title: MC002_ATH1_A7.3-dubos-wLh

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 131.51
Background Stdev (float) = 4.49
Background Max (float) = 144.7
Background Min (float) = 122.6
Noise Avg (float) = 7.31
Noise Stdev (float) = 0.35
Noise Max (float) = 8.5
Noise Min (float) = 6.6
RawQ (float) = 4.43
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 281
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: MC002_ATH1_A7.3-dubos-wLh.DAT

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 230 (type integer); Parameter Upper left corner y coordinate = 232 (type integer); Parameter Upper right corner x coordinate = 4499 (type integer); Parameter Upper right corner y coordinate = 261 (type integer); Parameter Lower right corner x coordinate = 4472 (type integer); Parameter Lower right corner y coordinate = 4531 (type integer); Parameter Lower left corner x coordinate = 202 (type integer); Parameter Lower left corner y coordinate = 4502 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 0.193891 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;


title: MC002_ATH1_A2.2-dubos-wtc

status: Public on Jan 22 2007

type: RNA

city: Loughborough

last: Nottingham Arabidopsis Stock Centre (NASC)

address: Sutton Bonington Campus

department: School of Biosciences, University of Nottingham

country: United Kingdom

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 75.52
Background Stdev (float) = 2.22
Background Max (float) = 81.6
Background Min (float) = 71.5
Noise Avg (float) = 3.71
Noise Stdev (float) = 0.16
Noise Max (float) = 4.2
Noise Min (float) = 3.3
RawQ (float) = 2.75
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 523
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: MC002_ATH1_A2.2-dubos-wtc.DAT

hyb_protocol: EukGE-WS2v4; Protocol Type = Hybridization; Parameter Wash A1 Recovery Mixes = 0 (type integer); Parameter Wash A1 Temperature (C) = 25 (type integer); Parameter Number of Wash A1 Cycles = 10 (type integer); Parameter Mixes per Wash A1 Cycle = 2 (type integer); Parameter Wash B Recovery Mixes = 0 (type integer); Parameter Wash B Temperature (C) = 50 (type integer); Parameter Number of Wash B Cycles = 4 (type integer); Parameter Mixes per Wash B Cycle = 15 (type integer); Parameter Stain Temperature (C) = 25 (type integer); Parameter First Stain Time (seconds) = 600 (type integer); Parameter Wash A2 Recovery Mixes = 0 (type integer); Parameter Wash A2 Temperature (C) = 25 (type integer); Parameter Number of Wash A2 Cycles = 10 (type integer); Parameter Mixes per Wash A2 Cycle = 4 (type integer); Parameter Second Stain Time (seconds) = 600 (type integer); Parameter Third Stain Time (seconds) = 600 (type integer); Parameter Wash A3 Recovery Mixes = 0 (type integer); Parameter Wash A3 Temperature (C) = 30 (type integer); Parameter Number of Wash A3 Cycles = 15 (type integer); Parameter Mixes per Wash A3 Cycle = 4 (type integer); Parameter Holding Temperature (C) = 25 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Fluidics Station, Parameter: Parameter Station Number = 1 (type integer), Parameter: Parameter Position = 3 (type integer); Performers: John,Y,Okyere, , Janet,,Higgins

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 231 (type integer); Parameter Upper left corner y coordinate = 231 (type integer); Parameter Upper right corner x coordinate = 4499 (type integer); Parameter Upper right corner y coordinate = 232 (type integer); Parameter Lower right corner x coordinate = 4498 (type integer); Parameter Lower right corner y coordinate = 4502 (type integer); Parameter Lower left corner x coordinate = 230 (type integer); Parameter Lower left corner y coordinate = 4501 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 0.348500 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;

label: biotin

molecule: total RNA

organism: Arabidopsis thaliana

label_protocol: Preparation of biotin-labeled cRNA; RNA samples were quality controlled using the Agilent 2100 Bioanalyzer. 100 pmol T7-(dT)24 primer (5'-GGCCAGTGAATTGTAATACGACTCACTATAGGGAGGCGG-(dT)24-3')  was hybridized with the Total RNA ( 8 - 12ug) at 70°C for 10 minutes. First strand cDNA was synthesized by reverse transcription using 400 units SuperScript II Reverse Transcriptase in a reaction containing 50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl2, 10 mM dithiothreitol (DTT) and 0.5 mM dNTPs at 42C for 1 hour.  The reaction was then put on ice for 2 mins, followed by second strand cDNA synthesis.  130ul of the second strand reaction mix was added to the 20ul  first strand synthesis reaction. The second strand reaction mix  contained 40 units E. coli DNA polymerase I, 10 units E. coli DNA ligase, 2 units  E. coli RNase H and 0.2 mM dNTPs in a buffer containing 20 mMTris-HCl (pH 6.9), 90 mM KCl, 4.6 mM MgCl2, 10mM (NH4)SO4 and 0.15 mM ß-NAD+. The reaction was incubated at 16 C for 2 hours.  10 units T4 DNA Polymerase was added and the reaction incubated for a further 5 minutes at 16 C, then the reaction was terminated using EDTA.  The double-stranded cDNA was cleaned up using the cDNA Cleanup Spin Column supplied in the Affymetrix GeneChip Sample Cleanup Module.  The double-stranded cDNA was used as a template for in Vitro transcription of biotin-labeled cRNA using the ENZO BioArray RNA Transcript Labeling Kit supplied by Affymetrix.  The Transcript Labeling kit produces large amounts of hybridizable biotin-labeled RNA targets by in Vitro transcription from bacteriophage T7 RNA polymerase promoters.  The reaction mixture containing Biotin-Labeled ribonucleotides (ATP, GTP, CTP, UTP with Bio-UTP and Bio-CTP), DTT, RNase inhibitor mix and T7 RNA polymerase was incubated at 37 C for 5 hrs, gently mixing every 30 minutes during the incubation. This produced an average of 40ug biotin-labeled cRNA . The biotin-labeled cRNA  was cleaned up using the cRNA Cleanup Spin Column supplied in the Affymetrix GeneChip Sample Cleanup Module.  The quantity and quality of the cRNA was measured using the Agilent 2100 Bioanalyzer. The total amount of cRNA was calculated, the starting quantity of RNA subtracted to give the quantity of adjusted cRNA.  The cRNA was fragmented by metal-induced hydrolysis to break down full-length cRNA to 35-200 base fragments.  15ug of adjusted cRNA was used to prepare 300ul of hybridization cocktail of which 200ul was hybridized with the GeneChip.   ; Protocol Type = labeling; Performers: John,Y,Okyere, Janet,,Higgins

