title: siTP53 ctr rep2

description:
untreated control siTP53-transfected-transfected cells
NG-7926_ctr_2_si_p53_lib80457_3949_6_1


title: EC7

status: Public on Feb 16 2018

type: SRA

city: New York

first: Aarthi

last: Ramakrishnan

state: New York

address: 1425 Madison Avenue

department: Neuroscience

country: USA

institute: Icahn School of Medicine at Mount Sinai

library_source: transcriptomic

data_processing:
Illumina Casava1.7 software used for basecalling.
Data from mice were aligned to the mm9 reference transcriptome using Tophat, and analyzed using voom and limma packages to generate differential expression signatures
Genome_build: mm9
Supplementary_files_format_and_content: Counts in tab delimited text files generated using HTSeq-count against Ensembl v67 annotation

molecule: polyA RNA

organism: Mus musculus

source_name: Brain

extract_protocol:
Total RNA was isolated from frozen dissected NAc tissue using QIAzol lysis reagent and purified using the RNAeasy mini kit (Qiagen). RNA integrity was assayed using an Aligent 2100 Bioanalyzer (Aligent, Santa Clara CA).
Libraries were prepared using the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego CA).

tag: region

value: NAc

tag: condition

value: Control

tag: genotype

value: ESR1 OE

tag: Sex

value: Male


title: EC8


title: EC6


title: EC4


title: EC5


title: CG8

value: GFP

value: Female


title: human colorectal tumor tissue, patient sample 24

status: Public on Feb 17 2018

type: RNA

city: Guangzhou

first: Huanliang

last: Liu

address: 26 Yuancun Er Heng Road

country: China

institute: Guangdong Institute of Gastroenterology and the Sixth Affiliated Hospital, Sun Yat-sen University

description: LncRNA and gene expression in colon cancer tissue

hyb_protocol: 600ng of Cy3-labelled linearly amplified aRNA was fragmented at 60°C for 30 minutes in a reaction volume of 25 ul containing 25x Agilent fragmentation buffer and 10x Agilent blocking agent following the manufacturers instructions. On completion of the fragmentation reaction, 25 ul of 2x GEx Hybridization Buffer HI-RPM was added to the fragmentation mixture and hybridized for 17 hours at 65°C in a rotating Agilent hybridization oven.  After hybridization, microarrays were washed 1 minute at room temperature with Gene Expression Wash Buffer 1  (Agilent) and 1 minute with 37°C Gene Expression Wash Buffer 2  (Agilent), then dried immediately by brief centrifugation.

scan_protocol: Slides were scanned immediately after washing on the Agilent Microarray Scanner (G2565CA) using one color scan setting for 4x180k array slides.

data_processing: The scanned images were analyzed with Feature Extraction Software 9.1 (Agilent) using default parameters (protocol GE1-v1_91 and Grid: 012391_D_20060331) to obtain background subtracted and spatially detrended Processed Signal intensities.  Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.

molecule: total RNA

organism: Homo sapiens

source_name: tumor tissue

label_protocol: Cyanine-3 (Cy3) labeled cRNA was prepared from 0.5 ug RNA using the One-Color Low RNA Input Linear Amplification PLUS kit (Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA).  Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.

extract_protocol: RNA was prepared using the RNeasy mini kit (Qiagen, GmBH, Germany) following the manufacturer's recommendations.  RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).

treatment_protocol: Fresh samples obtained from surgery were protected in RNAlater and kept in liquid nitrogen.

tag: tissue

value: tumor tissue

tag: age

tag: patient diagnosis

value: colon cancer

tag: gender

value: male


title: EC3


title: EC2


title: CG9


title: CG7


title: p28 Can_1

city: Bonn

first: Joachim

last: Schultze

state: NRW

address: Carl-Troll-Strasse 31

department: Genomics and Immunoregulation

country: Germany

institute: LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)

description: p28 Can

hyb_protocol: Standard Illumina hybridization protocol

scan_protocol: Standard Illumina scanning protocol

data_processing: The data were normalised using quantile normalisation in Partek Genomic Suite

label: biotin

source_name: Hippocampus

label_protocol: Biotinylated cRNA were prepared with the TargetAmpTM Nano-gTM Biotin-aRNA Labeling Kit

extract_protocol: Total RNA from all cell samples was isolated using TRIzol Reagent (Invitrogen).

tag: strain

value: Hippocampus


title: human colorectal normal tissue, patient sample 17

description: LncRNA and gene expression in rectal paraneoplastic tissue

source_name: adjacent non-tumor tissue

value: adjacent non-tumor tissue

value: rectal cancer

value: female


title: CG5


title: adult Can_1

description: adult Can


title: human colorectal tumor tissue, patient sample 17

description: LncRNA and gene expression in rectal cancer tissue


title: CG4


title: CE5


title: CE6


title: CE4


title: CE9


title: CE2


title: CG10


title: CG2


title: CE3


title: im-scWAT_D8

city: Tokyo

first: Juro

last: Sakai

address: 4-6-1, Komaba, Meguro-ku

department: Division of Metabolic Medicine

country: Japan

institute: Research Center for Advanced Science and Technology, The University of Tokyo

data_processing:
The libraries were sequenced on HiSeq 2500 (Illumina), and the reads were aligned to mouse transcriptome (UCSC gene) and genome (mm9) references respectively using Burrows-Wheeler Aligner.
After transcript coordinate was converted to genomic positions, an optimal mapping result was selected either from transcript or genome mapping by comparing the minimal edit distance to the reference.
Local realignment was performed within in-house short reads aligner with smaller k-mer size (k=11).
Finally, fragments per kilobase of exon per million fragment mapped (FPKM) values were calculated for each UCSC gene while considering strand-specific information.
Genome_build: mm9
Supplementary_files_format_and_content: Tab-delimited text files include FPKM values for each sample.

source_name: im-scWAT

growth_protocol: Immortalized preadipocytes derived from inguinal subcutaneous white adipose tissue (im-scWAT) were cultured in the complete medium (DMEM/F12 containing 1.2 g/L NaHCO3 and 10% FBS)  at 37 °C.

extract_protocol:
Total RNA was extracted using ISOGEN (Nippongene) following manufacturer's protocol.
Libraries were prepared by TruSeq sample purification kit (Illumina) following manufacturer's protocol.

treatment_protocol: Preadipocytes were infected with retroviruses for shRNA targeting mJmjd1a and for wild-type and mutant versions of hJMJD1A. Preadipocytes cultured to ~100% confluence were induced with induction medium (i.e. complete medium containing 0.125 mM indomethacin, 5 μM dexamethasone, 0.5 mM 3-isobutyl-1-methylxanthine and 0.5 μM rosiglitazone, 5 μg/ml insulin, 1 nM T3) (Day 0). After 2 days, cells were incubated in the maintenance medium with 0.5 μM rosiglitazone (i.e. complete medium containing only insulin, T3 and rosiglitazone) for another 5 days. On day 7, unless otherwise stated, medium was switched to maintenance medium without rosiglitazone (i.e. complete medium containing only insulin and T3) and cultured another 1 day before the assay (Day8). For preparing "rosiglitazone minus" samples, rosiglitazone was not added in neither the induction medium or the maintenance medium during entire process of differentiation.

tag: cell type

value: immortalized preadipocytes derived from inguinal subcutaneous white adipose tissue (im-scWAT)

tag: rosiglitazone

value: plus

tag: time point

value: Day 8


title: im-scWAT_D8_PPARγ

library_source: genomic

data_processing:
The libraries were sequenced on HiSeq 2500 (Illumina), and all bound DNA fragments were mapped to UCSC build mm9 assembly of the mouse genome by the mapping program ELAND based on the 36 bp sequences.
The mapped sequence reads were extended to 500 bp, normalized by million mapped reads, and converted to the continuous signal data in wig format.
Wig files were converted to bigwig files using UCSC wigToBigWig program.
Genome_build: mm9
Supplementary_files_format_and_content: bigWig files

molecule: genomic DNA

extract_protocol:
Sonicated nuclear fraction was incubated with each antibody and JMJD1A-DNA or PPARγ-DNA complexes were isolated.
Libraries were prepared by TruSeq sample purification kit (Illumina) following manufacturer's protocol.

treatment_protocol: Preadipocytes cultured to ~100% confluence were induced with induction medium (i.e. complete medium containing 0.125 mM indomethacin, 5 μM dexamethasone, 0.5 mM 3-isobutyl-1-methylxanthine and 0.5 μM rosiglitazone, 5 μg/ml insulin, 1 nM T3) (Day 0). After 2 days, cells were incubated in the maintenance medium with 0.5 μM rosiglitazone (i.e. complete medium containing only insulin, T3 and rosiglitazone) for another 5 days. On day 7, unless otherwise stated, medium was switched to maintenance medium without rosiglitazone (i.e. complete medium containing only insulin and T3) and cultured another 1 day before the assay (Day8).

tag: day

tag: chip antibody

value: PPARγ

tag: chip antibody info.

value: mix of Santa Cruz, sc-7273, lot 10513 and lab-generated IgG-A3409A


title: im-scWAT_D8_sh-mJmjd1a_WT-hJMJD1A_repB

tag: shRNA

value: Wild-type


title: im-scWAT_D8_sh-mJmjd1a_WT-hJMJD1A_repA


title: im-scWAT_D4_Rosi_minus

value: minus

value: Day 4


title: im-scWAT_D0

value: Day 0


title: im-scWAT_D4


title: im-scWAT_D8_Rosi_minus


title: im-scWAT_D8_sh-mJmjd1a_S265D-H1120Y-hJMJD1A


title: CG3

value: NAc

value: Control

value: GFP

value: Female


title: im-scWAT_D8_sh-mJmjd1a_S265A-hJMJD1A


title: im-scWAT_D8_sh-mJmjd1a_S265D-hJMJD1A


title: im-scWAT_D0_Rosi_minus


title: im-scWAT_D8_JMJD1A

value: mix of two lab-generated  IgG-F0231 (lot A-2) and IgG-F0618 (lot A-2)


title: TBI+GPX2 virus [GPX2_3]

city: Northbrook

first: Michael

last: Falduto

state: IL

address: 1808 Janke, Unit M

institute: GenUs BioSystems, Inc.

hyb_protocol: 10 µg of purified cRNA was fragmented to uniform size and applied to CodeLink Rat Whole Genome Microarrays in hybridization buffer. Arrays were hybridized at 37° C for 18 hrs. in a shaking incubator, and washed at 46° C for 1 hr.

