title: 1001701405_I13


title: 1001701405_I16

status: Public on Nov 19 2018

type: SRA

city: Kensington

first: Fabio

last: Zanini

state: NSW

address: High and Botany St

country: Australia

institute: University of New South Wales

library_source: transcriptomic

data_processing:
bcl2fastq demux
STAR mapping
Htseq-count 0.7+ gene counting
Stampy virus mapping
Custom script for filtering virus reads
Genome_build: grch38
Supplementary_files_format_and_content: One TSV for each sample containing the counts of gene expression.

molecule: total RNA

organism: Homo sapiens

source_name: blood (PBMCs)

extract_protocol:
No extraction, single cells are sorted into 0.4ul lysis buffer
Cells are lysed in lysis buffer containing Triton X-100, then RT using template switching oligo and preamplification for 21 cycles as in SmartSeq2 is performed. Cells with different levels of intracellular virus are cherry picked and sequencing libraries are made using illumina’s Nextera XT kit.

tag: tissue

value: blood

tag: patient id

tag: cell type

value: monocyte

tag: Sex

value: F

tag: diseaseseverity

value: severe dengue

tag: numberhumanuniquelymappedreads

tag: numbervirusreads

tag: age


title: 1001702203_G4


title: 1001702203_G5


title: 1001701405_I12

value: NK cell


title: 1001702203_G3


title: 1001701405_I14


title: 1001701405_I11

value: B cell


title: IC_490

city: Hangzhou

first: Yishan

last: Chen

address: #866 Yuhangtang Road

country: China

institute: Zhejiang University

data_processing:
Illumina Casava1.7 software used for basecalling.
alignment was conducted with Tophat
The final gene expression data was normalized using Cuffnorm and represented as counts
Genome_build: mm9
Supplementary_files_format_and_content: Csv file includes raw counts of each gene from each sample

organism: Mus musculus

source_name: chemical-induced intermediate cells

growth_protocol: Before treatment, MEFs were cultured in 10% FBS+ H-DMEM at a confluency of 90%. Mchons were cultured in 10% FBS + DMEM/F12 a confluency of 80-90%

extract_protocol: Single cells were lysated, preamplified and library prepared according C1-HT IFC protocol of Fluidigm Corporation.

treatment_protocol: Ci-Ics: MEFs treated by chemical cocktails (VCRTs) under hypoxia condition (5% O2) for 6 days. Ci-chons: chondrogensis induction of ci-Ics for 28 days.

