title: intensive-care unit patient [14_03_2013_D11_2213154]


title: healthy subject [14_03_2013_C05_hv52]

status: Public on Jul 06 2015

type: RNA

city: Msida

first: Brendon

last: Scicluna

address: Msida campus

country: Malta

institute: University of Malta

description: blood gene expression data from healthy subjects and critically-ill patients.

hyb_protocol: Standard Affymetrix GeneAtlas® Hybridization, Wash, and Stain Kit.

scan_protocol: Standard GeneTitan® multi channel instrument.

data_processing: Raw data scans (.CEL files) were read into the R language and environment for statistical computing (version 2.15.1; R Foundation for Statistical Computing, Vienna, Austria; http://www.R-project.org/). Pre-processing and quality control was performed by using the Affy package version 1.36.1. Array data were background corrected by Robust Multi-array Average, quantiles-normalized and summarized by medianpolish using the expresso function. The resultant 49,386 probe intensities were filtered by means of a 0.5 variance cutoff using the genefilter method to recover 24,646 expressed probes. The occurrence of non-experimental chip effects was evaluated by means of the Surrogate Variable Analysis (R package version 3.4.0) and corrected by the empirical Bayes method ComBat.

label: biotin

molecule: total RNA

organism: Homo sapiens

source_name: whole blood

label_protocol: 100ng total RNA was labeled using standard GeneChip® 3' IVT Express Kit (Affymetrix)

growth_protocol: Whole blood was collected in PAXgene blood RNA tubes, mixed by inversion 10X and stored at -80C.

extract_protocol: Total RNA was isolated in accordance with the PAXgene blood RNA isolation (QIAGEN) procedure using the QIAcube workstation.

treatment_protocol: PAXgene blood RNA tubes were left at room temperaturee to defrost (4 hours) while mixing by inversion.

tag: gender

value: male

tag: age

tag: pneumonia diagnoses

tag: thrombocytopenia

value: healthy


title: intensive-care unit patient [14_03_2013_E11_440]

value: female

value: cap

value: discovery


title: healthy subject [14_03_2013_B10_hv40]


title: intensive-care unit patient [14_03_2013_B06_2952545]

value: validation


title: intensive-care unit patient [14_03_2013_D08_1973]


title: intensive-care unit patient [14_03_2013_F11_228]


title: healthy subject [14_03_2013_C02_hv56]


title: healthy subject [14_03_2013_A10_hv35]


title: intensive-care unit patient [14_03_2013_A09_399]


title: intensive-care unit patient [14_03_2013_C08_2753480]

value: no-cap


title: intensive-care unit patient [14_03_2013_A08_948]


title: healthy subject [13_03_2013_F12_hv32]


title: healthy subject [14_03_2013_A12_hv21]


title: intensive-care unit patient [13_03_2013_H02_2173]


title: intensive-care unit patient [13_03_2013_C10_2886199]


title: intensive-care unit patient [13_03_2013_F11_2862824]

value: DM


title: intensive-care unit patient [13_03_2013_A05_2311555]

value: ICUA


title: intensive-care unit patient [13_03_2013_C03_732]


title: intensive-care unit patient [13_03_2013_H06_2809208]


title: intensive-care unit patient [12_03_2013_H12_602]


title: intensive-care unit patient [13_02_2013_D08_1850]


title: intensive-care unit patient [13_02_2013_C12_338]


title: intensive-care unit patient [13_02_2013_B06_2262695]


title: intensive-care unit patient [13_03_2013_H10_821]


title: intensive-care unit patient [12_03_2013_E10_643]


title: intensive-care unit patient [07_03_2013_H01_1281]


title: intensive-care unit patient [12_03_2013_B04_2459139]


title: intensive-care unit patient [07_03_2013_G02_873]


title: intensive-care unit patient [13_02_2013_B10_1521]


title: intensive-care unit patient [13_02_2013_F06_1988]


title: intensive-care unit patient [12_03_2013_A07_2463040]


title: intensive-care unit patient [13_02_2013_A08_2138225]


title: healthy subject [13_03_2013_F09_hv45]


title: intensive-care unit patient [13_02_2013_F12_2246]


title: intensive-care unit patient [07_03_2013_G12_1272]


title: intensive-care unit patient [12_03_2013_G10_2028]


title: intensive-care unit patient [13_03_2013_D08_2151524]


title: intensive-care unit patient [13_02_2013_E04_882]


title: intensive-care unit patient [07_03_2013_F08_2757591]


