title: colon, control diet, age 28 months, rep17

city: Wageningen

first: Guido

last: Hooiveld

address: HELIX, Stippeneng 4

department: Div. Human Nutrition & Health

institute: Wageningen University

hyb_protocol: Hybridization and washing of the Affymetrix GeneChip Mouse Gene 1.1 ST peg arrays were performed on a GeneTitan Instrument (Affymetrix, Santa Clara, CA) according to the manufacturer's recommendations.

scan_protocol: Arrays were scanned on an Affymetrix GeneTitan instrument (Affymetrix, Santa Clara, CA).

data_processing: Expression estimates were calculated applying the RMA algorithm in the Bioconductor library 'Oligo' (v1.40.1).

source_name: scraping from colonic mucosa and submucosa, control diet, age 28 months

label_protocol: Purified total RNA (100ng per sample) was labeled with the Whole-Transcript Sense Target Assay (Affymetrix, Santa Clara, CA, USA; P/N 900652).

growth_protocol: The design of the study and experimental procedures have been described in detail previously (PMID: 25504628).  Briefly, male C57BL/6J mice (age: 7 weeks) were purchased from Janvier (Cedex, France) and were housed in pairs of two in the light and temperature (20C)-controlled animal facility of Wageningen University (12-hour light/dark cycle, lights on at 04.00). The mice received standard AIN-93G (Research Diet Services, Wijk bij Duurstede, The Netherlands) for 2 weeks upon arrival. At the age of 9 weeks, mice were individually housed and randomized to different dietary regimens. For the current analyses, we considered mice that were lifelong fed a semi-synthetic control diet consisting of 10E% of fat (C; control), a calorie restriction (CR) diet without malnutrition (30% energy reduction compared to the C diet) or a medium fat (MF) diet consisting of 25E% of fat. All diets, except the CR diet, were fed ad libitum. Mice sacrificed at 6 or 28 months of age were considered in order to differentiate between effects at young and old age. To study the molecular flexibility and dynamics after receiving a strict CR diet for prolonged time, a diet switch was included in this study. A subgroup of mice on CR was switched to the MF diet at the age of 24 months until sacrifice at 28 months.

extract_protocol: Total RNA was isolated from the scrapings using TRIzol Reagent according to the manufacturer’s instructions (Invitrogen, Breda, The Netherlands). Isolated RNA was purified using RNeasy Micro columns (Qiagen, Venlo, The Netherlands) and total RNA yield (Nanodrop ND-1000, Nanodrop Products, Maarssen, The Netherlands) and RNA integrity (Agilent 2100 Bioanalyzer, Agilent Technologies, Amsterdam, The Netherlands) were assessed. RNA was judged as suitable for array hybridization only if samples exhibited intact bands corresponding to the 18S and 28S ribosomal RNA subunits, and displayed no chromosomal peaks or RNA degradation products (RNA Integrity Number > 8.0).

treatment_protocol: Prior to sacrifice, each mouse was first fasted for 4 hours after which they received an intragastric gavage of 400ul of 0.5% carboxymethyl cellulose, then fasted again for another 6 hours. The animals in CR diet group were fed with half of the portion they normally received 15 min before the first fasting. During the sacrifice, after sedation with a mixture of isoflurane (1.5%), nitrous oxide (70%) and oxygen (30%), tissue was snap-frozen and stored at -80C until further analysis.

tag: Sex

value: male

tag: mouseid

tag: diet group

value: C

tag: diet group (full name)

value: control

tag: age at sacrifice (months)

tag: diet switch at the age of 24m

value: no

value: colon


title: Y-361-B

status: Public on Mar 06 2018

type: SRA

city: Mainz

first: Matteo

middle: Antoine

last: Negroni

address: Joh.-von-Müller-Weg 6 Room 02-239

department: Institute of Organismic and Molecular Evolution

country: Germany

institute: Johannes Gutenberg, University, Mainz

description:
Table_S3_Differentially_expressed_within_brain_between_age_classes.xlsx
Table_S4_Differentially_expressed_within_fat_body_between_age_classes.xlsx
Table_S8_Differentially_expressed_within_young_between_tissues.xlsx
Table_S9_Differentially_expressed_within_old_between_tissues.xlsx
Table-S6.csv

library_source: transcriptomic

data_processing:
FastQC-v0.11.5; default parameters
Trimmomatic-v0.36; default parameters
Trinityrnaseq-Trinity-v2.4.0; command: Trinity --seqType fq --max_memory 200G --left 03_R1_trimP.fastq.gz --right 03_R2_trimP.fastq.gz --CPU 32 --min_contig_length 300 --output Trinity_assembly --full_cleanup --no_bowtie
RSEM-v1.3.0; command: ./align_and_estimate_abundance.pl --transcripts Trinity_assembly.fasta --seqType fq --left sample-1-paired-forward-F --right sample-1-paired-reverse-F --thread_count 10 --est_method RSEM --prep_reference --aln_method bowtie2 --output_dir RSEM-output-sample-1
R package Deseq2-v1.2.10
Genome_build: de novo transcriptome assembly
Supplementary_files_format_and_content: Table_S3_Differentially_expressed_within_brain_between_age_classes.xlsx; Table_S4_Differentially_expressed_within_fat_body_between_age_classes.xlsx; Table-S6.csv (normalized read count output from RSEM); Table_S8_Differentially_expressed_within_young_between_tissues.xlsx; Table_S9_Differentially_expressed_within_old_between_tissues.xlsx ; Trinity_assembly.fasta

molecule: total RNA

organism: Temnothorax rugatulus

source_name: brain

extract_protocol:
Each individual was killed by decapitation, and brain, fat body were immediately dissected on ice. Tissue dissection took less than 10 minutes for each individual. Brain and fat body were separately crushed in 50 μL of TRIZOL for storage at -20°C before RNA extraction. RNA was extracted from fat body and brain samples separately using the RNAeasy mini extraction kit (Qiagen)
Libraries were constructed by BGI (Hongkong, China) immediately prior to sequencing

tag: individual

tag: age

value: Young

tag: specimen type

value: Queen

tag: tissue

value: brain


title: colon, control diet, age 28 months, rep05

status: Public on Mar 27 2018

type: RNA

country: Netherlands

label: biotin

organism: Mus musculus

tag: strain


title: colon, control diet, age 6 months, rep09

source_name: scraping from colonic mucosa and submucosa, control diet, age 6 months


title: colon, control diet, age 28 months, rep01


title: colon, control diet, age 6 months, rep07


title: colon, control diet, age 28 months, rep19


title: colon, control diet, age 6 months, rep03


title: colon, control diet, age 6 months, rep11


title: colon, control diet, age 28 months, rep09


title: colon, control diet, age 28 months, rep21


title: colon, control diet, age 28 months, rep03


title: Y-333-F

source_name: fat body

value: fat body


title: Y-134-F


title: Y-594-F


title: Y-333-B


title: Y-102-F


title: Y-134-B

description:
Table_S3_Differentially_expressed_within_brain_between_age_classes.xlsx
Table_S4_Differentially_expressed_within_fat_body_between_age_classes.xlsx
Table_S8_Differentially_expressed_within_young_between_tissues.xlsx
Table_S9_Differentially_expressed_within_old_between_tissues.xlsx
Table-S6.csv

