title: dgriMo010_f_wb_R2.leftover

description: mixed-species RNA preparation


title: dgriG1_m_wb_R3.leftover

status: Public on Jun 01 2018

type: SRA

city: Bethesda

first: Brian

last: Oliver

state: MD

address: 50 South Drive

department: LBG

country: USA

institute: NIDDK, NIH

description: mixed-species RNA preparation

library_source: transcriptomic

data_processing:
We performed single-end 76 bp sequencing on the HiSeq2000 Sequencing System (Illumina, San Diego, CA) according to the manufacturer. De-multiplexed reads (Chastity > 0.6) FASTQ output was from Illumina CASAVA v.1.8.2. For each mixed sample, the RNA-seq reads were first mapped to a merged genome and annotation including D. grimshawi (dgri_caf1 assembly; Drosophila_grimshawi.GCA_000005155.1.29.dna_sm.genome.fa), D. melanogaster (FlyBase release 6 version 04 ; FlyBase file: dmel-all-chromosome-r6.04.fasta), and A. stephensi (Anopheles-stephensi-Indian_SCAFFOLDS_AsteI2.fa), appended with updated ERCC sequences (Jiang et al., Genome Research, 2011, PMID21816910; Lee et al., J Genomics, 2016, PMID27512518) using STAR (v2.4.2a) (Doblin et al., Bioinformatics, 2012, PMID23104886) with default parameters or HiSAT2 (v2.0.5; --dta and -max-intronlen = 300,000) (Kim et al., 2015, Nature Methods, PMID25751142). All processed data for Hawaiian species was based on alignment to D. grimshawi (FlyBase version 2016_05). For each chromosome or scaffold in the merged annotation, we add a four letter prefix to identify the species (e.g., scaffold_15110 => dgri_ scaffold_15110) for bam file parsing using SAMtools (v0.1.19) (Li et al., 2009, PMID19505943). We used a demultiplexer (http://github.com/haiwangyang/demultiplexer) to fetch the prefix of read names in the bam alignments and obtained all uniquely-mapped and multi-hit (with < 10 hits, >10 hits are excluded) reads (in bam format) that mapped exclusively to a single species or exclusively to ERCC. We reconverted bam files for each species to raw read fastq files using BEDTools (2.25.0; bamtofastq) (Quinlan and Hall, 2010, Bioinformatics, PMID20110278).
After this step, reads of each multi-species sample were split into three categories based on the mapped genome– D. grimshawi, ERCC, and leftover, which includes unmapped reads, reads mapped to multiple species (or multiple species plus ERCC), and reads mapped to either D. melanogaster or A. stephensi. For multi-species samples, the original fastq file can be restored by merging the reads of D. grimshawi as well as the ERCC and leftover reads. We provide both STAR (SRR3355*) and HiSAT2 (SRR724* & SRR725*) fastq files in this GEO entry. We provided associated gene-level read count files based on HiSAT2 alignments, and the read count files have both FlyBase version (*htseq_reverse.HiSAT2.FB.txt) and YO version (*htseq_reverse.HiSAT2.YO.txt) in Supplementary files of this GEO. For the latter one, we also provide two extra columns in the expression matrix, showing the maximum exon-level overlap (Jaccard index) between YO gene ID and FlyBase gene ID. In addition, we provided bigWig tracks for all samples (replicated merged), YO annotation files (in both gtf and gff3 format), ortholog look up table (among YO gene ID, FlyBase gene ID, and dmel gene), gene-level normalized read counts based on either YO or FlyBase annotation, and transcript-level transcripts per million (TPM) based on YO or FlyBase annotation.

molecule: polyA RNA

organism: Drosophila grimshawi

source_name: leftover of unmapped reads plus reads mapped to Drosophila melanogaster and Anopheles stephensi

growth_protocol: All lines were originated from a single wild-caught female (iso-female line) and were reared at the Hawaiian Drosophila Research Stock Center. For D. grimishawi, the G1 strain was isolated from the Auwahi dry forest of East Maui in July 1965, the ZA27Z2 and ZA27Z3 strains were isolated from the Makawao Forest Reserve of East Maui in March 2010, and Mo010 strain was isolated from the southeast of Kawela Gulch of Molokai Island in December 2010, and Wm1041 strain was isolated from the Iao Valley of West Maui in July 2014. For D. silvestris, Y11R6 strain was isolated from the Olaa Forest Reserve of Hawaii in December 1986, and U26B9 strain was isolated from the Kahuku Ranch of Hawaii in January 1997. The D. hemipeza strain W40B14 was isolated from the Palikea Ridge of Oahu in July 1983. The D. heteroneura W48B6 strain was isolated from the base of Hinakapoula of Hawaii in August 1983. The D. hawaiiensis Y17P5 strain was isolated from Kipuka Ki, Hawaii Volcano National Park in March 1987. 70-100 Flies were grown in gallon glass jars with a 5 cm bed of dry sterile sand. Flies were fed once a week with five vials containing ~3ml of Yeastless Wheeler Clayton medium (placed horizontally in each jar). A single batch of this media was prepared with: 16g each of agar, rice cereal, Kellogg’s Special K, and wheat germ powder, plus 400g Gerber’s banana baby food and 1400ml water. These ingredients were mixed, cooked to boil and cooled. After cooling (but still liquid), 6ml each of 95% ethanol and propionic acid was added and mixed and then used to fill glass dram vials, which were stored 4oC until needed. 1/5 sheet of folded non-bleached facial tissue paper saturated with a water extract of fermenting Clermontia sp. leaves was added to each vial. Females of each strain laid eggs onto the tissue. Vials with eggs were collected weekly. Eggs hatched in about one week. Larvae were fed weekly for 2 weeks (as needed based on density of larvae). The recipe for cornmeal paste larval food was: 800g cornmeal, 100g brewers yeast, 90g soybean meal, 90g agar, 133ml karo syrup, 45g molasses and 4L water. This mixture was boiled, cooled, and 45ml each of 95% ethanol and propionic acid was added. The cornmeal paste is mixed with the water extract of fermenting Clermontia sp. leaves. After 3 weeks the larval tubes were transferred to moist sterile sand jars. Larvae burrow into the sand for pupation. Adults eclosed after 6 weeks and were aged for dissection. Females of all sequenced species began laying eggs around 4 weeks. Males of some species tend to die much earlier than females, making it problematic to collect sexually mature flies of both sexes at the same age for dissection. All flies were grown at 18oC with a 12h/12h Day/Night cycle.

extract_protocol:
Flies were anesthetized by briefly chilling on a 0oC glass plate. All samples were dissected in Phosphate Buffered Saline solution pH7.4 and transferred to 112ul of RNA later (Life Technologies, Carlsbad, CA) in 96-well plates at room temperature after being macerated with forceps. After transport, plates were stored at -80oC. For D. grimshawi samples, we added 20-30 1.0mm glass beads (BioSpec Products, Bartlesville, OK) to each sample and added 600ul RLT buffer from the RNeasy 96 kit (Qiagen, Valencia, CA) to each well. We homogenized the samples three times for 60 seconds each with a mini-beadbeater (Biospec Products, Bartlesville, OK). Extraction of total RNA was performed using the RNeasy 96 kit according to manufacturer's guide (spin version), except that we used 712μl of 70% ethanol in each well to dilute the RNAlater and RLT buffer. D. grimshawi RNA was added to D. melanogaster RNA and A. stephensi embryos for homogenization and a second round of RNA extraction using the RNeasy kit. D. melanogaster and A. stephensi RNAseq will be reported elsewhere. Four head and gonad samples of Wm1041 were split into mixed-species and single-species RNA preparations. All other samples were prepared without mixing with other species using the same RNeasy protocol. RNA was quantified using the Quant-iT RiboGreen quantification kit Life Technologies, Carlsbad, CA).
We used the Truseq PolyA+ and stranded mRNA kit with half volumes (Illumina, San Diego, CA). 100ng total RNA was input for all samples. We also added 20pg Pool 78A or 78B ERCC spike-ins (Jiang et al., Genome Research, 2011, PMID 21816910) added to the Fragment-Prime-Finish Mix during mRNA fragmentation step. Libraries were quantified with Quant-iT PicoGreen (Life Technologies, Carlsbad, CA), and pooled for multiplexed sequencing.

tag: strain

tag: tissue

value: not applicable

tag: Sex

tag: replicate


title: dgriWm1041_f_hd_R1a.leftover

description: mixed-species RNA preparation


title: dgriG1_m_wb_R2.leftover

description: mixed-species RNA preparation


title: dgriG1_m_wb_R1.leftover

description: mixed-species RNA preparation


title: dgriMo010_m_wb_R3.leftover

description: mixed-species RNA preparation


title: 4T1 TAM 4

type: RNA

city: London

first: JONATHAN

last: CARON

state: Greater London

address: New Hunt's House, Newcomen St

department: Cancer Studies

country: United Kingdom

institute: KING'S COLLEGE LONDON

hyb_protocol: As recommended by the array manufacturer with the following exception: NuGEN recommended hybridization temperature of 48°C. Staining with Cy3-Streptavidin.

scan_protocol: Image BeadChip on the iScan System

data_processing: Raw fluorescence signals were collected using GenomeStudio (Illumina), and imported into Partek Genomics Suite (PGS) for further analysis. Background was subtracted from the raw data and fluorescence signals were normalized using the quantiles method.

label: biotin

molecule: total RNA

organism: Mus musculus

label_protocol: mRNA was converted to cDNA, amplified using the Ovation® PicoSL WTA system V2 (NuGEN), biotinylated using the Encore® BiotinIL Module (NuGEN)

extract_protocol: According to the manufacturers’ protocol, PureLink™ RNA Mini Kit

treatment_protocol: Miltenyi magnetic beads

tag: strain background

value: Balb/c

tag: gender

value: female

value: 4T1 tumor

tag: cell type

value: F4/80+ tumor-associated macrophages


title: 4T1 TAM 2


title: 4T1 TAM 3


title: dgriG1_m_vs_R3.leftover

description: mixed-species RNA preparation


title: M0.4

value: spleen from 4T1-bearing mouse

value: splenic-derived macrophage_MCSF_72h


title: M0.3


title: dgriG1_m_vs_R2.leftover

description: mixed-species RNA preparation


title: M0.1


title: M0.2


title: 4T1 TAM 1


title: F01D4.5(baf20) rep4

city: San Antonio

first: Alfred

middle: L

last: Fisher

state: TX

address: 7703 Floyd Curl Dr

institute: University of Texas Health Science Center

data_processing:
Demultiplexing with CASAVA was employed to generate the fastq file for each sample
fastq files were uploaded to the Galaxy server for analysis
Sequencing reads were aligned to the C. elegans reference genome (ce10) using HISAT2
Aligned reads were then counted via htseq-count in intersection (nonempty) mode without strandedness and default parameters
Normalized gene/transcript counts were then determined using DESeq2 with the local fit option and the outlier filtering and replacement options enabled
Genome_build: ce11
Supplementary_files_format_and_content: tab-delimited text files include hit counts for each individual sample; additionally there is a tab-delimited text file with all normalized counts

organism: Caenorhabditis elegans

source_name: whole worm

growth_protocol: L1 arrested C. elegans were grown on standard NGA media plates spotted with OP50 for 48 hours at 20 degrees Celsius

extract_protocol:
1000-2000 worms per sample were collected for RNA extraction. Extraction was performed by adding 1 mL QIAzol Lysis Reagent to the worm pellet and freezing. After two freeze/thaw cycles, total RNA was extracted and purified utilizing the QIAGEN miRNeasy Mini Kit according to manufacturer's protocol. Total RNA quality was checked with an Advanced Analytical Fragment Analyzer, and RQNs greater than 7 wer accepted.
Approximately 500 ng total RNA was used for library preparation by folloiwng the Illumina TruSeq Stranded mRNA Sample Preparation Guide.


title: F01D4.5(baf20) rep5


title: F01D4.5(baf20) rep2


title: dgriG1_m_vs_R4.leftover

description: mixed-species RNA preparation


title: F01D4.5(baf20) rep1


title: 257.01_D6_V

city: Stanford

first: Stephen

last: Popper

state: CA

department: Division of Infectious Diseases, Dept. of Medicine

institute: Stanford University

description: 257.01_D6_V paxgene total RNA amplified with targetamp, vs amplified Stratagene UHR, with 10ng v4 MJ doping control aRNA

hyb_protocol: reference URL : http://dx.doi.org/10.1371/journal.pntd.0001966 Hybridization method : 8 ug of Cy5-labeled amplified RNA and 5 ug of Cy3-labeled Universal Human Reference aRNA (Stratagene) were hybridized to Human Exonic Evidence Based Oligonucleotide (HEEBO) microarrays at 65C using the HYBEX hybridization system (SciGene) for 14 hours. Following hybridization, microarrays were washed in a series of four solutions containing 2_ SSC with 0.05% SDS, 2x SSC, 1x SSC, and 0.2x SSC, respectively. The first wash was performed for 5 min at 60C; subsequent washes were performed at room temperature for 2 min each.

scan_protocol: Scan Power : 100 PMT Volts : 519 Pixel Size : 10 Laser on time : 166865 Laser Power : 3.10 Scan Date : 2011-12-02 Scan Temperature : 27.52 Scan Time : 10:24:33 Scanner Make : Axon Instruments (V1.00) Scanner Model : GenePix 4000B [81971] Scanning software : AxImageIO Scanning software version : 2.0.3.2 Focus Position : 0

data_processing:
Extraction Software : SpotReader
Extraction Software Version : 1.0
Datafile type : GenePix Results 3
Image Origin : 640, 5400
Note: The order of lines in the supplemental raw files matches the order of lines in the Platform table and Sample table.

organism: Homo sapiens

source_name: Strat UHR and v4 MJ doping control aa-aRNA

extract_protocol: Extraction method : Human Universal Reference RNA (Stratagene)

tag: sample name

label_protocol:
Type of label : Cy5
reference URL : http://www.gelifesciences.com/webapp/wcs/stores/servlet/ProductDisplay?categoryId=11158&catalogId=10101&productId=16547&storeId=11787&langId=-1

extract_protocol:
Extraction method : PreAnalytiX PAXgene Blood RNA Kit
Amplification method : Epicentre TargetAmp Aminoallyl aRNA Amp Kit

treatment_protocol:
Treatment : Vaccine
Day : 6

tag: tissue type

value: Whole Bood


title: WT rep5


title: dgriG1_m_tx_R3.leftover

description: mixed-species RNA preparation


title: F01D4.5(baf20) rep3


title: dgriG1_m_vs_R1.leftover

description: mixed-species RNA preparation


title: WT rep4


title: WT rep3


title: WT rep2


title: dgriG1_m_re_R4.leftover

description: mixed-species RNA preparation


title: WT rep1


title: dgriG1_m_hd_R3.leftover

description: mixed-species RNA preparation


title: dgriG1_m_re_R2a.leftover

description: mixed-species RNA preparation


title: dgriG1_m_hd_R4.leftover

description: mixed-species RNA preparation


title: dgriG1_m_hd_R2.leftover

description: mixed-species RNA preparation


title: dgriG1_m_go_R4.leftover

description: mixed-species RNA preparation


title: dgriG1_m_tx_R2.leftover

description: mixed-species RNA preparation


title: dgriG1_m_go_R2.leftover

description: mixed-species RNA preparation


title: dgriG1_m_go_R3.leftover

description: mixed-species RNA preparation


title: dgriG1_m_ge_R4.leftover

description: mixed-species RNA preparation


title: dgriG1_m_ge_R3.leftover

description: mixed-species RNA preparation


title: dgriG1_m_ge_R2.leftover

description: mixed-species RNA preparation


title: dgriG1_m_ge_R1.leftover

description: mixed-species RNA preparation


title: Squamous cell carcinoma skin lesion sample 8A (BCC)

city: Louisville

first: J.

