title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S168 [S168_V777L.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S143 [S143_S653C.Fulv+Palbo]

status: Public on Oct 19 2018

type: SRA

city: Cambridge

first: Ofir

last: Cohen

state: Massachusetts

address: 415 Main St, Cambridge, MA 02142

department: Cancer Program

country: USA

institute: Broad Institute

description: Parental cell line with HER2 S653C over-expression treated with 1µM Fulv + 10µM Palbo

library_source: transcriptomic

data_processing:
Library strategy: Smart-seq 2
mRNA-seq libraries were sequenced on an Illumina NextSeq 500 sequencer, V2 75 cycle kits
Reads were mapped with STAR aligner (Dobin et al. 2013)
Genome_build: hg19 of the human genome and build 12 of the GENCODE human genome annotation
Supplementary_files_format_and_content: rpkm and read counts

molecule: total RNA

organism: Homo sapiens

source_name: Breast Cancer

growth_protocol: T47D cells were infected de novo with lentivirus encoding HER2 mutants and controls (GFP, wild-type HER2, kinase-dead HER2, and ESR1 Y537S) in the pLX307 plasmid. Upon completion of selection with Puromycin, the cells were plated in 96-well plates in RPMI supplemented with 10% CSS for 48 hours.

extract_protocol: Plates with cell lysates were thawed and purified with 2.2x RNAClean SPRI beads (Beckman Coulter Genomics). The RNA captured beads were air-dried and processed immediately for RNA secondary structure denaturation (72˚C for three minutes) and cDNA synthesis. We performed SMART-Seq2 following the published protocol66

treatment_protocol: Cell were then treated with DMSO, 1μM fulvestrant, 1μM neratinib, 10μM palbociclib, 1μM fulvestrant + 1μM neratinib, or 1μM fulvestrant + 10μM palbociclib for 24 hours.

tag: cell line

tag: genotype/variation

value: HER2 S653C over-expression

tag: agent

value: 1microM Fulv + 10microM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S227 [S227_D845A.Fulv+Palbo]

description: Parental cell line with HER2 D845A over-expression treated with 1µM Fulv + 10µM Palbo

value: HER2 D845A over-expression


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S95 [S95_ESR1.Fulv+Palbo]

description: Parental cell line with ESR1 Y537S over-expression treated with 1µM Fulv + 10µM Palbo

value: ESR1 Y537S over-expression


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S239 [S239_S653C.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S251 [S251_L755S.Fulv+Palbo]

description: Parental cell line with HER2 L755S over-expression treated with 1µM Fulv + 10µM Palbo

value: HER2 L755S over-expression


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S47 [S47_S653C.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S72 [S72_V777L.Fulv+Palbo]

description: Parental cell line with HER2 V777L over-expression treated with 1µM Fulv + 10µM Palbo

value: HER2 V777L over-expression


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S11 [S11_GFP.Fulv+Palbo]

description: Parental cell line with GFP over-expression treated with 1µM Fulv + 10µM Palbo

value: GFP over-expression


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S155 [S155_L755S.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S70 [S70_V777L.Palbo]

description: Parental cell line with HER2 V777L over-expression treated with 10µM Palbo

value: 10microM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S23 [S23_wt.Fulv+Palbo]

description: Parental cell line with HER2 wt over-expression treated with 1µM Fulv + 10µM Palbo

value: HER2 wt over-expression


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S82 [S82_L869R.Palbo]

description: Parental cell line with HER2 L869R over-expression treated with 10µM Palbo

value: HER2 L869R over-expression


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S178 [S178_L869R.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S202 [S202_GFP.Palbo]

description: Parental cell line with GFP over-expression treated with 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S118 [S118_wt.Palbo]

description: Parental cell line with HER2 wt over-expression treated with 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S34 [S34_D845A.Palbo]

description: Parental cell line with HER2 D845A over-expression treated with 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S286 [S286_ESR1.Palbo]

description: Parental cell line with ESR1 Y537S over-expression treated with 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S154 [S154_L755S.Palbo]

description: Parental cell line with HER2 L755S over-expression treated with 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S214 [S214_wt.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S262 [S262_V777L.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S263 [S263_V777L.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S22 [S22_wt.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S46 [S46_S653C.Palbo]

description: Parental cell line with HER2 S653C over-expression treated with 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S10 [S10_GFP.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S274 [S274_L869R.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S106 [S106_GFP.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S142 [S142_S653C.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S215 [S215_wt.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S238 [S238_S653C.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S94 [S94_ESR1.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S166 [S166_V777L.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S226 [S226_D845A.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S250 [S250_L755S.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S58 [S58_L755S.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S130 [S130_D845A.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S190 [S190_ESR1.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S81 [S81_L869R.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S189 [S189_ESR1.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S141 [S141_S653C.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S201 [S201_GFP.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S93 [S93_ESR1.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S165 [S165_V777L.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S35 [S35_D845A.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S177 [S177_L869R.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S225 [S225_D845A.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S45 [S45_S653C.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S9 [S9_GFP.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S117 [S117_wt.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S261 [S261_V777L.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S237 [S237_S653C.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S213 [S213_wt.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S129 [S129_D845A.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S153 [S153_L755S.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S285 [S285_ESR1.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S273 [S273_L869R.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S21 [S21_wt.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S69 [S69_V777L.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S57 [S57_L755S.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S80 [S80_L869R.Fulv+Ner]

description: Parental cell line with HER2 L869R over-expression treated with 1µM Ful + 1µM Ner

value: 1microM Ful + 1microM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S44 [S44_S653C.Fulv+Ner]

description: Parental cell line with HER2 S653C over-expression treated with 1µM Ful + 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S20 [S20_wt.Fulv+Ner]

description: Parental cell line with HER2 wt over-expression treated with 1µM Ful + 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S200 [S200_GFP.Fulv+Ner]

description: Parental cell line with GFP over-expression treated with 1µM Ful + 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S33 [S33_D845A.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S164 [S164_V777L.Fulv+Ner]

description: Parental cell line with HER2 V777L over-expression treated with 1µM Ful + 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S56 [S56_L755S.Fulv+Ner]

description: Parental cell line with HER2 L755S over-expression treated with 1µM Ful + 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S188 [S188_ESR1.Fulv+Ner]

description: Parental cell line with ESR1 Y537S over-expression treated with 1µM Ful + 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S104 [S104_GFP.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S68 [S68_V777L.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S272 [S272_L869R.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S116 [S116_wt.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S105 [S105_GFP.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S92 [S92_ESR1.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S140 [S140_S653C.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S212 [S212_wt.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S224 [S224_D845A.Fulv+Ner]

