title: Ubc9Tg 45mins ischemia 3hrs reperfusion rep4

description: Ubc9Tg 45mins ischemia 3hrs reperfusion

value: 45mins ischemia 3hrs reperfusion


title: Pancreatic Cancer [PC0753]

status: Public on Nov 06 2018

type: RNA

city: Kanagawa

first: Satoko

last: Takizawa

address: kamakurashi

country: Japan

institute: Toray industries inc

description: miRNA

hyb_protocol: Hybridized for 16 h at 32 ºC with rotary shaker (250 rpm). Hybridization buffer and washing protocol were attached in the 3D-Gene® miRNA oligo chip kit.

scan_protocol: 3D-Gene® Scanner (Toray Industries, Inc.) was used for scanning. Images were quantified using Extraction version 2.0.0.6 (Toray Industries, Inc.).

data_processing: The presence of miRNA was determined based on a corresponding microarray signal of greater than [the mean + 2 × standard deviation] of the negative controls signal, of which the top and bottom ranked ones by signal intensity were removed. Once a miRNA was considered present, the mean signal of the negative controls of which the top and bottom 5% ranked by signal intensity were removed was subtracted from the miRNA signal. When the signal value was negative (or undetected) after background subtraction, the value was replaced by 0.1 on a base 2 logarithm scale.

molecule: total RNA

organism: Homo sapiens

source_name: Serum

label_protocol: miRNA was labeled using 3D-Gene® miRNA Labeling kit in accordance with the manufacturer's instructions.

extract_protocol: Total RNA was extracted each from 300uL serum samples using 3D-Gene® RNA extraction reagent from liquid sample kit (Toray Industries, Inc.).

tag: tissue

value: Serum

tag: Sex

value: Male

tag: age

tag: disease status

value: Pancreatic Cancer

tag: pathological tstage

value: uncertain

tag: pathological grade


title: Prostate Cancer [PR0410]

description: miRNA

value: Prostate Cancer


title: Pancreatic Cancer [PC0734]

description: miRNA

value: Female


title: Pancreatic Cancer [PC0770]

description: miRNA


title: Pancreatic Cancer [PC0696]

description: miRNA


title: Pancreatic Cancer [PC0728]

description: miRNA


title: Pancreatic Cancer [PC0675]

description: miRNA


title: Pancreatic Cancer [PC0853]

description: miRNA


title: Pancreatic Cancer [PC0663]

description: miRNA


title: Pancreatic Cancer [PC0545]

description: miRNA


title: Pancreatic Cancer [PC0567]

description: miRNA


title: Pancreatic Cancer [PC0551]

description: miRNA


title: Pancreatic Cancer [PC0593]

description: miRNA


title: Pancreatic Cancer [PC0525]

description: miRNA


title: Pancreatic Cancer [PC0535]

description: miRNA


title: Pancreatic Cancer [PC0620]

description: miRNA


title: Pancreatic Cancer [PC0483]

description: miRNA


title: Pancreatic Cancer [PC0309]

description: miRNA


title: Pancreatic Cancer [PC0583]

description: miRNA


title: Pancreatic Cancer [PC0618]

description: miRNA


title: Pancreatic Cancer [PC0555]

description: miRNA


title: Pancreatic Cancer [PC0604]

description: miRNA


title: Pancreatic Cancer [PC0348]

description: miRNA


title: Pancreatic Cancer [PC0478]

description: miRNA


title: Pancreatic Cancer [PC0331]

description: miRNA


title: Pancreatic Cancer [PC0366]

description: miRNA


title: Pancreatic Cancer [PC0470]

description: miRNA


title: Pancreatic Cancer [PC0475]

description: miRNA


title: Pancreatic Cancer [PC0275]

description: miRNA


title: Pancreatic Cancer [PC0393]

description: miRNA


title: Pancreatic Cancer [PC0273]

description: miRNA


title: Pancreatic Cancer [PC0271]

description: miRNA


title: Pancreatic Cancer [PC0445]

description: miRNA


title: Pancreatic Cancer [PC0286]

description: miRNA


title: Pancreatic Cancer [PC0265]

description: miRNA


title: Ovarian Cancer [OV0430]

description: miRNA

value: Ovarian Cancer


title: Pancreatic Cancer [PC0256]

description: miRNA


title: Pancreatic Cancer [PC0235]

description: miRNA


title: Ovarian Cancer [OV0440]

description: miRNA


title: Ovarian Cancer [OV0410]

description: miRNA


title: Ovarian Cancer [OV0411]

description: miRNA


title: Ovarian Cancer [OV0402]

description: miRNA


title: Ovarian Cancer [OV0399]

description: miRNA


title: Ovarian Cancer [OV0395]

description: miRNA


title: Ovarian Cancer [OV0350]

description: miRNA


title: Ovarian Cancer [OV0269]

description: miRNA


title: Ovarian Cancer [OV0262]

description: miRNA


title: Ovarian Cancer [OV0324]

description: miRNA


title: Ovarian Cancer [OV0304]

description: miRNA


title: Ovarian Cancer [OV0332]

description: miRNA


title: Ovarian Cancer [OV0254]

description: miRNA


title: Ovarian Cancer [OV0346]

description: miRNA


title: Ovarian Cancer [OV0225]

description: miRNA


title: Ovarian Cancer [OV0320]

description: miRNA


title: Ovarian Cancer [OV0295]

description: miRNA


title: Ovarian Cancer [OV0170]

description: miRNA


title: Ovarian Cancer [OV0168]

description: miRNA


title: Ovarian Cancer [OV0222]

description: miRNA


title: Ovarian Cancer [OV0181]

description: miRNA


title: Ovarian Cancer [OV0198]

description: miRNA


title: Ovarian Cancer [OV0176]

description: miRNA


title: Ovarian Cancer [OV0145]

description: miRNA


title: Ovarian Cancer [OV0140]

description: miRNA


title: Ovarian Cancer [OV0158]

description: miRNA


title: Ovarian Cancer [OV0161]

description: miRNA


title: Ovarian Cancer [OV0121]

description: miRNA


title: Ovarian Cancer [OV0114]

description: miRNA


title: Ovarian Cancer [OV0135]

description: miRNA


title: Ovarian Cancer [OV0106]

description: miRNA


title: Ovarian Cancer [OV0123]

description: miRNA


title: Ovarian Cancer [OV0211]

description: miRNA


title: Ovarian Cancer [OV0102]

description: miRNA


title: Ovarian Cancer [OV0152]

description: miRNA


title: Ovarian Cancer [OV0090]

description: miRNA


title: Ovarian Cancer [OV0083]

description: miRNA


title: Lung Cancer [LK1634]

description: miRNA

value: Lung Cancer


title: Ovarian Cancer [OV0081]

description: miRNA


title: Lung Cancer [LK1407]

description: miRNA


title: Ovarian Cancer [OV0075]

description: miRNA


title: Lung Cancer [LK0558]

description: miRNA


title: Lung Cancer [LK1265]

description: miRNA


title: Lung Cancer [LK1615]

description: miRNA


title: Lung Cancer [LK0674]

description: miRNA


title: Lung Cancer [LK0600]

description: miRNA


title: Lung Cancer [LK1606]

description: miRNA


title: Lung Cancer [LK1250]

description: miRNA


title: Lung Cancer [LK0534]

description: miRNA


title: Lung Cancer [LK1233]

description: miRNA


title: Lung Cancer [LK1564]

description: miRNA


title: Lung Cancer [LK1069]

description: miRNA


title: Lung Cancer [LK0688]

description: miRNA


title: Lung Cancer [LK1543]

description: miRNA


title: Lung Cancer [LK1103]

description: miRNA


title: Lung Cancer [LK0531]

description: miRNA


title: Lung Cancer [LK1042]

description: miRNA


title: Lung Cancer [LK0523]

description: miRNA


title: Lung Cancer [LK1054]

description: miRNA


title: Lung Cancer [LK0508]

description: miRNA


title: Lung Cancer [LK0466]

description: miRNA


title: Lung Cancer [LK1154]

description: miRNA


title: Lung Cancer [LK0949]

description: miRNA


title: Lung Cancer [LK0452]

description: miRNA


title: Lung Cancer [LK0878]

description: miRNA


title: Lung Cancer [LK0304]

description: miRNA


title: Lung Cancer [LK0247]

description: miRNA


title: Lung Cancer [LK0259]

description: miRNA


title: Lung Cancer [LK0118]

description: miRNA


title: Lung Cancer [LK0190]

description: miRNA


title: Lung Cancer [LK0108]

description: miRNA


title: Lung Cancer [LK0347]

description: miRNA


title: Lung Cancer [LK0201]

description: miRNA


title: Lung Cancer [LK0102]

description: miRNA


title: Lung Cancer [LK0120]

description: miRNA


title: Lung Cancer [LK0109]

description: miRNA


title: Hepatocellular Carcinoma [HC0289]

description: miRNA

value: Hepatocellular Carcinoma


title: Hepatocellular Carcinoma [HC0336]

description: miRNA


title: Lung Cancer [LK0024]

description: miRNA


title: Hepatocellular Carcinoma [HC0276]

description: miRNA


title: Hepatocellular Carcinoma [HC0302]

description: miRNA


title: Hepatocellular Carcinoma [HC0337]

description: miRNA


title: Hepatocellular Carcinoma [HC0269]

description: miRNA


title: Hepatocellular Carcinoma [HC0244]

description: miRNA


title: Lung Cancer [LK0005]

description: miRNA


title: Hepatocellular Carcinoma [HC0274]

description: miRNA


title: Hepatocellular Carcinoma [HC0307]

description: miRNA


title: Hepatocellular Carcinoma [HC0263]

description: miRNA


title: Hepatocellular Carcinoma [HC0259]

description: miRNA


title: Hepatocellular Carcinoma [HC0243]

description: miRNA


title: Lung Cancer [LK0209]

description: miRNA


title: Hepatocellular Carcinoma [HC0220]

description: miRNA


title: Hepatocellular Carcinoma [HC0207]

description: miRNA


title: Hepatocellular Carcinoma [HC0185]

description: miRNA


title: Hepatocellular Carcinoma [HC0208]

description: miRNA


title: Hepatocellular Carcinoma [HC0198]

description: miRNA


title: Hepatocellular Carcinoma [HC0179]

description: miRNA


title: Hepatocellular Carcinoma [HC0171]

description: miRNA


title: Hepatocellular Carcinoma [HC0130]

description: miRNA


title: Hepatocellular Carcinoma [HC0103]

description: miRNA


title: Hepatocellular Carcinoma [HC0107]

description: miRNA


title: Hepatocellular Carcinoma [HC0120]

description: miRNA


title: Hepatocellular Carcinoma [HC0178]

description: miRNA


title: Hepatocellular Carcinoma [HC0112]

description: miRNA


title: Hepatocellular Carcinoma [HC0159]

description: miRNA


title: Hepatocellular Carcinoma [HC0158]

description: miRNA


title: Hepatocellular Carcinoma [HC0138]

description: miRNA


title: Hepatocellular Carcinoma [HC0118]

description: miRNA


title: Hepatocellular Carcinoma [HC0133]

description: miRNA


title: Hepatocellular Carcinoma [HC0101]

description: miRNA


title: Hepatocellular Carcinoma [HC0092]

description: miRNA


title: Glioma [GL0221]

description: miRNA

value: Glioma


title: Hepatocellular Carcinoma [HC0084]

description: miRNA


title: Hepatocellular Carcinoma [HC0076]

description: miRNA


title: Hepatocellular Carcinoma [HC0057]

description: miRNA


title: Hepatocellular Carcinoma [HC0065]

description: miRNA


title: Glioma [GL0198]

description: miRNA


title: Hepatocellular Carcinoma [HC0328]

description: miRNA


title: Hepatocellular Carcinoma [HC0070]

description: miRNA


title: Hepatocellular Carcinoma [HC0054]

description: miRNA


title: Glioma [GL0189]

description: miRNA


title: Hepatocellular Carcinoma [HC0147]

description: miRNA


title: Glioma [GL0175]

description: miRNA


title: Glioma [GL0173]

description: miRNA


title: Glioma [GL0171]

description: miRNA


title: Glioma [GL0180]

description: miRNA


title: Glioma [GL0146]

description: miRNA


title: Glioma [GL0142]

description: miRNA


title: Glioma [GL0135]

description: miRNA


title: Glioma [GL0100]

description: miRNA


title: Glioma [GL0090]

description: miRNA


title: Glioma [GL0099]

description: miRNA


title: Glioma [GL0124]

description: miRNA


title: Glioma [GL0115]

description: miRNA


title: Glioma [GL0091]

description: miRNA


title: Glioma [GL0084]

description: miRNA


title: Glioma [GL0078]

description: miRNA


title: Glioma [GL0069]

description: miRNA


title: Glioma [GL0074]

description: miRNA


title: Glioma [GL0059]

description: miRNA


title: Glioma [GL0040]

description: miRNA


title: Glioma [GL0061]

description: miRNA


title: Glioma [GL0047]

description: miRNA


title: Glioma [GL0046]

description: miRNA


title: Glioma [GL0055]

description: miRNA


title: Glioma [GL0128]

description: miRNA


title: Glioma [GL0133]

description: miRNA


title: Glioma [GL0034]

description: miRNA


title: Glioma [GL0025]

description: miRNA


title: Glioma [GL0012]

description: miRNA


title: Glioma [GL0132]

description: miRNA


title: Glioma [GL0005]

description: miRNA


title: Glioma [GL0029]

description: miRNA


title: Glioma [GL0018]

description: miRNA


title: Glioma [GL0098]

description: miRNA


title: Glioma [GL0044]

description: miRNA


title: Glioma [GL0003]

description: miRNA


title: Gastric Cancer [GC1373]

description: miRNA

value: Gastric Cancer


title: Gastric Cancer [GC1333]

description: miRNA


title: Gastric Cancer [GC1433]

description: miRNA


title: Gastric Cancer [GC1347]

description: miRNA


title: Gastric Cancer [GC1444]

description: miRNA


title: Glioma [GL0014]

description: miRNA


title: Gastric Cancer [GC1010]

description: miRNA


title: Gastric Cancer [GC0986]

description: miRNA


title: Gastric Cancer [GC1106]

description: miRNA


title: Gastric Cancer [GC1260]

description: miRNA


title: Gastric Cancer [GC0902]

description: miRNA


title: Gastric Cancer [GC1092]

description: miRNA


title: Gastric Cancer [GC0958]

description: miRNA


title: Gastric Cancer [GC1245]

description: miRNA


title: Gastric Cancer [GC0947]

description: miRNA


title: Gastric Cancer [GC0929]

description: miRNA


title: Glioma [GL0001]

description: miRNA


title: Gastric Cancer [GC0873]

