title: SpleenTRNurr77Med_rep1_591


title: SpleenTRNurr77Med_rep1_594

status: Public on Feb 16 2018

type: SRA

city: Boston

first: CBDM

last: Lab

state: MA

address: 77 Avenue Louis Pasteur

department: Microbiology and Immunobiology

country: USA

institute: Harvard Medical School

library_source: transcriptomic

data_processing:
fastq quality read filtering (>80% with Sanger Q>20)
cell barcode demultiplexing (custom script)
mapping with tophat2 on the mm10 transcriptome
filtering out low alignment score reads and multiple mapping reads (samtools)
duplication filtering using unique molecular identifiers (custom scripts). UMI sequence is at the 4rth position in the read ID (separation by ":")
Genome_build: mm10
Supplementary_files_format_and_content: raw read counts

molecule: total RNA

organism: Mus musculus

source_name: spleen

growth_protocol: Live cells were sorted by flow cytometry from the spleen of mice (carrying Foxp3 and Nr4a1 reporter genes).

extract_protocol:
Spleen were mechanically dissociated to single cell suspensions. Tregs were sorted by flow cytometry as DAPI- TCRb+ CD4+ FoxP3-Thy1.1+ cells in 3 different bins of Nr4a1-GFP expression (low=0-20%, med=40-60%, high=80-100%). Single cells were then encapsulated in nanodroplets according to the InDrops protocol.
mRNA was reverse transcribed using adapters containing oligo dT in each droplet. The emulsion was then broken and linear amplification was performed by in vitro transcription. Illumina adapters were added by reverse transcription and PCR. Library were sequenced using a NextSeq500.

tag: tissue

value: spleen

tag: library name

tag: sample type

tag: strain

value: B6 Nr4a1-Gfp Foxp3-Ires-Thy1.1

tag: Sex

value: male

tag: age (months)

tag: total number of reads

tag: number of mapped reads

tag: number of unique reads (umi)


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title: SpleenTRNurr77High_rep1_1780