description: Parental cell line with HER2 D845A over-expression treated with 1µM Ful + 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S176 [S176_L869R.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S236 [S236_S653C.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S8 [S8_GFP.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S249 [S249_L755S.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S128 [S128_D845A.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S248 [S248_L755S.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S152 [S152_L755S.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S235 [S235_S653C.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S115 [S115_wt.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S103 [S103_GFP.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S151 [S151_L755S.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S79 [S79_L869R.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S43 [S43_S653C.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S271 [S271_L869R.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S31 [S31_D845A.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S199 [S199_GFP.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S91 [S91_ESR1.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S223 [S223_D845A.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S163 [S163_V777L.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S7 [S7_GFP.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S175 [S175_L869R.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S32 [S32_D845A.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S284 [S284_ESR1.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S67 [S67_V777L.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S260 [S260_V777L.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S55 [S55_L755S.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S139 [S139_S653C.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S211 [S211_wt.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S19 [S19_wt.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S259 [S259_V777L.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S283 [S283_ESR1.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S247 [S247_L755S.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S127 [S127_D845A.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S42 [S42_S653C.Ner]

description: Parental cell line with HER2 S653C over-expression treated with 1µM Ner

value: 1microM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S6 [S6_GFP.Ner]

description: Parental cell line with GFP over-expression treated with 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S78 [S78_L869R.Ner]

description: Parental cell line with HER2 L869R over-expression treated with 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S30 [S30_D845A.Ner]

description: Parental cell line with HER2 D845A over-expression treated with 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S270 [S270_L869R.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S162 [S162_V777L.Ner]

description: Parental cell line with HER2 V777L over-expression treated with 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S102 [S102_GFP.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S150 [S150_L755S.Ner]

description: Parental cell line with HER2 L755S over-expression treated with 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S114 [S114_wt.Ner]

description: Parental cell line with HER2 wt over-expression treated with 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S222 [S222_D845A.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S282 [S282_ESR1.Ner]

description: Parental cell line with ESR1 Y537S over-expression treated with 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S18 [S18_wt.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S258 [S258_V777L.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S138 [S138_S653C.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S187 [S187_ESR1.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S126 [S126_D845A.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S246 [S246_L755S.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S198 [S198_GFP.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S210 [S210_wt.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S174 [S174_L869R.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S54 [S54_L755S.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S41 [S41_S653C.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S161 [S161_V777L.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S53 [S53_L755S.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S281 [S281_ESR1.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S65 [S65_V777L.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S77 [S77_L869R.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S113 [S113_wt.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S234 [S234_S653C.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S149 [S149_L755S.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S29 [S29_D845A.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S197 [S197_GFP.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S5 [S5_GFP.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S90 [S90_ESR1.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S66 [S66_V777L.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S257 [S257_V777L.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S173 [S173_L869R.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S17 [S17_wt.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S185 [S185_ESR1.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S125 [S125_D845A.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S89 [S89_ESR1.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S269 [S269_L869R.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S137 [S137_S653C.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S245 [S245_L755S.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S40 [S40_S653C.Fulv]

description: Parental cell line with HER2 S653C over-expression treated with 1µM Fulv

value: 1microM Fulv


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S233 [S233_S653C.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S101 [S101_GFP.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S232 [S232_S653C.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S209 [S209_wt.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S268 [S268_L869R.Fulv]

description: Parental cell line with HER2 L869R over-expression treated with 1µM Fulv


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S186 [S186_ESR1.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S4 [S4_GFP.Fulv]

description: Parental cell line with GFP over-expression treated with 1µM Fulv


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S160 [S160_V777L.Fulv]

description: Parental cell line with HER2 V777L over-expression treated with 1µM Fulv


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S16 [S16_wt.Fulv]

description: Parental cell line with HER2 wt over-expression treated with 1µM Fulv


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S76 [S76_L869R.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S52 [S52_L755S.Fulv]

description: Parental cell line with HER2 L755S over-expression treated with 1µM Fulv


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S220 [S220_D845A.Fulv]

description: Parental cell line with HER2 D845A over-expression treated with 1µM Fulv


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S280 [S280_ESR1.Fulv]

description: Parental cell line with ESR1 Y537S over-expression treated with 1µM Fulv


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S136 [S136_S653C.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S184 [S184_ESR1.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S221 [S221_D845A.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S28 [S28_D845A.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S88 [S88_ESR1.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S112 [S112_wt.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S64 [S64_V777L.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S172 [S172_L869R.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S244 [S244_L755S.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S124 [S124_D845A.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S196 [S196_GFP.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S256 [S256_V777L.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S208 [S208_wt.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S231 [S231_S653C.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S159 [S159_V777L.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S100 [S100_GFP.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S123 [S123_D845A.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S39 [S39_S653C.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S51 [S51_L755S.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S195 [S195_GFP.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S147 [S147_L755S.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S63 [S63_V777L.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S279 [S279_ESR1.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S15 [S15_wt.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S243 [S243_L755S.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S267 [S267_L869R.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S183 [S183_ESR1.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S75 [S75_L869R.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S135 [S135_S653C.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S3 [S3_GFP.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S255 [S255_V777L.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S207 [S207_wt.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S266 [S266_L869R.DMSO]

description: Parental cell line with HER2 L869R over-expression treated with DMSO

value: DMSO


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S111 [S111_wt.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S219 [S219_D845A.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S148 [S148_L755S.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S171 [S171_L869R.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S134 [S134_S653C.DMSO]

description: Parental cell line with HER2 S653C over-expression treated with DMSO


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S87 [S87_ESR1.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S278 [S278_ESR1.DMSO]

description: Parental cell line with ESR1 Y537S over-expression treated with DMSO


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S242 [S242_L755S.DMSO]

description: Parental cell line with HER2 L755S over-expression treated with DMSO


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S38 [S38_S653C.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S74 [S74_L869R.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S194 [S194_GFP.DMSO]

description: Parental cell line with GFP over-expression treated with DMSO


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S254 [S254_V777L.DMSO]

description: Parental cell line with HER2 V777L over-expression treated with DMSO


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S2 [S2_GFP.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S62 [S62_V777L.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S26 [S26_D845A.DMSO]

description: Parental cell line with HER2 D845A over-expression treated with DMSO


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S98 [S98_GFP.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S50 [S50_L755S.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S230 [S230_S653C.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S27 [S27_D845A.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S146 [S146_L755S.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S122 [S122_D845A.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S86 [S86_ESR1.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S14 [S14_wt.DMSO]

description: Parental cell line with HER2 wt over-expression treated with DMSO


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S110 [S110_wt.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S99 [S99_GFP.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S182 [S182_ESR1.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S218 [S218_D845A.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S206 [S206_wt.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S181 [S181_ESR1.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S145 [S145_L755S.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S217 [S217_D845A.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S25 [S25_D845A.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S73 [S73_L869R.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S170 [S170_L869R.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S277 [S277_ESR1.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S133 [S133_S653C.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S157 [S157_V777L.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S241 [S241_L755S.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S121 [S121_D845A.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S265 [S265_L869R.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S13 [S13_wt.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S253 [S253_V777L.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S37 [S37_S653C.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S49 [S49_L755S.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S158 [S158_V777L.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S169 [S169_L869R.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S229 [S229_S653C.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S97 [S97_GFP.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S193 [S193_GFP.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S61 [S61_V777L.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S85 [S85_ESR1.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S109 [S109_wt.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S1 [S1_GFP.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S288 [S288_ESR1.Fulv+Palbo]

description: Parental cell line with ESR1 Y537S over-expression treated with 1µM Fulv + 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S180 [S180_L869R.Fulv+Palbo]

