title: Bacillus subtilis, DM, replicate 2

status: Public on Nov 09 2018

first: Tao

last: Pan

state: Illinois

address: 929 E. 57th Street

department: Biochemistry and Molecular Biology

description:
processed data file : BSub_Counts.xlsx
Bacillus subtilis, replicate 2, demethylase treated, which had tRNA subsequently isolated

data_processing:
We removed low-quality reads from the raw sequencing results using illumina-utils 17 (available from https://github.com/merenlab/illumina-utils). For the analysis of 16S rRNA gene amplicons data we used the program ‘iu-merge-pairs’ with default parameters, which merged partially overlapping paired-end Illumina reads while simultaneously removing any pair with more than 3 mismatches at the overlapped region. For pairs with three or less mismatches, we picked the base to be used in the final merged sequence from the read with the higher Q-score.
We used Minimum Entropy Decomposition 11 with default parameters to cluster high-quality 16S rRNA gene amplicons at 1-nt resolution, and GAST 18 to assign taxonomy to each read individually.
To recover tRNA sequences in this dataset we also used the program ‘iu-merge-pairs’, but with two additional flags and an additional parameter: --retain-only-overlap (to keep only the overlapping region while trimming tailing ends from both reads in a pair), --marker-gene-stringent (to align the reverse-complement of the second read even if the alignment occurs between the end of the second read and the beginning of the first read --which is a unique case for short inserts), and --max-num-mismatches 0 (to remove any pair if there was a disagreement between the aligned portions to increase the quality dramatically as described in 17).
We developed a new software tool to identify tRNA sequences in the raw sequencing results: tRNA-seq-tools (available from https://github.com/merenlab/tRNA-seq-tools). We used the tRNA-seq-tools program ‘trna-profile’ with default parameters to identify all sequences that matched our criteria for standard tRNA.
Supplementary_files_format_and_content: Bacterial tRNA counts in Excel file format

organism: Bacillus subtilis

source_name: bacterial culture

growth_protocol: We grew E. coli, B. subtilis, and S. aureus cultures at 37°C in Erlenmeyer flasks with a 10:1 flask medium ratio at 225rpm. We grew E. coli and B. subtilis in LB Lennox and S. aureus in tryptic soy broth. We grew B. viscericola in serum vials under strict anaerobic conditions at 37°C in chopped meat medium as previously described1. We harvested all cultures at midlog by centrifugation.

extract_protocol:
We lysed pelleted bacteria in 400μL of 0.3M NaOAc/HOAc, 10mM EDTA pH 4.8 with an equal volume acetate saturated phenol chloroform pH 4.8. We placed samples in a reciprocating bead beater for 45 seconds on  maximum intensity using FastPrep lysing matrix B. We centrifuged samples at 21,000rcf for 15  minutes at 4°C before re-extraction and ethanol precipitation of total RNA.Total tRNA was subsequently isolated using a denaturing 10% polyacrylamide gel followed by passive gel elution and ethanol precipitation.
Homemade protocol based on Illumina protocols. Deacylated RNAs with or without the demethylation were first treated with T4 Polynucleotide Kinase (Epicentre) at 37C° for 30 min to further warrant a free 3' hydroxyl group for template switching. Template-switching reactions were performed as described7. Briefly, we used an initial template-primer substrate consisting of a 41-nt RNA oligonucleotide (5’-AGA UCG GAA GAG CAC ACG UCU AGU UCU ACA GUC CGA CGA UC/3SpC3/-3’) that contains Illumina Read1 and Read2 primer-binding sites and a 3’ blocking group (three carbon spacer; IDT) annealed to a complementary 32P-labeled DNA primer with a single-nucleotide 3’ overhang, T, which facilitates the template switch to full-length tRNA that mostly contain a 3’ CCA end. For g2RT template-switching reactions, average 100 ng of demethylated tRNAs or 1 μg of demethylated total RNA were mixed with the initial template-primer substrate (100 nM) and g2RT (1 μM of TeI4cΔEn or 500 nM of GsI-IIC RT) in reaction medium containing 450 mM NaCl, 5 mM MgCl2, 20 mM Tris-HCl, pH 7.5, and DTT (1 mM for TeI4cΔEn or 5 mM for GsI-IIC RT). For template-switching with TeI4cΔEn RT, the reactions were pre-incubated at room temperature for 30 min, initiated by adding 25 mM dNTPs to a final concentration of 1 mM and incubating at 60°C for 30 min. For template-switching with GsI-IIC RT, the pre-incubation step is not required. The reactions were terminated by adding 5 M NaOH to a final concentration of 0.25 M, incubating at 95°C for 3 min, and neutralizing with 5M HCl. The cDNAs resulting from template switching were analyzed in a denaturing 6% polyacrylamide gel, electroeluted using a D-tube Dialyzer Maxi with MWCO of 6-8 kDa (EMD Millipore), and ethanol precipitated with 0.3 M sodium acetate in the presence of 25 µg of linear acrylamide (Life Technologies). The purified cDNAs were then circularized with CircLigase II (Epicentre) using manufacture’s protocol with extended incubation time of 5 hours at 60°C, phenol-CIA extracted, ethanol precipitated and amplified with Phusion-HF (Thermo Scientific) using Illumina multiplex (5’- AAT GAT ACG GCG ACC ACC GAG ATC TAC ACG TTC AGA GTT CTA CAG TCC GAC GAT C -3’) and barcode (5’- CAA GCA GAA GAC GGC ATA CGA GAT BARCODE GTG ACT GGA GTT CAG ACG TGT GCT CTT CCG ATC T -3’) primers for 12 cycles of 98°C for 5 sec, 60°C for 10 sec and 72°C for 10 sec. The PCR products were sequenced on Illumina HiSeq 2000 system.

treatment_protocol: Sequenced tRNA was derived from bacterial pellets. DM refers to demethylase treatment. This is a cocktail of AlkB and AlkB engineered mutants that remove modifications from the tRNAs. This treatment was performed after isolation of tRNA from mouse cecum.

value: demethylase

value: bacterial culture

tag: molecule subtype

value: tRNA


title: Bacillus subtilis, DM, replicate 1

type: SRA

city: Chicago

country: USA

institute: University of Chicago

description:
processed data file : BSub_Counts.xlsx
Bacillus subtilis, replicate 1, demethylase treated, which had tRNA subsequently isolated

library_source: transcriptomic

molecule: total RNA

tag: treatment

tag: tissue


title: K562-plate6-96

city: Stanford

first: Howard

last: Chang

state: CA

address: 269 Campus Drive

department: Dermatology

institute: Stanford University

description: K562 cells were fixed, nuclei isolated and then permeabilized. Nuclei were then stained for GATA2 and transposed, then sorted into single wells of a 96 well plate for MI-ATAC

library_source: genomic

data_processing:
Paired-end reads were trimmed for Illumina adapter sequences and transposase sequences using an in-house script and mapped to mm9 using Bowtie2 v2.1.0 with parameters –very sensitive. Reads were subsequently filtered for alignment quality of > Q30 and were required to be properly paired. Duplicate reads were removed with Picard (http://picard.sourceforge.net) v1.79. Reads mapping to the mitochondria or chromosome Y were removed and not considered. Peak calling was performed by MACS2 with parameters --call-summits –q 0.05 –nomodel –shift 0. Peaks were filtered using the consensus excludable ENCODE blacklist ( http://hgdownload.cse.ucsc.edu/goldenPath/hg19/encodeDCC/wgEncodeMapability/) and a custom blacklist designed to remove high-signal-causing repeats and mitochondrial homologues. Overlapping peaks from all samples were merged together to a consensus peak list. Then peak region was extended to 500 bp around the summit.
Genome_build: hg19
Supplementary_files_format_and_content: Peak calling was performed by MACS2 with parameters --call-summits –q 0.05 –nomodel –shift 0. Peaks were filtered using the consensus excludable ENCODE blacklist ( http://hgdownload.cse.ucsc.edu/goldenPath/hg19/encodeDCC/wgEncodeMapability/) and a custom blacklist designed to remove high-signal-causing repeats and mitochondrial homologues. Overlapping peaks from all samples were merged together to a consensus peak list. Then peak region was extended to 500 bp around the summit.

molecule: genomic DNA

organism: Homo sapiens

source_name: K562 cells

extract_protocol: single cell MI-ATAC of K562 cells. Single cells were first fixed, then  lysed, followed by  transposition. The transposition reaction is the same as for standard ATAC-seq except with 0.05% Igepal CA-630 in the lysis buffer. After 30 min transposition, at 37 degrees, the reaction was quenched using 40 mM EDTA, then cells were centrifuged and supernatant was discarded. 20 µl of reverse crosslinking solution was added to the cell pellet (with final concentration of 5 ng/ml proteinase K).  Reverse crosslinking was performed 65 degree overnight and inactivated by a 10 min incubation at 80 degrees the next day. The 25 µl PCR master mix (NEB, M0541S) and 5 µl of two unique primer combinations were directly added to the reverse crosslinking mixture and PCR was performed as previous described. The PCR product was purified with Qiagen MinElute purification kit and eluted in 20 µl Qiagen EB elution buffer

tag: cell line

value: K562 cells

tag: antibody


title: Pancreas_AP_rep1

status: Public on Nov 10 2018

type: RNA

city: Shanghai

first: Jing

last: Lin

state: State...

address: Shanghai

country: China

institute: Red House Hospital

description: Gene expression in AP rat-pancreas tissues

hyb_protocol: 1 μg of each labeled cRNA was fragmented by adding 5 μl 10 × Blocking Agent and 1 μl of 25 × Fragmentation Buffer, then heated the mixture at 60 °C for 30 min, finally 25 μl 2 × GE Hybridization buffer was added to dilute the labeled cRNA. 50 μl of hybridization solution was dispensed into the gasket slide and assembled to the LncRNA expression microarray slide.

scan_protocol: The hybridized arrays were washed, fixed and scanned with using the Agilent DNA Microarray Scanner (part number G2505C).

data_processing: Agilent Feature Extraction software (version 11.0.1.1) was used to analyze acquired array images. Quantile normalization and subsequent data processing were performed with using the GeneSpring GX v12.1 software package (Agilent Technologies). After quantile normalization of the raw data, LncRNAs and mRNAs that at least 3 out of 6 samples have flags in Present or Marginal (“All Targets Value”) were chosen for further data analysis. Differentially expressed LncRNAs and mRNAs with statistical significance between the two groups were identified through P-value/FDR filtering. Differentially expressed LncRNAs and mRNAs between the two samples were identified through Fold Change filtering.

organism: Rattus norvegicus

source_name: pancreas, AP, replicate 1

label_protocol: Each sample was amplified and transcribed into fluorescent cRNA along the entire length of the transcripts without 3’ bias utilizing a random priming method (Arraystar Flash RNA Labeling Kit, Arraystar). The labeled cRNAs were purified by RNeasy Mini Kit (Qiagen). The concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000.

growth_protocol: Rats were habituated to a constant room temperature (25 ℃) under a 12:12 h light:dark cycle with ad libitum access to food and water.

extract_protocol: mRNA was purified from total RNA after removal of rRNA (mRNA-ONLY™ Eukaryotic mRNA Isolation Kit, Epicentre). RNA quantity and quality were measured by NanoDrop ND-1000.

treatment_protocol: Retrograde pancreatic ductal administration of 1 mL/kg of 4% sodium taurocholate was used to induce the AP model. Animals were sacrificed after 9 h. Total RNAs were extracted from the pancreas of each rat using TRIzol reagent.

value: pancreas

tag: gender

value: male

tag: age

value: 6 weeks


title: Pancreas_AP_rep3

source_name: pancreas, AP, replicate 3


title: KFlow

city: Yonago City

first: Takafumi

last: Nakamura

address: 86 Nishi-cho

department: Department of Biomedical Science

country: Japan

institute: Tottori University

description: SAMPLE 3

hyb_protocol: 0.6 μg of Cy3-labelled cRNA was fragmented at 60°C for 30 minutes in a reaction volume of 25 μl containing 1x Agilent fragmentation buffer and 2x Agilent blocking agent following the manufacturer’s instructions. On completion of the fragmentation reaction, 25 μl of 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to SurePrint G3 Human GE 8x60K Microarray Ver2.0 (Agilent Technologies) for 17 hours at 65°C in a rotating Agilent hybridization oven. After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent Technologies) and 1 minute with 37°C GE Wash buffer 2 (Agilent Technologies).

scan_protocol: Slides were scanned immediately after washing on the Agilent SureScan Microarray Scanner (G2600D) using one color scan setting for 8x60k array slides (Scan Area 61x21.6 mm, Scan resolution 3um, Dye channel is set to Green PMT is set to 100%).

data_processing:
The scanned images were analyzed with Feature Extraction Software (Agilent Technologies) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities.
Processed signal intensities were normalized by the global scaling method.Trimmed mean probe intensity was determined by removing 2% of the lower and the higher end of the probe intensities in order to calculate the scaling factor. Normalized signal intensities were then calculated from the target intensity on each array using the scaling factor, so that the trimmed mean target intensity of each array was arbitrarily set to 2500.

source_name: PTX-sensitive ovarian cancer cells

label_protocol: Cyanine3 (Cy3) labeled cRNA was prepared from 0.1 μg Total RNA using the Low Input Quick Amp Labeling Kit (Agilent Technologies) according to the manufacturer's instructions, followed by RNeasy column purification (QIAGEN). Dye incorporation and cRNA yield were checked with the NanoDrop ND-2000 Spectrophotometer.

extract_protocol: Total RNA from each cell line was extracted using the NucleoSpinRNA kit according to the manufacturer’s protocol (TAKARA BIO INC).

value: KF

tag: cell type

value: ovarian adenocarcinoma cell line

tag: paclitaxel (ptx) phenotype

value: PTX-sensitive


title: C57BL/6 Gut Microbiome with High Fat Diet, DM, Mouse3

description: High fat mouse, biological replicate 3, which had tRNA subsequently isolated

data_processing:
We removed low-quality reads from the raw sequencing results using illumina-utils 17 (available from https://github.com/merenlab/illumina-utils). For the analysis of 16S rRNA gene amplicons data we used the program ‘iu-merge-pairs’ with default parameters, which merged partially overlapping paired-end Illumina reads while simultaneously removing any pair with more than 3 mismatches at the overlapped region. For pairs with three or less mismatches, we picked the base to be used in the final merged sequence from the read with the higher Q-score
We used Minimum Entropy Decomposition 11 with default parameters to cluster high-quality 16S rRNA gene amplicons at 1-nt resolution, and GAST 18 to assign taxonomy to each read individually.
To recover tRNA sequences in this dataset we also used the program ‘iu-merge-pairs’, but with two additional flags and an additional parameter: --retain-only-overlap (to keep only the overlapping region while trimming tailing ends from both reads in a pair), --marker-gene-stringent (to align the reverse-complement of the second read even if the alignment occurs between the end of the second read and the beginning of the first read --which is a unique case for short inserts), and --max-num-mismatches 0 (to remove any pair if there was a disagreement between the aligned portions to increase the quality dramatically as described in 17)
We developed a new software tool to identify tRNA sequences in the raw sequencing results: tRNA-seq-tools (available from https://github.com/merenlab/tRNA-seq-tools). We used the tRNA-seq-tools program ‘trna-profile’ with default parameters to identify all sequences that matched our criteria for standard tRNA
Supplementary_files_format_and_content: Mapped tRNA sequences in text files as output from tRNA seq tools.

