title: Rosi_10mgkg_F_28d_4 [mRNA]


title: Rosi_10mgkg_F_28d_3 [mRNA]

status: Public on May 05 2018

type: RNA

city: Basel

first: Jonathan

last: Moggs

address: Fabrikstrasse 2

country: Switzerland

institute: Novartis

hyb_protocol: 0.6 ug of Cy3-labelled cRNA (specific activity >10.0 pmol Cy3/ug cRNA) was fragmented at 60°C for 30 minutes in a reaction volume of 25ul containing 25x Agilent fragmentation buffer and 10x Agilent blocking agent following the manufacturers instructions. On completion of the fragmentation reaction, 25ul of 2x Agilent HI-RPM Hybridization Buffer was added to the fragmentation mixture and hybridized to Agilent Whole Rat Genome Oligo Microarrays (G4853A) for 17 hours at 65°C in a rotating Agilent hybridization oven.  After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent),  1 minute with 37°C GE Wash buffer 2 (Agilent),  10 Seconds with Acetonitrile (VWR HPLC Grade) and 30 Seconds with Wash 3 Stabilisation and Drying Solution (Agilent).

scan_protocol: Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505C) using one color scan setting for 8x15k array slides (Scan Area 61x21.6 mm, Scan resolution 5um, Dye channel is set to Green and Green PMT is set to 100%).

data_processing: Raw data has been background corrected and quantile normalized using the limma package for R/Bioconductor.

molecule: total RNA

organism: Rattus norvegicus

source_name: bladder, female, Rosiglitazone, 10 mg/kg/day, 28 days

label_protocol: Cyanine-3 (Cy3) labeled cRNA was prepared from 0.2 ug RNA using the One-Color Low Input Quick Amp Labelling  kit (Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA).  Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.

growth_protocol: Sprague-Dawley rats (10/sex/group) were treated orally with vehicle (0.5% methylcellulose 15), Pioglitazone hydrochloride (CAS No 112529-15-4) at 16 or 63 mg/kg/day, or Rosiglitazone maleate (CAS 155141-29-0) at 10 mg/kg/day.

extract_protocol: RNA was isolated using the Qiagen RNeasy kit following the manufacturer's recommendations. For extraction, the filter paper was trimmed, and transferred to the lysis buffer. All handling was done on dry ice.

treatment_protocol: The rats received a daily oral administration of 10 mL/kg body weight of vehicle or test compound solution for either 3, 28 or 90 days. One day after the last treatment, the animals were anaesthetised in isoflurane, exsanguinated from the abdominal aorta and subjected to necropsy. The bladder was rapidly isolated and cut longitudially into halves. The urothelium was scraped off with a scalpel and snap frozen in liquid nitrogen.

tag: tissue

value: bladder urothelium

tag: strain

value: Sprague-Dawley

tag: Sex

value: female

value: Rosiglitazone

value: 10 mg/kg/day

value: 28 days

tag: group

value: yes


title: TFAM OE Aop RNAi rep1

city: London

first: Joseph

middle: M.

last: Bateman

state: (Click to Select US State)

address: Guy's campus

country: United Kingdom

institute: King's College London

description: Gene expression data from CNS tissue over-expressing TFAM combined with Aop knock-down in neurons

hyb_protocol: 7mg of labelled cDNA was hybridised to Affymetrix Drosophila genome v2 GeneChips

scan_protocol: Genechips were washed, stained (GeneChip® Fluidics Station 450) and scanned (GeneChip Scanner 3000 7G) according to manufacturer’s instructions (Nugen Technologies Inc & Affymetrix)

data_processing: Data was processed using a MAS5.0 algorithm and analysed using the Transcriptome Analysis Console (ThermoFisher) using gene level differential expression analysis. Means were calculated using Tukey's Bi-weight average algorithm and differential expression between groups was calculated using un-paired one way one way analysis of variance (ANOVA). A statistical cutoff of p<0.05 was used and a fold change cutoff of ±1.5 fold were used.

label: biotin

organism: Drosophila melanogaster

source_name: CNS tissue from nsybGal4/UAS-TFAM3M + Aop RNAi larvae

label_protocol: 10ng of RNA per genotype was converted into labelled cDNA with the Nugen Ovation V2 protocol (NuGEN Technologies Inc.)

growth_protocol: Larvae were grown on standard fly food at 25 degrees.

extract_protocol: For the microarray analysis the complete CNS from 20 wandering third instar larvae were dissected in PBS and placed into PBS on ice and then transferred into 100ul of lysis buffer from the Absolutely RNA Microprep kit (Stratagene) and vortexed for 5 seconds. Total RNA was then prepared using this kit. For each genotype RNA samples were prepared in triplicate and stored at -80oC.

treatment_protocol: One control and five experimental genotypes were used: Control (nsybGal4/+), Experimental group 1: TFAM3M (nsybGal4>UAS-TFAM3M), Experimental group 2: Pnt RNAi (nsybGal4>UAS-Pnt RNAi #JF02227), Experimental group 3: Aop RNAi (nsybGal4>UAS-Aop RNAi #3166R1), Experimental group 4: TFAM3M, Pnt RNAi (nsybGal4>UAS-TFAM3M, UAS-Pnt RNAi #JF02227), Experimental group 5: TFAM3M, Aop RNAi (nsybGal4>UAS-TFAM3M, UAS-Aop RNAi #3166R1).

tag: genotype/variation

value: nsybGal4/UAS-TFAM3M, Aop RNAi


title: Rosi_10mgkg_F_3d_5 [mRNA]

source_name: bladder, female, Rosiglitazone, 10 mg/kg/day, 3 days

value: 3 days


title: Rosi_10mgkg_F_3d_2 [mRNA]


title: TFAM OE Pnt RNAi rep2

description: Gene expression data from CNS tissue over-expressing TFAM combined with Pnt knock-down in neurons

source_name: CNS tissue from nsybGal4/UAS-TFAM3M + Pnt RNAi larvae

value: nsybGal4/UAS-TFAM3M, Pnt RNAi


title: Rosi_10mgkg_F_28d_1 [mRNA]


title: Rosi_10mgkg_F_3d_4 [mRNA]


title: TFAM OE Pnt RNAi rep3

value: CNS tissue from nsybGal4/UAS-TFAM3M + Pnt RNAi larvae


title: Aop RNAi rep2

description: Gene expression data from CNS with Aop knock-down in neurons

source_name: CNS tissue from nsybGal4/Aop RNAi larvae

value: nsybGal4/Aop RNAi


title: Pio_63mgkg_M_90d_5 [mRNA]

source_name: bladder, male, Pioglitazone, 63 mg/kg/day, 90 days

value: male

value: Pioglitazone

value: 63 mg/kg/day

value: 90 days


title: Aop RNAi rep3

value: CNS tissue from nsybGal4/Aop RNAi larvae


title: Rosi_10mgkg_F_3d_1 [mRNA]

value: bladder urothelium


title: Pio_63mgkg_M_90d_2 [mRNA]

value: Sprague-Dawley


title: Rosi_10mgkg_F_3d_3 [mRNA]

value: female


title: TFAM OE Pnt RNAi rep1


title: Pio_63mgkg_M_90d_3 [mRNA]


title: Pio_63mgkg_M_90d_4 [mRNA]


title: Pio_63mgkg_M_90d_1 [mRNA]


title: Aop RNAi rep1


title: Pnt RNAi rep2

description: Gene expression data from CNS with Pnt knock-down in neurons

source_name: CNS tissue from nsybGal4/Pnt RNAi larvae

value: nsybGal4/Pnt RNAi


title: Pio_63mgkg_M_28d_4 [mRNA]

source_name: bladder, male, Pioglitazone, 63 mg/kg/day, 28 days


title: Pnt RNAi rep1

value: CNS tissue from nsybGal4/Pnt RNAi larvae


title: Pio_63mgkg_M_28d_5 [mRNA]

value: 28 days


title: Pio_63mgkg_M_3d_3 [mRNA]

source_name: bladder, male, Pioglitazone, 63 mg/kg/day, 3 days

value: yes


title: Pnt RNAi rep3


title: Pio_63mgkg_M_28d_2 [mRNA]


title: Pio_63mgkg_M_28d_1 [mRNA]


title: Pio_63mgkg_M_3d_5 [mRNA]

value: no


title: Pio_63mgkg_M_28d_3 [mRNA]


title: Pio_63mgkg_M_3d_2 [mRNA]

value: 3 days


title: Pio_16mgkg_M_90d_5 [mRNA]

source_name: bladder, male, Pioglitazone, 16 mg/kg/day, 90 days

value: 16 mg/kg/day


title: Pio_63mgkg_M_3d_1 [mRNA]


title: TFAM OE rep3

description: Gene expression data from CNS tissue over-expressing TFAM in neurons

source_name: CNS tissue from nsybGal4/UAS-TFAM3M larvae


title: Pio_16mgkg_M_90d_4 [mRNA]


title: Pio_16mgkg_M_90d_3 [mRNA]


title: Pio_63mgkg_M_3d_4 [mRNA]


title: TFAM OE rep2

value: CNS tissue from nsybGal4/UAS-TFAM3M larvae


title: Pio_16mgkg_M_90d_2 [mRNA]


title: control rep2

description: Gene expression data from control CNS tissue

source_name: CNS tissue from nsybGal4/+ control larvae


title: Pio_16mgkg_M_28d_2 [mRNA]

source_name: bladder, male, Pioglitazone, 16 mg/kg/day, 28 days


title: Pio_16mgkg_M_90d_1 [mRNA]


title: Pio_16mgkg_M_28d_1 [mRNA]


title: control rep3

value: CNS tissue from nsybGal4/+ control larvae


title: control rep1


title: Pio_16mgkg_M_28d_3 [mRNA]

value: male


title: Pio_16mgkg_M_3d_4 [mRNA]

source_name: bladder, male, Pioglitazone, 16 mg/kg/day, 3 days

value: Pioglitazone


title: Pio_16mgkg_M_3d_5 [mRNA]


title: Pio_16mgkg_M_3d_1 [mRNA]


title: Rosi_10mgkg_M_90d_2 [miRNA]

hyb_protocol: Entire Cy3-labelled miRNA  was fragmented at 100°C for 5 minutes in a reaction volume of 45ul containing 10x Agilent blocking agent and 2x Agilent hybridization buffer following the manufacturers instructions. On completion of the fragmentation reactionsamples were hybridized to Agilent Rat miRNA Microarrays (G4473B) for 20 hours at 55°C in a rotating Agilent hybridization oven.  After hybridization, microarrays were washed 5 minute at room temperature with GE Wash Buffer 1 (Agilent) and 1 minute with 37°C GE Wash buffer 2 (Agilent).

scan_protocol: Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G25056) using one color scan setting for 8x15k array slides (Scan Area 61x21.6 mm, Scan resolution 5um, Dye channel is set to Green and Green PMT is set to 100%).

data_processing: Raw data was RMA normalized using the limma package for R/Bioconductor.

source_name: bladder, male, Rosiglitazone, 10 mg/kg/day, 90 days

label_protocol: Cyanine-3 (Cy3) labeled miRNA was prepared from 0.1 ug RNA using the microRNA complete Labelling and Hybridisation Kit (Agilent) according to the manufacturer's instructions, followed by purification with  Micro Bio Gel Filtration Columns (Bio-Rad) concentration using a Speed-Vac, 45°C 30 minutes.

treatment_protocol: The rats received a daily oral administration of 10 mL/kg body weight of vehicle or test compound solution for either 3, 28 or 90 days. One day after the last treatment, the animals were anaesthetised in isoflurane, exsanguinated from the abdominal aorta and subjected to necropsy. The bladder was rapidly isolated and cut longitudinally into halves. The urothelium was scraped off with a scalpel and snap frozen in liquid nitrogen.

