title: trSrebf1_3 (3'SAGE-seq)

extract_protocol:
Transferred lung fibroblasts were isolated from bleomycin-treated lungs of C57BL/6 mice at day 5 post-transfer. Lungs were cut into small pieces with a razor blade and digested enzymatically using 0.2% collagenase (Wako, Osaka, Japan), 0.96 mg/mL Dispase II (Roche, Basel, Switzerland), and 20 kU/mL DNase I (Sigma-Aldrich, St Louis, MO) for 60 min at 37°C. Erythrocytes were removed by 70% Percoll (GE Healthcare, Buckinghamshire, UK) density separation. The single-cell suspensions were then depleted of lineage+ (CD31+, CD45+, EpCAM+, CD146+ and Ter119+) cells by negative selection with an AutoMACS cell separator (Miltenyi Biotech, Bergisch Gladbach, Germany). Propidium iodide− lineage− Col1a2GFP+ hLNGFR+ live fibroblasts were further purified by cell sorting using a FACS Aria II (BD).
PolyA RNAs were isolated and amplified from donor fibroblasts according to the protocol described in (Huang H, Nucleic Actd Res, 2014) with some modifications. Briefly, 0.5 pmol of biotin-TEG-adapter-dT25 primers was bound to 20 μL of Dynabeads M270 streptavidin (Thermo Fisher Scientific). The washed beads (20 uL) were added to each cell lysis buffer containing 10,000 sorted fibroblasts and incubated for 30 min at room temperature with gentle rotation. Beads were washed once with wash buffer A [0.1% LiDS, 10 mM Tris-HCl (pH 7.5), 150 mM LiCl, and 1 mM EDTA] and three times with wash buffer B [10 mM Tris-HCl (pH 7.5), 150 mM LiCl, and 1 mM EDTA]. Beads were then suspended in 10 μL of RT mix 1 [1× SSIV buffer (Thermo Fisher Scientific), 2 mM dNTP, 2 M betaine (Sigma-Aldrich), 12 mM MgCl2, and 3.2 U/μL RNaseIn Plus (Promega)] and incubated for 90 s at 70ºC, 5 min at 35ºC, and immediately cooled on ice. RT mix 2 [10 μL; 1× SSIV buffer, 10 mM DTT (Thermo Fisher Scientific), 10 U/μL Superscript IV (Thermo Fisher Scientific), and 2 M betaine (Sigma-Aldrich)] was added, and reverse transcription was performed for 5 min at 35ºC and 15 min at 50ºC. Beads were washed once with cell lysis buffer, twice with B&W-T buffer [5 mM Tris-HCl (pH 7.5), 1 M NaCl, 0.5 mM EDTA, and 0.1% Tween-20], once with Tris-HCl (pH 8.0) and 20 μL of RNase H mix [1× first-strand buffer (Life Technologies, Carlsbad, CA, USA), 5 mM DTT, 0.6 U RNase H (Thermo Fisher Scientific)], and incubated for 20 min at 37ºC to digest reverse-transcribed mRNA. Beads were washed, 20 μL of TdT mix [50 mM Tris-HCl (pH 8.0), 100 mM KCl, 3 mM MgCl2, 1 mM CoCl2 (Roche), 0.65 mM dATP (Thermo Fisher Scientific), and 15.2 U/μL TdT (Roche)] was added on an ice-chilled aluminum rack, and polyA-tailing was performed for 3 min at 37ºC. The reaction was stopped by adding 5 μL of 0.5 M EDTA, and the enzyme was heat-inactivated by incubation for 10 min at 65ºC. Beads were washed, 20 μL of second-strand synthesis mix [1× KAPA Hifi ReadyMix (KAPA Biosystems, Wilmington, MA, USA) and 0.4 µM LNA anchored tagging primer] was added, and second-strand synthesis was performed according to the following program: 95ºC for 2 min, 98ºC for 20 s, 50ºC for 2 min, 72ºC for 7 min, and hold at 4ºC. Beads were washed, and 1/4 of the beads were used for the first round of whole-transcript amplification (WTA) in 25 μL of first-round WTA mix [1× KAPA Hifi ReadyMix (KAPA Biosystems), 0.4 µM anchored tagging primer, and 0.4 µM 3′ WTA primer] using the following program: 95ºC for 3 min, five cycles of 98ºC for 20 s, 65ºC for 15 s, and 72ºC for 7 min, followed by 72ºC for 5 min and a hold at 4ºC. PCR products were purified twice with 0.6× AmPure XP beads (Beckman Coulter) and eluted with 23 μL of nuclease-free water. Second-round WTA mix [27 μL; 1× KAPA Hifi ReadyMix (KAPA Biosystems), 0.614 µM 5′ WTA primer, and 0.614 µM Biotin-TEG-3′ WTA primer] was added, and the second round of WTA was performed using the following program: 95ºC for 3 min, nine cycles of 98ºC for 20 s, 65ºC for 15 s, and 72ºC for 7 min, followed by 72ºC for 5 min and a hold at 4ºC. PCR products were purified twice with 0.6× AmPure XP beads and eluted with 25 μL of Tris-HCl (pH 8.0). Amplified whole transcripts were quantified using a Nanodrop 1000 (Thermo Fisher Scientific), and size distribution was analyzed by agarose electrophoresis and SYBR Gold staining (Thermo Fisher Scientific).
3'SAGE-seq libraries were constructed according to the protocol described in (Matsumura H, Methods Mol Biol, 2012) with some modifications. Briefly, 150 ng of the whole-transcript library was restriction digested with NlaIII (New England Biolabs, Ipswich, MA, USA) for 2 h at 37ºC, and biotinylated 3′-tail transcripts were immobilized on Dynabead M-280 streptavidin beads (Thermo Fisher Scientific). Beads were washed, and 10 pmol of the CS1-EcoP15I-NlaIII adapter was ligated using a DNA ligation kit (Mighty Mix; Takara) for 30 min at 16ºC. Beads were washed three times with B&W-T buffer, once with 10 mM Tris-HCl (pH 8.0), suspended in 200 μL of EcoP15I digestion mix [1× NEBuffer 3.1, 1 mM ATP, and 0.2 U EcoP15I (New England Biolabs)], and digested for 16 h at 37ºC in a tightly sealed screw-cap tube with gentle rotation. Supernatant was purified using a Nucleospin Gel&PCR clean-up kit (Takara) and eluted twice with 12.5 μL of nuclease-free water. End-repair/polyA-tailing/ligation reactions were performed using NEBNext Ultra II modules (New England Biolabs) and 1.875 pmol of CS2-adapter according to manufacturer instructions. Reaction products were purified using a Qiagen MinElute Column (Qiagen, Hilden, Germany) and eluted with 13 μL of nuclease-free water. Barcoding mix [14.25 μL; 1× KAPA Hifi ReadyMix (KAPA Biosystems), 0.614 µM IonA-BC[N]-CS1-primer, and 0.614 µM Ion-trP1-CS2 primer] was added into 10.75 μL of elutant, and PCR enrichment was performed using the following program: 98ºC for 45 s, nine cycles of 98ºC for 15 s, 65ºC for 30 s, and 72ºC for 90 s, followed by 72ºC for 1 min and a hold at 4ºC. Reaction products were purified using double-size selection of AmPure XP beads (0.8× → 0.8×) and eluted with 10 μL of Tris-HCl (pH 8.0). The size distribution of each library was analyzed using an Agilent DNA high-sensitivity kit (Agilent Technologies, Santa Clara, CA, USA), and library concentration was quantified using a KAPA library quantification kit for Ion Torrent (KAPA Biosystems). Sequencing was performed by using Ion Hi-Q Chef Kit, Ion PI v3 Chip Kit and Ion Proton Sequencer (Thermo Fisher Scientific) according to the manufacture’s instructions except the input library concentration (100 pM) and cycle number (200).

treatment_protocol: C57BL/6 mice were anesthetized with isoflurane before a single dose of bleomycin sulfate (2 mg/kg dissolved in 50 μL of sterile saline solution; Toronto Research Chemical, Toronto, ON, Canada) was instilled by oropharyngeal aspiration. Primary fibroblasts were isolated from Col1a2-GFP murine lungs and active form of Srebf1c (trSrebf1) and control vector (marker : hLNGFR) were retrovirally transduced. 5x10^6 of each transduced cell were intratracheally-transferred to B6 mice at day 5 post-administration of 2 mg/kg of bleomycin. Expression of trSrebf1c was induced by doxycycline administration at day 7 post-administration of 2 mg/kg of bleomycin.

value: lineage (CD45, CD31, EpCAM, CD146 and Ter119) negative, propidium iodide negative, Col1a2-GFP and LNGFR positive fibroblast

tag: expression


title: trSrebf1_2 (3'SAGE-seq)

status: Public on Jan 30 2019

type: SRA

city: Chiba

first: Shigeyuki

last: Shichino

address: 2641, Yamasaki, Noda-shi

department: Department of Molecular regulation of Inflammatory and Immune Diseases

country: Japan

institute: Tokyo University of Science

library_source: transcriptomic

data_processing:
Adapter trimming and quality filtering of sequencing data were performed by using Trimommatic-v0.36 and PRINSEQ-0.20.4. Filtered reads were mapped to Refseq mm10 by using Bowtie2-2.2.5. Reads that were not mapped to NlaIII sites were removed, and quantified tag numbers of each gene as the expression level of each gene.
Between-sample normalization was performed by using Microsoft R Open 3.3.3 and TCC package.
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include raw tag-count values for each sample

molecule: polyA RNA

organism: Mus musculus

source_name: lung fibroblast

tag: strain

tag: genotype

value: Col1a2-GFP Transgenic

tag: tissue

value: lung

tag: cell type


title: zip-3 (gk3164) fed with spg-7 RNAi, biological rep1

city: Worcester

first: Cole

last: Haynes

state: Massachusetts

address: 364 Plantation Street, 425

country: USA

institute: U Mass Medical School

data_processing:
Illumina Casava1.7 software used for basecalling.
Sequenced reads were aligned to the C. Elegans genome (WBcel235) using STAR (version 2.5.3a) with the following parameter (--outFilterType BySJout \  --outFilterMultimapNmax 20  \  --alignSJoverhangMin 8  \  --alignSJDBoverhangMin 1  \  --outFilterMismatchNmax 999  \  --outFilterMismatchNoverReadLmax 0.04  \  --alignIntronMin 20  \  --alignIntronMax 1000000  \  --alignMatesGapMax 1000000  \)
featureCounts (subread-1.5.2) was used to count the features after the alignment
Bioconductor packages edgeR (version 3.10.0 ) was used for differential gene expression analysis
Genome_build: WBcel235 (https://www.ncbi.nlm.nih.gov/assembly/GCF_000002985.6/)
Supplementary_files_format_and_content: tab-delimited text files include the raw count values from featureCounts for each sample

molecule: total RNA

organism: Caenorhabditis elegans

source_name: zip-3 (gk3164) fed with spg-7 RNAi

growth_protocol: Wildtype, atfs-1(tm4919) and zip-3(gk3164) worms were synchronized by bleaching, raised to L4 on NGM plates seeded with control RNAi or spg-7 RNAi and then harvested.

extract_protocol:
Total RNA was isolated using the RNA STAT reagent (Tel-Test Inc) according to the manufacturer's instructions.
RNA libraries were prepared for sequencing using standard Illumina protocols

treatment_protocol: Worms were fed with either control RNAi or spg-7 RNAi

value: whole organism

tag: age


title: zip-3 (gk3164) fed with control RNAi, biological rep2

source_name: zip-3 (gk3164) fed with control RNAi


title: zip-3 (gk3164) fed with control RNAi, biological rep3


title: atfs-1 (tm4919) fed with control RNAi, biological rep3

source_name: atfs-1 (tm4919) fed with control RNAi


title: trSrebf1_1 (3'SAGE-seq)


title: control_3 (3'SAGE-seq)

value: control


title: control_2 (3'SAGE-seq)


title: wildtype fed with spg-7 RNAi, biological rep3

source_name: wildtype fed with spg-7 RNAi

value: wildtype


title: control_1 (3'SAGE-seq)


title: wildtype fed with spg-7 RNAi, biological rep1


title: wildtype fed with spg-7 RNAi, biological rep2


title: zip-3 (gk3164) fed with control RNAi, biological rep1


title: 15C3S4

type: RNA

city: Logan

first: John

middle: G.

last: Carman

state: UT

address: 4820 Old Main Hill

department: Plants, Soils and Climate Department

institute: Utah State University

hyb_protocol: Two color microarrays were synthesized from 12,000 35 nt probes by CombiMatrix (Irvine, CA) based on unigene EST from sorghum panicles. About 25 % of sorghum open reading frames were represented (Kim et al., 2005). No gap sequences with a ≥ 94 % BLASTN (Johnson et al., 2008) match (≥ 33 of 35 nt) to the S. bicolor V3.1.1 NCBI reference genome (McCormick et al., 2018) were retained (8664 of 12,000 probes). Annotations to arabidopsis and Oryza sativa L. (rice) were made using Phytozome 12.1.6 (Table S1). Labeled 3 µg aRNA samples were fragmented (Thermo Fisher reagents), verified by Nanodrop for concentration and labeling, and applied to 15 two-color arrays. A saturated loop design was used to distribute the 30 sib/stage/replication samples (2:3:5) evenly among the 15 arrays. Arrays were incubated at 45 ºC for 16 h. Files uploaded here include data from all 12000 probes even though only 8664 were used for downstream analyses.

scan_protocol: Arrays were scanned using a ScanArray Express HT microarray scanner (5 µm resolution).

data_processing: Median pixel intensities were captured using Imagene software (Biodiscovery, Inc., El Segundo, CA). Probe intensities were median normalized across samples. Normalized minimum or maximum values for each set of five replicate values (sib by stage by probe) that differed from the mean of the remaining four values by more than 2.5 times the standard deviation (remaining four values) were removed. Of the 51,984 sets of five replicate values, 47 % retained all five values after filtering, and all remaining sets of five replicate values contained four values. Normalized data were log 2 transformed, and differentially expressed genes (DEGs) (FDR ≤ 0.05) were identified by class comparison (BRB-ArrayTools 4.6.0, developed by Dr. Richard Simon and the BRB-ArrayTools Development Team, https://brb.nci.nih.gov/BRB-ArrayTools/). DEGs were located to sorghum chromosomes using Phytozome 12.1.6. Overrepresented gene ontology (GO) categories (Panther Overrepresentation test, released Nov. 13, 2018) were identified using arabidopsis orthologues of up or down regulated DEGs (Panther 13.1 release; Panther GO Ontology database, released Nov. 15, 2018). For Panther GO Enrichment analyses, expression data corresponding to arabidopsis orthologues were sorted by Log 2 values of fold change ratios. Where multiple probes represented the same arabidopsis gene, the most highly up or down regulated probe per gene was retained. Lists of enriched and overrepresented GO categories (p ≤ 0.025) were produced.

organism: Sorghum bicolor

source_name: Sex Ovule Meiocyte

label_protocol: RNA samples were dried in a Vacufuge Concentrator and amplified using Amino Allyl MessageAmp II kits (Thermo Fisher). Antisense RNA (aRNA) was Nanodrop and Bioanalyzer checked and stored at -80 ºC. aRNA samples (10 µg each) were Cy3 or Cy5 labeled and purified within 12 h of array hybridization.

