title: CHEC_free_mnase_30sec

city: GENEVA

first: Benjamin

last: Albert

address: 30, quai Ernest-Ansermet

department: Bimol

institute: University of Genevea

data_processing:
Library strategy: ChEC-seq
For ChIP-Seq raw reads were aligned to the sacCer3 and 972-h pombe genome assembly using Bowtie2 with options "-k 20 --end-to-end --sensitive -X 800".
For ChIP-Seq, data were filtered to retain only the mapped reads with at most 5 hits in the reference.
For ChIP-Seq, reads that mapped to both sacCer3 and 972-h genomes were discarded.
For ChIP-Seq, the reads were shifted by 100 bp and extended by 50 bp
For ChIP-Seq, reads mapped uniquely to the sacCer3 genome were first normalized to total number of mapped reads in sacCer3 and then to total number of uniquely mapped reads in 972-h pombe in the same sample as described in (Hu et al. 2015)
For ChEC-Seq, Pairs of FASTQ files were mapped to the sacCer3 genome assembly using Novoalign (Novocraft), generating SAM files that were then converted to tag directories with HOMER (PMID 20513432) (homer.ucsd.edu) for further analysis.
Genome_build: sacCer3, 972-h pombe
Supplementary_files_format_and_content: wig and bigwig files were obtained using HTSStation (http://htsstation.epfl.ch)
Supplementary_files_format_and_content: ChEC-seq data were visualized as reads per million (RPM)-normalized single-base resolution bedgraph tracks.

organism: Saccharomyces cerevisiae

source_name: whole organism

growth_protocol: cells were grown at 30 degrees in YPAD to mid-log phase before treatment

extract_protocol:
TAP and RNAPII ChIP-seq was performed as described previously (Ribaud et al., 2012) (PMID: 21952045). Chromatin for MNase digestion was prepared essentially as described in Kubik et al., 2015 (GSE73337) (PMID: 26545077).Cells were pelleted at 3,000 x g and washed three times with 1 mL Buffer A (15 mM Tris pH 7.5, 80 mM KCl, 0.1 mM EGTA, 0.2 mM spermine, 0.5 mM spermidine, Roche cOmplete EDTA-free mini protease inhibitors, 1 mM PMSF), centrifuging as above between washes. Cells were resuspended in 600 µL Buffer A containing 0.1% digitonin and permeabilized at 30C for 5 min. CaCl2 was added to 2 mM and ChEC digests were performed at 30C. 5 min after CaCl2 addition, a 100 µL aliquot of the digest was transferred to a tube containing 100 µL 2X stop buffer (400 mM NaCl, 20 mM EDTA, 4 mM EGTA) and 1% SDS. Protein was then was digested with 80 µg proteinase K at 55C for 30 m. Nucleic acids were extracted with an equal volume of phenol/chloroform/isoamyl alcohol (25:24:1) and precipitated with 2.5 volumes 100% ethanol and 30 µg Glycogen (Roche). Pellets were washed once with 1 mL 100% ethanol, dried, and resuspended in 30 µL 0.1X TE buffer, pH 8.0. RNA was digested with 10 µg RNase A at 37C for 20 m. Prior to generating sequencing libraries, ChEC DNA was subjected to size-selection using Ampure XP beads (ThermoFischer). A beads:sample ratio of 2.5:1 was used and the unbound fraction, containing small DNA fragments, was extracted to remove RNase and precipitated as above, and used for library preparation.
Libraries were prepared essentially as described in Henikoff et al., 2011 (GSE30551) (PMID: 22025710

value: leu2-3,112 his3-11,15 ura3-1 ade2-1 trp1-1 can1-100, REB1p-3xFLAG-MNase-URA3; MATa

value: Calcium

tag: time

value: 30 seconds after Calcium addition (Mnase activation)


title: ChEC_ifh1_150sec

status: Public on Mar 08 2019

type: SRA

country: Switzerland

library_source: genomic

molecule: genomic DNA

extract_protocol:
TAP and RNAPII ChIP-seq was performed as described previously (Ribaud et al., 2012) (PMID: 21952045). Chromatin for MNase digestion was prepared essentially as described in Kubik et al., 2015 (GSE73337) (PMID: 26545077).Cells were pelleted at 3,000 x g and washed three times with 1 mL Buffer A (15 mM Tris pH 7.5, 80 mM KCl, 0.1 mM EGTA, 0.2 mM spermine, 0.5 mM spermidine, Roche cOmplete EDTA-free mini protease inhibitors, 1 mM PMSF), centrifuging as above between washes. Cells were resuspended in 600 µL Buffer A containing 0.1% digitonin and permeabilized at 30C for 5 min. CaCl2 was added to 2 mM and ChEC digests were performed at 30C. 5 min after CaCl2 addition, a 100 µL aliquot of the digest was transferred to a tube containing 100 µL 2X stop buffer (400 mM NaCl, 20 mM EDTA, 4 mM EGTA) and 1% SDS. Protein was then was digested with 80 µg proteinase K at 55C for 30 m. Nucleic acids were extracted with an equal volume of phenol/chloroform/isoamyl alcohol (25:24:1) and precipitated with 2.5 volumes 100% ethanol and 30 µg Glycogen (Roche). Pellets were washed once with 1 mL 100% ethanol, dried, and resuspended in 30 µL 0.1X TE buffer, pH 8.0. RNA was digested with 10 µg RNase A at 37C for 20 m. Prior to generating sequencing libraries, ChEC DNA was subjected to size-selection using Ampure XP beads (ThermoFischer). A beads:sample ratio of 2.5:1 was used and the unbound fraction, containing small DNA fragments, was extracted to remove RNase and precipitated as above, and used for library preparation.
Libraries were prepared essentially as described in Henikoff et al., 2011 (GSE30551) (PMID: 22025707

tag: strain

tag: genotype

value: leu2-3,112 his3-11,15 ura3-1 ade2-1 trp1-1 can1-100, IFH1-3xFLAG-MNase-HPH; MATa

