title: ALY KD PolyA-

city: Shanghai

first: Hong

last: Cheng

address: 320 YueYang Road

department: Shanghai Institute of Biochemistry and Cell Biology

institute: Shanghai Institutes for Biological Sciences

data_processing:
Basecalls performed using TopHat2.0,fastx_toolkit-0.0.13.2 (Q33)
RNA-seq reads were cleaned and mapped the human genome (hg19 version) TopHat2.0. Reads mapping to multiple independent genomic locations were assigned to the most abundant biotype of the locations. Genome annotations from RefSeq and ENSEMBL were downloaded from the UCSC genome browser website, and supplemented with published datasets.
To compare the expression of individual RNAs, we calculated the corrected reads per million (RPM) of each transcript. Fold changes were computed from the average normalized read coverage per million bases for ensemble gene.
Genome_build: hg19
Supplementary_files_format_and_content: tab-delimited excel files include RPM values for each Sample

source_name: uterine cervix

growth_protocol: HeLa cells were cultured in DMEM supplemented with 10% FBS (Biochrom).

extract_protocol:
The total RNA was extracted using the TRIzol,then the polyA+ RNA was isolated using the oligo(dT)-conjugated magnetic beads from the total RNA. The flowthrough  devoided of the rRNA was the polyA- RNA.
stranded cDNA libraries were generated with TruSeq Stranded mRNA Sample Prep Kit (Illumina) according to the manufacturer’s instructions.

treatment_protocol: For siRNA transfection, siRNA were transfected with lipofectamine RNAiMax (Invitrogen) for 72 hr following the manufacture's instructionfor.

value: HeLa cell

tag: tissue

value: uterine cervix

tag: genotype/variation

value: ALYREF KD

tag: isolation

value: polyA- RNA


title: 27_Cont-H63-Seedling-6dat-3

status: Public on Feb 27 2019

type: SRA

city: Morgantown

first: Nik

last: Kovinich

state: WV

address: 1194 Evansdale Drive

department: Plant and Soil Sciences

country: USA

institute: West Virginia University

library_source: transcriptomic

data_processing:
FastQC software was used to assess quality before further analysis
The reads were then mapped/aligned to Glycine max reference genome (Gmax_275_V2.0.fa, https://phytozome.jgi.doe.gov/pz/portal.html) using STAR
The number of reads per gene was calculated using featureCounts counting only reads which mapped concordantly and —ignore-dup set
Differentially expressed genes (DEGs) were identified using a Negative Binomial Distribution in DESeq2
Genome_build: Wm82.a2 (Glyma 2.0)
Supplementary_files_format_and_content: count data

molecule: polyA RNA

organism: Glycine max

growth_protocol: Harosoy 63 seeds (16 per batch) were sterilized in 30 mL of 70% ethanol, 0.2% triton X (v/v) for 5 min on a mixer wheel, rinsed thrice with sterile water, and imbibed overnight. The imbibate was then discarded to remove growth inhibitors and seeds were transferred to water soaked sterile vermiculite (250 mL in volume) in 500 mL beakers. Beakers were covered with a ring of sterile cheese cloth to permit the passage of light and covered with plastic wrap to ensure aseptic growth. Seedlings were grown at 22 °C under a 16 h photoperiod using cool white T5 fluorescent lights (500 μE m−2 s−1). At the first trifoliate leaf stage (~8 day old), seedling roots were gently wrapped with filter paper saturated with half-strength Murashige and Skoog (MS) medium (pH 5.8) containing vitamins and 1% (w/v) sucrose, and were transferred to their respective abiotic stress treatments.

extract_protocol:
After 9 d of treatment (pH 3.0 medium and control) or 6 d treatment (dehydration and control), seedlings were flash-frozen in liquid nitrogen, lyophilized to dryness, ground to a fine powder and stored at – 80˚C for extraction of metabolites and RNA. Tissue powder was freeze dried again for 1 h, then weighed prior to extractions. Lyophilized tissue was pulverized for 3 min using 5 mm stainless steel grinding balls in a MM400 mixer mill (Retsch, Newtown, CT, USA) at frequency of 30/s. Total RNAs were extracted the using the Spectrum Plant Total RNA Kit.
The mRNA stranded library prep kit from KAPA Biosystems was used to build the libraries

treatment_protocol: Five seedlings per treatment were transferred to a 100 mL beaker containing 50 mL of fresh medium (pH 5.8) for control. Each of the beakers were then placed inside a sterile 500 mL beaker and wrapped with sterile mesh overlaid with plastic wrap to maintain ventilation and aseptic growth conditions. For acidic conditions, seedlings were transferred to beakers containing pH 3.0 medium. For dehydration, the medium-saturated germination paper was allowed to dry gradually in the 100 mL beaker containing no medium.

tag: cultivar

value: Harosoy 63

tag: medium ph

tag: days after treatment

tag: dehydration

value: No

tag: glyceollins elicitation

value: Yes

value: Whole seedling


title: 33_pH3-H63-Seedling-9dat-3

value: Increased


title: Dolly_third_passage

type: genomic

city: lille

first: frédéric

last: leprêtre

address: Boulevard du Pr Leclercq

department: CHU of Lille, CBP

country: France

institute: Lille University

description: new canine model of melanoma

hyb_protocol: Scanned on an Agilent G2505C scanner and Agilent-022060_D_F_20111015 whole canine genome microarrays 4x180K (Probe Name version).

scan_protocol: Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1).

data_processing: Agilent Feature Extraction Software (v 10.7.3.1) was used for background subtraction and LOWESS normalization.   Normalized log10 ratios in sample data tables were obtained with the FE default protocol.

molecule: genomic DNA

organism: Canis lupus familiaris

label_protocol: probe labeling used Agilent Technologies Genomic DNA Enzymatic Labeling Kit. Labelling was performed following manufacturer's instructions.CA).

growth_protocol: RPMI 1640 glutamax growth medium supplemented with 10% heat inactivated fetal calf serum (FCS) and antibiotics (Penicillin 100 U/ mL and Streptomycin 0.1 mg/ mL)

extract_protocol: DNA extraction was performed using the Qiagen DNeasy Kit according to the manufacturer's instructions

treatment_protocol: Surgically removed oral CMM tissue sample were digested mechanically and enzymatically using 2mg/ mL type II collagenase (Gibco®) for two hours at 37°C

tag: sample type

value: third passage

value: oral malignant melanoma

tag: patient id

value: Dolly

source_name: self blood DNA

value: blood


title: Tania_primary_lineage

description: new canine model of melanoma

value: primary lineage

value: Tania


title: PC3_RNA-Seq_siOC2_Hypoxia_siRNA1

city: TORONTO

first: Haiyang

last: Guo

state: Ontario

address: 101 College Street, 11-601D, TMDT, 11-601D, TMDT

department: Princess Margaret Cancer Centre

country: Canada

institute: UNIV HEALTH NETWORK

description:
PC3_RNA-Seq_siOC2_Hypoxia_siRNA1
PC3_RNA-seq_RPKM_OC2_Knockdown.txt
H-SIOC2-1

data_processing:
The trimmed reads were aligned to human genome hg19 with STAR (version 2.4.2a) and gene expression was then quantified using the reads per kilobase per million mapped reads (RPKM) method by Cufflinks (version 2.1.1) with GENCODE v24 GRCh37 GTF file
Genome_build: hg19
Supplementary_files_format_and_content: txt files contain the gene expression matrices

organism: Homo sapiens

source_name: PC3 cells

growth_protocol: LNCaP and PC3 cell lines were obtained from the American Type Culture Collection (ATCC). All prostate cancer cell lines were cultured as recommended by ATCC. No mycoplasma contamination was detected in these cell lines using MycoAlert™ Mycoplasma Detection Kit (LT07-118, Lonza).

extract_protocol:
Total RNA of PC3 and LNCaP cells was extracted using RNeasy Mini Kit (74106, Qiagen) following manufacturer’s procedure.
Whole transcriptome sequencing libraries were prepared using TruSeq® Stranded mRNA Library Prep Kit (RS-122-2101, Illumina, San Diego, CA, USA) by following the manufacturer’s instructions.

treatment_protocol: For hypoxia treatment, cells were incubated in hypoxia chamber at 0.2% O2.

tag: tumor type

value: prostate cancer

tag: cell line

tag: protocol


title: New_pH3-H63-Seedling-9dat-4


title: ALY KD PolyA+

country: China

value: polyA+ RNA


title: GroupC_infection_stage3_A2

city: Townsville

first: David

middle: John

last: Miller

state: Queensland

address: 1 James Cook Drive, Douglas

department: Molecular and Cell Biology

country: Australia

institute: James Cook University

description:
sample name in processed data file:
A2R3

data_processing:
Illumina read were mapped onto Ricordea yuma transcriptome (PRJNA313487) using BOWTIE v2.1.0.
Differential gene expression was inferred by mapping counts using the EdgeR package
Gene ontology analysis was conducted by using GOseq v1.16.1
Genome_build: TSA: Ricordea yuma, transcriptome shotgun assembly (GenBank: GELN00000000.1)
Supplementary_files_format_and_content: text file with counts.

molecule: total RNA

organism: Ricordea yuma

source_name: corallimorph tissue

extract_protocol:
Total RNA was extracted using TRIZOL reagent
cDNA libraries were prepared using NEBNext Ultra RNA Directional Library Prep Kit for Illumina

value: corallimorph tissue

tag: infection status

tag: developmental stage

value: adult

tag: group

value: groupC


title: LUC KD PolyA+

value: HeLa cell

value: uterine cervix

value: control


title: GroupC_infection_stage1_A2

description:
sample name in processed data file:
A2R1


title: LUC KD PolyA-

value: polyA- RNA


title: PC3_RNA-Seq_siOC2_Normoxia_siRNA1

description:
PC3_RNA-Seq_siOC2_Normoxia_siRNA1
PC3_RNA-seq_RPKM_OC2_Knockdown.txt
N-SIOC2-1


title: 25_Cont-H63-Seedling-6dat-1

value: Harosoy 63

value: No

value: Yes

value: Whole seedling


title: Control_infection_stage3_A1

description:
sample name in processed data file:
A1R3


title: 32_pH3-H63-Seedling-9dat-2

value: Increased


title: GroupC_infection_stage2_A2

description:
sample name in processed data file:
A2R2


title: 35_Cont-H63-Seedling-9dat-2


title: 34_Cont-H63-Seedling-9dat-1


title: Control_infection_stage2_A1

description:
sample name in processed data file:
A1R2


title: Control_infection_stage1_A1

description:
sample name in processed data file:
A1R1


title: 36_Cont-H63-Seedling-9dat-3


title: GroupC_infection_stage3_E1

description:
sample name in processed data file:
E1R3


title: MM at diagnosis, sample 30

type: RNA

city: MILAN

first: Luca

last: Agnelli

address: Venezian 1

department: Department of Advanced Diagnostics

country: Italy

institute: IRCCS Istituto Nazionale dei Tumori

hyb_protocol: Byotin-labeled RNA samples were hybridized for 16 hours at 48°C on GeneChip miRNA 3.0 array, according to the standard manifacturer's protocol, using the GeneChip Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.

scan_protocol: Scanning of the arrays was performed using the GeneChip Scanner 7G.

data_processing: Raw data were extracted from CEL files and then normalized using robust multi-array average (RMA) procedure in the affy package for Bioconductor and the miRbase Release 21 annotations included in the corresponding cdf definition files available at the University of Michigan Brainarray portal at http://brainarray.mbni.med.umich.edu/Brainarray/Database/CustomCDF/21.0.0/version.html). Annotations were referred to miRBase Release 21 definition.

label: biotin

source_name: Highly purified PCs from MM patient

label_protocol: 500 ng of total RNA were converted to biotin-labeled RNA using the FlashTag Biotin HSR Labeling kit, according to Affimetrix protocol

extract_protocol: Trizol extraction of total RNA was performed according to the manufacturer's instructions (Gibco BRL).

treatment_protocol: Plasma cells were purified from bone marrow samples using CD138 immunomagnetic microbeads according to the manufacturer's instructions (MidiMACS system, Miltenyi Biotec); the purity of the positively selected PCs was assessed by morphology and flow cytometry and was > 90% in all cases.

value: Bone marrow plasma cells


title: MM at diagnosis, sample 29


title: MM at diagnosis, sample 28

description: hyperdiploid karyotype


title: MM at diagnosis, sample 27


title: MM at diagnosis, sample 26

description: MAF translocation


title: MM at diagnosis, sample 25


title: Ecrg4 hetero2

city: Sapporo

first: Toru

last: Kondo

address: Kita-15, Nishi-7, Kita-ku,

department: Institute for Genetic Medicine

country: Japan

institute: Hokkaido University

hyb_protocol: Hybridized for 16 h at 37 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..

scan_protocol: 3D-Gene Scanner ((Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction(Toray Industries Inc., Tokyo, Japan).

data_processing: The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95% confidence intervals.

organism: Mus musculus

source_name: mouse NSC

label_protocol: Amino Allyl aRNA was synthesis by Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion#1753). CyeDye Coupling and fragmentation were performed as the protocol supplied by TORAY Industries, Inc..

growth_protocol: These NSCs were cultured in the NSC-specific medium that consists of DMEM/F12. chemicals, bFGF, EGF and heparin.

extract_protocol: Total RNA was prepared using a RNeasy Mini Kit (QIAGEN).

treatment_protocol: No treatment

tag: strain

value: brain

tag: age

tag: cell type

value: NSC

tag: genotype

value: Ecrg4 hetero


title: Ecrg4 hetero1

tag: tissue


title: MM at diagnosis, sample 24


title: Ecrg4 homo2

value: Ecrg4 homo


title: Ecrg4 homo1


title: RT112 - lipo#3

city: paris

first: Elodie

last: chapeaublanc

address: 26 rue d'ulm

department: UMR 144

institute: Institut Curie

hyb_protocol: Affymetrix microarrays were hybridized with 10µg of biotinylated cRNA according the supplier recommendations. Microarrays were washed and stained using FS450 fluidics upon a protocol provided by Affymetrix, corresponding to the U133plus 2.0 arrays

scan_protocol: Microarrays were scanned using a 7G, Affymetrix GCS3000 scanner, raw data were acquired with Affymetrix Genechip Command Console.

data_processing: Signal reconstruction was performed with the RMA algorithm as implemented in Bioconductor affy package on R statistical software using the Dai custom CDF package: HGU133Plus2_Hs_ENTREZG Brainarray Custom CDF EntrezG version 18

source_name: human bladder cancer cells

label_protocol: 100ng of total RNA were amplified to produced biotinylated complementary RNAs, as recommended by Affymetrix.

growth_protocol: The bladder cancer cells,MGH-U3 and UM-UC-5, have been culture in DMEM 10%fetal bobine serum and RT112 were cultured in RPMI 10%fetal bobine serum

extract_protocol: mRNAs were extracted and purified using RNeasy Mini kits (Qiagen).

