title: Post-transplant sample of DBD patient 11

description:
Gene expression data from DBD donor lungs 2 hours after reperfusion, this is post-transplant data of sample No_45
sample name: No_55


title: Post-transplant sample of DBD patient 09

status: Public on Nov 12 2009

type: RNA

city: Seoul

first: Chang-Hyun

last: Kang

address: Yeongun-dong, Chongro-gu

country: South Korea

institute: Seoul National University Hospital

description:
Gene expression data from DBD donor lungs 2 hours after reperfusion, this is post-transplant data of sample No_43
sample name: No_53

hyb_protocol: Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG U-133 plus2. GeneChips were washed and stained in the Affymetrix Fluidics Station 4500.

scan_protocol: GeneChips were scanned using the GeneChip scanner 3700 7G

data_processing: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.

label: biotin

molecule: total RNA

organism: Homo sapiens

source_name: DBD post-transplant sample

label_protocol: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).

extract_protocol: Trizol extraction of total RNA was performed according to the manufacturer's instructions.

tag: tissue

value: lung

tag: donation source

value: DBD

tag: sampling time

value: post-transplant


title: Post-transplant sample of DBD patient 05

description:
Gene expression data from DBD donor lungs 2 hours after reperfusion, this is post-transplant data of sample No_25
sample name: No_35


title: Pre-transplant sample of DBD patient 09

description:
Gene expression data from DBD donor lungs just after preservation
sample name: No_43

source_name: DBD pre-transplant sample

value: pre-transplant


title: Post-transplant sample of DBD patient 06

description:
Gene expression data from DBD donor lungs 2 hours after reperfusion, this is post-transplant data of sample No_26
sample name: No_36


title: Pre-transplant sample of DBD patient 08

description:
Gene expression data from DBD donor lungs just after preservation
sample name: No_42


title: Post-transplant sample of DBD patient 10

description:
Gene expression data from DBD donor lungs 2 hours after reperfusion, this is post-transplant data of sample No_44
sample name: No_54


title: Pre-transplant sample of DBD patient 12

description:
Gene expression data from DBD donor lungs just after preservation
sample name: No_46


title: Pre-transplant sample of DBD patient 05

description:
Gene expression data from DBD donor lungs just after preservation
sample name: No_25


title: Post-transplant sample of DBD patient 03

description:
Gene expression data from DBD donor lungs 2 hours after reperfusion, this is post-transplant data of sample No_23
sample name: No_33


title: Post-transplant sample of DBD patient 04

description:
Gene expression data from DBD donor lungs 2 hours after reperfusion, this is post-transplant data of sample No_24
sample name: No_34


title: Pre-transplant sample of DBD patient 04

description:
Gene expression data from DBD donor lungs just after preservation
sample name: No_24


title: Cardiac response to stable HIF-1alpha overexpression for three days rep 2

status: Public on Dec 02 2008

city: Honolulu

first: Chad

middle: Brian

last: Walton

state: HI

address: 651 Ilalo Street, JABSOM BSB 222-18

department: Center for Cardiovascular Research

country: USA

institute: University of Hawaii

description: This is a standard 2 color hybridization to determine transcript-level dysregulation in the heart in response to the overexpression of a stable transcription fcator.

hyb_protocol: Labeled probe plus Cot1, polyA, tRNA combined with 3X SSC plus 25% formamide. 16 hour hybridization with glass coverslips at 42 degrees C. Stringency washing according to manufacturer's protocol.

scan_protocol: Standard scan methodology with Genepix 4000B device(Molecular Devices)

data_processing: Results were normalized using the ratio of medians method and filtered to indicate a Genepix flag.

organism: Mus musculus

source_name: Whole heart tissue from hearts induced to express a mutated, stable form of HIF-1alpha

growth_protocol: In vivo cardiac-specific expression of a mutated protein.

extract_protocol: Trizol and Qiagen columns were used according to each manufacturer's protocols.

treatment_protocol: Hearts were rapidly frozen after collection. RNA was extracted from homogenized frozen hearts in Trizol pooled from 4 animals. Purification by Qiagen RNeasy columns

tag: RNA from hearts induced to express a mutated, stable form of HIF-1alpha, under tet-off control, for three days.

source_name: Whole heart tissue from hearts maintained on doxycycline

label_protocol: GE Amersham MicroMAX direct labeling kit with incorporation of dUTP-Cy dye into cDNA. Stringency washing according to manufacturer's protocol.

growth_protocol: In vivo cardiac-specific tet-off control of transgene expression.

tag: RNA from hearts maintained on doxycycline, thereby not expressing the mutated HIF-1alpha.


title: Cardiac response to stable HIF-1alpha overexpression for one day rep 2

label_protocol: GE Amersham MicroMAX direct labeling kit with incorporation of dUTP-Cy dye into cDNA.

tag: RNA from hearts induced to express a mutated, stable form of HIF-1alpha, under tet-off control, for one day.


title: Cardiac response to stable HIF-1alpha overexpression for three days rep 1


title: Pre-transplant sample of DBD patient 06

description:
Gene expression data from DBD donor lungs just after preservation
sample name: No_26


title: Pre-transplant sample of DBD patient 02

description:
Gene expression data from DBD donor lungs just after preservation
sample name: No_22


title: Cardiac response to stable HIF-1alpha overexpression for one day rep 1


title: Pre-transplant sample of DBD patient 03

description:
Gene expression data from DBD donor lungs just after preservation
sample name: No_23


title: Post-transplant sample of DBD patient 01

description:
Gene expression data from DBD donor lungs 2 hours after reperfusion, this is post-transplant data of sample No_21
sample name: No_31


title: Pre-transplant sample of DBD patient 01

description:
Gene expression data from DBD donor lungs just after preservation
sample name: No_21


title: NHGRI_UACC-1529

status: Public on Jan 24 2001

city: Bethesda

first: Michael

last: Bittner

state: MD

department: Cancer Genetics Branch

institute: NHGRI, NIH

description:
Biopsy location: axillary lymph node
Patient sex: male
Patient age: 48
Passage number: 13
p16 mutation status: mutation
Invasive ability: 4.2 +/- 0.5%
Vasculogenic mimicry: +
Gel contraction: -
Cell motility: ND
Scratch wound (%): ND

See http://www.nhgri.nih.gov/DIR/Microarray/Nature_paper.pdf
and http://www.nhgri.nih.gov/DIR/Microarray/Melanoma_Supplement/index.html

source_name: melanoma, lymph node


title: Post-transplant sample of DBD patient 02

description:
Gene expression data from DBD donor lungs 2 hours after reperfusion, this is post-transplant data of sample No_22
sample name: No_32