tag: treatment

value: 150 seconds after Calcium addition (Mnase activation)


title: EXP1_COL03_ROW12

city: New York

first: Kemal

middle: Marc

last: Akat

state: NY

address: 1230 York Avenue

country: USA

institute: The Rockefeller University

description:
exp1_counts.txt
exp1_spike_counts.txt

library_source: transcriptomic

data_processing:
Files were demultiplexed using Perl script mRNASeqHT_demultiplex.pl provided by Fluidigm
Demultiplexed FASTQ files were polyA trimmed using cutadapt (v 1.11)
polyA trimmed reads were aligned to the mouse genome using the STAR aligner (2.4.2a) allowing 2 mismatches and otherwise default parameters; if applicable, reads unmapped to the mouse genome were mapped to an ERCC spike-in reference using the BWA aligner (0.7.17-r1188) allowing 2 mismatches.
gene counting with featureCounts (1.5.1) counting multimappers fractionally
Genome_build: mm10

molecule: total RNA

organism: Mus musculus

source_name: single-cell suspension from whole glomerulus, kidney

extract_protocol:
Glomerular cells were isolated by perfusing mice with PBS, followed by Dynabead solution and then 2 ml bead solution with enzymatic digestion buffer containing collagenase type II 300U/ml, Pronase E (1mg/ml) and DNase I (50μg/ml). Kidneys were then removed, decapsulated, minced into 1 mm3 pieces, and digested in 3 ml digestion buffer at 37°C for 15 minutes on a rotator (100 rpm). Digested tissue was then passed through a 100 μm cell strainer and collected by centrifugation. The pellet was resuspended in 2 ml of Hank's buffered salt solution and glomeruli were washed three times and collected using a magnet. Separated glomeruli were resuspended in 2 ml digestion buffer and incubated at 37°C for an additional 40 minutes rotating on a thermomixer. During the digestion period, the solution was vortexed every 10 minutes and sheared with a 27G needle every 15 minutes. The solution was then put on magnetic particle concentrator, and supernatants were pooled. The suspension was sieved through a 40 μm cell strainer and centrifuged at 1,500rpm for 5 minutes at 4°C.
RNA libraries were prepared for sequencing using version 2 of the Fluidigm C1 800-cell IFC protocol

value: Control

tag: cell count

tag: age

value: Postnatal week 10


title: ChEC_ifh1_60sec

extract_protocol:
TAP and RNAPII ChIP-seq was performed as described previously (Ribaud et al., 2012) (PMID: 21952045). Chromatin for MNase digestion was prepared essentially as described in Kubik et al., 2015 (GSE73337) (PMID: 26545077).Cells were pelleted at 3,000 x g and washed three times with 1 mL Buffer A (15 mM Tris pH 7.5, 80 mM KCl, 0.1 mM EGTA, 0.2 mM spermine, 0.5 mM spermidine, Roche cOmplete EDTA-free mini protease inhibitors, 1 mM PMSF), centrifuging as above between washes. Cells were resuspended in 600 µL Buffer A containing 0.1% digitonin and permeabilized at 30C for 5 min. CaCl2 was added to 2 mM and ChEC digests were performed at 30C. 5 min after CaCl2 addition, a 100 µL aliquot of the digest was transferred to a tube containing 100 µL 2X stop buffer (400 mM NaCl, 20 mM EDTA, 4 mM EGTA) and 1% SDS. Protein was then was digested with 80 µg proteinase K at 55C for 30 m. Nucleic acids were extracted with an equal volume of phenol/chloroform/isoamyl alcohol (25:24:1) and precipitated with 2.5 volumes 100% ethanol and 30 µg Glycogen (Roche). Pellets were washed once with 1 mL 100% ethanol, dried, and resuspended in 30 µL 0.1X TE buffer, pH 8.0. RNA was digested with 10 µg RNase A at 37C for 20 m. Prior to generating sequencing libraries, ChEC DNA was subjected to size-selection using Ampure XP beads (ThermoFischer). A beads:sample ratio of 2.5:1 was used and the unbound fraction, containing small DNA fragments, was extracted to remove RNase and precipitated as above, and used for library preparation.
Libraries were prepared essentially as described in Henikoff et al., 2011 (GSE30551) (PMID: 22025709

value: 60 seconds after Calcium addition (Mnase activation)


title: ChEC_ifh1_30sec

extract_protocol:
TAP and RNAPII ChIP-seq was performed as described previously (Ribaud et al., 2012) (PMID: 21952045). Chromatin for MNase digestion was prepared essentially as described in Kubik et al., 2015 (GSE73337) (PMID: 26545077).Cells were pelleted at 3,000 x g and washed three times with 1 mL Buffer A (15 mM Tris pH 7.5, 80 mM KCl, 0.1 mM EGTA, 0.2 mM spermine, 0.5 mM spermidine, Roche cOmplete EDTA-free mini protease inhibitors, 1 mM PMSF), centrifuging as above between washes. Cells were resuspended in 600 µL Buffer A containing 0.1% digitonin and permeabilized at 30C for 5 min. CaCl2 was added to 2 mM and ChEC digests were performed at 30C. 5 min after CaCl2 addition, a 100 µL aliquot of the digest was transferred to a tube containing 100 µL 2X stop buffer (400 mM NaCl, 20 mM EDTA, 4 mM EGTA) and 1% SDS. Protein was then was digested with 80 µg proteinase K at 55C for 30 m. Nucleic acids were extracted with an equal volume of phenol/chloroform/isoamyl alcohol (25:24:1) and precipitated with 2.5 volumes 100% ethanol and 30 µg Glycogen (Roche). Pellets were washed once with 1 mL 100% ethanol, dried, and resuspended in 30 µL 0.1X TE buffer, pH 8.0. RNA was digested with 10 µg RNase A at 37C for 20 m. Prior to generating sequencing libraries, ChEC DNA was subjected to size-selection using Ampure XP beads (ThermoFischer). A beads:sample ratio of 2.5:1 was used and the unbound fraction, containing small DNA fragments, was extracted to remove RNase and precipitated as above, and used for library preparation.
Libraries were prepared essentially as described in Henikoff et al., 2011 (GSE30551) (PMID: 22025708