scan_protocol: Rinsed and dried arrays were scanned with an Axon GenePix 4000B Microarray Scanner at 5 µm resolution.

data_processing: CodeLink software was used to process the scanned images from arrays (gridding and feature intensity extraction) and the data generated for each probe on the array was analyzed with GeneSpring GX v7.3.1 software (Agilent Technologies, Santa Clara, CA).

label: Alexa 555

organism: Rattus norvegicus

source_name: Hippocampus LCM Neurons, traumatic brain injury, GPX2 knockdown, 2wk, pool from 3 animals

label_protocol: 2 rounds of amplification were performed using the Arcturus RiboAmp HS kit.  Biotinylated cRNA target was prepared from the DNA template purified after the  second round cDNA synthesis.  cRNA quality was verified on an Agilent Bioanalyzer  cRNA was fragmented to uniform size and verified on the Bioanalyzer.

extract_protocol: Total RNA from laser captured hippocampal neurons was isolated and DNase treated prior to microarray analysis.

treatment_protocol:
All AAV viral constructs were produced by Vector Biolabs.  3 siRNA sequences targeted to either nNOS or GPX-1 were cloned into the AAV2/1 viral backbone. siRNA oligos were purchased from Ambion and were tested in a neuronal cell culture model to verify gene expression knockdown before providing Vector Biolabs with the sequences for virus production.
Rats received either sham injury, TBI, or  TBI + AAV virus (scrambled control virus or nNOS or GPx-1 siRNA constructs) which were stereotaxically injected into the CA1/2 and CA3 regions of the hippocampus.  Rats were survived for 15 days, sacrificed, brains were removed and frozen on dry ice.  Coronal sections of the brain were collected on glass slides and pyramidal neurons from the CA1/2 and CA3 regions of the hippocampus that expressed the AAV virus were collected by laser capture microdissection.

value: Sprague-Dawley

tag: received

value: traumatic brain injury

tag: treated with

value: GPX2 knockdown siRNA contruct

value: 2 weeks post-treatment

value: Hippocampus LCM Neurons


title: TBI+nNos3 virus [nNos3_2]

source_name: Hippocampus LCM Neurons, traumatic brain injury, nNos3 knockdown, 2wk, pool from 3 animals

value: nNos3 knockdown siRNA construct


title: TBI+GPX2 virus [GPX2_2]


title: TBI+nNos3 virus [nNos3_1]


title: TBI+GPX2 virus [GPX2_1]


title: TBI+Scramble virus control [scr_3]

source_name: Hippocampus LCM Neurons, traumatic brain injury, Scramble virus control, 2wk, pool from 3 animals

value: scramble virus control


title: TBI+Scramble virus control [scr_2]


title: TBI+Scramble virus control [scr_1]


title: TBI alone control [TBI_3]

source_name: Hippocampus LCM Neurons, traumatic brain injury, Saline, 2wk, pool from 3 animals

value: saline


title: TBI alone control [TBI_2]


title: TBI alone control [TBI_1]


title: Sham control baseline [Sham_2]

source_name: Hippocampus LCM Neurons, sham injury, 2wk, pool from 3 animals

value: sham injury


title: Sham control baseline [Sham_1]


title: TBI+nNos3 virus [nNos3_3]


title: Sham control baseline [Sham_3]


title: Skin_SSR_No200KVitD_rep2

city: Cleveland

first: Kurt

middle: Q

last: Lu

state: OH

address: 2109 Adelbert Road

department: Dermatology

institute: Case Western Reserve University/ University Hospitals Case Medical Ctr

description: Gene expression data  from irradiated skin tissue without treatment

hyb_protocol: Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C while on rotation (60rpm) on Affymetrix Human Gene 2.0 ST Arrays.

scan_protocol: GeneChips were scanned using Affymetrix' GeneChip Scanner 3000.

data_processing: The data were analyzed with Rv3.2.2/Bioconductor. The oligo package was used to read, background correct, and normalize the raw sample data using the Robust Multiarray Average algorithm.

label: Biotin

source_name: Inner arm skin, SSR, no vitamin D given 1 hour after irradiation, biopsy at 48 hrs

label_protocol: Biotinylated cRNA were prepared using Affymetrix' WT plus labeling kit.

extract_protocol: RNA was extracted from fresh frozen punch biopsies using the RNeasy Lipid Mini Kit (Qiagen, Redwood City, CA)

treatment_protocol: A 6 mm punch biopsy specimen was obtained from the 3MED site 48hr after irradiation.

value: Skin

value: M

tag: ethnicity

value: Non-hispanic/Latino


title: Skin_SSR_No200KVitD_rep4


title: Skin_SSR_No200KVitD_rep3


title: Skin_SSR_100KVitD_rep4

description: Gene expression data from irradiated tissue with treatment

source_name: Inner arm skin, SSR, 100,000IU VitaminD given 1 hour after irradiation, biopsy at 48 hrs


title: Skin_SSR_200KVitD_rep4

source_name: Inner arm skin, SSR, 200,000IU VitaminD given 1 hour after irradiation, biopsy at 48 hrs


title: Skin_SSR_200KVitD_rep5


title: Skin_SSR_No100KVitD_rep4


title: Skin_SSR_200KVitD_rep3


title: Skin_SSR_100KVitD_rep3

value: F


title: Skin_SSR_No200KVitD_rep1


title: Skin_SSR_No200KVitD_rep5


title: Skin_SSR_200KVitD_rep2


title: Skin_SSR_200KVitD_rep1


title: Skin_SSR_No100KVitD_rep3


title: siTP53 ctr rep3

city: Bergen

first: Tomasz

last: Stokowy

address: Nygardsgaten 5

department: IT Division

country: Norway

institute: University of Bergen

description:
untreated control siTP53-transfected-transfected cells
NG-7926_ctr_3_si_p53_lib80476_4027_5_1

data_processing:
alignment to hg19, hisat 2.0.5
feature counting, featureCounts
normalization, DESeq2
Genome_build: hg19
Supplementary_files_format_and_content: txt file, normalized expression values

source_name: U2OS cells

growth_protocol: The wild-type U2-OS cells were purchased from ATCC (HTB-96) and used as a primary model. Cells were grown in McCoy’s 5A medium supplemented with 10% heat-inactivated fetal bovine serum (HyClone) and gentamicin (40 mg/mL; Krka) at 37 °C in a humidified 5% CO2 atmosphere.

extract_protocol:
Total RNA was extracted from 1x106 cells using RNeasy Mini Kit (Qiagen) and treated on-column with DNase using the RNase-Free DNase Set (Qiagen).
RNA libraries were prepared for sequencing using 1ug RNA according to the standard Illumina protocols

treatment_protocol: For expression analysis wild type and transfected cells were used. Cells were transiently transfected with 10 nM siRNA (Eurofins) using INTERFERin® Kit (Polyplus Transfection) for 48 hours using sequences specific for TP53 gene, RELA gene, or control siRNA. Any treatment started 48 h after inoculation of cells (wild type cells) or after 48h of siRNA transfection (transfected cells); all steps, with the exception of irradiation, were carried out at 37 °C and 5% CO2. For gene expression analysis cells were irradiated with 4 or 10 Gy IR dose at a 1Gy/min dose rate using 6 MeV photons (generated by linear accelerator Clinac 600, Varian), the medium was replaced with a fresh one, and then cells were harvested 4 hours after irradiation.

tag: tumor

value: osteosarcoma

value: female

value: 15 years

tag: tissue/cells

value: bone


title: Skin_SSR_No100KVitD_rep2

tag: tissue


title: Skin_SSR_100KVitD_rep1

tag: age


title: Skin_SSR_100KVitD_rep2


title: Skin_SSR_50KVitD_rep5

source_name: Inner arm skin, SSR, 50,000IU VitaminD given 1 hour after irradiation, biopsy at 48 hrs


title: Skin_SSR_No100KVitD_rep1


title: Skin_SSR_No50KVitD_rep5


title: Skin_SSR_50KVitD_rep4


title: Skin_SSR_No50KVitD_rep4


title: Skin_SSR_No50KVitD_rep1


title: Skin_SSR_Noplacebo_rep4

source_name: Inner arm skin, SSR, no placebo given 1 hour after irradiation, biopsy at 48 hrs


title: Skin_SSR_50KVitD_rep1


title: Skin_SSR_Placebo_rep2

source_name: Inner arm skin, SSR, placebo given 1 hour after irradiation, biopsy at 48 hrs

value: Hispanic/Latino


title: Skin_SSR_No50KVitD_rep2


title: Skin_SSR_50KVitD_rep3


title: Skin_SSR_Noplacebo_rep3


title: Skin_SSR_Placebo_rep1


title: Skin_SSR_No50KVitD_rep3


title: Skin_SSR_Noplacebo_rep2


title: Skin_NoSSRcontrol_rep5

description: Gene expression data  from unirradiated skin tissue (control)

source_name: Inner arm skin, No SSR


title: Skin_SSR_50KVitD_rep2


title: Skin_NoSSRcontrol_rep1


title: Skin_SSR_Noplacebo_rep1


title: Skin_NoSSRcontrol_rep4


title: Skin_NoSSRcontrol_rep2


title: DHT-rep3

city: Ann Arbor

first: Marcin

middle: Piotr

last: Cieslik

state: Michigan

address: 500 S State St

department: Pathology

institute: University of Michigan

description: DHT treatment of MDA-PCa-2b

hyb_protocol: Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequentially.

scan_protocol: Scanned on an Agilent G2505B scanner. Images were quantified using Agilent Feature Extraction Software (version A.8.5.1.1).

data_processing: Agilent Feature Extraction Software (v 8.5.1.1) was used for background subtraction and LOWESS normalization. Control Features were omitted. The LogRatio columns from each feature file were used for further analyses.

source_name: DHT treated MDA-PCa-2b cells

label_protocol: 10 ug of total RNA were primed with 2 ul of 100 uM T16N2 DNA primer at 70_C for 10 min, then reversed transcribed at 42_C for 1 h in the presence of 400 U SuperScript II RTase (Invitrogen), and 100 uM each dATP, dTTP, dGTP, with 25 uM dCTP, 25 uM Cy3-label.

growth_protocol: MDA-PCa-2b cells were maintained in ATCC-formulated F-12K medium, supplemented with 20% FBS, 25 ng/ml cholera toxin, 10 ng/ml mouse epidermal growth factor, 100 pg/ml hydrocortisone, 0.005 mM phosphoethanolamine, 45nM selenous acid, and 0.005 mg/ml bovine insulin.