extract_protocol: Ovules were excised from pistils in nuclease free water and placed in RNAlater. Dissection instruments and surfaces were cleaned periodically with RNaseZAP. Ovule samples were transferred to Microcon YM-100 Centrifugal Filter Columns containing 200 µL of lysis solution and centrifuged. Samples were rinsed with 100 µL RNase free water, centrifuged again, resuspended in 200 µL of lysis solution plus 20 µL Plant RNA Isolation Aid (Thermo Fisher), ground (on ice) for 2 min using RNAse-free pestles, centrifuged (12,500 x g, 2 min, 4 ºC), and ground for 1 min. Pestles were rinsed with 100 µL 100 % ethanol, which was collected in the grinding tubes. Samples were transferred to Micro Filter Cartridges, and total RNA was isolated using RNAqueous Micro kits (Thermo Fisher). RNA concentrations and quality were checked by Nanodrop 1000 spectrophotometry (Thermo Fisher) and by an Agilent 2100 Bioanalyzer with an RNA 6000 Nano Series II kit.

tag: ovule stage

value: Meiocyte


title: wildtype fed with control RNAi, biological rep2

source_name: wildtype fed with control RNAi


title: wildtype fed with control RNAi, biological rep1


title: wildtype fed with control RNAi, biological rep3


title: 15C5S6

source_name: Sex ovule ES

value: ES


title: 13C5S4

source_name: Sex ovule meiocyte

value: meiocyte


title: 10C3A6

source_name: Apo Ovule ES


title: 7C3S2

source_name: Sex Ovule MMC

value: MMC


title: 13C3S2


title: 10C5S2

source_name: Sex ovule MMC


title: 14C5S2


title: 12C5S6


title: 9C3S6

source_name: Sex Ovule ES


title: 12C3A6


title: 9C5A4

source_name: Apo ovule meiocyte


title: 11C5A6

source_name: Apo ovule ES


title: 8C5S4


title: 275_scfm2

city: Burlington

first: Korin

last: Eckstrom

state: VT

address: 89 Beaumont Ave

institute: University of Vermont

description: core_genome_raw_counts_1.24.19.csv (core genome analysis), accessory_counts_275_1.24.19.csv (accessory genome)

data_processing:
Basecalls performed with bcl2fastq v2.17.1.4
Reads were trimmed to remove adapters and low quality bases using Trimmomatic v 0.38 with the following parameters: clipping 3 bp from leading and trailing ends, sliding window of 4:15 and minimum length of 36 bp. Adapters were removed using the TruSeq SE file.
Read mapping and counting was performed using Rockhopper2, with default parameters and verbose output. The Stenotrophomonas maltophilia strain K279a (NC_010943.1) reference genome was used for the comparisons between all strains, while the individual strain comparisons used their respective draft genomes (273 = NZ_QTTF00000000.1 and 275 = NZ_QTTE00000000.1)
Raw counts from Rockhopper2 were anlayzed using the edgeR package in R. Only genes with at least 2 counts per million (cpm) and occured in at least 2 samples were included in subsequent analysis. Counts were adjusted for the library size of each sample and normalized by TMM transformation. A glm model of ~treatment + treatment:disease, where strains 273 and 275 are designated disease and strain K279a as bacteremia, was used to determine differential gene expression and deemed significant at a level of <0.05 FDR after Benjamini & Hochberg correction. Results were further filtered if they belonged to the core or accessory genome of each strain.
Core genome analysis was performed using the K279a genome as a reference to improve annotations and filtered to only include genes present in the SPINE defined core genome of the three strains. Accessory genome analyses were aligned to each strain's own genome (list here)
Genome_build: NC_010943.1, NZ_QTTF00000000.1, and NZ_QTTE00000000.1
Supplementary_files_format_and_content: comma-separated (.csv) files containing the raw counts

organism: Stenotrophomonas maltophilia

source_name: Bacterial cells

growth_protocol: Cultures were grown overnight at 37 ˚C in MOPS minimal media supplemented with 40 mM sodium lactate, 5 mM D-glucose, and 500 µM L-methionine. The following day, the cultures were collected through centrifugation, washed twice with 1 ml of MOPS media, and resuspended at a final concentration of 0.3 OD600 units in freshly prepared SCFM2 or MOPS media supplemented with 4 mM lactate and 250 µM L-methionine. Each strain was cultured in technical duplicate per condition for 4 h at 37 ˚C while shaking at 170 RPM. Technical duplicates were pooled and the data are presented from two inductions on different days

extract_protocol:
Cells were collected via centrifugation and immediately resuspended in 800 µl of ~80 C RNAzol. Total RNA was then extracted from these samples using Zymo Research’s RNA Miniprep Kit. Residual genomic DNA was removed from each sample through DNaseI treatment (NEB), and the RNA was re-purified using Qiagen’s RNeasy kit.
RNA was fragmented using the NEBNext Magnesium RNA Fragmentation Module, with a 3 minute incubation at 94°C, immediately followed by a cleanup step using the RNA Clean & Concentrator-5 (Zymo Research). Double-stranded, tagged cDNA was generated from 50 ng of fragmented RNA by the Peregrine method. RNA-seq was performed in biological duplicate for each strain and each condition

tag: clinical status

value: CF clinical sample

tag: growth condition


title: 275_lact2

value: Lactate


title: 275_lact1


title: 275_scfm1


title: 14C3S6


title: 8C3A4

source_name: Apo Ovule Meiocyte


title: 6C5A6


title: 11C3S4


title: 5C3A2

source_name: Apo Ovule MMC


title: 3C3A2


title: 6C3A4


title: 273_lact2

description: core_genome_raw_counts_1.24.19.csv (core genome analysis), accessory_counts_273_1.24.19.csv (accessory genome)


title: 4C3S4


title: 273_lact1


title: 273_scfm1


title: 273_scfm2


title: 7C5A4


title: 4C5A2

source_name: Apo ovule MMC


title: K279a_lact2

description: core_genome_raw_counts_1.24.19.csv (core genome analysis), accessory_counts_k279a_1.24.19.csv (accessory genome)

value: reference


title: K279a_lact1


title: 2C3A6


title: K279a_scfm1


title: SLX_13255.i710_i507

status: Public on Jan 29 2019

city: Nottingham

first: Fei

last: Sang

address: QMC

department: School of Life Sciences

country: United Kingdom

institute: University of Nottingham

description:
Pig_RawCounts.txt
Pig_TPMs.txt

data_processing:
Basecalls by Illumine Software Suite
Raw PE reads were trimmed against adaptor sequences by scythe (v0.981), and quality-trimmed by sickle (v1.33) using default settings.
Trimmed reads then were directionally aligned to Sus scrofa v10.2 by hisat2 (v2.1.0) with --know-splicestie-infile. Uniquely and correctly mapped reads were extracted for the downstream analysis.
htseq-count was used to count the number of reads aligned to each gene (Sus scrofa v10.2 ensembl annotation build 87).  Gene expression level was calculated and normalised by Transcripts Per Kilobase Million (TPM).
Genome_build: sus scrofa v10.2
Supplementary_files_format_and_content: tab-delimited text files for raw read counts and tpms of each gene, as well as rpms

organism: Sus scrofa

source_name: Pig embryo

growth_protocol: embryos were obtained from artificially inseminated pigs

extract_protocol:
Single cells were collected manually into lysis buffer following the SMARTSEQ. 2 protocol published by Picelli et al., 2014 (Nat. Protocols 9, 171-181)
Libraries were constructed using Nextera XT DNA Library Preparation Kit (Illumina), as used Picelli et al., 2014..

tag: embryo

value: Generic pig

value: Sph HYPO

tag: cell id


title: SLX_13254.i702_i504

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: K279a_scfm2


title: SLX_13257.i712_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13258.i702_i504

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13259.i704_i508

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13261.i711_i508

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13261.i712_i508

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13260.i706_i505

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13254.i702_i502

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13254.i701_i502

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13259.i701_i508

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13260.i704_i505

description:
Pig_RawCounts.txt
Pig_TPMs.txt

value: Sph EPI


title: SLX_13258.i711_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13259.i707_i508

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13258.i705_i504

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13254.i704_i504

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13259.i706_i508

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13258.i704_i504

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13256.i701_i504

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13257.i702_i508

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13255.i712_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13255.i711_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13260.i707_i505

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13257.i703_i508

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13256.i712_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13259.i705_i508

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13258.i703_i504

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: 5C5S6


title: SLX_13256.i711_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13254.i703_i504

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: 3C5S2


title: 2C5A2


title: SLX_13257.i701_i508

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13260.i703_i505

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13256.i710_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13255.i709_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13254.i701_i504

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13261.i708_i508

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13261.i709_i508

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: 1C5A4


title: SLX_13258.i712_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13259.i702_i508

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13256.i707_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13255.i706_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13255.i707_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13258.i701_i504

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13260.i708_i505

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13255.i708_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13257.i710_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13256.i709_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13259.i712_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13254.i704_i502

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13261.i707_i508

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13257.i707_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13256.i702_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt

value: LB HYPO


title: SLX_13258.i709_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13255.i704_i508

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13260.i707_i504

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13259.i711_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13256.i708_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13260.i701_i505

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13256.i706_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13255.i705_i508

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13257.i709_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13254.i703_i502

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13254.i712_i517

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13259.i705_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13259.i707_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13257.i703_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13260.i702_i505

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13255.i712_i506

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13256.i701_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: 1C3A2


title: SLX_13258.i710_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13260.i708_i504

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13257.i708_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13256.i712_i502

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13254.i708_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13259.i706_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13255.i711_i506

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13255.i710_i506

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13254.i711_i517

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13260.i706_i504

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13256.i709_i502

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13255.i701_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13255.i708_i506

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13256.i708_i502

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13255.i702_i508

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13258.i712_i502

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13254.i710_i517

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13260.i704_i504

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13256.i710_i502

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13260.i705_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13256.i711_i502

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13255.i709_i506

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13259.i702_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13258.i701_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13260.i705_i504

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13259.i703_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13257.i711_i506

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13261.i703_i508

description:
Pig_RawCounts.txt
Pig_TPMs.txt

value: LB EPI


title: SLX_13256.i705_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13260.i709_i504

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13259.i708_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13257.i704_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13258.i704_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13258.i706_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13254.i709_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13261.i704_i506

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13254.i711_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13259.i709_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13255.i703_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13258.i703_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13260.i711_i504

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13261.i704_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13256.i704_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13258.i708_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13257.i705_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13257.i706_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13260.i712_i504

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13260.i710_i504

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13255.i702_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13256.i703_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13259.i701_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt

value: EB TE


title: SLX_13257.i702_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13260.i703_i504

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13255.i706_i506

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13258.i711_i502

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13256.i707_i502

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13261.i706_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13257.i701_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13255.i705_i506

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13260.i710_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13260.i712_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13257.i708_i506

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13254.i712_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13259.i710_i506

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13257.i707_i506

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13261.i704_i502

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13255.i703_i506

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13261.i712_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13261.i711_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13257.i704_i506

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13255.i701_i506

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13258.i706_i502

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13261.i709_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13256.i703_i502

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13254.i706_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13259.i708_i506

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13259.i707_i506

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13254.i708_i517

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13258.i708_i502

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13261.i710_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt

value: EB ICM


title: SLX_13259.i709_i506

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13257.i703_i506

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13260.i701_i504

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13255.i704_i506

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13258.i710_i502

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13259.i712_i506

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13260.i709_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13255.i707_i506

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13258.i707_i502

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13259.i711_i506

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13256.i706_i502

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13255.i702_i506

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13258.i709_i502

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13261.i705_i502

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13261.i702_i502

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13255.i712_i505

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13254.i705_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13254.i704_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13254.i706_i517

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13254.i705_i517

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13258.i705_i502

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13254.i707_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13261.i705_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt

value: Morula


title: SLX_13257.i705_i506

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13261.i704_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13261.i706_i504

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13256.i701_i502

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13261.i708_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13255.i711_i505

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13256.i712_i517

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13254.i703_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13258.i703_i502

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13259.i704_i504

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13259.i705_i504

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13261.i705_i504

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13256.i710_i517

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13260.i706_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13260.i711_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13260.i707_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13255.i710_i505

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13261.i707_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13258.i702_i502

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13254.i701_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13257.i711_i505

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13256.i709_i517

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13260.i708_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13259.i706_i504

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13259.i703_i504

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13257.i702_i506

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13255.i708_i505

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13261.i706_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13254.i702_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13256.i708_i517

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13256.i711_i517

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13261.i705_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13260.i703_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13260.i704_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13259.i702_i504

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13254.i704_i517

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13258.i711_i517

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13259.i701_i504

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13255.i705_i505

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13255.i707_i505

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13257.i710_i505

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13256.i707_i517

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13257.i709_i505

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13256.i706_i517

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13255.i706_i505

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13259.i712_i505

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13255.i704_i507

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13258.i710_i517

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SLX_13258.i705_i503

description:
Pig_RawCounts.txt
Pig_TPMs.txt


title: SAMPLE 11_Raw WT-2

city: Xinxiang

first: Rong

last: Huang

address: 601 Jinsui Road

country: China

institute: Xinxiang Medical University

description: Gene expression data from Wildtypet Raw264.7cells.

hyb_protocol: Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45℃ on Affymetrix GeneChip® Mouse Genome 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.

scan_protocol: GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000

data_processing: The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.

label: biotin

label_protocol: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)

growth_protocol: Raw264.7 macrophages were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) containing 10% heat-inactivated fetal bovine serum (FBS), 100 units/ml penicillin, 100 µg/ml streptomycin.

extract_protocol: Trizol extraction of total RNA was performed according to the manufacturer's instructions

treatment_protocol: No treatment.

tag: cell line

value: macrophages

tag: genotype/variation

value: Wildtype


title: SAMPLE 9_Raw Vav3-3

description: Gene expression data from Vav3 Knockout Raw264.7cells.

source_name: Raw264.7_Vav3 Knockout

value: Vav3 Knockout


title: SAMPLE 4_Raw Vav2-1

description: Gene expression data from Vav2 Knockout Raw264.7cells.

source_name: Raw264.7_Vav2 Knockout

value: Vav2 Knockout


title: SAMPLE 12_Raw WT-3


title: SAMPLE 8_Raw Vav3-2


title: SAMPLE 5_Raw Vav2-2


title: SAMPLE 2_Raw Vav1-2

description: Gene expression data from Vav1 Knockout Raw264.7cells.

source_name: Raw264.7_Vav1 Knockout

value: Vav1 Knockout


title: SAMPLE 6_Raw Vav2-3


title: SAMPLE 1_Raw Vav1-1


title: SAMPLE 7_Raw Vav3-1


title: SAMPLE 10_Raw WT-1


title: WT_PD8_biological rep 02

city: Toyoake

first: Hideo

last: Hagihara

address: 1-98 Dengakugakubo, Kutsukake-cho

department: Institute for Comprehensive Medical Science

institute: Fujita Health University

description:
WT_PD8_biological rep 02
Gene expression data from the DG of wild-type mouse.

hyb_protocol: Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.

scan_protocol: GeneChips were scanned using Affymetrix GeneChip Scanner 3000 (GCS3000).

data_processing: The data were analyzed with Affymetrix Expression Console (version 1.1). The normalization used in RMA is quantile normalization.

source_name: DG of wild-type mouse

label_protocol: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA.

growth_protocol: No

extract_protocol: Trizol extraction of total RNA and cleanup by RNeasy Micro kit (QIAGEN) was performed according to the manufacturer's instructions.