title: SpleenTRNurr77High_rep1_1777


title: SpleenTRNurr77High_rep1_1776


title: SpleenTRNurr77High_rep1_1779


title: SpleenTRNurr77High_rep1_1774


title: SpleenTRNurr77High_rep1_1775


title: SpleenTRNurr77High_rep1_1770


title: SpleenTRNurr77High_rep1_1771


title: SpleenTRNurr77High_rep1_1772


title: SpleenTRNurr77High_rep1_1773


title: SpleenTRNurr77High_rep1_1790


title: SpleenTRNurr77High_rep1_1769


title: SpleenTRNurr77High_rep1_1763


title: SpleenTRNurr77High_rep1_1766


title: SpleenTRNurr77High_rep1_1784


title: SpleenTRNurr77High_rep1_1785


title: SpleenTRNurr77High_rep1_1765


title: SpleenTRNurr77High_rep1_1764


title: SpleenTRNurr77High_rep1_1762


title: SpleenTRNurr77High_rep1_1761


title: SpleenTRNurr77High_rep1_1760


title: SpleenTRNurr77High_rep1_1757


title: SpleenTRNurr77High_rep1_1759


title: SpleenTRNurr77High_rep1_1755


title: SpleenTRNurr77High_rep1_1758


title: SpleenTRNurr77High_rep1_1756


title: SpleenTRNurr77High_rep1_1778


title: SpleenTRNurr77High_rep1_1753


title: SpleenTRNurr77High_rep1_1749


title: SpleenTRNurr77High_rep1_1767


title: SpleenTRNurr77High_rep1_1752


title: SpleenTRNurr77High_rep1_1751


title: SpleenTRNurr77High_rep1_1748


title: SpleenTRNurr77High_rep1_1747


title: SpleenTRNurr77High_rep1_1746


title: SpleenTRNurr77High_rep1_1745


title: SpleenTRNurr77High_rep1_1768


title: SpleenTRNurr77High_rep1_1742


title: SpleenTRNurr77High_rep1_1744


title: SpleenTRNurr77High_rep1_1743


title: SpleenTRNurr77High_rep1_1738


title: SpleenTRNurr77High_rep1_1737


title: SpleenTRNurr77High_rep1_1736


title: SpleenTRNurr77High_rep1_1740


title: SpleenTRNurr77High_rep1_1754


title: SpleenTRNurr77High_rep1_1750


title: SpleenTRNurr77High_rep1_1731


title: SpleenTRNurr77High_rep1_1735


title: SpleenTRNurr77High_rep1_1732


title: SpleenTRNurr77High_rep1_1733


title: SpleenTRNurr77High_rep1_1728


title: SpleenTRNurr77High_rep1_1730


title: SpleenTRNurr77High_rep1_1729


title: SpleenTRNurr77High_rep1_1725


title: SpleenTRNurr77High_rep1_1727


title: SpleenTRNurr77High_rep1_1723


title: SpleenTRNurr77High_rep1_1739


title: SpleenTRNurr77High_rep1_1721


title: SpleenTRNurr77High_rep1_1741


title: SpleenTRNurr77High_rep1_1720


title: SpleenTRNurr77High_rep1_1717


title: SpleenTRNurr77High_rep1_1726


title: SpleenTRNurr77High_rep1_1715


title: SpleenTRNurr77High_rep1_1719


title: SpleenTRNurr77High_rep1_1716


title: SpleenTRNurr77High_rep1_1714


title: SpleenTRNurr77High_rep1_1734


title: SpleenTRNurr77High_rep1_1713


title: SpleenTRNurr77High_rep1_1710


title: SpleenTRNurr77High_rep1_1704


title: SpleenTRNurr77High_rep1_1712


title: SpleenTRNurr77High_rep1_1724


title: SpleenTRNurr77High_rep1_1708


title: SpleenTRNurr77High_rep1_1705


title: SpleenTRNurr77High_rep1_1702


title: SpleenTRNurr77High_rep1_1703


title: SpleenTRNurr77High_rep1_1709


title: SpleenTRNurr77High_rep1_1700


title: SpleenTRNurr77High_rep1_1718


title: SpleenTRNurr77High_rep1_1698


title: SpleenTRNurr77High_rep1_1701


title: SpleenTRNurr77High_rep1_1697


title: SpleenTRNurr77High_rep1_1695


title: SpleenTRNurr77High_rep1_1711


title: SpleenTRNurr77High_rep1_1696


title: SpleenTRNurr77High_rep1_1690


title: SpleenTRNurr77High_rep1_1699


title: SpleenTRNurr77High_rep1_1691


title: SpleenTRNurr77High_rep1_1693


title: SpleenTRNurr77High_rep1_1692


title: SpleenTRNurr77High_rep1_1688


title: SpleenTRNurr77High_rep1_1694


title: SpleenTRNurr77High_rep1_1689


title: SpleenTRNurr77High_rep1_1707


title: SpleenTRNurr77High_rep1_1687


title: SpleenTRNurr77High_rep1_1686


title: SpleenTRNurr77High_rep1_1685


title: SpleenTRNurr77High_rep1_1682


title: SpleenTRNurr77High_rep1_1681


title: SpleenTRNurr77High_rep1_1678


title: SpleenTRNurr77High_rep1_1680


title: SpleenTRNurr77High_rep1_1706


title: SpleenTRNurr77High_rep1_1676


title: SpleenTRNurr77High_rep1_1675


title: SpleenTRNurr77High_rep1_1673


title: SpleenTRNurr77High_rep1_1671


title: SpleenTRNurr77High_rep1_1672


title: SpleenTRNurr77High_rep1_1674


title: SpleenTRNurr77High_rep1_1668


title: SpleenTRNurr77High_rep1_1670


title: SpleenTRNurr77High_rep1_1666


title: SpleenTRNurr77High_rep1_1667


title: SpleenTRNurr77High_rep1_1664


title: SpleenTRNurr77High_rep1_1663


title: SpleenTRNurr77High_rep1_1669


title: SpleenTRNurr77High_rep1_1665


title: SpleenTRNurr77High_rep1_1661


title: SpleenTRNurr77High_rep1_1660


title: SpleenTRNurr77High_rep1_1683


title: SpleenTRNurr77High_rep1_1684


title: SpleenTRNurr77High_rep1_1659


title: SpleenTRNurr77High_rep1_1679


title: SpleenTRNurr77High_rep1_1657


title: SpleenTRNurr77High_rep1_1658


title: SpleenTRNurr77High_rep1_1655


title: SpleenTRNurr77High_rep1_1652


title: SpleenTRNurr77High_rep1_1656


title: SpleenTRNurr77High_rep1_1651


title: SpleenTRNurr77High_rep1_1677


title: SpleenTRNurr77High_rep1_1654


title: SpleenTRNurr77High_rep1_1648


title: SpleenTRNurr77High_rep1_1649


title: SpleenTRNurr77High_rep1_1647


title: SpleenTRNurr77High_rep1_1650


title: SpleenTRNurr77High_rep1_1662


title: SpleenTRNurr77High_rep1_1646


title: SpleenTRNurr77High_rep1_1639


title: SpleenTRNurr77High_rep1_1644


title: SpleenTRNurr77High_rep1_1638


title: SpleenTRNurr77High_rep1_1642


title: SpleenTRNurr77High_rep1_1640


title: SpleenTRNurr77High_rep1_1641


title: SpleenTRNurr77High_rep1_1633


title: SpleenTRNurr77High_rep1_1653


title: SpleenTRNurr77High_rep1_1637


title: SpleenTRNurr77High_rep1_1635


title: SpleenTRNurr77High_rep1_1636


title: SpleenTRNurr77High_rep1_1627


title: SpleenTRNurr77High_rep1_1632


title: SpleenTRNurr77High_rep1_1625


title: SpleenTRNurr77High_rep1_1634


title: SpleenTRNurr77High_rep1_1630


title: SpleenTRNurr77High_rep1_1623


title: SpleenTRNurr77High_rep1_1631


title: SpleenTRNurr77High_rep1_1628


title: SpleenTRNurr77High_rep1_1629


title: SpleenTRNurr77High_rep1_1622


title: SpleenTRNurr77High_rep1_1624


title: SpleenTRNurr77High_rep1_1643


title: SpleenTRNurr77High_rep1_1617


title: SpleenTRNurr77High_rep1_1645


title: SpleenTRNurr77High_rep1_1620


title: SpleenTRNurr77High_rep1_1621


title: SpleenTRNurr77High_rep1_1619


title: SpleenTRNurr77High_rep1_1618


title: SpleenTRNurr77High_rep1_1613


title: SpleenTRNurr77High_rep1_1614


title: SpleenTRNurr77High_rep1_1616


title: SpleenTRNurr77High_rep1_1610