value: Rosiglitazone

value: 10 mg/kg/day


title: Rosi_10mgkg_M_90d_4 [miRNA]

value: 90 days


title: Rosi_10mgkg_M_90d_3 [miRNA]

value: bladder urothelium


title: Pio_16mgkg_M_3d_2 [mRNA]

value: Sprague-Dawley


title: TFAM OE rep1


title: Pio_63mgkg_F_90d_3 [mRNA]

source_name: bladder, female, Pioglitazone, 63 mg/kg/day, 90 days

value: female

value: 63 mg/kg/day


title: Pio_16mgkg_M_28d_4 [mRNA]


title: Rosi_10mgkg_M_90d_1 [miRNA]


title: Pio_16mgkg_M_3d_3 [mRNA]


title: Pio_63mgkg_F_90d_5 [mRNA]


title: Pio_63mgkg_F_90d_2 [mRNA]


title: Rosi_10mgkg_M_90d_5 [miRNA]


title: Rosi_10mgkg_M_28d_5 [miRNA]

source_name: bladder, male, Rosiglitazone, 10 mg/kg/day, 28 days


title: Rosi_10mgkg_M_28d_4 [miRNA]


title: Pio_63mgkg_F_90d_1 [mRNA]


title: Pio_63mgkg_F_28d_5 [mRNA]

source_name: bladder, female, Pioglitazone, 63 mg/kg/day, 28 days


title: Pio_63mgkg_F_90d_4 [mRNA]


title: Pio_63mgkg_F_3d_5 [mRNA]

source_name: bladder, female, Pioglitazone, 63 mg/kg/day, 3 days


title: Pio_63mgkg_F_28d_1 [mRNA]


title: Rosi_10mgkg_M_28d_2 [miRNA]


title: Rosi_10mgkg_M_28d_1 [miRNA]


title: Pio_63mgkg_F_28d_2 [mRNA]


title: Lgr6-2

type: SRA

city: Shanghai

first: Luo

last: Guo

address: 83# fenyang road, 9# building, room 613

country: China

institute: Fudan University

library_source: transcriptomic

data_processing:
RNA-Seq reads in the fastq file were trimmed using Trimmomatic. Clean reads were mapped to the mouse reference genome (mm10) using TopHat (Trapnell and Schatz 2009) followed by transcript assembly and differential gene expression analysis using Cufflinks. Genes and transcripts were annotated using the RefGene database (NCBI).
Genome_build: mm10
Supplementary_files_format_and_content: .txt file containing FPKM data, p value and fold change

organism: Mus musculus

source_name: Cochlea

extract_protocol: RNA libraries were prepared for sequencing using standard Illumina protocols

value: Cochlea

tag: cell type

value: Lgr6+ progenitors


title: Lgr6-1


title: Lgr5-2

value: Lgr5+ progenitors


title: Pio_63mgkg_F_3d_3 [mRNA]

value: yes


title: Rosi_10mgkg_M_3d_5 [miRNA]

source_name: bladder, male, Rosiglitazone, 10 mg/kg/day, 3 days

value: 3 days


title: Lgr5-1


title: Pio_63mgkg_F_3d_2 [mRNA]

value: no


title: Pio_63mgkg_F_28d_4 [mRNA]

value: 28 days


title: Pio_63mgkg_F_3d_1 [mRNA]


title: Pio_63mgkg_F_28d_3 [mRNA]


title: Pio_63mgkg_F_3d_4 [mRNA]


title: Rosi_10mgkg_M_3d_4 [miRNA]


title: Pio_16mgkg_F_90d_3 [mRNA]

source_name: bladder, female, Pioglitazone, 16 mg/kg/day, 90 days

value: 16 mg/kg/day


title: Pio_16mgkg_F_90d_4 [mRNA]


title: Pio_16mgkg_F_90d_1 [mRNA]


title: Rosi_10mgkg_M_3d_3 [miRNA]

value: male


title: Rosi_10mgkg_M_3d_2 [miRNA]


title: Pio_16mgkg_F_90d_2 [mRNA]

value: Pioglitazone


title: Pio_16mgkg_F_28d_5 [mRNA]

source_name: bladder, female, Pioglitazone, 16 mg/kg/day, 28 days


title: Rosi_10mgkg_M_3d_1 [miRNA]

value: Rosiglitazone


title: Pio_16mgkg_F_28d_2 [mRNA]


title: Rosi_10mgkg_F_90d_5 [miRNA]

source_name: bladder, female, Rosiglitazone, 10 mg/kg/day, 90 days

value: 10 mg/kg/day


title: Rosi_10mgkg_F_90d_4 [miRNA]

value: 90 days


title: Pio_16mgkg_F_28d_3 [mRNA]

value: bladder urothelium


title: Rosi_10mgkg_F_90d_3 [miRNA]

value: Sprague-Dawley


title: Pio_16mgkg_F_28d_1 [mRNA]

value: female


title: Pio_16mgkg_F_28d_4 [mRNA]


title: Pio_16mgkg_F_3d_5 [mRNA]

source_name: bladder, female, Pioglitazone, 16 mg/kg/day, 3 days


title: Pio_16mgkg_F_3d_2 [mRNA]


title: Pio_16mgkg_F_3d_4 [mRNA]


title: Control_M_90d_5 [mRNA]

source_name: bladder, male, vehicle (MC), 0 mg/kg/day, 90 days

value: vehicle (0.5% methylcellulose)

value: 0 mg/kg/day


title: Pio_16mgkg_F_3d_3 [mRNA]


title: Pio_16mgkg_F_3d_1 [mRNA]


title: Control_M_90d_3 [mRNA]


title: ALOD rep6

city: Boston

first: Bradley

last: Maron

state: MA

address: 77 Avenue Louis Pasteur

department: Medicine

country: USA

institute: Brigham & Women's Hospital

description: replicate 3

data_processing:
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to hg19 whole genome using Tophat2
Counting reads was done by HTSeq
Genome_build: hg19
Supplementary_files_format_and_content: tab-delimited text files include counts for each sample ...

organism: Homo sapiens

source_name: Human pulmonary artery

growth_protocol: Cells were grown to confluence using EBM-2TM medium supplemented with 5% fetal bovine serum.

extract_protocol:
RNA was extracted using RNeasy mini kit (Qiagen)
RNA libraries were prepared for sequencing using standard Illumina protocols

treatment_protocol: Six samples were transfected with NEDD9 siRNA (40nM) using Lipofectamine TM2000 and 24 hours later treated with aldosterone (10-7mol/L) or vehicle control for 24 hours. Other 12 samples were directly treated with aldosterone (10-7mol/L) or vehicle control for 24 hours.

tag: treatment

value: aldosterone

value: pulmonary artery endothelial cells

tag: gender


title: ALOD rep4

description: replicate 1


title: Control_M_90d_1 [mRNA]

value: yes


title: Rosi_10mgkg_F_90d_1 [miRNA]


title: Control_M_3d_5 [mRNA]

source_name: bladder, male, vehicle (MC), 0 mg/kg/day, 3 days

value: 3 days


title: Control_M_90d_2 [mRNA]


title: ALOD rep3

description: replicate 3


title: Rosi_10mgkg_F_90d_2 [miRNA]


title: Pio_16mgkg_F_90d_5 [mRNA]


title: Rosi_10mgkg_F_28d_4 [miRNA]

source_name: bladder, female, Rosiglitazone, 10 mg/kg/day, 28 days


title: ALOD rep2

description: replicate 2


title: ALOD rep5

description: replicate 2


title: Rosi_10mgkg_F_28d_5 [miRNA]


title: Control_M_3d_3 [mRNA]


title: ALOD rep1

description: replicate 1


title: V rep6

description: replicate 3

value: vehicle


title: Rosi_10mgkg_F_28d_1 [miRNA]

value: 28 days


title: Rosi_10mgkg_F_28d_2 [miRNA]


title: Rosi_10mgkg_F_28d_3 [miRNA]


title: Control_M_90d_4 [mRNA]


title: V rep5

description: replicate 2


title: V rep4

description: replicate 1


title: Rosi_10mgkg_F_3d_5 [miRNA]

source_name: bladder, female, Rosiglitazone, 10 mg/kg/day, 3 days


title: Rosi_10mgkg_F_3d_4 [miRNA]


title: Control_M_28d_2 [mRNA]

source_name: bladder, male, vehicle (MC), 0 mg/kg/day, 28 days


title: V rep2

description: replicate 2


title: Control_M_28d_5 [mRNA]


title: Control_M_28d_1 [mRNA]


title: Control_F_90d_3 [mRNA]

source_name: bladder, female, vehicle (MC), 0 mg/kg/day, 90 days


title: V rep1

description: replicate 1


title: V rep3

description: replicate 3


title: Control_M_28d_3 [mRNA]


title: Rosi_10mgkg_F_3d_2 [miRNA]


title: Rosi_10mgkg_F_3d_3 [miRNA]


title: Rosi_10mgkg_F_3d_1 [miRNA]


title: Control_F_90d_5 [mRNA]


title: Pio_63mgkg_M_90d_5 [miRNA]

source_name: bladder, male, Pioglitazone, 63 mg/kg/day, 90 days

value: 63 mg/kg/day


title: Control_F_90d_2 [mRNA]


title: Control_M_28d_4 [mRNA]

value: male


title: Pio_63mgkg_M_90d_4 [miRNA]

value: Pioglitazone


title: Control_M_3d_1 [mRNA]


title: Control_F_3d_4 [mRNA]

source_name: bladder, female, vehicle (MC), 0 mg/kg/day, 3 days


title: Control_F_90d_1 [mRNA]


title: Control_F_28d_5 [mRNA]

source_name: bladder, female, vehicle (MC), 0 mg/kg/day, 28 days


title: Pio_63mgkg_M_90d_3 [miRNA]


title: Control_M_3d_4 [mRNA]


title: Control_M_3d_2 [mRNA]


title: WhiB4 Complemented CHP treated 1

city: New York

first: SAURABH

last: MISHRA

state: New York

address: 1300 York Ave, Box 62, New York

department: Microbiology and Immunology

institute: Weill Cornell Medicine

description: Gene expression of WhiB4 complemented strain early log phase cultures 2h after treatment with 0.25 mM CHP in 7H9-ADS medium

hyb_protocol: The total RNA was processed, and hybridized to the Mtb Whole Genome Gene Expression Profiling microarray- G2509F (AMADID: G2509F_034585, Agilent Technologies PLC).  DNA microarrays were provided by the University of Delhi South Campus MicroArray Centre (UDSCMAC).

scan_protocol: Microarray slides were scanned at the resolution of 5µm using Agilent Microarray scanner (G2565CA) with scan control software as per manufacturer's instructions (G2505-90020). Settings included Agilent HD_GX_1colour (61X21.6mm), TIFF 20 bit, and Photomultiplier tube (PMT) gain 100%. Scanned image displays were analyzed by quantifying the pixel density of each hybridization spot using the Agilent Feature Extraction software (v10.5). Local background signals were subtracted from the data automatically by this software and raw data was obtained.

data_processing: Normalization was done to 50th percentile of each sample and data transformation was done to median of all samples using GeneSpring from Agilent Technologies

organism: Mycobacterium tuberculosis H37Rv

source_name: WhiB4 Complemented, CHP treated

label_protocol: Cyanine-3 (Cy3) labeled cRNA was prepared from 50 ng RNA using the One-Color Low RNA Input Quick Amplification Labelling kit (Agilent Technologies, G4140-90040) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA).  Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.

growth_protocol: Mtb H37Rv and WhiB4 Complemented strains were grown till early log phase of ~0.4.

extract_protocol: Gene expression from Treated cultures were fixed using Guanidine thiocyanate, pelleted and total RNA was isolated using FastRNA PRO blue kit (according to manufacturer's instructions). RNA was purified using Qiagen RNeasy plus kit according to manufacturer's instructions. DNAse treatment was done using Ambion TURBO DNA-free kit according to manufacturer's instructions. RNA quality was measured using Agilent Bioanalyzer.

treatment_protocol: Early log phase culture cells were taken. Mtb H37Rv and WhiB4 Complemented strains were treated with  0.25 mM Cumene hydroperoxide (CHP) for 2 h at 37°C.

