title: Medulloblastoma #379


title: Medulloblastoma #425

status: Public on Sep 02 2008

type: RNA

city: Amsterdam

first: Richard

last: Volckmann

state: Noord-Holland

address: van der Boechorststraat 6a

department: Laboratory of Experimental Oncology and Radiobiology

country: Netherlands

institute: msterdam UMC and University of Amsterdam

hyb_protocol: 10 μg of labeled cRNA was hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).

scan_protocol: Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer's protocol.

data_processing: Expression data (.cel files) were normalized with the MAS5 algorithm (target signal = 100) using GCOS software (Affymetrix). Data from the duplicate arraying experiments were averaged and further analyzed.

label: biotin

molecule: total RNA

organism: Homo sapiens

source_name: tumor

label_protocol: RNA sample integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies). 4 μg of total RNA was used for cRNA synthesis and fragmented. Labeling was performed with One-Cycle cDNA Synthesis Kit (Affymetrix) according to manufacturer's protocol. Sample quality was checked on a Bioanalyzer prior and after fragmentation.

extract_protocol: Total RNA was isolated using TRIzol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer's protocol.

tag: Gender

value: F

tag: Histology

value: Classic

tag: Metastatic stage


title: Medulloblastoma #421

value: M


title: Medulloblastoma #374

value: nd


title: Medulloblastoma #372


title: Medulloblastoma #373

value: LCA


title: Medulloblastoma #371


title: Medulloblastoma #336


title: Medulloblastoma #368


title: Medulloblastoma #370


title: Medulloblastoma #369


title: Medulloblastoma #377


title: Medulloblastoma #343


title: Medulloblastoma #340


title: Medulloblastoma #341


title: Medulloblastoma #342


title: Medulloblastoma #367


title: Medulloblastoma #365


title: Medulloblastoma #326


title: Medulloblastoma #317


title: Medulloblastoma #324


title: Medulloblastoma #272

value: Desmoplastic


title: Medulloblastoma #274


title: Medulloblastoma #273


title: Medulloblastoma #270


title: Medulloblastoma #275


title: Medulloblastoma #264


title: Medulloblastoma #269


title: Medulloblastoma #268


title: Medulloblastoma #261


title: Medulloblastoma #267


title: Medulloblastoma #265


title: Medulloblastoma #262


title: Medulloblastoma #259


title: Medulloblastoma #260


title: Medulloblastoma #258


title: Medulloblastoma #256


title: cerebellum_9

status: Public on Mar 30 2006

city: San Diego

middle: B

last: Roth

state: CA

address: 12790 El Camino Real

department: Molecular Medicine

country: USA

institute: Neurocrine Biosciences, Inc.

hyb_protocol: standard Affymetrix procedures

scan_protocol: standard Affymetrix procedures

data_processing: RMA

source_name: cerebellum

label_protocol: Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.

extract_protocol: Total RNA extracted using standard Trizol protocol, followed by Qiagen RNeasy column clean-up

tag: Normal tissue


title: cerebellum_5


title: cerebellum_1


title: cerebellum_8


title: cerebellum_10


title: cerebellum_7


title: cerebellum_4


title: cerebellum_3


title: cerebellum_2


title: brain, cerebellum: RNA#40_le1

status: Public on Jan 13 2006

city: Phoenix

first: Winnie

last: Liang

state: AZ

address: 445 N. Fifth Street

institute: Translational Genomics

description:
total RNA from biosource CC#40
Treatment, type=dissect, description=dissection from cerebellar tissue

source_name: brain, cerebellum


title: Medulloblastoma #255


title: brain, cerebellum: RNA#10S_le1

description:
total RNA from biosource CC#10S
Treatment, type=dissect, description=dissection from cerebellar tissue


title: brain, cerebellum: RNA#8S_le1

description:
total RNA from biosource CC#8S
Treatment, type=dissect, description=dissection from cerebellar tissue


title: brain, cerebellum: RNA#21*_le1

description:
total RNA from biosource CC#21*
Treatment, type=dissect, description=dissection from cerebellar tissue


title: brain, cerebellum: RNA#34*_le1

description:
total RNA from biosource CC#34*
Treatment, type=dissect, description=dissection from cerebellar tissue


title: brain, cerebellum: RNA#11*_le1

description:
total RNA from biosource CC#11*
Treatment, type=dissect, description=dissection from cerebellar tissue


title: brain, cerebellum: RNA#17_le1

description:
total RNA from biosource CC#17
Treatment, type=dissect, description=dissection from cerebellar tissue


title: brain, cerebellum: RNA#12_le1

description:
total RNA from biosource CC#12
Treatment, type=dissect, description=dissection from cerebellar tissue


title: brain, cerebellum: RNA#12S_le1

description:
total RNA from biosource CC#12S
Treatment, type=dissect, description=dissection from cerebellar tissue


title: brain, cerebellum: RNA#6S_le1

description:
total RNA from biosource CC#6S
Treatment, type=dissect, description=dissection from cerebellar tissue


title: brain, cerebellum: RNA#2S_le1

description:
total RNA from biosource CC#2S
Treatment, type=dissect, description=dissection from cerebellar tissue


title: brain, cerebellum: RNA#4S_le1

description:
total RNA from biosource CC#4S
Treatment, type=dissect, description=dissection from cerebellar tissue


title: brain, cerebellum: RNA#35_le1

description:
total RNA from biosource CC#35
Treatment, type=dissect, description=dissection from cerebellar tissue


title: brain, cerebellum: RNA#4*_le1

description:
total RNA from biosource CC#4*
Treatment, type=dissect, description=dissection from cerebellar tissue


title: NHGRI_UACC-1273

status: Public on Jan 24 2001

city: Bethesda

first: Michael

last: Bittner

state: MD

department: Cancer Genetics Branch

institute: NHGRI, NIH

description:
Biopsy location: axillary lymph node
Patient sex: male
Patient age: 50
Passage number: 16
p16 mutation status: mutation
Invasive ability: 2.5 +/- 0.3%
Vasculogenic mimicry: -
Gel contraction: -
Cell motility: ND
Scratch wound (%): 13

See http://www.nhgri.nih.gov/DIR/Microarray/Nature_paper.pdf
and http://www.nhgri.nih.gov/DIR/Microarray/Melanoma_Supplement/index.html

source_name: melanoma, lymph node