growth_protocol: unknown; Parameter location = Growth Room; Parameter Temperature = 22oC; Parameter Lighting = 24h light; Parameter Medium = compost; Parameter Water = twice a week; Parameter Stratification = 2 days at 4oC; Performer: Christian,,Dubos

extract_protocol: Qiagen / Trizol; Protocol: Qiagen RNAeasy kit protocol ; Protocol Type = nucleic_acid_extraction; Performer: Christian,,Dubos

tag: OrganismPart

value: cork

tag: Ecotype


title: MC002_ATH1_A2.1-dubos-wtc

institute: Nottingham Arabidopsis Stock Centre (NASC)

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 75.30
Background Stdev (float) = 2.08
Background Max (float) = 81.6
Background Min (float) = 71.8
Noise Avg (float) = 3.88
Noise Stdev (float) = 0.22
Noise Max (float) = 4.7
Noise Min (float) = 3.5
RawQ (float) = 2.85
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 416
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: MC002_ATH1_A2.1-dubos-wtc.DAT

hyb_protocol: EukGE-WS2v4; Protocol Type = Hybridization; Parameter Wash A1 Recovery Mixes = 0 (type integer); Parameter Wash A1 Temperature (C) = 25 (type integer); Parameter Number of Wash A1 Cycles = 10 (type integer); Parameter Mixes per Wash A1 Cycle = 2 (type integer); Parameter Wash B Recovery Mixes = 0 (type integer); Parameter Wash B Temperature (C) = 50 (type integer); Parameter Number of Wash B Cycles = 4 (type integer); Parameter Mixes per Wash B Cycle = 15 (type integer); Parameter Stain Temperature (C) = 25 (type integer); Parameter First Stain Time (seconds) = 600 (type integer); Parameter Wash A2 Recovery Mixes = 0 (type integer); Parameter Wash A2 Temperature (C) = 25 (type integer); Parameter Number of Wash A2 Cycles = 10 (type integer); Parameter Mixes per Wash A2 Cycle = 4 (type integer); Parameter Second Stain Time (seconds) = 600 (type integer); Parameter Third Stain Time (seconds) = 600 (type integer); Parameter Wash A3 Recovery Mixes = 0 (type integer); Parameter Wash A3 Temperature (C) = 30 (type integer); Parameter Number of Wash A3 Cycles = 15 (type integer); Parameter Mixes per Wash A3 Cycle = 4 (type integer); Parameter Holding Temperature (C) = 25 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Fluidics Station, Parameter: Parameter Station Number = 1 (type integer), Parameter: Parameter Position = 2 (type integer); Performers: John,Y,Okyere, , Janet,,Higgins

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 237 (type integer); Parameter Upper left corner y coordinate = 225 (type integer); Parameter Upper right corner x coordinate = 4505 (type integer); Parameter Upper right corner y coordinate = 138 (type integer); Parameter Lower right corner x coordinate = 4593 (type integer); Parameter Lower right corner y coordinate = 4408 (type integer); Parameter Lower left corner x coordinate = 326 (type integer); Parameter Lower left corner y coordinate = 4495 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 0.397080 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;


title: MC002_ATH1_A1.3-dubos-wtx

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 123.77
Background Stdev (float) = 3.49
Background Max (float) = 134.9
Background Min (float) = 118.3
Noise Avg (float) = 6.82
Noise Stdev (float) = 0.44
Noise Max (float) = 8.1
Noise Min (float) = 6.0
RawQ (float) = 4.43
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 418
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: MC002_ATH1_A1.3-dubos-wtx.DAT

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 231 (type integer); Parameter Upper left corner y coordinate = 232 (type integer); Parameter Upper right corner x coordinate = 4501 (type integer); Parameter Upper right corner y coordinate = 242 (type integer); Parameter Lower right corner x coordinate = 4492 (type integer); Parameter Lower right corner y coordinate = 4513 (type integer); Parameter Lower left corner x coordinate = 222 (type integer); Parameter Lower left corner y coordinate = 4502 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 0.206862 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;

value: xylem


title: DV001_ATH1_A7-degra-Ec1

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 84.70
Background Stdev (float) = 1.31
Background Max (float) = 89.5
Background Min (float) = 81.1
Noise Avg (float) = 5.68
Noise Stdev (float) = 0.28
Noise Max (float) = 6.3
Noise Min (float) = 4.8
RawQ (float) = 3.30
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 377
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: DV001_ATH1_A7-degra-Ec1.DAT

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 230 (type integer); Parameter Upper left corner y coordinate = 232 (type integer); Parameter Upper right corner x coordinate = 4497 (type integer); Parameter Upper right corner y coordinate = 258 (type integer); Parameter Lower right corner x coordinate = 4472 (type integer); Parameter Lower right corner y coordinate = 4528 (type integer); Parameter Lower left corner x coordinate = 206 (type integer); Parameter Lower left corner y coordinate = 4502 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 0.310633 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;

source_name: De Grauwe_1-7_water-etr1-1hr_Rep3_ATH1

growth_protocol: unknown; Parameter location = Growth Room; Parameter Atmosphere = Normal; Parameter Humidity = 70% RH; Parameter Temperature = 22 C; Parameter Lighting = 16/8; Parameter Medium = MS/2; Parameter Nutrients = 1%sucrose; Performer: Liesbeth,,De Grauwe

extract_protocol: Qiagen / Trizol; Protocol: Qiagen RNAeasy kit protocol ; Protocol Type = nucleic_acid_extraction; Performer: Liesbeth,,De Grauwe

treatment_protocol: This plant was sprayed with water, and harvested 1hour after treatment ; Performer: Liesbeth,,De Grauwe

value: Whole plant

value: Col

tag: NASC:Growth Stage

tag: StrainOrLine


title: MC002_ATH1_A1.2-dubos-wtx

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 94.76
Background Stdev (float) = 2.01
Background Max (float) = 101.0
Background Min (float) = 90.7
Noise Avg (float) = 5.11
Noise Stdev (float) = 0.23
Noise Max (float) = 6.0
Noise Min (float) = 4.6
RawQ (float) = 3.53
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 309
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: MC002_ATH1_A1.2-dubos-wtx.DAT