title: SpleenTRNurr77High_rep1_1611


title: SpleenTRNurr77High_rep1_1615


title: SpleenTRNurr77High_rep1_1608


title: SpleenTRNurr77High_rep1_1609


title: SpleenTRNurr77High_rep1_1626


title: SpleenTRNurr77High_rep1_1606


title: SpleenTRNurr77High_rep1_1603


title: SpleenTRNurr77High_rep1_1604


title: SpleenTRNurr77High_rep1_1602


title: SpleenTRNurr77High_rep1_1600


title: SpleenTRNurr77High_rep1_1605


title: SpleenTRNurr77High_rep1_1599


title: SpleenTRNurr77High_rep1_1598


title: SpleenTRNurr77High_rep1_1593


title: SpleenTRNurr77High_rep1_1595


title: SpleenTRNurr77High_rep1_1590


title: SpleenTRNurr77High_rep1_1594


title: SpleenTRNurr77High_rep1_1612


title: SpleenTRNurr77High_rep1_1588


title: SpleenTRNurr77High_rep1_1592


title: SpleenTRNurr77High_rep1_1591


title: SpleenTRNurr77High_rep1_1587


title: SpleenTRNurr77High_rep1_1583


title: SpleenTRNurr77High_rep1_1589


title: SpleenTRNurr77High_rep1_1607


title: SpleenTRNurr77High_rep1_1584


title: SpleenTRNurr77High_rep1_1582


title: SpleenTRNurr77High_rep1_1585


title: SpleenTRNurr77High_rep1_1579


title: SpleenTRNurr77High_rep1_1580


title: SpleenTRNurr77High_rep1_1581


title: SpleenTRNurr77High_rep1_1596


title: SpleenTRNurr77High_rep1_1578


title: SpleenTRNurr77High_rep1_1597


title: SpleenTRNurr77High_rep1_1601


title: SpleenTRNurr77High_rep1_1574


title: SpleenTRNurr77High_rep1_1576


title: SpleenTRNurr77High_rep1_1573


title: SpleenTRNurr77High_rep1_1722


title: SpleenTRNurr77High_rep1_1586


title: SpleenTRNurr77High_rep1_1572


title: SpleenTRNurr77High_rep1_1571


title: SpleenTRNurr77High_rep1_1570


title: SpleenTRNurr77High_rep1_1569


title: SpleenTRNurr77High_rep1_1567


title: SpleenTRNurr77High_rep1_1577


title: SpleenTRNurr77High_rep1_1563


title: SpleenTRNurr77High_rep1_1565


title: SpleenTRNurr77High_rep1_1564


title: SpleenTRNurr77High_rep1_1562


title: SpleenTRNurr77High_rep1_1566


title: SpleenTRNurr77High_rep1_1561


title: SpleenTRNurr77High_rep1_1558


title: SpleenTRNurr77High_rep1_1559


title: SpleenTRNurr77High_rep1_1560


title: SpleenTRNurr77High_rep1_1555


title: SpleenTRNurr77High_rep1_1557


title: SpleenTRNurr77High_rep1_1553


title: SpleenTRNurr77High_rep1_1575


title: SpleenTRNurr77High_rep1_1554


title: SpleenTRNurr77High_rep1_1556


title: SpleenTRNurr77High_rep1_1551


title: SpleenTRNurr77High_rep1_1548


title: SpleenTRNurr77High_rep1_1550


title: SpleenTRNurr77High_rep1_1549


title: SpleenTRNurr77High_rep1_1552


title: SpleenTRNurr77High_rep1_1547


title: SpleenTRNurr77High_rep1_1568


title: SpleenTRNurr77High_rep1_1545


title: SpleenTRNurr77High_rep1_1546


title: SpleenTRNurr77High_rep1_1544


title: SpleenTRNurr77High_rep1_1541


title: SpleenTRNurr77High_rep1_1539


title: SpleenTRNurr77High_rep1_1542


title: SpleenTRNurr77High_rep1_1543


title: SpleenTRNurr77High_rep1_1540


title: SpleenTRNurr77High_rep1_1536


title: SpleenTRNurr77High_rep1_1534


title: SpleenTRNurr77High_rep1_1538


title: SpleenTRNurr77High_rep1_1537


title: SpleenTRNurr77High_rep1_1535


title: SpleenTRNurr77High_rep1_1531


title: SpleenTRNurr77High_rep1_1533


title: SpleenTRNurr77High_rep1_1528


title: SpleenTRNurr77High_rep1_1530


title: SpleenTRNurr77High_rep1_1524


title: SpleenTRNurr77High_rep1_1529


title: SpleenTRNurr77High_rep1_1527


title: SpleenTRNurr77High_rep1_1526


title: SpleenTRNurr77High_rep1_1523


title: SpleenTRNurr77High_rep1_1532


title: SpleenTRNurr77High_rep1_1522


title: SpleenTRNurr77High_rep1_1520


title: SpleenTRNurr77High_rep1_1521


title: SpleenTRNurr77High_rep1_1518


title: SpleenTRNurr77High_rep1_1517


title: SpleenTRNurr77High_rep1_1519


title: SpleenTRNurr77High_rep1_1516


title: SpleenTRNurr77High_rep1_1513


title: SpleenTRNurr77High_rep1_1511


title: SpleenTRNurr77High_rep1_1510


title: SpleenTRNurr77High_rep1_1512


title: SpleenTRNurr77High_rep1_1509


title: SpleenTRNurr77High_rep1_1508


title: SpleenTRNurr77High_rep1_1514


title: SpleenTRNurr77High_rep1_1506


title: SpleenTRNurr77High_rep1_1507


title: SpleenTRNurr77High_rep1_1505


title: SpleenTRNurr77High_rep1_1504


title: SpleenTRNurr77High_rep1_1525


title: SpleenTRNurr77High_rep1_1501


title: SpleenTRNurr77High_rep1_1499


title: SpleenTRNurr77High_rep1_1497


title: SpleenTRNurr77High_rep1_1495


title: SpleenTRNurr77High_rep1_1498


title: SpleenTRNurr77High_rep1_1494


title: SpleenTRNurr77High_rep1_1500


title: SpleenTRNurr77High_rep1_1496


title: SpleenTRNurr77High_rep1_1493


title: SpleenTRNurr77High_rep1_1515


title: SpleenTRNurr77High_rep1_1490


title: SpleenTRNurr77High_rep1_1491


title: SpleenTRNurr77High_rep1_1492


title: SpleenTRNurr77High_rep1_1488


title: SpleenTRNurr77High_rep1_1489


title: SpleenTRNurr77High_rep1_1487


title: SpleenTRNurr77High_rep1_1483


title: SpleenTRNurr77High_rep1_1485


title: SpleenTRNurr77High_rep1_1503


title: SpleenTRNurr77High_rep1_1486


title: SpleenTRNurr77High_rep1_1484


title: SpleenTRNurr77High_rep1_1502


title: SpleenTRNurr77High_rep1_1476


title: SpleenTRNurr77High_rep1_1481


title: SpleenTRNurr77High_rep1_1475


title: SpleenTRNurr77High_rep1_1474


title: SpleenTRNurr77High_rep1_1480


title: SpleenTRNurr77High_rep1_1482


title: SpleenTRNurr77High_rep1_1477


title: SpleenTRNurr77High_rep1_1479


title: SpleenTRNurr77High_rep1_1473


title: SpleenTRNurr77High_rep1_1471


title: SpleenTRNurr77High_rep1_1472


title: SpleenTRNurr77High_rep1_1467


title: SpleenTRNurr77High_rep1_1468


title: SpleenTRNurr77High_rep1_1469


title: SpleenTRNurr77High_rep1_1470


title: SpleenTRNurr77High_rep1_1462


title: SpleenTRNurr77High_rep1_1465


title: SpleenTRNurr77High_rep1_1463


title: SpleenTRNurr77High_rep1_1464


title: SpleenTRNurr77High_rep1_1461


title: SpleenTRNurr77High_rep1_1459


title: SpleenTRNurr77High_rep1_1460


title: SpleenTRNurr77High_rep1_1455


title: SpleenTRNurr77High_rep1_1478


title: SpleenTRNurr77High_rep1_1453


title: SpleenTRNurr77High_rep1_1457


title: SpleenTRNurr77High_rep1_1458


title: SpleenTRNurr77High_rep1_1454


title: SpleenTRNurr77High_rep1_1448


title: SpleenTRNurr77High_rep1_1456


title: SpleenTRNurr77High_rep1_1449


title: SpleenTRNurr77High_rep1_1450


title: SpleenTRNurr77High_rep1_1446


title: SpleenTRNurr77High_rep1_1443


title: SpleenTRNurr77High_rep1_1466


title: SpleenTRNurr77High_rep1_1447