treatment_protocol: No

tag: strain/background

value: C57BL/6J x BALB/cA

value: Wild-type

value: Dentate gyrus

value: Postnatal day 8


title: WT_PD25_biological rep 03

description:
WT_PD25_biological rep 03
Gene expression data from the DG of wild-type mouse.

value: Postnatal day 25


title: WT_PD17_biological rep 05

description:
WT_PD17_biological rep 05
Gene expression data from the DG of wild-type mouse.

value: Postnatal day 17


title: WT_PD8_biological rep 04

description:
WT_PD8_biological rep 04
Gene expression data from the DG of wild-type mouse.


title: WT_PD25_biological rep 02

description:
WT_PD25_biological rep 02
Gene expression data from the DG of wild-type mouse.


title: WT_PD25_biological rep 04

description:
WT_PD25_biological rep 04
Gene expression data from the DG of wild-type mouse.


title: WT_PD11_biological rep 03

description:
WT_PD11_biological rep 03
Gene expression data from the DG of wild-type mouse.

value: Postnatal day 11


title: WT_PD8_biological rep 05

description:
WT_PD8_biological rep 05
Gene expression data from the DG of wild-type mouse.


title: WT_PD17_biological rep 03

description:
WT_PD17_biological rep 03
Gene expression data from the DG of wild-type mouse.


title: WT_PD8_biological rep 03

description:
WT_PD8_biological rep 03
Gene expression data from the DG of wild-type mouse.


title: WT_PD11_biological rep 01

description:
WT_PD11_biological rep 01
Gene expression data from the DG of wild-type mouse.


title: WT_PD8_biological rep 01

description:
WT_PD8_biological rep 01
Gene expression data from the DG of wild-type mouse.


title: WT_PD11_biological rep 04

description:
WT_PD11_biological rep 04
Gene expression data from the DG of wild-type mouse.


title: WT_PD25_biological rep 05

description:
WT_PD25_biological rep 05
Gene expression data from the DG of wild-type mouse.


title: WT_PD17_biological rep 04

description:
WT_PD17_biological rep 04
Gene expression data from the DG of wild-type mouse.


title: WT_PD25_biological rep 01

description:
WT_PD25_biological rep 01
Gene expression data from the DG of wild-type mouse.


title: WT_PD11_biological rep 05

description:
WT_PD11_biological rep 05
Gene expression data from the DG of wild-type mouse.


title: WT_PD11_biological rep 02

description:
WT_PD11_biological rep 02
Gene expression data from the DG of wild-type mouse.


title: SAMPLE 3_Raw Vav1-3

description: Gene expression data from Vav1 Knockout Raw264.7cells.


title: WT_PD14_biological rep 04

description:
WT_PD14_biological rep 04
Gene expression data from the DG of wild-type mouse.

value: Postnatal day 14


title: WT_PD17_biological rep 01

description:
WT_PD17_biological rep 01
Gene expression data from the DG of wild-type mouse.


title: WT_PD17_biological rep 02

description:
WT_PD17_biological rep 02
Gene expression data from the DG of wild-type mouse.


title: WT_PD14_biological rep 05

description:
WT_PD14_biological rep 05
Gene expression data from the DG of wild-type mouse.


title: WT_PD21_biological rep 05

description:
WT_PD21_biological rep 05
Gene expression data from the DG of wild-type mouse.

value: Postnatal day 21


title: WT_PD14_biological rep 03

description:
WT_PD14_biological rep 03
Gene expression data from the DG of wild-type mouse.


title: WT_PD29_biological rep 05

description:
WT_PD29_biological rep 05
Gene expression data from the DG of wild-type mouse.

value: Postnatal day 29


title: WT_PD21_biological rep 03

description:
WT_PD21_biological rep 03
Gene expression data from the DG of wild-type mouse.


title: WT_PD21_biological rep 04

description:
WT_PD21_biological rep 04
Gene expression data from the DG of wild-type mouse.


title: WT_PD21_biological rep 02

description:
WT_PD21_biological rep 02
Gene expression data from the DG of wild-type mouse.


title: Pdx1Cre_E13.5_1_14

city: Beijing

first: Cheng-ran

last: Xu

address: NO.5 YIHEYUAN ROAD HAIDIAN DISTRICT, BEIJING, P.R.CHINA

department: School of Basic Medical Sciences

institute: Peking University

data_processing:
Sequencing reads were mapped to mouse genome (mm10) using TopHat (v2.1.0) with the parameters “-o out_dir -G gtf --transcriptome-index trans_index bowtie2_index input_fastq”.
Quantification of reads mapped to each gene was performed with HTSeq (v0.6.0) with the parameters “htseq-count -f bam -r pos -s no -a 30 input_bam gtf”.
The gene expression levels were calculated as transcripts per million (TPM).
mm10
values tab-delimited text files include read count and TPM values for each sample

source_name: Pancreas

extract_protocol:
The methods of cell lysis and cDNA synthesis (18 cycles for PCR pre-amplification step) followed the Smart-seq2 described by Picelli et al. Then the cDNA was purified with 1X VAHTS DNA Clean Beads (Vazyme. N411-03) twice.
The library was prepared with 2 ng cDNA for 8 cycles PCR amplification using a TruePrep DNA Library Prep Kit (Vazyme, TD502).

tag: cell source

tag: embryonic day


title: Pdx1Cre_E13.5_1_10


title: Pdx1Cre_E13.5_1_16


title: WT_PD29_biological rep 04

description:
WT_PD29_biological rep 04
Gene expression data from the DG of wild-type mouse.


title: Pdx1Cre_E13.5_1_09


title: Pdx1Cre_E13.5_1_12


title: Pdx1Cre_E13.5_1_08


title: Pdx1Cre_E13.5_1_15


title: Pdx1Cre_E13.5_1_04


title: Pdx1Cre_E13.5_1_07


title: Pdx1Cre_E13.5_1_13


title: Pdx1Cre_E13.5_1_06


title: Pdx1Cre_E13.5_1_05


title: WT_PD29_biological rep 03

description:
WT_PD29_biological rep 03
Gene expression data from the DG of wild-type mouse.


title: WT_PD14_biological rep 02

description:
WT_PD14_biological rep 02
Gene expression data from the DG of wild-type mouse.


title: WT_PD29_biological rep 02

description:
WT_PD29_biological rep 02
Gene expression data from the DG of wild-type mouse.


title: WT_PD14_biological rep 01

description:
WT_PD14_biological rep 01
Gene expression data from the DG of wild-type mouse.


title: Pdx1Cre_E13.5_1_03


title: WT_PD29_biological rep 01

description:
WT_PD29_biological rep 01
Gene expression data from the DG of wild-type mouse.


title: Pdx1Cre_E13.5_1_02


title: WT_PD21_biological rep 01

description:
WT_PD21_biological rep 01
Gene expression data from the DG of wild-type mouse.