treatment_protocol: MGH-U3, RT112 and UM-UC-5 cells were transfected for 40h either with siRNA TYRO3#1 (ThermoFisher Scientific, 51331 ID:802) siRNA TYRO3#2 (ThermoFisher Scientific,51331 ID:800 ) or siRNA TYRO3#3 (Qiagen, SI00087087) using LipofectaminRNAimax (Invitrogen) according to the manufacturer's instructions (reverse protocol)

tag: transfection


title: RT112 - lipo#2


title: RT112 - siTYRO#3

value: siRNA TYRO3


title: MGHU3 - siTYRO#3


title: RT112 - siTYRO#2


title: 2015_9_HG2

city: Fukuoka

first: Akiko

last: Satake

state: Fukuoka

address: 744 Motooka, Nishi-ku

department: Department of Biology Faculty of Sciences

institute: Kyushu University

description: For each tree, we defined "year of floral induction" as the year before the year when flowering was confirmed.

hyb_protocol: cRNA was hybridized to a 60K 60-mer oligomicroarray (SurePrint G3 Custom Gene Expression 8x60K; Agilent Technologies) according to the manufacturer's instructions.

scan_protocol: The hybridized microarray slides were scanned using an Agilent scanner. The relative hybridization intensities and background hybridization values were calculated using Feature Extraction Software version 9.5.1.1 (Agilent Technologies).

data_processing: The scanned images were analyzed with Feature Extraction Software 9.5.1.1 (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. The raw signal intensities and flags for each probe were calculated from the hybridization intensities and spot information according to the procedures recommended by Agilent Technologies using the Flag criteria in the GeneSpring Software. The raw signal intensities of all samples were  normalized by the quantile algorithm with the Bioconductor.

organism: Fagus crenata

source_name: leaf and bud

label_protocol: cRNA was amplified and labelled using a Low input Quick Amp Labelling Kit (Agilent Technologies).

extract_protocol: Total RNA from leaf and bud samples was extracted using hexadecyltrimethylammonium bromide (CTAB) buffer (3% CTAB, 1.4 M NaCl, 20 mM EDTA, 100 mM Tris–HCl pH 8.0, 0.2% b-mercaptoethanol) and was purified using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany)

tag: year

tag: month

tag: individual id

value: leaf and bud

tag: status of floral induction

value: year of floral induction


title: 2017_5_945


title: 2015_6_HG2


title: 2017_5_944


title: MM at diagnosis, sample 23

description: hyperdiploid karyotype


title: RT112 - siTYRO#1


title: 2016_9_HG3

value: year of no floral induction


title: Ecrg4 wt2

value: Ecrg4 wt


title: 2015_10_HG2


title: MM at diagnosis, sample 22

description: non-hyperdiploid karyotype, no major IgH (14q32) chromosomal translocations


title: RT112 - lipo#1

value: control


title: Ecrg4 wt1


title: GroupD_infection_stage1_B2

description:
sample name in processed data file:
B2R1

value: corallimorph tissue

value: adult

value: groupD


title: 2015_7_HG2


title: 2016_8_HG3


title: 2016_10_HG3


title: 2016_7_HG3


title: MGHU3 - siTYRO#2


title: Mu2: P41nmt1-rpb7+_rep2

city: Bangalore

first: Genotypic

last: technology

state: Karnataka

address: 259, Apoorva 4th cross,80 feet Road,RMV 2ND STAGE

country: India

institute: Genotypic Technology

description: Gene expression in low levels of rpb7+

hyb_protocol: The labeled cRNA samples were hybridized on to a Whole Genome S pombe 8x15k (AMADID: 067936). 600ng of cy3 labeled samples were fragmented and hybridized.  Fragmentation of labeled cRNA and hybridization were done using the Gene Expression Hybridization kit of Agilent (Part Number 5190-0404). Hybridization was carried out in Agilent’s Surehyb Chambers at 65º C for 16 hours. The hybridized slides were washed using Agilent Gene Expression wash buffers (Part No: 5188-5327)

scan_protocol: Slides were scanned using the Agilent Microarray Scanner G2505C at 3 micron resolution.

data_processing: Images were quantified using Feature Extraction Software ( Agilent). Feature extracted raw data was analyzed using GeneSpring GX software from Agilent. Normalization of the data was done in GeneSpring GX using the percentile shift method.

organism: Schizosaccharomyces pombe

source_name: Total RNA, P41nmt1-rpb7+ strain, Biological Replicate2

label_protocol: The samples were labeled using Agilent Quick-Amp labeling kit (Part number: 5190-0442). 500ng each of total RNA were reverse transcribed at 40°C using oligo dT primer tagged to a T7 polymerase promoter and converted to double stranded cDNA. Synthesized double stranded cDNA were used as template for cRNA generation. cRNA was generated by in vitro transcription and the dye Cy3 CTP(Agilent) was incorporated during this step. The cDNA synthesis and in vitro transcription steps were carried out at 40°C. Labeled cRNA was cleaned up using Qiagen RNeasy columns (Qiagen, Cat No: 74106) and quality assessed for yields and specific activity using the Nanodrop ND-1000.

growth_protocol: Strains were grown overnight in EMM media containing 15μM thiamine and sub-cultured in the same fresh media and allowed to grow for further 12 hours and then cells were pelleted.

extract_protocol: RNA extraction was performed by standard hot phenol method as described by Lyne et al., 2003.

value: P41nmt1-rpb7+ in 972h- background


title: 2015_10_HG3


title: pep2_rep3

city: Köln

first: Maria

last: Albani

address: Carl-von-Linné-Weg 10

department: Botanical institute

country: Germany

institute: University of Cologne

data_processing:
Filtered for per base Phred score greater than 25
Mapping of RNA-seq reads done by TopHat with default parameters
Expression Value was estimated by Cuffdiff considering all replicates
Differential gene expression analysis performed by Cuffdiff which uses t-test and pval calculation for each comparison
Adjustment of pval was done by Cuffdiff using  Benjamini-Hochberg method
Further analysis, like filtering on the basis of logFC and padj were performed by various application of Linux
Genome_build: Arabis alpina V4 genome
Supplementary_files_format_and_content: excel files include RPKM values for each Sample

organism: Arabis alpina

source_name: 3 weeks old plants

growth_protocol: Plants are grown for 3 weeks at long day 16h light : 8 h dark.

extract_protocol:
RNA was isolated using RNeasy Plant RNA isolation kit (Qiagen) according to manufacturer's instructions
The libraries were prepared from 1 µg total RNA with RNA integrity number >8 (Bioanalyzer; Agilent) using TruSeq type RNA kit (Illumina) at the Cologne Genome Centre and sequenced as 100-bp single-end reads on HiSeq2500 (Illumina) instrument.

value: dissected shoot apices

tag: cultivar

value: Pajares


title: MM at diagnosis, sample 20

description: hyperdiploid karyotype

value: Bone marrow plasma cells


title: pep2_rep2


title: MGHU3 - siTYRO#1


title: MM at diagnosis, sample 21

description: hyperdiploid karyotype


title: pep1-1_rep3


title: 2016_6_HG3


title: MM at diagnosis, sample 19


title: MM at diagnosis, sample 18


title: Mu1: P41nmt1-rpb7+_rep1

description: Gene expression in low levels of rpb7+

source_name: Total RNA, P41nmt1-rpb7+ strain, Biological Replicate1


title: 2015_8_HG3


title: WT1: Wild type_rep1

description: Gene expression in wild type cells

source_name: Total RNA, Wild type strain, Biological Replicate1


title: MGHU3 - lipo#2


title: MM at diagnosis, sample 16


title: WT2: Wild type_rep2

description: Gene expression in wild type cells

source_name: Total RNA, Wild type strain, Biological Replicate2


title: MGHU3 - lipo#1


title: 2015_9_HG3


title: MGHU3 - lipo#3


title: 2015_7_HG3


title: pep2_rep1


title: MM at diagnosis, sample 17


title: UMUC5 - siTYRO#3


title: 2015_6_HG3


title: pep1-1_rep1


title: UMUC5 - siTYRO#2


title: WT_rep3

value: wild-type


title: MM at diagnosis, sample 15

description: MAF translocation


title: MM at diagnosis, sample 14


title: 2016_10_HG2


title: WT_rep1


title: WT_rep2


title: MM at diagnosis, sample 13


title: UMUC5 - siTYRO#1


title: MM at diagnosis, sample 12


title: MM at diagnosis, sample 11

description: hyperdiploid karyotype


title: UMUC5 - lipo#3


title: C2_non_inflamed

city: Oxford

first: Alison

last: Simmons

address: John Radcliffe Hospital/Headley Way

department: The MRC Weatherall Institute of Molecular Medicine

country: United Kingdom

institute: University of Oxford

data_processing:
Basecalling was performed using Illumina Casava software
Samples were demultiplexed using Illumina bcl2fasq software
Sequenced reads were processed, aligned and counts summarised using Cellranger 2.1 pipeline
Genome_build: hg38
Supplementary_files_format_and_content: tab-delimited UMI counts per  10,000 unique molecules detected

growth_protocol: n/a - no samples were cultured

extract_protocol:
After study ethical approval fresh intestinal biopsies were collected during colonoscopy  in those undergoing routine / screening colonoscopy (Healthy) of in those with known ulcerative colitis (UC). Samples were transported on ice and epithelial crypts isolated using a chelation medium (HPGA with 1mM EDTA) and then isolated crypts digested to a single cell solution (TryPLE Express with DNAse) before being processed in 10X chromium as per manufacturers protocol.
Library construction by 3’ prime V2 chemistry Chromium single cell gene expression kit.

tag: subject status

value: Ulcerative colitis

value: adjacent non-inflamed area

value: epithelial cells


title: 2016_10_HG1


title: C3_inflamed

value: inflamed area of colon


title: 2016_9_HG1

value: leaf and bud


title: UMUC5 - lipo#1

tag: cell line


title: 2016_8_HG2


title: MM at diagnosis, sample 9

description: no information about hyperdiploid status, no major IgH (14q32) chromosomal translocations


title: C1_healthy

value: healthy control

value: colonic biopsy


title: 2016_8_HG1

value: year of no floral induction


title: 2016_9_HG2


title: B1_healthy


title: 2016_7_HG1


title: Mycorrhized transgenic MtGras1 RNAi roots rep2

city: Hannover

first: Helge

last: Küster

address: Herrenhäuser Str. 2

department: Institut für Pflanzengenetik

institute: Leibniz Universität Hannover

description: Gene expression data from mycorrhized transgenic MtGras1 RNAi roots

hyb_protocol: Standard hybridization, post-hybridization wash and double-staining as well as scanning was done as specified for GeneChip® Medicago Transcriptome Assays (ThermoFisher Scientific, Schwerte, Germany).

scan_protocol: GeneChips were scanned on an Affymetrix GeneChip scanner 3000 7G.

data_processing: Cel files were analysed using the Expression Console and Transcriptome Analysis Console software (both ThermoFisher Scientific, Schwerte, Germany). Normalization was performed via the Robust Multichip Average algorithm. Intensity values calculated for each probe set were log2-transformed and averaged across all three biological replicates. Expression ratios were evaluated statistically via tools implemented in the Transcriptome Analysis Console.

organism: Medicago truncatula

source_name: Rhizophagus irregularis-colonized Medicago truncatula root fragments

label_protocol: Biotinylated aRNA obtained from 100 ng of total RNA for each sample was fragmented as recommended (GeneChip® Medicago Transcriptome Assay, ThermoFisher Scientific, Schwerte, Germany). The size distribution of the fragmented aRNA was assessed via an Agilent bioanalyzer (Agilent Technologies, Böblingen, Germany) using an RNA 6000 assay.

growth_protocol: Medicago truncatula Gaertn cv Jemalong genotype A17 seeds were surface-sterilized and scarified. Plants were grown in the climate chamber (humidity: 60 %; photosynthetic photon flux: 150 μmol m-2 s-1) at a 16 h light (23 °C) and 8 h dark (18 °C) regime.

extract_protocol: Total RNA was isolated using the RNeasy Micro kit (Qiagen, Hilden, Germany). On-column DNAse I digestion was performed according to the manufacturer’s instructions.

treatment_protocol: Plants were mycorrhized with Rhizophgus irregularis under conditions of phosphate limitation (20 µM phosphate).

value: Root

value: Jemalong A17

value: 54 days


title: MM at diagnosis, sample 8


title: UMUC5 - lipo#2

value: control


title: B2_non_inflamed


title: 2016_7_HG2


title: 2016_6_HG2


title: Mycorrhized transgenic MtGras1 RNAi roots rep1


title: MM at diagnosis, sample 7


title: Mycorrhized transgenic control roots expressing a gusAint reporter gene rep3

description: Gene expression data from mycorrhized transgenic control roots expressing a gusAint reporter gene


title: MM at diagnosis, sample 6

description: hyperdiploid karyotype


title: B3_inflamed


title: Mycorrhized transgenic control roots expressing a gusAint reporter gene rep2


title: 2016_6_HG1


title: 2015_10_HG1

value: year of floral induction


title: MM at diagnosis, sample 5

description: hyperdiploid karyotype


title: A2_non_inflamed


title: MM at diagnosis, sample 3

description: hyperdiploid karyotype


title: Mycorrhized ram1-1 mutant roots rep3

description: Gene expression data from mycorrhized ram1-1 mutant roots

value: 35 days


title: MM at diagnosis, sample 2

description: MAF translocation


title: Mycorrhized transgenic control roots expressing a gusAint reporter gene rep1


title: A3_inflamed


title: A1_healthy


title: 2015_8_HG1


title: Noc 8h Rep2

status: Public on Feb 26 2019

city: Singapore

first: YANG

last: SHEN

address: Genome Institute of Singapore (A*STAR) 60 Biopolis Street, #02-01 Genome

country: Singapore

institute: Genome Institute of Singapore

data_processing:
RNA-seq reads (Illumina HiSeq High Output V3, 1×101bp, single-end) were aligned to the human genome by Tophat 2.1.0. Quality control of single-ended reads was performed by RNA-SeQC (1.1.7). The quality control results were all within acceptable ranges. Cuffdiff (2.2.1) was used to quantify expression and detect differentially expressed genes with gene annotation GTF file downloaded from Ensembl (version GRCh37.75).
Genome_build: hg19
Supplementary_files_format_and_content: bedgraph file generated using Bedtools

source_name: hTERT RPE-1

growth_protocol: hTERT RPE-1 cells were grown in DMEM/F-12 media supplemented with 10% FBS and 1%  penicillin/streptomycin.

extract_protocol:
Total RNA was extracted using RNeasy plus Mini Kit (Qiagen).
RNA libraries were prepared for sequencing using standard Illumina protocols.