middle: Christopher

last: States

state: KY

address: 505 S. Hancock St., Rm 304

department: Department of Pharmacology & Toxicology

institute: University of Louisville

description: Arsenic-induced skin lesions

data_processing:
The data were collected at 0.1 threshold value on an Applied Biosystems ViiA7 Real Time PCR System. The Ct values were normalized to the mean of Ct values of U6 RNA (ΔCT method).
The ΔCt values were calculated using the Ct values of the miRNAs from premalignant or malignant lesions and normalizing them to the means of the Ct values of the reference RNA U6.
Fold changes were calcuated from the ΔΔCt values from the ΔCt values of malignant - premalignant lesions or malignant - malignant lesions

label: FAM

source_name: Human skin lesions from males exposed to levels of arsenic exceeding 50 ppb in drinking water located in Murshidibad district of West Bengal, India.

label_protocol: Profiling miRNA expression from isolated total RNA from skin lesions keratinocytes was performed using the TaqMan® Array Human MiRNA A Card v2.0 (polymerase chain reaction (RT-qPCR) array cards, Life Technologies).

extract_protocol: Keratinocytes were isolated from 7 μm sections of samples using an ArcturusXT™ Laser Capture Microdissection System. The sectioned tissues were first deparaffinized and stained using the Arcturus Paradise Plus staining kit and the isolated cells were collected on macrocaps. Total RNA was purified from the tissues on the macrocaps using Arcturus Paradise Plus FFPE RNA extraction and Isolation Kits. Total RNA quality and quantity were determined using the Agilent RNA 6000 Pico Kit, Eukaryote, version 2.6 and the Agilent 2100 Bioanalyzer instrument (Agilent Technologies, Inc., Santa Clara, CA, USA). Total RNA (50-100 ng) from each sample was reverse transcribed and pre-amplified to prepare cDNA using RT kit, MegaPlex Primers and Pre-Amp kit (Life Technologies) following the Megaplex™ Pools For microRNA Expression Analysis protocol with preamplification.

value: keratinocytes from FFPE lesions


title: Squamous cell carcinoma skin lesion sample 17A (BCC)

description: Arsenic-induced skin lesions


title: Basal cell carcinoma skin lesion sample 18B (SCC)

description: Arsenic-induced skin lesions


title: Squamous cell carcinoma skin lesion sample 20C (BCC)

description: Arsenic-induced skin lesions


title: dgriG1_m_re_R3.leftover

description: mixed-species RNA preparation


title: Basal cell carcinoma skin lesion sample 26A (SCC)

description: Arsenic-induced skin lesions


title: dgriG1_m_ac_R4.leftover

description: mixed-species RNA preparation


title: Hyperkeratosis skin lesion sample 19D (HK)

description: Arsenic-induced skin lesions


title: Basal cell carcinoma skin lesion sample 28B (SCC)

description: Arsenic-induced skin lesions


title: Hyperkeratosis skin lesion sample 28E (HK)

description: Arsenic-induced skin lesions


title: dgriG1_m_re_R1.leftover

description: mixed-species RNA preparation


title: Forsythia_suspensa_Leaf

city: Chiba

first: Mami

last: Yamazaki

address: Inohana 1-8-1, Chuo-ku

department: Graduate School of Pharmaceutical Sciences

country: Japan

institute: Chiba University

data_processing:
For de novo transcriptome assembly, we pre-processed raw reads, and removed adapter sequences, empty reads, reads with ambiguous ‘N’ bases > 5%, raw reads of low quality (Q<20), and raw reads with an average length less than 30 bases. Filtering of raw reads was performed using Trimmomatic program (Bolger et al., 2014), resulting into paired end raw reads, and unpaired reads which lost its corresponding sequence partner due to quality control.
The Trinity program ver 2.0.6 was used for the de novo transcriptome assembly using clean reads from all the samples. De novo assembly was further processed through CD-HIT-EST program (Li and Godzik 2006) to remove transcripts redundancies, resulting in our final assembly of 81,913 unigenes with N50 value as 1,307 bps.
We then processed these contigs for read alignment and abundance estimation using Bowtie2.0 and RSEM respectively. To calculate unigene expression, we used Fragments per Kilobase exon per Million mapped fragments (FPKM) method.
Supplementary_files_format_and_content: .fasta file was generated using CD-HIT-EST program. De novo transcriptome assembly of F. suspensa was constructed using the Trinity program ver 2.0.6, and processed through CD-HIT-EST program to remove transcripts redundancies. These unigene sequences generated were used for further analysis.
Supplementary_files_format_and_content: .results files were generated using RSEM program. Clean reads for each tissues were aligned on F. suspensa de novo transcriptome assembly (.fasta file), and abundace were measured using RSEM. Content of each file contains transcript_id (which is contig), gene_id (which is unigene), length, effective_length, expected_count, TPM, FPKM and IsoPct for each tissues. FPKM values were calculated using RSEM program. FPKM values for each tissues were used for further analysis.
Supplementary_files_format_and_content: Tab-delimited text file includes transcript_id, gene_id, seq_length, and FPKM values for each tissues. .results files for both tissues were used to extract FPKM values for each tissues, and were combined/aligned against transcript_id and gene_id.

organism: Forsythia suspensa

source_name: Plant Material in NIBIOHN

growth_protocol: Flower and leaf tissues were collected from F. suspensa growing in the natural conditions at the Tsukuba Division, Research Center for Medicinal Plant Resources, National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN, Tsukuba, Japan) in April, 2014.

extract_protocol:
Frozen samples of F. suspensa were powdered for RNA extraction. Total RNA for each of the two tissues was extracted using RNeasy Plant Mini Kit (Qiagen, USA), according to the manufacturer’s instruction.
cDNA library was then prepared using SureSelect Strand Specific RNA Library kit (Agilent 150 Technologies, USA) according to the manufacturer’s specifications. mRNA was isolated from total RNA of each tissue using beads containing oligo(dt), which was then added with fragmentation buffer to shear mRNA into short fragments and used as a template for the synthesis of first strand cDNA synthesis using random hexamer primers. The cDNA library for Illumina sequencing was prepared using SureSelect Strand Specific RNA Library kit (Agilent, USA) according to the manufacturer’s instruction.

treatment_protocol: Not applicable

value: Leaf

tag: age


title: dgriG1_m_hd_R1.leftover

description: mixed-species RNA preparation


title: Forsythia_suspensa_Flower

value: Flower


title: Hyperkeratosis skin lesion sample 14E (HK)

description: Arsenic-induced skin lesions


title: dgriG1_m_ac_R2.leftover

description: mixed-species RNA preparation


title: dgriG1_m_ac_R1.leftover

description: mixed-species RNA preparation


title: dgriG1_f_wb_R4.leftover

description: mixed-species RNA preparation


title: Succinate_2

city: Vancouver

first: David

last: Levy-Booth

state: British Columbia

address: 2350 Health Sciences Mall

department: Microbiology and Immunology

country: Canada

institute: University of British Columbia

description: Rhodococcus EP4 Succinate 2

data_processing:
Filtered to Q30 using Trimmomatic 0.3.6
Mapped to transcriptome with bowtie2
Quantified with Salmon
Normalized counts with deseq2
Genome_build: https://www.ncbi.nlm.nih.gov/genome/11941?genome_assembly_id=367165
Supplementary_files_format_and_content: Normalized deseq2 counts (.csv)

organism: Rhodococcus rhodochrous

source_name: Rhodococcus EP4 culture

growth_protocol: Rhodococcus EP4 for RNA extraction grown at 30°C grown to mid-log phase on M9-Goodies with 1.0 mM of each compound in 50ml Erlenmeyer flasks.

extract_protocol:
TRIzol RNA extraction
Ribosomal RNA depletion with Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) + TruSeq LT (Illumina) library preparation
1 lane (1/9 samples per lane) HiSeq4000 100bp-PE sequencing.

treatment_protocol: 1.0 mM each compound as carbon source

tag: growth-phase

value: mid-log

tag: compound

value: succinate

tag: media


title: dgriG1_m_tx_R4.leftover

description: mixed-species RNA preparation


title: dgriG1_f_wb_R3.leftover

description: mixed-species RNA preparation


title: dgriG1_m_go_R1.leftover

description: mixed-species RNA preparation


title: Succinate_1

description: Rhodococcus EP4 Succinate 1


title: Succinate_3

description: Rhodococcus EP4 Succinate 3


title: dgriG1_f_wb_R2.leftover

description: mixed-species RNA preparation


title: Propylguaiacol_2

description: Rhodococcus EP4 Propylguaiacol 2

value: propylguaiacol


title: dgriG1_f_wb_R1.leftover

description: mixed-species RNA preparation


title: dgriG1_f_vs_R4.leftover

description: mixed-species RNA preparation


title: Propylguaiacol_1

description: Rhodococcus EP4 Propylguaiacol 1


title: dgriG1_f_vs_R2.leftover

description: mixed-species RNA preparation


title: Ethylphenol_1

description: Rhodococcus EP4 Ethylphenol 1

value: ethylphenol


title: Ethylphenol_2

description: Rhodococcus EP4 Ethylphenol 2


title: Ethylphenol_3

description: Rhodococcus EP4 Ethylphenol 3


title: dgriG1_f_vs_R3.leftover

description: mixed-species RNA preparation


title: dgriG1_f_tx_R1.leftover

description: mixed-species RNA preparation


title: dgriG1_f_tx_R2.leftover

description: mixed-species RNA preparation


title: dgriG1_f_tx_R4.leftover

description: mixed-species RNA preparation


title: dgriG1_f_re_R4.leftover

description: mixed-species RNA preparation


title: dgriG1_f_tx_R3.leftover

description: mixed-species RNA preparation


title: dgriG1_m_ac_R3.leftover

description: mixed-species RNA preparation


title: dgriG1_f_re_R3.leftover

description: mixed-species RNA preparation


title: Propylguaiacol_3

description: Rhodococcus EP4 Propylguaiacol 3


title: dgriG1_f_re_R1.leftover

description: mixed-species RNA preparation


title: dgriG1_f_re_R2.leftover

description: mixed-species RNA preparation


title: dgriG1_f_hd_R4.leftover

description: mixed-species RNA preparation


title: dgriG1_f_hd_R3.leftover

description: mixed-species RNA preparation


title: dgriG1_f_vs_R1.leftover

description: mixed-species RNA preparation


title: dgriG1_f_hd_R1.leftover

description: mixed-species RNA preparation


title: dgriG1_f_go_R2.leftover

description: mixed-species RNA preparation


title: dgriG1_f_go_R4.leftover

description: mixed-species RNA preparation


title: dgriG1_f_go_R3.leftover

description: mixed-species RNA preparation


title: Slide US45102948_256525610006_RS01.2

city: STOCKHOLM

first: Sulman

last: Shafeeq

address: nobel vag 16

department: MTC

country: Sweden

institute: Karolinska Institutet

description: Sample 1

hyb_protocol: The protocol was performed as described in Kloosterman et al., 2006 and Shafeeq et al., 2011.

scan_protocol: Scanning was done using the Genepix 4200AL laser scanner.

data_processing: Dual-channel array images were analyzed with Genepix 6.  Spots were screened visually to identify those of low quality. Slide data were processed with MicroPreP software. Prior to analysis, automatically and manually flagged spots and spots with very low background subtracted signal intensity (5% of the weakest spots [sum of Cy3 and Cy5 net signals]) were filtered out of all data sets. Net signal intensities were calculated by grid-based background subtraction. A grid-based Lowess transformation was performed for slide normalization, negative and empty values were removed, and outliers were removed by the deviation test. Expression ratios  were calculated. Further analysis was performed with a Cyber-T Student T-test for paired data.

organism: Streptococcus pneumoniae D39

source_name: rex mutant

label_protocol: Total RNA was incubated for 16 h at 42°C in the presence of 400 U Superscript III RNase H reverse transcriptase (Invitrogen), 0.2 mM aminoallyl dUTP (Amersham), 0.5 mM dATP, 0.5 mM dCTP, 0.5 mM dGTP, 0.3 mM dTTP, and 3.2 g random nonamers. The synthesized cDNA was then labeled by coupling either Cy3 or Cy5 to the dUTP aminoallyl reactive group (CyScribe postlabeling kit; Amersham Biosciences) and was purified using a GFX PCR, DNA, and gel band purification kit (Amersham Biosciences).

growth_protocol: Cells were grown in CDM and harvested at at OD600 of 0.3.

extract_protocol: RNA was isolated and purified using the High Pure RNA isolation kit (Roche diagnostics) as described (Kloosterman et al., 2006). Contaminating genomic DNA was removed by treatment with RNase-free DNase I (Roche diagnostics). RNA was isolated from two replicate cultures.

tag: genotype/variation

value: rex mutant

source_name: wild-type

value: wild-type


title: dgriG1_f_hd_R2.leftover

description: mixed-species RNA preparation

value: not applicable


title: control female

city: Johnson City

first: Charles

middle: Albert

last: Stuart

state: TN

address: ETSU Quillen, PO 70622

department: Internal Medicine

institute: Quillen College of Medicine

description:
control female
A1

data_processing: copies per well normalized to the average of SNORD95, SNORD96A, and RNU6-2, subject A4 control

label: SYBR Green

source_name: vastus lateralis

label_protocol: Qiagen miScript

extract_protocol: Triazol

value: F

tag: disease state

value: control

tag: bmi

value: vastus lateralis


title: Slide US45102948_256525610001_RS01.3

description: Sample 1

growth_protocol: Cells were grown in CDM and harvested at respective mid-exponential phase. CDM was prepared without niacin.

value: wild type

tag: treated with

value: 0 mg/ml NADH

value: 0.5 mg/ml NADH


title: obese male

description:
obese male
A2

value: M

value: obese


title: Slide US45102948_256525610001_RS01.4

description: Sample 2


title: control male

description:
control male
A4

source_name: gastrocneneus

value: gastrocneneus


title: dgriG1_f_ge_R4.leftover

description: mixed-species RNA preparation

tag: strain


title: CuO-IRF7 H6N2 rep 1

city: Davis

first: Huaijun

last: Zhou

address: Meyer 3307, One shields Ave.

