title: CJ7.EB14.WT.1

description: the cell line is from Stuart Orkin Lab


title: EmbryonicStemCell

status: Public on Feb 14 2018

type: SRA

city: hangzhou

first: guoji

last: guo

state: zhejiang

address: yuhangtang road 388

department: Center for Stem Cell and Regenerative Medicine

country: China

institute: Zhejiang University School of Medicine

description: the cell line is from George Daley Lab

library_source: transcriptomic

data_processing:
Base-calls were performed with Illumina bcl2fastq
Sequenced reads were trimmed for adaptor sequence.Then reads in bam files were tagged with the cell and molecular (UMI) barcode sequences using Drop-seq_tools-1.12.The cell barcodes are tagged as XC in the bam file and the UMI is tagged as XM in the bam file. The reads quality under 10 were removed.
ScRNA-seq reads were aligned to the Mus_musculus.GRCm38.88 genome assembly using STAR version (2.5.2a)  with default configurations.
Next, merge the STAR alignment tagged bam SAM to recover cell /molecular barcodes and the reads are annotated with exon tags.
Last, we demultiplexed all cell barcodes taken into consideration for the analysis from the exon-tagged bam files to get a digital expression matrix based on UMI counts.
Genome_build: Mus_musculus.GRCm38.88 genome
Supplementary_files_format_and_content: tab-delimited text files of digital expression matrix (dge) based on raw UMI counts ,columns are cells, and rows are genes.

molecule: total RNA

organism: Mus musculus

extract_protocol:
Tissue dissociation and  single-cell lysate
Library preparations were performed with the Microwell-seq protocol

tag: cell line


title: Bone.Marrow.Mesenchyme

tag: tissue

tag: age

value: 6-10 week-old

tag: gender

value: female


title: BoneMarrowcKit3

source_name: BoneMarrowcKit

value: BoneMarrowcKit


title: BoneMarrowcKit2


title: BoneMarrowcKit1


title: MammaryGland.Involution2

description: Microwell-seq analysis

tag: strain

value: post natal 6 - 8 week


title: Brain2

source_name: Brain

value: Brain


title: BoneMarrow1

source_name: BoneMarrow

value: BoneMarrow


title: CJ7.EB14.Ezh2.1

description: the cell line is from Stuart Orkin Lab

source_name: EB


title: BoneMarrow2


title: Prostate1

description: Microwell-seq analysis

source_name: Prostate

value: Prostate


title: BoneMarrow3


title: Prostate2

description: Microwell-seq analysis


title: Bladder

description: Microwell-seq analysis

source_name: Bladder

value: Bladder


title: MammaryGland.Involution1

description: Microwell-seq analysis


title: FetalBrain

source_name: FetalBrain

value: FetalBrain

value: E14.5 embryos


title: BLADDER tumor [Sample 149]

city: Gaithersburg

first: Yinong

last: Sebastian

address: One MedImmune Way

country: USA

institute: Medimmune

description:
Sample 149
processed data file: CP1108_UBC_21gene_expression.txt

data_processing:
Basecalls performed using bcl2fastq (v2-2.19).
Reads were aligned to hg19 reference using STAR (v2.5.2).
Transcripts were quantified using RSEM (v1.2.30) and the GRCh37.68 gtf annotation file.
Genome_build: hg19 (GRCh37)
Supplementary_files_format_and_content: *_21gene_expression.txt: Normalized expression values provided in a log2 scale.

organism: Homo sapiens

source_name: Bladder tumor

extract_protocol:
RNA from tumor samples was isolated using Quick-RNA Microprep kit (Cat#R1050) from Zymo Research.
Libraries were prepared for sequencing using standard Illumina protocols. RNA input for library prep was 150ng. Followed Illumina TruSeq Stranded Total RNA with ribo-depletion (gold) protocol for library prep. Cat# 20020599

value: Locally advanced or metastatic urothelial cancer (UC) tumor

tag: sample type

value: needle biopsy


title: BLADDER tumor [Sample 153]

description:
Sample 153
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 147]

description:
Sample 147
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 143]

description:
Sample 143
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 144]

description:
Sample 144
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 146]

description:
Sample 146
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 158]

description:
Sample 158
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 152]

description:
Sample 152
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 145]

description:
Sample 145
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 159]

description:
Sample 159
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 148]

description:
Sample 148
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 156]

description:
Sample 156
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 151]

description:
Sample 151
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 157]

description:
Sample 157
processed data file: CP1108_UBC_21gene_expression.txt


title: Pim2-sh RNA rep1

type: RNA

city: Paris

first: Franck

last: Letourneur

address: 22 rue mechain

country: France

institute: INSERM

hyb_protocol: biotynilated single stranded sense cDNA is then hybridized to GeneChip® Human Gene2.0 (Affymetrix) at 45°C for 17 hours

scan_protocol: After overnight hybridization, chips are washed on the fluidic station FS450 following specific protocols (Affymetrix) and scanned using the GCS3000 7G

data_processing: Data were normalized using RMA and statistical analysis was realized on Partek Genomics Suite. CDF file V16 was used for annotation.

label: biotin

source_name: Pim2-sh RNA

label_protocol: 50 ng of total RNA is reverse transcribed following the protocol of the Ovation PicoSL WTA System V2 (Nugen). Briefly, the resulting double stranded cDNA is used for amplification based on SPIA technology. Amplified sense targets DNA are fragmented and biotin labelled using Encore Biotin Module kit (Nugen).

growth_protocol: MOLM-14 cells transduced by doxycycline-inducible scrambled or anti-Pim2 shRNA were cultured in 10% fetal bovine serum (FBS, Gibco, # 10270-106, ThermoFischer)-supplemented alpha modified Eagle's medium (MEM), with 4mM glutamine and penicillin/streptomycin.

extract_protocol: Total RNA was extracted with DNase I digestion (RNeasy Mini Kit used with the Bio-robot QIAcube).

treatment_protocol: MOLM-14 cells transduced by doxycycline-inducible scrambled or anti-Pim2 shRNA were cultured 72h in the presence of 200 ng/ml doxycycline.

value: MOLM-14 AML cell line

tag: genotype/variation

value: Pim2 knockdown


title: BLADDER tumor [Sample 155]

description:
Sample 155
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 154]

description:
Sample 154
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 139]

description:
Sample 139
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 142]

description:
Sample 142
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 141]

description:
Sample 141
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 137]

description:
Sample 137
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 135]

description:
Sample 135
processed data file: CP1108_UBC_21gene_expression.txt


title: scrambled-sh control rep1

source_name: scrambled-sh control

value: control


title: BLADDER tumor [Sample 136]

description:
Sample 136
processed data file: CP1108_UBC_21gene_expression.txt


title: scrambled-sh control rep2


title: BLADDER tumor [Sample 138]

description:
Sample 138
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 134]

description:
Sample 134
processed data file: CP1108_UBC_21gene_expression.txt


title: Pim2-sh RNA rep2


title: BLADDER tumor [Sample 150]

description:
Sample 150
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 140]

description:
Sample 140
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 128]

description:
Sample 128
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 129]

description:
Sample 129
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 126]

description:
Sample 126
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 123]

description:
Sample 123
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 122]

description:
Sample 122
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 127]

description:
Sample 127
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 132]

description:
Sample 132
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 113]

description:
Sample 113
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 118]

description:
Sample 118
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 131]

description:
Sample 131
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 114]

description:
Sample 114
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 117]

description:
Sample 117
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 110]

description:
Sample 110
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 130]

description:
Sample 130
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 115]

description:
Sample 115
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 112]

description:
Sample 112
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 107]

description:
Sample 107
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 125]

description:
Sample 125
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 133]

description:
Sample 133
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 119]

description:
Sample 119
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 120]

description:
Sample 120
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 124]

description:
Sample 124
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 105]

description:
Sample 105
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 116]

description:
Sample 116
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 111]

description:
Sample 111
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 109]

description:
Sample 109
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 108]

description:
Sample 108
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 104]

description:
Sample 104
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 121]

description:
Sample 121
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 106]

description:
Sample 106
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 102]

description:
Sample 102
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 103]

description:
Sample 103
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 101]

description:
Sample 101
processed data file: CP1108_UBC_21gene_expression.txt


title: BLADDER tumor [Sample 100]

description:
Sample 100
processed data file: CP1108_UBC_21gene_expression.txt


title: NSCLC tumor [Sample 97]

description:
Sample 97
processed data file: CP1108_NSCLC_21gene_expression.txt

source_name: NSCLC tumor

value: Advanced-stage non-small-cell lung carcinoma (NSCLC) tumor


title: NSCLC tumor [Sample 96]

description:
Sample 96
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 93]

description:
Sample 93
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 90]

description:
Sample 90
processed data file: CP1108_NSCLC_21gene_expression.txt


title: BLADDER tumor [Sample 99]

description:
Sample 99
processed data file: CP1108_UBC_21gene_expression.txt


title: NSCLC tumor [Sample 83]

description:
Sample 83
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 84]

description:
Sample 84
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 89]

description:
Sample 89
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 92]

description:
Sample 92
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 87]

description:
Sample 87
processed data file: CP1108_NSCLC_21gene_expression.txt


title: BLADDER tumor [Sample 98]

description:
Sample 98
processed data file: CP1108_UBC_21gene_expression.txt


title: NSCLC tumor [Sample 95]

description:
Sample 95
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 86]

description:
Sample 86
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 91]

description:
Sample 91
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 78]

description:
Sample 78
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 81]

description:
Sample 81
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 79]

description:
Sample 79
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 85]

description:
Sample 85
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 80]

description:
Sample 80
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 76]

description:
Sample 76
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 77]

description:
Sample 77
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 82]

description:
Sample 82
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 74]

description:
Sample 74
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 75]

description:
Sample 75
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 94]

description:
Sample 94
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 71]

description:
Sample 71
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 72]

description:
Sample 72
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 73]

description:
Sample 73
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 88]

description:
Sample 88
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 70]

description:
Sample 70
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 69]

description:
Sample 69
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 68]

description:
Sample 68
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 65]

description:
Sample 65
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 55]

description:
Sample 55
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 61]

description:
Sample 61
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 63]

description:
Sample 63
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 59]

description:
Sample 59
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 58]

description:
Sample 58
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 64]

description:
Sample 64
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 62]

description:
Sample 62
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 57]

description:
Sample 57
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 60]

description:
Sample 60
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 54]

description:
Sample 54
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 53]

description:
Sample 53
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 49]

description:
Sample 49
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 52]

description:
Sample 52
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 51]

description:
Sample 51
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 50]

description:
Sample 50
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 47]

description:
Sample 47
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 48]

description:
Sample 48
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 67]

description:
Sample 67
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 44]

description:
Sample 44
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 46]

description:
Sample 46
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 45]

description:
Sample 45
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 41]

description:
Sample 41
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 66]

description:
Sample 66
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 56]

description:
Sample 56
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 37]

description:
Sample 37
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 29]

description:
Sample 29
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 36]

description:
Sample 36
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 32]

description:
Sample 32
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 28]

description:
Sample 28
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 33]

description:
Sample 33
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 31]

description:
Sample 31
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 27]

description:
Sample 27
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 26]

description:
Sample 26
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 35]

description:
Sample 35
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 24]

description:
Sample 24
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 23]

description:
Sample 23
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 39]

description:
Sample 39
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 22]

description:
Sample 22
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 40]

description:
Sample 40
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 42]

description:
Sample 42
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 21]

description:
Sample 21
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 43]

description:
Sample 43
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 20]

description:
Sample 20
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 16]

description:
Sample 16
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 19]

description:
Sample 19
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 38]

description:
Sample 38
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 15]

description:
Sample 15
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 25]

description:
Sample 25
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 17]

description:
Sample 17
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 13]

description:
Sample 13
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 14]

description:
Sample 14
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 30]

description:
Sample 30
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 34]

description:
Sample 34
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 12]

description:
Sample 12
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 10]

description:
Sample 10
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 8]

description:
Sample 8
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 5]

description:
Sample 5
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 4]

description:
Sample 4
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 6]

description:
Sample 6
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 1]

description:
Sample 1
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 3]

description:
Sample 3
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 2]

description:
Sample 2
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 18]

description:
Sample 18
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 7]

description:
Sample 7
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NSCLC tumor [Sample 9]

