title: biofluid_ascites_LC_19268654

city: Cheongju

first: Hye Sook

last: Han

address: Chungdae-ro 1, Seowon-gu

country: South Korea

institute: College of Medicine, Chungbuk National University

description: liver cirrhosis-associated benign ascites

hyb_protocol: Labeled cRNA was applied microarray (Agilent technologies, 8x60K)  using Agilent’s Gene Expression Hybridization Kit.

scan_protocol: The microarray chip was scanned using Agilent’s DNA microarray  scanner and the raw signal density was acquired from feature Extraction  software.

data_processing: The differential gene expression was classified by Benjamini-Hochberg's  false discovery ratio method. GeneSpring GX12 software. Gene ontology  and KEGG pathway for the differential expressed genes was analyzed  using GeneSpring GX 12 (Agilent Technologies, San Carlos, CA) and  DAVID Bioinformatics tools (National Institute of Allergy and Infectious  Diseases, NIH).

label_protocol: Cyanine 3-labeled cRNA (complementary RNA) was generated from  Agilent’s Low RNA Input Linear Amplification kit with 500ng total RNA.  Labeled cRNA was also measured using Nanodrop spectrophotometer.

extract_protocol: Exosome from biofluid was precipitated using miRCURY Exosome  Isolation Kit (Exiqon). Exosomal RNA was isolated using Trizol LS  Reagent (Molecular Research Center, Cincinnati, OH, USA), according to  the manufacturer’s instructions. Total RNA was quantified by absorbance  at 260 nm and the integrity of small RNA was checked by  Bioanalyzer2100 (Agilent, CA, USA).

tag: sample

value: ascites

tag: gender

tag: age (years)


title: liver_vehicle_Tuebingen_female_2

status: Public on Feb 12 2019

type: RNA

city: Portland

first: Kim

middle: H

last: Brown

state: OR

address: 1719 SW 10th Ave

department: Department of Biology

country: USA

institute: Portland State University

description: mRNA expression of Tuebingen female following acetone vehicle exposure

hyb_protocol: Samples with total cRNA yields greater than 1.65 µg and specific activity greater than 6 pmol Cy3/µg were fragmented, hybridized to array slides at 65°C for 17 hours, washed briefly, and scanned on an Agilent SureScan array scanner.

scan_protocol: Data were extracted from raw TIFF files using Agilent FeatureExtraction software with grid file 026437_D_F_20140627 and scan protcol AgilentHD_GX_1Color.

data_processing: The cyclic loess method was used for inter-array normalization and replicate probes were averaged within arrays using the limma R package (Ritchie et al. 2015).

molecule: total RNA

organism: Danio rerio

source_name: vehicle-exposed liver, Tuebingen, female, individual 2

label_protocol: Commercially available 4x44K zebrafish mRNA expression arrays, RNA spike-in kit, and Low Input Quick Amp one-color labeling kit (Agilent) were used following manufacturer’s protocols. In brief, cDNA was synthesized from RNA and transcribed into cRNA using Cyanine-3 fluorescent dCTP. Labeled cRNA was purified using a Qiagen RNeasy mini kit per the manufacturer’s protocol and quantified on a NanoDrop spectrophotometer.

extract_protocol: Liver tissue was disrupted with a mortar and pestle prior to homogenization by passing the samples through a nuclease-free syringe and needle in beta-mercaptoethanol lysis buffer. Total RNA was extracted on Qiagen RNeasy columns per the manufacturer's protocol. Nucleic acid concentrations were determined on a Nanodrop Spectrophotometer 2000 and exhibited high 260/280 ratios of 2.09 ± 0.02, respectively (average ± SD).

treatment_protocol: All samples were collected from adult zebrafish housed within the Brown lab zebrafish facility for 2 generations. AB, Tuebingen, and WIK zebrafish were originally sourced from ZIRC (Eugene, OR) as batches of 100 embryos. Livers were extracted from adults aged 12-18 months within 30 minutes of ice water euthanasia and immediately frozen in liquid nitrogen. Individuals were sexed via visual identification of eggs or testes at the time of dissection.

tag: strain

value: Tuebingen

tag: Sex

value: female

tag: treatment

value: 20 ppm (v/v) acetone vehicle for 24 hours

tag: age

value: adult


title: biofluid_ascites_LC_19372249

organism: Homo sapiens

value: male


title: muscle_AB_naïve_female_vs_Casper_2

type: genomic

description:
AB naïve female vs Casper, replicate 2
AB_cont_M

hyb_protocol: Samples with specific activity between 20-60 (where [dye pmol/uL]/[DNA ng/uL]*1000 = specific activity) were fragmented, hybridized to array slides at 65°C for 40 hours, washed briefly, and scanned on an Agilent SureScan array scanner.

scan_protocol: Data were extracted from raw TIFF files using Agilent FeatureExtraction software with grid file 049635_D_F_20130524 and scan protcol CGH_1100_Jul11.

data_processing: Normalized, background subtracted log10 ratios of processed Red signal/processed Green signal (strain/Casper) via Agilent FeatureExtraction software.  Processed data files contain Log10 ratios of processed Red signal/processed Green signal (test/reference = strain/Casper)

molecule: genomic DNA

source_name: white muscle from adult AB female

label_protocol: Custom designed 1x1M zebrafish whole genome CGH arrays, BioPrime array CGH labeling kit (Invitrogen), and Cyanine-3/5 labeled dCTP (Perkin Elmer) were used following manufacturer’s protocols. AB, Tuebingen, and WIK were labeled with Cy-5 and Caseper was labeled with Cy-3. Labeled DNA was purified on Amicon Ultra (Millipore) columns with 0.1X SSC per the manufacturer’s protocol and quantified on a NanoDrop spectrophotometer.

extract_protocol: Muscle tissue was collected from adult zebrafish housed within the Brown lab zebrafish facility for 2 generations. AB, Tuebingen, and WIK zebrafish were originally sourced from ZIRC (Eugene, OR) as batches of 100 embryos. Individuals were sexed via visual identification of eggs or testes at the time of dissection. Pooled Casper samples were collected from 30 homogenized whole fish. Total DNA was extracted on Qiagen DNeasy columns per the manufacturer's protocol. Nucleic acid concentrations were determined on a Nanodrop Spectrophotometer 2000.

tag: tissue

value: white muscle

value: AB

value: naive

source_name: whole body homogenate from pooled Casper adults

value: Casper

value: whole body homogenate


title: cotyledon_24h_28C_rep1

city: Halle

first: Carolin

last: Delker

address: Betty-Heimann-Str. 5

department: Crop Physiology

country: Germany

institute: Martin Luther University Halle

hyb_protocol: Labeled samples were hybridized to the Genechip ATH1 Genome Array (Affymetrix, Santa Clara, CA) according to the manufacturer's instructions (Affymetrix; www.affymetrix.com/)

scan_protocol: The GeneArrays were scanned using the Affymetrix GeneChip Scanner (Affymetrix) using standard NASC protocols.

data_processing: Raw data was processed with the simpleaffy R package to obtain robust multi-chip average (RMA)- normalized log2 expression levels using default settings. The eBayes function of the limma R package was used to compute log fold changes (LFCs), t-values, P values and to correct P values for multiple testing.

label: Biotin

organism: Arabidopsis thaliana

source_name: cotyledons treatment_28°C_24h

label_protocol: Biotinylated cRNA were prepared according to the standard Affymetrix protocol (GeneChip® Expression Analysis Technical Manual, 2001, Affymetrix).

growth_protocol: 5d 20°C, long day (16hL/8hD), 120 µmol/m2/sec PAR from white light LEDs

extract_protocol: Qiagen RNeasy Plant Mini Kit + on-column DNase treatment

treatment_protocol: seedlings were shifted at ZT7 on day 5 and harvested at ZT10 for 3h temperature treatments or at ZT7 on day 6 for 24h treatments. Control plants remained at 20°C but were harvested at the same time.

tag: germplasm/accession

tag: temperature

tag: timepoint

value: cotyledons


title: liver_vehicle_Tuebingen_female_1

source_name: vehicle-exposed liver, Tuebingen, female, individual 1


title: cotyledon_3h_28C_rep2

source_name: cotyledons treatment_28°C_3h


title: muscle_AB_naïve_female_vs_Casper_1

description:
AB naïve female vs Casper, replicate 1
AB_cont_K


title: cotyledon_3h_28C_rep1


title: biofluid_ascites_LC_19506175


title: biofluid_ascites_LC_19503626


title: muscle_AB_naïve_male_vs_Casper_3

description:
AB naïve male vs Casper, replicate 3
AB_cont_I

source_name: white muscle from adult AB male


title: liver_PCB_Tuebingen_female_2

description: mRNA expression of Tuebingen female following PCB exposure

source_name: PCB-exposed liver, Tuebingen, female, individual 2

value: 130 ng/L PCB-126 for 24 hours


title: biofluid_ascites_LC_19503244


title: liver_PCB_Tuebingen_female_1

description: mRNA expression of Tubingen female following PCB exposure

source_name: PCB-exposed liver, Tuebingen, female, individual 1


title: liver_PCB_Tuebingen_female_3

source_name: PCB-exposed liver, Tuebingen, female, individual 3


title: cotyledon_24h_20C_rep2

source_name: cotyledons control_20°C_24h


title: cotyledon_24h_20C_rep3


title: cotyledon_24h_20C_rep1


title: cotyledon_3h_20C_rep3

source_name: cotyledons control_20°C_3h


title: cotyledon_3h_20C_rep2


title: liver_vehicle_AB_male_3

description: mRNA expression of AB male following acetone vehicle exposure

source_name: vehicle-exposed liver, AB, male, individual 3


title: muscle_AB_naïve_male_vs_Casper_2

description:
AB naïve male vs Casper, replicate 2
AB_cont_H


title: liver_vehicle_AB_male_2

source_name: vehicle-exposed liver, AB, male, individual 2


title: muscle_AB_naïve_male_vs_Casper_1

description:
AB naïve male vs Casper, replicate 1
AB_cont_F


title: biofluid_ascites_LC_19502798


title: liver_vehicle_AB_male_1

source_name: vehicle-exposed liver, AB, male, individual 1


title: biofluid_ascites_LC_19503252


title: biofluid_ascites_LC_19504714


title: biofluid_ascites_LC_19502806


title: biofluid_ascites_LC_19503943


title: biofluid_ascites_GC_19637284

description: gastric cancer-associated malignant ascites containing adenocarcinoma cells


title: cotyledon_3h_20C_rep1


title: liver_PCB_AB_male_1

description: mRNA expression of AB male following PCB exposure

source_name: PCB-exposed liver, AB, male, individual 1


title: hypocotyl_24h_28C_rep2

source_name: hypocotyl treatment_28°C_24h

value: hypocotyl


title: biofluid_ascites_GC_19448821


title: biofluid_ascites_GC_19504512


title: liver_PCB_AB_male_3

source_name: PCB-exposed liver, AB, male, individual 3


title: hypocotyl_24h_28C_rep1


title: biofluid_ascites_GC_19504569


title: hypocotyl_24h_28C_rep3


title: liver_vehicle_AB_female_3

description: mRNA expression of AB female following acetone vehicle exposure

source_name: vehicle-exposed liver, AB, female, individual 3


title: Resolving Splenic Treg rep3

city: Chapel Hill

first: Jason

middle: Robert

last: Mock

state: North Carolina

address: 125 Mason Farm Rd, Marsico Hall 7229G

department: Internal Medicine

institute: University of North Carolina

description:
Treg Spleen3
mRNA expression data from mouse splenic regulatory T cells.

hyb_protocol: Fragmented and labeled cDNA was used to prepare a hybridization cocktail with the Affymetrix GeneTitan Hybridization Wash and Stain Kit for WT Arrays. Hybridization, washing, staining and scanning of the Affymetrix peg plate arrays was carried out using the Affymetrix GeneTitan MC Instrument. GeneChip Command Console Software (AGCC) was used for GeneTitan Instrument control.

scan_protocol: Scanning of the Affymetrix peg plate arrays was carried out using the Affymetrix GeneTitan MC Instrument. GeneChip Command Console Software (AGCC) was used for GeneTitan Instrument control.

data_processing:
Affymetrix Expression Console Software was used for basic data analysis and quality control. Microarray data were preprocessed by RMA (Robust Multiarray Average) background correction, GC content and sequence correction, quantile normalization, and median polish summarization, and expression levels compared using ANOVA on log2 intensities to identify transcripts that were differentially expressed between genotype and treatment groups. Differentially expressed (DE) mRNAs were filtered at Benjamini-Hochberg FDR < 0.1, and fold change > 1.5, and expression patterns of DE transcripts were visualized using hierarchical clustering. Expression analyses were performed using Partek Genomics Suite (Partek Inc., St. Louis. MO, USA). Gene Set Enrichment Analysis (GSEA) was performed to identify significant Gene Ontology (GO) biological processes.
Custom gene annotation, or alternative mapping of Affymetrix probesets, based on Affymetrix annotation version NA35 and ENSEMBL gene annotation v81 was used.

label: biotin

organism: Mus musculus

source_name: Resolving Splenic Tregs

label_protocol: Total RNA (225 ng) was used to synthesize fragmented and labeled sense-strand cDNA and hybridize onto Affymetrix arrays. The Affymetrix HT WT User Manual was followed to prepare the samples. Briefly, the WT Expression HT Kit for Robotics (Ambion) was used to generate sense-strand cDNA from total RNA. Following synthesis of sense-strand cDNA, the cDNA was fragmented and labeled with the Affymetrix GeneChip HT Terminal Labeling Kit. The Beckman Coulter Biomek FXP Laboratory Automation Workstation with the Target Express set up was used to prepare the samples with these two kits.

extract_protocol: Total RNA was isolated using Zymo Direct-zol RNA MiniPrep with TRI Reagent miRNeasy kits from Zymo Research (Irvine, CA, USA) according to the manufacturer's instruction with the following modifications: 1) snap-frozen cells were lysed in at least 125 uL of TRI reagent without thawing cells; 2) cells were not centrifuged to collect debris or DNaseI-treated before applying to column. All samples were eluted in 25 uL of DNase/RNase-Free water.

treatment_protocol: Anesthetized mice, Foxp3EGFP (C57BL/6 background), received Escherichia coli LPS O55:B5 (3mg/kg) instilled intratracheally into the lungs. Lymphocytes from single cell suspension from either the lung or spleen were enriched by Percoll density centrifugation (Sigma-Aldrich, St. Louis, MO) and CD4+ GFP+ Tregs then were sorted using a FACSAria (Becton Dickinson, San Jose, CA). Briefly, single cell suspensions were prepared from enzymatically digested and dissected mouse lungs or mechanical dissociation of mouse spleens. The cells were washed with PBS containing 2 mM EDTA and 1.5% bovine serum albumin (FACS buffer). The cells were filtered through a 40 mM filter, and then the filter samples were centrifuged in a 33% Percoll gradient at 2,000 rpm with slow acceleration and slow deceleration to pellet the lymphocyte population. The lymphocyte population were resuspended in FACS buffer at a concentration of 2 x 10e7 cells/mL before then cell sorting CD4+ GFP+ cells. Sorted cells were collected for mRNA analysis. Cells were kept at 4-8°C during staining and sorting.