treatment_protocol: To enrich for aneuploid cells, RPE-1 cells were first synchronized at G1/S phase transition by first subjected to serum starvation for 24 h (G0/G1 phase), followed by wash-out and treatment with 3 mM thymidine for 24 h (G1/S phase). Three hours after release from thymidine, cells were treated with 100 ng/ml nocodazole for 8 h or 16 h. Nocodazole-arrested mitotic cells were isolated by shake-off, washed in fresh media twice, and replated in fresh media for 24 h to generate aneuploid cells. Synchronized RPE-1 cells without drug treatment serve as control group.

value: Epithelial cells immortalized with hTERT

value: Retina

tag: treatment

value: nocodazole for 8h


title: Noc 16h Rep2

value: nocodazole for 16h


title: 2015_7_HG1


title: 2015_9_HG1


title: Noc 8h Rep1


title: Noc 16h Rep1


title: Mycorrhized ram1-1 mutant roots rep1


title: Mycorrhized wild type control roots rep3

description: Gene expression data from mycorrhized wild type control roots


title: MM at diagnosis, sample 4

value: Bone marrow plasma cells


title: Mycorrhized ram1-1 mutant roots rep2


title: Control Rep1


title: MM at diagnosis, sample 1


title: 2015_6_HG1


title: Control Rep2


title: Mycorrhized wild type control roots rep1


title: Mycorrhized wild type control roots rep2


title: RC2

city: qingdao

first: Hao

last: Xu

state: Shandong

address: 106 Nanjing Road

institute: Yellow Sea fisheries Research Institution

data_processing:
Illumina Casava software used for basecalling.
Index of the reference genome was built using Bowtie v2.2.3 and paired-end clean reads were aligned to the reference genome using TopHat v2.0.12. We selected TopHat as the mapping tool for that TopHat can generate a database of splice junctions based on the gene model annotation file and thus a better mapping result than other non-splice mapping tools.
HTSeq v0.6.1 was used to count the reads numbers mapped to each gene. And then FPKM of each gene was calculated based on the length of the gene and reads count mapped to this gene.
Genome_build: GCF_000523025.1
Supplementary_files_format_and_content: tab-delimited text file include RPKM values for each Sample.

organism: Cynoglossus semilaevis

source_name: Spleen, Kidney, Liver

extract_protocol:
The fish were sampled before injection and 24 hpi, and the liver, spleen, kidney tissues were collected from three fish each group, immediately frozen in liquid nitrogen. mRNA was purified from the mixed 3 µg total RNA using poly-T oligo-attached magnetic beads
cDNA libraries were constructed using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations.

treatment_protocol: The bacteria vibrio harveyi were incubated in medium 2216E at 28 ˚C for 18 hours, collected by centrifugation, and suspended to a bacteria density (OD600=1.0) in phosphate-buffered saline (PBS), and 0.2 mL Vibrio harveyi suspension was injected into the abdominal cavity.

value: resistant family

value: Spleen, Kidney, Liver

value: One year post fertilization

value: Challenged with Vibrio harveyi at 24 h


title: SU2

value: susceptible family

value: Unchallenged with Vibrio harveyi


title: SC1


title: RU1


title: SC3


title: RU3


title: RU2


title: RC3


title: SC2


title: RC1


title: SU3


title: ChIPseq_Pax3_6day_dox_EB_rep2

city: Minneapolis

first: Alessandro

last: Magli

state: MN

address: 2231 6th St SE

department: Medicine

institute: University of Minnesota

description:
processed data file:
mouse_Pax3_6day_ChIP_peaks.bed
ChIP_Pax3_6day_plusdox_02

library_source: genomic

data_processing:
ChIP-seq analysis. Each sample’s reads were aligned to the mouse (mm10) or human (hg38) genome using Bowtie2 followed by removal of PCR duplicates using Samtools. Peak calling was performed using MACS with the following parameters: --bw 300 -p 1e-3. Similar results were obtained by performing peak calling using QESEQ with the following parameters: -s 100 -c 15 -p 0.01. To identify the list of high confidence mouse Pax3 peaks we performed 3 independent Pax3 ChIP-seq experiments and, using the bedtools intersect function, only common regions between 2 experiments were further considered. In addition, the Pax3 peak list was filtered (intersect –v option) for sites overlapping to peaks detected in the uninduced control ChIP-seq and in the mouse (or human) ChIP-seq black-list. Density maps were generated with SeqMiner using the enrichment file enr.sgr produced by QESEQ. Bigwig files for visualization on IGV were generated by converting the wig files obtained from MACS  using the Kent tool Wig-to-BigWig.
Genome_build: mm10
Supplementary_files_format_and_content: ChIP-seq: processed files represent list of Pax3 peaks obtained by removing Encode blacklist and peaks detected in non-induced samples or input

source_name: differentiating mES cells

growth_protocol: Mouse ES cell differentiation was achieved by diluting 40000 cells/ml in EB differentiation medium and incubation in an orbital shaker at 80 RPM. EB differentiation medium: IMDM supplemented with 15% FBS , 1% Penicillin/Streptomycin, 2mM Glutamax, 50 μg/ml Ascorbic acid, 4.5 mM Monothioglycerol. For Serum-free differentiation, FBS was replaced with an equivalent amount of Knock-outTM Serum Replacement (Invitrogen). Trangene induction was achieved by adding doxycycline (Sigma-Aldrich) to day 3 EBs cultures (final concentration 1µg/ml), and then maintained throughout the differentiation protocol by replacing the media (including dox) every two days. At day 5, EBs were disgregated and single cells were incubated for 20 minutes with PDGFRα-PE and FLK1-APC conjugated antibodies. PDGFRα+FLK1- cells were sorted using FACSAriaII (BD biosciences) and replated on gelatin coated dishes using serum- or serum-free EB differentiation media supplemented with 1µg/ml doxycycline and 10ng/ml mouse basic-FGF.

extract_protocol:
ChIP-seq: D4 EBs (1-day induced and control Pax3 mouse cells) were trypsin-treated at 37°C for 1 minute with gentle shacking and reaction was inhibited by adding 10%FBS/PBS. 1-day induced Pax3 NIH3T3 cells and 6-day induced Pax3 mouse cells growing in monolayer were harvested by incubation with trypsin for 1-2 minutes. Human differentiating PAX3-inducible cells were collected at day 6 of differentiation (following 24h dox-mediated induction) by incubation with trypsin for 1-2 minutes. Single cells were washed once with PBS, resuspended in 10%FBS/PBS and supplemented with formaldehyde (final concentration 1%) for crosslinking of protein-DNA complexes (10 minutes at RT) followed by quenching with glycine and staining with PDGFRα-PE antibody. PDGFRα+ cells were sorted using FACSAriaII, snap-frozen in liquid nitrogen and stored at -80°C if not processed immediately. Cell pellets were incubated in lysis buffer LB1 supplemented with protease inhibitors (50mM HEPES KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% NP40, 0.25% Triton X-100 + Complete-mini - Roche) for 10 minutes at +4°C followed by incubation in buffer LB2 supplemented with protease inhibitors (10mM TRIS HCl pH 8, 200mM NaCl, 1mM EDTA, 0.5mM EGTA + Complete-mini - Roche) for 10 minutes at +4°C. Cell pellet was then resuspended in LB3 supplemented with protease inhibitors (10mM TRIS HCl pH 8, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% Sodium Deoxycholate, 0.5% N-lauroylsarcosine + Complete-mini - Roche) and then sonicated. Cells were sonicated with a Branson sonicator at 18% power for 1 minute with intervals of 10 sec ON-10 sec OFF to achieve an average chromatin size of 300bp. After shearing, samples were centrifuged for 10 minutes at 16000g and snap frozen in liquid nitrogen if not processed immediately. For Pax3 ChIP, 25-40µg of chromatin (diluted to 500µl) were precleared for 4h at 4°C with 20µl of BSA-blocked Protein A (or Protein G)-conjugated sepharose beads (GE healthcare). Samples were supplemented with 1/10 volume of 10% Triton X-100 and incubated overnight with anti-Pax3 (Santa Cruz Biotechnology sc-34926) antibody. Immune complexes were recovered by incubation with 20µl of BSA-blocked Protein G-conjugated sepharose beads for 4h at 4°C and then washed 5 times with RIPA wash buffer (50mM HEPES KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% NP40, 0.25% Triton X-100, 0.7% Sodium Deoxycholate) and one time with TEN buffer (10mM TRIS HCl pH 8, 1mM EDTA, 50mM NaCl). Immunoprecipitated chromatin was recovered by incubating beads with 200µl of Elution buffer (50mM TRIS HCl pH 8, 10mM EDTA, 1% Sodium Dodecyl Sulfate) for 20 minutes at 65°C. Chromatin from IP and Input (equivalent to 1% of starting material) was reverse crosslinked overnight at 65°C, then diluted 1:1 with TE (10mM TRIS HCl pH 8, 1mM EDTA) supplemented with 4µl of RNaseA 20mg/ml and incubated for 2 hours at 37°C followed by Proteinase K treatment (4µl of 20mg/ml stock for each sample) for 30 minutes at 55°C. DNA was purified by Phenol-chloroform-isoamyl alcol extraction (twice) followed by chloroform extraction, then supplemented with 1/10 of volume of 3M Sodium Acetate pH 5 and 1.5µl of Glycogen and precipitated with 2 volumes of 100% Ethanol at -80°C for >1 hour. Followed 30 minutes centrifuge at 16000g, pellet were washed with 75% ethanol, air dried and dissolved in 45µl H2O.
ChIP-seq libraries were generated following a gel-free protocol using AMPure XP beads (Beckman Coulter) for all the purification and size selection steps. 10ng or less of DNA were end repaired using End-it DNA end repair (Epicentre), then A-tailed using Klenow Fragment (3'→5' exo-  NEB) followed by adapter-barcode ligation using T4 DNA ligase (Enzymatics). Illumina compatible adapter-barcodes were purchased from BIOO scientific. After ligation, DNAs were negatively size selected using 0.5x Ampure XP beads and unbound DNAs were positively size selected by adding 0.4x Ampure XP beads (this step allows for retention of DNA fragments ranging 200-500bp). Libraries were amplified using Phusion High Fidelity PCR master mix 2x (NEB) with a 16 cycles program. Libraries from iPax3 NIH3T3 cells were generated using the NEBNext DNA library prep kit (NEB).

value: differentiating mES cells

tag: differentiation protocol

value: serum

tag: time

value: day9 of differentiation

tag: purification

value: PDGFRα+FLK1- sorted

tag: doxycycline-treatment


title: ChIPseq_Pax3_nodox_EB_rep3

description:
processed data file:
mouse_Pax3_1day_ChIP_peaks.bed
ChIP_Pax3_1day_nodox_03

value: day4 of differentiation

value: not treated


title: Input_6day_dox_rep1

description:
processed data file:
mouse_Pax3_1day_ChIP_peaks.bed
Input_6day_plusdox_01


title: Input_6day_dox_rep2

description:
processed data file:
mouse_Pax3_1day_ChIP_peaks.bed
Input_6day_plusdox_02


title: ChIPseq_Pax3_1day_dox_EB_rep2

description:
processed data file:
mouse_Pax3_1day_ChIP_peaks.bed
ChIP_Pax3_1day_plusdox_02


title: ChIPseq_Pax3_1day_dox_EB_rep3

description:
processed data file:
mouse_Pax3_1day_ChIP_peaks.bed
ChIP_Pax3_1day_plusdox_03


title: ATACseq_serum_Pax3_rep3

description: ATAC-seq

data_processing:
ATAC-seq analysis. Reads were aligned to the mouse genome (mm10) using Bowtie2 with the following parameters: -q -I 38 -X 2000 --local --dovetail --no-mixed --no-discordant. Aligned reads were filtered for PCR duplicates using Samtools and then processes with MACS 1.4 with the following parameters: --nomodel --nolambda --keep-dup all --call-subpeaks -p 1e-4 -w –S. Bigwig files for visualization on IGV were generated by converting the wig files obtained from MACS using the Kent tool Wig-to-BigWig. ATAC-seq reads overlapping to Pax3 peaks were retrieved using the R Bioconductor package and then used to generate density maps with Seqminer. To identify differentially accessible peaks, we used the BEDTools intersect function: the common regions (-f 0.5 -F 0.5 -e options) for the dox-induced samples were intersected (-v -f 0.2 options) with the combined list of control peaks (from non-induced cells) and the resulting list was then compared (-f 0.5 -F 0.5 –e options) with the peak list detected in PDGFRα+FLK1- cells from E9.5 embryos.
Genome_build: mm10
Supplementary_files_format_and_content: ATAC-seq: processed files contain the list of peaks identified by MACS in each replicate

extract_protocol: ATAC-seq: 50,000 freshly sorted cells from differentiated ES cultures, iPax3 NIH3T3 fibroblasts and single embryos were washed with 200 µl of cold PBS then resuspended in 100 µl of cold lysis buffer (10mM Tris-HCl pH 7.4, 10mM NaCl, 3mM MgCl2, 0.1% IGEPAL CA-630), spun at 500 g for 10 minutes at 4°C and resuspended in 50 µl of the transposition reaction mix. Transposition occurred at 37°C for 30 minutes, after which transposed DNA was purified using a Qiagen MinElute Kit and eluted in 10µl Elution Buffer. Final libraries were generated by primer extension of the transposed DNA followed by PCR amplification using Illumina-compatible adapter-barcodes.


title: ChIPseq_Pax3_nodox_EB_rep2

description:
processed data file:
mouse_Pax3_1day_ChIP_peaks.bed
ChIP_Pax3_1day_nodox_02


title: Input_nodox_rep2

description:
processed data file:
mouse_Pax3_1day_ChIP_peaks.bed
Input_nodox_02


title: ChIPseq_Pax3_6day_dox_EB_rep1

description:
processed data file:
mouse_Pax3_6day_ChIP_peaks.bed
ChIP_Pax3_6day_plusdox_01


title: ChIPseq_Pax3_nodox_EB_rep1

description:
processed data file:
mouse_Pax3_1day_ChIP_peaks.bed
ChIP_Pax3_1day_nodox_01


title: ATACseq_serum_ctrl_rep3

description: ATAC-seq


title: 2015_8_HG2

description: For each tree, we defined "year of floral induction" as the year before the year when flowering was confirmed.