title: EXP1_COL03_ROW01

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL03_ROW10

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL02_ROW35

description:
exp1_counts.txt
exp1_spike_counts.txt


title: CHEC_sfp1_240sec_b

extract_protocol:
TAP and RNAPII ChIP-seq was performed as described previously (Ribaud et al., 2012) (PMID: 21952045). Chromatin for MNase digestion was prepared essentially as described in Kubik et al., 2015 (GSE73337) (PMID: 26545077).Cells were pelleted at 3,000 x g and washed three times with 1 mL Buffer A (15 mM Tris pH 7.5, 80 mM KCl, 0.1 mM EGTA, 0.2 mM spermine, 0.5 mM spermidine, Roche cOmplete EDTA-free mini protease inhibitors, 1 mM PMSF), centrifuging as above between washes. Cells were resuspended in 600 µL Buffer A containing 0.1% digitonin and permeabilized at 30C for 5 min. CaCl2 was added to 2 mM and ChEC digests were performed at 30C. 5 min after CaCl2 addition, a 100 µL aliquot of the digest was transferred to a tube containing 100 µL 2X stop buffer (400 mM NaCl, 20 mM EDTA, 4 mM EGTA) and 1% SDS. Protein was then was digested with 80 µg proteinase K at 55C for 30 m. Nucleic acids were extracted with an equal volume of phenol/chloroform/isoamyl alcohol (25:24:1) and precipitated with 2.5 volumes 100% ethanol and 30 µg Glycogen (Roche). Pellets were washed once with 1 mL 100% ethanol, dried, and resuspended in 30 µL 0.1X TE buffer, pH 8.0. RNA was digested with 10 µg RNase A at 37C for 20 m. Prior to generating sequencing libraries, ChEC DNA was subjected to size-selection using Ampure XP beads (ThermoFischer). A beads:sample ratio of 2.5:1 was used and the unbound fraction, containing small DNA fragments, was extracted to remove RNase and precipitated as above, and used for library preparation.
Libraries were prepared essentially as described in Henikoff et al., 2011 (GSE30551) (PMID: 22025704

value: leu2-3,112 his3-11,15 ura3-1 ade2-1 trp1-1 can1-100, SFP1--3xFLAG-MNase-HPH; MATa

value: 240 seconds after Calcium addition (Mnase activation)


title: CHEC_sfp1_30sec

extract_protocol:
TAP and RNAPII ChIP-seq was performed as described previously (Ribaud et al., 2012) (PMID: 21952045). Chromatin for MNase digestion was prepared essentially as described in Kubik et al., 2015 (GSE73337) (PMID: 26545077).Cells were pelleted at 3,000 x g and washed three times with 1 mL Buffer A (15 mM Tris pH 7.5, 80 mM KCl, 0.1 mM EGTA, 0.2 mM spermine, 0.5 mM spermidine, Roche cOmplete EDTA-free mini protease inhibitors, 1 mM PMSF), centrifuging as above between washes. Cells were resuspended in 600 µL Buffer A containing 0.1% digitonin and permeabilized at 30C for 5 min. CaCl2 was added to 2 mM and ChEC digests were performed at 30C. 5 min after CaCl2 addition, a 100 µL aliquot of the digest was transferred to a tube containing 100 µL 2X stop buffer (400 mM NaCl, 20 mM EDTA, 4 mM EGTA) and 1% SDS. Protein was then was digested with 80 µg proteinase K at 55C for 30 m. Nucleic acids were extracted with an equal volume of phenol/chloroform/isoamyl alcohol (25:24:1) and precipitated with 2.5 volumes 100% ethanol and 30 µg Glycogen (Roche). Pellets were washed once with 1 mL 100% ethanol, dried, and resuspended in 30 µL 0.1X TE buffer, pH 8.0. RNA was digested with 10 µg RNase A at 37C for 20 m. Prior to generating sequencing libraries, ChEC DNA was subjected to size-selection using Ampure XP beads (ThermoFischer). A beads:sample ratio of 2.5:1 was used and the unbound fraction, containing small DNA fragments, was extracted to remove RNase and precipitated as above, and used for library preparation.
Libraries were prepared essentially as described in Henikoff et al., 2011 (GSE30551) (PMID: 22025706


title: CHEC_free_mnase_20mn

extract_protocol:
TAP and RNAPII ChIP-seq was performed as described previously (Ribaud et al., 2012) (PMID: 21952045). Chromatin for MNase digestion was prepared essentially as described in Kubik et al., 2015 (GSE73337) (PMID: 26545077).Cells were pelleted at 3,000 x g and washed three times with 1 mL Buffer A (15 mM Tris pH 7.5, 80 mM KCl, 0.1 mM EGTA, 0.2 mM spermine, 0.5 mM spermidine, Roche cOmplete EDTA-free mini protease inhibitors, 1 mM PMSF), centrifuging as above between washes. Cells were resuspended in 600 µL Buffer A containing 0.1% digitonin and permeabilized at 30C for 5 min. CaCl2 was added to 2 mM and ChEC digests were performed at 30C. 5 min after CaCl2 addition, a 100 µL aliquot of the digest was transferred to a tube containing 100 µL 2X stop buffer (400 mM NaCl, 20 mM EDTA, 4 mM EGTA) and 1% SDS. Protein was then was digested with 80 µg proteinase K at 55C for 30 m. Nucleic acids were extracted with an equal volume of phenol/chloroform/isoamyl alcohol (25:24:1) and precipitated with 2.5 volumes 100% ethanol and 30 µg Glycogen (Roche). Pellets were washed once with 1 mL 100% ethanol, dried, and resuspended in 30 µL 0.1X TE buffer, pH 8.0. RNA was digested with 10 µg RNase A at 37C for 20 m. Prior to generating sequencing libraries, ChEC DNA was subjected to size-selection using Ampure XP beads (ThermoFischer). A beads:sample ratio of 2.5:1 was used and the unbound fraction, containing small DNA fragments, was extracted to remove RNase and precipitated as above, and used for library preparation.
Libraries were prepared essentially as described in Henikoff et al., 2011 (GSE30551) (PMID: 22025705