extract_protocol: Total RNA extracted using Qiazol and miRNeasy kit (QIAGEN) following manufacturer's instructions.

treatment_protocol: For transfection, MDA-PCa-2b cells were transfected with indicated siRNAs or ASOs using lipofectamine RNAiMAX according to the manufacturer’s instructions. RNA was harvested for analysis 48 hours after transfection. For DHT treatment, MDA-PCa-2b cells were serum-starved for 48 hours before treated with 10nM DHT (or Vehcle) for 12 hours. RNA was then harvested for microarray analysis.

tag: cell line

tag: agent

value: DHT

source_name: Vehicle treated MDA-PCa-2b cells

value: control


title: DHT-rep2

description: DHT treatment of MDA-PCa-2b


title: ARlnc1-aso6-rep1

description: ASO kd of ARlinc1

source_name: Control ASO transfected MDA-PCa-2b cells

tag: transfection

value: Control ASO

source_name: ARlinc1 ASO transfected MDA-PCa-2b

value: ARlinc1 ASO6


title: Skin_NoSSRcontrol_rep3

tag: gender


title: Skin_SSR_Placebo_rep3


title: ARlnc1-aso6-rep2

description: ASO kd of ARlinc1


title: ARlnc1-aso2-rep2

description: ASO kd of ARlinc1

value: ARlinc1 ASO2


title: ARlnc1-aso2-rep1

description: ASO kd of ARlinc1


title: DHT-rep1

description: DHT treatment of MDA-PCa-2b


title: ARlnc1-si3-rep3

description: siRNA kd of ARlnc1

source_name: Non-targeting siRNA transfected MDA-PCa-2b cells

value: Non-targeting siRNA

source_name: ARlnc1 siRNA transfected MDA-PCa-2b cells

value: ARlnc1 siRNA3


title: BIPR_4H_Treated, biological repA

city: University

first: Ameeta

last: Agarwal

state: MS

address: NCNPR, Room 2049

department: National Center for Natural Products Research

institute: University of Mississippi

description: Gene expression data from yeast cells treated with BIPR for 4h (repA)

hyb_protocol: Affymetrix GeneChip Hybridization protocol, and GeneChip Fluidics Station 450 default protocol were used. GeneChip Yeast Genome 2.0 arrays were used.

scan_protocol: Affymetrix GenChip Scanner 3000 default parameters were used.

data_processing: Image analysis, scaling (TGT = 1000), and probeset-level data analysis was performed using default parameters in Affymetrix software.

organism: Saccharomyces cerevisiae

source_name: Yeast cells treated with BIPR for 4H

label_protocol: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.

growth_protocol: All cultures were grown in SD broth (2% dextrose, 0.67% yeast nitrogen base without amino acids, MOPS buffered, pH 7.0) at 30 degrees, with shaking at 200 rpm.

extract_protocol: Qiagen RNeasy Midi kit was used for total RNA extraction.

treatment_protocol: Cultures of S. cerevisiae strain S288C were started at OD600 of 0.1, allowed to grow to OD600 of 0.2, then treated with either DMSO (0.25%) or specific compounds at their respective IC50 concentrations.  When OD600 of 0.5 was reached, cells were harvested and frozen. For the long-term exposure experiment, all experimental conditions were the same except cells were exposed to compound treatments for 4.5 doublings (~ 15 h). In all experiments, three biological replicate cultures were grown for each treatment, and RNA from each replicate was hybridized to an independent array.

tag: strain

tag: treatment duration (hours)


title: BIPR_4H_Treated, biological repC

description: Gene expression data from yeast cells treated with BIPR for 4h (repC)


title: ARlnc1-si2-rep2

description: siRNA kd of ARlnc1

value: ARlnc1 siRNA2


title: BIPRandPHEN_4H_DMSO_Control, biological repC

description: Gene expression data from yeast cells treated with DMSO for 4h (repC)

source_name: Yeast cells treated with DMSO for 4H


title: PHEN_4H_Treated, biological repC

description: Gene expression data from yeast cells treated with PHEN for 4h (repC)

source_name: Yeast cells treated with PHEN for 4H


title: ARlnc1-si3-rep1

description: siRNA kd of ARlnc1


title: ARlnc1-si2-rep3

description: siRNA kd of ARlnc1


title: ARlnc1-si3-rep2

description: siRNA kd of ARlnc1


title: ARlnc1-si2-rep1

description: siRNA kd of ARlnc1


title: PHEN_4H_Treated, biological repB

description: Gene expression data from yeast cells treated with PHEN for 4h (repB)


title: Skin_SSR_Placebo_rep4

value: Skin


title: PHEN_4H_Treated, biological repA

description: Gene expression data from yeast cells treated with PHEN for 4h (repA)


title: BIPR_4H_Treated, biological repB

description: Gene expression data from yeast cells treated with BIPR for 4h (repB)


title: LMT_15H_Treated, biological repC

description: Gene expression data from yeast cells treated with LMT for 15h (repC)

source_name: Yeast cells treated with LMT for 15H


title: E9591_15H_Treated, biological repB

description: Gene expression data from yeast cells treated with E9591 for 15h (repB)

source_name: Yeast cells treated with E9591 for 15H


title: BIPRandPHEN_4H_DMSO_Control, biological repB

description: Gene expression data from yeast cells treated with DMSO for 4h (repB)


title: BIPRandPHEN_4H_DMSO_Control, biological repA

description: Gene expression data from yeast cells treated with DMSO for 4h (repA)


title: E9591andLMT_15H_DMSO_Control, biological repA

description: Gene expression data from yeast cells treated with DMSO for 15h (repA)

source_name: Yeast cells treated with DMSO for 15H


title: E9591andLMT_15H_DMSO_Control, biological repB

description: Gene expression data from yeast cells treated with DMSO for 15h (repB)


title: E9591andLMT_15H_DMSO_Control, biological repC

description: Gene expression data from yeast cells treated with DMSO for 15h (repC)


title: E9591_15H_Treated, biological repC

description: Gene expression data from yeast cells treated with E9591 for 15h (repC)


title: LMT_4H_Treated, biological repA

description: Gene expression data from yeast cells treated with LMT for 4h (repA)

source_name: Yeast cells treated with LMT for 4H


title: LMT_15H_Treated, biological repB

description: Gene expression data from yeast cells treated with LMT for 15h (repB)


title: E9591_15H_Treated, biological repA

description: Gene expression data from yeast cells treated with E9591 for 15h (repA)


title: LMT_4H_Treated, biological repC

description: Gene expression data from yeast cells treated with LMT for 4h (repC)


title: LMT_4H_DMSO_Control, biological repC

description: Gene expression data from yeast cells treated with DMSO for 4h (repC)


title: LMT_4H_DMSO_Control, biological repB


title: LMT_4H_DMSO_Control, biological repA


title: LMT_4H_Treated, biological repB

description: Gene expression data from yeast cells treated with LMT for 4h (repB)


title: E9591_4H_Treated, biological repA

description: Gene expression data from yeast cells treated with E9591 for 4h (repA)

source_name: Yeast cells treated with E9591 for 4H


title: E9591_4H_DMSO_Control, biological repA


title: E9591_4H_DMSO_Control, biological repC


title: E9591_4H_Treated, biological repC

description: Gene expression data from yeast cells treated with E9591 for 4h (repC)


title: E9591_4H_DMSO_Control, biological repB


title: E9591_4H_Treated, biological repB

description: Gene expression data from yeast cells treated with E9591 for 4h (repB)


title: C57BL/6 spleen tissues infected by Ectromelia virus at 3 day post infection

city: Lanzhou

first: Wenyu

last: Cheng

state: Gansu

department: State Key Laboratory of Veterinary Etiological Biology

institute: Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences

description: Gene expression data from mouse C57BL6 spleen tissues infected by Ectromelia virus at 3 days

hyb_protocol: Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45℃ 60 rpm on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.

scan_protocol: GeneChips were scanned using the Affymetrix GeneChip® Scanner 3000.

data_processing: The data were analyzed with CapitalBio® Molecule Annotation System V3.0 (MAS3.0) using CapitalBio (Beijing, China) default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.

source_name: spleen tissues of ECTV infected at 3 day post infection

label_protocol: Biotinylated cRNA were prepared according to the standard Affymetrix protocol.

growth_protocol: Seven- to nine-week-old BALB/c and C57BL/6 SPF mice infected with 104 TCID50/mL ECTV (in 100 µL PBS).

extract_protocol: Trizol (Invitrogen, Carlsbad, CA,USA) extraction of total RNA was performed according to the manufacturer's instructions.

treatment_protocol: Spleen tissue from each group were pooled and cut into pieces,A total of 1.6 grams pooled spleen tissues was divided into 4 tubes,and then one tubes tissues were subjrcted to microarray experiment.

value: pooled male and female

value: spleen

tag: infection

value: ECTV

tag: time

value: 3 days post infection


title: LMT_15H_Treated, biological repA

description: Gene expression data from yeast cells treated with LMT for 15h (repA)


title: Balb/c spleen tissues infected by Ectromelia virus at 3 day post infection

description: Gene expression data from mouse Balb/c spleen tissues infected by Ectromelia virus at 3 days

value: BALB/c


title: C57BL/6 spleen tissues infected by Ectromelia virus at 10 day post infection

description: Gene expression data from mouse C57BL6 spleen tissues infected by Ectromelia virus at 10 days

source_name: spleen tissues of ECTV infected at 10 day post infection

value: 10 days post infection


title: C57BL/6 spleen tissues non-infected at 0 hour

description: Gene expression data from mouse C57BL6 spleen tissues

source_name: spleen tissues,non-infected

value: 0 hour


title: Balb/c spleen tissues non-infected at 0 hour

description: Gene expression data from mouse Balb/c spleen tissues


title: RT112 - lipo#3

city: paris

first: Elodie

last: chapeaublanc

address: 26 rue d'ulm

department: UMR 144

country: France

institute: Institut Curie

description: RT112 treated with LipofectaminRNAimax

hyb_protocol: Affymetrix microarrays were hybridized with 10µg of biotinylated cRNA according the supplier recommendations. Microarrays were washed and stained using FS450 fluidics upon a protocol provided by Affymetrix, corresponding to the U133plus 2.0 arrays

scan_protocol: Microarrays were scanned using a 7G, Affymetrix GCS3000 scanner, raw data were acquired with Affymetrix Genechip Command Console.