title: Pdx1Cre_E13.5_1_01


title: Pdx1Cre_E12.5_3_48


title: Pdx1Cre_E12.5_3_47


title: Pdx1Cre_E12.5_3_46


title: Pdx1Cre_E12.5_3_45


title: Pdx1Cre_E12.5_3_44


title: Pdx1Cre_E12.5_3_43


title: Pdx1Cre_E12.5_3_38


title: Pdx1Cre_E12.5_3_37


title: Pdx1Cre_E12.5_3_34


title: Pdx1Cre_E12.5_3_36


title: Pdx1Cre_E12.5_3_42


title: Pdx1Cre_E12.5_3_40


title: Pdx1Cre_E12.5_3_26


title: Pdx1Cre_E12.5_3_27


title: Pdx1Cre_E12.5_3_29


title: Pdx1Cre_E12.5_3_28


title: Pdx1Cre_E12.5_3_31


title: Pdx1Cre_E12.5_3_30


title: Pdx1Cre_E12.5_3_25


title: Pdx1Cre_E12.5_3_41


title: Pdx1Cre_E12.5_3_35


title: Pdx1Cre_E12.5_3_33


title: Pdx1Cre_E12.5_3_39


title: Pdx1Cre_E12.5_3_32


title: Pdx1Cre_E12.5_3_22


title: Pdx1Cre_E12.5_3_20


title: Pdx1Cre_E12.5_3_21


title: Pdx1Cre_E12.5_3_19


title: Pdx1Cre_E12.5_3_23


title: Pdx1Cre_E12.5_3_18


title: Pdx1Cre_E12.5_3_17


title: Pdx1Cre_E12.5_3_15


title: Pdx1Cre_E12.5_3_16


title: Pdx1Cre_E12.5_3_14


title: Pdx1Cre_E12.5_3_13


title: Pdx1Cre_E12.5_3_24


title: Pdx1Cre_E12.5_3_11


title: Pdx1Cre_E12.5_3_09


title: Pdx1Cre_E12.5_3_12


title: Pdx1Cre_E12.5_3_10


title: Pdx1Cre_E12.5_3_08


title: Pdx1Cre_E12.5_3_02


title: Pdx1Cre_E12.5_3_04


title: Pdx1Cre_E12.5_3_03


title: Pdx1Cre_E12.5_3_01


title: Pdx1Cre_E12.5_3_06


title: Pdx1Cre_E12.5_2_07


title: Pdx1Cre_E12.5_2_04


title: Pdx1Cre_E12.5_3_07


title: Pdx1Cre_E12.5_2_06


title: Pdx1Cre_E12.5_3_05


title: Pdx1Cre_E12.5_2_05


title: Pdx1Cre_E12.5_2_03


title: Pdx1Cre_E12.5_1_37


title: Pdx1Cre_E12.5_1_38


title: Pdx1Cre_E12.5_2_02


title: Pdx1Cre_E12.5_1_35


title: Pdx1Cre_E12.5_2_01


title: Pdx1Cre_E12.5_1_39


title: Pdx1Cre_E12.5_1_36


title: Pdx1Cre_E12.5_1_40


title: Pdx1Cre_E12.5_1_33


title: Pdx1Cre_E12.5_1_32


title: Pdx1Cre_E12.5_1_31


title: Pdx1Cre_E12.5_1_28


title: Pdx1Cre_E12.5_1_30


title: Pdx1Cre_E12.5_1_27


title: Pdx1Cre_E12.5_1_29


title: Pdx1Cre_E12.5_1_26


title: Pdx1Cre_E12.5_1_23


title: Pdx1Cre_E12.5_1_25


title: Pdx1Cre_E12.5_1_22


title: Pdx1Cre_E12.5_1_24


title: Pdx1Cre_E12.5_1_15


title: Pdx1Cre_E12.5_1_18


title: Pdx1Cre_E12.5_1_21


title: Pdx1Cre_E12.5_1_13


title: Pdx1Cre_E12.5_1_16


title: Pdx1Cre_E12.5_1_17


title: Pdx1Cre_E12.5_1_19


title: Pdx1Cre_E12.5_1_34


title: Pdx1Cre_E12.5_1_14


title: Pdx1Cre_E12.5_1_10


title: Pdx1Cre_E12.5_1_09


title: Pdx1Cre_E12.5_1_11


title: Pdx1Cre_E12.5_1_06


title: Pdx1Cre_E12.5_1_07


title: Pdx1Cre_E12.5_1_05


title: Pdx1Cre_E12.5_1_04


title: Pdx1Cre_E12.5_1_20


title: Pdx1Cre_E12.5_1_03


title: Pdx1Cre_E12.5_1_01


title: Pdx1Cre_E11.5_2_48


title: Pdx1Cre_E11.5_2_47


title: Pdx1Cre_E11.5_2_46


title: Pdx1Cre_E12.5_1_12


title: Pdx1Cre_E11.5_2_44


title: Pdx1Cre_E11.5_2_42


title: Pdx1Cre_E11.5_2_39


title: Pdx1Cre_E11.5_2_41


title: Pdx1Cre_E11.5_2_38


title: Pdx1Cre_E11.5_2_37


title: Pdx1Cre_E11.5_2_36


title: Pdx1Cre_E12.5_1_08


title: Pdx1Cre_E11.5_2_34


title: Pdx1Cre_E11.5_2_33


title: Pdx1Cre_E11.5_2_32


title: Pdx1Cre_E11.5_2_30


title: Pdx1Cre_E11.5_2_29


title: Pdx1Cre_E11.5_2_28


title: Pdx1Cre_E12.5_1_02


title: Pdx1Cre_E11.5_2_26


title: Pdx1Cre_E11.5_2_45


title: Pdx1Cre_E11.5_2_21


title: Pdx1Cre_E11.5_2_20


title: Pdx1Cre_E11.5_2_23


title: Pdx1Cre_E11.5_2_22


title: Pdx1Cre_E11.5_2_24


title: Pdx1Cre_E11.5_2_25


title: Pdx1Cre_E11.5_2_35


title: Pdx1Cre_E11.5_2_43


title: Pdx1Cre_E11.5_2_15


title: Pdx1Cre_E11.5_2_16


title: Pdx1Cre_E11.5_2_40


title: Pdx1Cre_E11.5_2_17


title: Pdx1Cre_E11.5_2_19


title: Pdx1Cre_E11.5_2_13


title: Pdx1Cre_E11.5_2_11


title: Pdx1Cre_E11.5_2_18


title: Pdx1Cre_E11.5_2_14


title: Pdx1Cre_E11.5_2_09


title: Pdx1Cre_E11.5_2_27


title: Pdx1Cre_E11.5_2_10


title: Pdx1Cre_E11.5_2_07


title: Pdx1Cre_E11.5_2_08


title: Pdx1Cre_E11.5_2_12


title: Pdx1Cre_E11.5_2_06


title: Pdx1Cre_E11.5_2_05


title: Pdx1Cre_E13.5_1_11


title: Pdx1Cre_E11.5_2_31


title: Pdx1Cre_E11.5_2_04


title: Pdx1Cre_E11.5_2_03


title: Pdx1Cre_E11.5_2_02


title: Pdx1Cre_E11.5_1_31


title: Pdx1Cre_E11.5_1_30


title: Pdx1Cre_E11.5_1_32


title: Pdx1Cre_E11.5_2_01


title: Pdx1Cre_E11.5_1_28


title: Pdx1Cre_E11.5_1_29


title: Pdx1Cre_E11.5_1_26


title: Pdx1Cre_E11.5_1_27


title: Pdx1Cre_E11.5_1_23


title: Pdx1Cre_E11.5_1_25


title: Pdx1Cre_E11.5_1_21


title: Pdx1Cre_E11.5_1_19


title: Pdx1Cre_E11.5_1_20


title: Pdx1Cre_E11.5_1_16


title: Pdx1Cre_E11.5_1_12


title: Pdx1Cre_E11.5_1_14


title: Pdx1Cre_E11.5_1_17


title: Pdx1Cre_E11.5_1_15


title: Pdx1Cre_E11.5_1_09


title: Pdx1Cre_E11.5_1_07


title: Pdx1Cre_E11.5_1_18


title: Pdx1Cre_E11.5_1_08


title: Pdx1Cre_E11.5_1_13


title: Pdx1Cre_E11.5_1_11


title: Pdx1Cre_E11.5_1_10


title: Pdx1Cre_E11.5_1_05


title: Pdx1Cre_E11.5_1_04


title: Pdx1Cre_E11.5_1_06


title: Pdx1Cre_E11.5_1_22


title: Pdx1Cre_E11.5_1_03


title: Pdx1Cre_E11.5_1_01


title: Pdx1Cre_E10.5_2_24


title: Pdx1Cre_E11.5_1_02


title: Pdx1Cre_E10.5_2_23


title: Pdx1Cre_E11.5_1_24


title: Pdx1Cre_E10.5_2_20


title: Pdx1Cre_E10.5_2_21


title: Pdx1Cre_E10.5_2_22


title: Pdx1Cre_E10.5_2_18


title: Pdx1Cre_E10.5_2_17


title: Pdx1Cre_E10.5_2_16


title: Pdx1Cre_E10.5_2_19


title: Pdx1Cre_E10.5_2_14


title: Pdx1Cre_E10.5_2_10


title: Pdx1Cre_E10.5_2_08


title: Pdx1Cre_E10.5_2_09


title: Pdx1Cre_E10.5_2_13


title: Pdx1Cre_E10.5_2_07


title: Pdx1Cre_E10.5_2_06


title: Pdx1Cre_E10.5_2_05


title: Pdx1Cre_E10.5_2_12


title: Pdx1Cre_E10.5_2_02


title: Pdx1Cre_E10.5_2_01


title: Pdx1Cre_E10.5_1_56


title: Pdx1Cre_E10.5_2_03


title: Pdx1Cre_E10.5_1_55


title: Pdx1Cre_E10.5_1_52


title: Pdx1Cre_E10.5_1_51


title: Pdx1Cre_E10.5_2_15


title: Pdx1Cre_E10.5_1_48


title: Pdx1Cre_E10.5_1_47


title: Pdx1Cre_E10.5_1_49


title: Pdx1Cre_E10.5_1_50


title: Pdx1Cre_E10.5_1_42


title: Pdx1Cre_E10.5_1_46


title: Pdx1Cre_E10.5_1_45


title: Pdx1Cre_E10.5_1_44


title: Pdx1Cre_E10.5_2_04


title: Pdx1Cre_E10.5_1_41


title: Pdx1Cre_E10.5_1_43


title: Pdx1Cre_E10.5_1_36


title: Pdx1Cre_E10.5_1_33


title: Pdx1Cre_E10.5_1_32


title: Pdx1Cre_E10.5_2_11


title: Pdx1Cre_E10.5_1_31


title: Pdx1Cre_E10.5_1_40


title: Pdx1Cre_E10.5_1_39


title: Pdx1Cre_E10.5_1_54


title: Pdx1Cre_E10.5_1_53


title: Pdx1Cre_E10.5_1_30


title: Pdx1Cre_E10.5_1_28


title: Pdx1Cre_E10.5_1_29


title: Pdx1Cre_E10.5_1_25


title: Pdx1Cre_E10.5_1_24


title: Pdx1Cre_E10.5_1_23


title: Pdx1Cre_E10.5_1_26


title: Pdx1Cre_E10.5_1_22


title: Pdx1Cre_E10.5_1_21


title: Pdx1Cre_E10.5_1_38


title: Pdx1Cre_E10.5_1_19


title: Pdx1Cre_E10.5_1_18


title: Pdx1Cre_E10.5_1_35


title: Pdx1Cre_E10.5_1_37


title: Pdx1Cre_E10.5_1_17


title: Pdx1Cre_E10.5_1_20


title: Pdx1Cre_E10.5_1_14


title: Pdx1Cre_E10.5_1_34


title: Pdx1Cre_E10.5_1_16


title: Pdx1Cre_E10.5_1_12


title: Pdx1Cre_E10.5_1_13


title: Pdx1Cre_E10.5_1_10


title: Pdx1Cre_E10.5_1_08


title: Pdx1Cre_E10.5_1_06


title: Pdx1Cre_E10.5_1_07


title: Pdx1Cre_E10.5_1_09


title: Pdx1Cre_E10.5_1_27


title: Pdx1Cre_E10.5_1_05


title: Pdx1Cre_E10.5_1_04


title: Pdx1Cre_E10.5_1_03


title: Pdx1Cre_E10.5_1_02


title: Ngn3Pos_E17.5_4_29


title: Ngn3Pos_E17.5_4_28


title: Pdx1Cre_E10.5_1_01


title: Ngn3Pos_E17.5_4_26


title: Ngn3Pos_E17.5_4_25


title: Ngn3Pos_E17.5_4_24


title: Ngn3Pos_E17.5_4_23


title: Pdx1Cre_E10.5_1_15


title: Ngn3Pos_E17.5_4_21


title: Ngn3Pos_E17.5_4_19


title: Ngn3Pos_E17.5_4_20


title: Pdx1Cre_E10.5_1_11


title: Ngn3Pos_E17.5_4_18


title: Ngn3Pos_E17.5_4_16


title: Ngn3Pos_E17.5_4_14


title: Ngn3Pos_E17.5_4_15


title: Ngn3Pos_E17.5_4_17


title: Ngn3Pos_E17.5_4_08


title: Ngn3Pos_E17.5_4_07


title: Ngn3Pos_E17.5_4_06


title: Ngn3Pos_E17.5_4_09


title: Ngn3Pos_E17.5_4_12


title: Ngn3Pos_E17.5_4_04


title: Ngn3Pos_E17.5_4_11


title: Ngn3Pos_E17.5_4_05


title: Ngn3Pos_E17.5_4_10


title: Ngn3Pos_E17.5_4_27


title: Ngn3Pos_E17.5_4_03


title: Ngn3Pos_E17.5_4_02


title: Ngn3Pos_E17.5_4_01


title: Ngn3Pos_E17.5_3_22


title: Ngn3Pos_E17.5_3_11


title: Ngn3Pos_E17.5_3_14


title: Ngn3Pos_E17.5_3_16


title: Ngn3Pos_E17.5_4_22


title: Ngn3Pos_E17.5_3_21


title: Ngn3Pos_E17.5_3_07


title: Ngn3Pos_E17.5_3_18


title: Ngn3Pos_E17.5_3_03


title: Ngn3Pos_E17.5_3_15


title: Ngn3Pos_E17.5_3_09


title: Ngn3Pos_E17.5_3_10


title: Ngn3Pos_E17.5_3_06


title: Ngn3Pos_E17.5_3_19


title: Ngn3Pos_E17.5_3_20


title: Ngn3Pos_E17.5_3_12


title: Ngn3Pos_E17.5_3_01


title: Ngn3Pos_E17.5_3_17


title: Ngn3Pos_E17.5_3_13


title: Ngn3Pos_E17.5_2_40


title: Ngn3Pos_E17.5_3_04


title: Ngn3Pos_E17.5_3_05


title: Ngn3Pos_E17.5_3_08


title: Ngn3Pos_E17.5_2_39


title: Ngn3Pos_E17.5_3_02


title: Ngn3Pos_E17.5_2_38


title: Ngn3Pos_E17.5_2_36


title: Ngn3Pos_E17.5_4_13


title: Ngn3Pos_E17.5_2_35


title: Ngn3Pos_E17.5_2_34


title: Ngn3Pos_E17.5_2_33


title: Ngn3Pos_E17.5_2_31


title: Ngn3Pos_E17.5_2_30


title: Ngn3Pos_E17.5_2_32


title: Ngn3Pos_E17.5_2_28


title: Ngn3Pos_E17.5_2_29


title: Ngn3Pos_E17.5_2_27


title: Ngn3Pos_E17.5_2_26


title: Ngn3Pos_E17.5_2_25


title: Ngn3Pos_E17.5_2_37


title: Ngn3Pos_E17.5_2_22


title: Ngn3Pos_E17.5_2_15


title: Ngn3Pos_E17.5_2_18


title: Ngn3Pos_E17.5_2_14


title: Ngn3Pos_E17.5_2_21


title: Ngn3Pos_E17.5_2_20


title: Ngn3Pos_E17.5_2_12


title: Ngn3Pos_E17.5_2_19


title: Ngn3Pos_E17.5_2_24


title: Ngn3Pos_E17.5_2_13


title: Ngn3Pos_E17.5_2_17


title: Ngn3Pos_E17.5_2_23


title: Ngn3Pos_E17.5_2_16


title: Ngn3Pos_E17.5_2_08


title: Ngn3Pos_E17.5_2_07


title: Ngn3Pos_E17.5_2_10


title: Ngn3Pos_E17.5_2_11


title: Ngn3Pos_E17.5_2_09


title: Ngn3Pos_E17.5_2_06


title: Ngn3Pos_E17.5_2_04


title: Ngn3Pos_E17.5_2_05


title: Ngn3Pos_E17.5_1_34