Before trimming the raw reads files, for sample Y-134-B:
Files FCH7F5CBBXX_L8_HKQUEnywEAAORACPEI-215_1.fq.gz (SRR6806701, md5sum: 0d6dd16fa11ce8429bae95b53a25e6a2) and FCH7F5CBBXX_L7_HKQUEnywEAAORACPEI-215_1.fq.gz (SRR6806702, md5sum: cfd9f95610dbeff7955f5efcaea099c8) were concatenated into a single one, as well as files FCH7F5CBBXX_L8_HKQUEnywEAAORACPEI-215_2.fq.gz (SRR6806701, md5sum: ecb79edbfbbfd7e5dab7b8939bd75017) and FCH7F5CBBXX_L7_HKQUEnywEAAORACPEI-215_2.fq.gz (SRR6806702, md5sum: 67c2175dfd252705a703bb16ba86d15d) into a single one.


title: Y-102-B


title: Y-453-B


title: O-829-B

value: Old


title: O-482-B


title: O-482-F


title: O-603-F


title: O-603-B


title: O-829-F


title: O-796-B


title: O-469-B


title: d-1d-TRT-psc-2_H7JWVCCXY_L3

status: Public on Mar 31 2018

city: Wuhan

first: Thuy Nhien

last: Tran Thi

state: Hubei

address: Shizishan

country: China

institute: Huazhong Agricultural University

data_processing:
The quality of RNA sequence data was controlled using FastQC software and trimmed using the Trimmomatic tool
The trimmed adapters and purified sequences were mapped and identified based on Ensembl Sus scrofa 11.1.90 set GTF annotation genome utilizing HISAT2
Stringtie was used to estimate gene expression and transcript levels via FPKM (fragments per kilobase of transcript per million mapped fragments) approach
Genome_build: Ensembl Sus scrofa 11.1.90
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each sample

organism: Sus scrofa

source_name: Muscle satellite cells

growth_protocol: Porcine satellite cells (PSCs) were isolated from the hindlimb muscle of 5-days-old male piglet.After isolating, PSCs were seeded at a density of (specific density/cm2), in 6-well plates pre-coated with gel, and incubated at 37°C under 5%CO2overnight with growth medium

extract_protocol:
At each time point, the poisoned effect of PSCs was harvested by TRIzol 0.25%.
A  total  amount  of  1.5μg  RNA  per  sample  was  used  as  input  material  for  the  RNA  sample preparations.  Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample.

treatment_protocol: After 24h of culturing, porcine satellite cells  were treated with 10 µg/mL of poly (I:C) within 12h and untreated PSCs as control. Next, the cells were washed once with PBS and entered into differentiation stages. The triplication of each sample at 4-time points, including0h and 12h of proliferation stage, at 24h and 48h of differentiation stage, was collected from poly (I:C) stimulation.

tag: cell type

value: Muscle satellite cells

tag: developmental stage

value: differentiation

tag: time

value: 24 hours

tag: untreated and treated poly (i


title: O-426-F


title: O-426-B


title: O-269-F


title: O-469-F


title: O-269-B


title: p-12h-TRT-psc-1_H7JN2CCXY_L2

value: proliferation

value: 12 hours


title: O-189-B


title: O-189-F


title: O-796-F


title: LON0146_H1N1_T3

status: Public on Mar 03 2018

city: London

first: Long

last: Hoang

address: Dovehouse Street

department: Genomic Medicine

country: United Kingdom

institute: Imperial College London

description:
7509336056_H
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)

hyb_protocol: Illumina

scan_protocol: Illumina iScan

data_processing: GenomeStudio (Illumina). Raw microarray data were processed using GeneSpring GX version 12.5 (Agilent Technologies). Following background subtraction, each probe was attributed a flag to denote its signal intensity detection P value. Filtering on flags removed probe sets that did not result in a ‘present’ call in at least 10% of the samples, where the ‘present’ lower cut-off = 0.99. 594 Signal values were then set to a threshold level of 10, log2 transformed, and per-chip normalised using a 75th percentile-shift algorithm. Each gene was normalised by dividing each mRNA transcript by the median intensity of all samples. Statistical analysis was performed after these steps had been performed.

label: Biotin

organism: Homo sapiens

source_name: Whole blood

label_protocol: Biotinylated, amplified antisense complementary RNA (cRNA) targets were prepared from 200-250 ng of globin-reduced RNA using the Illumina CustomPrep RNA amplification kit (Applied Biosystems/Ambion)

treatment_protocol: stored at -80°C

tag: subject id

tag: timepoint

tag: day of illness

value: Male

tag: ethnicity

value: White

value: Yes


title: LON0177_H1N1_T1

description:
7509336056_G
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)

tag: Sex

value: Asian


title: 2027_HC_HC

description:
7509336056_K
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)

value: HC

value: Female


title: LON0102_H1N1_T3

description:
7509336054_J
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)

value: Excluded


title: 2179_HC_HC

description:
7509336052_H
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0113_H1N1_T2

description:
7509336056_I
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)

value: Black

value: No


title: ROY0011_H1N1_T3

description:
7509336056_B
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0022_HC_HC

description:
7509336056_L
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0175_H1N1_T2

description:
7509336056_D
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0160_H1N1_T2

description:
7509336051_K
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2028_HC_HC

description:
7509336052_B
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0167_H1N1_T1

description:
7509336052_J
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0179_H1N1_T3

description:
7509336052_G
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0092_B_T1

description:
7509336056_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)

value: B


title: ROY0020_H1N1_T2

description:
7509336054_E
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0009_H1N1_T1

description:
7509336054_D
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2088_HC_HC

description:
7509336054_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0077_H1N1_T2

description:
7509336056_F
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0176_H1N1_T1

description:
7509336054_B
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0136_H1N1_T1

description:
7509336054_H
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0087_H1N1_T2

description:
7509336052_E
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0031_H1N1_T3

description:
7509336052_D
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0019_H1N1_T2

description:
7509336054_F
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2087_HC_HC

description:
7509336052_A
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0153_H1N1_T1

description:
7509336054_A
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0027_HC_HC

description:
7509336052_I
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0132_H1N1_T2

description:
7509336054_I
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0015_HC_HC

description:
7509336051_I
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0076_H1N1_T3

description:
7509336052_F
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0134_H1N1_T1

description:
7509336051_H
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0139_H1N1_T1

description:
7509336052_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2168_HC_HC

description:
7509336051_F
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0094_H1N1_T2

description:
7509336051_B
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2039_HC_HC

description:
7509336050_G
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0096_H1N1_T1

description:
7509336050_E
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)

value: Other


title: 2170_HC_HC

description:
7509336050_J
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2035_HC_HC

description:
7509336050_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0094_H1N1_T1

description:
7509336049_B
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0016_H1N1_T2

description:
7509336049_A
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0097_H1N1_T2

description:
7509336049_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0177_H1N1_T2

description:
7509336046_L
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2146_HC_HC

description:
7509336046_J
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0173_H1N1_T1

description:
7509336049_J
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0016_H1N1_T1

description:
7509336050_H
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2067_HC_HC

description:
7509336051_A
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0124_B_T1

description:
7509336051_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0092_B_T2

description:
7509336046_I
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0163_B_T3

description:
7509336046_H
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0091_H1N1_T2

description:
7509336051_D
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0127_H1N1_T1

description:
7509336050_B
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 10170_HC_HC

description:
7509336050_D
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0010_H1N1_T1

description:
7509336049_F
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0084_H1N1_T2

description:
7509336050_A
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0147_H1N1_T3

description:
7509336049_L
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0102_H1N1_T2

description:
7509336046_F
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2041_HC_HC