data_processing: Signal reconstruction was performed with the RMA algorithm as implemented in Bioconductor affy package on R statistical software using the Dai custom CDF package: HGU133Plus2_Hs_ENTREZG Brainarray Custom CDF EntrezG version 18

source_name: human bladder cancer cell line, RT112

label_protocol: 100ng of total RNA were amplified to produced biotinylated complementary RNAs, as recommended by Affymetrix.

growth_protocol: The bladder cancer cells, RT112, have been culture in RPM1 10%fetal bobine serum

extract_protocol: mRNAs were extracted and purified using RNeasy Mini kits (Qiagen).

treatment_protocol: RT112 cells were transfected for 40h either with siRNA FGFR3#3 (ThermoFisher Scientific, s5167) or siRNA FGFR3#4 (ThermoFisher Scientific, s5168) using LipofectaminRNAimax (Invitrogen) according to the manufacturer's instructions (reverse protocol)


title: Balb/c spleen tissues infected by Ectromelia virus at 10 day post infection

description: Gene expression data from mouse Balb/c spleen tissues infected by Ectromelia virus at 10 days


title: RT112 - siFGFR3#3 - 5 nM

description: RT112 treated with siRNA FGFR3#3


title: SpVATCtrl_rep2a2155

city: Boston

first: CBDM

last: Lab

state: MA

address: 77 Avenue Louis Pasteur

department: Microbiology and Immunobiology

institute: Harvard Medical School

data_processing:
fastq quality read filtering (>80% with Sanger Q>20)
cell barcode demultiplexing (custom script)
mapping with tophat2 on the mm10 transcriptome
filtering out low alignment score reads and multiple mapping reads (samtools)
duplication filtering using unique molecular identifiers (custom scripts). UMI sequence is at the 4rth position in the read ID (separation by ":")
Genome_build: mm10
Supplementary_files_format_and_content: raw read counts

source_name: spleen

growth_protocol: Live cells were sorted by flow cytometry from the spleen and visceral adipose tissue of mice (carrying a Foxp3 reporter gene).

extract_protocol:
Spleen were mechanically dissociated and visceral adipose tissue were digested with collagenase/dispase to single cell suspensions. Tregs cells were sorted by flow cytometry as DAPI- TCRb+ CD4+ GFP+ cells. Transgenic cells were sorted as TRAV2+ TRBV4+ cells. Single cells were then encapsulated in nanodroplets according to the InDrops protocol.
mRNA was reverse transcribed using adapters containing oligo dT in each droplet. The emulsion was then broken and linear amplification was performed by in vitro transcription. Illumina adapters were added by reverse transcription and PCR. Library were sequenced using a NextSeq500.

value: spleen and visceral adipose

tag: library name

tag: sample type

value: B6 Foxp3-Ires-Gfp

tag: Sex

value: male

tag: age (months)

value: notTg

tag: total number of reads

tag: number of mapped reads

tag: number of unique reads (umi)