title: SpleenTRNurr77High_rep1_1442


title: SpleenTRNurr77High_rep1_1441


title: SpleenTRNurr77High_rep1_1440


title: SpleenTRNurr77High_rep1_1435


title: SpleenTRNurr77High_rep1_1444


title: SpleenTRNurr77High_rep1_1439


title: SpleenTRNurr77High_rep1_1437


title: SpleenTRNurr77High_rep1_1438


title: SpleenTRNurr77High_rep1_1434


title: SpleenTRNurr77High_rep1_1431


title: SpleenTRNurr77High_rep1_1432


title: SpleenTRNurr77High_rep1_1433


title: SpleenTRNurr77High_rep1_1436


title: SpleenTRNurr77High_rep1_1429


title: SpleenTRNurr77High_rep1_1430


title: SpleenTRNurr77High_rep1_1428


title: SpleenTRNurr77High_rep1_1452


title: SpleenTRNurr77High_rep1_1445


title: SpleenTRNurr77High_rep1_1451


title: SpleenTRNurr77High_rep1_1426


title: SpleenTRNurr77High_rep1_1425


title: SpleenTRNurr77High_rep1_1427


title: SpleenTRNurr77High_rep1_1420


title: SpleenTRNurr77High_rep1_1423


title: SpleenTRNurr77High_rep1_1422


title: SpleenTRNurr77High_rep1_1419


title: SpleenTRNurr77High_rep1_1421


title: SpleenTRNurr77High_rep1_1417


title: SpleenTRNurr77High_rep1_1418


title: SpleenTRNurr77High_rep1_1416


title: SpleenTRNurr77High_rep1_1414


title: SpleenTRNurr77High_rep1_1410


title: SpleenTRNurr77High_rep1_1413


title: SpleenTRNurr77High_rep1_1407


title: SpleenTRNurr77High_rep1_1415


title: SpleenTRNurr77High_rep1_1409


title: SpleenTRNurr77High_rep1_1408


title: SpleenTRNurr77High_rep1_1405


title: SpleenTRNurr77High_rep1_1404


title: SpleenTRNurr77High_rep1_1411


title: SpleenTRNurr77High_rep1_1424


title: SpleenTRNurr77High_rep1_1402


title: SpleenTRNurr77High_rep1_1400


title: SpleenTRNurr77High_rep1_1406


title: SpleenTRNurr77High_rep1_1401


title: SpleenTRNurr77High_rep1_1399


title: SpleenTRNurr77High_rep1_1403


title: SpleenTRNurr77High_rep1_1397


title: SpleenTRNurr77High_rep1_1395


title: SpleenTRNurr77High_rep1_1396


title: SpleenTRNurr77High_rep1_1398


title: SpleenTRNurr77High_rep1_1390


title: SpleenTRNurr77High_rep1_1388


title: SpleenTRNurr77High_rep1_1389


title: SpleenTRNurr77High_rep1_1391


title: SpleenTRNurr77High_rep1_1392


title: SpleenTRNurr77High_rep1_1412


title: SpleenTRNurr77High_rep1_1393


title: SpleenTRNurr77High_rep1_1382


title: SpleenTRNurr77High_rep1_1381


title: SpleenTRNurr77High_rep1_1387


title: SpleenTRNurr77High_rep1_1378


title: SpleenTRNurr77High_rep1_1386


title: SpleenTRNurr77High_rep1_1374


title: SpleenTRNurr77High_rep1_1384


title: SpleenTRNurr77High_rep1_1373


title: SpleenTRNurr77High_rep1_1377


title: SpleenTRNurr77High_rep1_1385


title: SpleenTRNurr77High_rep1_1376


title: SpleenTRNurr77High_rep1_1371


title: SpleenTRNurr77High_rep1_1394


title: SpleenTRNurr77High_rep1_1383


title: SpleenTRNurr77High_rep1_1372


title: SpleenTRNurr77High_rep1_1375


title: SpleenTRNurr77High_rep1_1367


title: SpleenTRNurr77High_rep1_1366


title: SpleenTRNurr77High_rep1_1379


title: SpleenTRNurr77High_rep1_1363


title: SpleenTRNurr77High_rep1_1370


title: SpleenTRNurr77High_rep1_1362


title: SpleenTRNurr77High_rep1_1369


title: SpleenTRNurr77High_rep1_1357


title: SpleenTRNurr77High_rep1_1356


title: SpleenTRNurr77High_rep1_1355


title: SpleenTRNurr77High_rep1_1368


title: SpleenTRNurr77High_rep1_1354


title: SpleenTRNurr77High_rep1_1351


title: SpleenTRNurr77High_rep1_1348


title: SpleenTRNurr77High_rep1_1380


title: SpleenTRNurr77High_rep1_1365


title: SpleenTRNurr77High_rep1_1344


title: SpleenTRNurr77High_rep1_1347


title: SpleenTRNurr77High_rep1_1364


title: SpleenTRNurr77High_rep1_1353


title: SpleenTRNurr77High_rep1_1358


title: SpleenTRNurr77High_rep1_1352


title: SpleenTRNurr77High_rep1_1361


title: SpleenTRNurr77High_rep1_1360


title: SpleenTRNurr77High_rep1_1359


title: SpleenTRNurr77High_rep1_1339


title: SpleenTRNurr77High_rep1_1335


title: SpleenTRNurr77High_rep1_1346


title: SpleenTRNurr77High_rep1_1332


title: SpleenTRNurr77High_rep1_1350


title: SpleenTRNurr77High_rep1_1331


title: SpleenTRNurr77High_rep1_1342


title: SpleenTRNurr77High_rep1_1330


title: SpleenTRNurr77High_rep1_1345


title: SpleenTRNurr77High_rep1_1334


title: SpleenTRNurr77High_rep1_1338


title: SpleenTRNurr77High_rep1_1328


title: SpleenTRNurr77High_rep1_1340


title: SpleenTRNurr77High_rep1_1343


title: SpleenTRNurr77High_rep1_1322


title: SpleenTRNurr77High_rep1_1341


title: SpleenTRNurr77High_rep1_1349


title: SpleenTRNurr77High_rep1_1325


title: SpleenTRNurr77High_rep1_1327


title: SpleenTRNurr77High_rep1_1324


title: SpleenTRNurr77High_rep1_1326


title: SpleenTRNurr77High_rep1_1318


title: SpleenTRNurr77High_rep1_1333


title: SpleenTRNurr77High_rep1_1315


title: SpleenTRNurr77High_rep1_1336


title: SpleenTRNurr77High_rep1_1329


title: SpleenTRNurr77High_rep1_1323


title: SpleenTRNurr77High_rep1_1321


title: SpleenTRNurr77High_rep1_1337


title: SpleenTRNurr77High_rep1_1312


title: SpleenTRNurr77High_rep1_1319


title: SpleenTRNurr77High_rep1_1310


title: SpleenTRNurr77High_rep1_1308


title: SpleenTRNurr77High_rep1_1307


title: SpleenTRNurr77High_rep1_1313


title: SpleenTRNurr77High_rep1_1320


title: SpleenTRNurr77High_rep1_1317


title: SpleenTRNurr77High_rep1_1303


title: SpleenTRNurr77High_rep1_1309


title: SpleenTRNurr77High_rep1_1316


title: SpleenTRNurr77High_rep1_1305


title: SpleenTRNurr77High_rep1_1306


title: SpleenTRNurr77High_rep1_1300


title: SpleenTRNurr77High_rep1_1296


title: SpleenTRNurr77High_rep1_1299


title: SpleenTRNurr77High_rep1_1302


title: SpleenTRNurr77High_rep1_1298


title: SpleenTRNurr77High_rep1_1297


title: SpleenTRNurr77High_rep1_1311


title: SpleenTRNurr77High_rep1_1292


title: SpleenTRNurr77High_rep1_1295


title: SpleenTRNurr77High_rep1_1304


title: SpleenTRNurr77High_rep1_1291


title: SpleenTRNurr77High_rep1_1314


title: SpleenTRNurr77High_rep1_1290


title: SpleenTRNurr77High_rep1_1294


title: SpleenTRNurr77High_rep1_1293


title: SpleenTRNurr77High_rep1_1289


title: SpleenTRNurr77High_rep1_1301


title: SpleenTRNurr77High_rep1_1287


title: SpleenTRNurr77High_rep1_1279


title: SpleenTRNurr77High_rep1_1280


title: SpleenTRNurr77High_rep1_1283


title: SpleenTRNurr77High_rep1_1278


title: SpleenTRNurr77High_rep1_1277


title: SpleenTRNurr77High_rep1_1284


title: SpleenTRNurr77High_rep1_1273


title: SpleenTRNurr77High_rep1_1275