tag: strain

value: chemical-induced intermediate cells

tag: passages

tag: treatment

value: chemical cocktail VCRTc / hypoxia

tag: time


title: IC_491


title: 1001701405_I17


title: IC_488


title: 1001701405_I9

value: NKT cell


title: 1001701405_I10

value: T cell


title: IC_489


title: 1001702203_G22

value: unknown


title: 1001701405_I8


title: IC_487


title: 1001701405_I7


title: IC_486


title: 1001702203_G21


title: 1001701405_I5


title: 1001702203_G20


title: 1001701405_I4


title: IC_485


title: 1001701405_I3


title: 1001702203_G2


title: 1001701405_I2


title: 1001702203_G23


title: 1001702203_G18


title: 1001702203_G19


title: IC_483


title: 1001701405_I1


title: IC_482


title: 1001701405_H21


title: 1001701405_H23


title: 1001701405_H22


title: IC_481


title: 1001701405_H18


title: 1001702203_G16


title: 1001701405_H20


title: 1001702203_G17


title: 1001701405_I6


title: 1001701405_H17


title: 1001701405_H16


title: 1001701405_H19


title: IC_480


title: 1001702203_G15


title: 1001701405_H15


title: 1001701405_H14


title: IC_479


title: 1001702203_G14


title: 1001701405_H13


title: IC_478


title: IC_484


title: 1001702203_G13


title: 1001701405_H11


title: 1001701405_H12


title: IC_477


title: 1001701405_H10


title: IC_476


title: 1001702203_G12


title: IC_475


title: 1001701405_H8


title: 1001701405_H7


title: 1001701405_H6


title: 1001702203_G10


title: IC_474


title: 1001702203_G11


title: 1001702203_G1


title: 1001701405_H3


title: 1001702203_F9


title: IC_473


title: 1001701405_H2


title: 1001701405_H1


title: 1001701405_H5


title: 1001701405_G23


title: 1001702203_F8


title: IC_472


title: IC_471


title: 1001701405_G21


title: 1001702203_F7


title: 1001701405_G22


title: IC_470


title: 1001701405_H9


title: 1001702203_F6


title: 1001701405_G20


title: IC_469


title: 1001701405_G18


title: 1001701405_G16


title: 1001701405_G17


title: 1001701405_G19


title: IC_468


title: 1001702203_F5


title: 1001701405_G15


title: IC_467


title: 1001701405_H4


title: 1001701405_G13


title: 1001702203_F4


title: 1001702203_F3


title: 1001701405_G10


title: 1001701405_G12


title: IC_466


title: 1001701405_G11


title: 1001702203_F23


title: 1001701405_G9


title: 1001702203_F22


title: IC_465


title: IC_464


title: 1001701405_G8


title: 1001701405_G7


title: 1001702203_F21


title: IC_463


title: 1001701405_G6


title: 1001701405_G5


title: 1001702203_F20


title: IC_462


title: 1001701405_G4


title: 1001701405_G3


title: 1001702203_F2


title: 1001701405_G14


title: IC_461


title: 1001701405_G2


title: 1001702203_F18


title: 1001701405_G1


title: 1001702203_F19


title: IC_460


title: IC_458


title: 1001701405_F22


title: IC_459


title: 1001701405_F20


title: 1001701405_F19


title: 1001701405_F21


title: 1001701405_F23


title: 1001702203_F16


title: 1001702203_F17


title: IC_457


title: 1001702203_F15


title: IC_456


title: 1001701405_F15


title: 1001701405_F14


title: 1001701405_F13


title: IC_455


title: 1001702203_F14


title: 1001702203_F13


title: IC_454


title: 1001701405_F11


title: 1001701405_F9


title: 1001701405_F8


title: 1001701405_F10


title: 1001702203_F12


title: IC_452


title: 1001701405_F5


title: 1001701405_F7


title: 1001701405_F6


title: IC_451


title: 1001702203_F10


title: 1001701405_F4


title: 1001702203_F11


title: IC_453


title: 1001701405_F18


title: 1001701405_F17


title: 1001701405_F3


title: 1001701405_F16


title: IC_450


title: 1001701405_F2


title: 1001701405_F1


title: IC_449


title: 1001702203_E9


title: 1001701405_E23


title: 1001701405_E22


title: 1001702203_E8


title: IC_448


title: 1001702203_E7


title: 1001701405_E21


title: 1001701405_E20


title: IC_447


title: 1001701405_E19


title: IC_446


title: 1001702203_F1


title: 1001701405_E18


title: 1001702203_E5


title: 1001702203_E4


title: 1001701405_F12


title: 1001701405_E14


title: IC_444


title: 1001702203_E3


title: 1001701405_E12


title: IC_443


title: 1001701405_E15


title: 1001701405_E13


title: 1001702203_E23


title: 1001701405_E9


title: IC_442


title: 1001701405_E8


title: IC_441


title: 1001702203_E22


title: 1001702203_E6


title: 1001701405_E11


title: 1001701405_E6


title: 1001701405_E16


title: IC_440


title: 1001701405_E5


title: IC_445


title: 1001701405_E17


title: 1001702203_E20


title: 1001702203_E2


title: IC_439


title: IC_438


title: 1001701405_E1


title: 1001701405_E3


title: IC_437


title: 1001701405_D23


title: 1001701405_E10


title: 1001701405_E4


title: 1001702203_E18


title: 1001701405_D21


title: 1001702203_E19


title: 1001701405_D22


title: 1001701405_E7


title: IC_435


title: 1001701405_D18


title: 1001702203_E16


title: IC_434


title: 1001701405_D15


title: 1001701405_D17


title: 1001701405_E2


title: 1001701405_D16


title: IC_433


title: 1001701405_D14


title: IC_432


title: 1001702203_E21


title: 1001701405_D13


title: 1001701405_D12


title: 1001701405_D11


title: 1001701405_D10


title: 1001702203_E14


title: 1001702203_E13


title: 1001701405_D9


title: IC_436


title: 1001702203_E17


title: 1001701405_D7


title: 1001701405_D19


title: IC_429


title: IC_431


title: 1001702203_E15


title: 1001702203_E10


title: 1001701405_D6


title: IC_428


title: 1001701405_D5


title: 1001701405_D3


title: 1001701405_D4


title: 1001702203_E1


title: 1001701405_D2


title: 1001701405_D1


title: IC_427


title: 1001701405_D20


title: 1001701405_C23


title: IC_426


title: IC_430


title: IC_425


title: 1001701405_D8


title: 1001702203_E11


title: 1001702203_D7


title: 1001701405_C22


title: IC_424


title: 1001701405_C20


title: 1001702203_D6


title: IC_423


title: 1001701405_C18


title: 1001701405_C19


title: 1001701405_C17


title: 1001702203_D5


title: 1001701405_C16


title: IC_422


title: 1001702203_D9


title: 1001702203_D8


title: IC_421


title: 1001701405_C15


title: 1001701405_C12


title: 1001701405_C21


title: IC_420


title: 1001702203_D3


title: 1001701405_C11


title: 1001701405_C7


title: 1001702203_D22


title: 1001701405_C8


title: 1001701405_C10


title: IC_419


title: 1001701405_C6


title: 1001701405_C9


title: 1001702203_D21


title: IC_418


title: 1001702203_D23


title: 1001701405_C5


title: IC_417


title: IC_416


title: 1001702203_D20


title: 1001701405_C4


title: 1001702203_D4


title: 1001701405_C3


title: 1001701405_C14


title: 1001702203_D2


title: 1001702203_E12


title: 1001701405_C13


title: 1001701405_C2


title: IC_415


title: 1001702203_D19


title: 1001701405_B23


title: IC_414


title: 1001701405_C1


title: 1001701405_B22


title: 1001702203_D18


title: IC_413


title: 1001701405_B20


title: 1001702203_D17


title: 1001701405_B21


title: IC_412


title: 1001701405_B19


title: 1001701405_B18


title: 1001702203_D16


title: IC_411


title: 1001701405_B17


title: 1001701405_B15


title: 1001701405_B16


title: 1001702203_D14


title: 1001701405_B14


title: 1001701405_B13


title: IC_410


title: IC_409


title: 1001701405_B12


title: 1001702203_D13


title: 1001701405_B11


title: IC_408


title: 1001701405_B10


title: 1001702203_D12


title: IC_407


title: 1001701405_B9


title: 1001701405_B8


title: 1001701405_B7


title: IC_406


title: 1001702203_D11


title: 1001701405_B6


title: 1001701405_B5


title: 1001701405_B4


title: 1001701405_B3


title: 1001702203_D15


title: IC_405


title: IC_404


title: 1001701405_B2


title: 1001702203_C8


title: 1001702203_D10


title: 1001701405_A23


title: 1001702203_C9


title: 1001701405_B1


title: 1001702203_D1


title: 1001701405_A22


title: IC_401


title: 1001701405_A21


title: 1001702203_C7


title: 1001701405_A20


title: 1001701405_A19


title: 1001702203_C6


title: 1001701405_A18


title: IC_400


title: 1001701405_A17


title: 1001702203_C5


title: IC_399


title: 1001702203_C4


title: 1001701405_A15


title: 1001701405_A16


title: IC_403


title: IC_398


title: 1001701405_A14


title: IC_397


title: 1001701405_A13


title: 1001702203_C3


title: 1001701405_A11


title: 1001701405_A12


title: IC_402


title: 1001702203_C23


title: 1001702203_C22


title: IC_396


title: 1001701405_A9


title: 1001701405_A10


title: 1001701405_A8


title: 1001701405_A7


title: 1001701405_A6


title: IC_394


title: 1001702203_C21


title: IC_395


title: 1001701405_A5


title: 1001702203_C20


title: 1001701405_A4


title: 1001701405_A3


title: IC_393


title: 1001702203_C2


title: 1001701405_A2


title: 1001701405_A1


title: IC_392


title: 1001702203_C19


title: 1001701403_P22


title: 1001701403_P23


title: IC_390


title: 1001701403_P21


title: 1001702203_C18


title: 1001702203_C17


title: 1001701403_P20


title: IC_389


title: 1001701403_P19


title: 1001702203_C16


title: 1001702203_C15


title: IC_388


title: 1001701403_P16


title: 1001701403_P14


title: 1001701403_P15

value: pDC


title: 1001701403_P13


title: IC_386


title: 1001701403_P12


title: IC_387


title: 1001701403_P11


title: 1001701403_P17


title: IC_385


title: 1001702203_C13


title: 1001701403_P10


title: 1001701403_P18


title: IC_391


title: 1001702203_C14


title: 1001701403_P9


title: 1001702203_C12


title: IC_383


title: 1001702203_C11


title: 1001701403_P8


title: 1001701403_P6


title: 1001701403_P7


title: 1001702203_C10


title: 1001701403_P5


title: IC_382


title: 1001701403_P4


title: IC_381


title: 1001702203_B9


title: 1001701403_P2


title: 1001701403_P3


title: 1001702203_C1


title: 1001701403_P1


title: IC_380


title: 1001701403_O23


title: 1001702203_B8


title: IC_379


title: 1001701403_O22


title: 1001701403_O21


title: 1001702203_B7


title: 1001701403_O20


title: 1001701403_O19


title: 1001702203_B6


title: IC_384


title: IC_377


title: 1001701403_O18


title: 1001701403_O17


title: 1001701403_O16


title: 1001702203_B5


title: 1001701403_O15


title: IC_375


title: 1001701403_O13


title: 1001702203_B4


title: 1001702203_B3


title: 1001701403_O12


title: 1001701403_O10


title: IC_374


title: 1001701403_O11


title: 1001701403_O9


title: IC_373


title: 1001702203_B23


title: 1001702203_B22


title: 1001701403_O8


title: IC_378


title: IC_372


title: 1001702203_B21


title: 1001701403_O7


title: IC_371


title: 1001701403_O6


title: 1001702203_B20


title: 1001701403_O4


title: IC_370


title: 1001701403_O3


title: IC_376


title: 1001701403_O14


title: 1001701403_O2


title: IC_369


title: 1001701403_O1


title: 1001702203_B2


title: 1001702203_B19


title: 1001701403_N23


title: 1001701403_N22


title: IC_368


title: 1001701403_N21


title: 1001702203_B18


title: 1001701403_N19


title: 1001701403_N20


title: IC_366


title: 1001701403_N18


title: 1001702203_B17


title: 1001702203_B16


title: 1001701403_O5


title: 1001701403_N17


title: 1001701403_N16

value: cDC


title: IC_364


title: 1001701403_N14


title: 1001702203_B14


title: 1001701403_N13


title: 1001702203_B13


title: 1001701403_N12


title: IC_363


title: IC_362


title: 1001701403_N11


title: 1001702203_B12


title: 1001701403_N10


title: IC_367


title: 1001701403_N8


title: 1001702203_B11