title: intensive-care unit patient [07_03_2013_F02_1695]


title: intensive-care unit patient [07_03_2013_A04_1171]


title: intensive-care unit patient [07_03_2013_B10_2545687]


title: intensive-care unit patient [07_03_2013_C09_2684834]


title: intensive-care unit patient [06_02_2013_F11_1498]


title: intensive-care unit patient [05_03_2013_D01_383]


title: intensive-care unit patient [06_02_2013_E04_218]


title: intensive-care unit patient [06_02_2013_F04_2664986]


title: intensive-care unit patient [06_02_2013_H03_2731081]


title: intensive-care unit patient [05_03_2013_A03_2572276]


title: intensive-care unit patient [05_03_2013_E11_605]


title: intensive-care unit patient [05_03_2013_C04_2405776]


title: intensive-care unit patient [05_03_2013_C12_2123]


title: intensive-care unit patient [03_04_2013_H09_2480]


title: intensive-care unit patient [05_03_2013_H09_1052]


title: intensive-care unit patient [06_02_2013_G02_2709216]


title: intensive-care unit patient [05_03_2013_A10_408]


title: intensive-care unit patient [05_03_2013_G02_2106085]


title: intensive-care unit patient [06_02_2013_F12_2586738]


title: intensive-care unit patient [06_02_2013_C05_2095]


title: intensive-care unit patient [05_03_2013_C06_2404709]


title: intensive-care unit patient [07_03_2013_B09_2107380]


title: intensive-care unit patient [05_03_2013_F01_2177356]


title: intensive-care unit patient [05_03_2013_B05_2590193]


title: intensive-care unit patient [06_02_2013_F02_2669401]


title: intensive-care unit patient [03_04_2013_H02_2404]


title: intensive-care unit patient [07_03_2013_A11_2000]


title: intensive-care unit patient [05_03_2013_B12_2165]


title: intensive-care unit patient [03_04_2013_H05_544]


title: intensive-care unit patient [06_02_2013_G01_2392950]


title: healthy subject [03_04_2013_E09_hv59]


title: intensive-care unit patient [03_04_2013_B06_1793]


title: intensive-care unit patient [03_04_2013_G07_3147]


title: intensive-care unit patient [03_04_2013_C08_2736]


title: CS_standard_biol.rep3

status: Public on Sep 20 2013

city: Turku

first: Taina

last: Tyystjarvi

address: Pharmacity/Itäinen Pitkäkatu 4 C, 6th floor

department: Biochemistry

country: Finland

institute: University of Turku

hyb_protocol: 500 ng Cy3-labelled sample was hybridized overnight at 65 °C following instruction of Agilent's Gene Expression Hybridization kit. Washes was performed using wash buffers from Gene Expression Wash Pack (Agilent) and following the manufacturers instructions.

scan_protocol: Slides were scanned on the Agilent Technologies Scanner (G2565CA) using scan profile AgilentHD_GX_1Color (Agilent HD 1- color gene expression microarrays).

data_processing: The scanned images were analyzed with Agilent's Feature Extraction program version 10.7.3. using  protocol GE1_107_Sep09 and Grid 016989_D_F_20070606. gProcessedSignal value and SystematicName were used to get normalized signal intensities. Data were normalized using the quantile-method.

organism: Synechocystis sp. PCC 6803

source_name: control strain_standard growth conditions

label_protocol: Cyanine-3 (Cy3) labeled cDNA was prepared from 7 μg of total RNA using One-Color Microarray-Based Prokaryote Analysis (FairPlay III Labeling) Protocol Version 1.3 (August 2010) (Agilent). Agilent’s RNA Spike-In Kit was added to the samples allowing to monitor microarray workflow. Dye incorporation and cDNA concentration were measured with the NanoDrop ND-2000 Spectrophotometer.

growth_protocol: Cell were grown in BG-11 medium in standard growth conditions (continuous illumination at the PPFD of 40 µmol m-2s-1, 32°C, ambient CO2)

extract_protocol: RNA isolation was done as described previously (Eisenhut, M., von Wobeser, E.A., Jonas, L., Schubert, H., Ibelings, B.W., Bauwe, H. et al. (2007) Plant Physiol. 144: 1946-1959). RNA was purified and DNase treated using RNeasy mini kit (Qiagen).

treatment_protocol: Cells grown in standard growth conditions (A730=1, 40 mL) were illuminated for 30-min at PPFD of 750 µmol m-2s-1.