value: 20 minutes seconds after Calcium addition (Mnase activation)


title: EXP1_COL03_ROW02

description:
exp1_counts.txt
exp1_spike_counts.txt


title: CHEC_sfp1_150sec_b

extract_protocol:
TAP and RNAPII ChIP-seq was performed as described previously (Ribaud et al., 2012) (PMID: 21952045). Chromatin for MNase digestion was prepared essentially as described in Kubik et al., 2015 (GSE73337) (PMID: 26545077).Cells were pelleted at 3,000 x g and washed three times with 1 mL Buffer A (15 mM Tris pH 7.5, 80 mM KCl, 0.1 mM EGTA, 0.2 mM spermine, 0.5 mM spermidine, Roche cOmplete EDTA-free mini protease inhibitors, 1 mM PMSF), centrifuging as above between washes. Cells were resuspended in 600 µL Buffer A containing 0.1% digitonin and permeabilized at 30C for 5 min. CaCl2 was added to 2 mM and ChEC digests were performed at 30C. 5 min after CaCl2 addition, a 100 µL aliquot of the digest was transferred to a tube containing 100 µL 2X stop buffer (400 mM NaCl, 20 mM EDTA, 4 mM EGTA) and 1% SDS. Protein was then was digested with 80 µg proteinase K at 55C for 30 m. Nucleic acids were extracted with an equal volume of phenol/chloroform/isoamyl alcohol (25:24:1) and precipitated with 2.5 volumes 100% ethanol and 30 µg Glycogen (Roche). Pellets were washed once with 1 mL 100% ethanol, dried, and resuspended in 30 µL 0.1X TE buffer, pH 8.0. RNA was digested with 10 µg RNase A at 37C for 20 m. Prior to generating sequencing libraries, ChEC DNA was subjected to size-selection using Ampure XP beads (ThermoFischer). A beads:sample ratio of 2.5:1 was used and the unbound fraction, containing small DNA fragments, was extracted to remove RNase and precipitated as above, and used for library preparation.
Libraries were prepared essentially as described in Henikoff et al., 2011 (GSE30551) (PMID: 22025703


title: EXP1_COL02_ROW29

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL02_ROW27

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL02_ROW28

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL02_ROW22

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL03_ROW18

description:
exp1_counts.txt
exp1_spike_counts.txt


title: CHEC_sfp130sec_b

extract_protocol:
TAP and RNAPII ChIP-seq was performed as described previously (Ribaud et al., 2012) (PMID: 21952045). Chromatin for MNase digestion was prepared essentially as described in Kubik et al., 2015 (GSE73337) (PMID: 26545077).Cells were pelleted at 3,000 x g and washed three times with 1 mL Buffer A (15 mM Tris pH 7.5, 80 mM KCl, 0.1 mM EGTA, 0.2 mM spermine, 0.5 mM spermidine, Roche cOmplete EDTA-free mini protease inhibitors, 1 mM PMSF), centrifuging as above between washes. Cells were resuspended in 600 µL Buffer A containing 0.1% digitonin and permeabilized at 30C for 5 min. CaCl2 was added to 2 mM and ChEC digests were performed at 30C. 5 min after CaCl2 addition, a 100 µL aliquot of the digest was transferred to a tube containing 100 µL 2X stop buffer (400 mM NaCl, 20 mM EDTA, 4 mM EGTA) and 1% SDS. Protein was then was digested with 80 µg proteinase K at 55C for 30 m. Nucleic acids were extracted with an equal volume of phenol/chloroform/isoamyl alcohol (25:24:1) and precipitated with 2.5 volumes 100% ethanol and 30 µg Glycogen (Roche). Pellets were washed once with 1 mL 100% ethanol, dried, and resuspended in 30 µL 0.1X TE buffer, pH 8.0. RNA was digested with 10 µg RNase A at 37C for 20 m. Prior to generating sequencing libraries, ChEC DNA was subjected to size-selection using Ampure XP beads (ThermoFischer). A beads:sample ratio of 2.5:1 was used and the unbound fraction, containing small DNA fragments, was extracted to remove RNase and precipitated as above, and used for library preparation.
Libraries were prepared essentially as described in Henikoff et al., 2011 (GSE30551) (PMID: 22025700


title: EXP1_COL02_ROW17

description:
exp1_counts.txt
exp1_spike_counts.txt


title: ChIP_Sfp1_TAP_raf

data_processing:
For ChIP-Seq raw reads were aligned to the sacCer3 and 972-h pombe genome assembly using Bowtie2 with options "-k 20 --end-to-end --sensitive -X 800".
For ChIP-Seq, data were filtered to retain only the mapped reads with at most 5 hits in the reference.
For ChIP-Seq, reads that mapped to both sacCer3 and 972-h genomes were discarded.
For ChIP-Seq, the reads were shifted by 100 bp and extended by 50 bp
For ChIP-Seq, reads mapped uniquely to the sacCer3 genome were first normalized to total number of mapped reads in sacCer3 and then to total number of uniquely mapped reads in 972-h pombe in the same sample as described in (Hu et al. 2015)
For ChEC-Seq, Pairs of FASTQ files were mapped to the sacCer3 genome assembly using Novoalign (Novocraft), generating SAM files that were then converted to tag directories with HOMER (PMID 20513432) (homer.ucsd.edu) for further analysis.
Genome_build: sacCer3, 972-h pombe
Supplementary_files_format_and_content: wig and bigwig files were obtained using HTSStation (http://htsstation.epfl.ch)
Supplementary_files_format_and_content: ChEC-seq data were visualized as reads per million (RPM)-normalized single-base resolution bedgraph tracks.