institute: UC Davis

data_processing:
Quality control; fastQC (version 0.11.6)
Read trim; TrimGalore (version 0.4.5)
Alignment; STAR (version 2.6.0)
Extract raw read counts from alignment; HTseq (version 0.9.0)
Genome_build: galGal5, NCBI annotation release 103
Supplementary_files_format_and_content: csv, raw read count from all the samples

organism: Gallus gallus

source_name: DF-1 cell line

growth_protocol: Immortalized chicken embryonic fibroblast DF-1 cells (ATCC, Manassas, VA, USA) were cultured in Dulbecco's modified Eagle's medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific), 1x Antibiotic-antimycotic (Thermo Fisher Scientific), and incubated at 37°C in a humidified atmosphere containing 5% CO2.

extract_protocol:
TRIzol was directly added to the cell monolayer and followed by Direct-zol coulmn based RNA extraction
polyA selected non-stranded cDNA library

tag: cell line

tag: genotype

value: IRF7 Overexpression

tag: infection


title: obese female

description:
obese female
A3

tag: tissue


title: dgriG1_f_go_R1.leftover

description: mixed-species RNA preparation

tag: Sex

tag: replicate


title: dgriG1_f_ge_R3.leftover

description: mixed-species RNA preparation


title: Slide US45102948_256525610006_RS01.5

description: Sample 2


title: Control H6N2 rep 2

value: Control


title: WT Mtb NHS 3

city: Bangalore

first: Genotypic

last: technology

state: Karnataka

address: 259, Apoorva 4th cross,80 feet Road,RMV 2ND STAGE

country: India

institute: Genotypic Technology

description: gene expression without heat-shock

hyb_protocol: 600ng of labeled cRNA sample were fragmented at 60°C and hybridized on to a Genotypic designed M. tb Gene Expression  8x15k Array (AMADID: 030010). Fragmentation of labeled cRNA and hybridization were done using the Gene Expression Hybridization kit (Agilent Technologies, In situ Hybridization kit, Part Number 5190-0404). Hybridization was carried out in Agilent’s Surehyb Chambers at 65° C for 16 hours.

scan_protocol: Agilent Microarray Scanner (Agilent Technologies, Part Number G2600D).

data_processing: Images were quantified using Feature Extraction Software ( Agilent). Feature extracted raw data was analyzed using GeneSpring GX software from Agilent. Normalization of the data was done in GeneSpring GX using the percentile shift method normalization method .Gene based analysis was performed.

organism: Mycobacterium tuberculosis

label_protocol: The samples for Gene expression were labeled using Agilent Quick-Amp labeling Kit (p/n5190-0442). 500ng each of total RNA were reverse transcribed at 40°C using oligo dT primer tagged to a T7 polymerase promoter and converted to double stranded cDNA. Synthesized double stranded cDNA were used as template for cRNA generation. cRNA was generated by in vitro transcription and the dye Cy3 CTP(Agilent) was incorporated during this step. The cDNA synthesis and in vitro transcription steps were carried out at 40°C. Labeled cRNA was cleaned up using Qiagen RNeasy columns (Qiagen, Cat No: 74106) and quality assessed for yields and specific activity using the Nanodrop ND-1000.

growth_protocol: cells grown in 7H9 medium at 37ºC till mid-log phase

extract_protocol: RNA extraction was perforemd by using Qiagen Rneasy mini kit with Dnase Treatment.

treatment_protocol: Mid-log phase cultures of Mtb were given heat-shock at 45ºC for 90 mins

value: Wild type


title: phoP mutant HS 3

description: gene expression after 1hr30min heat-shock

source_name: Issar smith and co-workers

value: phoP mutant


title: phoP mutant NHS 1

description: gene expression without heat-shock


title: phoP mutant NHS 2

description: gene expression without heat-shock


title: dgriG1_f_ge_R2.leftover

description: mixed-species RNA preparation


title: Control H6N2 rep 1


title: WT Mtb HS 3


title: WT Mtb NHS 2

description: gene expression without heat-shock


title: WT Mtb NHS 1

description: gene expression without heat-shock


title: CuO-IRF7 H6N2 rep 2


title: dgriG1_f_ge_R1.leftover

description: mixed-species RNA preparation


title: phoP mutant NHS 3

description: gene expression without heat-shock


title: dgriG1_f_ac_R4.leftover

description: mixed-species RNA preparation


title: CuO-IRF7 Mock rep 2

value: Mock


title: CuO-IRF7 Mock rep 1


title: dgriG1_f_ac_R3.leftover

description: mixed-species RNA preparation


title: phoP mutant HS 2


title: dgriG1_f_ac_R2.leftover

description: mixed-species RNA preparation


title: phoP mutant HS 1


title: WT Mtb HS 2


title: Control Mock rep 2


title: Control Mock rep 1


title: dgriG1_f_ac_R1.leftover

description: mixed-species RNA preparation


title: dgriG1_m_tx_R1.leftover

description: mixed-species RNA preparation


title: Healthy control 12

status: Public on May 31 2018

city: La Jolla

first: Nicholas

last: Wu

address: Beckman Build, BCC206, 10650 North Torrey Pines Road

institute: The Scripps Research Institute

description: Gene expression data from Healthy control 12

hyb_protocol: Hybridization was performed according to the standard protocol (GeneChip® Expression Wash, Stain and Scan User Manual).

scan_protocol: Arrays were scanned by Affymetrix GeneChip Scanner 3000 (Cat#00-00213, Affymetrix, Santa Clara, CA, US). Command Console Software (Affymetrix, Santa Clara, CA, US) was used to control the scanner and summarize probe cell intensity data (CEL file generation) with default settings.

data_processing: The raw microarray data were normalized using Robust Multi-array Average (RMA).

source_name: Peripheral blood

label_protocol: Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).

extract_protocol: For each sample, RNA was extracted using Tempus™ Spin RNA Isolation Kit (Applied Biosystems) from 3 mL of peripheral blood according to manufacturer’s instructions.

tag: subject category

tag: number of day post-infection


title: Healthy control 14

description: Gene expression data from Healthy control 14


title: Healthy control 5

description: Gene expression data from Healthy control 5


title: Patient 9, day 15 post-infection

description: Gene expression data from Patient 9, day 15 post-infection

value: Patient


title: Patient 9, day 18 post-infection

description: Gene expression data from Patient 9, day 18 post-infection


title: WT Mtb HS 1


title: Healthy control 6

description: Gene expression data from Healthy control 6


title: Healthy control 15

description: Gene expression data from Healthy control 15


title: Healthy control 13

description: Gene expression data from Healthy control 13


title: Patient 10, day 9 post-infection

description: Gene expression data from Patient 10, day 9 post-infection


title: Healthy control 10

description: Gene expression data from Healthy control 10


title: Patient 6, day 26 post-infection

description: Gene expression data from Patient 6, day 26 post-infection


title: Healthy control 7

description: Gene expression data from Healthy control 7


title: Patient 7, day 22 post-infection

description: Gene expression data from Patient 7, day 22 post-infection


title: Patient 6, day 8 post-infection

description: Gene expression data from Patient 6, day 8 post-infection


title: Patient 4, day 23 post-infection

description: Gene expression data from Patient 4, day 23 post-infection


title: Patient 8, day 19 post-infection

description: Gene expression data from Patient 8, day 19 post-infection


title: Patient 7, day 8 post-infection

description: Gene expression data from Patient 7, day 8 post-infection


title: Patient 6, day 17 post-infection

description: Gene expression data from Patient 6, day 17 post-infection


title: Healthy control 11

description: Gene expression data from Healthy control 11


title: Healthy control 9

description: Gene expression data from Healthy control 9


title: Healthy control 4

description: Gene expression data from Healthy control 4


title: Healthy control 8

description: Gene expression data from Healthy control 8


title: Patient 7, day 16 post-infection

description: Gene expression data from Patient 7, day 16 post-infection


title: Patient 8, day 29 post-infection

description: Gene expression data from Patient 8, day 29 post-infection


title: Patient 10, day 16 post-infection

description: Gene expression data from Patient 10, day 16 post-infection


title: Patient 9, day 10 post-infection

description: Gene expression data from Patient 9, day 10 post-infection


title: Patient 8, day 12 post-infection

description: Gene expression data from Patient 8, day 12 post-infection


title: 5045-1-S

city: NEDLANDS

last: Martino

state: Western Australia

address: Perth Childrens Hospital, 15 Hospital Avenue

country: Australia

institute: Telethon Kids Institute

data_processing:
Fastq files were assessed for quality using the FastQC package and no samples were excluded from analysis.
The Salmon aligner (v0.8.2, 50) was used to map 75-bp paired end reads to the human transcriptome (Ensembl, GRCh37v75).
Uniquely mapped reads were summarized across all genes with the tximport program
Genes not highly expressed were filtered out (less than 10 counts per million)
The counts data was converted to DGEList object and TMM normalized to remove library composition bias using the limma package
Genome_build: GRCh37v75
Supplementary_files_format_and_content: .csv flat files contain TMM normalized counts

growth_protocol: Ex-vivo viable naïve CD4+ T-cells were seeded in 96-well round bottom plates at a density of 8 x 1e^4 cells per well in a total volume of 200µL of RPMI media supplemented with 10% FCS, 100U/mL penicillin-streptomycin, 2mM L-glutamine + rhIL2 (200U/mL, Life technologies) for 72 hours.

extract_protocol:
At termination of cultures, activated and resting cells were recovered and centrifuged at 500 x g for 5 minutes in RPMI media and cell pellets lysed in 350µL of RLT buffer from the Qiagen AllPrep DNA/RNA extraction micro kit (Qiagen). Genomic DNA and total RNA were extracted according to manufacturer’s instructions using the QIACube automated liquid handling robot (Qiagen).
Library preparation was performed using the Illumina TruSeq stranded mRNA kit with a starting input of 50ng and libraries were sequenced on the Illumina HiSeq 4000 instrument generating an average of 20 million reads per sample.

treatment_protocol: T-cells were divided in half and either activated with 2µ of Human T-cell activator CD3/CD28 Dynabeads (Thermo Fisher Scientific) per well (1:1 ratio bead-to-cell) or left resting in media alone for 72 hours at 37°C and 5% CO2

tag: indiidual

value: male

value: caucasian

value: allergic


title: 5045-2-S

value: resolved


title: 5045-2-U


title: Patient 10, day 20 post-infection

description: Gene expression data from Patient 10, day 20 post-infection


title: 5045-1-U


title: 5037-2-U

value: female


title: 5037-1-U


title: 4938-2-S


title: 5037-2-S


title: 4938-1-U


title: 5037-1-S


title: 4938-1-S


title: 4834-2-U


title: 4938-2-U


title: Patient 4, day 17 post-infection

description: Gene expression data from Patient 4, day 17 post-infection


title: 4834-2-S


title: Patient 2, day 22 post-infection

description: Gene expression data from Patient 2, day 22 post-infection


title: Patient 3, day 17 post-infection

description: Gene expression data from Patient 3, day 17 post-infection


title: Patient 3, day 23 post-infection

description: Gene expression data from Patient 3, day 23 post-infection


title: Patient 3, day 21 post-infection

description: Gene expression data from Patient 3, day 21 post-infection


title: 4834-1-U


title: Patient 3, day 28 post-infection

description: Gene expression data from Patient 3, day 28 post-infection


title: Healthy control 1

description: Gene expression data from Healthy control 1


title: Healthy control 2

description: Gene expression data from Healthy control 2


title: Healthy control 3

description: Gene expression data from Healthy control 3


title: 4792-2-U


title: 4834-1-S


title: 4792-1-U


title: 4792-1-S


title: Patient 2, day 13 post-infection

description: Gene expression data from Patient 2, day 13 post-infection


title: Patient 2, day 11 post-infection

description: Gene expression data from Patient 2, day 11 post-infection


title: Patient 2, day 15 post-infection

description: Gene expression data from Patient 2, day 15 post-infection


title: Patient 3, day 15 post-infection

description: Gene expression data from Patient 3, day 15 post-infection


title: Patient 4, day 14 post-infection

description: Gene expression data from Patient 4, day 14 post-infection


title: 4523-2-U

value: other


title: 4792-2-S


title: 4523-2-S


title: 4260-2-U

value: asian


title: 4523-1-S


title: 4260-1-S


title: 4086-2-U


title: 4260-2-S


title: 4086-2-S


title: 4260-1-U


title: 4086-1-S


title: 3924-1-S


title: 3924-1-U


title: 3924-2-S


title: 3823-2-U


title: 4523-1-U


title: 3823-1-U


title: 3823-1-S


title: 3817-1-U


title: 3817-2-S


title: 3817-1-S


title: 3817-2-U


title: 3578-1-U


title: 3808-1-U


title: 3578-1-S


title: 3578-2-U


title: 3537-2-S


title: 3808-2-S


title: 3578-2-S


title: 3808-2-U


title: 3808-1-S


title: 3537-1-S


title: 3537-1-U


title: 3364-2-U


title: 3325-2-U


title: 3364-2-S


title: 3823-2-S


title: 3325-1-U


title: 3364-1-U


title: 3325-2-S


title: 3325-1-S


title: 3316-2-U

value: mixed


title: 3316-2-S


title: 3316-1-U


title: 3316-1-S


title: 3171-2-U


title: 3053-2-S


title: 3053-2-U


title: 3171-1-U


title: 3171-1-S


title: 3171-2-S


title: 3053-1-S


title: 3053-1-U


title: 2898-1-U


title: 3364-1-S


title: 2874-1-U


title: 2963-1-U

value: control


title: 2898-2-S


title: 2874-2-S


title: 2994-2-S


title: 2874-1-S


title: 2898-2-U


title: 2963-1-S


title: 2859-1-U


title: 2994-1-S


title: 2994-1-U


title: 2859-1-S


title: 2832-1-S


title: 2832-1-U


title: 2898-1-S


title: 2859-2-S


title: 2789-1-U


title: 2789-1-S


title: 2994-2-U


title: 2748-2-S


title: 2748-1-S


title: 2748-1-U


title: 2683-1-U


title: 2628-1-U


title: 2683-1-S


title: 2874-2-U


title: 2859-2-U


title: 2628-1-S


title: 2601-1-U


title: 2601-1-S


title: 2597-1-S


title: 2561-1-S


title: 2549-1-U


title: 2476-1-S


title: 2476-1-U


title: 2448-1-U


title: 2448-1-S


title: 2414-1-U


title: 2561-1-U


title: 2414-2-U


title: 2414-2-S


title: 2549-1-S


title: 2329-1-U


title: 2414-1-S


title: 2349-1-S


title: 2748-2-U


title: 2286-1-S


title: 2286-1-U


title: 2262-1-S


title: 2329-1-S


title: 2597-1-U


title: 2262-1-U


title: 2349-1-U


title: 2025-2-S


title: 2070-1-U


title: 2025-1-S


title: 2008-1-U


title: 2070-1-S


title: 2025-1-U


title: 2008-1-S


title: 2025-2-U


title: 22RV1_ATAC_seq rep1

city: Durham

first: Zhong

last: Chen

state: NC

address: 905 S. LaSalle Street

department: Pathology

institute: Duke University

description: Biological replicate 1

library_source: genomic

data_processing:
ATAC-seq Alignment: Sequence reads were obtained and mapped to the human (hg19) genomes using the Bowtie2 with standard parameters and a maximum fragment length of 2,000. Picard was then used to remove duplicate reads.
Genome_build: hg19
Supplementary_files_format_and_content: For ATAC-seq, bedGraph files were generated using bedtools, scores represent peaks genomic coverage.