value: leaf and bud

value: year of floral induction


title: ATACseq_serum_free_ctrl_rep1

description:
processed data file:
Ctrl_KSR_A_ATAC_peaks.bed
serum_free_ctrl_01

value: serum-free

value: unsorted


title: ATACseq_NIH3T3_ctrl_rep2

description:
processed data file:
3T3_Pax3_nodox_C_ATAC_peaks.bed
3T3_Pax3_nodox_C

source_name: murine fibroblasts

value: murine fibroblasts

value: not applicable


title: ATACseq_serum_free_ctrl_rep2

description:
processed data file:
Ctrl_KSR_B_ATAC_peaks.bed
serum_free_ctrl_02


title: ATACseq_serum_Pax3_rep1

description:
processed data file:
Pax3_EBM_plus_B_ATAC_peaks.bed
serum_Pax3_01


title: ATACseq_serum_6day_Pax3_rep2

description:
processed data file:
myog_prog_B_ATAC_peaks.bed
6_day_serum_Pax3_02


title: ATACseq_serum_free_Pax3_rep2

description:
processed data file:
Pax3_KSR_B_ATAC_peaks.bed
serum_free_Pax3_02


title: ATACseq_serum_free_Myf5_rep2

description:
processed data file:
Myf5_KSR_B_ATAC_peaks.bed
serum_free_Myf5_02


title: ATACseq_NIH3T3_ctrl_rep1

description:
processed data file:
3T3_Pax3_nodox_A_ATAC_peaks.bed
3T3_Pax3_nodox_A


title: ATACseq_serum_ctrl_rep2

description:
processed data file:
Ctrl_EBM_no_C_ATAC_peaks.bed
serum_ctrl_02


title: Input_nodox_rep1

description:
processed data file:
NIH3T3_Pax3_1day_ChIP_peaks.bed
Input_nodox_01


title: ATACseq_NIH3T3_Pax3_rep1

description:
processed data file:
3T3_Pax3_plusdox_A_ATAC_peaks.bed
3T3_Pax3_plusdox_A


title: ATACseq_serum_free_Myf5_rep1

description:
processed data file:
Myf5_KSR_A_ATAC_peaks.bed
serum_free_Myf5_01


title: ATACseq_serum_ctrl_rep1

description:
processed data file:
Ctrl_EBM_no_B_ATAC_peaks.bed
serum_ctrl_01


title: Input_1day_dox_rep2

description:
processed data file:
mouse_Pax3_1day_ChIP_peaks.bed
Input_plusdox_02


title: SU1

tag: group

value: Spleen, Kidney, Liver

tag: age


title: ATACseq_serum_free_Pax3_rep1

description:
processed data file:
Pax3_KSR_A_ATAC_peaks.bed
serum_free_Pax3_01


title: ATACseq_NIH3T3_Pax3_rep2

description:
processed data file:
3T3_Pax3_plusdox_C_ATAC_peaks.bed
3T3_Pax3_plusdox_C


title: ChIPseq_Pax3_1day_dox_EB_rep1

description:
processed data file:
mouse_Pax3_1day_ChIP_peaks.bed
ChIP_Pax3_1day_plusdox_01


title: ATACseq_serum_free_Msgn1_rep2

description:
processed data file:
Msgn1_KSR_B_ATAC_peaks.bed
serum_free_Msgn1_02


title: ATACseq_serum_free_Msgn1_rep1

description:
processed data file:
Msgn1_KSR_A_ATAC_peaks.bed
serum_free_Msgn1_01


title: ATACseq_serum_Myf5_rep2

description:
processed data file:
Myf5_EBM_B_ATAC_peaks.bed
serum_Myf5_02


title: Input_1day_dox_rep1

description:
processed data file:
NIH3T3_Pax3_1day_ChIP_peaks.bed
Input_plusdox_01


title: ATACseq_serum_Msgn1_rep2

description:
processed data file:
Msgn1_EBM_B_ATAC_peaks.bed
serum_Msgn1_02


title: ATACseq_Pax3_ko_embryo_rep3

description:
processed data file:
PF_7118-5_Pax3_KO_ATAC_peaks.bed
E9_5_PF_Pax3_ko_03

source_name: E9.5 embryo

value: E9.5 embryo

value: 9.5 days post coitum


title: ATACseq_Pax3_wt_embryo_rep3

description:
processed data file:
PF_7269-7_Pax3_WT_ATAC_peaks.bed
E9_5_PF_Pax3_wt_03


title: ATACseq_Pax3_ko_embryo_rep2

description:
processed data file:
PF_7118-3_Pax3_KO_ATAC_peaks.bed
E9_5_PF_Pax3_ko_02


title: ATACseq_Pax3_wt_embryo_rep2

description:
processed data file:
PF_7118-4_Pax3_WT_ATAC_peaks.bed
E9_5_PF_Pax3_wt_02


title: ATACseq_Pax3_wt_embryo_rep1

description:
processed data file:
PF_6844-3_Pax3_WT_ATAC_peaks.bed
E9_5_PF_Pax3_wt_01


title: rnaseq_preleptotene_Stra8-het_lowSTRA8_rep2

city: Cambridge

first: Mina

middle: L

last: Kojima

state: MA

address: 455 Main Street

institute: Whitehead Institute

description: sorted_male_preleptotenes_STRA8-het_lowSTRA8_RNAseq_2

data_processing:
For ChIP-seq: FASTX Toolkit v0.0.14 was used to filter out low-quality sequencing reads and to trim reads to 40 bp.
For ChIP-seq: reads were aligned to the mm10 mouse genome using bowtie1 v1.2.0 allowing one mismatch per read.
For ChIP-seq: peaks were called using MACS2 v2.1.1.20160309 by comparing ChIP samples with corresponding input chromatin libraries.
For ChIP-seq: SAMtools v1.3.1 was used to generate BAM files for all mapped reads with duplicate reads removed.
For ChIP-seq: BigWig files for input-subtracted normalized ChIP-seq signal were generated using bamcompare v2.5.3 from deepTools.
For RNA-seq: for each sample, raw reads from two sequencing lanes were filtered for quality, trimmed to 40bp, and merged.
For RNA-seq: expression levels of all transcripts were estimated using kallisto v0.43.0 with sequence-bias correction. We used the set of transcripts in the basic GENCODE M15 annotation (Ensembl 90).
For RNA-seq: expression levels for protein-coding transcripts on reference chromosomes (mm10) were extracted, summed by gene, and re-normalized to transcript-per-million (TPM) units.
For RNA-seq: normalized counts per gene were obtained using DESeq2 v1.18.1 with default parameters. The estimated count values from kallisto were supplied to DESeq2.
Genome_build: mm10
Supplementary_files_format_and_content: bigWig files represent normalized input-subtracted ChIP signal. Input and ChIP libraries were normalized using RPKM.
Supplementary_files_format_and_content: bed files represent the summits of the "peaks" of heighted read density that were identified in each ChIP sample compared to its corresponding input chromatin sample.
Supplementary_files_format_and_content: kallisto abundance files for RNA-seq represent estimated abundances for every transcript.
Supplementary_files_format_and_content: TPM value files include normalized expression values per gene for all protein-coding transcripts on reference chromosomes (mm10).
Supplementary_files_format_and_content: DESeq normalized count files represent the normalized counts obtained from supplying the estimated count values from kallisto to DESeq2.

source_name: preleptotene spermatocytes from postnatal testis

extract_protocol: Sequencing libraries were prepared using commercial kits.

treatment_protocol: Mouse testes enriched for meiosis-initiating germ cells were obtained by synchronizing spermatogenesis in vivo by the WIN18,446/RA protocol. Male mice were treated with WIN18,446 daily from postnatal day (P) 2 to P8, and they were treated with retinoic acid on P9. Testes were collected ~6.75 days after the RA injection. A small portion of the testis was reserved for histology to confirm successful synchronization and the enrichment of preleptotene cells (germ cells initiating meiosis).

tag: developmental stage

value: preleptotene stage

tag: strain

tag: sorting

value: sorted preleptotene germ cells

tag: genotype

value: Stra8 heterozygous

tag: Sex

value: male

tag: sample type

value: RNA-seq

tag: library prep kit

value: TruSeq Stranded mRNA kit (Illumina) with poly-A selection

tag: extract protocol

value: Extract protocol:Preleptotene cells (cells initiating meiosis) were sorted from testes with synchronized spermatogenesis. RNA extraction was performed using the QIAshredder homogenizer and RNeasy Plus Micro Kit.


title: rnaseq_preleptotene_Stra8-het_highSTRA8_rep2

description: sorted_male_preleptotenes_STRA8-het_highSTRA8_RNAseq_2

tag: tissue

value: preleptotene spermatocytes from postnatal testis


title: rnaseq_preleptotene_Stra8-KO_rep1

description: sorted_male_preleptotenes_STRA8-KO_RNAseq_1

value: Stra8 knock-out


title: chipseq_FF_rep3_chip

description: synchronizedtestis_Stra8-FLAGFLAG_FLAGchipseq_3_chip

source_name: postnatal testis

value: postnatal testis

value: developmental synchronization to enrich for preleptotenes

value: no sorting

value: Stra8 FLAG/FLAG

value: ChIP-seq with FLAG M2 antibody (F1804, Sigma)

value: Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences)

value: ChIP protocol:Samples were fixed and sonicated, and the chromatin was immunoprecipitated with the FLAG antibody. For each ChIP sample, a small fraction of the sonicated chromatin was saved as input chromatin control. Crosslinking was reversed, samples were treated with RNaseA and proteinase K, and the DNA was purified.


title: rnaseq_preleptotene_Stra8-KO_rep3

description: sorted_male_preleptotenes_STRA8-KO_RNAseq_3


title: rnaseq_preleptotene_Stra8-het_lowSTRA8_rep1

description: sorted_male_preleptotenes_STRA8-het_lowSTRA8_RNAseq_1


title: rnaseq_preleptotene_Stra8-KO_rep2

description: sorted_male_preleptotenes_STRA8-KO_RNAseq_2


title: rnaseq_preleptotene_Stra8-het_highSTRA8_rep1

description: sorted_male_preleptotenes_STRA8-het_highSTRA8_RNAseq_1


title: chipseq_FF_rep2_input

description: synchronizedtestis_Stra8-FLAGFLAG_FLAGchipseq_2_input

value: Input chromatin control for ChIP-seq sample

value: TruSeq ChIP Sample Preparation Kit (Illumina)


title: chipseq_WT_rep3_chip

description: synchronizedtestis_Stra8-wildtype_FLAGchipseq_3_chip

value: wild-type


title: chipseq_FF_rep1_chip

description: synchronizedtestis_Stra8-FLAGFLAG_FLAGchipseq_1_chip


title: ATACseq_serum_Myf5_rep1

description:
processed data file:
Myf5_EBM_A_ATAC_peaks.bed
serum_Myf5_01

value: differentiating mES cells

value: serum

value: day4 of differentiation

value: PDGFRα+FLK1- sorted


title: chipseq_FF_rep1_input

description: synchronizedtestis_Stra8-FLAGFLAG_FLAGchipseq_1_input

value: male


title: chipseq_WT_rep3_input

description: synchronizedtestis_Stra8-wildtype_FLAGchipseq_3_input


title: ATACseq_serum_Msgn1_rep1

description:
processed data file:
Msgn1_EBM_A_ATAC_peaks.bed
serum_Msgn1_01


title: chipseq_WT_rep2_chip

description: synchronizedtestis_Stra8-wildtype_FLAGchipseq_2_chip


title: chipseq_WT_rep2_input

description: synchronizedtestis_Stra8-wildtype_FLAGchipseq_2_input


title: ATACseq_Pax3_ko_embryo_rep1

description:
processed data file:
PF_6844-6_Pax3_KO_ATAC_peaks.bed
E9_5_PF_Pax3_ko_01

value: E9.5 embryo

value: not applicable

value: 9.5 days post coitum


title: chipseq_WT_rep1_input

description: synchronizedtestis_Stra8-wildtype_FLAGchipseq_1_input

value: postnatal testis

value: developmental synchronization to enrich for preleptotenes


title: chipseq_FF_rep3_input

description: synchronizedtestis_Stra8-FLAGFLAG_FLAGchipseq_3_input

value: no sorting

value: Stra8 FLAG/FLAG


title: chipseq_WT_rep1_chip

description: synchronizedtestis_Stra8-wildtype_FLAGchipseq_1_chip

value: ChIP-seq with FLAG M2 antibody (F1804, Sigma)


title: shGRP78 (18)

city: Miami

first: Patricia

last: Dauer

state: FL

address: 1550 NW 10th Ave

department: Surgery

institute: University of Miami

data_processing:
Sequenced on a 75 bp pair-end run using NextSeq 500 High Output Kit 150-cycle; 400 M flow cell
6 barcoded libraries were sequenced across 4 lanes
864.33 M pass filter paired-end reads (432.17 M clusters) were generated
44.8 M paired-end reads (22.4 M clusters) were unindexed
sequence data was processed through PartekFlow RNAseq pipeline (Partek Inc., St. Louis, MO) as follows- A pre-alignment QA/QC was performed and bases with Q>30 were retained for analysis.
Bowtie2 was used to filter out non-human DNA, mtDNA and rDNA from the samples and STAR 2.5.3 aligner was used to map the high-quality reads to Hg19 human genome assembly
Aligned reads were quantified for differential abundance among samples using (a) Partek E/M annotation model and (b) Cufflinks algorithm using the Hg19- Ensemble transcripts release 75
3312 genes were identified
Genome_build: Hg19
Supplementary_files_format_and_content: gene lists reporting Total counts and differential expression analysis

source_name: pancreatic cancer cells

growth_protocol: Cells cultured in RPMI, 10% FBS, 1% penicillin/streptomycin

extract_protocol:
RNA was prepared using Trizol, according to manufacturer's instructions
Libraries were prepared using Illumina’s Truseq Stranded Total-RNA library prep kit and sequenced on a 75 bp pair-end run using the NextSeq 500 High Output Kit 150-cycle; 400 M flow cell)

treatment_protocol: Transduced cells were isolated into single cells by FACS, and colonies were selected with 1 ug/mL puromycin drug pressure

tag: cell line

tag: cell type

value: pancreatic cancer cells

tag: gene insert

value: GRP78 knockdown


title: chipseq_FF_rep2_chip

description: synchronizedtestis_Stra8-FLAGFLAG_FLAGchipseq_2_chip

value: TruSeq ChIP Sample Preparation Kit (Illumina)

value: ChIP protocol:Samples were fixed and sonicated, and the chromatin was immunoprecipitated with the FLAG antibody. For each ChIP sample, a small fraction of the sonicated chromatin was saved as input chromatin control. Crosslinking was reversed, samples were treated with RNaseA and proteinase K, and the DNA was purified.