description:
7509336050_I
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0001_HC_HC

description:
7509336049_G
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0026_H1N1_T1

description:
7509336046_E
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0178_B_T2

description:
7509336046_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0076_H1N1_T1

description:
7509336049_D
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0016_HC_HC

description:
7509336046_D
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2116_HC_HC

description:
7509336046_K
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0128_H1N1_T1

description:
7509336046_B
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0122_H1N1_T2

description:
7509336046_A
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0126_H1N1_T2

description:
7509336045_K
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0126_H1N1_T1

description:
7509336045_J
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2149_HC_HC

description:
7509336044_L
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0109_H1N1_T1

description:
7509336044_K
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0138_H1N1_T2

description:
7509336045_D
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2064_HC_HC

description:
7509336044_I
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0186_H1N1_T1

description:
7509336045_I
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0091_H1N1_T1

description:
7509336044_G
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0018_HC_HC

description:
7509336044_F
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0156_H1N1_T2

description:
7509336044_D
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 10173_HC_HC

description:
7509336045_A
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2188_HC_HC

description:
7509336044_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0014_H1N1_T2

description:
7509336045_H
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0119_H1N1_T1

description:
7509336045_G
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2089_HC_HC

description:
7509336044_A
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2163_HC_HC

description:
7509336044_B
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0088_H3N2_T1

description:
7509336045_F
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0150_H1N1_T1

description:
7509336042_I
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0151_B_T2

description:
7509336042_H
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2118_HC_HC

description:
7509336042_E
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0084_H1N1_T1

description:
7509336042_F
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0011_HC_HC

description:
7509336042_G
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0098_H1N1_T2

description:
7509336042_D
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0146_H1N1_T2

description:
7509336042_J
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0078_H1N1_T1

description:
7509336042_A
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2085_HC_HC

description:
7509336041_L
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0009_HC_HC

description:
7509336041_F
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0104_H1N1_T1

description:
7509336041_G
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0019_HC_HC

description:
7509336041_I
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0018_H1N1_T3

description:
7509336041_K
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0025_HC_HC

description:
7509336041_H
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0021_H1N1_T2

description:
7509336041_D
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0184_H3N2_T3

description:
7509336040_G
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2128_HC_HC

description:
7509336041_B
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0179_H1N1_T2

description:
7509336040_I
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2205_HC_HC

description:
7509336040_K
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0019_H1N1_T1

description:
7509336039_F
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0008_H1N1_T1

description:
7509336040_J
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2120_HC_HC

description:
7509336040_F
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 10174_HC_HC

description:
7509336040_B
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0146_H1N1_T1

description:
7509336041_A
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0127_H1N1_T3

description:
7509336040_H
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2138_HC_HC

description:
7509336040_E
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2051_HC_HC

description:
7509336039_J
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2171_HC_HC

description:
7509336040_L
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0031_H1N1_T1

description:
7509336040_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2175_HC_HC

description:
7509336039_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0137_H1N1_T2

description:
7509336039_H
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0143_H1N1_T3

description:
7509336039_D
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2155_HC_HC

description:
7509336039_K
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0034_H1N1_T2

description:
7509336039_E
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0025_H1N1_T2

description:
7509336039_L
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0029_HC_HC

description:
7509336038_L
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2194_HC_HC

description:
7509336039_A
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0089_H1N1_T2

description:
7509336039_B
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0108_H1N1_T1

description:
7509336038_K
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0111_H1N1_T1

description:
7509336038_E
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0184_H3N2_T1

description:
7509336038_F
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2182_HC_HC

description:
7509336038_H
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2086_HC_HC

description:
7509336038_G
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0105_H1N1_T1

description:
7509336038_I
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0075_H1N1_T3

description:
7509336037_H
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0012_H1N1_T1

description:
7509336038_A
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0095_H1N1_T3

description:
7509336037_J
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 10154_HC_HC

description:
7509336037_I
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0028_HC_HC

description:
7509336037_D
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0129_H1N1_T2

description:
7509336037_K
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2191_HC_HC

description:
7509336036_D
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 10172_HC_HC

description:
7509336036_L
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2151_HC_HC

description:
7509336037_F
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0101_B_T1

description:
7509336036_J
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 10167_HC_HC

description:
7509336036_K
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0159_B_T2

description:
7509336036_F
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0082_H1N1_T1

description:
7509336037_E
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2104_HC_HC

description:
7509336038_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0179_H1N1_T1

description:
7509336036_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0036_H1N1_T2

description:
7509336037_L
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0138_H1N1_T1

description:
7509336037_B
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0082_H1N1_T2

description:
7509336038_B
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0186_H1N1_T2

description:
7509336036_G
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0090_H1N1_T1

description:
7509336035_K
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0024_HC_HC

description:
7509336037_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0079_H1N1_T2

description:
7509336037_A
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2177_HC_HC

description:
7509336035_J
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0019_H1N1_T3

description:
7509336038_D
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2042_HC_HC

description:
7509336035_G
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0077_H1N1_T3

description:
7509336035_I
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0030_B_T1

description:
7509336035_E
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2127_HC_HC

description:
7509336035_D
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0150_H1N1_T3

description:
7509336035_F
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0151_B_T1

description:
7509336035_H
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0083_H1N1_T2

description:
7509336035_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0023_HC_HC

description:
7509336035_A
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0013_HC_HC

description:
7509336034_B
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0011_H1N1_T2

description:
7509336033_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2117_HC_HC

description:
7509336032_L
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0173_H1N1_T2

description:
7509336034_H
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2167_HC_HC

description:
7509336033_L
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0093_B_T1

description:
7509336032_K
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0145_H1N1_T2

description:
7509336033_K
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0169_H1N1_T1

description:
7509336033_B
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0080_H1N1_T1

description:
7509336033_J
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0097_H1N1_T1

description:
7509336034_G
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0176_H1N1_T3

description:
7509336033_F
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0014_H1N1_T1

description:
7509336032_G
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0024_H1N1_T1

description:
7509336033_D
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2181_HC_HC

description:
7509336034_E
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0158_H1N1_T2

description:
7509336034_K
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0157_H1N1_T1

description:
7509336034_J
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0159_B_T3

description:
7509336033_G
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2107_HC_HC

description:
7509336033_E
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0079_H1N1_T1

description:
7509336033_I
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2193_HC_HC

description:
7509336033_H
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0102_H1N1_T1

description:
7509336032_F
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0175_H1N1_T1

description:
7509336034_L
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0165_B_T2

description:
7509336032_I
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0032_HC_HC

description:
7509336034_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 10163_HC_HC

description:
7509336032_D
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0004_HC_HC

description:
7509336032_J
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 10144_HC_HC

description:
7509336032_B
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0114_H1N1_T1

description:
7509336032_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0109_H1N1_T2

description:
7509336032_E
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0147_H1N1_T1

description:
7509336032_A
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0152_A_T2

description:
7509336031_G
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)

value: A


title: LON0156_H1N1_T3

description:
7509336031_A
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0090_H1N1_T2

description:
7509336031_H
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0075_H1N1_T2

description:
7509336030_D
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0016_H1N1_T3

description:
7509336030_L
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2185_HC_HC

description:
7509336029_K
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0089_H1N1_T1

description:
7509336031_L
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0014_HC_HC

description:
7509336031_K
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0180_H1N1_T1

description:
7509336030_I
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0077_H1N1_T1

description:
7509336030_K
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0114_H1N1_T2

description:
7509336031_J
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0034_HC_HC