title: SpleenTRNurr77High_rep1_1282


title: SpleenTRNurr77High_rep1_1286


title: SpleenTRNurr77High_rep1_1274


title: SpleenTRNurr77High_rep1_1272


title: SpleenTRNurr77High_rep1_1269


title: SpleenTRNurr77High_rep1_1276


title: SpleenTRNurr77High_rep1_1262


title: SpleenTRNurr77High_rep1_1288


title: SpleenTRNurr77High_rep1_1264


title: SpleenTRNurr77High_rep1_1265


title: SpleenTRNurr77High_rep1_1271


title: SpleenTRNurr77High_rep1_1258


title: SpleenTRNurr77High_rep1_1268


title: SpleenTRNurr77High_rep1_1267


title: SpleenTRNurr77High_rep1_1270


title: SpleenTRNurr77High_rep1_1281


title: SpleenTRNurr77High_rep1_1285


title: SpleenTRNurr77High_rep1_1257


title: SpleenTRNurr77High_rep1_1266


title: SpleenTRNurr77High_rep1_1251


title: SpleenTRNurr77High_rep1_1259


title: SpleenTRNurr77High_rep1_1255


title: SpleenTRNurr77High_rep1_1249


title: SpleenTRNurr77High_rep1_1244


title: SpleenTRNurr77High_rep1_1254


title: SpleenTRNurr77High_rep1_1261


title: SpleenTRNurr77High_rep1_1253


title: SpleenTRNurr77High_rep1_1263


title: SpleenTRNurr77High_rep1_1247


title: SpleenTRNurr77High_rep1_1252


title: SpleenTRNurr77High_rep1_1260


title: SpleenTRNurr77High_rep1_1240


title: SpleenTRNurr77High_rep1_1245


title: SpleenTRNurr77High_rep1_1246


title: SpleenTRNurr77High_rep1_1248


title: SpleenTRNurr77High_rep1_1250


title: SpleenTRNurr77High_rep1_1242


title: SpleenTRNurr77High_rep1_1236


title: SpleenTRNurr77High_rep1_1237


title: SpleenTRNurr77High_rep1_1256


title: SpleenTRNurr77High_rep1_1235


title: SpleenTRNurr77High_rep1_1234


title: SpleenTRNurr77High_rep1_1243


title: SpleenTRNurr77High_rep1_1239


title: SpleenTRNurr77High_rep1_1241


title: SpleenTRNurr77High_rep1_1229


title: SpleenTRNurr77High_rep1_1233


title: SpleenTRNurr77High_rep1_1238


title: SpleenTRNurr77High_rep1_1230


title: SpleenTRNurr77High_rep1_1231


title: SpleenTRNurr77High_rep1_1226


title: SpleenTRNurr77High_rep1_1225


title: SpleenTRNurr77High_rep1_1228


title: SpleenTRNurr77High_rep1_1227


title: SpleenTRNurr77High_rep1_1224


title: SpleenTRNurr77High_rep1_1221


title: SpleenTRNurr77High_rep1_1223


title: SpleenTRNurr77High_rep1_1222


title: SpleenTRNurr77High_rep1_1214


title: SpleenTRNurr77High_rep1_1218


title: SpleenTRNurr77High_rep1_1219


title: SpleenTRNurr77High_rep1_1216


title: SpleenTRNurr77High_rep1_1220


title: SpleenTRNurr77High_rep1_1209


title: SpleenTRNurr77High_rep1_1217


title: SpleenTRNurr77High_rep1_1212


title: SpleenTRNurr77High_rep1_1232


title: SpleenTRNurr77High_rep1_1207


title: SpleenTRNurr77High_rep1_1208


title: SpleenTRNurr77High_rep1_1215


title: SpleenTRNurr77High_rep1_1210


title: SpleenTRNurr77High_rep1_1213


title: SpleenTRNurr77High_rep1_1206


title: SpleenTRNurr77High_rep1_1211


title: SpleenTRNurr77High_rep1_1205


title: SpleenTRNurr77High_rep1_1204


title: SpleenTRNurr77High_rep1_1203


title: SpleenTRNurr77High_rep1_1202


title: SpleenTRNurr77High_rep1_1200


title: SpleenTRNurr77High_rep1_1199


title: SpleenTRNurr77High_rep1_1198


title: SpleenTRNurr77High_rep1_1197


title: SpleenTRNurr77High_rep1_1196


title: SpleenTRNurr77High_rep1_1193


title: SpleenTRNurr77High_rep1_1192


title: SpleenTRNurr77High_rep1_1194


title: SpleenTRNurr77High_rep1_1195


title: SpleenTRNurr77High_rep1_1191


title: SpleenTRNurr77High_rep1_1190


title: SpleenTRNurr77High_rep1_1188


title: SpleenTRNurr77High_rep1_1185


title: SpleenTRNurr77High_rep1_1189


title: SpleenTRNurr77High_rep1_1187


title: SpleenTRNurr77High_rep1_1183


title: SpleenTRNurr77High_rep1_1181


title: SpleenTRNurr77High_rep1_1180


title: SpleenTRNurr77High_rep1_1182


title: SpleenTRNurr77High_rep1_1179


title: SpleenTRNurr77High_rep1_1178


title: SpleenTRNurr77High_rep1_1177


title: SpleenTRNurr77High_rep1_1176


title: SpleenTRNurr77High_rep1_1201


title: SpleenTRNurr77High_rep1_1174


title: SpleenTRNurr77High_rep1_1175


title: SpleenTRNurr77High_rep1_1172


title: SpleenTRNurr77High_rep1_1173


title: SpleenTRNurr77High_rep1_1170


title: SpleenTRNurr77High_rep1_1171


title: SpleenTRNurr77High_rep1_1167


title: SpleenTRNurr77High_rep1_1169


title: SpleenTRNurr77High_rep1_1168


title: SpleenTRNurr77High_rep1_1184


title: SpleenTRNurr77High_rep1_1166


title: SpleenTRNurr77High_rep1_1186


title: SpleenTRNurr77High_rep1_1163


title: SpleenTRNurr77High_rep1_1161


title: SpleenTRNurr77High_rep1_1159


title: SpleenTRNurr77High_rep1_1162


title: SpleenTRNurr77High_rep1_1165


title: SpleenTRNurr77High_rep1_1160


title: SpleenTRNurr77High_rep1_1157


title: SpleenTRNurr77High_rep1_1156


title: SpleenTRNurr77High_rep1_1158


title: SpleenTRNurr77High_rep1_1153


title: SpleenTRNurr77High_rep1_1155


title: SpleenTRNurr77High_rep1_1154


title: SpleenTRNurr77High_rep1_1152


title: SpleenTRNurr77High_rep1_1149


title: SpleenTRNurr77High_rep1_1150


title: SpleenTRNurr77High_rep1_1151


title: SpleenTRNurr77High_rep1_1146


title: SpleenTRNurr77High_rep1_1148


title: SpleenTRNurr77High_rep1_1147


title: SpleenTRNurr77High_rep1_1145


title: SpleenTRNurr77High_rep1_1164


title: SpleenTRNurr77High_rep1_1142


title: SpleenTRNurr77High_rep1_1144


title: SpleenTRNurr77High_rep1_1143


title: SpleenTRNurr77High_rep1_1141


title: SpleenTRNurr77High_rep1_1136


title: SpleenTRNurr77High_rep1_1133


title: SpleenTRNurr77High_rep1_1137


title: SpleenTRNurr77High_rep1_1138


title: SpleenTRNurr77High_rep1_1135


title: SpleenTRNurr77High_rep1_1140


title: SpleenTRNurr77High_rep1_1139


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title: SpleenTRNurr77High_rep1_1128


title: SpleenTRNurr77High_rep1_1122


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title: SpleenTRNurr77High_rep1_1123


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title: SpleenTRNurr77High_rep1_1112


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title: SpleenTRNurr77High_rep1_1106


title: SpleenTRNurr77High_rep1_1110


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title: SpleenTRNurr77High_rep1_1103


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title: SpleenTRNurr77High_rep1_1107


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title: SpleenTRNurr77High_rep1_1075


title: SpleenTRNurr77High_rep1_1096


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title: SpleenTRNurr77High_rep1_1038