description: miRNA


title: Gastric Cancer [GC0724]

description: miRNA


title: Gastric Cancer [GC0934]

description: miRNA


title: Gastric Cancer [GC0746]

description: miRNA


title: Gastric Cancer [GC0671]

description: miRNA


title: Gastric Cancer [GC1447]

description: miRNA


title: Gastric Cancer [GC0659]

description: miRNA


title: Gastric Cancer [GC0835]

description: miRNA


title: Gastric Cancer [GC0792]

description: miRNA


title: Gastric Cancer [GC0602]

description: miRNA


title: Gastric Cancer [GC0976]

description: miRNA


title: Gastric Cancer [GC0565]

description: miRNA


title: Gastric Cancer [GC0509]

description: miRNA


title: Gastric Cancer [GC0469]

description: miRNA


title: Gastric Cancer [GC0461]

description: miRNA


title: Gastric Cancer [GC0556]

description: miRNA


title: Gastric Cancer [GC0391]

description: miRNA


title: Gastric Cancer [GC0397]

description: miRNA


title: Gastric Cancer [GC0427]

description: miRNA


title: Gastric Cancer [GC0415]

description: miRNA


title: Gastric Cancer [GC0340]

description: miRNA


title: Gastric Cancer [GC0373]

description: miRNA


title: Esophageal Cancer [EC0595]

description: miRNA

value: Esophageal Cancer


title: Gastric Cancer [GC0354]

description: miRNA


title: Esophageal Cancer [EC0630]

description: miRNA


title: Esophageal Cancer [EC0586]

description: miRNA


title: Gastric Cancer [GC0557]

description: miRNA


title: Esophageal Cancer [EC0587]

description: miRNA


title: Esophageal Cancer [EC0567]

description: miRNA


title: Esophageal Cancer [EC0570]

description: miRNA


title: Esophageal Cancer [EC0581]

description: miRNA


title: Esophageal Cancer [EC0546]

description: miRNA


title: Esophageal Cancer [EC0526]

description: miRNA


title: Esophageal Cancer [EC0450]

description: miRNA


title: Esophageal Cancer [EC0523]

description: miRNA


title: Esophageal Cancer [EC0437]

description: miRNA


title: Esophageal Cancer [EC0490]

description: miRNA


title: Gastric Cancer [GC0384]

description: miRNA


title: Esophageal Cancer [EC0421]

description: miRNA


title: Esophageal Cancer [EC0311]

description: miRNA


title: Esophageal Cancer [EC0320]

description: miRNA


title: Esophageal Cancer [EC0292]

description: miRNA


title: Esophageal Cancer [EC0531]

description: miRNA


title: Esophageal Cancer [EC0235]

description: miRNA


title: Esophageal Cancer [EC0241]

description: miRNA


title: Esophageal Cancer [EC0258]

description: miRNA


title: Esophageal Cancer [EC0146]

description: miRNA


title: Esophageal Cancer [EC0262]

description: miRNA


title: Esophageal Cancer [EC0228]

description: miRNA


title: Esophageal Cancer [EC0257]

description: miRNA


title: Esophageal Cancer [EC0264]

description: miRNA


title: Esophageal Cancer [EC0140]

description: miRNA


title: Esophageal Cancer [EC0279]

description: miRNA


title: Esophageal Cancer [EC0105]

description: miRNA


title: Esophageal Cancer [EC0121]

description: miRNA


title: Esophageal Cancer [EC0091]

description: miRNA


title: Esophageal Cancer [EC0052]

description: miRNA


title: Esophageal Cancer [EC0042]

description: miRNA


title: Esophageal Cancer [EC0289]

description: miRNA


title: Esophageal Cancer [EC0020]

description: miRNA


title: Colorectal Cancer [CC1552]

description: miRNA

value: Colorectal Cancer


title: Colorectal Cancer [CC1423]

description: miRNA


title: Esophageal Cancer [EC0032]

description: miRNA


title: Colorectal Cancer [CC1371]

description: miRNA


title: Colorectal Cancer [CC1318]

description: miRNA


title: Colorectal Cancer [CC1314]

description: miRNA


title: Colorectal Cancer [CC1533]

description: miRNA


title: Colorectal Cancer [CC1224]

description: miRNA


title: Colorectal Cancer [CC1273]

description: miRNA


title: Colorectal Cancer [CC1262]

description: miRNA


title: Colorectal Cancer [CC1021]

description: miRNA


title: Colorectal Cancer [CC1084]

description: miRNA


title: Colorectal Cancer [CC1192]

description: miRNA


title: Esophageal Cancer [EC0057]

description: miRNA


title: Colorectal Cancer [CC0890]

description: miRNA


title: Esophageal Cancer [EC0103]

description: miRNA


title: Esophageal Cancer [EC0038]

description: miRNA


title: Colorectal Cancer [CC0783]

description: miRNA


title: Colorectal Cancer [CC1298]

description: miRNA


title: Colorectal Cancer [CC0736]

description: miRNA


title: Colorectal Cancer [CC0781]

description: miRNA


title: Colorectal Cancer [CC0758]

description: miRNA


title: Colorectal Cancer [CC0695]

description: miRNA


title: Colorectal Cancer [CC0596]

description: miRNA


title: Colorectal Cancer [CC0424]

description: miRNA


title: Colorectal Cancer [CC1205]

description: miRNA


title: Colorectal Cancer [CC0502]

description: miRNA


title: Colorectal Cancer [CC1150]

description: miRNA


title: Colorectal Cancer [CC0460]

description: miRNA


title: Colorectal Cancer [CC0603]

description: miRNA


title: Colorectal Cancer [CC0435]

description: miRNA


title: Colorectal Cancer [CC0530]

description: miRNA


title: Colorectal Cancer [CC0498]

description: miRNA


title: Colorectal Cancer [CC0313]

description: miRNA


title: Colorectal Cancer [CC0400]

description: miRNA


title: Colorectal Cancer [CC0304]

description: miRNA


title: Colorectal Cancer [CC0294]

description: miRNA


title: Colorectal Cancer [CC0387]

description: miRNA


title: Colorectal Cancer [CC0296]

description: miRNA


title: Colorectal Cancer [CC0861]

description: miRNA


title: Colorectal Cancer [CC0249]

description: miRNA


title: Colorectal Cancer [CC0235]

description: miRNA


title: Biliary Tract Cancer [BT0398]

description: miRNA

value: Biliary Tract Cancer


title: Biliary Tract Cancer [BT0392]

description: miRNA


title: Biliary Tract Cancer [BT0388]

description: miRNA


title: Biliary Tract Cancer [BT0382]

description: miRNA


title: Biliary Tract Cancer [BT0383]

description: miRNA


title: Biliary Tract Cancer [BT0376]

description: miRNA


title: Biliary Tract Cancer [BT0353]

description: miRNA


title: Biliary Tract Cancer [BT0338]

description: miRNA


title: Biliary Tract Cancer [BT0324]

description: miRNA


title: Biliary Tract Cancer [BT0273]

description: miRNA


title: Biliary Tract Cancer [BT0260]

description: miRNA


title: Biliary Tract Cancer [BT0307]

description: miRNA


title: Biliary Tract Cancer [BT0277]

description: miRNA


title: Biliary Tract Cancer [BT0264]

description: miRNA


title: Biliary Tract Cancer [BT0256]

description: miRNA


title: Biliary Tract Cancer [BT0316]

description: miRNA


title: Biliary Tract Cancer [BT0231]

description: miRNA


title: Biliary Tract Cancer [BT0257]

description: miRNA


title: Biliary Tract Cancer [BT0247]

description: miRNA


title: Biliary Tract Cancer [BT0235]

description: miRNA


title: Biliary Tract Cancer [BT0201]

description: miRNA


title: Biliary Tract Cancer [BT0278]

description: miRNA


title: Biliary Tract Cancer [BT0194]

description: miRNA


title: Esophageal Cancer [EC0035]

description: miRNA


title: Biliary Tract Cancer [BT0185]

description: miRNA


title: Biliary Tract Cancer [BT0184]

description: miRNA


title: Biliary Tract Cancer [BT0186]

description: miRNA


title: Biliary Tract Cancer [BT0142]

description: miRNA


title: Biliary Tract Cancer [BT0297]

description: miRNA


title: Biliary Tract Cancer [BT0113]

description: miRNA


title: Biliary Tract Cancer [BT0241]

description: miRNA


title: Biliary Tract Cancer [BT0149]

description: miRNA


title: Biliary Tract Cancer [BT0210]

description: miRNA


title: Biliary Tract Cancer [BT0098]

description: miRNA


title: Biliary Tract Cancer [BT0096]

description: miRNA


title: Biliary Tract Cancer [BT0193]

description: miRNA


title: Biliary Tract Cancer [BT0082]

description: miRNA


title: Biliary Tract Cancer [BT0048]

description: miRNA


title: Breast Cancer [BC1607]

description: miRNA

value: Breast Cancer


title: Breast Cancer [BC1552]

description: miRNA


title: Breast Cancer [BC1581]

description: miRNA


title: Biliary Tract Cancer [BT0052]

description: miRNA


title: Breast Cancer [BC1410]

description: miRNA


title: Breast Cancer [BC1456]

description: miRNA


title: Breast Cancer [BC1540]

description: miRNA


title: Biliary Tract Cancer [BT0045]

description: miRNA


title: Breast Cancer [BC1357]

description: miRNA


title: Biliary Tract Cancer [BT0125]

description: miRNA


title: Breast Cancer [BC1376]

description: miRNA


title: Breast Cancer [BC1353]

description: miRNA


title: Breast Cancer [BC1352]

description: miRNA


title: Breast Cancer [BC1596]

description: miRNA


title: Breast Cancer [BC1350]

description: miRNA


title: Breast Cancer [BC1345]

description: miRNA


title: Breast Cancer [BC1349]

description: miRNA


title: Breast Cancer [BC1341]

description: miRNA


title: Breast Cancer [BC1335]

description: miRNA


title: Breast Cancer [BC1351]

description: miRNA


title: Breast Cancer [BC1334]

description: miRNA


title: Breast Cancer [BC1331]

description: miRNA


title: Breast Cancer [BC1330]

description: miRNA


title: Breast Cancer [BC1304]

description: miRNA


title: Breast Cancer [BC1313]

description: miRNA


title: Breast Cancer [BC1434]

description: miRNA


title: Breast Cancer [BC1301]

description: miRNA


title: Breast Cancer [BC1298]

description: miRNA


title: Breast Cancer [BC1293]

description: miRNA


title: Breast Cancer [BC1292]

description: miRNA


title: Breast Cancer [BC1248]

description: miRNA


title: Breast Cancer [BC1222]

description: miRNA


title: Breast Cancer [BC1179]

description: miRNA


title: Breast Cancer [BC1261]

description: miRNA


title: Breast Cancer [BC1280]

description: miRNA


title: Breast Cancer [BC1277]

description: miRNA


title: Breast Cancer [BC1288]

description: miRNA


title: Breast Cancer [BC1279]

description: miRNA


title: Breast Cancer [BC1138]

description: miRNA


title: Non-cancer control [XB0930]

description: miRNA

value: Non-cancer control


title: Breast Cancer [BC1068]

description: miRNA


title: Breast Cancer [BC1056]

description: miRNA


title: Non-cancer control [XB0989]

description: miRNA


title: Non-cancer control [XB0442]

description: miRNA


title: Non-cancer control [XB0368]

description: miRNA


title: Colorectal Cancer [CC0981]

description: miRNA


title: Non-cancer control [XB0566]

description: miRNA


title: Breast Cancer [BC1289]

description: miRNA


title: Non-cancer control [XB0250]

description: miRNA


title: Non-cancer control [XB0781]

description: miRNA


title: Non-cancer control [XB0195]

description: miRNA


title: Non-cancer control [XB0568]

description: miRNA


title: Non-cancer control [XA0995]

description: miRNA


title: Non-cancer control [XB0703]

description: miRNA


title: Breast Cancer [BC1057]

description: miRNA


title: Non-cancer control [XB0858]

description: miRNA


title: Non-cancer control [XA0965]

description: miRNA


title: Non-cancer control [XA0644]

description: miRNA


title: Non-cancer control [XA0647]

description: miRNA


title: Non-cancer control [XA0685]

description: miRNA


title: Non-cancer control [XA0557]

description: miRNA


title: Non-cancer control [XA0520]

description: miRNA


title: Non-cancer control [XA0716]

description: miRNA


title: Non-cancer control [XA0471]

description: miRNA


title: Non-cancer control [XA0343]

description: miRNA


title: Non-cancer control [XB0300]

description: miRNA


title: Non-cancer control [XA0100]

description: miRNA


title: Non-cancer control [XA0350]

description: miRNA


title: Non-cancer control [NB15122505]

description: miRNA


title: Non-cancer control [NB16030301]

description: miRNA


title: Non-cancer control [NB15070203]

description: miRNA


title: Non-cancer control [NB14121901]

description: miRNA


title: Non-cancer control [NB15072706]

description: miRNA


title: Non-cancer control [NB15122201]

description: miRNA


title: Non-cancer control [NB15111801]

description: miRNA


title: Non-cancer control [NB14081101]

description: miRNA


title: Non-cancer control [NB14102003]

description: miRNA


title: Non-cancer control [NB13120602]

description: miRNA


title: Colorectal Cancer [CC1193]

description: miRNA


title: Non-cancer control [NB14031302]

description: miRNA


title: Non-cancer control [NB13080201]

description: miRNA


title: Non-cancer control [XA0743]

description: miRNA


title: Non-cancer control [NB13061003]

description: miRNA


title: Non-cancer control [NB13032501]

description: miRNA


title: Non-cancer control [NB13050803]

description: miRNA


title: Non-cancer control [NB12071901]

description: miRNA


title: Non-cancer control [M11120803]

description: miRNA


title: Non-cancer control [NB12022204]

description: miRNA


title: Non-cancer control [M11091302]

description: miRNA


title: Non-cancer control [M10121602]

description: miRNA


title: Non-cancer control [M11012502]

description: miRNA


title: Non-cancer control [CHC785]

description: miRNA


title: Non-cancer control [NB15120103]

description: miRNA


title: Non-cancer control [M11110203]

description: miRNA


title: Non-cancer control [M11061002]

description: miRNA


title: Non-cancer control [CHC797]

description: miRNA


title: Non-cancer control [NB14071601]

description: miRNA


title: Non-cancer control [NB15122506]

description: miRNA


title: Non-cancer control [NB15122204]

description: miRNA


title: Non-cancer control [NB14030504]

description: miRNA


title: Non-cancer control [NB15070702]

description: miRNA


title: Non-cancer control [NB14110603]

description: miRNA


title: Non-cancer control [NB13101502]