molecule: genomic DNA

source_name: prostate cancer cells

growth_protocol: The androgen-independent prostate cancer cell line 22RV1 was cultured in the RPMI complete medium (10% FBS).The androgen-independent prostate cancer cell line LN95 was cultured in the RPMI complete medium (10% CSFBS).

extract_protocol:
Cells were treated with DNase at a final concentration of 50 U/ml for 30 min. Then cells were washed, trypsinized and resuspended in cold PBS.
50,000 cells were resuspended in cold ATAC-seq resuspension buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, and 3 mM MgCl2). Cell nuclei were then prepared by incubation in 50 μl of ATAC-seq resuspendsion buffer containing 0.1% NP40, 0.1% Tween-20, and 0.01% digitonin on ice for 3 min. After centrifugation, nuclei were resuspended in 50 μl of transposition mix (25 μl 2× TD buffer ,2.5 μl Nextera Tn5 transposase (Illuminar), 16.5 μl PBS, 0.5 μl 1% digitonin, 0.5 μl 10% Tween-20, and 5 μl water), and incubated at 37 °C for 30 min in a thermomixer with shaking at 1,000 rpm. Transposed fragments were then purified with a Zymo DNA Clean and Concentrator-5 Kit. All libraries showed sufficient amplification after the 5 pre-amplification cycles and quantified using the KAPA Library Quantification Kit.

treatment_protocol: For all experiments, cells were maintained in phenol red-free RPMI medium with 5-10% charcoal-stripped FBS for 3 days before collection.

tag: cancer

value: Prostate adenocarcinoma

tag: chip andtibody


title: LN95_ATAC_seq rep2

description: Biological replicate 2


title: LN95_ATAC_seq rep1

description: Biological replicate 1


title: 22RV1_ATAC_seq rep2

description: Biological replicate 2


title: 2250-1-U


title: 2250-1-S


title: human neuroblastoma cell line SHSY5Y, replicate 2

city: Kansas City

first: Madelaine

last: Gogol

state: MO

address: 1000 E. 50th Street

department: Computational Biology Core

institute: Stowers Institute

data_processing:
Following sequencing, Illumina Primary Analysis version RTA 1.18.64 and bcl2fastq2 were run to demultiplex reads for all libraries and generate FASTQ files. FASTQ files were mapped to the human genome hg38 from UCSC using Tophat (2.1.1) with options –segment-mismatches 1 -x 1 -g 1. Ensembl 87 annotations were used to define gene coordinates. Quality of the samples was assessed using FastQC (0.10.1). The R (3.3.1) environment was employed for the statistical analysis of the data. FPKM values were generated with cufflinks (2.2.1) with default parameters.
Genome_build: human genome hg38 from UCSC
Supplementary_files_format_and_content: cufflinks (2.2.1) with default parameters generated FPKM values.

source_name: neuroblastoma cell line

growth_protocol: The human SHSY5Y neuroblastoma cell line was obtained from ATCC (CRL-2266) and maintained in 1:1 mixture of Eagles’s Minimum Essential Medium (ATCC, 30-2003) supplemented with 10% FBS (VWR, 97068-085). All cultures were determined to be free of mycoplasma contamination using a polymerase chain reaction-based detection system (Roche, 0518424001).

extract_protocol:
Total RNA was isolated from three, separate T25 flasks of adherent SHSY5Y cells at approximately 60% confluency using Sigma’s GenElute mammalian Total RNA miniprep kit (Millipore Sigma, RTN70) per the manufacturer’s recommendations. Total RNA was quantified on an Agilent Bioanalyzer 2100 using a Eukaryote Total RNA Nano chip (Agilent, 5067-1511) as well as a Nanodrop spectrophotometer (ThermoFisher Scientific, ND-1000).
Libraries were made from 500ng of total RNA according to the manufacturer’s directions for the TruSeq Stranded mRNA LT– set A and B (Illumina, Cat. No. RS-122-2101 and RS-122-2102) kit. Resulting short fragment libraries were checked for quality and quantity using the Bioanalyzer High Sensitivity DNA assay  (Agilent; 5067-4626) and Qubit Fluorometer (Life Technologies).

value: neuroblastoma cell line SHSY5Y


title: human neuroblastoma cell line SHSY5Y, replicate 3


title: human neuroblastoma cell line SHSY5Y, replicate 1


title: 4995.1.U

type: genomic

hyb_protocol: bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human MethylationEPIC Beadchip using standard Illumina protocol

scan_protocol: Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting

data_processing: update

label: Cy5 and Cy3

label_protocol: Standard Illumina Protocol

extract_protocol: At termination of cultures, activated and resting cells were recovered and centrifuged at 500 x g for 5 minutes in RPMI media and cell pellets lysed in 350µL of RLT buffer from the Qiagen AllPrep DNA/RNA extraction micro kit (Qiagen). Genomic DNA and total RNA were extracted according to manufacturer’s instructions using the QIACube automated liquid handling robot (Qiagen).

value: transient

tag: age

tag: allergy status

tag: stimulation


title: 5045.1.U


title: 5045.2.U


title: 4995.2.U


title: 5045.1.S


title: 5037.2.S


title: 5037.1.U


title: 4952.1.S

value: persistent


title: 5037.2.U


title: 4834.2.U


title: 4952.2.S


title: 4792.1.S


title: 4792.2.S


title: 4952.1.U


title: 5045.2.S


title: 4938.2.U


title: 4995.2.S


title: 4523.1.U


title: 5037.1.S


title: 4523.2.U


title: 4938.2.S


title: 4523.2.S


title: 4834.1.U


title: 4834.2.S


title: 4938.1.U


title: 4834.1.S


title: 4792.1.U


title: 4938.1.S


title: 4995.1.S


title: 4276.2.S


title: 4792.2.U


title: 4276.1.S


title: 4276.1.U


title: 4276.2.U


title: 4260.2.U


title: 4121.2.U


title: 4260.2.S


title: 4086.1.S


title: 3924.2.U


title: 3849.1.S


title: 4086.2.U


title: 3924.1.U


title: 3924.2.S


title: 4121.1.U


title: 3823.2.U


title: 3924.1.S


title: 4121.2.S


title: 3849.1.U


title: 3823.1.S


title: 3849.2.U


title: 3817.2.U


title: 3817.1.U


title: 3823.1.U


title: 3808.2.S


title: 3808.1.U


title: 4121.1.S


title: 3808.1.S


title: 3808.2.U


title: 3625.1.S


title: 3625.2.U


title: 3817.2.S


title: 3625.2.S


title: 3817.1.S


title: 3625.1.U


title: 3578.1.U


title: 3578.2.S


title: 3823.2.S


title: 3578.2.U


title: 3537.2.S


title: 3537.1.S


title: 3849.2.S


title: 3445.2.U


title: 3445.1.S


title: 3537.2.U


title: 3578.1.S


title: 3445.1.U


title: 3364.1.U


title: 3364.2.U


title: 4086.2.S


title: 4086.1.U


title: 3325.2.U


title: 3325.2.S


title: 3364.1.S


title: 4523.1.S


title: 3364.2.S


title: 3325.1.U


title: 3316.2.S


title: 3325.1.S


title: 3316.2.U


title: 3290.1.S


title: 3316.1.S


title: 3537.1.U


title: 3222.2.S


title: 3316.1.U


title: 3222.2.U


title: 3290.2.U


title: 3171.1.U


title: 3290.2.S


title: 3129.2.U


title: 3171.1.S


title: 3445.2.S


title: 3129.1.U


title: 3053.2.U


title: 3171.2.S


title: 3053.1.U


title: 3129.2.S


title: 3053.1.S


title: 2994.1.U


title: 3040.2.U


title: 3053.2.S


title: 2898.1.U


title: 2874.2.U


title: 2963.1.S

value: nonallergic


title: 3129.1.S


title: 2994.1.S


title: 2994.2.S


title: 3040.2.S


title: 2874.1.S


title: 2859.2.U


title: 2874.1.U


title: 2963.1.U


title: 2859.2.S


title: 2898.1.S


title: 2832.1.U


title: 2898.2.S


title: 3290.1.U


title: 2789.1.S


title: 2898.2.U


title: 2832.1.S


title: 2859.1.U


title: 3171.2.U


title: 2994.2.U


title: 2748.2.S


title: 2789.1.U


title: 2874.2.S


title: 2683.1.S


title: 2748.2.U


title: 2683.1.U


title: 2680.2.U


title: 2859.1.S


title: 2748.1.U


title: 2835.2.S


title: 2680.2.S


title: 2628.1.U


title: 2835.2.U


title: 2628.1.S


title: 2678.1.S


title: 2680.1.S


title: 2678.1.U


title: 2601.1.S


title: 2601.1.U


title: 2748.1.S


title: 2476.1.U


title: 2561.1.U


title: 2680.1.U


title: 2561.1.S


title: 2597.1.S


title: 2476.1.S


title: 2597.1.U


title: 2549.1.U


title: 2549.1.S


title: 2451.1.S


title: 2451.2.U


title: 2451.1.U


title: 2451.2.S


title: 2414.2.U


title: 2448.1.U


title: 2448.1.S


title: 2349.1.U


title: 2250.1.U


title: 2414.1.S


title: 2349.1.S


title: 2414.1.U


title: 2286.1.U


title: 2262.1.U


title: 2250.1.S


title: 2414.2.S


title: 2329.1.S


title: 2286.1.S


title: 2070.1.U


title: 2211.1.U


title: 2132.1.S


title: 2329.1.U


title: VATTRTg_rep1a1640

status: Public on May 26 2018

city: Boston

first: CBDM

last: Lab

state: MA

address: 77 Avenue Louis Pasteur

department: Microbiology and Immunobiology

institute: Harvard Medical School

data_processing:
fastq quality read filtering (>80% with Sanger Q>20)
cell barcode demultiplexing (custom script)
mapping with tophat2 on the mm10 transcriptome
filtering out low alignment score reads and multiple mapping reads (samtools)
duplication filtering using unique molecular identifiers (custom scripts). UMI sequence is at the 4rth position in the read ID (separation by ":")
Genome_build: mm10
Supplementary_files_format_and_content: raw read counts

source_name: VAT (epididymal)

growth_protocol: Live cells were sorted by flow cytometry from the spleen and visceral adipose tissue (epididymal fat) of mice (carrying a Foxp3 reporter gene).

extract_protocol:
Spleen were mechanically dissociated and visceral adipose tissue were digested with collagenase/dispase to single cell suspensions. Tregs cells were sorted by flow cytometry as DAPI- TCRb+ CD4+ GFP+ cells. Transgenic cells were sorted as TRAV2+ TRBV4+ cells. Single cells were then encapsulated in nanodroplets according to the InDrops protocol.
mRNA was reverse transcribed using adapters containing oligo dT in each droplet. The emulsion was then broken and linear amplification was performed by in vitro transcription. Illumina adapters were added by reverse transcription and PCR. Library were sequenced using a NextSeq500.

tag: library name

tag: sample type

value: B6 Foxp3-Ires-Gfp vTreg53 TCR-tg

tag: age (months)

value: Tg

value: VAT (epididymal)

tag: total number of reads

tag: number of mapped reads

tag: number of unique reads (umi)