value: Mtb-delta-whiB4 strain complemented with whiB4

tag: growth

value: early log phase


title: Control_F_90d_4 [mRNA]

value: 90 days


title: Control_F_3d_5 [mRNA]

value: bladder urothelium


title: Control_F_3d_1 [mRNA]

value: Sprague-Dawley


title: WhiB4 Complemented CHP treated 2


title: Control_F_28d_2 [mRNA]

value: female


title: Wt Mtb CHP treated 3

description: Gene expression of Wt Mtb early log phase cultures 2h after treatment with 0.25 mM CHP in 7H9-ADS medium

source_name: Wt Mtb H37Rv, CHP treated

value: Wild type strain of Mtb H37Rv


title: Control_F_3d_3 [mRNA]

value: vehicle (0.5% methylcellulose)

value: 0 mg/kg/day


title: Control_F_28d_4 [mRNA]


title: Control_F_28d_3 [mRNA]


title: Control_F_28d_1 [mRNA]


title: Wt Mtb CHP treated 1


title: WhiB4 Complemented CHP treated 3


title: Pio_63mgkg_M_90d_1 [miRNA]


title: Pio_63mgkg_M_28d_5 [miRNA]

source_name: bladder, male, Pioglitazone, 63 mg/kg/day, 28 days


title: Pio_63mgkg_M_28d_4 [miRNA]


title: Rosi_10mgkg_M_28d_3 [miRNA]

source_name: bladder, male, Rosiglitazone, 10 mg/kg/day, 28 days

value: Rosiglitazone

value: 10 mg/kg/day


title: 200: red earlobe

city: Guangzhou

first: Wei

last: Luo

state: Guangdong

address: Wushan Road

department: The College of Animal Science

institute: South China Agricultural University

library_source: genomic

data_processing:
Library strategy: SLAF-Seq
Raw data was first processed using in-house Perl scripts, to remove reads containing adapter, reads containing over 10 % of ploy-N and low-quality reads (>50 % of bases whose Phred scores were <5 %), thereby obtaining clean reads.
To enable the dependability of the reads for further analysis, the double-end 100 bp of the clean reads were chosen for subsequent analysis. Further, the filtered clean reads were assigned to map the reference genome Gallus Gallus 5.0 by the method of BWA.
With the use of GATK and SAMtools, the SNPs that were both identified by above tools were defined as the true SNPs, resulting in population SNPs.
After removing the integrity less than 80% and the minor allele frequency (MAF) less than 5% of each population SNP, a total of 230,546 qualified SNPs, basically uniformly distributed on genome, was kept for GWAS.
Association of each qualified SNP with earlobe color was performed by the software package PLINK (v1.09), using the case-control association analysis.
Genome_build: Gallus_gallus 5.0
Supplementary_files_format_and_content: Tables including all the filtered SNPs from each sample obtained by SLAF-Seq, and the association p-values of each filtered SNP.

molecule: genomic DNA

organism: Gallus gallus

source_name: Blood

extract_protocol:
Genomic DNA was isolated from whole blood using the NRBC Blood DNA Kit (Omega Bio-Tek, Norcross, GA, USA) following the manufacturer’s instruction.
In this study, the red jungle fowl genome (Gallus_Gallus 4.0, ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA_000002315.2_Gallus_gallus-4.0) was chosen as the reference genome to perform enzyme digestion prediction with in-house related software. The fragments predictably digested by the enzyme that was selected for genome digestion should be in line with below rules, including a low proportion of that locating repetitive sequences, a uniform distribution across the genome to the greatest extent, a preferable identity between the fragment length and the experiment system and the digested fragments number predicted meeting the expectation (i.e., 100,000 SLAF). We herein selected restriction enzyme HaeIII to digest QYP DNA samples, predictably giving rise to 113,202 SLAF, with the length ranging from 364-444 bp. After digested by HaeIII, the genomic DNA fragments (SLAF) from each sample was added with A-tailing at 3’ end, further ligated with Dual-index adaptor, and then amplified by PCR. After that, SLAF from each sample was purified and then mixed for further target fragment selection via gel electrophoresis and extraction, thereby obtaining the SLAF library for high-sequencing. Ultimately, SLAF libraries from each sample were sequenced on Illumina HiSeq 2500 platform, according to Illumina instructions (operated by Biomarker Biological Technology Co., Ltd., Beijing, China). We herein developed a total of 622,443 SLAF markers, including 424,810 polymorphic ones, and their corresponding average sequencing depth reached 10.03 ×.

tag: breed

value: Qiangyuan Partridge chicken (QYP)

value: Blood

tag: phenotype

value: red earlobe


title: 193: red earlobe


title: 192: red earlobe


title: 199: red earlobe


title: 197: red earlobe


title: 198: red earlobe


title: Pio_63mgkg_M_28d_3 [miRNA]

value: 63 mg/kg/day

value: 28 days

value: yes


title: Pio_63mgkg_M_28d_2 [miRNA]

value: male

value: Pioglitazone


title: Pio_63mgkg_M_90d_2 [miRNA]

value: 90 days


title: 190: red earlobe


title: 188: red earlobe


title: 189: red earlobe


title: 195: red earlobe


title: Pio_63mgkg_M_3d_5 [miRNA]

source_name: bladder, male, Pioglitazone, 63 mg/kg/day, 3 days

value: bladder urothelium

value: 3 days


title: Pio_63mgkg_M_28d_1 [miRNA]

value: Sprague-Dawley


title: 196: red earlobe


title: 187: red earlobe


title: 186: red earlobe


title: Control_F_3d_2 [mRNA]

value: female


title: 184: red earlobe


title: 194: red earlobe


title: 185: red earlobe


title: 191: red earlobe


title: 183: red earlobe


title: Pio_63mgkg_M_3d_3 [miRNA]


title: 182: red earlobe


title: 181: red earlobe


title: Pio_63mgkg_M_3d_2 [miRNA]


title: 178: red earlobe


title: 179: red earlobe


title: 177: red earlobe


title: 180: red earlobe


title: 175: red earlobe


title: Pio_16mgkg_M_90d_5 [miRNA]

source_name: bladder, male, Pioglitazone, 16 mg/kg/day, 90 days

value: 16 mg/kg/day


title: Pio_63mgkg_M_3d_1 [miRNA]


title: 173: red earlobe


title: 176: red earlobe


title: 170: red earlobe


title: 172: red earlobe


title: 174: red earlobe


title: 169: red earlobe


title: 167: red earlobe


title: Pio_16mgkg_M_90d_1 [miRNA]


title: 166: red earlobe


title: Pio_16mgkg_M_90d_4 [miRNA]


title: Pio_16mgkg_M_90d_3 [miRNA]


title: Pio_63mgkg_M_3d_4 [miRNA]


title: Pio_16mgkg_M_90d_2 [miRNA]


title: Pio_16mgkg_M_28d_4 [miRNA]

source_name: bladder, male, Pioglitazone, 16 mg/kg/day, 28 days


title: Wt Mtb CHP treated 2

value: Wild type strain of Mtb H37Rv

value: early log phase


title: 164: red earlobe


title: Pio_16mgkg_M_28d_3 [miRNA]


title: 163: red earlobe


title: Pio_16mgkg_M_28d_2 [miRNA]


title: 162: red earlobe


title: 161: red earlobe


title: 171: red earlobe


title: 168: red earlobe


title: 155: red earlobe


title: 159: red earlobe


title: 156: red earlobe


title: 158: red earlobe


title: Pio_16mgkg_M_28d_1 [miRNA]


title: Pio_16mgkg_M_3d_5 [miRNA]

source_name: bladder, male, Pioglitazone, 16 mg/kg/day, 3 days


title: 157: red earlobe


title: Pio_16mgkg_M_3d_4 [miRNA]


title: Pio_16mgkg_M_3d_2 [miRNA]


title: 165: red earlobe


title: 152: red earlobe


title: 154: red earlobe


title: 149: red earlobe


title: Pio_16mgkg_M_3d_1 [miRNA]


title: 151: red earlobe


title: 153: red earlobe


title: Pio_16mgkg_M_3d_3 [miRNA]


title: 147: red earlobe


title: 160: red earlobe


title: 146: red earlobe


title: 145: red earlobe


title: Pio_63mgkg_F_90d_4 [miRNA]

source_name: bladder, female, Pioglitazone, 63 mg/kg/day, 90 days


title: Pio_63mgkg_F_90d_3 [miRNA]


title: 141: red earlobe


title: 143: red earlobe


title: 139: red earlobe


title: 142: red earlobe


title: 140: red earlobe


title: 138: red earlobe


title: Pio_63mgkg_F_90d_1 [miRNA]


title: Pio_63mgkg_F_90d_2 [miRNA]


title: Pio_63mgkg_F_28d_5 [miRNA]

source_name: bladder, female, Pioglitazone, 63 mg/kg/day, 28 days


title: 150: red earlobe


title: 148: red earlobe


title: 137: red earlobe


title: 136: red earlobe


title: 134: red earlobe


title: 132: red earlobe


title: 135: red earlobe


title: 130: red earlobe


title: Pio_63mgkg_F_90d_5 [miRNA]


title: 131: red earlobe


title: Pio_63mgkg_F_28d_1 [miRNA]


title: 133: red earlobe


title: Pio_63mgkg_F_28d_2 [miRNA]


title: 98: white earlobe

value: white earlobe


title: Pio_63mgkg_F_28d_3 [miRNA]


title: 100: white earlobe

value: Qiangyuan Partridge chicken (QYP)


title: 99: white earlobe

value: Blood


title: 144: red earlobe

value: red earlobe


title: Pio_63mgkg_F_3d_5 [miRNA]

source_name: bladder, female, Pioglitazone, 63 mg/kg/day, 3 days

value: 63 mg/kg/day


title: 129: red earlobe


title: 97: white earlobe


title: 96: white earlobe


title: 95: white earlobe


title: 94: white earlobe


title: 128: red earlobe


title: Pio_63mgkg_F_3d_2 [miRNA]


title: 93: white earlobe


title: Pio_63mgkg_F_3d_3 [miRNA]


title: Pio_63mgkg_F_28d_4 [miRNA]

value: 28 days


title: 92: white earlobe


title: 127: red earlobe


title: 91: white earlobe


title: Pio_63mgkg_F_3d_1 [miRNA]

value: yes


title: Pio_63mgkg_F_3d_4 [miRNA]


title: 89: white earlobe


title: 126: red earlobe


title: 125: red earlobe


title: 88: white earlobe


title: 124: red earlobe


title: 87: white earlobe


title: 85: white earlobe


title: Pio_16mgkg_F_90d_5 [miRNA]

source_name: bladder, female, Pioglitazone, 16 mg/kg/day, 90 days


title: Pio_16mgkg_F_90d_4 [miRNA]


title: 84: white earlobe


title: 123: red earlobe


title: Pio_16mgkg_F_90d_3 [miRNA]


title: Pio_16mgkg_F_90d_2 [miRNA]


title: 83: white earlobe


title: 82: white earlobe


title: 81: white earlobe


title: 122: red earlobe


title: Pio_16mgkg_F_90d_1 [miRNA]


title: 80: white earlobe


title: 121: red earlobe


title: Pio_16mgkg_F_28d_5 [miRNA]

source_name: bladder, female, Pioglitazone, 16 mg/kg/day, 28 days

value: no


title: 120: red earlobe


title: 79: white earlobe


title: 90: white earlobe


title: Pio_16mgkg_F_28d_4 [miRNA]

value: Pioglitazone


title: 86: white earlobe


title: 119: red earlobe


title: Pio_16mgkg_F_28d_3 [miRNA]


title: Pio_16mgkg_F_28d_2 [miRNA]


title: 117: red earlobe


title: 75: white earlobe


title: 118: red earlobe


title: 76: white earlobe


title: 116: red earlobe


title: Pio_16mgkg_F_28d_1 [miRNA]


title: 115: red earlobe


title: Pio_16mgkg_F_3d_5 [miRNA]

source_name: bladder, female, Pioglitazone, 16 mg/kg/day, 3 days


title: 74: white earlobe


title: 73: white earlobe


title: Pio_16mgkg_F_3d_4 [miRNA]


title: 114: red earlobe


title: Pio_16mgkg_F_3d_3 [miRNA]


title: 72: white earlobe


title: 71: white earlobe


title: 78: white earlobe


title: 70: white earlobe


title: 77: white earlobe


title: 111: red earlobe


title: Pio_16mgkg_F_3d_1 [miRNA]


title: 69: white earlobe


title: 112: red earlobe


title: 110: red earlobe


title: Control_M_90d_5 [miRNA]

source_name: bladder, male, vehicle (MC), 0 mg/kg/day, 90 days

value: male

value: vehicle (0.5% methylcellulose)

value: 0 mg/kg/day

value: 90 days


title: Control_M_90d_4 [miRNA]

value: bladder urothelium

value: Sprague-Dawley


title: 109: red earlobe


title: Control_M_90d_3 [miRNA]