tag: strain/background

tag: genotype/variation

value: 8-12 weeks

tag: cell type

value: Splenic Regulatory T Cells

value: lipopolysaccharide-induced lung injury


title: hypocotyl_3h_28C_rep2

source_name: hypocotyl treatment_28°C_3h


title: liver_vehicle_AB_female_2

source_name: vehicle-exposed liver, AB, female, individual 2


title: Resolving Splenic Treg rep1

description:
Treg Spleen1
mRNA expression data from mouse splenic regulatory T cells.


title: liver_vehicle_AB_female_1

source_name: vehicle-exposed liver, AB, female, individual 1


title: biofluid_ascites_GC_19504448

value: ascites


title: hypocotyl_3h_28C_rep3


title: Resolving Splenic Treg rep2


title: hypocotyl_3h_28C_rep1


title: Resolving Lung Treg rep3

description:
Treg Lung3
mRNA expression data from mouse lung regulatory T cells.

source_name: Resolving Lung Tregs

value: Lung Regulatory T Cells


title: liver_PCB_AB_male_2

source_name: PCB-exposed liver, AB, male, individual 2


title: Resolving Lung Treg rep2

description:
Treg Lung2
mRNA expression data from mouse lung regulatory T cells.


title: hypocotyl_24h_20C_rep3

source_name: hypocotyl control_20°C_24h


title: hypocotyl_24h_20C_rep2


title: Control Lung Treg rep3

description:
Treg Control3
mRNA expression data from mouse lung regulatory T cells.

source_name: Control Lung Tregs

value: uninjured


title: liver_PCB_AB_female_1

description: mRNA expression of AB female following PCB exposure

source_name: PCB-exposed liver, AB, female, individual 1

value: female


title: Resolving Lung Treg rep1

description:
Treg Lung1
mRNA expression data from mouse lung regulatory T cells.


title: biofluid_ascites_GC_19503174


title: hypocotyl_24h_20C_rep1


title: biofluid_ascites_GC_19501116


title: biofluid_ascites_GC_19508939


title: liver_PCB_AB_female_3

source_name: PCB-exposed liver, AB, female, individual 3

value: adult


title: age-1(hx546); gas-1(fc21) 2

type: SRA

city: Bonn

first: Daniele

last: Bano

address: Sigmund-Freud-Strasse 27

institute: DZNE

library_source: transcriptomic

data_processing:
Demultiplexing of the sequencing reads was performed with Illumina CASAVA (1.8.2). Adapters were trimmed with Skewer (version 0.1.116) (Jiang et al. 2014).
Trimmed raw reads were aligned to the C. elegans reference genome (PRJNA13758) using STAR (version 2.4.0h) (Dobin et al. 2013).
Further analyses were performed with the Cufflinks Tool Suite (Version 2.1.1) (Roberts et al. 2011; Trapnell et al. 2010): Cufflinks was used to count mapped reads.
FPKM values were computed with Cuffdiff (Trapnell et al. 2013) using the “pooled-variance“ model, “geometric“ normalization and “multi-read-correct“ option.
The quality of fastQ files was analyzed with FASTQC (Version 0.10.1) (Andrews 2010).
Genome_build: PRJNA13758
Supplementary_files_format_and_content: excel files including FPKM values for each sample

organism: Caenorhabditis elegans

source_name: whole body

growth_protocol: Nematodes were synchronized via hypochlorite solution treatment and grown at 20°C on standard NGM plates and fed with OP50 E. coli bacteria

extract_protocol:
L4 stage nematodes were collected in M9 buffer, washed 3x and stored at -80°C until needed. RNA was extracted with an RNeasy kit (QIAGEN) as per the manufacturer's instructions
approximately 100 ng of total RNA was used for library construction (TruSeq Stranded mRNA library Prep Kit, Illumina). Sequencing was performed with a HiSeq4000 HighOutput Mode Illumina platform (1x50 bp).

tag: Stage


title: age-1(hx546); gas-1(fc21) 4


title: age-1(hx546); gas-1(fc21) 5


title: age-1(hx546); gas-1(fc21) 3


title: biofluid_ascites_GC_19501344F

value: male


title: biofluid_ascites_GC_19502189


title: Control Lung Treg rep2

description:
Treg Control2
mRNA expression data from mouse lung regulatory T cells.


title: hypocotyl_3h_20C_rep3

source_name: hypocotyl control_20°C_3h


title: age-1(hx546); gas-1(fc21) 1


title: hypocotyl_3h_20C_rep2


title: biofluid_ascites_GC_19502133


title: Control Lung Treg rep1

description:
Treg Control1
mRNA expression data from mouse lung regulatory T cells.


title: hypocotyl_3h_20C_rep1


title: Tph1KO 3 [12 weeks]

city: Ribeirao Preto

first: Vinicius

last: Kannen

state: SP

address: Av Café, s/ N°

department: DACTB

country: Brazil

institute: Universidade de São Paulo

description: Test

data_processing:
The normalization and all the data analysis were performed according to the manufacturers instructions using their web-based software package: https://dataanalysis.qiagen.com/pcr/arrayanalysis
For the normalization it uses the average of five housekeeping genes: Actb, B2m, Gapdh, Gusb, Hsp90ab1
Target gene signals normalized to housekeeping genes; 2^-deltaCt, where deltaCt = (Ct_Target − Ct_HKG)]
The web-based software package automatically performs all deltadeltaCt based fold-change calculations from the uploaded raw threshold cycle data.
Matrix normalized worksheet reports normalized signal (against housekeeping genes). The Qiagen website https://dataanalysis.qiagen.com/pcr/arrayanalysis) only provides the tested samples but not for the control group.
Fold Change worksheet reports test/control ratios.

label: SYBR Green

source_name: Colon samples

label_protocol: PCR assays were performed using the RT² Profiler™ PCR Array Mouse DNA Damage Signaling Pathway (PAMM-029Z; Qiagen, DE) following the Manufacturer’s instructions. Reverse transcription was performed from 500 ng total RNA using the RT2 First Strand Kit (SA Biosciences). Quantitative real-time PCR were performed (Light Cycler 480, Roche) with 45 cycles at 94 oC for 15 seconds and 60 oC for 60 seconds.

extract_protocol: Total RNA was extracted with Qiagen RNeasy Mini Kit .

value: Colon

value: knockout for Tph1

tag: activation agent

value: AOM-exposed samples

tag: time

value: 12 weeks


title: biofluid_ascites_GC_19501608F

tag: sample


title: gas-1(fc21) 5


title: E8.5_Myocardium_Sox17_KO_4

city: Kyoto

first: Rie

last: Saba

state: Kyoto

address: Kajii-cho 465, Kawaramachi-dori, Kamigyo-ku

department: Dapartment of Radiology

country: Japan

institute: Kyoto Prefectural University of Medicine

description: Single cell cDNA

hyb_protocol: According to manufacture's protocol.

scan_protocol: According to manufacture's protocol.

data_processing: Gene Spring GX, ver.13.

source_name: Myocardium

label_protocol: According to manufacture's protocol.

growth_protocol: Crossing Mesp1Cre/+ male and Sox17flox/flox female mice, and dissecting out embryos at 8.5 d.p.c.

extract_protocol:
Embryos were dissected in DMEM (Gibco), the heart tube was then dissociated into single cells by incubation with 0.05% trypsin/EDTA (Gibco) for 5 min at 37  C. The single cells were suspended in DMEM with 3% BSA (Sigma) and transferred to a non-coated petri dish. Each single cell was subsequently transferred to a reaction tube with a capillary pipette under the stereoscopic microscope for cDNA preparation.
Single cell cDNA synthisis and its amplification from the mouse heart tube was performed as described in Kurimoto et al., 2006. IVT RNAs from amplified single cell cDNAs were transcribed with MEGAScript T3Transcription Kit (Ambion).

treatment_protocol: Genotyping for Mesp1-Cre and floxed Sox17 alleles was preformed with tail of the embryos.

value: Somite 9

value: Heart tube

tag: tissue compartment

value: Myocardium

tag: genotype


title: E8.5_Myocardium_Sox17_WT_4

description: Single cell cDNA


title: Tph1KO 2 [12 weeks]

description: Test


title: E8.5_Myocardium_Sox17_KO_3

description: Single cell cDNA


title: gas-1(fc21) 4


title: E8.5_Myocardium_Sox17_KO_2

description: Single cell cDNA


title: E8.5_Myocardium_Sox17_KO_1

description: Single cell cDNA


title: E8.5_Myocardium_Sox17_WT_3

description: Single cell cDNA


title: E8.5_Myocardium_Sox17_WT_1

description: Single cell cDNA


title: E8.5_Myocardium_Sox17_WT_2

description: Single cell cDNA


title: gas-1(fc21) 3


title: Tph1KO 1 [12 weeks]

description: Test


title: gas-1(fc21) 1


title: WT 5


title: E8.5_Endocardium_Sox17_KO_4

description: Single cell cDNA

data_processing: Gene Spring GX, ver.13. For each data set, measurements of less than 50 were set to 50 for data transformation, per chip normalization to the 50th percentile, and per gene normalization to the median.

source_name: Endocardium

value: Endocardium


title: Tph1WT 1 [12 weeks]

description: Control

value: wildtype for Tph1


title: Tph1WT 2 [12 weeks]

description: Control


title: Tph1KO 2 [72 h]

description: Test

value: IR-exposed samples


title: Tph1WT 3 [12 weeks]

description: Control


title: gas-1(fc21) 2


title: Tph1KO 1 [72 h]

description: Test


title: Tph1WT 2 [72 h]

description: Control


title: WT 3


title: WT_C

city: Bengaluru

first: Shikha

last: Laloraya

state: Karnataka

address: New Biological Science Building

department: Biochemistry

country: India

institute: Indian Instritute of Science

data_processing:
Base calling was performed using illumina bcl2fastq v2.9
Low quality and adapter trimming was performed using trim galore v0.4.3 with default parameters and removed reads shorther than 75bp after trimming.
QC passed reads were mapped to genome using STAR v2.5.3a with default parameters
PCR duplicates were marked and removed using MarkDuplicates tool of Picard toolbox v2.17.5.
Mapped reads were counted using featureCounts v1.6.0
Genome_build: R64-2-1_20150113
Supplementary_files_format_and_content: Excel file Containing the read count matrix

organism: Saccharomyces cerevisiae

source_name: Budding yeast

growth_protocol: Cells were grown overnight in YPD medium at 31 degree centigrade, and subcultured starting at OD600 of 0.2 followed by growth for 2 generations at 31 degrees centigrade.

extract_protocol:
Qiagen RNeasy minikit
NEB Next Ultra RNA Library Prep Kit for Illumina was used to generate the libraries.

treatment_protocol: Grown at 31 degrees centigrade

tag: background

value: W303-1a derivative

tag: growth phase

value: logarithmic phase culture

value: wild type


title: WT_D


title: mcd1-1_A


title: mcd1-1_B


title: WT_B


title: E8.5_Endocardium_Sox17_KO_3

description: Single cell cDNA


title: mcd1-1_C


title: Tph1WT 1 [72 h]

description: Control


title: E8.5_Endocardium_Sox17_KO_1

description: Single cell cDNA


title: E8.5_Endocardium_Sox17_KO_2

description: Single cell cDNA


title: E8.5_Endocardium_Sox17_WT_4

description: Single cell cDNA


title: E8.5_Endocardium_Sox17_WT_3

description: Single cell cDNA


title: WT 1


title: mcd1-1_D


title: E8.5_Endocardium_Sox17_WT_2

description: Single cell cDNA


title: WT 4


title: 2AAR_hiWnt-3

status: Public on Feb 11 2019

city: Frankfurt

first: Henner

middle: F.

last: Farin

address: Paul-Ehrlich-Str. 42-44

department: Institute for Tumor Biology and Experimental Therapy

institute: DKTK/Georg-Speyer-Haus

data_processing:
FastQC Analysis
quality score filtering, poly-A trimming, artifact removal, removal of ‘N’ containing reads and clearing of rRNA contamination using a pipeline provided by the HUSAR platform, DKFZ.
Genomic mapping to Human genome 38 was performed using TopHat2 (version 2.0.14)
The number of reads per gene was determined using HTSeq count and the gencode annotations (release 24)
Unpaired differential analysis was performed using   ‘DESeq2’  (version 1.12.4 for samples 1-12 and 1.18.1 for samples 13-42)
Genome_build: Human genome 38
Supplementary_files_format_and_content: .csv
Supplementary_files_format_and_content: processed data files contain readcounts as well as DESeq analysis results

source_name: colon organoids

growth_protocol: Human colon organoids were cultured as described previously (Sato et al., 2011, Long-term Expansion of Epithelial Organoids From Human Colon, Adenoma, Adenocarcinoma, and Barrett’s Epithelium. Gastroenterology 141, 1762–1772.)

extract_protocol:
RNA was extracted using the Macherey-Nagel NucleoSpin RNA kit.
The Illumina TruSeq Stranded RNA 50 cycle kit was used for library preparation.
Illumina TruSeq RNA

treatment_protocol: WT and APC KO organoids were seeded in their regular growing medium containing 50%Wnt (high Wnt), 10% Wnt (low Wnt) or 0% Wnt (no Wnt) in the presence of constant concentration (20%) of Rspo for 6 days.

tag: mutation status

value: APC 2 20AAR

tag: wnt in medium

value: high

tag: donor


title: 3AAR_hiWnt-3

value: APC 3 20AAR


title: 3AAR_hiWnt-2


title: WT 2


title: 2AAR_hiWnt-2


title: 2AAR_hiWnt-1


title: WT_noWnt-3

value: wt

value: no


title: WT_hiWnt-3


title: WT_noWnt-2


title: WT_hiWnt-2


title: WT_noWnt-1


title: APC_hiWNT-3


title: WT_hiWnt-1


title: APC_hiWNT-2


title: APC_loWNT-3

value: low


title: 3AAR_hiWnt-1


title: APC_loWNT-1


title: APC_noWNT-3


title: APC_noWNT-2


title: WT_hiWNT-1


title: WT_hiWNT-3


title: WT_loWNT-2


title: WT_hiWNT-2


title: WT_noWNT-3


title: WT_loWNT-1


title: WT_noWNT-2


title: E8.5_Endocardium_Sox17_WT_1

description: Single cell cDNA

tag: tissue


title: APC_hiWNT-1


title: Donor3_APC_wo_Wnt

data_processing:
FastQC Analysis
quality score filtering, poly-A trimming, artifact removal, removal of ‘N’ containing reads and clearing of rRNA contamination using a pipeline provided by the HUSAR platform, DKFZ.
Genomic mapping to Human genome 38 was performed using TopHat2 (version 2.0.14)
The number of reads per gene was determined using HTSeq count and the gencode annotations (release 24)
Paired differential analysis was performed using   ‘DESeq2’  (version 1.12.4 for samples 1-12 and 1.18.1 for samples 13-42)
Genome_build: Human genome 38
Supplementary_files_format_and_content: processed data files contain readcounts as well as DESeq analysis results

extract_protocol:
RNA was extracted using the Macherey-Nagel NucleoSpin RNA kit.
The Illumina TruSeq RNA kit was used for library preparation.

treatment_protocol: WT and APC KO organoids were seeded in their regular growing medium before they were kept for 2 days in either complete medium containing 50% Wnt/20% Rspo or complete medium deprived from Wnt and Rspo.