description:
Sample 9
processed data file: CP1108_NSCLC_21gene_expression.txt


title: OV_3_1

city: Tucson

first: Dominic

last: LaRoche

state: AZ

address: 3430 E. Global Loop

institute: HTG Molecular Diagnostics

data_processing:
Illumina CASAVA is used for base calling and generation of FASQ files
HTG EdgeSeq Parser software (version 10196100F) is used for alignment to a priori defined target sequences.  Requirements include %Q30 >= 85%, %PF >= 75%, and 180 < CD < 290
Median normalization (Dillies, 2013) was used to transform and standardize data
Supplementary_files_format_and_content: counts

source_name: Otic Vesicle

extract_protocol:
HTG EdgeSeq WT-miRNA Assay (Tucson, Arizona)
Samples were processed by adding HTG Lysis Buffer to lyse and/or permeabilize the RNA available to subsequently bind to corresponding target-specific Nuclease Protection Probes (NPPs). The lysed samples was transferred to a 96-well micro-titer plate. Target capture was done by adding NPPs to the lysed samples to hybridize to the target mRNA. Then S1 nuclease was added to digest non-hybridized miRNA and excess NPPs, leaving target mRNA/NPP duplexes. S1 digestion was terminated by heat denaturation of S1 enzyme. The processed samples were used as template to set up PCR reactions with primer tags. These tags share common sequences that are complementary to 5’-end and 3’-end “wing” sequences of the probes and common adaptors required for cluster generation for deep sequencing. The library was prepared using HTG EdgeSeq PCR processing. Following library preparation, it was quantified using HTG EdgeSeq KAPA Library Quantification for Illumina Sequencing.
HTG EdgeSeq KAPA Library Quantification/Calculator

tag: time-point

tag: replicate


title: CVG_2_1

source_name: Vestibulochlear Complex


title: CVG_2_2


title: OV_3_2


title: OV_2_1


title: CVG_3_1


title: OV_2_2


title: CVG_3_2


title: CVG_1_2


title: CVG_1_1


title: OV_1_2


title: NC_3_1

source_name: Neural Crest


title: NC_2_2


title: NC_3_2


title: NC_1_2


title: NC_2_1


title: NSCLC tumor [Sample 11]

description:
Sample 11
processed data file: CP1108_NSCLC_21gene_expression.txt


title: NC_1_1


title: CVG_3_3


title: NC_3_3


title: CVG_2_3


title: OV_2_3


title: OV_3_3


title: OV_1_3


title: OV_1_1


title: NC_2_3


title: CVG_1_3


title: NC_1_3


title: Zfp36l1 shRNA Adenovirus infected primary mouse articular chondrocytes, biological rep 1

city: Gwangju

first: Yoonkyung

last: Won

address: 123 Cheomdangwagiro

country: South Korea

institute: Gwangju Institute of Science and Technology

description:
Gene expression data from Zfp36l1 shRNA Adenovirus infected primary mouse articular chondrocytes
Zfp36l1 shRNA Adenovirus infected_biological rep 1_(MoGene-2_0-st).rma-gene-full-Signal

hyb_protocol: Approximately 5.5 μg of labeled DNA target was hybridized to the Affymetrix GeneChip Array at 45°C for 16hour

scan_protocol: Hybridized arrays were washed and stained on a GeneChip Fluidics Station 450 and scanned on a GCS3000 Scanner (Affymetrix).

data_processing:
Array data export processing and analysis was performed using Affymetrix® GeneChip Command Console® Software (AGCC)
Probe signals in the raw data were normalized by the RMA (Robust Multi-array Average)

label_protocol: The sense cDNA was then fragmented and biotin-labeled with TdT (terminal deoxynucleotidyl transferase) using the GeneChip WT Terminal labeling kit

growth_protocol: Cultured were maintained at 37 °C in an humidified atmosphere containing 5% CO2 as a monolayer in Dulbecco’s modified Eagle’s medium (DMEM; Gibco) supplemented with 10% fetal bovine serum and antibiotics (penicillin G and streptomycin). 37 °C, 5% CO2

extract_protocol: Total RNA was extracted from mouse articular chondrocytes using a Purelink RNA mini kit (Ambion). The concentration, purity, and integrity of the extracted RNA were determined by spectrophotometry.

treatment_protocol: Primary mouse articular chondrocytes were infected with recombinant adenovirus expressing mouse ZFP36 Ring Finger Protein Like 1(Zfp36l1) or recombinant adenovirus expressing mouse small hairpin RNA of Zfp36l1. The cells were harvested for total RNA extraction at 36 h post treatment.

tag: cell type

value: Primary mouse articular chondrocytes

tag: infected with

value: Zfp36l1 shRNA Adenovirus


title: Zfp36l1 shRNA Adenovirus infected primary mouse articular chondrocytes, biological rep 2

description:
Gene expression data from Zfp36l1 shRNA Adenovirus infected primary mouse articular chondrocytes
Zfp36l1 shRNA Adenovirus infected_biological rep 2_(MoGene-2_0-st).rma-gene-full-Signal


title: Chd7f/f, Mx1-Cre, Cbfb+/56M_1

city: BETHESDA

first: abdel

middle: G

last: Elkahloun

address: 50, SOUTH DRIVE

institute: NHGRI-NIH

hyb_protocol: The hybridization cocktail containing the fragmented and labeled cDNAs were hybridized to Affymetrix mouse GeneChip 2.0 ST microarrays.  The microarrays were washed and stained in an Affymetrix Fluidics Station using standard Affymetrix protocols.

scan_protocol: Genechips were scanned using an Affymetrix Gene Chip Scanner 3000.

data_processing: Gene expression intensities were calculated using GeneChip Command Console Software (AGCC). .cel files generated by the Affymetrix AGCC program were imported in the Affymetrix Expression Console software and RMA (Robust Multichip Analysis) normalization was performed to generate the .chp files. The .chp files were normalized, log2 transformed, and summarized.

label: Biotin

source_name: c-Kit+ leukemic cells from spleen

label_protocol: Labeling was performed on a sample-by-sample basis according to manufacturer’s guidelines for use with the Affymetrix Mouse HuGene-2_0-st-v1 GeneChips (Affymetrix, Inc).  DNase treatment was included as part of isolation to remove possible contaminating DNA.  Bioanalyzer nanochip (Agilent, Inc) and NanoDrop (ThermoFisher, Inc) were used to validate and quantitate the RNA prior to cRNA synthesis and labeling.  300 ng of total RNA was used for labeling with theAmbion WT Expression Kit (cat#4411974)  and Affymetrix kit (Cat # 900652).After hybridization,  the probe arrays were stained with streptavidin-phycoerythrin solution (Molecular Probes) and enhanced using an antibody solution containing 0.5 mg/mL of biotinylated anti-streptavidin (Vector Laboratories).

extract_protocol: RNA was extracted by suing AllPrep® DNA/RNA/Protein Mini kit from QIAGEN according to the manufacturer’s instruction

treatment_protocol: Mx1-Cre, Cbfb+/56M and Chd7f/f, Mx1-Cre, Cbfb+/56M mice were treated with ENU and pIpC, after these mice developed leukemia, c-Kit+ cells were sorted out from end stage mice spleen cels,

value: spleen

value: c-Kit+ leukemic cells

tag: genotype

value: Chd7f/f, Mx1-Cre, Cbfb(+/56M)


title: Chd7f/f, Mx1-Cre, Cbfb+/56M_2


title: Chd7f/f, Mx1-Cre, Cbfb+/56M_3


title: BEZ235_replicate 2

first: Pierre

last: de la Grange

address: 29, rue du Faubourg Saint-Jacques

department: Paris Biotech Santé

institute: GenoSplice technology

hyb_protocol: Samples were hybridized using Affymetrix hybridization kit materials • Heat cocktails at 99° for 5 minutes, then 42° for 5 minutes centrifuge at max speed for 1 minute (N.B. this deviates from Affy SOP). • Transfer 200μl of hyb solution to each array, then tape holes and parafilm. • Hybridize 16 hours at 45° at 60rpm• Fluidics washing is FS450_0001

scan_protocol: Affymetrix Gene ChIP Scanner 3000 7G

data_processing: Data were processed by GenoSplice technology using RMA GC-BG analysis

source_name: TC71 Ewing Sarcoma cell line

label_protocol: Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)

growth_protocol: Human TC71 cells were maintained in Iscove’s modified Dulbecco’s medium (IMDM, GIBCO), supplemented with 10% fetal bovine serum, penicillin and streptomycin (Gibco), and maintained at 37°C in humidified 5% CO2 atmosphere.

extract_protocol: RNA from three biological replicates of either control or BEZ235 treated TC-71 cells swas purified using RNeasy Mini kit (Qiagen) and digested with DNase RNase free (Qiagen).

treatment_protocol: The BEZ235 inhibitor was purchased from EMD Chemical Inc./Calbiochem and dissolved in dimethyl sulfoxide. TC71 cells were treated either with BEZ235 or DMSO for 16 hours. After treatmnet cells were washed twice with PBS and collected in RLT buffer (Qiagen).

tag: agent


title: Mx1-Cre, Cbfb+/56M_3

value: Mx1-Cre, Cbfb(+/56M)


title: Mx1-Cre, Cbfb+/56M_1


title: DMSO_replicate 3

value: DMSO


title: DHA 4 hr treatment (dha426)

city: Memphis

first: David

last: Finkelstein

state: TN

address: 332 N. Lauderdale St.

department: Computational Biology

institute: St Jude Children's Research Hospital

hyb_protocol: Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mogene ST 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.

scan_protocol: GeneChips were scanned using the GeneChip Scanner 3000 7G

data_processing: The data were analyzed with Partek Genomics Suite version 6.6 using the RMA method.

source_name: mouse BCR-ABL+ B-ALL cells

label_protocol: Biotinylated cRNA were prepared according to the standard Affymetrix  protocol.  Biotin (Affyexpress).

growth_protocol: Mouse p185+ or p185T315I+Arf-/- or p53-/- B-ALL were grown in RPMI (Life Technologies, CA) with 10% fetal bovine serum, 55 µM 2-mercaptoethanol, 2 mM glutamine, penicillin, and streptomycin.

extract_protocol: Five million mouse BCR-ABL+ B-ALL cells were treated with 312 nM DHA or with vehicle (DMSO) for 2 and 4 hours. After the treatment, cells were harvested and washed with PBS and total RNA was isolated using PureLink RNA Mini Kit from Ambion (Life Technologies, CA). Briefly, cells were lysed in lysis buffer containing 1% 2- Mercaptoethanol (2-ME) and homogenized by passing through a 21-gauze needle. Homogenates were transferred to spin cartridge and centrifuged at 12,000 g for 15 seconds, washed with wash buffer I and twice with wash buffer II (with ethanol). Spin cartridges were further centrifuged at 12,000 g for 15 seconds to dry the membrane with bound RNA. 30-60 µL RNase-free water was added to the center of the cartridge and elutes were collected in an Eppendorf and stored at -800 C. RNA concentrations were determined by nanodrop and ratio of absorbance at 260 nm/280 nm of 2 or over were sent for microarray analysis

treatment_protocol: Five million mouse BCR-ABL+ B-ALL cells were treated with 312 nM DHA or with vehicle (DMSO) for  4 hours

value: DHA

value: BCR-ABL+ B-ALL cells


title: Null Control Adenovirus infected primary mouse articular chondrocytes, biological rep 3

description:
Gene expression data from Null Control Adenovirus infected primary mouse articular chondrocytes
Null Control Adenovirus_biological rep 3_C3_(MoGene-2_0-st).rma-gene-full-Signal

value: Null Control Adenovirus


title: DMSO_replicate 2


title: Null Control Adenovirus infected primary mouse articular chondrocytes, biological rep 1

description:
Gene expression data from Null Control Adenovirus infected primary mouse articular chondrocytes
Null Control Adenovirus_biological rep 1_(MoGene-2_0-st).rma-gene-full-Signal


title: Null Control Adenovirus infected primary mouse articular chondrocytes, biological rep 2

description:
Gene expression data from Null Control Adenovirus infected primary mouse articular chondrocytes
Null Control Adenovirus_biological rep 2_(MoGene-2_0-st).rma-gene-full-Signal


title: DHA 4 hr treatment (dha416)


title: DMSO control  (con226)


title: DMSO_replicate 1


title: DHA 4 hr treatment (dha246)


title: DMSO control  (con216)


title: Zfp36l1 Adenovirus infected primary mouse articular chondrocytes, biological rep 2

description:
Gene expression data from Zfp36l1 Adenovirus infected primary mouse articular chondrocytes
Zfp36l1 Adenovirus infected_biological rep 2_(MoGene-2_0-st).rma-gene-full-Signal

value: Zfp36l1 Adenovirus


title: Zfp36l1 Adenovirus infected primary mouse articular chondrocytes, biological rep 3

description:
Gene expression data from Zfp36l1 Adenovirus infected primary mouse articular chondrocytes
Zfp36l1 Adenovirus infected_biological rep 3_(MoGene-2_0-st).rma-gene-full-Signal


title: Zfp36l1 Adenovirus infected primary mouse articular chondrocytes, biological rep 1

description:
Gene expression data from Zfp36l1 Adenovirus infected primary mouse articular chondrocytes
Zfp36l1 Adenovirus infected_biological rep 1_(MoGene-2_0-st).rma-gene-full-Signal


title: DMSO control  (con416)


title: Mx1-Cre, Cbfb+/56M_2


title: BEZ235_replicate 1


title: JAG028: C2C12 + 12.5 uM compound # 181

hyb_protocol: Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.