description:
7509336030_G
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 10146_HC_HC

description:
7509336030_B
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0018_H1N1_T2

description:
7509336029_D
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0095_H1N1_T1

description:
7509336030_F
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0033_HC_HC

description:
7509336031_D
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2141_HC_HC

description:
7509336031_B
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0035_HC_HC

description:
7509336029_L
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0108_H1N1_T3

description:
7509336029_H
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0178_B_T1

description:
7509336030_H
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0024_H1N1_T3

description:
7509336030_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0163_B_T2

description:
7509336031_E
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0149_B_T3

description:
7509336030_A
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0112_H1N1_T1

description:
7509336029_I
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0159_B_T1

description:
7509336029_G
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2126_HC_HC

description:
7509336031_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2162_HC_HC

description:
7509336029_B
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0165_B_T1

description:
7509336029_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0118_H1N1_T1

description:
7509336029_F
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0128_H1N1_T2

description:
7509336029_A
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2078_HC_HC

description:
7509336028_I
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0173_H1N1_T3

description:
7509336028_H
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2159_HC_HC

description:
7509336028_D
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2062_HC_HC

description:
7509336027_L
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2173_HC_HC

description:
7509336027_J
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0121_H1N1_T1

description:
7509336027_H
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0017_H1N1_T1

description:
7509336028_L
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0148_H1N1_T1

description:
7509336027_K
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0085_H1N1_T1

description:
7509336028_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0133_H1N1_T2

description:
7509336028_A
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0008_HC_HC

description:
7509336027_A
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2180_HC_HC

description:
7509336027_B
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0177_H1N1_T3

description:
7509336027_I
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0075_H1N1_T1

description:
7509336026_I
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0176_H1N1_T2

description:
7509336027_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0012_H1N1_T3

description:
7509336026_A
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0158_H1N1_T1

description:
7509336025_L
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0156_H1N1_T1

description:
7509336026_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0100_H1N1_T3

description:
7509336026_G
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0086_H1N1_T1

description:
7509336028_G
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2084_HC_HC

description:
7509336026_K
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0139_H1N1_T3

description:
7509336027_D
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0157_H1N1_T3

description:
7509336026_J
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0078_H1N1_T3

description:
7509336027_F
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0007_HC_HC

description:
7509336027_G
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2029_HC_HC

description:
7509336026_D
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2119_HC_HC

description:
7509336028_K
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2082_HC_HC

description:
7509336028_E
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0017_HC_HC

description:
7509336026_B
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0088_H3N2_T2

description:
7509336026_F
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0086_H1N1_T2

description:
7509336025_H
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0021_H1N1_T1

description:
7509336025_J
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2125_HC_HC

description:
7509336025_F
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0023_H1N1_T1

description:
7509336025_G
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0165_B_T3

description:
7509336025_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0183_B_T1

description:
7509336024_K
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0098_H1N1_T1

description:
7509336025_D
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2156_HC_HC

description:
7509336024_I
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0122_H1N1_T1

description:
7509336025_A
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0015_H1N1_T2

description:
7509336025_B
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0031_H1N1_T2

description:
7509336024_H
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0020_H1N1_T1

description:
7509336024_F
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0154_H1N1_T1

description:
7509336023_K
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0087_H1N1_T1

description:
7509336024_D
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0015_H1N1_T1

description:
7509336024_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0009_H1N1_T3

description:
7509336024_J
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 10140/2_HC_HC

description:
7509336024_E
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0034_H1N1_T1

description:
7509336023_J
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0116_H1N1_T1

description:
7509336025_E
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0160_H1N1_T3

description:
7509336023_E
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0160_H1N1_T1

description:
7509336021_H
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0135_H1N1_T1

description:
7509336021_L
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0008_H1N1_T2

description:
7509336023_B
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2172_HC_HC

description:
7509336024_G
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0006_HC_HC

description:
7509336021_J
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0140_H1N1_T1

description:
7509336021_F
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0025_H1N1_T1

description:
7509336021_K
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0137_H1N1_T1

description:
7509336023_A
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2135_HC_HC

description:
7509336023_I
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0133_H1N1_T1

description:
7509336021_E
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0118_H1N1_T3

description:
7509336023_H
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0036_HC_HC

description:
7509336021_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0036_H1N1_T1

description:
7509336021_B
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0003_HC_HC

description:
7509336021_A
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2183_HC_HC

description:
7509336020_L
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 10157_HC_HC

description:
7509336020_J
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0010_H1N1_T3

description:
7509336020_G
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0124_B_T3

description:
7509336020_K
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0026_H1N1_T3

description:
7509336020_A
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 10171_HC_HC

description:
7509336020_F
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0021_H1N1_T3

description:
7509336020_H
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 10169_HC_HC

description:
7509336019_H
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0125_H1N1_T2

description:
7509336020_E
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0026_HC_HC

description:
7509336019_A
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0010_HC_HC

description:
7509336019_G
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2147_HC_HC

description:
7509336019_B
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0134_H1N1_T3

description:
7509336019_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0113_H1N1_T3

description:
7509336019_E
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0145_H1N1_T1

description:
7509336018_L
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0025_H1N1_T3

description:
7509336020_D
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 10165_HC_HC

description:
7509336020_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 10152_HC_HC

description:
7509336018_K
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0008_H1N1_T3

description:
7509336019_K
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2174_HC_HC

description:
7509336018_H
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0090_H1N1_T3

description:
7509336019_D
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0117_H1N1_T1

description:
7509336019_I
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 10164_HC_HC

description:
7509336018_J
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2169_HC_HC

description:
7509336018_G
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0031_HC_HC

description:
7509336018_F
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0020_H1N1_T3

description:
7509336018_E
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0167_H1N1_T3

description:
7509336018_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0136_H1N1_T3

description:
7509336017_G
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2142_HC_HC

description:
7509336017_I
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0024_H1N1_T2

description:
7509336018_D
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0123_H1N1_T1

description:
7509336017_D
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0153_H1N1_T2

description:
7509336017_J
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0018_H1N1_T1

description:
7509336017_E
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2137_HC_HC

description:
7509336018_A
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0011_H1N1_T1

description:
7509336017_B
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0149_B_T1

description:
7509336016_I
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0080_H1N1_T2

description:
7509336017_F
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 10147_HC_HC

description:
7509336016_E
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2189_HC_HC

description:
7509336017_H
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0132_H1N1_T1

description:
7509336017_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0083_H1N1_T1

description:
7509336015_F
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0152_A_T1

description:
7509336016_B
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2197_HC_HC

description:
7509336015_G
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0147_H1N1_T2

description:
7509336015_L
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0108_H1N1_T2

description:
7509336015_K
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0009_H1N1_T2

description:
7509336016_A
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0100_H1N1_T1

description:
7509336015_J
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0005_HC_HC

description:
7509336016_F
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 10141/2_HC_HC

description:
7509336016_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2050_HC_HC

description:
7509336016_G
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0097_H1N1_T3

description:
7509336015_D
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2043_HC_HC

description:
7509336016_H
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2108_HC_HC

description:
7509336015_E
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2150_HC_HC

description:
7509336016_K
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: ROY0023_H1N1_T2

description:
7509336015_B
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 10156_HC_HC

description:
7509336011_K
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0099_H1N1_T1

description:
7509336011_L
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0002_HC_HC

description:
7509336011_G
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0143_H1N1_T1

description:
7509336011_J
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0030_HC_HC