title: SpVATCtrl_rep2a2137

tag: tissue


title: SpVATCtrl_rep2a2152


title: SpVATCtrl_rep2a2154


title: SpVATCtrl_rep2a2139


title: SpVATCtrl_rep2a2153


title: SpVATCtrl_rep2a2147


title: SpVATCtrl_rep2a2145


title: SpVATCtrl_rep2a2136


title: RT112 - siFGFR3#4 - 5 nM

description: RT112 treated with siRNA FGFR3#4


title: SpVATCtrl_rep2a2117


title: RT112 - lipo#1

description: RT112 treated with LipofectaminRNAimax


title: SpVATCtrl_rep2a2110


title: SpVATCtrl_rep2a2113


title: SpVATCtrl_rep2a2127


title: SpVATCtrl_rep2a2125


title: SpVATCtrl_rep2a2115


title: SpVATCtrl_rep2a2108


title: RT112 - lipo#2

description: RT112 treated with LipofectaminRNAimax


title: SpVATCtrl_rep2a2119


title: SpVATCtrl_rep2a2103


title: SpVATCtrl_rep2a2107


title: SpVATCtrl_rep2a2095


title: SpVATCtrl_rep2a2099


title: SpVATCtrl_rep2a2098


title: SpVATCtrl_rep2a2083


title: SpVATCtrl_rep2a2118


title: SpVATCtrl_rep2a2100


title: SpVATCtrl_rep2a2094


title: SpVATCtrl_rep2a2089


title: SpVATCtrl_rep2a2093


title: SpVATCtrl_rep2a2085


title: SpVATCtrl_rep2a2063


title: SpVATCtrl_rep2a2069


title: SpVATCtrl_rep2a2076


title: SpVATCtrl_rep2a2061


title: SpVATCtrl_rep2a2071


title: SpVATCtrl_rep2a2028


title: SpVATCtrl_rep2a2042


title: SpVATCtrl_rep2a2040


title: SpVATCtrl_rep2a2029


title: SpVATCtrl_rep2a2027


title: SpVATCtrl_rep2a2026


title: SpVATCtrl_rep2a2037


title: SpVATCtrl_rep2a2023


title: SpVATCtrl_rep2a2020


title: SpVATCtrl_rep2a2051


title: SpVATCtrl_rep2a2013


title: SpVATCtrl_rep2a2022


title: SpVATCtrl_rep2a2012


title: SpVATCtrl_rep2a2011


title: SpVATCtrl_rep2a2003


title: SpVATCtrl_rep2a2004


title: SpVATCtrl_rep2a2000


title: SpVATCtrl_rep2a1997


title: SpVATCtrl_rep2a2044


title: SpVATCtrl_rep2a1987


title: SpVATCtrl_rep2a1988


title: SpVATCtrl_rep2a1991


title: SpVATCtrl_rep2a1994


title: SpVATCtrl_rep2a2018


title: SpVATCtrl_rep2a1986


title: SpVATCtrl_rep2a1984


title: SpVATCtrl_rep2a1975


title: SpVATCtrl_rep2a1974


title: SpVATCtrl_rep2a1971


title: SpVATCtrl_rep2a1963


title: SpVATCtrl_rep2a1967


title: SpVATCtrl_rep2a1961


title: SpVATCtrl_rep2a1948


title: SpVATCtrl_rep2a1951


title: SpVATCtrl_rep2a1956


title: SpVATCtrl_rep2a1958


title: SpVATCtrl_rep2a1993


title: SpVATCtrl_rep2a1941


title: SpVATCtrl_rep2a1943


title: SpVATCtrl_rep2a1944


title: SpVATCtrl_rep2a1938


title: SpVATCtrl_rep2a1937


title: SpVATCtrl_rep2a1936


title: SpVATCtrl_rep2a1934


title: SpVATCtrl_rep2a1929


title: SpVATCtrl_rep2a1978


title: SpVATCtrl_rep2a1977


title: SpVATCtrl_rep2a1980


title: SpVATCtrl_rep2a1982


title: SpVATCtrl_rep2a1973


title: SpVATCtrl_rep2a1923


title: SpVATCtrl_rep2a1922


title: SpVATCtrl_rep2a1921


title: SpVATCtrl_rep2a1946


title: SpVATCtrl_rep2a1919


title: SpVATCtrl_rep2a1918


title: SpVATCtrl_rep2a1914


title: SpVATCtrl_rep2a1920


title: SpVATCtrl_rep2a1904


title: SpVATCtrl_rep2a1907


title: SpVATCtrl_rep2a1892


title: SpVATCtrl_rep2a1898


title: SpVATCtrl_rep2a1879


title: SpVATCtrl_rep2a1874


title: SpVATCtrl_rep2a1870


title: SpVATCtrl_rep2a1886


title: SpVATCtrl_rep2a1928


title: SpVATCtrl_rep2a1867


title: SpVATCtrl_rep2a1895


title: SpVATCtrl_rep2a1931


title: SpVATCtrl_rep2a1864


title: SpVATCtrl_rep2a1861


title: SpVATCtrl_rep2a1869


title: SpVATCtrl_rep2a1858


title: SpVATCtrl_rep2a1857


title: SpVATCtrl_rep2a1855


title: SpVATCtrl_rep2a1856


title: SpVATCtrl_rep2a1865


title: SpVATCtrl_rep2a1854


title: SpVATCtrl_rep2a1853


title: SpVATCtrl_rep2a1850


title: SpVATCtrl_rep2a1875


title: SpVATCtrl_rep2a1844


title: SpVATCtrl_rep2a1846


title: SpVATCtrl_rep2a1845


title: SpVATCtrl_rep2a1847


title: SpVATCtrl_rep2a1836


title: SpVATCtrl_rep2a1839


title: SpVATCtrl_rep2a1840


title: SpVATCtrl_rep2a1828


title: SpVATCtrl_rep2a1834


title: SpVATCtrl_rep2a1826


title: SpVATCtrl_rep2a1807


title: SpVATCtrl_rep2a1817


title: SpVATCtrl_rep2a1830


title: SpVATCtrl_rep2a1811


title: SpVATCtrl_rep2a1849


title: SpVATCtrl_rep2a1822


title: SpVATCtrl_rep2a1797


title: SpVATCtrl_rep2a1799


title: SpVATCtrl_rep2a1798


title: SpVATCtrl_rep2a1813


title: SpVATCtrl_rep2a1806


title: SpVATCtrl_rep2a1796


title: SpVATCtrl_rep2a1803


title: SpVATCtrl_rep2a1793


title: SpVATCtrl_rep2a1794


title: SpVATCtrl_rep2a1792


title: SpVATCtrl_rep2a1789


title: SpVATCtrl_rep2a1786


title: SpVATCtrl_rep2a1841


title: SpVATCtrl_rep2a1777


title: SpVATCtrl_rep2a1776


title: SpVATCtrl_rep2a1767


title: SpVATCtrl_rep2a1769


title: SpVATCtrl_rep2a1827


title: SpVATCtrl_rep2a1763


title: SpVATCtrl_rep2a1764


title: SpVATCtrl_rep2a1754


title: SpVATCtrl_rep2a1755


title: SpVATCtrl_rep2a1760


title: SpVATCtrl_rep2a1750


title: SpVATCtrl_rep2a1744


title: SpVATCtrl_rep2a1751


title: SpVATCtrl_rep2a1762


title: SpVATCtrl_rep2a1785


title: SpVATCtrl_rep2a1733


title: SpVATCtrl_rep2a1735


title: SpVATCtrl_rep2a1740


title: SpVATCtrl_rep2a1742


title: SpVATCtrl_rep2a1739


title: SpVATCtrl_rep2a1736


title: SpVATCtrl_rep2a1731


title: SpVATCtrl_rep2a1737


title: SpVATCtrl_rep2a1718


title: SpVATCtrl_rep2a1730


title: SpVATCtrl_rep2a1713


title: SpVATCtrl_rep2a1714


title: SpVATCtrl_rep2a1719


title: SpVATCtrl_rep2a1723


title: SpVATCtrl_rep2a1766


title: SpVATCtrl_rep2a1711


title: SpVATCtrl_rep2a1708


title: SpVATCtrl_rep2a1707


title: SpVATCtrl_rep2a1706


title: SpVATCtrl_rep2a1705


title: SpVATCtrl_rep2a1704


title: SpVATCtrl_rep2a1697


title: SpVATCtrl_rep2a1703


title: SpVATCtrl_rep2a1695


title: SpVATCtrl_rep2a1685


title: SpVATCtrl_rep2a1692


title: SpVATCtrl_rep2a1673


title: SpVATCtrl_rep2a1686


title: SpVATCtrl_rep2a1675


title: SpVATCtrl_rep2a1671


title: SpVATCtrl_rep2a1694


title: SpVATCtrl_rep2a1676


title: SpVATCtrl_rep2a1667


title: SpVATCtrl_rep2a1669


title: SpVATCtrl_rep2a1666


title: SpVATCtrl_rep2a1668


title: SpVATCtrl_rep2a1664


title: SpVATCtrl_rep2a1660


title: SpVATCtrl_rep2a1656


title: SpVATCtrl_rep2a1658


title: SpVATCtrl_rep2a1645


title: SpVATCtrl_rep2a1701


title: SpVATCtrl_rep2a1702


title: SpVATCtrl_rep2a1654


title: SpVATCtrl_rep2a1648


title: SpVATCtrl_rep2a1652


title: SpVATCtrl_rep2a1647


title: SpVATCtrl_rep2a1643


title: SpVATCtrl_rep2a1639


title: SpVATCtrl_rep2a1631


title: SpVATCtrl_rep2a1712


title: SpVATCtrl_rep2a1637


title: SpVATCtrl_rep2a1630


title: SpVATCtrl_rep2a1620


title: SpVATCtrl_rep2a1624


title: SpVATCtrl_rep2a1634


title: SpVATCtrl_rep2a1629


title: SpVATCtrl_rep2a1619


title: SpVATCtrl_rep2a1614


title: SpVATCtrl_rep2a1621


title: SpVATCtrl_rep2a1626


title: SpVATCtrl_rep2a1613


title: SpVATCtrl_rep2a1610


title: SpVATCtrl_rep2a1618


title: SpVATCtrl_rep2a1601


title: SpVATCtrl_rep2a1608


title: SpVATCtrl_rep2a1598


title: SpVATCtrl_rep2a1597


title: SpVATCtrl_rep2a1596


title: SpVATCtrl_rep2a1595


title: SpVATCtrl_rep2a1586


title: SpVATCtrl_rep2a1594


title: SpVATCtrl_rep2a1592


title: SpVATCtrl_rep2a1582


title: SpVATCtrl_rep2a1577


title: SpVATCtrl_rep2a1574


title: SpVATCtrl_rep2a1575


title: SpVATCtrl_rep2a1585


title: SpVATCtrl_rep2a1589


title: SpVATCtrl_rep2a1579


title: SpVATCtrl_rep2a1561