description: miRNA


title: Non-cancer control [NB13052306]

description: miRNA


title: Non-cancer control [NB12111401]

description: miRNA


title: Non-cancer control [NB13041504]

description: miRNA


title: Non-cancer control [NB12041901]

description: miRNA


title: Non-cancer control [NB13053103]

description: miRNA


title: Non-cancer control [NB15052601]

description: miRNA


title: Non-cancer control [M11122103]

description: miRNA


title: Non-cancer control [M10121601]

description: miRNA


title: Non-cancer control [M11111103]

description: miRNA


title: Non-cancer control [NB13062502]

description: miRNA


title: Non-cancer control [M10122802]

description: miRNA


title: Non-cancer control [M11042602]

description: miRNA


title: Non-cancer control [M11101105]

description: miRNA


title: Non-cancer control [NB14091901]

description: miRNA


title: Non-cancer control [XB0794]

description: miRNA


title: Non-cancer control [NB13052101]

description: miRNA


title: Non-cancer control [XB0706]

description: miRNA


title: Non-cancer control [XB0614]

description: miRNA


title: Non-cancer control [XB0863]

description: miRNA


title: Non-cancer control [XA0044]

description: miRNA


title: Non-cancer control [XB0567]

description: miRNA


title: Non-cancer control [XB0938]

description: miRNA


title: Non-cancer control [XB0196]

description: miRNA


title: Non-cancer control [XB0282]

description: miRNA


title: Non-cancer control [XA0986]

description: miRNA


title: Non-cancer control [XB0325]

description: miRNA


title: Non-cancer control [XB0392]

description: miRNA


title: Non-cancer control [M11070402]

description: miRNA


title: Non-cancer control [XA0833]

description: miRNA


title: Non-cancer control [XA0730]

description: miRNA


title: Non-cancer control [XA0687]

description: miRNA


title: Non-cancer control [XB0459]

description: miRNA


title: Non-cancer control [XA0646]

description: miRNA


title: Non-cancer control [XA0670]

description: miRNA


title: Non-cancer control [XA0523]

description: miRNA


title: Non-cancer control [XA0509]

description: miRNA


title: Non-cancer control [NB15101502]

description: miRNA


title: Non-cancer control [XA0308]

description: miRNA


title: Non-cancer control [XA0593]

description: miRNA


title: Non-cancer control [XA0368]

description: miRNA


title: Bladder Cancer [BL0508]

description: miRNA

value: Bladder Cancer

value: high


title: Bladder Cancer [BL0511]

description: miRNA

value: Serum

value: Male


title: Bladder Cancer [BL0514]

description: miRNA


title: Bladder Cancer [BL0512]

description: miRNA


title: Bladder Cancer [BL0513]

description: miRNA


title: Non-cancer control [XA0300]

description: miRNA


title: Bladder Cancer [BL0507]

description: miRNA


title: Bladder Cancer [BL0509]

description: miRNA

value: low


title: Non-cancer control [XB0025]

description: miRNA


title: Bladder Cancer [BL0510]

description: miRNA


title: Bladder Cancer [BL0500]

description: miRNA


title: Bladder Cancer [BL0502]

description: miRNA


title: Bladder Cancer [BL0504]

description: miRNA


title: Bladder Cancer [BL0501]

description: miRNA


title: Bladder Cancer [BL0489]

description: miRNA


title: Bladder Cancer [BL0503]

description: miRNA


title: Bladder Cancer [BL0494]

description: miRNA


title: Bladder Cancer [BL0488]

description: miRNA


title: Bladder Cancer [BL0497]

description: miRNA


title: Bladder Cancer [BL0496]

description: miRNA


title: Bladder Cancer [BL0505]

description: miRNA


title: Bladder Cancer [BL0499]

description: miRNA


title: Bladder Cancer [BL0485]

description: miRNA


title: Bladder Cancer [BL0486]

description: miRNA


title: Bladder Cancer [BL0487]

description: miRNA


title: Bladder Cancer [BL0498]

description: miRNA


title: Bladder Cancer [BL0506]

description: miRNA


title: Bladder Cancer [BL0484]

description: miRNA


title: Bladder Cancer [BL0479]

description: miRNA


title: Bladder Cancer [BL0481]

description: miRNA


title: Bladder Cancer [BL0480]

description: miRNA


title: Bladder Cancer [BL0493]

description: miRNA


title: Bladder Cancer [BL0483]

description: miRNA


title: Bladder Cancer [BL0495]

description: miRNA


title: Bladder Cancer [BL0478]

description: miRNA


title: Bladder Cancer [BL0477]

description: miRNA


title: Bladder Cancer [BL0492]

description: miRNA


title: Bladder Cancer [BL0475]

description: miRNA


title: Bladder Cancer [BL0471]

description: miRNA


title: Bladder Cancer [BL0465]

description: miRNA


title: Bladder Cancer [BL0466]

description: miRNA


title: Bladder Cancer [BL0470]

description: miRNA


title: Bladder Cancer [BL0473]

description: miRNA


title: Bladder Cancer [BL0474]

description: miRNA


title: Bladder Cancer [BL0468]

description: miRNA


title: Bladder Cancer [BL0467]

description: miRNA


title: Bladder Cancer [BL0476]

description: miRNA


title: Bladder Cancer [BL0463]

description: miRNA


title: Bladder Cancer [BL0460]

description: miRNA


title: Bladder Cancer [BL0461]

description: miRNA


title: Bladder Cancer [BL0464]

description: miRNA


title: Bladder Cancer [BL0456]

description: miRNA


title: Bladder Cancer [BL0459]

description: miRNA


title: Bladder Cancer [BL0458]

description: miRNA


title: Bladder Cancer [BL0457]

description: miRNA


title: Bladder Cancer [BL0455]

description: miRNA


title: Bladder Cancer [BL0454]

description: miRNA


title: Bladder Cancer [BL0452]

description: miRNA


title: Bladder Cancer [BL0449]

description: miRNA


title: Bladder Cancer [BL0451]

description: miRNA


title: Bladder Cancer [BL0448]

description: miRNA


title: Bladder Cancer [BL0446]

description: miRNA


title: Bladder Cancer [BL0442]

description: miRNA


title: Bladder Cancer [BL0445]

description: miRNA


title: Bladder Cancer [BL0443]

description: miRNA


title: Bladder Cancer [BL0441]

description: miRNA


title: Bladder Cancer [BL0440]

description: miRNA


title: Bladder Cancer [BL0438]

description: miRNA


title: Bladder Cancer [BL0437]

description: miRNA


title: Bladder Cancer [BL0433]

description: miRNA


title: Bladder Cancer [BL0435]

description: miRNA


title: Bladder Cancer [BL0432]

description: miRNA


title: Bladder Cancer [BL0434]

description: miRNA


title: Bladder Cancer [BL0431]

description: miRNA


title: Bladder Cancer [BL0426]

description: miRNA


title: Bladder Cancer [BL0450]

description: miRNA


title: Bladder Cancer [BL0428]

description: miRNA


title: Bladder Cancer [BL0423]

description: miRNA


title: Bladder Cancer [BL0420]

description: miRNA


title: Bladder Cancer [BL0424]

description: miRNA


title: Bladder Cancer [BL0422]

description: miRNA


title: Bladder Cancer [BL0416]

description: miRNA


title: Bladder Cancer [BL0417]

description: miRNA


title: Bladder Cancer [BL0429]

description: miRNA


title: Bladder Cancer [BL0419]

description: miRNA


title: Bladder Cancer [BL0418]

description: miRNA


title: Bladder Cancer [BL0414]

description: miRNA


title: Bladder Cancer [BL0415]

description: miRNA


title: Bladder Cancer [BL0436]

description: miRNA


title: Bladder Cancer [BL0409]

description: miRNA


title: Bladder Cancer [BL0413]

description: miRNA


title: Bladder Cancer [BL0407]

description: miRNA


title: Bladder Cancer [BL0425]

description: miRNA


title: Bladder Cancer [BL0411]

description: miRNA


title: Bladder Cancer [BL0408]

description: miRNA


title: Bladder Cancer [BL0404]

description: miRNA


title: Bladder Cancer [BL0406]

description: miRNA


title: Bladder Cancer [BL0402]

description: miRNA


title: Bladder Cancer [BL0401]

description: miRNA


title: Bladder Cancer [BL0399]

description: miRNA


title: Bladder Cancer [BL0396]

description: miRNA


title: Bladder Cancer [BL0394]

description: miRNA


title: Bladder Cancer [BL0410]

description: miRNA


title: Bladder Cancer [BL0392]

description: miRNA


title: Bladder Cancer [BL0391]

description: miRNA


title: Bladder Cancer [BL0386]

description: miRNA


title: Bladder Cancer [BL0389]

description: miRNA


title: Bladder Cancer [BL0387]

description: miRNA


title: Bladder Cancer [BL0385]

description: miRNA


title: Bladder Cancer [BL0384]

description: miRNA


title: Bladder Cancer [BL0382]

description: miRNA


title: Bladder Cancer [BL0400]

description: miRNA


title: Bladder Cancer [BL0379]

description: miRNA


title: Bladder Cancer [BL0374]

description: miRNA


title: Bladder Cancer [BL0377]

description: miRNA


title: Bladder Cancer [BL0376]

description: miRNA


title: Bladder Cancer [BL0375]

description: miRNA


title: Bladder Cancer [BL0380]

description: miRNA


title: Bladder Cancer [BL0390]

description: miRNA


title: Bladder Cancer [BL0367]

description: miRNA


title: Bladder Cancer [BL0378]

description: miRNA


title: Bladder Cancer [BL0398]

description: miRNA


title: Bladder Cancer [BL0388]

description: miRNA


title: Bladder Cancer [BL0368]

description: miRNA


title: Bladder Cancer [BL0383]

description: miRNA


title: Bladder Cancer [BL0373]

description: miRNA


title: Bladder Cancer [BL0365]

description: miRNA


title: Bladder Cancer [BL0366]

description: miRNA


title: Bladder Cancer [BL0364]

description: miRNA


title: Bladder Cancer [BL0381]

description: miRNA


title: Bladder Cancer [BL0359]

description: miRNA


title: Bladder Cancer [BL0360]

description: miRNA


title: Bladder Cancer [BL0361]

description: miRNA


title: Bladder Cancer [BL0362]

description: miRNA


title: Bladder Cancer [BL0353]

description: miRNA


title: Bladder Cancer [BL0351]

description: miRNA


title: Bladder Cancer [BL0355]

description: miRNA


title: Bladder Cancer [BL0356]

description: miRNA


title: Bladder Cancer [BL0348]

description: miRNA


title: Bladder Cancer [BL0349]

description: miRNA


title: Bladder Cancer [BL0350]

description: miRNA


title: Bladder Cancer [BL0346]

description: miRNA


title: Bladder Cancer [BL0343]

description: miRNA


title: Bladder Cancer [BL0329]

description: miRNA


title: Bladder Cancer [BL0344]

description: miRNA


title: Bladder Cancer [BL0328]

description: miRNA


title: Bladder Cancer [BL0325]

description: miRNA


title: Bladder Cancer [BL0323]

description: miRNA


title: Bladder Cancer [BL0327]

description: miRNA


title: Bladder Cancer [BL0340]

description: miRNA


title: Bladder Cancer [BL0335]

description: miRNA


title: Bladder Cancer [BL0333]

description: miRNA


title: Bladder Cancer [BL0339]

description: miRNA


title: Bladder Cancer [BL0331]

description: miRNA


title: Bladder Cancer [BL0330]

description: miRNA


title: Bladder Cancer [BL0332]

description: miRNA


title: Bladder Cancer [BL0326]

description: miRNA


title: Bladder Cancer [BL0337]

description: miRNA


title: Bladder Cancer [BL0320]

description: miRNA


title: Bladder Cancer [BL0322]

description: miRNA


title: Bladder Cancer [BL0319]

description: miRNA


title: Bladder Cancer [BL0318]

description: miRNA


title: Bladder Cancer [BL0317]

description: miRNA


title: Bladder Cancer [BL0315]

description: miRNA


title: Bladder Cancer [BL0336]

description: miRNA


title: Bladder Cancer [BL0324]

description: miRNA


title: Bladder Cancer [BL0310]

description: miRNA


title: Bladder Cancer [BL0311]

description: miRNA


title: Bladder Cancer [BL0308]

description: miRNA


title: Bladder Cancer [BL0307]

description: miRNA


title: Bladder Cancer [BL0304]

description: miRNA


title: Bladder Cancer [BL0294]

description: miRNA


title: Bladder Cancer [BL0296]

description: miRNA


title: Bladder Cancer [BL0290]

description: miRNA


title: Bladder Cancer [BL0300]

description: miRNA


title: Bladder Cancer [BL0299]

description: miRNA


title: Bladder Cancer [BL0292]

description: miRNA


title: Bladder Cancer [BL0280]

description: miRNA


title: Bladder Cancer [BL0283]

description: miRNA


title: Bladder Cancer [BL0301]

description: miRNA


title: Bladder Cancer [BL0293]

description: miRNA


title: Bladder Cancer [BL0291]

description: miRNA


title: Bladder Cancer [BL0313]

description: miRNA


title: Bladder Cancer [BL0314]

description: miRNA


title: Bladder Cancer [BL0282]

description: miRNA


title: Bladder Cancer [BL0298]

description: miRNA


title: Bladder Cancer [BL0287]

description: miRNA


title: Bladder Cancer [BL0288]

description: miRNA


title: Bladder Cancer [BL0279]

description: miRNA


title: Bladder Cancer [BL0286]

description: miRNA


title: Bladder Cancer [BL0309]

description: miRNA


title: Bladder Cancer [BL0285]

description: miRNA


title: Bladder Cancer [BL0276]

description: miRNA


title: Bladder Cancer [BL0278]

description: miRNA


title: Bladder Cancer [BL0275]

description: miRNA


title: Bladder Cancer [BL0273]

description: miRNA


title: Bladder Cancer [BL0271]

description: miRNA


title: Bladder Cancer [BL0274]

description: miRNA


title: Bladder Cancer [BL0270]

description: miRNA


title: Bladder Cancer [BL0269]

description: miRNA


title: Bladder Cancer [BL0267]

description: miRNA


title: Bladder Cancer [BL0266]

description: miRNA


title: Bladder Cancer [BL0257]

description: miRNA


title: Bladder Cancer [BL0264]

description: miRNA


title: Bladder Cancer [BL0256]

description: miRNA


title: Bladder Cancer [BL0263]

description: miRNA


title: Bladder Cancer [BL0250]

description: miRNA


title: Bladder Cancer [BL0265]

description: miRNA


title: Bladder Cancer [BL0249]

description: miRNA


title: Bladder Cancer [BL0251]

description: miRNA


title: Bladder Cancer [BL0259]

description: miRNA


title: Bladder Cancer [BL0258]

description: miRNA


title: Bladder Cancer [BL0246]

description: miRNA


title: Bladder Cancer [BL0253]

description: miRNA


title: Bladder Cancer [BL0260]

description: miRNA


title: Bladder Cancer [BL0255]

description: miRNA


title: Bladder Cancer [BL0262]

description: miRNA


title: Bladder Cancer [BL0252]

description: miRNA


title: Bladder Cancer [BL0243]

description: miRNA


title: Bladder Cancer [BL0244]

description: miRNA


title: Bladder Cancer [BL0245]

description: miRNA


title: Bladder Cancer [BL0238]

description: miRNA


title: Bladder Cancer [BL0240]

description: miRNA


title: Bladder Cancer [BL0237]

description: miRNA


title: Bladder Cancer [BL0261]

description: miRNA


title: Bladder Cancer [BL0236]

description: miRNA


title: Bladder Cancer [BL0234]

description: miRNA


title: Bladder Cancer [BL0248]

description: miRNA


title: Bladder Cancer [BL0241]

description: miRNA


title: UCMSC infected PRVABC59 48hpi

type: SRA

city: Seoul

first: Ok Sarah

last: Shin

address: 148, Gurodongro, Gurogu

country: South Korea

institute: Korea Univ.