title: 108: red earlobe


title: 67: white earlobe


title: 66: white earlobe


title: 65: white earlobe


title: Control_M_90d_2 [miRNA]


title: 64: white earlobe


title: 113: red earlobe


title: 105: red earlobe


title: Control_M_3d_5 [miRNA]

source_name: bladder, male, vehicle (MC), 0 mg/kg/day, 3 days

value: 3 days


title: Control_M_90d_1 [miRNA]


title: 106: red earlobe


title: Pio_16mgkg_F_3d_2 [miRNA]

value: female

value: 16 mg/kg/day


title: Control_M_3d_4 [miRNA]


title: Control_M_3d_3 [miRNA]


title: 63: white earlobe

value: Qiangyuan Partridge chicken (QYP)

value: white earlobe


title: 104: red earlobe

value: Blood

value: red earlobe


title: 62: white earlobe


title: 68: white earlobe


title: 60: white earlobe


title: 103: red earlobe


title: 102: red earlobe


title: 61: white earlobe


title: 101: red earlobe


title: 107: red earlobe


title: 59: white earlobe


title: Control_M_3d_1 [miRNA]


title: Control_M_28d_5 [miRNA]

source_name: bladder, male, vehicle (MC), 0 mg/kg/day, 28 days

value: 28 days


title: Control_M_3d_2 [miRNA]

value: yes


title: 30: white earlobe


title: 29: white earlobe


title: 57: white earlobe


title: Control_M_28d_3 [miRNA]


title: Control_M_28d_4 [miRNA]


title: 56: white earlobe


title: 28: white earlobe


title: 26: white earlobe


title: 27: white earlobe


title: Control_M_28d_2 [miRNA]

value: no


title: Control_M_28d_1 [miRNA]


title: Control_F_90d_5 [miRNA]

source_name: bladder, female, vehicle (MC), 0 mg/kg/day, 90 days


title: 55: white earlobe


title: 54: white earlobe


title: 53: white earlobe


title: 24: white earlobe


title: Control_F_90d_4 [miRNA]


title: Control_F_90d_3 [miRNA]


title: 52: white earlobe


title: 23: white earlobe


title: 51: white earlobe


title: 58: white earlobe


title: 22: white earlobe


title: Control_F_90d_1 [miRNA]


title: Control_F_90d_2 [miRNA]


title: 50: white earlobe


title: 49: white earlobe


title: 21: white earlobe


title: Control_F_3d_4 [miRNA]

source_name: bladder, female, vehicle (MC), 0 mg/kg/day, 3 days


title: Control_F_3d_5 [miRNA]


title: 48: white earlobe


title: 18: white earlobe


title: 25: white earlobe


title: 20: white earlobe


title: 19: white earlobe


title: 47: white earlobe


title: 46: white earlobe


title: Control_F_3d_2 [miRNA]


title: 45: white earlobe


title: 17: white earlobe


title: 16: white earlobe


title: 15: white earlobe


title: 44: white earlobe


title: Control_F_28d_5 [miRNA]

source_name: bladder, female, vehicle (MC), 0 mg/kg/day, 28 days


title: 14: white earlobe

source_name: Blood


title: 43: white earlobe


title: Control_F_28d_3 [miRNA]


title: Control_F_28d_4 [miRNA]


title: 42: white earlobe


title: 13: white earlobe


title: 41: white earlobe


title: 12: white earlobe


title: wt+IAA, replicate 3

city: Davis

first: Johan

last: Leveau

state: CA

address: One Shields Ave

department: Plant Pathology

institute: University of California, Davis

data_processing:
50bp single-end reads were subjected to adapter trimming using Scythe (https://github.com/vsbuffalo/scythe), followed by base-quality trimming by sickle (https://github.com/najoshi/sickle). Bases that have quality less than 30 were trimmed. All reads that are less than 30 were removed. Reads that have passed the above quality control were aligned to SILVA rRNA database v123.1 to remove ribosomal RNA contamination
After ribosomal RNA removal, reads were aligned to LF7a genome using Rockhopper. Parameters were set to account for the second-strand sequencing protocol. Raw-counts data to each annotated feature in the genome were generated by Rockhopper.
Genes that have expression less than 1 counts-per-million in all samples were filtered prior to analysis, resulting in 4582 genes. Counts were normalized using calcNormFactors function from edgeR package. Differential expression analyses were conducted using limma-voom (http://www.genomebiology.com/2014/15/2/R29). Factors used to build a Generalized Linear Model include Strain and Treatment, as well as the interaction effect between these two factors.
Supplementary_files_format_and_content: raw counts

organism: Enterobacter soli

source_name: bacterial cells

growth_protocol: Strains were grown overnight at 28°C, with shaking at 250 rpm, in Lysogeny Broth (LB) supplemented with chloramphenicol (CM) 25 ug/ml (for mutant strain only). After centrifugation of overnight cultures at 2,500 xg for 5 minutes, bacterial pellets were resuspended in sterile phosphate buffered saline (PBS), centrifuged and resuspended again, and diluted 100-fold in M9 minimal medium containing 12.5 mM succinate, 0.2% casamino acids, 6.58 μM FeSO4. M9 cultures were incubated at 28°C, with shaking at 250 rpm.

extract_protocol:
After 2 hours of additional incubation, all 12 cultures (2 strains, with or without IAA, 3 replicates for each) were treated with 3 volumes of Bacterial RNAprotect (Qiagen), followed by total RNA isolation using an RNEasy kit (Qiagen) as per the manufacturer’s instructions, which included the DNAseI treatment. RNA quality and concentration were checked on an Agilent Bioanalyzer prior to submission to the UC Davis Expression Analysis Core Facility for ribosomal RNA depletion, cDNA synthesis, and library preparation.
RNA libraries were prepared using standard protocols for Illumina HiSEQ4000 SR50 platform

treatment_protocol: After 3 hours of growth in M9 medium with succinate, three cultures each of wildtype E. soli LF7 and E. soli LF7 ΔiacR::cat were spiked with 200 μM IAA (Sigma Aldrich Pvt Ltd, Bangalore, India), while three cultures of each strain were spiked with water (control).

tag: strain-genotype

value: LF7 wild-type

value: IAA-inducible iac expression; CM susceptible

value: 2 hours exposure to 200µM IAA during exponential growth on succinate


title: 11: white earlobe


title: wt+IAA, replicate 2


title: Control_F_28d_1 [miRNA]

value: vehicle (0.5% methylcellulose)

value: 0 mg/kg/day


title: Control_F_28d_2 [miRNA]

value: bladder urothelium


title: wt-IAA, replicate 3

value: no exposure to IAA during growth on succinate


title: 8: white earlobe


title: 9: white earlobe


title: 10: white earlobe


title: 37: white earlobe


title: wt+IAA, replicate 1


title: Atm-/- -3

city: Shenzhen

first: Min

middle: Xian

last: Qian

address: 3688 Nanhai Ave

department: Health Science Center

institute: Shenzhen University

data_processing:
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome using FANSe 2 with parameters -L55 -E4 -I0 -S10 -M0 -U0 -B1
Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using a protocol from Chepelev et al., Nucleic Acids Research, 2009. In short, exons from all isoforms of a gene were merged to create one meta-transcript. The number of reads falling in the exons of this meta-transcript were counted and normalized by the size of the meta-transcript and by the size of the library.
Genome_build: mm10
Supplementary_files_format_and_content: text files include RPKM values for each Sample

source_name: murine embryonic fibroblast cell

growth_protocol: MEF cells were cultured in DMEM (Gibco®) with 10% FBS.

extract_protocol:
Total RNA extracts are first treated with DNase I to degrade DNA contamination. Next, by using the oligo (dT) magnetic beads the mRNA is enriched . Mixed with the fragmentation buffer, the mRNA is fragmented into short fragments. The first strand of cDNA is synthesized by using random hexamer-primer. Buffer, dNTPs, RNase H and DNA polymerase I are added to synthesize the second strand. The double strand cDNA is purified with AMPure XP beads. End reparation and 3’-end single nucleotide A (adenine) addition is then performed. Finally, sequencing adaptors are ligated to the fragments. The fragments are enriched by PCR amplification.
RNA libraries were prepared for sequencing using standard Illumina protocols.

treatment_protocol: MEF cells were generated from wildtype, Sirt6-/-, P53-/-, or Atm-/-P53-/- mice.

value: murine embryonic fibroblast cell

tag: genotype

value: Atm-/-


title: 35: white earlobe


title: Atm-/- -2


title: 6: white earlobe


title: 7: white earlobe


title: mut+IAA, replicate 3

value: LF7 delta-iacR::cat

value: constitutive iac expression; CMr


title: Sirt6-/- -3


title: 34: white earlobe

value: Qiangyuan Partridge chicken (QYP)

value: white earlobe


title: Atm-/- -1


title: wt-IAA, replicate 1


title: 5: white earlobe

value: Blood


title: 39: white earlobe


title: Control_F_3d_3 [miRNA]

value: Sprague-Dawley

value: female

value: 3 days


title: 38: white earlobe


title: 33: white earlobe


title: Sirt6-/- -2


title: wt-IAA, replicate 2

value: LF7 wild-type


title: 4: white earlobe


title: mut+IAA, replicate 2


title: mut-IAA, replicate 3


title: 36: white earlobe


title: 40: white earlobe


title: Sirt6-/- -1


title: 3: white earlobe


title: WT -3

value: WT


title: 2: white earlobe


title: 31: white earlobe


title: mut-IAA, replicate 2


title: WT -1


title: WT -2


title: Control_F_3d_1 [miRNA]

value: yes


title: mut-IAA, replicate 1


title: mut+IAA, replicate 1

value: 2 hours exposure to 200µM IAA during exponential growth on succinate


title: 32: white earlobe


title: OA_Cart_4_10

status: Public on May 04 2018

city: La Jolla

first: Padma

last: Natarajan

address: 10550 N.Torrey Pines Road

department: CCBB

institute: The Scripps Research Institute

description:
normalized_counts.xlsx
raw_counts.xlsx

data_processing:
Raw data QC of the fastq files was performed with FastQC (v0.10.1). Raw data were aligned to the human genome (hg19) using the STAR aligner v(2.5.3a).
Aligned reads were quantified using HTSeq-count using UCSC RefSeq hg19 annotation (Release 57).
Raw counts were transformed into counts per million and normalized with TMM normalization.
Limma package (version 3.36.0) with its voom method,was used to assess differential expression and perform gene set testing.
Genome_build: hg19
Supplementary_files_format_and_content: Excel file: OabyControl_allgenes.xlsx (results from differential gene expression analyses)

source_name: Knee articular cartilage

extract_protocol:
Cartilage was stored at -20ºC in Allprotect Tissue Reagent  immediately after harvest until RNA extraction. For RNA isolation, a minimum of 150 mg of cartilage (dry weight) was pulverized and homogenized in Qiazol Lysis Reagentat a concentration of 25mg tissue sample per 700µl Qiazol. To remove proteins and cellular debris, a initial phenol-chloroform extraction was performed. Briefly, samples were mixed with 0.2 volumes of chloroform, incubated for 5 minutes in ice, and centrifuged a t 14000 rpm for 15 minutes at 4ºC. The aqueous phase was collected, mixed with 1 volume of Qiazol and incubated for 30 minutes in ice. Then, samples were mixed with 1 volume of 100% ethanol, loaded into a mRNeasy Mini kit column and digested on-column with DNAse following manufacturer instructions. RNA was eluted in 15µl of RNase-free water.
mRNA libraries were prepared using the Encore Complete RNA-Seq DR Multiplex System 1-8 and 9-16 (NuGen) with 16 unique indexed adapters.