organism: mouse gut metagenome

source_name: C57BL/6 (Gut Microbiome)

growth_protocol: We bred C57BL/6 mice and maintained them under standard 12:12 h light/dark conditions at the University of Chicago. We fed age and litter-matched specific pathogen free (SPF) or germ free (GF) male C57Bl/6 mice between 8-12 weeks old either a purified low fat (LF; Harlan Teklad TD.00102) diet, high saturated milk fat (MF; Harlan Teklad TD.97222) diet, or high polyunsaturated fat (PUFA; Harlan Teklad TD.97223) diet for four weeks ad libitum. Diets were formulated by Harlan Teklad and compositions were published previously15. We γ-irradiated gnotobiotic diets and tested them for sterility prior to use. We anesthetized mice using 10 mg/ml ketamine/xylazine followed by exsanguination and cervical dislocation. We snap-froze cecal contents in liquid nitrogen and stored at -80°C.

extract_protocol:
Cecal contents were snap-frozen in liquid nitrogen and stored at -80°C. Cecal contents were lysed in 400μL of 0.3M NaOAc/HOAc, 10mM EDTA pH 4.8 with an equal volume acetate saturated phenol chloroform pH 4.8 and were placed in a reciprocating bead beater on max using for 45 seconds using FastPrep lysing matrix B. Purified total RNA was deacylated by incubating in 0.1 M tris-HCl, pH 9 at 37°C for 45 min. Total tRNA was subsequently isolated using a denaturing 10% polyacrylamide gel followed by passive gel elution and ethanol precipitation.
Homemade protocol based on Illumina protocols. Deacylated RNAs with or without the demethylation were first treated with T4 Polynucleotide Kinase (Epicentre) at 37C° for 30 min to further warrant a free 3' hydroxyl group for template switching. Template-switching reactions were performed as described7. Briefly, we used an initial template-primer substrate consisting of a 41-nt RNA oligonucleotide (5’-AGA UCG GAA GAG CAC ACG UCU AGU UCU ACA GUC CGA CGA UC/3SpC3/-3’) that contains Illumina Read1 and Read2 primer-binding sites and a 3’ blocking group (three carbon spacer; IDT) annealed to a complementary 32P-labeled DNA primer with a single-nucleotide 3’ overhang, T, which facilitates the template switch to full-length tRNA that mostly contain a 3’ CCA end. For g2RT template-switching reactions, average 100 ng of demethylated tRNAs or 1 μg of demethylated total RNA were mixed with the initial template-primer substrate (100 nM) and g2RT (1 μM of TeI4cΔEn or 500 nM of GsI-IIC RT) in reaction medium containing 450 mM NaCl, 5 mM MgCl2, 20 mM Tris-HCl, pH 7.5, and DTT (1 mM for TeI4cΔEn or 5 mM for GsI-IIC RT). For template-switching with TeI4cΔEn RT, the reactions were pre-incubated at room temperature for 30 min, initiated by adding 25 mM dNTPs to a final concentration of 1 mM and incubating at 60°C for 30 min. For template-switching with GsI-IIC RT, the pre-incubation step is not required. The reactions were terminated by adding 5 M NaOH to a final concentration of 0.25 M, incubating at 95°C for 3 min, and neutralizing with 5M HCl. The cDNAs resulting from template switching were analyzed in a denaturing 6% polyacrylamide gel, electroeluted using a D-tube Dialyzer Maxi with MWCO of 6-8 kDa (EMD Millipore), and ethanol precipitated with 0.3 M sodium acetate in the presence of 25 µg of linear acrylamide (Life Technologies). The purified cDNAs were then circularized with CircLigase II (Epicentre) using manufacture’s protocol with extended incubation time of 5 hours at 60°C, phenol-CIA extracted, ethanol precipitated and amplified with Phusion-HF (Thermo Scientific) using Illumina multiplex (5’- AAT GAT ACG GCG ACC ACC GAG ATC TAC ACG TTC AGA GTT CTA CAG TCC GAC GAT C -3’) and barcode (5’- CAA GCA GAA GAC GGC ATA CGA GAT BARCODE GTG ACT GGA GTT CAG ACG TGT GCT CTT CCG ATC T -3’) primers for 12 cycles of 98°C for 5 sec, 60°C for 10 sec and 72°C for 10 sec. The PCR products were sequenced on Illumina HiSeq 2000 or Illumina MiSeq system.

treatment_protocol:
Sequenced tRNA was derived from gut micrbiome from  age and litter-matched specific pathogen free (SPF) or germ free (GF) male C57Bl/6 mice between 8-12 weeks old fed either a purified low fat (LF; Harlan Teklad TD.00102) diet, high saturated milk fat (MF; Harlan Teklad TD.97222) diet, or high polyunsaturated fat (PUFA; Harlan Teklad TD.97223) diet for four weeks ad libitum.
DM refers to demethylase treatment. This is a cocktail of AlkB and AlkB engineered mutants that remove modifications from the tRNAs. This treatment was performed after isolation of tRNA from mouse cecum.

tag: diet

value: high fat

value: mouse cecum

tag: host

value: Mus musculus (mouse)

tag: host strain


title: C57BL/6 Gut Microbiome with Low Fat Diet, DM, Mouse1

description: Low fat mouse, biological replicate 1, which had tRNA subsequently isolated

value: low fat


title: C57BL/6 Gut Microbiome with High Fat Diet, DM, Mouse1

description: High fat mouse, biological replicate 1, which had tRNA subsequently isolated


title: C57BL/6 Gut Microbiome with Low Fat Diet, DM, Mouse3

description: Low fat mouse, biological replicate 3, which had tRNA subsequently isolated


title: K562-plate6-92


title: K562-plate6-94


title: C57BL/6 Gut Microbiome with Low Fat Diet, DM, Mouse2

description: Low fat mouse, biological replicate 2, which had tRNA subsequently isolated


title: Pancreas_AP_rep2

source_name: pancreas, AP, replicate 2


title: K562-plate6-91


title: C57BL/6 Gut Microbiome with High Fat Diet, DM, Mouse2

description: High fat mouse, biological replicate 2, which had tRNA subsequently isolated


title: Pancreas_C_rep3

description: Gene expression in control rat-pancreas tissues

source_name: pancreas, control, replicate 3


title: Mpjaz-1_vs_Tak-1_repl4

city: Madrid

first: Jose M.

last: Franco-Zorrilla

state: Madrid

address: Darwin, 3

department: Plant Molecular Genetics

country: Spain

institute: Centro Nacinal de Biotecnologia-CSIC

hyb_protocol: 825 ng of Cy3 and Cy5 probes were mixed and hybridized for 17 hours at 65ºC in GEx Hybridization Buffer HI-RPM in a hybridization oven. Arrays were washed according to the manufacturer’s instructions and dried by centrifugation

scan_protocol: Images were scanned at 2um resolution in an Agilent DNA Microarrays Scanner (G2565BA, Agilent Technologies) and  were with Feature Extraction 9.0 software (Agilent Technologies).

data_processing: Raw intensities were background-subtracted by NORMEXP method with a offset of 50. Signals (in log2 scale) were  normalized by LOWESS algorithm followed by quantile normalization

organism: Marchantia polymorpha

label_protocol: 200 ng of total RNA were reverse transcribed used T7 primer and the cDNA converted to aRNA using T7 RNA polymerase, incorporating cyanine 3- or cyanine 5-labeled CTP, according to 'Two color Microarray-Based Gene Expression Analysis Protocol' (Agilent)

growth_protocol: Marchantia polymorpha accession Takaragaike-1 (Tak-1; male) and mutant Mpjaz-1 were used in this work. Plants were subcultivated on 0.5 Gamborg’s B5 medium containing 1% agar for 3 weeks. Apical fragments of the plants, excluding gemma cups, were ground in liquid nitrogen prior RNA extraction. Every independent biological replicate contained 6-8 plants.

extract_protocol: Total RNA from Marchantia was extracted with either RNeasy Plant Mini Kit (Qiagen) or Favorgen Plant RNA Extraction and DNase on-column digestion (Qiagen) was performed to remove genomic DNA contamination.

tag: genotype/variation


title: K562-plate6-93


title: KFTXlow

description: SAMPLE 2

source_name: KFTXlow PTX-resistant ovarian cancer cells

value: PTX-resistant


title: Mpjaz-1_vs_Tak-1_repl3


title: KO_iWAT, rep1

city: Tsukuba

first: Yang

last: LIU

state: Ibaraki.

address: 3-1-1 Koyadai, Tsukuba, Ibaraki

institute: Riken

hyb_protocol: Fragmented and labelled cDNA was hybridized to GeneChip Mouse Gene 1.0 ST arrays (Affymetrix)

scan_protocol: Arrays were scanned using the Affymetrix GeneChip Scanner 3000 TG

data_processing: The raw (CEL) data were normalized using the RMA method in the R/ Bioconductor package affy, and the comparisons of gene expression were implemented using the linear models for microarray data (Limma) package

label: biotin

organism: Mus musculus

source_name: Inguinal white adipose tissue from male mice

label_protocol: Biotinylated cRNA were prepared by using the WT terminal labeling kit (Ambion) according the manufacturer’s manual.

growth_protocol: The ATF KO and wild-type (WT) littermates derived from Atf7 heterozygotes on a C57BL/6 background and maintained in the specific-pathogen-free(SPF), temperature-control mouse facility.

extract_protocol: Total RNA was extracted using TRIzol (Invitrogen) based on the manufacturer’s protocol.

treatment_protocol: Fresh inguinal white adipose tissues were dissected from 5-week-old male mice

tag: strain

tag: genotype

value: ATF7 KO

value: 5-week old


title: Pancreas_C_rep1

source_name: pancreas, control, replicate 1


title: Mpjaz-1_vs_Tak-1_repl2


title: Pancreas_C_rep2

source_name: pancreas, control, replicate 2


title: Mpjaz-1_vs_Tak-1_repl1


title: K562-plate6-90


title: K562-plate6-9


title: K562-plate6-89


title: K562-plate6-95


title: K562-plate6-88


title: K562-plate6-87


title: K562-plate6-86


title: K562-plate6-85


title: K562-plate6-82


title: K562-plate6-79


title: K562-plate6-84


title: K562-plate6-74


title: K562-plate6-80


title: K562-plate6-83


title: K562-plate6-69


title: K562-plate6-71


title: K562-plate6-78


title: K562-plate6-81


title: K562-plate6-67


title: K562-plate6-8


title: K562-plate6-68


title: K562-plate6-7


title: K562-plate6-66


title: K562-plate6-70


title: K562-plate6-75


title: K562-plate6-72


title: K562-plate6-77


title: K562-plate6-73


title: K562-plate6-62


title: K562-plate6-65


title: K562-plate6-63


title: K562-plate6-64


title: K562-plate6-61


title: K562-plate6-76


title: K562-plate6-60


title: K562-plate6-6


title: K562-plate6-59


title: K562-plate6-56


title: K562-plate6-58


title: K562-plate6-55


title: K562-plate6-53


title: K562-plate6-44


title: K562-plate6-57


title: K562-plate6-43


title: K562-plate6-49


title: K562-plate6-52


title: K562-plate6-5


title: K562-plate6-50


title: K562-plate6-45


title: K562-plate6-47


title: K562-plate6-40


title: K562-plate6-42


title: K562-plate6-46


title: K562-plate6-41


title: K562-plate6-51


title: K562-plate6-48


title: K562-plate6-39


title: K562-plate6-38


title: K562-plate6-4


title: K562-plate6-36


title: K562-plate6-37


title: K562-plate6-35


title: K562-plate6-32


title: K562-plate6-33


title: K562-plate6-54


title: K562-plate6-3


title: K562-plate6-29


title: K562-plate6-20


title: K562-plate6-2


title: K562-plate6-24


title: K562-plate6-31


title: K562-plate6-30


title: K562-plate6-19


title: K562-plate6-22


title: K562-plate6-26


title: K562-plate6-15


title: K562-plate6-28


title: K562-plate6-25


title: K562-plate6-27


title: K562-plate6-18


title: K562-plate6-14


title: K562-plate6-21


title: K562-plate6-23


title: K562-plate6-17


title: K562-plate6-12


title: K562-plate6-16


title: K562-plate6-13


title: K562-plate6-11


title: K562-plate6-10


title: K562-plate6-34


title: K562-plate6-1


title: K562-plate5-95


title: K562-plate5-92


title: K562-plate5-93


title: K562-plate5-94


title: K562-plate5-9


title: K562-plate5-89


title: K562-plate5-88


title: K562-plate5-90


title: K562-plate5-84


title: K562-plate5-85


title: K562-plate5-86


title: K562-plate5-91


title: K562-plate5-80


title: K562-plate5-81


title: K562-plate5-87


title: K562-plate5-83


title: K562-plate5-76


title: K562-plate5-8


title: K562-plate5-77


title: K562-plate5-96


title: K562-plate5-75


title: K562-plate5-74


title: K562-plate5-73


title: K562-plate5-7


title: K562-plate5-72


title: K562-plate5-71


title: K562-plate5-70


title: K562-plate5-69


title: K562-plate5-67


title: K562-plate5-82


title: K562-plate5-79


title: K562-plate5-66


title: K562-plate5-78


title: K562-plate5-6


title: K562-plate5-64


title: K562-plate5-62


title: K562-plate5-63


title: K562-plate5-61


title: K562-plate5-56


title: K562-plate5-54


title: K562-plate5-53


title: K562-plate5-55


title: K562-plate5-59


title: K562-plate5-58


title: K562-plate5-57


title: K562-plate5-51


title: K562-plate5-5


title: K562-plate5-50


title: K562-plate5-47


title: K562-plate5-68


title: K562-plate5-60


title: K562-plate5-52


title: K562-plate5-48


title: K562-plate5-46


title: K562-plate5-45


title: Bacillus subtilis, replicate 1

description:
processed data file : BSub_Counts.xlsx
Bacillus subtilis, replicate 1, which had tRNA subsequently isolated