title: SpVATCtrl_rep2a1560


title: SpVATCtrl_rep2a1546


title: SpVATCtrl_rep2a1550


title: SpVATCtrl_rep2a1552


title: SpVATCtrl_rep2a1557


title: SpVATCtrl_rep2a1532


title: SpVATCtrl_rep2a1519


title: SpVATCtrl_rep2a1518


title: SpVATCtrl_rep2a1525


title: SpVATCtrl_rep2a1505


title: SpVATCtrl_rep2a1497


title: SpVATCtrl_rep2a1568


title: SpVATCtrl_rep2a1498


title: SpVATCtrl_rep2a1493


title: SpVATCtrl_rep2a1494


title: SpVATCtrl_rep2a1496


title: SpVATCtrl_rep2a1495


title: SpVATCtrl_rep2a1492


title: SpVATCtrl_rep2a1489


title: SpVATCtrl_rep2a1564


title: SpVATCtrl_rep2a1491


title: SpVATCtrl_rep2a1474


title: SpVATCtrl_rep2a1478


title: SpVATCtrl_rep2a1542


title: SpVATCtrl_rep2a1528


title: SpVATCtrl_rep2a1471


title: SpVATCtrl_rep2a1470


title: SpVATCtrl_rep2a1464


title: SpVATCtrl_rep2a1460


title: SpVATCtrl_rep2a1469


title: SpVATCtrl_rep2a1517


title: SpVATCtrl_rep2a1462


title: SpVATCtrl_rep2a1513


title: SpVATCtrl_rep2a1458


title: SpVATCtrl_rep2a1456


title: SpVATCtrl_rep2a1453


title: SpVATCtrl_rep2a1452


title: SpVATCtrl_rep2a1454


title: SpVATCtrl_rep2a1455


title: SpVATCtrl_rep2a1479


title: SpVATCtrl_rep2a1481


title: SpVATCtrl_rep2a1450


title: SpVATCtrl_rep2a1451


title: SpVATCtrl_rep2a1446


title: SpVATCtrl_rep2a1449


title: SpVATCtrl_rep2a1448


title: SpVATCtrl_rep2a1438


title: SpVATCtrl_rep2a1440


title: SpVATCtrl_rep2a1439


title: SpVATCtrl_rep2a1436


title: SpVATCtrl_rep2a1431


title: SpVATCtrl_rep2a1435


title: SpVATCtrl_rep2a1432


title: SpVATCtrl_rep2a1426


title: SpVATCtrl_rep2a1423


title: SpVATCtrl_rep2a1425


title: SpVATCtrl_rep2a1424


title: SpVATCtrl_rep2a1421


title: SpVATCtrl_rep2a1418


title: SpVATCtrl_rep2a1414


title: SpVATCtrl_rep2a1420


title: SpVATCtrl_rep2a1413


title: SpVATCtrl_rep2a1407


title: SpVATCtrl_rep2a1406


title: SpVATCtrl_rep2a1410


title: SpVATCtrl_rep2a1401


title: SpVATCtrl_rep2a1400


title: SpVATCtrl_rep2a1396


title: SpVATCtrl_rep2a1402


title: SpVATCtrl_rep2a1403


title: SpVATCtrl_rep2a1408


title: SpVATCtrl_rep2a1385


title: SpVATCtrl_rep2a1394


title: SpVATCtrl_rep2a1390


title: SpVATCtrl_rep2a1433


title: SpVATCtrl_rep2a1383


title: SpVATCtrl_rep2a1397


title: SpVATCtrl_rep2a1384


title: SpVATCtrl_rep2a1380


title: SpVATCtrl_rep2a1382


title: SpVATCtrl_rep2a1371


title: SpVATCtrl_rep2a1366


title: SpVATCtrl_rep2a1369


title: SpVATCtrl_rep2a1352


title: SpVATCtrl_rep2a1360


title: SpVATCtrl_rep2a1347


title: SpVATCtrl_rep2a1345


title: SpVATCtrl_rep2a1387


title: SpVATCtrl_rep2a1344


title: SpVATCtrl_rep2a1339


title: SpVATCtrl_rep2a1332


title: SpVATCtrl_rep2a1324


title: SpVATCtrl_rep2a1333


title: SpVATCtrl_rep2a1340


title: SpVATCtrl_rep2a1336


title: SpVATCtrl_rep2a1328


title: SpVATCtrl_rep2a1335


title: SpVATCtrl_rep2a1326


title: SpVATCtrl_rep2a1320


title: SpVATCtrl_rep2a1311


title: SpVATCtrl_rep2a1316


title: SpVATCtrl_rep2a1317


title: SpVATCtrl_rep2a1308


title: SpVATCtrl_rep2a1310


title: SpVATCtrl_rep2a1321


title: SpVATCtrl_rep2a1307


title: SpVATCtrl_rep2a1409


title: SpVATCtrl_rep2a1372


title: SpVATCtrl_rep2a1304


title: SpVATCtrl_rep2a1301


title: SpVATCtrl_rep2a1298


title: SpVATCtrl_rep2a1348


title: SpVATCtrl_rep2a1297


title: SpVATCtrl_rep2a1305


title: SpVATCtrl_rep2a1296


title: SpVATCtrl_rep2a1292


title: SpVATCtrl_rep2a1290


title: SpVATCtrl_rep2a1277


title: SpVATCtrl_rep2a1284


title: SpVATCtrl_rep2a1275


title: SpVATCtrl_rep2a1285


title: SpVATCtrl_rep2a1272


title: SpVATCtrl_rep2a1268


title: SpVATCtrl_rep2a1271


title: SpVATCtrl_rep2a1261


title: SpVATCtrl_rep2a1260


title: SpVATCtrl_rep2a1253


title: SpVATCtrl_rep2a1241


title: SpVATCtrl_rep2a1242


title: SpVATCtrl_rep2a1258


title: SpVATCtrl_rep2a1243


title: SpVATCtrl_rep2a1239


title: SpVATCtrl_rep2a1235


title: SpVATCtrl_rep2a1232


title: SpVATCtrl_rep2a1218


title: SpVATCtrl_rep2a1222


title: SpVATCtrl_rep2a1220


title: SpVATCtrl_rep2a1216


title: SpVATCtrl_rep2a1217


title: SpVATCtrl_rep2a1215


title: SpVATCtrl_rep2a1213


title: SpVATCtrl_rep2a1287


title: SpVATCtrl_rep2a1211


title: SpVATCtrl_rep2a1281


title: SpVATCtrl_rep2a1209


title: SpVATCtrl_rep2a1210


title: SpVATCtrl_rep2a1202


title: SpVATCtrl_rep2a1203


title: SpVATCtrl_rep2a1204


title: SpVATCtrl_rep2a1219


title: SpVATCtrl_rep2a1189


title: SpVATCtrl_rep2a1185


title: SpVATCtrl_rep2a1183


title: SpVATCtrl_rep2a1186


title: SpVATCtrl_rep2a1191


title: SpVATCtrl_rep2a1177


title: SpVATCtrl_rep2a1178


title: SpVATCtrl_rep2a1214


title: SpVATCtrl_rep2a1173


title: SpVATCtrl_rep2a1165


title: SpVATCtrl_rep2a1161


title: SpVATCtrl_rep2a1158


title: SpVATCtrl_rep2a1361


title: SpVATCtrl_rep2a1167


title: SpVATCtrl_rep2a1153


title: SpVATCtrl_rep2a1156


title: SpVATCtrl_rep2a1155


title: SpVATCtrl_rep2a1208


title: SpVATCtrl_rep2a1152


title: SpVATCtrl_rep2a1147


title: SpVATCtrl_rep2a1198


title: SpVATCtrl_rep2a1146


title: SpVATCtrl_rep2a1140


title: SpVATCtrl_rep2a1132


title: SpVATCtrl_rep2a1196


title: SpVATCtrl_rep2a1134


title: SpVATCtrl_rep2a1192


title: SpVATCtrl_rep2a1129


title: SpVATCtrl_rep2a1123


title: SpVATCtrl_rep2a1116


title: SpVATCtrl_rep2a1115


title: SpVATCtrl_rep2a1114


title: SpVATCtrl_rep2a1121


title: SpVATCtrl_rep2a1159


title: SpVATCtrl_rep2a1113


title: SpVATCtrl_rep2a1127


title: SpVATCtrl_rep2a1101


title: SpVATCtrl_rep2a1100


title: SpVATCtrl_rep2a1092


title: SpVATCtrl_rep2a1090


title: SpVATCtrl_rep2a1093


title: SpVATCtrl_rep2a1087


title: SpVATCtrl_rep2a1086


title: SpVATCtrl_rep2a1144


title: SpVATCtrl_rep2a1118


title: SpVATCtrl_rep2a1084


title: SpVATCtrl_rep2a1085


title: SpVATCtrl_rep2a1080


title: SpVATCtrl_rep2a1077


title: SpVATCtrl_rep2a1079


title: SpVATCtrl_rep2a1072


title: SpVATCtrl_rep2a1071


title: SpVATCtrl_rep2a1069


title: SpVATCtrl_rep2a1068


title: SpVATCtrl_rep2a1097


title: SpVATCtrl_rep2a1096


title: SpVATCtrl_rep2a1057


title: SpVATCtrl_rep2a1056


title: SpVATCtrl_rep2a1067


title: SpVATCtrl_rep2a1055


title: SpVATCtrl_rep2a1052


title: SpVATCtrl_rep2a1053


title: SpVATCtrl_rep2a1034


title: SpVATCtrl_rep2a1046


title: SpVATCtrl_rep2a1044


title: SpVATCtrl_rep2a1035


title: SpVATCtrl_rep2a1042


title: SpVATCtrl_rep2a1033


title: SpVATCtrl_rep2a1030


title: SpVATCtrl_rep2a1032


title: SpVATCtrl_rep2a1019


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title: SpVATCtrl_rep2_519

growth_protocol: Live cells were sorted by flow cytometry from the spleen and visceral adipose tissue of mice.

extract_protocol:
Spleen were mechanically dissociated and visceral adipose tissue were digested with collagenase/dispase to single cell suspensions. Tregs were sorted by flow cytometry as DAPI- TCRb+ CD4+ CD25+ cells. Single cells were then encapsulated in nanodroplets according to the InDrops protocol.
mRNA was reverse transcribed using adapters containing oligo dT in each droplet. The emulsion was then broken and linear amplification was performed by in vitro transcription. Illumina adapters were added by reverse transcription and PCR. Library were sequenced using a NextSeq500.