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 231 (type integer); Parameter Upper left corner y coordinate = 230 (type integer); Parameter Upper right corner x coordinate = 4501 (type integer); Parameter Upper right corner y coordinate = 223 (type integer); Parameter Lower right corner x coordinate = 4510 (type integer); Parameter Lower right corner y coordinate = 4493 (type integer); Parameter Lower left corner x coordinate = 240 (type integer); Parameter Lower left corner y coordinate = 4500 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 0.254352 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;


title: DV002_ATH1_A9-degra-Eg1a

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 87.91
Background Stdev (float) = 3.65
Background Max (float) = 98.2
Background Min (float) = 81.4
Noise Avg (float) = 5.02
Noise Stdev (float) = 0.36
Noise Max (float) = 6.2
Noise Min (float) = 4.4
RawQ (float) = 3.47
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 219
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: DV002_ATH1_A9-degra-Eg1a.DAT

hyb_protocol: EukGE-WS2v4; Protocol Type = Hybridization; Parameter Wash A1 Recovery Mixes = 0 (type integer); Parameter Wash A1 Temperature (C) = 25 (type integer); Parameter Number of Wash A1 Cycles = 10 (type integer); Parameter Mixes per Wash A1 Cycle = 2 (type integer); Parameter Wash B Recovery Mixes = 0 (type integer); Parameter Wash B Temperature (C) = 50 (type integer); Parameter Number of Wash B Cycles = 4 (type integer); Parameter Mixes per Wash B Cycle = 15 (type integer); Parameter Stain Temperature (C) = 25 (type integer); Parameter First Stain Time (seconds) = 600 (type integer); Parameter Wash A2 Recovery Mixes = 0 (type integer); Parameter Wash A2 Temperature (C) = 25 (type integer); Parameter Number of Wash A2 Cycles = 10 (type integer); Parameter Mixes per Wash A2 Cycle = 4 (type integer); Parameter Second Stain Time (seconds) = 600 (type integer); Parameter Third Stain Time (seconds) = 600 (type integer); Parameter Wash A3 Recovery Mixes = 0 (type integer); Parameter Wash A3 Temperature (C) = 30 (type integer); Parameter Number of Wash A3 Cycles = 15 (type integer); Parameter Mixes per Wash A3 Cycle = 4 (type integer); Parameter Holding Temperature (C) = 25 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Fluidics Station, Parameter: Parameter Station Number = 1 (type integer), Parameter: Parameter Position = 4 (type integer); Performers: John,Y,Okyere, , Janet,,Higgins

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 227 (type integer); Parameter Upper left corner y coordinate = 233 (type integer); Parameter Upper right corner x coordinate = 4498 (type integer); Parameter Upper right corner y coordinate = 285 (type integer); Parameter Lower right corner x coordinate = 4449 (type integer); Parameter Lower right corner y coordinate = 4555 (type integer); Parameter Lower left corner x coordinate = 177 (type integer); Parameter Lower left corner y coordinate = 4503 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 0.351393 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;

source_name: De Grauwe_1-9_GA4-etr1-1hr_Rep1_ATH1

treatment_protocol: This plant was sprayed with GA4, which was dissolved in water, and harvested 1hour after treatment. ; Performer: Liesbeth,,De Grauwe


title: DV002_ATH1_A7-degra-Ec1b

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 85.43
Background Stdev (float) = 2.78
Background Max (float) = 91.9
Background Min (float) = 79.2
Noise Avg (float) = 4.71
Noise Stdev (float) = 0.20
Noise Max (float) = 5.3
Noise Min (float) = 4.4
RawQ (float) = 3.17
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 275
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: DV002_ATH1_A7-degra-Ec1b.DAT

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 227 (type integer); Parameter Upper left corner y coordinate = 233 (type integer); Parameter Upper right corner x coordinate = 4498 (type integer); Parameter Upper right corner y coordinate = 281 (type integer); Parameter Lower right corner x coordinate = 4451 (type integer); Parameter Lower right corner y coordinate = 4551 (type integer); Parameter Lower left corner x coordinate = 180 (type integer); Parameter Lower left corner y coordinate = 4503 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 0.422143 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;

source_name: De Grauwe_1-7_water-etr1-1hr_Rep2_ATH1


title: MC002_ATH1_A1.1-dubos-wtx

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 85.37
Background Stdev (float) = 1.68
Background Max (float) = 91.7
Background Min (float) = 82.6
Noise Avg (float) = 4.71
Noise Stdev (float) = 0.26
Noise Max (float) = 5.8
Noise Min (float) = 4.3
RawQ (float) = 3.38
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 688
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: MC002_ATH1_A1.1-dubos-wtx.DAT

hyb_protocol: EukGE-WS2v4; Protocol Type = Hybridization; Parameter Wash A1 Recovery Mixes = 0 (type integer); Parameter Wash A1 Temperature (C) = 25 (type integer); Parameter Number of Wash A1 Cycles = 10 (type integer); Parameter Mixes per Wash A1 Cycle = 2 (type integer); Parameter Wash B Recovery Mixes = 0 (type integer); Parameter Wash B Temperature (C) = 50 (type integer); Parameter Number of Wash B Cycles = 4 (type integer); Parameter Mixes per Wash B Cycle = 15 (type integer); Parameter Stain Temperature (C) = 25 (type integer); Parameter First Stain Time (seconds) = 600 (type integer); Parameter Wash A2 Recovery Mixes = 0 (type integer); Parameter Wash A2 Temperature (C) = 25 (type integer); Parameter Number of Wash A2 Cycles = 10 (type integer); Parameter Mixes per Wash A2 Cycle = 4 (type integer); Parameter Second Stain Time (seconds) = 600 (type integer); Parameter Third Stain Time (seconds) = 600 (type integer); Parameter Wash A3 Recovery Mixes = 0 (type integer); Parameter Wash A3 Temperature (C) = 30 (type integer); Parameter Number of Wash A3 Cycles = 15 (type integer); Parameter Mixes per Wash A3 Cycle = 4 (type integer); Parameter Holding Temperature (C) = 25 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Fluidics Station, Parameter: Parameter Station Number = 1 (type integer), Parameter: Parameter Position = 1 (type integer); Performers: John,Y,Okyere, , Janet,,Higgins