description:
7509336011_A
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0134_H1N1_T2

description:
7509336011_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0163_B_T1

description:
7509336011_I
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0020_HC_HC

description:
7509336011_F
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 10159_HC_HC

description:
7509336015_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0125_H1N1_T1

description:
7509336004_K
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 10160_HC_HC

description:
7509336004_C
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HC0021_HC_HC

description:
7509336004_H
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0129_H1N1_T1

description:
7509336011_E
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0113_H1N1_T1

description:
7509336004_G
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2176_HC_HC

description:
7509336004_J
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0104_H1N1_T3

description:
7509336003_H
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0157_H1N1_T2

description:
7509336004_F
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: CFHBE RNA-seq rep2

status: Public on Feb 28 2018

city: Cleveland

first: Ann

last: Harris

state: OH

address: 10900 Euclid Avenue

country: USA

institute: Case Western Reserve University Medical School

description: processed data: gene_exp.diff

data_processing:
Bowtie used for alignment after removing first 20 bases of each read.
Sequences are mapped to hg19 reference genome with parameters:  -q -m 1 -l 20 -n 3 --best --strata -p 10.
Peak calls for the DNase-seq data were performed using MACS2.
RNA samples were analyzed with TopHat and Cufflinks.
Genome_build: hg19
Supplementary_files_format_and_content: BED file containing the locations of DNase hypersensitive sites (DNase-seq) and a tab-delimited table of cufflinks isoform-level FPKM expression data

source_name: CFHBE RNA-seq

growth_protocol: Cells were grown as decribed in Gillen et al. 2017. In review.

extract_protocol:
TruSeq RNA Sample Preparation Kit v2
genomic DNA is digested with different concentrations of DNaseI (NEB) and purified for library construction. Total RNA is extracted by Trizol (HBE) or RNeasy (HTE)
DNase-seq libraries were prepared as described in Bischof et al. Thorax. 67:385-391. 2012. RNA seq libraries prepared by standard Illumina protocols.

tag: donor status

value: donors with Cystic Fibrosis

value: primary human CF bronchial epithelial cells


title: LON0148_H1N1_T2

description:
7509336003_J
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: HBE RNA-seq rep2

description: processed data: gene_exp.diff

source_name: HBE RNA-seq

value: healthy donor

value: primary human bronchial epithelial cells


title: HTE RNA-seq rep3

description: processed data: gene_exp.diff

source_name: HTE RNA-seq

extract_protocol:
TruSeq Stranded Total RNA Library Prep kit with Ribo-Zero Gold
genomic DNA is digested with different concentrations of DNaseI (NEB) and purified for library construction. Total RNA is extracted by Trizol (HBE) or RNeasy (HTE)
DNase-seq libraries were prepared as described in Bischof et al. Thorax. 67:385-391. 2012. RNA seq libraries prepared by standard Illumina protocols.

value: primary human tracheal epithelial cells


title: HBE RNA-seq rep1

description: processed data: gene_exp.diff


title: HBE RNA-seq rep4

description: processed data: gene_exp.diff


title: HBE RNA-seq rep3

description: processed data: gene_exp.diff


title: CFHBE DNase-seq rep2

description: processed data: DNase_seq_peaks.bed

library_source: genomic

molecule: genomic DNA

source_name: CFHBE DNase-seq

extract_protocol:
genomic DNA is digested with different concentrations of DNaseI (NEB) and purified for library construction. DNase-seq libraries were prepared as described in Bischof et al. Thorax. 67:385-391. 2012
genomic DNA is digested with different concentrations of DNaseI (NEB) and purified for library construction. Total RNA is extracted by Trizol (HBE) or RNeasy (HTE)
DNase-seq libraries were prepared as described in Bischof et al. Thorax. 67:385-391. 2012. RNA seq libraries prepared by standard Illumina protocols.


title: HTE RNA-seq rep1

description: processed data: gene_exp.diff


title: LON0116_H1N1_T3

description:
7509336004_A
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)

value: Male


title: CFHBE DNase-seq rep1

description: processed data: DNase_seq_peaks.bed


title: NHBE DNase-seq rep2

description: processed data: DNase_seq_peaks.bed

source_name: NHBE DNase-seq


title: LON0185_H1N1_T1

description:
7509336003_K
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)

value: White


title: HC0012_HC_HC

description:
7509336003_G
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: 2066_HC_HC

description:
7509336004_E
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)


title: LON0149_B_T2

description:
7509336003_I
RNA was isolated using 1.5 ml whole blood and the MagMAX-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion)

value: Yes


title: HBE DNase-seq rep3

description: processed data: DNase_seq_peaks.bed

source_name: HBE DNase-seq


title: HTE DNase-seq rep 2

description: processed data: DNase_seq_peaks.bed

source_name: HTE DNase-seq


title: HBE DNase-seq rep1

description: processed data: DNase_seq_peaks.bed


title: HTE DNase-seq rep 1

description: processed data: DNase_seq_peaks.bed


title: HTE RNA-seq rep4

description: processed data: gene_exp.diff


title: SmallIntestine.CD45-

status: Public on Feb 14 2018

city: hangzhou

first: guoji

last: guo

state: zhejiang

address: yuhangtang road 388

department: Center for Stem Cell and Regenerative Medicine

institute: Zhejiang University School of Medicine

data_processing:
Base-calls were performed with Illumina bcl2fastq
Sequenced reads were trimmed for adaptor sequence.Then reads in bam files were tagged with the cell and molecular (UMI) barcode sequences using Drop-seq_tools-1.12.The cell barcodes are tagged as XC in the bam file and the UMI is tagged as XM in the bam file. The reads quality under 10 were removed.
ScRNA-seq reads were aligned to the Mus_musculus.GRCm38.88 genome assembly using STAR version (2.5.2a)  with default configurations.
Next, merge the STAR alignment tagged bam SAM to recover cell /molecular barcodes and the reads are annotated with exon tags.
Last, we demultiplexed all cell barcodes taken into consideration for the analysis from the exon-tagged bam files to get a digital expression matrix based on UMI counts.
Genome_build: Mus_musculus.GRCm38.88 genome
Supplementary_files_format_and_content: tab-delimited text files of digital expression matrix (dge) based on raw UMI counts ,columns are cells, and rows are genes.

extract_protocol:
Tissue dissociation and  single-cell lysate
Library preparations were performed with the Microwell-seq protocol

value: 6-10 week-old

tag: gender

value: female


title: HTE RNA-seq rep2

description: processed data: gene_exp.diff


title: MammaryGland.Virgin.CD45-.2


title: CFHBE DNase-seq rep3

description: processed data: DNase_seq_peaks.bed


title: MammaryGland.Involution.CD45-.2


title: FetalLiver

source_name: FetalLiver

value: FetalLiver

value: E14.5 embryos


title: EmbryonicMesenchyme.E14.5

value: EmbryonicMesenchyme


title: NHBE DNase-seq rep1

description: processed data: DNase_seq_peaks.bed


title: HBE DNase-seq rep2

description: processed data: DNase_seq_peaks.bed


title: MammaryGland.Involution.CD45-.1


title: MammaryGland.Virgin.CD45-.1


title: endo CT+, chronic endometritis, clinical manifestations of PID - #6

status: Public on Feb 06 2018

city: Chapel Hill

first: xiaojing

last: zheng

address: 111 Mason Farm Road

institute: UNC-CH

description: Sample 64

hyb_protocol: Standard Illumina hybridization protocol

scan_protocol: Standard Illumina scanning protocol

data_processing: The data were normalised using quantile normalisation in R

source_name: human blood

label_protocol: Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays

extract_protocol: RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Alpha and beta globin mRNA was removed from total RNA using Globinclear (Ambion/ThermoFisher Scientific). Quality control was performed with Agilent Bioanalyser.