description: Parental cell line with HER2 L869R over-expression treated with 1µM Fulv + 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S96 [S96_ESR1.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S84 [S84_L869R.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S204 [S204_GFP.Fulv+Palbo]

description: Parental cell line with GFP over-expression treated with 1µM Fulv + 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S60 [S60_L755S.Fulv+Palbo]

description: Parental cell line with HER2 L755S over-expression treated with 1µM Fulv + 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S240 [S240_S653C.Fulv+Palbo]

description: Parental cell line with HER2 S653C over-expression treated with 1µM Fulv + 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S228 [S228_D845A.Fulv+Palbo]

description: Parental cell line with HER2 D845A over-expression treated with 1µM Fulv + 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S120 [S120_wt.Fulv+Palbo]

description: Parental cell line with HER2 wt over-expression treated with 1µM Fulv + 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S252 [S252_L755S.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S36 [S36_D845A.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S24 [S24_wt.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S48 [S48_S653C.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S276 [S276_L869R.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S168 [S168_V777L.Fulv+Palbo]

description: Parental cell line with HER2 V777L over-expression treated with 1µM Fulv + 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S156 [S156_L755S.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S216 [S216_wt.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S108 [S108_GFP.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_3_S205 [S205_wt.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S144 [S144_S653C.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S71 [S71_V777L.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S119 [S119_wt.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S192 [S192_ESR1.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S107 [S107_GFP.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S203 [S203_GFP.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S275 [S275_L869R.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S167 [S167_V777L.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S179 [S179_L869R.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S59 [S59_L755S.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S239 [S239_S653C.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S191 [S191_ESR1.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S287 [S287_ESR1.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S263 [S263_V777L.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S35 [S35_D845A.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S72 [S72_V777L.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S132 [S132_D845A.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S227 [S227_D845A.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S12 [S12_GFP.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S131 [S131_D845A.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S264 [S264_V777L.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S95 [S95_ESR1.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S47 [S47_S653C.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S11 [S11_GFP.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S215 [S215_wt.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S251 [S251_L755S.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S155 [S155_L755S.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S23 [S23_wt.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S70 [S70_V777L.Palbo]

description: Parental cell line with HER2 V777L over-expression treated with 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S178 [S178_L869R.Palbo]

description: Parental cell line with HER2 L869R over-expression treated with 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S143 [S143_S653C.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S202 [S202_GFP.Palbo]

description: Parental cell line with GFP over-expression treated with 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S82 [S82_L869R.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S83 [S83_L869R.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S118 [S118_wt.Palbo]

description: Parental cell line with HER2 wt over-expression treated with 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S34 [S34_D845A.Palbo]

description: Parental cell line with HER2 D845A over-expression treated with 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S286 [S286_ESR1.Palbo]

description: Parental cell line with ESR1 Y537S over-expression treated with 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S106 [S106_GFP.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S214 [S214_wt.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S22 [S22_wt.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S154 [S154_L755S.Palbo]

description: Parental cell line with HER2 L755S over-expression treated with 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S10 [S10_GFP.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S142 [S142_S653C.Palbo]

description: Parental cell line with HER2 S653C over-expression treated with 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S274 [S274_L869R.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S226 [S226_D845A.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S94 [S94_ESR1.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S166 [S166_V777L.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S250 [S250_L755S.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S262 [S262_V777L.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S238 [S238_S653C.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S190 [S190_ESR1.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S81 [S81_L869R.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S58 [S58_L755S.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S141 [S141_S653C.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S189 [S189_ESR1.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S93 [S93_ESR1.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S33 [S33_D845A.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S130 [S130_D845A.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S165 [S165_V777L.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S177 [S177_L869R.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S201 [S201_GFP.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S261 [S261_V777L.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S225 [S225_D845A.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S117 [S117_wt.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S9 [S9_GFP.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S69 [S69_V777L.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S46 [S46_S653C.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S105 [S105_GFP.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S237 [S237_S653C.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S129 [S129_D845A.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S153 [S153_L755S.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S285 [S285_ESR1.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S273 [S273_L869R.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S21 [S21_wt.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S57 [S57_L755S.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S80 [S80_L869R.Fulv+Ner]

description: Parental cell line with HER2 L869R over-expression treated with 1µM Ful + 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S44 [S44_S653C.Fulv+Ner]

description: Parental cell line with HER2 S653C over-expression treated with 1µM Ful + 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S152 [S152_L755S.Fulv+Ner]

description: Parental cell line with HER2 L755S over-expression treated with 1µM Ful + 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S20 [S20_wt.Fulv+Ner]

description: Parental cell line with HER2 wt over-expression treated with 1µM Ful + 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S200 [S200_GFP.Fulv+Ner]

description: Parental cell line with GFP over-expression treated with 1µM Ful + 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S164 [S164_V777L.Fulv+Ner]

description: Parental cell line with HER2 V777L over-expression treated with 1µM Ful + 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S68 [S68_V777L.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S56 [S56_L755S.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S272 [S272_L869R.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S45 [S45_S653C.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S188 [S188_ESR1.Fulv+Ner]

description: Parental cell line with ESR1 Y537S over-expression treated with 1µM Ful + 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S32 [S32_D845A.Fulv+Ner]

description: Parental cell line with HER2 D845A over-expression treated with 1µM Ful + 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S116 [S116_wt.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S260 [S260_V777L.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S92 [S92_ESR1.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S284 [S284_ESR1.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S212 [S212_wt.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S140 [S140_S653C.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S213 [S213_wt.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S176 [S176_L869R.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S8 [S8_GFP.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S236 [S236_S653C.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S249 [S249_L755S.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S103 [S103_GFP.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S248 [S248_L755S.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S128 [S128_D845A.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S235 [S235_S653C.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S43 [S43_S653C.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S115 [S115_wt.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S31 [S31_D845A.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S91 [S91_ESR1.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S151 [S151_L755S.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S104 [S104_GFP.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S223 [S223_D845A.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S271 [S271_L869R.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S199 [S199_GFP.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S163 [S163_V777L.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S175 [S175_L869R.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S67 [S67_V777L.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S7 [S7_GFP.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S55 [S55_L755S.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S139 [S139_S653C.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S224 [S224_D845A.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S19 [S19_wt.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S187 [S187_ESR1.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S283 [S283_ESR1.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S127 [S127_D845A.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S259 [S259_V777L.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S162 [S162_V777L.Ner]

description: Parental cell line with HER2 V777L over-expression treated with 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S211 [S211_wt.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S79 [S79_L869R.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S270 [S270_L869R.Ner]

description: Parental cell line with HER2 L869R over-expression treated with 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S6 [S6_GFP.Ner]

description: Parental cell line with GFP over-expression treated with 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S30 [S30_D845A.Ner]

description: Parental cell line with HER2 D845A over-expression treated with 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S150 [S150_L755S.Ner]

description: Parental cell line with HER2 L755S over-expression treated with 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S234 [S234_S653C.Ner]

description: Parental cell line with HER2 S653C over-expression treated with 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S78 [S78_L869R.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S102 [S102_GFP.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S222 [S222_D845A.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S114 [S114_wt.Ner]