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title: SpleenTRNurr77High_rep1_1035


title: SpleenTRNurr77High_rep1_1034


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title: SpleenTRNurr77High_rep1_1059


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title: SpleenTRNurr77High_rep1_1024


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title: SpleenTRNurr77High_rep1_1019


title: SpleenTRNurr77High_rep1_1021


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title: SpleenTRNurr77High_rep1_1013


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title: SpleenTRNurr77High_rep1_1017


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title: SpleenTRNurr77High_rep1_1011


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title: SpleenTRNurr77High_rep1_930


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title: SpleenTRNurr77High_rep1_919


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title: SpleenTRNurr77High_rep1_470


title: SpleenTRNurr77High_rep1_472


title: SpleenTRNurr77High_rep1_467


title: SpleenTRNurr77High_rep1_468


title: SpleenTRNurr77High_rep1_471


title: SpleenTRNurr77High_rep1_491


title: SpleenTRNurr77High_rep1_465


title: SpleenTRNurr77High_rep1_464


title: SpleenTRNurr77High_rep1_466


title: SpleenTRNurr77High_rep1_463


title: SpleenTRNurr77High_rep1_461


title: SpleenTRNurr77High_rep1_481


title: SpleenTRNurr77High_rep1_460


title: SpleenTRNurr77High_rep1_457


title: SpleenTRNurr77High_rep1_455


title: SpleenTRNurr77High_rep1_456


title: SpleenTRNurr77High_rep1_462


title: SpleenTRNurr77High_rep1_458


title: SpleenTRNurr77High_rep1_454


title: SpleenTRNurr77High_rep1_469


title: SpleenTRNurr77High_rep1_448


title: SpleenTRNurr77High_rep1_452


title: SpleenTRNurr77High_rep1_451


title: SpleenTRNurr77High_rep1_447


title: SpleenTRNurr77High_rep1_449


title: SpleenTRNurr77High_rep1_450


title: SpleenTRNurr77High_rep1_453


title: SpleenTRNurr77High_rep1_459


title: SpleenTRNurr77High_rep1_444


title: SpleenTRNurr77High_rep1_440


title: SpleenTRNurr77High_rep1_438


title: SpleenTRNurr77High_rep1_445


title: SpleenTRNurr77High_rep1_439


title: SpleenTRNurr77High_rep1_436


title: SpleenTRNurr77High_rep1_435


title: SpleenTRNurr77High_rep1_442


title: SpleenTRNurr77High_rep1_441


title: SpleenTRNurr77High_rep1_434


title: SpleenTRNurr77High_rep1_432


title: SpleenTRNurr77High_rep1_433


title: SpleenTRNurr77High_rep1_446


title: SpleenTRNurr77High_rep1_431


title: SpleenTRNurr77High_rep1_429


title: SpleenTRNurr77High_rep1_430


title: SpleenTRNurr77High_rep1_427


title: SpleenTRNurr77High_rep1_443


title: SpleenTRNurr77High_rep1_426


title: SpleenTRNurr77High_rep1_418


title: SpleenTRNurr77High_rep1_423


title: SpleenTRNurr77High_rep1_419


title: SpleenTRNurr77High_rep1_424


title: SpleenTRNurr77High_rep1_421


title: SpleenTRNurr77High_rep1_437


title: SpleenTRNurr77High_rep1_425


title: SpleenTRNurr77High_rep1_420


title: SpleenTRNurr77High_rep1_414


title: SpleenTRNurr77High_rep1_422


title: SpleenTRNurr77High_rep1_417


title: SpleenTRNurr77High_rep1_428


title: SpleenTRNurr77High_rep1_413


title: SpleenTRNurr77High_rep1_412


title: SpleenTRNurr77High_rep1_415


title: SpleenTRNurr77High_rep1_411


title: SpleenTRNurr77High_rep1_410


title: SpleenTRNurr77High_rep1_409


title: SpleenTRNurr77High_rep1_408


title: SpleenTRNurr77High_rep1_404


title: SpleenTRNurr77High_rep1_406


title: SpleenTRNurr77High_rep1_405


title: SpleenTRNurr77High_rep1_402


title: SpleenTRNurr77High_rep1_403


title: SpleenTRNurr77High_rep1_401


title: SpleenTRNurr77High_rep1_416


title: SpleenTRNurr77High_rep1_400


title: SpleenTRNurr77High_rep1_398


title: SpleenTRNurr77High_rep1_393


title: SpleenTRNurr77High_rep1_392


title: SpleenTRNurr77High_rep1_396


title: SpleenTRNurr77High_rep1_397


title: SpleenTRNurr77High_rep1_394


title: SpleenTRNurr77High_rep1_391


title: SpleenTRNurr77High_rep1_386


title: SpleenTRNurr77High_rep1_387


title: SpleenTRNurr77High_rep1_380


title: SpleenTRNurr77High_rep1_399


title: SpleenTRNurr77High_rep1_395


title: SpleenTRNurr77High_rep1_379


title: SpleenTRNurr77High_rep1_382


title: SpleenTRNurr77High_rep1_389


title: SpleenTRNurr77High_rep1_378


title: SpleenTRNurr77High_rep1_377


title: SpleenTRNurr77High_rep1_384


title: SpleenTRNurr77High_rep1_374


title: SpleenTRNurr77High_rep1_407


title: SpleenTRNurr77High_rep1_376


title: SpleenTRNurr77High_rep1_375


title: SpleenTRNurr77High_rep1_373


title: SpleenTRNurr77High_rep1_383


title: SpleenTRNurr77High_rep1_390


title: SpleenTRNurr77High_rep1_388


title: SpleenTRNurr77High_rep1_381


title: SpleenTRNurr77High_rep1_372


title: SpleenTRNurr77High_rep1_385


title: SpleenTRNurr77High_rep1_367


title: SpleenTRNurr77High_rep1_370


title: SpleenTRNurr77High_rep1_366


title: SpleenTRNurr77High_rep1_363


title: SpleenTRNurr77High_rep1_369


title: SpleenTRNurr77High_rep1_364


title: SpleenTRNurr77High_rep1_365


title: SpleenTRNurr77High_rep1_368


title: SpleenTRNurr77High_rep1_358


title: SpleenTRNurr77High_rep1_360


title: SpleenTRNurr77High_rep1_362


title: SpleenTRNurr77High_rep1_351


title: SpleenTRNurr77High_rep1_354


title: SpleenTRNurr77High_rep1_350


title: SpleenTRNurr77High_rep1_355


title: SpleenTRNurr77High_rep1_346


title: SpleenTRNurr77High_rep1_345


title: SpleenTRNurr77High_rep1_353


title: SpleenTRNurr77High_rep1_347


title: SpleenTRNurr77High_rep1_356


title: SpleenTRNurr77High_rep1_343


title: SpleenTRNurr77High_rep1_357


title: SpleenTRNurr77High_rep1_348


title: SpleenTRNurr77High_rep1_371


title: SpleenTRNurr77High_rep1_342


title: SpleenTRNurr77High_rep1_361


title: SpleenTRNurr77High_rep1_339


title: SpleenTRNurr77High_rep1_359


title: SpleenTRNurr77High_rep1_341


title: SpleenTRNurr77High_rep1_336