library_source: transcriptomic

data_processing:
Sequencing Quality assessment refer to Wang L, Wang S, Li W., RSeQC: Quality Control of RNA-seq experiments. Bioinformatics. 2012 Jun 27 and Simon Andrews. FastQC: A quality control tool for high throughput sequence data.
Gene expression level was measured according to Wang L, Feng Z, Wang X, Wang X, Zhang X. Bioinformatics. 2010 Jan 1;26(1):136-8. Epub 2009 Oct 24. DEGseq: an R package for identifying differentially expressed genes from RNA-seq data.
Alignment was performed according to Li H, Durbin R. Bioinformatics. 2010 Mar 1;26(5):589-95. Epub 2010 Jan 15. Fast and accurate long-read alignment with Burrows-Wheeler transform.
Supplementary_files_format_and_content: excel files include GeneName, GeneDescription, Value_1, Value_2, log2foldchange, p_value, q_value.

source_name: Umbilical cord

growth_protocol: UC-MSC(Umbilical-cord derived mesenchymal stem cell) were infected with ZIKV strains for various time points on 6well (MOI=1) and RNA was harvested using TRI-Zol reagent.

extract_protocol:
Cell were lysed via Trizol; RNA isolated using RNA prep kit(Direct-zol™ RNA MiniPrep Plus)
TruSeq RNA Library Prep Kit

treatment_protocol: Plated UCMSC cells cultured in growth media containing virus for 1hour (for attachment); media changed to growth media(DMEM, 10% FBS, 1% NEAA, w/o Penicillin/Streptomycin)

tag: cell type

value: Mesenchymal Stem Cell

tag: passages

tag: strain

value: MSC


title: Bladder Cancer [BL0235]

description: miRNA


title: UCMSC infected MR766 4hpi


title: UCMSC infected MR766 48hpi


title: Bladder Cancer [BL0233]

description: miRNA


title: UCMSC Mock


title: Bladder Cancer [BL0220]

description: miRNA


title: Bladder Cancer [BL0232]

description: miRNA

value: Female


title: Bladder Cancer [BL0226]

description: miRNA


title: Bladder Cancer [BL0227]

description: miRNA


title: Bladder Cancer [BL0228]

description: miRNA


title: Bladder Cancer [BL0219]

description: miRNA


title: Bladder Cancer [BL0225]

description: miRNA


title: Bladder Cancer [BL0223]

description: miRNA


title: Bladder Cancer [BL0224]

description: miRNA


title: Bladder Cancer [BL0231]

description: miRNA


title: Bladder Cancer [BL0222]

description: miRNA


title: Bladder Cancer [BL0217]

description: miRNA


title: Bladder Cancer [BL0216]

description: miRNA


title: UCMSC infected PRVABC59 4hpi


title: Bladder Cancer [BL0211]

description: miRNA


title: Bladder Cancer [BL0212]

description: miRNA


title: Bladder Cancer [BL0210]

description: miRNA


title: Bladder Cancer [BL0206]

description: miRNA


title: Bladder Cancer [BL0205]

description: miRNA


title: RNA_NCI-H1650_GFPsg2_16

city: Boston

first: Tao

last: Zou

address: 450 Brookline Avenue

country: USA

institute: Dana-Farber Cancer Institute

data_processing:
STAR alignment
HTSeq quantification
DESeq2 normalization
GenePattern enrichment analysis
Genome_build: hg19
Supplementary_files_format_and_content: txt file for read counts output

source_name: Cancer cell line

growth_protocol: All cell lines were grown in RPMI 1640 media supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin

extract_protocol:
RNA was extracted using Qiagen RNeasy kit (74104) and mRNA was subsequently isolated after ribosomal RNA depletion using the NEBNext rRNA Depletion Kit (E6310)
The RNA library was constructed using the NEBNext Ultra Directional RNA Library Prep kit (E7420S)

treatment_protocol: The indicated samples were treated with vehicle or interferon-β at 10 ng/mL for 24 hours in RPMI 1640 media prior to collection for RNA-seq. The remaining samples were untreated.

tag: cell line

tag: tumor type

value: Lung adenocarcinoma

tag: genetic manipulation

value: NCI-H1650 expressing control sgRNA #2 against GFP

tag: treatment

value: Untreated


title: RNA_NCI-H196_GFPsg2_20

value: Variant small cell lung cancer

value: NCI-H196 expressing control sgRNA #2 against GFP


title: RNA_NCI-H1650_ADARsg1_13

value: NCI-H1650 expressing control sgRNA #1 against ADAR


title: RNA_NCI-H196_ADARsg2_19

value: NCI-H196 expressing control sgRNA #2 against ADAR


title: RNA_NCI-H196_ADARsg1_17

value: NCI-H196 expressing control sgRNA #1 against ADAR


title: RNA_NCI-H196_GFPsg1_18

value: NCI-H196 expressing control sgRNA #1 against GFP


title: RNA_NCI-H1650_GFPsg1_14

value: NCI-H1650 expressing control sgRNA #1 against GFP


title: Bladder Cancer [BL0203]

description: miRNA

value: Bladder Cancer

value: high


title: RNA_A549_ADARsg2_Vehicle_7

value: A549 expressing control sgRNA #2 against ADAR

value: Treated with vehicle for 24 hours


title: RNA_A549_ADARsg2_IFNB_11

value: Treated with interferon-Beta for 24 hours


title: RNA_A549_ADARsg1_IFNB_9

value: A549 expressing control sgRNA #1 against ADAR


title: RNA_A549_GFPsg2_IFNB_12

value: A549 expressing control sgRNA #2 against GFP


title: RNA_A549_ADARsg1_Vehicle_5

value: Lung adenocarcinoma


title: Bladder Cancer [BL0201]

description: miRNA

value: Serum

value: Male


title: RNA_NCI-H1650_ADARsg2_15

value: NCI-H1650 expressing control sgRNA #2 against ADAR

value: Untreated


title: Bladder Cancer [BL0200]

description: miRNA


title: RNA_HCC366_ADARsg2_3

value: Lung adenosquamous carcinoma

value: HCC366 expressing control sgRNA #2 against ADAR


title: Bladder Cancer [BL0197]

description: miRNA


title: Bladder Cancer [BL0199]

description: miRNA


title: Bladder Cancer [BL0198]

description: miRNA


title: RNA_HCC366_ADARsg1_1

value: HCC366 expressing control sgRNA #1 against ADAR


title: Bladder Cancer [BL0196]

description: miRNA


title: RNA_A549_GFPsg1_IFNB_10

value: A549 expressing control sgRNA #1 against GFP


title: Bladder Cancer [BL0195]

description: miRNA


title: RNA_HCC366_GFPsg1_2

value: HCC366 expressing control sgRNA #1 against GFP


title: RNA_A549_GFPsg1_Vehicle_6


title: RNA_HCC366_GFPsg2_4

value: HCC366 expressing control sgRNA #2 against GFP


title: Bladder Cancer [BL0194]

description: miRNA

value: Female

value: Bladder Cancer

value: low


title: Bladder Cancer [BL0190]

description: miRNA

value: high


title: Bladder Cancer [BL0186]

description: miRNA


title: Bladder Cancer [BL0188]

description: miRNA


title: C-4_16_d50

city: Los Angeles

first: Aaron

last: Gordon

state: CA

address: Gonda Building, 695 Charles E. Young Drive South

department: Neurology

institute: UCLA

data_processing:
Paired end reads were mapped using STAR17 to hg38 with Gencode v25 annotations.
Gene expression levels were quantified using rsem (v1.3.0)
Genome_build: hg38
Supplementary_files_format_and_content: csv file containing raw counts

source_name: human cortical spheroids

growth_protocol: hiPSCs were maintained on six-well plates coated with recombinant human vitronectin (1:100, Life Technologies) in Essential 8TM medium (Invitrogen). Cells were passaged every 4 days with 0.5 mM EDTA (pH 8.0, Life Technologies). For the generation of 3D spheroids, hiPSCs were incubated with Accutase (Innovate Cell Technologies) at 37oC for 7 minutes and dissociated into single cells. One day prior to spheroid formation, hiPSCs were exposed to 1% dimethyl sulfoxide (DMSO) in Essential 8TM medium. To obtain uniformly-sized spheroids, AggreWell-800TM (Stemcell Technologies) containing 300 microwells were used. Approximately 3 x 106 single cells were added per AggreWell-800TM well in Essential 8TM medium supplemented with the ROCK inhibitor Y-27632 (10 μM, EMD Chemicals), centrifuged to capture the cells in the microwells and incubated at 37oC with 5% CO2. After 24 hours, spheroids from each microwell were harvested by pipetting (with a cut P1000 tip) medium in the well up and down and transferred into ultra-low attachment plastic dishes (Corning) in Essential 6TM medium (Life Technologies) supplemented with two SMAD pathway inhibitors– dorsomorphin (2.5 μM, Sigma-Aldrich) and SB-431542 (10 μM, Tocris). For the first five days, Essential 6TM medium was changed every day and supplemented with dorsomorphin and SB-431542. The Wnt pathway inhibitor XAV-939 (2.5 μM, Tocris) was added with the two SMAD pathway inhibitors. On the sixth day in suspension, neural spheroids were transferred to neural medium containing Neurobasal A (Life Technologies, 10888), B-27 Supplement without vitamin A (#12587, Life Technologies) and GlutaMax (1:100, Life Technologies). The neural medium was supplemented with 20 ng/ml EGF (R&D Systems) and 20 ng/ml bFGF (R&D Systems) for 19 days (until day 24) with daily medium change in the first 10 days, and every other day for the subsequent 9 days. To promote differentiation of the neural progenitors into neurons, the neural medium was supplemented with 20 ng/ml BDNF (Peprotech) and 20 ng/ml NT3 (Peprotech) with medium changes every other day. From day 43, only neural medium without growth factors was used for medium changes every 4 days.

extract_protocol:
Following extraction of total RNA, rRNA was depleted (RiboZero Gold, Illumina) and libraries were prepared using Truseq stranded RNA RiboZero Gold (Illumina).
RNA libraries were prepared for sequencing using standard Illumina protocols

value: human cortical spheroids

value: F

tag: line

tag: culturing protocol

value: MEFs

tag: differentiation day

tag: race pc1

tag: race pc2

tag: differentiation


title: C-4_17_d100

tag: tissue

tag: Sex


title: C-5_16_d50

value: M


title: Bladder Cancer [BL0189]

description: miRNA

value: Serum

tag: age

tag: disease status

tag: pathological tstage


title: Bladder Cancer [BL0193]

description: miRNA

value: Male

tag: pathological grade


title: C-4_22_d75


title: C-1_16_d75


title: Bladder Cancer [BL0184]

description: miRNA


title: Bladder Cancer [BL0180]

description: miRNA


title: RNA_A549_GFPsg2_Vehicle_8

value: Lung adenocarcinoma

value: A549 expressing control sgRNA #2 against GFP

value: Treated with vehicle for 24 hours


title: C-2.1_17_d100


title: Bladder Cancer [BL0185]

description: miRNA


title: C-5_16_d25


title: C-2.1_16_d50


title: Bladder Cancer [BL0179]

description: miRNA


title: Bladder Cancer [BL0181]

description: miRNA


title: C-4_16_d75


title: C-2.1_18_d75


title: C-1_18_d75


title: Bladder Cancer [BL0178]

description: miRNA


title: Bladder Cancer [BL0177]

description: miRNA


title: H20961_7_d100


title: H20961_7_d75


title: H20961_7_d50


title: Bladder Cancer [BL0176]

description: miRNA


title: H20961_7_d25


title: C-4_10_d75


title: C-4_10_d50


title: C-2.2_10_d100


title: C-4_10_d25


title: C-2.2_10_d75


title: Bladder Cancer [BL0175]

description: miRNA


title: Bladder Cancer [BL0174]

description: miRNA


title: Bladder Cancer [BL0167]

description: miRNA


title: Bladder Cancer [BL0164]

description: miRNA


title: Bladder Cancer [BL0165]

description: miRNA


title: Bladder Cancer [BL0162]

description: miRNA


title: Bladder Cancer [BL0161]

description: miRNA


title: Bladder Cancer [BL0168]

description: miRNA


title: Bladder Cancer [BL0172]

description: miRNA


title: Bladder Cancer [BL0160]

description: miRNA


title: Bladder Cancer [BL0169]

description: miRNA


title: C-1_9_d100


title: C-1_9_d75


title: C-4_19_d100


title: C-1_9_d50


title: C-2.2_10_d25


title: C-1_9_d25


title: C-2.2_10_d50


title: C-3_13_d50


title: Bladder Cancer [BL0154]

description: miRNA


title: C-3_5_d75


title: Bladder Cancer [BL0155]

description: miRNA


title: Bladder Cancer [BL0152]

description: miRNA


title: Bladder Cancer [BL0153]

description: miRNA


title: Bladder Cancer [BL0158]

description: miRNA


title: H20961_1_d75


title: H20961_8_d100


title: H20961_8_d75


title: Bladder Cancer [BL0159]

description: miRNA


title: Bladder Cancer [BL0157]

description: miRNA


title: H20961_8_d50


title: Bladder Cancer [BL0150]

description: miRNA


title: Bladder Cancer [BL0151]

description: miRNA


title: H20961_8_d25


title: Bladder Cancer [BL0145]

description: miRNA


title: H20961_6_d75


title: H20961_6_d100


title: H20961_6_d50


title: Bladder Cancer [BL0146]