tag: age

value: F

tag: oa grade


title: OA_Cart_9_6

description:
normalized_counts.xlsx
raw_counts.xlsx

value: M


title: OA_Cart_1_7

description:
normalized_counts.xlsx
raw_counts.xlsx


title: OA_Cart_3_9

description:
normalized_counts.xlsx
raw_counts.xlsx


title: OA_Cart_8_5

description:
normalized_counts.xlsx
raw_counts.xlsx


title: OA_Cart_2_8

description:
normalized_counts.xlsx
raw_counts.xlsx


title: OA_Cart_10_9

description:
normalized_counts.xlsx
raw_counts.xlsx


title: OA_Cart_7_2

description:
normalized_counts.xlsx
raw_counts.xlsx


title: OA_Cart_5_5

description:
normalized_counts.xlsx
raw_counts.xlsx


title: Normal_Cart_10_8

description:
normalized_counts.xlsx
raw_counts.xlsx


title: Normal_Cart_6_6

description:
normalized_counts.xlsx
raw_counts.xlsx


title: Normal_Cart_5_5

description:
normalized_counts.xlsx
raw_counts.xlsx


title: Normal_Cart_4_4

description:
normalized_counts.xlsx
raw_counts.xlsx


title: OA_Cart_6_1

description:
normalized_counts.xlsx
raw_counts.xlsx


title: Normal_Cart_3_3

description:
normalized_counts.xlsx
raw_counts.xlsx


title: 1: white earlobe


title: Treat

city: guangzhou

first: Li

middle: qian

last: qian

institute: Southern Medical University

description: The Hy3TM-labeled samples were hybridized on the miRCURYTM LNA Array (Exiqon) containing 1891 unique miRNAs. Hy5 was not used in this experiment.

hyb_protocol: After stopping the labeling procedure, the Hy3TM-labeled samples were hybridized on the miRCURYTM LNA Array (Exiqon) according to array manual. The total 25 μL mixture from Hy3TM-labeled samples with 25 μL hybridization buffer were first denatured for 2 min at 95°C, incubated on ice for 2 min and then hybridized to the microarray for 16-20 h at 56°C in a 12-Bay Hybridization Systems (Hybridization System - NimbleGen Systems, Inc., Madison, WI, USA), which provides an active mixing action and constant incubation temperature to improve hybridization uniformity and enhance signal. Following hybridization, the slides were achieved, washed several times using Wash buffer kit (Exiqon), and finally dried by centrifugation for 5 min at 400 rpm.

scan_protocol: The slides were scanned using the Axon GenePix 4000B microarray scanner (Axon Instruments, Foster City, CA). Scanned images were then imported into GenePix Pro 6.0 software (Axon) for grid alignment and data extraction. Replicated miRNAs were averaged and miRNAs that intensities>=30 in all samples were chosen for calculating normalization factor.

data_processing: Expressed data were normalized using the Median normalization. After normalization, differentially expressed miRNAs were identified through Fold Change filtering. Hierarchical clustering was performed using MEV software (v4.6, TIGR).

source_name: H460 cells treated with sulforaphane

label_protocol: After RNA isolation from the samples, the miRCURY™ Hy3™/Hy5™ Power labeling kit (Exiqon, Vedbaek, Denmark) was used according to the manufacturer's guideline for miRNA labelling.

growth_protocol: Human NSCLC cell line H460 was maintained in DMEM supplemented with 10% fetal bovine serum.

extract_protocol: Total RNA was isolated using TRIzol (Invitrogen) and miRNeasy mini kit (QIAGEN) according to manufacturer's instructions, which efficiently recovered all RNA species, including miRNAs.

treatment_protocol: H460 cells were treated with vehicle or SFN for 48 hours.

tag: cell line

value: non-small cell lung cancer (NSCLC) cell line

value: sulforaphane


title: Ctrl

source_name: H460 cells treated with vehicle

value: vehicle


title: Normal_Cart_2_2

description:
normalized_counts.xlsx
raw_counts.xlsx


title: MCECs, normal glucose

city: Shinheung- Dong, Jung-Gu Incheon

first: GUO NAN

last: YIN

address: 7-206, 3rd ST B dong, 3 floor room 307-2

department: Urology

country: South Korea

institute: Inha university hospital

description: Pooling : normal 1 + normal 2 + normal 3 = Normal

hyb_protocol: After checking labeling efficiency, fragmentation of cRNA was performed by adding 10X blocking agent and 25X fragmentation buffer and incubating at 60oC for 30 min. The fragmented cRNA was resuspended with 2X hybridization buffer and directly pipetted onto assembled Agilent’s Canine Oligo Microarray (44K). The arrays hybridized at 65oC for 17 hours using Agilent Hybridization oven (Agilent Technology, USA). The hybridized microarrays were washed as the manufacturer’s washing protocol (Agilent Technology, USA).

scan_protocol: The hybridized images were scanned using Agilent’s DNA microarray scanner and quantified with Feature Extraction Software (Agilent Technology, Palo Alto, CA).

data_processing: All data normalization and selection of fold-changed genes were performed using GeneSpringGX 7.3 (Agilent Technology, USA). The averages of normalized ratios were calculated by dividing the average of normalized signal channel intensity by the average of normalized control channel intensity. Functional annotation of genes was performed according to Gene OntologyTM Consortium (http://www.geneontology.org/index.shtml) by GeneSpringGX 7.3. Gene classification was based on searches done by BioCarta (http://www.biocarta.com/), GenMAPP (http://www.genmapp.org/), DAVID (http://david.abcc.ncifcrf.gov/), and Medline databases (http://www.ncbi.nlm.nih.gov/).

label_protocol: Amplified and labeled cRNA was purified on cRNA Cleanup Module (Agilent Technology) according to the manufacturer’s protocol. Labeled cRNA target was quantified using ND-1000 spectrophotometer (NanoDrop Technologies, Inc., Wilmington, DE).

growth_protocol: The mouse penis cavernous primary endothelial cell was maintained in Medium 199 (M199) containing 20% heat-inactivated FBS,1% penicillin/streptomycin, 0.5 mg/ml heparin (Sigma-Aldrich), and 5 ng/ml vascular endothelial growth factor A (R&D Systems Inc.)

extract_protocol: Total RNA extracted using Trizol following manufacturer's instructions.

treatment_protocol: MCECs cells at the density of 2 × 106 cells/well were exposed to the normal-glucose (5 mmol, Sigma-Aldrich), high-glucose (30 mmol) or high-glucose+ DKK2 (A&R Therapeutics) condition for 48 hours.


title: ZWU19PlasmaB53A17

first: Thomas

last: Tuschl

state: NY

address: 1230 York Avenue

institute: The Rockefeller University

data_processing:
All FASTQ files were processed uniformly through the extra-cellular RNA sequencing data analysis platform (RSDAP) (Farazi T et. al, 2002, Methods 58:171-187), considering a size range of 16 to 45 nt and allowing up to two mismatches to the curated reference-transcriptome.
Genome_build: hg19
Supplementary_files_format_and_content: Each processed data file contains miRNA read counts and miRNA read frequencies for miRNA species found in a given sample.

source_name: Plasma

extract_protocol:
Low speed centrifugation (2500 g x 15 minutes, room temperature) was performed, then transfer of supernatant, 2nd round of centrifugation (same parameters), then transfer of supernatant. A self-developed method and self-developed solutions were used for nucleic-acid extraction, nucleic-acid partitioning, and column purification (RNA isolation). Extracellular vesicles were not pre-purified. RNA isolation kit was a self developed protocol executed on epMotion 5075 liquid handling system. exRNA were quantified via use of two sets of spike-in calibrators added 1) prior to RNA isolation (set1), 2) at prior to cDNA library preparation (set2), to determine RNA quality and yield of miRNAs and other RNA molecules with 5P/3OH ends. Each set consists of ten individual 22-nt calibrators that do not map to the human genome. Relative to the material present in each sample cDNA library preparation, 1 attomole of each calibrator oligo was present unless stated otherwise. Calibrator sequences (labeled by set) are: >Set1_cali_01_rc TCCACGACGTCTCATGTATTTC, >Set1_cali_04_rc GGGTACCATACCGGTTGTCTTA, >Set1_cali_17_rc TCATGAGTCCGTACCTTGATTG, >Set1_cali_18_rc ATCATTTACGATTCGGAGCTGT, >Set1_cali_20_rc GATAGTTCGGGATCGCTGTAAC, >Set1_cali_24_rc TGCTACTCCGATCTTTAGCCTC, >Set1_cali_25_rc AGGGCCCTTTAGGCACTAATAG, >Set1_cali_27_rc GTAGCTGTCAGTACGTTCGTGC, >Set1_cali_43_rc TCTAGTTGCGTGATGGAGAGAA, >Set1_cali_44_rc AGCCGCATTTCGTAGTGATATT, >Set2_cali_07_rc GTCCCACTCCGTAGATCTGTTC, >Set2_cali_11_rc GATGTAACGAGTTGGAATGCAA, >Set2_cali_12_rc TAGCATATCGAGCCTGAGAACA, >Set2_cali_14_rc CATCGGTCGAACTTATGTGAAA, >Set2_cali_15_rc GAAGCACATTCGCACATCATAT, >Set2_cali_16_rc TCTTAACCCGGACCAGAAACTA, >Set2_cali_26_rc AGGTTCCGGATAAGTAAGAGCC, >Set2_cali_28_rc TAACTCCTTAAGCGAATCTCGC, >Set2_cali_31_rc AAAGTAGCATCCGAAATACGGA, >Set2_cali_35_rc TGATACGGATGTTATACGCAGC
Self developed protocol - 24 samples were multiplexed into one processing batch and their cDNA was sequenced using Illumina HiSeq flowcell.

tag: condition

value: Healthy Subject

tag: anatomical location

value: Entire cardiovascular system

tag: rna source

value: total cell-free biofluid RNA

tag: rna isolation kit

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

tag: shortid

tag: individual

tag: time point

value: B9 (Day 29, 1 Hour After Lunch)

tag: nutrition state

value: postE (Postprandial Early, 1 Hour After Lunch)

tag: female hormonal cycle phase

value: L

tag: female hormonal cycle length

tag: replicate

value: no

tag: batch

tag: 3' adapter

value: 17 (TACTCTCGTATGCCGTCTTCTGCTTG)

tag: read count

tag: read depth


title: ZWU19PlasmaB55A9

value: B10 (Day 29, 4 Hours After Lunch)

value: postL (Postprandial Late, 4 Hours After Lunch)

value: 9 (TCTCCTCGTATGCCGTCTTCTGCTTG)


title: MCECs, high glucose

description: Pooling : HG 1 + HG 2 + HG 3 = HG

source_name: mouse penis cavernous primary endothelial cell

tag: strain

tag: tissue


title: Normal_Cart_7_3

description:
normalized_counts.xlsx
raw_counts.xlsx

tag: age

tag: Sex

tag: oa grade


title: Normal_Cart_9_7

description:
normalized_counts.xlsx
raw_counts.xlsx


title: ZWU19PlasmaB57A22

tag: condition

tag: anatomical location

tag: rna source

tag: rna isolation kit

value: B6 (Day 15, 1 Hour After Lunch)

value: unknown

value: 22 (TTGTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB57A14

tag: shortid

tag: individual

tag: time point

value: B13 (Day 43, 4 Hours After Lunch)

tag: nutrition state

value: 14 (TATCATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB57A3

value: Healthy Subject

value: B4 (Day 1, 4 Hours After Lunch)

tag: female hormonal cycle phase

tag: female hormonal cycle length

tag: replicate

tag: batch

value: 3 (TCCACTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB52A19

value: Entire cardiovascular system

value: total cell-free biofluid RNA

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: B12 (Day 43, 1 Hour After Lunch)

tag: 3' adapter

value: 19 (TTATTTCGTATGCCGTCTTCTGCTTG)

tag: read count

tag: read depth


title: ZWU19SerumB61A24

source_name: Serum

value: B

value: 24 (TGCCATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB51A4

value: B2 (Day 1, Morning)

value: pre (Preprandial, Before Lunch)

value: no

value: 4 (TCCGTTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB61A14

value: postL (Postprandial Late, 4 Hours After Lunch)


title: ZWU19PlasmaB53A21

value: B11 (Day 43, Morning)

value: 21 (TTGACTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB57A12

value: Healthy Subject

value: B13 (Day 43, 4 Hours After Lunch)

value: 12 (TAATATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB54A7

value: Entire cardiovascular system

value: B6 (Day 15, 1 Hour After Lunch)

value: postE (Postprandial Early, 1 Hour After Lunch)

value: 7 (TCGCGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB57A7

value: total cell-free biofluid RNA

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.