title: shGRP78 (9)


title: shCTRL (P3)

value: scrambled

value: control


title: 2070.AJ: C24_R3

city: Cologne

first: Deepak

middle: Dharamchand

last: Bhandari

email: bhandari@mpipz.mpg.de, dharamch@msu.edu

state: nrw

address: Carl-von-linne-weg, 10

department: Plant-Microbe interactions

institute: Employer

data_processing:
RNA-seq reads were mapped to the annotated genome of Arabidopsis thaliana (TAIR10) using TOPHAT2 (a = 10, g = 10) and transformed into a read count per gene per sample using the htseq-count script (s = reverse, t = exon) in the package HTSeq
Genes with less than 100 reads in all samples together were discarded; subsequently, the count data of the remaining genes were TMM normalized and log2 transformed using functions ‘calcNormFactors’ and ‘voom’
Differential gene expression over time between the different mutant genotypes and the different treatments, we fitted for each analysis a distinct linear model to the respective log2-transformed count data using the function lmFit  and subsequently performed moderated t-tests for specific comparisons of interest
Genome_build: TAIR10

organism: Arabidopsis thaliana

source_name: 4-week old leaves

growth_protocol: Arabidopsis seeds were grown in soil under controlled environment chambers under a 10 h light regime (150-200 μE/m2s) at 22 °C and 60 % relative humidity

extract_protocol:
Total RNA purification with an RNeasy Plant Mini Kit (Qiagen)
RNA-seq libraries were prepared from 1 µg total RNA according to TruSeq RNA sample preparation v2 guide (Illumina)

treatment_protocol: leaves from four-week-old plants were syringe infiltrated with Pst AvrRps4 or Pst ∆cor AvrRps4 (OD600=0.005). Total RNA isolated from 4 different plants was pooled as a biological replicate. 3 replicates were sequenced.

tag: pathogen

value: Pst ΔCOR AvrRps4

value: 4-week old leaves


title: 2070.AH: C24_R1


title: pep1-1_rep2

value: dissected shoot apices

tag: cultivar

value: Pajares


title: 2070.AI: C24_R2


title: shCTRL (P7)


title: shCTRL (P19)


title: 2070.AE: C24_J1


title: 2070.AG: C24_J3


title: 2070.AB: C24_E1


title: 2070.AF: C24_J2


title: 2070.Y: C24_C1


title: 2070.Z: C24_C2


title: 2070.T: C08_R2


title: 2070.U: C08_R3


title: 2070.AC: C24_E2


title: 2070.AA: C24_C3


title: 2070.AD: C24_E3


title: MM at diagnosis, sample 10

description: MAF translocation

source_name: Highly purified PCs from MM patient

value: Bone marrow plasma cells


title: 2070.S: C08_R1


title: shGRP78 (3)


title: 2070.Q: C08_J2


title: 2070.P: C08_J1


title: 2070.R: C08_J3


title: 2070.O: C08_E3


title: 2070.N: C08_E2


title: Mycorrhized transgenic MtGras1 RNAi roots rep3

description: Gene expression data from mycorrhized transgenic MtGras1 RNAi roots

value: Root

value: Jemalong A17

value: 54 days


title: 2070.L: C08_C3


title: 2070.K: C08_C2


title: 1633.L: R493_24h_r3

value: Pst AvrRps4


title: 2070.J: C08_C1


title: 1633.I: Col_24h_r3


title: 1633.J: eds1_2_24h_r3


title: 1633.K: w.tEDS1_24h_r3


title: 1633.H: R493_8h_r3


title: 1633.E: Col_8h_r3


title: 1633.G: w.tEDS1_8h_r3


title: 1633.D: R493_0h_r3

value: Mock (10mM MgCl2)


title: 1633.C: w.tEDS1_0h_r3


title: 1633.B: eds1_2_0h_r3


title: 1633.A: Col_0h_r3


title: 1633.F: eds1_2_8h_r3


title: 1621.L: R493_24h_r2


title: 1621.J: w.tEDS1_24h_r2


title: 1621.I: Col_24h_r2


title: 1621.G: eds1_2_8h_r2


title: 1621.H: R493_8h_r2


title: 2070.M: C08_E1


title: 1621.K: eds1_2_24h_r2


title: 1621.E: Col_8h_r2


title: 1621.D: R493_0h_r2


title: 1621.F: w.tEDS1_8h_r2


title: 1621.C: eds1_2_0h_r2


title: 1621.B: w.tEDS1_0h_r2


title: 1621.A: Col_0h_r2


title: 1579.L: R493_24h_r1


title: 1579.J: eds1_2_24h_r1


title: 1579.I: Col_24h_r1


title: 1579.H: R493_8h_r1


title: 1579.K: w.tEDS1_24h_r1


title: 1579.F: eds1_2_8h_r1


title: 1579.D: R493_0h_r1


title: 1579.C: w.tEDS1_0h_r1


title: 1579.B: eds1_2_0h_r1


title: 1579.A: Col_0h_r1


title: 1579.G: w.tEDS1_8h_r1


title: mSC_VAT_1231

city: Boston

first: CBDM

last: Lab

address: 77 Avenue Louis Pasteur

department: Microbiology and Immunobiology

institute: Harvard Medical School

data_processing:
fastq quality read filtering (>80% with Sanger Q>20)
cell barcode demultiplexing (custom script)
mapping with tophat2 on the mm10 transcriptome
filtering out low alignment score reads and multiple mapping reads (samtools)
duplication filtering using unique molecular identifiers (custom scripts). UMI sequence is at the 4rth position in the read ID (separation by ":")
Genome_build: mm10
Supplementary_files_format_and_content: raw read counts

source_name: visceral adipose tissue

growth_protocol: Live cells were sorted by flow cytometry from the lymph node, visceral adipose tissue and muscle after cardiotoxin injury (mouse).

extract_protocol:
Lymph nodes were mechanically dissociated. Visceral adipose tissue and muscles were digested with collagenase/dispase to single cell suspensions. mSCs were sorted by flow cytometry as CD45- CD31- Sca-1+ PDGFRα+ PDPN+ cells. Single cells were then encapsulated in nanodroplets according to the InDrops protocol.
mRNA was reverse transcribed using adapters containing oligo dT in each droplet. The emulsion was then broken and linear amplification was performed by in vitro transcription. Illumina adapters were added by reverse transcription and PCR. Library were sequenced using a NextSeq500.

tag: library name

value: visceral adipose tissue

value: male

tag: age (weeks)

tag: total number of reads

tag: number of mapped reads

tag: number of unique reads (umi)