tag: genotype/variation

value: wild type/control

tag: phenotype

value: glucose tolerant

tag: growth condition

value: standard condition


title: healthy subject [03_04_2013_F10_hv49]


title: ΔsigCDE_standard_biol.rep1

source_name: mutant ΔsigCDE_standard growth conditions

value: ΔsigCDE mutant


title: intensive-care unit patient [03_04_2013_B07_3252]


title: ΔsigCDE_standard_biol.rep2


title: ΔsigCDE_standard_biol.rep3


title: CS_standard_biol.rep4


title: CS_standard_biol.rep1


title: intensive-care unit patient [03_04_2013_F02_2618]


title: intensive-care unit patient [03_04_2013_B08_3242]


title: CS_standard_biol.rep2


title: intensive-care unit patient [03_04_2013_B02_3270]


title: intensive-care unit patient [03_04_2013_E06_526]


title: Somatic.Tissue__Adrenal__Male_Adult__Patient1_95.29.1.7

status: Public on May 14 2012

type: genomic

city: La Jolla

first: Kristopher

middle: L.

last: Nazor

state: CA

address: 10550 North Torrey Pines Road

department: Regenerative Medicine

country: USA

institute: The Scripps Research Institute

hyb_protocol: bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450k Beadchip using standard Illumina protocol

scan_protocol: Arrays were imaged using HiScan using standard recommended Illumina scanner setting

data_processing: Genome Studio software v2010.3

label: Cy5 and Cy3

molecule: genomic DNA

label_protocol: Standard Illumina Protocol

extract_protocol: genomic DNA was extracted and purified from LBC samples using Qiagen DNeasy Kit according to standard instructions

