Hybridization kit, Part Number 5190-0404). Hybridization was carried out in Agilent’s Surehyb Chambers at 65º C for 16 hours.

scan_protocol: Agilent Microarray Scanner (Agilent Technologies, Part Number G2600D).

data_processing: Images were quantified using Feature Extraction Software ( Agilent). Feature extracted raw data was analyzed using GeneSpring GX software from Agilent. Normalization of the data was done in GeneSpring GX using the percentile shift normalization method .

organism: Mycobacterium tuberculosis H37Rv

source_name: T 24 II

label_protocol: The samples for Gene expression were labeled using Agilent Quick-Amp labeling Kit (p/n5190-0442). 500ng each of total RNA were reverse transcribed at 40°C using oligodT primer with T7 polymerase promoter converted to double stranded cDNA. Synthesized double stranded cDNA were used as template for cRNA generation. cRNA was generated by in vitro transcription and the dye Cy3 CTP(Agilent) was incorporated during this step. The cDNA synthesis and in vitro transcription steps were carried out at 40°C. Labeled cRNA was cleaned up using Qiagen RNesay columns (Qiagen, Cat No: 74106) and quality assessed for yields and specific activity using the Nanodrop ND-1000.

growth_protocol: M. tuberculosis culture was grown aerobically in Dubos medium supplemented with 0.5% BSA, 0.75% Glucose and 0.085% NaCl plus 0.1% Tween-80  (DTA medium) untill logarithmic phase (OD595 ~0.4-0.5).

extract_protocol: Total M. tuberculosis RNA was extracted using Trizol based extraction protocol.

treatment_protocol: Logarithmic phase culture of M. tuberculosis grown to an OD595~ 0.1-0.2 in DTA medium followed by addition of 10 mM of vitamin C for 0.25 to 24 hours under shaking condition at 37°C.

tag: agent

value: Vit C

tag: protocol

value: Aerobically grown


title: Vit C-treated 24 h_ Rep 1

description: Biological replicate 1 of 3, aerobically grown OD595~0.1-0.2, 10 mM AA treated for 24 h

source_name: T 24 I


title: Vit C-treated 24 h_ Rep 3

description: Biological replicate 3 of 3, aerobically grown OD595~0.1-0.2, 10 mM AA treated for 24 h

source_name: T 24 IV


title: Vit C-treated 4 h_ Rep 3

description: Biological replicate 3 of 3, aerobically grown OD595~0.1-0.2, 10 mM AA treated for 4 h

source_name: T 4 III

value: 4 h


title: Vit C-treated 8 h_ Rep 3

description: Biological replicate 3 of 3, aerobically grown OD595~0.1-0.2, 10 mM AA treated for 8 h

source_name: T 8 IV

value: 8 h


title: Vit C-treated 8 h_ Rep 2

description: Biological replicate 2 of 3, aerobically grown OD595~0.1-0.2, 10 mM AA treated for 8 h

source_name: T 8 III


title: Vit C-treated 8 h_ Rep 1

description: Biological replicate 1 of 3, aerobically grown OD595~0.1-0.2, 10 mM AA treated for 8 h

source_name: T 8 I


title: RelB-KO Treg, rep.1

description: YS 016

tag: cell type


title: WT Treg, rep.1

description: YS 015


title: PHH vehicle control (sub 421)

city: Madison

first: Srikumar

last: Sengupta

state: WI

address: 330 N Orchard St

department: Regenerative Biology

institute: Morgridge Institute for Research

data_processing:
Basecalls performed using CASAVA version 1.8.2
Reads filtered to eliminate adapter sequence and low-quality reads
Gene expression estimates obtained using RSEM v 1.2.3, with Bowtie 0.12.9 for alignment. rsem-calc-expression used default parameters, plus: "--seed-length 28 "
Genome_build: hg19, mm10
Supplementary_files_format_and_content: excel spreadsheet containing RSEM Expected Counts and Transcripts per Million in separate worksheets

source_name: Life Technologies, batch used: Hu4260

extract_protocol:
Total RNA were isolated using RNAeasy Plus Kit (Qiagen) following manufacturers protocol and qualified with the Life Technologies Qubit fluorometer and Agilent Bioanalyzer.
cDNA libraries were prepped using the LM-Seq protocol described in  Hou et al 2015 (PMID 25831155).
Indexed samples were multiplexed on an Illumina HiSeq 2500

value: hepatocyte

tag: growth protocol

value: Hepatocyte media (Promocell) on Matrigel coated plates

tag: treatment protocol

value: DMSO, 72 hours


title: PHH PD (sub 427)


title: Vit C-treated 4 h_ Rep 2

description: Biological replicate 2 of 3, aerobically grown OD595~0.1-0.2, 10 mM AA treated for 4 h

source_name: T 4 II


title: Vit C-treated 4 h_ Rep 1

description: Biological replicate 1 of 3, aerobically grown OD595~0.1-0.2, 10 mM AA treated for 4 h

source_name: T 4 I


title: Vit C-treated 0.25 h_ Rep 2

description: Biological replicate 2 of 3, aerobically grown OD595~0.1-0.2, 10 mM AA treated for 0.25 h

source_name: T 15 II


title: Vit C-treated 1 h_ Rep 2

description: Biological replicate 2 of 3, aerobically grown OD595~0.1-0.2, 10 mM AA treated for 1 h

source_name: T 1 II

value: 1 h


title: Vit C-treated 1 h_ Rep 1

description: Biological replicate 1 of 3, aerobically grown OD595~0.1-0.2, 10 mM AA treated for 1 h

source_name: T 1 I


title: PHH UO126 (sub 421)

value: Uo126, 72 hours


title: Vit C-treated 2 h_ Rep 3

description: Biological replicate 3 of 3, aerobically grown OD595~0.1-0.2, 10 mM AA treated for 2 h

source_name: T 2 III

value: 2 h


title: Vit C-treated 0.25 h_ Rep 1

description: Biological replicate 1 of 3, aerobically grown OD595~0.1-0.2, 10 mM AA treated for 0.25 h

source_name: T 15 I


title: Vit C-treated 2 h_ Rep 2

description: Biological replicate 2 of 3, aerobically grown OD595~0.1-0.2, 10 mM AA treated for 2 h

source_name: T 2 II


title: Vit C-treated 1 h_ Rep 3

description: Biological replicate 3 of 3, aerobically grown OD595~0.1-0.2, 10 mM AA treated for 1 h