description: Parental cell line with HER2 wt over-expression treated with 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S66 [S66_V777L.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S18 [S18_wt.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S138 [S138_S653C.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S90 [S90_ESR1.Ner]

description: Parental cell line with ESR1 Y537S over-expression treated with 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S198 [S198_GFP.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S258 [S258_V777L.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S210 [S210_wt.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S246 [S246_L755S.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S174 [S174_L869R.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S247 [S247_L755S.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S42 [S42_S653C.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S54 [S54_L755S.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S41 [S41_S653C.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S161 [S161_V777L.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S53 [S53_L755S.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S77 [S77_L869R.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S65 [S65_V777L.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S197 [S197_GFP.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S149 [S149_L755S.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S113 [S113_wt.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S29 [S29_D845A.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S281 [S281_ESR1.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S257 [S257_V777L.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S5 [S5_GFP.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S282 [S282_ESR1.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S126 [S126_D845A.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S221 [S221_D845A.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S173 [S173_L869R.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S185 [S185_ESR1.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S101 [S101_GFP.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S233 [S233_S653C.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S125 [S125_D845A.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S89 [S89_ESR1.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S137 [S137_S653C.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S209 [S209_wt.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S245 [S245_L755S.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S269 [S269_L869R.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S186 [S186_ESR1.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S40 [S40_S653C.Fulv]

description: Parental cell line with HER2 S653C over-expression treated with 1µM Fulv


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S4 [S4_GFP.Fulv]

description: Parental cell line with GFP over-expression treated with 1µM Fulv


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S16 [S16_wt.Fulv]

description: Parental cell line with HER2 wt over-expression treated with 1µM Fulv


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S136 [S136_S653C.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S232 [S232_S653C.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S17 [S17_wt.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S184 [S184_ESR1.Fulv]

description: Parental cell line with ESR1 Y537S over-expression treated with 1µM Fulv


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S76 [S76_L869R.Fulv]

description: Parental cell line with HER2 L869R over-expression treated with 1µM Fulv


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S172 [S172_L869R.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S220 [S220_D845A.Fulv]

description: Parental cell line with HER2 D845A over-expression treated with 1µM Fulv


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S280 [S280_ESR1.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S28 [S28_D845A.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S112 [S112_wt.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S64 [S64_V777L.Fulv]

description: Parental cell line with HER2 V777L over-expression treated with 1µM Fulv


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S244 [S244_L755S.Fulv]

description: Parental cell line with HER2 L755S over-expression treated with 1µM Fulv


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S124 [S124_D845A.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S196 [S196_GFP.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S256 [S256_V777L.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S88 [S88_ESR1.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S100 [S100_GFP.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S148 [S148_L755S.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S208 [S208_wt.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S268 [S268_L869R.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S39 [S39_S653C.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S231 [S231_S653C.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S159 [S159_V777L.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S15 [S15_wt.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S51 [S51_L755S.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S195 [S195_GFP.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S63 [S63_V777L.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S160 [S160_V777L.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S52 [S52_L755S.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S279 [S279_ESR1.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S243 [S243_L755S.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S267 [S267_L869R.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S75 [S75_L869R.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S27 [S27_D845A.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S183 [S183_ESR1.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S135 [S135_S653C.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S3 [S3_GFP.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S255 [S255_V777L.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S171 [S171_L869R.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S207 [S207_wt.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S99 [S99_GFP.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S123 [S123_D845A.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S87 [S87_ESR1.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S266 [S266_L869R.DMSO]

description: Parental cell line with HER2 L869R over-expression treated with DMSO


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S111 [S111_wt.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S134 [S134_S653C.DMSO]

description: Parental cell line with HER2 S653C over-expression treated with DMSO


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S278 [S278_ESR1.DMSO]

description: Parental cell line with ESR1 Y537S over-expression treated with DMSO


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S219 [S219_D845A.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S74 [S74_L869R.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S242 [S242_L755S.DMSO]

description: Parental cell line with HER2 L755S over-expression treated with DMSO


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S147 [S147_L755S.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S194 [S194_GFP.DMSO]

description: Parental cell line with GFP over-expression treated with DMSO


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S38 [S38_S653C.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S254 [S254_V777L.DMSO]

description: Parental cell line with HER2 V777L over-expression treated with DMSO


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S26 [S26_D845A.DMSO]

description: Parental cell line with HER2 D845A over-expression treated with DMSO


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S2 [S2_GFP.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S158 [S158_V777L.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S62 [S62_V777L.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S98 [S98_GFP.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S122 [S122_D845A.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S86 [S86_ESR1.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S50 [S50_L755S.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S146 [S146_L755S.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S110 [S110_wt.DMSO]

description: Parental cell line with HER2 wt over-expression treated with DMSO


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S14 [S14_wt.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S230 [S230_S653C.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S182 [S182_ESR1.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S170 [S170_L869R.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S206 [S206_wt.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S145 [S145_L755S.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S181 [S181_ESR1.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S25 [S25_D845A.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S217 [S217_D845A.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S218 [S218_D845A.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S73 [S73_L869R.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S121 [S121_D845A.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S265 [S265_L869R.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S13 [S13_wt.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S277 [S277_ESR1.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S49 [S49_L755S.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S241 [S241_L755S.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S37 [S37_S653C.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S169 [S169_L869R.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S61 [S61_V777L.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S229 [S229_S653C.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S85 [S85_ESR1.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S193 [S193_GFP.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S1 [S1_GFP.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S97 [S97_GFP.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S288 [S288_ESR1.Fulv+Palbo]

description: Parental cell line with ESR1 Y537S over-expression treated with 1µM Fulv + 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S109 [S109_wt.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S180 [S180_L869R.Fulv+Palbo]

description: Parental cell line with HER2 L869R over-expression treated with 1µM Fulv + 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S205 [S205_wt.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S192 [S192_ESR1.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S96 [S96_ESR1.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S157 [S157_V777L.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S133 [S133_S653C.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S84 [S84_L869R.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S204 [S204_GFP.Fulv+Palbo]

description: Parental cell line with GFP over-expression treated with 1µM Fulv + 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S60 [S60_L755S.Fulv+Palbo]

description: Parental cell line with HER2 L755S over-expression treated with 1µM Fulv + 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S228 [S228_D845A.Fulv+Palbo]

description: Parental cell line with HER2 D845A over-expression treated with 1µM Fulv + 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S240 [S240_S653C.Fulv+Palbo]

description: Parental cell line with HER2 S653C over-expression treated with 1µM Fulv + 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S120 [S120_wt.Fulv+Palbo]

description: Parental cell line with HER2 wt over-expression treated with 1µM Fulv + 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan17_S253 [S253_V777L.DMSO]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S36 [S36_D845A.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S24 [S24_wt.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S276 [S276_L869R.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S48 [S48_S653C.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S132 [S132_D845A.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S168 [S168_V777L.Fulv+Palbo]