tag: loring pipeline sample id

tag: loring pipeline stem cell matrix version

value: stem cell matrix version 342

tag: cell type

tag: tissue type

value: Adrenal

tag: fetal vs adult tissue

value: Adult

tag: patient id

value: Male


title: Somatic.Tissue__Adrenal__Male_Fetal_20wk_Patient248_95.1440.1.1

value: Fetal

tag: gestational age


title: Somatic.Tissue__Skeletal.Muscle__Female_Adult__Patient2_97.30.1.1

value: Female


title: Somatic.Tissue__Lung__Male_Fetal_15wk_Patient30_94.412.1.1

value: Lung


title: Somatic.Tissue__Adrenal__Female_Fetal_14wk_Patient32_95.414.1.1


title: Somatic.Tissue__Lung__Female_Fetal_20wk_Patient250_94.1442.1.1


title: Somatic.Tissue__Lung__Female_Adult__Patient2_94.30.1.1


title: Somatic.Tissue__Skeletal.Muscle__Male_Adult__Patient1_97.29.1.7


title: Somatic.Tissue__Ureter__Female_Adult__Patient2_92.30.1.1

value: Ureter


title: Somatic.Tissue__Lung__Male_Adult__Patient1_94.29.1.7


title: Somatic.Tissue__Bladder__Male_Adult__Patient1_99.29.1.7

value: Bladder


title: Somatic.Tissue__Spleen__Female_Adult__Patient2_91.30.1.1

value: Spleen


title: Somatic.Tissue__Lung__Male_Fetal_20wk_Patient248_94.1440.1.1


title: Somatic.Tissue__Spleen__Female_Fetal_18wk_Patient249_91.1441.1.1


title: Somatic.Tissue__Ureter__Male_Adult__Patient1_92.29.1.2


title: Somatic.Tissue__Spleen__Female_Fetal_14wk_Patient32_91.414.1.1


title: Somatic.Tissue__Lung__Female_Fetal_18wk_Patient249_94.1441.1.1


title: Somatic.Tissue__Spleen__Male_Adult__Patient1_91.29.1.7


title: Somatic.Tissue__Lung__Female_Fetal_14wk_Patient32_94.414.1.1


title: Somatic.Tissue__Adrenal__Male_Fetal_15wk_Patient30_95.412.1.2


title: Somatic.Tissue__Pancreas__Male_Adult__Patient1_220.29.1.6

value: Pancreas


title: Somatic.Tissue__Brain__Female_Fetal_20wk_Patient250_21.1442.1.1

value: Brain


title: Somatic.Tissue__Brain__Female_Adult__Patient71_21.625.2.3


title: Somatic.Tissue__Brain__Female_Fetal_18wk_Patient249_21.1441.1.1


title: Somatic.Tissue__Thymus__Female_Fetal_20wk_Patient250_551.1442.1.1

value: Thymus


title: Somatic.Tissue__Spleen__Male_Fetal_20wk_Patient248_91.1440.1.1


title: Somatic.Tissue__Brain__Male_Fetal_15wk_Patient30_21.412.1.1


title: Somatic.Tissue__Pancreas__Female_Adult__Patient2_220.30.1.1


title: Somatic.Tissue__Thymus__Male_Fetal_20wk_Patient248_551.1440.1.1


title: Somatic.Tissue__Adrenal__Female_Adult__Patient2_95.30.2.1


title: Somatic.Tissue__Brain__Male_Fetal_20wk_Patient248_21.1440.1.1


title: Somatic.Tissue__Liver__Female_Fetal_18wk_Patient249_147.1441.1.1

value: Liver


title: Somatic.Tissue__Kidney__Female_Fetal_14wk_Patient32_143.414.1.1

value: Kidney


title: Somatic.Tissue__Heart__Female_Fetal_20wk_Patient250_111.1442.1.1

value: Heart


title: Somatic.Tissue__Liver__Female_Fetal_14wk_Patient32_147.414.1.1


title: Somatic.Tissue__Kidney__Male_Fetal_15wk_Patient30_143.412.1.1


title: Somatic.Tissue__Kidney__Female_Fetal_18wk_Patient249_143.1441.1.1


title: Somatic.Tissue__Blood__Female_Adult__Patient5_107.362.1.1

value: Blood


title: Somatic.Tissue__Tongue__Female_Fetal_14wk_Patient26_139.407.1.2

value: stem cell matrix version 342

value: Tongue


title: Somatic.Tissue__Stomach__Male_Fetal_15wk_Patient30_101.412.1.1

value: Stomach


title: Somatic.Tissue__Heart__Male_Fetal_20wk_Patient248_111.1440.1.1

value: Male


title: Somatic.Tissue__Kidney__Male_Fetal_20wk_Patient248_143.1440.1.1

value: Fetal


title: Somatic.Tissue__Blood__Female_Adult__Patient6_107.363.2.1

value: Adult

value: Female


title: Somatic.Tissue__Lymph.Node__Female_Adult__Patient2_100.30.1.1


title: Somatic.Tissue__Stomach__Female_Fetal_14wk_Patient26_101.407.1.2


title: Somatic.Tissue__Stomach__Female_Fetal_14wk_Patient32_101.414.1.1


title: Somatic.Tissue__Liver__Male_Fetal_20wk_Patient248_147.1440.1.1


title: Somatic.Tissue__Liver__Female_Fetal_20wk_Patient250_147.1442.1.1


title: Somatic.Tissue__Lymph.Node__Male_Adult__Patient1_100.29.1.3


title: Somatic.Primary__KDIB2__Male____251.669.1.2

tag: cell line


title: Somatic.Tissue__Heart__Female_Fetal_14wk_Patient32_111.414.1.1


title: DMS-53 Human Lung Cancer Cell Line - af1c1031-Dp44mT set1

status: Public on Dec 31 2011

city: Sydney

first: Des

middle: Raymond

last: Richardson

state: New South Wales

address: 92-94 Parramatta Rd, Sydney

department: Bosch Institute

country: Australia

institute: University of Sydney

description: Standard Affymetrix Protocol on total RNA extracted from DMS-53 cell line

hyb_protocol: Standard Affymetrix Protocol

scan_protocol: Standard Affymetrix Protocol, GeneChip Scanner 3000

data_processing: GeneChip Scanner 3000, Affymetrix Expression Console 1.1, affymetrix-algorithm-name = plier-mm, affymetrix-algorithm-version = 2.0

label: Biotin

source_name: DMS-53 cell line

label_protocol: Standard Affymetrix Protocol

extract_protocol: Standard TRIzol RNA Extraction Protocol

treatment_protocol: With 25 micromolar Dp44mT in RPMI media

tag: agent


title: Somatic.Tissue__Stomach__Male_Fetal_20wk_Patient248_101.1440.1.1


title: Somatic.Primary__HRCEpiC255__Male____210.563.1.1


title: Somatic.Primary__HESMC267__Male____207.560.1.1


title: Somatic.Primary__HRPTEpiC257__Male____209.562.1.1


title: Somatic.Tissue__Stomach__Female_Fetal_18wk_Patient249_101.1441.1.1


title: Somatic.Tissue__Heart__Female_Fetal_18wk_Patient249_111.1441.1.1


title: Somatic.Primary__HREpiC256__Male____211.564.1.1


title: Somatic.Tissue__Tongue__Female_Fetal_20wk_Patient250_139.1442.1.1


title: Somatic.Primary__HBVSMC266__Male____203.556.1.1


title: Somatic.Tissue__Bladder__Female_Adult__Patient2_99.30.1.1


title: DMS-53 Human Lung Cancer Cell Line - af1c1031-con set1

treatment_protocol: With Normal Treatment of RPMI media (Control)