data_processing: The data were analyzed with Partek Genomics Suite version 6.5 using the RMA method.

source_name: mouse myoblast cell line C2C12

label_protocol: Biotinylated cRNA were prepared according to the standard Affymetrix one-Cycle protocol).  Some samples were amplified and labeled using the Affyexpress method. Three samples were amplified by both method to serve as basis for batch correction. Biotinylated cRNA were prepared according to the standard Affymetrix one-Cycle protocol).  Some samples were amplified and labeled using the Affyexpress method. Three samples were amplified by both method to serve as basis for batch correction

growth_protocol: The mouse myoblast cell line C2C12 was grown in DMEM supplemented with 5% FBS, 2 mM glutamine, 500 units/ml penicillin and 500 µg/ml streptomycin and grown at 37°C and 8% CO2.  To avoid depletion of the myoblastic population, C2C12 cells were not allowed to grow to confluence and were passaged at a density less than 70%.

extract_protocol: Total RNA was extracted with TRIzol reagent (Ambion by Life Technologies).

treatment_protocol: 20 Briefly, 2x104 C2C12 cells were plated in 24-well plates and allowed to grow O/N at 37°C. The next morning medium was replaced with fresh medium containing 5% FBS and individual wells were treated with MeOH, 6.25 μM or 25 μM of each compound or recombinant BMP-4 (10, 300 ng/mL).

value: myoblast

tag: treatment

value: 12.5 uM compound # 181


title: JAG027: C2C12 + 6.25uM compound # 181

value: 6.25uM compound # 181


title: Zfp36l1 shRNA Adenovirus infected primary mouse articular chondrocytes, biological rep 3

description:
Gene expression data from Zfp36l1 shRNA Adenovirus infected primary mouse articular chondrocytes
Zfp36l1 shRNA Adenovirus infected_biological rep 3_(MoGene-2_0-st).rma-gene-full-Signal


title: JAG029: C2C12 + 25uM compound # 181

value: 25uM compound # 181


title: JAG026: C2C12 + 25uM compound # 178

value: 25uM compound # 178


title: BEZ235_replicate 3


title: JAG024: C2C12 + 6.25uM compound # 178

value: 6.25uM compound # 178


title: JAG025: C2C12 + 12.5 uM compound # 178

value: 12.5 uM compound # 178


title: JAG018: C2C12 untreated

value: untreated


title: JAG020: C2C12 + methanol

value: methanol


title: JAG013: C2C12 + BMP4 100ng/ml

value: BMP4 100ng/ml


title: JAG015: C2C12 + 6.25 uM 942

label: Biotin (Affyexpress)


title: JAG010: C2C12 untreated


title: JAG019: C2C12 + methanol


title: JAG023: C2C12 + BMP4 300ng/ml

value: BMP4 300ng/ml


title: JAG016: C2C12 + 12.5 uM 942


title: JAG022: C2C12 + BMP4 100ng/ml


title: JAG021: C2C12 + BMP4 10ng/ml

value: BMP4 10ng/ml


title: JAG017: C2C12 + 25.0 uM 942


title: JAG014: C2C12 + BMP4 300ng/ml


title: JAG011: C2C12 + methanol


title: Cre^(ER)/Rptor^(fl/+) pro-B cells treated with IL-7 replicate 3

first: Geoffrey

last: Neale

address: 262 Danny Thomas Place

department: Hartwell Center

institute: St Jude Childrens Research Hospital

hyb_protocol: Six micrograms of biotinylated cDNA was fragmented and then hybridized for 16 hr at 45C on an Affymetrix Mouse Gene 2.0 ST GeneChip. Following hybridization, GeneChips were washed and stained in the Affymetrix Fluidics Station 450.

scan_protocol: GeneChips were scanned using the AffymetrixGeneChip Scanner 3000 7G.

data_processing: Probe signals were quantile normalized and summarized by the RMA algorithm using Partek Genomics Suite software v6.6

source_name: IL-7 treated pro-B cells from Cre^(ER)/Rptor^(fl/+) mouse

label_protocol: One to five nanograms of total RNA were converted into biotinylated cDNA using the NuGEN WTA Pico v2 protocol and the NuGEN Encore Biotin module

growth_protocol: Lymphocytes were isolated from bone marrow of mice, and pro-B cells (B220+CD43+IgM-) were sorted on a MoFlow (Beckman-Coulter) or Reflection (i-Cyt).

extract_protocol: RNA was extracted using Qiagen's RNAeasy kit, with on-column DNase digestion included.

treatment_protocol: Pro-B cells from tamoxifen-treated Cre^(ER)/Rptor^(fl/+) and Cre^(ER)/Rptor^(fl/fl) mice were cultured in the presence or absence of 25 ng/ml IL-7 for 24h.

tag: cell population

value: B220+CD43+IgM- pro-B

value: C57BL/6 Cre^(ER)/Rptor^(fl/+)


title: Cre^(ER)/Rptor^(fl/+) pro-B cells treated with IL-7 replicate 2


title: Cre^(ER)/Rptor^(fl/fl) pro-B cells treated with IL-7 replicate 3

source_name: IL-7 treated pro-B cells from Cre^(ER)/Rptor^(fl/fl) mouse

value: C57BL/6 Cre^(ER)/Rptor^(fl/fl)


title: Cre^(ER)/Rptor^(fl/fl) pro-B cells treated with IL-7 replicate 2


title: Cre^(ER)/Rptor^(fl/fl) pro-B cells treated with IL-7 replicate 1


title: Cre^(ER)/Rptor^(fl/fl) pro-B cells untreated replicate 1

source_name: Untreated pro-B cells from Cre^(ER)/Rptor^(fl/fl) mouse


title: Cre^(ER)/Rptor^(fl/+) pro-B cells treated with IL-7 replicate 1


title: Cre^(ER)/Rptor^(fl/+) pro-B cells untreated replicate 3

source_name: Untreated pro-B cells from Cre^(ER)/Rptor^(fl/+) mouse


title: Cre^(ER)/Rptor^(fl/+) pro-B cells untreated replicate 2


title: Cre^(ER)/Rptor^(fl/fl) pro-B cells untreated replicate 3


title: Cre^(ER)/Rptor^(fl/fl) pro-B cells untreated replicate 2


title: Cre^(ER)/Rptor^(fl/+) pro-B cells untreated replicate 1


title: JAG012: C2C12 + BMP4 10ng/ml


title: LPA DL Le 2933

city: Dummerstorf

first: Siriluck

last: Ponsuksili

address: Wilhelm-stahl-allee 2

country: Germany

institute: FBN

description: microRNA expression data from liver

hyb_protocol: Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 42C on Affymetrix GeneChip miRNA arrays. GeneChips were washed and processed to detect biotin-containing transcripts by Streptavidin-PE (Phycoerythrin) conjugate.

scan_protocol: Scanning using the GeneChip scanner 3000 were performed according to Affymetrix protocols

data_processing: Data were analysed with Expression Console and quantify by med polish

organism: Sus scrofa

source_name: Liver

label_protocol: Biotinylated cRNA were prepared from miRNA according to FlashTag Biotin RNA Labeling Kit for Affymetrix GeneChip miRNA arrays (Genisphere, Hatfield, PA, USA) according to the manufacturer’s recommendations Affymetrix GeneChip miRNA Array protocols (Affymetrix, Santa Clara, CA, USA)

extract_protocol: Total RNA was isolated using Qiagen miReasy Mini kit TRI Reagent (Qiagen, Hilden, Germany) according to the manufacturer’s protocol for small-RNA. Then small RNA was cleaned up using the Rneasy MinElute Clean up kit  (Qiagen, Hilden, Germany).

