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title: BloodGT1923

status: Public on Feb 19 2018

type: genomic

city: Wageningen

first: Veronika

last: Laine

address: Droevendaalsesteeg 10

department: Animal Ecology

country: Netherlands

institute: NIOO-KNAW

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hyb_protocol: DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.

scan_protocol: The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.

data_processing:
A total of 3.116 great tits were genotyped using a custom made Affymetrix® great tit 650K SNP chip at Edinburgh Genomics (Edinburgh, United Kingdom). SNP calling was done following the Affymetrix® best practices workflow by using the Axiom® Analysis Suite 1.1.
The processed data file "SamValuesAll.txt" includes the Genotype Call (SNP call): AA (0), AB (1), BB (2), and NoCall (3); Columns with header 'A' or 'B' represent the raw signal intensity from the probes targeting each allele (i.e. A and B).

label: Biotin

molecule: genomic DNA

organism: Parus major

source_name: wild

label_protocol: As per manufacturer (Affymetrix)

extract_protocol: Whole blood samples were stored in either 1 ml Cell Lysis Solution (Gentra Puregene Kit, Qiagen, USA) or Queens buffer. DNA was extracted by using the FavorPrep 96-Well Genomic DNA Extraction Kit (Favorgen Biotech corp.). DNA quality and DNA concentration were measured on a Nanodrop 2000 (Thermo Scientific).

tag: tissue

value: blood

tag: Sex

value: female


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