extract_protocol:
TAP and RNAPII ChIP-seq was performed as described previously (Ribaud et al., 2012) (PMID: 21952045). Chromatin for MNase digestion was prepared essentially as described in Kubik et al., 2015 (GSE73337) (PMID: 26545077).Cells were pelleted at 3,000 x g and washed three times with 1 mL Buffer A (15 mM Tris pH 7.5, 80 mM KCl, 0.1 mM EGTA, 0.2 mM spermine, 0.5 mM spermidine, Roche cOmplete EDTA-free mini protease inhibitors, 1 mM PMSF), centrifuging as above between washes. Cells were resuspended in 600 µL Buffer A containing 0.1% digitonin and permeabilized at 30C for 5 min. CaCl2 was added to 2 mM and ChEC digests were performed at 30C. 5 min after CaCl2 addition, a 100 µL aliquot of the digest was transferred to a tube containing 100 µL 2X stop buffer (400 mM NaCl, 20 mM EDTA, 4 mM EGTA) and 1% SDS. Protein was then was digested with 80 µg proteinase K at 55C for 30 m. Nucleic acids were extracted with an equal volume of phenol/chloroform/isoamyl alcohol (25:24:1) and precipitated with 2.5 volumes 100% ethanol and 30 µg Glycogen (Roche). Pellets were washed once with 1 mL 100% ethanol, dried, and resuspended in 30 µL 0.1X TE buffer, pH 8.0. RNA was digested with 10 µg RNase A at 37C for 20 m. Prior to generating sequencing libraries, ChEC DNA was subjected to size-selection using Ampure XP beads (ThermoFischer). A beads:sample ratio of 2.5:1 was used and the unbound fraction, containing small DNA fragments, was extracted to remove RNase and precipitated as above, and used for library preparation.
TruSeq ChIP library preparation kit (Illumina)

tag: chip antibody

value: Anti-TAP thermoscientific TAP CAB1001


title: ChIP_TBP_20mn_sfp1AA_rapa

value: TBP-MYC::G418,tor1-1 fpr1{delta}::Nat RPL13A-2XFKB12::TRP1 SFP1-FRB-KanMX MAT{alpha}

value: Rapamycin

value: Anti-MYC

value: 20min after treatment


title: EXP1_COL02_ROW15

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL02_ROW13

description:
exp1_counts.txt
exp1_spike_counts.txt


title: ChIP_sfp1_TAP_GLU

value: GLUCOSE 2%


title: EXP1_COL02_ROW12

description:
exp1_counts.txt
exp1_spike_counts.txt


title: ChIP_TAP_ctrl


title: EXP1_COL02_ROW01

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL01_ROW40

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL02_ROW11

description:
exp1_counts.txt
exp1_spike_counts.txt


title: Rpb1_ChIP_RAF

value: Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) (ab5131)


title: EXP1_COL01_ROW37

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL02_ROW31

description:
exp1_counts.txt
exp1_spike_counts.txt


title: CHEC_sfp1_60sec_b

extract_protocol:
TAP and RNAPII ChIP-seq was performed as described previously (Ribaud et al., 2012) (PMID: 21952045). Chromatin for MNase digestion was prepared essentially as described in Kubik et al., 2015 (GSE73337) (PMID: 26545077).Cells were pelleted at 3,000 x g and washed three times with 1 mL Buffer A (15 mM Tris pH 7.5, 80 mM KCl, 0.1 mM EGTA, 0.2 mM spermine, 0.5 mM spermidine, Roche cOmplete EDTA-free mini protease inhibitors, 1 mM PMSF), centrifuging as above between washes. Cells were resuspended in 600 µL Buffer A containing 0.1% digitonin and permeabilized at 30C for 5 min. CaCl2 was added to 2 mM and ChEC digests were performed at 30C. 5 min after CaCl2 addition, a 100 µL aliquot of the digest was transferred to a tube containing 100 µL 2X stop buffer (400 mM NaCl, 20 mM EDTA, 4 mM EGTA) and 1% SDS. Protein was then was digested with 80 µg proteinase K at 55C for 30 m. Nucleic acids were extracted with an equal volume of phenol/chloroform/isoamyl alcohol (25:24:1) and precipitated with 2.5 volumes 100% ethanol and 30 µg Glycogen (Roche). Pellets were washed once with 1 mL 100% ethanol, dried, and resuspended in 30 µL 0.1X TE buffer, pH 8.0. RNA was digested with 10 µg RNase A at 37C for 20 m. Prior to generating sequencing libraries, ChEC DNA was subjected to size-selection using Ampure XP beads (ThermoFischer). A beads:sample ratio of 2.5:1 was used and the unbound fraction, containing small DNA fragments, was extracted to remove RNase and precipitated as above, and used for library preparation.
Libraries were prepared essentially as described in Henikoff et al., 2011 (GSE30551) (PMID: 22025702