value: adult

tag: body weight

tag: meat quality grade


title: LPA DL Le 2932

description: microRNA expression data from liver


title: LPA DL Le 2927

description: microRNA expression data from liver


title: LPA DL Le 2929

description: microRNA expression data from liver


title: LPA DL Le 2928

description: microRNA expression data from liver


title: LPA DL Le 2931

description: microRNA expression data from liver


title: LPA DL Le 2926

description: microRNA expression data from liver


title: LPA DL Le 2923

description: microRNA expression data from liver


title: LPA DL Le 2922

description: microRNA expression data from liver


title: LPA DL Le 2925

description: microRNA expression data from liver


title: LPA DL Le 2919

description: microRNA expression data from liver


title: LPA DL Le 2916

description: microRNA expression data from liver


title: LPA DL Le 2921

description: microRNA expression data from liver


title: LPA DL Le 2917

description: microRNA expression data from liver


title: LPA DL Le 2908

description: microRNA expression data from liver


title: LPA DL Le 2913

description: microRNA expression data from liver


title: LPA DL Le 2906

description: microRNA expression data from liver


title: LPA DL Le 2910

description: microRNA expression data from liver


title: LPA DL Le 2907

description: microRNA expression data from liver


title: LPA DL Le 2918

description: microRNA expression data from liver


title: LPA DL Le 2924

description: microRNA expression data from liver


title: LPA DL Le 2903

description: microRNA expression data from liver


title: LPA DL Le 2909

description: microRNA expression data from liver


title: LPA DL Le 2920

description: microRNA expression data from liver


title: LPA DL Le 2902

description: microRNA expression data from liver


title: LPA DL Le 2899

description: microRNA expression data from liver


title: LPA DL Le 2900

description: microRNA expression data from liver


title: LPA DL Le 2901

description: microRNA expression data from liver


title: LPA DL Le 2905

description: microRNA expression data from liver


title: LPA DL Le 2904

description: microRNA expression data from liver


title: LPA DL Le 2892

description: microRNA expression data from liver


title: LPA DL Le 2891

description: microRNA expression data from liver


title: LPA DL Le 2894

description: microRNA expression data from liver


title: LPA DL Le 2887

description: microRNA expression data from liver


title: LPA DL Le 2896

description: microRNA expression data from liver


title: LPA DL Le 2885

description: microRNA expression data from liver


title: LPA DL Le 2882

description: microRNA expression data from liver


title: LPA DL Le 2883

description: microRNA expression data from liver


title: LPA DL Le 2888

description: microRNA expression data from liver


title: LPA DL Le 2884

description: microRNA expression data from liver


title: LPA DL Le 2878

description: microRNA expression data from liver


title: LPA DL Le 2875

description: microRNA expression data from liver


title: LPA DL Le 2873

description: microRNA expression data from liver


title: LPA DL Le 2874

description: microRNA expression data from liver


title: LPA DL Le 2877

description: microRNA expression data from liver


title: LPA DL Le 2872

description: microRNA expression data from liver


title: LPA DL Le 2897

description: microRNA expression data from liver


title: LPA DL Le 2863

description: microRNA expression data from liver


title: LPA DL Le 2864

description: microRNA expression data from liver


title: LPA DL Le 2861

description: microRNA expression data from liver


title: LPA DL Le 2886

description: microRNA expression data from liver


title: LPA DL Le 2859

description: microRNA expression data from liver


title: LPA DL Le 2847

description: microRNA expression data from liver


title: LPA DL Le 2855

description: microRNA expression data from liver


title: LPA DL Le 2856

description: microRNA expression data from liver


title: LPA DL Le 2846

description: microRNA expression data from liver


title: LPA DL Le 2843

description: microRNA expression data from liver


title: LPA DL Le 2848

description: microRNA expression data from liver


title: LPA DL Le 2862

description: microRNA expression data from liver


title: LPA DL Le 2844

description: microRNA expression data from liver


title: LPA DL Le 2836

description: microRNA expression data from liver


title: LPA DL Le 2835

description: microRNA expression data from liver


title: LPA DL Le 2733

description: microRNA expression data from liver


title: LPA DL Le 2837

description: microRNA expression data from liver


title: LPA DL Le 2840

description: microRNA expression data from liver


title: LPA DL Le 2841

description: microRNA expression data from liver


title: LPA DL Le 2842

description: microRNA expression data from liver


title: LPA DL Le 2838

description: microRNA expression data from liver


title: LPA DL Le 2731

description: microRNA expression data from liver


title: LPA DL Le 2730

description: microRNA expression data from liver


title: LPA DL Le 2729

description: microRNA expression data from liver


title: LPA DL Le 2881

description: microRNA expression data from liver


title: LPA DL Le 2728

description: microRNA expression data from liver


title: LPA DL Le 2722

description: microRNA expression data from liver


title: LPA DL Le 2726

description: microRNA expression data from liver


title: LPA DL Le 2721

description: microRNA expression data from liver


title: LPA DL Le 2719

description: microRNA expression data from liver


title: LPA DL Le 2724

description: microRNA expression data from liver


title: LPA DL Le 2723

description: microRNA expression data from liver


title: LPA DL Le 2711

description: microRNA expression data from liver


title: LPA DL Le 2708

description: microRNA expression data from liver


title: LPA DL Le 2705

description: microRNA expression data from liver


title: LPA DL Le 2718

description: microRNA expression data from liver


title: LPA DL Le 2713

description: microRNA expression data from liver


title: LPA DL Le 2635

description: microRNA expression data from liver


title: LPA DL Le 2717

description: microRNA expression data from liver


title: LPA DL Le 2637

description: microRNA expression data from liver


title: LPA DL Le 2714

description: microRNA expression data from liver


title: LPA DL Le 2620

description: microRNA expression data from liver


title: LPA DL Le 2712

description: microRNA expression data from liver


title: LPA DL Le 2596

description: microRNA expression data from liver


title: LPA DL Le 2594

description: microRNA expression data from liver


title: LPA DL Le 2593

description: microRNA expression data from liver


title: LPA DL Le 2589

description: microRNA expression data from liver


title: LPA DL Le 2587

description: microRNA expression data from liver


title: LPA DL Le 2608

description: microRNA expression data from liver


title: LPA DL Le 2590

description: microRNA expression data from liver


title: LPA DL Le 2583

description: microRNA expression data from liver


title: LPA DL Le 2581

description: microRNA expression data from liver


title: LPA DL Le 2592

description: microRNA expression data from liver


title: LPA DL Le 2579

description: microRNA expression data from liver


title: LPA DL Le 2710

description: microRNA expression data from liver


title: LPA DL Le 2577

description: microRNA expression data from liver


title: LPA DL Le 2502

description: microRNA expression data from liver


title: LPA DL Le 2566

description: microRNA expression data from liver


title: LPA DL Le 2499

description: microRNA expression data from liver


title: LPA DL Le 2490

description: microRNA expression data from liver


title: LPA DL Le 2504

description: microRNA expression data from liver


title: LPA DL Le 2503

description: microRNA expression data from liver


title: LPA DL Le 2493

description: microRNA expression data from liver


title: LPA DL Le 2494

description: microRNA expression data from liver


title: LPA DL Le 2462

description: microRNA expression data from liver


title: LPA DL Le 2497

description: microRNA expression data from liver


title: LPA DL Le 2487

description: microRNA expression data from liver


title: LPA DL Le 2568

description: microRNA expression data from liver


title: LPA DL Le 2486

description: microRNA expression data from liver


title: LPA DL Le 2459

description: microRNA expression data from liver


title: LPA DL Le 2469

description: microRNA expression data from liver


title: LPA DL Le 2455

description: microRNA expression data from liver


title: LPA DL Le 2452

description: microRNA expression data from liver


title: LPA DL Le 2488

description: microRNA expression data from liver


title: LPA DL Le 2500

description: microRNA expression data from liver


title: LPA DL Le 2450

description: microRNA expression data from liver


title: LPA DL Le 2446

description: microRNA expression data from liver


title: LPA DL Le 2442

description: microRNA expression data from liver


title: LPA DL Le 2444

description: microRNA expression data from liver


title: LPA DL Le 2448

description: microRNA expression data from liver


title: LPA DL Le 2432

description: microRNA expression data from liver


title: LPA DL Le 2467

description: microRNA expression data from liver


title: LPA DL Le 2454

description: microRNA expression data from liver


title: LPA DL Le 2394

description: microRNA expression data from liver


title: LPA DL Le 2386

description: microRNA expression data from liver


title: LPA DL Le 2429

description: microRNA expression data from liver


title: LPA DL Le 2371

description: microRNA expression data from liver


title: LPA DL Le 2384

description: microRNA expression data from liver


title: LPA DL Le 2389

description: microRNA expression data from liver


title: LPA DL Le 2381

description: microRNA expression data from liver


title: LPA DL Le 2323

description: microRNA expression data from liver


title: LPA DL Le 2366

description: microRNA expression data from liver


title: LPA DL Le 2391

description: microRNA expression data from liver


title: LPA DL Le 2363

description: microRNA expression data from liver


title: LPA DL Le 2351

description: microRNA expression data from liver


title: LPA DL Le 2383

description: microRNA expression data from liver


title: LPA DL Le 2346

description: microRNA expression data from liver


title: LPA DL Le 2355

description: microRNA expression data from liver


title: LPA DL Le 2368

description: microRNA expression data from liver


title: LPA DL Le 2349

description: microRNA expression data from liver


title: LPA DL Le 2313

description: microRNA expression data from liver


title: LPA DL Le 2354

description: microRNA expression data from liver


title: LPA DL Le 2325

description: microRNA expression data from liver


title: LPA DL Le 2385

description: microRNA expression data from liver


title: LPA DL Le 2317

description: microRNA expression data from liver


title: LPA DL Le 2330

description: microRNA expression data from liver


title: LPA DL Le 2308

description: microRNA expression data from liver


title: LPA DL Le 2304

description: microRNA expression data from liver


title: LPA DL Le 2321

description: microRNA expression data from liver


title: LPA DL Le 2316

description: microRNA expression data from liver


title: LPA DL Le 2299

description: microRNA expression data from liver


title: LPA DL Le 2302

description: microRNA expression data from liver


title: LPA DL Le 2296

description: microRNA expression data from liver


title: LPA DL Le 2295

description: microRNA expression data from liver


title: LPA DL Le 2217

description: microRNA expression data from liver


title: LPA DL Le 2292

description: microRNA expression data from liver


title: LPA DL Le 2240

description: microRNA expression data from liver


title: LPA DL Le 2255

description: microRNA expression data from liver


title: LPA DL Le 2256

description: microRNA expression data from liver


title: LPA DL Le 2239

description: microRNA expression data from liver


title: LPA DL Le 2238

description: microRNA expression data from liver


title: LPA DL Le 2254

description: microRNA expression data from liver


title: LPA DL Le 2211

description: microRNA expression data from liver


title: LPA DL Le 2231

description: microRNA expression data from liver


title: LPA DL Le 2195

description: microRNA expression data from liver


title: LPA DL Le 2196

description: microRNA expression data from liver


title: LPA DL Le 2214

description: microRNA expression data from liver


title: LPA DL Le 2226

description: microRNA expression data from liver


title: LPA DL Le 2228

description: microRNA expression data from liver


title: LPA DL Le 2176

description: microRNA expression data from liver


title: LPA DL Le 2183

description: microRNA expression data from liver


title: LPA DL Le 2171

description: microRNA expression data from liver


title: LPA DL Le 2174

description: microRNA expression data from liver


title: LPA DL Le 2208

description: microRNA expression data from liver


title: LPA DL Le 2168

description: microRNA expression data from liver


title: LPA DL Le 2161

description: microRNA expression data from liver


title: LPA DL Le 2164

description: microRNA expression data from liver


title: LPA DL Le 2150

description: microRNA expression data from liver


title: LPA DL Le 2147

description: microRNA expression data from liver


title: LPA DL Le 2126

description: microRNA expression data from liver


title: LPA DL Le 2138

description: microRNA expression data from liver


title: LPA DL Le 2139

description: microRNA expression data from liver


title: LPA DL Le 2127

description: microRNA expression data from liver


title: LPA DL Le 2128

description: microRNA expression data from liver


title: LPA DL Le 2294

description: microRNA expression data from liver


title: LPA DL Le 2180

description: microRNA expression data from liver


title: LPA DL Le 2237

description: microRNA expression data from liver


title: LPA DL Le 2120

description: microRNA expression data from liver


title: LPA DL Le 2121

description: microRNA expression data from liver


title: LPA DL Le 2122

description: microRNA expression data from liver


title: LPA DL Le 2110

description: microRNA expression data from liver


title: LPA DL Le 2113

description: microRNA expression data from liver


title: LPA DL Le 2117

description: microRNA expression data from liver


title: LPA DL Le 2137

description: microRNA expression data from liver


title: LPA DL Le 2119

description: microRNA expression data from liver


title: LPA DL Le 2136

description: microRNA expression data from liver


title: Plasmacytoma_Filanesib-Pomalidomide-Dexamethasone_1

city: Salamanca

first: Enrique

middle: M.

last: Ocio

state: Salamanca

address: Campus Universitario Miguel de Unamuno

country: Spain

institute: Centro de investigación del cáncer

hyb_protocol: Labeled RNA was hybridized to Human Gene 2.0 ST Array

scan_protocol: Washing and scanning were performed using GeneChip System of Affymetrix (GeneChip Hybridization Oven 640, GeneChip Fluidics Station 450 and GeneChip Scanner 7G).

data_processing:
Expression value for each probe set was calculated using Affymetrix Expression Console that uses RMA (Robust Multi-Array Average) algorithm.
HuGene-2_0-st.pgf
HuGene-2_0-st.mps

source_name: Plasmacytoma, Filanesib 10mg/kg, Pomalidomide 8mg/kg, Dexamethasone 0.5mg/kg, 2 days

label_protocol: As per manufacturer's instructions (Affymetrix)

extract_protocol: Total RNA was isolated with the miRNeasy mini kit (Qiagen)

treatment_protocol: CB17- SCID mice (The Jackson Laboratory, Bar Harbor, ME, USA) were subcutaneously injected into the right flank with 3x106 MM.1S cells that had been resuspended in 100 μL of RPMI-1640 medium and 100 μL of Matrigel (BD Biosciences, San Jose, CA, USA). When tumors became palpable, mice were randomized to the control group (receiving the vehicle alone-PBS) or the different treatment groups.