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 233 (type integer); Parameter Upper left corner y coordinate = 229 (type integer); Parameter Upper right corner x coordinate = 4502 (type integer); Parameter Upper right corner y coordinate = 216 (type integer); Parameter Lower right corner x coordinate = 4517 (type integer); Parameter Lower right corner y coordinate = 4486 (type integer); Parameter Lower left corner x coordinate = 247 (type integer); Parameter Lower left corner y coordinate = 4499 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 0.296556 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;


title: DV002_ATH1_A2-degra-Cc1a

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 79.51
Background Stdev (float) = 2.54
Background Max (float) = 85.3
Background Min (float) = 73.7
Noise Avg (float) = 5.07
Noise Stdev (float) = 0.17
Noise Max (float) = 5.4
Noise Min (float) = 4.6
RawQ (float) = 2.86
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 528
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: DV002_ATH1_A2-degra-Cc1a.DAT

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 234 (type integer); Parameter Upper left corner y coordinate = 229 (type integer); Parameter Upper right corner x coordinate = 4504 (type integer); Parameter Upper right corner y coordinate = 197 (type integer); Parameter Lower right corner x coordinate = 4539 (type integer); Parameter Lower right corner y coordinate = 4467 (type integer); Parameter Lower left corner x coordinate = 269 (type integer); Parameter Lower left corner y coordinate = 4499 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 0.257493 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;

source_name: De Grauwe_1-2_water-Col0-1hr_Rep1_ATH1

treatment_protocol: This plant was sprayed with water and harvested 1hour after treatment. ; Performer: Liesbeth,,De Grauwe


title: DV002_ATH1_A4-degra-Cg1a

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 85.37
Background Stdev (float) = 1.95
Background Max (float) = 90.7
Background Min (float) = 80.6
Noise Avg (float) = 5.51
Noise Stdev (float) = 0.21
Noise Max (float) = 6.1
Noise Min (float) = 5.0
RawQ (float) = 3.08
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 819
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: DV002_ATH1_A4-degra-Cg1a.DAT

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 229 (type integer); Parameter Upper left corner y coordinate = 233 (type integer); Parameter Upper right corner x coordinate = 4499 (type integer); Parameter Upper right corner y coordinate = 261 (type integer); Parameter Lower right corner x coordinate = 4471 (type integer); Parameter Lower right corner y coordinate = 4531 (type integer); Parameter Lower left corner x coordinate = 201 (type integer); Parameter Lower left corner y coordinate = 4502 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 0.281759 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;

source_name: De Grauwe_1-4_GA4-Col0-1hr_Rep1_ATH1

treatment_protocol: This plant was sprayed GA4, which was dissolved in water, and harvested 1hour after treatment. ; Performer: Liesbeth,,De Grauwe


title: DV002_ATH1_A2-degra-Cc1b

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 84.71
Background Stdev (float) = 1.30
Background Max (float) = 88.2
Background Min (float) = 81.9
Noise Avg (float) = 5.18
Noise Stdev (float) = 0.21
Noise Max (float) = 6.0
Noise Min (float) = 4.5
RawQ (float) = 3.09
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 625
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: DV002_ATH1_A2-degra-Cc1b.DAT

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 227 (type integer); Parameter Upper left corner y coordinate = 233 (type integer); Parameter Upper right corner x coordinate = 4496 (type integer); Parameter Upper right corner y coordinate = 284 (type integer); Parameter Lower right corner x coordinate = 4446 (type integer); Parameter Lower right corner y coordinate = 4553 (type integer); Parameter Lower left corner x coordinate = 177 (type integer); Parameter Lower left corner y coordinate = 4503 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 0.356169 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;

source_name: De Grauwe_1-2_water-Col0-1hr_Rep2_ATH1

treatment_protocol: This plant was sprayed with water and harvested 1hour after treatment ; Performer: Liesbeth,,De Grauwe


title: BD001_ATH1_A6-DAVIE-T14

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 85.12
Background Stdev (float) = 1.71
Background Max (float) = 89.0
Background Min (float) = 80.0
Noise Avg (float) = 4.51
Noise Stdev (float) = 0.15
Noise Max (float) = 4.9
Noise Min (float) = 4.2
RawQ (float) = 3.15
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 1305
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: BD001_ATH1_A6-DAVIE-T14.DAT

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 229 (type integer); Parameter Upper left corner y coordinate = 234 (type integer); Parameter Upper right corner x coordinate = 4496 (type integer); Parameter Upper right corner y coordinate = 277 (type integer); Parameter Lower right corner x coordinate = 4453 (type integer); Parameter Lower right corner y coordinate = 4548 (type integer); Parameter Lower left corner x coordinate = 186 (type integer); Parameter Lower left corner y coordinate = 4504 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 0.724345 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;

growth_protocol: unknown; Parameter location = Greenhouse; Parameter Temperature = 18; Parameter Lighting = 16 hour days; Parameter Medium = compost; Parameter Water = daily at 10 am; Performer: Brendan,,Davies

extract_protocol: Qiagen / Trizol; Protocol: Qiagen RNAeasy kit protocol ; Protocol Type = nucleic_acid_extraction; Performer: Brendan,,Davies

value: inflorescence

value: ws

tag: GeneticModification

value: TDNA insert into At1g69690


title: DV002_ATH1_A4-degra-Cg1b

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 84.28
Background Stdev (float) = 1.53
Background Max (float) = 87.3
Background Min (float) = 79.5
Noise Avg (float) = 5.31
Noise Stdev (float) = 0.19
Noise Max (float) = 5.8
Noise Min (float) = 4.9
RawQ (float) = 3.16
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 316
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: DV002_ATH1_A4-degra-Cg1b.DAT