value: Whole blood

tag: disease state

value: chronic endometritis + chlamydia


title: endo CT+, acute endometritis, clinical manifestations of PID + #4 and #4 biological replicate

description:
Sample 66.1
Sample 66

value: acute endometritis + chlamydia


title: endo CT+, acute endometritis, clinical manifestations of PID + #5 replicate and #5 biological replicate

description:
Sample 67.1
Sample 67


title: STI -, normal histology, clinical manifestations of PID - #9

description: Sample 55

value: normal histology


title: endo CT+, chronic endometritis, clinical manifestations of PID - #2

description: Sample 60


title: endo CT+, chronic endometritis, clinical manifestations of PID - #3

description: Sample 61


title: STI -, normal histology, clinical manifestations of PID - #11

description: Sample 57


title: endo GC+, chronic endometritis, clinical manifestations of PID + #4 replicate and #4 biological replicate

description:
Sample 68.1
Sample 68

value: chronic endometritis + gonorrhea


title: endo CT+, chronic endometritis, clinical manifestations of PID - #5

description: Sample 63


title: STI -, normal histology, clinical manifestations of PID - #12

description: Sample 58


title: endo CT+, chronic endometritis, clinical manifestations of PID - #4

description: Sample 62


title: STI -, normal histology, clinical manifestations of PID - #10

description: Sample 56


title: STI -, normal histology, clinical manifestations of PID - #8

description: Sample 54


title: endo CT+, acute endometritis, clinical manifestations of PID + #3 and #3 biological replicate

description:
Sample 65.1
Sample 65


title: STI -, normal histology, clinical manifestations of PID - #4

description: Sample 50


title: STI -, normal histology, clinical manifestations of PID - #7

description: Sample 53


title: STI -, normal histology, clinical manifestations of PID - #6

description: Sample 52


title: STI -, normal histology, clinical manifestations of PID - #3

description: Sample 49


title: STI -, normal histology, clinical manifestations of PID - #2

description: Sample 48


title: STI -, normal histology, clinical manifestations of PID + #8

description: Sample 46


title: STI -, normal histology, clinical manifestations of PID + #7

description: Sample 45


title: endo CT+, chronic endometritis, clinical manifestations of PID - #1

description: Sample 59


title: STI -, normal histology, clinical manifestations of PID + #6

description: Sample 44


title: STI -, normal histology, clinical manifestations of PID - #1

description: Sample 47


title: STI -, normal histology, clinical manifestations of PID + #4

description: Sample 42


title: STI -, normal histology, clinical manifestations of PID + #3

description: Sample 41


title: STI -, normal histology, clinical manifestations of PID - #5

description: Sample 51


title: STI -, normal histology, clinical manifestations of PID + #5

description: Sample 43


title: STI -, normal histology, clinical manifestations of PID + #2

description: Sample 40


title: STI -, normal histology, clinical manifestations of PID + #1

description: Sample 39


title: STI -, chronic endometritis, clinical manifestations of PID + #6

description: Sample 37

value: chronic endometritis


title: endo CT+, acute endometritis, clinical manifestations of PID + #2

description: Sample 31


title: cervix only STI +, normal histology, clinical manifestations of PID - #15

description: Sample 28


title: STI -, chronic endometritis, clinical manifestations of PID + #2

description: Sample 33


title: STI -, chronic endometritis, clinical manifestations of PID + #7

description: Sample 38


title: endo CT+, acute endometritis, clinical manifestations of PID + #1

description: Sample 30


title: STI -, chronic endometritis, clinical manifestations of PID + #3

description: Sample 34


title: cervix only STI +, normal histology, clinical manifestations of PID - #9

description: Sample 22


title: cervix only STI +, normal histology, clinical manifestations of PID - #1

description: Sample 14


title: STI -, chronic endometritis, clinical manifestations of PID + #4

description: Sample 35


title: cervix only STI +, normal histology, clinical manifestations of PID - #12

description: Sample 25


title: endo GC+, chronic endometritis, clinical manifestations of PID + #3

description: Sample 13


title: cervix only STI +, normal histology, clinical manifestations of PID - #5

description: Sample 18


title: cervix only STI +, normal histology, clinical manifestations of PID - #3

description: Sample 16


title: cervix only STI +, normal histology, clinical manifestations of PID - #7

description: Sample 20


title: cervix only STI +, normal histology, clinical manifestations of PID - #16

description: Sample 29


title: cervix only STI +, normal histology, clinical manifestations of PID - #13

description: Sample 26


title: endo GC+, chronic endometritis, clinical manifestations of PID + #2

description: Sample 12


title: STI -, chronic endometritis, clinical manifestations of PID + #1

description: Sample 32


title: CFHBE RNA-seq rep1

description: processed data: gene_exp.diff

value: donors with Cystic Fibrosis

value: primary human CF bronchial epithelial cells


title: cervix only STI +, normal histology, clinical manifestations of PID - #2

description: Sample 15


title: cervix only STI +, normal histology, clinical manifestations of PID - #10

description: Sample 23


title: cervix only STI +, normal histology, clinical manifestations of PID - #14

description: Sample 27


title: cervix only STI +, normal histology, clinical manifestations of PID - #8

description: Sample 21


title: endo CT and GC +, chronic endometritis, clinical manifestations of PID + #5