value: no demethylase


title: K562-plate5-44


title: K562-plate5-65


title: K562-plate5-42


title: K562-plate5-41


title: K562-plate5-43


title: K562-plate5-40


title: K562-plate5-4


title: K562-plate5-39


title: K562-plate5-37


title: K562-plate5-38


title: K562-plate5-49


title: K562-plate5-24


title: K562-plate5-23


title: K562-plate5-32


title: K562-plate5-35


title: K562-plate5-21


title: K562-plate5-29


title: K562-plate5-34


title: K562-plate5-28


title: K562-plate5-3


title: K562-plate5-20


title: K562-plate5-33


title: K562-plate5-27


title: K562-plate5-30


title: K562-plate5-31


title: K562-plate5-25


title: K562-plate5-19


title: K562-plate5-26


title: K562-plate5-18


title: K562-plate5-17


title: K562-plate5-15


title: K562-plate5-36


title: K562-plate5-16


title: K562-plate5-14


title: K562-plate5-12


title: K562-plate5-13


title: K562-plate5-11


title: K562-plate5-1


title: K562-plate5-10


title: K562-plate5-22


title: K562-plate5-2


title: K562-plate4-96


title: K562-plate4-94


title: K562-plate4-89


title: K562-plate4-93


title: K562-plate4-85


title: K562-plate4-83


title: K562-plate4-82


title: K562-plate4-92


title: K562-plate4-90


title: K562-plate4-9


title: K562-plate4-95


title: K562-plate4-79


title: K562-plate4-87


title: K562-plate4-81


title: K562-plate4-91


title: K562-plate4-80


title: K562-plate4-78


title: K562-plate4-75


title: K562-plate4-76


title: K562-plate4-84


title: K562-plate4-88


title: K562-plate4-73


title: K562-plate4-8


title: K562-plate4-77


title: K562-plate4-72


title: K562-plate4-7


title: K562-plate4-68


title: K562-plate4-67


title: K562-plate4-70


title: K562-plate4-74


title: K562-plate4-62


title: K562-plate4-64


title: K562-plate4-65


title: K562-plate4-63


title: K562-plate4-59


title: K562-plate4-54


title: K562-plate4-56


title: K562-plate4-53


title: K562-plate4-60


title: K562-plate4-61


title: K562-plate4-86


title: K562-plate4-52


title: K562-plate4-71


title: K562-plate4-6


title: K562-plate4-58


title: K562-plate4-57


title: K562-plate4-55


title: K562-plate4-51


title: K562-plate4-49


title: K562-plate4-5


title: K562-plate4-48


title: K562-plate4-69


title: K562-plate4-66


title: K562-plate4-46


title: K562-plate4-47


title: K562-plate4-45


title: K562-plate4-50


title: K562-plate4-39


title: K562-plate4-43


title: K562-plate4-42


title: K562-plate4-36


title: K562-plate4-32


title: K562-plate4-30


title: K562-plate4-44


title: K562-plate4-26


title: K562-plate4-27


title: K562-plate4-28


title: K562-plate4-25


title: K562-plate4-41


title: K562-plate4-40


title: K562-plate4-2


title: K562-plate4-21


title: K562-plate4-38


title: K562-plate4-37


title: K562-plate4-31


title: K562-plate4-4


title: K562-plate4-29


title: K562-plate4-3


title: K562-plate4-33


title: K562-plate4-17


title: K562-plate4-22


title: K562-plate4-18


title: K562-plate4-20


title: K562-plate4-19


title: K562-plate4-34


title: K562-plate4-24


title: K562-plate4-12


title: K562-plate4-13


title: K562-plate4-10


title: K562-plate4-35


title: K562-plate4-16


title: GM_plate4-96 [GM_single_cell_unstimulated]

description: GM12878 cells were fixed, permeabilized and stained against CD19 and pNFkB, then sorted into single wells of a 96 well plate for MI-ATAC

source_name: GM12878 cells

extract_protocol: single cell MI-ATAC of GM12878 cells. Single cells were first fixed, then  lysed, followed by staining for respective antibodies and transposition. The transposition reaction is the same as for standard ATAC-seq except with 0.05% Igepal CA-630 in the lysis buffer. After 30 min transposition, at 37 degrees, the reaction was quenched using 40 mM EDTA, then cells were centrifuged and supernatant was discarded. 20 µl of reverse crosslinking solution was added to the cell pellet (with final concentration of 5 ng/ml proteinase K).  Reverse crosslinking was performed 65 degree overnight and inactivated by a 10 min incubation at 80 degrees the next day. The 25 µl PCR master mix (NEB, M0541S) and 5 µl of two unique primer combinations were directly added to the reverse crosslinking mixture and PCR was performed as previous described. The PCR product was purified with Qiagen MinElute purification kit and eluted in 20 µl Qiagen EB elution buffer

value: CD19 and pNFkB


title: K562-plate4-23


title: K562-plate4-1


title: K562-plate4-15


title: GM_plate4-9 [GM_single_cell_unstimulated]


title: GM_plate4-95 [GM_single_cell_unstimulated]


title: GM_plate4-94 [GM_single_cell_unstimulated]


title: GM_plate4-92 [GM_single_cell_unstimulated]


title: GM_plate4-93 [GM_single_cell_unstimulated]


title: GM_plate4-90 [GM_single_cell_unstimulated]


title: GM_plate4-91 [GM_single_cell_unstimulated]


title: GM_plate4-89 [GM_single_cell_unstimulated]


title: GM_plate4-85 [GM_single_cell_unstimulated]


title: GM_plate4-86 [GM_single_cell_unstimulated]


title: GM_plate4-83 [GM_single_cell_unstimulated]


title: GM_plate4-84 [GM_single_cell_unstimulated]


title: GM_plate4-87 [GM_single_cell_unstimulated]


title: GM_plate4-80 [GM_single_cell_unstimulated]


title: K562-plate4-14


title: GM_plate4-79 [GM_single_cell_unstimulated]


title: GM_plate4-7 [GM_single_cell_unstimulated]


title: GM_plate4-8 [GM_single_cell_unstimulated]


title: GM_plate4-76 [GM_single_cell_unstimulated]


title: GM_plate4-71 [GM_single_cell_unstimulated]


title: GM_plate4-82 [GM_single_cell_unstimulated]


title: GM_plate4-74 [GM_single_cell_unstimulated]


title: GM_plate4-75 [GM_single_cell_unstimulated]


title: GM_plate4-72 [GM_single_cell_unstimulated]


title: GM_plate4-73 [GM_single_cell_unstimulated]


title: K562-plate4-11


title: GM_plate4-78 [GM_single_cell_unstimulated]


title: GM_plate4-6 [GM_single_cell_unstimulated]


title: GM_plate4-70 [GM_single_cell_unstimulated]


title: GM_plate4-88 [GM_single_cell_unstimulated]


title: GM_plate4-69 [GM_single_cell_unstimulated]


title: GM_plate4-68 [GM_single_cell_unstimulated]


title: GM_plate4-65 [GM_single_cell_unstimulated]


title: GM_plate4-67 [GM_single_cell_unstimulated]


title: GM_plate4-66 [GM_single_cell_unstimulated]


title: GM_plate4-81 [GM_single_cell_unstimulated]


title: GM_plate4-62 [GM_single_cell_unstimulated]


title: GM_plate4-63 [GM_single_cell_unstimulated]


title: GM_plate4-60 [GM_single_cell_unstimulated]


title: GM_plate4-61 [GM_single_cell_unstimulated]


title: GM_plate4-5 [GM_single_cell_unstimulated]


title: GM_plate4-77 [GM_single_cell_unstimulated]


title: GM_plate4-58 [GM_single_cell_unstimulated]


title: GM_plate4-59 [GM_single_cell_unstimulated]


title: GM_plate4-57 [GM_single_cell_unstimulated]


title: GM_plate4-55 [GM_single_cell_unstimulated]


title: GM_plate4-53 [GM_single_cell_unstimulated]


title: GM_plate4-54 [GM_single_cell_unstimulated]


title: GM_plate4-52 [GM_single_cell_unstimulated]


title: GM_plate4-51 [GM_single_cell_unstimulated]


title: GM_plate4-50 [GM_single_cell_unstimulated]


title: GM_plate4-4 [GM_single_cell_unstimulated]


title: GM_plate4-56 [GM_single_cell_unstimulated]


title: GM_plate4-49 [GM_single_cell_unstimulated]


title: GM_plate4-48 [GM_single_cell_unstimulated]


title: GM_plate4-44 [GM_single_cell_unstimulated]


title: GM_plate4-47 [GM_single_cell_unstimulated]


title: GM_plate4-45 [GM_single_cell_unstimulated]


title: GM_plate4-42 [GM_single_cell_unstimulated]


title: GM_plate4-43 [GM_single_cell_unstimulated]


title: GM_plate4-40 [GM_single_cell_unstimulated]


title: GM_plate4-3 [GM_single_cell_unstimulated]


title: GM_plate4-39 [GM_single_cell_unstimulated]


title: GM_plate4-41 [GM_single_cell_unstimulated]


title: GM_plate4-34 [GM_single_cell_unstimulated]


title: GM_plate4-35 [GM_single_cell_unstimulated]


title: GM_plate4-38 [GM_single_cell_unstimulated]


title: GM_plate4-33 [GM_single_cell_unstimulated]


title: GM_plate4-36 [GM_single_cell_unstimulated]


title: GM_plate4-32 [GM_single_cell_unstimulated]


title: GM_plate4-46 [GM_single_cell_unstimulated]


title: GM_plate4-31 [GM_single_cell_unstimulated]


title: GM_plate4-29 [GM_single_cell_unstimulated]


title: GM_plate4-26 [GM_single_cell_unstimulated]


title: GM_plate4-30 [GM_single_cell_unstimulated]


title: GM_plate4-27 [GM_single_cell_unstimulated]


title: GM_plate4-28 [GM_single_cell_unstimulated]


title: GM_plate4-20 [GM_single_cell_unstimulated]


title: GM_plate4-21 [GM_single_cell_unstimulated]


title: GM_plate4-25 [GM_single_cell_unstimulated]


title: GM_plate4-23 [GM_single_cell_unstimulated]


title: GM_plate4-22 [GM_single_cell_unstimulated]


title: GM_plate4-24 [GM_single_cell_unstimulated]


title: GM_plate4-1 [GM_single_cell_unstimulated]


title: GM_plate4-19 [GM_single_cell_unstimulated]


title: GM_plate4-18 [GM_single_cell_unstimulated]


title: GM_plate4-15 [GM_single_cell_unstimulated]


title: GM_plate4-16 [GM_single_cell_unstimulated]


title: GM_plate4-17 [GM_single_cell_unstimulated]


title: GM_plate4-37 [GM_single_cell_unstimulated]


title: GM_plate4-14 [GM_single_cell_unstimulated]


title: GM_plate4-13 [GM_single_cell_unstimulated]


title: GM_plate4-11 [GM_single_cell_unstimulated]


title: GM_plate4-10 [GM_single_cell_unstimulated]


title: GM_plate4-12 [GM_single_cell_unstimulated]


title: GM_plate3-9 [GM_single_cell_unstimulated]


title: GM_plate3-96 [GM_single_cell_unstimulated]


title: GM_plate3-95 [GM_single_cell_unstimulated]


title: GM_plate3-92 [GM_single_cell_unstimulated]


title: GM_plate3-94 [GM_single_cell_unstimulated]


title: GM_plate3-93 [GM_single_cell_unstimulated]


title: GM_plate3-91 [GM_single_cell_unstimulated]


title: GM_plate3-8 [GM_single_cell_unstimulated]


title: GM_plate3-90 [GM_single_cell_unstimulated]


title: GM_plate3-88 [GM_single_cell_unstimulated]


title: GM_plate3-83 [GM_single_cell_unstimulated]


title: GM_plate3-84 [GM_single_cell_unstimulated]


title: GM_plate3-82 [GM_single_cell_unstimulated]


title: GM_plate3-86 [GM_single_cell_unstimulated]


title: GM_plate3-87 [GM_single_cell_unstimulated]


title: GM_plate3-85 [GM_single_cell_unstimulated]


title: GM_plate3-89 [GM_single_cell_unstimulated]


title: GM_plate3-80 [GM_single_cell_unstimulated]


title: GM_plate3-79 [GM_single_cell_unstimulated]


title: GM_plate3-78 [GM_single_cell_unstimulated]


title: GM_plate3-7 [GM_single_cell_unstimulated]


title: GM_plate3-77 [GM_single_cell_unstimulated]


title: GM_plate4-2 [GM_single_cell_unstimulated]


title: GM_plate3-74 [GM_single_cell_unstimulated]


title: GM_plate4-64 [GM_single_cell_unstimulated]


title: GM_plate3-6 [GM_single_cell_unstimulated]


title: GM_plate3-76 [GM_single_cell_unstimulated]


title: GM_plate3-72 [GM_single_cell_unstimulated]


title: GM_plate3-75 [GM_single_cell_unstimulated]


title: GM_plate3-69 [GM_single_cell_unstimulated]


title: GM_plate3-68 [GM_single_cell_unstimulated]


title: GM_plate3-67 [GM_single_cell_unstimulated]


title: GM_plate3-73 [GM_single_cell_unstimulated]


title: GM_plate3-63 [GM_single_cell_unstimulated]


title: GM_plate3-71 [GM_single_cell_unstimulated]


title: GM_plate3-5 [GM_single_cell_unstimulated]


title: GM_plate3-57 [GM_single_cell_unstimulated]


title: GM_plate3-56 [GM_single_cell_unstimulated]


title: GM_plate3-64 [GM_single_cell_unstimulated]


title: GM_plate3-65 [GM_single_cell_unstimulated]


title: GM_plate3-60 [GM_single_cell_unstimulated]


title: GM_plate3-55 [GM_single_cell_unstimulated]


title: GM_plate3-70 [GM_single_cell_unstimulated]


title: GM_plate3-61 [GM_single_cell_unstimulated]


title: GM_plate3-51 [GM_single_cell_unstimulated]


title: GM_plate3-53 [GM_single_cell_unstimulated]


title: GM_plate3-4 [GM_single_cell_unstimulated]


title: GM_plate3-62 [GM_single_cell_unstimulated]


title: GM_plate3-50 [GM_single_cell_unstimulated]


title: GM_plate3-59 [GM_single_cell_unstimulated]


title: GM_plate3-66 [GM_single_cell_unstimulated]


title: GM_plate3-81 [GM_single_cell_unstimulated]


title: GM_plate3-48 [GM_single_cell_unstimulated]


title: GM_plate3-52 [GM_single_cell_unstimulated]


title: GM_plate3-54 [GM_single_cell_unstimulated]


title: GM_plate3-49 [GM_single_cell_unstimulated]


title: GM_plate3-43 [GM_single_cell_unstimulated]


title: GM_plate3-58 [GM_single_cell_unstimulated]


title: GM_plate3-45 [GM_single_cell_unstimulated]


title: GM_plate3-44 [GM_single_cell_unstimulated]


title: GM_plate3-42 [GM_single_cell_unstimulated]


title: GM_plate3-41 [GM_single_cell_unstimulated]


title: GM_plate3-40 [GM_single_cell_unstimulated]


title: GM_plate3-3 [GM_single_cell_unstimulated]


title: GM_plate3-39 [GM_single_cell_unstimulated]


title: GM_plate3-38 [GM_single_cell_unstimulated]


title: GM_plate3-35 [GM_single_cell_unstimulated]


title: GM_plate3-37 [GM_single_cell_unstimulated]


title: GM_plate3-36 [GM_single_cell_unstimulated]


title: GM_plate3-33 [GM_single_cell_unstimulated]


title: GM_plate3-32 [GM_single_cell_unstimulated]


title: GM_plate3-31 [GM_single_cell_unstimulated]


title: GM_plate3-30 [GM_single_cell_unstimulated]


title: GM_plate3-47 [GM_single_cell_unstimulated]


title: GM_plate3-46 [GM_single_cell_unstimulated]


title: GM_plate3-28 [GM_single_cell_unstimulated]


title: GM_plate3-27 [GM_single_cell_unstimulated]


title: GM_plate3-2 [GM_single_cell_unstimulated]


title: GM_plate3-26 [GM_single_cell_unstimulated]


title: GM_plate3-25 [GM_single_cell_unstimulated]


title: GM_plate3-23 [GM_single_cell_unstimulated]


title: GM_plate3-21 [GM_single_cell_unstimulated]


title: GM_plate3-20 [GM_single_cell_unstimulated]


title: GM_plate3-19 [GM_single_cell_unstimulated]


title: GM_plate3-1 [GM_single_cell_unstimulated]


title: GM_plate3-18 [GM_single_cell_unstimulated]


title: GM_plate3-24 [GM_single_cell_unstimulated]