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 228 (type integer); Parameter Upper left corner y coordinate = 233 (type integer); Parameter Upper right corner x coordinate = 4498 (type integer); Parameter Upper right corner y coordinate = 271 (type integer); Parameter Lower right corner x coordinate = 4460 (type integer); Parameter Lower right corner y coordinate = 4541 (type integer); Parameter Lower left corner x coordinate = 190 (type integer); Parameter Lower left corner y coordinate = 4503 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 0.302627 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;

source_name: De Grauwe_1-4_GA4-Col0-1hr_Rep2_ATH1

treatment_protocol: This plant was sprayed GA4, which was dissolved in water, and harvested 1hour after treatment ; Performer: Liesbeth,,De Grauwe


title: DV001_ATH1_A6-degra-Ec0_repeat2

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 118.23
Background Stdev (float) = 5.90
Background Max (float) = 134.4
Background Min (float) = 109.9
Noise Avg (float) = 6.78
Noise Stdev (float) = 0.55
Noise Max (float) = 8.2
Noise Min (float) = 5.8
RawQ (float) = 4.28
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 695
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: DV001_ATH1_A6-degra-Ec0_repeat2.DAT

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 235 (type integer); Parameter Upper left corner y coordinate = 228 (type integer); Parameter Upper right corner x coordinate = 4502 (type integer); Parameter Upper right corner y coordinate = 185 (type integer); Parameter Lower right corner x coordinate = 4546 (type integer); Parameter Lower right corner y coordinate = 4455 (type integer); Parameter Lower left corner x coordinate = 279 (type integer); Parameter Lower left corner y coordinate = 4498 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 0.323629 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;

source_name: De Grauwe_1-6_water-etr1-0hr_Rep1_ATH1

treatment_protocol: This plant was sprayed with water, and immediately harvested. ; Performer: Liesbeth,,De Grauwe


title: DV001_ATH1_A10-degra-Eg3_repeat2

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 100.09
Background Stdev (float) = 3.91
Background Max (float) = 113.5
Background Min (float) = 94.1
Noise Avg (float) = 6.70
Noise Stdev (float) = 0.53
Noise Max (float) = 8.4
Noise Min (float) = 5.8
RawQ (float) = 3.81
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 381
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: DV001_ATH1_A10-degra-Eg3_repeat2.DAT

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 234 (type integer); Parameter Upper left corner y coordinate = 229 (type integer); Parameter Upper right corner x coordinate = 4501 (type integer); Parameter Upper right corner y coordinate = 200 (type integer); Parameter Lower right corner x coordinate = 4532 (type integer); Parameter Lower right corner y coordinate = 4470 (type integer); Parameter Lower left corner x coordinate = 265 (type integer); Parameter Lower left corner y coordinate = 4499 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 0.427455 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;

source_name: De Grauwe_1-10_GA4-etr1-3hr_Rep1_ATH1

treatment_protocol: This plant was sprayed with GA4, which was dissolved in water, and harvested 3hours after treatment ; Performer: Liesbeth,,De Grauwe


title: DV001_ATH1_A1-degra-Cc0_repeat

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 101.23
Background Stdev (float) = 2.70
Background Max (float) = 110.9
Background Min (float) = 97.2
Noise Avg (float) = 5.73
Noise Stdev (float) = 0.31
Noise Max (float) = 6.6
Noise Min (float) = 5.1
RawQ (float) = 3.70
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 294
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: DV001_ATH1_A1-degra-Cc0_repeat.DAT

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 227 (type integer); Parameter Upper left corner y coordinate = 233 (type integer); Parameter Upper right corner x coordinate = 4494 (type integer); Parameter Upper right corner y coordinate = 272 (type integer); Parameter Lower right corner x coordinate = 4456 (type integer); Parameter Lower right corner y coordinate = 4542 (type integer); Parameter Lower left corner x coordinate = 189 (type integer); Parameter Lower left corner y coordinate = 4503 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 0.307355 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;

source_name: De Grauwe_1-1_water-Col0-0hr_Rep1_ATH1

treatment_protocol: This plant was sprayed with water and immediately harvested. ; Performer: Liesbeth,,De Grauwe


title: BD001_ATH1_A5-DAVIE-T14

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 84.90
Background Stdev (float) = 1.40
Background Max (float) = 87.9
Background Min (float) = 81.0
Noise Avg (float) = 4.26
Noise Stdev (float) = 0.16
Noise Max (float) = 4.8
Noise Min (float) = 3.9
RawQ (float) = 3.20
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 347
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: BD001_ATH1_A5-DAVIE-T14.DAT

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 230 (type integer); Parameter Upper left corner y coordinate = 231 (type integer); Parameter Upper right corner x coordinate = 4498 (type integer); Parameter Upper right corner y coordinate = 257 (type integer); Parameter Lower right corner x coordinate = 4474 (type integer); Parameter Lower right corner y coordinate = 4526 (type integer); Parameter Lower left corner x coordinate = 207 (type integer); Parameter Lower left corner y coordinate = 4502 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 1.060937 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;


title: DV001_ATH1_A4-degra-Cg1_repeat2

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 106.30
Background Stdev (float) = 2.70
Background Max (float) = 115.5
Background Min (float) = 102.2
Noise Avg (float) = 6.14
Noise Stdev (float) = 0.29
Noise Max (float) = 6.7
Noise Min (float) = 5.2
RawQ (float) = 3.94
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 459
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: DV001_ATH1_A4-degra-Cg1_repeat2.DAT

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 231 (type integer); Parameter Upper left corner y coordinate = 232 (type integer); Parameter Upper right corner x coordinate = 4498 (type integer); Parameter Upper right corner y coordinate = 248 (type integer); Parameter Lower right corner x coordinate = 4482 (type integer); Parameter Lower right corner y coordinate = 4518 (type integer); Parameter Lower left corner x coordinate = 216 (type integer); Parameter Lower left corner y coordinate = 4501 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 0.333784 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;

source_name: De Grauwe_1-4_GA4-Col0-1hr_Rep3_ATH1


title: DV001_ATH1_A9-degra-Eg1_repeat2

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 109.95
Background Stdev (float) = 4.21
Background Max (float) = 123.2
Background Min (float) = 102.1
Noise Avg (float) = 5.96
Noise Stdev (float) = 0.32
Noise Max (float) = 7.3
Noise Min (float) = 5.3
RawQ (float) = 4.02
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 681
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: DV001_ATH1_A9-degra-Eg1_repeat2.DAT