description: miRNA


title: H20961_6_d25


title: Bladder Cancer [BL0149]

description: miRNA


title: Bladder Cancer [BL0147]

description: miRNA


title: C-5_4_d75


title: Bladder Cancer [BL0143]

description: miRNA


title: Bladder Cancer [BL0142]

description: miRNA


title: Bladder Cancer [BL0144]

description: miRNA


title: Bladder Cancer [BL0141]

description: miRNA


title: H20961_1_d100


title: C-5_4_d50


title: Bladder Cancer [BL0140]

description: miRNA


title: C-5_3_d100


title: C-5_3_d75


title: Bladder Cancer [BL0139]

description: miRNA


title: C-5_4_d100


title: Bladder Cancer [BL0138]

description: miRNA


title: Bladder Cancer [BL0136]

description: miRNA


title: C-5_3_d25


title: Bladder Cancer [BL0134]

description: miRNA


title: C-5_3_d50


title: Bladder Cancer [BL0148]

description: miRNA


title: C-5_1_d100


title: Bladder Cancer [BL0132]

description: miRNA


title: C-5_1_d50


title: C-5_1_d25


title: C-4_3_d75


title: Bladder Cancer [BL0131]

description: miRNA


title: C-5_1_d75


title: C-4_3_d100


title: Bladder Cancer [BL0129]

description: miRNA


title: Bladder Cancer [BL0123]

description: miRNA


title: Bladder Cancer [BL0126]

description: miRNA


title: Bladder Cancer [BL0127]

description: miRNA


title: Bladder Cancer [BL0130]

description: miRNA


title: C-4_3_d50


title: C-5_4_d25


title: C-4_3_d25


title: C-4_1_d100


title: Bladder Cancer [BL0122]

description: miRNA


title: C-4_1_d50


title: Bladder Cancer [BL0121]

description: miRNA


title: Bladder Cancer [BL0116]

description: miRNA


title: Bladder Cancer [BL0118]

description: miRNA


title: Bladder Cancer [BL0120]

description: miRNA


title: C-3_3_d100


title: C-3_3_d75


title: C-4_1_d25


title: C-3_3_d25


title: C-3_3_d50


title: Bladder Cancer [BL0114]

description: miRNA


title: Bladder Cancer [BL0109]

description: miRNA


title: Bladder Cancer [BL0112]

description: miRNA


title: C-3_1_d50


title: C-3_1_d25


title: C-3_1_d75


title: Bladder Cancer [BL0111]

description: miRNA


title: Bladder Cancer [BL0106]

description: miRNA


title: C-2.2_1_d100


title: C-2.2_1_d50


title: C-2.2_1_d75


title: C-4_1_d75


title: Bladder Cancer [BL0102]

description: miRNA


title: Bladder Cancer [BL0098]

description: miRNA


title: Bladder Cancer [BL0097]

description: miRNA


title: Bladder Cancer [BL0094]

description: miRNA


title: C-2.2_1_d25


title: C-1_8_d100


title: Bladder Cancer [BL0093]

description: miRNA


title: C-1_8_d75


title: Bladder Cancer [BL0091]

description: miRNA


title: C-1_6_d100


title: C-1_8_d50


title: C-1_8_d25


title: Bladder Cancer [BL0090]

description: miRNA


title: Bladder Cancer [BL0107]

description: miRNA


title: Bladder Cancer [BL0105]

description: miRNA


title: C-1_6_d75


title: Bladder Cancer [BL0089]

description: miRNA


title: C-5_5_d50


title: Bladder Cancer [BL0088]

description: miRNA


title: Bladder Cancer [BL0100]

description: miRNA


title: Bladder Cancer [BL0087]

description: miRNA


title: C-3_13_d100


title: C-5_5_d25


title: C-2.2_12_d75


title: Bladder Cancer [BL0084]

description: miRNA


title: Bladder Cancer [BL0085]

description: miRNA


title: C-2.2_13_d25


title: Bladder Cancer [BL0078]

description: miRNA


title: Bladder Cancer [BL0092]

description: miRNA


title: C-1_20_d100


title: Bladder Cancer [BL0077]

description: miRNA


title: C-4_15_d75


title: C-4_11_d75


title: C-3_5_d100


title: C-5_14_d25


title: C-1_6_d50


title: C-2.2_13_d50


title: Bladder Cancer [BL0076]

description: miRNA


title: Bladder Cancer [BL0072]

description: miRNA


title: C-5_14_d50


title: Bladder Cancer [BL0075]

description: miRNA


title: C-2.2_12_d25


title: C-2.2_12_d100


title: Bladder Cancer [BL0074]

description: miRNA


title: Bladder Cancer [BL0071]

description: miRNA


title: Bladder Cancer [BL0083]

description: miRNA


title: Bladder Cancer [BL0080]

description: miRNA


title: Bladder Cancer [BL0082]

description: miRNA


title: Bladder Cancer [BL0066]

description: miRNA


title: Bladder Cancer [BL0068]

description: miRNA


title: Bladder Cancer [BL0067]

description: miRNA


title: C-1_14_d25


title: C-1_15_d75


title: Bladder Cancer [BL0061]

description: miRNA


title: Bladder Cancer [BL0060]

description: miRNA


title: Bladder Cancer [BL0058]

description: miRNA


title: H20961_2_d25


title: Bladder Cancer [BL0056]

description: miRNA


title: C-5_14_d75


title: Bladder Cancer [BL0070]

description: miRNA


title: C-4_11_d25


title: C-2.1_20_d50


title: C-1_14_d75


title: C-4_11_d100


title: C-5_14_d100


title: C-2.2_12_d50


title: C-4_11_d50


title: C-2.2_20_d50


title: Bladder Cancer [BL0055]

description: miRNA


title: Bladder Cancer [BL0053]

description: miRNA


title: C-3_5_d50


title: Bladder Cancer [BL0052]

description: miRNA


title: Bladder Cancer [BL0051]

description: miRNA


title: C-5_5_d75


title: Bladder Cancer [BL0048]

description: miRNA


title: Bladder Cancer [BL0047]

description: miRNA


title: Bladder Cancer [BL0050]

description: miRNA


title: C-1_21_d25


title: C-1_14_d50


title: C-2.1_15_d75


title: H20961_2_d50


title: Bladder Cancer [BL0042]

description: miRNA


title: Bladder Cancer [BL0046]

description: miRNA


title: C-2.2_13_d100


title: C-2.1_21_d25


title: Bladder Cancer [BL0041]

description: miRNA


title: Bladder Cancer [BL0040]

description: miRNA


title: C-1_14_d100


title: C-2.2_13_d75


title: HumanSpg-Seq9_C96

city: San Antonio

first: Brian

middle: P.

last: Hermann

state: TX

address: 1 UTSA Circle

department: Department of Biology

institute: University of Texas at San Antonio

data_processing:
Illumina base-calling (PE100) and demultiplexing with BCL-2-Fastq
Paired FASTQ files from each sample trimmed and the quality confirmed using FASTQC
Aligned to the human genome (hg19)  with TopHat (Galaxy v0.9)
Transcript abundance (FPKM) determined with Cufflinks (Galaxy v.2.2.1.0)
Quality control with the Picard tool Collectrnaseqmetrics was used to eliminate poorly performing cell samples
Genome_build: Human reference genome NCBI build 37, GRCh37 (hg19)
Supplementary_files_format_and_content: A single matrix file CSV containing columns of FPKM values for each cell. Rows are FPKM values for each gene in the reference annotation. First column is gene ID, first row is sample name.

molecule: polyA RNA

source_name: Adult testis

extract_protocol:
Suspensions of human seminiferous tubule cells were prepared from adult testicular tissue parenchyma by a two-step enzymatic digestion was as described previously (Hermann et al., 2007b; Hermann et al., 2009; Dovey et al., 2013). Briefly, testis tissue was digested with 2mg/ml collagenase type IV at 37oC with vigorous agitation, washed with HBSS to remove interstitial cells, followed by digestion with 0.25% trypsin/EDTA containing 1.4mg/ml DNase I for 15 minutes at 37oC with trituration every 5 minutes, and quenched with 10% FBS. In all cases, cell suspensions were strained (40um pore size for mouse, 70um pore size for humans) and suspended in MEMα containing 10% FBS. Testis cell suspensions were used for FACS to enrich spermatogonia essentially as described (Hermann et al., 2015). Briefly, cells were suspended (5-20 x 106 cells/ml) in ice-cold Dulbecco’s phosphate-buffered saline (DPBS) containing 10% FBS (DPBS+S), labeled with antibodies (see Key Resources Table), and subjected to FACS using  a FACS Aria (BD). Positive antibody labeling was determined by comparison to staining with isotype control antibodies. Human spermatogonia were isolated by sorting cells with the phenotype HLA-ABCnegative / CD49enegative / THY1dim / ITGA6+ / EpCAMdim dim (see Fig. 2C). These markers were chosen because previous results indicate they specifically label undifferentiated spermatogonia, including some with colonization potential based on xenotransplantation (Dovey et al., 2013; Altman et al., 2014; Smith et al., 2014; Valli et al., 2014). Human spermatogonia were used for single-cell RNA-seq facilitated by the Fluidigm C1 instrument essentially as described (Hermann et al., 2015; Wu et al., 2014). Briefly, single cells were captured on 10-17µm integrated fluidic circuit chips using the C1 Single-Cell Autoprep System (Fluidigm), stained with ethidium homodimer, and imaged on an AxioImager M1 (Zeiss), and used to prepare cDNA with SMARTer Ultra Low RNA Kit for the Fluidigm C1 System (v2 chemistry; Takara). Dead cells (ethidium+), multiplets and cells contaminated with debris were excluded from further analysis. Human spermatogonia were stratified post-hoc based on the levels of D4 mRNA to match EGFP epifluorescence intensity stratification used for cognate moues cells. ID4 mRNA levels were normalized on a scale from 0 to 1 (1 representing the greatest amount of ID4mRNA) and cells were ranked. ID4-high and ID4-low spermatogonia were defined as the upper (Q1) or lower (Q2) quartiles (25%) of ranked cells, respectively.
Amplified cDNA was quantified with PicoGreen fluorometry (ThermoFisher Scientific) on a Synergy II (Biotek) based on manufacturer recommendations and normalized cDNA mass from each cell was used for Nextera XT dual-index library preparation (Illumina) with modifications from manufacturer recommendations essentially as described (Mutoji et al., 2016). Single-cell libraries were pooled, qualified for fragment size and distribution on a 2100 Bioanalyzer (Agilent) and quantified.

treatment_protocol: De-identified, surgical excess normal adult human testicular tissue was obtained through the Infertility Center of St. Louis from patients undergoing a diagnostic testicular biopsy (n=28) in preparation for microscopic vasectomy reversal (MVR) or testicular sperm extraction (TESE) for obstructive azoospermia with the informed consent of patients (see Supplemental Table 2). Testicular tissue was also recovered from male organ donors (n=2) through the Texas Organ Sharing Alliance and the University of Texas Transplant Center with informed consent of the next of kin. The University of Texas at San Antonio IRB determined that research on these tissues did not constitute human subjects research (IRB #12-098N and #17-074N). The age of the individuals was recorded (median = 42yr, mean = 41.6 ± 1.3yr), and while organ donors tended to be younger (34.3 ± 7.2yr) than biopsy patients (42.4 ± 1.2yr), the difference was not significant (p=0.32). Procured tissue was transported to the laboratory on ice in either Lactated Ringer’s solution or minimal essential medium alpha (MEMα) containing 10% fetal bovine serum. The amount of time tissues were exposed to cold ischemia prior to processing was recorded (median = 18.7hr; mean = 24.7 ± 3.8 hr).

tag: id4 mrna quartile

tag: cell type

value: Spermatogonium


title: Bladder Cancer [BL0037]

description: miRNA


title: Bladder Cancer [BL0049]

description: miRNA


title: Bladder Cancer [BL0038]

description: miRNA


title: Bladder Cancer [BL0030]

description: miRNA


title: Bladder Cancer [BL0032]

description: miRNA


title: Bladder Cancer [BL0039]

description: miRNA


title: Bladder Cancer [BL0033]

description: miRNA


title: Bladder Cancer [BL0031]

description: miRNA


title: HumanSpg-Seq9_C95


title: HumanSpg-Seq9_C93


title: HumanSpg-Seq9_C92


title: HumanSpg-Seq9_C91


title: HumanSpg-Seq9_C89


title: Bladder Cancer [BL0023]

description: miRNA


title: HumanSpg-Seq9_C87


title: Bladder Cancer [BL0024]

description: miRNA


title: Bladder Cancer [BL0029]

description: miRNA


title: Bladder Cancer [BL0020]

description: miRNA


title: C-3_13_d75


title: HumanSpg-Seq9_C86


title: Bladder Cancer [BL0019]

description: miRNA


title: Bladder Cancer [BL0018]

description: miRNA


title: HumanSpg-Seq9_C85


title: Bladder Cancer [BL0028]

description: miRNA


title: HumanSpg-Seq9_C81


title: Bladder Cancer [BL0014]

description: miRNA


title: HumanSpg-Seq9_C80


title: HumanSpg-Seq9_C74


title: HumanSpg-Seq9_C78


title: HumanSpg-Seq9_C76


title: HumanSpg-Seq9_C73


title: HumanSpg-Seq9_C75


title: HumanSpg-Seq9_C71


title: HumanSpg-Seq9_C72


title: Bladder Cancer [BL0022]

description: miRNA


title: Bladder Cancer [BL0008]

description: miRNA


title: HumanSpg-Seq9_C70


title: Bladder Cancer [BL0013]

description: miRNA


title: Bladder Cancer [BL0009]

description: miRNA


title: HumanSpg-Seq9_C69


title: Bladder Cancer [BL0004]

description: miRNA


title: Bladder Cancer [BL0011]

description: miRNA


title: Bladder Cancer [BL0012]

description: miRNA


title: Bladder Cancer [BL0003]

description: miRNA


title: Bladder Cancer [BL0017]

description: miRNA


title: HumanSpg-Seq9_C82


title: HeLa WT poly(A)+ RNA-Seq

city: Shanghai

first: Li

last: Yang

address: 131 Dong-An Road

department: Institutes of Biological Sciences

country: China

institute: Fudan University

data_processing:
High-throughput sequencing reads were trimmed using Trimmomatic (v.0.36) to remove adapters and low quality reads
Trimmed reads were aligned against GRCh38/hg38 using HISAT2 (v2.0.5), two mismatches were allowed for each read.
Mapped reads in SAM format for each sample were transferred into bigWig format using utilities supported by UCSC for data visualization.
Genome_build: GRCh38/hg38
Supplementary_files_format_and_content: bigWig

source_name: HeLa cells

growth_protocol: HeLa cells were maintained in DMEM supplemented with 10% FBS and penicillin/streptomycin.