value: Calcium


title: ChIP_TBP_20mn_sfp1AA_veh

value: no Rapamycin


title: EXP1_COL01_ROW33

description:
exp1_counts.txt
exp1_spike_counts.txt


title: ChIP_Sfp1_TAP_gal


title: Rpb1_ChIP_Sfp1OE_t1h

value: GAL1p-SFP1-TAP::URA3 MATa

value: 60min after treatment


title: Rpb1_ChIP_Sfp1OE_t0

value: 0min after treatment


title: EXP1_COL01_ROW34

description:
exp1_counts.txt
exp1_spike_counts.txt

value: Control

value: Postnatal week 10


title: EXP1_COL02_ROW18

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL01_ROW32

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL01_ROW31

description:
exp1_counts.txt
exp1_spike_counts.txt


title: Rpb1_ChIP_Sfp1_AA_t20

value: tor1-1 fpr1{delta}::Nat RPL13A-2XFKB12::TRP1 SFP1-FRB-KanMX MAT{alpha}


title: Rpb1_ChIP_Sfp1_AA_t60


title: ChIP_SFP1_OE_t0


title: EXP1_COL01_ROW30

description:
exp1_counts.txt
exp1_spike_counts.txt


title: Rpb1_ChIP_Sfp1_AA_t0


title: ChIP_SFP1_OE_t1h


title: Rpb1_ChIP_wt_t60

value: tor1-1 fpr1{delta}::Nat RPL13A-2xFKBP12-TRP1 MAT{alpha}


title: EXP1_COL01_ROW29

description:
exp1_counts.txt
exp1_spike_counts.txt


title: CHEC_sfp1_150sec

extract_protocol:
TAP and RNAPII ChIP-seq was performed as described previously (Ribaud et al., 2012) (PMID: 21952045). Chromatin for MNase digestion was prepared essentially as described in Kubik et al., 2015 (GSE73337) (PMID: 26545077).Cells were pelleted at 3,000 x g and washed three times with 1 mL Buffer A (15 mM Tris pH 7.5, 80 mM KCl, 0.1 mM EGTA, 0.2 mM spermine, 0.5 mM spermidine, Roche cOmplete EDTA-free mini protease inhibitors, 1 mM PMSF), centrifuging as above between washes. Cells were resuspended in 600 µL Buffer A containing 0.1% digitonin and permeabilized at 30C for 5 min. CaCl2 was added to 2 mM and ChEC digests were performed at 30C. 5 min after CaCl2 addition, a 100 µL aliquot of the digest was transferred to a tube containing 100 µL 2X stop buffer (400 mM NaCl, 20 mM EDTA, 4 mM EGTA) and 1% SDS. Protein was then was digested with 80 µg proteinase K at 55C for 30 m. Nucleic acids were extracted with an equal volume of phenol/chloroform/isoamyl alcohol (25:24:1) and precipitated with 2.5 volumes 100% ethanol and 30 µg Glycogen (Roche). Pellets were washed once with 1 mL 100% ethanol, dried, and resuspended in 30 µL 0.1X TE buffer, pH 8.0. RNA was digested with 10 µg RNase A at 37C for 20 m. Prior to generating sequencing libraries, ChEC DNA was subjected to size-selection using Ampure XP beads (ThermoFischer). A beads:sample ratio of 2.5:1 was used and the unbound fraction, containing small DNA fragments, was extracted to remove RNase and precipitated as above, and used for library preparation.
Libraries were prepared essentially as described in Henikoff et al., 2011 (GSE30551) (PMID: 22025701

value: leu2-3,112 his3-11,15 ura3-1 ade2-1 trp1-1 can1-100, SFP1--3xFLAG-MNase-HPH; MATa

value: 150 seconds after Calcium addition(Mnase activation)


title: Rpb1_ChIP_GAL


title: Rpb1_ChIP_wt_t20


title: EXP1_COL01_ROW35

description:
exp1_counts.txt
exp1_spike_counts.txt


title: Rpb1_ChIP_wt_t0


title: Rpb1_ChIP_GLUC


title: Rpb1_ChIP_wt_t5

value: 5min after treatment


title: EXP1_COL01_ROW19

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL01_ROW14

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL01_ROW25

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL01_ROW21

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL01_ROW13

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL01_ROW12

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH9968_ME_8mer_10670

type: protein

city: Toronto

first: Samuel

last: Lambert

state: ON

address: 160 College St.

department: Donnelly Centre for Cellular and Biomolecular Research

country: Canada

institute: University of Toronto

hyb_protocol: After a 2-h incubation at 37oC, 12.5 ml of the mix was added to 137.5 ml of protein-binding solution for a final mix of PBS/2% skim milk/0.2 mg per ml BSA/50 μM zinc acetate/0.1% Tween-20. This mixture was added to an array previously blocked with PBS/2% skim milk and washed once with PBS/0.1% Tween-20 and once with PBS/0.01% Triton-X 100. After a 1-h incubation at room temperature, the array was washed once with PBS/0.5% Tween-20/50 mM zinc acetate and once with PBS/0.01% Triton-X 100/50 mM zinc acetate. Cy5-labeled anti-GST antibody was added, diluted in PBS/2% skim milk/50 mM zinc acetate. After a 1-h incubation at room temperature, the array was washed three times with PBS/0.05% Tween-20/50 mM zinc acetate and once with PBS/50 mMzinc acetate.

scan_protocol: The array was imaged using an Agilent microarray scanner at 2 mM resolution. Images were scanned at two power settings: 100% photomultiplier tube (PMT) voltage (high), and 10% PMT (low). The two resulting grid images were then manually examined, and the scan with the fewest number of saturated spots was used. Image spot intensities were quantified using ImaGene software (BioDiscovery).

data_processing: We provide several scores for each 8-mer in each experiment. The VALUE column contains the median intensity for the 8mer sequence. Z-Score – transformed kmer median intensity; E-score – Enrichment Score. E-scores are a modified version of AUC, and describe how well each kmer ranks the intensities of the spots. Please see the supplementary files on the platform record (GPL11260) to match the raw data files with the array probes.

molecule: protein

organism: synthetic construct

source_name: KNAT4, ME design

label_protocol: We used 150 ng of plasmid DNA in a 15 μl in vitro transcription/ translation reaction using a PURExpress In Vitro Protein Synthesis Kit (New England BioLabs) supplemented with RNase inhibitor and 50 μM zinc acetate.

extract_protocol: DBD sequences along with 50 amino acid residues on either side of the DBD in the native protein were cloned as AscI–SbfI fragments into pTH6838, a modified T7-driven GST expression vector.