title: SpleenTRNurr77High_rep1_340


title: SpleenTRNurr77High_rep1_338


title: SpleenTRNurr77High_rep1_344


title: SpleenTRNurr77High_rep1_349


title: SpleenTRNurr77High_rep1_335


title: SpleenTRNurr77High_rep1_352


title: SpleenTRNurr77High_rep1_331


title: SpleenTRNurr77High_rep1_332


title: SpleenTRNurr77High_rep1_330


title: SpleenTRNurr77High_rep1_334


title: SpleenTRNurr77High_rep1_325


title: SpleenTRNurr77High_rep1_323


title: SpleenTRNurr77High_rep1_329


title: SpleenTRNurr77High_rep1_326


title: SpleenTRNurr77High_rep1_324


title: SpleenTRNurr77High_rep1_321


title: SpleenTRNurr77High_rep1_327


title: SpleenTRNurr77High_rep1_328


title: SpleenTRNurr77High_rep1_320


title: SpleenTRNurr77High_rep1_322


title: SpleenTRNurr77High_rep1_319


title: SpleenTRNurr77High_rep1_318


title: SpleenTRNurr77High_rep1_317


title: SpleenTRNurr77High_rep1_337


title: SpleenTRNurr77High_rep1_333


title: SpleenTRNurr77High_rep1_316


title: SpleenTRNurr77High_rep1_314


title: SpleenTRNurr77High_rep1_315


title: SpleenTRNurr77High_rep1_313


title: SpleenTRNurr77High_rep1_310


title: SpleenTRNurr77High_rep1_308


title: SpleenTRNurr77High_rep1_306


title: SpleenTRNurr77High_rep1_311


title: SpleenTRNurr77High_rep1_309


title: SpleenTRNurr77High_rep1_303


title: SpleenTRNurr77High_rep1_304


title: SpleenTRNurr77High_rep1_312


title: SpleenTRNurr77High_rep1_302


title: SpleenTRNurr77High_rep1_305


title: SpleenTRNurr77High_rep1_300


title: SpleenTRNurr77High_rep1_296


title: SpleenTRNurr77High_rep1_298


title: SpleenTRNurr77High_rep1_297


title: SpleenTRNurr77High_rep1_294


title: SpleenTRNurr77High_rep1_295


title: SpleenTRNurr77High_rep1_301


title: SpleenTRNurr77High_rep1_290


title: SpleenTRNurr77High_rep1_293


title: SpleenTRNurr77High_rep1_288


title: SpleenTRNurr77High_rep1_289


title: SpleenTRNurr77High_rep1_291


title: SpleenTRNurr77High_rep1_286


title: SpleenTRNurr77High_rep1_287


title: SpleenTRNurr77High_rep1_307


title: SpleenTRNurr77High_rep1_283


title: SpleenTRNurr77High_rep1_285


title: SpleenTRNurr77High_rep1_281


title: SpleenTRNurr77High_rep1_280


title: SpleenTRNurr77High_rep1_282


title: SpleenTRNurr77High_rep1_276


title: SpleenTRNurr77High_rep1_278


title: SpleenTRNurr77High_rep1_279


title: SpleenTRNurr77High_rep1_275


title: SpleenTRNurr77High_rep1_299


title: SpleenTRNurr77High_rep1_274


title: SpleenTRNurr77High_rep1_277


title: SpleenTRNurr77High_rep1_292


title: SpleenTRNurr77High_rep1_269


title: SpleenTRNurr77High_rep1_273


title: SpleenTRNurr77High_rep1_268


title: SpleenTRNurr77High_rep1_265


title: SpleenTRNurr77High_rep1_266


title: SpleenTRNurr77High_rep1_262


title: SpleenTRNurr77High_rep1_284


title: SpleenTRNurr77High_rep1_267


title: SpleenTRNurr77High_rep1_271


title: SpleenTRNurr77High_rep1_263


title: SpleenTRNurr77High_rep1_258


title: SpleenTRNurr77High_rep1_260


title: SpleenTRNurr77High_rep1_256


title: SpleenTRNurr77High_rep1_257


title: SpleenTRNurr77High_rep1_259


title: SpleenTRNurr77High_rep1_255


title: SpleenTRNurr77High_rep1_254


title: SpleenTRNurr77High_rep1_270


title: SpleenTRNurr77High_rep1_251


title: SpleenTRNurr77High_rep1_253


title: SpleenTRNurr77High_rep1_252


title: SpleenTRNurr77High_rep1_272


title: SpleenTRNurr77High_rep1_250


title: SpleenTRNurr77High_rep1_249


title: SpleenTRNurr77High_rep1_248


title: SpleenTRNurr77High_rep1_261


title: SpleenTRNurr77High_rep1_264


title: SpleenTRNurr77High_rep1_247


title: SpleenTRNurr77High_rep1_246


title: SpleenTRNurr77High_rep1_245


title: SpleenTRNurr77High_rep1_244


title: SpleenTRNurr77High_rep1_243


title: SpleenTRNurr77High_rep1_242


title: SpleenTRNurr77High_rep1_241


title: SpleenTRNurr77High_rep1_240


title: SpleenTRNurr77High_rep1_238


title: SpleenTRNurr77High_rep1_239


title: SpleenTRNurr77High_rep1_237


title: SpleenTRNurr77High_rep1_235


title: SpleenTRNurr77High_rep1_234


title: SpleenTRNurr77High_rep1_232


title: SpleenTRNurr77High_rep1_233


title: SpleenTRNurr77High_rep1_231


title: Patient M VEH Day 10 repeat 4

type: RNA

city: BETHESDA

first: PING

last: JIN

state: MD

address: 9000 ROCKVILLE PIKE

department: DTM

institute: CC/DTM/NIH

description:
M4 VEH
Gene expression data from Patient M VEH Day 10.

hyb_protocol: Biotinylated cRNA were hybridized on the arrays for 18h according to the manufacturer’s directions.

scan_protocol: Arrays were scanned (Affymetrix 3000 7G) according to manufacturer's instructions.

data_processing: Arrays data were imported into Partek Genomics Suite using RMA normalization after background subtraction. One-way ANOVA was used to identify differentially expressed genes among the 4 T cell subtypes with a significant cut off of pFDR<0.01. Differentially expressed probe sets were selected using a false discovery rate cutoff of 0.01 without specifying a fold change criterion.

label: biotin

organism: Homo sapiens

source_name: PBMCs, VEH, Day 10, Patient M

label_protocol: Biotinylated cRNA were prepared according to the GeneChip 3' IVT PLUS Reagent Kit user guide from 300 ng total RNA (Applied Biosystems).

extract_protocol: Trizol extraction of total RNA was performed according to the manufacturer's instructions.

treatment_protocol:
Whole PBMCs (not CD8-enriched) from 3 patients (Patients C, M, and P) were activated with anti-CD3 and retrovirally-transduced with CD19 CAR and expanded in presence or absence of pharmacologic inhibitor of Akt.
PBMCs were enriched for 1E6 CD8+ T cells using Miltenyi negative selection kit.

tag: starting cell type

value: PBMCs

tag: treatment

value: VEH

tag: time

value: Day 10

tag: enriched cell type

value: CD8+ T cells

tag: patient id

value: M


title: Patient P VEH Day 10 repeat 2

description:
P2 VEH
Gene expression data from Patient P VEH Day 10.