source_name: T 1 III


title: Vit C-treated 0.5 h_ Rep 2

description: Biological replicate 2 of 3, aerobically grown OD595~0.1-0.2, 10 mM AA treated for 0.5 h

source_name: T 30 II


title: Vit C-treated 0.5 h_ Rep 1

description: Biological replicate 1 of 3, aerobically grown OD595~0.1-0.2, 10 mM AA treated for 0.5 h

source_name: T 30 I


title: Vit C-treated 2 h_ Rep 1

description: Biological replicate 1 of 3, aerobically grown OD595~0.1-0.2, 10 mM AA treated for 2 h

source_name: T 2 I


title: Vit C-treated 0.25 h_ Rep 3

description: Biological replicate 3 of 3, aerobically grown OD595~0.1-0.2, 10 mM AA treated for 0.25 h

source_name: T 15 III


title: Vit C-treated 0.5 h_ Rep 3

description: Biological replicate 3 of 3, aerobically grown OD595~0.1-0.2, 10 mM AA treated for 0.5 h

source_name: T 30 III


title: Control (Untreated) Rep 1

description: Biological replicate 1 of 3, aerobically grown OD595~0.1-0.2, untreated control

source_name: T con I

value: Control (Untreated)


title: Control (Untreated) Rep 2

description: Biological replicate 2 of 3, aerobically grown OD595~0.1-0.2, untreated control

source_name: T con II


title: Control (Untreated) Rep 3

description: Biological replicate 3 of 3, aerobically grown OD595~0.1-0.2, untreated control

source_name: T con III


title: uncultured PHH mixture (sub 365)

source_name: Life Technologies, batches used: Hu4260, Hu8135, Hu4279 and Hu8137

extract_protocol:
Total RNA were isolated using RNAeasy Plus Kit (Qiagen) following manufacturers protocol and qualified with the Life Technologies Qubit fluorometer and Agilent Bioanalyzer.
cDNA libraries were prepared using Illumina’s TruSeq RNA Sample Preparation Kit v2 (RS-122-2001 and RS-122-2002).
Indexed samples were multiplexed on an Illumina HiSeq 2500

value: Uncultured

value: none, 0 hours


title: HepG2 vehicle econtrol (sub 421)

source_name: ATCC

tag: cell line

value: DMEM F12 + 10% FBS

value: DMSO, 0 hours


title: HepG2 uo126 (sub 421)


title: PHH mixture cultured 24 hours (sub 353)

value: none, 24 hours


title: PHH 2 (sub 353)

source_name: Life Technologies, batch used: Hu4260


title: SC_microRNA_Cell_BaF3_R203

type: other

city: Utrecht

last: VanInsberghe

state: Utrecht

address: Uppsalalaan 8

country: Netherlands

institute: Hubrecht Institute

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R203

hyb_protocol: not applicable

scan_protocol: not applicable

data_processing: Raw CT values were normalized to the cut-off CT derived from the distribution of single-cDNA-molecule amplification events

label: FAM

molecule: other

source_name: BaF3 single cell

label_protocol: not applicable

growth_protocol: IL-3 independent BaF3 cells were cultured in RPMI-1640 (Gibco) supplemented with 10% FBS and 1× GlutaMAX.

extract_protocol: Single cells were lysed in situ

tag: type

value: Cell


title: SC_microRNA_Cell_BaF3_R204

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R204


title: SC_microRNA_Cell_BaF3_R206

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R206


title: SC_microRNA_Cell_BaF3_R208

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R208


title: SC_microRNA_Cell_BaF3_R207

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R207


title: SC_microRNA_Cell_BaF3_R205

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R205


title: 3rd ∆asp5_2

city: Stanford

first: Adit

last: Naor

state: CA

address: 300 Pasteur Drive Stanford, D305 Fairchild Building

institute: Stanford

description: 3rd experiment

data_processing:
Illumina Nextseq 500 model instrument was used for sequencing, Sequenced reads were trimmed for adaptor sequence.
Raw reads were uploaded onto the CLC Genomics Workbench 8.0 (Qiagen) platform for independent alignments against the human genomes (Ensembl.org/ hg19) and Toxoplasma Type I GT1 strain (ToxoDB-24, GT1 genome)
Supplementary_files_format_and_content: tab-delimited text file includes number of reads per gene for each Sample

source_name: 3rd ∆asp5

growth_protocol: HFFs were serum-starved for 24 hours before infection by growth in DMEM containing 0.5% serum. They were then infected with the indicated line of tachyzoites at an MOI of 5, for  6hours.  RNA samples were submitted to the Stanford University Functional Genomic Facility (SFGF) for purity analysis using the Agilent 2100 Bioanalyzer. Multiplex sequencing libraries were generated with RNA Sample Prep Kit (Illumina) according to manufacturer’s instructions and pooled for a single high-throughput sequencing run using the Illumina NextSeq platform (Illumina Nextseq 500 model instrument).

extract_protocol:
1 ml TRIzol reagent (Invitrogen) was added to each T25 and the cells were scraped. Lysates were collected into RNAse/DNAse-free Eppendorf tubes and frozen at −20 °C. Total RNA was extracted following the manufacturer’s instructions, with some modifications. Briefly: frozen samples were thawed on ice and 0.2 ml chloroform was added to TRIzol suspensions, which were then mixed by inverting 10 times, and incubated for 5 minute. Tubes were then spun at 12,000 rpm for 15 min at 4 °C. RNA in the aqueous phase was transferred into a fresh tube and 0.5 ml absolute isopropyl alcohol was added. Each tube was inverted three times and incubated at 4°C for 10 min. They were then spun at 12,000 rpm for 20 min at 4 °C. After decanting the supernatants, RNA pellets were washed with 1 ml 75% ethanol. Tubes were mixed by inverting 10 times and then spun at 12,000 rpm for 20 min at 4°C. Supernatants were removed and the RNA pellets were air-dried in open tubes for approximately 10 min. The RNA pellets were resuspended in 30 μl RNase-free DEPC-water.
NA samples were submitted to the Stanford University Functional Genomic Facility (SFGF) for purity analysis using the Agilent 2100 Bioanalyzer. Multiplex sequencing libraries were generated with RNA Sample Prep Kit (Illumina) according to manufacturer’s instructions and pooled for a single high-throughput sequencing run using the Illumina NextSeq platform (Illumina Nextseq 500 model instrument).