title: 1001701403_N7


title: IC_365


title: IC_360


title: IC_359


title: 1001701403_N6


title: 1001701403_N5


title: 1001701403_N4


title: 1001701403_N15


title: IC_358


title: 1001702203_B10


title: 1001701403_N2


title: 1001702203_B15


title: 1001702203_B1


title: 1001701403_N1


title: 1001701403_N3


title: 1001702203_A9


title: IC_356


title: 1001701403_M22


title: 1001702203_A8


title: 1001701403_N9


title: IC_361


title: IC_357


title: IC_355


title: 1001702203_A7


title: 1001701403_M19


title: 1001702203_A6


title: 1001701403_M18


title: 1001701403_M17


title: 1001702203_A5


title: IC_353


title: 1001701403_M16


title: 1001702203_A4


title: 1001701403_M13


title: IC_352


title: 1001701403_M12


title: IC_351


title: 1001701403_M23


title: 1001701403_M14


title: 1001702203_A23


title: 1001701403_M11


title: 1001701403_M21


title: IC_350


title: 1001701403_M20


title: 1001702203_A22


title: 1001701403_M8


title: 1001701403_M9


title: 1001701403_M10


title: 1001701403_M7


title: IC_349


title: IC_354


title: 1001701403_M6


title: IC_348


title: 1001701403_M5


title: 1001702203_A20


title: 1001701403_M15


title: 1001702203_A3


title: 1001701403_M2


title: 1001701403_M4


title: 1001701403_M3


title: 1001701403_M1


title: 1001702203_A19


title: 1001701403_L22


title: IC_345


title: 1001702203_A18


title: 1001701403_L21


title: IC_344


title: 1001702203_A21


title: IC_343


title: 1001701403_L19


title: 1001701403_L18


title: IC_342


title: 1001702203_A17


title: 1001701403_L17


title: 1001701403_L20


title: 1001701403_L16


title: IC_341


title: 1001701403_L15


title: 1001702203_A15


title: IC_347


title: 1001701403_L14


title: 1001702203_A2


title: IC_346


title: 1001701403_L13


title: 1001701403_L23


title: IC_339


title: 1001701403_L10


title: 1001702203_A14


title: 1001701403_L12


title: 1001702203_A12


title: IC_338


title: 1001702203_A16


title: 1001701403_L8


title: 1001701403_L7


title: 1001702203_A11


title: IC_337


title: 1001702203_A10


title: 1001701403_L11


title: 1001701403_L9


title: 1001702203_A1


title: 1001701403_L6


title: IC_336


title: 1001701403_L3


title: 1001701403_L5


title: 1001701403_L2


title: IC_340


title: IC_335


title: IC_334


title: 1001701403_L1


title: 1001701403_K23


title: 1001702202_P9


title: 1001702203_A13


title: 1001702202_P8


title: 1001701403_K22


title: IC_333


title: 1001701403_K21


title: IC_332


title: 1001701403_K19


title: 1001702202_P7


title: 1001701403_K20


title: 1001701403_K18


title: 1001701403_K17


title: 1001702202_P6


title: 1001701403_L4


title: IC_331


title: 1001701403_K16


title: 1001702202_P4


title: 1001702202_P5


title: IC_330


title: IC_329


title: 1001701403_K13


title: 1001701403_K15


title: 1001701403_K14


title: 1001702202_P3


title: IC_328


title: 1001702202_P23


title: 1001701403_K11


title: 1001701403_K12


title: IC_327


title: 1001701403_K9


title: 1001702202_P22


title: 1001701403_K8


title: IC_326


title: 1001701403_K7


title: 1001702202_P21


title: 1001701403_K6


title: 1001701403_K5


title: IC_325


title: 1001702202_P20


title: IC_324


title: 1001701403_K4


title: 1001701403_K3


title: 1001701403_K2


title: 1001702202_P2


title: IC_323


title: 1001701403_K10


title: 1001702202_P19


title: IC_322


title: IC_321


title: 1001701403_K1


title: 1001701403_J23


title: 1001702202_P17


title: 1001701403_J19


title: 1001701403_J20


title: 1001701403_J18


title: 1001701403_J17


title: 1001702202_P16


title: 1001701403_J21


title: 1001702202_P18


title: 1001701403_J16


title: IC_319


title: 1001702202_P15


title: 1001701403_J15


title: IC_318


title: 1001701403_J14


title: 1001701403_J13


title: IC_317


title: 1001702202_P14


title: 1001701403_J12


title: IC_316


title: 1001701403_J11


title: 1001702202_P13


title: 1001702202_P12


title: 1001701403_J10


title: IC_320


title: 1001701403_J22


title: IC_315


title: 1001701403_J9


title: 1001701403_J8


title: 1001701403_J7


title: 1001701403_J4


title: 1001702202_P1


title: IC_313


title: 1001701403_J6


title: IC_314


title: 1001702202_P11


title: 1001702202_P10


title: 1001701403_J3


title: 1001701403_J5


title: IC_312


title: IC_311


title: 1001702202_O9


title: 1001701403_I23


title: 1001701403_J1


title: 1001701403_J2


title: 1001702202_O8


title: 1001701403_I21


title: 1001701403_I22


title: 1001701403_I20


title: 1001702202_O7


title: 1001702202_O6


title: IC_309


title: 1001702202_O5


title: 1001701403_I17


title: IC_308


title: 1001701403_I16


title: IC_307


title: 1001701403_I18


title: IC_306


title: 1001701403_I13


title: 1001701403_I19


title: 1001701403_I12


title: 1001702202_O3


title: 1001701403_I14


title: IC_305


title: 1001701403_I11


title: IC_304


title: 1001701403_I10


title: 1001701403_I15


title: IC_310


title: 1001702202_O4


title: 1001701403_I8


title: 1001702202_O22


title: 1001702202_O23


title: 1001701403_I9


title: IC_303


title: 1001701403_I7


title: IC_302


title: 1001701403_I6


title: 1001702202_O21


title: 1001701403_I5


title: 1001702202_O20


title: 1001701403_I2


title: 1001701403_I3


title: 1001701403_I4


title: 1001702202_O2


title: 1001702202_O19


title: 1001701403_I1


title: IC_300


title: 1001701403_H23


title: 1001701403_H22


title: 1001702202_O18


title: IC_299


title: 1001701403_H21


title: IC_298


title: 1001701403_H20


title: 1001701403_H19


title: IC_297


title: 1001702202_O17


title: 1001701403_H18


title: 1001701403_H17


title: 1001701403_H16


title: 1001702202_O16


title: IC_296


title: 1001701403_H15


title: 1001702202_O15


title: IC_295


title: 1001701403_H13


title: 1001702202_O14


title: 1001701403_H14


title: IC_294


title: 1001702202_O12


title: 1001701403_H9


title: IC_292


title: IC_293


title: IC_301


title: 1001702202_O11


title: 1001702202_O13


title: 1001701403_H8


title: 1001701403_H6


title: 1001701403_H10


title: IC_290


title: 1001701403_H5


title: 1001701403_H7


title: 1001702202_O10


title: 1001701403_H2


title: IC_291


title: 1001701403_H3


title: 1001701403_H4


title: IC_289


title: 1001701403_H1


title: 1001702202_N9


title: 1001701403_G23


title: IC_287


title: 1001701403_H12


title: 1001701403_G22


title: 1001701403_G21


title: 1001702202_N8


title: 1001701403_H11


title: 1001701403_G19


title: 1001701403_G20


title: 1001702202_N6


title: IC_286


title: 1001701403_G18


title: 1001702202_O1


title: 1001702202_N5


title: IC_285


title: 1001701403_G16


title: 1001701403_G15


title: IC_283


title: IC_284


title: 1001701403_G17


title: 1001702202_N4


title: 1001701403_G11


title: 1001701403_G12


title: IC_288


title: 1001702202_N23


title: IC_282


title: 1001702202_N22


title: 1001701403_G10


title: 1001701403_G8


title: 1001701403_G7


title: 1001702202_N7


title: 1001701403_G9


title: 1001701403_G6


title: IC_279


title: 1001702202_N21


title: 1001702202_N20


title: 1001701403_G5


title: 1001701403_G14


title: 1001702202_N3


title: 1001701403_G3


title: 1001701403_G4


title: 1001702202_N2


title: IC_278


title: 1001701403_G13


title: 1001701403_G2


title: 1001701403_G1


title: 1001702202_N19


title: IC_281


title: IC_277


title: 1001701403_F23


title: 1001701403_F21


title: 1001701403_F20


title: 1001702202_N17


title: IC_280


title: 1001701403_F19


title: 1001702202_N16


title: IC_274


title: IC_275


title: 1001701403_F18


title: IC_273


title: 1001701403_F17


title: 1001702202_N15


title: 1001701403_F15


title: 1001702202_N14


title: IC_272


title: IC_271


title: 1001701403_F13


title: 1001701403_F14


title: 1001701403_F12


title: IC_276


title: 1001701403_F22


title: 1001702202_N18


title: 1001702202_N13


title: IC_269


title: 1001701403_F11


title: IC_270


title: 1001702202_N12


title: 1001701403_F8


title: 1001701403_F7


title: IC_268


title: 1001701403_F4


title: 1001702202_N10


title: 1001701403_F5


title: 1001701403_F6


title: 1001701403_F16


title: 1001701403_F3


title: 1001702202_N1


title: 1001701403_F2


title: IC_266


title: 1001702202_M9


title: 1001702202_M8


title: 1001701403_F1


title: 1001701403_F9


title: 1001701403_F10


title: IC_264


title: 1001702202_M7


title: 1001701403_E20


title: IC_267


title: 1001701403_E23


title: 1001702202_M6


title: 1001701403_E18


title: 1001702202_N11


title: 1001701403_E21


title: IC_263


title: 1001701403_E17


title: 1001702202_M5


title: IC_261


title: 1001701403_E16


title: 1001701403_E15


title: 1001701403_E13


title: IC_265


title: IC_260


title: 1001702202_M23


title: IC_259


title: 1001702202_M3


title: 1001701403_E22


title: 1001701403_E11


title: 1001701403_E19


title: IC_258


title: 1001701403_E10


title: 1001702202_M22


title: 1001701403_E8


title: 1001701403_E9


title: IC_262


title: IC_257


title: 1001701403_E6


title: 1001702202_M21


title: IC_256


title: 1001701403_E5


title: 1001701403_E7


title: 1001701403_E14


title: 1001702202_M4


title: 1001701403_E3


title: 1001702202_M2


title: IC_255


title: 1001701403_E2


title: IC_254


title: 1001701403_E1


title: 1001702202_M19


title: 1001701403_D23


title: IC_252


title: 1001702202_M18


title: IC_253


title: 1001701403_D22


title: 1001702202_M20


title: 1001701403_D19


title: 1001702202_M17


title: 1001701403_D18


title: 1001701403_D20


title: 1001701403_D21


title: 1001702202_M16


title: 1001701403_D17


title: 1001701403_E4


title: IC_250


title: 1001701403_D15


title: 1001701403_D16


title: 1001702202_M15


title: 1001701403_D14


title: IC_249


title: 1001702202_M14


title: 1001701403_D13


title: IC_251


title: IC_248


title: 1001702202_M13


title: IC_247


title: 1001701403_D11


title: 1001701403_D12


title: 1001701403_D10


title: 1001701403_E12


title: 1001702202_M12


title: 1001701403_D9


title: 1001701403_D8


title: IC_245


title: 1001701403_D7


title: 1001701403_D6


title: 1001701403_D5


title: 1001702202_M10


title: IC_243


title: 1001701403_D2


title: 1001702202_L9


title: 1001701403_D3


title: 1001702202_L8


title: 1001701403_C23


title: 1001701403_D4


title: 1001702202_M1


title: 1001702202_L7


title: 1001701403_C22


title: IC_242


title: 1001701403_D1


title: 1001701403_C21


title: IC_240


title: 1001701403_C19


title: 1001701403_C18


title: IC_239


title: 1001701403_C17


title: 1001702202_M11


title: 1001702202_L4


title: IC_241


title: IC_237


title: 1001701403_C20


title: 1001701403_C15


title: 1001701403_C14


title: 1001701403_C12


title: IC_236


title: 1001701403_C13


title: 1001702202_L3


title: IC_238


title: 1001701403_C9


title: IC_246


title: IC_244


title: 1001702202_L23


title: 1001701403_C8


title: 1001701403_C11


title: IC_235


title: 1001702202_L6


title: 1001702202_L22


title: 1001701403_C6


title: 1001702202_L5


title: 1001702202_L21


title: 1001701403_C10


title: 1001702202_L20


title: 1001701403_C5


title: 1001701403_C3


title: IC_232


title: IC_234


title: 1001701403_C2


title: IC_231


title: 1001702202_L19


title: 1001702202_L2


title: 1001702202_L18


title: 1001701403_B21