extract_protocol:
For RNA, Cells were homogenized with dounce and pellet the nucleus suspended in WB100 buffer (10 mM HEPES pH7.5, 100 mM NaCl, 1 mM PMSF, 0.1% N-lauroylsarcosine, 40 U/mL RNasin) followed by sonication with S220 Focused-ultrasonicators (Covaris) (100 PW, 20% duty cycle, 200 burst, 1.5 min, 4℃). The lysate was digested with DNase at 37℃ for 15 min and stoped by adding 10 mM EDTA and 5 mM EGTA. Then, the lysate was pre-cleared with 50 μL Strepavidin C-1 magnetic beads with at 4℃ for 20 min. Then the pre-cleared lysates were hybridized with 350 nM probes at room temperature for 6 hours followed by adding 150 μL Strepavidin C-1 magnetic beads and incubating overnight at room temperature. The beads were washed with WB250 buffer (10 mM HEPES pH7.5, 250 mM NaCl, 0.2% SDS, 1 mM EGTA, 2mM EDTA, 1 mM PMSF, 0.1% N-lauroylsarcosine,) four times for 5 min each, and then were digested by Proteinase K at 50℃ for 1 hour followed by boiling at 100℃ for 10 min. Finally, 500 µL Trizol was added for RNA isolation.
RNA-seq libraries were prepared with Illumina TruSeq Stranded mRNA Sample Preparation Kit and CHART-seq libraries were prepared with Illumina TruSeq RNA Sample Preparation v2. Deep sequencing was performed on Illumina Hiseq X ten and Hiseq 2000 at CAS-MPG Partner Institute for Computational Biology Omics Core, Shanghai, China.

treatment_protocol: Plasmid transfection was carried out with X-tremeGENE 9 (Roche) for HeLa (60%–70% confluence) according to the manufacturer’s protocol, with 80%~90% transfection efficiency in general.

tag: cell line

value: HeLa

tag: genotype/variation

value: Wild Type


title: HeLa NEAT1 KO2 poly(A)+ RNA-Seq

value: NEAT1 KO


title: HumanSpg-Seq9_C68


title: HumanSpg-Seq9_C77


title: HumanSpg-Seq9_C67


title: Bladder Cancer [BL0007]

description: miRNA


title: HeLa NEAT1 KO1 poly(A)+ RNA-Seq


title: Bladder Cancer [BL0001]

description: miRNA


title: HumanSpg-Seq9_C64


title: HumanSpg-Seq9_C66


title: HumanSpg-Seq9_C63


title: HumanSpg-Seq9_C62


title: HumanSpg-Seq9_C61


title: HumanSpg-Seq9_C60


title: HumanSpg-Seq9_C59


title: wt2

city: Freising

first: Tobias

last: Fromme

address: Gregor-Mendel-Str. 2

department: Molecular Nutritional Medicine

country: Germany

institute: Technical University of Munich

data_processing:
Sequencing software: HiSeq Control Software 2.2.58 Image analysis and base calling: Real-Time Analysis (RTA) 1.18.64 fastq-file generation: CASAVA BCL2FASTQ Conversion Software 1.8.3
Sequenced reads with masked adaptor sequence were mapped to the murine genome, NCBI build 38, by the Genomatix Mapper 3.7.6 (Genomatix Mining Station, settings: 'deep', min. quality 92%).
Unique hits were subjected to expression analysis for RNA-seq (Genomatix Genome Analyzer, ElDorado version 12-2013) and provided as reads per kilobase of exon per megabase of library size (RPKM).
Genome_build: murine NCBI genome build 38
Supplementary_files_format_and_content: tab-delimited text file providing RPKM values for each sample.

organism: Mus musculus

source_name: interscapular brown adipose tissue

extract_protocol:
Interscapular brown adipose tissue was prepared and snap frozen in liquid nitrogen. Total RNA was prepared from Trisure (Bioline) and column-purified (SV Total RNA Isolation System, Promega).
Libraries were constructed from total RNA (TruSeq RNA Sample Prep kit v2 (Illumina)).

tag: genotype

value: wildtype

tag: strain


title: wt3


title: HumanSpg-Seq9_C58


title: wt1


title: ko3


title: ko1


title: ko2


title: HumanSpg-Seq9_C51


title: HumanSpg-Seq9_C52


title: HumanSpg-Seq9_C54


title: HumanSpg-Seq9_C53


title: HumanSpg-Seq9_C55


title: HumanSpg-Seq9_C57


title: HumanSpg-Seq9_C56


title: HumanSpg-Seq9_C50


title: HumanSpg-Seq9_C49


title: HumanSpg-Seq9_C43


title: E2_Rep3

city: Kansas City

first: Stefan

middle: Hannes

last: Graw

address: 3901 Rainbow Blvd

department: Biostatistics

institute: University of Kansas Medical Center

description: G1E-ER4 cells carry a GATA-1 transcription factor fused to the estrogen receptor (GATA-1ER) that undergo erythropoiesis following the addition of β-estradiol (E2)

data_processing:
Alignment and calculate expression values using RSEM with the following parameters
rsem-calculate-expression --bowtie2 --output-genome-bam --paired-end
Differential expression analysis of RNA-seq expression profiles using EdgeR
Genome_build: GRCm38 (mm10)
Supplementary_files_format_and_content: Tab delimited gene expression file including gene_id, transcript_id(s), length, effective_length, expected_count, TPM, FPKM

growth_protocol: G1E-ER4 cells were cultured in IMDM (12440061, ThermoFisher Scientific) supplemented with 15% heat-inactivated FBS (Gemini), 100 U/ml penicillin and 100 μg/ml streptomycin (P4333, Sigma), 120 nM 1-thioglycerol (M6145, Sigma), 2.5 U/ml recombinant mouse erythropoietin (rm EPO, 587606, Biolegend), and 50 ng/ml recombinant mouse stem cell factor (rm SCF, 78064.2, Stemcell Technology). Puromycin was added to the G1E-ER4 medium at 1 μg/ml to maintain selection pressure. Cells were incubated at 37°C, 5% CO2 in a 95% humidified incubator.

extract_protocol:
Total RNA was isolated from 5,000,000 cells using TRI Reagent (T9424, Sigma) according to the manufacturer’s instructions.
Library was prepared using Illumina TruSeq Stranded mRNA sample preparation kit (Illumina) as manufacturer’s instruction. 800 ng of the total RNA was used for each library preparation.

treatment_protocol: Cells were seeded at 40,000 cells/ml in T25 flasks with 10 ml culture medium overnight. Cells were then treated with 10 μM Thiamet-G (TMG, S.D. Specialty Chemicals) or/and 1 μM β-estradiol (E2, E2758, Sigma) for 30 hrs.

tag: treatment

value: E2 for 30h

tag: passages

value: Embryonic stem cell

tag: developmental stage

value: Blastocyst stage


title: TMG_E2_Rep2

value: TMG and E2 for 30h


title: TMG_Rep2

value: TMG for 30h


title: TMG_E2_Rep3


title: HumanSpg-Seq9_C41


title: TMG_E2_Rep1


title: SKOV-3 LV-HCG-3

first: Xiaoqing

last: Guo

address: 2699 west gaoke road

department: Obstetrics and Gynecology

institute: Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine

description: Biological replicate 3 of 3. Stable β-HCG-gene transfected ovarian cancer SKOV3 cells, harvested after several passages.

hyb_protocol: Each slide was hybridized with 1.65μg Cy3-labeled cRNA using Gene Expression Hybridization Kit (Cat.# 5188-5242, Agilent technologies, Santa Clara, CA, US) in Hybridization Oven (Cat.# G2545A, Agilent technologies, Santa Clara, CA, US), according to the manufacturer’s instructions. After 17 hours hybridization, slides were washed in staining dishes (Cat.# 121, Thermo Shandon, Waltham, MA, US) with Gene Expression Wash Buffer Kit(Cat.# 5188-5327, Agilent technologies, Santa Clara, CA, US), followed the manufacturer’s instructions。

scan_protocol: Slides were scanned by Agilent Microarray Scanner (Cat#G2565CA, Agilent technologies, Santa Clara, CA, US) with default settings, Dye channel: Green, Scan resolution=3μm, PMT 100%, 20bit.

data_processing: Data were extracted with Feature Extraction software 10.7 (Agilent technologies, Santa Clara, CA, US). Raw data were normalized by Quantile algorithm, GeneSpring Software 12.6.1 (Agilent technologies, Santa Clara, CA, US).

source_name: SKOV3 cells

label_protocol: Total RNA was amplified and labeled by Low Input Quick Amp WT Labeling Kit (Cat.# 5190-2943, Agilent technologies, Santa Clara, CA, US), following the manufacturer’s instructions. Labeled cRNA were purified by RNeasy mini kit (Cat.# 74106, QIAGEN, GmBH, Germany).

growth_protocol: SKOV3 cells were cultured in RPMI-1640 medium (HyClone, USA), containing 10% fetal bovine serum (FBS, Gibco, USA), 100 units/mL penicillin, and 100 mg/mL streptomycin, at 37°C in a humidified 5% CO2 incubator.

extract_protocol: Total RNA was extracted and purified using miRNeasy Mini Kit（Cat#217004，QIAGEN, GmBH, Germany) following the manufacturer’s instructions and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US).

treatment_protocol: For overexpression of β-HCG, SKOV3 cells were transfected with lentiviral vector with eGFP, encoding β-HCG and negative control vector (LV- β-HCG and LV-vector) by using polybrene (5.0 µg/mL). Cells were selected with medium containing 0.2 mg/mL puromycin after 48h of infection. The dysregulation of β-HCG was confirmed by using RT-PCR.

value: ovarian cancer cells


title: SKOV-3 LV-CON-2

description: Biological replicate 2 of 3. Control negative vector transfected ovarian cancer SKOV3 cells,untreated, harvested after several passages.


title: E2_Rep1


title: E2_Rep2


title: TMG_Rep1


title: SKOV-3 LV-CON-1

description: Biological replicate 1 of 3. Control negative vector transfected ovarian cancer SKOV3 cells,untreated, harvested after several passages.


title: Ctrl_Rep1


title: SKOV-3 LV-CON-3

description: Biological replicate 3 of 3. Control negative vector transfected ovarian cancer SKOV3 cells,untreated, harvested after several passages.


title: SKOV-3 LV-HCG-2

description: Biological replicate 2 of 3. Stable β-HCG-gene transfected ovarian cancer SKOV3 cells, harvested after several passages.


title: HumanSpg-Seq9_C36


title: HumanSpg-Seq9_C37


title: HumanSpg-Seq9_C35


title: RNA-Seq of SSKO-2

city: Irvine

first: Daniel

last: Haensel

state: California

address: Medical Sciences D288

institute: UC Irvine

description:
Ovol2_MutantvsControl_fpkm.txt
Ovol2_MutantvsControl_Processed.txt

data_processing:
Raw reads processed and trimmed using quality threshold of 15 and Truseq adapter sequences
Trimmed reads shorter than 20bp were discarded
Refrence genome sequence mm10 and its transcriptome annotation was collected and indexed for maping using tophat iGenome website version
Trimmed paired end reads were mapped to refrence sequences with gene annotation
Gene expression level quantified using HTSeq
Genome_build: mm10
Supplementary_files_format_and_content: FastQ

source_name: Newborn keratinocytes

growth_protocol: Keratinocytes were isolated from newborn (P0) mice and cultured on mitotically inactivated J2-3T3 using low calcium E-media

extract_protocol:
Isolated before conlfuent to avoid spontanous differentiation, feeders completely removed, RNA isolated using Trizol reagent
Full-length cDNA library amplification, tagmentation of cDNA via Nextera DNA sample preparation kit

treatment_protocol: No additional treatment

value: keratinocyte

value: newborn

tag: passage


title: RNA-Seq of Control-2

value: control


title: HumanSpg-Seq9_C34


title: Ctrl_Rep2


title: HumanSpg-Seq9_C39


title: HumanSpg-Seq9_C40


title: RNA-Seq of SSKO-1


title: Ctrl_Rep3


title: TMG_Rep3


title: HumanSpg-Seq9_C32


title: HumanSpg-Seq9_C30


title: Ad-Id4GFP-Seq4_C96

data_processing:
Illumina base-calling (PE100) and demultiplexing with BCL-2-Fastq
Paired FASTQ files from each sample trimmed and the quality confirmed using FASTQC
Aligned to the mouse genome (mm10)  with TopHat (Galaxy v0.9)
Transcript abundance (FPKM) determined with Cufflinks (Galaxy v.2.2.1.0)
Quality control with the Picard tool Collectrnaseqmetrics was used to eliminate poorly performing cell samples
Genome_build: Mouse reference genome NCBI build 38, GRCm38 (mm10)
Supplementary_files_format_and_content: A single matrix file CSV containing columns of FPKM values for each cell. Rows are FPKM values for each gene in the reference annotation. First column is gene ID, first row is sample name.

growth_protocol: Normal mouse husbandry with ad libitum food and water.

extract_protocol:
Single-cell suspensions of seminiferous tubules were generated from adult mouse testes using a two-step enzymatic digestion approach. Briefly, testicular parenchyma from 2 or more adult mice were digested with 1mg/ml Collagenase Type IV (Worthington Biochemicals) for 2-3 minutes at 37oC, washed with Hank’s Buffered Salt Solution (HBSS) to remove interstitial cells, digested with 0.25% trypsin/EDTA containing 1.4mg/ml DNase I (Sigma) for 7-9 minutes at 37oC, and quenched with 10% FBS. Testis cell suspensions were used for FACS to enrich spermatogonia essentially as described (Hermann et al., 2015). Briefly, cells were suspended (5-20 x 10^6 cells/ml) in ice-cold Dulbecco’s phosphate-buffered saline (DPBS) containing 10% FBS (DPBS+S). Cells were pre-enriched for spermatogonia by density centrifugation in DPBS+S over a 30% Percoll cushion (Sigma) for 8 minutes at 600xg without braking. Pelleted cells were subjected to FACS and spermatogonia isolated based on gating CD9-bright and ID4-EGFP double-positive cells using  a FACS Aria (BD). CD9bright/ID4-EGFP+ spermatogonia adult mice were used for single-cell RNA-seq facilitated by the Fluidigm C1 instrument essentially as described (Hermann et al., 2015; Wu et al., 2014). Briefly, single cells were captured on 10-17µm integrated fluidic circuit chips using the C1 Single-Cell Autoprep System (Fluidigm), stained with ethidium homodimer, and imaged on an AxioImager M1 (Zeiss), and used to prepare cDNA with SMARTer Ultra Low RNA Kit for the Fluidigm C1 System (v2 chemistry; Takara). Dead cells (ethidium+), multiplets and cells contaminated with debris were excluded from further analysis. Mouse ID4-EGFP+ spermatogonia were stratified based on the EGFP epifluorescence intensity using the interactive measurement module of AxioVision 4.8.2 (Zeiss) and images taken of each cell. The densitometric mean value of the EGFP channel for each cell was normalized on a scale from 0 to 1 (1 representing the brightest EGFP+ cell) and cells were ranked. ID4-EGFPbright and ID4-EGFPdim spermatogonia were defined as the upper (Q1) or lower (Q2) quartiles (25%) of ranked EGFP+ cells, respectively.
Amplified cDNA was quantified with PicoGreen fluorometry (ThermoFisher Scientific) on a Synergy II (Biotek) based on manufacturer recommendations and normalized cDNA mass from each cell was used for Nextera XT dual-index library preparation (Illumina) with modifications from manufacturer recommendations essentially as described (Mutoji et al., 2016). Single-cell libraries were pooled, qualified for fragment size and distribution on a 2100 Bioanalyzer (Agilent) and quantified.