value: Homeobox

value: ME


title: Rpb1_ChIP_Sfp1_AA_t5

value: Rapamycin


title: EXP1_COL01_ROW07

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL01_ROW10

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH9968_HK_8mer_10686

source_name: KNAT4, HK design

value: HK


title: pTH9967_HK_8mer_10685

source_name: UNE16, HK design


title: pTH9963_ME_8mer_10665

source_name: TRP5, ME design


title: pTH9963_HK_8mer_10681

source_name: TRP5, HK design


title: EXP1_COL01_ROW05

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL01_ROW01

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL01_ROW03

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH9962_ME_8mer_10664

source_name: anac028, ME design

value: NAM


title: EXP1_COL01_ROW27

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH9967_ME_8mer_10669

source_name: UNE16, ME design


title: pTH9959_ME_8mer_10661

source_name: HYH, ME design


title: EXP2_COL20_ROW35


title: EXP2_COL20_ROW28


title: pTH9959_HK_8mer_10677

source_name: HYH, HK design


title: pTH9962_HK_8mer_10680

source_name: anac028, HK design


title: EXP2_COL20_ROW24


title: EXP2_COL20_ROW25


title: pTH9944_HK_8mer_11922

source_name: nhr-6, HK design


title: EXP2_COL20_ROW23


title: pTH9942_HK_8mer_10610

source_name: 1600010210_CONSTRUCT, HK design

value: GATA


title: pTH9942_ME_8mer_10549

source_name: 1600010210_CONSTRUCT, ME design


title: EXP2_COL20_ROW10


title: pTH9941_HK_8mer_10609

source_name: CEG01538.1, HK design


title: pTH9845_ME_8mer_10658

source_name: NFE2L2, ME design


title: EXP2_COL20_ROW09


title: EXP2_COL20_ROW05


title: EXP2_COL20_ROW04


title: pTH9941_ME_8mer_10548

source_name: CEG01538.1, ME design


title: EXP2_COL20_ROW33

source_name: single-cell suspension from whole glomerulus, kidney


title: EXP2_COL19_ROW33


title: pTH9944_ME_8mer_8912

source_name: nhr-6, ME design


title: EXP2_COL19_ROW35


title: EXP2_COL19_ROW24


title: pTH9845_HK_8mer_10674

source_name: NFE2L2, HK design


title: EXP2_COL20_ROW03


title: EXP2_COL19_ROW17


title: EXP2_COL20_ROW20


title: EXP2_COL19_ROW05


title: pTH9839_HK_8mer_8444

source_name: SCHCODRAFT_110010, HK design


title: pTH9833_ME_8mer_10657

source_name: bnl, ME design


title: pTH9835_HK_8mer_8440

value: TCR


title: pTH9839_ME_8mer_8460

source_name: SCHCODRAFT_110010, ME design


title: pTH9835_ME_8mer_8456


title: EXP2_COL19_ROW07


title: pTH9831_HK_8mer_8438

source_name: ENSXETG00000015341_CONSTRUCT, HK design


title: EXP2_COL19_ROW02


title: pTH9830_HK_8mer_9645

source_name: ENSTNIG00000009360_CONSTRUCT, HK design

value: BrkDBD


title: EXP2_COL20_ROW02


title: pTH9824_ME_8mer_8429

source_name: foxe3, ME design

value: Forkhead


title: pTH9838_ME_8mer_8459

value: KilA-N


title: EXP2_COL18_ROW33

value: Control


title: EXP2_COL18_ROW34

value: Postnatal week 10


title: EXP2_COL19_ROW04


title: pTH9833_HK_8mer_10673

source_name: bnl, HK design


title: pTH9838_HK_8mer_8443


title: pTH9824_HK_8mer_10617

source_name: foxe3, HK design


title: EXP2_COL18_ROW31


title: EXP2_COL18_ROW28


title: pTH9831_ME_8mer_8454

source_name: ENSXETG00000015341_CONSTRUCT, ME design


title: pTH9820_ME_8mer_9631


title: pTH9820_HK_8mer_9983


title: EXP2_COL19_ROW10


title: EXP2_COL18_ROW18


title: pTH9809_ME_8mer_8335

source_name: estExt_gwp_gw1.C_220046, ME design


title: EXP2_COL18_ROW20


title: EXP2_COL18_ROW19


title: EXP2_COL18_ROW25


title: pTH9816_ME_8mer_9629


title: EXP2_COL18_ROW39


title: pTH9816_HK_8mer_9981


title: EXP2_COL18_ROW13


title: pTH9815_HK_8mer_10622

source_name: ENSTNIG00000008857_CONSTRUCT, HK design

value: TEA


title: pTH9815_ME_8mer_10974

source_name: ENSTNIG00000008857_CONSTRUCT, ME design


title: pTH9808_HK_8mer_10620

source_name: NAIF1_CHICK, HK design


title: pTH9830_ME_8mer_10975

source_name: ENSTNIG00000009360_CONSTRUCT, ME design


title: EXP2_COL18_ROW03

source_name: single-cell suspension from whole glomerulus, kidney


title: EXP2_COL18_ROW16


title: pTH9809_HK_8mer_10618

source_name: estExt_gwp_gw1.C_220046, HK design


title: pTH9799_ME_8mer_8178

source_name: dmrt99B, ME design

value: DM


title: EXP2_COL17_ROW36


title: pTH9808_ME_8mer_8334

source_name: NAIF1_CHICK, ME design


title: EXP2_COL18_ROW02


title: EXP2_COL17_ROW34


title: EXP2_COL18_ROW10


title: EXP2_COL18_ROW07


title: EXP2_COL18_ROW04


title: EXP2_COL17_ROW30


title: EXP2_COL17_ROW14


title: pTH9799_HK_8mer_8250

source_name: dmrt99B, HK design


title: pTH9781_ME_8mer_8160

source_name: Pax5B, ME design

value: PAX


title: pTH9776_HK_8mer_8289

source_name: SCHCODRAFT_67234, HK design