title: Donor3_APC_w_Wnt


title: Donor3_WT_wo_Wnt


title: Donor2_WT_wo_Wnt


title: Donor2_APC_w_Wnt


title: APC_loWNT-2


title: Donor3_WT_w_Wnt


title: Donor1_APC_wo_Wnt


title: Donor2_APC_wo_Wnt


title: Donor1_APC_w_Wnt


title: Donor2_WT_w_Wnt


title: WT_noWNT-1


title: APC_noWNT-1


title: Donor1_WT_w_Wnt


title: WT_loWNT-3


title: WT_A


title: MoD10

city: New York

first: Bic

last: MSKCC

state: NY

address: 1275 York Ave.

institute: Memorial SLoan-Kettering Cancer Center

description:
counts_scaled_DESeq.txt
s_MoD10_2__MoD10_2

data_processing:
CutAdapt version cutadapt-1.6 was used to trim and filter reads. Adapters were trimmed with minimum overlap of 10 bases and reads of less than half original read length were filtered out.
STAR version STAR-STAR_2.5.0a was run using the 2-pass method. First pass was run with the following parameters: --outFilterIntronMotifs RemoveNoncanonicalUnannotated --outSAMattributes All --outSAMunmapped Within. Genome database was then generated using the minimum read length of half desired read length and second pass was run against the new genome file with the same parameters as pass 1.
Expression count matrix was computed using HTSeq version HTSeq-0.5.3. Parameters set were: -m intersection-strict -s no -t exon
Raw count matrix was processed using the R/Bioconductor package DESeq, version 1.6.3, which is used to both normalize the full dataset and analyze differential expression between sample groups.
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited file containing normalized counts

source_name: CCR2+ inflammatory monocytes

extract_protocol:
Lungs were harvested and processed for single cell suspensions using DNase I and collagenase type 4 and mechanical disruption using a gentleMACS Dissociator. CCR2+ inflammatory monocytes were enriched using the negative selection EasySep™ Mouse Monocyte Isolation Kit (STEMCELL Technologies). The enriched cells were then analyzed with a BD FACSAria cell sorter to obtain DAPI-Lin-CDllb+Ly6ChiCCR2GFP cells (Lin- = Ter119-CD3-CD19-NK1.1-CD11c-Ly6G-). RNA was extracted using Trizol LS.
After RiboGreen quantification and quality control by Agilent BioAnalyzer, 500ng of total RNA underwent polyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Kit, catalog # RS-122-2102), with 8 cycles of PCR.

treatment_protocol: Mice were challenged with 10^3 Cryptococcus neoformans strain H99 intratracheally.

tag: strain

value: CCR2-GFP reporter

value: Lung

value: CCR2+ inflammatory monocytes


title: Donor1_WT_wo_Wnt


title: MoNaive

description:
counts_scaled_DESeq.txt
s_MoNaive_2__MoNaive_2


title: Spleen_CD69pos_2

city: Berlin

first: Pawel

last: Durek

address: Charitéplatz 1

institute: Deutsches Rheuma-Forschungszentrum

description:
Q_S15
CD69+ LCMV-specific CD4 memory T cells, isolated from spleen of LCMV-primed C57BL/6 mice, 60 days after last immunization

data_processing:
Sequence reads were mapped to mouse GRCm38/mm10 genome with TopHat2 in very-sensitive settings for Bowtie2 and EnsEMBL annotation release 67.
Gene expression was quantified by HTSeq.
Genome_build: mm10
Supplementary_files_format_and_content: Tab-delimited text files including raw counts for genes.

source_name: Isolated from spleen of LCMV-primed C57BL/6 mice, 60 days after last immunization

growth_protocol: Eight week old C57BL/6 male mice were immunized intraperitoneally (i.p.) with 100 μg poly(I:C) and LCMV GP61-80 coupled to murine serum albumin (MSA) and 4-Hydroxy-3-nitrophenylacetyl (NP) . Two weeks after priming, mice were immunized i.p. again with 100 μg poly(I:C) and 100 μg uncoupled LCMV GP61-80.  60 days after the last immunization, cells were isolated from BM and spleen.

extract_protocol:
Total RNA was purified with miRNeasy Micro Kit (Qiagen) from FACS sorted LCMV-specific CD4 memory T cells isolated from spleen and bone marrow. RNA quality was determined on a Bioanalyzer using the RNA 6000 Pico Kit (Agilent).
For poly-A dependent cDNA synthesis and a first amplification step the Smart-Seq v4 mRNA Ultra Low Input RNA Kit (Clontech) was used. After quality control (High sensitivity DNA Kit, Agilent) and concentration measurement (Qubit dsDNA HS Assay Kit, Invitrogen), 150 pg of the purified cDNA was used for tagmentation and library completion with the Nextera XT library preparation kit (Illumina). In the following, 2x75 nucleotides paired-end sequencing was performed on a NextSeq 500 using a mid-output flow cell.

tag: Sex

value: male

tag: age

value: 8 weeks

value: spleen

value: CD69+ LCMV-specific CD4 memory T cells


title: Spleen_CD69pos_3

description:
S_S17
CD69+ LCMV-specific CD4 memory T cells, isolated from spleen of LCMV-primed C57BL/6 mice, 60 days after last immunization


title: MoD5

description:
counts_scaled_DESeq.txt
s_MoD5_7__MoD5_7


title: BM_CD69pos_3

description:
H_S8
CD69+ LCMV-specific CD4 memory T cells, isolated from BM of LCMV-primed C57BL/6 mice, 60 days after last immunization

source_name: Isolated from BM of LCMV-primed C57BL/6 mice, 60 days after last immunization

value: bone marrow


title: Spleen_CD69neg_2

description:
R_S16
CD69- LCMV-specific CD4 memory T cells, isolated from spleen of LCMV-primed C57BL/6 mice, 60 days after last immunization

value: CD69- LCMV-specific CD4 memory T cells


title: BM_CD69pos_2

description:
F_S6
CD69+ LCMV-specific CD4 memory T cells, isolated from BM of LCMV-primed C57BL/6 mice, 60 days after last immunization


title: BM_CD69neg_2

description:
G_S7
CD69- LCMV-specific CD4 memory T cells, isolated from BM of LCMV-primed C57BL/6 mice, 60 days after last immunization


title: Spleen_CD69pos_1

description:
O_S13
CD69+ LCMV-specific CD4 memory T cells, isolated from spleen of LCMV-primed C57BL/6 mice, 60 days after last immunization


title: Spleen_CD69neg_1

description:
P_S14
CD69- LCMV-specific CD4 memory T cells, isolated from spleen of LCMV-primed C57BL/6 mice, 60 days after last immunization


title: BM_CD69neg_3

description:
I_S9
CD69- LCMV-specific CD4 memory T cells, isolated from BM of LCMV-primed C57BL/6 mice, 60 days after last immunization


title: liver_PCB_AB_female_2

source_name: PCB-exposed liver, AB, female, individual 2

value: AB

value: female

tag: treatment

value: 130 ng/L PCB-126 for 24 hours

value: adult


title: CD69- memory CD8 T cells from BM_1

description:
C57Bl6_1_CD8_CD69neg_bm
CD69-CD44+ CD8+ T cells from bone marrow

hyb_protocol: Hybridization was performed in a Hybridization Oven 640, and chips were washed and stained in the Fluidics Station 400 (both Affymetrix).

scan_protocol: Arrays were scanned with an Affymetrix GeneChip Scanner 3000, using the GCOS software version 1.4 from Affymetrix.

data_processing: For quality control data were analyzed using the GeneChip Operating Software (GCOS, Affymetrix), version 1.4. Quality checks were performed according to the manufacturer's recommendations. Gene expression was quantified by RMA using affy R package.

source_name: CD69- memory CD8 T cells from BM

label_protocol: Labeling and hybridization of total RNA to MG 430 2 GeneChips was performed using the whole-transcriptome pico KIT from Affymetrix according tot he manufactures instruction.

growth_protocol: CD69+and CD69- CD3+CD8+CD44+ memory T cells from bone marrow of aged C57BL/6 mice were cytometrically sorted and immediately processed for RNA preparation.

extract_protocol: Total RNA was extracted using the NucleoSpin RNA XS kit (Macherey-Nagel) according to the manufacturer’s recommendations.

tag: cell type

value: memory CD8+ T cells

tag: sorted cell fraction

value: CD69-CD44+ CD8+ T cells


title: BM_CD69neg_1

description:
E_S5
CD69- LCMV-specific CD4 memory T cells, isolated from BM of LCMV-primed C57BL/6 mice, 60 days after last immunization


title: CD69+ memory CD8 T cells from BM_1

description:
C57Bl6_1_CD8_CD69pos_bm
CD69+CD44+ CD8+ T cells from bone marrow

source_name: CD69+ memory CD8 T cells from BM

value: CD69+CD44+ CD8+ T cells


title: CD69- memory CD8 T cells from BM_3

description:
C57Bl6_3_CD8_CD69neg_bm
CD69-CD44+ CD8+ T cells from bone marrow


title: Bach2_nonTFH_2

description: Antigen-specific non-Tfh cells transduced with an inducible Bach2 vector

data_processing:
Sequence reads were mapped to mouse GRCm38/mm10 genome with TopHat2 in very-sensitive settings for Bowtie2 and EnsEMBL annotation release 67.
Gene expression was quantified by featureCounts.
Genome_build: mm10
Supplementary_files_format_and_content: Tab-delimited text files including raw counts for genes.

source_name: Isolated from lymph nodes 6 days after transfer and immunization

growth_protocol: T cells from Smarta TCR-transgenic mice crossed to CreERT2 mice were retrovirally transduced with an inducible Bach2 expression vector or an empty control vector and transferred into C57BL/6 recipients. Five days after s.c. immunization with nitrophenol and GP61-80 coupled to mouse serum albumin, ectopic Bach2 expression was induced by i.p. injection of tamoxifen. After 18 hours, transgenic T cells were isolated from pooled inguinal lymph nodes.

extract_protocol:
RNA was purified with RNeasy Micro Kit (Qiagen) from FACS-sorted antigen-specific (Thy-1.1+) CD44hi CXCR5+ PD-1+ Tfh and CXCR5- PD-1- non-Tfh cells isolated from inguinal lymph nodes. RNA quality was determined on a Bioanalyzer using the RNA 6000 Pico Kit (Agilent).
For library preparation, the Smart-Seq v4 mRNA Ultra Low Input RNA Kit (Clontech) was used in combination with the Nextera XT library preparation kit (Illumina) according to the manufacturer's instructions. Paired-end sequencing with 2x75 nucleotides was performed on a NextSeq500 (Illumina).

value: Antigen-specific non-Tfh cells

tag: transduction

value: inducible vector encoding Bach2

tag: strain (mouse origin)

value: lymph node


title: per2 from RAW264.7 cells infected with B. melitensis M5-90 ∆per for 4h

city: shanghai

first: Xie

last: qin

address: Minchi first road, pujiang town, minhang district, Shanghai

country: China

institute: LC sciences

description: 153 differentially expressed genes were detected in this microarray

hyb_protocol: 0.6 μg of Cy3-labelled cRNA (specific activity >10.0 pmol Cy3/μg cRNA) was fragmented at 60°C for 30 minutes in a reaction volume of 22.5μl containing 1x Agilent fragmentation buffer and 2x Agilent blocking agent following the manufacturers' instructions. On completion of the fragmentation reaction, 22.5μl of 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to Agilent chip for 17 hours at 65°C in a rotating Agilent hybridization oven. After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent) and 1 minute with 37°C GE Wash buffer 2 (Agilent), then dried immediately by brief centrifugation.

scan_protocol: Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505C) using one color scan setting for 4x180k array slides (Scan Area 61x21.6 mm, Scan resolution 3um, Dye channel is set to Green and Green PMT is set to 100%).

data_processing: The scanned images were analyzed with Feature Extraction Software (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities as the raw data. Raw data were normalized in quantile algorithm with Genespring(Agilent).

source_name: RAW264.7 cells

label_protocol: Cy3 labeled cRNA was prepared from 0.2 μg RNA using the One-Color Low RNA Input Linear Amplification PLUS kit (Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA). Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.

extract_protocol: Total RNA was extracted using the Animal Tissue RNA Purification Kit (Norgen Biotek Corporation, Thorold, Canada), following the manufacturer's recommended protocol.

tag: cell line

value: RAW264.7 cells

tag: infection condition

value: B. melitensis M5-90 delta-per

tag: infection time


title: CD69+ memory CD8 T cells from BM_3

description:
C57Bl6_3_CD8_CD69pos_bm
CD69+CD44+ CD8+ T cells from bone marrow


title: CD69+ memory CD8 T cells from BM_2

description:
C57Bl6_2_CD8_CD69pos_bm
CD69+CD44+ CD8+ T cells from bone marrow


title: M5_90_3 from RAW264.7 cells infected with B. melitensis M5-90 for 4h

value: B. melitensis M5-90


title: Spleen_CD69neg_3

description:
18_S18
CD69- LCMV-specific CD4 memory T cells, isolated from spleen of LCMV-primed C57BL/6 mice, 60 days after last immunization

value: male

value: 8 weeks

value: spleen


title: M5_90_2 from RAW264.7 cells infected with B. melitensis M5-90 for 4h


title: per1 from RAW264.7 cells infected with B. melitensis M5-90 ∆per for 4h


title: per3 from RAW264.7 cells infected with B. melitensis M5-90 ∆per for 4h


title: M5_90_1 from RAW264.7 cells infected with B. melitensis M5-90 for 4h


title: Bach2_nonTFH_1


title: CD69- memory CD8 T cells from BM_2

description:
C57Bl6_2_CD8_CD69neg_bm
CD69-CD44+ CD8+ T cells from bone marrow

value: bone marrow


title: Bach2_TFH_1

description: Antigen-specific Tfh cells transduced with an inducible Bach2 vector

value: Antigen-specific Tfh cells


title: EV_nonTFH_1

description: Antigen-specific non-Tfh cells transduced with a empty control vector

value: control


title: T32 non-TFH

source_name: Isolated from lymph nodes 8 days after transfer and immunization

growth_protocol: T cells from OT-II TCR-transgenic mice were transferred into C57BL/6 recipients, which were subsequently immunized s.c. with nitrophenol coupled to ovalbumin.  Transgenic T cells were isolated from pooled inguinal lymph nodes on day 8 after immunization.

extract_protocol:
RNA was purified with the Roche High Pure RNA Isolation Kit from FACS-sorted antigen-specific (Thy-1.1+) CD44hi CXCR5+ PD-1+ Tfh and CXCR5- PD-1- non-Tfh cells isolated from inguinal lymph nodes. RNA quality was determined on a Bioanalyzer using the RNA 6000 Pico Kit (Agilent).
The library was prepared using the TruSeq® Stranded Total RNA Sample Preparation Kit (Illumina) according to the manufacturer's instructions. Paired-end sequencing with 2x75 nucleotides was performed on a NextSeq500 (Illumina).


title: EV_TFH_2

description: Antigen-specific Tfh cells transduced with an empty control vector


title: Bach2_TFH_2


title: PDAC_5

city: Philadelphia

first: Junhyong

last: Kim

state: PA

address: 433 S University Avenue

department: Biology

institute: University of Pennsylvania

data_processing:
miRNA expression was found with mirDeep2 (29) and Bowtie (30) (mm10), using miRBase version 21 (31).
Genome_build: mm10
Supplementary_files_format_and_content: mircoRNA read counts generated by mirdeep2.