title: VATTRTg_rep1a1638

tag: strain

value: male

tag: tissue


title: VATTRTg_rep1a1635

tag: Sex


title: 2211.1.S


title: 2262.1.S


title: VATTRTg_rep1a1645


title: VATTRTg_rep1a1634


title: VATTRTg_rep1a1643


title: VATTRTg_rep1a1642


title: VATTRTg_rep1a1630


title: VATTRTg_rep1a1631


title: 2025.1.U


title: 2009.1.U


title: 2070.1.S


title: VATTRTg_rep1a1627


title: VATTRTg_rep1a1629


title: 2025.2.U


title: VATTRTg_rep1a1626


title: 2025.2.S


title: VATTRTg_rep1a1624


title: 2009.1.S


title: VATTRTg_rep1a1623


title: VATTRTg_rep1a1628


title: VATTRTg_rep1a1637


title: VATTRTg_rep1a1620


title: 1923.2.U


title: VATTRTg_rep1a1617


title: 2009.2.U


title: 2009.2.S


title: 2025.1.S


title: VATTRTg_rep1a1614


title: VATTRTg_rep1a1616


title: VATTRTg_rep1a1609


title: VATTRTg_rep1a1618


title: VATTRTg_rep1a1612


title: VATTRTg_rep1a1611


title: VATTRTg_rep1a1606


title: VATTRTg_rep1a1619


title: VATTRTg_rep1a1604


title: VATTRTg_rep1a1603


title: VATTRTg_rep1a1607


title: VATTRTg_rep1a1608


title: 1923.2.S


title: VATTRTg_rep1a1600


title: VATTRTg_rep1a1597


title: VATTRTg_rep1a1599


title: VATTRTg_rep1a1601


title: VATTRTg_rep1a1598


title: 2008.1.S


title: VATTRTg_rep1a1595


title: VATTRTg_rep1a1594


title: VATTRTg_rep1a1591


title: VATTRTg_rep1a1596


title: VATTRTg_rep1a1593


title: VATTRTg_rep1a1589


title: VATTRTg_rep1a1588


title: VATTRTg_rep1a1590


title: 1391.2.U


title: VATTRTg_rep1a1587


title: VATTRTg_rep1a1583


title: 2008.1.U


title: VATTRTg_rep1a1579


title: VATTRTg_rep1a1581


title: VATTRTg_rep1a1575


title: VATTRTg_rep1a1574


title: 1399.2.U


title: VATTRTg_rep1a1570


title: VATTRTg_rep1a1577


title: VATTRTg_rep1a1572


title: VATTRTg_rep1a1582


title: VATTRTg_rep1a1565


title: VATTRTg_rep1a1566


title: VATTRTg_rep1a1562


title: VATTRTg_rep1a1602


title: VATTRTg_rep1a1580


title: 1391.1.S


title: VATTRTg_rep1a1556


title: VATTRTg_rep1a1559


title: VATTRTg_rep1a1568


title: VATTRTg_rep1a1571


title: VATTRTg_rep1a1553


title: VATTRTg_rep1a1569


title: 1391.2.S


title: VATTRTg_rep1a1557


title: 1923.1.U


title: VATTRTg_rep1a1555


title: 1399.2.S


title: VATTRTg_rep1a1551


title: VATTRTg_rep1a1550


title: VATTRTg_rep1a1585


title: VATTRTg_rep1a1548


title: 1391.1.U


title: VATTRTg_rep1a1560


title: VATTRTg_rep1a1554


title: VATTRTg_rep1a1546


title: VATTRTg_rep1a1541


title: VATTRTg_rep1a1549


title: VATTRTg_rep1a1539


title: VATTRTg_rep1a1537


title: VATTRTg_rep1a1545


title: VATTRTg_rep1a1547


title: VATTRTg_rep1a1536


title: VATTRTg_rep1a1534


title: VATTRTg_rep1a1532


title: VATTRTg_rep1a1530


title: VATTRTg_rep1a1538


title: VATTRTg_rep1a1544


title: VATTRTg_rep1a1523


title: VATTRTg_rep1a1524


title: VATTRTg_rep1a1522


title: VATTRTg_rep1a1527


title: VATTRTg_rep1a1533


title: VATTRTg_rep1a1540


title: VATTRTg_rep1a1528


title: VATTRTg_rep1a1531


title: VATTRTg_rep1a1525


title: VATTRTg_rep1a1526


title: VATTRTg_rep1a1515


title: VATTRTg_rep1a1514


title: VATTRTg_rep1a1517


title: VATTRTg_rep1a1513


title: VATTRTg_rep1a1520


title: VATTRTg_rep1a1529


title: VATTRTg_rep1a1501


title: VATTRTg_rep1a1499


title: VATTRTg_rep1a1519


title: VATTRTg_rep1a1496


title: VATTRTg_rep1a1497


title: VATTRTg_rep1a1509


title: VATTRTg_rep1a1510


title: VATTRTg_rep1a1506


title: VATTRTg_rep1a1494


title: VATTRTg_rep1a1567


title: VATTRTg_rep1a1503


title: VATTRTg_rep1a1486


title: VATTRTg_rep1a1485


title: VATTRTg_rep1a1492


title: VATTRTg_rep1a1490


title: VATTRTg_rep1a1484


title: VATTRTg_rep1a1495


title: VATTRTg_rep1a1476


title: VATTRTg_rep1a1493


title: VATTRTg_rep1a1483


title: VATTRTg_rep1a1472


title: VATTRTg_rep1a1481


title: VATTRTg_rep1a1482


title: VATTRTg_rep1a1498


title: VATTRTg_rep1a1477


title: VATTRTg_rep1a1488


title: VATTRTg_rep1a1474


title: VATTRTg_rep1a1508


title: VATTRTg_rep1a1470


title: VATTRTg_rep1a1464


title: VATTRTg_rep1a1491


title: VATTRTg_rep1a1473


title: VATTRTg_rep1a1471


title: VATTRTg_rep1a1456


title: VATTRTg_rep1a1458


title: VATTRTg_rep1a1469


title: VATTRTg_rep1a1463


title: VATTRTg_rep1a1465


title: VATTRTg_rep1a1452


title: VATTRTg_rep1a1468


title: VATTRTg_rep1a1462


title: VATTRTg_rep1a1475


title: VATTRTg_rep1a1467


title: VATTRTg_rep1a1447


title: VATTRTg_rep1a1445


title: VATTRTg_rep1a1457


title: VATTRTg_rep1a1459


title: VATTRTg_rep1a1460


title: VATTRTg_rep1a1454


title: VATTRTg_rep1a1446


title: VATTRTg_rep1a1437


title: VATTRTg_rep1a1435


title: VATTRTg_rep1a1439


title: VATTRTg_rep1a1444


title: VATTRTg_rep1a1443


title: VATTRTg_rep1a1430


title: VATTRTg_rep1a1431


title: VATTRTg_rep1a1451


title: VATTRTg_rep1a1434


title: VATTRTg_rep1a1422


title: VATTRTg_rep1a1419


title: VATTRTg_rep1a1427


title: VATTRTg_rep1a1428


title: VATTRTg_rep1a1418


title: VATTRTg_rep1a1438


title: VATTRTg_rep1a1436


title: VATTRTg_rep1a1412


title: VATTRTg_rep1a1410


title: VATTRTg_rep1a1409


title: VATTRTg_rep1a1429


title: VATTRTg_rep1a1426


title: VATTRTg_rep1a1425


title: VATTRTg_rep1a1408


title: VATTRTg_rep1a1432


title: VATTRTg_rep1a1420


title: VATTRTg_rep1a1417


title: VATTRTg_rep1a1414


title: VATTRTg_rep1a1415


title: VATTRTg_rep1a1402


title: VATTRTg_rep1a1407


title: VATTRTg_rep1a1404


title: VATTRTg_rep1a1411


title: VATTRTg_rep1a1413


title: VATTRTg_rep1a1401


title: VATTRTg_rep1a1400


title: VATTRTg_rep1a1403


title: VATTRTg_rep1a1393


title: VATTRTg_rep1a1416


title: VATTRTg_rep1a1421


title: VATTRTg_rep1a1398


title: VATTRTg_rep1a1386


title: VATTRTg_rep1a1387


title: VATTRTg_rep1a1385


title: VATTRTg_rep1a1382


title: VATTRTg_rep1a1381


title: VATTRTg_rep1a1391


title: VATTRTg_rep1a1392


title: VATTRTg_rep1a1396


title: VATTRTg_rep1a1394


title: VATTRTg_rep1a1379


title: VATTRTg_rep1a1375


title: VATTRTg_rep1a1388


title: VATTRTg_rep1a1389


title: VATTRTg_rep1a1373


title: VATTRTg_rep1a1368


title: VATTRTg_rep1a1383


title: VATTRTg_rep1a1372


title: VATTRTg_rep1a1364


title: VATTRTg_rep1a1380


title: VATTRTg_rep1a1376


title: VATTRTg_rep1a1367


title: VATTRTg_rep1a1365


title: VATTRTg_rep1a1395


title: VATTRTg_rep1a1361


title: VATTRTg_rep1a1357


title: VATTRTg_rep1a1363


title: VATTRTg_rep1a1356


title: VATTRTg_rep1a1355


title: VATTRTg_rep1a1362


title: VATTRTg_rep1a1359


title: VATTRTg_rep1a1340


title: VATTRTg_rep1a1343


title: VATTRTg_rep1a1335


title: VATTRTg_rep1a1347


title: VATTRTg_rep1a1346


title: VATTRTg_rep1a1337


title: VATTRTg_rep1a1350


title: VATTRTg_rep1a1352


title: VATTRTg_rep1a1349


title: VATTRTg_rep1a1378


title: VATTRTg_rep1a1341


title: VATTRTg_rep1a1345


title: VATTRTg_rep1a1325


title: VATTRTg_rep1a1351


title: VATTRTg_rep1a1333


title: VATTRTg_rep1a1323


title: VATTRTg_rep1a1322


title: VATTRTg_rep1a1321


title: VATTRTg_rep1a1348


title: VATTRTg_rep1a1326


title: VATTRTg_rep1a1332


title: VATTRTg_rep1a1329


title: VATTRTg_rep1a1320


title: VATTRTg_rep1a1318


title: VATTRTg_rep1a1344


title: VATTRTg_rep1a1317


title: VATTRTg_rep1a1354


title: VATTRTg_rep1a1319


title: VATTRTg_rep1a1314


title: VATTRTg_rep1a1309


title: VATTRTg_rep1a1311


title: VATTRTg_rep1a1305


title: VATTRTg_rep1a1302


title: VATTRTg_rep1a1300


title: VATTRTg_rep1a1336


title: VATTRTg_rep1a1304


title: VATTRTg_rep1a1306


title: VATTRTg_rep1a1313


title: VATTRTg_rep1a1310


title: VATTRTg_rep1a1316


title: VATTRTg_rep1a1312


title: VATTRTg_rep1a1298


title: VATTRTg_rep1a1294


title: VATTRTg_rep1a1307


title: VATTRTg_rep1a1289


title: VATTRTg_rep1a1290


title: VATTRTg_rep1a1284


title: VATTRTg_rep1a1301


title: VATTRTg_rep1a1299


title: VATTRTg_rep1a1303


title: VATTRTg_rep1a1297


title: VATTRTg_rep1a1288


title: VATTRTg_rep1a1292


title: VATTRTg_rep1a1291


title: VATTRTg_rep1a1293


title: VATTRTg_rep1a1295


title: VATTRTg_rep1a1276


title: VATTRTg_rep1a1277


title: VATTRTg_rep1a1275


title: VATTRTg_rep1a1263


title: VATTRTg_rep1a1270


title: VATTRTg_rep1a1271


title: VATTRTg_rep1a1267


title: VATTRTg_rep1a1262


title: VATTRTg_rep1a1281


title: VATTRTg_rep1a1278


title: VATTRTg_rep1a1282


title: VATTRTg_rep1a1280


title: VATTRTg_rep1a1257


title: VATTRTg_rep1a1279


title: VATTRTg_rep1a1252


title: VATTRTg_rep1a1274


title: VATTRTg_rep1a1246


title: VATTRTg_rep1a1285


title: VATTRTg_rep1a1265


title: VATTRTg_rep1a1264


title: VATTRTg_rep1a1273


title: VATTRTg_rep1a1236


title: VATTRTg_rep1a1235


title: VATTRTg_rep1a1255


title: VATTRTg_rep1a1249


title: VATTRTg_rep1a1254


title: VATTRTg_rep1a1232


title: VATTRTg_rep1a1234


title: VATTRTg_rep1a1260


title: VATTRTg_rep1a1230


title: VATTRTg_rep1a1240


title: VATTRTg_rep1a1238


title: VATTRTg_rep1a1228


title: VATTRTg_rep1a1248


title: VATTRTg_rep1a1225


title: VATTRTg_rep1a1229


title: VATTRTg_rep1a1237


title: VATTRTg_rep1a1213


title: VATTRTg_rep1a1214


title: VATTRTg_rep1a1211


title: VATTRTg_rep1a1212


title: VATTRTg_rep1a1256


title: VATTRTg_rep1a1231


title: VATTRTg_rep1a1222


title: VATTRTg_rep1a1217


title: VATTRTg_rep1a1251


title: VATTRTg_rep1a1216


title: VATTRTg_rep1a1205


title: VATTRTg_rep1a1224


title: VATTRTg_rep1a1223


title: VATTRTg_rep1a1195


title: VATTRTg_rep1a1196


title: VATTRTg_rep1a1194


title: VATTRTg_rep1a1197


title: VATTRTg_rep1a1210


title: VATTRTg_rep1a1201


title: VATTRTg_rep1a1233


title: VATTRTg_rep1a1203


title: VATTRTg_rep1a1207


title: VATTRTg_rep1a1209


title: VATTRTg_rep1a1200


title: VATTRTg_rep1a1199


title: VATTRTg_rep1a1204


title: VATTRTg_rep1a1184


title: VATTRTg_rep1a1202


title: VATTRTg_rep1a1181


title: VATTRTg_rep1a1193


title: VATTRTg_rep1a1198


title: VATTRTg_rep1a1191


title: VATTRTg_rep1a1192


title: VATTRTg_rep1a1188


title: VATTRTg_rep1a1167


title: VATTRTg_rep1a1165


title: VATTRTg_rep1a1164


title: VATTRTg_rep1a1186


title: VATTRTg_rep1a1187


title: VATTRTg_rep1a1174


title: VATTRTg_rep1a1176


title: VATTRTg_rep1a1175


title: VATTRTg_rep1a1189


title: VATTRTg_rep1a1170


title: VATTRTg_rep1a1166


title: VATTRTg_rep1a1172


title: VATTRTg_rep1a1156


title: VATTRTg_rep1a1151


title: VATTRTg_rep1a1163


title: VATTRTg_rep1a1162


title: VATTRTg_rep1a1150


title: VATTRTg_rep1a1161


title: VATTRTg_rep1a1173


title: VATTRTg_rep1a1158


title: VATTRTg_rep1a1152


title: VATTRTg_rep1a1154


title: VATTRTg_rep1a1145


title: VATTRTg_rep1a1147


title: VATTRTg_rep1a1149


title: VATTRTg_rep1a1146


title: VATTRTg_rep1a1140


title: VATTRTg_rep1a1155


title: VATTRTg_rep1a1136


title: VATTRTg_rep1a1133


title: VATTRTg_rep1a1137


title: VATTRTg_rep1a1132


title: VATTRTg_rep1a1142


title: VATTRTg_rep1a1141


title: VATTRTg_rep1a1144


title: VATTRTg_rep1a1128


title: VATTRTg_rep1a1131


title: VATTRTg_rep1a1138


title: VATTRTg_rep1a1135


title: VATTRTg_rep1a1139


title: VATTRTg_rep1a1127


title: VATTRTg_rep1a1123


title: VATTRTg_rep1a1121


title: VATTRTg_rep1a1134


title: VATTRTg_rep1a1119


title: VATTRTg_rep1a1117


title: VATTRTg_rep1a1126


title: VATTRTg_rep1a1129


title: VATTRTg_rep1a1125


title: VATTRTg_rep1a1120


title: VATTRTg_rep1a1113