title: mSC_VAT_1225

tag: strain


title: mSC_VAT_1226


title: mSC_VAT_1228


title: mSC_VAT_1230


title: mSC_VAT_1220


title: mSC_VAT_1219


title: mSC_VAT_1227


title: mSC_VAT_1224


title: mSC_VAT_1222


title: mSC_VAT_1229


title: mSC_VAT_1233


title: mSC_VAT_1232


title: mSC_VAT_1234


title: mSC_VAT_1217


title: 1579.E: Col_8h_r1

tag: genotype


title: mSC_VAT_1218

tag: Sex


title: mSC_VAT_1215


title: mSC_VAT_1214


title: mSC_VAT_1216


title: mSC_VAT_1210


title: mSC_VAT_1208


title: mSC_VAT_1209


title: mSC_VAT_1213


title: mSC_VAT_1223


title: mSC_VAT_1212


title: mSC_VAT_1211


title: mSC_VAT_1221


title: mSC_VAT_1196


title: mSC_VAT_1205


title: mSC_VAT_1198


title: mSC_VAT_1201


title: mSC_VAT_1200


title: mSC_VAT_1207


title: mSC_VAT_1197


title: mSC_VAT_1195


title: mSC_VAT_1190


title: mSC_VAT_1193


title: mSC_VAT_1203


title: mSC_VAT_1204


title: mSC_VAT_1199


title: mSC_VAT_1185


title: mSC_VAT_1184


title: mSC_VAT_1189


title: mSC_VAT_1202


title: mSC_VAT_1186


title: mSC_VAT_1192


title: mSC_VAT_1183


title: ATACseq_serum_6day_Pax3_rep1

description:
processed data file:
myog_prog_A_ATAC_peaks.bed
6_day_serum_Pax3_01

value: differentiating mES cells

tag: differentiation protocol

value: serum

tag: time

value: day9 of differentiation

tag: purification

value: PDGFRα+FLK1- sorted

tag: doxycycline-treatment


title: ATACseq_serum_Pax3_rep2

description:
processed data file:
Pax3_EBM_plus_C_ATAC_peaks.bed
serum_Pax3_02

tag: tissue

value: day4 of differentiation


title: mSC_VAT_1182


title: mSC_VAT_1181


title: mSC_VAT_1180


title: mSC_VAT_1206


title: mSC_VAT_1187


title: mSC_VAT_1194


title: mSC_VAT_1191


title: mSC_VAT_1188


title: mSC_VAT_1179


title: mSC_VAT_1177


title: mSC_VAT_1176


title: mSC_VAT_1178


title: mSC_VAT_1172


title: mSC_VAT_1174


title: mSC_VAT_1170


title: mSC_VAT_1171


title: mSC_VAT_1175


title: mSC_VAT_1169


title: mSC_VAT_1164


title: mSC_VAT_1163


title: mSC_VAT_1166


title: mSC_VAT_1168


title: mSC_VAT_1162


title: mSC_VAT_1165


title: mSC_VAT_1167


title: mSC_VAT_1161


title: mSC_VAT_1159


title: mSC_VAT_1160


title: mSC_VAT_1157


title: mSC_VAT_1158


title: mSC_VAT_1155


title: mSC_VAT_1153


title: mSC_VAT_1152


title: mSC_VAT_1151


title: mSC_VAT_1150


title: mSC_VAT_1173


title: mSC_VAT_1146


title: mSC_VAT_1149


title: mSC_VAT_1148


title: mSC_VAT_1147


title: mSC_VAT_1145


title: mSC_VAT_1142


title: mSC_VAT_1141


title: mSC_VAT_1136


title: mSC_VAT_1140


title: mSC_VAT_1143


title: mSC_VAT_1144


title: mSC_VAT_1134


title: mSC_VAT_1156


title: mSC_VAT_1138


title: mSC_VAT_1131


title: mSC_VAT_1132


title: mSC_VAT_1139


title: mSC_VAT_1137


title: mSC_VAT_1130


title: mSC_VAT_1154


title: mSC_VAT_1129


title: mSC_VAT_1128


title: mSC_VAT_1127


title: mSC_VAT_1126


title: mSC_VAT_1124


title: mSC_VAT_1123


title: mSC_VAT_1125


title: mSC_VAT_1122


title: mSC_VAT_1121


title: mSC_VAT_1120


title: mSC_VAT_1118


title: mSC_VAT_1117


title: mSC_VAT_1119


title: mSC_VAT_1133


title: mSC_VAT_1135


title: mSC_VAT_1115


title: mSC_VAT_1114


title: mSC_VAT_1112


title: mSC_VAT_1108


title: mSC_VAT_1106


title: mSC_VAT_1113


title: mSC_VAT_1105


title: mSC_VAT_1104


title: mSC_VAT_1109


title: mSC_VAT_1111


title: mSC_VAT_1103


title: mSC_VAT_1102


title: mSC_VAT_1098


title: mSC_VAT_1099


title: mSC_VAT_1116


title: mSC_VAT_1096


title: mSC_VAT_1095


title: mSC_VAT_1097


title: mSC_VAT_1094


title: mSC_VAT_1093


title: mSC_VAT_1089


title: mSC_VAT_1107


title: mSC_VAT_1087


title: mSC_VAT_1090


title: mSC_VAT_1091


title: mSC_VAT_1110


title: mSC_VAT_1085


title: mSC_VAT_1092


title: mSC_VAT_1088


title: mSC_VAT_1101


title: mSC_VAT_1084


title: mSC_VAT_1086


title: mSC_VAT_1078


title: mSC_VAT_1083


title: mSC_VAT_1100


title: mSC_VAT_1076


title: mSC_VAT_1075


title: mSC_VAT_1082


title: mSC_VAT_1080


title: mSC_VAT_1077


title: mSC_VAT_1074


title: mSC_VAT_1073


title: mSC_VAT_1069


title: mSC_VAT_1070


title: mSC_VAT_1071


title: mSC_VAT_1068


title: mSC_VAT_1067


title: mSC_VAT_1072


title: mSC_VAT_1066


title: mSC_VAT_1081


title: mSC_VAT_1059


title: mSC_VAT_1079


title: mSC_VAT_1063


title: mSC_VAT_1057


title: mSC_VAT_1064


title: mSC_VAT_1060


title: mSC_VAT_1054


title: mSC_VAT_1065


title: mSC_VAT_1061


title: mSC_VAT_1050


title: mSC_VAT_1055


title: mSC_VAT_1058


title: mSC_VAT_1049


title: mSC_VAT_1046


title: mSC_VAT_1053


title: mSC_VAT_1047


title: mSC_VAT_1052


title: mSC_VAT_1056


title: mSC_VAT_1051


title: mSC_VAT_1045


title: mSC_VAT_1043


title: mSC_VAT_1044


title: mSC_VAT_1042


title: mSC_VAT_1041


title: mSC_VAT_1040


title: mSC_VAT_1039


title: mSC_VAT_1038


title: mSC_VAT_1037


title: mSC_VAT_1035


title: mSC_VAT_1034


title: mSC_VAT_1032


title: mSC_VAT_1030


title: mSC_VAT_1031


title: mSC_VAT_1048


title: mSC_VAT_1026


title: mSC_VAT_1027


title: mSC_VAT_1025


title: mSC_VAT_1028


title: mSC_VAT_1022


title: mSC_VAT_1062


title: mSC_VAT_1024


title: mSC_VAT_1019


title: mSC_VAT_1033


title: mSC_VAT_1017


title: mSC_VAT_1029


title: mSC_VAT_1016


title: mSC_VAT_1015


title: mSC_VAT_1014


title: mSC_VAT_1013


title: mSC_VAT_1020


title: mSC_VAT_1011


title: mSC_VAT_1012


title: mSC_VAT_1018


title: mSC_VAT_1036


title: mSC_VAT_1009


title: mSC_VAT_1008


title: mSC_VAT_1006


title: mSC_VAT_1004


title: mSC_VAT_1003


title: mSC_VAT_1005


title: mSC_VAT_1023


title: mSC_VAT_1021


title: mSC_VAT_1001


title: mSC_VAT_999


title: mSC_VAT_997


title: mSC_VAT_996


title: mSC_VAT_1000


title: mSC_VAT_994


title: mSC_VAT_991


title: mSC_VAT_993


title: mSC_VAT_992


title: mSC_VAT_990


title: mSC_VAT_987


title: mSC_VAT_989


title: mSC_VAT_1010


title: mSC_VAT_983


title: mSC_VAT_988


title: mSC_VAT_1007


title: mSC_VAT_986


title: mSC_VAT_984


title: mSC_VAT_985


title: mSC_VAT_982


title: mSC_VAT_1002


title: mSC_VAT_998


title: mSC_VAT_981


title: mSC_VAT_976


title: mSC_VAT_977


title: mSC_VAT_975


title: mSC_VAT_980


title: mSC_VAT_978


title: mSC_VAT_995


title: mSC_VAT_972


title: mSC_VAT_974


title: mSC_VAT_971


title: mSC_VAT_970


title: mSC_VAT_973


title: mSC_VAT_969


title: mSC_VAT_968


title: mSC_VAT_967


title: mSC_VAT_966


title: mSC_VAT_964


title: mSC_VAT_965


title: mSC_VAT_960


title: mSC_VAT_959


title: mSC_VAT_962


title: mSC_VAT_963


title: mSC_VAT_957


title: mSC_VAT_979


title: mSC_VAT_961


title: mSC_VAT_958


title: mSC_VAT_955


title: mSC_VAT_954


title: mSC_VAT_952


title: mSC_VAT_949


title: mSC_VAT_951


title: mSC_VAT_947


title: mSC_VAT_948


title: mSC_VAT_953


title: mSC_VAT_946


title: mSC_VAT_944


title: mSC_VAT_950


title: mSC_VAT_941


title: mSC_VAT_940


title: mSC_VAT_939


title: mSC_VAT_943


title: mSC_VAT_942


title: mSC_VAT_938


title: mSC_VAT_956


title: mSC_VAT_937


title: mSC_VAT_932


title: mSC_VAT_934


title: mSC_VAT_936


title: mSC_VAT_933


title: mSC_VAT_935


title: mSC_VAT_928


title: mSC_VAT_931


title: mSC_VAT_929


title: mSC_VAT_930


title: mSC_VAT_927


title: mSC_VAT_926


title: mSC_VAT_945


title: mSC_VAT_923


title: mSC_VAT_925


title: mSC_VAT_919


title: mSC_VAT_924


title: mSC_VAT_920


title: mSC_VAT_906


title: mSC_VAT_922


title: mSC_VAT_916


title: mSC_VAT_904


title: mSC_VAT_911


title: mSC_VAT_905


title: mSC_VAT_901


title: mSC_VAT_913


title: mSC_VAT_909


title: mSC_VAT_915


title: mSC_VAT_917


title: mSC_VAT_914


title: mSC_VAT_907


title: mSC_VAT_903


title: mSC_VAT_921


title: mSC_VAT_902


title: mSC_VAT_897


title: mSC_VAT_900


title: mSC_VAT_918


title: mSC_VAT_898


title: mSC_VAT_908


title: mSC_VAT_910


title: mSC_VAT_896


title: mSC_VAT_895


title: mSC_VAT_894


title: mSC_VAT_899


title: mSC_VAT_889


title: mSC_VAT_893


title: mSC_VAT_892


title: mSC_VAT_887


title: mSC_VAT_912


title: mSC_VAT_891


title: mSC_VAT_886


title: mSC_VAT_890


title: mSC_VAT_885


title: mSC_VAT_888


title: mSC_VAT_880


title: mSC_VAT_882


title: mSC_VAT_874


title: mSC_VAT_881


title: mSC_VAT_873


title: mSC_VAT_872


title: mSC_VAT_876


title: mSC_VAT_879


title: mSC_VAT_875


title: mSC_VAT_878


title: mSC_VAT_871


title: mSC_VAT_870


title: mSC_VAT_868


title: mSC_VAT_869


title: mSC_VAT_867


title: mSC_VAT_877


title: mSC_VAT_865


title: mSC_VAT_883


title: mSC_VAT_866


title: mSC_VAT_863


title: mSC_VAT_884


title: mSC_VAT_855


title: mSC_VAT_858


title: mSC_VAT_864


title: mSC_VAT_854


title: mSC_VAT_861


title: mSC_VAT_859


title: mSC_VAT_850


title: mSC_VAT_856


title: mSC_VAT_852


title: mSC_VAT_851


title: mSC_VAT_849


title: mSC_VAT_847


title: mSC_VAT_846


title: mSC_VAT_844


title: mSC_VAT_845


title: mSC_VAT_860


title: mSC_VAT_853


title: mSC_VAT_843


title: mSC_VAT_862


title: mSC_VAT_842


title: mSC_VAT_841


title: mSC_VAT_848


title: mSC_VAT_857


title: mSC_VAT_838


title: mSC_VAT_839


title: mSC_VAT_835


title: mSC_VAT_840


title: mSC_VAT_837


title: mSC_VAT_836


title: mSC_VAT_834


title: mSC_VAT_831


title: mSC_VAT_832


title: mSC_VAT_829


title: mSC_VAT_828


title: mSC_VAT_827


title: mSC_VAT_820


title: mSC_VAT_821


title: mSC_VAT_819


title: mSC_VAT_823


title: mSC_VAT_822


title: mSC_VAT_824


title: mSC_VAT_826


title: mSC_VAT_825


title: mSC_VAT_816


title: mSC_VAT_815


title: mSC_VAT_812


title: mSC_VAT_814


title: mSC_VAT_817


title: mSC_VAT_818


title: mSC_VAT_813


title: mSC_VAT_833


title: mSC_VAT_811


title: mSC_VAT_830


title: mSC_VAT_810


title: mSC_VAT_808


title: mSC_VAT_809


title: mSC_VAT_807


title: mSC_VAT_805


title: mSC_VAT_804


title: mSC_VAT_803


title: mSC_VAT_801


title: mSC_VAT_806


title: mSC_VAT_799


title: mSC_VAT_796


title: mSC_VAT_797


title: mSC_VAT_795


title: mSC_VAT_794


title: mSC_VAT_792


title: mSC_VAT_793


title: mSC_VAT_788


title: mSC_VAT_785


title: mSC_VAT_787


title: mSC_VAT_789


title: mSC_VAT_786


title: mSC_VAT_784


title: mSC_VAT_790


title: mSC_VAT_791


title: mSC_VAT_782


title: mSC_VAT_802


title: mSC_VAT_783


title: mSC_VAT_781


title: mSC_VAT_778


title: mSC_VAT_779


title: mSC_VAT_798


title: mSC_VAT_780


title: mSC_VAT_776


title: mSC_VAT_773


title: mSC_VAT_772


title: mSC_VAT_777


title: mSC_VAT_771


title: mSC_VAT_775


title: mSC_VAT_774


title: mSC_VAT_768


title: mSC_VAT_770


title: mSC_VAT_769


title: mSC_VAT_764


title: mSC_VAT_762


title: mSC_VAT_761


title: mSC_VAT_763


title: mSC_VAT_759


title: mSC_VAT_760


title: mSC_VAT_756


title: mSC_VAT_755


title: mSC_VAT_800


title: mSC_VAT_754


title: mSC_VAT_753


title: mSC_VAT_752


title: mSC_VAT_766


title: mSC_VAT_750


title: mSC_VAT_751


title: mSC_VAT_767


title: mSC_VAT_749


title: mSC_VAT_746


title: mSC_VAT_765


title: mSC_VAT_748


title: mSC_VAT_747


title: mSC_VAT_744


title: mSC_VAT_745


title: mSC_VAT_742


title: mSC_VAT_743


title: mSC_VAT_739


title: mSC_VAT_741


title: mSC_VAT_758


title: mSC_VAT_740


title: mSC_VAT_757


title: mSC_VAT_738


title: mSC_VAT_735


title: mSC_VAT_737


title: mSC_VAT_734


title: mSC_VAT_733


title: mSC_VAT_732


title: mSC_VAT_731


title: mSC_VAT_729


title: mSC_VAT_728


title: mSC_VAT_727


title: mSC_VAT_726


title: mSC_VAT_730


title: mSC_VAT_724


title: mSC_VAT_725


title: mSC_VAT_723


title: mSC_VAT_722


title: mSC_VAT_717


title: mSC_VAT_718


title: mSC_VAT_736


title: mSC_VAT_719


title: mSC_VAT_714


title: mSC_VAT_720


title: mSC_VAT_721


title: mSC_VAT_715


title: mSC_VAT_710


title: mSC_VAT_712


title: mSC_VAT_709


title: mSC_VAT_707


title: mSC_VAT_705


title: mSC_VAT_706


title: mSC_VAT_716


title: mSC_VAT_704


title: mSC_VAT_703


title: mSC_VAT_702


title: mSC_VAT_701


title: mSC_VAT_697


title: mSC_VAT_696


title: mSC_VAT_694


title: mSC_VAT_693


title: mSC_VAT_698


title: mSC_VAT_692


title: mSC_VAT_688


title: mSC_VAT_689


title: mSC_VAT_686


title: mSC_VAT_708


title: mSC_VAT_687


title: mSC_VAT_699


title: mSC_VAT_713


title: mSC_VAT_711


title: mSC_VAT_682


title: mSC_VAT_683


title: mSC_VAT_680


title: mSC_VAT_681


title: mSC_VAT_690


title: mSC_VAT_679


title: mSC_VAT_676


title: mSC_VAT_677


title: mSC_VAT_675


title: mSC_VAT_674


title: mSC_VAT_691


title: mSC_VAT_671


title: mSC_VAT_695


title: mSC_VAT_673


title: mSC_VAT_670


title: mSC_VAT_669


title: mSC_VAT_685


title: mSC_VAT_684


title: mSC_VAT_663


title: mSC_VAT_664


title: mSC_VAT_667


title: mSC_VAT_662


title: mSC_VAT_660


title: mSC_VAT_661


title: mSC_VAT_678


title: mSC_VAT_659


title: mSC_VAT_665


title: mSC_VAT_657


title: mSC_VAT_700


title: mSC_VAT_655


title: mSC_VAT_656