treatment_protocol: Animal were untreated.

tag: egfp epifluorescence quartile


title: SKOV-3 LV-HCG-1

description: Biological replicate 1 of 3. Stable β-HCG-gene transfected ovarian cancer SKOV3 cells, harvested after several passages.

tag: tissue


title: HumanSpg-Seq9_C31


title: RNA-Seq of Control-1


title: Ad-Id4GFP-Seq4_C94


title: Ad-Id4GFP-Seq4_C93


title: HumanSpg-Seq9_C29


title: HumanSpg-Seq9_C28


title: Ad-Id4GFP-Seq4_C92


title: HumanSpg-Seq9_C27


title: HumanSpg-Seq9_C26


title: Ad-Id4GFP-Seq4_C89


title: HumanSpg-Seq9_C25


title: Ad-Id4GFP-Seq4_C88


title: HumanSpg-Seq9_C23


title: Ad-Id4GFP-Seq4_C87


title: Ad-Id4GFP-Seq4_C86


title: HumanSpg-Seq9_C21


title: HumanSpg-Seq9_C22


title: HumanSpg-Seq9_C19


title: HumanSpg-Seq9_C20


title: Ad-Id4GFP-Seq4_C81


title: Ad-Id4GFP-Seq4_C85


title: Ad-Id4GFP-Seq4_C80


title: HumanSpg-Seq9_C18


title: Ad-Id4GFP-Seq4_C79


title: HumanSpg-Seq9_C16


title: Ad-Id4GFP-Seq4_C82


title: Ad-Id4GFP-Seq4_C83


title: HumanSpg-Seq9_C13


title: Ad-Id4GFP-Seq4_C77


title: Ad-Id4GFP-Seq4_C75


title: Ad-Id4GFP-Seq4_C76


title: HumanSpg-Seq9_C09


title: Ad-Id4GFP-Seq4_C74


title: HumanSpg-Seq9_C08


title: Ad-Id4GFP-Seq4_C70


title: Ad-Id4GFP-Seq4_C73


title: HumanSpg-Seq9_C06


title: Ad-Id4GFP-Seq4_C68


title: HumanSpg-Seq9_C17


title: HumanSpg-Seq9_C05


title: HumanSpg-Seq9_C04


title: Ad-Id4GFP-Seq4_C66


title: Ad-Id4GFP-Seq4_C67


title: HumanSpg-Seq9_C02


title: Ad-Id4GFP-Seq4_C62


title: Ad-Id4GFP-Seq4_C64


title: HumanSpg-Seq7_C96


title: HumanSpg-Seq9_C01


title: Ad-Id4GFP-Seq4_C60


title: HumanSpg-Seq9_C11


title: Ad-Id4GFP-Seq4_C65


title: Ad-Id4GFP-Seq4_C59


title: HumanSpg-Seq7_C94


title: HumanSpg-Seq7_C93


title: Ad-Id4GFP-Seq4_C61


title: HumanSpg-Seq9_C10


title: HumanSpg-Seq7_C92


title: HumanSpg-Seq7_C95


title: Ad-Id4GFP-Seq4_C57


title: Ad-Id4GFP-Seq4_C58


title: Ad-Id4GFP-Seq4_C56


title: HumanSpg-Seq7_C90


title: HumanSpg-Seq7_C91


title: Ad-Id4GFP-Seq4_C54


title: HumanSpg-Seq7_C88


title: HumanSpg-Seq9_C03


title: Ad-Id4GFP-Seq4_C55


title: HumanSpg-Seq9_C83


title: Ad-Id4GFP-Seq4_C52


title: Ad-Id4GFP-Seq4_C53


title: HumanSpg-Seq7_C86


title: HumanSpg-Seq7_C85


title: Ad-Id4GFP-Seq4_C50


title: Ad-Id4GFP-Seq4_C48


title: Ad-Id4GFP-Seq4_C51


title: HumanSpg-Seq7_C84


title: HumanSpg-Seq7_C83


title: Ad-Id4GFP-Seq4_C47


title: HumanSpg-Seq7_C82


title: Ad-Id4GFP-Seq4_C46


title: Ad-Id4GFP-Seq4_C45


title: HumanSpg-Seq7_C80


title: Ad-Id4GFP-Seq4_C44


title: HumanSpg-Seq7_C78


title: HumanSpg-Seq7_C77


title: HumanSpg-Seq7_C76


title: HumanSpg-Seq7_C74


title: HumanSpg-Seq7_C87


title: Ad-Id4GFP-Seq4_C42


title: Ad-Id4GFP-Seq4_C41


title: Ad-Id4GFP-Seq4_C38


title: Ad-Id4GFP-Seq4_C43


title: HumanSpg-Seq7_C73


title: HumanSpg-Seq7_C72


title: Ad-Id4GFP-Seq4_C40


title: Ad-Id4GFP-Seq4_C36


title: HumanSpg-Seq7_C70


title: HumanSpg-Seq7_C71


title: Ad-Id4GFP-Seq4_C35


title: Ad-Id4GFP-Seq4_C37


title: HumanSpg-Seq7_C68


title: HumanSpg-Seq7_C67


title: HumanSpg-Seq7_C66


title: HumanSpg-Seq7_C79


title: Ad-Id4GFP-Seq4_C31


title: HumanSpg-Seq7_C64


title: HumanSpg-Seq7_C89


title: Ad-Id4GFP-Seq4_C33


title: HumanSpg-Seq7_C63


title: Ad-Id4GFP-Seq4_C28


title: HumanSpg-Seq7_C62


title: HumanSpg-Seq7_C61


title: Ad-Id4GFP-Seq4_C26


title: HumanSpg-Seq7_C60


title: HumanSpg-Seq7_C59


title: Ad-Id4GFP-Seq4_C25


title: Ad-Id4GFP-Seq4_C23


title: HumanSpg-Seq7_C57


title: Ad-Id4GFP-Seq4_C34


title: HumanSpg-Seq7_C58


title: Ad-Id4GFP-Seq4_C21


title: HumanSpg-Seq7_C55


title: Ad-Id4GFP-Seq4_C22


title: Ad-Id4GFP-Seq4_C19


title: Ad-Id4GFP-Seq4_C30


title: Ad-Id4GFP-Seq4_C18


title: HumanSpg-Seq7_C54


title: HumanSpg-Seq7_C53


title: Ad-Id4GFP-Seq4_C29


title: HumanSpg-Seq7_C52


title: HumanSpg-Seq7_C56


title: HumanSpg-Seq7_C28


title: Ad-Id4GFP-Seq4_C17


title: Ad-Id4GFP-Seq4_C14


title: HumanSpg-Seq7_C51


title: Ad-Id4GFP-Seq4_C16


title: HumanSpg-Seq7_C19


title: HumanSpg-Seq7_C24


title: Ad-Id4GFP-Seq4_C24


title: Ad-Id4GFP-Seq4_C13


title: HumanSpg-Seq7_C18


title: Ad-Id4GFP-Seq4_C11


title: HumanSpg-Seq7_C17


title: Ad-Id4GFP-Seq4_C10


title: Ad-Id4GFP-Seq4_C08


title: Ad-Id4GFP-Seq4_C20


title: HumanSpg-Seq7_C14


title: Ad-Id4GFP-Seq4_C07


title: Ad-Id4GFP-Seq4_C06


title: Ad-Id4GFP-Seq4_C04


title: HumanSpg-Seq7_C11


title: HumanSpg-Seq7_C12


title: Ad-Id4GFP-Seq4_C15


title: HumanSpg-Seq7_C10


title: HumanSpg-Seq7_C08


title: Ad-Id4GFP-Seq4_C02


title: HumanSpg-Seq7_C09


title: Ad-Id4GFP-Seq4_C01


title: HumanSpg-Seq7_C07


title: Ad-Id4GFP-Seq3_C96


title: HumanSpg-Seq7_C05


title: HumanSpg-Seq7_C03


title: Ad-Id4GFP-Seq3_C93


title: HumanSpg-Seq6_C95


title: Ad-Id4GFP-Seq4_C12


title: Ad-Id4GFP-Seq3_C92


title: Ad-Id4GFP-Seq3_C91


title: HumanSpg-Seq7_C16


title: HumanSpg-Seq6_C94


title: Ad-Id4GFP-Seq3_C90


title: HumanSpg-Seq7_C13


title: Ad-Id4GFP-Seq3_C87


title: Ad-Id4GFP-Seq3_C86


title: Ad-Id4GFP-Seq3_C84


title: HumanSpg-Seq6_C92


title: Ad-Id4GFP-Seq3_C80


title: Ad-Id4GFP-Seq3_C83


title: HumanSpg-Seq6_C86


title: HumanSpg-Seq6_C89


title: Ad-Id4GFP-Seq3_C81


title: HumanSpg-Seq6_C88


title: HumanSpg-Seq6_C90


title: Ad-Id4GFP-Seq3_C79


title: HumanSpg-Seq6_C83


title: Ad-Id4GFP-Seq3_C95


title: HumanSpg-Seq6_C81


title: HumanSpg-Seq6_C82


title: Ad-Id4GFP-Seq3_C76


title: HumanSpg-Seq6_C80


title: Ad-Id4GFP-Seq3_C75


title: Ad-Id4GFP-Seq3_C78


title: Ad-Id4GFP-Seq3_C74


title: HumanSpg-Seq6_C79


title: HumanSpg-Seq6_C78


title: Ad-Id4GFP-Seq3_C70


title: Ad-Id4GFP-Seq3_C69


title: HumanSpg-Seq6_C75


title: HumanSpg-Seq6_C73


title: HumanSpg-Seq6_C93


title: Ad-Id4GFP-Seq3_C66


title: Ad-Id4GFP-Seq3_C67


title: Ad-Id4GFP-Seq3_C65


title: HumanSpg-Seq6_C72


title: HumanSpg-Seq6_C70


title: Ad-Id4GFP-Seq3_C64


title: Ad-Id4GFP-Seq3_C63


title: HumanSpg-Seq6_C68


title: HumanSpg-Seq6_C69


title: Ad-Id4GFP-Seq3_C61


title: Ad-Id4GFP-Seq3_C62


title: HumanSpg-Seq6_C67


title: HumanSpg-Seq6_C77


title: Ad-Id4GFP-Seq3_C59


title: HumanSpg-Seq6_C63


title: HumanSpg-Seq6_C64


title: Ad-Id4GFP-Seq3_C71


title: HumanSpg-Seq6_C66


title: HumanSpg-Seq6_C71


title: Ad-Id4GFP-Seq3_C55


title: Ad-Id4GFP-Seq3_C60


title: Ad-Id4GFP-Seq3_C58


title: Ad-Id4GFP-Seq3_C53


title: HumanSpg-Seq6_C65


title: HumanSpg-Seq6_C62


title: Ad-Id4GFP-Seq3_C57


title: HumanSpg-Seq6_C60


title: HumanSpg-Seq6_C61


title: Ad-Id4GFP-Seq3_C52


title: HumanSpg-Seq6_C59


title: HumanSpg-Seq6_C57


title: Ad-Id4GFP-Seq3_C48


title: Ad-Id4GFP-Seq3_C43


title: Ad-Id4GFP-Seq3_C47


title: HumanSpg-Seq6_C55


title: HumanSpg-Seq6_C56


title: HumanSpg-Seq6_C54


title: Ad-Id4GFP-Seq3_C40


title: HumanSpg-Seq6_C50


title: Ad-Id4GFP-Seq3_C39


title: Ad-Id4GFP-Seq3_C36


title: Ad-Id4GFP-Seq3_C34


title: HumanSpg-Seq6_C49


title: HumanSpg-Seq6_C45


title: Ad-Id4GFP-Seq3_C50


title: Ad-Id4GFP-Seq3_C30


title: Ad-Id4GFP-Seq3_C31


title: HumanSpg-Seq6_C42


title: HumanSpg-Seq6_C43


title: Ad-Id4GFP-Seq3_C32


title: HumanSpg-Seq6_C44


title: HumanSpg-Seq6_C41


title: HumanSpg-Seq6_C38


title: Ad-Id4GFP-Seq3_C27


title: HumanSpg-Seq6_C40


title: HumanSpg-Seq6_C37


title: HumanSpg-Seq6_C36


title: HumanSpg-Seq6_C35


title: Ad-Id4GFP-Seq3_C24


title: Ad-Id4GFP-Seq3_C26


title: Ad-Id4GFP-Seq3_C23


title: Ad-Id4GFP-Seq3_C41


title: Ad-Id4GFP-Seq3_C25


title: Ad-Id4GFP-Seq3_C37


title: HumanSpg-Seq6_C34


title: HumanSpg-Seq6_C52


title: Ad-Id4GFP-Seq3_C20


title: Ad-Id4GFP-Seq3_C29


title: Ad-Id4GFP-Seq3_C21


title: HumanSpg-Seq6_C53


title: HumanSpg-Seq6_C33


title: HumanSpg-Seq6_C30


title: Ad-Id4GFP-Seq3_C17


title: HumanSpg-Seq6_C29


title: Ad-Id4GFP-Seq3_C18


title: HumanSpg-Seq6_C28


title: HumanSpg-Seq6_C32


title: Ad-Id4GFP-Seq3_C16


title: Ad-Id4GFP-Seq3_C19


title: HumanSpg-Seq6_C27


title: Ad-Id4GFP-Seq3_C15


title: Ad-Id4GFP-Seq3_C13


title: HumanSpg-Seq6_C25


title: Ad-Id4GFP-Seq3_C14


title: HumanSpg-Seq6_C26


title: HumanSpg-Seq6_C23


title: HumanSpg-Seq6_C19


title: Ad-Id4GFP-Seq3_C10


title: HumanSpg-Seq6_C18


title: HumanSpg-Seq6_C17


title: Ad-Id4GFP-Seq3_C09


title: Ad-Id4GFP-Seq3_C07


title: HumanSpg-Seq6_C20


title: Ad-Id4GFP-Seq3_C06


title: HumanSpg-Seq6_C16


title: Ad-Id4GFP-Seq3_C05


title: HumanSpg-Seq6_C15


title: Ad-Id4GFP-Seq3_C03