title: Ngn3Pos_E17.5_2_02


title: Ngn3Pos_E17.5_2_03


title: Ngn3Pos_E17.5_2_01


title: Ngn3Pos_E17.5_1_33


title: Ngn3Pos_E17.5_1_31


title: Ngn3Pos_E17.5_1_30


title: Ngn3Pos_E17.5_1_29


title: Ngn3Pos_E17.5_1_24


title: Ngn3Pos_E17.5_1_23


title: Ngn3Pos_E17.5_1_25


title: Ngn3Pos_E17.5_1_27


title: Ngn3Pos_E17.5_1_26


title: Ngn3Pos_E17.5_1_21


title: Ngn3Pos_E17.5_1_28


title: Ngn3Pos_E17.5_1_19


title: Ngn3Pos_E17.5_1_16


title: Ngn3Pos_E17.5_1_17


title: Ngn3Pos_E17.5_1_22


title: Ngn3Pos_E17.5_1_13


title: Ngn3Pos_E17.5_1_12


title: Ngn3Pos_E17.5_1_18


title: Ngn3Pos_E17.5_1_14


title: Ngn3Pos_E17.5_1_10


title: Ngn3Pos_E17.5_1_20


title: Ngn3Pos_E17.5_1_15


title: Ngn3Pos_E17.5_1_09


title: Ngn3Pos_E17.5_1_11


title: Ngn3Pos_E17.5_1_08


title: Ngn3Pos_E17.5_1_07


title: Ngn3Pos_E17.5_1_32


title: Ngn3Pos_E17.5_1_06


title: Ngn3Pos_E17.5_1_05


title: Ngn3Pos_E17.5_1_03


title: Ngn3Pos_E17.5_1_04


title: Ngn3Pos_E17.5_1_02


title: Ngn3Pos_E17.5_1_01


title: Ngn3Pos_E16.5_4_35


title: Ngn3Pos_E16.5_4_34


title: Ngn3Pos_E16.5_4_32


title: Ngn3Pos_E16.5_4_33


title: Ngn3Pos_E16.5_4_26


title: Ngn3Pos_E16.5_4_28


title: Ngn3Pos_E16.5_4_24


title: Ngn3Pos_E16.5_4_31


title: Ngn3Pos_E16.5_4_30


title: Ngn3Pos_E16.5_4_27


title: Ngn3Pos_E16.5_4_23


title: Ngn3Pos_E16.5_4_29


title: Ngn3Pos_E16.5_4_22


title: Ngn3Pos_E16.5_4_21


title: Ngn3Pos_E16.5_4_19


title: Ngn3Pos_E16.5_4_17


title: Ngn3Pos_E16.5_4_15


title: Ngn3Pos_E16.5_4_14


title: Ngn3Pos_E16.5_4_20


title: Ngn3Pos_E16.5_4_18


title: Ngn3Pos_E16.5_4_11


title: Ngn3Pos_E16.5_4_12


title: Ngn3Pos_E16.5_4_09


title: Ngn3Pos_E16.5_4_07


title: Ngn3Pos_E16.5_4_16


title: Ngn3Pos_E16.5_4_05


title: Ngn3Pos_E16.5_4_10


title: Ngn3Pos_E16.5_4_08


title: Ngn3Pos_E16.5_4_25


title: Ngn3Pos_E16.5_4_04


title: Ngn3Pos_E16.5_3_41


title: Ngn3Pos_E16.5_4_03


title: Ngn3Pos_E16.5_4_01


title: Ngn3Pos_E16.5_4_13


title: Ngn3Pos_E16.5_3_33


title: Ngn3Pos_E16.5_3_34


title: Ngn3Pos_E16.5_3_35


title: Ngn3Pos_E16.5_3_37


title: Ngn3Pos_E16.5_3_30


title: Ngn3Pos_E16.5_3_36


title: Ngn3Pos_E16.5_3_28


title: Ngn3Pos_E16.5_3_38


title: Ngn3Pos_E16.5_3_27


title: Ngn3Pos_E16.5_3_25


title: Ngn3Pos_E16.5_3_29


title: Ngn3Pos_E16.5_3_24


title: Ngn3Pos_E16.5_3_22


title: Ngn3Pos_E16.5_3_26


title: Ngn3Pos_E16.5_4_06


title: Ngn3Pos_E16.5_3_23


title: Ngn3Pos_E16.5_3_20


title: Ngn3Pos_E16.5_3_21


title: Ngn3Pos_E16.5_3_19


title: Ngn3Pos_E16.5_3_18


title: Ngn3Pos_E16.5_4_02


title: Ngn3Pos_E16.5_3_17


title: Ngn3Pos_E16.5_3_40


title: Ngn3Pos_E16.5_3_39


title: Ngn3Pos_E16.5_3_14


title: Ngn3Pos_E16.5_3_16


title: Ngn3Pos_E16.5_3_31


title: Ngn3Pos_E16.5_3_32


title: Ngn3Pos_E16.5_3_13


title: Ngn3Pos_E16.5_3_11


title: Ngn3Pos_E16.5_3_12


title: Ngn3Pos_E16.5_3_06


title: Ngn3Pos_E16.5_3_07


title: Ngn3Pos_E16.5_3_08


title: Ngn3Pos_E16.5_3_09


title: Ngn3Pos_E16.5_3_04


title: Ngn3Pos_E16.5_2_17


title: Ngn3Pos_E16.5_3_02


title: Ngn3Pos_E16.5_2_15


title: Ngn3Pos_E16.5_2_16


title: Ngn3Pos_E16.5_3_03


title: Ngn3Pos_E16.5_2_12


title: Ngn3Pos_E16.5_3_01


title: Ngn3Pos_E16.5_2_13


title: Ngn3Pos_E16.5_2_14


title: Ngn3Pos_E16.5_2_09


title: Ngn3Pos_E16.5_2_07


title: Ngn3Pos_E16.5_2_10


title: Ngn3Pos_E16.5_3_15


title: Ngn3Pos_E16.5_2_08


title: Ngn3Pos_E16.5_2_11


title: Ngn3Pos_E16.5_2_06


title: Ngn3Pos_E16.5_2_02


title: Ngn3Pos_E16.5_2_03


title: Ngn3Pos_E16.5_2_05


title: Ngn3Pos_E16.5_3_10


title: Ngn3Pos_E16.5_2_01


title: Ngn3Pos_E16.5_2_04


title: Ngn3Pos_E16.5_3_05


title: Ngn3Pos_E16.5_1_36


title: Ngn3Pos_E16.5_1_37


title: Ngn3Pos_E16.5_1_33


title: Ngn3Pos_E16.5_1_35


title: Ngn3Pos_E16.5_1_32


title: Ngn3Pos_E16.5_1_31


title: Ngn3Pos_E16.5_1_29


title: Ngn3Pos_E16.5_1_28


title: Ngn3Pos_E16.5_1_30


title: Ngn3Pos_E16.5_1_25


title: Ngn3Pos_E16.5_1_23


title: Ngn3Pos_E16.5_1_27


title: Ngn3Pos_E16.5_1_26


title: Ngn3Pos_E16.5_1_22


title: Ngn3Pos_E16.5_1_18


title: Ngn3Pos_E16.5_1_20


title: Ngn3Pos_E16.5_1_17


title: Ngn3Pos_E16.5_1_16


title: Ngn3Pos_E16.5_1_21


title: Ngn3Pos_E16.5_1_19


title: Ngn3Pos_E16.5_1_15


title: Ngn3Pos_E16.5_1_12


title: Ngn3Pos_E16.5_1_11


title: Ngn3Pos_E16.5_1_09


title: Ngn3Pos_E16.5_1_10


title: Ngn3Pos_E16.5_1_34


title: Ngn3Pos_E16.5_1_14


title: Ngn3Pos_E16.5_1_13


title: Ngn3Pos_E16.5_1_08


title: Ngn3Pos_E16.5_1_07


title: Ngn3Pos_E16.5_1_05


title: Ngn3Pos_E16.5_1_04


title: Ngn3Pos_E16.5_1_06


title: Ngn3Pos_E16.5_1_03


title: Ngn3Pos_E16.5_1_24


title: Ngn3Pos_E16.5_1_02


title: Ngn3Pos_E12.5_3_38


title: Ngn3Pos_E12.5_3_37


title: Ngn3Pos_E12.5_3_35


title: Ngn3Pos_E12.5_3_36


title: Ngn3Pos_E12.5_3_34


title: Ngn3Pos_E12.5_3_33


title: Ngn3Pos_E12.5_3_31


title: Ngn3Pos_E12.5_3_30


title: Ngn3Pos_E12.5_3_29


title: Ngn3Pos_E12.5_3_26


title: Ngn3Pos_E12.5_3_27


title: Ngn3Pos_E12.5_3_25


title: Ngn3Pos_E12.5_3_28


title: Ngn3Pos_E12.5_3_24


title: Ngn3Pos_E12.5_3_23


title: Ngn3Pos_E12.5_3_21


title: Ngn3Pos_E12.5_3_19


title: Ngn3Pos_E12.5_3_20


title: Ngn3Pos_E12.5_3_22


title: Ngn3Pos_E12.5_3_16


title: Ngn3Pos_E12.5_3_14


title: Ngn3Pos_E12.5_3_11


title: Ngn3Pos_E12.5_3_15


title: Ngn3Pos_E16.5_1_01


title: Ngn3Pos_E12.5_3_17


title: Ngn3Pos_E12.5_3_18


title: Ngn3Pos_E12.5_3_06


title: Ngn3Pos_E12.5_3_09


title: Ngn3Pos_E12.5_3_32


title: Ngn3Pos_E12.5_3_04


title: Ngn3Pos_E12.5_3_05


title: Ngn3Pos_E12.5_3_03


title: Ngn3Pos_E12.5_3_02


title: Ngn3Pos_E12.5_2_36


title: Ngn3Pos_E12.5_2_35


title: Ngn3Pos_E12.5_2_32


title: Ngn3Pos_E12.5_2_31


title: Ngn3Pos_E12.5_2_30


title: Ngn3Pos_E12.5_2_34


title: Ngn3Pos_E12.5_2_29


title: Ngn3Pos_E12.5_3_13


title: Ngn3Pos_E12.5_3_12


title: Ngn3Pos_E12.5_3_07


title: Ngn3Pos_E12.5_3_10


title: Ngn3Pos_E12.5_2_26


title: Ngn3Pos_E12.5_2_25


title: Ngn3Pos_E12.5_2_28


title: Ngn3Pos_E12.5_2_24


title: Ngn3Pos_E12.5_3_08


title: Ngn3Pos_E12.5_2_23


title: Ngn3Pos_E12.5_2_21


title: Ngn3Pos_E12.5_2_17


title: Ngn3Pos_E12.5_2_19


title: Ngn3Pos_E12.5_2_20


title: Ngn3Pos_E12.5_2_22


title: Ngn3Pos_E12.5_3_01


title: Ngn3Pos_E12.5_2_18


title: Ngn3Pos_E12.5_2_15


title: Ngn3Pos_E12.5_2_16


title: Ngn3Pos_E12.5_2_14


title: Ngn3Pos_E12.5_2_33


title: Ngn3Pos_E12.5_2_12


title: Ngn3Pos_E12.5_2_27


title: Ngn3Pos_E12.5_2_13


title: Ngn3Pos_E12.5_2_09


title: Ngn3Pos_E12.5_2_08


title: Ngn3Pos_E12.5_2_11


title: Ngn3Pos_E12.5_2_06


title: Ngn3Pos_E12.5_2_05


title: Ngn3Pos_E12.5_2_04


title: Ngn3Pos_E12.5_2_07


title: Ngn3Pos_E12.5_2_03


title: Ngn3Pos_E12.5_1_47


title: Ngn3Pos_E12.5_1_46


title: Ngn3Pos_E12.5_1_45


title: Ngn3Pos_E12.5_2_02


title: Ngn3Pos_E12.5_1_44


title: Ngn3Pos_E12.5_1_42


title: Ngn3Pos_E12.5_1_39


title: Ngn3Pos_E12.5_1_38


title: Ngn3Pos_E12.5_1_37


title: Ngn3Pos_E12.5_1_36


title: Ngn3Pos_E12.5_1_40


title: Ngn3Pos_E12.5_1_41


title: Ngn3Pos_E12.5_2_10


title: Ngn3Pos_E12.5_1_34


title: Ngn3Pos_E12.5_1_33


title: Ngn3Pos_E12.5_1_32


title: Ngn3Pos_E12.5_1_30


title: Ngn3Pos_E12.5_1_28


title: Ngn3Pos_E12.5_1_31


title: Ngn3Pos_E12.5_1_27


title: Ngn3Pos_E12.5_1_29


title: Ngn3Pos_E12.5_1_26


title: Ngn3Pos_E12.5_2_01


title: Ngn3Pos_E12.5_1_25


title: Ngn3Pos_E12.5_1_23


title: Ngn3Pos_E12.5_1_21


title: Ngn3Pos_E12.5_1_24


title: Ngn3Pos_E12.5_1_22


title: Ngn3Pos_E12.5_1_19


title: Ngn3Pos_E12.5_1_18


title: Ngn3Pos_E12.5_1_17


title: Ngn3Pos_E12.5_1_43


title: Ngn3Pos_E12.5_1_16


title: Ngn3Pos_E12.5_1_15


title: Ngn3Pos_E12.5_1_35


title: Ngn3Pos_E12.5_1_09


title: Ngn3Pos_E12.5_1_13


title: Ngn3Pos_E12.5_1_14


title: Ngn3Pos_E12.5_1_12


title: Ngn3Pos_E12.5_1_11


title: Ngn3Pos_E12.5_1_07


title: Ngn3Pos_E12.5_1_08


title: Ngn3Pos_E12.5_1_06


title: Ngn3Pos_E12.5_1_05


title: Ngn3Pos_E12.5_1_04


title: Ngn3Pos_E10.5_3_24


title: Ngn3Pos_E12.5_1_01


title: Ngn3Pos_E12.5_1_02


title: Ngn3Pos_E10.5_3_23


title: Ngn3Pos_E12.5_1_03


title: Ngn3Pos_E12.5_1_20


title: Ngn3Pos_E10.5_3_21


title: Ngn3Pos_E10.5_3_19


title: Ngn3Pos_E10.5_3_18


title: Ngn3Pos_E10.5_3_17


title: Ngn3Pos_E10.5_3_20


title: Ngn3Pos_E10.5_3_15


title: Ngn3Pos_E10.5_3_22


title: Ngn3Pos_E10.5_3_13


title: Ngn3Pos_E12.5_1_10


title: Ngn3Pos_E10.5_3_12


title: Ngn3Pos_E10.5_3_14


title: Ngn3Pos_E10.5_3_09


title: Ngn3Pos_E10.5_3_07


title: Ngn3Pos_E10.5_3_16


title: Ngn3Pos_E10.5_3_11


title: Ngn3Pos_E10.5_3_10


title: Ngn3Pos_E10.5_3_06


title: Ngn3Pos_E10.5_3_05


title: Ngn3Pos_E10.5_3_08


title: Ngn3Pos_E10.5_3_02


title: Ngn3Pos_E10.5_3_03


title: Ngn3Pos_E10.5_3_01


title: Ngn3Pos_E10.5_2_17


title: Ngn3Pos_E10.5_2_16


title: Ngn3Pos_E10.5_2_15


title: Ngn3Pos_E10.5_2_14


title: Ngn3Pos_E10.5_2_13


title: Ngn3Pos_E10.5_3_04


title: Ngn3Pos_E10.5_2_12


title: Ngn3Pos_E10.5_2_10


title: Ngn3Pos_E10.5_2_09


title: Ngn3Pos_E10.5_2_08


title: Ngn3Pos_E10.5_2_07


title: Ngn3Pos_E10.5_2_11


title: U0126_Ins1Pos_E13.0_CultureFor2Days_4_13

growth_protocol: E13.0 dorsal pancreata were dissected from Ins1-RFP transgenic mice. Three pancreata were cultured on one Whatman® Nuclepore Track-Etched Membranes (110414) in 12-well plate with 2ml DMEM media (Gibco, 11885), containing 10% FBS and 1% penicillin/streptomycin. The tissues were cultured at 37°C and 5% CO2 in a humidified incubator for 48h. Then the tissues were harvested and digested with 0.5 mg/mL of collagenase P for 1 min at 37°C, then followed by 0.25% trypsin for 4 min at 37°C.

treatment_protocol: U0126 (Sigma, U120), inhibitor of MEK1 and MEK2, was added to the media to final concentration of 10 µM.