title: mSC_VAT_654


title: mSC_VAT_653


title: mSC_VAT_652


title: mSC_VAT_650


title: mSC_VAT_672


title: mSC_VAT_651


title: mSC_VAT_649


title: mSC_VAT_648


title: mSC_VAT_646


title: mSC_VAT_668


title: mSC_VAT_645


title: mSC_VAT_647


title: mSC_VAT_644


title: mSC_VAT_643


title: mSC_VAT_658


title: mSC_VAT_666


title: mSC_VAT_642


title: mSC_VAT_641


title: mSC_VAT_639


title: mSC_VAT_640


title: mSC_VAT_637


title: mSC_VAT_635


title: mSC_VAT_638


title: mSC_VAT_636


title: mSC_VAT_634


title: mSC_VAT_631


title: mSC_VAT_633


title: mSC_VAT_632


title: mSC_VAT_629


title: mSC_VAT_630


title: mSC_VAT_627


title: mSC_VAT_623


title: mSC_VAT_622


title: mSC_VAT_620


title: mSC_VAT_624


title: mSC_VAT_625


title: mSC_VAT_616


title: mSC_VAT_617


title: mSC_VAT_615


title: mSC_VAT_619


title: mSC_VAT_618


title: mSC_VAT_614


title: mSC_VAT_613


title: mSC_VAT_628


title: mSC_VAT_611


title: mSC_VAT_610


title: mSC_VAT_626


title: mSC_VAT_603


title: mSC_VAT_605


title: mSC_VAT_606


title: mSC_VAT_607


title: mSC_VAT_604


title: mSC_VAT_602


title: mSC_VAT_598


title: mSC_VAT_599


title: mSC_VAT_600


title: mSC_VAT_608


title: mSC_VAT_621


title: mSC_VAT_596


title: mSC_VAT_594


title: mSC_VAT_597


title: mSC_VAT_593


title: mSC_VAT_591


title: mSC_VAT_592


title: mSC_VAT_587


title: mSC_VAT_595


title: mSC_VAT_590


title: mSC_VAT_601


title: mSC_VAT_589


title: mSC_VAT_586


title: mSC_VAT_584


title: mSC_VAT_583


title: mSC_VAT_585


title: mSC_VAT_582


title: mSC_VAT_578


title: mSC_VAT_581


title: mSC_VAT_580


title: mSC_VAT_579


title: mSC_VAT_575


title: mSC_VAT_576


title: mSC_VAT_572


title: mSC_VAT_574


title: mSC_VAT_571


title: mSC_VAT_577


title: mSC_VAT_588


title: mSC_VAT_570


title: mSC_VAT_568


title: mSC_VAT_569


title: mSC_VAT_565


title: mSC_VAT_566


title: mSC_VAT_567


title: mSC_VAT_564


title: mSC_VAT_563


title: mSC_VAT_562


title: mSC_VAT_560


title: mSC_VAT_561


title: mSC_VAT_556


title: mSC_VAT_558


title: mSC_VAT_573


title: mSC_VAT_555


title: mSC_VAT_554


title: mSC_VAT_559


title: mSC_VAT_553


title: mSC_VAT_552


title: mSC_VAT_547


title: mSC_VAT_546


title: mSC_VAT_548


title: mSC_VAT_545


title: mSC_VAT_544


title: mSC_VAT_541


title: mSC_VAT_542


title: mSC_VAT_540


title: mSC_VAT_539


title: mSC_VAT_538


title: mSC_VAT_557


title: mSC_VAT_537


title: mSC_VAT_535


title: mSC_VAT_536


title: mSC_VAT_533


title: mSC_VAT_532


title: mSC_VAT_550


title: mSC_VAT_551


title: mSC_VAT_529


title: mSC_VAT_527


title: mSC_VAT_525


title: mSC_VAT_530


title: mSC_VAT_534


title: mSC_VAT_528


title: mSC_VAT_549


title: mSC_VAT_523


title: mSC_VAT_531


title: mSC_VAT_522


title: mSC_VAT_521


title: mSC_VAT_526


title: mSC_VAT_524


title: mSC_VAT_520


title: mSC_VAT_519


title: mSC_VAT_518


title: mSC_VAT_517


title: mSC_VAT_514


title: mSC_VAT_516


title: mSC_VAT_513


title: mSC_VAT_512


title: mSC_VAT_509


title: mSC_VAT_511


title: mSC_VAT_508


title: mSC_VAT_507


title: mSC_VAT_543


title: mSC_VAT_506


title: mSC_VAT_510


title: mSC_VAT_504


title: mSC_VAT_505


title: mSC_VAT_501


title: mSC_VAT_503


title: mSC_VAT_502


title: mSC_VAT_500


title: mSC_VAT_499


title: mSC_VAT_498


title: mSC_VAT_497


title: mSC_VAT_496


title: mSC_VAT_495


title: mSC_VAT_612


title: mSC_VAT_494


title: mSC_VAT_493


title: mSC_VAT_515


title: mSC_VAT_492


title: mSC_VAT_490


title: mSC_VAT_609


title: mSC_VAT_489


title: mSC_VAT_487


title: mSC_VAT_488


title: mSC_VAT_484


title: mSC_VAT_486


title: mSC_VAT_485


title: mSC_VAT_483


title: mSC_VAT_481


title: mSC_VAT_479


title: mSC_VAT_482


title: mSC_VAT_480


title: mSC_VAT_476


title: mSC_VAT_474


title: mSC_VAT_477


title: mSC_VAT_478


title: mSC_VAT_471


title: mSC_VAT_472


title: mSC_VAT_475


title: mSC_VAT_470


title: mSC_VAT_473


title: mSC_VAT_491


title: mSC_VAT_467


title: mSC_VAT_469


title: mSC_VAT_466


title: mSC_VAT_465


title: mSC_VAT_464


title: mSC_VAT_463


title: mSC_VAT_461


title: mSC_VAT_459


title: mSC_VAT_460


title: mSC_VAT_456


title: mSC_VAT_455


title: mSC_VAT_454


title: mSC_VAT_450


title: mSC_VAT_453


title: mSC_VAT_451


title: mSC_VAT_452


title: mSC_VAT_468


title: mSC_VAT_449


title: mSC_VAT_448


title: mSC_VAT_439


title: mSC_VAT_441


title: mSC_VAT_442


title: mSC_VAT_440


title: mSC_VAT_447


title: mSC_VAT_446


title: mSC_VAT_436


title: mSC_VAT_437


title: mSC_VAT_443


title: mSC_VAT_434


title: mSC_VAT_458


title: mSC_VAT_435


title: mSC_VAT_462


title: mSC_VAT_457


title: mSC_VAT_433


title: mSC_VAT_432


title: mSC_VAT_431


title: mSC_VAT_430


title: mSC_VAT_427


title: mSC_VAT_426


title: mSC_VAT_425


title: mSC_VAT_428


title: mSC_VAT_424


title: mSC_VAT_445


title: mSC_VAT_423


title: mSC_VAT_429


title: mSC_VAT_438


title: mSC_VAT_422


title: mSC_VAT_421


title: mSC_VAT_444


title: mSC_VAT_420


title: mSC_VAT_415


title: mSC_VAT_414


title: mSC_VAT_417


title: mSC_VAT_419


title: mSC_VAT_410


title: mSC_VAT_409


title: mSC_VAT_411


title: mSC_VAT_412


title: mSC_VAT_416


title: mSC_VAT_407


title: mSC_VAT_406


title: mSC_VAT_405


title: mSC_VAT_404


title: mSC_VAT_403


title: mSC_VAT_402


title: mSC_VAT_400


title: mSC_VAT_398


title: mSC_VAT_399


title: mSC_VAT_396


title: mSC_VAT_397


title: mSC_VAT_408


title: mSC_VAT_395


title: mSC_VAT_394


title: mSC_VAT_418


title: mSC_VAT_392


title: mSC_VAT_393


title: mSC_VAT_390


title: mSC_VAT_391


title: mSC_VAT_388


title: mSC_VAT_387


title: mSC_VAT_401


title: mSC_VAT_380


title: mSC_VAT_382


title: mSC_VAT_383


title: mSC_VAT_385


title: mSC_VAT_384


title: mSC_VAT_381


title: mSC_VAT_379


title: mSC_VAT_377


title: mSC_VAT_378


title: mSC_VAT_375


title: mSC_VAT_373


title: mSC_VAT_372


title: mSC_VAT_374


title: mSC_VAT_367


title: mSC_VAT_368


title: mSC_VAT_369


title: mSC_VAT_371


title: mSC_VAT_370


title: mSC_VAT_366


title: mSC_VAT_363


title: mSC_VAT_389


title: mSC_VAT_362


title: mSC_VAT_361


title: mSC_VAT_360


title: mSC_VAT_365


title: mSC_VAT_359


title: mSC_VAT_413


title: mSC_VAT_358


title: mSC_VAT_354


title: mSC_VAT_352


title: mSC_VAT_353


title: mSC_VAT_376


title: mSC_VAT_355


title: mSC_VAT_351


title: mSC_VAT_357


title: mSC_VAT_386


title: mSC_VAT_347


title: mSC_VAT_349


title: mSC_VAT_346


title: mSC_VAT_364


title: mSC_VAT_345


title: mSC_VAT_342


title: mSC_VAT_343


title: mSC_VAT_341


title: mSC_VAT_340


title: mSC_VAT_344


title: mSC_VAT_348


title: mSC_VAT_339


title: mSC_VAT_338


title: mSC_VAT_337


title: mSC_VAT_336


title: mSC_VAT_356


title: mSC_VAT_335


title: mSC_VAT_331


title: mSC_VAT_332


title: mSC_VAT_327


title: mSC_VAT_325


title: mSC_VAT_324


title: mSC_VAT_330


title: mSC_VAT_322


title: mSC_VAT_326


title: mSC_VAT_329


title: mSC_VAT_323


title: mSC_VAT_334


title: mSC_VAT_333


title: mSC_VAT_328


title: mSC_VAT_314


title: mSC_VAT_320


title: mSC_VAT_315


title: mSC_VAT_316


title: mSC_VAT_318


title: mSC_VAT_317


title: mSC_VAT_306


title: mSC_VAT_310


title: mSC_VAT_308


title: mSC_VAT_319


title: mSC_VAT_309


title: mSC_VAT_305


title: mSC_VAT_304


title: mSC_VAT_311


title: mSC_VAT_321


title: mSC_VAT_307


title: mSC_VAT_312


title: mSC_VAT_302


title: mSC_VAT_303


title: mSC_VAT_301


title: mSC_VAT_299


title: mSC_VAT_300


title: mSC_VAT_297


title: mSC_VAT_298


title: mSC_VAT_313


title: mSC_VAT_296


title: mSC_VAT_295


title: mSC_VAT_294


title: mSC_VAT_288


title: mSC_VAT_290


title: mSC_VAT_286


title: mSC_VAT_292


title: mSC_VAT_283


title: mSC_VAT_291


title: mSC_VAT_287


title: mSC_VAT_293


title: mSC_VAT_279


title: mSC_VAT_277


title: mSC_VAT_285


title: mSC_VAT_282


title: mSC_VAT_278


title: mSC_VAT_289


title: mSC_VAT_281


title: mSC_VAT_273


title: mSC_VAT_272


title: mSC_VAT_275


title: mSC_VAT_271


title: mSC_VAT_274


title: mSC_VAT_280


title: mSC_VAT_269


title: mSC_VAT_268


title: mSC_VAT_267


title: mSC_VAT_266


title: mSC_VAT_265


title: mSC_VAT_284


title: mSC_VAT_264


title: mSC_VAT_263


title: mSC_VAT_276


title: mSC_VAT_262


title: mSC_VAT_261


title: mSC_VAT_258


title: mSC_VAT_259


title: mSC_VAT_257


title: mSC_VAT_260


title: mSC_VAT_254


title: mSC_VAT_255


title: mSC_VAT_252


title: mSC_VAT_250


title: mSC_VAT_270


title: mSC_VAT_253


title: mSC_VAT_350


title: mSC_VAT_244


title: mSC_VAT_242


title: mSC_VAT_246


title: mSC_VAT_243


title: mSC_VAT_251


title: mSC_VAT_245


title: mSC_VAT_241


title: mSC_VAT_240


title: mSC_VAT_239


title: mSC_VAT_238


title: mSC_VAT_234


title: mSC_VAT_237


title: mSC_VAT_256


title: mSC_VAT_233


title: mSC_VAT_235


title: mSC_VAT_232


title: mSC_VAT_236


title: mSC_VAT_247


title: mSC_VAT_248


title: mSC_VAT_226


title: mSC_VAT_249


title: mSC_VAT_229


title: mSC_VAT_225


title: mSC_VAT_228


title: mSC_VAT_230


title: mSC_VAT_223


title: mSC_VAT_217


title: mSC_VAT_219


title: mSC_VAT_224


title: mSC_VAT_221


title: mSC_VAT_227


title: mSC_VAT_220


title: mSC_VAT_215


title: mSC_VAT_216


title: mSC_VAT_214


title: mSC_VAT_218


title: mSC_VAT_213


title: mSC_VAT_222


title: mSC_VAT_231


title: mSC_VAT_212


title: mSC_VAT_211


title: mSC_VAT_210


title: mSC_VAT_207


title: mSC_VAT_206


title: mSC_VAT_200


title: mSC_VAT_203


title: mSC_VAT_199


title: mSC_VAT_201


title: mSC_VAT_208


title: mSC_VAT_205


title: mSC_VAT_197


title: mSC_VAT_202


title: mSC_VAT_196


title: mSC_VAT_209


title: mSC_VAT_204


title: mSC_VAT_198


title: mSC_VAT_195


title: mSC_VAT_193


title: mSC_VAT_190


title: mSC_VAT_191


title: mSC_VAT_192


title: mSC_VAT_188


title: mSC_VAT_187


title: mSC_VAT_186


title: mSC_VAT_183


title: mSC_VAT_185


title: mSC_VAT_182


title: mSC_VAT_180


title: mSC_VAT_184


title: mSC_VAT_179


title: mSC_VAT_178


title: mSC_VAT_194


title: mSC_VAT_177


title: mSC_VAT_176


title: mSC_VAT_189


title: mSC_VAT_175


title: mSC_VAT_169


title: mSC_VAT_172


title: mSC_VAT_171


title: mSC_VAT_174


title: mSC_VAT_168


title: mSC_VAT_173


title: mSC_VAT_166


title: mSC_VAT_167


title: mSC_VAT_165


title: mSC_VAT_170


title: mSC_VAT_163


title: mSC_VAT_164


title: mSC_VAT_162


title: mSC_VAT_159


title: mSC_VAT_161


title: mSC_VAT_158


title: mSC_VAT_181


title: mSC_VAT_157


title: mSC_VAT_156


title: mSC_VAT_155


title: mSC_VAT_154


title: mSC_VAT_152


title: mSC_VAT_153


title: mSC_VAT_150


title: mSC_VAT_147


title: mSC_VAT_149


title: mSC_VAT_145


title: mSC_VAT_148


title: mSC_VAT_142


title: mSC_VAT_144


title: mSC_VAT_141


title: mSC_VAT_160


title: mSC_VAT_140


title: mSC_VAT_137


title: mSC_VAT_139


title: mSC_VAT_138


title: mSC_VAT_143


title: mSC_VAT_135


title: mSC_VAT_136


title: mSC_VAT_133


title: mSC_VAT_132


title: mSC_VAT_131


title: mSC_VAT_134


title: mSC_VAT_128


title: mSC_VAT_127


title: mSC_VAT_126


title: mSC_VAT_129


title: mSC_VAT_146


title: mSC_VAT_124


title: mSC_VAT_123


title: mSC_VAT_120


title: mSC_VAT_118


title: mSC_VAT_121


title: mSC_VAT_151


title: mSC_VAT_114


title: mSC_VAT_115


title: mSC_VAT_111


title: mSC_VAT_110


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title: mSC_VAT_109


title: mSC_VAT_108


title: mSC_VAT_113


title: mSC_VAT_130


title: mSC_VAT_107


title: mSC_VAT_106


title: mSC_VAT_117


title: mSC_VAT_105


title: mSC_VAT_104


title: mSC_VAT_125


title: mSC_VAT_102


title: mSC_VAT_101


title: mSC_VAT_103


title: mSC_VAT_100


title: mSC_VAT_99


title: mSC_VAT_119


title: mSC_VAT_98


title: mSC_VAT_116


title: mSC_VAT_112


title: mSC_VAT_92


title: mSC_VAT_96


title: mSC_VAT_93


title: mSC_VAT_94


title: mSC_VAT_97


title: mSC_VAT_91


title: mSC_VAT_95


title: mSC_VAT_90


title: mSC_VAT_87


title: mSC_VAT_86


title: mSC_VAT_85


title: mSC_VAT_89


title: mSC_VAT_88


title: mSC_VAT_82


title: mSC_VAT_80


title: mSC_VAT_79


title: mSC_VAT_83


title: mSC_VAT_78


title: mSC_VAT_81


title: mSC_VAT_75


title: mSC_VAT_76


title: mSC_VAT_74


title: mSC_VAT_77


title: mSC_VAT_72


title: mSC_VAT_71


title: mSC_VAT_73


title: mSC_VAT_70


title: mSC_VAT_69


title: mSC_VAT_67


title: mSC_VAT_68


title: mSC_VAT_84


title: mSC_VAT_66


title: mSC_VAT_59


title: mSC_VAT_65


title: mSC_VAT_60


title: mSC_VAT_63


title: mSC_VAT_61


title: mSC_VAT_62


title: mSC_VAT_57


title: mSC_VAT_64


title: mSC_VAT_58


title: mSC_VAT_53


title: mSC_VAT_54


title: mSC_VAT_55


title: mSC_VAT_52


title: mSC_VAT_51


title: mSC_VAT_49


title: mSC_VAT_48


title: mSC_VAT_47


title: mSC_VAT_46


title: mSC_VAT_45


title: mSC_VAT_44