title: SpVATCtrl_rep2a56


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title: SpVATCtrl_rep2_296


title: SpVATCtrl_rep2_304


title: SpVATCtrl_rep2_325


title: SpVATCtrl_rep2_294


title: SpVATCtrl_rep2_311


title: SpVATCtrl_rep2_317


title: SpVATCtrl_rep2_291


title: SpVATCtrl_rep2_320


title: SpVATCtrl_rep2_341


title: SpVATCtrl_rep2_290


title: SpVATCtrl_rep2_330


title: SpVATCtrl_rep2_289


title: SpVATCtrl_rep2_288


title: SpVATCtrl_rep2_284


title: SpVATCtrl_rep2_313


title: SpVATCtrl_rep2_315


title: SpVATCtrl_rep2_323


title: SpVATCtrl_rep2_283


title: SpVATCtrl_rep2_285


title: SpVATCtrl_rep2_300


title: SpVATCtrl_rep2_292


title: SpVATCtrl_rep2_340


title: SpVATCtrl_rep2_287


title: SpVATCtrl_rep2_281


title: SpVATCtrl_rep2_279


title: SpVATCtrl_rep2_282


title: SpVATCtrl_rep2_280


title: SpVATCtrl_rep2_276


title: SpVATCtrl_rep2_278


title: SpVATCtrl_rep2_272


title: SpVATCtrl_rep2_274


title: SpVATCtrl_rep2_277


title: SpVATCtrl_rep2_269


title: SpVATCtrl_rep2_263


title: SpVATCtrl_rep2_265


title: SpVATCtrl_rep2_259


title: SpVATCtrl_rep2_266


title: SpVATCtrl_rep2_256


title: SpVATCtrl_rep2_260


title: SpVATCtrl_rep2_249


title: SpVATCtrl_rep2_253


title: SpVATCtrl_rep2_248


title: SpVATCtrl_rep2_243


title: SpVATCtrl_rep2_240


title: SpVATCtrl_rep2_245


title: SpVATCtrl_rep2_241


title: SpVATCtrl_rep2_244


title: SpVATCtrl_rep2_237


title: SpVATCtrl_rep2_233


title: SpVATCtrl_rep2_235


title: SpVATCtrl_rep2_228


title: SpVATCtrl_rep2_231


title: SpVATCtrl_rep2_268


title: SpVATCtrl_rep2_230


title: SpVATCtrl_rep2_232


title: SpVATCtrl_rep2_225


title: SpVATCtrl_rep2_227


title: SpVATCtrl_rep2_224


title: SpVATCtrl_rep2_254


title: SpVATCtrl_rep2_222


title: SpVATCtrl_rep2_223


title: SpVATCtrl_rep2_219


title: SpVATCtrl_rep2_218


title: SpVATCtrl_rep2_217


title: SpVATCtrl_rep2_220


title: SpVATCtrl_rep2_262


title: SpVATCtrl_rep2_213


title: SpVATCtrl_rep2_238


title: SpVATCtrl_rep2_215


title: SpVATCtrl_rep2_229


title: SpVATCtrl_rep2_216


title: SpVATCtrl_rep2_208


title: SpVATCtrl_rep2_210


title: SpVATCtrl_rep2_214


title: SpVATCtrl_rep2_206


title: SpVATCtrl_rep2_205


title: SpVATCtrl_rep2_202


title: SpVATCtrl_rep2_201


title: SpVATCtrl_rep2_204


title: SpVATCtrl_rep2_200


title: SpVATCtrl_rep2_196


title: SpVATCtrl_rep2_193


title: SpVATCtrl_rep2_195


title: SpVATCtrl_rep2_187


title: SpVATCtrl_rep2_188


title: SpVATCtrl_rep2_194


title: SpVATCtrl_rep2_184


title: SpVATCtrl_rep2_183


title: SpVATCtrl_rep2_192


title: SpVATCtrl_rep2_185


title: SpVATCtrl_rep2_191


title: SpVATCtrl_rep2_182


title: SpVATCtrl_rep2_180


title: SpVATCtrl_rep2_179


title: SpVATCtrl_rep2_177


title: SpVATCtrl_rep2_212


title: SpVATCtrl_rep2_176


title: SpVATCtrl_rep2_178


title: SpVATCtrl_rep2_169


title: SpVATCtrl_rep2_168


title: SpVATCtrl_rep2_174


title: SpVATCtrl_rep2_166


title: SpVATCtrl_rep2_173


title: SpVATCtrl_rep2_162


title: SpVATCtrl_rep2_167


title: SpVATCtrl_rep2_165


title: SpVATCtrl_rep2_171


title: SpVATCtrl_rep2_161


title: SpVATCtrl_rep2_164


title: SpVATCtrl_rep2_157


title: SpVATCtrl_rep2_163


title: SpVATCtrl_rep2_155


title: SpVATCtrl_rep2_151


title: SpVATCtrl_rep2_159


title: SpVATCtrl_rep2_150


title: SpVATCtrl_rep2_146


title: SpVATCtrl_rep2_140


title: SpVATCtrl_rep2_156


title: SpVATCtrl_rep2_139


title: SpVATCtrl_rep2_145


title: SpVATCtrl_rep2_137


title: SpVATCtrl_rep2_138


title: SpVATCtrl_rep2_170


title: SpVATCtrl_rep2_130


title: SpVATCtrl_rep2_127


title: SpVATCtrl_rep2_135


title: SpVATCtrl_rep2_133


title: SpVATCtrl_rep2_132


title: SpVATCtrl_rep2_134


title: SpVATCtrl_rep2_120


title: SpVATCtrl_rep2_153


title: SpVATCtrl_rep2_119


title: SpVATCtrl_rep2_123


title: SpVATCtrl_rep2_122


title: SpVATCtrl_rep2_117


title: SpVATCtrl_rep2_114


title: SpVATCtrl_rep2_126


title: SpVATCtrl_rep2_118


title: SpVATCtrl_rep2_121


title: SpVATCtrl_rep2_116


title: SpVATCtrl_rep2_115


title: SpVATCtrl_rep2_109


title: SpVATCtrl_rep2_108


title: SpVATCtrl_rep2_107


title: SpVATCtrl_rep2_141


title: SpVATCtrl_rep2_113


title: SpVATCtrl_rep2_101


title: SpVATCtrl_rep2_106


title: SpVATCtrl_rep2_97


title: SpVATCtrl_rep2_95


title: SpVATCtrl_rep2_93


title: SpVATCtrl_rep2_105


title: SpVATCtrl_rep2_102


title: SpVATCtrl_rep2_87


title: SpVATCtrl_rep2_86


title: SpVATCtrl_rep2_78


title: SpVATCtrl_rep2_90


title: SpVATCtrl_rep2_82


title: SpVATCtrl_rep2_80


title: SpVATCtrl_rep2_110


title: SpVATCtrl_rep2_92


title: SpVATCtrl_rep2_88


title: SpVATCtrl_rep2_84


title: SpVATCtrl_rep2_74


title: SpVATCtrl_rep2_96


title: SpVATCtrl_rep2_85


title: SpVATCtrl_rep2_81


title: SpVATCtrl_rep2_67


title: SpVATCtrl_rep2_77


title: SpVATCtrl_rep2_103


title: SpVATCtrl_rep2_76


title: SpVATCtrl_rep2_63


title: SpVATCtrl_rep2_61


title: SpVATCtrl_rep2_75


title: SpVATCtrl_rep2_70


title: SpVATCtrl_rep2_60


title: SpVATCtrl_rep2_69


title: SpVATCtrl_rep2_59


title: SpVATCtrl_rep2_91


title: SpVATCtrl_rep2_54


title: SpVATCtrl_rep2_56


title: SpVATCtrl_rep2_71


title: SpVATCtrl_rep2_47


title: SpVATCtrl_rep2_45


title: SpVATCtrl_rep2_65


title: SpVATCtrl_rep2_64


title: SpVATCtrl_rep2_62


title: SpVATCtrl_rep2_66


title: SpVATCtrl_rep2_83


title: SpVATCtrl_rep2_52


title: SpVATCtrl_rep2_36


title: SpVATCtrl_rep2_48


title: SpVATCtrl_rep2_44


title: SpVATCtrl_rep2_57


title: SpVATCtrl_rep2_33


title: SpVATCtrl_rep2_53


title: SpVATCtrl_rep2_38


title: SpVATCtrl_rep2_32


title: SpVATCtrl_rep2_39


title: SpVATCtrl_rep2_29


title: SpVATCtrl_rep2_35


title: SpVATCtrl_rep2_27


title: SpVATCtrl_rep2_43


title: SpVATCtrl_rep2_30


title: SpVATCtrl_rep2_25


title: SpVATCtrl_rep2_24


title: SpVATCtrl_rep2_42


title: SpVATCtrl_rep2_22


title: SpVATCtrl_rep2_46


title: SpVATCtrl_rep2_18


title: SpVATCtrl_rep2_12


title: SpVATCtrl_rep2_17


title: SpVATCtrl_rep2_15


title: SpVATCtrl_rep2_11


title: SpVATCtrl_rep2_14


title: SpVATCtrl_rep2_16


title: SpVATCtrl_rep2_9


title: SpVATCtrl_rep2_10


title: SpVATCtrl_rep2_8


title: SpVATCtrl_rep2_6


title: SpVATCtrl_rep2_21


title: SpVATCtrl_rep2_1


title: SpVATCtrl_rep2_5


title: SpVATCtrl_rep2_4


title: SpVATCtrl_rep2_2


title: SpleenTRNurr77Low_rep1_2362

growth_protocol: Live cells were sorted by flow cytometry from the spleen of mice (carrying Foxp3 and Nr4a1 reporter genes).

extract_protocol:
Spleen were mechanically dissociated to single cell suspensions. Tregs were sorted by flow cytometry as DAPI- TCRb+ CD4+ FoxP3-Thy1.1+ cells in 3 different bins of Nr4a1-GFP expression (low=0-20%, med=40-60%, high=80-100%). Single cells were then encapsulated in nanodroplets according to the InDrops protocol.
mRNA was reverse transcribed using adapters containing oligo dT in each droplet. The emulsion was then broken and linear amplification was performed by in vitro transcription. Illumina adapters were added by reverse transcription and PCR. Library were sequenced using a NextSeq500.