value: Pancreas

tag: cultured for

value: 2 days

tag: treatment


title: U0126_Ins1Pos_E13.0_CultureFor2Days_4_09


title: U0126_Ins1Pos_E13.0_CultureFor2Days_4_19


title: U0126_Ins1Pos_E13.0_CultureFor2Days_4_16


title: U0126_Ins1Pos_E13.0_CultureFor2Days_4_17


title: U0126_Ins1Pos_E13.0_CultureFor2Days_4_15


title: U0126_Ins1Pos_E13.0_CultureFor2Days_4_20


title: U0126_Ins1Pos_E13.0_CultureFor2Days_4_14


title: U0126_Ins1Pos_E13.0_CultureFor2Days_4_12


title: U0126_Ins1Pos_E13.0_CultureFor2Days_4_18


title: U0126_Ins1Pos_E13.0_CultureFor2Days_4_08


title: U0126_Ins1Pos_E13.0_CultureFor2Days_4_11


title: U0126_Ins1Pos_E13.0_CultureFor2Days_4_10


title: U0126_Ins1Pos_E13.0_CultureFor2Days_4_07


title: U0126_Ins1Pos_E13.0_CultureFor2Days_4_01


title: U0126_Ins1Pos_E13.0_CultureFor2Days_4_04


title: U0126_Ins1Pos_E13.0_CultureFor2Days_4_03


title: Control_Ins1Pos_E13.0_CultureFor2Days_4_18

value: Control


title: U0126_Ins1Pos_E13.0_CultureFor2Days_4_05


title: U0126_Ins1Pos_E13.0_CultureFor2Days_4_06


title: Control_Ins1Pos_E13.0_CultureFor2Days_4_19


title: Control_Ins1Pos_E13.0_CultureFor2Days_4_20


title: U0126_Ins1Pos_E13.0_CultureFor2Days_4_02


title: Control_Ins1Pos_E13.0_CultureFor2Days_4_13


title: Control_Ins1Pos_E13.0_CultureFor2Days_4_14


title: Control_Ins1Pos_E13.0_CultureFor2Days_4_12


title: Control_Ins1Pos_E13.0_CultureFor2Days_4_11


title: Control_Ins1Pos_E13.0_CultureFor2Days_4_15


title: Control_Ins1Pos_E13.0_CultureFor2Days_4_16


title: Control_Ins1Pos_E13.0_CultureFor2Days_4_10


title: Control_Ins1Pos_E13.0_CultureFor2Days_4_08


title: Control_Ins1Pos_E13.0_CultureFor2Days_4_07


title: Control_Ins1Pos_E13.0_CultureFor2Days_4_17


title: Control_Ins1Pos_E13.0_CultureFor2Days_4_02


title: Control_Ins1Pos_E13.0_CultureFor2Days_4_03


title: Control_Ins1Pos_E13.0_CultureFor2Days_4_05


title: Control_Ins1Pos_E13.0_CultureFor2Days_4_09


title: Ptf1aCreER_E11.5_3_18


title: Control_Ins1Pos_E13.0_CultureFor2Days_4_01


title: Control_Ins1Pos_E13.0_CultureFor2Days_4_06


title: Ptf1aCreER_E11.5_3_17


title: Ptf1aCreER_E11.5_3_15


title: Ptf1aCreER_E11.5_3_14


title: Ptf1aCreER_E11.5_3_16


title: Ptf1aCreER_E11.5_3_12


title: Ptf1aCreER_E11.5_3_11


title: Ptf1aCreER_E11.5_3_09


title: Ptf1aCreER_E11.5_3_04


title: Control_Ins1Pos_E13.0_CultureFor2Days_4_04


title: Ptf1aCreER_E11.5_3_13


title: Ptf1aCreER_E11.5_3_10


title: Ptf1aCreER_E11.5_3_05


title: Ptf1aCreER_E11.5_3_06


title: Ptf1aCreER_E11.5_3_03


title: Ptf1aCreER_E11.5_3_01


title: Ptf1aCreER_E11.5_3_02


title: Ptf1aCreER_E11.5_2_05


title: Ptf1aCreER_E11.5_2_03


title: Ptf1aCreER_E11.5_2_04


title: Ptf1aCreER_E11.5_2_06


title: Ptf1aCreER_E11.5_1_54


title: Ptf1aCreER_E11.5_2_01


title: Ptf1aCreER_E11.5_2_02


title: Ptf1aCreER_E11.5_1_51


title: Ptf1aCreER_E11.5_1_53


title: Ptf1aCreER_E11.5_1_50


title: Ptf1aCreER_E11.5_3_07


title: Ptf1aCreER_E11.5_1_49


title: Ptf1aCreER_E11.5_1_55


title: Ptf1aCreER_E11.5_1_52


title: Ptf1aCreER_E11.5_3_08


title: Ptf1aCreER_E11.5_1_45


title: Ptf1aCreER_E11.5_1_43


title: Ptf1aCreER_E11.5_1_46


title: Ptf1aCreER_E11.5_1_42


title: Ptf1aCreER_E11.5_1_40


title: Ptf1aCreER_E11.5_1_41


title: Ptf1aCreER_E11.5_1_39


title: Ptf1aCreER_E11.5_1_38


title: Ptf1aCreER_E11.5_1_37


title: Ptf1aCreER_E11.5_1_34


title: Ptf1aCreER_E11.5_1_32


title: Ptf1aCreER_E11.5_1_33


title: Ptf1aCreER_E11.5_1_48


title: Ptf1aCreER_E11.5_1_30


title: Ptf1aCreER_E11.5_1_47


title: Ptf1aCreER_E11.5_1_31


title: Ptf1aCreER_E11.5_1_29


title: Ptf1aCreER_E11.5_1_27


title: Ptf1aCreER_E11.5_1_26


title: Ptf1aCreER_E11.5_1_44


title: Ptf1aCreER_E11.5_1_24


title: Ptf1aCreER_E11.5_1_20


title: Ptf1aCreER_E11.5_1_23


title: Ptf1aCreER_E11.5_1_22


title: Ptf1aCreER_E11.5_1_19


title: Ptf1aCreER_E11.5_1_25


title: Ptf1aCreER_E11.5_1_21


title: Ptf1aCreER_E11.5_1_17


title: Ptf1aCreER_E11.5_1_36


title: Ptf1aCreER_E11.5_1_18


title: Ptf1aCreER_E11.5_1_35


title: Ptf1aCreER_E11.5_1_16


title: Ptf1aCreER_E11.5_1_15


title: Ptf1aCreER_E11.5_1_13


title: Ptf1aCreER_E11.5_1_14


title: Ptf1aCreER_E11.5_1_09


title: Ptf1aCreER_E11.5_1_10


title: Ptf1aCreER_E11.5_1_11


title: Ptf1aCreER_E11.5_1_07


title: Ptf1aCreER_E11.5_1_08


title: Ptf1aCreER_E11.5_1_28


title: Ptf1aCreER_E11.5_1_06


title: Ptf1aCreER_E11.5_1_03


title: Ptf1aCreER_E11.5_1_04


title: Ptf1aCreER_E11.5_1_02


title: Pdx1Cre_E14.5_4_44


title: Ptf1aCreER_E11.5_1_01


title: Pdx1Cre_E14.5_4_43


title: Pdx1Cre_E14.5_4_41


title: Pdx1Cre_E14.5_4_40


title: Pdx1Cre_E14.5_4_39


title: Pdx1Cre_E14.5_4_42


title: Pdx1Cre_E14.5_4_36


title: Pdx1Cre_E14.5_4_34


title: Pdx1Cre_E14.5_4_33


title: Pdx1Cre_E14.5_4_38


title: Pdx1Cre_E14.5_4_37


title: Ptf1aCreER_E11.5_1_12


title: Pdx1Cre_E14.5_4_35


title: Pdx1Cre_E14.5_4_29


title: Pdx1Cre_E14.5_4_28


title: Pdx1Cre_E14.5_4_30


title: Pdx1Cre_E14.5_4_26


title: Pdx1Cre_E14.5_4_27


title: Pdx1Cre_E14.5_4_32


title: Pdx1Cre_E14.5_4_25


title: Ptf1aCreER_E11.5_1_05


title: Pdx1Cre_E14.5_4_24


title: Pdx1Cre_E14.5_4_23


title: Pdx1Cre_E14.5_4_19


title: Pdx1Cre_E14.5_4_21


title: Pdx1Cre_E14.5_4_16


title: Pdx1Cre_E14.5_4_18


title: Pdx1Cre_E14.5_4_14


title: Pdx1Cre_E14.5_4_22


title: Pdx1Cre_E14.5_4_10


title: Pdx1Cre_E14.5_4_17


title: Pdx1Cre_E14.5_4_09


title: Pdx1Cre_E14.5_4_08


title: Pdx1Cre_E14.5_4_13


title: Pdx1Cre_E14.5_4_11


title: Pdx1Cre_E14.5_4_31


title: Pdx1Cre_E14.5_4_15


title: Pdx1Cre_E14.5_4_05


title: Pdx1Cre_E14.5_4_07


title: Pdx1Cre_E14.5_4_06


title: Pdx1Cre_E14.5_4_01


title: Pdx1Cre_E12.5_4_33


title: Pdx1Cre_E14.5_4_20


title: Pdx1Cre_E14.5_4_02


title: Pdx1Cre_E12.5_4_32


title: Pdx1Cre_E14.5_4_04


title: Pdx1Cre_E12.5_4_36


title: Pdx1Cre_E14.5_4_03


title: Pdx1Cre_E12.5_4_29


title: Pdx1Cre_E12.5_4_35


title: Pdx1Cre_E14.5_4_12


title: Pdx1Cre_E12.5_4_30


title: Pdx1Cre_E12.5_4_34


title: Pdx1Cre_E12.5_4_27


title: Pdx1Cre_E12.5_4_28


title: Pdx1Cre_E12.5_4_24


title: Pdx1Cre_E12.5_4_26


title: Pdx1Cre_E12.5_4_22


title: Pdx1Cre_E12.5_4_25


title: Pdx1Cre_E12.5_4_17


title: Pdx1Cre_E12.5_4_21


title: Pdx1Cre_E12.5_4_20


title: Pdx1Cre_E12.5_4_19


title: Pdx1Cre_E12.5_4_23


title: Pdx1Cre_E12.5_4_18


title: Pdx1Cre_E12.5_4_15


title: Pdx1Cre_E12.5_4_14


title: Pdx1Cre_E12.5_4_16


title: Pdx1Cre_E12.5_4_13


title: Pdx1Cre_E12.5_4_31


title: Pdx1Cre_E12.5_4_09


title: Pdx1Cre_E12.5_4_08


title: Pdx1Cre_E12.5_4_10


title: Pdx1Cre_E12.5_4_06


title: Pdx1Cre_E12.5_4_05


title: Pdx1Cre_E12.5_4_03


title: Pdx1Cre_E12.5_4_04


title: Pdx1Cre_E12.5_4_02


title: S88

city: Bar Harbor

first: Catherine

middle: C

last: Kaczorowski

state: ME

address: 600 Main Street

institute: The Jackson Laboratory

data_processing:
Bowtie 1.1.2 alignment (bowtie -q -p 8 -a --best --strata --sam -v 3)
EMASE 0.10.16 for abundance measurements
TPM data was combined with GSE101144 and Ntg littermates from 3rd GEO accession (total n = 290) and batch corrected using ComBat. Samples S3 and S285 were excluded as outliers from final analysis (S3 prior to clusters, S285 post-clustering).
Genome_build: mm10
Supplementary_files_format_and_content:
genesTPM_Rd2_GEO_5XFAD.csv; direct TPM (normalized counts) output from EMASE quantification
genesERC_Rd2_GEO_5XFAD.csv; direct expected read count from EMASE quantification
genesTPM_allDataComBatCorrected_final.csv; ComBat corrected data for all combined data from GSE101144, this study, and 3rd Ntg-BXD GEO submission

growth_protocol: Animals were fear conditioning at either 6 or 14 months. Immediately following contextual fear memory testing, mice were euthanized by isoflurane, decaptitated, and brains were immediately harvested. Hippocampus was freshly dissected, snap frozen in liquid nitrogen, and stored at -80 until RNA extraction

extract_protocol:
RNEasy mini kit (QIAGEN) on QIACUBE (QIAGEN)
RNA was isolated on a Qiacube using the RNeasy mini kit (Qiagen) and treated with Dnase to remove contaminating DNA. RNA quality was confirmed using a BioAnalyzer (Agilent Technologies). All samples had RNA Integrity Numbers (RIN values) > 8.0. Sequencing libraries were prepared from 1 µg RNA with the Truseq Stranded mRNA Sample Preparation Kit (Illumina Inc) following the manufacturer’s protocol. Final PCR-enriched fragments were validated on a 2200 Tapestation Instrument using the D1000 ScreenTape (Agilent Technologies) and quantified by qPCR using a Universal Library Quantification Kit (Kapa Biosystems) on the QuantStudio 6 Flex (ThermoFisher Scientific). Final library pools were sequenced by 75bp paired-end sequencing on a HiSeq2500 (Illumina Inc).

treatment_protocol: Hippocampus was dissected from fresh tissue, snap frozen in liquid nitrogen, and stored at -80 until RNA extraction

tag: strain

tag: Sex

value: male

value: hippocampus


title: Pdx1Cre_E12.5_4_01

tag: tissue


title: S72


title: S6

value: female


title: S59


title: Pdx1Cre_E12.5_4_12


title: S48


title: S305


title: S303


title: S298


title: S293


title: S295


title: S289


title: S290


title: Pdx1Cre_E12.5_4_11


title: S292


title: Pdx1Cre_E12.5_4_07


title: S288


title: S284


title: S283


title: S286


title: S54


title: S277


title: S271


title: S306


title: S296


title: S269


title: S262


title: S275


title: S279


title: S260


title: S263


title: S259


title: S276


title: S257


title: S256


title: S252


title: S300


title: S253


title: S248


title: S250


title: S245


title: S282


title: S274


title: S280


title: S244


title: S240


title: S241


title: S235


title: S231


title: S267


title: S230


title: S224


title: S225


title: S227


title: S222


title: S218


title: S211


title: S217


title: S221


title: S209


title: S216


title: S214


title: S200


title: S204


title: S199


title: S238


title: S198


title: S192


title: S197


title: S196


title: S189


title: S190


title: S183


title: S180


title: S181


title: S177


title: S223


title: S176


title: S172


title: S171


title: S169


title: S168


title: S203


title: S143


title: S113


title: S146


title: S151


title: S125


title: LS-18-4_IN_MI-463

city: Vienna

first: Thomas

last: Eder

address: Veterinaerplatz 1

department: Department for Biological Sciences and Pathobiology

country: Austria

institute: University of Veterinary Medicine, Vienna

library_source: genomic

data_processing:
RNA-seq:
Quality was checked with FastQC
Quality-filtering and -trimming with PRINSEQ-lite
Mapping against the Mus musculus (GRCm38) reference genome with STAR
Mapped reads per gene were counted with featureCounts
Normalization and differential expression analysis was carried out with DESeq2
ChIP-seq:
Quality was checked with FastQC
Quality-filtering and -trimming with PRINSEQ-lite
Mapping against the Mus musculus (GRCm38) reference genome via BWA
Post-processing with SAMtools
RPKM normalization and conversion to BigWig files was carried out with Deeptools
Peak calling for the C/EBPa p30 ChIP-seq dataset was done with MACS2
Peak calling for the MLL ChIP-seq data was done with Sicer
Called peaks were annotated with Homer
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include normalized expression values for each sample

molecule: genomic DNA

source_name: myeloid-progenitor-derived cell line

growth_protocol: p30/p30 cells were cultivated in RPMI 640 supplemented with 10% FCS and 2 mM glutamine, 100 U/ml penicillin and 100 µg/ml streptomycin. 5 ng/ml mIL-3 was added every 2-3 days.

extract_protocol:
For RNA-seq QIAGEN RNeasy Plus Kit was used, and for ChIP-seq, lysates were sonicated and histone-DNA complexes were isolated with antibody.
Libraries were prepared for sequencing using standard Illumina protocols by sequencing facilities.

treatment_protocol: Samples DMSO, MI-463 and MI-503 were treated at 0h, 48h and taken at 72h.

tag: genotype

tag: chip antibody


title: LS-18-5_IN_DMSO


title: LS-18-5_C-18_1_DMSO

value: C/EBPα C-18 (Santa Cruz, sc-9314)


title: LS-18-4_IN_DMSO


title: LS-18-5_C-18_2_DMSO


title: LS-18-4_MLL_DMSO

value: anti-MLL1 (Bethyl Laboratories, A300-086A)