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 234 (type integer); Parameter Upper left corner y coordinate = 229 (type integer); Parameter Upper right corner x coordinate = 4501 (type integer); Parameter Upper right corner y coordinate = 206 (type integer); Parameter Lower right corner x coordinate = 4525 (type integer); Parameter Lower right corner y coordinate = 4477 (type integer); Parameter Lower left corner x coordinate = 258 (type integer); Parameter Lower left corner y coordinate = 4500 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 0.435900 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;

source_name: De Grauwe_1-9_GA4-etr1-1hr_Rep3_ATH1

treatment_protocol: This plant was sprayed with GA4, which was dissolved in water, and harvested 1hour after treatment ; Performer: Liesbeth,,De Grauwe


title: DV002_ATH1_A9-degra-Eg1b

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 93.63
Background Stdev (float) = 2.53
Background Max (float) = 100.5
Background Min (float) = 87.3
Noise Avg (float) = 5.38
Noise Stdev (float) = 0.33
Noise Max (float) = 6.6
Noise Min (float) = 4.5
RawQ (float) = 3.49
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 177
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: DV002_ATH1_A9-degra-Eg1b.DAT

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 230 (type integer); Parameter Upper left corner y coordinate = 232 (type integer); Parameter Upper right corner x coordinate = 4501 (type integer); Parameter Upper right corner y coordinate = 245 (type integer); Parameter Lower right corner x coordinate = 4489 (type integer); Parameter Lower right corner y coordinate = 4515 (type integer); Parameter Lower left corner x coordinate = 218 (type integer); Parameter Lower left corner y coordinate = 4502 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 0.452886 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;

source_name: De Grauwe_1-9_GA4-etr1-1hr_Rep2_ATH1


title: BD001_ATH1_A4-DAVIE-T13

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 111.86
Background Stdev (float) = 3.81
Background Max (float) = 123.1
Background Min (float) = 105.1
Noise Avg (float) = 7.37
Noise Stdev (float) = 0.31
Noise Max (float) = 8.3
Noise Min (float) = 6.4
RawQ (float) = 4.21
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 695
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: BD001_ATH1_A4-DAVIE-T13.DAT

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 230 (type integer); Parameter Upper left corner y coordinate = 233 (type integer); Parameter Upper right corner x coordinate = 4497 (type integer); Parameter Upper right corner y coordinate = 268 (type integer); Parameter Lower right corner x coordinate = 4463 (type integer); Parameter Lower right corner y coordinate = 4539 (type integer); Parameter Lower left corner x coordinate = 196 (type integer); Parameter Lower left corner y coordinate = 4503 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 0.590528 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;

value: TDNA insert into At3g47620


title: DV001_ATH1_A3-degra-Cgh_repeat2

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 96.82
Background Stdev (float) = 0.90
Background Max (float) = 99.7
Background Min (float) = 93.3
Noise Avg (float) = 5.73
Noise Stdev (float) = 0.21
Noise Max (float) = 6.3
Noise Min (float) = 5.2
RawQ (float) = 3.69
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 515
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: DV001_ATH1_A3-degra-Cgh_repeat2.DAT

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 231 (type integer); Parameter Upper left corner y coordinate = 232 (type integer); Parameter Upper right corner x coordinate = 4498 (type integer); Parameter Upper right corner y coordinate = 251 (type integer); Parameter Lower right corner x coordinate = 4481 (type integer); Parameter Lower right corner y coordinate = 4521 (type integer); Parameter Lower left corner x coordinate = 214 (type integer); Parameter Lower left corner y coordinate = 4502 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 0.314975 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;

source_name: De Grauwe_1-3_GA4-Col0-30min_Rep1_ATH1

treatment_protocol: This plant was sprayed GA4, which was dissolved in water, and harvested 30min after treatment. ; Performer: Liesbeth,,De Grauwe


title: BD001_ATH1_A3-DAVIE-T13

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 98.49
Background Stdev (float) = 1.04
Background Max (float) = 101.4
Background Min (float) = 95.7
Noise Avg (float) = 4.71
Noise Stdev (float) = 0.19
Noise Max (float) = 5.3
Noise Min (float) = 4.2
RawQ (float) = 3.56
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 212
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: BD001_ATH1_A3-DAVIE-T13.DAT

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 230 (type integer); Parameter Upper left corner y coordinate = 233 (type integer); Parameter Upper right corner x coordinate = 4497 (type integer); Parameter Upper right corner y coordinate = 271 (type integer); Parameter Lower right corner x coordinate = 4460 (type integer); Parameter Lower right corner y coordinate = 4541 (type integer); Parameter Lower left corner x coordinate = 193 (type integer); Parameter Lower left corner y coordinate = 4503 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 0.968745 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;


title: BD001_ATH1_A1-DAVIE-CON

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 112.87
Background Stdev (float) = 4.34
Background Max (float) = 127.1
Background Min (float) = 105.9
Noise Avg (float) = 6.15
Noise Stdev (float) = 0.52
Noise Max (float) = 8.0
Noise Min (float) = 5.3
RawQ (float) = 4.11
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 1014
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: BD001_ATH1_A1-DAVIE-CON.DAT

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 230 (type integer); Parameter Upper left corner y coordinate = 231 (type integer); Parameter Upper right corner x coordinate = 4497 (type integer); Parameter Upper right corner y coordinate = 249 (type integer); Parameter Lower right corner x coordinate = 4482 (type integer); Parameter Lower right corner y coordinate = 4519 (type integer); Parameter Lower left corner x coordinate = 214 (type integer); Parameter Lower left corner y coordinate = 4502 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 0.688871 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;


title: BD001_ATH1_A7-DAVIE-HET

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 86.81
Background Stdev (float) = 1.81
Background Max (float) = 91.6
Background Min (float) = 82.9
Noise Avg (float) = 4.73
Noise Stdev (float) = 0.15
Noise Max (float) = 5.1
Noise Min (float) = 4.2
RawQ (float) = 3.20
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 223
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: BD001_ATH1_A7-DAVIE-HET.DAT