title: IC_233


title: IC_229


title: 1001701403_B18


title: 1001701403_B22


title: 1001701403_C16


title: 1001701403_B20


title: 1001702202_L16


title: 1001701403_B17


title: IC_228


title: 1001701403_B14


title: 1001701403_B19


title: 1001701403_B16


title: IC_227


title: 1001702202_L15


title: IC_230


title: IC_226


title: IC_225


title: 1001701403_B23


title: 1001701403_B15


title: 1001701403_B10


title: 1001702202_L17


title: 1001702202_L14


title: 1001701403_B11


title: 1001701403_B12


title: 1001702202_L13


title: IC_224


title: 1001702202_L12


title: IC_223


title: 1001701403_B9


title: 1001701403_B7


title: 1001701403_B8


title: 1001701403_B5


title: 1001701403_B4


title: IC_221


title: 1001702202_L10


title: 1001701403_C7


title: 1001702202_L11


title: 1001702202_K9


title: 1001701403_B1


title: 1001701403_B6


title: 1001701403_C1


title: 1001702202_L1


title: IC_219


title: 1001701403_B3


title: IC_220


title: 1001702202_K8


title: 1001701403_B2


title: 1001701403_A23


title: 1001701403_A22


title: IC_218


title: 1001701403_A21


title: 1001701403_A18


title: IC_216


title: 1001702202_K6


title: 1001701403_A19


title: 1001702202_K7


title: IC_222


title: 1001701403_A17


title: 1001702202_K5


title: 1001702202_K4


title: 1001701403_A14


title: IC_217


title: IC_214


title: 1001701403_A13


title: 1001701403_A16


title: 1001701403_A15


title: 1001702202_K3


title: IC_213


title: 1001701403_A11


title: 1001701403_A12


title: 1001702202_K23


title: IC_212


title: 1001701403_A10


title: 1001701403_A9


title: 1001701403_A20


title: 1001702202_K22


title: 1001701403_A8


title: IC_211


title: 1001701403_A7


title: 1001702202_K21


title: 1001701403_A6


title: 1001702202_K20


title: 1001701403_A5


title: 1001701403_A3


title: IC_215


title: 1001702202_K2


title: IC_208


title: IC_209


title: 1001701403_A1


title: 1001701403_A2


title: 1001701401_P21


title: 1001701401_P22


title: 1001702202_K18


title: IC_207


title: IC_206


title: 1001701401_P20


title: 1001701401_P19


title: IC_205


title: 1001702202_K17


title: 1001701401_P18


title: 1001702202_K16


title: IC_204


title: 1001701401_P17


title: 1001701401_P16


title: 1001701403_A4


title: 1001701401_P15


title: 1001702202_K15


title: IC_203


title: 1001701401_P23


title: 1001702202_K19


title: 1001702202_K14


title: 1001701401_P14


title: IC_210


title: IC_201


title: 1001701401_P10


title: 1001701401_P9


title: IC_202


title: 1001701401_P13


title: 1001702202_K13


title: IC_200


title: 1001701401_P8


title: 1001702202_K12


title: IC_199


title: 1001701401_P7


title: 1001702202_K11


title: 1001701401_P5


title: 1001702202_K10


title: 1001702202_K1


title: 1001701401_P4


title: 1001701401_P3


title: 1001701403_B13


title: 1001701403_C4


title: 1001701401_P11


title: IC_194


title: IC_196


title: 1001701401_P2


title: 1001701401_P12


title: 1001701401_O22


title: 1001702202_J6


title: 1001701401_O18


title: IC_193


title: 1001701401_O19


title: 1001702202_J9


title: 1001701401_O21


title: 1001702202_J8


title: 1001701401_O17


title: 1001701401_P1


title: 1001702202_J5


title: IC_197


title: 1001701401_P6


title: IC_195


title: 1001701401_O16


title: 1001701401_O23


title: 1001701401_O14


title: 1001701401_O15


title: 1001702202_J4


title: IC_191


title: IC_198


title: IC_192


title: 1001702202_J3


title: IC_190


title: 1001702202_J7


title: 1001701401_O12


title: 1001701401_O20


title: 1001701401_O11


title: 1001701401_O13


title: 1001701401_O10


title: 1001702202_J23


title: 1001701401_O7


title: 1001701401_O6


title: IC_187


title: 1001701401_O8


title: IC_188


title: 1001702202_J20


title: 1001701401_O5


title: 1001702202_J21


title: IC_186


title: IC_185


title: 1001702202_J19


title: 1001701401_O4


title: 1001701401_O1


title: 1001701401_N23


title: 1001702202_J18


title: 1001701401_N22


title: IC_189


title: 1001702202_J22


title: 1001701401_N20


title: 1001701401_O9


title: IC_183


title: IC_182


title: 1001701401_N21


title: 1001701401_N19


title: IC_181


title: 1001701401_N18


title: 1001701401_O3


title: 1001701401_N17


title: 1001702202_J2


title: 1001701401_N15


title: 1001702202_J14


title: 1001702202_J15


title: 1001701401_O2


title: 1001701401_N16


title: 1001702202_J16


title: 1001701401_N13


title: IC_184


title: 1001701401_N14


title: 1001701401_N12


title: 1001701401_N11


title: 1001702202_J17


title: 1001701401_N10


title: IC_177


title: 1001701401_N9


title: IC_178


title: 1001701401_N8


title: 1001702202_J12


title: IC_180


title: 1001701401_N7


title: IC_176


title: 1001702202_J1


title: IC_179


title: 1001701401_N6


title: 1001702202_J10


title: 1001701401_N4


title: 1001701401_N3


title: 1001701401_N1


title: 1001701401_N2


title: IC_172


title: 1001702202_J13


title: IC_174


title: IC_173


title: 1001701401_M22


title: IC_175


title: 1001701401_N5


title: 1001701401_M21


title: 1001701401_M23


title: 1001702202_I8


title: 1001702202_I9


title: 1001702202_I7


title: 1001702202_J11


title: 1001701401_M19


title: 1001701401_M20


title: 1001702202_I6


title: IC_171


title: 1001701401_M17


title: 1001701401_M18


title: IC_170


title: 1001702202_I5


title: 1001701401_M15


title: 1001701401_M14


title: 1001701401_M16


title: 1001702202_I3


title: IC_169


title: 1001702202_I4


title: IC_168


title: IC_167


title: IC_166


title: 1001702202_I22


title: 1001701401_M9


title: 1001701401_M13


title: 1001701401_M12


title: IC_165


title: 1001701401_M11


title: 1001702202_I21


title: 1001701401_M7


title: IC_164


title: 1001701401_M6


title: 1001701401_M5


title: IC_163


title: 1001701401_M8


title: 1001701401_M3


title: 1001702202_I20


title: 1001701401_M4


title: 1001701401_M2


title: 1001702202_I2


title: 1001701401_L23


title: 1001701401_M1


title: 1001702202_I23


title: 1001701401_M10


title: 1001701401_L22


title: 1001701401_L21


title: 1001702202_I19


title: IC_161


title: 1001701401_L20


title: 1001702202_I18


title: IC_159


title: 1001702202_I16


title: 1001702202_I17


title: 1001701401_L17


title: IC_160


title: 1001701401_L18


title: 1001701401_L19


title: 1001701401_L15


title: IC_162


title: IC_157


title: IC_158


title: 1001702202_I15


title: 1001702202_I14


title: 1001701401_L14


title: 1001701401_L13


title: 1001702202_I13


title: IC_156


title: 1001701401_L16


title: IC_155


title: 1001701401_L11


title: 1001701401_L10


title: 1001701401_L8


title: 1001702202_I12


title: 1001701401_L9


title: IC_154


title: 1001702202_I11


title: 1001702202_I10


title: 1001701401_L5


title: IC_152


title: 1001701401_L3


title: 1001701401_L4


title: IC_151


title: 1001702202_I1


title: 1001702202_H9


title: 1001701401_L2


title: 1001701401_L1


title: 1001701401_K22


title: IC_149


title: 1001702202_H8


title: 1001701401_K23


title: 1001701401_K21


title: 1001702202_H7


title: IC_148


title: 1001701401_K20


title: 1001702202_H6


title: IC_150


title: 1001701401_K19


title: 1001701401_L7


title: 1001701401_K18


title: IC_147


title: IC_153


title: 1001701401_L6


title: 1001702202_H5


title: 1001701401_L12


title: 1001701401_K16


title: IC_146


title: 1001701401_K17


title: 1001702202_H4


title: 1001701401_K15


title: 1001701401_K13


title: 1001701401_K14


title: 1001702202_H3


title: IC_145


title: IC_143


title: 1001701401_K9


title: 1001701401_K12


title: 1001701401_K10


title: IC_144


title: 1001701401_K11


title: 1001702202_H22


title: 1001701401_K8


title: 1001702202_H23


title: 1001701401_K7


title: 1001702202_H21


title: IC_142


title: 1001701401_K6


title: IC_141


title: 1001701401_K5


title: 1001701401_K4


title: IC_140


title: 1001701401_K2


title: IC_139


title: 1001701401_K1


title: 1001702202_H2


title: 1001701401_K3


title: IC_138


title: 1001702202_H19


title: 1001701401_J23


title: IC_137


title: 1001701401_J21


title: 1001702202_H17


title: 1001701401_J20


title: 1001701401_J19


title: 1001702202_H20


title: 1001702202_H18


title: 1001702202_H16


title: 1001701401_J17


title: 1001701401_J18


title: IC_136


title: 1001701401_J16


title: IC_135


title: 1001702202_H15


title: 1001701401_J15


title: 1001701401_J13


title: 1001702202_H14


title: 1001701401_J14


title: 1001701401_J10


title: IC_132


title: IC_133


title: 1001701401_J12


title: IC_134


title: 1001702202_H12


title: 1001701401_J11


title: 1001702202_H13


title: 1001701401_J8


title: IC_131


title: 1001702202_H11


title: 1001701401_J22


title: 1001701401_J7


title: IC_130


title: 1001701401_J6


title: 1001702202_H10


title: 1001701401_J5


title: IC_129


title: 1001701401_J4


title: 1001701401_J3


title: 1001702202_H1


title: 1001701401_J2


title: 1001702202_G9


title: IC_128


title: 1001701401_J1


title: 1001701401_J9


title: 1001702202_G8


title: 1001701401_I22


title: IC_127


title: IC_126


title: 1001701401_I23


title: 1001701401_I20


title: IC_125


title: 1001701401_I21


title: 1001702202_G7


title: 1001701401_I17


title: 1001701401_I19


title: 1001701401_I16


title: 1001701401_I15


title: 1001702202_G5


title: IC_124


title: IC_123


title: 1001702202_G4


title: 1001701401_I14


title: IC_121


title: 1001702202_G3


title: IC_122


title: 1001701401_I12


title: 1001701401_I10


title: IC_120


title: 1001701401_I11


title: 1001702202_G6


title: 1001702202_G22


title: 1001701401_I8


title: IC_119


title: 1001701401_I9


title: 1001702202_G21


title: 1001702202_G20


title: 1001701401_I6


title: 1001701401_I7


title: IC_117


title: 1001701401_I18


title: 1001701401_I4


title: IC_118


title: 1001702202_G2


title: 1001701401_I3


title: 1001701401_I2


title: 1001701401_I13


title: 1001701401_H23


title: 1001702202_G23


title: IC_115


title: 1001701401_H22


title: IC_114


title: 1001701401_H21


title: 1001701401_H20


title: IC_113


title: 1001702202_G18


title: 1001701401_H18


title: 1001701401_H19


title: 1001701401_H17


title: 1001701401_I5


title: 1001702202_G15


title: IC_112


title: 1001702202_G16


title: 1001701401_H16


title: 1001701401_H14


title: IC_111


title: 1001701401_H15


title: IC_116


title: 1001702202_G14


title: 1001702202_G13


title: 1001702202_G19


title: IC_110


title: 1001701401_I1


title: IC_109


title: 1001701401_H11


title: 1001701401_H10


title: 1001701401_H12


title: 1001702202_G17


title: 1001701401_H8


title: IC_108


title: 1001702202_G11


title: IC_107


title: 1001702202_G10


title: 1001701401_H5


title: 1001701401_H6


title: IC_106


title: 1001701401_H4


title: 1001701401_H13


title: 1001702202_F9


title: 1001701401_H2


title: 1001701401_H3


title: IC_105


title: 1001702202_G1


title: IC_104


title: 1001702202_F8


title: 1001701401_G22


title: 1001701401_G23


title: IC_103


title: 1001701401_H9


title: 1001702202_G12


title: 1001701401_G21


title: 1001701401_G20


title: IC_102


title: 1001702202_F7


title: 1001701401_G19


title: 1001701401_H7


title: IC_100


title: 1001702202_F5


title: 1001701401_H1


title: 1001702202_F4