title: LS-18-4_MLL_MI-463


title: MI-503 rep1


title: MI-503 rep3


title: MI-463 rep3


title: MI-463 rep2


title: MI-463 rep1


title: DMSO rep3


title: DMSO rep1


title: U0126_Ins1Pos_E13.0_CultureFor2Days_3_19

source_name: Pancreas explant


title: U0126_Ins1Pos_E13.0_CultureFor2Days_3_18


title: U0126_Ins1Pos_E13.0_CultureFor2Days_3_16


title: U0126_Ins1Pos_E13.0_CultureFor2Days_3_20


title: U0126_Ins1Pos_E13.0_CultureFor2Days_3_17


title: S137

tag: age


title: S205


title: U0126_Ins1Pos_E13.0_CultureFor2Days_3_14


title: U0126_Ins1Pos_E13.0_CultureFor2Days_3_11


title: U0126_Ins1Pos_E13.0_CultureFor2Days_3_12


title: U0126_Ins1Pos_E13.0_CultureFor2Days_3_10


title: U0126_Ins1Pos_E13.0_CultureFor2Days_3_13


title: DMSO rep2


title: MI-503 rep2


title: U0126_Ins1Pos_E13.0_CultureFor2Days_3_09


title: U0126_Ins1Pos_E13.0_CultureFor2Days_3_08


title: U0126_Ins1Pos_E13.0_CultureFor2Days_3_07


title: U0126_Ins1Pos_E13.0_CultureFor2Days_3_05


title: U0126_Ins1Pos_E13.0_CultureFor2Days_3_06


title: U0126_Ins1Pos_E13.0_CultureFor2Days_3_01


title: U0126_Ins1Pos_E13.0_CultureFor2Days_3_04


title: U0126_Ins1Pos_E13.0_CultureFor2Days_3_03


title: U0126_Ins1Pos_E13.0_CultureFor2Days_3_02


title: U0126_Ins1Pos_E13.0_CultureFor2Days_2_10


title: U0126_Ins1Pos_E13.0_CultureFor2Days_2_13


title: U0126_Ins1Pos_E13.0_CultureFor2Days_2_11


title: U0126_Ins1Pos_E13.0_CultureFor2Days_2_12


title: U0126_Ins1Pos_E13.0_CultureFor2Days_2_08


title: U0126_Ins1Pos_E13.0_CultureFor2Days_2_07


title: U0126_Ins1Pos_E13.0_CultureFor2Days_2_09


title: U0126_Ins1Pos_E13.0_CultureFor2Days_2_04


title: U0126_Ins1Pos_E13.0_CultureFor2Days_2_06


title: U0126_Ins1Pos_E13.0_CultureFor2Days_2_02


title: U0126_Ins1Pos_E13.0_CultureFor2Days_2_03


title: U0126_Ins1Pos_E13.0_CultureFor2Days_1_07


title: U0126_Ins1Pos_E13.0_CultureFor2Days_1_03


title: U0126_Ins1Pos_E13.0_CultureFor2Days_1_02


title: U0126_Ins1Pos_E13.0_CultureFor2Days_2_01


title: U0126_Ins1Pos_E13.0_CultureFor2Days_1_04


title: U0126_Ins1Pos_E13.0_CultureFor2Days_1_01


title: U0126_Ins1Pos_E13.0_CultureFor2Days_3_15


title: U0126_Ins1Pos_E13.0_CultureFor2Days_1_06


title: Control_Ins1Pos_E13.0_CultureFor2Days_3_17


title: Control_Ins1Pos_E13.0_CultureFor2Days_3_13


title: Control_Ins1Pos_E13.0_CultureFor2Days_3_14


title: Control_Ins1Pos_E13.0_CultureFor2Days_3_18


title: Control_Ins1Pos_E13.0_CultureFor2Days_3_12


title: U0126_Ins1Pos_E13.0_CultureFor2Days_1_05


title: S255


title: Control_Ins1Pos_E13.0_CultureFor2Days_3_15


title: Control_Ins1Pos_E13.0_CultureFor2Days_3_11


title: Control_Ins1Pos_E13.0_CultureFor2Days_3_06


title: Control_Ins1Pos_E13.0_CultureFor2Days_3_04


title: Control_Ins1Pos_E13.0_CultureFor2Days_3_09


title: Control_Ins1Pos_E13.0_CultureFor2Days_3_07


title: Control_Ins1Pos_E13.0_CultureFor2Days_3_08


title: Control_Ins1Pos_E13.0_CultureFor2Days_2_12


title: Control_Ins1Pos_E13.0_CultureFor2Days_3_03


title: Control_Ins1Pos_E13.0_CultureFor2Days_3_02


title: Control_Ins1Pos_E13.0_CultureFor2Days_2_11


title: U0126_Ins1Pos_E13.0_CultureFor2Days_2_05


title: Control_Ins1Pos_E13.0_CultureFor2Days_3_01


title: Control_Ins1Pos_E13.0_CultureFor2Days_2_10


title: Control_Ins1Pos_E13.0_CultureFor2Days_3_05


title: Control_Ins1Pos_E13.0_CultureFor2Days_3_16


title: Control_Ins1Pos_E13.0_CultureFor2Days_3_10


title: Control_Ins1Pos_E13.0_CultureFor2Days_2_09


title: Control_Ins1Pos_E13.0_CultureFor2Days_2_02


title: Control_Ins1Pos_E13.0_CultureFor2Days_2_06


title: Control_Ins1Pos_E13.0_CultureFor2Days_2_07


title: Control_Ins1Pos_E13.0_CultureFor2Days_2_08


title: Control_Ins1Pos_E13.0_CultureFor2Days_2_03


title: Control_Ins1Pos_E13.0_CultureFor2Days_2_05


title: Control_Ins1Pos_E13.0_CultureFor2Days_1_04


title: Control_Ins1Pos_E13.0_CultureFor2Days_1_03


title: Control_Ins1Pos_E13.0_CultureFor2Days_1_08


title: GcgPos_E17.5_10

value: Gcg-GFP+


title: Control_Ins1Pos_E13.0_CultureFor2Days_2_01


title: Control_Ins1Pos_E13.0_CultureFor2Days_1_07


title: Control_Ins1Pos_E13.0_CultureFor2Days_1_01


title: GcgPos_E17.5_11


title: GcgPos_E17.5_08


title: GcgPos_E17.5_06


title: GcgPos_E17.5_04


title: GcgPos_E17.5_07


title: GcgPos_E17.5_05


title: GcgPos_E17.5_03


title: GcgPos_E17.5_02


title: GcgPos_E17.5_01


title: Control_Ins1Pos_E13.0_CultureFor2Days_2_04


title: Control_Ins1Pos_E13.0_CultureFor2Days_1_05


title: Control_Ins1Pos_E13.0_CultureFor2Days_1_02


title: Sox9CreER_E17.5_2_23


title: Sox9CreER_E17.5_2_21


title: Sox9CreER_E17.5_2_17


title: Sox9CreER_E17.5_2_18


title: Sox9CreER_E17.5_2_22


title: Sox9CreER_E17.5_2_16


title: Sox9CreER_E17.5_2_13


title: GcgPos_E17.5_09


title: Sox9CreER_E17.5_2_15


title: Sox9CreER_E17.5_2_12


title: Sox9CreER_E17.5_2_14


title: Sox9CreER_E17.5_2_10


title: Sox9CreER_E17.5_2_20


title: Sox9CreER_E17.5_2_09


title: Sox9CreER_E17.5_2_08


title: Sox9CreER_E17.5_2_05


title: Sox9CreER_E17.5_2_11


title: Sox9CreER_E17.5_2_04


title: Control_Ins1Pos_E13.0_CultureFor2Days_1_06


title: Sox9CreER_E17.5_2_07


title: Sox9CreER_E17.5_2_03


title: Sox9CreER_E17.5_2_02


title: Sox9CreER_E17.5_1_10


title: Sox9CreER_E17.5_2_24


title: Sox9CreER_E17.5_1_08


title: Sox9CreER_E17.5_2_01


title: Sox9CreER_E17.5_1_09


title: Sox9CreER_E17.5_2_19


title: Sox9CreER_E17.5_1_07


title: Sox9CreER_E17.5_1_04


title: Sox9CreER_E17.5_1_02


title: Sox9CreER_E17.5_1_05


title: Sox9CreER_E17.5_1_01


title: Sox9CreER_E16.5_2_30


title: Sox9CreER_E16.5_2_29


title: Sox9CreER_E17.5_1_06


title: Sox9CreER_E16.5_2_23


title: Sox9CreER_E16.5_2_25


title: Sox9CreER_E17.5_1_03


title: Sox9CreER_E16.5_2_22


title: Sox9CreER_E16.5_2_24


title: Sox9CreER_E16.5_2_28


title: Sox9CreER_E16.5_2_26


title: Sox9CreER_E16.5_2_20


title: Sox9CreER_E16.5_2_17


title: Sox9CreER_E17.5_2_06


title: Sox9CreER_E16.5_2_14


title: Sox9CreER_E16.5_2_21


title: Sox9CreER_E16.5_2_19


title: Sox9CreER_E16.5_2_13


title: Sox9CreER_E16.5_2_18


title: Sox9CreER_E16.5_2_12


title: Sox9CreER_E16.5_2_10


title: Sox9CreER_E16.5_2_09


title: Sox9CreER_E16.5_2_08


title: Sox9CreER_E16.5_2_07


title: Sox9CreER_E16.5_2_02


title: Sox9CreER_E16.5_2_05


title: Sox9CreER_E16.5_2_03


title: Sox9CreER_E16.5_2_04


title: Sox9CreER_E16.5_2_27


title: Sox9CreER_E16.5_2_06


title: Sox9CreER_E16.5_2_01


title: Sox9CreER_E16.5_1_25


title: Sox9CreER_E16.5_1_22


title: Sox9CreER_E16.5_1_21


title: Sox9CreER_E16.5_1_24


title: Sox9CreER_E16.5_2_16


title: Sox9CreER_E16.5_1_20


title: Sox9CreER_E16.5_1_23


title: Sox9CreER_E16.5_2_15


title: Sox9CreER_E16.5_1_16


title: Sox9CreER_E16.5_1_17


title: Sox9CreER_E16.5_2_11


title: Sox9CreER_E16.5_1_14


title: Sox9CreER_E16.5_1_18


title: Sox9CreER_E16.5_1_15


title: Sox9CreER_E16.5_1_13


title: Sox9CreER_E16.5_1_09


title: Sox9CreER_E16.5_1_10


title: Sox9CreER_E16.5_1_08


title: Sox9CreER_E16.5_1_12


title: Sox9CreER_E16.5_1_07


title: Sox9CreER_E16.5_1_06


title: Sox9CreER_E16.5_1_05


title: Sox9CreER_E16.5_1_04


title: Pdx1Pos_E9.5_3_31


title: Sox9CreER_E16.5_1_02


title: Sox9CreER_E16.5_1_01


title: Sox9CreER_E16.5_1_19


title: Pdx1Pos_E9.5_3_29


title: Pdx1Pos_E9.5_3_30


title: Pdx1Pos_E9.5_3_25


title: Pdx1Pos_E9.5_3_26


title: Pdx1Pos_E9.5_3_22


title: Pdx1Pos_E9.5_3_27


title: Pdx1Pos_E9.5_3_21


title: Pdx1Pos_E9.5_3_23


title: Pdx1Pos_E9.5_3_24


title: Pdx1Pos_E9.5_3_18


title: Sox9CreER_E16.5_1_11


title: Pdx1Pos_E9.5_3_19


title: Pdx1Pos_E9.5_3_16


title: Pdx1Pos_E9.5_3_20


title: Pdx1Pos_E9.5_3_17


title: Pdx1Pos_E9.5_3_15


title: Pdx1Pos_E9.5_3_11


title: Sox9CreER_E16.5_1_03


title: Pdx1Pos_E9.5_3_12


title: Pdx1Pos_E9.5_3_13


title: Pdx1Pos_E9.5_3_09


title: Pdx1Pos_E9.5_3_08


title: Pdx1Pos_E9.5_3_10


title: Pdx1Pos_E9.5_3_07


title: Pdx1Pos_E9.5_3_06


title: Pdx1Pos_E9.5_3_05


title: Pdx1Pos_E9.5_3_14


title: Pdx1Pos_E9.5_3_04


title: Pdx1Pos_E9.5_3_28


title: Pdx1Pos_E9.5_3_03


title: Pdx1Pos_E9.5_3_02


title: Pdx1Pos_E9.5_2_25


title: Pdx1Pos_E9.5_2_23


title: Pdx1Pos_E9.5_2_24


title: Pdx1Pos_E9.5_2_21


title: Pdx1Pos_E9.5_3_01


title: Pdx1Pos_E9.5_2_18


title: Pdx1Pos_E9.5_2_17


title: Pdx1Pos_E9.5_2_14


title: Pdx1Pos_E9.5_2_10


title: Pdx1Pos_E9.5_2_09


title: Pdx1Pos_E9.5_2_08


title: Pdx1Pos_E9.5_2_16


title: Pdx1Pos_E9.5_2_03


title: Pdx1Pos_E9.5_2_13


title: Pdx1Pos_E9.5_2_01


title: Pdx1Pos_E9.5_2_02


title: Pdx1Pos_E9.5_1_57


title: Pdx1Pos_E9.5_2_11


title: Pdx1Pos_E9.5_1_55


title: Pdx1Pos_E9.5_2_05


title: Pdx1Pos_E9.5_2_22


title: Pdx1Pos_E9.5_2_06


title: Pdx1Pos_E9.5_2_20


title: Pdx1Pos_E9.5_2_19


title: Pdx1Pos_E9.5_1_53


title: Pdx1Pos_E9.5_1_54


title: Pdx1Pos_E9.5_2_15


title: Pdx1Pos_E9.5_1_52


title: Pdx1Pos_E9.5_1_47


title: Pdx1Pos_E9.5_2_12


title: Pdx1Pos_E9.5_1_50


title: Pdx1Pos_E9.5_1_43


title: Pdx1Pos_E9.5_1_48


title: Pdx1Pos_E9.5_2_07


title: Pdx1Pos_E9.5_1_46


title: Pdx1Pos_E9.5_1_51


title: Pdx1Pos_E9.5_1_45


title: Pdx1Pos_E9.5_2_04


title: Pdx1Pos_E9.5_1_56


title: Pdx1Pos_E9.5_1_44


title: Pdx1Pos_E9.5_1_39


title: Pdx1Pos_E9.5_1_37


title: Pdx1Pos_E9.5_1_33


title: Pdx1Pos_E9.5_1_31


title: Pdx1Pos_E9.5_1_30


title: Pdx1Pos_E9.5_1_36


title: Pdx1Pos_E9.5_1_42


title: Pdx1Pos_E9.5_1_41


title: Pdx1Pos_E9.5_1_38


title: Pdx1Pos_E9.5_1_32


title: Pdx1Pos_E9.5_1_26


title: Pdx1Pos_E9.5_1_49


title: Pdx1Pos_E9.5_1_34


title: Pdx1Pos_E9.5_1_35


title: Pdx1Pos_E9.5_1_24


title: Pdx1Pos_E9.5_1_22


title: Pdx1Pos_E9.5_1_19


title: Pdx1Pos_E9.5_1_40


title: Pdx1Pos_E9.5_1_15


title: Pdx1Pos_E9.5_1_14


title: Pdx1Pos_E9.5_1_27


title: Pdx1Pos_E9.5_1_20


title: Pdx1Pos_E9.5_1_23


title: Pdx1Pos_E9.5_1_12


title: Pdx1Pos_E9.5_1_17


title: Pdx1Pos_E9.5_1_16


title: Pdx1Pos_E9.5_1_25


title: Pdx1Pos_E9.5_1_11


title: Pdx1Pos_E9.5_1_28


title: Pdx1Pos_E9.5_1_29


title: Pdx1Pos_E9.5_1_10


title: Pdx1Pos_E9.5_1_13


title: Pdx1Pos_E9.5_1_09


title: Pdx1Pos_E9.5_1_05


title: Pdx1Pos_E9.5_1_02


title: Pdx1Pos_E9.5_1_21


title: Pdx1Pos_E9.5_1_08


title: Pdx1Pos_E9.5_1_03


title: Pdx1Pos_E11.5_2_29


title: Pdx1Pos_E11.5_2_28


title: Pdx1Pos_E9.5_1_04


title: Pdx1Pos_E9.5_1_06


title: Pdx1Pos_E9.5_1_01


title: Pdx1Pos_E9.5_1_07


title: Pdx1Pos_E9.5_1_18


title: Pdx1Pos_E11.5_2_24


title: Pdx1Pos_E11.5_2_25


title: Pdx1Pos_E11.5_2_27


title: Pdx1Pos_E11.5_2_23


title: Pdx1Pos_E11.5_2_21


title: Pdx1Pos_E11.5_2_20


title: Pdx1Pos_E11.5_2_22


title: Pdx1Pos_E11.5_2_17


title: Pdx1Pos_E11.5_2_19


title: Pdx1Pos_E11.5_2_18