value: HFF

tag: infection

value: ∆asp5 parasites

tag: time

value: 6 hours post infection


title: 3rd ∆myr1::MYR1_3

description: 3rd experiment

source_name: 3rd ∆myr1::MYR1

value: ∆myr1::MYR1 parasites


title: 3rd ∆asp5::ASP5_1

description: 3rd experiment

source_name: 3rd ∆asp5::ASP5

value: ∆asp5::ASP5 parasites


title: 3rd ∆myr1_3

description: 3rd experiment

source_name: 3rd ∆myr1

value: ∆myr1 parasites


title: 3rd ∆asp5::ASP5_2

description: 3rd experiment


title: 3rd ∆asp5::ASP5_3

description: 3rd experiment


title: SC_microRNA_Cell_BaF3_R198

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R198


title: 3rd ∆asp5_1

description: 3rd experiment


title: SC_microRNA_Cell_BaF3_R193

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R193


title: SC_microRNA_Cell_BaF3_R191

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R191


title: 3rd ∆myr1::MYR1_2

description: 3rd experiment


title: SC_microRNA_Cell_BaF3_R201

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R201


title: SC_microRNA_Cell_BaF3_R194

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R194


title: SC_microRNA_Cell_BaF3_R197

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R197


title: SC_microRNA_Cell_BaF3_R196

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R196


title: SC_microRNA_Cell_BaF3_R199

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R199


title: SC_microRNA_Cell_BaF3_R195

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R195


title: SC_microRNA_Cell_BaF3_R200

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R200


title: 3rd ∆asp5_3

description: 3rd experiment


title: 3rd ∆myr1::MYR1_1

description: 3rd experiment


title: 3rd ∆myr1_2

description: 3rd experiment


title: SC_microRNA_Cell_BaF3_R190

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R190


title: SC_microRNA_Cell_BaF3_R189

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R189


title: 2nd ∆asp5::ASP5_2

description: 2nd experiment

source_name: 2nd ∆asp5::ASP5


title: SC_microRNA_Cell_BaF3_R183

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R183


title: SC_microRNA_Cell_BaF3_R192

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R192


title: 3rd Mock_2

description: 3rd experiment

source_name: 3rd Mock

value: Mock infected


title: 3rd RH_1

description: 3rd experiment

source_name: 3rd RH

value: RH parasites


title: 3rd ∆myr1_1

description: 3rd experiment


title: 2nd ∆asp5::ASP5_1

description: 2nd experiment


title: SC_microRNA_Cell_BaF3_R181

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R181


title: 2nd ∆asp5_2

description: 2nd experiment

source_name: 2nd ∆asp5


title: 2nd ∆myr1::MYR1_2

description: 2nd experiment

source_name: 2nd ∆myr1::MYR1


title: SC_microRNA_Cell_BaF3_R186

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R186


title: SC_microRNA_Cell_BaF3_R175

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R175


title: 3rd RH_3

description: 3rd experiment


title: 3rd RH_2

description: 3rd experiment


title: SC_microRNA_Cell_BaF3_R180

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R180


title: SC_microRNA_Cell_BaF3_R202

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R202


title: SC_microRNA_Cell_BaF3_R187

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R187


title: 2nd ∆asp5_1

description: 2nd experiment


title: SC_microRNA_Cell_BaF3_R179

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R179


title: 3rd Mock_1

description: 3rd experiment


title: 3rd Mock_3

description: 3rd experiment


title: SC_microRNA_Cell_BaF3_R176

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R176


title: 2nd ∆myr1::MYR1_1

description: 2nd experiment


title: SC_microRNA_Cell_BaF3_R171

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R171


title: SC_microRNA_Cell_BaF3_R173

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R173


title: SC_microRNA_Cell_BaF3_R172

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R172


title: SC_microRNA_Cell_BaF3_R174

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R174


title: 2nd ∆myr1_2

description: 2nd experiment

source_name: 2nd ∆myr1


title: SC_microRNA_Cell_BaF3_R168

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R168


title: 2nd RH_2

description: 2nd experiment

source_name: 2nd RH


title: SC_microRNA_Cell_BaF3_R170

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R170


title: SC_microRNA_Cell_BaF3_R169

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R169


title: 2nd ∆myr1_1

description: 2nd experiment


title: 2nd Mock_2

description: 2nd experiment

source_name: 2nd Mock


title: 2nd RH_1

description: 2nd experiment


title: SC_microRNA_Cell_BaF3_R167

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R167


title: SC_microRNA_Cell_BaF3_R166

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R166


title: DepPhosphate72h_rep2

city: Trondheim

first: Per

last: Winge

address: Høgskoleringen 5e

department: Department of Biology

country: Norway

institute: Norwegian University of Science and Technology

description: Sample name: DP 72h(II)

hyb_protocol: 1650 ng cRNA from each sample was fragmented and hybridized on 4x44K P. tricornutum whole-genome 60-mer oligonucleotide microarrays (Agilent Technologies) in an Agilent G2545A Hybridization rotary oven (10 rpm, 65°C, 17.5 h). Hybridization was performed with the Gene Expression Hybridization Kit (Agilent p/n 5188-5242). The slides were washed with buffer 1 & 2 from Gene Expression Wash Buffer kit (Agilent p/n 5188-5327)

scan_protocol: The microarray sides were scanned twice at 5 µm resolution on a laser scanner (G2505 B from Agilent Technologies), using the “dynamic range expander” option in the scanner software. The resulting images were processed using Agilent Feature Extraction software v9.5.

data_processing: The median signal intensities (gMedianSignal) were normalized using the quantile method, and no background subtraction was performed. Spots identified as feature outliers were excluded from analysis, and weak or non-detected spots were given reduced weight (0.5). Genes with statistically significant differential expression were identified using the Limma package (version 3.2.3) and R (version 2.10.1).

organism: Phaeodactylum tricornutum

source_name: Phaeodactylum, Depleted phosphate, 72 h

label_protocol: Total RNA (83 - 200 ng) was reverse transcribed, amplified and labelled with Cy3-CTP using the Low Input Quick Amp Labelling Kit, One-Color (Agilent p/n 5190-2305)

growth_protocol: Cultures were grown in f/2 medium made from 0.2 µm filtered sea water, autoclaved and enriched with vitamins and inorganic nutrients. Axenic cultures were kept in exponential growth at 15ºC under continuous white fluorescent light (60 µmol photons m-2 s-1) inside the shaking incubator for three weeks.