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 228 (type integer); Parameter Upper left corner y coordinate = 233 (type integer); Parameter Upper right corner x coordinate = 4496 (type integer); Parameter Upper right corner y coordinate = 273 (type integer); Parameter Lower right corner x coordinate = 4457 (type integer); Parameter Lower right corner y coordinate = 4543 (type integer); Parameter Lower left corner x coordinate = 189 (type integer); Parameter Lower left corner y coordinate = 4503 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 0.559454 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;

value: heterozygous for an insertion at At1g69690 and an insertion at At3g47620

value: HET


title: DV002_ATH1_A7-degra-Ec1a

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 86.07
Background Stdev (float) = 1.99
Background Max (float) = 91.7
Background Min (float) = 80.6
Noise Avg (float) = 5.17
Noise Stdev (float) = 0.21
Noise Max (float) = 6.0
Noise Min (float) = 4.7
RawQ (float) = 3.43
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 510
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: DV002_ATH1_A7-degra-Ec1a.DAT

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 230 (type integer); Parameter Upper left corner y coordinate = 231 (type integer); Parameter Upper right corner x coordinate = 4502 (type integer); Parameter Upper right corner y coordinate = 241 (type integer); Parameter Lower right corner x coordinate = 4493 (type integer); Parameter Lower right corner y coordinate = 4511 (type integer); Parameter Lower left corner x coordinate = 222 (type integer); Parameter Lower left corner y coordinate = 4501 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 0.404532 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;

source_name: De Grauwe_1-7_water-etr1-1hr_Rep1_ATH1

treatment_protocol: This plant was sprayed with water, and harvested 1hour after treatment. ; Performer: Liesbeth,,De Grauwe


title: BD001_ATH1_A2-DAVIE-CON

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 87.36
Background Stdev (float) = 1.59
Background Max (float) = 92.2
Background Min (float) = 83.9
Noise Avg (float) = 5.29
Noise Stdev (float) = 0.13
Noise Max (float) = 5.8
Noise Min (float) = 4.9
RawQ (float) = 3.28
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 404
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: BD001_ATH1_A2-DAVIE-CON.DAT

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 228 (type integer); Parameter Upper left corner y coordinate = 234 (type integer); Parameter Upper right corner x coordinate = 4495 (type integer); Parameter Upper right corner y coordinate = 289 (type integer); Parameter Lower right corner x coordinate = 4442 (type integer); Parameter Lower right corner y coordinate = 4559 (type integer); Parameter Lower left corner x coordinate = 174 (type integer); Parameter Lower left corner y coordinate = 4504 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 0.354284 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;


title: DB001_ATH1_A6-Brown-cal

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 82.04
Background Stdev (float) = 2.17
Background Max (float) = 88.2
Background Min (float) = 76.7
Noise Avg (float) = 4.88
Noise Stdev (float) = 0.16
Noise Max (float) = 5.3
Noise Min (float) = 4.4
RawQ (float) = 2.99
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 192
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: DB001_ATH1_A6-Brown-cal.DAT

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 230 (type integer); Parameter Upper left corner y coordinate = 232 (type integer); Parameter Upper right corner x coordinate = 4498 (type integer); Parameter Upper right corner y coordinate = 260 (type integer); Parameter Lower right corner x coordinate = 4472 (type integer); Parameter Lower right corner y coordinate = 4530 (type integer); Parameter Lower left corner x coordinate = 204 (type integer); Parameter Lower left corner y coordinate = 4502 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 0.336009 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;

growth_protocol: unknown; Parameter location = Growth Room; Parameter Temperature = 22oC; Parameter Medium = Murahige and Skoog; Parameter Treatment = 0.2mgl-1 auxin( 2,4-D NA IAA) 1.0mgl-1 BA; Parameter Nutrients = 0.2% sucrose 4% mannitol; Performer: David,,Brown

extract_protocol: Qiagen Plant RNAeasy kit; Protocol: Seedlings harvested and snap-frozen in liquid nitrogen and RNA extracted as described in the Qiagen RNAeasy kit. ; Protocol Type = nucleic_acid_extraction; Performer: David,,Brown

treatment_protocol: Leaves from seedlings, grown aseptically (14-17 days)on MS agar, excised and transferred to callus induction medium (Gamborg's B5 Basil Medium with minimal organics, 2.0% glucose, 0.5mgl-1 MES, 0.5mgl-1 2,4-D, 0.05mgl-1 Kinetin. pH 5.7). Incubated in darkness, at 22oC for 2 weeks. Callus transferred to low differentiating liquid media (MS 0.2% sucrose, 4% mannitol, 0.5mgl-1 2,4-D, 1.5ugl-1 Kinetin, pH5.7). Incubated at 22oC on a rotary shaker (105rpm). Sample taken at day =9 ; Performer: David,,Brown

value: Cell culture

tag: Age time point

value: 9 day timepoint

value: Ler


title: DB001_ATH1_A3-Brown-cal

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 78.05
Background Stdev (float) = 0.88
Background Max (float) = 80.8
Background Min (float) = 75.2
Noise Avg (float) = 5.09
Noise Stdev (float) = 0.19
Noise Max (float) = 5.5
Noise Min (float) = 4.3
RawQ (float) = 2.87
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 612
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: DB001_ATH1_A3-Brown-cal.DAT