title: VATTRTg_rep1a1124


title: VATTRTg_rep1a1116


title: VATTRTg_rep1a1109


title: VATTRTg_rep1a1143


title: VATTRTg_rep1a1130


title: VATTRTg_rep1a1103


title: VATTRTg_rep1a1106


title: VATTRTg_rep1a1098


title: VATTRTg_rep1a1093


title: VATTRTg_rep1a1095


title: VATTRTg_rep1a1090


title: VATTRTg_rep1a1107


title: VATTRTg_rep1a1099


title: VATTRTg_rep1a1097


title: VATTRTg_rep1a1100


title: VATTRTg_rep1a1081


title: VATTRTg_rep1a1110


title: VATTRTg_rep1a1087


title: VATTRTg_rep1a1088


title: VATTRTg_rep1a1082


title: VATTRTg_rep1a1083


title: VATTRTg_rep1a1078


title: VATTRTg_rep1a1108


title: VATTRTg_rep1a1094


title: VATTRTg_rep1a1118


title: VATTRTg_rep1a1091


title: VATTRTg_rep1a1092


title: VATTRTg_rep1a1076


title: VATTRTg_rep1a1073


title: VATTRTg_rep1a1074


title: VATTRTg_rep1a1085


title: VATTRTg_rep1a1084


title: VATTRTg_rep1a1075


title: VATTRTg_rep1a1070


title: VATTRTg_rep1a1079


title: VATTRTg_rep1a1062


title: VATTRTg_rep1a1055


title: VATTRTg_rep1a1045


title: VATTRTg_rep1a1072


title: VATTRTg_rep1a1057


title: VATTRTg_rep1a1047


title: VATTRTg_rep1a1066


title: VATTRTg_rep1a1059


title: VATTRTg_rep1a1065


title: VATTRTg_rep1a1069


title: VATTRTg_rep1a1080


title: VATTRTg_rep1a1048


title: VATTRTg_rep1a1049


title: VATTRTg_rep1a1043


title: VATTRTg_rep1a1053


title: VATTRTg_rep1a1058


title: VATTRTg_rep1a1040


title: VATTRTg_rep1a1071


title: VATTRTg_rep1a1042


title: VATTRTg_rep1a1039


title: VATTRTg_rep1a1046


title: VATTRTg_rep1a1061


title: VATTRTg_rep1a1044


title: VATTRTg_rep1a1051


title: VATTRTg_rep1a1038


title: VATTRTg_rep1a1056


title: VATTRTg_rep1a1041


title: VATTRTg_rep1a1037


title: VATTRTg_rep1a1054


title: VATTRTg_rep1a1035


title: VATTRTg_rep1a1052


title: VATTRTg_rep1a1031


title: VATTRTg_rep1a1034


title: VATTRTg_rep1a1029


title: VATTRTg_rep1a1023


title: VATTRTg_rep1a1036


title: VATTRTg_rep1a1015


title: VATTRTg_rep1a1017


title: VATTRTg_rep1a1014


title: VATTRTg_rep1a1010


title: VATTRTg_rep1a1007


title: VATTRTg_rep1a1008


title: VATTRTg_rep1a1011


title: VATTRTg_rep1a1020


title: VATTRTg_rep1a1018


title: VATTRTg_rep1a1027


title: VATTRTg_rep1a1021


title: VATTRTg_rep1a1025


title: VATTRTg_rep1a1024


title: VATTRTg_rep1a1003


title: VATTRTg_rep1a1012


title: VATTRTg_rep1a1013


title: VATTRTg_rep1a1002


title: VATTRTg_rep1a1009


title: VATTRTg_rep1a1006


title: VATTRTg_rep1a999


title: VATTRTg_rep1a1004


title: VATTRTg_rep1a1032


title: VATTRTg_rep1a990


title: VATTRTg_rep1a993


title: VATTRTg_rep1a1001


title: VATTRTg_rep1a1028


title: VATTRTg_rep1a989


title: VATTRTg_rep1a987


title: VATTRTg_rep1a1000


title: VATTRTg_rep1a994


title: VATTRTg_rep1a984


title: VATTRTg_rep1a977


title: VATTRTg_rep1a975


title: VATTRTg_rep1a978


title: VATTRTg_rep1a992


title: VATTRTg_rep1a991


title: VATTRTg_rep1a996


title: VATTRTg_rep1a972


title: VATTRTg_rep1a998


title: VATTRTg_rep1a973


title: VATTRTg_rep1a968


title: VATTRTg_rep1a981


title: VATTRTg_rep1a988


title: VATTRTg_rep1a985


title: VATTRTg_rep1a983


title: VATTRTg_rep1a966


title: VATTRTg_rep1a974


title: VATTRTg_rep1a964


title: VATTRTg_rep1a963


title: VATTRTg_rep1a997


title: VATTRTg_rep1a958


title: VATTRTg_rep1a959


title: VATTRTg_rep1a967


title: VATTRTg_rep1a965


title: VATTRTg_rep1a962


title: VATTRTg_rep1a960


title: VATTRTg_rep1a956


title: VATTRTg_rep1a976


title: VATTRTg_rep1a955


title: VATTRTg_rep1a951


title: VATTRTg_rep1a950


title: VATTRTg_rep1a949


title: VATTRTg_rep1a931


title: VATTRTg_rep1a971


title: VATTRTg_rep1a946


title: VATTRTg_rep1a934


title: VATTRTg_rep1a930


title: VATTRTg_rep1a925


title: VATTRTg_rep1a932


title: VATTRTg_rep1a923


title: VATTRTg_rep1a948


title: VATTRTg_rep1a945


title: VATTRTg_rep1a938


title: VATTRTg_rep1a939


title: VATTRTg_rep1a941


title: VATTRTg_rep1a926


title: VATTRTg_rep1a937


title: VATTRTg_rep1a936


title: VATTRTg_rep1a935


title: VATTRTg_rep1a927


title: VATTRTg_rep1a929


title: VATTRTg_rep1a924


title: VATTRTg_rep1a916


title: VATTRTg_rep1a917


title: VATTRTg_rep1a947


title: VATTRTg_rep1a913


title: VATTRTg_rep1a919


title: VATTRTg_rep1a920


title: VATTRTg_rep1a918


title: VATTRTg_rep1a910


title: VATTRTg_rep1a907


title: VATTRTg_rep1a911


title: VATTRTg_rep1a908


title: VATTRTg_rep1a905


title: VATTRTg_rep1a897


title: VATTRTg_rep1a898


title: VATTRTg_rep1a895


title: VATTRTg_rep1a904


title: VATTRTg_rep1a899


title: VATTRTg_rep1a903


title: VATTRTg_rep1a902


title: VATTRTg_rep1a887


title: VATTRTg_rep1a900


title: VATTRTg_rep1a891


title: VATTRTg_rep1a883


title: VATTRTg_rep1a890


title: VATTRTg_rep1a886


title: VATTRTg_rep1a912


title: VATTRTg_rep1a892


title: VATTRTg_rep1a882


title: VATTRTg_rep1a877


title: VATTRTg_rep1a875


title: VATTRTg_rep1a881


title: VATTRTg_rep1a880


title: VATTRTg_rep1a879


title: VATTRTg_rep1a873


title: VATTRTg_rep1a872


title: VATTRTg_rep1a874


title: VATTRTg_rep1a888


title: VATTRTg_rep1a906


title: VATTRTg_rep1a870


title: VATTRTg_rep1a884


title: VATTRTg_rep1a869


title: VATTRTg_rep1a868


title: VATTRTg_rep1a867


title: VATTRTg_rep1a863


title: VATTRTg_rep1a862


title: VATTRTg_rep1a864


title: VATTRTg_rep1a865


title: VATTRTg_rep1a852


title: VATTRTg_rep1a851


title: VATTRTg_rep1a866


title: VATTRTg_rep1a860


title: VATTRTg_rep1a858


title: VATTRTg_rep1a853


title: VATTRTg_rep1a859


title: VATTRTg_rep1a857


title: VATTRTg_rep1a850


title: VATTRTg_rep1a846


title: VATTRTg_rep1a849


title: VATTRTg_rep1a844


title: VATTRTg_rep1a845


title: VATTRTg_rep1a836


title: VATTRTg_rep1a838


title: VATTRTg_rep1a843


title: VATTRTg_rep1a839


title: VATTRTg_rep1a856


title: VATTRTg_rep1a831


title: VATTRTg_rep1a832


title: VATTRTg_rep1a837


title: VATTRTg_rep1a833


title: VATTRTg_rep1a830


title: VATTRTg_rep1a855


title: VATTRTg_rep1a828


title: VATTRTg_rep1a829


title: VATTRTg_rep1a825


title: VATTRTg_rep1a826


title: VATTRTg_rep1a824


title: VATTRTg_rep1a815


title: VATTRTg_rep1a823


title: VATTRTg_rep1a819


title: VATTRTg_rep1a814


title: VATTRTg_rep1a822


title: VATTRTg_rep1a811


title: VATTRTg_rep1a821


title: VATTRTg_rep1a816


title: VATTRTg_rep1a808


title: VATTRTg_rep1a810


title: VATTRTg_rep1a807


title: VATTRTg_rep1a842


title: VATTRTg_rep1a804


title: VATTRTg_rep1a803


title: VATTRTg_rep1a800


title: VATTRTg_rep1a802


title: VATTRTg_rep1a798


title: VATTRTg_rep1a827


title: VATTRTg_rep1a797


title: VATTRTg_rep1a794


title: VATTRTg_rep1a795


title: VATTRTg_rep1a796


title: VATTRTg_rep1a801


title: VATTRTg_rep1a790


title: VATTRTg_rep1a789


title: VATTRTg_rep1a791


title: VATTRTg_rep1a788


title: VATTRTg_rep1a793


title: VATTRTg_rep1a805


title: VATTRTg_rep1a780


title: VATTRTg_rep1a786


title: VATTRTg_rep1a776


title: VATTRTg_rep1a775


title: VATTRTg_rep1a781


title: VATTRTg_rep1a784


title: VATTRTg_rep1a779


title: VATTRTg_rep1a773


title: VATTRTg_rep1a782


title: VATTRTg_rep1a769


title: VATTRTg_rep1a770


title: VATTRTg_rep1a767


title: VATTRTg_rep1a792


title: VATTRTg_rep1a757


title: VATTRTg_rep1a787


title: VATTRTg_rep1a758


title: VATTRTg_rep1a766


title: VATTRTg_rep1a755


title: VATTRTg_rep1a759


title: VATTRTg_rep1a765


title: VATTRTg_rep1a763


title: VATTRTg_rep1a762


title: VATTRTg_rep1a764


title: VATTRTg_rep1a756


title: VATTRTg_rep1a753


title: VATTRTg_rep1a752


title: VATTRTg_rep1a778


title: VATTRTg_rep1a744


title: VATTRTg_rep1a748


title: VATTRTg_rep1a749


title: VATTRTg_rep1a742


title: VATTRTg_rep1a746


title: VATTRTg_rep1a745


title: VATTRTg_rep1a751


title: VATTRTg_rep1a741


title: VATTRTg_rep1a750


title: VATTRTg_rep1a743


title: VATTRTg_rep1a739


title: VATTRTg_rep1a738


title: VATTRTg_rep1a737


title: VATTRTg_rep1a740


title: VATTRTg_rep1a760


title: VATTRTg_rep1a761


title: VATTRTg_rep1a734


title: VATTRTg_rep1a736


title: VATTRTg_rep1a723


title: VATTRTg_rep1a727


title: VATTRTg_rep1a722


title: VATTRTg_rep1a728


title: VATTRTg_rep1a735


title: VATTRTg_rep1a726


title: VATTRTg_rep1a729


title: VATTRTg_rep1a731


title: VATTRTg_rep1a733


title: VATTRTg_rep1a720


title: VATTRTg_rep1a730


title: VATTRTg_rep1a715


title: VATTRTg_rep1a710


title: VATTRTg_rep1a716


title: VATTRTg_rep1a718


title: VATTRTg_rep1a711


title: VATTRTg_rep1a713


title: VATTRTg_rep1a714


title: VATTRTg_rep1a705


title: VATTRTg_rep1a707


title: VATTRTg_rep1a709


title: VATTRTg_rep1a703


title: VATTRTg_rep1a702


title: VATTRTg_rep1a719


title: VATTRTg_rep1a699


title: VATTRTg_rep1a700


title: VATTRTg_rep1a724


title: VATTRTg_rep1a688


title: VATTRTg_rep1a690


title: VATTRTg_rep1a692


title: VATTRTg_rep1a694


title: VATTRTg_rep1a696


title: VATTRTg_rep1a693


title: VATTRTg_rep1a683


title: VATTRTg_rep1a695


title: VATTRTg_rep1a682


title: VATTRTg_rep1a697


title: VATTRTg_rep1a681


title: VATTRTg_rep1a685


title: VATTRTg_rep1a708


title: VATTRTg_rep1a674


title: VATTRTg_rep1a673


title: VATTRTg_rep1a680


title: VATTRTg_rep1a669


title: VATTRTg_rep1a667


title: VATTRTg_rep1a668


title: VATTRTg_rep1a678


title: VATTRTg_rep1a670


title: VATTRTg_rep1a663


title: VATTRTg_rep1a664


title: VATTRTg_rep1a662


title: VATTRTg_rep1a656


title: VATTRTg_rep1a659


title: VATTRTg_rep1a689


title: VATTRTg_rep1a661


title: VATTRTg_rep1a652


title: VATTRTg_rep1a653


title: VATTRTg_rep1a654


title: VATTRTg_rep1a646


title: VATTRTg_rep1a645


title: VATTRTg_rep1a651


title: VATTRTg_rep1a647


title: VATTRTg_rep1a671


title: VATTRTg_rep1a641


title: VATTRTg_rep1a643


title: VATTRTg_rep1a677


title: VATTRTg_rep1a637


title: VATTRTg_rep1a634


title: VATTRTg_rep1a633


title: VATTRTg_rep1a631


title: VATTRTg_rep1a632


title: VATTRTg_rep1a630


title: VATTRTg_rep1a629


title: VATTRTg_rep1a635


title: VATTRTg_rep1a628


title: VATTRTg_rep1a625


title: VATTRTg_rep1a627


title: VATTRTg_rep1a655


title: VATTRTg_rep1a624


title: VATTRTg_rep1a623


title: VATTRTg_rep1a621


title: VATTRTg_rep1a620


title: VATTRTg_rep1a619


title: VATTRTg_rep1a618


title: VATTRTg_rep1a616


title: VATTRTg_rep1a614


title: VATTRTg_rep1a617


title: VATTRTg_rep1a640


title: VATTRTg_rep1a615


title: VATTRTg_rep1a636


title: VATTRTg_rep1a613


title: VATTRTg_rep1a609


title: VATTRTg_rep1a608


title: VATTRTg_rep1a605


title: VATTRTg_rep1a600


title: VATTRTg_rep1a610


title: VATTRTg_rep1a626


title: VATTRTg_rep1a602


title: VATTRTg_rep1a611


title: VATTRTg_rep1a603


title: VATTRTg_rep1a599


title: VATTRTg_rep1a607


title: VATTRTg_rep1a648


title: VATTRTg_rep1a597


title: VATTRTg_rep1a598


title: VATTRTg_rep1a596


title: VATTRTg_rep1a593


title: VATTRTg_rep1a594


title: VATTRTg_rep1a591


title: VATTRTg_rep1a589


title: VATTRTg_rep1a590


title: VATTRTg_rep1a588


title: VATTRTg_rep1a587


title: VATTRTg_rep1a584


title: VATTRTg_rep1a582


title: VATTRTg_rep1a583


title: VATTRTg_rep1a580


title: VATTRTg_rep1a579


title: VATTRTg_rep1a606


title: VATTRTg_rep1a601


title: VATTRTg_rep1a577


title: VATTRTg_rep1a578


title: VATTRTg_rep1a576


title: VATTRTg_rep1a572


title: VATTRTg_rep1a574