source_name: PBMCs, VEH, Day 10, Patient P

value: P


title: SpleenTRNurr77High_rep1_236


title: SpleenTRNurr77High_rep1_229


title: Patient P AKT Day 10 repeat 3

description:
P3 AKT
Gene expression data from Patient P AKT Day 10.

source_name: PBMCs, AKT, Day 10, Patient P

value: AKT


title: SpleenTRNurr77High_rep1_228


title: Patient M VEH Day 10 repeat 1

description:
M1 VEH
Gene expression data from Patient M VEH Day 10.


title: Patient P AKT Day 10 repeat 4

description:
P4 AKT
Gene expression data from Patient P AKT Day 10.


title: Patient P VEH Day 10 repeat 3

description:
P3 VEH
Gene expression data from Patient P VEH Day 10.


title: Patient M VEH Day 10 repeat 3

description:
M3 VEH
Gene expression data from Patient M VEH Day 10.


title: Patient C VEH Day 10 repeat 3

description:
C3 VEH
Gene expression data from Patient C VEH Day 10.

source_name: PBMCs, VEH, Day 10, Patient C

value: C


title: Patient C VEH Day 10 repeat 4

description:
C4 VEH
Gene expression data from Patient C VEH Day 10.


title: Patient M VEH Day 10 repeat 2

description:
M2 VEH
Gene expression data from Patient M VEH Day 10.


title: Patient P AKT Day 10 repeat 1

description:
P1 AKT
Gene expression data from Patient P AKT Day 10.


title: Patient C AKT Day 10 repeat 4

description:
C4 AKT
Gene expression data from Patient C AKT Day 10.

source_name: PBMCs, AKT, Day 10, Patient C


title: Patient P VEH Day 10 repeat 4

description:
P4 VEH
Gene expression data from Patient P VEH Day 10.


title: Patient P VEH Day 10 repeat 1

description:
P1 VEH
Gene expression data from Patient P VEH Day 10.


title: Patient M AKT Day 10 repeat 3

description:
M3 AKT
Gene expression data from Patient M AKT Day 10.

source_name: PBMCs, AKT, Day 10, Patient M


title: Patient C VEH Day 10 repeat 1

description:
C1 VEH
Gene expression data from Patient C VEH Day 10.


title: Patient M AKT Day 10 repeat 2

description:
M2 AKT
Gene expression data from Patient M AKT Day 10.


title: Patient P AKT Day 10 repeat 2

description:
P2 AKT
Gene expression data from Patient P AKT Day 10.


title: Patient C VEH Day 10 repeat 2

description:
C2 VEH
Gene expression data from Patient C VEH Day 10.


title: Patient M AKT Day 10 repeat 1

description:
M1 AKT
Gene expression data from Patient M AKT Day 10.


title: Patient M AKT Day 10 repeat 4

description:
M4 AKT
Gene expression data from Patient M AKT Day 10.


title: SpleenTRNurr77High_rep1_227


title: Patient C AKT Day 10 repeat 2

description:
C2 AKT
Gene expression data from Patient C AKT Day 10.


title: SpleenTRNurr77High_rep1_226


title: SpleenTRNurr77High_rep1_225


title: Patient C AKT Day 10 repeat 3

description:
C3 AKT
Gene expression data from Patient C AKT Day 10.


title: SpleenTRNurr77High_rep1_224


title: Patient C AKT Day 10 repeat 1

description:
C1 AKT
Gene expression data from Patient C AKT Day 10.


title: Patient P time 0 repeat 2

description:
P2
Gene expression data from Patient P time 0.

source_name: PBMCs, Time 0, Patient P

value: Time 0


title: SpleenTRNurr77High_rep1_223


title: SpleenTRNurr77High_rep1_222


title: Patient P time 0 repeat 3

description:
P3
Gene expression data from Patient P time 0.


title: Patient P time 0 repeat 1

description:
P1
Gene expression data from Patient P time 0.


title: SpleenTRNurr77High_rep1_220


title: SpleenTRNurr77High_rep1_219


title: SpleenTRNurr77High_rep1_217


title: SpleenTRNurr77High_rep1_218


title: SpleenTRNurr77High_rep1_214


title: SpleenTRNurr77High_rep1_213


title: SpleenTRNurr77High_rep1_212


title: SpleenTRNurr77High_rep1_215


title: SpleenTRNurr77High_rep1_216


title: SpleenTRNurr77High_rep1_211


title: SpleenTRNurr77High_rep1_221


title: SpleenTRNurr77High_rep1_210


title: Patient M time 0 repeat 2

description:
M2
Gene expression data from Patient M time 0.

source_name: PBMCs, Time 0, Patient M


title: Patient C time 0 repeat 2

description:
C2
Gene expression data from Patient C time 0.

source_name: PBMCs, Time 0, Patient C


title: HSC-3 MMP-8 migrating

city: Oulu

first: Pirjo

last: Åström

address: Aapistie 5A

country: Finland

institute: University of Oulu

description: HSC-3 cell line is an aggressive human oral tongue squamous cell carcinoma cell line labeled with RFP by stable transduction with commercial lentiviral particles containing human MMP8 gene sequence.

hyb_protocol: The cRNA was fragmented to 35 to 200 nt prior to hybridization to Affymetrix Human Genome U133 Plus 2.0 arrays containing approximately 55 000 human transcripts. The array was washed and stained with streptavidin-phycoerythrin (Molecular Probes, Eugene, OR). Finally, biotinylated anti-streptavidin (Vector Laboratories Inc., Burlingame, CA) was used to amplify the staining signal and a second staining was performed with streptavidin-phycoerythrin.

scan_protocol: The arrays were scanned on a GeneChip Scanner 3000.

data_processing: The expression data was analyzed using dChip software.

label_protocol: Three RNA samples were pooled; the pools contained an equal amount of RNA from each sample. In total, 1 µg of total RNA was used as a template to synthesize biotinylated cRNA by means of the GeneChip 3’IVT Express kit (Affymetrix, Santa Clara, CA) according to the manufacturer’s instructions.

growth_protocol: HSC-3 (Japan Health Sciences Foundation, Japan) cells were cultured in a 1:1 DMEM/F-12 medium supplemented with 100 U/ml penicillin, 100 µg/ml streptomycin, 250 ng/ml fungizone, 50 µg/ml ascorbic acid and 0.4 µg/ml hydrocortisone and 10% heat inactivated fetal bovine serum. HSC-3 cells transduced with commercial lentiviral particles containing a non-coding control sequence or MMP8 and selected with puromycin.

extract_protocol: The cells were harvested for RNA extraction using a Qiagen RNA kit (Hilden, Germany).

treatment_protocol: The cells were seeded into uncoated 6-well plates (three wells each). The next day the cells were harvested for RNA extraction.

tag: line

value: oral tongue squamous cell carcinoma line HSC-3

tag: phenotype

value: migrating

tag: genotype/variation

value: MMP-8 overexpression


title: HSC-3 control

description: HSC-3 cell line is an aggressive human oral tongue squamous cell carcinoma cell line transducted with commercial lentiviral particles containing a non-coding control sequence.

value: stationary

value: control


title: HSC-3 control migrating


title: Patient M time 0 repeat 3

description:
M3
Gene expression data from Patient M time 0.