value: Plasmacytoma

value: Filanesib (ARRY-520) (10 mg/kg i.p. 2 days), Pomalidomide (8 mg/kg i.p., 2 days), Dexametasone (0.5 mg/kg i.p. 2 days)


title: Plasmacytoma_Filanesib-Pomalidomide-Dexamethasone_2


title: LPA DL Le 2109

description: microRNA expression data from liver


title: Plasmacytoma_Filanesib-Pomalidomide-Dexamethasone_3


title: Plasmacytoma_Pomalidomide-Dexamethasone_3

source_name: Plasmacytoma, Pomalidomide 8mg/kg, Dexamethasone 0.5mg/kg, 2 days

value: Pomalidomide (8 mg/kg i.p., 2 days), Dexametasone (0.5 mg/kg i.p. 2 days)


title: Plasmacytoma_Pomalidomide-Dexamethasone_2


title: Plasmacytoma_Pomalidomide-Dexamethasone_1


title: LPA DL Le 2498

description: microRNA expression data from liver


title: LPA DL Le 2125

description: microRNA expression data from liver


title: Plasmacytoma_Filanesib-Pomalidomide_2

source_name: Plasmacytoma, Filanesib 10 mg/kg, Pomalidomide 8mg/kg, 2 days

value: Filanesib (ARRY-520) (10 mg/kg i.p. 2 days), Pomalidomide (8 mg/kg i.p., 2 days)


title: Plasmacytoma_Dexamethasone_3

source_name: Plasmacytoma, Dexametasone 0.5 mg/kg, 2 days

value: Dexametasone (0.5 mg/kg i.p. 2 days)


title: LPA DL Le 2434

description: microRNA expression data from liver


title: Plasmacytoma_Dexamethasone_1


title: Plasmacytoma_Filanesib-Dexamethasone_2

source_name: Plasmacytoma, Filanesib 10 mg/kg, Dexametasone 0.5 mg/kg, 2 days

value: Filanesib (ARRY-520) (10 mg/kg i.p. 2 days), Dexametasone (0.5 mg/kg i.p. 2 days)


title: Plasmacytoma_Pomalidomide_3

source_name: Plasmacytoma, Pomalidomide 8 mg/kg,  2 days

value: Pomalidomide (8 mg/kg i.p., 2 days)


title: Plasmacytoma_Pomalidomide_2


title: Plasmacytoma_Filanesib-Pomalidomide_3


title: Plasmacytoma_Filanesib-Pomalidomide_1


title: Plasmacytoma_Filanesib_1

source_name: Plasmacytoma, Filanesib 10 mg/kg, 2 days

value: Filanesib (ARRY-520) (10 mg/kg i.p. 2 days)


title: Plasmacytoma_Filanesib-Dexamethasone_3


title: Plasmacytoma_Filanesib-Dexamethasone_1


title: Plasmacytoma_Pomalidomide_1


title: Plasmacytoma_Control_3

source_name: Plasmacytoma, no treatment

value: No treatment


title: Plasmacytoma_Control_1


title: MM.1S_FPD1

source_name: MM.1S  treated with 1nM Filanesib, 500nM Pomalidomide and 10nM Dexamethasone for 24 hours

growth_protocol: Cells were cultured in RPMI-1640 medium supplemented with 2 mM L-glutamine, 100 U/mL penicillin, 100 mg/mL streptomycin and 10% fetal bovine serum at 37ºC and 5% CO2/95% air.

treatment_protocol: MM.1S cells were treated with PD, F or PDF or control vehicle for 24 h

tag: cell line

value: MM.1S cell line

value: 1 nM Filanesib and 500 nM Pomalidomide and 10 nM Dexamethasone, 24h


title: Plasmacytoma_Control_2

tag: tissue


title: Plasmacytoma_Filanesib_3


title: Plasmacytoma_Dexamethasone_2


title: MM.1S_F1

source_name: MM.1S  treated with 1nM Filanesib and 10nM Dexamethasone for 24 hours

value: 1 nM Filanesib and 10 nM Dexamethasone, 24h


title: MM.1S_PD1

source_name: MM.1S  treated with 500nM Pomalidomide and 10nM Dexamethasone for 24 hours

value: 500 nM Pomalidomide and 10 nM Dexamethasone, 24h


title: MM.1S_FPD3


title: MM.1S_F3


title: MM.1S_PD2


title: MM.1S_Control_3

source_name: MM.1S  untreated

value: Vehicle


title: MM.1S_PD3


title: MM.1S_F2


title: MM.1S_FPD2


title: MM.1S_Control_2


title: MM.1S_Control_1


title: LC-W

city: Kamakura

first: Satoshi

last: Kondo

state: Kanagawa

address: Tebiro 6-10-1

department: New Projects Development Division

country: Japan

institute: Toray Industries,Inc.

description: Total RNA containing small RNA was pooled from 10 EGFR-wild-type patients

hyb_protocol: Hybridized for 16 h at 32 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..

scan_protocol: 3D-Gene Scanner ((Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction(Toray Industries Inc., Tokyo, Japan).

data_processing: The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95% confidence intervals.

source_name: LA-male-smoker-EGFR-wt pooled

label_protocol: Extracted total RNA was labeled with Cy5 using the 3D-Gene miRNA labeling kit (Toray Industries Inc., Tokyo, Japan).

extract_protocol: Total RNA containing small RNA was extracted using TRIsol® reagent (Thermo Fisher Scientific, USA) according to the manufacturer’s protocol.

value: primary lung cancer

tag: histology

value: Lung Adenocarcinoma

tag: Sex

value: male

tag: smoking

value: Smoker

tag: egfr mutation status

value: Wild-Type

tag: Stage

value: IA and IB

tag: therapy

value: no treatment


title: Data Heifer Rep4

city: Quebec

middle: André

last: Landry

state: Quebec

address: 2440 boul. Hochelaga

department: Animal Science

country: Canada

institute: University Laval

description: Rep 4 of 4

hyb_protocol: The hybridization of microarray slides 4x44K printed with oligonucleotides by Agilent for all EmbryoGENE related projects using the expression array protocol.

scan_protocol: Scan on a PowerScanner (Tecan) using the autogain function. Images were quantified using ArrayPro version 6.3 (MediaCybernetics)

data_processing: Background subtracted, Loess and Quantile normalized data obtained from log2 of processed Red signal/processed Green signal. Flexarray software used.

organism: Bos taurus

source_name: Pool#12_Poor Donors_GranulosaCells

label_protocol:
[Amp protocol] To generate enough material for hybridization, the samples were linearly amplified. Antisense RNA (aRNA) was produced using the RiboAmp®HSPlus RNA Amplification kit (Thermo fisher Scientific). After two amplification rounds of 6 hr each, the aRNA output was quantified using the NanoDrop ND-1000 (Nano-Drop Technologies, Wilmington, DE).
For each sample, aRNA was labeled using the ULS Fluorescent Labeling Kit for Agilent arrays (Cy3/Cy5) (Kreatech Diagnostics, Amsterdam, Netherlands). The labeled product was then purified with the Pico-Pure RNA Isolation Kit.

growth_protocol: [Embryo production] In vitro maturation (IVM), in vitro fertilization (IVF), in vitro culture (IVC)

extract_protocol: Total RNA was extracted from the pools using the AllPrep DNA/RNA micro kit, following the Parallel gDNA and total RNA extraction protocol. Total RNA integrity and concentration were evaluated on an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA) with the RNA PicoLab Chip (Agilent Technologies).

tag: sample type

tag: breed (cattle)

value: Holstein

tag: oocyte developmental competence

value: Poor

value: Granulosa Cells

source_name: Pool#4_Good Donors_GranulosaCells

value: Good


title: LC-M

description: Total RNA containing small RNA was pooled from 10 EGFR-mutant patients

source_name: LA-male-smoker-EGFR-mut pooled

value: Mutant


title: BP-M

description: The blood plasmas were pooled from 10 EGFR-mutant patients

extract_protocol: RNA samples were extracted from serum using 3D-Gene extraction reagent from liquid sample(Toray Industries Inc., Tokyo, Japan).

value: plasma


title: BP-W

description: The blood plasmas were pooled from 10 EGFR-wild-type patients


title: Plasmacytoma_Filanesib_2

value: Plasmacytoma

value: Filanesib (ARRY-520) (10 mg/kg i.p. 2 days)


title: Data Heifer Rep1

description: Rep 1 of 4

source_name: Pool#1_Good Donors_GranulosaCells

tag: cell type

source_name: Pool#9_Poor Donors_GranulosaCells


title: hDicer-TRBP-pre-let-7-DMSO

city: Qingdao

first: Lei

last: Sun

state: Shandong

address: Shandong street

institute: Shandong University

data_processing:
Library strategy: icSHAPE
remove duplicete reads as icshape pipeline
trimmed reads adaptor as icshape pipeline
mapping reads to pre-let-7 with botie2 as default parameters
calculate rpkm as icshape pipeline
calulate reverse transcript stop sites as icshape pipeline
calculate icSHAPE score as icshape pipeline
Genome_build: pre-let-7
Supplementary_files_format_and_content: tab-delimited text icshape score  for icshape file

extract_protocol:
35 µl of buffer RLT was added to stop the reaction on ice. The RNA was purified with an RNeasy mini column and eluted with 50 µl of pure water twice (100-µl final volume).
The modified RNA was captured with DIBO-biotin at 37 °C for 2h incubation and then purified with the Zymo RNA kit. It was then subjected to end repair, linker ligation and reverse transcription. Transcribed cDNA was enriched by biotin together with the RNA moiety. cDNA libraries were amplified using qPCR with about 13-15 cycles.

treatment_protocol: The pre-let-7 RNA (5ʹ-UGAGGUAGUAGGUUGUAUAGUUUUAGGGUCACACCCACCACUGGGAGAUAACUAUACAAUCUACUGUCUUACC-3ʹ) was synthesized by Genescript Inc. In order to form the hairpin pre-let-7, we heated the RNA at 90 °C for 3 min then snap-cooled it on ice for 5 min. In order to reconstitute the hDicer-TRBP-pre-let-7 complex, we mixed ~200 µg (0.8 nmol) hDicer-TRBP complex and ~54 µg (2.3 nmol) pre-let-7 RNA in 50 µl of gel filtration buffer containing 2 mM Ca2+ and incubated the mixture on ice for 40 min. After this, the mixture was applied to a Superose™ 6 Increase 5/150 GL (GE Healthcare) column installed on an ÄKTAmicro (GE Healthcare) equilibrated in gel filtration buffer containing 2 mM Ca2+. Fractions containing the hDicer-TRBP-pre-let-7 complex were collected at a final concentration of ~0.4 mg/ml. NAI-N3 modified the free pre-let-7 and hDicer-TRBP-pre-let-7 complex for 10 min in 37°C

tag: group

value: hDicer-TRBP-pre-let-7 complex


title: hDicer0TRBP-pre-let-7-NAI


title: pre-let-7-NAI


title: Data Heifer Rep2

description: Rep 2 of 4

source_name: Pool#2_Good Donors_GranulosaCells

source_name: Pool#10_Poor Donors_GranulosaCells


title: Dex.MntBP.rep2

city: Camperdown, Sydney

first: Rima

last: Chaudhuri

state: NSW

address: D17 Johns Hopkins Drive

department: Charles Perkins Center

country: Australia

institute: University of Sydney

hyb_protocol: Fifteen micrograms of labeled cRNA was fragmented by incubating at 94°C for 30 min in 40 mM Tris-acetate pH 8.1, 100 mM potassium acetate and 30 mM magnesium acetate. Ten micrograms of fragmented cRNA was then hybridized to Mouse Genome 430 2.0 arrays at 45°C for 19 hours in a rotisserie oven set at 60 rpm.

scan_protocol: Arrays were washed and stained in the Affymetrix Fluidics station and scanned on GeneChip scanner 3000.

data_processing: Data analysis files were generated using manufacturer’s GeneChip Command Console system (Affymterix).

source_name: insulin resistant 3T3-L1 adipose cells

label_protocol: The methods for preparation of labeled cRNA and array hybridization were provided by Affymetrix (Santa Clara, CA). Briefly, 3 ug of total RNA was converted into double-stranded cDNA using a cDNA synthesis kit SuperScript Choice (Invitrogen, Carlsbad, CA) and a T7-(dT)24 oligomer primer (Integrated DNA Technologies, Coralville, IO). Double-stranded cDNA was purified using affinity resin Sample Cleanup Module Kit (Affymetrix). Labeled cRNA was generated using a T7 RNA polymerase and biotin-labeled nucleotides in an in vitro transcription reaction (Enzo Diagnostics, Farmingdale, NY). The labeled cRNA was purified using Sample Cleanup Module Kit (Affymetrix).

growth_protocol: 3T3-L1 preadipocytes were differentiated into adipocytes using established protocols (PMID: 10926832).

extract_protocol: The cells were harvested and total RNA was isolated using Qiagen Kit.

treatment_protocol: 3T3-L1 adipocytes were made insulin resistant with CI, TNF, and Dex models as reported in PMID 19805130. MnTBAP was added to cells during the final 6 hours of treatment (PMID 19805130).

value: 3T3-L1 preadipocytes


title: pre-let-7-DMSO


title: CI.MnTBP.rep1


title: TNF.MnTBP.rep1


title: Data Heifer Rep3

description: Rep 3 of 4

source_name: Pool#11_Poor Donors_GranulosaCells

source_name: Pool#3_Good Donors_GranulosaCells


title: SpColCtrl_rep2a2239

city: Boston

first: CBDM

last: Lab

state: MA

address: 77 Avenue Louis Pasteur

department: Microbiology and Immunobiology

institute: Harvard Medical School

data_processing:
fastq quality read filtering (>80% with Sanger Q>20)
cell barcode demultiplexing (custom script)
mapping with tophat2 on the mm10 transcriptome
filtering out low alignment score reads and multiple mapping reads (samtools)
duplication filtering using unique molecular identifiers (custom scripts). UMI sequence is at the 4rth position in the read ID (separation by ":")
Genome_build: mm10
Supplementary_files_format_and_content: raw read counts

source_name: spleen

growth_protocol: Live cells were sorted by flow cytometry from the spleen and colon lamina propria of mice (carrying a Foxp3 reporter gene).