description: Parental cell line with HER2 V777L over-expression treated with 1µM Fulv + 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S156 [S156_L755S.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S12 [S12_GFP.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S72 [S72_V777L.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S108 [S108_GFP.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S264 [S264_V777L.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S144 [S144_S653C.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S216 [S216_wt.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S119 [S119_wt.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S179 [S179_L869R.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S107 [S107_GFP.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S71 [S71_V777L.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S83 [S83_L869R.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S203 [S203_GFP.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S143 [S143_S653C.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S275 [S275_L869R.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S59 [S59_L755S.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S239 [S239_S653C.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S167 [S167_V777L.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S287 [S287_ESR1.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S263 [S263_V777L.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S252 [S252_L755S.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S191 [S191_ESR1.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S35 [S35_D845A.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S131 [S131_D845A.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S227 [S227_D845A.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S215 [S215_wt.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S47 [S47_S653C.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S155 [S155_L755S.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S23 [S23_wt.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S70 [S70_V777L.Palbo]

description: Parental cell line with HER2 V777L over-expression treated with 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S251 [S251_L755S.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S190 [S190_ESR1.Palbo]

description: Parental cell line with ESR1 Y537S over-expression treated with 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S118 [S118_wt.Palbo]

description: Parental cell line with HER2 wt over-expression treated with 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S178 [S178_L869R.Palbo]

description: Parental cell line with HER2 L869R over-expression treated with 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S34 [S34_D845A.Palbo]

description: Parental cell line with HER2 D845A over-expression treated with 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S82 [S82_L869R.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S262 [S262_V777L.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S106 [S106_GFP.Palbo]

description: Parental cell line with GFP over-expression treated with 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S46 [S46_S653C.Palbo]

description: Parental cell line with HER2 S653C over-expression treated with 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S22 [S22_wt.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S10 [S10_GFP.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S142 [S142_S653C.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S274 [S274_L869R.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S95 [S95_ESR1.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S226 [S226_D845A.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S94 [S94_ESR1.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S250 [S250_L755S.Palbo]

description: Parental cell line with HER2 L755S over-expression treated with 10µM Palbo


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S11 [S11_GFP.Fulv+Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S166 [S166_V777L.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S58 [S58_L755S.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S130 [S130_D845A.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S189 [S189_ESR1.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S81 [S81_L869R.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S33 [S33_D845A.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S141 [S141_S653C.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S286 [S286_ESR1.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S93 [S93_ESR1.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S202 [S202_GFP.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S201 [S201_GFP.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S154 [S154_L755S.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S165 [S165_V777L.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S225 [S225_D845A.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S177 [S177_L869R.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S214 [S214_wt.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S261 [S261_V777L.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S45 [S45_S653C.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S117 [S117_wt.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S238 [S238_S653C.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S9 [S9_GFP.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S69 [S69_V777L.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S105 [S105_GFP.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S213 [S213_wt.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S285 [S285_ESR1.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S153 [S153_L755S.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S129 [S129_D845A.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S273 [S273_L869R.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S21 [S21_wt.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S57 [S57_L755S.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S249 [S249_L755S.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S80 [S80_L869R.Fulv+Ner]

description: Parental cell line with HER2 L869R over-expression treated with 1µM Ful + 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S200 [S200_GFP.Fulv+Ner]

description: Parental cell line with GFP over-expression treated with 1µM Ful + 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S20 [S20_wt.Fulv+Ner]

description: Parental cell line with HER2 wt over-expression treated with 1µM Ful + 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S164 [S164_V777L.Fulv+Ner]

description: Parental cell line with HER2 V777L over-expression treated with 1µM Ful + 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S56 [S56_L755S.Fulv+Ner]

description: Parental cell line with HER2 L755S over-expression treated with 1µM Ful + 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S188 [S188_ESR1.Fulv+Ner]

description: Parental cell line with ESR1 Y537S over-expression treated with 1µM Ful + 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S44 [S44_S653C.Fulv+Ner]

description: Parental cell line with HER2 S653C over-expression treated with 1µM Ful + 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S116 [S116_wt.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S272 [S272_L869R.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S32 [S32_D845A.Fulv+Ner]

description: Parental cell line with HER2 D845A over-expression treated with 1µM Ful + 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S68 [S68_V777L.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S284 [S284_ESR1.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S260 [S260_V777L.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S140 [S140_S653C.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S92 [S92_ESR1.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S104 [S104_GFP.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S224 [S224_D845A.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S176 [S176_L869R.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S212 [S212_wt.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S237 [S237_S653C.Palbo]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S236 [S236_S653C.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S128 [S128_D845A.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S103 [S103_GFP.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S248 [S248_L755S.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S235 [S235_S653C.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S115 [S115_wt.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S152 [S152_L755S.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S79 [S79_L869R.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S43 [S43_S653C.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S31 [S31_D845A.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S91 [S91_ESR1.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S151 [S151_L755S.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S271 [S271_L869R.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S223 [S223_D845A.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S199 [S199_GFP.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S55 [S55_L755S.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S139 [S139_S653C.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S163 [S163_V777L.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S67 [S67_V777L.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S19 [S19_wt.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S8 [S8_GFP.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S187 [S187_ESR1.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S127 [S127_D845A.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S259 [S259_V777L.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S211 [S211_wt.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S283 [S283_ESR1.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S247 [S247_L755S.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S6 [S6_GFP.Ner]

description: Parental cell line with GFP over-expression treated with 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S78 [S78_L869R.Ner]

description: Parental cell line with HER2 L869R over-expression treated with 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S162 [S162_V777L.Ner]

description: Parental cell line with HER2 V777L over-expression treated with 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S30 [S30_D845A.Ner]

description: Parental cell line with HER2 D845A over-expression treated with 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S175 [S175_L869R.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S7 [S7_GFP.Fulv+Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S102 [S102_GFP.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S270 [S270_L869R.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S150 [S150_L755S.Ner]

description: Parental cell line with HER2 L755S over-expression treated with 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S282 [S282_ESR1.Ner]

description: Parental cell line with ESR1 Y537S over-expression treated with 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S222 [S222_D845A.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S42 [S42_S653C.Ner]

description: Parental cell line with HER2 S653C over-expression treated with 1µM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S126 [S126_D845A.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S66 [S66_V777L.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S90 [S90_ESR1.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S18 [S18_wt.Ner]

description: Parental cell line with HER2 wt over-expression treated with 1µM Ner


title: F480low_12w_rep3

type: RNA

city: Stockholm

first: Ewoud

last: Ewing

address: CMM L8:05, Karolinska University Hospital

department: Clinical Neuroscience

country: Sweden

institute: Karolinska Institutet

hyb_protocol: GeneChip® ST Arrays (GeneChip® Mouse Gene 2.1 ST Array) were hybridized for 16 hours in a 45°C incubator, washed and stained

scan_protocol: Scanned on GeneTitan® Multi-Channel (MC) Instrument, according to the GeneTitan Instrument User Guide for Expression Arrays Plates (PN 702933 Rev 2. Affymetrix Inc., Santa Clara, CA).

data_processing: Data normalization, batch correction, and differential gene expression was performed using Partek Genomics Suite

label: biotin

organism: Mus musculus

source_name: Repopulated proliferating microglia (F4/80low) in Cx3cr1CreER/+R26DTA/+ mice, 12 weeks after Tamoxifen

label_protocol: RNA from each sample was used to generate amplified and biotinylated sense-strand cDNA from the entire expressed genome according to the GeneChip® WT Pico Reagent Kit User Manual (P/N 703262 Rev 1 Affymetrix Inc., Santa Clara, CA).