value: RPMI media (control)


title: DC.4+.Sp.ST#4

status: Public on Oct 08 2010

city: Boston

first: Liang

last: Yang

state: MA

address: 77 Avenue Louis Pasteur

department: Pathology

institute: Harvard Medical School

description: DC.4+.Sp#4, CD4 CDs

hyb_protocol: Affy 1.0 ST Hyb Kit

scan_protocol: Affymetrix GeneChip Scanner

data_processing: The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.

organism: Mus musculus

source_name: spleen

label_protocol: Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit

extract_protocol: Collagenase, dispase, DNAse digestion, CD3e, CD19, GR1, NK1.1 MACS depletion

value: 6 weeks

tag: exclusion markers

value: PI SA-PECy7

tag: genetic background

tag: phenotype markers

value: CD11b CD4 CD45 CD11c CD8a

tag: separation method

value: dissection/trimming

tag: Sex

value: male


title: DC.8+.Sp.ST#7

description: DC.8+.Sp#7, CD8 DCs

extract_protocol: Liberase Blendzymes III, DNAse I digestion, CD3e, CD19, GR1, NK1.1 MACS depletion

tag: age

value: CD11b CD45 CD4 CD11c CD8a


title: DMS-53 Human Lung Cancer Cell Line - af1c1031-DFO set2

treatment_protocol: With DFO 250 micromolar in RPMI media

value: DFO


title: DMS-53 Human Lung Cancer Cell Line - af1c1031-DFO set1

treatment_protocol: With 250 micromolar DFO in RPMI media


title: DMS-53 Human Lung Cancer Cell Line - af1c1031-Dp44mT set2

treatment_protocol: With Dp44mT 25 micromolar in RPMI media


title: DMS-53 Human Lung Cancer Cell Line - af1c1031-con set2

treatment_protocol: With normal control RPMI media


title: Somatic.Primary__HBdSMC265__Male____212.565.1.1

tag: gender


title: T.4Nve.Sp#6

status: Public on Apr 29 2010

description: T.4Nve.Sp#6, Spleen Naive CD4

extract_protocol: Spleen crushed with syringe plunger


title: DC.8+.Sp.ST#8

description: DC.8+.Sp#8, spleen CD8+ dendritic cell

extract_protocol: Liberase Blendzymes III, DNAse I digestion  CD3e, CD19, GR1, NK1.1 MACS depletion

value: CD11b CD11c CD45 CD8a CD4


title: T.4Nve.Sp#8

description: T.4Nve.Sp#8, CD4 naive


title: T.4Nve.Sp#5

description: T.4Nve.Sp#5, Spleen Naive CD4


title: T.8Nve.Sp#1

description: T.8Nve.Sp#1, Spleen Naive CD8

extract_protocol: Glass slide teasing,  FACS sorting


title: DC.8+.Sp.ST#9

description: DC.8+.Sp#9, spleen CD8+ dendritic cell

extract_protocol: Liberase Blendzymes III, DNAse I digestion  CD3, CD19, GR1, NK1.1 MACS depletion


title: T.4Nve.Sp#7

description: T.4Nve.Sp#7, Spleen Naive CD4


title: GN.Bl#2

status: Public on Dec 28 2011

description: GN.Bl#2, GN.Bl

source_name: blood

extract_protocol: Lysed Blood

value: CD11b+ Ly6-G+


title: T.8Nve.Sp#3

description: T.8Nve.Sp#3, Spleen Naive CD8


title: DC.4+.Sp.ST#8

description: DC.4+.Sp#8, spleen CD4+ dendritic cell

extract_protocol: Liberase Blendzymes III, DNAse I digestion  CD3e, CD19, GR1, NK1.1


title: Somatic.Primary__KERA1__Male____137.400.3.1

value: stem cell matrix version 342


title: GN.Bl#1

description: GN.Bl#1, GN.Bl


title: B.MZ.Sp#1

description: B.MZ.Sp#1, MZ (Marginal Zone)