title: GM_plate3-17 [GM_single_cell_unstimulated]


title: GM_plate3-16 [GM_single_cell_unstimulated]


title: GM_plate3-15 [GM_single_cell_unstimulated]


title: GM_plate3-14 [GM_single_cell_unstimulated]


title: GM_plate3-34 [GM_single_cell_unstimulated]


title: GM_plate3-12 [GM_single_cell_unstimulated]


title: GM_plate3-13 [GM_single_cell_unstimulated]


title: GM_plate3-29 [GM_single_cell_unstimulated]


title: GM_plate3-11 [GM_single_cell_unstimulated]


title: GM_plate3-10 [GM_single_cell_unstimulated]


title: GM_plate3-22 [GM_single_cell_unstimulated]


title: GM_plate2-96 [GM_single_cell_unstimulated]


title: GM_plate2-95 [GM_single_cell_unstimulated]


title: GM_plate2-92 [GM_single_cell_unstimulated]


title: GM_plate2-9 [GM_single_cell_unstimulated]


title: GM_plate2-91 [GM_single_cell_unstimulated]


title: GM_plate2-86 [GM_single_cell_unstimulated]


title: GM_plate2-88 [GM_single_cell_unstimulated]


title: GM_plate2-89 [GM_single_cell_unstimulated]


title: GM_plate2-85 [GM_single_cell_unstimulated]


title: GM_plate2-81 [GM_single_cell_unstimulated]


title: GM_plate2-82 [GM_single_cell_unstimulated]


title: GM_plate2-8 [GM_single_cell_unstimulated]


title: GM_plate2-87 [GM_single_cell_unstimulated]


title: GM_plate2-83 [GM_single_cell_unstimulated]


title: GM_plate2-90 [GM_single_cell_unstimulated]


title: GM_plate2-7 [GM_single_cell_unstimulated]


title: GM_plate2-84 [GM_single_cell_unstimulated]


title: GM_plate2-80 [GM_single_cell_unstimulated]


title: GM_plate2-79 [GM_single_cell_unstimulated]


title: GM_plate2-74 [GM_single_cell_unstimulated]


title: GM_plate2-76 [GM_single_cell_unstimulated]


title: GM_plate2-94 [GM_single_cell_unstimulated]


title: GM_plate2-72 [GM_single_cell_unstimulated]


title: GM_plate2-93 [GM_single_cell_unstimulated]


title: GM_plate2-73 [GM_single_cell_unstimulated]


title: GM_plate2-78 [GM_single_cell_unstimulated]


title: GM_plate2-71 [GM_single_cell_unstimulated]


title: GM_plate2-75 [GM_single_cell_unstimulated]


title: GM_plate2-70 [GM_single_cell_unstimulated]


title: GM_plate2-6 [GM_single_cell_unstimulated]


title: GM_plate2-69 [GM_single_cell_unstimulated]


title: GM_plate2-68 [GM_single_cell_unstimulated]


title: GM_plate2-64 [GM_single_cell_unstimulated]


title: GM_plate2-63 [GM_single_cell_unstimulated]


title: GM_plate2-77 [GM_single_cell_unstimulated]


title: GM_plate2-65 [GM_single_cell_unstimulated]


title: GM_plate2-62 [GM_single_cell_unstimulated]


title: GM_plate2-55 [GM_single_cell_unstimulated]


title: GM_plate2-57 [GM_single_cell_unstimulated]


title: GM_plate2-5 [GM_single_cell_unstimulated]


title: GM_plate2-56 [GM_single_cell_unstimulated]


title: GM_plate2-66 [GM_single_cell_unstimulated]


title: GM_plate2-54 [GM_single_cell_unstimulated]


title: GM_plate2-60 [GM_single_cell_unstimulated]


title: GM_plate2-53 [GM_single_cell_unstimulated]


title: GM_plate2-59 [GM_single_cell_unstimulated]


title: GM_plate2-61 [GM_single_cell_unstimulated]


title: GM_plate2-52 [GM_single_cell_unstimulated]


title: GM_plate2-50 [GM_single_cell_unstimulated]


title: GM_plate2-49 [GM_single_cell_unstimulated]


title: GM_plate2-48 [GM_single_cell_unstimulated]


title: GM_plate2-47 [GM_single_cell_unstimulated]


title: GM_plate2-51 [GM_single_cell_unstimulated]


title: GM_plate2-43 [GM_single_cell_unstimulated]


title: GM_plate2-44 [GM_single_cell_unstimulated]


title: GM_plate2-67 [GM_single_cell_unstimulated]


title: GM_plate2-58 [GM_single_cell_unstimulated]


title: GM_plate2-41 [GM_single_cell_unstimulated]


title: GM_plate2-38 [GM_single_cell_unstimulated]


title: GM_plate2-3 [GM_single_cell_unstimulated]


title: GM_plate2-40 [GM_single_cell_unstimulated]


title: GM_plate2-39 [GM_single_cell_unstimulated]


title: GM_plate2-42 [GM_single_cell_unstimulated]


title: GM_plate2-36 [GM_single_cell_unstimulated]


title: GM_plate2-34 [GM_single_cell_unstimulated]


title: GM_plate2-31 [GM_single_cell_unstimulated]


title: GM_plate2-33 [GM_single_cell_unstimulated]


title: GM_plate2-2 [GM_single_cell_unstimulated]


title: GM_plate2-4 [GM_single_cell_unstimulated]


title: GM_plate2-32 [GM_single_cell_unstimulated]


title: GM_plate2-30 [GM_single_cell_unstimulated]


title: GM_plate2-29 [GM_single_cell_unstimulated]


title: GM_plate2-26 [GM_single_cell_unstimulated]


title: GM_plate2-27 [GM_single_cell_unstimulated]


title: GM_plate2-25 [GM_single_cell_unstimulated]


title: GM_plate2-37 [GM_single_cell_unstimulated]


title: GM_plate2-24 [GM_single_cell_unstimulated]


title: GM_plate2-46 [GM_single_cell_unstimulated]


title: GM_plate2-45 [GM_single_cell_unstimulated]


title: GM_plate2-28 [GM_single_cell_unstimulated]


title: GM_plate2-23 [GM_single_cell_unstimulated]


title: GM_plate2-22 [GM_single_cell_unstimulated]


title: GM_plate2-1 [GM_single_cell_unstimulated]


title: GM_plate2-20 [GM_single_cell_unstimulated]


title: GM_plate2-19 [GM_single_cell_unstimulated]


title: GM_plate2-35 [GM_single_cell_unstimulated]


title: GM_plate2-18 [GM_single_cell_unstimulated]


title: GM_plate2-17 [GM_single_cell_unstimulated]


title: GM_plate2-14 [GM_single_cell_unstimulated]


title: GM_plate2-15 [GM_single_cell_unstimulated]


title: GM_plate2-16 [GM_single_cell_unstimulated]


title: GM_plate2-13 [GM_single_cell_unstimulated]


title: GM_plate1-96 [GM_single_cell_unstimulated]


title: GM_plate2-11 [GM_single_cell_unstimulated]


title: GM_plate1-93 [GM_single_cell_unstimulated]


title: GM_plate1-92 [GM_single_cell_unstimulated]


title: GM_plate1-89 [GM_single_cell_unstimulated]


title: GM_plate1-88 [GM_single_cell_unstimulated]


title: GM_plate2-10 [GM_single_cell_unstimulated]


title: GM_plate1-86 [GM_single_cell_unstimulated]


title: GM_plate1-85 [GM_single_cell_unstimulated]


title: GM_plate1-91 [GM_single_cell_unstimulated]


title: GM_plate1-84 [GM_single_cell_unstimulated]


title: GM_plate1-8 [GM_single_cell_unstimulated]


title: GM_plate2-21 [GM_single_cell_unstimulated]


title: GM_plate1-94 [GM_single_cell_unstimulated]


title: GM_plate1-95 [GM_single_cell_unstimulated]


title: GM_plate1-83 [GM_single_cell_unstimulated]


title: GM_plate1-82 [GM_single_cell_unstimulated]


title: GM_plate1-87 [GM_single_cell_unstimulated]


title: GM_plate1-81 [GM_single_cell_unstimulated]


title: GM_plate2-12 [GM_single_cell_unstimulated]


title: GM_plate1-80 [GM_single_cell_unstimulated]


title: GM_plate1-7 [GM_single_cell_unstimulated]


title: GM_plate1-79 [GM_single_cell_unstimulated]


title: GM_plate1-9 [GM_single_cell_unstimulated]


title: GM_plate1-78 [GM_single_cell_unstimulated]


title: GM_plate1-77 [GM_single_cell_unstimulated]


title: GM_plate1-76 [GM_single_cell_unstimulated]


title: GM_plate1-75 [GM_single_cell_unstimulated]


title: GM_plate1-90 [GM_single_cell_unstimulated]


title: GM_plate1-72 [GM_single_cell_unstimulated]


title: GM_plate1-70 [GM_single_cell_unstimulated]


title: GM_plate1-68 [GM_single_cell_unstimulated]


title: GM_plate1-69 [GM_single_cell_unstimulated]


title: GM_plate1-67 [GM_single_cell_unstimulated]


title: GM_plate1-65 [GM_single_cell_unstimulated]


title: GM_plate1-66 [GM_single_cell_unstimulated]


title: GM_plate1-63 [GM_single_cell_unstimulated]


title: GM_plate1-62 [GM_single_cell_unstimulated]


title: GM_plate1-64 [GM_single_cell_unstimulated]


title: GM_plate1-60 [GM_single_cell_unstimulated]


title: GM_plate1-61 [GM_single_cell_unstimulated]


title: GM_plate1-59 [GM_single_cell_unstimulated]


title: GM_plate1-5 [GM_single_cell_unstimulated]


title: GM_plate1-58 [GM_single_cell_unstimulated]


title: GM_plate1-56 [GM_single_cell_unstimulated]


title: GM_plate1-57 [GM_single_cell_unstimulated]


title: GM_plate1-55 [GM_single_cell_unstimulated]


title: GM_plate1-53 [GM_single_cell_unstimulated]


title: GM_plate1-74 [GM_single_cell_unstimulated]


title: GM_plate1-52 [GM_single_cell_unstimulated]


title: GM_plate1-4 [GM_single_cell_unstimulated]


title: GM_plate1-73 [GM_single_cell_unstimulated]


title: GM_plate1-51 [GM_single_cell_unstimulated]


title: GM_plate1-49 [GM_single_cell_unstimulated]


title: GM_plate1-50 [GM_single_cell_unstimulated]


title: GM_plate1-71 [GM_single_cell_unstimulated]


title: GM_plate1-54 [GM_single_cell_unstimulated]


title: GM_plate1-48 [GM_single_cell_unstimulated]


title: GM_plate1-6 [GM_single_cell_unstimulated]


title: GM_plate1-46 [GM_single_cell_unstimulated]


title: GM_plate1-44 [GM_single_cell_unstimulated]


title: GM_plate1-43 [GM_single_cell_unstimulated]


title: GM_plate1-40 [GM_single_cell_unstimulated]


title: GM_plate1-42 [GM_single_cell_unstimulated]


title: GM_plate1-41 [GM_single_cell_unstimulated]


title: GM_plate1-3 [GM_single_cell_unstimulated]


title: GM_plate1-38 [GM_single_cell_unstimulated]


title: GM_plate1-37 [GM_single_cell_unstimulated]


title: GM_plate1-36 [GM_single_cell_unstimulated]


title: GM_plate1-32 [GM_single_cell_unstimulated]


title: GM_plate1-34 [GM_single_cell_unstimulated]


title: GM_plate1-30 [GM_single_cell_unstimulated]


title: GM_plate1-35 [GM_single_cell_unstimulated]


title: GM_plate1-31 [GM_single_cell_unstimulated]


title: GM_plate1-2 [GM_single_cell_unstimulated]


title: GM_plate1-33 [GM_single_cell_unstimulated]


title: GM_plate1-29 [GM_single_cell_unstimulated]


title: GM_plate1-28 [GM_single_cell_unstimulated]


title: GM_plate1-27 [GM_single_cell_unstimulated]


title: GM_plate1-22 [GM_single_cell_unstimulated]


title: GM_plate1-21 [GM_single_cell_unstimulated]


title: GM_plate1-24 [GM_single_cell_unstimulated]


title: GM_plate1-26 [GM_single_cell_unstimulated]


title: GM_plate1-23 [GM_single_cell_unstimulated]


title: GM_plate1-47 [GM_single_cell_unstimulated]


title: GM_plate1-20 [GM_single_cell_unstimulated]


title: GM_plate1-39 [GM_single_cell_unstimulated]


title: GM_plate1-45 [GM_single_cell_unstimulated]


title: GM_plate1-1 [GM_single_cell_unstimulated]


title: GM_plate1-19 [GM_single_cell_unstimulated]


title: GM_plate1-18 [GM_single_cell_unstimulated]


title: GM_plate1-17 [GM_single_cell_unstimulated]


title: GM_plate1-16 [GM_single_cell_unstimulated]


title: GM_plate1-12 [GM_single_cell_unstimulated]


title: GM_plate1-14 [GM_single_cell_unstimulated]


title: GM_plate1-13 [GM_single_cell_unstimulated]


title: GM_plate1-11 [GM_single_cell_unstimulated]


title: GM_plate1-25 [GM_single_cell_unstimulated]


title: GM_plate1-10 [GM_single_cell_unstimulated]


title: GM_plate2-96

description: GM12878 cells were fixed, permeabilized and transposed, then sorted into single wells of a 96 well plate for MI-ATAC

extract_protocol: single cell MI-ATAC of GM12878 cells. Single cells were first fixed, then  lysed, followed by  transposition. The transposition reaction is the same as for standard ATAC-seq except with 0.05% Igepal CA-630 in the lysis buffer. After 30 min transposition, at 37 degrees, the reaction was quenched using 40 mM EDTA, then cells were centrifuged and supernatant was discarded. 20 µl of reverse crosslinking solution was added to the cell pellet (with final concentration of 5 ng/ml proteinase K).  Reverse crosslinking was performed 65 degree overnight and inactivated by a 10 min incubation at 80 degrees the next day. The 25 µl PCR master mix (NEB, M0541S) and 5 µl of two unique primer combinations were directly added to the reverse crosslinking mixture and PCR was performed as previous described. The PCR product was purified with Qiagen MinElute purification kit and eluted in 20 µl Qiagen EB elution buffer


title: GM_plate2-92


title: GM_plate2-91


title: GM_plate2-93


title: GM_plate2-95


title: GM_plate2-8


title: GM_plate2-88


title: GM_plate2-90


title: GM_plate2-87


title: GM_plate2-94


title: GM_plate2-86


title: GM_plate2-84


title: GM_plate2-83


title: GM_plate2-89


title: GM_plate2-81


title: GM_plate2-7


title: GM_plate2-82


title: GM_plate2-80


title: GM_plate1-15 [GM_single_cell_unstimulated]