title: ZWU19PlasmaB58A2

value: B5 (Day 15, Morning)

value: 2 (TCATCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB53A11

value: pre (Preprandial, Before Lunch)

value: 11 (TAACGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB57A16

value: B12 (Day 43, 1 Hour After Lunch)

value: L

value: 16 (TTACATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB51A8

value: B9 (Day 29, 1 Hour After Lunch)

value: no

value: 8 (TCTAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB57A23

value: Healthy Subject

value: B2 (Day 1, Morning)

value: 23 (TGTTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB61A18

value: Entire cardiovascular system

value: B4 (Day 1, 4 Hours After Lunch)

value: postL (Postprandial Late, 4 Hours After Lunch)

value: F

value: unknown

value: 18 (TAGCCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB54A1

value: total cell-free biofluid RNA

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: B11 (Day 43, Morning)

value: pre (Preprandial, Before Lunch)

value: 1 (TCACTTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB59A20

value: B9 (Day 29, 1 Hour After Lunch)

value: postE (Postprandial Early, 1 Hour After Lunch)

value: 20 (TTCATTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB59A1

value: Healthy Subject

value: L

value: no


title: ZWU19SerumB60A5

value: Entire cardiovascular system

value: B10 (Day 29, 4 Hours After Lunch)

value: 5 (TCCTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB53A23

value: B7 (Day 15, 4 Hours After Lunch)

value: postL (Postprandial Late, 4 Hours After Lunch)

value: 23 (TGTTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB60A9

value: total cell-free biofluid RNA

value: B12 (Day 43, 1 Hour After Lunch)

value: B

value: 9 (TCTCCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB61A16

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: 16 (TTACATCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB61A20

value: B4 (Day 1, 4 Hours After Lunch)


title: ZWU19PlasmaB56A11

value: B8 (Day 29, Morning)

value: pre (Preprandial, Before Lunch)

value: 11 (TAACGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB56A15

value: Healthy Subject

value: B6 (Day 15, 1 Hour After Lunch)

value: postE (Postprandial Early, 1 Hour After Lunch)

value: 15 (TGATGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB53A13

value: Entire cardiovascular system

value: B13 (Day 43, 4 Hours After Lunch)

value: no

value: 13 (TAGAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB56A21

value: total cell-free biofluid RNA

value: 21 (TTGACTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB52A18

value: B3 (Day 1, 1 Hour After Lunch)

value: B

value: 18 (TAGCCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB56A7

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: B11 (Day 43, Morning)

value: 7 (TCGCGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB56A23

value: pre (Preprandial, Before Lunch)

value: 23 (TGTTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB61A10

value: Healthy Subject

value: postL (Postprandial Late, 4 Hours After Lunch)

value: 10 (TTAAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB57A2

value: Entire cardiovascular system

value: postE (Postprandial Early, 1 Hour After Lunch)

value: 2 (TCATCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB102A14

value: NewTP (Approximately One Year After Day 1)

value: no

value: 14 (TATCATCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB60A1

value: total cell-free biofluid RNA

value: B8 (Day 29, Morning)

value: 1 (TCACTTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB53A10

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: B4 (Day 1, 4 Hours After Lunch)


title: ZWU19SerumB62A5

value: 5 (TCCTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB54A3

value: B6 (Day 15, 1 Hour After Lunch)

value: 3 (TCCACTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB52A22

value: Healthy Subject

value: B11 (Day 43, Morning)

value: pre (Preprandial, Before Lunch)

value: 22 (TTGTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB60A15

value: Entire cardiovascular system

value: B5 (Day 15, Morning)

value: L

value: 15 (TGATGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB58A17

value: B2 (Day 1, Morning)

value: F

value: unknown

value: no

value: 17 (TACTCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB102A16

value: total cell-free biofluid RNA

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: B9 (Day 29, 1 Hour After Lunch)

value: postE (Postprandial Early, 1 Hour After Lunch)

value: 16 (TTACATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB57A6

value: Healthy Subject

value: B6 (Day 15, 1 Hour After Lunch)

value: B

value: 6 (TCGATTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB62A9

value: Entire cardiovascular system

value: 9 (TCTCCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB102A4

value: B12 (Day 43, 1 Hour After Lunch)

value: 4 (TCCGTTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB52A20

value: F

value: 20 (TTCATTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB59A5

value: 5 (TCCTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB52A12

value: B8 (Day 29, Morning)

value: pre (Preprandial, Before Lunch)

value: no

value: 12 (TAATATCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB60A19

value: total cell-free biofluid RNA

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: B2 (Day 1, Morning)

value: postL (Postprandial Late, 4 Hours After Lunch)

value: 19 (TTATTTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB102A8

value: Healthy Subject

value: B4 (Day 1, 4 Hours After Lunch)

value: 8 (TCTAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB53A1

value: Entire cardiovascular system

value: 1 (TCACTTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB52A14

value: 14 (TATCATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB55A22

value: B7 (Day 15, 4 Hours After Lunch)

value: 22 (TTGTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB52A24

value: 24 (TGCCATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB53A5

value: L

value: 5 (TCCTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB60A17

value: B12 (Day 43, 1 Hour After Lunch)

value: postE (Postprandial Early, 1 Hour After Lunch)

value: no

value: 17 (TACTCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB60A21

value: total cell-free biofluid RNA

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: B3 (Day 1, 1 Hour After Lunch)

value: B

value: 21 (TTGACTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB52A10

value: Healthy Subject

value: Entire cardiovascular system

value: B11 (Day 43, Morning)

value: pre (Preprandial, Before Lunch)

value: ND

value: 10 (TTAAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB61A7

value: B8 (Day 29, Morning)

value: F

value: 7 (TCGCGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB59A4

value: B2 (Day 1, Morning)

value: postL (Postprandial Late, 4 Hours After Lunch)

value: 4 (TCCGTTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB102A10

value: total cell-free biofluid RNA

value: B9 (Day 29, 1 Hour After Lunch)

value: postE (Postprandial Early, 1 Hour After Lunch)

value: no


title: MCECs, high glucose+DKK2

description: Pooling : (HG+DKK2) 1 + (HG+DKK2) 2 + (HG+DKK2) 3 = HG+DKK2

tag: strain

tag: tissue

value: penis cavernous primary endothelial cell


title: ZWU19SerumB61A9

tag: condition

value: Healthy Subject

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: B12 (Day 43, 1 Hour After Lunch)

value: 9 (TCTCCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB58A4

tag: anatomical location

value: Entire cardiovascular system

value: B5 (Day 15, Morning)

value: pre (Preprandial, Before Lunch)


title: ZWU19PlasmaB53A3

tag: rna source

value: B4 (Day 1, 4 Hours After Lunch)

value: B

value: 3 (TCCACTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB56A6

tag: rna isolation kit

value: postL (Postprandial Late, 4 Hours After Lunch)

value: 6 (TCGATTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB53A7

value: total cell-free biofluid RNA

tag: shortid

value: B3 (Day 1, 1 Hour After Lunch)

value: 7 (TCGCGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB60A11

tag: individual

value: B9 (Day 29, 1 Hour After Lunch)

value: postE (Postprandial Early, 1 Hour After Lunch)

value: F

value: 11 (TAACGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB51A19

value: Healthy Subject

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

tag: time point

value: B10 (Day 29, 4 Hours After Lunch)

value: no

value: 19 (TTATTTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB55A16

value: Entire cardiovascular system

value: B13 (Day 43, 4 Hours After Lunch)

tag: nutrition state

value: L

value: unknown

value: 16 (TTACATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB54A18

value: total cell-free biofluid RNA

value: B5 (Day 15, Morning)

value: pre (Preprandial, Before Lunch)

tag: female hormonal cycle phase

value: 18 (TAGCCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB54A14

value: B7 (Day 15, 4 Hours After Lunch)

value: postL (Postprandial Late, 4 Hours After Lunch)

tag: female hormonal cycle length

value: 14 (TATCATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB57A18

value: Healthy Subject

value: B9 (Day 29, 1 Hour After Lunch)

value: postE (Postprandial Early, 1 Hour After Lunch)

tag: replicate


title: ZWU19PlasmaB58A16

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: B12 (Day 43, 1 Hour After Lunch)

value: B

value: no

tag: batch


title: ZWU19PlasmaB58A8

value: Entire cardiovascular system

value: B3 (Day 1, 1 Hour After Lunch)

value: F

tag: 3' adapter

value: 8 (TCTAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB60A18

value: total cell-free biofluid RNA

value: L

tag: read count


title: ZWU19SerumB59A22

value: 22 (TTGTATCGTATGCCGTCTTCTGCTTG)

tag: read depth


title: ZWU19SerumB61A3

value: B13 (Day 43, 4 Hours After Lunch)

value: 3 (TCCACTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB58A20

value: B10 (Day 29, 4 Hours After Lunch)

value: postL (Postprandial Late, 4 Hours After Lunch)

value: 20 (TTCATTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB58A6

value: Healthy Subject

value: B5 (Day 15, Morning)

value: pre (Preprandial, Before Lunch)

value: 6 (TCGATTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB52A5

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: B8 (Day 29, Morning)

value: no

value: 5 (TCCTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB58A10

value: Entire cardiovascular system

value: 10 (TTAAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB55A12

value: total cell-free biofluid RNA

value: B3 (Day 1, 1 Hour After Lunch)

value: postE (Postprandial Early, 1 Hour After Lunch)

value: 12 (TAATATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB53A15

value: 15 (TGATGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB62A23

value: B11 (Day 43, Morning)

value: L

value: 23 (TGTTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB51A13

value: Healthy Subject

value: 13 (TAGAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB102A20

value: B6 (Day 15, 1 Hour After Lunch)

value: 20 (TTCATTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB54A23

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: B4 (Day 1, 4 Hours After Lunch)

value: postL (Postprandial Late, 4 Hours After Lunch)

value: F


title: ZWU19PlasmaB51A23

value: Entire cardiovascular system

value: B2 (Day 1, Morning)

value: pre (Preprandial, Before Lunch)

value: no


title: ZWU19PlasmaB52A9

value: total cell-free biofluid RNA

value: B13 (Day 43, 4 Hours After Lunch)

value: 9 (TCTCCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB54A17

value: B12 (Day 43, 1 Hour After Lunch)

value: postE (Postprandial Early, 1 Hour After Lunch)

value: 17 (TACTCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB59A24

value: Healthy Subject

value: B5 (Day 15, Morning)

value: L

value: unknown

value: 24 (TGCCATCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB62A10

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: 10 (TTAAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB59A16

value: Entire cardiovascular system

value: B8 (Day 29, Morning)

value: F

value: 16 (TTACATCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB62A20

value: total cell-free biofluid RNA

value: B7 (Day 15, 4 Hours After Lunch)

value: postL (Postprandial Late, 4 Hours After Lunch)

value: no

value: 20 (TTCATTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB54A13

value: pre (Preprandial, Before Lunch)

value: 13 (TAGAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB57A8

value: Healthy Subject

value: 8 (TCTAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB62A22

value: B3 (Day 1, 1 Hour After Lunch)

value: postE (Postprandial Early, 1 Hour After Lunch)

value: L

value: 22 (TTGTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB52A1

value: Entire cardiovascular system

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: 1 (TCACTTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB54A9

value: B8 (Day 29, Morning)

value: 9 (TCTCCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB55A4

value: total cell-free biofluid RNA

value: 4 (TCCGTTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB54A5

value: B9 (Day 29, 1 Hour After Lunch)

value: F

value: 5 (TCCTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB59A9

value: B7 (Day 15, 4 Hours After Lunch)

value: postL (Postprandial Late, 4 Hours After Lunch)

value: B

value: no


title: ZWU19PlasmaB57A21

value: Healthy Subject

value: postE (Postprandial Early, 1 Hour After Lunch)

value: 21 (TTGACTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB62A14

value: Entire cardiovascular system

value: B5 (Day 15, Morning)

value: pre (Preprandial, Before Lunch)

value: 14 (TATCATCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB59A18

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: B4 (Day 1, 4 Hours After Lunch)

value: 18 (TAGCCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB60A3

value: total cell-free biofluid RNA

value: B2 (Day 1, Morning)

value: L

value: 3 (TCCACTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB59A12

value: B8 (Day 29, Morning)

value: 12 (TAATATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB58A22

value: B9 (Day 29, 1 Hour After Lunch)

value: no

value: 22 (TTGTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB61A2

value: Healthy Subject

value: B7 (Day 15, 4 Hours After Lunch)

value: postL (Postprandial Late, 4 Hours After Lunch)

value: unknown

value: 2 (TCATCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB56A19

value: Entire cardiovascular system

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: 19 (TTATTTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB62A24