extract_protocol: Ground between 2 glass slides, ACK lysis of RBCs

value: 7 weeks

value: CD3 Ly6c Ter119 Gr-1 Mac1


title: DC.4+.Sp.ST#7

description: DC.4+.Sp#7, spleen CD4+ dendritic cell


title: B.MZ.Sp#2

description: B.MZ.Sp#2, MZ (Marginal Zone)

value: CD3 Ly6c Ter119 Gr-1 CD11b


title: DC.8+.Sp.ST#5

description: DC.8+.Sp#5, spleen CD8+ dendritic cell

extract_protocol: collagenase, dispase, DNAse digestion  CD3e, CD19, Gr1, NK1.1 MACS depletion


title: B.GC.Sp#3

description: B.GC.Sp#3, Germinal Center B cells, Spleen

extract_protocol: ground between 2 glass slides, ACK lysis of RBCs  2x flow sorting

value: 10 weeks

value: Ly6c Ter119 CD3 Gr-1 Cd11b

value: CD19+ IgM+ IgD- GL7+ PNA+


title: B.MZ.Sp#3

description: B.MZ.Sp#3, MZ (Marginal Zone)


title: DC.4+.Sp.ST#5

description: DC.4+.Sp#5, spleen CD4+ dendritic cell

extract_protocol: collagenase, dispase, DNAse digestion  CD3e, CD19, GR1, NK1.1 MACS depletion


title: GN.Bl#3

description: GN.Bl#3, GN.Bl


title: DC.4+.Sp.ST#9

description: DC.4+.Sp#9, spleen CD4+ dendritic cell


title: B.Fo.Sp#3

description: B.Fo.Sp#3, Fo (follicular)


title: DC.8+.Sp.ST#6

description: DC.8+.Sp#6, spleen CD8+ dendritic cell


title: B.GC.Sp#1

description: B.GC.Sp#1, Germinal Center B cells, Spleen

extract_protocol: grind between glass slides, ACK lysis of RBCs  2X flow sorting

value: Ter119 Ly6c CD3 CD11b Gr-1


title: B.Fo.Sp#1

description: B.Fo.Sp#1, Fo (follicular)


title: B.GC.Sp#2

description: B.GC.Sp#2, Germinal Center B cells, Spleen

value: 11 weeks


title: B.Fo.Sp#2

description: B.Fo.Sp#2, Fo (follicular)


title: Brain_C57 Wildtype_affs275-1099

status: Public on Mar 29 2006

description: Standard Affymetrix Protocol on total RNA extracted from brain tissue

data_processing: GeneChip Scanner 3000, GCOS v1.3, scaled to 1000

source_name: C57 Wildtype Mouse #1099 Brain

tag: Strain

value: C57BL/6 Gender:female Age:123 days Tissue:brain


title: Brain_C57 Wildtype_affs275-1100

source_name: C57 Wildtype Mouse #1100 Brain


title: MCF-7 Human Breast Cancer Cell Line - affs196-mem

status: Public on Feb 28 2010

description: Standard Affymetrix Protocol on total RNA extracted from MCF-7 cell line

data_processing: GeneChip Scanner 3000, GCOS v1.3

source_name: MCF-7 cell line

treatment_protocol: With Normal Treatment of MEM media (Control)

value: MEM media (control)


title: MCF-7 Human Breast Cancer Cell Line - affs196-dfo

treatment_protocol: Treated with Desferrioxamine at 250 uM for 24 hour

value: Desferrioxamine

tag: concentration

tag: time

value: 24 hour


title: MCF-7 Human Breast Cancer Cell Line - affs196-311

treatment_protocol: Treated with 2-hydroxy-1-napthylaldehyde isonicotinoyl hydrazone (311) at 25 uM for 24 hour

value: 25 uM


title: T.8Nve.Sp#2

description: T.8Nve.Sp#2, Spleen Naive CD8

value: 6 weeks

value: dissection/trimming

value: male


title: Brain_Melanotransferrin Knockout_affs275-1098

source_name: Melanotransferrin Knockout Mouse #1098 Brain

value: C57BL/6 - Lucy Gender:female Age:123 days Tissue:brain


title: Brain_Melanotransferrin Knockout_affs275-1096

source_name: Melanotransferrin Knockout Mouse #1096 Brain

value: C57BL/6 - Lucy

tag: Gender

value: female

tag: Age

value: 123 days

tag: Tissue

value: brain


title: Brain_Melanotransferrin Knockout_affs275-1101

source_name: Melanotransferrin Knockout Mouse #1101 Brain


title: Brain_Melanotransferrin Knockout_affs275-1097

source_name: Melanotransferrin Knockout Mouse #1097 Brain