source_name: Exosomes

extract_protocol:
miRNeasy kit from Qiagen was used to extract RNA from exosomes.
NEBNext Small RNA Library Prep kit for Illumina from New England BioLabs was used to make library for small RNA sequencing.

tag: sample type

value: Exosomes isolated from plasma

value: Pdx1-cre, KrasLSL-G12D, p53L/+, RosaYFP/YFP (KPCY)


title: PDAC_3


title: BM_CD69pos_1

description:
D_S4
CD69+ LCMV-specific CD4 memory T cells, isolated from BM of LCMV-primed C57BL/6 mice, 60 days after last immunization

value: CD69+ LCMV-specific CD4 memory T cells


title: podocin Cre rep1

city: Birmingham

first: Caroline

middle: B

last: Marshall

state: AL

address: 1720 2nd Ave S

department: Medicine/Nephrology

institute: University of Alabama at Birmingham

data_processing:
Illumina bcl2fastq version 2.18.0.12 software used for basecalling.
Sequenced reads were mapped to Gencode GRCm38 p4 Release M11 (mm10) using STAR version 2.5.4b using parameters --outReadsUnmapped Fastx --outSAMtype BAM SortedByCoordinate --outSAMattributes All --outFilterIntronMotifs RemoveNoncanonicalUnannotated
Transcript abundance was calculated using Cufflinks version 2.2.1 with parameters -G -L
Cuffdiff version 2.2.1 with default parameters was used to determine pairwise differential expression
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited Excel files include FPKM values for each Sample

source_name: podocin Cre rep1

growth_protocol: specific pathogen-free, temperature-controlled environment, 12-hr (0600-1800) light-dark cycle, access to standard chow, water ad libitum

extract_protocol:
following intra-cardiac perfusion, glomeruli were isolated and purified using Dynabeads
RNA library were prepared for sequencing using standard Illumina protocols

treatment_protocol: aged to 24 weeks

value: glomeruli

value: 24 weeks

tag: genotype/variation

value: NPHS2-Cre +/+


title: WT rep1

source_name: WT rep1

value: wildtype


title: podocin Cre rep2

source_name: podocin Cre rep2


title: PDAC_4


title: WT rep2

source_name: WT rep2


title: T53 non-TFH

value: Antigen-specific non-Tfh cells

value: lymph node


title: PanIN_6


title: PDAC_1


title: PDAC_2


title: PanIN_4


title: PanIN_5


title: EV_nonTFH_2


title: PanIN_3


title: PanIN_2


title: PanIN_1


title: T32 TFH


title: T53 TFH


title: Control_1


title: EV_TFH_1


title: ChIP-seq for H3K4me1 from Mouse Embryonic Fibroblasts (WT Trp53)

city: Albany

first: Morgan

middle: A

last: Sammons

address: 1400 Washington Ave, Life Sciences 2075

department: Biological Sciences

institute: University at Albany - SUNY

library_source: genomic

data_processing:
Raw fastq reads were processed for quality control using FastQC v0.11.5
Reads were aligned to the reference genome (hg19) using STAR v 2.5.3a. Genome indices for STAR were built using the parameters --runThreadN 12 --runMode genomeGenerate --sjdbOverhang 100
Indexes built in the aforementioned step were then used to align reads with STAR using the parameters --runThreadN 12 --genomeDir <genomeDir> --readFilesIn <Sample.fastq.gz> --readFilesCommand zcat --outSAMstrandField intronMotif --outFilterIntronMotifsRemoveNoncanonical --outSAMtype BAM SortedByCoordinate --outFileNamePrefix Sample. --limitBAMsortRAM 61675612266
Gene counts were calculated using HTSeq v 0.9.1 with -f bam -s no -I gene_id -m intersection-strict parameters
Genome_build: hg19
Supplementary_files_format_and_content: The count files are tab-delimited text files with first column as geneNames and second column as geneCounts

source_name: Mouse embryonic fibroblasts

growth_protocol: MCF10A epithelial cells were cultured in HuMEC Complete media (Gibco, #12752010) and skin fibroblast cells were cultured  in DMEM media (with 10% FBS and 1% Pen/Strep, Gibco)

extract_protocol:
For RNA-Seq: Total mRNA was extracted with E.Z.N.A. Total RNA kit (Omega; R6834-02) and Poly(A)+ RNA was isolated by double selection with poly-dT beads, using 2.5 mg total RNA, which is then followed by first- and second-strand synthesis
For RNA-Seq: Libraries were prepared using NEXTflex Rapid Illumina DNA-Seq Library Prep Kit (Bioo Scientific).
For ChIP-Seq: Cells were crosslinked at 80% confluency with 1% formaldehyde for 10 min at room temperature. Crosslinking was quenched with 125mM glycine and the resulting pellet was washed twice with cold PBS and lysed. Samples were subjected to sonication with Diagenode Bioruptor Plus for 40 cycles (30 sec on/off at high setting) for shearing chromatin to 150-500 bp average size. Immunoprecipitation reactions were performed with Diagenode IP-Star Compact Atuomated System with mentioned antibodies. Immunoprecipitated DNA was reverse-crosslinked at 65°C for 4 hours and then eluted from beads, purified using SPRI beads, and used to construct sequencing libraries.
For ChIP-Seq: Libraries were prepared using NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs).

treatment_protocol: To induce p53 activation, cells were treated with Nutlin-3A (5 mM final) or DMSO (vehicle control) for 6 hours.

tag: antibody


title: Control_2


title: ChIP-seq for H3K4me2 from Mouse Embryonic Fibroblasts (WT Trp53)


title: Input for ChIP-seq from Mouse Embryonic Fibroblasts (WT Trp53)

value: Input


title: Input for ChIP-seq from Mouse Embryonic Fibroblasts (Trp53-null)


title: TR4PL43_15C

city: Oxford

first: Alba

last: Rodriguez-Meira

address: John Radcliffe Hospital/Headley Way

department: Weatherall Institute of Molecular Medicine

country: United Kingdom

institute: University of Oxford

description: Rodriguez-Meira_et.al_3TARGET_counts.txt

data_processing:
BCL files were demultiplexed into FASTQ files using bcl2fastq, using the first 8 bp obtained from R1, corresponding to a cell-specific barcode.
RNA-Seq:Short reads (63-bp) were trimmed for polyA tails and aligned to the human genome using STAR
RNA-Seq: primary alignments mapping to a set of RefSeq annotated transcripts were quantified using FeatureCounts
RNA-Seq: Expression values were quantified as counts and normalized per library size and a scale factor (mean library size:68,412)
Genome_build: GRCh37 assembly (hg19)
Supplementary_files_format_and_content: Normalized counts

molecule: polyA RNA

source_name: TR4PL43_15C_Peripheral blood_JAK2_HET

extract_protocol:
cDNA libraries were synthetized from single cells using 3'-TARGET-seq method
Illumina sequencing libraries were prepared using the Illumina Nextera XT DNA kit, using a custom P5 primer for PCR amplification. Samples were sequenced on Illumina NextSeq Instrument using a custom primer for Read1 (cell barcode)

treatment_protocol: HSPC (defined as Lineage-CD34+ cells) were isolated by Fluorescent Activated Cell Sorting from patients or normal donors

tag: subject id

value: cd34+ HSPC

value: Peripheral blood

tag: method

tag: disease timepoint

value: baseline

tag: diagnosis

value: myelofibrosis

tag: donor type

value: patient

tag: primer mix

tag: batch

tag: plate

tag: mutation

tag: multimap

value: realclone


title: TR4PL43_16A

source_name: TR4PL43_16A_Peripheral blood_JAK2_HET

tag: cell type

tag: tissue


title: TR4PL43_16I

source_name: TR4PL43_16I_Peripheral blood_JAK2_HET


title: TR4PL43_15G

source_name: TR4PL43_15G_Peripheral blood_JAK2_HET


title: TR4PL43_15K

source_name: TR4PL43_15K_Peripheral blood_JAK2_HET


title: TR4PL43_16G

source_name: TR4PL43_16G_Peripheral blood_JAK2_HET


title: TR4PL43_15I

source_name: TR4PL43_15I_Peripheral blood_JAK2_WT


title: TR4PL43_16E

source_name: TR4PL43_16E_Peripheral blood_JAK2_HET


title: TR4PL43_16C

source_name: TR4PL43_16C_Peripheral blood_JAK2_WT


title: TR4PL43_15E

source_name: TR4PL43_15E_Peripheral blood_JAK2_WT


title: ChIP-seq for H3K4me1 from Mouse Embryonic Fibroblasts (Trp53-null)

tag: treatment


title: TR4PL43_15A

source_name: TR4PL43_15A_Peripheral blood_JAK2_WT


title: TR4PL43_14K

source_name: TR4PL43_14K_Peripheral blood_JAK2_WT


title: TR4PL43_14A

source_name: TR4PL43_14A_Peripheral blood_JAK2_HET


title: TR4PL43_14C

source_name: TR4PL43_14C_Peripheral blood_JAK2_WT


title: TR4PL43_13K

source_name: TR4PL43_13K_Peripheral blood_JAK2_HET


title: TR4PL43_13G

source_name: TR4PL43_13G_Peripheral blood_JAK2_WT


title: TR4PL43_13I

source_name: TR4PL43_13I_Peripheral blood_JAK2_WT


title: TR4PL43_14I

source_name: TR4PL43_14I_Peripheral blood_JAK2_HET


title: ChIP-seq for H3K4me2 from Mouse Embryonic Fibroblasts (Trp53-null)