title: 1001701401_G13


title: IC_99


title: IC_97


title: 1001702202_F3


title: 1001701401_G11


title: 1001701401_G9


title: 1001701401_G14


title: 1001701401_G16


title: 1001702202_F23


title: 1001701401_G18


title: IC_98


title: IC_101


title: 1001701401_G15


title: 1001701401_G17


title: IC_96


title: 1001702202_F21


title: 1001701401_G7


title: 1001702202_F6


title: 1001701401_G6


title: 1001701401_G10


title: IC_94


title: 1001702202_F22


title: IC_93


title: 1001701401_G3


title: 1001702202_F20


title: 1001702202_F2


title: IC_95


title: 1001701401_F23


title: 1001702202_F19


title: 1001701401_G1


title: 1001701401_G4


title: 1001701401_F22


title: IC_91


title: IC_92


title: 1001702202_F18


title: 1001701401_G5


title: 1001701401_G12


title: 1001701401_F20


title: 1001702202_F17


title: 1001701401_F21


title: 1001701401_G2


title: 1001701401_F16


title: 1001702202_F16


title: 1001701401_F18


title: 1001701401_F14


title: IC_87


title: 1001702202_F15


title: 1001701401_F12


title: 1001702202_F14


title: 1001701401_F11


title: IC_88


title: 1001701401_F10


title: 1001701401_F13


title: 1001701401_F9


title: 1001701401_F15


title: 1001701401_F8


title: 1001701401_G8


title: 1001702202_F13


title: 1001701401_F7


title: 1001701401_F6


title: IC_90


title: 1001702202_F12


title: 1001701401_F5


title: 1001702202_F10


title: 1001702202_F11


title: 1001701401_F19


title: IC_85


title: IC_84


title: IC_83


title: 1001701401_F4


title: 1001702202_F1


title: 1001701401_F3


title: IC_82


title: IC_81


title: 1001701401_E23


title: 1001701401_F1


title: IC_80


title: IC_79


title: 1001701401_F2


title: 1001701401_E20


title: 1001701401_E22


title: 1001701401_E19


title: 1001701401_E21


title: IC_78


title: 1001701401_E18


title: 1001701401_E16


title: 1001702202_E8


title: 1001701401_E17


title: 1001702202_E5


title: IC_77


title: 1001701401_E15


title: 1001702202_E4


title: IC_76


title: 1001701401_E14


title: 1001702202_E6


title: 1001701401_E13


title: 1001702202_E9


title: 1001702202_E3


title: 1001701401_E12


title: IC_75


title: 1001702202_E23


title: IC_73


title: IC_89


title: 1001702202_E22


title: IC_72


title: 1001701401_E4


title: 1001701401_E9


title: 1001701401_E8


title: 1001701401_E10


title: 1001701401_E6


title: 1001701401_E11


title: 1001701401_E5


title: IC_71


title: 1001701401_E7


title: 1001701401_E3


title: IC_70


title: 1001701401_E1


title: 1001702202_E2


title: 1001701401_E2


title: 1001702202_E19


title: IC_69


title: 1001701401_D23


title: 1001701401_D22


title: IC_74


title: 1001701401_D21


title: 1001702202_E18


title: 1001702202_E21


title: IC_68


title: 1001702202_E17


title: IC_67


title: 1001702202_E16


title: 1001701401_D19


title: 1001702202_E15


title: 1001701401_D15


title: 1001701401_D16


title: 1001701401_D20


title: 1001702202_E20


title: 1001701401_D14


title: 1001701401_D17


title: 1001701401_D18


title: IC_65


title: 1001702202_E14


title: IC_66


title: IC_64


title: 1001701401_D12


title: 1001702202_E13


title: 1001701401_D13


title: 1001701401_D11


title: IC_63


title: 1001701401_D10


title: 1001702202_E12


title: 1001701401_D9


title: IC_62


title: 1001701401_D8


title: 1001702202_E11


title: IC_61


title: 1001701401_D7


title: IC_60


title: 1001701401_D3


title: IC_58


title: IC_59


title: 1001701401_D6


title: 1001701401_C23


title: 1001702202_D9


title: 1001701401_D4


title: 1001701401_C22


title: 1001701401_D5


title: 1001702202_E10


title: 1001702202_D8


title: 1001701401_D2


title: 1001702202_E1


title: 1001701401_C20


title: 1001702202_D7


title: IC_56


title: 1001701401_F17


title: IC_57


title: 1001701401_C19


title: 1001702202_D6


title: 1001701401_C21


title: 1001701401_C18


title: IC_55


title: 1001701401_C17


title: 1001702202_D5


title: 1001701401_C16


title: 1001701401_D1


title: IC_54


title: 1001702202_D4


title: 1001701401_C15


title: 1001701401_C13


title: 1001701401_C14


title: IC_53


title: 1001701401_C12


title: 1001702202_D3


title: 1001702202_D23


title: IC_52


title: 1001702202_D22


title: 1001702202_E7


title: IC_51


title: 1001701401_C10


title: IC_50


title: 1001701401_C9


title: 1001701401_C8


title: 1001701401_C7


title: IC_49


title: 1001702202_D21


title: 1001701401_C5


title: 1001701401_C6


title: IC_86


title: 1001701401_C4


title: IC_48


title: 1001701401_C3


title: 1001702202_D2


title: 1001701401_C2


title: 1001701401_C11


title: IC_47


title: IC_46


title: 1001702202_D19


title: 1001701401_B22


title: 1001701401_B23


title: 1001701401_B21


title: IC_45


title: 1001702202_D18


title: 1001701401_B19


title: 1001701401_B18


title: 1001702202_D17


title: 1001702202_D20


title: IC_44


title: IC_43


title: 1001701401_B15


title: 1001702202_D15


title: 1001701401_B17


title: 1001701401_B14


title: 1001701401_B16


title: IC_42


title: 1001702202_D14


title: IC_41


title: 1001701401_B13


title: 1001702202_D13


title: 1001701401_C1


title: 1001701401_B11


title: IC_40


title: 1001701401_B10


title: 1001702202_D12


title: 1001701401_B20


title: 1001702202_D16


title: 1001701401_B9


title: 1001701401_B8


title: IC_39


title: 1001702202_D11


title: 1001702202_D10


title: 1001701401_B7


title: 1001701401_B5


title: IC_38


title: 1001701401_B4


title: IC_35


title: IC_36


title: 1001701401_B6


title: 1001701401_A23


title: 1001701401_B12


title: 1001701401_B3


title: 1001702202_C9


title: IC_37


title: 1001701401_B1


title: 1001701401_B2


title: IC_34


title: 1001701401_A22


title: 1001701401_A21


title: 1001702202_C7


title: 1001702202_C8


title: 1001701401_A20


title: 1001702202_C6


title: IC_33


title: 1001701401_A19


title: 1001701401_A18


title: 1001701401_A17


title: IC_32


title: 1001702202_D1


title: 1001702202_C5


title: 1001701401_A16


title: IC_31


title: 1001701401_A14


title: IC_30


title: 1001701401_A15


title: 1001701401_A13


title: 1001701401_A12


title: IC_29


title: 1001701401_A11


title: 1001701401_A9


title: 1001702202_C4


title: IC_28


title: IC_27


title: 1001701401_A7


title: 1001701401_A8


title: 1001702202_C22


title: 1001701401_A10


title: IC_26


title: 1001701401_A6


title: 1001702202_C21


title: 1001702202_C20


title: 1001701401_A5


title: 1001701401_A4


title: IC_25


title: 1001702202_C2


title: 1001701401_A2


title: 1001701401_A3


title: 1001701401_A1


title: 1001702202_C19


title: 1001701305_P23

value: dengue fever


title: 1001701305_P22


title: 1001702202_C23


title: 1001702202_C3


title: 1001702202_C17


title: IC_22


title: 1001701305_P21


title: 1001701305_P18


title: IC_21


title: 1001701305_P19


title: 1001702202_C16


title: 1001701305_P20


title: IC_20


title: 1001701305_P16


title: IC_19


title: 1001702202_C15


title: 1001702202_C14


title: IC_24


title: IC_18


title: 1001701305_P13


title: IC_23


title: 1001701305_P12


title: 1001702202_C13


title: 1001702202_C18


title: 1001701305_P10


title: IC_16


title: 1001701305_P8


title: 1001702202_C11


title: 1001701305_P17


title: 1001701305_P7


title: 1001701305_P15


title: 1001701305_P5


title: 1001701305_P4


title: 1001701305_P14


title: 1001701305_P6


title: 1001702202_C10


title: IC_14


title: 1001701305_P2


title: 1001702202_C1


title: 1001701305_P3


title: IC_13


title: 1001701305_P1


title: 1001702202_B9


title: IC_12


title: 1001701305_P11


title: IC_17


title: 1001701305_P9


title: 1001701305_O22


title: IC_11


title: 1001702202_B8


title: 1001701305_O21


title: 1001702202_C12


title: 1001701305_O20


title: IC_15


title: IC_10


title: 1001701305_O18


title: 1001701305_O19


title: 1001701305_O17


title: 1001702202_B6


title: 1001702202_B5


title: 1001702202_B4


title: 1001701305_O15


title: IC_8


title: 1001701305_O16


title: 1001701305_O13


title: 1001702202_B3


title: 1001701305_O23


title: IC_6


title: 1001701305_O12


title: 1001701305_O14


title: 1001701305_O11


title: 1001702202_B23


title: 1001702202_B22


title: IC_9


title: IC_4


title: 1001701305_O10


title: 1001701305_O9


title: 1001702202_B7


title: IC_5


title: 1001701305_O8


title: 1001701305_O7


title: 1001702202_B21


title: 1001701305_O6


title: IC_7


title: 1001701305_O3


title: 1001701305_O2


title: 1001702202_B2


title: 1001701305_O1


title: IC_3


title: IC_2


title: IC_1


title: 1001701305_N23


title: 1001702202_B19


title: 1001701305_N21


title: 1001701305_N22


title: MEF_232

source_name: mouse embryonic fibroblasts

value: embryonic fibroblasts

value: growth medium


title: 1001701305_N20


title: 1001702202_B18


title: MEF_231


title: MEF_230


title: 1001701305_N16


title: 1001701305_N18


title: 1001701305_N19


title: 1001702202_B16


title: 1001702202_B17


title: 1001701305_N17


title: 1001701305_O4


title: 1001702202_B15


title: 1001702202_B20


title: MEF_228


title: 1001701305_N15


title: 1001701305_N13


title: 1001702202_B14


title: 1001701305_N14


title: MEF_227


title: 1001701305_N12


title: 1001701305_N11


title: 1001702202_B13


title: 1001701305_N10


title: MEF_225


title: 1001701305_N8


title: 1001701305_N7


title: MEF_224


title: 1001701305_N9


title: 1001702202_B11


title: MEF_229


title: 1001701305_N6


title: 1001702202_B10


title: MEF_223


title: 1001701305_N5


title: MEF_222


title: 1001702202_A9


title: 1001702202_B1


title: MEF_221


title: 1001701305_N1


title: 1001701305_N3


title: 1001701305_N2


title: MEF_226


title: 1001701305_M23


title: 1001702202_A8


title: 1001701305_M22


title: 1001701305_M21


title: 1001701305_O5


title: MEF_220


title: 1001702202_A7


title: 1001701305_M20


title: 1001702202_B12


title: MEF_219


title: 1001701305_M19


title: 1001701305_M18


title: MEF_218


title: 1001701305_M17


title: 1001701305_N4


title: 1001701305_M15


title: MEF_217


title: 1001701305_M16


title: 1001702202_A5


title: 1001701305_M14


title: 1001702202_A6


title: 1001702202_A4


title: MEF_216


title: 1001701305_M12


title: 1001701305_M11


title: 1001701305_M13


title: 1001702202_A3


title: 1001701305_M9


title: 1001701305_M10


title: 1001702202_A23


title: MEF_215


title: 1001701305_M8


title: MEF_214


title: MEF_213


title: 1001701305_M7


title: MEF_212


title: 1001702202_A21


title: 1001702202_A22


title: 1001701305_M6


title: 1001702202_A20


title: 1001701305_M4


title: 1001701305_M3


title: 1001701305_M2


title: 1001702202_A2


title: MEF_211


title: 1001702202_A19


title: MEF_210


title: 1001701305_M1


title: MEF_209


title: 1001701305_L23


title: 1001701305_L22


title: 1001701305_L21


title: 1001702202_A18


title: 1001701305_L20


title: 1001701305_M5


title: MEF_208


title: 1001701305_L18


title: 1001701305_L14


title: 1001701305_L15


title: 1001701305_L19


title: MEF_207


title: 1001701305_L16


title: 1001702202_A16


title: 1001702202_A15


title: MEF_204


title: MEF_206


title: 1001702202_A14


title: 1001701305_L11


title: MEF_205


title: MEF_203


title: 1001702202_A13


title: MEF_202


title: 1001701305_L9


title: 1001701305_L8


title: 1001702202_A12