title: GM_plate2-79


title: GM_plate2-78


title: GM_plate2-77


title: GM_plate2-76


title: GM_plate2-75


title: GM_plate2-72


title: GM_plate2-71


title: GM_plate2-70


title: GM_plate2-73


title: GM_plate2-74


title: GM_plate2-85


title: GM_plate2-69


title: GM_plate2-68


title: GM_plate2-6


title: GM_plate2-66


title: GM_plate2-65


title: GM_plate2-62


title: GM_plate2-64


title: GM_plate2-67


title: GM_plate2-61


title: GM_plate2-5


title: GM_plate2-63


title: GM_plate2-60


title: GM_plate2-56


title: GM_plate2-57


title: GM_plate2-55


title: GM_plate2-54


title: GM_plate2-58


title: GM_plate2-59


title: GM_plate2-53


title: GM_plate2-50


title: GM_plate2-4


title: GM_plate2-49


title: GM_plate2-52


title: GM_plate2-51


title: GM_plate2-48


title: GM_plate2-47


title: GM_plate2-46


title: GM_plate2-41


title: GM_plate2-40


title: GM_plate2-42


title: GM_plate2-44


title: GM_plate2-45


title: GM_plate2-43


title: GM_plate2-38


title: GM_plate2-36


title: GM_plate2-34


title: GM_plate2-32


title: GM_plate2-35


title: GM_plate2-33


title: GM_plate2-3


title: GM_plate2-37


title: GM_plate2-29


title: GM_plate2-28


title: GM_plate2-26


title: GM_plate2-30


title: GM_plate2-27


title: GM_plate2-2


title: GM_plate2-25


title: GM_plate2-24


title: GM_plate2-22


title: GM_plate2-23


title: GM_plate2-21


title: GM_plate2-19


title: GM_plate2-1


title: GM_plate2-20


title: GM_plate2-39


title: GM_plate2-18


title: GM_plate2-17


title: GM_plate2-13


title: GM_plate2-16


title: GM_plate2-14


title: GM_plate2-15


title: GM_plate2-12


title: GM_plate2-11


title: GM_plate2-31


title: GM_plate1-9


title: GM_plate2-10


title: GM_plate1-95


title: GM_plate1-93


title: GM_plate1-96


title: GM_plate1-91


title: GM_plate1-90


title: GM_plate1-8


title: GM_plate1-92


title: GM_plate1-89


title: GM_plate1-88


title: GM_plate1-87


title: GM_plate1-83


title: GM_plate1-86


title: GM_plate1-81


title: GM_plate1-7


title: GM_plate1-80


title: GM_plate1-79


title: GM_plate1-77


title: GM_plate1-78


title: GM_plate1-94


title: GM_plate1-76


title: GM_plate1-74


title: GM_plate1-75


title: GM_plate1-73


title: GM_plate1-6


title: GM_plate1-70


title: GM_plate1-71


title: GM_plate1-68


title: GM_plate1-72


title: GM_plate1-85


title: GM_plate1-69


title: GM_plate1-65


title: GM_plate1-64


title: GM_plate1-63


title: GM_plate1-84


title: GM_plate1-61


title: GM_plate1-62


title: GM_plate1-66


title: GM_plate1-5


title: GM_plate1-82


title: GM_plate1-58


title: GM_plate1-57


title: GM_plate1-53


title: GM_plate1-54


title: GM_plate1-55


title: GM_plate1-56


title: GM_plate1-67


title: GM_plate1-52


title: GM_plate1-48


title: GM_plate1-49


title: GM_plate1-51


title: GM_plate1-46


title: GM_plate1-4


title: GM_plate1-45


title: GM_plate1-50


title: GM_plate1-41


title: GM_plate1-3


title: GM_plate1-42


title: GM_plate1-60


title: GM_plate1-59


title: GM_plate1-40


title: GM_plate1-38


title: GM_plate1-36


title: GM_plate1-44


title: GM_plate1-43


title: GM_plate1-37


title: GM_plate1-39


title: GM_plate1-35


title: GM_plate1-34


title: GM_plate1-33


title: GM_plate1-32


title: GM_plate1-31


title: GM_plate1-47


title: GM_plate1-2


title: GM_plate1-30


title: GM_plate1-27


title: GM_plate1-23


title: GM_plate1-24


title: GM_plate1-25


title: GM_plate1-22


title: GM_plate1-28


title: GM_plate1-20


title: GM_plate1-21


title: GM_plate1-16


title: GM_plate1-17


title: GM_plate1-18


title: GM_plate1-1


title: GM_plate1-19


title: GM_plate1-13


title: GM_plate1-14


title: GM_plate1-15


title: GM_plate1-10


title: bulk_fixed ATACseq_new buffer rep2

description: No antibody used

data_processing:
Paired-end reads were trimmed for Illumina adapter sequences and transposase sequences using an in-house script and mapped to hg19 using Bowtie2 v2.1.0 with parameters –very sensitive. Reads were subsequently filtered for alignment quality of > Q30 and were required to be properly paired. Duplicate reads were removed with Picard (http://picard.sourceforge.net) v1.79. Reads mapping to the mitochondria or chromosome Y were removed and not considered. Peak calling was performed by MACS2 narrow peak mode with parameters –q 0.01 –nomodel –shift 0. Peaks were filtered using the consensus excludable ENCODE blacklist ( http://hgdownload.cse.ucsc.edu/goldenPath/hg19/encodeDCC/wgEncodeMapability/) and a custom blacklist designed to remove high-signal-causing repeats and mitochondrial homologues. Overlapping peaks from all samples were merged together to a consensus peak list, and normalized using DESeq. The number of unique and properly paired reads mapped to each peak for each individual samples was quantified to calculate the Pearson Correlation
Genome_build: hg19
Supplementary_files_format_and_content: narrow.peak files were generated by MACS2 narrow peak mode with parameters –q 0.01 –nomodel –shift 0. Replicates were merged. Then peaks were filtered using the consensus excludable ENCODE blacklist.

source_name: reverse crosslinking using nonSDS containing buffer

extract_protocol: bulk MI-ATAC development. GM cells were fixed in 1 % PFA. The transposition reaction is the same as for standard ATAC-seq except with 0.05% Igepal CA-630 in the lysis buffer. After 30 min transposition, at 37 degrees, the reaction was quenched using 40 mM EDTA, then cells were centrifuged and supernatant was discarded. 20 µl of diverse reverse crosslinking solutions was added to the cell pellet (with final concentration of 5 ng/ml proteinase K).  Reverse crosslinking was performed 65 degree overnight and inactivated by a 10 min incubation at 80 degrees the next day. The 25 µl PCR master mix (NEB, M0541S) and 5 µl of two unique primer combinations were either added directly to the reverse crosslinking mixture  or after DNA purification. Then barcoding PCR was performed as previous described. The PCR product was purified with Qiagen MinElute purification kit and eluted in 20 µl Qiagen EB elution buffer

value: GM12878 cells


title: GM_plate1-11


title: GM_plate1-29


title: bulk_fixed ATACseq_rep2

description: No antibody used

source_name: reverse crosslinking using SDS containing buffer


title: GM_plate1-26


title: bulk_MI-ATAC-seq_rep2

description: No antibody used

source_name: reverse crosslinking using non SDS containing buffer and without DNA purification


title: bulk_fixed ATACseq_rep1

description: No antibody used


title: C57BL/6 Gut Microbiome with High Fat Diet, Mouse3

value: tRNA


title: C57BL/6 Gut Microbiome with High Fat Diet, Mouse1


title: C57BL/6 Gut Microbiome with High Fat Diet, Mouse2


title: C57BL/6 Gut Microbiome with Low Fat Diet, Mouse3


title: C57BL/6 Gut Microbiome with Low Fat Diet, Mouse2


title: C57BL/6 Gut Microbiome with Low Fat Diet, Mouse1


title: Tumor-plate9-94 [EpCam and CD45]

description: Freshly isolated cells from a breast tumor were fixed, permeabilized and stained against EpCam and CD45,  then sorted into single wells of a 96 well plate for MI-ATAC

data_processing:
Paired-end reads were trimmed for Illumina adapter sequences and transposase sequences using an in-house script and mapped to mm9 using Bowtie2 v2.1.0 with parameters –very sensitive. Reads were subsequently filtered for alignment quality of > Q30 and were required to be properly paired. Duplicate reads were removed with Picard (http://picard.sourceforge.net) v1.79. Reads mapping to the mitochondria or chromosome Y were removed and not considered. Peak calling was performed by MACS2 with parameters --call-summits –q 0.05 –nomodel –shift 0. Peaks were filtered using the consensus excludable ENCODE blacklist ( http://hgdownload.cse.ucsc.edu/goldenPath/hg19/encodeDCC/wgEncodeMapability/) and a custom blacklist designed to remove high-signal-causing repeats and mitochondrial homologues. Overlapping peaks from all samples were merged together to a consensus peak list. Then peak region was extended to 500 bp around the summit.
Genome_build: mm9
Supplementary_files_format_and_content: Peak calling was performed by MACS2 with parameters --call-summits –q 0.05 –nomodel –shift 0. Peaks were filtered using the consensus excludable ENCODE blacklist ( http://hgdownload.cse.ucsc.edu/goldenPath/mm9/encodeDCC/wgEncodeMapability/) and a custom blacklist designed to remove high-signal-causing repeats and mitochondrial homologues. Overlapping peaks from all samples were merged together to a consensus peak list. Then peak region was extended to 500 bp around the summit.

source_name: mouse breast cancer

extract_protocol: single cell MI-ATAC of primary mouse breast cancer cells. Single cells were first fixed, then  lysed, followed by staining for respective antibodies and transposition. The transposition reaction is the same as for standard ATAC-seq except with 0.05% Igepal CA-630 in the lysis buffer. After 30 min transposition, at 37 degrees, the reaction was quenched using 40 mM EDTA, then cells were centrifuged and supernatant was discarded. 20 µl of reverse crosslinking solution was added to the cell pellet (with final concentration of 5 ng/ml proteinase K).  Reverse crosslinking was performed 65 degree overnight and inactivated by a 10 min incubation at 80 degrees the next day. The 25 µl PCR master mix (NEB, M0541S) and 5 µl of two unique primer combinations were directly added to the reverse crosslinking mixture and PCR was performed as previous described. The PCR product was purified with Qiagen MinElute purification kit and eluted in 20 µl Qiagen EB elution buffer

value: single cells were isolated from PyMT mouse breast tumor tissue

value: EpCam and CD45


title: Tumor-plate9-91 [EpCam and CD45]


title: Tumor-plate9-96 [EpCam and CD45]


title: Tumor-plate9-9 [EpCam and CD45]


title: Tumor-plate9-88 [EpCam and CD45]


title: Tumor-plate9-93 [EpCam and CD45]


title: GM_plate1-12


title: Tumor-plate9-85 [EpCam and CD45]


title: bulk_fixed ATACseq_new buffer rep1

description: No antibody used


title: Tumor-plate9-86 [EpCam and CD45]


title: Tumor-plate9-90 [EpCam and CD45]


title: Tumor-plate9-84 [EpCam and CD45]


title: Tumor-plate9-82 [EpCam and CD45]


title: Tumor-plate9-89 [EpCam and CD45]


title: Tumor-plate9-83 [EpCam and CD45]


title: Tumor-plate9-81 [EpCam and CD45]


title: Tumor-plate9-80 [EpCam and CD45]


title: bulk_MI-ATAC-seq_rep1

description: No antibody used


title: Tumor-plate9-87 [EpCam and CD45]


title: Tumor-plate9-8 [EpCam and CD45]


title: Tumor-plate9-79 [EpCam and CD45]


title: Tumor-plate9-95 [EpCam and CD45]


title: Tumor-plate9-77 [EpCam and CD45]


title: Tumor-plate9-76 [EpCam and CD45]


title: Tumor-plate9-78 [EpCam and CD45]


title: Tumor-plate9-92 [EpCam and CD45]


title: Tumor-plate9-72 [EpCam and CD45]


title: Tumor-plate9-73 [EpCam and CD45]


title: Tumor-plate9-74 [EpCam and CD45]


title: Tumor-plate9-71 [EpCam and CD45]


title: Tumor-plate9-70 [EpCam and CD45]


title: Tumor-plate9-64 [EpCam and CD45]


title: Tumor-plate9-66 [EpCam and CD45]


title: Tumor-plate9-68 [EpCam and CD45]


title: Tumor-plate9-65 [EpCam and CD45]


title: Tumor-plate9-62 [EpCam and CD45]


title: Tumor-plate9-61 [EpCam and CD45]


title: Tumor-plate9-63 [EpCam and CD45]


title: Tumor-plate9-57 [EpCam and CD45]


title: Tumor-plate9-6 [EpCam and CD45]


title: Tumor-plate9-60 [EpCam and CD45]


title: Tumor-plate9-58 [EpCam and CD45]


title: Tumor-plate9-56 [EpCam and CD45]


title: Tumor-plate9-55 [EpCam and CD45]


title: Tumor-plate9-59 [EpCam and CD45]


title: Tumor-plate9-54 [EpCam and CD45]


title: Tumor-plate9-53 [EpCam and CD45]


title: Tumor-plate9-75 [EpCam and CD45]


title: Tumor-plate9-52 [EpCam and CD45]


title: Tumor-plate9-50 [EpCam and CD45]


title: Tumor-plate9-49 [EpCam and CD45]


title: Tumor-plate9-47 [EpCam and CD45]


title: Tumor-plate9-51 [EpCam and CD45]


title: Tumor-plate9-7 [EpCam and CD45]


title: Tumor-plate9-5 [EpCam and CD45]


title: Tumor-plate9-46 [EpCam and CD45]


title: Tumor-plate9-69 [EpCam and CD45]


title: Tumor-plate9-45 [EpCam and CD45]


title: Tumor-plate9-44 [EpCam and CD45]


title: Tumor-plate9-67 [EpCam and CD45]


title: Tumor-plate9-43 [EpCam and CD45]


title: Tumor-plate9-42 [EpCam and CD45]


title: Tumor-plate9-41 [EpCam and CD45]


title: Tumor-plate9-39 [EpCam and CD45]


title: Tumor-plate9-40 [EpCam and CD45]


title: Tumor-plate9-4 [EpCam and CD45]


title: Tumor-plate9-37 [EpCam and CD45]


title: Tumor-plate9-33 [EpCam and CD45]


title: Tumor-plate9-34 [EpCam and CD45]


title: Tumor-plate9-35 [EpCam and CD45]


title: Tumor-plate9-36 [EpCam and CD45]


title: Tumor-plate9-31 [EpCam and CD45]


title: Tumor-plate9-29 [EpCam and CD45]


title: Tumor-plate9-48 [EpCam and CD45]


title: Tumor-plate9-28 [EpCam and CD45]


title: Tumor-plate9-27 [EpCam and CD45]


title: Tumor-plate9-25 [EpCam and CD45]


title: Tumor-plate9-26 [EpCam and CD45]


title: Tumor-plate9-23 [EpCam and CD45]


title: Tumor-plate9-21 [EpCam and CD45]


title: Tumor-plate9-24 [EpCam and CD45]


title: Tumor-plate9-22 [EpCam and CD45]


title: Tumor-plate9-3 [EpCam and CD45]


title: Tumor-plate9-20 [EpCam and CD45]


title: Tumor-plate9-19 [EpCam and CD45]


title: Tumor-plate9-2 [EpCam and CD45]


title: Tumor-plate9-17 [EpCam and CD45]


title: Tumor-plate9-32 [EpCam and CD45]


title: Tumor-plate9-18 [EpCam and CD45]


title: Tumor-plate9-12 [EpCam and CD45]


title: Tumor-plate9-16 [EpCam and CD45]


title: Tumor-plate9-30 [EpCam and CD45]


title: Tumor-plate9-11 [EpCam and CD45]