source_name: Mouse embryonic fibroblasts


title: TR4PL43_13E

source_name: TR4PL43_13E_Peripheral blood_JAK2_HET


title: TR4PL43_13C

source_name: TR4PL43_13C_Peripheral blood_JAK2_HET


title: TR4PL43_12I

source_name: TR4PL43_12I_Peripheral blood_JAK2_HET


title: TR4PL43_13A

source_name: TR4PL43_13A_Peripheral blood_JAK2_WT


title: TR4PL43_12E

source_name: TR4PL43_12E_Peripheral blood_JAK2_WT


title: TR4PL43_12G

source_name: TR4PL43_12G_Peripheral blood_JAK2_WT


title: TR4PL43_12K

source_name: TR4PL43_12K_Peripheral blood_JAK2_WT


title: TR4PL43_14M

source_name: TR4PL43_14M_Peripheral blood_JAK2_HET


title: TR4PL43_14E

source_name: TR4PL43_14E_Peripheral blood_JAK2_WT


title: TR4PL43_12C

source_name: TR4PL43_12C_Peripheral blood_JAK2_HET


title: TR4PL43_11K

source_name: TR4PL43_11K_Peripheral blood_JAK2_WT


title: TR4PL43_11M

source_name: TR4PL43_11M_Peripheral blood_JAK2_HET


title: TR4PL43_11G

source_name: TR4PL43_11G_Peripheral blood_JAK2_HET


title: TR4PL43_13M

source_name: TR4PL43_13M_Peripheral blood_JAK2_HET


title: TR4PL43_11A

source_name: TR4PL43_11A_Peripheral blood_JAK2_HET


title: TR4PL43_10G

source_name: TR4PL43_10G_Peripheral blood_JAK2_HET


title: TR4PL43_10C

source_name: TR4PL43_10C_Peripheral blood_JAK2_HET


title: TR4PL40_9J

source_name: TR4PL40_9J_Peripheral blood_JAK2_HOM


title: TR4PL43_10A

source_name: TR4PL43_10A_Peripheral blood_JAK2_WT


title: TR4PL40_9O

source_name: TR4PL40_9O_Peripheral blood_JAK2_HOM


title: TR4PL40_9C

source_name: TR4PL40_9C_Peripheral blood_JAK2_HOM


title: TR4PL40_7M

source_name: TR4PL40_7M_Peripheral blood_JAK2_HOM


title: TR4PL43_11C

source_name: TR4PL43_11C_Peripheral blood_JAK2_HET


title: TR4PL40_9M


title: TR4PL40_7K

source_name: TR4PL40_7K_Peripheral blood_JAK2_HOM


title: TR4PL40_9D

source_name: TR4PL40_9D_Peripheral blood_JAK2_HOM


title: TR4PL43_11E

source_name: TR4PL43_11E_Peripheral blood_JAK2_WT


title: TR4PL40_7L

source_name: TR4PL40_7L_Peripheral blood_JAK2_HOM


title: TR4PL40_9I

source_name: TR4PL40_9I_Peripheral blood_JAK2_HOM


title: TR4PL40_7I

source_name: TR4PL40_7I_Peripheral blood_JAK2_HOM


title: TR4PL40_5P

source_name: TR4PL40_5P_Peripheral blood_JAK2_HOM


title: TR4PL40_5M

source_name: TR4PL40_5M_Peripheral blood_JAK2_HOM


title: TR4PL40_5O

source_name: TR4PL40_5O_Peripheral blood_JAK2_HOM


title: TR4PL40_3P

source_name: TR4PL40_3P_Peripheral blood_JAK2_HOM


title: TR4PL40_7G

source_name: TR4PL40_7G_Peripheral blood_JAK2_HOM


title: TR4PL40_3M

source_name: TR4PL40_3M_Peripheral blood_JAK2_HOM


title: TR4PL43_11I

source_name: TR4PL43_11I_Peripheral blood_JAK2_HET


title: TR4PL43_12A

source_name: TR4PL43_12A_Peripheral blood_JAK2_HET


title: TR4PL40_3N

source_name: TR4PL40_3N_Peripheral blood_JAK2_HOM


title: TR4PL40_3L

source_name: TR4PL40_3L_Peripheral blood_JAK2_HOM


title: TR4PL40_3K

source_name: TR4PL40_3K_Peripheral blood_JAK2_HOM


title: TR4PL40_3J

value: Undetermined


title: TR4PL40_3I

source_name: TR4PL40_3I_Peripheral blood_JAK2_HOM


title: TR4PL40_3G

source_name: TR4PL40_3G_Peripheral blood_JAK2_HOM


title: TR4PL40_3C

source_name: TR4PL40_3C_Peripheral blood_JAK2_HOM


title: TR4PL40_3D

source_name: TR4PL40_3D_Peripheral blood_JAK2_HOM


title: TR4PL40_23K

source_name: TR4PL40_23K_Peripheral blood_JAK2_HOM


title: TR4PL40_23L

source_name: TR4PL40_23L_Peripheral blood_JAK2_HOM


title: TR4PL40_23M

source_name: TR4PL40_23M_Peripheral blood_JAK2_HOM


title: TR4PL40_3B

source_name: TR4PL40_3B_Peripheral blood_JAK2_HOM


title: TR4PL40_23F

source_name: TR4PL40_23F_Peripheral blood_JAK2_HOM


title: TR4PL40_3A

source_name: TR4PL40_3A_Peripheral blood_JAK2_HOM


title: TR4PL40_23G

source_name: TR4PL40_23G_Peripheral blood_JAK2_HOM


title: TR4PL40_3H

source_name: TR4PL40_3H_Peripheral blood_JAK2_HOM


title: TR4PL40_23E

source_name: TR4PL40_23E_Peripheral blood_JAK2_HOM


title: TR4PL40_3E

source_name: TR4PL40_3E_Peripheral blood_JAK2_HOM


title: TR4PL40_23C


title: TR4PL40_23H

source_name: TR4PL40_23H_Peripheral blood_JAK2_HOM


title: TR4PL40_23B

source_name: TR4PL40_23B_Peripheral blood_JAK2_HOM


title: TR4PL40_23A

source_name: TR4PL40_23A_Peripheral blood_JAK2_HOM


title: TR4PL40_21N

source_name: TR4PL40_21N_Peripheral blood_JAK2_HOM


title: TR4PL40_21M

source_name: TR4PL40_21M_Peripheral blood_JAK2_HOM


title: TR4PL40_23D

source_name: TR4PL40_23D_Peripheral blood_JAK2_HOM


title: TR4PL40_21L

source_name: TR4PL40_21L_Peripheral blood_JAK2_HOM


title: TR4PL40_21O


title: TR4PL40_3F

source_name: TR4PL40_3F_Peripheral blood_JAK2_HOM


title: TR4PL40_21K


title: TR4PL40_23P

source_name: TR4PL40_23P_Peripheral blood_JAK2_HOM


title: TR4PL40_21I

source_name: TR4PL40_21I_Peripheral blood_JAK2_HOM


title: TR4PL40_21G

source_name: TR4PL40_21G_Peripheral blood_JAK2_HOM


title: TR4PL40_21H

source_name: TR4PL40_21H_Peripheral blood_JAK2_HOM


title: TR4PL40_21F

source_name: TR4PL40_21F_Peripheral blood_JAK2_HOM


title: TR4PL40_21E

source_name: TR4PL40_21E_Peripheral blood_JAK2_HOM


title: TR4PL40_19M

source_name: TR4PL40_19M_Peripheral blood_JAK2_WT


title: TR4PL40_19P

source_name: TR4PL40_19P_Peripheral blood_JAK2_HOM


title: TR4PL40_19N

source_name: TR4PL40_19N_Peripheral blood_JAK2_HOM


title: TR4PL40_19J

source_name: TR4PL40_19J_Peripheral blood_JAK2_HOM


title: TR4PL40_21A

source_name: TR4PL40_21A_Peripheral blood_JAK2_HOM


title: TR4PL40_21P

source_name: TR4PL40_21P_Peripheral blood_JAK2_HOM


title: TR4PL40_19L

source_name: TR4PL40_19L_Peripheral blood_JAK2_HOM


title: TR4PL40_19H

source_name: TR4PL40_19H_Peripheral blood_JAK2_HOM


title: TR4PL40_19F

source_name: TR4PL40_19F_Peripheral blood_JAK2_HOM


title: TR4PL40_19O

source_name: TR4PL40_19O_Peripheral blood_JAK2_HOM


title: TR4PL40_19G

source_name: TR4PL40_19G_Peripheral blood_JAK2_HOM


title: TR4PL40_19E

source_name: TR4PL40_19E_Peripheral blood_JAK2_HOM


title: TR4PL40_21J

source_name: TR4PL40_21J_Peripheral blood_JAK2_HOM


title: TR4PL40_19D

source_name: TR4PL40_19D_Peripheral blood_JAK2_HOM


title: TR4PL40_21C

source_name: TR4PL40_21C_Peripheral blood_JAK2_HOM


title: TR4PL40_19K

source_name: TR4PL40_19K_Peripheral blood_JAK2_HOM


title: TR4PL40_17P

source_name: TR4PL40_17P_Peripheral blood_JAK2_HOM


title: TR4PL40_19B

source_name: TR4PL40_19B_Peripheral blood_JAK2_HOM


title: TR4PL40_17M


title: TR4PL40_17N

source_name: TR4PL40_17N_Peripheral blood_JAK2_HOM


title: TR4PL40_19C

source_name: TR4PL40_19C_Peripheral blood_JAK2_HOM


title: TR4PL40_17K

source_name: TR4PL40_17K_Peripheral blood_JAK2_HOM


title: TR4PL40_17O

source_name: TR4PL40_17O_Peripheral blood_JAK2_HOM


title: TR4PL40_17I

source_name: TR4PL40_17I_Peripheral blood_JAK2_HOM


title: TR4PL40_17J

source_name: TR4PL40_17J_Peripheral blood_JAK2_HOM


title: TR4PL40_17H

source_name: TR4PL40_17H_Peripheral blood_JAK2_HOM


title: TR4PL40_17G

source_name: TR4PL40_17G_Peripheral blood_JAK2_HOM


title: TR4PL40_17B

source_name: TR4PL40_17B_Peripheral blood_JAK2_HOM


title: TR4PL40_17E

source_name: TR4PL40_17E_Peripheral blood_JAK2_HOM


title: TR4PL40_15P

source_name: TR4PL40_15P_Peripheral blood_JAK2_HOM


title: TR4PL40_15M

source_name: TR4PL40_15M_Peripheral blood_JAK2_HOM


title: TR4PL40_17A

source_name: TR4PL40_17A_Peripheral blood_JAK2_HOM


title: TR4PL40_17F

source_name: TR4PL40_17F_Peripheral blood_JAK2_WT


title: TR4PL40_15K

source_name: TR4PL40_15K_Peripheral blood_JAK2_HOM


title: TR4PL40_15H

source_name: TR4PL40_15H_Peripheral blood_JAK2_HOM


title: TR4PL40_15J

source_name: TR4PL40_15J_Peripheral blood_JAK2_HOM


title: TR4PL40_15N

source_name: TR4PL40_15N_Peripheral blood_JAK2_HOM


title: TR4PL40_15D

source_name: TR4PL40_15D_Peripheral blood_JAK2_HOM


title: TR4PL40_15G


title: TR4PL40_15A

source_name: TR4PL40_15A_Peripheral blood_JAK2_HOM


title: TR4PL40_13M

source_name: TR4PL40_13M_Peripheral blood_JAK2_HOM


title: TR4PL40_13P

source_name: TR4PL40_13P_Peripheral blood_JAK2_HOM


title: TR4PL40_13H

source_name: TR4PL40_13H_Peripheral blood_JAK2_HOM


title: TR4PL40_13J

source_name: TR4PL40_13J_Peripheral blood_JAK2_HOM


title: TR4PL40_13I

source_name: TR4PL40_13I_Peripheral blood_JAK2_HOM


title: TR4PL40_13N

source_name: TR4PL40_13N_Peripheral blood_JAK2_HOM


title: TR4PL40_13L

source_name: TR4PL40_13L_Peripheral blood_JAK2_HOM


title: TR4PL40_13D

source_name: TR4PL40_13D_Peripheral blood_JAK2_HOM


title: TR4PL40_13F


title: TR4PL40_13G

source_name: TR4PL40_13G_Peripheral blood_JAK2_HOM


title: TR4PL40_13O

source_name: TR4PL40_13O_Peripheral blood_JAK2_HOM


title: TR4PL40_13C

source_name: TR4PL40_13C_Peripheral blood_JAK2_WT


title: TR4PL40_13E

source_name: TR4PL40_13E_Peripheral blood_JAK2_WT


title: TR4PL40_11P

source_name: TR4PL40_11P_Peripheral blood_JAK2_WT


title: TR4PL40_13K


title: TR4PL40_15O

source_name: TR4PL40_15O_Peripheral blood_JAK2_HOM


title: TR4PL40_11N

source_name: TR4PL40_11N_Peripheral blood_JAK2_HOM


title: TR4PL40_11M

source_name: TR4PL40_11M_Peripheral blood_JAK2_HOM


title: TR4PL40_11K

source_name: TR4PL40_11K_Peripheral blood_JAK2_HOM


title: TR4PL40_11O

source_name: TR4PL40_11O_Peripheral blood_JAK2_HOM


title: TR4PL40_11G

source_name: TR4PL40_11G_Peripheral blood_JAK2_HOM


title: TR4PL40_11J


title: TR4PL40_11I

source_name: TR4PL40_11I_Peripheral blood_JAK2_HOM


title: TR4PL40_11H

source_name: TR4PL40_11H_Peripheral blood_JAK2_HOM


title: TR4PL40_11F


title: TR4PL40_11E

source_name: TR4PL40_11E_Peripheral blood_JAK2_HOM


title: TR4PL40_11C

source_name: TR4PL40_11C_Peripheral blood_JAK2_HOM


title: TR4PL40_11B

source_name: TR4PL40_11B_Peripheral blood_JAK2_HOM


title: TR4PL37_9O

source_name: TR4PL37_9O_Peripheral blood_WT

value: healthy control

value: normal

value: WT


title: TR4PL40_11A

source_name: TR4PL40_11A_Peripheral blood_JAK2_HOM


title: TR4PL37_9M

source_name: TR4PL37_9M_Peripheral blood_WT


title: TR4PL37_9G

source_name: TR4PL37_9G_Peripheral blood_WT


title: TR4PL37_9E

source_name: TR4PL37_9E_Peripheral blood_WT


title: TR4PL37_7M

source_name: TR4PL37_7M_Peripheral blood_WT


title: TR4PL37_7K

source_name: TR4PL37_7K_Peripheral blood_WT


title: TR4PL37_7C

source_name: TR4PL37_7C_Peripheral blood_WT


title: TR4PL37_7A

source_name: TR4PL37_7A_Peripheral blood_WT


title: TR4PL37_9C

source_name: TR4PL37_9C_Peripheral blood_WT


title: TR4PL37_5O

source_name: TR4PL37_5O_Peripheral blood_WT


title: TR4PL37_7E

source_name: TR4PL37_7E_Peripheral blood_WT


title: TR4PL37_5M

source_name: TR4PL37_5M_Peripheral blood_WT


title: TR4PL37_5K

source_name: TR4PL37_5K_Peripheral blood_WT


title: TR4PL37_5I

source_name: TR4PL37_5I_Peripheral blood_WT


title: TR4PL37_9I

source_name: TR4PL37_9I_Peripheral blood_WT


title: TR4PL37_5A

source_name: TR4PL37_5A_Peripheral blood_WT


title: TR4PL37_3O

source_name: TR4PL37_3O_Peripheral blood_WT


title: TR4PL37_3E

source_name: TR4PL37_3E_Peripheral blood_WT


title: TR4PL37_3K

source_name: TR4PL37_3K_Peripheral blood_WT


title: TR4PL37_5G

source_name: TR4PL37_5G_Peripheral blood_WT


title: TR4PL37_5E

source_name: TR4PL37_5E_Peripheral blood_WT


title: TR4PL40_15B

source_name: TR4PL40_15B_Peripheral blood_JAK2_HOM


title: TR4PL37_3M

source_name: TR4PL37_3M_Peripheral blood_WT


title: TR4PL37_23O

source_name: TR4PL37_23O_Peripheral blood_WT


title: TR4PL37_7G

source_name: TR4PL37_7G_Peripheral blood_WT


title: TR4PL40_11D

source_name: TR4PL40_11D_Peripheral blood_JAK2_HOM


title: TR4PL37_5C

source_name: TR4PL37_5C_Peripheral blood_WT


title: TR4PL37_3I

source_name: TR4PL37_3I_Peripheral blood_WT


title: TR4PL37_3C

source_name: TR4PL37_3C_Peripheral blood_WT


title: TR4PL37_23K

source_name: TR4PL37_23K_Peripheral blood_WT


title: TR4PL37_23M

source_name: TR4PL37_23M_Peripheral blood_WT