extract_protocol: Sorting strategies listed above. Cells were sorted into a solution of RNA later (Thermo Fisher Scientific). RNA was prepared using RNeasy Micro Kit (Qiagen). RNA quality and integrity was assessed using a Bioanalyzer 2100 (Agilent). All samples included had high quality (RIN = 7-10).

treatment_protocol: Naive cells were sorted from completely naive mice or TAM treated Cx3cr1CreER/+ control mice. Cx3cr1CreER/+R26DTA/+ mice were treated with Tamoxifen (TAM) on three consecutive days and cells sorted from the CNS on the indicated weeks. Cx3cr1CreER/+R26DTR/+ mice were treated with TAM separated by 48h, 3 weeks later treated with diphtheria toxin on three consecutive days and cells sorted 2 weeks later.

tag: strain

tag: treatment

value: Tamoxifen

tag: tissue

value: Brain

tag: sorting strategy

tag: batch


title: F480hi_4w_rep2

source_name: Repopulated CNS engrafting macrophage (F4/80hi) in Cx3cr1CreER/+R26DTA/+ mice, 4 weeks after Tamoxifen


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S138 [S138_S653C.Ner]


title: F480hi_12w_rep2

source_name: Repopulated CNS engrafting macrophage (F4/80hi) in Cx3cr1CreER/+R26DTA/+ mice, 12 weeks after Tamoxifen


title: DTR_2w_rep2

source_name: Repopulated proliferating microglia (F4/80low) in Cx3cr1CreER/+R26DTR/+ mice, 2 weeks after diphtheria toxin

value: Tamoxifen+diphtheria toxin


title: F480low_12w_rep2


title: F480low_4w_rep1

source_name: Repopulated proliferating microglia (F4/80low) in Cx3cr1CreER/+R26DTA/+ mice, 4 weeks after Tamoxifen


title: F480hi_12w_rep1


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S234 [S234_S653C.Ner]

value: HER2 S653C over-expression


title: F480low_4w_rep2


title: F480low_12w_rep1


title: F480low_4w_rep3


title: F480hi_4w_rep1


title: F480hi_12w_rep3


title: Naive_rep3

source_name: Naive microglia

value: Naive


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S198 [S198_GFP.Ner]


title: Naive_rep5


title: Naive_rep2


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S114 [S114_wt.Ner]


title: Naive_rep4


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S258 [S258_V777L.Ner]


title: Naive_rep1


title: Mono_rep5

source_name: Ly6Chi monocyte

value: Bone marrow

value: MACS BM mononcyte kit


title: DTR_2w_rep1


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S246 [S246_L755S.Ner]

value: HER2 L755S over-expression


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S174 [S174_L869R.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S54 [S54_L755S.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S186 [S186_ESR1.Ner]

value: ESR1 Y537S over-expression


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S161 [S161_V777L.Ner]

value: HER2 V777L over-expression


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S53 [S53_L755S.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S41 [S41_S653C.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S210 [S210_wt.Ner]


title: Post_D708rna_D502rna

first: Pierre

last: Larraufie

address: Box 289, Addenbrooke's Site

department: WT-MRC Institute of Metabolic Science

country: United Kingdom

institute: Metabolic Research Laboratories and MRC Metabolic Diseases Unit

data_processing:
data was demultiplexed using the Casava pipeline
data was mapped to the mouse genome GRCh38 using STAR v2.5.1 and raw counts generated simultaneously
Supplementary_files_format_and_content: Larraufie_VSG_EEC_human_rawcount.csv  is a csv file of number of counts for ensembl gene ids of each biological sample

source_name: jejunum enteorendocrine cells

growth_protocol: some jejunal part removed from patients undergoing gasterctomy Roux-en-Y reconstruction was collected or from jejunal biopsies 6 months after surgery

extract_protocol:
epithelium was single-cell digested, PFA fixed and stained with CHGA and SCG2 antibodies and FACS sorted for positive cells . RNA was extracted using a Ambion Recoverall Total nucleic acid isolation kit for FFPE
5ng of RNA was used for library construction using Clontech SMARTer Stranded Total RNA-Seq Kit – Pico Input Mammalian v1 (Takara Bio, USA) without fragmentation step
RNAseq SE-50 on an Illumina HiSEQ 40000

tag: stain

tag: time

value: post-surgery

value: jejunum

tag: cell type

value: enteroendocrine cells


title: Pre_D702rna_D502rna

value: pre-surgery


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S77 [S77_L869R.Ner]

value: HER2 L869R over-expression

value: 1microM Ner


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S65 [S65_V777L.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S113 [S113_wt.Ner]

value: HER2 wt over-expression


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S149 [S149_L755S.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S281 [S281_ESR1.Ner]


title: Post_D704rna_D502rna


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S29 [S29_D845A.Ner]

value: HER2 D845A over-expression


title: Post_D707rna_D502rna


title: Mono_rep3


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S197 [S197_GFP.Ner]

value: GFP over-expression


title: Mono_rep1


title: Mono_rep2


title: Intmac_rep1

source_name: Intestinal macrophage

value: Intestine


title: RPM_rep2

source_name: Red pulp macrophage

value: Spleen


title: CMP_rep1

source_name: Common myeloid progenitor

value: Naive


title: Mono_rep4

value: Bone marrow


title: GMP_rep1

source_name: Granulocyte macrophage progenitor


title: Pre_D703rna_D502rna


title: CMP_rep2


title: Post_D705rna_D502rna


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S5 [S5_GFP.Ner]


title: Pre_D701rna_D502rna


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S17 [S17_wt.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S257 [S257_V777L.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S185 [S185_ESR1.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S221 [S221_D845A.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S173 [S173_L869R.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S101 [S101_GFP.Ner]


title: Intmac_rep2


title: GMP_rep2


title: merged_D711_D503

data_processing:
data was demultiplexed using the Casava pipeline
data was mapped to the mouse genome GRCm38 using STAR v2.5.1 and raw counts generated simultaneously
Supplementary_files_format_and_content: Larraufie_VSG_EEC_mouse_rawcount.csv  is a csv file of number of counts for ensembl gene ids of each biological sample

growth_protocol: mice were grown on a 12h dark/light cycle with ad libitum access to water and standard chow

extract_protocol:
epithelium was single-cell digested and FACS sorted for positive cells. RNA was extracted using microplus RNeasy kit (Qiagen) and quality checked on a picochip bioanalyzer
2ng of RNA was used for library contruction using the SMARTer stranded total RNAseq kit v2 pico kit
RNAseq SE-50 on an Illumina HiSEQ 40000

treatment_protocol: VSG mice had ~70% of their stomach removed and sutured, sham were opened and had a suture around without pressure. A group of sham operated were weight matched for the VSG. All mice were fed on a standard chow except around the surgery for which they received liquid diet

tag: condition

value: VSG


title: merged_D709_D504


title: merged_D712_D504


title: merged_D704_D504


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S125 [S125_D845A.Ner]