title: Tumor-plate9-38 [EpCam and CD45]


title: Tumor-plate9-10 [EpCam and CD45]


title: Tumor-plate9-1 [EpCam and CD45]


title: Tumor-plate8-95 [EpCam and CD45]


title: Tumor-plate8-96 [EpCam and CD45]


title: Tumor-plate8-94 [EpCam and CD45]


title: Tumor-plate8-93 [EpCam and CD45]


title: Tumor-plate8-92 [EpCam and CD45]


title: Tumor-plate8-91 [EpCam and CD45]


title: Tumor-plate8-9 [EpCam and CD45]


title: Tumor-plate8-90 [EpCam and CD45]


title: Tumor-plate8-88 [EpCam and CD45]


title: Tumor-plate8-89 [EpCam and CD45]


title: Tumor-plate8-87 [EpCam and CD45]


title: Tumor-plate8-86 [EpCam and CD45]


title: Tumor-plate8-84 [EpCam and CD45]


title: Tumor-plate8-83 [EpCam and CD45]


title: Tumor-plate8-82 [EpCam and CD45]


title: Tumor-plate8-81 [EpCam and CD45]


title: Tumor-plate9-13 [EpCam and CD45]


title: Tumor-plate9-14 [EpCam and CD45]


title: Tumor-plate8-80 [EpCam and CD45]


title: Tumor-plate8-8 [EpCam and CD45]


title: Tumor-plate9-15 [EpCam and CD45]


title: Tumor-plate8-79 [EpCam and CD45]


title: Tumor-plate8-78 [EpCam and CD45]


title: Tumor-plate8-76 [EpCam and CD45]


title: Tumor-plate8-74 [EpCam and CD45]


title: Tumor-plate8-75 [EpCam and CD45]


title: Tumor-plate8-72 [EpCam and CD45]


title: Tumor-plate8-73 [EpCam and CD45]


title: Tumor-plate8-69 [EpCam and CD45]


title: Tumor-plate8-7 [EpCam and CD45]


title: Tumor-plate8-67 [EpCam and CD45]


title: Tumor-plate8-68 [EpCam and CD45]


title: Tumor-plate8-77 [EpCam and CD45]


title: Tumor-plate8-66 [EpCam and CD45]


title: Tumor-plate8-85 [EpCam and CD45]


title: Tumor-plate8-64 [EpCam and CD45]


title: Tumor-plate8-63 [EpCam and CD45]


title: Tumor-plate8-62 [EpCam and CD45]


title: Tumor-plate8-61 [EpCam and CD45]


title: Tumor-plate8-60 [EpCam and CD45]


title: Tumor-plate8-6 [EpCam and CD45]


title: Tumor-plate8-56 [EpCam and CD45]


title: Tumor-plate8-57 [EpCam and CD45]


title: Tumor-plate8-59 [EpCam and CD45]


title: Tumor-plate8-58 [EpCam and CD45]


title: Tumor-plate8-54 [EpCam and CD45]


title: Tumor-plate8-70 [EpCam and CD45]


title: Tumor-plate8-53 [EpCam and CD45]


title: Tumor-plate8-55 [EpCam and CD45]


title: Tumor-plate8-71 [EpCam and CD45]


title: Tumor-plate8-52 [EpCam and CD45]


title: Tumor-plate8-48 [EpCam and CD45]


title: Tumor-plate8-49 [EpCam and CD45]


title: Tumor-plate8-47 [EpCam and CD45]


title: Tumor-plate8-51 [EpCam and CD45]


title: Tumor-plate8-50 [EpCam and CD45]


title: Tumor-plate8-44 [EpCam and CD45]


title: Tumor-plate8-46 [EpCam and CD45]


title: Tumor-plate8-5 [EpCam and CD45]


title: Tumor-plate8-43 [EpCam and CD45]


title: Tumor-plate8-65 [EpCam and CD45]


title: Tumor-plate8-42 [EpCam and CD45]


title: Tumor-plate8-41 [EpCam and CD45]


title: Tumor-plate8-40 [EpCam and CD45]


title: Tumor-plate8-45 [EpCam and CD45]


title: Tumor-plate8-4 [EpCam and CD45]


title: Tumor-plate8-39 [EpCam and CD45]


title: Tumor-plate8-38 [EpCam and CD45]


title: Tumor-plate8-36 [EpCam and CD45]


title: Tumor-plate8-35 [EpCam and CD45]


title: Tumor-plate8-34 [EpCam and CD45]


title: Tumor-plate8-33 [EpCam and CD45]


title: Tumor-plate8-32 [EpCam and CD45]


title: Tumor-plate8-30 [EpCam and CD45]


title: Tumor-plate8-3 [EpCam and CD45]


title: Tumor-plate8-28 [EpCam and CD45]


title: Tumor-plate8-31 [EpCam and CD45]


title: Tumor-plate8-37 [EpCam and CD45]


title: Tumor-plate8-29 [EpCam and CD45]


title: Tumor-plate8-27 [EpCam and CD45]


title: Tumor-plate8-26 [EpCam and CD45]


title: Tumor-plate8-21 [EpCam and CD45]


title: Tumor-plate8-23 [EpCam and CD45]


title: Tumor-plate8-25 [EpCam and CD45]


title: Tumor-plate8-18 [EpCam and CD45]


title: Tumor-plate8-22 [EpCam and CD45]


title: Tumor-plate8-20 [EpCam and CD45]


title: Tumor-plate8-2 [EpCam and CD45]


title: Tumor-plate8-19 [EpCam and CD45]


title: Tumor-plate8-16 [EpCam and CD45]


title: Tumor-plate8-24 [EpCam and CD45]


title: Tumor-plate8-14 [EpCam and CD45]


title: Tumor-plate8-17 [EpCam and CD45]


title: Tumor-plate8-12 [EpCam and CD45]


title: Tumor-plate8-11 [EpCam and CD45]


title: Tumor-plate8-13 [EpCam and CD45]


title: Tumor-plate8-10 [EpCam and CD45 ]


title: Tumor-plate7-96 [EpCam and HIF1a]

description: Freshly isolated cells from a breast tumor were fixed, permeabilized and stained against EpCam and HIF1a ,  then sorted into single wells of a 96 well plate for MI-ATAC

value: EpCam and HIF1a


title: Tumor-plate7-95 [EpCam and HIF1a]


title: Tumor-plate7-94 [EpCam and HIF1a]


title: Tumor-plate7-92 [EpCam and HIF1a]


title: Tumor-plate7-91 [EpCam and HIF1a]


title: Tumor-plate7-90 [EpCam and HIF1a]


title: Tumor-plate7-9 [EpCam and HIF1a]


title: Tumor-plate7-93 [EpCam and HIF1a]


title: Tumor-plate7-89 [EpCam and HIF1a]


title: Tumor-plate7-88 [EpCam and HIF1a]


title: Tumor-plate7-87 [EpCam and HIF1a]


title: Tumor-plate7-85 [EpCam and HIF1a]


title: Tumor-plate7-86 [EpCam and HIF1a]


title: Tumor-plate7-82 [EpCam and HIF1a]


title: Tumor-plate7-77 [EpCam and HIF1a]


title: Tumor-plate7-80 [EpCam and HIF1a]


title: Tumor-plate8-15 [EpCam and CD45]


title: Tumor-plate7-8 [EpCam and HIF1a]


title: Tumor-plate7-84 [EpCam and HIF1a]


title: Tumor-plate7-81 [EpCam and HIF1a]


title: Tumor-plate7-76 [EpCam and HIF1a]


title: Tumor-plate7-79 [EpCam and HIF1a]


title: Tumor-plate7-78 [EpCam and HIF1a]


title: Tumor-plate7-73 [EpCam and HIF1a]


title: Tumor-plate7-83 [EpCam and HIF1a]


title: Tumor-plate7-74 [EpCam and HIF1a]


title: Tumor-plate7-75 [EpCam and HIF1a]


title: Tumor-plate7-72 [EpCam and HIF1a]


title: Tumor-plate8-1 [EpCam and CD45 ]


title: Tumor-plate7-7 [EpCam and HIF1a]


title: Tumor-plate7-68 [EpCam and HIF1a]


title: Tumor-plate7-69 [EpCam and HIF1a]


title: Tumor-plate7-67 [EpCam and HIF1a]


title: Tumor-plate7-70 [EpCam and HIF1a]


title: Tumor-plate7-65 [EpCam and HIF1a]


title: Tumor-plate7-66 [EpCam and HIF1a]


title: Tumor-plate7-64 [EpCam and HIF1a]


title: Tumor-plate7-63 [EpCam and HIF1a]


title: Tumor-plate7-62 [EpCam and HIF1a]


title: Tumor-plate7-61 [EpCam and HIF1a]


title: Tumor-plate7-6 [EpCam and HIF1a]


title: Tumor-plate7-60 [EpCam and HIF1a]


title: Tumor-plate7-59 [EpCam and HIF1a]


title: Tumor-plate7-57 [EpCam and HIF1a]


title: Tumor-plate7-56 [EpCam and HIF1a]


title: Tumor-plate7-55 [EpCam and HIF1a]


title: Tumor-plate7-54 [EpCam and HIF1a]


title: Tumor-plate7-52 [EpCam and HIF1a]


title: Tumor-plate7-71 [EpCam and HIF1a]


title: Tumor-plate7-51 [EpCam and HIF1a]


title: Tumor-plate7-53 [EpCam and HIF1a]


title: Tumor-plate7-5 [EpCam and HIF1a]


title: Tumor-plate7-45 [EpCam and HIF1a]


title: Tumor-plate7-47 [EpCam and HIF1a]


title: Tumor-plate7-49 [EpCam and HIF1a]


title: Tumor-plate7-48 [EpCam and HIF1a]


title: Tumor-plate7-44 [EpCam and HIF1a]


title: Tumor-plate7-42 [EpCam and HIF1a]


title: Tumor-plate7-40 [EpCam and HIF1a]


title: Tumor-plate7-43 [EpCam and HIF1a]


title: Tumor-plate7-39 [EpCam and HIF1a]


title: Tumor-plate7-36 [EpCam and HIF1a]


title: Tumor-plate7-35 [EpCam and HIF1a]


title: Tumor-plate7-58 [EpCam and HIF1a]


title: Tumor-plate7-37 [EpCam and HIF1a]


title: Tumor-plate7-34 [EpCam and HIF1a]


title: Tumor-plate7-33 [EpCam and HIF1a]


title: Tumor-plate7-32 [EpCam and HIF1a]


title: Tumor-plate7-29 [EpCam and HIF1a]


title: Tumor-plate7-30 [EpCam and HIF1a]


title: Tumor-plate7-31 [EpCam and HIF1a]


title: Tumor-plate7-27 [EpCam and HIF1a]


title: Tumor-plate7-50 [EpCam and HIF1a]


title: Tumor-plate7-26 [EpCam and HIF1a]


title: Tumor-plate7-46 [EpCam and HIF1a]


title: Tumor-plate7-24 [EpCam and HIF1a]


title: Tumor-plate7-38 [EpCam and HIF1a]


title: Tumor-plate7-4 [EpCam and HIF1a]


title: Tumor-plate7-28 [EpCam and HIF1a]


title: Tumor-plate7-3 [EpCam and HIF1a]


title: Tumor-plate7-23 [EpCam and HIF1a]


title: Tumor-plate7-21 [EpCam and HIF1a]


title: Tumor-plate7-41 [EpCam and HIF1a]


title: Tumor-plate7-20 [EpCam and HIF1a]


title: Tumor-plate7-19 [EpCam and HIF1a]


title: Tumor-plate7-17 [EpCam and HIF1a]


title: Tumor-plate7-22 [EpCam and HIF1a]


title: Tumor-plate7-2 [EpCam and HIF1a]


title: Tumor-plate7-15 [EpCam and HIF1a]


title: Tumor-plate7-14 [EpCam and HIF1a]


title: Tumor-plate7-13 [EpCam and HIF1a]


title: Tumor-plate7-10 [EpCam and HIF1a]


title: Tumor-plate7-18 [EpCam and HIF1a]


title: Tumor-plate7-11 [EpCam and HIF1a]


title: Tumor-plate7-16 [EpCam and HIF1a]


title: Tumor-plate7-1 [EpCam and HIF1a]


title: Tumor-plate7-25 [EpCam and HIF1a]


title: Tumor-plate6-96 [EpCam and HIF1a]


title: Tumor-plate6-92 [EpCam and HIF1a]


title: Tumor-plate6-93 [EpCam and HIF1a]


title: Tumor-plate6-94 [EpCam and HIF1a]


title: Tumor-plate6-9 [EpCam and HIF1a]


title: Tumor-plate6-95 [EpCam and HIF1a]


title: Tumor-plate6-90 [EpCam and HIF1a]


title: Tumor-plate6-91 [EpCam and HIF1a]


title: Tumor-plate6-88 [EpCam and HIF1a]


title: Tumor-plate6-87 [EpCam and HIF1a]


title: Tumor-plate6-86 [EpCam and HIF1a]


title: Tumor-plate6-81 [EpCam and HIF1a]


title: Tumor-plate6-89 [EpCam and HIF1a]


title: Tumor-plate6-85 [EpCam and HIF1a]


title: Tumor-plate6-80 [EpCam and HIF1a]


title: Tumor-plate7-12 [EpCam and HIF1a]


title: Tumor-plate6-83 [EpCam and HIF1a]


title: Tumor-plate6-8 [EpCam and HIF1a]


title: Tumor-plate6-75 [EpCam and HIF1a]


title: Tumor-plate6-76 [EpCam and HIF1a]


title: Tumor-plate6-82 [EpCam and HIF1a]


title: Tumor-plate6-79 [EpCam and HIF1a]


title: Tumor-plate6-77 [EpCam and HIF1a]


title: Tumor-plate6-74 [EpCam and HIF1a]


title: Tumor-plate6-78 [EpCam and HIF1a]


title: Tumor-plate6-70 [EpCam and HIF1a]


title: Tumor-plate6-71 [EpCam and HIF1a]


title: Tumor-plate6-73 [EpCam and HIF1a]


title: Tumor-plate6-69 [EpCam and HIF1a]


title: Tumor-plate6-84 [EpCam and HIF1a]


title: Tumor-plate6-68 [EpCam and HIF1a]


title: Tumor-plate6-62 [EpCam and HIF1a]


title: Tumor-plate6-65 [EpCam and HIF1a]


title: Tumor-plate6-60 [EpCam and HIF1a]


title: Tumor-plate6-61 [EpCam and HIF1a]


title: Tumor-plate6-63 [EpCam and HIF1a]


title: Tumor-plate6-59 [EpCam and HIF1a]


title: Tumor-plate6-6 [EpCam and HIF1a]


title: Tumor-plate6-57 [EpCam and HIF1a]


title: Tumor-plate6-55 [EpCam and HIF1a]


title: Tumor-plate6-72 [EpCam and HIF1a]


title: Tumor-plate6-56 [EpCam and HIF1a]


title: Tumor-plate6-54 [EpCam and HIF1a]


title: Tumor-plate6-53 [EpCam and HIF1a]


title: Tumor-plate6-52 [EpCam and HIF1a]


title: Tumor-plate6-58 [EpCam and HIF1a]


title: Tumor-plate6-5 [EpCam and HIF1a]


title: Tumor-plate6-48 [EpCam and HIF1a]


title: Tumor-plate6-64 [EpCam and HIF1a]