title: Pdx1Pos_E11.5_2_16


title: Pdx1Pos_E11.5_2_15


title: Pdx1Pos_E11.5_2_14


title: Pdx1Pos_E11.5_2_12


title: Pdx1Pos_E11.5_2_13


title: Pdx1Pos_E11.5_2_08


title: Pdx1Pos_E11.5_2_11


title: Pdx1Pos_E11.5_2_10


title: Pdx1Pos_E11.5_2_26


title: Pdx1Pos_E11.5_2_02


title: Pdx1Pos_E11.5_2_03


title: Pdx1Pos_E11.5_1_31


title: Pdx1Pos_E11.5_1_30


title: Pdx1Pos_E11.5_2_06


title: Pdx1Pos_E11.5_2_05


title: Pdx1Pos_E11.5_2_07


title: Pdx1Pos_E11.5_2_04


title: Pdx1Pos_E11.5_1_29


title: Pdx1Pos_E11.5_1_27


title: Pdx1Pos_E11.5_1_26


title: Pdx1Pos_E11.5_1_28


title: Pdx1Pos_E11.5_1_25


title: Pdx1Pos_E11.5_1_23


title: Pdx1Pos_E11.5_1_24


title: Pdx1Pos_E11.5_1_19


title: Pdx1Pos_E11.5_1_21


title: Pdx1Pos_E11.5_1_18


title: Pdx1Pos_E11.5_1_22


title: Pdx1Pos_E11.5_1_20


title: Pdx1Pos_E11.5_2_09


title: Pdx1Pos_E11.5_1_17


title: Pdx1Pos_E11.5_1_16


title: Pdx1Pos_E11.5_1_11


title: Pdx1Pos_E11.5_1_13


title: Pdx1Pos_E11.5_1_15


title: Pdx1Pos_E11.5_1_14


title: Pdx1Pos_E11.5_2_01


title: Pdx1Pos_E11.5_1_12


title: Pdx1Pos_E11.5_1_08


title: Pdx1Pos_E11.5_1_10


title: Pdx1Pos_E11.5_1_05


title: Pdx1Pos_E11.5_1_06


title: Pdx1Pos_E11.5_1_03


title: Pdx1Pos_E11.5_1_02


title: Pdx1Pos_E11.5_1_07


title: Pdx1Pos_E11.5_1_04


title: Pdx1Pos_E11.5_1_09


title: Pdx1Pos_E10.5_4_17


title: Pdx1Pos_E11.5_1_01


title: Pdx1Pos_E10.5_4_15


title: Pdx1Pos_E10.5_4_16


title: Pdx1Pos_E10.5_4_12


title: Pdx1Pos_E10.5_4_13


title: Pdx1Pos_E10.5_4_14


title: Pdx1Pos_E10.5_4_08


title: Pdx1Pos_E10.5_4_07


title: Pdx1Pos_E10.5_4_10


title: Pdx1Pos_E10.5_4_09


title: Pdx1Pos_E10.5_4_11


title: Pdx1Pos_E10.5_4_06


title: Pdx1Pos_E10.5_4_02


title: Pdx1Pos_E10.5_4_03


title: Pdx1Pos_E10.5_4_01


title: Pdx1Pos_E10.5_4_05


title: Pdx1Pos_E10.5_4_04


title: Pdx1Pos_E10.5_3_22


title: Pdx1Pos_E10.5_3_25


title: Pdx1Pos_E10.5_3_21


title: Pdx1Pos_E10.5_3_23


title: Pdx1Pos_E10.5_3_19


title: Pdx1Pos_E10.5_3_20


title: Pdx1Pos_E10.5_3_24


title: Pdx1Pos_E10.5_3_16


title: Pdx1Pos_E10.5_3_14


title: Pdx1Pos_E10.5_3_13


title: Pdx1Pos_E10.5_3_15


title: Pdx1Pos_E10.5_3_17


title: Pdx1Pos_E10.5_3_12


title: Pdx1Pos_E10.5_3_11


title: Pdx1Pos_E10.5_3_09


title: Pdx1Pos_E10.5_3_07


title: Pdx1Pos_E10.5_3_08


title: Pdx1Pos_E10.5_3_06


title: Pdx1Pos_E10.5_3_04


title: Pdx1Pos_E10.5_3_03


title: Pdx1Pos_E10.5_3_05


title: Pdx1Pos_E10.5_3_01


title: Pdx1High_E10.5_2_12


title: Pdx1High_E10.5_2_13


title: Pdx1High_E10.5_2_11


title: Pdx1High_E10.5_2_15


title: Pdx1Pos_E10.5_3_18


title: Pdx1High_E10.5_2_16


title: Pdx1Pos_E10.5_3_02


title: Pdx1High_E10.5_2_14


title: Pdx1High_E10.5_2_05


title: Pdx1High_E10.5_2_07


title: Pdx1Pos_E10.5_3_10


title: Pdx1High_E10.5_2_06


title: Pdx1High_E10.5_2_10


title: Pdx1High_E10.5_2_09


title: Pdx1High_E10.5_2_02


title: Pdx1High_E10.5_2_01


title: Pdx1High_E10.5_1_11


title: Pdx1High_E10.5_1_10


title: Pdx1High_E10.5_1_09


title: Pdx1High_E10.5_1_08


title: Pdx1High_E10.5_1_06


title: Pdx1High_E10.5_1_04


title: Pdx1High_E10.5_1_05


title: Pdx1High_E10.5_1_03


title: Pdx1High_E10.5_1_02


title: Pdx1High_E10.5_2_08


title: Pdx1High_E10.5_1_01


title: Pdx1Cre_E15.5_3_45


title: Pdx1Cre_E15.5_3_47


title: Pdx1Cre_E15.5_3_42


title: Pdx1Cre_E15.5_3_46


title: Pdx1Cre_E15.5_3_44


title: Pdx1High_E10.5_2_04


title: Pdx1Cre_E15.5_3_37


title: Pdx1High_E10.5_2_03


title: Pdx1Cre_E15.5_3_43


title: Pdx1Cre_E15.5_3_38


title: Pdx1Cre_E15.5_3_33


title: Pdx1Cre_E15.5_3_34


title: Pdx1Cre_E15.5_3_35


title: Pdx1Cre_E15.5_3_41


title: Pdx1Cre_E15.5_3_39


title: Pdx1Cre_E15.5_3_40


title: Pdx1High_E10.5_1_07


title: Pdx1Cre_E15.5_3_32


title: Pdx1Cre_E15.5_3_29


title: Pdx1Cre_E15.5_3_30


title: Pdx1Cre_E15.5_3_31


title: Pdx1Cre_E15.5_3_28


title: Pdx1Cre_E15.5_3_26


title: Pdx1Cre_E15.5_3_24


title: Pdx1Cre_E15.5_3_25


title: Pdx1Cre_E15.5_3_21


title: Pdx1Cre_E15.5_3_22


title: Pdx1Cre_E15.5_3_20


title: Pdx1Cre_E15.5_3_36


title: Pdx1Cre_E15.5_3_19


title: Pdx1Cre_E15.5_3_15


title: Pdx1Cre_E15.5_3_17


title: Pdx1Cre_E15.5_3_16


title: Pdx1Cre_E15.5_3_18


title: Pdx1Cre_E15.5_3_11


title: Pdx1Cre_E15.5_3_09


title: Pdx1Cre_E15.5_3_10


title: Pdx1Cre_E15.5_3_14


title: Pdx1Cre_E15.5_3_05


title: Pdx1Cre_E15.5_3_06


title: Pdx1Cre_E15.5_3_12


title: Pdx1Cre_E15.5_3_07


title: Pdx1Cre_E15.5_3_27


title: Pdx1Cre_E15.5_3_13


title: Pdx1Cre_E15.5_3_04


title: Pdx1Cre_E15.5_3_08


title: Pdx1Cre_E15.5_3_02


title: Pdx1Cre_E15.5_2_08


title: Pdx1Cre_E15.5_2_07


title: Pdx1Cre_E15.5_3_01


title: Pdx1Cre_E15.5_2_06


title: Pdx1Cre_E15.5_2_05


title: Pdx1Cre_E15.5_2_04


title: Pdx1Cre_E15.5_3_23


title: Pdx1Cre_E15.5_2_03


title: Pdx1Cre_E15.5_2_02


title: Pdx1Cre_E15.5_2_01


title: Pdx1Cre_E15.5_1_24


title: Pdx1Cre_E15.5_1_23


title: Pdx1Cre_E15.5_1_20


title: Pdx1Cre_E15.5_1_22


title: Pdx1Cre_E15.5_1_21


title: Pdx1Cre_E15.5_1_19


title: Pdx1Cre_E15.5_3_03


title: Pdx1Cre_E15.5_1_15


title: Pdx1Cre_E15.5_1_14


title: Pdx1Cre_E15.5_1_12


title: Pdx1Cre_E15.5_1_13


title: Pdx1Cre_E15.5_1_17


title: Pdx1Cre_E15.5_1_11


title: Pdx1Cre_E15.5_1_16


title: Pdx1Cre_E15.5_1_06


title: Pdx1Cre_E15.5_1_10


title: Pdx1Cre_E15.5_1_09


title: Pdx1Cre_E15.5_1_08


title: Pdx1Cre_E15.5_1_04


title: Pdx1Cre_E15.5_1_02


title: Pdx1Cre_E15.5_1_03


title: Pdx1Cre_E14.5_3_61


title: Pdx1Cre_E14.5_3_60


title: Pdx1Cre_E14.5_3_59


title: Pdx1Cre_E14.5_3_56


title: Pdx1Cre_E14.5_3_57


title: Pdx1Cre_E14.5_3_55


title: Pdx1Cre_E14.5_3_54


title: Pdx1Cre_E14.5_3_58


title: Pdx1Cre_E15.5_1_18


title: Pdx1Cre_E14.5_3_52


title: Pdx1Cre_E14.5_3_49


title: Pdx1Cre_E15.5_1_05


title: Pdx1Cre_E14.5_3_47


title: Pdx1Cre_E14.5_3_45


title: Pdx1Cre_E14.5_3_53


title: Pdx1Cre_E14.5_3_48


title: Pdx1Cre_E14.5_3_50


title: Pdx1Cre_E15.5_1_07


title: Pdx1Cre_E15.5_1_01


title: Pdx1Cre_E14.5_3_41


title: Pdx1Cre_E14.5_3_51


title: Pdx1Cre_E14.5_3_40


title: Pdx1Cre_E14.5_3_36


title: Pdx1Cre_E14.5_3_35


title: Pdx1Cre_E14.5_3_46


title: Pdx1Cre_E14.5_3_37


title: Pdx1Cre_E14.5_3_34


title: Pdx1Cre_E14.5_3_38


title: Pdx1Cre_E14.5_3_39


title: Pdx1Cre_E14.5_3_43


title: Pdx1Cre_E14.5_3_33


title: Pdx1Cre_E14.5_3_32


title: Pdx1Cre_E14.5_3_27


title: Pdx1Cre_E14.5_3_30


title: Pdx1Cre_E14.5_3_28


title: Pdx1Cre_E14.5_3_44


title: Pdx1Cre_E14.5_3_42


title: Pdx1Cre_E14.5_3_23


title: Pdx1Cre_E14.5_3_21


title: Pdx1Cre_E14.5_3_22


title: Pdx1Cre_E14.5_3_25


title: Pdx1Cre_E14.5_3_24


title: Pdx1Cre_E14.5_3_16


title: Pdx1Cre_E14.5_3_17


title: Pdx1Cre_E14.5_3_19


title: Pdx1Cre_E14.5_3_18


title: Pdx1Cre_E14.5_3_15


title: Pdx1Cre_E14.5_3_13


title: Pdx1Cre_E14.5_3_14


title: Pdx1Cre_E14.5_3_29


title: Pdx1Cre_E14.5_3_11


title: Pdx1Cre_E14.5_3_09


title: Pdx1Cre_E14.5_3_31


title: Pdx1Cre_E14.5_3_04


title: Pdx1Cre_E14.5_3_07


title: Pdx1Cre_E14.5_3_06


title: Pdx1Cre_E14.5_3_05


title: Pdx1Cre_E14.5_3_26


title: Pdx1Cre_E14.5_3_03


title: Pdx1Cre_E14.5_2_15


title: Pdx1Cre_E14.5_3_02


title: Pdx1Cre_E14.5_3_01


title: Pdx1Cre_E14.5_3_20


title: Pdx1Cre_E14.5_2_14


title: Pdx1Cre_E14.5_2_10


title: Pdx1Cre_E14.5_2_11


title: Pdx1Cre_E14.5_2_13


title: Pdx1Cre_E14.5_2_12


title: Pdx1Cre_E14.5_2_08


title: Pdx1Cre_E14.5_3_10


title: Pdx1Cre_E14.5_2_06


title: Pdx1Cre_E14.5_2_09


title: Pdx1Cre_E14.5_2_07


title: Pdx1Cre_E14.5_3_12


title: Pdx1Cre_E14.5_3_08


title: Pdx1Cre_E14.5_2_03


title: Pdx1Cre_E14.5_2_02


title: Pdx1Cre_E14.5_1_05


title: Pdx1Cre_E14.5_2_05


title: Pdx1Cre_E14.5_1_04


title: Pdx1Cre_E14.5_1_06


title: Pdx1Cre_E14.5_2_01


title: Pdx1Cre_E14.5_1_03


title: Pdx1Cre_E14.5_1_02


title: Pdx1Cre_E13.5_4_24


title: Pdx1Cre_E13.5_4_21


title: Pdx1Cre_E13.5_4_23


title: Pdx1Cre_E13.5_4_19


title: Pdx1Cre_E13.5_4_16


title: Pdx1Cre_E13.5_4_22


title: Pdx1Cre_E13.5_4_14


title: Pdx1Cre_E13.5_4_18


title: Pdx1Cre_E13.5_4_17


title: Pdx1Cre_E13.5_4_13


title: Pdx1Cre_E14.5_2_04


title: Pdx1Cre_E13.5_4_15


title: Pdx1Cre_E13.5_4_11


title: Pdx1Cre_E13.5_4_07


title: Pdx1Cre_E13.5_4_06


title: Pdx1Cre_E13.5_4_08


title: Pdx1Cre_E13.5_4_10


title: Pdx1Cre_E13.5_4_12


title: Pdx1Cre_E13.5_4_09


title: Pdx1Cre_E13.5_4_04


title: Pdx1Cre_E13.5_4_01


title: Pdx1Cre_E13.5_4_03


title: Pdx1Cre_E13.5_3_18


title: Pdx1Cre_E13.5_4_02


title: Pdx1Cre_E13.5_3_19


title: Pdx1Cre_E13.5_3_20


title: Pdx1Cre_E14.5_1_01


title: Pdx1Cre_E13.5_4_20


title: Pdx1Cre_E13.5_3_16


title: Pdx1Cre_E13.5_3_17


title: Pdx1Cre_E13.5_3_14


title: Pdx1Cre_E13.5_3_15


title: Pdx1Cre_E13.5_3_13


title: Pdx1Cre_E13.5_3_06


title: Pdx1Cre_E13.5_3_09


title: Pdx1Cre_E13.5_3_07


title: Pdx1Cre_E13.5_3_08


title: Pdx1Cre_E13.5_3_12


title: Pdx1Cre_E13.5_3_10


title: Pdx1Cre_E13.5_3_11


title: Pdx1Cre_E13.5_4_05


title: Pdx1Cre_E13.5_3_01


title: Pdx1Cre_E13.5_3_03


title: Pdx1Cre_E13.5_3_04


title: Pdx1Cre_E13.5_2_32


title: Pdx1Cre_E13.5_2_30


title: Pdx1Cre_E13.5_3_02


title: Pdx1Cre_E13.5_2_26


title: Pdx1Cre_E13.5_2_25


title: Pdx1Cre_E13.5_2_31


title: Pdx1Cre_E13.5_2_29


title: Pdx1Cre_E13.5_2_28


title: Pdx1Cre_E13.5_2_23


title: Pdx1Cre_E13.5_2_24


title: Pdx1Cre_E13.5_2_22


title: Pdx1Cre_E13.5_2_27


title: Pdx1Cre_E13.5_2_19


title: Pdx1Cre_E13.5_2_18


title: Pdx1Cre_E13.5_2_21


title: Pdx1Cre_E13.5_2_16


title: Pdx1Cre_E13.5_2_17


title: Pdx1Cre_E13.5_3_05


title: Pdx1Cre_E13.5_2_13


title: Pdx1Cre_E13.5_2_11


title: Pdx1Cre_E13.5_2_15


title: Pdx1Cre_E13.5_2_10


title: Pdx1Cre_E13.5_2_14


title: Pdx1Cre_E13.5_2_09


title: Pdx1Cre_E13.5_2_12


title: Pdx1Cre_E13.5_2_08


title: Pdx1Cre_E13.5_2_06


title: Pdx1Cre_E13.5_2_05


title: Pdx1Cre_E13.5_2_03


title: Pdx1Cre_E13.5_2_07


title: Pdx1Cre_E13.5_2_04


title: Pdx1Cre_E13.5_1_32


title: Pdx1Cre_E13.5_2_02


title: Pdx1Cre_E13.5_1_31


title: Pdx1Cre_E13.5_1_29


title: Pdx1Cre_E13.5_1_30


title: Pdx1Cre_E13.5_1_25


title: Pdx1Cre_E13.5_2_20


title: Pdx1Cre_E13.5_1_26


title: Pdx1Cre_E13.5_1_20


title: Ngn3Pos_E10.5_2_03


title: Pdx1Cre_E13.5_1_27


title: Pdx1Cre_E13.5_2_01


title: Pdx1Cre_E13.5_1_19


title: Pdx1Cre_E13.5_1_21


title: Pdx1Cre_E13.5_1_24


title: Pdx1Cre_E13.5_1_23


title: Pdx1Cre_E13.5_1_22


title: Ngn3Pos_E10.5_2_01


title: Ngn3Pos_E10.5_2_05


title: Ngn3Pos_E10.5_1_21


title: Ngn3Pos_E10.5_1_20


title: Pdx1Cre_E13.5_1_18


title: Ngn3Pos_E10.5_1_19


title: Ngn3Pos_E10.5_2_04


title: Ngn3Pos_E10.5_2_06


title: Ngn3Pos_E10.5_2_02


title: Ngn3Pos_E10.5_1_18


title: Ngn3Pos_E10.5_1_17