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 230 (type integer); Parameter Upper left corner y coordinate = 233 (type integer); Parameter Upper right corner x coordinate = 4497 (type integer); Parameter Upper right corner y coordinate = 263 (type integer); Parameter Lower right corner x coordinate = 4468 (type integer); Parameter Lower right corner y coordinate = 4533 (type integer); Parameter Lower left corner x coordinate = 200 (type integer); Parameter Lower left corner y coordinate = 4503 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 0.624993 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;

treatment_protocol: Leaves from seedlings, grown aseptically (14-17 days) on MS agar, excised and transferred to callus induction medium (Gamborg's B5 Basil Medium with minimal organics, 2.0% glucose, 0.5mgl-1 MES, 0.5mgl-1 2,4-D, 0.05mgl-1 Kinetin. pH 5.7). Incubated in darkness, at 22oC for 2 weeks. Callus transferred to high differentiating liquid media (MS 0.2% sucrose, 4% mannitol, 0.2mgl-1 auxins (2,4-D, NAA, IAA) 1mgl-1 BA, pH5.7). Incubated at 22oC on a rotary shaker (105rpm). Sample taken at day =5 ; Performer: David,,Brown

value: 5 day timepoint


title: BD001_ATH1_A8-Davie-HET_repeat

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 83.50
Background Stdev (float) = 1.50
Background Max (float) = 87.7
Background Min (float) = 79.7
Noise Avg (float) = 4.81
Noise Stdev (float) = 0.16
Noise Max (float) = 5.3
Noise Min (float) = 4.3
RawQ (float) = 3.35
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 335
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: BD001_ATH1_A8-Davie-HET_repeat.DAT

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 228 (type integer); Parameter Upper left corner y coordinate = 233 (type integer); Parameter Upper right corner x coordinate = 4496 (type integer); Parameter Upper right corner y coordinate = 288 (type integer); Parameter Lower right corner x coordinate = 4443 (type integer); Parameter Lower right corner y coordinate = 4558 (type integer); Parameter Lower left corner x coordinate = 174 (type integer); Parameter Lower left corner y coordinate = 4503 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 0.704091 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;


title: DB001_ATH1_A4-Brown-cal

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 74.33
Background Stdev (float) = 1.92
Background Max (float) = 78.4
Background Min (float) = 69.0
Noise Avg (float) = 4.43
Noise Stdev (float) = 0.14
Noise Max (float) = 5.0
Noise Min (float) = 4.0
RawQ (float) = 2.84
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 254
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: DB001_ATH1_A4-Brown-cal.DAT

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 230 (type integer); Parameter Upper left corner y coordinate = 232 (type integer); Parameter Upper right corner x coordinate = 4498 (type integer); Parameter Upper right corner y coordinate = 259 (type integer); Parameter Lower right corner x coordinate = 4472 (type integer); Parameter Lower right corner y coordinate = 4530 (type integer); Parameter Lower left corner x coordinate = 203 (type integer); Parameter Lower left corner y coordinate = 4502 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 0.377090 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;

treatment_protocol: Leaves from seedlings, grown aseptically (14-17 days)on MS agar, excised and transferred to callus induction medium (Gamborg's B5 Basil Medium with minimal organics, 2.0% glucose, 0.5mgl-1 MES, 0.5mgl-1 2,4-D, 0.05mgl-1 Kinetin. pH 5.7). Incubated in darkness, at 22oC for 2 weeks. Callus transferred to high differentiating liquid media (MS 0.2% sucrose, 4% mannitol, 0.2mgl-1 auxins (2,4-D, NAA, IAA) 1mgl-1 BA, pH5.7). Incubated at 22oC on a rotary shaker (105rpm). Sample taken at day =9 ; Performer: David,,Brown


title: DB001_ATH1_A1-Brown-cal

description:
Rows (integer) = 712
Cols (integer) = 712
Number of Probe Sets (integer) = 22810
Background Avg (float) = 69.04
Background Stdev (float) = 1.36
Background Max (float) = 73.6
Background Min (float) = 65.1
Noise Avg (float) = 4.43
Noise Stdev (float) = 0.21
Noise Max (float) = 5.2
Noise Min (float) = 4.0
RawQ (float) = 2.70
Number Cells Masked (integer) = 0
Number Outlier Cells (integer) = 302
Number Cells Modified (integer) = 0
Rows (integer) = 712
Cols (integer) = 712
Number of Cells (integer) = 506944
Image: DB001_ATH1_A1-Brown-cal.DAT

scan_protocol: Protocol Type = CEL Analysis; Parameter Algorithm name = Percentile (type string); Parameter Upper left corner x coordinate = 232 (type integer); Parameter Upper left corner y coordinate = 230 (type integer); Parameter Upper right corner x coordinate = 4499 (type integer); Parameter Upper right corner y coordinate = 223 (type integer); Parameter Lower right corner x coordinate = 4508 (type integer); Parameter Lower right corner y coordinate = 4493 (type integer); Parameter Lower left corner x coordinate = 241 (type integer); Parameter Lower left corner y coordinate = 4500 (type integer); Parameter Percentile = 75 (type integer); Parameter Cell margin = 2 (type integer); Parameter Outlier high = 1.500 (type float); Parameter Outlier low = 1.004 (type float); Software: type: Acquisition and analysis software; Protocol Type = Scan; Parameter Pixel Size = 3 (type integer); Parameter Number of Scans = 2 (type integer); Software: type: Acquisition and analysis software; Hardware: type: Scanner, Parameter: Parameter Scanner ID = not specified (type string), Parameter: Parameter Scanner Type = HP (type string); Performers: John,Y,Okyere, , Janet,,Higgins

data_processing: Protocol Type = CHP Analysis; Parameter Algorithm name = ExpressionStat (type string); Parameter Algorithm version = 5.0 (type string); Parameter Baseline file = not specified (type string); Parameter Alpha1 = 0.04 (type float); Parameter Alpha2 = 0.06 (type float); Parameter Tau = 0.015 (type float); Parameter Gamma1H = 0.0025 (type float); Parameter Gamma1L = 0.0025 (type float); Parameter Gamma2H = 0.003 (type float); Parameter Gamma2L = 0.003 (type float); Parameter Perturbation = 1.1 (type float); Parameter Normalization factor = 1.000000 (type float); Parameter Scale factor = 0.314232 (type float); Parameter Mask file = not specified (type string); Software: type: Acquisition and analysis software;

treatment_protocol: Leaves from seedlings, grown aseptically (14-17 days) on MS agar, excised and transferred to callus induction medium (Gamborg's B5 Basil Medium with minimal organics, 2.0% glucose, 0.5mgl-1 MES, 0.5mgl-1 2,4-D, 0.05mgl-1 Kinetin. pH 5.7). Incubated in darkness, at 22oC for 2 weeks. Callus not transferred to liquid media. RNA collected therefore t=0. ; Performer: David,,Brown

value: 0 day timepoint