description: Sample 10

value: chronic endometritis + chlamydia + gonorrhea


title: STI -, chronic endometritis, clinical manifestations of PID + #5

description: Sample 36


title: cervix only STI +, normal histology, clinical manifestations of PID - #11

description: Sample 24


title: cervix only STI +, normal histology, clinical manifestations of PID - #6

description: Sample 19


title: cervix only STI +, normal histology, clinical manifestations of PID - #4

description: Sample 17


title: THAP_PROseq_rep1

status: Public on Jan 01 2018

city: Ithaca

first: Yumi

last: Woo

state: NY

address: 526 Campus Road

department: Molecular Biology and Genetics

institute: Cornell University

description: nuclear run-on RNA

data_processing:
Whole genome alignment: We aligned all TED-seq reads to the hg38 reference genome as follows. We tagged the sequence reads with the first 8 bases of the reads as the unique molecular identifiers (UMIs). Then we aligned the reads to the hg38 reference genome using the STAR aligner with default parameters. We generated the read count positions (bedgraphs) of the 5′ ends of the mapped reads and collapsed reads that have the same UMI at the same 5′ end positions. These 5′ ends are the positions where the mRNA tail ends are displaced upstream by the selected insert size. We generated pilot TED-seq libraries with the insert sizes of 100 and 300 bp.  3′ transcriptome alignment: When a TED-seq read is split between two exons, we observed the clipping of 5′ end of sequence reads. This lead to inaccurate quantification of the tail length distributions, and we used a complementary mapping approach using the annotated transcriptome.  We used the refFlat mRNA annotation (n=58,135), and extracted the last 400 bp of the transcripts longer than 400 bp (n=55,362; Table 1a). Then we generated a reduced 3′ transcriptome as the reference and aligned TED-seq reads to the new reference using the STAR aligner. We collapsed the UMI and generated 5′ end read distributions for the 300 bp TED-seq libraries for the downstream analysis.
For the RNA-seq data, we used the reads in the last 500 bases of the annotation and generated readcounts, RPKM. For comparison, we generated read counts and RPKMs from the full-length mRNA . We used the cut-off of 10 raw read counts in the RNA-seq dataset. This removes the bimodal distribution of the RPKM readcounts generated by low readcount transcripts.  For the ribosome footprinting normalization, we normalized the reads to total sequenced reads instead of total uniquely mapped reads to account for the global repression in translation. Due to lower mapped read counts in replicates 1 and 2, we used the cut-off of 10 ribosome footprint read counts in DMSO condition as the cut-off in replicates 1 and 2, and 20 read counts in replicates 3 and 4.
PRO-seq raw reads are trimmed by cutadapt using the adaptor sequence of TGGAATTCTCGGGTGCCAAGG and the first 8 bases are tagged as the unique molecular identifiers (UMIs). We aligned the reads to the hg38 genome using STAR limiting the multiple mapping to 1 (uniquely aligned reads only). Reads that share the same mapped positions with the same UMI were collapsed to a single read.  For the promoter read counts, we defined the promoter proximal regions as -50 bp to +250 bp from the hg38 refFlat annotated TSSs (n=61,049), and added all the PRO-seq read counts. Similarly, we defined the post polyadenylation start site (PSS) as from the 3′ end of the annotation to 2 kb downstream, and added all the read counts. These read counts were read per kb per million (RPKM) normalized, using 0.3 kb and 2 kb for the lengths of the promoter proximal region and post-PSS regions.
Genome_build: hg38
Supplementary_files_format_and_content: Processed data files are BedGraph which represents the read count positions of the 5' ends of the mapped reads and collapsed reads that have the same UMI at the same 5' end positions.

source_name: HEK293 cells

growth_protocol: HEK293 cells were cultured in MEM (Gibco) media with 10% fetal bovine serum in a humidified 5% CO2 atmosphere at 37 degrees Celsius.

extract_protocol:
Cell fractionation was performed by differential centrifugation following by THAP treatment (2 μM for 2 hr). Briefly, the cells were washed with cold PBS twice and lysed by cold Buffer L (50 mM Tris-HCL (pH7.6), 50 mM NaCl, 5 mM MgCl2, 0.1% NP-40, 1 mM β-mercaptoethanol, 1x Roche complete protease inhibitor EDTA-free, 10 μl SUPERaseIN/ml) by scraping and pipetting. Cell lysates were homogenized and centrifuged at 2,000g at 4°C for 2 min. Pellet is nucleus fraction and aqua phase includes cytoplasmic fraction. Cytoplasmic fraction was centrifuged at 10,000g at 4°C for 10 min and insoluble fraction was obtained from the pellet. The insoluble fraction includes stress granules in TAHP-treated cells.  For TED-seq, total RNA was isolated using TRIzol or TRIzol-LS according to the manufacturer’s protocol (Invitrogen Life Technologies). Poly(A) RNA was isolated from 5~10 μg of total RNA using Dynabeads® mRNA purification kit (Ambion) according to the manufacturer’s protocol.   The ribosome footprint strategy was followed as the previous paper (Ingolia et al., 2012) and partially modified for the generation of RNA-seq library. In brief, cultured cells were washed twice with ice-cold 1x PBS and lyzed by adding lysis buffer (20 mM Tris-Cl (pH7.4), 150 mM NaCl, 5 mM MgCl2, 1 mM DTT, 100 μg/ml cycloheximide, 1% (v/v) Triton X-100, 25 U/ml Turbo DNase I) and triturating the cells ten times through a 26-gauge needle. After centrifuging the cell lysate at 20,000g at 4°C for 10 min, the supernatant was divided into two groups for ribosome profiling library and mRNA expression profiling library.  For ribosome profiling library, RNase I (100 U/μl) was treated in lysate and incubated at room temperature for 45 min. After nuclease digestion, ribosome footprinted RNAs were purified by sedimenting through a 1 M sucrose cushion and excising the urea gel between 26-34 nucleotides.  For mRNA expression profiling library, TRIzol was added into the supernatant and total RNA was extracted according to the manufacturer's protocol.  For nuclei isolation of PRO-seq, cells were washed twice with 1X cold PBS. Cells were scrapped with 1ml of Buffer D (50mM Tris-Cl, pH 8.0, 25% glycerol, 5mM M MgCl2, 0.1mM EDTA, 5mM DTT). Cells were pelleted at 1000 x g for 5min and then resuspended in 50 µl of Buffer D. And then flash frozen in liquid nitrogen.
In TED-seq libralies, for 3′ RNA adaptor ligation, 40 pmol of RA3 adaptor (5′phosphate-UGGAAUUCUGGGGUGCCAAGG-/3InvdT/-3′) was treated into each poly(A) RNA and incubated in 20°C for 6 hr. After RNA extraction with TRIzol, RNA fragmentation with 1N NaOH was performed by incubating on ice for 10 min. To remove un-ligated adaptors, poly(A) RNA was isolated from the fragmented RNA using Dynabeads® mRNA purification kit (Ambion). 5′ end phosphorylation was performed using T4 polynucleotide kinase (PNK, NEB) according to the manufacturer’s protocol, following by RNA extraction with TRIzol, For 5′ RNA adaptor ligation, 30 pmol of RA5 (5′-GUUCAGAGUUCUACAGUCCGACGAUCNNNNNNNN-3′) and 50 pmol of RT primer (RTP, 5′-GCCTTGGCACCCGAGAATTCCA-3′) were treated into each RNA and incubated in 20°C for 6 hr. 5′-ligated RNA was reverse-transcribed using Superscript II Reverse Transcriptase kit (Invitrogen) including 0.1 M DTT, 12.5 mM dNTP mixture, SUPERaseIN (Ambion). For first size selection of the library, first round amplification was performed with 8 cycles. Following by loading the PCR products to big native 6% polyacrylamide gel, gel bands between 350-360 bp (insert 300 bp) from each sample were excised and then DNA was recovered using Ampure XP beads (Beckman Coulter, Inc.) as provided by the manufacturer’s protocol. To obtain precisely size-selected library, recovered DNA was used for second amplification with optimal cycles and different RPI-X barcode index primer according to TruSeq Library Prep Pooling guide (Illumina). Finally, gel extraction was repeated with bands with 426 bp for each selected insert size. The libraries were pooled and sequenced on Illumina NextSeq500 (75 bp) with PhiX control library and the spike-in mixture.     Ribo-seq libraries, 3′ end was dephosphorylated by adding PNK and ligated by adding 40 pmol of RA3 adaptor. 5′ end was phosphorylated by adding PNK and ATP and ligated by adding 30 pmol of RA5 adaptor and 50 pmol of RTP primer. After reverse transcription using Superscript II reverse transcriptase (Invitrogen), rRNA products were depleted by hybridization to biotinylated sense-strand oligonucleotides followed by removal of the duplexes through streptavidin bead affinity. Finally, through PCR and gel extraction, the library was generated with 150-160 bp-size.    For mRNA-seq library, poly(A) RNA was isolated using Dynabeads® mRNA purification kit (Ambion) according to the manufacturer’s protocol. After RNA fragmentation with 1 N NaOH at 37°C for 3 min, the final library ranging from 150 to 200 bp-size was generated followed by the same process as producing ribosome profiling library from the step of 3′ dephosphorylation, except for rRNA depletion.    PRO-seq libraries were prepared as described previously (Kwak et al., 2013).