value: total cell-free biofluid RNA

value: 24 (TGCCATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB53A19

value: B6 (Day 15, 1 Hour After Lunch)

value: postE (Postprandial Early, 1 Hour After Lunch)


title: ZWU19SerumB62A12

value: B10 (Day 29, 4 Hours After Lunch)

value: L


title: ZWU19PlasmaB51A9

value: 9 (TCTCCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB51A2

value: B5 (Day 15, Morning)

value: pre (Preprandial, Before Lunch)

value: no


title: ZWU19PlasmaB53A24

value: Healthy Subject

value: B12 (Day 43, 1 Hour After Lunch)


title: ZWU19SerumB62A16

value: B11 (Day 43, Morning)

value: 16 (TTACATCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB60A7

value: Entire cardiovascular system

value: B13 (Day 43, 4 Hours After Lunch)

value: postL (Postprandial Late, 4 Hours After Lunch)

value: 7 (TCGCGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB57A11

value: total cell-free biofluid RNA

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: 11 (TAACGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB55A18

value: B9 (Day 29, 1 Hour After Lunch)

value: postE (Postprandial Early, 1 Hour After Lunch)

value: 18 (TAGCCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB61A21

value: B5 (Day 15, Morning)

value: B

value: 21 (TTGACTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB52A16

value: Healthy Subject

value: B4 (Day 1, 4 Hours After Lunch)

value: no


title: ZWU19PlasmaB56A13

value: B12 (Day 43, 1 Hour After Lunch)

value: 13 (TAGAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB62A3

value: Entire cardiovascular system

value: B10 (Day 29, 4 Hours After Lunch)

value: F

value: unknown

value: 3 (TCCACTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB53A18

value: total cell-free biofluid RNA

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: B7 (Day 15, 4 Hours After Lunch)

value: postL (Postprandial Late, 4 Hours After Lunch)

value: L


title: ZWU19PlasmaB53A14

value: B13 (Day 43, 4 Hours After Lunch)

value: 14 (TATCATCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB61A11

value: Healthy Subject

value: 11 (TAACGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB54A4

value: 4 (TCCGTTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB61A15

value: B12 (Day 43, 1 Hour After Lunch)

value: postE (Postprandial Early, 1 Hour After Lunch)

value: no

value: 15 (TGATGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB61A23

value: Entire cardiovascular system

value: B2 (Day 1, Morning)

value: pre (Preprandial, Before Lunch)

value: F

value: 23 (TGTTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB51A1

value: total cell-free biofluid RNA

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: 1 (TCACTTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB57A4


title: ZWU19PlasmaB53A9

value: B10 (Day 29, 4 Hours After Lunch)

value: postL (Postprandial Late, 4 Hours After Lunch)

value: L

value: 9 (TCTCCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB102A24

value: Healthy Subject

value: B3 (Day 1, 1 Hour After Lunch)

value: 24 (TGCCATCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB62A13

value: B8 (Day 29, Morning)

value: unknown

value: no

value: 13 (TAGAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB51A5

value: Entire cardiovascular system

value: B11 (Day 43, Morning)

value: pre (Preprandial, Before Lunch)

value: 5 (TCCTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB55A20

value: total cell-free biofluid RNA

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: B9 (Day 29, 1 Hour After Lunch)

value: postE (Postprandial Early, 1 Hour After Lunch)

value: F

value: 20 (TTCATTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB59A8

value: Healthy Subject

value: 8 (TCTAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB55A24

value: B13 (Day 43, 4 Hours After Lunch)

value: postL (Postprandial Late, 4 Hours After Lunch)

value: L

value: 24 (TGCCATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB54A8

value: B7 (Day 15, 4 Hours After Lunch)


title: ZWU19PlasmaB56A22

value: Entire cardiovascular system

value: B2 (Day 1, Morning)

value: no

value: 22 (TTGTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB102A6

value: total cell-free biofluid RNA

value: B3 (Day 1, 1 Hour After Lunch)

value: 6 (TCGATTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB102A21

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: 21 (TTGACTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB61A13

value: B4 (Day 1, 4 Hours After Lunch)

value: 13 (TAGAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB59A2

value: Healthy Subject

value: B11 (Day 43, Morning)

value: pre (Preprandial, Before Lunch)

value: 2 (TCATCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB51A3

value: postE (Postprandial Early, 1 Hour After Lunch)

value: L

value: 3 (TCCACTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB59A6

value: Entire cardiovascular system

value: postL (Postprandial Late, 4 Hours After Lunch)


title: ZWU19PlasmaB102A2

value: B10 (Day 29, 4 Hours After Lunch)

value: no


title: ZWU19PlasmaB55A10

value: total cell-free biofluid RNA

value: B13 (Day 43, 4 Hours After Lunch)

value: 10 (TTAAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB62A4

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: B5 (Day 15, Morning)

value: 4 (TCCGTTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB56A12

value: Healthy Subject

value: B12 (Day 43, 1 Hour After Lunch)

value: unknown

value: 12 (TAATATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB56A4

value: B8 (Day 29, Morning)

value: pre (Preprandial, Before Lunch)


title: ZWU19PlasmaB51A21

value: 21 (TTGACTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB60A22

value: Entire cardiovascular system

value: 22 (TTGTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB56A8

value: B7 (Day 15, 4 Hours After Lunch)

value: postL (Postprandial Late, 4 Hours After Lunch)

value: B

value: 8 (TCTAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB102A23

value: total cell-free biofluid RNA

value: B6 (Day 15, 1 Hour After Lunch)

value: postE (Postprandial Early, 1 Hour After Lunch)

value: no

value: 23 (TGTTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB62A2

value: Healthy Subject

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: B5 (Day 15, Morning)

value: 2 (TCATCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB102A15

value: 15 (TGATGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB60A12

value: 12 (TAATATCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB61A17

value: Entire cardiovascular system

value: pre (Preprandial, Before Lunch)

value: 17 (TACTCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB58A14

value: B10 (Day 29, 4 Hours After Lunch)

value: postL (Postprandial Late, 4 Hours After Lunch)

value: L

value: 14 (TATCATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB54A19

value: total cell-free biofluid RNA

value: F

value: 19 (TTATTTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB62A6

value: 6 (TCGATTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB102A5

value: Healthy Subject

value: B6 (Day 15, 1 Hour After Lunch)

value: postE (Postprandial Early, 1 Hour After Lunch)

value: no

value: 5 (TCCTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB102A13

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: B4 (Day 1, 4 Hours After Lunch)

value: 13 (TAGAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB51A11

value: Entire cardiovascular system

value: B5 (Day 15, Morning)

value: pre (Preprandial, Before Lunch)

value: 11 (TAACGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB60A16

value: B10 (Day 29, 4 Hours After Lunch)

value: postL (Postprandial Late, 4 Hours After Lunch)

value: 16 (TTACATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB102A1

value: total cell-free biofluid RNA

value: B13 (Day 43, 4 Hours After Lunch)

value: 1 (TCACTTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB102A11

value: Healthy Subject


title: ZWU19PlasmaB58A24

value: B8 (Day 29, Morning)

value: F

value: 24 (TGCCATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB55A6

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: unknown

value: no

value: 6 (TCGATTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB58A18

value: Entire cardiovascular system

value: B6 (Day 15, 1 Hour After Lunch)

value: postE (Postprandial Early, 1 Hour After Lunch)

value: 18 (TAGCCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB54A21

value: L

value: 21 (TTGACTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB51A17

value: total cell-free biofluid RNA

value: B3 (Day 1, 1 Hour After Lunch)

value: 17 (TACTCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB55A14

value: Healthy Subject

value: B7 (Day 15, 4 Hours After Lunch)

value: postL (Postprandial Late, 4 Hours After Lunch)

value: 14 (TATCATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB102A17

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: B13 (Day 43, 4 Hours After Lunch)

value: B


title: ZWU19PlasmaB52A7

value: no

value: 7 (TCGCGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB55A23

value: Entire cardiovascular system

value: B6 (Day 15, 1 Hour After Lunch)

value: 23 (TGTTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB61A4

value: 4 (TCCGTTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB53A2

value: B10 (Day 29, 4 Hours After Lunch)

value: F

value: 2 (TCATCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB61A8

value: total cell-free biofluid RNA

value: B12 (Day 43, 1 Hour After Lunch)

value: postE (Postprandial Early, 1 Hour After Lunch)

value: L

value: 8 (TCTAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB59A10

value: Healthy Subject

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: B9 (Day 29, 1 Hour After Lunch)

value: 10 (TTAAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB54A11

value: no

value: 11 (TAACGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB57A13

value: Entire cardiovascular system

value: B7 (Day 15, 4 Hours After Lunch)

value: postL (Postprandial Late, 4 Hours After Lunch)

value: 13 (TAGAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB59A14

value: 14 (TATCATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB54A15

value: 15 (TGATGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB52A3

value: total cell-free biofluid RNA

value: B11 (Day 43, Morning)

value: pre (Preprandial, Before Lunch)

value: 3 (TCCACTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB51A20

value: L

value: 20 (TTCATTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB55A8

value: Healthy Subject

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: unknown

value: 8 (TCTAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB58A1

value: B4 (Day 1, 4 Hours After Lunch)

value: B

value: 1 (TCACTTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB57A17

value: Entire cardiovascular system

value: postL (Postprandial Late, 4 Hours After Lunch)

value: no

value: 17 (TACTCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB61A6

value: total cell-free biofluid RNA

value: B6 (Day 15, 1 Hour After Lunch)

value: postE (Postprandial Early, 1 Hour After Lunch)

value: 6 (TCGATTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB55A13

value: B10 (Day 29, 4 Hours After Lunch)

value: F

value: 13 (TAGAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB55A2

value: Healthy Subject

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: 2 (TCATCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB58A5

value: B9 (Day 29, 1 Hour After Lunch)

value: unknown

value: 5 (TCCTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB57A19

value: Entire cardiovascular system

value: B3 (Day 1, 1 Hour After Lunch)

value: B

value: 19 (TTATTTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB56A17

value: total cell-free biofluid RNA

value: postL (Postprandial Late, 4 Hours After Lunch)

value: 17 (TACTCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB53A22

value: B2 (Day 1, Morning)

value: pre (Preprandial, Before Lunch)

value: 22 (TTGTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB52A2

value: postE (Postprandial Early, 1 Hour After Lunch)

value: no


title: ZWU19PlasmaB58A3

value: Healthy Subject

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: 3 (TCCACTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB51A10

value: B9 (Day 29, 1 Hour After Lunch)

value: 10 (TTAAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB54A20

value: Entire cardiovascular system

value: 20 (TTCATTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB51A7

value: B10 (Day 29, 4 Hours After Lunch)

value: 7 (TCGCGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB56A24

value: total cell-free biofluid RNA

value: B4 (Day 1, 4 Hours After Lunch)

value: 24 (TGCCATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB54A24

value: postL (Postprandial Late, 4 Hours After Lunch)


title: ZWU19PlasmaB58A7

value: B5 (Day 15, Morning)

value: pre (Preprandial, Before Lunch)

value: L


title: ZWU19SerumB62A18

value: B8 (Day 29, Morning)

value: 18 (TAGCCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB59A23

value: Healthy Subject

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: B12 (Day 43, 1 Hour After Lunch)

value: postE (Postprandial Early, 1 Hour After Lunch)

value: 23 (TGTTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB52A6

value: Entire cardiovascular system

value: F

value: no

value: 6 (TCGATTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB53A16

value: total cell-free biofluid RNA

value: 16 (TTACATCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB60A8

value: B6 (Day 15, 1 Hour After Lunch)

value: 8 (TCTAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB53A12

value: B2 (Day 1, Morning)

value: pre (Preprandial, Before Lunch)

value: B

value: 12 (TAATATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB57A15

value: Healthy Subject

value: B5 (Day 15, Morning)

value: 15 (TGATGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB56A18

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: B7 (Day 15, 4 Hours After Lunch)

value: postL (Postprandial Late, 4 Hours After Lunch)

value: L

value: 18 (TAGCCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB62A17

value: Entire cardiovascular system

value: B13 (Day 43, 4 Hours After Lunch)

value: 17 (TACTCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB59A13

value: total cell-free biofluid RNA

value: B3 (Day 1, 1 Hour After Lunch)