extract_protocol:
Spleen were mechanically dissociated and colon were digested with collagenase/dispase to single cell suspensions. Tregs were sorted by flow cytometry as DAPI- TCRb+ CD4+ GFP+ cells. Single cells were then encapsulated in nanodroplets according to the InDrops protocol.
mRNA was reverse transcribed using adapters containing oligo dT in each droplet. The emulsion was then broken and linear amplification was performed by in vitro transcription. Illumina adapters were added by reverse transcription and PCR. Library were sequenced using a NextSeq500.

value: spleen

tag: library name

tag: strain

tag: age (months)

tag: total number of reads

tag: number of mapped reads

tag: number of unique reads (umi)


title: TNF.MnTBP.rep3

tag: tissue


title: SpColCtrl_rep2a2238

tag: Sex


title: 3t3-L1.GO.rep2

treatment_protocol: Glucose oxidase treatment protocol is described in PMID 18460333.


title: Dex.MntBP.rep3


title: 3t3-L1.GO.rep1


title: CI.MnTBP.rep2


title: 3t3-L1.GO.rep3


title: TNF.MnTBP.rep2


title: SpColCtrl_rep2a2235


title: LPA DL Le 2111

description: microRNA expression data from liver

source_name: Liver

tag: age

value: adult

tag: body weight

tag: meat quality grade


title: SpColCtrl_rep2a2231

value: male


title: SpColCtrl_rep2a2234


title: SpColCtrl_rep2a2229


title: SpColCtrl_rep2a2236


title: SpColCtrl_rep2a2232


title: SpColCtrl_rep2a2233


title: SpColCtrl_rep2a2230


title: SpColCtrl_rep2a2228


title: SpColCtrl_rep2a2225


title: SpColCtrl_rep2a2237


title: SpColCtrl_rep2a2227


title: SpColCtrl_rep2a2221


title: SpColCtrl_rep2a2223


title: Dex.MnTBP.rep1


title: SpColCtrl_rep2a2226


title: SpColCtrl_rep2a2220


title: SpColCtrl_rep2a2222


title: SpColCtrl_rep2a2219


title: SpColCtrl_rep2a2218


title: SpColCtrl_rep2a2213


title: SpColCtrl_rep2a2216


title: SpColCtrl_rep2a2214


title: SpColCtrl_rep2a2217


title: SpColCtrl_rep2a2212


title: SpColCtrl_rep2a2215


title: SpColCtrl_rep2a2211


title: SpColCtrl_rep2a2210


title: SpColCtrl_rep2a2209


title: SpColCtrl_rep2a2224


title: SpColCtrl_rep2a2207


title: SpColCtrl_rep2a2204


title: SpColCtrl_rep2a2206


title: SpColCtrl_rep2a2199


title: SpColCtrl_rep2a2202


title: SpColCtrl_rep2a2205


title: SpColCtrl_rep2a2201


title: SpColCtrl_rep2a2203


title: SpColCtrl_rep2a2198


title: SpColCtrl_rep2a2200


title: SpColCtrl_rep2a2192


title: SpColCtrl_rep2a2191


title: SpColCtrl_rep2a2196


title: SpColCtrl_rep2a2195


title: SpColCtrl_rep2a2197


title: SpColCtrl_rep2a2193


title: SpColCtrl_rep2a2189


title: SpColCtrl_rep2a2194


title: SpColCtrl_rep2a2185


title: SpColCtrl_rep2a2184


title: SpColCtrl_rep2a2187


title: SpColCtrl_rep2a2186


title: SpColCtrl_rep2a2190


title: SpColCtrl_rep2a2208


title: SpColCtrl_rep2a2183


title: SpColCtrl_rep2a2182


title: SpColCtrl_rep2a2188


title: SpColCtrl_rep2a2181


title: SpColCtrl_rep2a2180


title: SpColCtrl_rep2a2178


title: SpColCtrl_rep2a2174


title: SpColCtrl_rep2a2176


title: SpColCtrl_rep2a2179


title: SpColCtrl_rep2a2175


title: SpColCtrl_rep2a2168


title: SpColCtrl_rep2a2172


title: SpColCtrl_rep2a2166


title: SpColCtrl_rep2a2164


title: SpColCtrl_rep2a2171


title: SpColCtrl_rep2a2167


title: SpColCtrl_rep2a2165


title: SpColCtrl_rep2a2170


title: SpColCtrl_rep2a2173


title: SpColCtrl_rep2a2169


title: SpColCtrl_rep2a2162


title: SpColCtrl_rep2a2163


title: SpColCtrl_rep2a2158


title: SpColCtrl_rep2a2160


title: SpColCtrl_rep2a2159


title: SpColCtrl_rep2a2157


title: SpColCtrl_rep2a2156


title: SpColCtrl_rep2a2154


title: SpColCtrl_rep2a2153


title: SpColCtrl_rep2a2177


title: SpColCtrl_rep2a2152


title: SpColCtrl_rep2a2151


title: SpColCtrl_rep2a2150


title: SpColCtrl_rep2a2149


title: SpColCtrl_rep2a2146


title: SpColCtrl_rep2a2145


title: SpColCtrl_rep2a2143


title: SpColCtrl_rep2a2141


title: SpColCtrl_rep2a2142


title: SpColCtrl_rep2a2161


title: SpColCtrl_rep2a2148


title: SpColCtrl_rep2a2134


title: SpColCtrl_rep2a2144


title: SpColCtrl_rep2a2135


title: SpColCtrl_rep2a2140


title: SpColCtrl_rep2a2138


title: SpColCtrl_rep2a2136


title: SpColCtrl_rep2a2155


title: SpColCtrl_rep2a2133


title: SpColCtrl_rep2a2131


title: SpColCtrl_rep2a2130


title: SpColCtrl_rep2a2132


title: SpColCtrl_rep2a2137


title: SpColCtrl_rep2a2128


title: SpColCtrl_rep2a2129


title: SpColCtrl_rep2a2127


title: SpColCtrl_rep2a2126


title: SpColCtrl_rep2a2125


title: SpColCtrl_rep2a2147


title: SpColCtrl_rep2a2124


title: SpColCtrl_rep2a2139


title: SpColCtrl_rep2a2122


title: SpColCtrl_rep2a2120


title: SpColCtrl_rep2a2121


title: SpColCtrl_rep2a2118


title: SpColCtrl_rep2a2117


title: SpColCtrl_rep2a2110


title: SpColCtrl_rep2a2115


title: SpColCtrl_rep2a2111


title: SpColCtrl_rep2a2113


title: SpColCtrl_rep2a2108


title: SpColCtrl_rep2a2114


title: SpColCtrl_rep2a2105


title: SpColCtrl_rep2a2107


title: SpColCtrl_rep2a2116


title: SpColCtrl_rep2a2103


title: SpColCtrl_rep2a2101


title: SpColCtrl_rep2a2104


title: SpColCtrl_rep2a2109


title: SpColCtrl_rep2a2102


title: SpColCtrl_rep2a2106


title: SpColCtrl_rep2a2100


title: SpColCtrl_rep2a2099


title: SpColCtrl_rep2a2098


title: SpColCtrl_rep2a2097


title: SpColCtrl_rep2a2096


title: SpColCtrl_rep2a2119


title: SpColCtrl_rep2a2095


title: SpColCtrl_rep2a2093


title: SpColCtrl_rep2a2094


title: SpColCtrl_rep2a2092


title: SpColCtrl_rep2a2091


title: SpColCtrl_rep2a2112


title: SpColCtrl_rep2a2089


title: SpColCtrl_rep2a2083


title: SpColCtrl_rep2a2090


title: SpColCtrl_rep2a2080


title: SpColCtrl_rep2a2077


title: SpColCtrl_rep2a2082


title: SpColCtrl_rep2a2076


title: SpColCtrl_rep2a2088


title: SpColCtrl_rep2a2087


title: SpColCtrl_rep2a2078


title: SpColCtrl_rep2a2086


title: SpColCtrl_rep2a2081


title: SpColCtrl_rep2a2075


title: SpColCtrl_rep2a2085


title: SpColCtrl_rep2a2084


title: SpColCtrl_rep2a2074


title: SpColCtrl_rep2a2072


title: SpColCtrl_rep2a2069


title: SpColCtrl_rep2a2073


title: SpColCtrl_rep2a2079


title: SpColCtrl_rep2a2068


title: SpColCtrl_rep2a2070


title: SpColCtrl_rep2a2071


title: SpColCtrl_rep2a2067


title: SpColCtrl_rep2a2066


title: SpColCtrl_rep2a2065


title: SpColCtrl_rep2a2064


title: SpColCtrl_rep2a2063


title: SpColCtrl_rep2a2060


title: SpColCtrl_rep2a2062


title: SpColCtrl_rep2a2057


title: SpColCtrl_rep2a2061


title: SpColCtrl_rep2a2058


title: SpColCtrl_rep2a2053


title: SpColCtrl_rep2a2050


title: SpColCtrl_rep2a2051


title: SpColCtrl_rep2a2056


title: SpColCtrl_rep2a2052


title: SpColCtrl_rep2a2054


title: SpColCtrl_rep2a2059


title: SpColCtrl_rep2a2047


title: SpColCtrl_rep2a2048


title: SpColCtrl_rep2a2046


title: SpColCtrl_rep2a2049


title: SpColCtrl_rep2a2045


title: SpColCtrl_rep2a2044


title: SpColCtrl_rep2a2043


title: SpColCtrl_rep2a2042


title: SpColCtrl_rep2a2041


title: SpColCtrl_rep2a2040


title: SpColCtrl_rep2a2039


title: SpColCtrl_rep2a2038


title: SpColCtrl_rep2a2036


title: SpColCtrl_rep2a2035


title: SpColCtrl_rep2a2037


title: SpColCtrl_rep2a2034


title: SpColCtrl_rep2a2033


title: SpColCtrl_rep2a2030


title: SpColCtrl_rep2a2029


title: SpColCtrl_rep2a2055


title: SpColCtrl_rep2a2023


title: SpColCtrl_rep2a2024


title: SpColCtrl_rep2a2025


title: SpColCtrl_rep2a2021


title: SpColCtrl_rep2a2019


title: SpColCtrl_rep2a2017


title: SpColCtrl_rep2a2028


title: SpColCtrl_rep2a2031


title: SpColCtrl_rep2a2018


title: SpColCtrl_rep2a2016


title: SpColCtrl_rep2a2015


title: SpColCtrl_rep2a2014


title: SpColCtrl_rep2a2013


title: SpColCtrl_rep2a2010


title: SpColCtrl_rep2a2032


title: SpColCtrl_rep2a2026


title: SpColCtrl_rep2a2027


title: SpColCtrl_rep2a2007


title: SpColCtrl_rep2a2006


title: SpColCtrl_rep2a2022


title: SpColCtrl_rep2a2020


title: SpColCtrl_rep2a2003


title: SpColCtrl_rep2a2004


title: LPA DL Le 2458

description: microRNA expression data from liver


title: SpColCtrl_rep2a2002


title: SpColCtrl_rep2a2000


title: SpColCtrl_rep2a2001


title: SpColCtrl_rep2a1997


title: SpColCtrl_rep2a1996


title: SpColCtrl_rep2a2011


title: SpColCtrl_rep2a1992


title: SpColCtrl_rep2a2008


title: SpColCtrl_rep2a2012


title: SpColCtrl_rep2a1993


title: SpColCtrl_rep2a1998


title: SpColCtrl_rep2a1994


title: SpColCtrl_rep2a1995


title: SpColCtrl_rep2a1990


title: SpColCtrl_rep2a1991


title: SpColCtrl_rep2a2009


title: SpColCtrl_rep2a1988


title: SpColCtrl_rep2a2005


title: SpColCtrl_rep2a1987


title: SpColCtrl_rep2a1985


title: SpColCtrl_rep2a1986


title: SpColCtrl_rep2a1983


title: SpColCtrl_rep2a1999


title: SpColCtrl_rep2a1984


title: SpColCtrl_rep2a1982


title: SpColCtrl_rep2a1979


title: SpColCtrl_rep2a1981


title: SpColCtrl_rep2a1989


title: SpColCtrl_rep2a1972


title: SpColCtrl_rep2a1968