value: B6 Nr4a1-Gfp Foxp3-Ires-Thy1.1


title: SpleenTRNurr77Low_rep1_2367


title: SpleenTRNurr77Low_rep1_2370


title: SpleenTRNurr77Low_rep1_2366


title: SpleenTRNurr77Low_rep1_2365


title: SpleenTRNurr77Low_rep1_2363


title: SpleenTRNurr77Low_rep1_2364


title: SpleenTRNurr77Low_rep1_2371


title: SpleenTRNurr77Low_rep1_2368


title: SpleenTRNurr77Low_rep1_2361


title: SpleenTRNurr77Low_rep1_2369


title: SpleenTRNurr77Low_rep1_2360


title: SpleenTRNurr77Low_rep1_2357


title: SpleenTRNurr77Low_rep1_2372


title: SpleenTRNurr77Low_rep1_2358


title: SpleenTRNurr77Low_rep1_2356


title: SpleenTRNurr77Low_rep1_2359


title: SpleenTRNurr77Low_rep1_2352


title: SpleenTRNurr77Low_rep1_2354


title: SpleenTRNurr77Low_rep1_2351


title: SpleenTRNurr77Low_rep1_2355


title: SpleenTRNurr77Low_rep1_2345


title: SpleenTRNurr77Low_rep1_2350


title: SpleenTRNurr77Low_rep1_2349


title: SpleenTRNurr77Low_rep1_2353


title: SpleenTRNurr77Low_rep1_2348


title: SpleenTRNurr77Low_rep1_2347


title: SpleenTRNurr77Low_rep1_2344


title: SpleenTRNurr77Low_rep1_2346


title: SpleenTRNurr77Low_rep1_2343


title: SpleenTRNurr77Low_rep1_2333


title: SpleenTRNurr77Low_rep1_2339


title: SpleenTRNurr77Low_rep1_2338


title: SpleenTRNurr77Low_rep1_2332


title: SpleenTRNurr77Low_rep1_2340


title: SpleenTRNurr77Low_rep1_2342


title: SpleenTRNurr77Low_rep1_2341


title: SpleenTRNurr77Low_rep1_2335


title: SpleenTRNurr77Low_rep1_2329


title: SpleenTRNurr77Low_rep1_2334


title: SpleenTRNurr77Low_rep1_2331


title: SpleenTRNurr77Low_rep1_2324


title: SpleenTRNurr77Low_rep1_2327


title: SpleenTRNurr77Low_rep1_2323


title: SpleenTRNurr77Low_rep1_2319


title: SpleenTRNurr77Low_rep1_2321


title: SpleenTRNurr77Low_rep1_2317


title: SpleenTRNurr77Low_rep1_2328


title: SpleenTRNurr77Low_rep1_2326


title: SpleenTRNurr77Low_rep1_2320


title: SpleenTRNurr77Low_rep1_2315


title: SpleenTRNurr77Low_rep1_2313


title: SpleenTRNurr77Low_rep1_2336


title: SpleenTRNurr77Low_rep1_2337


title: SpleenTRNurr77Low_rep1_2316


title: SpleenTRNurr77Low_rep1_2318


title: SpleenTRNurr77Low_rep1_2314


title: SpleenTRNurr77Low_rep1_2330


title: SpleenTRNurr77Low_rep1_2309


title: SpleenTRNurr77Low_rep1_2312


title: SpleenTRNurr77Low_rep1_2308


title: SpleenTRNurr77Low_rep1_2311


title: SpleenTRNurr77Low_rep1_2325


title: SpleenTRNurr77Low_rep1_2306


title: SpleenTRNurr77Low_rep1_2307


title: SpleenTRNurr77Low_rep1_2305


title: SpleenTRNurr77Low_rep1_2310


title: SpleenTRNurr77Low_rep1_2304


title: SpleenTRNurr77Low_rep1_2302


title: SpleenTRNurr77Low_rep1_2303


title: SpleenTRNurr77Low_rep1_2301


title: SpleenTRNurr77Low_rep1_2322


title: SpleenTRNurr77Low_rep1_2299


title: SpleenTRNurr77Low_rep1_2297


title: SpleenTRNurr77Low_rep1_2296


title: SpleenTRNurr77Low_rep1_2295


title: SpleenTRNurr77Low_rep1_2298


title: SpleenTRNurr77Low_rep1_2292


title: SpleenTRNurr77Low_rep1_2291


title: SpleenTRNurr77Low_rep1_2294


title: SpleenTRNurr77Low_rep1_2293


title: SpleenTRNurr77Low_rep1_2289


title: SpleenTRNurr77Low_rep1_2288


title: SpleenTRNurr77Low_rep1_2286


title: SpleenTRNurr77Low_rep1_2290


title: SpleenTRNurr77Low_rep1_2287


title: SpleenTRNurr77Low_rep1_2285


title: SpleenTRNurr77Low_rep1_2283


title: SpleenTRNurr77Low_rep1_2279


title: SpleenTRNurr77Low_rep1_2281


title: SpleenTRNurr77Low_rep1_2300


title: SpleenTRNurr77Low_rep1_2277


title: SpleenTRNurr77Low_rep1_2274


title: SpleenTRNurr77Low_rep1_2280


title: SpleenTRNurr77Low_rep1_2273


title: SpleenTRNurr77Low_rep1_2275


title: SpleenTRNurr77Low_rep1_2276


title: SpleenTRNurr77Low_rep1_2271


title: SpleenTRNurr77Low_rep1_2268


title: SpleenTRNurr77Low_rep1_2267


title: SpleenTRNurr77Low_rep1_2270


title: SpleenTRNurr77Low_rep1_2265


title: SpleenTRNurr77Low_rep1_2264


title: SpleenTRNurr77Low_rep1_2266


title: SpleenTRNurr77Low_rep1_2269


title: SpleenTRNurr77Low_rep1_2282


title: SpleenTRNurr77Low_rep1_2260


title: SpleenTRNurr77Low_rep1_2258


title: SpleenTRNurr77Low_rep1_2263


title: SpleenTRNurr77Low_rep1_2262


title: SpleenTRNurr77Low_rep1_2259


title: SpleenTRNurr77Low_rep1_2284


title: SpleenTRNurr77Low_rep1_2257


title: SpleenTRNurr77Low_rep1_2255


title: SpleenTRNurr77Low_rep1_2261


title: SpleenTRNurr77Low_rep1_2272


title: SpleenTRNurr77Low_rep1_2256


title: SpleenTRNurr77Low_rep1_2278


title: SpleenTRNurr77Low_rep1_2253


title: SpleenTRNurr77Low_rep1_2250


title: SpleenTRNurr77Low_rep1_2251


title: SpleenTRNurr77Low_rep1_2252


title: SpleenTRNurr77Low_rep1_2248


title: SpleenTRNurr77Low_rep1_2246


title: SpleenTRNurr77Low_rep1_2247


title: SpleenTRNurr77Low_rep1_2249


title: SpleenTRNurr77Low_rep1_2245


title: SpleenTRNurr77Low_rep1_2244


title: SpleenTRNurr77Low_rep1_2242


title: SpleenTRNurr77Low_rep1_2241


title: SpleenTRNurr77Low_rep1_2240


title: SpleenTRNurr77Low_rep1_2239


title: SpleenTRNurr77Low_rep1_2243


title: SpleenTRNurr77Low_rep1_2238


title: SpleenTRNurr77Low_rep1_2254


title: SpleenTRNurr77Low_rep1_2236


title: SpleenTRNurr77Low_rep1_2232


title: SpleenTRNurr77Low_rep1_2237


title: SpleenTRNurr77Low_rep1_2235


title: SpleenTRNurr77Low_rep1_2231


title: SpleenTRNurr77Low_rep1_2234


title: SpleenTRNurr77Low_rep1_2233


title: SpleenTRNurr77Low_rep1_2230


title: SpleenTRNurr77Low_rep1_2228


title: SpleenTRNurr77Low_rep1_2229


title: SpleenTRNurr77Low_rep1_2226


title: SpleenTRNurr77Low_rep1_2224


title: SpleenTRNurr77Low_rep1_2223


title: SpleenTRNurr77Low_rep1_2225


title: SpleenTRNurr77Low_rep1_2227


title: SpleenTRNurr77Low_rep1_2220


title: SpleenTRNurr77Low_rep1_2218


title: SpleenTRNurr77Low_rep1_2221


title: SpleenTRNurr77Low_rep1_2217


title: SpleenTRNurr77Low_rep1_2216


title: SpleenTRNurr77Low_rep1_2219


title: SpleenTRNurr77Low_rep1_2215


title: SpleenTRNurr77Low_rep1_2214


title: SpleenTRNurr77Low_rep1_2212


title: SpleenTRNurr77Low_rep1_2213


title: SpleenTRNurr77Low_rep1_2211


title: SpleenTRNurr77Low_rep1_2207


title: SpleenTRNurr77Low_rep1_2203


title: SpleenTRNurr77Low_rep1_2204


title: SpleenTRNurr77Low_rep1_2208


title: SpleenTRNurr77Low_rep1_2202


title: SpleenTRNurr77Low_rep1_2205


title: SpleenTRNurr77Low_rep1_2200


title: SpleenTRNurr77Low_rep1_2199


title: SpleenTRNurr77Low_rep1_2222


title: SpleenTRNurr77Low_rep1_2198


title: SpleenTRNurr77Low_rep1_2194


title: SpleenTRNurr77Low_rep1_2193


title: SpleenTRNurr77Low_rep1_2191


title: SpleenTRNurr77Low_rep1_2188


title: SpleenTRNurr77Low_rep1_2190


title: SpleenTRNurr77Low_rep1_2189


title: SpleenTRNurr77Low_rep1_2192


title: SpleenTRNurr77Low_rep1_2196


title: SpleenTRNurr77Low_rep1_2195


title: SpleenTRNurr77Low_rep1_2197


title: SpleenTRNurr77Low_rep1_2185


title: SpleenTRNurr77Low_rep1_2183


title: SpleenTRNurr77Low_rep1_2201


title: SpleenTRNurr77Low_rep1_2210


title: SpleenTRNurr77Low_rep1_2209


title: SpleenTRNurr77Low_rep1_2182


title: SpleenTRNurr77Low_rep1_2181


title: SpleenTRNurr77Low_rep1_2180


title: SpleenTRNurr77Low_rep1_2186


title: SpleenTRNurr77Low_rep1_2184


title: SpleenTRNurr77Low_rep1_2206


title: SpleenTRNurr77Low_rep1_2177


title: SpleenTRNurr77Low_rep1_2179


title: SpleenTRNurr77Low_rep1_2175


title: SpleenTRNurr77Low_rep1_2174


title: SpleenTRNurr77Low_rep1_2176


title: SpleenTRNurr77Low_rep1_2178


title: SpleenTRNurr77Low_rep1_2171


title: SpleenTRNurr77Low_rep1_2162


title: SpleenTRNurr77Low_rep1_2167


title: SpleenTRNurr77Low_rep1_2187


title: SpleenTRNurr77Low_rep1_2173


title: SpleenTRNurr77Low_rep1_2161


title: SpleenTRNurr77Low_rep1_2170


title: SpleenTRNurr77Low_rep1_2169


title: SpleenTRNurr77Low_rep1_2165


title: SpleenTRNurr77Low_rep1_2172


title: SpleenTRNurr77Low_rep1_2168


title: SpleenTRNurr77Low_rep1_2166


title: SpleenTRNurr77Low_rep1_2156


title: SpleenTRNurr77Low_rep1_2160


title: SpleenTRNurr77Low_rep1_2150


title: SpleenTRNurr77Low_rep1_2163


title: SpleenTRNurr77Low_rep1_2159


title: SpleenTRNurr77Low_rep1_2154


title: SpleenTRNurr77Low_rep1_2149


title: SpleenTRNurr77Low_rep1_2158


title: SpleenTRNurr77Low_rep1_2147


title: SpleenTRNurr77Low_rep1_2145


title: SpleenTRNurr77Low_rep1_2146


title: SpleenTRNurr77Low_rep1_2155


title: SpleenTRNurr77Low_rep1_2153


title: SpleenTRNurr77Low_rep1_2144


title: SpleenTRNurr77Low_rep1_2143


title: SpleenTRNurr77Low_rep1_2152


title: SpleenTRNurr77Low_rep1_2157


title: SpleenTRNurr77Low_rep1_2164


title: SpleenTRNurr77Low_rep1_2151


title: SpleenTRNurr77Low_rep1_2140


title: SpleenTRNurr77Low_rep1_2142


title: SpleenTRNurr77Low_rep1_2141


title: SpleenTRNurr77Low_rep1_2138


title: SpleenTRNurr77Low_rep1_2139


title: SpleenTRNurr77Low_rep1_2137


title: SpleenTRNurr77Low_rep1_2133


title: SpleenTRNurr77Low_rep1_2135


title: SpleenTRNurr77Low_rep1_2134


title: SpleenTRNurr77Low_rep1_2148


title: SpleenTRNurr77Low_rep1_2130


title: SpleenTRNurr77Low_rep1_2129


title: SpleenTRNurr77Low_rep1_2128


title: SpleenTRNurr77Low_rep1_2127


title: SpleenTRNurr77Low_rep1_2126


title: SpleenTRNurr77Low_rep1_2124


title: SpleenTRNurr77Low_rep1_2122


title: SpleenTRNurr77Low_rep1_2125


title: SpleenTRNurr77Low_rep1_2123


title: SpleenTRNurr77Low_rep1_2118


title: SpleenTRNurr77Low_rep1_2119


title: SpleenTRNurr77Low_rep1_2114


title: SpleenTRNurr77Low_rep1_2117


title: SpleenTRNurr77Low_rep1_2116


title: SpleenTRNurr77Low_rep1_2121


title: SpleenTRNurr77Low_rep1_2115


title: SpleenTRNurr77Low_rep1_2110


title: SpleenTRNurr77Low_rep1_2108


title: SpleenTRNurr77Low_rep1_2132


title: SpleenTRNurr77Low_rep1_2113


title: SpleenTRNurr77Low_rep1_2136


title: SpleenTRNurr77Low_rep1_2111


title: SpleenTRNurr77Low_rep1_2131


title: SpleenTRNurr77Low_rep1_2112


title: SpleenTRNurr77Low_rep1_2105


title: SpleenTRNurr77Low_rep1_2104


title: SpleenTRNurr77Low_rep1_2107


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title: SpleenTRNurr77Low_rep1_1672


title: SpleenTRNurr77Low_rep1_1675


title: SpleenTRNurr77Low_rep1_1671


title: SpleenTRNurr77Low_rep1_1670


title: SpleenTRNurr77Low_rep1_1669


title: SpleenTRNurr77Low_rep1_1668


title: SpleenTRNurr77Low_rep1_1662


title: SpleenTRNurr77Low_rep1_1666


title: SpleenTRNurr77Low_rep1_1664


title: SpleenTRNurr77Low_rep1_1660


title: SpleenTRNurr77Low_rep1_1661


title: SpleenTRNurr77Low_rep1_1667


title: SpleenTRNurr77Low_rep1_1657


title: SpleenTRNurr77Low_rep1_1655


title: SpleenTRNurr77Low_rep1_1659


title: SpleenTRNurr77Low_rep1_1658


title: SpleenTRNurr77Low_rep1_1653


title: SpleenTRNurr77Low_rep1_1652


title: SpleenTRNurr77Low_rep1_1651


title: SpleenTRNurr77Low_rep1_1649


title: SpleenTRNurr77Low_rep1_1650


title: SpleenTRNurr77Low_rep1_1648


title: SpleenTRNurr77Low_rep1_1647


title: SpleenTRNurr77Low_rep1_1645


title: SpleenTRNurr77Low_rep1_1663


title: SpleenTRNurr77Low_rep1_1643


title: SpleenTRNurr77Low_rep1_1644


title: SpleenTRNurr77Low_rep1_1646


title: SpleenTRNurr77Low_rep1_1640


title: SpleenTRNurr77Low_rep1_1641


title: SpleenTRNurr77Low_rep1_1665


title: SpleenTRNurr77Low_rep1_1638


title: SpleenTRNurr77Low_rep1_1656


title: SpleenTRNurr77Low_rep1_1634


title: SpleenTRNurr77Low_rep1_1636


title: SpleenTRNurr77Low_rep1_1639


title: SpleenTRNurr77Low_rep1_1637


title: SpleenTRNurr77Low_rep1_1635


title: SpleenTRNurr77Low_rep1_1633


title: SpleenTRNurr77Low_rep1_1654


title: SpleenTRNurr77Low_rep1_1632


title: SpleenTRNurr77Low_rep1_1629


title: SpleenTRNurr77Low_rep1_1627


title: SpleenTRNurr77Low_rep1_1631


title: SpleenTRNurr77Low_rep1_1628


title: SpleenTRNurr77Low_rep1_1622


title: SpleenTRNurr77Low_rep1_1621


title: SpleenTRNurr77Low_rep1_1626


title: SpleenTRNurr77Low_rep1_1642


title: SpleenTRNurr77Low_rep1_1624


title: SpleenTRNurr77Low_rep1_1620


title: SpleenTRNurr77Low_rep1_1618


title: SpleenTRNurr77Low_rep1_1619


title: SpleenTRNurr77Low_rep1_1615


title: SpleenTRNurr77Low_rep1_1617


title: SpleenTRNurr77Low_rep1_1616


title: SpleenTRNurr77Low_rep1_1614


title: SpleenTRNurr77Low_rep1_1611


title: SpleenTRNurr77Low_rep1_1612


title: SpleenTRNurr77Low_rep1_1613


title: SpleenTRNurr77Low_rep1_1610


title: SpleenTRNurr77Low_rep1_1625


title: SpleenTRNurr77Low_rep1_1608