value: postE (Postprandial Early, 1 Hour After Lunch)

value: no

value: 13 (TAGAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB62A21

value: 21 (TTGACTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB60A2

value: Healthy Subject

value: B

value: 2 (TCATCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB57A5

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: B5 (Day 15, Morning)

value: pre (Preprandial, Before Lunch)

value: 5 (TCCTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB56A14

value: B11 (Day 43, Morning)

value: L

value: 14 (TATCATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB52A21

value: Entire cardiovascular system

value: B13 (Day 43, 4 Hours After Lunch)

value: postL (Postprandial Late, 4 Hours After Lunch)


title: ZWU19PlasmaB54A6

value: total cell-free biofluid RNA

value: 6 (TCGATTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB52A15

value: no

value: 15 (TGATGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB60A4

value: Healthy Subject

value: B6 (Day 15, 1 Hour After Lunch)

value: postE (Postprandial Early, 1 Hour After Lunch)

value: 4 (TCCGTTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB62A11

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: B10 (Day 29, 4 Hours After Lunch)

value: F

value: 11 (TAACGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB52A11


title: ZWU19PlasmaB57A9

value: B4 (Day 1, 4 Hours After Lunch)

value: 9 (TCTCCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB56A20

value: Entire cardiovascular system

value: 20 (TTCATTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB55A1

value: postL (Postprandial Late, 4 Hours After Lunch)

value: unknown

value: 1 (TCACTTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB56A16

value: total cell-free biofluid RNA

value: B3 (Day 1, 1 Hour After Lunch)

value: L

value: no

value: 16 (TTACATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB54A2

value: Healthy Subject

value: B

value: 2 (TCATCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB51A6

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: B6 (Day 15, 1 Hour After Lunch)

value: postE (Postprandial Early, 1 Hour After Lunch)

value: 6 (TCGATTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB62A8

value: Entire cardiovascular system

value: 8 (TCTAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB52A23

value: B12 (Day 43, 1 Hour After Lunch)

value: ND

value: 23 (TGTTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB102A19

value: total cell-free biofluid RNA

value: 19 (TTATTTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB53A4

value: L

value: 4 (TCCGTTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB61A19

value: B2 (Day 1, Morning)

value: pre (Preprandial, Before Lunch)

value: no


title: ZWU19PlasmaB56A10

value: Healthy Subject

value: B3 (Day 1, 1 Hour After Lunch)

value: B

value: 10 (TTAAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB52A13

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: B4 (Day 1, 4 Hours After Lunch)

value: postL (Postprandial Late, 4 Hours After Lunch)

value: 13 (TAGAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB60A20

value: Entire cardiovascular system

value: 20 (TTCATTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB102A7

value: total cell-free biofluid RNA

value: NewTP (Approximately One Year After Day 1)

value: postE (Postprandial Early, 1 Hour After Lunch)

value: 7 (TCGCGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB59A3

value: B11 (Day 43, Morning)

value: 3 (TCCACTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB52A17

value: B8 (Day 29, Morning)

value: pre (Preprandial, Before Lunch)

value: F

value: 17 (TACTCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB60A24

value: Healthy Subject

value: B2 (Day 1, Morning)

value: B

value: 24 (TGCCATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB57A1

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: no

value: 1 (TCACTTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB53A8

value: Entire cardiovascular system

value: total cell-free biofluid RNA

value: B5 (Day 15, Morning)

value: 8 (TCTAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB55A19

value: 19 (TTATTTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB61A22

value: B13 (Day 43, 4 Hours After Lunch)

value: postL (Postprandial Late, 4 Hours After Lunch)

value: L

value: 22 (TTGTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB59A7

value: B3 (Day 1, 1 Hour After Lunch)

value: postE (Postprandial Early, 1 Hour After Lunch)

value: 7 (TCGCGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB102A9

value: Healthy Subject

value: B12 (Day 43, 1 Hour After Lunch)

value: unknown

value: 9 (TCTCCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB55A15

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: pre (Preprandial, Before Lunch)

value: no

value: 15 (TGATGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB53A6

value: Entire cardiovascular system

value: total cell-free biofluid RNA

value: 6 (TCGATTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB60A10

value: B4 (Day 1, 4 Hours After Lunch)

value: postL (Postprandial Late, 4 Hours After Lunch)

value: 10 (TTAAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB58A12

value: B2 (Day 1, Morning)

value: B

value: 12 (TAATATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB56A9

value: Healthy Subject

value: postE (Postprandial Early, 1 Hour After Lunch)


title: ZWU19PlasmaB51A22

value: B12 (Day 43, 1 Hour After Lunch)

value: 22 (TTGTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB60A14

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: 14 (TATCATCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB62A7

value: B8 (Day 29, Morning)

value: pre (Preprandial, Before Lunch)

value: no

value: 7 (TCGCGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB61A12

value: Entire cardiovascular system

value: total cell-free biofluid RNA

value: B9 (Day 29, 1 Hour After Lunch)


title: ZWU19PlasmaB55A17

value: B7 (Day 15, 4 Hours After Lunch)

value: postL (Postprandial Late, 4 Hours After Lunch)

value: 17 (TACTCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB102A3

value: 3 (TCCACTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB55A21

value: Healthy Subject

value: L

value: 21 (TTGACTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB56A3

value: B10 (Day 29, 4 Hours After Lunch)


title: ZWU19SerumB62A1

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: B6 (Day 15, 1 Hour After Lunch)

value: postE (Postprandial Early, 1 Hour After Lunch)

value: B

value: 1 (TCACTTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB58A19

value: F

value: 19 (TTATTTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB51A16

value: Entire cardiovascular system

value: total cell-free biofluid RNA

value: 16 (TTACATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB102A22

value: B7 (Day 15, 4 Hours After Lunch)

value: postL (Postprandial Late, 4 Hours After Lunch)

value: no

value: 22 (TTGTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB55A11

value: Healthy Subject

value: B13 (Day 43, 4 Hours After Lunch)

value: 11 (TAACGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB58A9

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: B3 (Day 1, 1 Hour After Lunch)

value: 9 (TCTCCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB51A14

value: B2 (Day 1, Morning)

value: pre (Preprandial, Before Lunch)

value: 14 (TATCATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB56A5

value: B11 (Day 43, Morning)

value: L

value: 5 (TCCTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB51A18

value: Entire cardiovascular system

value: total cell-free biofluid RNA

value: B8 (Day 29, Morning)

value: F

value: 18 (TAGCCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB58A13

value: Healthy Subject

value: no

value: 13 (TAGAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB51A12

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: B5 (Day 15, Morning)

value: 12 (TAATATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB58A21

value: B9 (Day 29, 1 Hour After Lunch)

value: postE (Postprandial Early, 1 Hour After Lunch)

value: 21 (TTGACTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB60A23

value: B11 (Day 43, Morning)

value: pre (Preprandial, Before Lunch)

value: 23 (TGTTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB59A15

value: Entire cardiovascular system

value: B3 (Day 1, 1 Hour After Lunch)

value: unknown

value: 15 (TGATGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB102A12

value: total cell-free biofluid RNA

value: B4 (Day 1, 4 Hours After Lunch)

value: postL (Postprandial Late, 4 Hours After Lunch)


title: ZWU19PlasmaB51A24

value: Healthy Subject

value: B2 (Day 1, Morning)

value: L

value: no

value: 24 (TGCCATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB58A11

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: B12 (Day 43, 1 Hour After Lunch)

value: postE (Postprandial Early, 1 Hour After Lunch)

value: 11 (TAACGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB102A18

value: B8 (Day 29, Morning)

value: pre (Preprandial, Before Lunch)

value: 18 (TAGCCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB59A19

value: Entire cardiovascular system

value: B6 (Day 15, 1 Hour After Lunch)

value: 19 (TTATTTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB58A23

value: total cell-free biofluid RNA

value: 23 (TGTTATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB58A15

value: Healthy Subject

value: B13 (Day 43, 4 Hours After Lunch)

value: postL (Postprandial Late, 4 Hours After Lunch)

value: 15 (TGATGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB53A20

value: B9 (Day 29, 1 Hour After Lunch)

value: F

value: 20 (TTCATTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB60A13

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: B11 (Day 43, Morning)

value: L

value: unknown

value: no

value: 13 (TAGAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB54A10

value: Entire cardiovascular system

value: B3 (Day 1, 1 Hour After Lunch)

value: postE (Postprandial Early, 1 Hour After Lunch)

value: 10 (TTAAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB52A8

value: Healthy Subject

value: total cell-free biofluid RNA

value: ND

value: 8 (TCTAGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB57A20

value: B6 (Day 15, 1 Hour After Lunch)


title: ZWU19PlasmaB56A1

value: pre (Preprandial, Before Lunch)

value: 1 (TCACTTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB55A3

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: B9 (Day 29, 1 Hour After Lunch)

value: F

value: 3 (TCCACTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB54A16

value: B8 (Day 29, Morning)

value: L

value: no

value: 16 (TTACATCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB52A4

value: Entire cardiovascular system

value: postE (Postprandial Early, 1 Hour After Lunch)

value: 4 (TCCGTTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB60A6

value: Healthy Subject

value: total cell-free biofluid RNA

value: B12 (Day 43, 1 Hour After Lunch)

value: 6 (TCGATTCGTATGCCGTCTTCTGCTTG)


title: thymus_exEC

city: Seattle

first: Kirsten

last: Cooper

state: WA

address: 1100 Fairview Ave. N, Rm S3-208

institute: Fred Hutchinson Cancer Research Center

description: Gene expression from exEC derived from thymus

hyb_protocol: 10 ug of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.

scan_protocol: Affymetrix GeneArray scanner 7G

data_processing: Feature intensity was extracted by Affymetrix GeneChip Command Console as CEL files. Used Bioconductor Affy for normalization (GC-RMA) and gene annotation

label: Biotin

source_name: exECs harvested from mouse thymus

label_protocol: 500 ng total RNA converted to cRNA using Affymetrix GeneChip®3' IVT Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).

growth_protocol: To propigate exECs, cell culture medium containing murine recombinant VEGF (50ng/ml) and FGF-2 (100ng/ml), endothelial growth supplement (bovine hypothalamus) (Alfa Aesar), sb431542 (Tocris Biosciences), heparin (50g/ml) (Sigma-Aldrich), 1% Glutamax (Life Technologies), 1% non-essential amino acids (Life Technologies), 1% HEPES Buffer (Life Technologies), 1% antibiotic-antimycotic (Life Technologies) was replaced every 48h.

extract_protocol: RNA extraction plus kit was used to isolate RNA, and cDNA was made with Invitrogen Superscriptase.

treatment_protocol: Cells were first isolated using CD45 MACS cell depletion, then endothelial cells were FACS purified (CD45- EpCAM- VE-Cad+). To generate exECs, CD45-VE-Cadherin+ cells were FACS purified and incubated with lentivirus containing the E4ORF1 construct for 48 hours. To obtain sufficient RNA for every timepoint, thymic ECs of several mice were pooled.

tag: strain

tag: age

tag: gender

value: female

value: thymus

tag: cell type

value: ex-vivo grown endothelial cells, derived from thymus


title: ZWU19PlasmaB55A7

tag: condition

tag: anatomical location

tag: rna source

value: Self developed protocol, Tuschl_RNA_isolation_library_preparation_protocol.pdf; executed on epMotion 5075 liquid handling system.

value: B3 (Day 1, 1 Hour After Lunch)

value: 7 (TCGCGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB51A15

tag: rna isolation kit

tag: shortid

tag: individual

value: B6 (Day 15, 1 Hour After Lunch)

value: 15 (TGATGTCGTATGCCGTCTTCTGCTTG)


title: ZWU19PlasmaB56A2

tag: time point

value: B13 (Day 43, 4 Hours After Lunch)

tag: nutrition state

value: postL (Postprandial Late, 4 Hours After Lunch)

tag: female hormonal cycle phase

value: B

value: no

value: 2 (TCATCTCGTATGCCGTCTTCTGCTTG)


title: ZWU19SerumB59A17

value: Healthy Subject

value: Entire cardiovascular system

value: B11 (Day 43, Morning)

value: pre (Preprandial, Before Lunch)

value: L

tag: female hormonal cycle length

tag: replicate

tag: batch

value: 17 (TACTCTCGTATGCCGTCTTCTGCTTG)