title: SpColCtrl_rep2a1971


title: SpColCtrl_rep2a1975


title: SpColCtrl_rep2a1973


title: SpColCtrl_rep2a1967


title: SpColCtrl_rep2a1966


title: SpColCtrl_rep2a1969


title: SpColCtrl_rep2a1970


title: SpColCtrl_rep2a1962


title: SpColCtrl_rep2a1963


title: SpColCtrl_rep2a1965


title: SpColCtrl_rep2a1960


title: SpColCtrl_rep2a1961


title: SpColCtrl_rep2a1958


title: SpColCtrl_rep2a1980


title: SpColCtrl_rep2a1964


title: SpColCtrl_rep2a1957


title: SpColCtrl_rep2a1976


title: SpColCtrl_rep2a1977


title: SpColCtrl_rep2a1952


title: SpColCtrl_rep2a1978


title: SpColCtrl_rep2a1954


title: SpColCtrl_rep2a1953


title: SpColCtrl_rep2a1974


title: SpColCtrl_rep2a1956


title: SpColCtrl_rep2a1951


title: SpColCtrl_rep2a1950


title: SpColCtrl_rep2a1948


title: SpColCtrl_rep2a1945


title: SpColCtrl_rep2a1947


title: SpColCtrl_rep2a1946


title: SpColCtrl_rep2a1943


title: SpColCtrl_rep2a1942


title: SpColCtrl_rep2a1941


title: SpColCtrl_rep2a1959


title: SpColCtrl_rep2a1938


title: SpColCtrl_rep2a1939


title: SpColCtrl_rep2a1955


title: SpColCtrl_rep2a1932


title: SpColCtrl_rep2a1940


title: SpColCtrl_rep2a1934


title: SpColCtrl_rep2a1935


title: SpColCtrl_rep2a1937


title: SpColCtrl_rep2a1931


title: SpColCtrl_rep2a1936


title: SpColCtrl_rep2a1933


title: SpColCtrl_rep2a1929


title: SpColCtrl_rep2a1930


title: SpColCtrl_rep2a1927


title: SpColCtrl_rep2a1926


title: SpColCtrl_rep2a1928


title: SpColCtrl_rep2a1925


title: SpColCtrl_rep2a1924


title: SpColCtrl_rep2a1944


title: SpColCtrl_rep2a1923


title: SpColCtrl_rep2a1922


title: SpColCtrl_rep2a1921


title: SpColCtrl_rep2a1949


title: SpColCtrl_rep2a1918


title: SpColCtrl_rep2a1919


title: SpColCtrl_rep2a1917


title: SpColCtrl_rep2a1915


title: SpColCtrl_rep2a1916


title: SpColCtrl_rep2a1920


title: SpColCtrl_rep2a1913


title: SpColCtrl_rep2a1914


title: SpColCtrl_rep2a1911


title: SpColCtrl_rep2a1907


title: SpColCtrl_rep2a1912


title: SpColCtrl_rep2a1910


title: SpColCtrl_rep2a1906


title: SpColCtrl_rep2a1908


title: SpColCtrl_rep2a1903


title: SpColCtrl_rep2a1902


title: SpColCtrl_rep2a1898


title: SpColCtrl_rep2a1904


title: SpColCtrl_rep2a1905


title: SpColCtrl_rep2a1901


title: SpColCtrl_rep2a1899


title: SpColCtrl_rep2a1900


title: SpColCtrl_rep2a1896


title: SpColCtrl_rep2a1897


title: SpColCtrl_rep2a1895


title: SpColCtrl_rep2a1894


title: SpColCtrl_rep2a1890


title: SpColCtrl_rep2a1889


title: SpColCtrl_rep2a1892


title: SpColCtrl_rep2a1893


title: SpColCtrl_rep2a1886


title: SpColCtrl_rep2a1885


title: SpColCtrl_rep2a1888


title: SpColCtrl_rep2a1909


title: SpColCtrl_rep2a1887


title: SpColCtrl_rep2a1883


title: SpColCtrl_rep2a1881


title: SpColCtrl_rep2a1884


title: SpColCtrl_rep2a1880


title: SpColCtrl_rep2a1882


title: SpColCtrl_rep2a1879


title: SpColCtrl_rep2a1876


title: SpColCtrl_rep2a1877


title: SpColCtrl_rep2a1874


title: SpColCtrl_rep2a1878


title: SpColCtrl_rep2a1875


title: SpColCtrl_rep2a1872


title: SpColCtrl_rep2a1873


title: SpColCtrl_rep2a1869


title: SpColCtrl_rep2a1870


title: SpColCtrl_rep2a1871


title: SpColCtrl_rep2a1868


title: SpColCtrl_rep2a1867


title: SpColCtrl_rep2a1866


title: SpColCtrl_rep2a1891


title: SpColCtrl_rep2a1863


title: SpColCtrl_rep2a1865


title: SpColCtrl_rep2a1859


title: SpColCtrl_rep2a1862


title: SpColCtrl_rep2a1860


title: SpColCtrl_rep2a1858


title: SpColCtrl_rep2a1855


title: SpColCtrl_rep2a1854


title: SpColCtrl_rep2a1856


title: SpColCtrl_rep2a1857


title: SpColCtrl_rep2a1864


title: SpColCtrl_rep2a1853


title: SpColCtrl_rep2a1852


title: SpColCtrl_rep2a1850


title: SpColCtrl_rep2a1851


title: SpColCtrl_rep2a1844


title: SpColCtrl_rep2a1849


title: SpColCtrl_rep2a1845


title: SpColCtrl_rep2a1846


title: SpColCtrl_rep2a1847


title: SpColCtrl_rep2a1848


title: SpColCtrl_rep2a1843


title: SpColCtrl_rep2a1861


title: SpColCtrl_rep2a1842


title: SpColCtrl_rep2a1840


title: SpColCtrl_rep2a1838


title: SpColCtrl_rep2a1841


title: SpColCtrl_rep2a1836


title: SpColCtrl_rep2a1837


title: SpColCtrl_rep2a1835


title: SpColCtrl_rep2a1831


title: SpColCtrl_rep2a1834


title: SpColCtrl_rep2a1830


title: SpColCtrl_rep2a1829


title: SpColCtrl_rep2a1833


title: SpColCtrl_rep2a1827


title: SpColCtrl_rep2a1828


title: SpColCtrl_rep2a1826


title: SpColCtrl_rep2a1822


title: SpColCtrl_rep2a1825


title: SpColCtrl_rep2a1823


title: SpColCtrl_rep2a1824


title: SpColCtrl_rep2a1816


title: SpColCtrl_rep2a1819


title: SpColCtrl_rep2a1821


title: SpColCtrl_rep2a1818


title: SpColCtrl_rep2a1817


title: SpColCtrl_rep2a1839


title: SpColCtrl_rep2a1813


title: SpColCtrl_rep2a1820


title: SpColCtrl_rep2a1814


title: SpColCtrl_rep2a1832


title: SpColCtrl_rep2a1815


title: SpColCtrl_rep2a1811


title: SpColCtrl_rep2a1812


title: SpColCtrl_rep2a1810


title: SpColCtrl_rep2a1807


title: SpColCtrl_rep2a1808


title: SpColCtrl_rep2a1805


title: SpColCtrl_rep2a1804


title: SpColCtrl_rep2a1806


title: SpColCtrl_rep2a1803


title: SpColCtrl_rep2a1801


title: SpColCtrl_rep2a1800


title: SpColCtrl_rep2a1799


title: SpColCtrl_rep2a1797


title: SpColCtrl_rep2a1798


title: SpColCtrl_rep2a1795


title: SpColCtrl_rep2a1796


title: SpColCtrl_rep2a1792


title: SpColCtrl_rep2a1794


title: SpColCtrl_rep2a1793


title: SpColCtrl_rep2a1789


title: SpColCtrl_rep2a1791


title: SpColCtrl_rep2a1809


title: SpColCtrl_rep2a1790


title: SpColCtrl_rep2a1788


title: SpColCtrl_rep2a1787


title: SpColCtrl_rep2a1786


title: SpColCtrl_rep2a1780


title: SpColCtrl_rep2a1782


title: SpColCtrl_rep2a1779


title: SpColCtrl_rep2a1802


title: SpColCtrl_rep2a1783


title: SpColCtrl_rep2a1784


title: SpColCtrl_rep2a1781


title: SpColCtrl_rep2a1778


title: SpColCtrl_rep2a1785


title: SpColCtrl_rep2a1776


title: SpColCtrl_rep2a1777


title: SpColCtrl_rep2a1775


title: SpColCtrl_rep2a1773


title: SpColCtrl_rep2a1774


title: SpColCtrl_rep2a1770


title: SpColCtrl_rep2a1768


title: SpColCtrl_rep2a1769


title: SpColCtrl_rep2a1772


title: SpColCtrl_rep2a1767


title: SpColCtrl_rep2a1766


title: SpColCtrl_rep2a1765


title: SpColCtrl_rep2a1762


title: SpColCtrl_rep2a1763


title: SpColCtrl_rep2a1761


title: SpColCtrl_rep2a1764


title: SpColCtrl_rep2a1759


title: SpColCtrl_rep2a1760


title: SpColCtrl_rep2a1756


title: SpColCtrl_rep2a1755


title: SpColCtrl_rep2a1751


title: SpColCtrl_rep2a1752


title: SpColCtrl_rep2a1771


title: SpColCtrl_rep2a1757


title: SpColCtrl_rep2a1758


title: SpColCtrl_rep2a1749


title: SpColCtrl_rep2a1754


title: SpColCtrl_rep2a1748


title: SpColCtrl_rep2a1750


title: SpColCtrl_rep2a1753


title: SpColCtrl_rep2a1745


title: SpColCtrl_rep2a1747


title: SpColCtrl_rep2a1740


title: SpColCtrl_rep2a1743


title: SpColCtrl_rep2a1741


title: SpColCtrl_rep2a1744


title: SpColCtrl_rep2a1739


title: SpColCtrl_rep2a1746


title: SpColCtrl_rep2a1738


title: SpColCtrl_rep2a1737


title: SpColCtrl_rep2a1734


title: SpColCtrl_rep2a1735


title: SpColCtrl_rep2a1732


title: SpColCtrl_rep2a1736


title: SpColCtrl_rep2a1731


title: SpColCtrl_rep2a1726


title: SpColCtrl_rep2a1733


title: SpColCtrl_rep2a1730


title: SpColCtrl_rep2a1727


title: SpColCtrl_rep2a1729


title: SpColCtrl_rep2a1728


title: SpColCtrl_rep2a1725


title: SpColCtrl_rep2a1723


title: SpColCtrl_rep2a1724


title: SpColCtrl_rep2a1742


title: SpColCtrl_rep2a1722


title: SpColCtrl_rep2a1721


title: SpColCtrl_rep2a1714


title: SpColCtrl_rep2a1716


title: SpColCtrl_rep2a1718


title: SpColCtrl_rep2a1720


title: SpColCtrl_rep2a1719


title: SpColCtrl_rep2a1715


title: SpColCtrl_rep2a1717


title: SpColCtrl_rep2a1708


title: SpColCtrl_rep2a1707


title: SpColCtrl_rep2a1705


title: SpColCtrl_rep2a1704


title: SpColCtrl_rep2a1706


title: SpColCtrl_rep2a1702


title: SpColCtrl_rep2a1703


title: SpColCtrl_rep2a1699


title: SpColCtrl_rep2a1701


title: SpColCtrl_rep2a1695


title: SpColCtrl_rep2a1696


title: SpColCtrl_rep2a1713


title: SpColCtrl_rep2a1700


title: SpColCtrl_rep2a1694


title: SpColCtrl_rep2a1692


title: SpColCtrl_rep2a1712


title: SpColCtrl_rep2a1693


title: SpColCtrl_rep2a1686


title: SpColCtrl_rep2a1685


title: SpColCtrl_rep2a1689


title: SpColCtrl_rep2a1688


title: SpColCtrl_rep2a1711


title: SpColCtrl_rep2a1710


title: SpColCtrl_rep2a1709


title: SpColCtrl_rep2a1684


title: SpColCtrl_rep2a1697


title: SpColCtrl_rep2a1681


title: SpColCtrl_rep2a1682


title: SpColCtrl_rep2a1679


title: SpColCtrl_rep2a1678


title: SpColCtrl_rep2a1680


title: SpColCtrl_rep2a1698


title: SpColCtrl_rep2a1690


title: SpColCtrl_rep2a1691


title: SpColCtrl_rep2a1677


title: SpColCtrl_rep2a1673


title: SpColCtrl_rep2a1675


title: SpColCtrl_rep2a1672


title: SpColCtrl_rep2a1687


title: SpColCtrl_rep2a1674