title: Tumor-plate6-50 [EpCam and HIF1a]


title: Tumor-plate6-47 [EpCam and HIF1a]


title: Tumor-plate6-7 [EpCam and HIF1a]


title: Tumor-plate6-49 [EpCam and HIF1a]


title: Tumor-plate6-51 [EpCam and HIF1a]


title: Tumor-plate6-66 [EpCam and HIF1a]


title: Tumor-plate6-46 [EpCam and HIF1a]


title: Tumor-plate6-67 [EpCam and HIF1a]


title: Tumor-plate6-43 [EpCam and HIF1a]


title: Tumor-plate6-41 [EpCam and HIF1a]


title: Tumor-plate6-42 [EpCam and HIF1a]


title: Tumor-plate6-39 [EpCam and HIF1a]


title: Tumor-plate6-36 [EpCam and HIF1a]


title: Tumor-plate6-37 [EpCam and HIF1a]


title: Tumor-plate6-38 [EpCam and HIF1a]


title: Tumor-plate6-40 [EpCam and HIF1a]


title: Tumor-plate6-34 [EpCam and HIF1a]


title: Tumor-plate6-35 [EpCam and HIF1a]


title: Tumor-plate6-33 [EpCam and HIF1a]


title: Tumor-plate6-45 [EpCam and HIF1a]


title: Tumor-plate6-30 [EpCam and HIF1a]


title: Tumor-plate6-32 [EpCam and HIF1a]


title: Tumor-plate6-31 [EpCam and HIF1a]


title: Tumor-plate6-25 [EpCam and HIF1a]


title: Tumor-plate6-3 [EpCam and HIF1a]


title: Tumor-plate6-27 [EpCam and HIF1a]


title: Tumor-plate6-24 [EpCam and HIF1a]


title: Tumor-plate6-29 [EpCam and HIF1a]


title: Tumor-plate6-26 [EpCam and HIF1a]


title: Tumor-plate6-44 [EpCam and HIF1a]


title: Tumor-plate6-23 [EpCam and HIF1a]


title: Tumor-plate6-20 [EpCam and HIF1a]


title: Tumor-plate6-19 [EpCam and HIF1a]


title: Tumor-plate6-4 [EpCam and HIF1a]


title: Tumor-plate6-17 [EpCam and HIF1a]


title: Tumor-plate6-18 [EpCam and HIF1a]


title: Tumor-plate6-12 [EpCam and HIF1a]


title: Tumor-plate6-28 [EpCam and HIF1a]


title: Tumor-plate6-13 [EpCam and HIF1a]


title: Tumor-plate6-16 [EpCam and HIF1a]


title: Tumor-plate6-11 [EpCam and HIF1a]


title: Tumor-plate6-15 [EpCam and HIF1a]


title: Tumor-plate6-14 [EpCam and HIF1a]


title: Tumor-plate6-10 [EpCam and HIF1a]


title: Tumor-plate6-22 [EpCam and HIF1a]


title: Tumor-plate5-88 [EpCam and HIF1a]


title: Tumor-plate5-9 [EpCam and HIF1a]


title: Tumor-plate5-93 [EpCam and HIF1a]


title: Tumor-plate6-2 [EpCam and HIF1a]


title: Tumor-plate5-86 [EpCam and HIF1a]


title: Tumor-plate5-85 [EpCam and HIF1a]


title: Tumor-plate5-91 [EpCam and HIF1a]


title: Tumor-plate5-92 [EpCam and HIF1a]


title: Tumor-plate6-21 [EpCam and HIF1a]


title: Tumor-plate5-89 [EpCam and HIF1a]


title: Tumor-plate5-96 [EpCam and HIF1a]


title: Tumor-plate5-90 [EpCam and HIF1a]


title: Tumor-plate5-84 [EpCam and HIF1a]


title: Tumor-plate5-87 [EpCam and HIF1a]


title: Tumor-plate5-83 [EpCam and HIF1a]


title: Tumor-plate5-81 [EpCam and HIF1a]


title: Tumor-plate5-80 [EpCam and HIF1a]


title: Tumor-plate5-82 [EpCam and HIF1a]


title: Tumor-plate6-1 [EpCam and HIF1a]


title: Tumor-plate5-95 [EpCam and HIF1a]


title: Tumor-plate5-79 [EpCam and HIF1a]


title: Tumor-plate5-76 [EpCam and HIF1a]


title: Tumor-plate5-77 [EpCam and HIF1a]


title: Tumor-plate5-75 [EpCam and HIF1a]


title: Tumor-plate5-73 [EpCam and HIF1a]


title: Tumor-plate5-94 [EpCam and HIF1a]


title: Tumor-plate5-71 [EpCam and HIF1a]


title: Tumor-plate5-69 [EpCam and HIF1a]


title: Tumor-plate5-65 [EpCam and HIF1a]


title: Tumor-plate5-67 [EpCam and HIF1a]


title: Tumor-plate5-68 [EpCam and HIF1a]


title: Tumor-plate5-7 [EpCam and HIF1a]


title: Tumor-plate5-62 [EpCam and HIF1a]


title: Tumor-plate5-61 [EpCam and HIF1a]


title: Tumor-plate5-63 [EpCam and HIF1a]


title: Tumor-plate5-60 [EpCam and HIF1a]


title: Tumor-plate5-64 [EpCam and HIF1a]


title: Tumor-plate5-8 [EpCam and HIF1a]


title: Tumor-plate5-56 [EpCam and HIF1a]


title: Tumor-plate5-59 [EpCam and HIF1a]


title: Tumor-plate5-58 [EpCam and HIF1a]


title: Tumor-plate5-57 [EpCam and HIF1a]


title: Tumor-plate5-74 [EpCam and HIF1a]


title: Tumor-plate5-54 [EpCam and HIF1a]


title: Tumor-plate5-55 [EpCam and HIF1a]


title: Tumor-plate5-52 [EpCam and HIF1a]


title: Tumor-plate5-6 [EpCam and HIF1a]


title: Tumor-plate5-78 [EpCam and HIF1a]


title: Tumor-plate5-5 [EpCam and HIF1a]


title: Tumor-plate5-49 [EpCam and HIF1a]


title: Tumor-plate5-51 [EpCam and HIF1a]


title: Tumor-plate5-53 [EpCam and HIF1a]


title: Tumor-plate5-70 [EpCam and HIF1a]


title: Tumor-plate5-48 [EpCam and HIF1a]


title: Tumor-plate5-66 [EpCam and HIF1a]


title: Tumor-plate5-47 [EpCam and HIF1a]


title: Tumor-plate5-43 [EpCam and HIF1a]


title: Tumor-plate5-44 [EpCam and HIF1a]


title: Tumor-plate5-45 [EpCam and HIF1a]


title: Tumor-plate5-41 [EpCam and HIF1a]


title: Tumor-plate5-40 [EpCam and HIF1a]


title: Tumor-plate5-42 [EpCam and HIF1a]


title: Tumor-plate5-4 [EpCam and HIF1a]


title: Tumor-plate5-38 [EpCam and HIF1a]


title: Tumor-plate5-50 [EpCam and HIF1a]


title: Tumor-plate5-36 [EpCam and HIF1a]


title: Tumor-plate5-72 [EpCam and HIF1a]


title: Tumor-plate5-37 [EpCam and HIF1a]


title: Tumor-plate5-39 [EpCam and HIF1a]


title: Tumor-plate5-34 [EpCam and HIF1a]


title: Tumor-plate5-35 [EpCam and HIF1a]


title: Tumor-plate5-33 [EpCam and HIF1a]


title: Tumor-plate5-32 [EpCam and HIF1a]


title: Tumor-plate5-3 [EpCam and HIF1a]


title: Tumor-plate5-30 [EpCam and HIF1a]


title: Tumor-plate5-27 [EpCam and HIF1a]


title: Tumor-plate5-26 [EpCam and HIF1a]


title: Tumor-plate5-31 [EpCam and HIF1a]


title: Tumor-plate5-29 [EpCam and HIF1a]


title: Tumor-plate5-28 [EpCam and HIF1a]


title: Tumor-plate5-46 [EpCam and HIF1a]


title: Tumor-plate5-24 [EpCam and HIF1a]


title: Tumor-plate5-23 [EpCam and HIF1a]


title: Tumor-plate5-22 [EpCam and HIF1a]


title: Tumor-plate5-21 [EpCam and HIF1a]


title: Tumor-plate5-20 [EpCam and HIF1a]


title: Tumor-plate5-19 [EpCam and HIF1a]


title: Tumor-plate5-2 [EpCam and HIF1a]


title: Tumor-plate5-15 [EpCam and HIF1a]


title: Tumor-plate5-16 [EpCam and HIF1a]


title: Tumor-plate5-14 [EpCam and HIF1a]


title: Tumor-plate5-18 [EpCam and HIF1a]


title: Tumor-plate5-17 [EpCam and HIF1a]


title: Tumor-plate5-11 [EpCam and HIF1a]


title: Tumor-plate5-12 [EpCam and HIF1a]


title: Tumor-plate5-13 [EpCam and HIF1a]


title: Tumor-plate4-96 [EpCam and HIF1a]


title: Tumor-plate5-25 [EpCam and HIF1a]


title: Tumor-plate4-92 [EpCam and HIF1a]


title: Tumor-plate5-10 [EpCam and HIF1a]


title: Tumor-plate4-91 [EpCam and HIF1a]


title: Tumor-plate5-1 [EpCam and HIF1a]


title: Tumor-plate4-94 [EpCam and HIF1a]


title: Tumor-plate4-90 [EpCam and HIF1a]


title: Tumor-plate4-9 [EpCam and HIF1a]


title: Tumor-plate4-93 [EpCam and HIF1a]


title: Tumor-plate4-95 [EpCam and HIF1a]


title: Tumor-plate4-89 [EpCam and HIF1a]


title: Tumor-plate4-85 [EpCam and HIF1a]


title: Tumor-plate4-83 [EpCam and HIF1a]


title: Tumor-plate4-84 [EpCam and HIF1a]


title: Tumor-plate4-82 [EpCam and HIF1a]


title: Tumor-plate4-80 [EpCam and HIF1a]


title: Tumor-plate4-87 [EpCam and HIF1a]


title: Tumor-plate4-88 [EpCam and HIF1a]


title: Tumor-plate4-8 [EpCam and HIF1a]


title: Tumor-plate4-81 [EpCam and HIF1a]


title: Tumor-plate4-79 [EpCam and HIF1a]


title: Tumor-plate4-75 [EpCam and HIF1a]


title: Tumor-plate4-78 [EpCam and HIF1a]


title: Tumor-plate4-76 [EpCam and HIF1a]


title: Tumor-plate4-73 [EpCam and HIF1a]


title: Tumor-plate4-71 [EpCam and HIF1a]


title: Tumor-plate4-74 [EpCam and HIF1a]


title: Tumor-plate4-70 [EpCam and HIF1a]


title: Tumor-plate4-72 [EpCam and HIF1a]


title: Tumor-plate4-7 [EpCam and HIF1a]


title: Tumor-plate4-69 [EpCam and HIF1a]


title: Tumor-plate4-66 [EpCam and HIF1a]


title: Tumor-plate4-65 [EpCam and HIF1a]


title: Tumor-plate4-67 [EpCam and HIF1a]


title: Tumor-plate4-86 [EpCam and HIF1a]


title: Tumor-plate4-63 [EpCam and HIF1a]


title: Tumor-plate4-64 [EpCam and HIF1a]


title: Tumor-plate4-62 [EpCam and HIF1a]


title: Tumor-plate4-60 [EpCam and HIF1a]


title: Tumor-plate4-6 [EpCam and HIF1a]


title: Tumor-plate4-61 [EpCam and HIF1a]


title: Tumor-plate4-57 [EpCam and HIF1a]


title: Tumor-plate4-58 [EpCam and HIF1a]


title: Tumor-plate4-54 [EpCam and HIF1a]


title: Tumor-plate4-53 [EpCam and HIF1a]


title: Tumor-plate4-56 [EpCam and HIF1a]


title: Tumor-plate4-51 [EpCam and HIF1a]


title: Tumor-plate4-50 [EpCam and HIF1a]


title: Tumor-plate4-49 [EpCam and HIF1a]


title: Tumor-plate4-46 [EpCam and HIF1a]


title: Tumor-plate4-68 [EpCam and HIF1a]


title: Tumor-plate4-47 [EpCam and HIF1a]


title: Tumor-plate4-45 [EpCam and HIF1a]


title: Tumor-plate4-5 [EpCam and HIF1a]


title: Tumor-plate4-48 [EpCam and HIF1a]


title: Tumor-plate4-44 [EpCam and HIF1a]


title: Tumor-plate4-43 [EpCam and HIF1a]


title: Tumor-plate4-52 [EpCam and HIF1a]


title: Tumor-plate4-40 [EpCam and HIF1a]


title: Tumor-plate4-41 [EpCam and HIF1a]


title: Tumor-plate4-42 [EpCam and HIF1a]


title: Tumor-plate4-39 [EpCam and HIF1a]


title: Tumor-plate4-4 [EpCam and HIF1a]


title: Tumor-plate4-59 [EpCam and HIF1a]


title: Tumor-plate4-77 [EpCam and HIF1a]


title: Tumor-plate4-35 [EpCam and HIF1a]


title: Tumor-plate4-33 [EpCam and HIF1a]


title: Tumor-plate4-37 [EpCam and HIF1a]


title: Tumor-plate4-34 [EpCam and HIF1a]


title: Tumor-plate4-36 [EpCam and HIF1a]


title: Tumor-plate4-31 [EpCam and HIF1a]


title: Tumor-plate4-32 [EpCam and HIF1a]


title: Tumor-plate4-3 [EpCam and HIF1a]


title: Tumor-plate4-29 [EpCam and HIF1a]


title: Tumor-plate4-30 [EpCam and HIF1a]


title: Tumor-plate4-28 [EpCam and HIF1a]


title: Tumor-plate4-27 [EpCam and HIF1a]


title: Tumor-plate4-26 [EpCam and HIF1a]


title: Tumor-plate4-23 [EpCam and HIF1a]


title: Tumor-plate4-21 [EpCam and HIF1a]


title: Tumor-plate4-25 [EpCam and HIF1a]


title: Tumor-plate4-24 [EpCam and HIF1a]


title: Tumor-plate4-20 [EpCam and HIF1a]


title: Tumor-plate4-2 [EpCam and HIF1a]


title: Tumor-plate4-18 [EpCam and HIF1a]


title: Tumor-plate4-16 [EpCam and HIF1a]


title: Tumor-plate4-38 [EpCam and HIF1a]


title: Tumor-plate4-14 [EpCam and HIF1a]


title: Tumor-plate4-15 [EpCam and HIF1a]


title: Tumor-plate4-19 [EpCam and HIF1a]


title: Tumor-plate4-17 [EpCam and HIF1a]


title: Tumor-plate4-12 [EpCam and HIF1a]


title: Tumor-plate4-13 [EpCam and HIF1a]


title: Tumor-plate4-55 [EpCam and HIF1a]


title: Tumor-plate3-95 [EpCam and HIF1a]


title: Tumor-plate3-96 [EpCam and HIF1a]


title: Tumor-plate4-11 [EpCam and HIF1a]


title: Tumor-plate3-94 [EpCam and HIF1a]


title: Tumor-plate3-93 [EpCam and HIF1a]


title: Tumor-plate3-90 [EpCam and HIF1a]