treatment_protocol: For ER stress induction, thapsigargin (THAP, Sigma-Aldrich) was treated with 2 µM for 2 h in HEK293 cells.

value: Fetus derived cell line from female human embryonic kidney

tag: passages

tag: drug treatment

value: THAP (2µM) for 2h

tag: sirna transfection


title: DMSO_PROseq_rep2

description: nuclear run-on RNA

value: DMSO for 2h


title: DMSO_PROseq_rep1

description: nuclear run-on RNA


title: THAP_PROseq_rep2

description: nuclear run-on RNA


title: THAP_Riboseq_rep4

description: ribosome-footprinted RNA


title: endo GC+, chronic endometritis, clinical manifestations of PID + #1

description: Sample 11

tag: tissue


title: THAP_Riboseq_rep2

description: ribosome-footprinted RNA


title: endo CT and GC +, chronic endometritis, clinical manifestations of PID + #4

description: Sample 9


title: DMSO_Riboseq_rep4

description: ribosome-footprinted RNA


title: DMSO_Riboseq_rep3

description: ribosome-footprinted RNA


title: THAP_RNAseq_rep3


title: THAP_Riboseq_rep1

description: ribosome-footprinted RNA


title: THAP_RNAseq_rep4


title: DMSO_Riboseq_rep2

description: ribosome-footprinted RNA


title: THAP_Riboseq_rep3

description: ribosome-footprinted RNA


title: DMSO_Riboseq_rep1

description: ribosome-footprinted RNA


title: THAP_RNAseq_rep2


title: DMSO_RNAseq_rep4


title: endo CT+, chronic endometritis, clinical manifestations of PID + #4

description: Sample 4


title: endo CT and GC +, chronic endometritis, clinical manifestations of PID + #2

description: Sample 7


title: endo CT and GC +, chronic endometritis, clinical manifestations of PID + #1

description: Sample 6


title: DMSO_RNAseq_rep3


title: endo CT+, chronic endometritis, clinical manifestations of PID + #3

description: Sample 3


title: endo CT+, chronic endometritis, clinical manifestations of PID + #2

description: Sample 2


title: endo CT+, chronic endometritis, clinical manifestations of PID + #5

description: Sample 5


title: endo CT and GC +, chronic endometritis, clinical manifestations of PID + #3

description: Sample 8


title: DMSO_RNAseq_rep2


title: endo CT+, chronic endometritis, clinical manifestations of PID + #1

description: Sample 1


title: THAP_RNAseq_rep1


title: DMSO_RNAseq_rep1


title: THAP_insoluble_TEDseq_rep2

description: total RNA from cytosol fraction


title: DMSO_insoluble_TEDseq_rep1

description: total RNA from cytosol fraction


title: DMSO_insoluble_TEDseq_rep2

description: total RNA from cytosol fraction


title: THAP_insoluble_TEDseq_rep1

description: total RNA from cytosol fraction


title: THAP_cytosol_TEDseq_rep2

description: total RNA from cytosol fraction


title: THAP_cytosol_TEDseq_rep1

description: total RNA from cytosol fraction


title: DMSO_cytosol_TEDseq_rep2

description: total RNA from cytosol fraction


title: DMSO_cytosol_TEDseq_rep1

description: total RNA from cytosol fraction


title: Sample 3309 - Testis - low boar taint

status: Public on Sep 25 2017

city: Kongens Lyngby

first: Haja

middle: N

last: Kadarmideen

address: Richard Petersens Plads, Building 324

department: Department of Applied Mathematics and Computer Science

country: Denmark

institute: Technical University of Denmark

description: Total RNA-seq

data_processing:
Illumina Casava1.7 software used for basecalling.
Trimmomatic v.0.33 - trimming for bad reads by standard parameters: PE -phred33 \ ILLUMINACLIP:TruSeq3-PE-2.fa:2:30:10 LEADING:3 TRAILING:3 SLIDINGWINDOW:4:15 MINLEN:36
Cutadapt v.1.8.1 - trimming for adapter sequences: -a TACACTCTTTCCCTACACGACGCTCTTCCGATCT -A AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -a GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT -A AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC \ -e 0.1 -O 5 -m 15 --minimum-length 1 STAR aligner v. 020201 - aligned by parameters: --runThreadN 10 --outFilterMultimapNmax 3 --outFilterMismatchNmax 8 --readFilesCommand zcat --outSAMunmapped Within --outReadsUnmapped Fastx >> output.log
samtools v. 1.3.1 by parameters: view -h -F 1792
HT-seq count v. 0.6.0 - genes counted by parameters: -m intersection-nonempty -s reverse -r pos -f sam -a 10 -t exon
Genome_build: Sus scrofa 10.2.79
Supplementary_files_format_and_content: HT-seq count file (.txt) - raw gene counts

source_name: Testis

extract_protocol:
Following slaughter at 100 kg, the liver was extracted from the carcass and 150 mg of tissue were retrieved by punch biopsy and immediately immersed into 1.5 ml RNAlater (QIAGEN, Hilden, Germany) in 2 ml Eppendorf tubes (Eppendorf, Hamburg, Germany). The carcasses were kept in a cold room at 4°C for approximately 1.5 h before 150 mg testis tissue were retrieved by punch biopsy and immersed into 1.5 ml RNAlater (QIAGEN).
Either TruSeq stranded mRNA (Illumina, San Diego, USA) kit following instructions of the manufacturer or TruSeq total stranded RNA (Illumina) kit following instructions of the manufacturer.

value: Testis

tag: group

value: low boar taint


title: Sample 3114 - Testis - low boar taint

description: Total RNA-seq


title: Sample 9137 - Testis - low boar taint

description: stranded mRNA


title: Sample 1101 - Testis - low boar taint

description: Total RNA-seq


title: Sample 7110 - Testis - medium boar taint

description: Total RNA-seq

value: medium boar taint


title: Sample 8311 - Testis - low boar taint

description: stranded mRNA


title: Sample 1325 - Testis - low boar taint

description: Total RNA-seq


title: Sample 7312 - Testis - low boar taint

description: stranded mRNA


title: Sample 3109 - Testis - low boar taint

description: Total RNA-seq


title: Sample 1137 - Testis - low boar taint

description: stranded mRNA


title: Sample 8101 - Testis - low boar taint

description: stranded mRNA


title: Sample 3102 - Testis - low boar taint

description: Total RNA-seq


title: Sample 1113 - Testis - medium boar taint

description: Total RNA-seq


title: Sample 1112 - Testis - low boar taint

description: stranded mRNA


title: Sample 1105 - Testis - low boar taint

description: Total RNA-seq


title: Sample 1314 - Testis - low boar taint

description: Total RNA-seq


title: Sample 1127 - Testis - low boar taint

description: Total RNA-seq


title: Sample 2111 - Testis - medium boar taint

description: Total RNA-seq


title: Sample 9328 - Testis - medium boar taint

description: stranded mRNA


title: Sample 8106 - Testis - medium boar taint

description: stranded mRNA


title: Sample 9135 - Testis - medium boar taint

description: stranded mRNA


title: Sample 8308 - Testis - medium boar taint

description: stranded mRNA


title: Sample 9312 - Testis - low boar taint

description: stranded mRNA


title: Sample 7112 - Testis - medium boar taint

description: stranded mRNA


title: Sample 9304 - Testis - medium boar taint

description: stranded mRNA


title: Sample 8117 - Testis - medium boar taint

description: stranded mRNA