title: Ad-Id4GFP-Seq3_C02


title: HumanSpg-Seq6_C13


title: Ad-Id4GFP-Seq2_C95


title: Ad-Id4GFP-Seq2_C94


title: HumanSpg-Seq6_C11


title: HumanSpg-Seq6_C09


title: Ad-Id4GFP-Seq2_C96


title: Ad-Id4GFP-Seq2_C93


title: HumanSpg-Seq6_C24


title: HumanSpg-Seq6_C10


title: Ad-Id4GFP-Seq3_C08


title: Ad-Id4GFP-Seq2_C92


title: Ad-Id4GFP-Seq3_C12


title: HumanSpg-Seq6_C08


title: Ad-Id4GFP-Seq2_C91


title: HumanSpg-Seq6_C07


title: HumanSpg-Seq6_C06


title: HumanSpg-Seq6_C05


title: Ad-Id4GFP-Seq2_C89


title: HumanSpg-Seq6_C04


title: Ad-Id4GFP-Seq2_C90


title: Ad-Id4GFP-Seq2_C87


title: Ad-Id4GFP-Seq2_C88


title: HumanSpg-Seq6_C12


title: HumanSpg-Seq6_C03


title: Ad-Id4GFP-Seq2_C85


title: Ad-Id4GFP-Seq2_C86


title: HumanSpg-Seq5_C96


title: HumanSpg-Seq6_C02


title: Ad-Id4GFP-Seq2_C81


title: HumanSpg-Seq5_C93


title: Ad-Id4GFP-Seq2_C83


title: HumanSpg-Seq5_C95


title: HumanSpg-Seq5_C94


title: Ad-Id4GFP-Seq2_C80


title: Ad-Id4GFP-Seq2_C84


title: HumanSpg-Seq5_C91


title: Ad-Id4GFP-Seq2_C78


title: HumanSpg-Seq5_C92


title: HumanSpg-Seq5_C90


title: HumanSpg-Seq5_C88


title: Ad-Id4GFP-Seq2_C77


title: Ad-Id4GFP-Seq2_C76


title: HumanSpg-Seq5_C89


title: Ad-Id4GFP-Seq2_C79


title: HumanSpg-Seq5_C87


title: Ad-Id4GFP-Seq2_C75


title: HumanSpg-Seq6_C01


title: Ad-Id4GFP-Seq2_C73


title: HumanSpg-Seq5_C86


title: Ad-Id4GFP-Seq2_C71


title: Ad-Id4GFP-Seq2_C68


title: HumanSpg-Seq5_C83


title: HumanSpg-Seq5_C82


title: HumanSpg-Seq5_C81


title: Ad-Id4GFP-Seq2_C65


title: Ad-Id4GFP-Seq2_C69


title: Ad-Id4GFP-Seq2_C66


title: HumanSpg-Seq5_C80


title: HumanSpg-Seq5_C78


title: Ad-Id4GFP-Seq2_C63


title: Ad-Id4GFP-Seq2_C64


title: Ad-Id4GFP-Seq2_C62


title: HumanSpg-Seq5_C74


title: Ad-Id4GFP-Seq2_C60


title: HumanSpg-Seq5_C73


title: Ad-Id4GFP-Seq2_C59


title: Ad-Id4GFP-Seq2_C58


title: Ad-Id4GFP-Seq2_C57


title: HumanSpg-Seq5_C72


title: HumanSpg-Seq5_C70


title: Ad-Id4GFP-Seq2_C56


title: HumanSpg-Seq5_C84


title: Ad-Id4GFP-Seq2_C55


title: HumanSpg-Seq5_C69


title: HumanSpg-Seq5_C66


title: HumanSpg-Seq5_C67


title: HumanSpg-Seq5_C68


title: Ad-Id4GFP-Seq2_C52


title: Ad-Id4GFP-Seq2_C53


title: HumanSpg-Seq5_C65


title: Ad-Id4GFP-Seq2_C50


title: HumanSpg-Seq5_C63


title: HumanSpg-Seq5_C64


title: Ad-Id4GFP-Seq2_C49


title: HumanSpg-Seq5_C75


title: Ad-Id4GFP-Seq2_C61


title: Ad-Id4GFP-Seq2_C47


title: HumanSpg-Seq5_C61


title: Ad-Id4GFP-Seq2_C45


title: Ad-Id4GFP-Seq2_C44


title: HumanSpg-Seq5_C59


title: Ad-Id4GFP-Seq2_C43


title: Ad-Id4GFP-Seq2_C42


title: HumanSpg-Seq5_C57


title: Ad-Id4GFP-Seq2_C41


title: HumanSpg-Seq5_C56


title: Ad-Id4GFP-Seq2_C39


title: HumanSpg-Seq5_C53


title: Ad-Id4GFP-Seq2_C38


title: Ad-Id4GFP-Seq2_C36


title: Ad-Id4GFP-Seq2_C37


title: HumanSpg-Seq5_C52


title: HumanSpg-Seq5_C50


title: Ad-Id4GFP-Seq2_C35


title: HumanSpg-Seq5_C43


title: Ad-Id4GFP-Seq2_C34


title: Ad-Id4GFP-Seq2_C33


title: HumanSpg-Seq5_C42


title: HumanSpg-Seq5_C60


title: HumanSpg-Seq5_C58


title: Ad-Id4GFP-Seq2_C31


title: HumanSpg-Seq5_C41


title: Ad-Id4GFP-Seq2_C32


title: HumanSpg-Seq5_C55


title: HumanSpg-Seq5_C40


title: Ad-Id4GFP-Seq2_C30


title: HumanSpg-Seq5_C35


title: Ad-Id4GFP-Seq2_C27


title: Ad-Id4GFP-Seq2_C29


title: Ad-Id4GFP-Seq2_C26


title: HumanSpg-Seq5_C37


title: HumanSpg-Seq5_C32


title: Ad-Id4GFP-Seq2_C25


title: HumanSpg-Seq5_C49


title: HumanSpg-Seq5_C30


title: HumanSpg-Seq5_C28


title: HumanSpg-Seq5_C29


title: Ad-Id4GFP-Seq2_C24


title: HumanSpg-Seq5_C25


title: Ad-Id4GFP-Seq2_C23


title: HumanSpg-Seq5_C24


title: Ad-Id4GFP-Seq2_C22


title: HumanSpg-Seq5_C23


title: HumanSpg-Seq5_C21


title: HumanSpg-Seq5_C22


title: Ad-Id4GFP-Seq2_C20


title: HumanSpg-Seq5_C33


title: Ad-Id4GFP-Seq2_C18


title: HumanSpg-Seq5_C20


title: Ad-Id4GFP-Seq2_C19


title: HumanSpg-Seq5_C16


title: Ad-Id4GFP-Seq2_C16


title: Ad-Id4GFP-Seq2_C15


title: HumanSpg-Seq5_C10


title: Ad-Id4GFP-Seq2_C14


title: HumanSpg-Seq5_C15


title: Ad-Id4GFP-Seq2_C12


title: Ad-Id4GFP-Seq2_C10


title: HumanSpg-Seq5_C03


title: HumanSpg-Seq5_C02


title: Ad-Id4GFP-Seq2_C08


title: Ad-Id4GFP-Seq2_C06


title: Ad-Id4GFP-Seq2_C05


title: HumanSpg-Seq4_C88


title: Ad-Id4GFP-Seq2_C21


title: HumanSpg-Seq4_C86


title: Ad-Id4GFP-Seq2_C04


title: HumanSpg-Seq4_C84


title: HumanSpg-Seq5_C17


title: Ad-Id4GFP-Seq2_C03


title: Ad-Id4GFP-Seq2_C02


title: HumanSpg-Seq4_C83


title: HumanSpg-Seq4_C80


title: HumanSpg-Seq4_C81


title: Ad-Id4GFP-Seq1_C96


title: Ad-Id4GFP-Seq2_C01


title: Ad-Id4GFP-Seq2_C13


title: HumanSpg-Seq4_C79


title: Ad-Id4GFP-Seq1_C94


title: HumanSpg-Seq5_C07


title: HumanSpg-Seq4_C78


title: HumanSpg-Seq4_C77


title: Ad-Id4GFP-Seq1_C91


title: HumanSpg-Seq5_C06


title: Ad-Id4GFP-Seq1_C92


title: HumanSpg-Seq4_C76


title: HumanSpg-Seq4_C75


title: Ad-Id4GFP-Seq1_C89


title: HumanSpg-Seq4_C73


title: Ad-Id4GFP-Seq1_C88


title: HumanSpg-Seq4_C72


title: Ad-Id4GFP-Seq1_C86


title: HumanSpg-Seq4_C71


title: HumanSpg-Seq4_C70


title: Ad-Id4GFP-Seq1_C85


title: HumanSpg-Seq4_C68


title: HumanSpg-Seq4_C66


title: HumanSpg-Seq4_C65


title: HumanSpg-Seq4_C67


title: Ad-Id4GFP-Seq1_C80


title: Ad-Id4GFP-Seq1_C79


title: Ad-Id4GFP-Seq1_C81


title: Ad-Id4GFP-Seq1_C73


title: HumanSpg-Seq4_C62


title: Ad-Id4GFP-Seq1_C72


title: Ad-Id4GFP-Seq1_C74


title: HumanSpg-Seq4_C64


title: Ad-Id4GFP-Seq1_C76


title: Ad-Id4GFP-Seq1_C69


title: Ad-Id4GFP-Seq1_C90


title: Ad-Id4GFP-Seq1_C87


title: HumanSpg-Seq4_C59


title: HumanSpg-Seq4_C60


title: HumanSpg-Seq4_C56


title: Ad-Id4GFP-Seq1_C66


title: HumanSpg-Seq4_C55


title: HumanSpg-Seq4_C54


title: Ad-Id4GFP-Seq1_C62


title: HumanSpg-Seq4_C53


title: Ad-Id4GFP-Seq1_C64


title: Ad-Id4GFP-Seq1_C65


title: Ad-Id4GFP-Seq1_C61


title: Ad-Id4GFP-Seq1_C60


title: HumanSpg-Seq4_C61


title: HumanSpg-Seq4_C51


title: Ad-Id4GFP-Seq1_C57


title: HumanSpg-Seq4_C63


title: HumanSpg-Seq4_C44


title: Ad-Id4GFP-Seq1_C55


title: HumanSpg-Seq4_C45


title: Ad-Id4GFP-Seq1_C68


title: HumanSpg-Seq4_C43


title: Ad-Id4GFP-Seq1_C54


title: HumanSpg-Seq4_C41


title: Ad-Id4GFP-Seq1_C53


title: HumanSpg-Seq4_C42


title: HumanSpg-Seq4_C39


title: Ad-Id4GFP-Seq1_C50


title: HumanSpg-Seq4_C38


title: Ad-Id4GFP-Seq1_C46


title: Ad-Id4GFP-Seq1_C47


title: Ad-Id4GFP-Seq1_C45


title: HumanSpg-Seq4_C36


title: HumanSpg-Seq4_C35


title: Ad-Id4GFP-Seq1_C43


title: HumanSpg-Seq4_C34


title: HumanSpg-Seq4_C50


title: Ad-Id4GFP-Seq1_C44


title: Ad-Id4GFP-Seq1_C42


title: Ad-Id4GFP-Seq2_C28


title: HumanSpg-Seq4_C32


title: Ad-Id4GFP-Seq1_C41


title: Ad-Id4GFP-Seq1_C58


title: Ad-Id4GFP-Seq1_C40


title: HumanSpg-Seq4_C30


title: HumanSpg-Seq4_C29


title: Ad-Id4GFP-Seq1_C39


title: HumanSpg-Seq4_C24


title: HumanSpg-Seq4_C28


title: HumanSpg-Seq4_C23


title: Ad-Id4GFP-Seq1_C35


title: Ad-Id4GFP-Seq1_C37


title: HumanSpg-Seq4_C25


title: HumanSpg-Seq4_C33


title: Ad-Id4GFP-Seq1_C33


title: Ad-Id4GFP-Seq1_C32


title: HumanSpg-Seq4_C22


title: Ad-Id4GFP-Seq1_C31


title: HumanSpg-Seq4_C20


title: Ad-Id4GFP-Seq1_C30


title: HumanSpg-Seq4_C19


title: Ad-Id4GFP-Seq1_C28


title: HumanSpg-Seq4_C16


title: Ad-Id4GFP-Seq1_C26


title: HumanSpg-Seq4_C15


title: Ad-Id4GFP-Seq1_C25


title: HumanSpg-Seq4_C13


title: Ad-Id4GFP-Seq1_C24


title: HumanSpg-Seq4_C12


title: HumanSpg-Seq4_C11


title: HumanSpg-Seq4_C10


title: Ad-Id4GFP-Seq1_C23


title: HumanSpg-Seq4_C08


title: HumanSpg-Seq4_C07


title: Ad-Id4GFP-Seq1_C21


title: HumanSpg-Seq4_C21


title: Ad-Id4GFP-Seq1_C16


title: HumanSpg-Seq4_C06


title: Ad-Id4GFP-Seq1_C29


title: HumanSpg-Seq4_C02


title: Ad-Id4GFP-Seq1_C22


title: HumanSpg-Seq4_C05


title: Ad-Id4GFP-Seq1_C17


title: Ad-Id4GFP-Seq1_C27


title: Ad-Id4GFP-Seq1_C12


title: Ad-Id4GFP-Seq1_C11


title: Ad-Id4GFP-Seq1_C13


title: HumanSpg-Seq4_C01


title: HumanSpg-Seq4_C18


title: Ad-Id4GFP-Seq1_C15


title: HumanSpg-Seq3_C95


title: HumanSpg-Seq3_C94


title: HumanSpg-Seq3_C93


title: HumanSpg-Seq4_C09


title: Ad-Id4GFP-Seq1_C09


title: Ad-Id4GFP-Seq1_C06


title: HumanSpg-Seq3_C92


title: Ad-Id4GFP-Seq1_C05


title: Ad-Id4GFP-Seq1_C01


title: Ad-Id4GFP-Seq1_C20


title: HumanSpg-Seq3_C91


title: HumanSpg-Seq3_C89


title: HumanSpg-Seq3_C87


title: HumanSpg-Seq3_C85


title: HumanSpg-Seq3_C83


title: HumanSpg-Seq3_C84