title: Tumor-plate4-22 [EpCam and HIF1a]


title: Tumor-plate3-89 [EpCam and HIF1a]


title: Tumor-plate4-10 [EpCam and HIF1a]


title: Tumor-plate3-9 [EpCam and HIF1a]


title: Tumor-plate3-88 [EpCam and HIF1a]


title: Tumor-plate3-85 [EpCam and HIF1a]


title: Tumor-plate3-87 [EpCam and HIF1a]


title: Tumor-plate3-86 [EpCam and HIF1a]


title: Tumor-plate3-82 [EpCam and HIF1a]


title: Tumor-plate3-84 [EpCam and HIF1a]


title: Tumor-plate3-8 [EpCam and HIF1a]


title: Tumor-plate3-78 [EpCam and HIF1a]


title: Tumor-plate3-79 [EpCam and HIF1a]


title: Tumor-plate3-76 [EpCam and HIF1a]


title: Tumor-plate4-1 [EpCam and HIF1a]


title: Tumor-plate3-81 [EpCam and HIF1a]


title: Tumor-plate3-75 [EpCam and HIF1a]


title: Tumor-plate3-73 [EpCam and HIF1a]


title: Tumor-plate3-92 [EpCam and HIF1a]


title: Tumor-plate3-80 [EpCam and HIF1a]


title: Tumor-plate3-71 [EpCam and HIF1a]


title: Tumor-plate3-70 [EpCam and HIF1a]


title: Tumor-plate3-91 [EpCam and HIF1a]


title: Tumor-plate3-72 [EpCam and HIF1a]


title: Tumor-plate3-7 [EpCam and HIF1a]


title: Tumor-plate3-74 [EpCam and HIF1a]


title: Tumor-plate3-77 [EpCam and HIF1a]


title: Tumor-plate3-69 [EpCam and HIF1a]


title: Tumor-plate3-65 [EpCam and HIF1a]


title: Tumor-plate3-67 [EpCam and HIF1a]


title: Tumor-plate3-66 [EpCam and HIF1a]


title: Tumor-plate3-6 [EpCam and HIF1a]


title: Tumor-plate3-64 [EpCam and HIF1a]


title: Tumor-plate3-83 [EpCam and HIF1a]


title: Tumor-plate3-59 [EpCam and HIF1a]


title: Tumor-plate3-57 [EpCam and HIF1a]


title: Tumor-plate3-63 [EpCam and HIF1a]


title: Tumor-plate3-58 [EpCam and HIF1a]


title: Tumor-plate3-52 [EpCam and HIF1a]


title: Tumor-plate3-53 [EpCam and HIF1a]


title: Tumor-plate3-61 [EpCam and HIF1a]


title: Tumor-plate3-50 [EpCam and HIF1a]


title: Tumor-plate3-54 [EpCam and HIF1a]


title: Tumor-plate3-5 [EpCam and HIF1a]


title: Tumor-plate3-62 [EpCam and HIF1a]


title: Tumor-plate3-56 [EpCam and HIF1a]


title: Tumor-plate3-55 [EpCam and HIF1a]


title: Tumor-plate3-48 [EpCam and HIF1a]


title: Tumor-plate3-47 [EpCam and HIF1a]


title: Tumor-plate3-51 [EpCam and HIF1a]


title: Tumor-plate3-49 [EpCam and HIF1a]


title: Tumor-plate3-68 [EpCam and HIF1a]


title: Tumor-plate3-45 [EpCam and HIF1a]


title: Tumor-plate3-46 [EpCam and HIF1a]


title: Tumor-plate3-44 [EpCam and HIF1a]


title: Tumor-plate3-43 [EpCam and HIF1a]


title: Tumor-plate3-39 [EpCam and HIF1a]


title: Tumor-plate3-60 [EpCam and HIF1a]


title: Tumor-plate3-42 [EpCam and HIF1a]


title: Tumor-plate3-37 [EpCam and HIF1a]


title: Tumor-plate3-35 [EpCam and HIF1a]


title: Tumor-plate3-40 [EpCam and HIF1a]


title: Tumor-plate3-32 [EpCam and HIF1a]


title: Tumor-plate3-29 [EpCam and HIF1a]


title: Tumor-plate3-3 [EpCam and HIF1a]


title: Tumor-plate3-33 [EpCam and HIF1a]


title: Tumor-plate3-34 [EpCam and HIF1a]


title: Tumor-plate3-25 [EpCam and HIF1a]


title: Tumor-plate3-27 [EpCam and HIF1a]


title: Tumor-plate3-28 [EpCam and HIF1a]


title: Tumor-plate3-31 [EpCam and HIF1a]


title: Tumor-plate3-36 [EpCam and HIF1a]


title: Tumor-plate3-23 [EpCam and HIF1a]


title: Tumor-plate3-26 [EpCam and HIF1a]


title: Tumor-plate3-21 [EpCam and HIF1a]


title: Tumor-plate3-4 [EpCam and HIF1a]


title: Tumor-plate3-24 [EpCam and HIF1a]


title: Tumor-plate3-41 [EpCam and HIF1a]


title: Tumor-plate3-30 [EpCam and HIF1a]


title: Tumor-plate3-19 [EpCam and HIF1a]


title: Tumor-plate3-38 [EpCam and HIF1a]


title: Tumor-plate3-20 [EpCam and HIF1a]


title: Tumor-plate3-18 [EpCam and HIF1a]


title: Tumor-plate3-15 [EpCam and HIF1a]


title: Tumor-plate3-14 [EpCam and HIF1a]


title: Tumor-plate3-1 [EpCam and HIF1a]


title: Tumor-plate2-96 [EpCam and HIF1a]


title: Tumor-plate3-10 [EpCam and HIF1a]


title: Tumor-plate3-13 [EpCam and HIF1a]


title: Tumor-plate3-11 [EpCam and HIF1a]


title: Tumor-plate3-12 [EpCam and HIF1a]


title: Tumor-plate3-22 [EpCam and HIF1a]


title: Tumor-plate2-91 [EpCam and HIF1a]


title: Tumor-plate2-93 [EpCam and HIF1a]


title: Tumor-plate2-92 [EpCam and HIF1a]


title: Tumor-plate2-94 [EpCam and HIF1a]


title: Tumor-plate2-90 [EpCam and HIF1a]


title: Tumor-plate2-89 [EpCam and HIF1a]


title: Tumor-plate2-95 [EpCam and HIF1a]


title: Tumor-plate2-9 [EpCam and HIF1a]


title: Tumor-plate2-85 [EpCam and HIF1a]


title: Tumor-plate2-86 [EpCam and HIF1a]


title: Tumor-plate2-87 [EpCam and HIF1a]


title: Tumor-plate3-2 [EpCam and HIF1a]


title: Tumor-plate2-83 [EpCam and HIF1a]


title: Tumor-plate2-82 [EpCam and HIF1a]


title: Tumor-plate2-88 [EpCam and HIF1a]


title: Tumor-plate2-8 [EpCam and HIF1a]


title: Tumor-plate3-17 [EpCam and HIF1a]


title: Tumor-plate2-84 [EpCam and HIF1a]


title: Tumor-plate3-16 [EpCam and HIF1a]


title: Tumor-plate2-81 [EpCam and HIF1a]


title: Tumor-plate2-80 [EpCam and HIF1a]


title: Tumor-plate2-79 [EpCam and HIF1a]


title: Tumor-plate2-77 [EpCam and HIF1a]


title: Tumor-plate2-74 [EpCam and HIF1a]


title: Tumor-plate2-76 [EpCam and HIF1a]


title: Tumor-plate2-73 [EpCam and HIF1a]


title: Tumor-plate2-75 [EpCam and HIF1a]


title: Tumor-plate2-66 [EpCam and HIF1a]


title: Tumor-plate2-70 [EpCam and HIF1a]


title: Tumor-plate2-69 [EpCam and HIF1a]


title: Tumor-plate2-65 [EpCam and HIF1a]


title: Tumor-plate2-68 [EpCam and HIF1a]


title: Tumor-plate2-71 [EpCam and HIF1a]


title: Tumor-plate2-62 [EpCam and HIF1a]


title: Tumor-plate2-60 [EpCam and HIF1a]


title: Tumor-plate2-64 [EpCam and HIF1a]


title: Tumor-plate2-6 [EpCam and HIF1a]


title: Tumor-plate2-59 [EpCam and HIF1a]


title: Tumor-plate2-57 [EpCam and HIF1a]


title: Tumor-plate2-63 [EpCam and HIF1a]


title: Tumor-plate2-58 [EpCam and HIF1a]


title: Tumor-plate2-78 [EpCam and HIF1a]


title: Tumor-plate2-56 [EpCam and HIF1a]


title: Tumor-plate2-72 [EpCam and HIF1a]


title: Tumor-plate2-55 [EpCam and HIF1a]


title: Tumor-plate2-67 [EpCam and HIF1a]


title: Tumor-plate2-53 [EpCam and HIF1a]


title: Tumor-plate2-51 [EpCam and HIF1a]


title: Tumor-plate2-54 [EpCam and HIF1a]


title: Tumor-plate2-7 [EpCam and HIF1a]


title: Tumor-plate2-61 [EpCam and HIF1a]


title: Tumor-plate2-46 [EpCam and HIF1a]


title: Tumor-plate2-50 [EpCam and HIF1a]


title: Tumor-plate2-48 [EpCam and HIF1a]


title: Tumor-plate2-49 [EpCam and HIF1a]


title: Tumor-plate2-44 [EpCam and HIF1a]


title: Tumor-plate2-41 [EpCam and HIF1a]


title: Tumor-plate2-42 [EpCam and HIF1a]


title: Tumor-plate2-47 [EpCam and HIF1a]


title: Tumor-plate2-4 [EpCam and HIF1a]


title: Tumor-plate2-52 [EpCam and HIF1a]


title: Tumor-plate2-43 [EpCam and HIF1a]


title: Tumor-plate2-40 [EpCam and HIF1a]


title: Tumor-plate2-38 [EpCam and HIF1a]


title: Tumor-plate2-5 [EpCam and HIF1a]


title: Tumor-plate2-36 [EpCam and HIF1a]


title: Tumor-plate2-34 [EpCam and HIF1a]


title: Tumor-plate2-33 [EpCam and HIF1a]


title: Tumor-plate2-32 [EpCam and HIF1a]


title: Tumor-plate2-31 [EpCam and HIF1a]


title: Tumor-plate2-30 [EpCam and HIF1a]


title: Tumor-plate2-45 [EpCam and HIF1a]


title: Tumor-plate2-3 [EpCam and HIF1a]


title: Tumor-plate2-27 [EpCam and HIF1a]


title: Tumor-plate2-28 [EpCam and HIF1a]


title: Tumor-plate2-39 [EpCam and HIF1a]


title: Tumor-plate2-29 [EpCam and HIF1a]


title: Tumor-plate2-26 [EpCam and HIF1a]


title: Tumor-plate2-23 [EpCam and HIF1a]


title: Tumor-plate2-24 [EpCam and HIF1a]


title: Tumor-plate2-25 [EpCam and HIF1a]


title: Tumor-plate2-37 [EpCam and HIF1a]


title: Tumor-plate2-14 [EpCam and HIF1a]


title: Tumor-plate2-17 [EpCam and HIF1a]


title: Tumor-plate2-19 [EpCam and HIF1a]


title: Tumor-plate2-20 [EpCam and HIF1a]


title: Tumor-plate2-15 [EpCam and HIF1a]


title: Tumor-plate2-22 [EpCam and HIF1a]


title: Tumor-plate2-2 [EpCam and HIF1a]


title: Tumor-plate2-35 [EpCam and HIF1a]


title: Tumor-plate2-12 [EpCam and HIF1a]


title: Tumor-plate2-11 [EpCam and HIF1a]


title: Tumor-plate2-16 [EpCam and HIF1a]


title: Tumor-plate2-21 [EpCam and HIF1a]


title: Tumor-plate2-10 [EpCam and HIF1a]


title: Tumor-plate2-1 [EpCam and HIF1a]


title: Tumor-plate10-95 [EpCam and HIF1a]


title: Tumor-plate10-96 [EpCam and HIF1a]


title: Tumor-plate10-94 [EpCam and HIF1a]


title: Tumor-plate10-93 [EpCam and HIF1a]


title: Tumor-plate10-92 [EpCam and HIF1a]


title: Tumor-plate10-91 [EpCam and HIF1a]


title: Tumor-plate10-90 [EpCam and HIF1a]


title: Tumor-plate2-18 [EpCam and HIF1a]


title: Tumor-plate10-89 [EpCam and HIF1a]


title: Tumor-plate10-87 [EpCam and HIF1a]


title: Tumor-plate10-84 [EpCam and HIF1a]


title: Tumor-plate10-9 [EpCam and HIF1a]


title: Tumor-plate10-85 [EpCam and HIF1a]


title: Tumor-plate2-13 [EpCam and HIF1a]


title: Tumor-plate10-88 [EpCam and HIF1a]


title: Tumor-plate10-86 [EpCam and HIF1a]


title: Tumor-plate10-83 [EpCam and HIF1a]


title: Tumor-plate10-78 [EpCam and HIF1a]


title: Tumor-plate10-76 [EpCam and HIF1a]


title: Tumor-plate10-77 [EpCam and HIF1a]


title: Tumor-plate10-75 [EpCam and HIF1a]


title: Tumor-plate10-8 [EpCam and HIF1a]


title: Tumor-plate10-79 [EpCam and HIF1a]


title: Tumor-plate10-72 [EpCam and HIF1a]


title: Tumor-plate10-82 [EpCam and HIF1a]


title: Tumor-plate10-73 [EpCam and HIF1a]


title: Tumor-plate10-80 [EpCam and HIF1a]


title: Tumor-plate10-74 [EpCam and HIF1a]


title: Tumor-plate10-71 [EpCam and HIF1a]


title: Tumor-plate10-7 [EpCam and HIF1a]


title: Tumor-plate10-70 [EpCam and HIF1a]


title: Tumor-plate10-69 [EpCam and HIF1a]


title: Tumor-plate10-68 [EpCam and HIF1a]


title: Tumor-plate10-67 [EpCam and HIF1a]


title: Tumor-plate10-66 [EpCam and HIF1a]


title: Tumor-plate10-63 [EpCam and HIF1a]


title: Tumor-plate10-64 [EpCam and HIF1a]


title: Tumor-plate10-62 [EpCam and HIF1a]


title: Tumor-plate10-65 [EpCam and HIF1a]


title: Tumor-plate10-81 [EpCam and HIF1a]


title: Tumor-plate10-6 [EpCam and HIF1a]


title: Tumor-plate10-59 [EpCam and HIF1a]


title: Tumor-plate10-60 [EpCam and HIF1a]


title: Tumor-plate10-57 [EpCam and HIF1a]


title: Tumor-plate10-56 [EpCam and HIF1a]


title: Tumor-plate10-50 [EpCam and HIF1a]


title: Tumor-plate10-49 [EpCam and HIF1a]


title: Tumor-plate10-53 [EpCam and HIF1a]


title: Tumor-plate10-48 [EpCam and HIF1a]


title: Tumor-plate10-61 [EpCam and HIF1a]


title: Tumor-plate10-52 [EpCam and HIF1a]


title: Tumor-plate10-51 [EpCam and HIF1a]


title: Tumor-plate10-54 [EpCam and HIF1a]