title: 1001702202_A17


title: 1001701305_L7


title: MEF_201


title: 1001702202_A11


title: 1001701305_L6


title: 1001701305_L13


title: 1001701305_L5


title: MEF_200


title: 1001701305_L17


title: 1001701305_L3


title: 1001701305_L4


title: 1001702202_A10


title: MEF_199


title: 1001701305_L2


title: 1001701305_L12


title: 1001702202_A1


title: 1001702201_P9


title: MEF_198


title: 1001702201_P8


title: MEF_197


title: 1001702201_P7


title: 1001701305_K22


title: 1001702201_P6


title: MEF_196


title: 1001701305_K19


title: 1001701305_K20


title: MEF_195


title: 1001701305_K18


title: 1001701305_K17


title: 1001702201_P5


title: 1001701305_K16


title: 1001702201_P4


title: 1001701305_K15


title: 1001701305_L1


title: 1001701305_K14


title: 1001701305_K13


title: 1001701305_K12


title: 1001702201_P3


title: 1001701305_K23


title: 1001701305_K21


title: 1001701305_K10


title: MEF_192


title: 1001701305_K11


title: 1001701305_K9


title: 1001702201_P23


title: MEF_190


title: 1001701305_K8


title: MEF_191


title: 1001702201_P22


title: 1001701305_K7


title: 1001702201_P21


title: MEF_189


title: 1001701305_K6


title: 1001702201_P20


title: 1001701305_K5


title: 1001701305_K4


title: MEF_194


title: 1001701305_K3


title: MEF_188


title: MEF_193


title: 1001702201_P2


title: 1001702201_P19


title: 1001701305_K1


title: 1001701305_J23


title: MEF_187


title: 1001701305_K2


title: MEF_186


title: 1001701305_J22


title: 1001701305_J20


title: MEF_185


title: 1001701305_J19


title: MEF_184


title: 1001702201_P16


title: 1001701305_J17


title: 1001702201_P18


title: 1001701305_J21


title: 1001701305_J18


title: 1001702201_P17


title: 1001701305_J16


title: 1001702201_P15


title: 1001701305_J15


title: 1001701305_L10


title: MEF_183


title: 1001701305_J13


title: MEF_181


title: 1001702201_P14


title: 1001702201_P13


title: 1001701305_J12


title: 1001701305_J11


title: 1001701305_J10


title: MEF_179


title: 1001702201_P12


title: 1001701305_J9


title: MEF_180


title: 1001702201_P11


title: 1001701305_J7


title: MEF_178


title: 1001701305_J8


title: 1001702201_P10


title: MEF_177


title: MEF_182


title: 1001701305_J6


title: 1001701305_J4


title: 1001701305_J2


title: 1001701305_J1


title: MEF_175


title: 1001702201_P1


title: 1001701305_J14


title: 1001701305_I22


title: 1001702201_O8


title: MEF_174


title: 1001702201_O9


title: 1001701305_I21


title: 1001701305_I20


title: 1001702201_O7


title: MEF_173


title: 1001701305_I19


title: 1001702201_O6


title: MEF_172


title: 1001701305_J3


title: 1001701305_J5


title: MEF_176


title: 1001701305_I17


title: 1001702201_O5


title: 1001701305_I16


title: MEF_171


title: 1001701305_I18


title: 1001701305_I14


title: MEF_170


title: 1001701305_I23


title: 1001702201_O3


title: 1001701305_I15


title: MEF_169


title: 1001702201_O23


title: 1001701305_I13


title: 1001701305_I10


title: MEF_168


title: 1001701305_I11


title: 1001702201_O22


title: 1001701305_I9


title: 1001701305_I8


title: 1001702201_O20


title: 1001701305_I5


title: 1001702201_O4


title: 1001701305_I3


title: 1001701305_I4


title: MEF_165


title: 1001701305_I6


title: MEF_164


title: MEF_166


title: 1001702201_O2


title: 1001701305_I2


title: 1001701305_H23


title: 1001701305_I12


title: MEF_163


title: 1001701305_I1


title: 1001702201_O19


title: 1001702201_O17


title: 1001701305_H20


title: 1001701305_H19


title: 1001702201_O18


title: 1001701305_H21


title: MEF_162


title: 1001702201_O16


title: 1001701305_I7


title: MEF_167


title: MEF_160


title: 1001701305_H17


title: 1001702201_O21


title: 1001702201_O15


title: MEF_159


title: 1001701305_H22


title: 1001701305_H14


title: 1001702201_O13


title: MEF_158


title: MEF_157


title: 1001701305_H11


title: 1001701305_H10


title: 1001701305_H13


title: 1001701305_H12


title: MEF_161


title: 1001701305_H18


title: 1001702201_O11


title: 1001701305_H8


title: 1001701305_H9


title: 1001701305_H7


title: 1001701305_H15


title: 1001701305_H6


title: MEF_155


title: 1001702201_O12


title: 1001701305_H16


title: 1001701305_H5


title: 1001702201_O14


title: 1001701305_H4


title: MEF_154


title: 1001701305_H3


title: MEF_153


title: 1001702201_O1


title: 1001701305_H2


title: 1001702201_N9


title: MEF_152


title: 1001701305_H1


title: 1001702201_N8


title: MEF_156


title: 1001702201_N7


title: 1001701305_G22


title: 1001701305_G20


title: 1001701305_G21


title: 1001702201_O10


title: 1001701305_G23


title: 1001701305_G19


title: MEF_150


title: 1001701305_G17


title: MEF_151


title: MEF_149


title: 1001702201_N6


title: 1001701305_G18


title: 1001702201_N5


title: 1001702201_N4


title: MEF_147


title: 1001702201_N3


title: 1001701305_G13


title: 1001701305_G14


title: 1001701305_G15


title: 1001701305_G12


title: MEF_148


title: MEF_145


title: MEF_146


title: 1001701305_G11


title: 1001701305_G8


title: 1001702201_N23


title: 1001702201_N22


title: 1001701305_G16


title: 1001701305_G9


title: 1001701305_G6


title: 1001701305_G7


title: 1001701305_G5


title: 1001702201_N21


title: 1001702201_N20


title: 1001701305_G4


title: 1001701305_G2


title: 1001702201_N2


title: MEF_141


title: MEF_142


title: 1001702201_N19


title: 1001701305_G1


title: 1001701305_G10


title: 1001701305_F22


title: 1001702201_N18


title: MEF_139


title: MEF_144


title: 1001701305_F21


title: MEF_140


title: MEF_143


title: MEF_138


title: 1001702201_N17


title: 1001702201_N16


title: MEF_137


title: 1001701305_F19


title: 1001701305_F16


title: 1001701305_F20


title: 1001701305_F17


title: 1001701305_F18


title: 1001702201_N15


title: 1001701305_F15


title: 1001701305_G3


title: MEF_136


title: 1001701305_F14


title: 1001701305_F13


title: 1001702201_N14


title: MEF_135


title: 1001701305_F12


title: 1001701305_F11


title: 1001702201_N13


title: MEF_134


title: 1001701305_F10


title: 1001702201_N12


title: 1001701305_F9


title: MEF_133


title: 1001701305_F8


title: 1001702201_N11


title: 1001701305_F7


title: MEF_132


title: 1001702201_N10


title: 1001701305_F23


title: MEF_131


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title: MEF_89


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title: MEF_79


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title: MEF_76


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title: MEF_75


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title: MEF_62


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title: MEF_61


title: MEF_63


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title: MEF_56


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title: 1001702201_J3


title: MEF_54


title: mchon_281

source_name: mouse chondrocytes

value: limb cartilage

value: chondrocytes


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value: blood


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value: F


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value: severe dengue


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value: monocyte


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value: B cell


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title: 1001701303_N17


title: mchon_273


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value: NK cell


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title: 1001701303_J2


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title: 1001701303_I17


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title: 1001701303_I11


title: 1001701303_I13


title: mchon_214


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title: 1001701303_I12


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title: mchon_212


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title: 1001702201_G22


title: 1001701303_I8


title: 1001701303_I10


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title: mchon_211


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title: 1001702201_G2


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title: 1001701303_I1


title: 1001702201_G23


title: 1001701303_I4


title: mchon_207


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title: 1001702201_G18


title: 1001701101_M15

value: M

value: control


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title: 1001702201_G17


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title: 1001702201_G16


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value: T cell


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value: unknown


title: 1001701101_M4


title: 1001701101_M7


title: mchon_208


title: 1001702201_G12


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title: 1001701101_L5


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title: mchon_188


title: 1001702201_F21


title: 1001702201_F22


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title: 1001702201_F6


title: mchon_187


title: 1001701101_K23


title: 1001701101_L9


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title: 1001701101_K17


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title: 1001702201_F16


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title: mchon_176


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title: 1001702201_F1


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title: 1001701101_J23


title: 1001701101_K2


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title: 1001701101_K4


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title: 1001702201_E8


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title: 1001702201_E4


title: 1001701101_J9


title: mchon_168


title: mchon_169


title: 1001702201_E3


title: 1001701101_J4

value: NKT cell


title: 1001701101_J3


title: 1001702201_E22


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title: mchon_165


title: 1001701101_J1


title: 1001702201_E23


title: 1001702201_E20


title: mchon_164


title: 1001701101_I23


title: 1001702201_E21


title: 1001701101_I22


title: 1001701101_I20


title: mchon_163


title: 1001702201_E2


title: mchon_162


title: mchon_166


title: 1001701101_I15


title: 1001701101_I21


title: 1001701101_I17


title: 1001701101_I16


title: mchon_161


title: 1001701101_I14


title: 1001702201_E17


title: 1001701101_I13


title: 1001701101_J2


title: mchon_160


title: 1001701101_I12


title: 1001702201_E16


title: mchon_158


title: 1001701101_I18


title: 1001701101_I8


title: 1001702201_E19


title: mchon_157


title: 1001701101_I7


title: 1001701101_I10


title: 1001701101_I11


title: 1001702201_E14


title: 1001701101_I6


title: mchon_159


title: 1001702201_E15


title: 1001702201_E18


title: mchon_156


title: mchon_155


title: 1001702201_E13


title: 1001702201_E12


title: 1001701101_I9


title: 1001701101_H23


title: MEF_53


title: 1001702201_E11


title: 1001701101_H22


title: MEF_52


title: 1001701101_I1


title: 1001702201_E10


title: 1001701101_H21


title: MEF_50


title: 1001701101_H18


title: 1001701101_H19


title: 1001702201_E1


title: 1001701101_I3


title: 1001701101_H17


title: MEF_51