title: merged_D711_D504


title: merged_D710_D504


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S269 [S269_L869R.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S89 [S89_ESR1.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S137 [S137_S653C.Ner]

value: HER2 S653C over-expression


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S245 [S245_L755S.Ner]

value: HER2 L755S over-expression


title: merged_D707_D504


title: RPM_rep1


title: merged_D710_D503


title: merged_D706_D504


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S40 [S40_S653C.Fulv]

description: Parental cell line with HER2 S653C over-expression treated with 1µM Fulv

value: 1microM Fulv


title: merged_D705_D504


title: merged_D708_D504


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S232 [S232_S653C.Fulv]


title: merged_D702_D504


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S16 [S16_wt.Fulv]

description: Parental cell line with HER2 wt over-expression treated with 1µM Fulv


title: merged_D712_D503


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S4 [S4_GFP.Fulv]

description: Parental cell line with GFP over-expression treated with 1µM Fulv


title: merged_D709_D503


title: merged_D702_D501


title: merged_D708_D503

value: ileum


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S233 [S233_S653C.Ner]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S136 [S136_S653C.Fulv]


title: merged_D703_D503


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S52 [S52_L755S.Fulv]

description: Parental cell line with HER2 L755S over-expression treated with 1µM Fulv


title: merged_D703_D504


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S160 [S160_V777L.Fulv]

description: Parental cell line with HER2 V777L over-expression treated with 1µM Fulv

value: HER2 V777L over-expression


title: merged_D704_D501

value: enteroendocrine cells


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S184 [S184_ESR1.Fulv]

description: Parental cell line with ESR1 Y537S over-expression treated with 1µM Fulv

value: ESR1 Y537S over-expression


title: merged_D703_D501


title: merged_D708_D501


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S76 [S76_L869R.Fulv]

description: Parental cell line with HER2 L869R over-expression treated with 1µM Fulv

value: HER2 L869R over-expression


title: merged_D707_D503


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S220 [S220_D845A.Fulv]

description: Parental cell line with HER2 D845A over-expression treated with 1µM Fulv


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S280 [S280_ESR1.Fulv]


title: merged_D706_D503


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S28 [S28_D845A.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S268 [S268_L869R.Fulv]


title: merged_D701_D503


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S172 [S172_L869R.Fulv]


title: merged_D707_D501


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S64 [S64_V777L.Fulv]


title: merged_D705_D501


title: merged_D705_D503


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S209 [S209_wt.Ner]

value: 1microM Ner


title: merged_D704_D503


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S124 [S124_D845A.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S112 [S112_wt.Fulv]

value: HER2 wt over-expression


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S88 [S88_ESR1.Fulv]


title: merged_D701_D504


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S244 [S244_L755S.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S196 [S196_GFP.Fulv]


title: merged_D702_D503


title: merged_D701_D501


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S148 [S148_L755S.Fulv]


title: merged_D712_D502

value: duodenum


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S256 [S256_V777L.Fulv]


title: merged_D711_D502


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S100 [S100_GFP.Fulv]

value: GFP over-expression


title: merged_D709_D502


title: M1-T284A-3

city: Houston

first: Samuel

last: Shelburne

state: TX

address: 1515 Holcombe Blvd

department: Infectious Diseases

institute: MD Anderson Cancer Center

data_processing:
The raw reads in FASTQ format were aligned to the reference genome, MGAS10870 (M3) or MGAS5005 (M1) using MOSAIK alignment software with parameters of -hs 15, -mmp 0.06, -mmal,   -minp 0.5, -act 35, -mhp 100, and -m all
The overlaps between aligned reads and annotated genes were counted using HTSeq software (http://www-huber.embl.de/users/anders/HTSeq/doc/overview.html). The gene counts were normalized using the scaling factor method implemented in the R package of DESeq2 (version 1.2.10). If the number of overlapped read of any given gene is less than one per million total mapped read for all samples, this gene was excluded from further analysis.
Please note that the MGAS10870 strain and its derivative strains get mapped to the publicly available MGAS315 genome  (NC_004070.1/AE014074.1).  For strain MGAS2221 and its derivatives, we map to the publicly available strain MGAS5005 (NZ_CP000017.1/CP000017.2).   MGAS10870 and MGAS2221 are very closely related to MGAS10870 and MGAS2221, respectively.
A negative binomial generalized linear model was fit to each gene expression with strain type as covariates. Then a likelihood-ratio test was applied to examine if there is any difference in the expression of a gene among six strains. The Benjamini-Hochberg method was used to control false discovery rate (FDR). The pairwise comparisons were performed to compare the gene expressions between WT vs any other strain using Wald's tests. The Holm's method was used to calculate adjusted p-value to correct for multiple testing. The calculation was conducted using R (version 3.0.1) with the package of DESeq2 (version 1.2.10)
Genome_build: MGAS315  (NC_004070.1/AE014074.1) and MGAS5005 (NZ_CP000017.1/CP000017.2) genomes
Supplementary_files_format_and_content: normalize counts

organism: Streptococcus sp. 'group A'

source_name: bacteria grown in THY

growth_protocol: GAS strains grown in THY late-exponential phase

extract_protocol:
Cells were lysed using a fast FastPrep approach and RNA was isolated from ruptured cell using an RNeasy Kit (Qiagen)
cDNA was fragmented to 200 bp (mean fragment size) with the S220 Focused-ultrasonicator (Covaris) and used to make barcoded sequencing libraries on the SPRI-TE Nucleic Acid Extractor (Beckman-Coulter).

treatment_protocol: RNA protect was added at 2x volumes to cells and then cells were harvested by centrifugation, snap frozen, and stored at -80 C.

tag: serotype

tag: molecule subtype

value: RNA after rRNA depletion


title: M1-E281A-4


title: M1-E281A-2


title: M1-D53A-6


title: M1-T284A-2


title: M1-T284A-4


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S208 [S208_wt.Fulv]


title: M1-D53A-3


title: merged_D707_D502


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S159 [S159_V777L.Fulv]


title: M1-T284A-1


title: merged_D706_D501


title: M1-D53A-1


title: M1-D53A-4


title: merged_D703_D502


title: M1-E281A-1


title: M1-wt-6


title: M1-E281A-3


title: merged_D702_D502


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S231 [S231_S653C.Fulv]


title: M1-wt-1


title: M1-wt-2


title: M3-T284A-3


title: merged_D710_D502


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S39 [S39_S653C.Fulv]


title: M3-T284A-4


title: M3-T284A-2


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S123 [S123_D845A.Fulv]

value: HER2 D845A over-expression


title: merged_D708_D502


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S63 [S63_V777L.Fulv]


title: M3-E281A-4


title: merged_D706_D502


title: M3-E281A-2


title: M1-wt-7


title: M3-T284A-1


title: merged_D701_D502


title: M3-E281A-3


title: M3-D53A-4


title: M3-D53A-6


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S15 [S15_wt.Fulv]


title: merged_D710_D501


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S51 [S51_L755S.Fulv]


title: M3-E281A-1


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S147 [S147_L755S.Fulv]