title: TR4PL37_3G

source_name: TR4PL37_3G_Peripheral blood_WT


title: TR4PL37_23I

source_name: TR4PL37_23I_Peripheral blood_WT


title: TR4PL37_3A

source_name: TR4PL37_3A_Peripheral blood_WT


title: TR4PL37_23G

source_name: TR4PL37_23G_Peripheral blood_WT


title: TR4PL37_23E

source_name: TR4PL37_23E_Peripheral blood_WT


title: TR4PL37_23A

source_name: TR4PL37_23A_Peripheral blood_WT


title: TR4PL37_21E

source_name: TR4PL37_21E_Peripheral blood_WT


title: TR4PL37_21I

source_name: TR4PL37_21I_Peripheral blood_WT


title: TR4PL37_21C

source_name: TR4PL37_21C_Peripheral blood_WT


title: TR4PL37_21A

source_name: TR4PL37_21A_Peripheral blood_WT


title: TR4PL37_21O

source_name: TR4PL37_21O_Peripheral blood_WT


title: TR4PL37_21G

source_name: TR4PL37_21G_Peripheral blood_WT


title: TR4PL37_1G

source_name: TR4PL37_1G_Peripheral blood_WT


title: TR4PL37_1E

source_name: TR4PL37_1E_Peripheral blood_WT


title: TR4PL37_1O

source_name: TR4PL37_1O_Peripheral blood_WT


title: TR4PL37_1K

source_name: TR4PL37_1K_Peripheral blood_WT


title: TR4PL37_1M

source_name: TR4PL37_1M_Peripheral blood_WT


title: TR4PL37_1A

source_name: TR4PL37_1A_Peripheral blood_WT


title: TR4PL37_21K

source_name: TR4PL37_21K_Peripheral blood_WT


title: TR4PL37_7I

source_name: TR4PL37_7I_Peripheral blood_WT


title: TR4PL37_19M

source_name: TR4PL37_19M_Peripheral blood_WT


title: TR4PL37_19O

source_name: TR4PL37_19O_Peripheral blood_WT


title: TR4PL37_19K

source_name: TR4PL37_19K_Peripheral blood_WT


title: TR4PL37_19I

source_name: TR4PL37_19I_Peripheral blood_WT


title: TR4PL37_19G

source_name: TR4PL37_19G_Peripheral blood_WT


title: TR4PL37_19C

source_name: TR4PL37_19C_Peripheral blood_WT


title: TR4PL37_21M

source_name: TR4PL37_21M_Peripheral blood_WT


title: TR4PL37_19A

source_name: TR4PL37_19A_Peripheral blood_WT


title: TR4PL37_23C

source_name: TR4PL37_23C_Peripheral blood_WT


title: TR4PL37_19E

source_name: TR4PL37_19E_Peripheral blood_WT


title: TR4PL37_17K

source_name: TR4PL37_17K_Peripheral blood_WT


title: TR4PL37_17I

source_name: TR4PL37_17I_Peripheral blood_WT


title: TR4PL37_17M

source_name: TR4PL37_17M_Peripheral blood_WT


title: TR4PL37_1I

source_name: TR4PL37_1I_Peripheral blood_WT


title: TR4PL37_17E

source_name: TR4PL37_17E_Peripheral blood_WT


title: TR4PL37_17C

source_name: TR4PL37_17C_Peripheral blood_WT


title: TR4PL37_15O

source_name: TR4PL37_15O_Peripheral blood_WT


title: TR4PL37_17G

source_name: TR4PL37_17G_Peripheral blood_WT


title: TR4PL37_17A

source_name: TR4PL37_17A_Peripheral blood_WT


title: TR4PL37_1C

source_name: TR4PL37_1C_Peripheral blood_WT


title: TR4PL37_15I

source_name: TR4PL37_15I_Peripheral blood_WT


title: TR4PL37_15M

source_name: TR4PL37_15M_Peripheral blood_WT


title: TR4PL37_15C

source_name: TR4PL37_15C_Peripheral blood_WT


title: TR4PL37_13O

source_name: TR4PL37_13O_Peripheral blood_WT


title: TR4PL37_15K

source_name: TR4PL37_15K_Peripheral blood_WT


title: TR4PL37_15G

source_name: TR4PL37_15G_Peripheral blood_WT


title: TR4PL37_13M

source_name: TR4PL37_13M_Peripheral blood_WT


title: TR4PL37_17O

source_name: TR4PL37_17O_Peripheral blood_WT


title: TR4PL37_15A

source_name: TR4PL37_15A_Peripheral blood_WT


title: TR4PL37_13K

source_name: TR4PL37_13K_Peripheral blood_WT


title: TR4PL37_13G

source_name: TR4PL37_13G_Peripheral blood_WT


title: TR4PL37_13E

source_name: TR4PL37_13E_Peripheral blood_WT


title: TR4PL37_13A

source_name: TR4PL37_13A_Peripheral blood_WT


title: TR4PL37_11I

source_name: TR4PL37_11I_Peripheral blood_WT


title: TR4PL37_11K

source_name: TR4PL37_11K_Peripheral blood_WT


title: TR4PL37_11G

source_name: TR4PL37_11G_Peripheral blood_WT


title: TR4PL37_11O

source_name: TR4PL37_11O_Peripheral blood_WT


title: TR4PL36_9P


title: TR4PL37_11E

source_name: TR4PL37_11E_Peripheral blood_WT


title: TR4PL37_11A

source_name: TR4PL37_11A_Peripheral blood_WT


title: TR4PL37_11C

source_name: TR4PL37_11C_Peripheral blood_WT


title: TR4PL36_9O


title: TR4PL36_9M


title: TR4PL36_9L


title: TR4PL37_13I

source_name: TR4PL37_13I_Peripheral blood_WT


title: TR4PL36_9I


title: TR4PL36_9K


title: TR4PL36_9E


title: TR4PL37_13C

source_name: TR4PL37_13C_Peripheral blood_WT


title: TR4PL36_9H


title: TR4PL36_9C


title: TR4PL36_9D


title: TR4PL36_7L


title: TR4PL36_7M


title: TR4PL36_7K


title: TR4PL36_7P


title: TR4PL36_7N


title: TR4PL36_7J


title: TR4PL36_7I


title: TR4PL36_7G


title: TR4PL36_9N


title: TR4PL36_7F


title: TR4PL36_7H


title: TR4PL36_9J


title: TR4PL36_9G


title: TR4PL36_7D


title: TR4PL36_7A


title: TR4PL36_5P


title: TR4PL36_5I


title: TR4PL36_7C


title: TR4PL36_5O


title: TR4PL36_5F


title: TR4PL36_7B


title: TR4PL36_5E


title: TR4PL36_9F


title: TR4PL36_5C


title: TR4PL36_5N


title: TR4PL36_5L


title: TR4PL36_5M


title: TR4PL36_5A


title: TR4PL36_3N


title: TR4PL36_3L


title: TR4PL36_5K


title: TR4PL36_3J


title: TR4PL36_5J


title: TR4PL36_5G


title: TR4PL36_3H


title: TR4PL36_7E


title: TR4PL36_3E


title: TR4PL36_5B


title: TR4PL36_3C


title: TR4PL36_5H


title: TR4PL36_3K


title: TR4PL36_3I


title: TR4PL36_3G


title: TR4PL36_3D


title: TR4PL36_23N

source_name: TR4PL36_23N_Peripheral blood_WT


title: TR4PL36_3B


title: TR4PL36_5D


title: TR4PL36_23K

source_name: TR4PL36_23K_Peripheral blood_WT


title: TR4PL36_23M

source_name: TR4PL36_23M_Peripheral blood_WT


title: TR4PL36_23J

source_name: TR4PL36_23J_Peripheral blood_WT


title: TR4PL36_23H

source_name: TR4PL36_23H_Peripheral blood_WT


title: TR4PL36_23I

source_name: TR4PL36_23I_Peripheral blood_WT


title: TR4PL36_23G

source_name: TR4PL36_23G_Peripheral blood_WT


title: TR4PL36_3M


title: TR4PL36_23F

source_name: TR4PL36_23F_Peripheral blood_WT


title: TR4PL36_23E

source_name: TR4PL36_23E_Peripheral blood_WT


title: TR4PL36_23D

source_name: TR4PL36_23D_Peripheral blood_WT


title: TR4PL36_3F


title: TR4PL36_23C

source_name: TR4PL36_23C_Peripheral blood_WT


title: TR4PL36_23A

source_name: TR4PL36_23A_Peripheral blood_WT


title: TR4PL36_23B

source_name: TR4PL36_23B_Peripheral blood_WT


title: TR4PL36_3A


title: TR4PL36_21N

source_name: TR4PL36_21N_Peripheral blood_WT


title: TR4PL36_21M

source_name: TR4PL36_21M_Peripheral blood_WT


title: TR4PL36_21L

source_name: TR4PL36_21L_Peripheral blood_WT


title: TR4PL36_21J

source_name: TR4PL36_21J_Peripheral blood_WT


title: TR4PL36_21D

source_name: TR4PL36_21D_Peripheral blood_WT


title: TR4PL36_21E

source_name: TR4PL36_21E_Peripheral blood_WT


title: TR4PL36_21C

source_name: TR4PL36_21C_Peripheral blood_WT


title: TR4PL36_21G

source_name: TR4PL36_21G_Peripheral blood_WT


title: TR4PL36_21H

source_name: TR4PL36_21H_Peripheral blood_WT


title: TR4PL36_21K

source_name: TR4PL36_21K_Peripheral blood_WT


title: TR4PL36_21I

source_name: TR4PL36_21I_Peripheral blood_WT


title: TR4PL36_21A

source_name: TR4PL36_21A_Peripheral blood_WT


title: TR4PL36_21B

source_name: TR4PL36_21B_Peripheral blood_WT


title: TR4PL36_23L

source_name: TR4PL36_23L_Peripheral blood_WT


title: TR4PL36_1P


title: TR4PL36_1O


title: TR4PL36_1N


title: TR4PL36_1J


title: TR4PL36_1M


title: TR4PL36_1K


title: TR4PL36_21F

source_name: TR4PL36_21F_Peripheral blood_WT


title: TR4PL36_1I


title: TR4PL36_1D


title: TR4PL36_1C


title: TR4PL36_1B


title: TR4PL36_19N

source_name: TR4PL36_19N_Peripheral blood_WT


title: TR4PL36_1A


title: TR4PL36_1F


title: TR4PL36_1G


title: TR4PL36_1L


title: TR4PL36_19M

source_name: TR4PL36_19M_Peripheral blood_WT


title: TR4PL36_1H


title: TR4PL36_19L

source_name: TR4PL36_19L_Peripheral blood_WT


title: TR4PL36_19D

source_name: TR4PL36_19D_Peripheral blood_WT


title: TR4PL36_1E


title: TR4PL36_19B

source_name: TR4PL36_19B_Peripheral blood_WT


title: TR4PL36_19O

source_name: TR4PL36_19O_Peripheral blood_WT


title: TR4PL36_19A

source_name: TR4PL36_19A_Peripheral blood_WT


title: TR4PL36_19J

source_name: TR4PL36_19J_Peripheral blood_WT


title: TR4PL36_19P

source_name: TR4PL36_19P_Peripheral blood_WT


title: TR4PL36_17M


title: TR4PL36_19H

source_name: TR4PL36_19H_Peripheral blood_WT


title: TR4PL36_17N


title: TR4PL36_17K


title: TR4PL36_17I


title: TR4PL36_17J


title: TR4PL36_19E

source_name: TR4PL36_19E_Peripheral blood_WT


title: TR4PL36_17O


title: TR4PL36_19F

source_name: TR4PL36_19F_Peripheral blood_WT


title: TR4PL36_17C


title: TR4PL36_17D


title: TR4PL36_17B


title: TR4PL36_15O


title: TR4PL36_15N


title: TR4PL36_17G


title: TR4PL36_19K

source_name: TR4PL36_19K_Peripheral blood_WT


title: TR4PL36_17H


title: TR4PL36_19C

source_name: TR4PL36_19C_Peripheral blood_WT


title: TR4PL36_17F


title: TR4PL36_15H


title: TR4PL36_17E


title: TR4PL36_15D


title: TR4PL36_15C


title: TR4PL36_15K


title: TR4PL36_13M


title: TR4PL36_17P


title: TR4PL36_17A


title: TR4PL36_15P


title: TR4PL36_15L


title: TR4PL36_15M


title: TR4PL36_13H


title: TR4PL36_15I


title: TR4PL36_15G


title: TR4PL36_15J


title: TR4PL36_13G


title: TR4PL36_13A


title: TR4PL36_15B


title: TR4PL36_13P


title: TR4PL36_11K


title: TR4PL36_13K


title: TR4PL36_11J


title: TR4PL36_11I


title: TR4PL36_13N


title: TR4PL36_13O


title: TR4PL36_13L


title: TR4PL36_11E


title: TR4PL36_11G


title: TR4PL36_11D


title: TR4PL35_9P


title: TR4PL35_9O


title: TR4PL35_9N


title: TR4PL36_13J


title: TR4PL35_9I

source_name: TR4PL35_9I_Peripheral blood_JAK2_HOM


title: TR4PL36_13I


title: TR4PL36_13F


title: TR4PL35_9E


title: TR4PL36_13E


title: TR4PL36_13D


title: TR4PL35_7M


title: TR4PL36_13C


title: TR4PL35_7K


title: TR4PL35_7I


title: TR4PL36_13B


title: TR4PL35_7H


title: TR4PL36_11P


title: TR4PL36_11O


title: TR4PL36_11N


title: TR4PL36_11H


title: TR4PL35_7B


title: TR4PL36_11F


title: TR4PL35_5P


title: TR4PL36_11L


title: TR4PL36_11A


title: TR4PL35_7A


title: TR4PL36_11C


title: TR4PL36_11B


title: TR4PL36_11M


title: TR4PL35_9M


title: TR4PL35_5L


title: TR4PL35_5J


title: TR4PL35_5I


title: TR4PL35_9J


title: TR4PL35_9H


title: TR4PL35_9G


title: TR4PL35_9F


title: TR4PL35_5G


title: TR4PL35_7L


title: TR4PL35_9C


title: TR4PL35_5E


title: TR4PL35_9D


title: TR4PL35_3N


title: TR4PL35_7G


title: TR4PL35_3M


title: TR4PL35_3I


title: TR4PL35_7F


title: TR4PL35_3E


title: TR4PL35_7E


title: TR4PL35_7D


title: TR4PL35_7C


title: TR4PL35_23O

source_name: TR4PL35_23O_Peripheral blood_WT


title: TR4PL35_5N


title: TR4PL35_23N

source_name: TR4PL35_23N_Peripheral blood_WT


title: TR4PL35_5M


title: TR4PL35_5K


title: TR4PL35_5O


title: TR4PL35_23J

source_name: TR4PL35_23J_Peripheral blood_WT


title: TR4PL35_5A


title: TR4PL35_23I

source_name: TR4PL35_23I_Peripheral blood_WT


title: TR4PL35_5F


title: TR4PL35_23E

source_name: TR4PL35_23E_Peripheral blood_WT


title: TR4PL35_23F

source_name: TR4PL35_23F_Peripheral blood_WT


title: TR4PL35_5H


title: TR4PL35_3J


title: TR4PL35_3H


title: TR4PL35_3F


title: TR4PL35_23G

source_name: TR4PL35_23G_Peripheral blood_WT


title: TR4PL35_5C


title: TR4PL35_3B


title: TR4PL35_3C


title: TR4PL35_3A


title: TR4PL35_5D


title: TR4PL35_23P

source_name: TR4PL35_23P_Peripheral blood_WT


title: TR4PL35_21I

source_name: TR4PL35_21I_Peripheral blood_WT


title: TR4PL35_23L

source_name: TR4PL35_23L_Peripheral blood_WT


title: TR4PL35_21H

source_name: TR4PL35_21H_Peripheral blood_WT


title: TR4PL35_21D

source_name: TR4PL35_21D_Peripheral blood_WT


title: TR4PL35_21B

source_name: TR4PL35_21B_Peripheral blood_WT


title: TR4PL35_23K

source_name: TR4PL35_23K_Peripheral blood_WT


title: TR4PL35_21G

source_name: TR4PL35_21G_Peripheral blood_WT


title: TR4PL35_3G


title: TR4PL35_23H

source_name: TR4PL35_23H_Peripheral blood_WT


title: TR4PL35_21A