title: 1001701101_I5


title: 1001701101_I4


title: MEF_49


title: MEF_48


title: 1001701101_H16


title: 1001701101_I2


title: 1001701101_H20


title: 1001702201_D8


title: 1001702201_D7


title: 1001702201_D6


title: MEF_47


title: 1001701101_H11


title: 1001701101_H12


title: 1001701101_H13


title: 1001701101_H15


title: 1001702201_D9


title: 1001702201_D4


title: 1001701101_H10


title: 1001701101_H7


title: 1001701101_H8


title: 1001702201_D5


title: 1001702201_D3


title: MEF_45


title: 1001701101_H14


title: 1001702201_F13


title: 1001701101_H5


title: MEF_43


title: 1001701101_H6


title: 1001701101_H4


title: 1001702201_D23


title: 1001701101_H3


title: MEF_42


title: 1001701101_H2


title: MEF_46


title: 1001701101_H1


title: 1001702201_D22


title: MEF_41


title: 1001701101_H9


title: MEF_40


title: 1001701101_G21


title: 1001702201_D21


title: 1001701101_G22


title: MEF_44


title: 1001702201_D20


title: MEF_39


title: 1001701101_G18


title: 1001701101_I19


title: 1001701101_G20


title: MEF_38


title: 1001702201_D2


title: 1001701101_G17


title: 1001701101_G16


title: MEF_37


title: 1001701101_G15


title: 1001701101_K9


title: 1001702201_D19


title: 1001701101_G19


title: 1001702201_D18


title: 1001701101_G14


title: MEF_35


title: 1001701101_G23


title: MEF_36


title: 1001701101_G13


title: 1001701101_G11


title: 1001701101_G12


title: 1001701101_G10


title: MEF_34


title: 1001701101_G8


title: 1001701101_G9


title: 1001702201_D17


title: 1001702201_D16


title: 1001701101_G7


title: 1001702201_D15


title: 1001702201_D14


title: MEF_32


title: MEF_33


title: 1001701101_G6


title: 1001701101_G5


title: 1001701101_G4


title: MEF_31


title: 1001701101_G3


title: 1001701101_G2


title: 1001701101_F23


title: MEF_30


title: 1001702201_D12


title: 1001701101_G1


title: 1001701101_F21


title: 1001702201_D10


title: 1001702201_D1


title: 1001701101_F20


title: MEF_28


title: MEF_29


title: 1001701101_F19


title: 1001702201_C8


title: 1001702201_C9


title: 1001701101_F17


title: 1001701101_F18


title: mchon_154


title: 1001702201_C7


title: 1001701101_F16


title: mchon_153


title: 1001701101_F13


title: mchon_152


title: 1001701101_F12


title: 1001701101_F11


title: 1001702201_D11


title: 1001701101_F14


title: 1001702201_C6


title: mchon_151


title: 1001702201_D13


title: 1001701101_F15


title: 1001701101_F10


title: 1001701101_F22


title: 1001702201_C5


title: 1001701101_F9


title: mchon_150


title: 1001701101_F8


title: 1001702201_C4


title: MEF_27


title: 1001701101_F6


title: 1001701101_F7


title: mchon_149


title: 1001702201_C3


title: mchon_148


title: 1001701101_F5


title: 1001702201_C23


title: 1001701101_F4


title: mchon_147


title: 1001702201_C22


title: 1001701101_F3


title: 1001701101_F1


title: 1001701101_E22


title: 1001701101_E21


title: 1001701101_E20


title: 1001701101_E19


title: 1001702201_C20


title: 1001702201_C2


title: 1001702201_C21


title: 1001701101_E18


title: 1001701101_E23


title: mchon_143


title: mchon_145


title: mchon_146


title: mchon_144


title: 1001701101_E17


title: mchon_142


title: 1001702201_C19


title: 1001701101_E16


title: 1001701101_E15


title: 1001701101_E13


title: 1001702201_C18


title: 1001701101_F2


title: mchon_141


title: 1001701101_E11


title: 1001702201_C17


title: mchon_140


title: 1001701101_E14


title: 1001701101_E12


title: 1001702201_C16


title: 1001701101_E10


title: 1001701101_E7


title: mchon_139


title: 1001701101_E5


title: 1001701101_E9


title: JC40 SIV acute MesLN

city: Cleveland

first: Peter

middle: A

last: Wilkinson

state: Ohio

address: 2103 Cornell Road

department: Pathology / Systems Biology & Bioinformatics

country: USA

institute: Case Western Reserve University

data_processing:
fastq filtered for adapter and low quality bases using skewer/0.2.2
alignment to genome Mmul_8.0.1 using hisat2/2.0.3-beta
gene feature counting usng featureCounts from subread/1.5.0-p2
Genome_build: Mmul_8
Supplementary_files_format_and_content: counts.raw: counts as generated by featureCounts, rows are gene features, columns are samples
Supplementary_files_format_and_content: counts.normalized: remove counts that were all zeroes (across the full set; Perform TMM normalization ; Estimate GLM dispersion, export logCPM.

organism: Macaca mulatta

source_name: MesLN cell suspension (cryopreserved)

growth_protocol: in vivo

extract_protocol:
Qiagen micro rna extraction kit
TruSeq Stranded Total RNA – RiboZero Gold

tag: disease state

value: SIV

tag: condition

value: acute

value: mesenteric lymph nodes (MesLN)


title: 1001702201_C15


title: 1001701101_E8


title: 1001702201_C12


title: mchon_138

value: growth medium


title: 1001701101_E6


title: 1001702201_C14


title: 1001702201_C13


title: 1001702201_C11


title: 1001701101_E3


title: JC44 SIV chronic MesLN

value: chronic


title: 1001701101_E4


title: mchon_136

value: limb cartilage

value: chondrocytes


title: mchon_137


title: mchon_134


title: mchon_135


title: 1001701101_E2

value: blood


title: JC32 SIV acute MesLN


title: 1001702201_C10

value: F

value: severe dengue


title: JC28 SIV acute MesLN


title: JC36 SIV acute MesLN


title: 1001702201_C1


title: JC24 SIV chronic infected MesLN


title: 1001702201_B9


title: 1001701101_D23


title: mchon_131


title: 1001702201_B8


title: 1001701101_D22


title: JC20 healthy MesLN

value: healthy


title: mchon_130


title: JC16 SIV chronic infectedMesLN


title: 1001702201_B7


title: mchon_129


title: 1001701101_D21


title: JC08 SIV chronic infected MesLN


title: mchon_133


title: 1001702201_B6


title: 1001701101_D20


title: JC12 healthy MesLN


title: 1001701101_E1


title: mchon_128


title: 1001702201_B5


title: mchon_127


title: JC04 healthy MesLN


title: mchon_126


title: 1001701101_D18


title: 1001702201_B4


title: 1001702201_B3


title: 1001701101_D17


title: H3K27ac_ChIP-Seq UF 5 Input

city: Chicago

first: Matthew

last: Krause

state: Illinois

address: 5841 S. Maryland Ave

department: Medicine

institute: University of Chicago

library_source: genomic

data_processing:
Reads were aligned to hg19 with Bowtie2
Convert SAM to BAM using SAMtools
BAM files sorted and unmapped and mitochondrial reads were filtered out using SAMtools. Replicates coulb then be merged using SAMtools where appropriate.
Peaks called using MACS2 with input used as background control
Bedgraph files converted to bigWig or multiWig using HOMER
Genome_build: hg19
Supplementary_files_format_and_content: Fastq files contains raw reads. Bed files contain peaks called from MACS2

molecule: genomic DNA

source_name: Human Aortic Endothelial Cells

growth_protocol: Cells were grown in EGM-2 medium supplemented with SingleQuots from Lonza (CC-3156 & CC-4176) and Antibiotic-Antimycotic from Gibco (Grand Island, NY) (15240062) in a 37°C incubator with 5% CO2.

extract_protocol:
For ChIP, briefly, HAECs were fixed at room temperature with 1% paraformaldehyde in PBS for 10 min, and then quenched with glycine. Cells were collected by scraping.
To generate sequencing libraries for ChIP-seq, DNA ends were  repaired with T4 DNA Polymerase (Enzymatics #P7080L). Six-mer barcoded adapters  (BIOO Scientific NEXTflex #514104) were ligated with T4 DNA Ligase (Enzymatics #L-  6030-HC- L). Libraries were then amplified less than 14 cycles by PCR (Phusion Hot Start  II #F549L) and purified (Zymo #D5205) for high-throughput sequencing.

treatment_protocol: Flow was applied to cultured HAEC in a 6 well plate using a 1° tapered stainless steel cone held by a computerized stepper motor UMD-17 (Arcus Technology, Livermore CA). The cone recapitulated shear stress patterns corresponding to disturbed or unidirectional flow (Wu et al., 2015). The flow device was placed in a 37°C incubator with 5% CO2. HAECs at 100% confluence, maintained in EGM2- medium containing 4% dextran (Sigma-Aldrich, St. Louis, MO, 31392) in 6-well plates, were subjected to unidirectional flow or disturbed flow for 24 hours before cells being processed.

tag: flow

value: Unidirectional Flow

tag: passage

tag: cell id


title: mchon_132

tag: tissue


title: 1001702201_B23

tag: patient id


title: 1001701101_D16

tag: cell type

value: NK cell

value: M


title: mchon_124


title: H3K27ac_ChIP-Seq UF 4 Input


title: 1001701101_D15

tag: Sex

value: control


title: 1001702201_B22

value: T cell

tag: diseaseseverity


title: 1001702201_B21

tag: numberhumanuniquelymappedreads


title: mchon_123


title: 1001701101_D14

tag: numbervirusreads


title: mchon_122


title: H3K27ac_ChIP-Seq UF 3 Input


title: H3K27ac_ChIP-Seq DF 3 Input

value: Disturbed Flow


title: H3K27ac_ChIP-Seq DF 4 Input


title: 1001702201_B20

tag: age


title: 1001701101_D13


title: mchon_121

tag: strain


title: 1001702201_B2


title: mchon_120

tag: passages


title: 1001701101_D19


title: H3K27ac_ChIP-Seq UF 2 Input


title: mchon_119

tag: treatment


title: H3K27ac_ChIP-Seq DF 5 Input


title: 1001701101_D11

value: unknown


title: 1001702201_B19


title: H3K27ac_ChIP-Seq DF 2 Input


title: 1001702201_B18


title: mchon_125

tag: time


title: 1001701101_D10


title: H3K27ac_ChIP-Seq UF 1 Input


title: mchon_118


title: 1001702201_B17


title: 1001701101_D9


title: mchon_117


title: H3K27ac_ChIP-Seq DF 1 Input


title: 1001702201_B16

value: monocyte


title: 1001701101_D8


title: H3K27ac_ChIP-Seq UF 5


title: mchon_116


title: mchon_115


title: H3K27ac_ChIP-Seq DF 5


title: 1001701101_D7


title: mchon_114


title: 1001702201_B14


title: 1001702201_B15


title: 1001701101_D12


title: 1001701101_D6


title: 1001701101_D5


title: H3K27ac_ChIP-Seq UF 4


title: 1001702201_B13


title: 1001701101_D3


title: H3K27ac_ChIP-Seq DF 4


title: mchon_113


title: 1001701101_D4


title: 1001702201_B10


title: 1001702201_B12


title: 1001701101_D2


title: mchon_112


title: mchon_110


title: 1001702201_B1


title: 1001701101_D1


title: H3K27ac_ChIP-Seq UF 3


title: 1001702201_B11


title: mchon_108


title: 1001701101_C23


title: H3K27ac_ChIP-Seq UF 2


title: mchon_109


title: mchon_107


title: H3K27ac_ChIP-Seq UF 1


title: 1001702201_A7


title: 1001702201_A8


title: 1001702201_A9


title: H3K27ac_ChIP-Seq DF 1


title: mchon_111


title: H3K27ac_ChIP-Seq DF 3


title: mchon_106


title: mchon_104


title: 1001701101_C19


title: 1001701101_C21


title: 1001702201_A6


title: H3K27ac_ChIP-Seq DF 2


title: mchon_103


title: 1001701101_C22


title: ATAC-Seq J119 DF

data_processing:
Reads were aligned to hg19 with Bowtie2
Convert SAM to BAM using SAMtools
BAM files sorted and unmapped and mitochondrial reads were filtered out using SAMtools. Replicates coulb then be merged using SAMtools where appropriate.
Peaks called using MACS2
Bedgraph files converted to bigWig or multiWig using HOMER
Genome_build: hg19
Supplementary_files_format_and_content: Fastq files contains raw reads. Bed files contain peaks called from MACS2

extract_protocol:
For ATAC the cells were collected by trypsinizing and washed prior to being lysed.
ATAC-seq was performed as described in (Buenrostro et al., 2013) using Tn5 transposase (Illumina, San Diego CA).