extract_protocol: Depending on cell density, 60-100 ml of cultures were collected on 0.65 µm Durapore membrane filters, washed off the filter using 1 ml of f/2 medium (P-deplete cells were washed with f/2 without phosphate supplement), and centrifuged at 13000 rpm for 1 min at 4°C. The supernatant was removed and pellets were flash frozen in liquid nitrogen and stored at -80°C. Precooled (-80°C) 5 mm stainless steel beads (QIAGEN) were added to the tubes with frozen cell pellets, and the samples were mechanically disrupted and homogenized using the TissueLyser system (QIAGEN). Disruption was carried out for 2 x 2 min at 25 Hz. The samples were placed in a precooled (-80°C) adapter set for the first shaking step. Before the second shaking step, the samples were transferred to a room temperate adapter set and 0.5 ml lysis buffer (SpectrumTM Plant Total RNA kit, Sigma-Aldrich) was added to each tube. Total RNA was isolated from the homogenized lysate using the SpectrumTM Plant Total RNA kit (Sigma-Aldrich). On-column digestion of DNA with DNase I (QIAGEN) was included for all RNA preparations. The concentration of the RNA was determined by measuring the absorbance at 260 nm with the NanoDrop ND-1000 Spectrophotometer (NanoDrop Technologies), and the purity of the RNA was estimated by the OD260/OD280 nm absorption ratio (all ratios were > 2.0). The integrity of the RNA was verified by denaturing agarose gel electrophoresis and ethidium bromide staining following the protocol described in the RNeasy Mini handbook (QIAGEN).

treatment_protocol: To start the experiment, three replicates and parallels of the start culture were transferred to media supplemented with complete f/2 nutrients (replete) or f/2 without phosphate (deplete). Cells were incubated in batch cultures with a start cell density of 5×104 ml-1 in 75 cm2 sterile culture flasks and volume of 220 ml. sampling for Cell counting and variable in vivo Chl a fluorescence (Fv/Fm) was done daily . For the other experiments, samples were harvested 48 h and 72 h after the beginning of the treatment.

tag: clone

tag: treatment

value: Depleted phosphate


title: 1st ∆myr1::MYR1_2

description: 1st experiment

source_name: 1st ∆myr1::MYR1


title: 2nd Mock_1

description: 2nd experiment


title: Control72h_rep3

description: Sample name: CTRL 72h(III)

source_name: Phaeodactylum, Control, 72 h

value: Control


title: SC_microRNA_Cell_BaF3_R164

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R164


title: DepPhosphate72h_rep3

description: Sample name: DP 72h(III)


title: Control72h_rep2

description: Sample name: CTRL 72h(II)


title: Control48h_rep2

description: Sample name: CTRL 48h(II)

source_name: Phaeodactylum, Control, 48 h


title: DepPhosphate72h_rep1

description: Sample name: DP 72h(I)


title: Control72h_rep1

description: Sample name: CTRL 72h(I)


title: SC_microRNA_Cell_BaF3_R165

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R165

source_name: BaF3 single cell


title: DepPhosphate48h_rep1

description: Sample name: DP 48h(I)

source_name: Phaeodactylum, Depleted phosphate, 48 h


title: ColonAXDSSCT465

city: VILLEJUIF

first: christophe

last: desterke

address: 14-16 avenue Paul Vaillant Couturier

department: UFR medecine institute Andre Lwoff

institute: university Paris Sud11

description: ColonAXDSSCT

hyb_protocol: 1.9 μg of cDNAs were hybridized for 16 hours at 45oC on GeneChip® Mouse Gene 1.0 ST arrays (Affymetrix) interrogating 28.853 genes represented by approximately 27 probes spread across the full length of the gene. The chips were washed and stained in the GeneChip® Fluidics Station 450 (Affymetrix).

scan_protocol: The chips were scanned with the GeneChip® Scanner 3000 7G (Affymetrix) at a resolution of 0.7 µm.

data_processing: CEL files were further processed with Affymetrix Expression Console software version 1.1 to calculate probe set signal intensities using Robust Multi-array Average (RMA) algorithms with default settings.

label: terminal deoxynucleotidyl transferase (TdT)

source_name: Colon tissues

label_protocol: Biotinylated single strand cDNA targets were prepared, starting from 200 ng of total RNA, using the Ambion WT Expression Kit (Cat # 4411974) and the Affymetrix GeneChip® WT Terminal Labeling Kit (Cat # 900671) according to Affymetrix recommendations.

growth_protocol: Colon tissues were collected at day 12 of treatment, flash frozen in liquid nitrogen and stored at -80°C until total RNA extraction.

extract_protocol: Total RNA extracts were prepared using the Ambion mirVana miRNA Isolation Kit (#AM1560) according to Ambion recommendations.

treatment_protocol: One control group of untreated mice were given free water, whereas a second group of mice received 3% DSS ad libitum for 5 days, then free water until sacrifice, and finally a third group composed of axenic mice received, 3 weeks after fecal microbiota transplantation, 3% DSS ad libitum for 5 days, then free water until sacrifice.

value: Colon

tag: phenotype

value: axenic

tag: treatment group

value: DSS before the transfer of the intestinal microbe

tag: strain


title: DepPhosphate48h_rep3

description: Sample name: DP 48h(III)


title: DepPhosphate48h_rep2

description: Sample name: DP 48h(II)


title: Control48h_rep1

description: Sample name: CTRL 48h(I)


title: ColonAXDSSCT517

description: ColonAXDSSCT


title: Control48h_rep3

description: Sample name: CTRL 48h(III)