source_name: TR4PL35_21A_Peripheral blood_WT


title: TR4PL35_1N


title: TR4PL35_23C

source_name: TR4PL35_23C_Peripheral blood_WT


title: TR4PL35_23A

source_name: TR4PL35_23A_Peripheral blood_WT


title: TR4PL35_23B

source_name: TR4PL35_23B_Peripheral blood_WT


title: TR4PL35_23M

source_name: TR4PL35_23M_Peripheral blood_WT


title: TR4PL35_21O

source_name: TR4PL35_21O_Peripheral blood_WT


title: TR4PL35_1K


title: TR4PL35_21K

source_name: TR4PL35_21K_Peripheral blood_WT


title: TR4PL35_21N

source_name: TR4PL35_21N_Peripheral blood_WT


title: TR4PL35_1G


title: TR4PL35_21J

source_name: TR4PL35_21J_Peripheral blood_WT


title: TR4PL35_1C


title: TR4PL35_21L

source_name: TR4PL35_21L_Peripheral blood_WT


title: TR4PL35_21C

source_name: TR4PL35_21C_Peripheral blood_WT


title: TR4PL35_21E

source_name: TR4PL35_21E_Peripheral blood_WT


title: TR4PL35_23D

source_name: TR4PL35_23D_Peripheral blood_WT


title: TR4PL35_1P


title: TR4PL35_1O


title: TR4PL35_21P

source_name: TR4PL35_21P_Peripheral blood_WT


title: TR4PL35_19L

source_name: TR4PL35_19L_Peripheral blood_WT


title: TR4PL35_1H


title: TR4PL35_19I

source_name: TR4PL35_19I_Peripheral blood_WT


title: TR4PL35_1L


title: TR4PL35_1M


title: TR4PL35_1J


title: TR4PL35_1I


title: TR4PL35_19C

source_name: TR4PL35_19C_Peripheral blood_WT


title: TR4PL35_1F


title: TR4PL35_17O


title: TR4PL35_17N


title: TR4PL35_17M


title: TR4PL35_17K


title: TR4PL35_1A


title: TR4PL35_1E


title: TR4PL35_19M

source_name: TR4PL35_19M_Peripheral blood_WT


title: TR4PL35_1D


title: TR4PL35_19J

source_name: TR4PL35_19J_Peripheral blood_WT


title: TR4PL35_19H

source_name: TR4PL35_19H_Peripheral blood_WT


title: TR4PL35_19G

source_name: TR4PL35_19G_Peripheral blood_WT


title: TR4PL35_1B


title: TR4PL35_19F

source_name: TR4PL35_19F_Peripheral blood_WT


title: TR4PL35_19N

source_name: TR4PL35_19N_Peripheral blood_WT


title: TR4PL35_19D

source_name: TR4PL35_19D_Peripheral blood_WT


title: TR4PL35_19E

source_name: TR4PL35_19E_Peripheral blood_WT


title: TR4PL35_19B

source_name: TR4PL35_19B_Peripheral blood_WT


title: TR4PL35_17A


title: TR4PL35_17L


title: TR4PL35_17J


title: TR4PL35_17G


title: TR4PL35_17I


title: TR4PL35_19O

source_name: TR4PL35_19O_Peripheral blood_WT


title: TR4PL35_17F


title: TR4PL35_17E


title: TR4PL35_15L


title: TR4PL35_15K


title: TR4PL35_15M


title: TR4PL35_17C


title: TR4PL35_17B


title: TR4PL35_17H


title: TR4PL35_15C


title: TR4PL35_15N


title: TR4PL35_15D


title: TR4PL35_15G


title: TR4PL35_15A

source_name: TR4PL35_15A_Peripheral blood_JAK2_HOM


title: TR4PL35_13N


title: TR4PL35_13P


title: TR4PL35_15I


title: TR4PL35_13M


title: TR4PL35_13K


title: TR4PL35_13L


title: TR4PL35_15O


title: TR4PL35_15P


title: TR4PL35_13I


title: TR4PL35_13G


title: TR4PL35_13H


title: TR4PL35_13C


title: TR4PL35_13F


title: TR4PL35_13D


title: TR4PL35_15H


title: TR4PL35_15J


title: TR4PL35_13A


title: TR4PL35_13E


title: TR4PL35_11P


title: TR4PL35_11I


title: TR4PL35_11H

source_name: TR4PL35_11H_Peripheral blood_JAK2_HOM


title: TR4PL35_11N


title: TR4PL35_11O


title: TR4PL35_11K


title: TR4PL35_11G


title: TR4PL35_11J


title: TR4PL35_11F


title: TR4PL35_11D


title: TR4PL35_11E


title: TR4PL35_11A


title: TR4PL35_11C


title: TR4PL35_11B


title: TR4PL34_9P

source_name: TR4PL34_9P_Peripheral blood_WT


title: TR4PL35_13J


title: TR4PL35_13B


title: TR4PL34_9N

source_name: TR4PL34_9N_Peripheral blood_WT


title: TR4PL34_9J

source_name: TR4PL34_9J_Peripheral blood_WT


title: TR4PL34_9O

source_name: TR4PL34_9O_Peripheral blood_WT


title: TR4PL34_9I

source_name: TR4PL34_9I_Peripheral blood_WT


title: TR4PL34_9H

source_name: TR4PL34_9H_Peripheral blood_WT


title: TR4PL34_9F

source_name: TR4PL34_9F_Peripheral blood_WT


title: TR4PL34_9G

source_name: TR4PL34_9G_Peripheral blood_WT


title: TR4PL34_9E

source_name: TR4PL34_9E_Peripheral blood_WT


title: TR4PL34_9D

source_name: TR4PL34_9D_Peripheral blood_WT


title: TR4PL34_9C

source_name: TR4PL34_9C_Peripheral blood_WT


title: TR4PL34_8A

source_name: TR4PL34_8A_Peripheral blood_WT


title: TR4PL34_8B

source_name: TR4PL34_8B_Peripheral blood_WT


title: TR4PL34_8H

source_name: TR4PL34_8H_Peripheral blood_WT


title: TR4PL34_8G

source_name: TR4PL34_8G_Peripheral blood_WT


title: TR4PL34_7L

source_name: TR4PL34_7L_Peripheral blood_WT


title: TR4PL34_8N

source_name: TR4PL34_8N_Peripheral blood_WT


title: TR4PL34_7J

source_name: TR4PL34_7J_Peripheral blood_WT


title: TR4PL35_11L


title: TR4PL34_8D

source_name: TR4PL34_8D_Peripheral blood_WT


title: TR4PL34_8F

source_name: TR4PL34_8F_Peripheral blood_WT


title: TR4PL34_9M

source_name: TR4PL34_9M_Peripheral blood_WT


title: TR4PL34_7H

source_name: TR4PL34_7H_Peripheral blood_WT


title: TR4PL34_7I

source_name: TR4PL34_7I_Peripheral blood_WT


title: TR4PL34_7G

source_name: TR4PL34_7G_Peripheral blood_WT


title: TR4PL34_8C

source_name: TR4PL34_8C_Peripheral blood_WT


title: TR4PL34_8K

source_name: TR4PL34_8K_Peripheral blood_WT


title: TR4PL34_7C

source_name: TR4PL34_7C_Peripheral blood_WT


title: TR4PL34_8J

source_name: TR4PL34_8J_Peripheral blood_WT


title: TR4PL34_7E

source_name: TR4PL34_7E_Peripheral blood_WT


title: TR4PL34_8E

source_name: TR4PL34_8E_Peripheral blood_WT


title: TR4PL34_7F

source_name: TR4PL34_7F_Peripheral blood_WT


title: TR4PL34_7D

source_name: TR4PL34_7D_Peripheral blood_WT


title: TR4PL34_8M

source_name: TR4PL34_8M_Peripheral blood_WT


title: TR4PL34_7A

source_name: TR4PL34_7A_Peripheral blood_WT


title: TR4PL34_6P

source_name: TR4PL34_6P_Peripheral blood_WT


title: TR4PL34_7M

source_name: TR4PL34_7M_Peripheral blood_WT


title: TR4PL34_6O

source_name: TR4PL34_6O_Peripheral blood_WT


title: TR4PL34_7B

source_name: TR4PL34_7B_Peripheral blood_WT


title: TR4PL34_7N

source_name: TR4PL34_7N_Peripheral blood_WT


title: TR4PL34_6L

source_name: TR4PL34_6L_Peripheral blood_WT


title: TR4PL34_6M

source_name: TR4PL34_6M_Peripheral blood_WT


title: TR4PL34_6K

source_name: TR4PL34_6K_Peripheral blood_WT


title: TR4PL34_6J

source_name: TR4PL34_6J_Peripheral blood_WT


title: TR4PL34_7K

source_name: TR4PL34_7K_Peripheral blood_WT


title: TR4PL34_6N

source_name: TR4PL34_6N_Peripheral blood_WT


title: TR4PL34_6F

source_name: TR4PL34_6F_Peripheral blood_WT


title: TR4PL34_6I

source_name: TR4PL34_6I_Peripheral blood_WT


title: TR4PL34_6G

source_name: TR4PL34_6G_Peripheral blood_WT


title: TR4PL34_6H

source_name: TR4PL34_6H_Peripheral blood_WT


title: TR4PL34_6E

source_name: TR4PL34_6E_Peripheral blood_WT


title: TR4PL34_6D

source_name: TR4PL34_6D_Peripheral blood_WT


title: TR4PL34_6C

source_name: TR4PL34_6C_Peripheral blood_WT


title: TR4PL34_6A

source_name: TR4PL34_6A_Peripheral blood_WT


title: TR4PL34_5P

source_name: TR4PL34_5P_Peripheral blood_WT


title: TR4PL34_6B

source_name: TR4PL34_6B_Peripheral blood_WT


title: TR4PL34_5O

source_name: TR4PL34_5O_Peripheral blood_WT


title: TR4PL34_5N

source_name: TR4PL34_5N_Peripheral blood_WT


title: TR4PL34_5I

source_name: TR4PL34_5I_Peripheral blood_WT


title: TR4PL34_5M

source_name: TR4PL34_5M_Peripheral blood_WT


title: TR4PL34_5J

source_name: TR4PL34_5J_Peripheral blood_WT


title: TR4PL34_5F

source_name: TR4PL34_5F_Peripheral blood_WT


title: TR4PL34_5L

source_name: TR4PL34_5L_Peripheral blood_WT


title: TR4PL34_5E

source_name: TR4PL34_5E_Peripheral blood_WT


title: TR4PL34_5H

source_name: TR4PL34_5H_Peripheral blood_WT


title: TR4PL34_4N

source_name: TR4PL34_4N_Peripheral blood_WT


title: TR4PL34_5A

source_name: TR4PL34_5A_Peripheral blood_WT


title: TR4PL34_5D

source_name: TR4PL34_5D_Peripheral blood_WT


title: TR4PL34_5G

source_name: TR4PL34_5G_Peripheral blood_WT


title: TR4PL34_5B

source_name: TR4PL34_5B_Peripheral blood_WT


title: TR4PL34_4M

source_name: TR4PL34_4M_Peripheral blood_WT


title: TR4PL34_4L

source_name: TR4PL34_4L_Peripheral blood_WT


title: TR4PL34_4J

source_name: TR4PL34_4J_Peripheral blood_WT


title: TR4PL34_4D

source_name: TR4PL34_4D_Peripheral blood_WT


title: TR4PL34_4A

source_name: TR4PL34_4A_Peripheral blood_WT


title: TR4PL34_3H

source_name: TR4PL34_3H_Peripheral blood_WT


title: TR4PL34_3P

source_name: TR4PL34_3P_Peripheral blood_WT


title: TR4PL34_3B

source_name: TR4PL34_3B_Peripheral blood_WT


title: TR4PL34_4I

source_name: TR4PL34_4I_Peripheral blood_WT


title: TR4PL34_3F

source_name: TR4PL34_3F_Peripheral blood_WT


title: TR4PL34_4H

source_name: TR4PL34_4H_Peripheral blood_WT


title: TR4PL34_3G

source_name: TR4PL34_3G_Peripheral blood_WT


title: TR4PL34_3E

source_name: TR4PL34_3E_Peripheral blood_WT


title: TR4PL34_4C

source_name: TR4PL34_4C_Peripheral blood_WT


title: TR4PL34_3C

source_name: TR4PL34_3C_Peripheral blood_WT


title: TR4PL34_3J

source_name: TR4PL34_3J_Peripheral blood_WT


title: TR4PL34_4F

source_name: TR4PL34_4F_Peripheral blood_WT


title: TR4PL34_3K

source_name: TR4PL34_3K_Peripheral blood_WT


title: TR4PL34_3D

source_name: TR4PL34_3D_Peripheral blood_WT


title: TR4PL34_2P

source_name: TR4PL34_2P_Peripheral blood_WT


title: TR4PL34_3A

source_name: TR4PL34_3A_Peripheral blood_WT


title: TR4PL34_2N

source_name: TR4PL34_2N_Peripheral blood_WT


title: TR4PL34_2M

source_name: TR4PL34_2M_Peripheral blood_WT


title: TR4PL34_3N

source_name: TR4PL34_3N_Peripheral blood_WT


title: TR4PL34_4B

source_name: TR4PL34_4B_Peripheral blood_WT


title: TR4PL34_2I

source_name: TR4PL34_2I_Peripheral blood_WT


title: TR4PL34_3I

source_name: TR4PL34_3I_Peripheral blood_WT


title: TR4PL34_4G

source_name: TR4PL34_4G_Peripheral blood_WT


title: TR4PL34_3M

source_name: TR4PL34_3M_Peripheral blood_WT


title: TR4PL34_2J

source_name: TR4PL34_2J_Peripheral blood_WT


title: TR4PL34_2L

source_name: TR4PL34_2L_Peripheral blood_WT


title: TR4PL34_2K

source_name: TR4PL34_2K_Peripheral blood_WT


title: TR4PL34_2F

source_name: TR4PL34_2F_Peripheral blood_WT


title: TR4PL34_2E

source_name: TR4PL34_2E_Peripheral blood_WT


title: TR4PL34_2C

source_name: TR4PL34_2C_Peripheral blood_WT


title: TR4PL34_2B

source_name: TR4PL34_2B_Peripheral blood_WT


title: TR4PL34_2D

source_name: TR4PL34_2D_Peripheral blood_WT


title: TR4PL34_2A

source_name: TR4PL34_2A_Peripheral blood_WT


title: TR4PL34_22G

source_name: TR4PL34_22G_Peripheral blood_JAK2_HET


title: TR4PL34_22F

source_name: TR4PL34_22F_Peripheral blood_JAK2_HET


title: TR4PL34_22I

source_name: TR4PL34_22I_Peripheral blood_JAK2_HET


title: TR4PL34_22B

source_name: TR4PL34_22B_Peripheral blood_JAK2_HET


title: TR4PL34_22J

source_name: TR4PL34_22J_Peripheral blood_JAK2_HET


title: TR4PL34_22D

source_name: TR4PL34_22D_Peripheral blood_JAK2_WT


title: TR4PL34_22E

source_name: TR4PL34_22E_Peripheral blood_JAK2_WT


title: TR4PL34_22L

source_name: TR4PL34_22L_Peripheral blood_JAK2_WT


title: TR4PL34_22C

source_name: TR4PL34_22C_Peripheral blood_JAK2_HOM


title: TR4PL34_20O

source_name: TR4PL34_20O_Peripheral blood_JAK2_HET


title: TR4PL34_22M

source_name: TR4PL34_22M_Peripheral blood_JAK2_HET


title: TR4PL34_22N

source_name: TR4PL34_22N_Peripheral blood_JAK2_HET


title: TR4PL34_2H

source_name: TR4PL34_2H_Peripheral blood_WT


title: TR4PL34_20P

source_name: TR4PL34_20P_Peripheral blood_JAK2_WT


title: TR4PL34_3L

source_name: TR4PL34_3L_Peripheral blood_WT


title: TR4PL34_20N

source_name: TR4PL34_20N_Peripheral blood_JAK2_WT


title: TR4PL34_22H

source_name: TR4PL34_22H_Peripheral blood_JAK2_HET


title: TR4PL34_20J

source_name: TR4PL34_20J_Peripheral blood_JAK2_HET


title: TR4PL34_22K

source_name: TR4PL34_22K_Peripheral blood_JAK2_HET


title: TR4PL34_20L

source_name: TR4PL34_20L_Peripheral blood_JAK2_HET


title: TR4PL34_20M

source_name: TR4PL34_20M_Peripheral blood_JAK2_WT