title: VATTRTg_rep1a575


title: VATTRTg_rep1a570


title: VATTRTg_rep1a568


title: VATTRTg_rep1a566


title: VATTRTg_rep1a565


title: VATTRTg_rep1a563


title: VATTRTg_rep1a560


title: VATTRTg_rep1a562


title: VATTRTg_rep1a559


title: VATTRTg_rep1a558


title: VATTRTg_rep1a581


title: VATTRTg_rep1a555


title: VATTRTg_rep1a557


title: VATTRTg_rep1a554


title: VATTRTg_rep1a553


title: VATTRTg_rep1a552


title: VATTRTg_rep1a551


title: VATTRTg_rep1a548


title: VATTRTg_rep1a547


title: VATTRTg_rep1a542


title: VATTRTg_rep1a543


title: VATTRTg_rep1a545


title: VATTRTg_rep1a541


title: VATTRTg_rep1a569


title: VATTRTg_rep1a540


title: VATTRTg_rep1a538


title: VATTRTg_rep1a539


title: VATTRTg_rep1a571


title: VATTRTg_rep1a537


title: VATTRTg_rep1a529


title: VATTRTg_rep1a535


title: VATTRTg_rep1a530


title: VATTRTg_rep1a526


title: VATTRTg_rep1a527


title: VATTRTg_rep1a525


title: VATTRTg_rep1a524


title: VATTRTg_rep1a523


title: VATTRTg_rep1a521


title: VATTRTg_rep1a520


title: VATTRTg_rep1a519


title: VATTRTg_rep1a517


title: VATTRTg_rep1a515


title: VATTRTg_rep1a513


title: VATTRTg_rep1a512


title: VATTRTg_rep1a516


title: VATTRTg_rep1a511


title: VATTRTg_rep1a509


title: VATTRTg_rep1a506


title: VATTRTg_rep1a507


title: VATTRTg_rep1a508


title: VATTRTg_rep1a536


title: VATTRTg_rep1a499


title: VATTRTg_rep1a500


title: VATTRTg_rep1a504


title: VATTRTg_rep1a501


title: VATTRTg_rep1a498


title: VATTRTg_rep1a505


title: VATTRTg_rep1a528


title: VATTRTg_rep1a494


title: VATTRTg_rep1a496


title: VATTRTg_rep1a492


title: VATTRTg_rep1a491


title: VATTRTg_rep1a490


title: VATTRTg_rep1a489


title: VATTRTg_rep1a486


title: VATTRTg_rep1a483


title: VATTRTg_rep1a482


title: VATTRTg_rep1a481


title: VATTRTg_rep1a487


title: VATTRTg_rep1a479


title: VATTRTg_rep1a488


title: VATTRTg_rep1a510


title: VATTRTg_rep1a478


title: VATTRTg_rep1a476


title: VATTRTg_rep1a474


title: VATTRTg_rep1a472


title: VATTRTg_rep1a497


title: VATTRTg_rep1a471


title: VATTRTg_rep1a467


title: VATTRTg_rep1a470


title: VATTRTg_rep1a475


title: VATTRTg_rep1a465


title: VATTRTg_rep1a463


title: VATTRTg_rep1a462


title: VATTRTg_rep1a469


title: VATTRTg_rep1a468


title: VATTRTg_rep1a458


title: VATTRTg_rep1a466


title: VATTRTg_rep1a456


title: VATTRTg_rep1a455


title: VATTRTg_rep1a457


title: VATTRTg_rep1a453


title: VATTRTg_rep1a454


title: VATTRTg_rep1a452


title: VATTRTg_rep1a449


title: VATTRTg_rep1a448


title: VATTRTg_rep1a451


title: VATTRTg_rep1a446


title: VATTRTg_rep1a447


title: VATTRTg_rep1a445


title: VATTRTg_rep1a477


title: VATTRTg_rep1a440


title: VATTRTg_rep1a441


title: VATTRTg_rep1a439


title: VATTRTg_rep1a442


title: VATTRTg_rep1a436


title: VATTRTg_rep1a460


title: VATTRTg_rep1a437


title: VATTRTg_rep1a438


title: VATTRTg_rep1a434


title: VATTRTg_rep1a430


title: VATTRTg_rep1a435


title: VATTRTg_rep1a433


title: VATTRTg_rep1a426


title: VATTRTg_rep1a424


title: VATTRTg_rep1a427


title: VATTRTg_rep1a420


title: VATTRTg_rep1a419


title: VATTRTg_rep1a417


title: VATTRTg_rep1a428


title: VATTRTg_rep1a413


title: VATTRTg_rep1a414


title: VATTRTg_rep1a411


title: VATTRTg_rep1a412


title: VATTRTg_rep1a410


title: VATTRTg_rep1a418


title: VATTRTg_rep1a408


title: VATTRTg_rep1a444


title: VATTRTg_rep1a409


title: VATTRTg_rep1a404


title: VATTRTg_rep1a405


title: VATTRTg_rep1a407


title: VATTRTg_rep1a406


title: VATTRTg_rep1a402


title: VATTRTg_rep1a403


title: VATTRTg_rep1a401


title: VATTRTg_rep1a425


title: VATTRTg_rep1a396


title: VATTRTg_rep1a397


title: VATTRTg_rep1a394


title: VATTRTg_rep1a393


title: VATTRTg_rep1a389


title: VATTRTg_rep1a387


title: VATTRTg_rep1a392


title: VATTRTg_rep1a385


title: VATTRTg_rep1a415


title: VATTRTg_rep1a384


title: VATTRTg_rep1a381


title: VATTRTg_rep1a383


title: VATTRTg_rep1a376


title: VATTRTg_rep1a374


title: VATTRTg_rep1a380


title: VATTRTg_rep1a373


title: VATTRTg_rep1a372


title: VATTRTg_rep1a369


title: VATTRTg_rep1a371


title: VATTRTg_rep1a370


title: VATTRTg_rep1a366


title: VATTRTg_rep1a390


title: VATTRTg_rep1a363


title: VATTRTg_rep1a391


title: VATTRTg_rep1a360


title: VATTRTg_rep1a362


title: VATTRTg_rep1a364


title: VATTRTg_rep1a361


title: VATTRTg_rep1a356


title: VATTRTg_rep1a353


title: VATTRTg_rep1a378


title: VATTRTg_rep1a365


title: VATTRTg_rep1a386


title: VATTRTg_rep1a350


title: VATTRTg_rep1a348


title: VATTRTg_rep1a346


title: VATTRTg_rep1a357


title: VATTRTg_rep1a382


title: VATTRTg_rep1a343


title: VATTRTg_rep1a342


title: VATTRTg_rep1a345


title: VATTRTg_rep1a358


title: VATTRTg_rep1a340


title: VATTRTg_rep1a339


title: VATTRTg_rep1a347


title: VATTRTg_rep1a336


title: VATTRTg_rep1a337


title: VATTRTg_rep1a338


title: VATTRTg_rep1a334


title: VATTRTg_rep1a332


title: VATTRTg_rep1a333


title: VATTRTg_rep1a331


title: VATTRTg_rep1a330


title: VATTRTg_rep1a329


title: VATTRTg_rep1a344


title: VATTRTg_rep1a326


title: VATTRTg_rep1a325


title: VATTRTg_rep1a327


title: VATTRTg_rep1a328


title: VATTRTg_rep1a321


title: VATTRTg_rep1a324


title: VATTRTg_rep1a320


title: VATTRTg_rep1a322


title: VATTRTg_rep1a341


title: VATTRTg_rep1a318


title: VATTRTg_rep1a312


title: VATTRTg_rep1a316


title: VATTRTg_rep1a314


title: VATTRTg_rep1a311


title: VATTRTg_rep1a317


title: VATTRTg_rep1a308


title: VATTRTg_rep1a310


title: VATTRTg_rep1a309


title: VATTRTg_rep1a307


title: VATTRTg_rep1a304


title: VATTRTg_rep1a306


title: VATTRTg_rep1a299


title: VATTRTg_rep1a300


title: VATTRTg_rep1a297


title: VATTRTg_rep1a295


title: VATTRTg_rep1a294


title: VATTRTg_rep1a319


title: VATTRTg_rep1a286


title: VATTRTg_rep1a289


title: VATTRTg_rep1a291


title: VATTRTg_rep1a285


title: VATTRTg_rep1a287


title: VATTRTg_rep1a274


title: VATTRTg_rep1a283


title: VATTRTg_rep1a272


title: VATTRTg_rep1a281


title: VATTRTg_rep1a270


title: VATTRTg_rep1a277


title: VATTRTg_rep1a278


title: VATTRTg_rep1a268


title: VATTRTg_rep1a279


title: VATTRTg_rep1a275


title: VATTRTg_rep1a284


title: VATTRTg_rep1a266


title: VATTRTg_rep1a276


title: VATTRTg_rep1a265


title: VATTRTg_rep1a264


title: VATTRTg_rep1a261


title: VATTRTg_rep1a298


title: VATTRTg_rep1a263


title: VATTRTg_rep1a259


title: VATTRTg_rep1a262


title: VATTRTg_rep1a257


title: VATTRTg_rep1a258


title: VATTRTg_rep1a255


title: VATTRTg_rep1a271


title: VATTRTg_rep1a253


title: VATTRTg_rep1a248


title: VATTRTg_rep1a249


title: VATTRTg_rep1a243


title: VATTRTg_rep1a239


title: VATTRTg_rep1a242


title: VATTRTg_rep1a232


title: VATTRTg_rep1a237


title: VATTRTg_rep1a233


title: VATTRTg_rep1a231


title: VATTRTg_rep1a236


title: VATTRTg_rep1a234


title: VATTRTg_rep1a235


title: VATTRTg_rep1a230


title: VATTRTg_rep1a238


title: VATTRTg_rep1a227


title: VATTRTg_rep1a228


title: VATTRTg_rep1a225


title: VATTRTg_rep1a229


title: VATTRTg_rep1a256


title: VATTRTg_rep1a224


title: VATTRTg_rep1a220


title: VATTRTg_rep1a218


title: VATTRTg_rep1a254


title: VATTRTg_rep1a216


title: VATTRTg_rep1a217


title: VATTRTg_rep1a247


title: VATTRTg_rep1a213


title: VATTRTg_rep1a215


title: VATTRTg_rep1a211


title: VATTRTg_rep1a209


title: VATTRTg_rep1a208


title: VATTRTg_rep1a206


title: VATTRTg_rep1a203


title: VATTRTg_rep1a205


title: VATTRTg_rep1a221


title: VATTRTg_rep1a194


title: VATTRTg_rep1a192


title: VATTRTg_rep1a191


title: VATTRTg_rep1a196


title: VATTRTg_rep1a200


title: VATTRTg_rep1a186


title: VATTRTg_rep1a190


title: VATTRTg_rep1a222


title: VATTRTg_rep1a185


title: VATTRTg_rep1a184


title: VATTRTg_rep1a199


title: VATTRTg_rep1a183


title: VATTRTg_rep1a182


title: VATTRTg_rep1a195


title: VATTRTg_rep1a212


title: VATTRTg_rep1a181


title: VATTRTg_rep1a197


title: VATTRTg_rep1a175


title: VATTRTg_rep1a178


title: VATTRTg_rep1a177


title: VATTRTg_rep1a179


title: VATTRTg_rep1a187


title: VATTRTg_rep1a173


title: VATTRTg_rep1a172


title: VATTRTg_rep1a169


title: VATTRTg_rep1a188


title: VATTRTg_rep1a167


title: VATTRTg_rep1a166


title: VATTRTg_rep1a165


title: VATTRTg_rep1a163


title: VATTRTg_rep1a160


title: VATTRTg_rep1a162


title: VATTRTg_rep1a156


title: VATTRTg_rep1a159


title: VATTRTg_rep1a155


title: VATTRTg_rep1a152


title: VATTRTg_rep1a154


title: VATTRTg_rep1a148


title: VATTRTg_rep1a149


title: VATTRTg_rep1a153


title: VATTRTg_rep1a145


title: VATTRTg_rep1a151


title: VATTRTg_rep1a144


title: VATTRTg_rep1a147


title: VATTRTg_rep1a143


title: VATTRTg_rep1a142


title: VATTRTg_rep1a138


title: VATTRTg_rep1a140


title: VATTRTg_rep1a141


title: VATTRTg_rep1a139


title: VATTRTg_rep1a171


title: VATTRTg_rep1a137


title: VATTRTg_rep1a168


title: VATTRTg_rep1a136


title: VATTRTg_rep1a135


title: VATTRTg_rep1a134


title: VATTRTg_rep1a133


title: VATTRTg_rep1a131


title: VATTRTg_rep1a130


title: VATTRTg_rep1a129


title: VATTRTg_rep1a128


title: VATTRTg_rep1a127


title: VATTRTg_rep1a126


title: VATTRTg_rep1a123


title: VATTRTg_rep1a122


title: VATTRTg_rep1a120


title: VATTRTg_rep1a124


title: VATTRTg_rep1a119


title: VATTRTg_rep1a121


title: VATTRTg_rep1a114


title: VATTRTg_rep1a113


title: VATTRTg_rep1a117


title: VATTRTg_rep1a116


title: VATTRTg_rep1a112


title: VATTRTg_rep1a109


title: VATTRTg_rep1a108


title: VATTRTg_rep1a107


title: VATTRTg_rep1a105


title: VATTRTg_rep1a115


title: VATTRTg_rep1a110


title: VATTRTg_rep1a104


title: VATTRTg_rep1a102


title: VATTRTg_rep1a98


title: VATTRTg_rep1a106


title: VATTRTg_rep1a99


title: VATTRTg_rep1a100


title: VATTRTg_rep1a96


title: VATTRTg_rep1a125


title: VATTRTg_rep1a94


title: VATTRTg_rep1a93


title: VATTRTg_rep1a111


title: VATTRTg_rep1a88


title: VATTRTg_rep1a89


title: VATTRTg_rep1a91


title: VATTRTg_rep1a90


title: VATTRTg_rep1a86


title: VATTRTg_rep1a82


title: VATTRTg_rep1a83


title: VATTRTg_rep1a85


title: VATTRTg_rep1a79


title: VATTRTg_rep1a80


title: VATTRTg_rep1a78


title: VATTRTg_rep1a77


title: VATTRTg_rep1a73


title: VATTRTg_rep1a76


title: VATTRTg_rep1a69


title: VATTRTg_rep1a71


title: VATTRTg_rep1a74


title: VATTRTg_rep1a67


title: VATTRTg_rep1a97


title: VATTRTg_rep1a68


title: VATTRTg_rep1a63


title: VATTRTg_rep1a64


title: VATTRTg_rep1a95


title: VATTRTg_rep1a62


title: VATTRTg_rep1a92


title: VATTRTg_rep1a60


title: VATTRTg_rep1a59


title: VATTRTg_rep1a57


title: VATTRTg_rep1a55


title: VATTRTg_rep1a61


title: VATTRTg_rep1a53


title: VATTRTg_rep1a52


title: VATTRTg_rep1a49


title: VATTRTg_rep1a54


title: VATTRTg_rep1a81


title: VATTRTg_rep1a48


title: VATTRTg_rep1a58


title: VATTRTg_rep1a65


title: VATTRTg_rep1a46


title: VATTRTg_rep1a50


title: VATTRTg_rep1a45


title: VATTRTg_rep1a41


title: VATTRTg_rep1a40


title: VATTRTg_rep1a39


title: VATTRTg_rep1a42


title: VATTRTg_rep1a51


title: VATTRTg_rep1a32


title: VATTRTg_rep1a35


title: VATTRTg_rep1a38


title: VATTRTg_rep1a31


title: VATTRTg_rep1a30


title: VATTRTg_rep1a27


title: VATTRTg_rep1a29


title: VATTRTg_rep1a28


title: VATTRTg_rep1a26


title: VATTRTg_rep1a25


title: VATTRTg_rep1a24


title: VATTRTg_rep1a20