title: ColonAXDSSCT229

description: ColonAXDSSCT


title: SC_microRNA_Cell_BaF3_R163

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R163


title: ColonAXDSSCT093

description: ColonAXDSSCT


title: 1st ∆myr1_2

description: 1st experiment

source_name: 1st ∆myr1


title: 1st ∆myr1::MYR1_1

description: 1st experiment


title: ColonAXDSSCT677

description: ColonAXDSSCT


title: SC_microRNA_Cell_BaF3_R162

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R162


title: 1st ∆myr1_1

description: 1st experiment


title: ColonAXDSSTG713

description: ColonAXDSSTG

value: DSS after the TG intestinal microbial transfer


title: ColonAXDSSTG632

description: ColonAXDSSTG


title: ColonAXDSSTG922

description: ColonAXDSSTG


title: SC_microRNA_Cell_BaF3_R159

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R159


title: ColonAXDSSTG749

description: ColonAXDSSTG


title: 1st RH_2

description: 1st experiment

source_name: 1st RH


title: 1st Mock_2

description: 1st experiment

source_name: 1st Mock


title: ColonAXDSSTG446

description: ColonAXDSSTG


title: SC_microRNA_Cell_BaF3_R160

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R160


title: 1st Mock_1

description: 1st experiment


title: SC_microRNA_Cell_BaF3_R152

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R152


title: SC_microRNA_Cell_BaF3_R153

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R153


title: 1st RH_1

description: 1st experiment


title: ColonAXDSSWT597

description: ColonAXDSSWT

value: DSS after the WT intestinal microbial transfer


title: ColonAXDSSWT098

description: ColonAXDSSWT


title: ColonAXDSSWT793

description: ColonAXDSSWT


title: SC_microRNA_Cell_BaF3_R149

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R149


title: ColonAXDSSWT880

description: ColonAXDSSWT


title: ColonAXDSSWT639

description: ColonAXDSSWT


title: ColonDSSTG44

description: ColonDSSTG


title: SC_microRNA_Cell_BaF3_R151

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R151


title: SC_microRNA_Cell_BaF3_R150

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R150


title: SC_microRNA_Cell_BaF3_R145

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R145


title: ColonDSSTG47

description: ColonDSSTG


title: ColonDSSWT52

description: ColonDSSWT


title: ColonDSSTG46

description: ColonDSSTG


title: ColonDSSTG49

description: ColonDSSTG


title: ColonDSSWT53

description: ColonDSSWT


title: SC_microRNA_Cell_BaF3_R144

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R144


title: ColonDSSWT65

description: ColonDSSWT


title: ColonDSSTG48

description: ColonDSSTG


title: ColonBasalTG26

description: ColonBasalTG

value: Basal after the TG intestinal microbial transfer


title: SC_microRNA_Cell_BaF3_R146

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R146

value: Cell


title: SC_microRNA_Cell_BaF3_R148

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R148


title: ColonDSSWT55

description: ColonDSSWT


title: ColonBasalWT10

description: ColonBasalWT

value: Basal after the WT intestinal microbial transfer


title: ColonDSSWT56

description: ColonDSSWT


title: ColonBasalTG631

description: ColonBasalTG


title: ColonBasalTG28

description: ColonBasalTG


title: SC_microRNA_Cell_BaF3_R141

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R141


title: ColonBasalTG29

description: ColonBasalTG


title: SC_microRNA_Cell_BaF3_R142

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R142


title: ColonBasalWT7

description: ColonBasalWT


title: ColonBasalWT11

description: ColonBasalWT


title: SC_microRNA_Cell_BaF3_R139

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R139


title: SC_microRNA_Cell_BaF3_R138

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R138


title: ColonBasalTG31

description: ColonBasalTG


title: N2

city: Xi’an

first: Junlong

last: Zhao

state: Shaanxi

address: Chang-Le Xi Street #17

department: Department of Medical Genetics and Developmental Biology

institute: Fourth Military Medical University

data_processing:
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm8 whole genome using bowtie v0.12.2 with parameters -q -p 4 -e 100 -y -a -m 10 --best --strata
Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using a protocol from Chepelev et al., Nucleic Acids Research, 2009. In short, exons from all isoforms of a gene were merged to create one meta-transcript. The number of reads falling in the exons of this meta-transcript were counted and normalized by the size of the meta-transcript and by the size of the library.
Genome_build: mm8
Supplementary_files_format_and_content: tab-delimited text files include counts and RPKM values for each Sample

extract_protocol:
BM cells harvested from Ctrl or NICcA mice were stimulated with 25 ng/ml M-CSF (SinoBio) for 7 days to obtain BMDMs. RNA samples of above cells were extracted and sequencing was performed at RiboBio Co., Ltd (Guangzhou, China) with the Illumina HiSeq 3000.
RNA libraries were prepared for sequencing using standard Illumina protocols

tag: strain background

tag: genotype/variation

value: NICcA

tag: age

value: 7-8 weeks

value: bone marrow

value: bone marrow derived macrophages (BMDMs)


title: N3

tag: tissue


title: W3

value: wild type


title: ColonBasalWT8

description: ColonBasalWT


title: SC_microRNA_Cell_BaF3_R135

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R135


title: W2


title: W1


title: SC_microRNA_Cell_BaF3_R134

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R134


title: SC_microRNA_Cell_BaF3_R133

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R133


title: SC_microRNA_Cell_BaF3_R132

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R132


title: SC_microRNA_Cell_BaF3_R127

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R127


title: SC_mRNA_Cell_K562_R208

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R208

source_name: K562 single cell

growth_protocol: K562 cells were cultured in DMEM (Gibco) supplemented with 10% FBS (Gibco) and 1× GlutaMAX (Gibco).


title: SC_microRNA_Cell_BaF3_R129

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R129


title: SC_mRNA_Cell_K562_R205

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R205


title: SC_mRNA_Cell_K562_R204

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R204


title: SC_mRNA_Cell_K562_R203

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R203


title: SC_mRNA_Cell_K562_R206

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R206


title: SC_microRNA_Cell_BaF3_R131

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R131


title: SC_mRNA_Cell_K562_R207

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R207


title: SC_microRNA_Cell_BaF3_R126

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R126


title: SC_mRNA_Cell_K562_R202

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R202


title: SC_microRNA_Cell_BaF3_R130

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R130


title: ColonBasalWT9

description: ColonBasalWT


title: SC_mRNA_Cell_K562_R200

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R200


title: SC_mRNA_Cell_K562_R201

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R201


title: PolyIC-3

city: Paris

first: Masaya

last: OSHIMA

address: 123 boulevard de Port Royal

institute: INSERM U1016

description: PolyIC

hyb_protocol: After control of fragmentation using Bioanalyzer 2100, cDNA is then hybridized to GeneChip® Human Gene 2.0 ST (Affymetrix) at 45°C for 17 hours. After overnight hybridization, chips are washed on the fluidic station FS450 following specific protocols (Affymetrix).

scan_protocol: GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.

data_processing:
The scanned images are then analyzed with Expression Console software (Affymetrix) to obtain raw data (cel files) and metrics for Quality Controls. The observations of some of these metrics and the study of the distribution of raw data show no outlier experiment.
The data were analyzed with R Bioconductor packages using using RMA as normalization method

source_name: PolyIC, EndoC-βH1

label_protocol: Total RNA is reverse transcribed following the GeneChip™ WT PLUS Reagent Kit (applied biosystems). Briefly, the resulting double strand cDNA is used for amplification based on SPIA technology. After purification according to Nugen protocol, 3.6 ug of Sens Target DNA are fragmented and biotin labelled using Encore Biotin Module kit (Nugen).