title: mSC_VAT_43


title: mSC_VAT_42


title: mSC_VAT_40


title: mSC_VAT_41


title: mSC_VAT_38


title: mSC_VAT_37


title: mSC_VAT_39


title: mSC_VAT_56


title: mSC_VAT_35


title: mSC_VAT_34


title: mSC_VAT_36


title: mSC_VAT_29


title: mSC_VAT_33


title: mSC_VAT_32


title: mSC_VAT_28


title: mSC_VAT_50


title: mSC_VAT_26


title: mSC_VAT_27


title: mSC_VAT_24


title: mSC_VAT_25


title: mSC_VAT_22


title: mSC_VAT_18


title: mSC_VAT_17


title: mSC_VAT_16


title: mSC_VAT_15


title: mSC_VAT_14


title: mSC_VAT_13


title: mSC_VAT_12


title: mSC_VAT_10


title: mSC_VAT_11


title: mSC_VAT_9


title: mSC_VAT_8


title: mSC_VAT_31


title: mSC_VAT_30


title: mSC_VAT_23


title: mSC_VAT_7


title: mSC_VAT_6


title: mSC_VAT_5


title: mSC_VAT_3


title: mSC_VAT_2


title: mSC_VAT_21


title: mSC_VAT_1


title: mSC_LN_1683

source_name: lymph node

value: lymph node


title: mSC_VAT_20


title: mSC_LN_1685


title: mSC_LN_1686


title: mSC_LN_1681


title: mSC_LN_1680


title: mSC_LN_1678


title: mSC_LN_1679


title: mSC_LN_1677


title: mSC_LN_1676


title: mSC_LN_1674


title: mSC_LN_1672


title: mSC_LN_1673


title: mSC_LN_1675


title: mSC_LN_1669


title: mSC_LN_1671


title: mSC_LN_1670


title: mSC_LN_1668


title: mSC_VAT_4


title: mSC_LN_1684


title: mSC_LN_1667


title: mSC_LN_1666


title: mSC_LN_1665


title: mSC_LN_1664


title: mSC_LN_1661


title: mSC_LN_1662


title: mSC_LN_1682


title: mSC_LN_1660


title: mSC_LN_1663


title: mSC_LN_1656


title: mSC_LN_1659


title: mSC_LN_1654


title: mSC_LN_1657


title: mSC_LN_1655


title: mSC_VAT_19


title: mSC_LN_1652


title: mSC_LN_1658


title: mSC_LN_1653


title: mSC_LN_1651


title: mSC_LN_1649


title: mSC_LN_1648


title: mSC_LN_1644


title: mSC_LN_1646


title: mSC_LN_1647


title: mSC_LN_1645


title: mSC_LN_1643


title: mSC_LN_1642


title: mSC_LN_1639


title: mSC_LN_1640


title: mSC_LN_1641


title: mSC_LN_1633


title: mSC_LN_1637


title: mSC_LN_1631


title: mSC_LN_1638


title: mSC_LN_1635


title: mSC_LN_1636


title: mSC_LN_1632


title: mSC_LN_1650


title: mSC_LN_1630


title: mSC_LN_1629


title: mSC_LN_1626


title: mSC_LN_1627


title: mSC_LN_1625


title: mSC_LN_1622


title: mSC_LN_1623


title: mSC_LN_1624


title: mSC_LN_1620


title: mSC_LN_1618


title: mSC_LN_1619


title: mSC_LN_1621


title: mSC_LN_1634


title: mSC_LN_1614


title: mSC_LN_1613


title: mSC_LN_1612


title: mSC_LN_1610


title: mSC_LN_1609


title: mSC_LN_1616


title: mSC_LN_1617


title: mSC_LN_1611


title: mSC_LN_1606


title: mSC_LN_1608


title: mSC_LN_1605


title: mSC_LN_1602


title: mSC_LN_1615


title: mSC_LN_1628


title: mSC_LN_1601


title: mSC_LN_1599


title: mSC_LN_1597


title: mSC_LN_1596


title: mSC_LN_1595


title: mSC_LN_1594


title: mSC_LN_1592


title: mSC_LN_1591


title: mSC_LN_1590


title: mSC_LN_1593


title: mSC_LN_1589


title: mSC_LN_1607


title: mSC_LN_1604


title: mSC_LN_1603


title: mSC_LN_1582


title: mSC_LN_1584


title: mSC_LN_1588


title: mSC_LN_1580


title: mSC_LN_1581


title: mSC_LN_1586


title: mSC_LN_1587


title: mSC_LN_1583


title: mSC_LN_1578


title: mSC_LN_1598


title: mSC_LN_1575


title: mSC_LN_1579


title: mSC_LN_1577


title: mSC_LN_1576


title: mSC_LN_1600


title: mSC_LN_1574


title: mSC_LN_1573


title: mSC_LN_1571


title: mSC_LN_1570


title: mSC_LN_1585


title: mSC_LN_1569


title: mSC_LN_1566


title: mSC_LN_1567


title: mSC_LN_1564


title: mSC_LN_1572


title: mSC_LN_1568


title: mSC_LN_1565


title: mSC_LN_1563


title: mSC_LN_1562


title: mSC_LN_1559


title: mSC_LN_1560


title: mSC_LN_1558


title: mSC_LN_1561


title: mSC_Muscle_1611

source_name: muscle

value: muscle


title: mSC_Muscle_1613


title: mSC_Muscle_1618


title: mSC_Muscle_1610


title: mSC_Muscle_1619


title: mSC_Muscle_1617


title: mSC_Muscle_1609


title: mSC_Muscle_1607


title: mSC_Muscle_1608


title: mSC_Muscle_1615


title: mSC_Muscle_1614


title: mSC_Muscle_1616


title: mSC_Muscle_1605


title: mSC_Muscle_1603


title: mSC_Muscle_1604


title: mSC_Muscle_1600


title: mSC_Muscle_1601


title: mSC_Muscle_1602


title: mSC_Muscle_1598


title: mSC_LN_1557


title: mSC_Muscle_1597


title: mSC_Muscle_1596


title: mSC_Muscle_1595


title: mSC_Muscle_1594


title: mSC_Muscle_1612


title: mSC_Muscle_1593


title: mSC_Muscle_1591


title: mSC_Muscle_1606


title: mSC_Muscle_1592


title: mSC_Muscle_1589


title: mSC_Muscle_1581


title: mSC_Muscle_1587


title: mSC_Muscle_1588


title: mSC_Muscle_1586


title: mSC_Muscle_1583


title: mSC_Muscle_1585


title: mSC_Muscle_1578


title: mSC_Muscle_1579


title: mSC_Muscle_1582


title: mSC_Muscle_1576


title: mSC_Muscle_1580


title: mSC_Muscle_1599


title: mSC_Muscle_1584


title: mSC_Muscle_1590


title: mSC_Muscle_1577


title: mSC_Muscle_1574


title: mSC_Muscle_1575


title: mSC_Muscle_1569


title: mSC_Muscle_1568


title: mSC_Muscle_1573


title: mSC_Muscle_1571


title: mSC_Muscle_1566


title: mSC_Muscle_1565


title: mSC_Muscle_1567


title: mSC_Muscle_1564


title: mSC_Muscle_1563


title: mSC_Muscle_1562


title: mSC_Muscle_1561


title: mSC_Muscle_1559


title: mSC_Muscle_1558


title: mSC_Muscle_1560


title: mSC_Muscle_1572


title: mSC_Muscle_1554


title: mSC_Muscle_1552


title: mSC_Muscle_1557


title: mSC_Muscle_1553


title: mSC_Muscle_1555


title: mSC_Muscle_1547


title: mSC_Muscle_1550


title: mSC_Muscle_1548


title: mSC_Muscle_1551


title: mSC_Muscle_1549


title: mSC_Muscle_1544


title: mSC_Muscle_1570


title: mSC_Muscle_1537


title: mSC_Muscle_1542


title: mSC_Muscle_1535


title: mSC_Muscle_1540


title: mSC_Muscle_1539


title: mSC_Muscle_1536


title: mSC_Muscle_1534


title: mSC_Muscle_1543


title: mSC_Muscle_1556


title: mSC_Muscle_1533


title: mSC_Muscle_1532


title: mSC_Muscle_1531


title: mSC_Muscle_1529


title: mSC_Muscle_1545


title: mSC_Muscle_1528


title: mSC_Muscle_1546


title: mSC_Muscle_1527


title: mSC_Muscle_1525


title: mSC_Muscle_1538


title: mSC_Muscle_1520


title: mSC_Muscle_1521


title: mSC_Muscle_1519


title: mSC_Muscle_1518


title: mSC_Muscle_1541


title: mSC_Muscle_1530


title: mSC_Muscle_1517


title: mSC_Muscle_1522


title: mSC_Muscle_1526


title: mSC_Muscle_1514


title: mSC_Muscle_1511


title: mSC_Muscle_1512


title: mSC_Muscle_1509


title: mSC_Muscle_1513


title: mSC_Muscle_1515


title: mSC_Muscle_1510


title: mSC_Muscle_1507


title: mSC_Muscle_1508


title: mSC_Muscle_1506


title: mSC_Muscle_1505


title: mSC_Muscle_1504


title: mSC_Muscle_1503


title: mSC_Muscle_1516


title: mSC_Muscle_1500


title: mSC_Muscle_1501


title: mSC_Muscle_1523


title: mSC_Muscle_1493


title: mSC_Muscle_1502


title: mSC_Muscle_1494


title: mSC_Muscle_1492


title: mSC_Muscle_1498


title: mSC_Muscle_1495


title: mSC_Muscle_1497


title: mSC_Muscle_1491


title: mSC_Muscle_1490


title: mSC_Muscle_1496


title: mSC_Muscle_1489


title: mSC_Muscle_1486


title: mSC_Muscle_1488


title: mSC_Muscle_1487


title: mSC_Muscle_1485


title: mSC_Muscle_1484


title: mSC_Muscle_1483


title: mSC_Muscle_1480


title: mSC_Muscle_1481


title: mSC_Muscle_1499


title: mSC_Muscle_1478


title: mSC_Muscle_1479


title: mSC_Muscle_1476


title: mSC_Muscle_1477


title: mSC_Muscle_1475


title: mSC_Muscle_1473


title: mSC_Muscle_1471


title: mSC_Muscle_1465


title: mSC_Muscle_1469


title: mSC_Muscle_1467


title: mSC_Muscle_1466


title: mSC_Muscle_1524


title: mSC_Muscle_1464


title: mSC_Muscle_1468


title: mSC_Muscle_1459


title: mSC_Muscle_1458


title: mSC_Muscle_1460


title: mSC_Muscle_1482


title: mSC_Muscle_1461


title: mSC_Muscle_1470


title: mSC_Muscle_1457


title: mSC_Muscle_1472


title: mSC_Muscle_1462


title: mSC_Muscle_1456


title: mSC_Muscle_1455


title: mSC_Muscle_1463


title: mSC_Muscle_1453


title: mSC_Muscle_1454


title: mSC_Muscle_1452


title: mSC_Muscle_1474


title: mSC_Muscle_1449


title: mSC_Muscle_1448


title: mSC_Muscle_1446


title: mSC_Muscle_1447


title: mSC_Muscle_1445


title: mSC_Muscle_1444


title: mSC_Muscle_1442


title: mSC_Muscle_1441


title: mSC_Muscle_1439


title: mSC_Muscle_1443


title: mSC_Muscle_1436


title: mSC_Muscle_1434


title: mSC_Muscle_1435


title: mSC_Muscle_1437


title: mSC_Muscle_1433


title: mSC_Muscle_1440


title: mSC_Muscle_1431


title: mSC_Muscle_1451


title: mSC_Muscle_1450


title: mSC_Muscle_1424


title: mSC_Muscle_1426


title: mSC_Muscle_1428


title: mSC_Muscle_1423


title: mSC_Muscle_1430


title: mSC_Muscle_1422


title: mSC_Muscle_1429


title: mSC_Muscle_1427


title: mSC_Muscle_1425


title: mSC_Muscle_1421


title: mSC_Muscle_1419


title: mSC_Muscle_1420


title: mSC_Muscle_1417


title: mSC_Muscle_1432


title: mSC_Muscle_1438


title: mSC_Muscle_1413


title: mSC_Muscle_1411


title: mSC_Muscle_1412


title: mSC_Muscle_1415


title: mSC_Muscle_1409


title: mSC_Muscle_1410


title: mSC_Muscle_1406


title: mSC_Muscle_1405


title: mSC_Muscle_1402


title: mSC_Muscle_1404


title: mSC_Muscle_1418


title: mSC_Muscle_1396


title: mSC_Muscle_1400


title: mSC_Muscle_1397


title: mSC_Muscle_1401


title: mSC_Muscle_1394


title: mSC_Muscle_1399


title: mSC_Muscle_1393


title: mSC_Muscle_1392


title: mSC_Muscle_1416


title: mSC_Muscle_1390


title: mSC_Muscle_1391


title: mSC_Muscle_1403


title: mSC_Muscle_1407


title: mSC_Muscle_1395


title: mSC_Muscle_1388


title: mSC_Muscle_1414


title: mSC_Muscle_1389


title: mSC_Muscle_1398


title: mSC_Muscle_1384


title: mSC_Muscle_1387


title: mSC_Muscle_1408


title: mSC_Muscle_1386


title: mSC_Muscle_1383


title: mSC_Muscle_1382


title: mSC_Muscle_1379


title: mSC_Muscle_1380


title: mSC_Muscle_1378


title: mSC_Muscle_1375


title: mSC_Muscle_1376


title: mSC_Muscle_1377


title: mSC_Muscle_1370


title: mSC_Muscle_1373


title: mSC_Muscle_1371


title: mSC_Muscle_1369


title: mSC_Muscle_1374


title: mSC_Muscle_1366


title: mSC_Muscle_1367


title: mSC_Muscle_1365


title: mSC_Muscle_1361


title: mSC_Muscle_1359


title: mSC_Muscle_1363


title: mSC_Muscle_1357


title: mSC_Muscle_1360


title: mSC_Muscle_1385


title: mSC_Muscle_1381


title: mSC_Muscle_1354


title: mSC_Muscle_1364


title: mSC_Muscle_1358


title: mSC_Muscle_1356


title: mSC_Muscle_1352


title: mSC_Muscle_1350


title: mSC_Muscle_1372


title: mSC_Muscle_1348


title: mSC_Muscle_1347


title: mSC_Muscle_1346


title: mSC_Muscle_1362


title: mSC_Muscle_1349


title: mSC_Muscle_1344


title: mSC_Muscle_1343


title: mSC_Muscle_1353


title: mSC_Muscle_1345


title: mSC_Muscle_1341


title: mSC_Muscle_1342


title: mSC_Muscle_1340


title: mSC_Muscle_1339


title: mSC_Muscle_1355


title: mSC_Muscle_1338


title: mSC_Muscle_1335


title: mSC_Muscle_1331


title: mSC_Muscle_1333


title: mSC_Muscle_1334


title: mSC_Muscle_1332


title: mSC_Muscle_1330


title: mSC_Muscle_1351


title: mSC_Muscle_1329


title: mSC_Muscle_1328


title: mSC_Muscle_1368


title: mSC_Muscle_1327


title: mSC_Muscle_1326


title: mSC_Muscle_1325


title: mSC_Muscle_1323


title: mSC_Muscle_1320


title: mSC_Muscle_1321


title: mSC_Muscle_1322


title: mSC_Muscle_1319


title: mSC_Muscle_1317


title: mSC_Muscle_1318


title: mSC_Muscle_1315


title: mSC_Muscle_1314


title: mSC_Muscle_1313


title: mSC_Muscle_1337


title: mSC_Muscle_1336


title: mSC_Muscle_1312


title: mSC_Muscle_1311


title: mSC_Muscle_1310


title: mSC_Muscle_1309


title: mSC_Muscle_1306


title: mSC_Muscle_1305


title: mSC_Muscle_1307


title: mSC_Muscle_1303


title: mSC_Muscle_1304


title: mSC_Muscle_1301


title: mSC_Muscle_1299


title: mSC_Muscle_1324


title: mSC_Muscle_1302


title: mSC_Muscle_1300


title: mSC_Muscle_1298


title: mSC_Muscle_1297


title: mSC_Muscle_1316


title: mSC_Muscle_1293


title: mSC_Muscle_1294


title: mSC_Muscle_1296


title: mSC_Muscle_1290


title: mSC_Muscle_1288


title: mSC_Muscle_1289


title: mSC_Muscle_1291


title: mSC_Muscle_1292


title: mSC_Muscle_1287


title: mSC_Muscle_1308


title: mSC_Muscle_1283


title: mSC_Muscle_1284


title: mSC_Muscle_1282


title: mSC_Muscle_1286


title: mSC_Muscle_1279


title: mSC_Muscle_1277


title: mSC_Muscle_1276


title: mSC_Muscle_1281


title: mSC_Muscle_1285


title: mSC_Muscle_1280


title: mSC_Muscle_1278


title: mSC_Muscle_1274


title: mSC_Muscle_1270


title: mSC_Muscle_1272


title: mSC_Muscle_1295


title: mSC_Muscle_1269


title: mSC_Muscle_1271


title: mSC_Muscle_1268


title: mSC_Muscle_1273


title: mSC_Muscle_1267


title: mSC_Muscle_1265


title: mSC_Muscle_1266


title: mSC_Muscle_1264


title: mSC_Muscle_1263


title: mSC_Muscle_1260


title: mSC_Muscle_1261


title: mSC_Muscle_1258


title: mSC_Muscle_1259


title: mSC_Muscle_1257


title: mSC_Muscle_1275


title: mSC_Muscle_1252


title: mSC_Muscle_1253


title: mSC_Muscle_1256


title: mSC_Muscle_1251