title: VATTRTg_rep1a18


title: VATTRTg_rep1a21


title: VATTRTg_rep1a22


title: VATTRTg_rep1a14


title: VATTRTg_rep1a17


title: VATTRTg_rep1a13


title: VATTRTg_rep1a10


title: VATTRTg_rep1a4


title: VATTRTg_rep1a3


title: VATTRTg_rep1a12


title: VATTRTg_rep1a2


title: VATTR_rep2a2563

value: B6 Foxp3-Ires-Gfp

value: notTg


title: VATTRTg_rep1a1


title: VATTRTg_rep1a7


title: VATTR_rep2a2555


title: VATTRTg_rep1a8


title: VATTR_rep2a2556


title: VATTR_rep2a2561


title: VATTR_rep2a2547


title: VATTR_rep2a2554


title: VATTR_rep2a2562


title: VATTR_rep2a2553


title: VATTR_rep2a2546


title: VATTR_rep2a2545


title: VATTR_rep2a2552


title: VATTRTg_rep1a23


title: VATTR_rep2a2539


title: VATTR_rep2a2544


title: VATTR_rep2a2543


title: VATTRTg_rep1a5


title: VATTR_rep2a2532


title: VATTR_rep2a2530


title: VATTR_rep2a2536


title: VATTR_rep2a2560


title: VATTR_rep2a2529


title: VATTR_rep2a2524


title: VATTR_rep2a2523


title: VATTR_rep2a2520


title: VATTR_rep2a2517


title: VATTR_rep2a2502


title: VATTR_rep2a2506


title: VATTR_rep2a2505


title: VATTR_rep2a2493


title: VATTR_rep2a2503


title: VATTR_rep2a2491


title: VATTR_rep2a2510


title: VATTR_rep2a2497


title: VATTR_rep2a2487


title: VATTR_rep2a2488


title: VATTR_rep2a2486


title: VATTR_rep2a2538


title: VATTR_rep2a2480


title: VATTR_rep2a2484


title: VATTR_rep2a2533


title: VATTR_rep2a2477


title: VATTR_rep2a2476


title: VATTR_rep2a2473


title: VATTR_rep2a2483


title: VATTR_rep2a2518


title: VATTR_rep2a2514


title: VATTR_rep2a2469


title: VATTR_rep2a2471


title: VATTR_rep2a2470


title: VATTR_rep2a2512


title: VATTR_rep2a2467


title: VATTR_rep2a2464


title: VATTR_rep2a2472


title: VATTR_rep2a2501


title: VATTR_rep2a2450


title: VATTR_rep2a2445


title: VATTR_rep2a2460


title: VATTR_rep2a2447


title: VATTR_rep2a2442


title: VATTR_rep2a2441


title: VATTR_rep2a2453


title: VATTR_rep2a2435


title: VATTR_rep2a2446


title: VATTR_rep2a2434


title: VATTR_rep2a2440


title: VATTR_rep2a2439


title: VATTR_rep2a2432


title: VATTR_rep2a2433


title: VATTR_rep2a2430


title: VATTR_rep2a2427


title: VATTR_rep2a2425


title: VATTR_rep2a2415


title: VATTR_rep2a2419


title: VATTR_rep2a2461


title: VATTR_rep2a2410


title: VATTR_rep2a2409


title: VATTR_rep2a2429


title: VATTR_rep2a2431


title: VATTR_rep2a2404


title: VATTR_rep2a2408


title: VATTR_rep2a2412


title: VATTR_rep2a2416


title: VATTR_rep2a2413


title: VATTR_rep2a2399


title: VATTR_rep2a2407


title: VATTR_rep2a2397


title: VATTR_rep2a2398


title: VATTR_rep2a2392


title: VATTR_rep2a2402


title: VATTR_rep2a2391


title: VATTR_rep2a2384


title: VATTR_rep2a2386


title: VATTR_rep2a2383


title: VATTR_rep2a2390


title: VATTR_rep2a2376


title: VATTR_rep2a2380


title: VATTR_rep2a2377


title: VATTR_rep2a2382


title: VATTR_rep2a2373


title: VATTR_rep2a2369


title: VATTR_rep2a2362


title: VATTR_rep2a2366


title: VATTR_rep2a2375


title: VATTR_rep2a2363


title: VATTR_rep2a2371


title: VATTR_rep2a2361


title: VATTR_rep2a2360


title: VATTR_rep2a2356


title: VATTR_rep2a2393


title: VATTR_rep2a2403


title: VATTR_rep2a2355


title: VATTR_rep2a2349


title: VATTR_rep2a2352


title: VATTR_rep2a2345


title: VATTR_rep2a2354


title: VATTR_rep2a2341


title: VATTR_rep2a2353


title: VATTR_rep2a2348


title: VATTR_rep2a2339


title: VATTR_rep2a2344


title: VATTR_rep2a2331


title: VATTR_rep2a2330


title: VATTR_rep2a2327


title: VATTR_rep2a2326


title: VATTR_rep2a2324


title: VATTR_rep2a2325


title: VATTR_rep2a2321


title: VATTR_rep2a2315


title: VATTR_rep2a2357


title: VATTR_rep2a2317


title: VATTR_rep2a2313


title: VATTR_rep2a2322


title: VATTR_rep2a2358


title: VATTR_rep2a2309


title: VATTR_rep2a2308


title: VATTR_rep2a2310


title: VATTR_rep2a2304


title: VATTR_rep2a2303


title: VATTR_rep2a2307


title: VATTR_rep2a2300


title: VATTR_rep2a2299


title: VATTR_rep2a2297


title: VATTR_rep2a2294


title: VATTR_rep2a2298


title: VATTR_rep2a2290


title: VATTR_rep2a2288


title: VATTR_rep2a2336


title: VATTR_rep2a2291


title: VATTR_rep2a2328


title: VATTR_rep2a2285


title: VATTR_rep2a2275


title: VATTR_rep2a2274


title: VATTR_rep2a2278


title: VATTR_rep2a2284


title: VATTR_rep2a2277


title: VATTR_rep2a2273


title: VATTR_rep2a2323


title: VATTR_rep2a2268


title: VATTR_rep2a2269


title: VATTR_rep2a2265


title: VATTR_rep2a2271


title: VATTR_rep2a2262


title: VATTR_rep2a2260


title: VATTR_rep2a2263


title: VATTR_rep2a2301


title: VATTR_rep2a2258


title: VATTR_rep2a2257


title: VATTR_rep2a2256


title: VATTR_rep2a2253


title: VATTR_rep2a2252


title: VATTR_rep2a2248


title: VATTR_rep2a2244


title: VATTR_rep2a2242


title: VATTR_rep2a2243


title: VATTR_rep2a2281


title: VATTR_rep2a2238


title: VATTR_rep2a2232


title: VATTR_rep2a2231


title: VATTR_rep2a2229


title: VATTR_rep2a2226


title: VATTR_rep2a2227


title: VATTR_rep2a2225


title: VATTR_rep2a2223


title: VATTR_rep2a2215


title: VATTR_rep2a2211


title: VATTR_rep2a2217


title: VATTR_rep2a2214


title: VATTR_rep2a2218


title: VATTR_rep2a2210


title: VATTR_rep2a2205


title: VATTR_rep2a2204


title: VATTR_rep2a2202


title: VATTR_rep2a2201


title: VATTR_rep2a2251


title: VATTR_rep2a2247


title: VATTR_rep2a2196


title: VATTR_rep2a2195


title: VATTR_rep2a2191


title: VATTR_rep2a2189


title: VATTR_rep2a2188


title: VATTR_rep2a2186


title: VATTR_rep2a2181


title: VATTR_rep2a2184


title: VATTR_rep2a2182


title: VATTR_rep2a2183


title: VATTR_rep2a2180


title: VATTR_rep2a2224


title: VATTR_rep2a2178


title: VATTR_rep2a2175


title: VATTR_rep2a2176


title: VATTR_rep2a2213


title: VATTR_rep2a2173


title: VATTR_rep2a2170


title: VATTR_rep2a2168


title: VATTR_rep2a2169


title: VATTR_rep2a2161


title: VATTR_rep2a2163


title: VATTR_rep2a2152


title: VATTR_rep2a2167


title: VATTR_rep2a2154


title: VATTR_rep2a2157


title: VATTR_rep2a2160


title: VATTR_rep2a2142


title: VATTR_rep2a2143


title: VATTR_rep2a2139


title: VATTR_rep2a2141


title: VATTR_rep2a2137


title: VATTR_rep2a2136


title: VATTR_rep2a2129


title: VATTR_rep2a2133


title: VATTR_rep2a2126


title: VATTR_rep2a2127


title: VATTR_rep2a2124


title: VATTR_rep2a2122


title: VATTR_rep2a2121


title: VATTR_rep2a2117


title: VATTR_rep2a2118


title: VATTR_rep2a2116


title: VATTR_rep2a2114


title: VATTR_rep2a2113


title: VATTR_rep2a2107


title: VATTR_rep2a2131


title: VATTR_rep2a2145


title: VATTR_rep2a2112


title: VATTR_rep2a2105


title: VATTR_rep2a2101


title: VATTR_rep2a2111


title: VATTR_rep2a2099


title: VATTR_rep2a2103


title: VATTR_rep2a2094


title: VATTR_rep2a2091


title: VATTR_rep2a2096


title: VATTR_rep2a2098


title: VATTR_rep2a2087


title: VATTR_rep2a2119


title: VATTR_rep2a2084


title: VATTR_rep2a2085


title: VATTR_rep2a2076


title: VATTR_rep2a2082


title: VATTR_rep2a2080


title: VATTR_rep2a2069


title: VATTR_rep2a2071


title: VATTR_rep2a2073


title: VATTR_rep2a2067


title: VATTR_rep2a2066


title: VATTR_rep2a2062


title: VATTR_rep2a2063


title: VATTR_rep2a2056


title: VATTR_rep2a2059


title: VATTR_rep2a2057


title: VATTR_rep2a2055


title: VATTR_rep2a2060


title: VATTR_rep2a2050


title: VATTR_rep2a2049


title: VATTR_rep2a2088


title: VATTR_rep2a2054


title: VATTR_rep2a2052


title: VATTR_rep2a2045


title: VATTR_rep2a2048


title: VATTR_rep2a2053


title: VATTR_rep2a2047


title: VATTR_rep2a2086


title: VATTR_rep2a2044


title: VATTR_rep2a2075


title: VATTR_rep2a2078


title: VATTR_rep2a2040


title: VATTR_rep2a2036


title: VATTR_rep2a2043


title: VATTR_rep2a2037


title: VATTR_rep2a2028


title: VATTR_rep2a2031


title: VATTR_rep2a2030


title: VATTR_rep2a2035


title: VATTR_rep2a2027


title: VATTR_rep2a2026


title: VATTR_rep2a2019


title: VATTR_rep2a2017


title: VATTR_rep2a2020


title: VATTR_rep2a2022


title: VATTR_rep2a2025


title: VATTR_rep2a2013


title: VATTR_rep2a2016


title: VATTR_rep2a2023


title: VATTR_rep2a2012


title: VATTR_rep2a2005


title: VATTR_rep2a2006


title: VATTR_rep2a2015


title: VATTR_rep2a2004


title: VATTR_rep2a2008


title: VATTR_rep2a2042


title: VATTR_rep2a2009


title: VATTR_rep2a2003


title: VATTR_rep2a2002


title: VATTR_rep2a1998


title: VATTR_rep2a1997


title: VATTR_rep2a1991


title: VATTR_rep2a1990


title: VATTR_rep2a1987


title: VATTR_rep2a1985


title: VATTR_rep2a2001


title: VATTR_rep2a1996


title: VATTR_rep2a1993


title: VATTR_rep2a1983


title: VATTR_rep2a1982


title: VATTR_rep2a1980


title: VATTR_rep2a1981


title: VATTR_rep2a1979


title: VATTR_rep2a1976


title: VATTR_rep2a1977


title: VATTR_rep2a1975


title: VATTR_rep2a1968


title: VATTR_rep2a1969


title: VATTR_rep2a1966


title: VATTR_rep2a1972


title: VATTR_rep2a1965


title: VATTR_rep2a1960


title: VATTR_rep2a1971


title: VATTR_rep2a1961


title: VATTR_rep2a1956


title: VATTR_rep2a1957


title: VATTR_rep2a1954


title: VATTR_rep2a1992


title: VATTR_rep2a1958


title: VATTR_rep2a1953


title: VATTR_rep2a1984


title: VATTR_rep2a1950


title: VATTR_rep2a1947


title: VATTR_rep2a1940


title: VATTR_rep2a1937


title: VATTR_rep2a1936


title: VATTR_rep2a1924


title: VATTR_rep2a1922


title: VATTR_rep2a1934


title: VATTR_rep2a1925


title: VATTR_rep2a1933


title: VATTR_rep2a1929


title: VATTR_rep2a1959


title: VATTR_rep2a1914


title: VATTR_rep2a1915


title: VATTR_rep2a1913


title: VATTR_rep2a1907


title: VATTR_rep2a1909


title: VATTR_rep2a1906


title: VATTR_rep2a1905


title: VATTR_rep2a1904


title: VATTR_rep2a1900


title: VATTR_rep2a1943


title: VATTR_rep2a1899


title: VATTR_rep2a1898


title: VATTR_rep2a1939


title: VATTR_rep2a1895


title: VATTR_rep2a1892


title: VATTR_rep2a1935


title: VATTR_rep2a1890


title: VATTR_rep2a1888


title: VATTR_rep2a1883


title: VATTR_rep2a1917


title: VATTR_rep2a1911


title: VATTR_rep2a1881


title: VATTR_rep2a1908


title: VATTR_rep2a1877


title: VATTR_rep2a1873


title: VATTR_rep2a1863


title: VATTR_rep2a1862


title: VATTR_rep2a1865


title: VATTR_rep2a1866


title: VATTR_rep2a1861


title: VATTR_rep2a1868


title: VATTR_rep2a1869


title: VATTR_rep2a1859


title: VATTR_rep2a1855


title: VATTR_rep2a1849


title: VATTR_rep2a1860


title: VATTR_rep2a1853


title: VATTR_rep2a1894


title: VATTR_rep2a1848


title: VATTR_rep2a1843


title: VATTR_rep2a1858


title: VATTR_rep2a1847


title: VATTR_rep2a1839


title: VATTR_rep2a1840


title: VATTR_rep2a1841


title: VATTR_rep2a1845


title: VATTR_rep2a1837


title: VATTR_rep2a1834


title: VATTR_rep2a1833


title: VATTR_rep2a1831


title: VATTR_rep2a1830


title: VATTR_rep2a1828


title: VATTR_rep2a1827


title: VATTR_rep2a1826


title: VATTR_rep2a1821


title: VATTR_rep2a1813


title: VATTR_rep2a1812


title: VATTR_rep2a1824


title: VATTR_rep2a1811


title: VATTR_rep2a1814


title: VATTR_rep2a1819


title: VATTR_rep2a1809


title: VATTR_rep2a1805


title: VATTR_rep2a1799


title: VATTR_rep2a1801


title: VATTR_rep2a1804


title: VATTR_rep2a1800


title: VATTR_rep2a1798


title: VATTR_rep2a1793


title: VATTR_rep2a1789


title: VATTR_rep2a1786


title: VATTR_rep2a1783


title: VATTR_rep2a1781


title: VATTR_rep2a1780


title: VATTR_rep2a1776


title: VATTR_rep2a1785