extract_protocol: RNeasy Micro kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.

treatment_protocol: EndoC-ßH1 cells were transfected and sorted 48h post-transfection prior to RNA extraction

value: PolyIC transfected


title: Hypothalamus ICI 5 mg/kg/day infusion Replicate 5

first: Maria

middle: Belen

last: Rabaglino

address: Yalelaan 7

department: Population Health Science

institute: Utrecht University

description: Gene expression in fetal hypothalamus infused with 5 mg/kg/day of ICI 182,780-Replicate 5

hyb_protocol: 600 ng of Cy3-labelled cRNA (specific activity: 10.22 to 12.38 pmol Cy3/µg RNA) was fragmented and hybridized to Agilent Sheep Oligo Microarrays (G4813A) design 019921 by the ICBR facility at the University of Florida according to Agilent's methodology.

scan_protocol: Slides were scanned on the Agilent DNA Microarray Scanner (G2505B) using one color (green) scan setting for 8x15k array slides, by the ICBR facility at University of Florida.

data_processing: The scanned images were analyzed with Feature Extraction Software 10.1.1.1 (Agilent) using default parameters (protocol GE1-v5_10 and Grid: 019921_D_F_20100112)  background detrended Processed Signal. The limma package was employed to import the raw data into R (http://www.r-project.org), perform background correction and normalize the data by the quantile normalization method. Control probes and low expressed probes were filtered out, retaining for further analysis the probes that were at least 10% brighter than the negative controls on at least four arrays.

organism: Ovis aries

source_name: Fetal Hypothalamus, ICI 182,780 5 mg/kg/day infusion

label_protocol: Cyanine-3 (Cy3) labeled cRNA was prepared from 500 ng RNA using the One-Color Quick Amp Labeling Kit (Agilent, Newcastle DE) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA).  Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.

extract_protocol: RNA was extracted from fetal hyothalamus with Trizol (Life Technologies, Carlsbad, CA), and purified through Qiagen RNeasy+ kits with on-column DNase digestion (QIAGEN, Valencia, CA) according to manufacturers' protocols.Total RNA concentration was measured by Nanodrop ND-1000 and RNA quality was monitored by Agilent Bioanalyzer.

treatment_protocol: Chronically catheterized ovine fetuses were infused for 48 hours with: saline vehicle (Control, n=6), 17β-estradiol 500 ug/kg/day (Estradiol, n=4), ICI 182,780 5 μg/kg/day (ICI 5μg, n=4) and ICI 182,780 5mg/kg/day (ICI 5mg, n=5).

value: Fetal hypothalamus

tag: developmental age

value: Around 130 days of fetal age

value: ICI 182,780 5 mg/kg/day


title: SC_mRNA_Cell_K562_R199

first: Michael

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R199


title: PolyIC-1

description: PolyIC


title: PolyIC-2

description: PolyIC


title: CTRL-2

description: CTRL

source_name: CTRL, EndoC-βH1

value: CTRL


title: CTRL-1

description: CTRL


title: CTRL-3

description: CTRL


title: Hypothalamus ICI 5 mg/kg/day infusion Replicate 2

description: Gene expression in fetal hypothalamus infused with 5 mg/kg/day of ICI 182,780-Replicate 2


title: Hypothalamus ICI 5 mg/kg/day infusion Replicate 4

description: Gene expression in fetal hypothalamus infused with 5 mg/kg/day of ICI 182,780-Replicate 4


title: SC_microRNA_Cell_BaF3_R125

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R125


title: Hypothalamus ICI 5 mg/kg/day infusion Replicate 3

description: Gene expression in fetal hypothalamus infused with 5 mg/kg/day of ICI 182,780-Replicate 3


title: SC_mRNA_Cell_K562_R197

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R197


title: SC_microRNA_Cell_BaF3_R123

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R123


title: Hypothalamus ICI 5 mg/kg/day infusion Replicate 1

description: Gene expression in fetal hypothalamus infused with 5 mg/kg/day of ICI 182,780-Replicate 1


title: SC_mRNA_Cell_K562_R191

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R191


title: SC_mRNA_Cell_K562_R195

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R195


title: SC_microRNA_Cell_BaF3_R119

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R119


title: SC_mRNA_Cell_K562_R193

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R193


title: SC_mRNA_Cell_K562_R192

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R192


title: SC_microRNA_Cell_BaF3_R120

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R120


title: Hypothalamus ICI 5 μg/kg/day infusion Replicate 2

description: Gene expression in fetal hypothalamus infused with 5 μg/kg/day of ICI 182,780-Replicate 2

source_name: Fetal Hypothalamus, ICI 182,780 5 μg/kg/day infusion

value: ICI 182,780 5 μg/kg/day


title: SC_mRNA_Cell_K562_R190

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R190


title: SC_mRNA_Cell_K562_R198

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R198


title: SC_mRNA_Cell_K562_R187

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R187


title: SC_mRNA_Cell_K562_R189

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R189


title: Hypothalamus ICI 5 μg/kg/day infusion Replicate 1

description: Gene expression in fetal hypothalamus infused with 5 μg/kg/day of ICI 182,780-Replicate 1


title: Hypothalamus ICI 5 μg/kg/day infusion Replicate 4

description: Gene expression in fetal hypothalamus infused with 5 μg/kg/day of ICI 182,780-Replicate 4


title: SC_microRNA_Cell_BaF3_R118

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R118


title: Hypothalamus Estradiol 500 μg/kg/day Infusion Replicate 4

description: Gene expression in fetal hypothalamus infused with 500 μg/kg/day of Estradiol-Replicate 4

source_name: Fetal Hypothalamus, Estradiol 500 μg/kg/day Infusion

value: Estradiol 500 μg/kg/day


title: SC_mRNA_Cell_K562_R186

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R186


title: SC_microRNA_Cell_BaF3_R121

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R121


title: Hypothalamus Estradiol 500 μg/kg/day Infusion Replicate 3

description: Gene expression in fetal hypothalamus infused with 500 μg/kg/day of Estradiol-Replicate 3


title: Hypothalamus ICI 5 μg/kg/day infusion Replicate 3

description: Gene expression in fetal hypothalamus infused with 5 μg/kg/day of ICI 182,780-Replicate 3