value: UCLA J119


title: ATAC-Seq J119 UF


title: mchon_105

value: growth medium


title: ATAC-Seq R96 UF

value: UCLA R96


title: 1001702201_A3


title: 1001702201_A5


title: 1001701101_C17


title: mchon_102

value: limb cartilage


title: mchon_101

value: chondrocytes


title: 1001701101_C18

value: blood


title: 1001702201_A23

value: F


title: 1001701101_C16


title: ATAC-Seq R96 DF


title: mchon_100


title: 1001701101_C20


title: ATAC-Seq L101 DF

value: UCLA L101


title: 1001702201_A4

value: severe dengue


title: mchon_99


title: 1001702201_A21


title: 1001702201_A20


title: 1001701101_C13


title: ATAC-Seq L101 UF

value: Unidirectional Flow


title: mchon_98


title: 1001701101_C14

value: NK cell


title: ATAC-Seq HAEC UF


title: ATAC-Seq Y97 DF

value: UCLA Y97


title: ATAC-Seq HAEC DF


title: 1001702201_A19


title: 1001701101_C12

value: M

value: control


title: mchon_97


title: 1001702201_A2


title: ATAC-Seq Y97 UF


title: 1001702201_A22


title: 1001701101_C15


title: mchon_95


title: 1001701101_C10


title: ATAC-Seq HAEC Static 1

value: Static


title: mchon_96


title: mchon_94


title: 1001701101_C9

value: unknown


title: diestrus_6_serum

city: Lexington

first: Shavahn

middle: C

last: Loux

state: KY

address: 1400 Nicholasville

department: Veterinary Science

institute: University of Kentucky

data_processing:
The sequencing results were analyzed using an in-house small RNA analysis program - sRNAanalyzer (http://srnanalyzer.systemsbiology.net).  The adaptor sequences were trimmed and low-quality sequences such as low nucleotide complexity reads, homopolymer sequences or di-, tri-nucleotide repeat sequences were removed before the reads were mapped against various databases.
The processed reads were then sequentially mapped against databases including Equus caballus miRNA (mirBase), novel Equus caballus miRNA not yet incorporated in mirBase (horse_novel), transcripts, virus, plant and all miRNA (mirBase), coding transcripts (RefSeq), ribosomal and transfer RNA, equine non-coding RNA, equine coding transcripts (CDS) and equine genomic sequence (DNA)
Genome_build: EquCab 2.0
Supplementary_files_format_and_content: raw_read_counts.xls - excel file

organism: Equus caballus

extract_protocol:
The RNA derived from chorioallantois was precipitated, then quantified via spectrophotometry (NanoDrop 2000; Thermo Fisher Scientific).  Samples with a 260/280 ratio of 1.95 or greater, and a 260/230 ratio of 2.0 or greater underwent further analysis on the Bioanalzyer NanoChip (Agilent Technologies, Santa Clara, CA, USA)  to confirm the quality of the RNA.  Serum RNA was extracted from serum in a similar manner, with an initial starting ratio of 500 µL of serum to 1 mL of Trizol LS.  Quality and purity of the serum RNA was confirmed using the Bioanalyzer PicoChip.
Chorioallantois tissue libraries were created using NEBNext Multiplex Small RNA kit (New England Biolabs, Ipswitch, MA, USA) as per manufacturer’s instructions except where otherwise specified.  A total of 250 ng of chorionic RNA was used as input, with all ligations performed for 60 m at 25 ˚C.     The cDNA library was amplified using Realtime HiFi master mix (KAPA Biosystems, Wilmington, MA, USA), then amplified for 13 cycles as follows:  denatured at 94˚C for 15 s; annealed at 62˚C for 30 s; extended at 70˚C for 15 s, with a final extension at 70 ˚C for 5 m.  Samples were then cleaned using AmpureXP magnetic beads (Beckman Coulter Life sciences, Indianapolis, IN, USA), then run on a 6% DNA Retardation gel (LifeTechnologies, Carlsbad, CA, USA).   The 150 bp miRNA band was excised, eluted and precipitated in ethanol, then quantified by realtime PCR.  Serum RNA libraries were constructed in a similar fashion, although the entire volume of extracted RNA was used as starting material.   The NEBNext Multiplex Small RNA kit was used to create the cDNA library as described above, although the 3’ and 5’ adapters were diluted 1:10 prior to ligations.  The protocol was otherwise identical except serum libraries were amplified for 20 cycles instead of 13.

tag: gestational age

value: diestrus

value: serum


title: 1001701101_C8

value: T cell


title: 1001702201_A18


title: mchon_92

value: growth medium


title: 1001702201_A16


title: 1001702201_A17


title: diestrus_5_serum


title: mchon_91

value: limb cartilage


title: 1001701101_C7


title: 1001702201_A15


title: 1001701101_C6

value: monocyte


title: diestrus_3_serum


title: diestrus_4_serum


title: diestrus_2_serum


title: 1001702201_A12

value: blood


title: mchon_89

value: chondrocytes


title: 1001701101_C4


title: 1001701101_C11


title: 1001701101_C3


title: diestrus_1_serum


title: mchon_88


title: mchon_93


title: ATAC-Seq HAEC Static 2


title: 1001702201_A10

value: F


title: 1001702201_A13

value: severe dengue


title: 1001702201_A11


title: 1001701101_C2


title: postpartum_3_serum

value: postpartum


title: mchon_87


title: 1001702201_A1


title: 1001701101_C1


title: mchon_86


title: postpartum_2_serum


title: 1001702201_A14


title: mchon_90


title: mchon_85


title: postpartum_1_serum


title: 1001701101_C5


title: 10m_3_serum

source_name: 10 months_serum

value: 10 months


title: 1001701101_B23


title: 1001702104_P7


title: mchon_84


title: mchon_83


title: 10m_1_serum


title: 1001701101_B21


title: 1001701101_B22


title: 4m_3_serum

source_name: 4 months_serum

value: 4 months


title: 1001701101_B20

value: M


title: 1001702104_P6


title: mchon_82


title: 1001702104_P5


title: 10m_2_serum


title: 1001701101_B19

value: control


title: mchon_81


title: 4m_2_serum


title: mchon_80


title: 1001701101_B18


title: mchon_79


title: 1001701101_B17


title: 4m_1_serum


title: 1001702104_P4


title: 1001702104_P9


title: 1001702104_P8

value: B cell


title: 1001702104_P3


title: 1001702104_P23

value: unknown


title: postpartum_3_chorioallantois

value: chorioallantois


title: mchon_78


title: 1001701101_B16

value: NK cell


title: 1001701101_B15


title: postpartum_1_chorioallantois


title: mchon_77


title: 1001701101_B14


title: 10m_3_chorioallantois

source_name: 10 months_chorioallantois


title: mchon_76


title: mchon_75


title: 10m_2_chorioallantois


title: 1001702104_P20


title: 1001701101_B12


title: 10m_1_chorioallantois


title: 1001702104_P22


title: 1001702104_P2


title: mchon_73


title: 1001702104_P19


title: mchon_74


title: 4m_3_chorioallantois

source_name: 4 months_chorioallantois


title: 4m_2_chorioallantois


title: 1001702104_P18


title: 1001701101_B11


title: 1001701101_B9


title: postpartum_2_chorioallantois


title: 4m_1_chorioallantois


title: mchon_72


title: mchon_71


title: mchon_70


title: 1001702104_P21


title: 1001702104_P15


title: 1001702104_P16


title: 1001701101_B10


title: 1001701101_B7


title: 1001701101_B13


title: 1001702104_P14


title: 1001701101_B6

value: T cell


title: mchon_69

value: growth medium


title: mchon_68

value: limb cartilage


title: KO_Treg_aIRF4.fastq.gz

city: Onna-son,Kunigami-gun

first: Hiroki

last: Ishikawa

address: 1919-1 Tancha

country: Japan

institute: Okinawa Institute of Science and Technology Graduate University

data_processing:
Basecalls performed using RTA2
ChIP-seq reads were aligned to the mm9 genome assembly using Bowtie2.2.6 with optional parameters “-a --strata --best -m 1 -v 2”
peaks were called using Homer v4.8 with the default setting
Supplementary_files_format_and_content: bed files were generated using Homer v4.8
Genome_build: mm9
Supplementary_files_format_and_content: peaks bed

source_name: In vitro-activated Tregs

extract_protocol:
ChIP samples were prepared using a SimpleChIP Plus Enzymatic Chromatin IP Kit (9005S, Cell Signaling) as previously described. Treg cells (2-8 x 105 per ChIP-seq or 1-4 x 105 per ChIP-PCR) were activated with anti-CD3 antibody and anti-CD28 antibody in the presence of IL-2 for 60 h, crosslinked in culture medium containing 1% formaldehyde at room temperature for 10 min, and glycine solution was added to stop the reaction. After lysing cells, nuclei were isolated and treated with micrococcal nuclease (0.00313 mL mL-1) for 20 min at 37oC, and the reaction was stopped by adding 0.05 M EGTA. Samples were then sonicated to disrupt nuclear membranes and centrifuged to collect supernatants containing chromatin. Chromatin solutions were incubated with 1 mg of antibodies overnight at 4oC with rotation, and complexes of antibodies and chromatin were collected with Protein G magnetic beads (Veritas; DB10003). Beads were washed five times with low-salt wash solution and three times with high-salt wash solution buffer (incubated for 5 min for each washing) at 4oC. Chromatin was eluted, de-crosslinked following the manufacturer’s instructions, purified by phenol/chloroform extraction, and used for ChIP-sequencing .
To prepare ChIP-seq libraries, immunoprecipitated DNA was blunt-ended and ligated with adaptors using a KAPA Hyper Prep Kit (KAPA Biosystems; KK8500). DNA was then cleaned up with an Agencort AMPure XP (Beckman Coulter; A63880) at a 1.8x DNA ratio, amplified by PCR, and purified using the AMPure XP at a 1.2x DNA ratio. Library DNA was size-selected using a 2% agarose gel cassette of Blue Pippin (Sage Science) for a target size range 150-300 bp, quantified with droplet digital PCR (Bio-Rad), and then sequenced on an Illumina HiSeq 4000 to obtain 10 million uniquely aligned reads.

value: Treg

tag: genotype/variation

value: Foxp3cre JUNBFl/Fl

tag: antibody


title: WT_Treg_aIRF4.fastq.gz

value: Foxp3cre JUNB+/+

value: BATF


title: WT_Treg_aBATF.fastq.gz


title: 1001702104_P17

value: blood

value: F

value: severe dengue


title: mchon_67

value: chondrocytes


title: 1001701101_B5

value: M


title: 1001702104_P13

value: monocyte


title: 1001701101_B4

value: control


title: 1001702104_P12

value: unknown


title: WT_Treg_aJunB.fastq.gz

value: JunB


title: MEF_25

value: embryonic fibroblasts


title: 1001702104_P11


title: 1001701101_B3


title: MEF_26


title: KO3_Treg_S4_L001

data_processing:
Aligned to mouse genome using Tophat 2.2.1 with parameters “-r 100”
Cufflinks package was used for data normalization.
Genome_build: mm9
Supplementary_files_format_and_content: Expression_data

source_name: Tregs

extract_protocol:
Total RNA was isolated from FACS-sorted Treg cells using an RNeasy Plus Mini Kit (Qiagen; 74136).
RNA sequence libraries were prepared with NeoPrep (Illumina) using TruSeq Stranded mRNA NeoPrep Kit (Illumina). Libraries were then purified using Agencort AMPure XP (Beckman Coulter; A63880) at the ratio of 3:2 to remove adapter-dimer, then quantified with droplet digital PCR (Bio-Rad). Sequencing was performed on a HiSeq4000 (Illumina) with a HiSeq 3000/4000 SBS Kit (300 Cycles, Illumina; FC-410-1003) and a HiSeq 3000/4000 PE Cluster Kit (Illumina; PE-410-1001) to generate 150-nucleotide paired-end reads at a read depth of at least 20 million reads per sample.

value: Cd4cre JUNBFl/Fl


title: 1001702104_P10


title: KO2_Treg_S2_L001


title: MEF_24

value: growth medium


title: KO_Treg_S12


title: 1001702104_P1


title: 1001701101_B2

value: T cell


title: 1001701101_B1


title: MEF_23


title: WT3_Treg_S3_L001

value: Treg

value: JUNBFl/Fl


title: 1001702104_O9


title: 1001701101_B8


title: WT2_Treg_S1_L001


title: 1001701101_A23


title: MEF_22


title: 1001701101_A22


title: MEF_21


title: 1001702104_O7


title: 1001701101_A21


title: WT_Treg_S11