title: M3-D53A-1


title: M3-D53A-2


title: merged_D704_D502


title: M3-wt-5


title: merged_D705_D502


title: M3-wt-6


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S243 [S243_L755S.Fulv]


title: merged_D712_D501


title: M3-wt-1


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S279 [S279_ESR1.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S195 [S195_GFP.Fulv]


title: merged_D711_D501


title: 46CESC_BrdUIP_EarlyS

city: Tsu

first: Shin-ichiro

last: Takebayashi

state: Mie

address: 2-174, Edobashi

department: Graduate School of Medicine

country: Japan

institute: Mie University

library_source: genomic

data_processing:
Reads were mapped to the mm10 mouse reference genome using Bowtie2 and reads with MAPQ<20 were omitted from downstream analysis. Data analyses were performed using SeqMonk (http://www.bioinformatics.babraham.ac.uk/projects/seqmonk/) and R/Bioconductor (http://www.r-project.org; http://www.bioconductor.org). Numbers of total reads in each sample were normalized per million reads in SeqMonk. The sequence reads ratio between early and late S phase samples was determined in 5-Kb windows and loess-smoothed over a 300-Kb window size in R/Bioconductor. The smoothed dataset was used to generate a replication domain map.
Genome_build: mm10
Supplementary_files_format_and_content: txt

molecule: genomic DNA

source_name: BrdU-IP

growth_protocol: ES cells were maintained on gelatin-coated culture dishes in knockout Dulbecco’s modified eagle medium (Thermo Fisher Scientific, Cat#10829) supplemented with 7.5% ES-qualified FBS (GE Healthcare, Cat#SH30070.03E), 7.5% Knockout serum replacement (Thermo Fisher Scientific, Cat#10828-028), 2 mM L-glutamine (Thermo Fisher Scientific, Cat#25030–081), 10 mM Hepes (Thermo Fisher Scientific, Cat#15630–080), 100U/ml penicillin/streptomycin (Thermo Fisher Scientific, Cat#15070–063), 0.1 mM non-essential amino acids (Thermo Fisher Scientific, Cat#11140–050), 0.1 mM beta-mercaptoethanol (Thermo Fisher Scientific, Cat#21985–023), in the presence of leukemia inhibitory factor (LIF) at 37°C with 5% CO2.

extract_protocol:
Cells were washed twice with ice-cold PBS, trypsinized, and then fixed in 75% ethanol. These cells were resuspended in PBS containing 1% FBS, stained with propidium iodide (20 μg/ml) for 30 min in the presence of RNase A (250 μg/ml), and then were sorted into early and late S phase fractions by FACSCalibur flow cytometer (BD Biosciences). After phenol-chloroform extraction of DNA from 10,000 cells, sonication was performed to cut the DNA into an average size of about 100-600 bp. Then, immunoprecipitation with anti-BrdU mouse monoclonal antibody (BD Biosciences, #555627) was performed in each fraction to enrich BrdU-substituted replicating DNA. Isolated early and late replicating DNA was amplified individually by WGA (Sigma. SEQXE).
Libraries were constructed using Ion Plus Core Module for AB Library Builder System (Thermo Fisher Scientific, #4477683) with Ion Xpress Barcode Adapters 1-16 Kit (Thermo Fisher Scientific, #4477683) according to the standard instruction.

treatment_protocol: Cells were labeled with 50 µM BrdU for 2 h before harvest.

value: mouse embryonic stem cells

tag: replication domain

value: early replicating chromosomal regions

tag: ip antibody

value: BrdU (BD Biosciences, 555627)


title: 46CESC_BrdUIP_LateS

value: late replicating chromosomal regions


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S267 [S267_L869R.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S183 [S183_ESR1.Fulv]


title: Strain 660 day 1 S1

city: Cleveland

first: Hee-Sook

last: Kim

state: OH

address: 2399 Euclid Ave

institute: Cleveland State University

description:
processed data file:
BP_MFAseq_sliding window.txt
10_BPd1_S1

data_processing:
sequence reads were quality trimmed using with parameter,  trim_galore --phred33 -q 20 -a GATCGGAAGAGCACACGTCTGAACTCCAGTCAC --stringency 5 --length 20 --e 0.1
bowtie aligned to the reference genome (Tb927v5 from the TritrypDB) with -p 8 -a --best -M 1 -v 2
Aligned reads were quantified in Seqmonk and RPKM or RPM values were generated
For VSG and all genes, Raw reads were obtained from Seqmonk, then analyzed with DEseq.
Genome_build: Tb927v5 (http://tritrypdb.org/tritrypdb); VSGnome (http://tryps.rockefeller.edu); 14 BES fasta [PMID 18953401]
Supplementary_files_format_and_content: tab-delimited text files include RPKM or RPM values and DEseq output files

organism: Trypanosoma brucei

source_name: TbMCM-BP conditional knockout_day1

growth_protocol: Trypanosome cells were grown in HMI-9 media at 37C

extract_protocol:
for genomic DNA seq, cells were sorted by cell cycle using flow cytometry and genomic DNA was isolated.
for stranded RNAseq, NEBNext Ultra Directional RNA Library Prep Kit for Illumina
for MFAseq, genomic DNA was fragmented using the NEBNext dsDNA fragmentase (NEB) and sequencing libraries were generated using the NEBNext Ultra DNA Library prep kit and NEB multiplex oligos for Illumina
stranded RNAseq, MFA-seq (or Sort-seq)

treatment_protocol: Cells were treated 0, 1, and 2 days with tetracycline

tag: strain background

value: Lister 427 antigenic type MITat1.2 clone 221a

value: TbMCM-BP conditional knockout cell line # 660

tag: days with tetracycline


title: Strain 660 day 1 G1

description:
processed data file:
BP_MFAseq_sliding window.txt
9_BPd1_G1

tag: genotype/variation


title: Strain 660 day 1 S2

description:
processed data file:
BP_MFAseq_sliding window.txt
11_BPd1_S2


title: Strain 660 day 0 G2

description:
processed data file:
BP_MFAseq_sliding window.txt
8_BPd0_G2

source_name: TbMCM-BP conditional knockout_day0


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S75 [S75_L869R.Fulv]

source_name: Breast Cancer

tag: cell line

tag: agent

value: 1microM Fulv


title: Strain 660 day 1 G2

description:
processed data file:
BP_MFAseq_sliding window.txt
12_BPd1_G2


title: Strain 660 day 0 S2

description:
processed data file:
BP_MFAseq_sliding window.txt
7_BPd0_S2


title: Strain 660 day 0 S1

description:
processed data file:
BP_MFAseq_sliding window.txt
6_BPd0_S1


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S27 [S27_D845A.Fulv]


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S135 [S135_S653C.Fulv]

value: HER2 S653C over-expression


title: Strain 904 (WT) G2

description:
processed data file:
BP_MFAseq_sliding window.txt
4_wt_G2

source_name: WT cell line_day0

value: WT cell line #904


title: Strain 904 (WT) S2

description:
processed data file:
BP_MFAseq_sliding window.txt
3_wt_S2


title: T47D_Her2pos_populations.Rescue.seq.HER2.mut.Jan10_S3 [S3_GFP.Fulv]