title: SC_mRNA_Cell_K562_R188

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R188


title: SC_mRNA_Cell_K562_R181

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R181


title: SC_microRNA_NCC_BaF3_R117

description: This is a no-cell control (NCC) isolated from the BaF3 cell line. Chip cell processing unit R117

source_name: BaF3 no-cell control (NCC)

value: NCC


title: SC_mRNA_Cell_K562_R184

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R184


title: SC_mRNA_Cell_K562_R185

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R185


title: Hypothalamus Estradiol 500 μg/kg/day Infusion Replicate 2

description: Gene expression in fetal hypothalamus infused with 500 μg/kg/day of Estradiol-Replicate 2


title: SC_microRNA_Cell_BaF3_R116

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R116


title: SC_mRNA_Cell_K562_R180

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R180


title: SC_microRNA_Cell_BaF3_R114

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R114


title: SC_mRNA_Cell_K562_R183

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R183


title: SC_mRNA_Cell_K562_R182

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R182


title: SC_mRNA_Cell_K562_R179

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R179


title: SC_microRNA_Cell_BaF3_R113

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R113


title: Hypothalamus Estradiol 500 μg/kg/day Infusion Replicate 1

description: Gene expression in fetal hypothalamus infused with 500 μg/kg/day of Estradiol-Replicate 1


title: Hypothalamus Control-Fetal Infusion Replicate 6

description: Gene expression in fetal hypothalamus infused with vehicle-Replicate 6

source_name: Fetal Hypothalamus, Fetal vehicle infusion

value: vehicle


title: SC_mRNA_Cell_K562_R176

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R176


title: SC_microRNA_Cell_BaF3_R111

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R111


title: SC_microRNA_Cell_BaF3_R110

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R110


title: SC_mRNA_Cell_K562_R172

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R172


title: SC_mRNA_Cell_K562_R177

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R177


title: SC_mRNA_Cell_K562_R171

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R171


title: Hypothalamus Control-Fetal Infusion Replicate 4

description: Gene expression in fetal hypothalamus infused with vehicle-Replicate 4


title: SC_mRNA_Cell_K562_R170

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R170


title: SC_mRNA_Cell_K562_R178

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R178


title: SC_mRNA_Cell_K562_R169

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R169


title: Hypothalamus Control-Fetal Infusion Replicate 3

description: Gene expression in fetal hypothalamus infused with vehicle-Replicate 3


title: SC_microRNA_Cell_BaF3_R109

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R109


title: SC_microRNA_Cell_BaF3_R108

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R108


title: SC_mRNA_Cell_K562_R166

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R166


title: SC_mRNA_Cell_K562_R163

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R163


title: SC_mRNA_Cell_K562_R173

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R173


title: SC_mRNA_Cell_K562_R174

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R174


title: SC_microRNA_Cell_BaF3_R106

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R106


title: SC_mRNA_Cell_K562_R167

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R167


title: SC_microRNA_Cell_BaF3_R107

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R107


title: SC_mRNA_Cell_K562_R165

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R165


title: SC_microRNA_Cell_K562_R102

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R102


title: SC_microRNA_Cell_K562_R103

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R103


title: SC_mRNA_Cell_K562_R162

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R162


title: Hypothalamus Control-Fetal Infusion Replicate 1

description: Gene expression in fetal hypothalamus infused with vehicle-Replicate 1


title: Hypothalamus Control-Fetal Infusion Replicate 2

description: Gene expression in fetal hypothalamus infused with vehicle-Replicate 2


title: SC_microRNA_Cell_BaF3_R112

description: This is a single cell isolated from the BaF3 cell line. Chip cell processing unit R112


title: SC_microRNA_Cell_K562_R104

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R104


title: SC_microRNA_Cell_K562_R100

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R100


title: Hypothalamus Control-Fetal Infusion Replicate 5

description: Gene expression in fetal hypothalamus infused with vehicle-Replicate 5


title: SC_mRNA_Cell_K562_R155

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R155


title: SC_mRNA_Cell_K562_R153

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R153


title: SC_mRNA_Cell_K562_R154

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R154


title: SC_mRNA_Cell_K562_R158

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R158


title: SC_mRNA_Cell_K562_R159

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R159


title: SC_microRNA_Cell_K562_R99

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R99


title: SC_mRNA_Cell_K562_R168

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R168


title: SC_mRNA_Cell_K562_R164

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R164


title: SC_mRNA_Cell_K562_R157

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R157


title: SC_mRNA_Cell_K562_R151

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R151


title: SC_microRNA_Cell_K562_R98

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R98


title: SC_mRNA_Cell_K562_R156

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R156


title: SC_mRNA_Cell_K562_R148

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R148


title: SC_mRNA_Cell_K562_R152

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R152


title: SC_microRNA_Cell_K562_R101

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R101


title: SC_mRNA_Cell_K562_R160

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R160


title: SC_mRNA_Cell_K562_R147

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R147


title: SC_mRNA_Cell_K562_R149

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R149


title: SC_mRNA_Cell_K562_R150

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R150


title: SC_mRNA_Cell_K562_R146

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R146


title: SC_mRNA_Cell_K562_R145

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R145


title: SC_mRNA_Cell_K562_R144

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R144


title: SC_microRNA_Cell_K562_R94

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R94


title: SC_microRNA_Cell_K562_R96

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R96


title: SC_microRNA_Cell_K562_R95

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R95


title: SC_mRNA_Cell_K562_R143

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R143


title: SC_mRNA_Cell_K562_R142

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R142


title: SC_microRNA_Cell_K562_R93

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R93


title: SC_mRNA_Cell_K562_R141

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R141


title: SC_mRNA_Cell_K562_R138

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R138


title: SC_mRNA_Cell_K562_R140

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R140


title: SC_microRNA_Cell_K562_R91

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R91


title: SC_mRNA_Cell_K562_R134

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R134


title: SC_microRNA_Cell_K562_R90

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R90


title: SC_mRNA_Cell_K562_R133

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R133


title: SC_microRNA_Cell_K562_R97

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R97


title: SC_mRNA_Cell_K562_R139

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R139


title: SC_mRNA_Cell_K562_R130

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R130


title: SC_microRNA_Cell_K562_R89

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R89


title: SC_mRNA_Cell_K562_R128

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R128


title: SC_mRNA_Cell_K562_R136

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R136


title: SC_mRNA_Cell_K562_R127

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R127


title: SC_microRNA_Cell_K562_R88

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R88


title: SC_mRNA_Cell_K562_R132

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R132


title: SC_mRNA_Cell_K562_R126

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R126


title: SC_mRNA_Cell_K562_R131

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R131


title: SC_microRNA_Cell_K562_R92

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R92


title: SC_mRNA_Cell_K562_R124

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R124


title: SC_microRNA_Cell_K562_R87

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R87


title: SC_microRNA_Cell_K562_R85

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R85


title: SC_mRNA_Cell_K562_R125

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R125


title: SC_mRNA_Cell_K562_R103

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R103


title: SC_microRNA_Cell_K562_R86

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R86


title: SC_microRNA_Cell_K562_R84

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R84


title: SC_mRNA_NCC_K562_R118

description: This is a no-cell control (NCC) isolated from the K562 cell line. Chip cell processing unit R118