title: TR4PL34_20G

source_name: TR4PL34_20G_Peripheral blood_JAK2_HET


title: TR4PL34_20H

source_name: TR4PL34_20H_Peripheral blood_JAK2_HOM


title: TR4PL34_20F

source_name: TR4PL34_20F_Peripheral blood_JAK2_WT


title: TR4PL34_20E

source_name: TR4PL34_20E_Peripheral blood_JAK2_HET


title: TR4PL34_20D

source_name: TR4PL34_20D_Peripheral blood_JAK2_HET


title: TR4PL34_20C

source_name: TR4PL34_20C_Peripheral blood_JAK2_WT


title: TR4PL34_20B

source_name: TR4PL34_20B_Peripheral blood_JAK2_WT


title: TR4PL34_1P

source_name: TR4PL34_1P_Peripheral blood_WT


title: TR4PL34_20A

source_name: TR4PL34_20A_Peripheral blood_JAK2_HET


title: TR4PL34_1L

source_name: TR4PL34_1L_Peripheral blood_WT


title: TR4PL34_1G

source_name: TR4PL34_1G_Peripheral blood_WT


title: TR4PL34_1N

source_name: TR4PL34_1N_Peripheral blood_WT


title: TR4PL34_1O

source_name: TR4PL34_1O_Peripheral blood_WT


title: TR4PL34_1M

source_name: TR4PL34_1M_Peripheral blood_WT


title: TR4PL34_1F

source_name: TR4PL34_1F_Peripheral blood_WT


title: TR4PL34_1J

source_name: TR4PL34_1J_Peripheral blood_WT


title: TR4PL34_1K

source_name: TR4PL34_1K_Peripheral blood_WT


title: TR4PL34_1B

source_name: TR4PL34_1B_Peripheral blood_WT


title: TR4PL34_1D

source_name: TR4PL34_1D_Peripheral blood_WT


title: TR4PL34_1H

source_name: TR4PL34_1H_Peripheral blood_WT


title: TR4PL34_1I

source_name: TR4PL34_1I_Peripheral blood_WT


title: TR4PL34_1A

source_name: TR4PL34_1A_Peripheral blood_WT


title: TR4PL34_18L

source_name: TR4PL34_18L_Peripheral blood_JAK2_WT


title: TR4PL34_1C

source_name: TR4PL34_1C_Peripheral blood_WT


title: TR4PL34_1E

source_name: TR4PL34_1E_Peripheral blood_WT


title: TR4PL34_18J

source_name: TR4PL34_18J_Peripheral blood_JAK2_HET


title: TR4PL34_18I

source_name: TR4PL34_18I_Peripheral blood_JAK2_WT


title: TR4PL34_18H

source_name: TR4PL34_18H_Peripheral blood_JAK2_WT


title: TR4PL34_18G

source_name: TR4PL34_18G_Peripheral blood_JAK2_WT


title: TR4PL34_18E

source_name: TR4PL34_18E_Peripheral blood_JAK2_HET


title: TR4PL34_18F

source_name: TR4PL34_18F_Peripheral blood_JAK2_HET


title: TR4PL34_18A

source_name: TR4PL34_18A_Peripheral blood_JAK2_HET


title: TR4PL34_18D

source_name: TR4PL34_18D_Peripheral blood_JAK2_HET


title: TR4PL34_18B

source_name: TR4PL34_18B_Peripheral blood_JAK2_HET


title: TR4PL34_17P

source_name: TR4PL34_17P_Peripheral blood_JAK2_WT


title: TR4PL34_18N

source_name: TR4PL34_18N_Peripheral blood_JAK2_HET


title: TR4PL34_18M

source_name: TR4PL34_18M_Peripheral blood_JAK2_WT


title: TR4PL34_17O

source_name: TR4PL34_17O_Peripheral blood_JAK2_HET


title: TR4PL34_18K

source_name: TR4PL34_18K_Peripheral blood_JAK2_HET


title: TR4PL34_17M

source_name: TR4PL34_17M_Peripheral blood_JAK2_HET


title: TR4PL34_17J

source_name: TR4PL34_17J_Peripheral blood_JAK2_HET


title: TR4PL34_17F

source_name: TR4PL34_17F_Peripheral blood_JAK2_HOM


title: TR4PL34_16O

source_name: TR4PL34_16O_Peripheral blood_JAK2_WT


title: TR4PL34_17H

source_name: TR4PL34_17H_Peripheral blood_JAK2_HET


title: TR4PL34_17I

source_name: TR4PL34_17I_Peripheral blood_JAK2_HET


title: TR4PL34_17K

source_name: TR4PL34_17K_Peripheral blood_JAK2_WT


title: TR4PL34_17L

source_name: TR4PL34_17L_Peripheral blood_JAK2_WT


title: TR4PL34_17G

source_name: TR4PL34_17G_Peripheral blood_JAK2_HET


title: TR4PL34_16M

source_name: TR4PL34_16M_Peripheral blood_JAK2_WT


title: TR4PL34_16K

source_name: TR4PL34_16K_Peripheral blood_JAK2_HET


title: TR4PL34_16J

source_name: TR4PL34_16J_Peripheral blood_JAK2_HET


title: TR4PL34_16L

source_name: TR4PL34_16L_Peripheral blood_JAK2_HET


title: TR4PL34_16P

source_name: TR4PL34_16P_Peripheral blood_JAK2_WT


title: TR4PL34_16N

source_name: TR4PL34_16N_Peripheral blood_JAK2_HET


title: TR4PL34_16H

source_name: TR4PL34_16H_Peripheral blood_JAK2_WT


title: TR4PL34_16I

source_name: TR4PL34_16I_Peripheral blood_JAK2_HET


title: TR4PL34_16A

source_name: TR4PL34_16A_Peripheral blood_JAK2_HET


title: TR4PL34_16D

source_name: TR4PL34_16D_Peripheral blood_JAK2_HET


title: TR4PL34_15P

source_name: TR4PL34_15P_Peripheral blood_JAK2_HET


title: TR4PL34_15N

source_name: TR4PL34_15N_Peripheral blood_JAK2_HET


title: TR4PL34_16B

source_name: TR4PL34_16B_Peripheral blood_JAK2_HET


title: TR4PL34_15M

source_name: TR4PL34_15M_Peripheral blood_JAK2_HET


title: TR4PL34_15O

source_name: TR4PL34_15O_Peripheral blood_JAK2_HET


title: TR4PL34_15K

source_name: TR4PL34_15K_Peripheral blood_JAK2_WT


title: TR4PL34_15I

source_name: TR4PL34_15I_Peripheral blood_JAK2_HET


title: TR4PL34_15J

source_name: TR4PL34_15J_Peripheral blood_JAK2_HET


title: TR4PL34_15H

source_name: TR4PL34_15H_Peripheral blood_JAK2_WT


title: TR4PL34_15G

source_name: TR4PL34_15G_Peripheral blood_JAK2_HET


title: TR4PL34_15C

source_name: TR4PL34_15C_Peripheral blood_JAK2_HET


title: TR4PL34_14P

source_name: TR4PL34_14P_Peripheral blood_JAK2_HET


title: TR4PL34_15B

source_name: TR4PL34_15B_Peripheral blood_JAK2_WT


title: TR4PL34_14N

source_name: TR4PL34_14N_Peripheral blood_JAK2_HET


title: TR4PL34_14J

source_name: TR4PL34_14J_Peripheral blood_JAK2_HET


title: TR4PL34_15A

source_name: TR4PL34_15A_Peripheral blood_JAK2_WT


title: TR4PL34_14K

source_name: TR4PL34_14K_Peripheral blood_JAK2_HET


title: TR4PL34_14G

source_name: TR4PL34_14G_Peripheral blood_JAK2_HET


title: TR4PL34_15D

source_name: TR4PL34_15D_Peripheral blood_JAK2_WT


title: TR4PL34_14L

source_name: TR4PL34_14L_Peripheral blood_JAK2_HET


title: TR4PL34_14F

source_name: TR4PL34_14F_Peripheral blood_JAK2_HET


title: TR4PL34_14H

source_name: TR4PL34_14H_Peripheral blood_JAK2_HET


title: TR4PL34_14O

source_name: TR4PL34_14O_Peripheral blood_JAK2_HET


title: TR4PL34_14M

source_name: TR4PL34_14M_Peripheral blood_JAK2_HET


title: TR4PL34_14D

source_name: TR4PL34_14D_Peripheral blood_JAK2_HET


title: TR4PL34_14E

source_name: TR4PL34_14E_Peripheral blood_JAK2_WT


title: TR4PL34_14I

source_name: TR4PL34_14I_Peripheral blood_JAK2_HET


title: TR4PL34_13O

source_name: TR4PL34_13O_Peripheral blood_JAK2_WT


title: TR4PL34_15L

source_name: TR4PL34_15L_Peripheral blood_JAK2_HET


title: TR4PL34_13N

source_name: TR4PL34_13N_Peripheral blood_JAK2_WT


title: TR4PL34_13M

source_name: TR4PL34_13M_Peripheral blood_JAK2_HET


title: TR4PL34_13K

source_name: TR4PL34_13K_Peripheral blood_JAK2_WT


title: TR4PL34_13L

source_name: TR4PL34_13L_Peripheral blood_JAK2_WT


title: TR4PL34_13I

source_name: TR4PL34_13I_Peripheral blood_JAK2_WT


title: TR4PL34_13P

source_name: TR4PL34_13P_Peripheral blood_JAK2_HET


title: TR4PL34_13G

source_name: TR4PL34_13G_Peripheral blood_JAK2_HET


title: TR4PL34_13H

source_name: TR4PL34_13H_Peripheral blood_JAK2_WT


title: TR4PL34_13J

source_name: TR4PL34_13J_Peripheral blood_JAK2_WT


title: TR4PL34_13D

source_name: TR4PL34_13D_Peripheral blood_JAK2_HET


title: TR4PL34_13F

source_name: TR4PL34_13F_Peripheral blood_JAK2_HET


title: TR4PL34_13C

source_name: TR4PL34_13C_Peripheral blood_JAK2_WT


title: TR4PL34_12P

source_name: TR4PL34_12P_Peripheral blood_JAK2_HET


title: TR4PL34_12K

source_name: TR4PL34_12K_Peripheral blood_JAK2_HET


title: TR4PL34_14B

source_name: TR4PL34_14B_Peripheral blood_JAK2_WT


title: TR4PL34_12I

source_name: TR4PL34_12I_Peripheral blood_JAK2_HET


title: TR4PL34_12H

source_name: TR4PL34_12H_Peripheral blood_JAK2_HET


title: TR4PL34_12J

source_name: TR4PL34_12J_Peripheral blood_JAK2_HET


title: TR4PL34_13A

source_name: TR4PL34_13A_Peripheral blood_JAK2_HET


title: TR4PL34_12F

source_name: TR4PL34_12F_Peripheral blood_JAK2_WT


title: TR4PL34_12D

source_name: TR4PL34_12D_Peripheral blood_JAK2_HET


title: TR4PL34_14A

source_name: TR4PL34_14A_Peripheral blood_JAK2_HET


title: TR4PL34_12B

source_name: TR4PL34_12B_Peripheral blood_JAK2_HET


title: TR4PL34_12E

source_name: TR4PL34_12E_Peripheral blood_JAK2_WT


title: TR4PL34_12C

source_name: TR4PL34_12C_Peripheral blood_JAK2_HET


title: TR4PL34_11P

source_name: TR4PL34_11P_Peripheral blood_JAK2_HET


title: TR4PL34_11O

source_name: TR4PL34_11O_Peripheral blood_JAK2_HET


title: TR4PL34_11L

source_name: TR4PL34_11L_Peripheral blood_JAK2_HET


title: TR4PL34_11N

source_name: TR4PL34_11N_Peripheral blood_JAK2_HET


title: TR4PL34_11H

source_name: TR4PL34_11H_Peripheral blood_JAK2_HET


title: TR4PL34_11I

source_name: TR4PL34_11I_Peripheral blood_JAK2_HET


title: TR4PL34_11J

source_name: TR4PL34_11J_Peripheral blood_JAK2_WT


title: TR4PL34_13B

source_name: TR4PL34_13B_Peripheral blood_JAK2_HET


title: TR4PL34_11E

source_name: TR4PL34_11E_Peripheral blood_JAK2_HET


title: TR4PL34_11B

source_name: TR4PL34_11B_Peripheral blood_JAK2_WT


title: TR4PL34_12L

source_name: TR4PL34_12L_Peripheral blood_JAK2_WT


title: TR4PL34_11D

source_name: TR4PL34_11D_Peripheral blood_JAK2_WT


title: TR4PL34_11F

source_name: TR4PL34_11F_Peripheral blood_JAK2_WT


title: TR4PL34_11A

source_name: TR4PL34_11A_Peripheral blood_JAK2_HET


title: TR4PL34_12G

source_name: TR4PL34_12G_Peripheral blood_JAK2_HET


title: TR4PL34_10P

source_name: TR4PL34_10P_Peripheral blood_JAK2_WT


title: TR4PL34_10M

source_name: TR4PL34_10M_Peripheral blood_JAK2_HET


title: TR4PL34_10H

source_name: TR4PL34_10H_Peripheral blood_JAK2_HET


title: TR4PL34_10O

source_name: TR4PL34_10O_Peripheral blood_JAK2_WT


title: TR4PL34_10N

source_name: TR4PL34_10N_Peripheral blood_JAK2_HET


title: TR4PL34_11C

source_name: TR4PL34_11C_Peripheral blood_JAK2_WT


title: TR4PL34_10I

source_name: TR4PL34_10I_Peripheral blood_JAK2_HET


title: TR4PL34_10G

source_name: TR4PL34_10G_Peripheral blood_JAK2_HET


title: TR4PL34_10D

source_name: TR4PL34_10D_Peripheral blood_JAK2_HET


title: TR4PL34_10J

source_name: TR4PL34_10J_Peripheral blood_JAK2_WT


title: TR4PL32_9O


title: TR4PL32_9N


title: TR4PL34_10F

source_name: TR4PL34_10F_Peripheral blood_JAK2_WT


title: TR4PL34_10C

source_name: TR4PL34_10C_Peripheral blood_JAK2_WT


title: TR4PL32_9M


title: TR4PL32_9H


title: TR4PL32_9I


title: TR4PL32_9G


title: TR4PL32_9D


title: TR4PL32_9E


title: TR4PL32_9C


title: TR4PL32_8L


title: TR4PL32_8N


title: TR4PL32_8J


title: TR4PL32_8I


title: TR4PL32_9P


title: TR4PL32_9K


title: TR4PL32_8E


title: TR4PL32_9J


title: TR4PL32_8C


title: TR4PL32_8F


title: TR4PL32_7J


title: TR4PL32_8G


title: TR4PL32_7M


title: TR4PL32_7N


title: TR4PL32_7K


title: TR4PL32_7L


title: TR4PL32_7I


title: TR4PL32_7G


title: TR4PL32_7C


title: TR4PL32_7F


title: TR4PL32_7D


title: TR4PL32_8K


title: TR4PL32_8H


title: TR4PL32_6P

source_name: TR4PL32_6P_Peripheral blood_WT


title: TR4PL32_8A


title: TR4PL32_6O

source_name: TR4PL32_6O_Peripheral blood_WT


title: TR4PL32_6N

source_name: TR4PL32_6N_Peripheral blood_WT


title: TR4PL32_8D


title: TR4PL32_6K

source_name: TR4PL32_6K_Peripheral blood_WT


title: TR4PL32_6J

source_name: TR4PL32_6J_Peripheral blood_WT


title: TR4PL32_7H


title: TR4PL32_6I

source_name: TR4PL32_6I_Peripheral blood_WT


title: TR4PL32_6G

source_name: TR4PL32_6G_Peripheral blood_WT


title: TR4PL32_7B


title: TR4PL32_6F

source_name: TR4PL32_6F_Peripheral blood_WT


title: TR4PL32_6C

source_name: TR4PL32_6C_Peripheral blood_WT


title: TR4PL32_6H

source_name: TR4PL32_6H_Peripheral blood_WT


title: TR4PL32_5P

source_name: TR4PL32_5P_Peripheral blood_WT


title: TR4PL32_5O

source_name: TR4PL32_5O_Peripheral blood_WT


title: TR4PL32_6D

source_name: TR4PL32_6D_Peripheral blood_WT


title: TR4PL32_5M

source_name: TR4PL32_5M_Peripheral blood_WT


title: TR4PL32_6A

source_name: TR4PL32_6A_Peripheral blood_WT


title: TR4PL32_6B

source_name: TR4PL32_6B_Peripheral blood_WT


title: TR4PL32_6M

source_name: TR4PL32_6M_Peripheral blood_WT


title: TR4PL32_5L

source_name: TR4PL32_5L_Peripheral blood_WT