title: SpColCtrl_rep2a1671


title: SpColCtrl_rep2a1670


title: SpColCtrl_rep2a1668


title: SpColCtrl_rep2a1669


title: SpColCtrl_rep2a1664


title: SpColCtrl_rep2a1666


title: SpColCtrl_rep2a1662


title: SpColCtrl_rep2a1683


title: SpColCtrl_rep2a1661


title: SpColCtrl_rep2a1663


title: SpColCtrl_rep2a1676


title: SpColCtrl_rep2a1659


title: SpColCtrl_rep2a1656


title: SpColCtrl_rep2a1657


title: SpColCtrl_rep2a1655


title: SpColCtrl_rep2a1652


title: SpColCtrl_rep2a1645


title: SpColCtrl_rep2a1648


title: SpColCtrl_rep2a1647


title: SpColCtrl_rep2a1650


title: SpColCtrl_rep2a1651


title: SpColCtrl_rep2a1653


title: SpColCtrl_rep2a1644


title: SpColCtrl_rep2a1649


title: SpColCtrl_rep2a1646


title: SpColCtrl_rep2a1667


title: SpColCtrl_rep2a1665


title: SpColCtrl_rep2a1643


title: SpColCtrl_rep2a1642


title: SpColCtrl_rep2a1641


title: SpColCtrl_rep2a1660


title: SpColCtrl_rep2a1636


title: SpColCtrl_rep2a1637


title: SpColCtrl_rep2a1635


title: SpColCtrl_rep2a1638


title: SpColCtrl_rep2a1658


title: SpColCtrl_rep2a1654


title: SpColCtrl_rep2a1633


title: SpColCtrl_rep2a1632


title: SpColCtrl_rep2a1634


title: SpColCtrl_rep2a1631


title: SpColCtrl_rep2a1630


title: SpColCtrl_rep2a1625


title: SpColCtrl_rep2a1629


title: SpColCtrl_rep2a1627


title: SpColCtrl_rep2a1623


title: SpColCtrl_rep2a1624


title: SpColCtrl_rep2a1628


title: SpColCtrl_rep2a1621


title: SpColCtrl_rep2a1640


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title: SpColCtrl_rep2a1622


title: SpColCtrl_rep2a1620


title: SpColCtrl_rep2a1617


title: SpColCtrl_rep2a1618


title: SpColCtrl_rep2a1619


title: SpColCtrl_rep2a1615


title: SpColCtrl_rep2a1616


title: SpColCtrl_rep2a1612


title: SpColCtrl_rep2a1609


title: SpColCtrl_rep2a1610


title: SpColCtrl_rep2a1614


title: SpColCtrl_rep2a1608


title: SpColCtrl_rep2a1613


title: SpColCtrl_rep2a1611


title: SpColCtrl_rep2a1607


title: SpColCtrl_rep2a1626


title: SpColCtrl_rep2a1605


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title: SpColCtrl_rep2a1600


title: SpColCtrl_rep2a1604


title: SpColCtrl_rep2a1603


title: SpColCtrl_rep2a1599


title: SpColCtrl_rep2a1598


title: SpColCtrl_rep2a1597


title: SpColCtrl_rep2a1602


title: SpColCtrl_rep2a1596


title: SpColCtrl_rep2a1601


title: SpColCtrl_rep2a1594


title: SpColCtrl_rep2a1592


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title: SpColCtrl_rep2a1588


title: SpColCtrl_rep2a1589


title: SpColCtrl_rep2a1586


title: SpColCtrl_rep2a1590


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title: SpColCtrl_rep2a1584


title: SpColCtrl_rep2a1582


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title: SpColCtrl_rep2a1583


title: SpColCtrl_rep2a1581


title: SpColCtrl_rep2a1580


title: SpColCtrl_rep2a1578


title: SpColCtrl_rep2a1577


title: SpColCtrl_rep2a1576


title: SpColCtrl_rep2a1575


title: SpColCtrl_rep2a1571


title: SpColCtrl_rep2a1572


title: SpColCtrl_rep2a1573


title: SpColCtrl_rep2a1567


title: SpColCtrl_rep2a1568


title: SpColCtrl_rep2a1570


title: SpColCtrl_rep2a1574


title: SpColCtrl_rep2a1591


title: SpColCtrl_rep2a1564


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title: SpColCtrl_rep2a1560


title: SpColCtrl_rep2a1561


title: SpColCtrl_rep2a1566


title: SpColCtrl_rep2a1558


title: SpColCtrl_rep2a2123


title: SpColCtrl_rep2a1553


title: SpColCtrl_rep2a1554


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title: SpColCtrl_rep2a1556


title: SpColCtrl_rep2a1557


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title: SpColCtrl_rep2a1550


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title: SpColCtrl_rep2a1546


title: SpColCtrl_rep2a1545


title: SpColCtrl_rep2a1540


title: SpColCtrl_rep2a1539


title: SpColCtrl_rep2a1537


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title: SpColCtrl_rep2a1538


title: SpColCtrl_rep2a1536


title: SpColCtrl_rep2a1534


title: SpColCtrl_rep2a1535


title: SpColCtrl_rep2a1533


title: SpColCtrl_rep2a1532


title: SpColCtrl_rep2a1531


title: SpColCtrl_rep2a1530


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title: SpColCtrl_rep2a1512


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title: SpColCtrl_rep2a1495


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title: SpColCtrl_rep2a1477


title: SpColCtrl_rep2a1476


title: SpColCtrl_rep2a1474


title: SpColCtrl_rep2a1473


title: SpColCtrl_rep2a1467


title: SpColCtrl_rep2a1469


title: SpColCtrl_rep2a1471


title: SpColCtrl_rep2a1470


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title: SpColCtrl_rep2a1455


title: SpColCtrl_rep2a1458


title: SpColCtrl_rep2a1452


title: SpColCtrl_rep2a1454


title: SpColCtrl_rep2a1457


title: SpColCtrl_rep2a1451


title: SpColCtrl_rep2a1450


title: SpColCtrl_rep2a1449


title: SpColCtrl_rep2a1447


title: SpColCtrl_rep2a1446


title: SpColCtrl_rep2a1445


title: SpColCtrl_rep2a1453


title: SpColCtrl_rep2a1448


title: SpColCtrl_rep2a1443


title: SpColCtrl_rep2a1444


title: SpColCtrl_rep2a1441


title: SpColCtrl_rep2a1442


title: SpColCtrl_rep2a1437


title: SpColCtrl_rep2a1439


title: SpColCtrl_rep2a1435


title: SpColCtrl_rep2a1432


title: SpColCtrl_rep2a1440


title: SpColCtrl_rep2a1436


title: SpColCtrl_rep2a1434


title: SpColCtrl_rep2a1431


title: SpColCtrl_rep2a1429


title: SpColCtrl_rep2a1428


title: SpColCtrl_rep2a1433


title: SpColCtrl_rep2a1430


title: SpColCtrl_rep2a1425


title: SpColCtrl_rep2a1438


title: SpColCtrl_rep2a1456


title: SpColCtrl_rep2a1426


title: SpColCtrl_rep2a1422


title: SpColCtrl_rep2a1421


title: SpColCtrl_rep2a1420


title: SpColCtrl_rep2a1418


title: SpColCtrl_rep2a1419


title: SpColCtrl_rep2a1415


title: SpColCtrl_rep2a1414


title: SpColCtrl_rep2a1416


title: SpColCtrl_rep2a1413


title: SpColCtrl_rep2a1472


title: SpColCtrl_rep2a1412


title: SpColCtrl_rep2a1411


title: SpColCtrl_rep2a1417


title: SpColCtrl_rep2a1410


title: SpColCtrl_rep2a1406


title: SpColCtrl_rep2a1407


title: SpColCtrl_rep2a1409


title: SpColCtrl_rep2a1408


title: SpColCtrl_rep2a1403


title: SpColCtrl_rep2a1405


title: SpColCtrl_rep2a1404


title: SpColCtrl_rep2a1400


title: SpColCtrl_rep2a1399


title: SpColCtrl_rep2a1427


title: SpColCtrl_rep2a1401


title: SpColCtrl_rep2a1424


title: SpColCtrl_rep2a1395


title: SpColCtrl_rep2a1396


title: SpColCtrl_rep2a1398


title: SpColCtrl_rep2a1394


title: SpColCtrl_rep2a1423


title: SpColCtrl_rep2a1390


title: SpColCtrl_rep2a1393


title: SpColCtrl_rep2a1392


title: SpColCtrl_rep2a1387


title: SpColCtrl_rep2a1389


title: SpColCtrl_rep2a1385


title: SpColCtrl_rep2a1388


title: SpColCtrl_rep2a1402


title: SpColCtrl_rep2a1384


title: SpColCtrl_rep2a1381


title: SpColCtrl_rep2a1397


title: SpColCtrl_rep2a1382


title: SpColCtrl_rep2a1380


title: SpColCtrl_rep2a1379


title: SpColCtrl_rep2a1391


title: SpColCtrl_rep2a1371


title: SpColCtrl_rep2a1370


title: SpColCtrl_rep2a1373


title: SpColCtrl_rep2a1377


title: SpColCtrl_rep2a1374


title: SpColCtrl_rep2a1376


title: SpColCtrl_rep2a1366


title: SpColCtrl_rep2a1386


title: SpColCtrl_rep2a1367


title: SpColCtrl_rep2a1365


title: SpColCtrl_rep2a1359


title: SpColCtrl_rep2a1383


title: SpColCtrl_rep2a1361


title: SpColCtrl_rep2a1360


title: SpColCtrl_rep2a1363


title: SpColCtrl_rep2a1368


title: SpColCtrl_rep2a1378


title: SpColCtrl_rep2a1375


title: SpColCtrl_rep2a1355


title: SpColCtrl_rep2a1353


title: SpColCtrl_rep2a1357


title: SpColCtrl_rep2a1354


title: SpColCtrl_rep2a1372


title: SpColCtrl_rep2a1348


title: SpColCtrl_rep2a1352


title: SpColCtrl_rep2a1358


title: SpColCtrl_rep2a1349


title: SpColCtrl_rep2a1345


title: SpColCtrl_rep2a1351


title: SpColCtrl_rep2a1369


title: SpColCtrl_rep2a1344


title: SpColCtrl_rep2a1362


title: SpColCtrl_rep2a1347


title: SpColCtrl_rep2a1339


title: SpColCtrl_rep2a1346


title: SpColCtrl_rep2a1350


title: SpColCtrl_rep2a1356


title: SpColCtrl_rep2a1335


title: SpColCtrl_rep2a1343


title: SpColCtrl_rep2a1337


title: SpColCtrl_rep2a1338


title: SpColCtrl_rep2a1342


title: SpColCtrl_rep2a1341


title: SpColCtrl_rep2a1322


title: SpColCtrl_rep2a1340


title: SpColCtrl_rep2a1336


title: SpColCtrl_rep2a1333


title: SpColCtrl_rep2a1325


title: SpColCtrl_rep2a1317


title: SpColCtrl_rep2a1328


title: SpColCtrl_rep2a1315


title: SpColCtrl_rep2a1313


title: SpColCtrl_rep2a1331


title: SpColCtrl_rep2a1332


title: SpColCtrl_rep2a1330


title: SpColCtrl_rep2a1312


title: SpColCtrl_rep2a1364


title: SpColCtrl_rep2a1323


title: SpColCtrl_rep2a1321


title: SpColCtrl_rep2a1324


title: SpColCtrl_rep2a1320


title: SpColCtrl_rep2a1334


title: SpColCtrl_rep2a1318


title: SpColCtrl_rep2a1316


title: SpColCtrl_rep2a1314


title: SpColCtrl_rep2a1302