source_name: K562 no-cell control (NCC)


title: SC_mRNA_NCC_K562_R112

description: This is a no-cell control (NCC) isolated from the K562 cell line. Chip cell processing unit R112


title: SC_mRNA_Cell_K562_R129

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R129


title: SC_mRNA_Cell_K562_R99

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R99


title: SC_mRNA_Cell_K562_R96

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R96


title: SC_mRNA_Cell_K562_R97

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R97


title: SC_mRNA_Cell_K562_R94

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R94


title: SC_microRNA_Cell_K562_R81

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R81


title: SC_microRNA_Cell_K562_R80

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R80


title: SC_mRNA_Cell_K562_R101

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R101


title: SC_mRNA_Cell_K562_R102

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R102


title: SC_mRNA_Cell_K562_R135

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R135


title: SC_microRNA_Cell_K562_R79

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R79


title: SC_microRNA_Cell_K562_R78

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R78


title: SC_mRNA_Cell_K562_R88

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R88


title: SC_microRNA_Cell_K562_R83

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R83


title: SC_mRNA_Cell_K562_R98

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R98


title: SC_mRNA_Cell_K562_R87

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R87


title: SC_microRNA_Cell_K562_R75

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R75


title: SC_mRNA_Cell_K562_R93

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R93


title: SC_mRNA_Cell_K562_R92

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R92


title: SC_mRNA_Cell_K562_R89

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R89


title: SC_mRNA_Cell_K562_R83


title: SC_mRNA_Cell_K562_R90

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R90


title: SC_mRNA_Cell_K562_R91

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R91


title: SC_microRNA_Cell_K562_R74

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R74


title: SC_mRNA_Cell_K562_R86

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R86


title: SC_microRNA_Cell_K562_R69

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R69


title: SC_microRNA_Cell_K562_R70

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R70


title: SC_mRNA_Cell_K562_R84

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R84


title: SC_mRNA_Cell_K562_R82

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R82


title: SC_microRNA_Cell_K562_R76

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R76


title: SC_mRNA_Cell_K562_R85

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R85


title: SC_mRNA_Cell_K562_R75


title: SC_mRNA_Cell_K562_R81

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R81


title: SC_mRNA_Cell_K562_R74


title: SC_microRNA_Cell_K562_R66

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R66


title: SC_microRNA_Cell_K562_R73

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R73


title: SC_mRNA_Cell_K562_R80

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R80


title: SC_mRNA_Cell_K562_R79

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R79


title: SC_mRNA_Cell_K562_R78

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R78


title: SC_microRNA_Cell_K562_R65

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R65


title: SC_mRNA_Cell_K562_R65


title: SC_microRNA_Cell_K562_R64

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R64


title: SC_microRNA_Cell_K562_R62

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R62


title: SC_microRNA_Cell_K562_R71

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R71


title: SC_mRNA_Cell_K562_R69


title: SC_mRNA_Cell_K562_R72

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R72


title: SC_microRNA_Cell_K562_R68

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R68


title: SC_mRNA_Cell_K562_R73


title: SC_mRNA_Cell_K562_R71


title: SC_mRNA_Cell_K562_R77

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R77


title: SC_mRNA_Cell_K562_R70

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R70


title: SC_mRNA_Cell_K562_R58

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R58


title: SC_mRNA_Cell_K562_R59

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R59


title: SC_mRNA_Cell_K562_R60

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R60


title: SC_microRNA_Cell_K562_R60


title: SC_microRNA_Cell_K562_R67

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R67


title: SC_mRNA_Cell_K562_R76

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R76


title: SC_mRNA_Cell_K562_R56

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R56


title: SC_mRNA_Cell_K562_R57

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R57


title: SC_mRNA_Cell_K562_R62


title: SC_mRNA_Cell_K562_R64


title: SC_mRNA_Cell_K562_R66

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R66


title: SC_microRNA_Cell_K562_R58


title: SC_mRNA_Cell_K562_R63

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R63


title: SC_mRNA_Cell_K562_R68


title: SC_mRNA_Cell_K562_R61

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R61


title: SC_microRNA_Cell_K562_R51

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R51


title: SC_mRNA_Cell_K562_R67


title: SC_mRNA_Cell_K562_R55

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R55


title: SC_mRNA_Cell_K562_R40

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R40


title: SC_microRNA_Cell_K562_R61


title: SC_mRNA_Cell_K562_R39

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R39


title: SC_mRNA_Cell_K562_R38

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R38


title: SC_microRNA_Cell_K562_R63


title: SC_mRNA_Cell_K562_R52

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R52


title: SC_microRNA_Cell_K562_R57


title: SC_mRNA_Cell_K562_R50

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R50


title: SC_mRNA_Cell_K562_R51


title: SC_microRNA_Cell_K562_R56


title: SC_mRNA_Cell_K562_R49

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R49


title: SC_microRNA_Cell_K562_R59

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R59


title: SC_microRNA_Cell_K562_R54

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R54


title: SC_microRNA_Cell_K562_R49


title: SC_mRNA_Cell_K562_R47

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R47


title: SC_mRNA_Cell_K562_R46

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R46


title: SC_mRNA_Cell_K562_R45

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R45


title: SC_mRNA_Cell_K562_R43

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R43


title: SC_mRNA_Cell_K562_R48

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R48


title: SC_mRNA_Cell_K562_R35

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R35


title: SC_mRNA_Cell_K562_R33

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R33


title: SC_mRNA_Cell_K562_R32

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R32


title: SC_microRNA_Cell_K562_R50


title: SC_mRNA_Cell_K562_R44

description: This is a single cell isolated from the K562 cell line. Chip cell processing unit R44


title: SC_microRNA_Cell_K562_R52