title: pTH9781_HK_8mer_8246

source_name: Pax5B, HK design


title: pTH9773_ME_8mer_8151

source_name: sv, ME design


title: EXP2_COL17_ROW20


title: pTH9776_ME_8mer_8194

source_name: SCHCODRAFT_67234, ME design


title: pTH9772_ME_8mer_8150

source_name: NEMVEDRAFT_v1g112929, ME design

value: Pou,Homeobox


title: EXP2_COL17_ROW08


title: pTH9772_HK_8mer_8243

source_name: NEMVEDRAFT_v1g112929, HK design


title: pTH9802_HK_8mer_8322

source_name: ANIA_01906, HK design


title: EXP2_COL16_ROW38


title: EXP2_COL17_ROW09


title: pTH9773_HK_8mer_8244

source_name: sv, HK design


title: pTH9802_ME_8mer_8276

source_name: ANIA_01906, ME design


title: pTH9771_ME_8mer_8149

source_name: NEMVEDRAFT_v1g111410, ME design


title: pTH9771_HK_8mer_8242

source_name: NEMVEDRAFT_v1g111410, HK design


title: pTH9770_HK_8mer_8241

source_name: NEMVEDRAFT_v1g160868, HK design


title: pTH9770_ME_8mer_8148

source_name: NEMVEDRAFT_v1g160868, ME design


title: EXP2_COL16_ROW31

source_name: single-cell suspension from whole glomerulus, kidney


title: EXP2_COL16_ROW27


title: EXP2_COL16_ROW33


title: EXP2_COL16_ROW35


title: EXP2_COL16_ROW25


title: EXP2_COL16_ROW20


title: EXP2_COL16_ROW18


title: EXP2_COL17_ROW13


title: pTH9768_ME_8mer_11005

source_name: NEMVEDRAFT_v1g104083, ME design

value: CSD


title: pTH9764_ME_8mer_8188

source_name: Runx2, ME design

value: Runt


title: pTH9764_HK_8mer_8316

source_name: Runx2, HK design


title: EXP2_COL16_ROW17


title: pTH9755_ME_8mer_7983

source_name: ENSXETG00000008236_CONSTRUCT, ME design

value: ME


title: EXP2_COL16_ROW09


title: pTH9768_HK_8mer_10983

source_name: NEMVEDRAFT_v1g104083, HK design


title: EXP2_COL16_ROW08


title: pTH9755_HK_8mer_8111

source_name: ENSXETG00000008236_CONSTRUCT, HK design


title: EXP2_COL16_ROW14


title: pTH9715_ME_8mer_11968

source_name: pie-1, ME design

value: zf-CCCH


title: EXP2_COL16_ROW05


title: pTH9715_HK_8mer_8762

source_name: pie-1, HK design

value: HK


title: EXP2_COL16_ROW06


title: EXP2_COL16_ROW03


title: pTH9706_HK_8mer_7639

source_name: wc-1, HK design


title: EXP2_COL16_ROW04


title: pTH9692_ME_8mer_7744

source_name: NCU01629, ME design


title: EXP2_COL15_ROW16


title: EXP2_COL15_ROW15


title: pTH9706_ME_8mer_7467

source_name: wc-1, ME design


title: pTH9684_ME_8mer_11967

source_name: NCU07379, ME design


title: EXP2_COL16_ROW01


title: pTH9692_HK_8mer_7966

source_name: NCU01629, HK design


title: pTH9684_HK_8mer_7965

source_name: NCU07379, HK design


title: EXP2_COL15_ROW22


title: pTH9680_ME_8mer_7728

source_name: sah-2, ME design

value: HLH


title: EXP2_COL15_ROW12


title: EXP2_COL15_ROW14


title: EXP2_COL15_ROW08


title: pTH9679_ME_8mer_11966

source_name: NCU04211, ME design


title: EXP2_COL14_ROW40

source_name: single-cell suspension from whole glomerulus, kidney


title: pTH9679_HK_8mer_8315

source_name: NCU04211, HK design


title: pTH9659_ME_8mer_7706

source_name: ada-1, ME design


title: pTH9659_HK_8mer_7755

source_name: ada-1, HK design


title: EXP2_COL14_ROW33


title: EXP2_COL14_ROW35


title: pTH9347_ME_8mer_7533

source_name: NFATC4, ME design


title: pTH9347_HK_8mer_11022

source_name: NFATC4, HK design


title: EXP2_COL14_ROW38


title: EXP2_COL14_ROW26


title: EXP2_COL14_ROW24


title: EXP2_COL14_ROW20


title: pTH9144_ME_8mer_8647

source_name: ceh-74, ME design

value: Homeobox


title: EXP2_COL14_ROW21


title: pTH9309_HK_8mer_10989

source_name: PHF21A, HK design


title: pTH9144_HK_8mer_10755

source_name: ceh-74, HK design


title: EXP2_COL14_ROW18


title: EXP2_COL14_ROW16


title: EXP2_COL14_ROW17


title: pTH8939_ME_8mer_6406

source_name: nuc-1, ME design


title: pTH8934_ME_8mer_8237

source_name: col-24, ME design


title: pTH8939_HK_8mer_6461

source_name: nuc-1, HK design


title: pTH8934_HK_8mer_8312

source_name: col-24, HK design


title: pTH9680_HK_8mer_7771

source_name: sah-2, HK design


title: EXP2_COL14_ROW12


title: EXP2_COL14_ROW15


title: pTH8929_ME_8mer_6355

source_name: col-22, ME design


title: EXP2_COL14_ROW10


title: EXP2_COL14_ROW11


title: pTH8926_ME_8mer_8221

source_name: NCU03417, ME design


title: EXP2_COL13_ROW34


title: pTH8926_HK_8mer_6453

source_name: NCU03417, HK design


title: pTH9309_ME_8mer_6960

source_name: PHF21A, ME design


title: pTH8923_HK_8mer_6452

source_name: msn-1, HK design


title: pTH8929_HK_8mer_6454

source_name: col-22, HK design


title: pTH8923_ME_8mer_8222

source_name: msn-1, ME design


title: pTH8920_ME_8mer_6377

source_name: NCU02182, ME design


title: EXP2_COL13_ROW12

source_name: single-cell suspension from whole glomerulus, kidney


title: EXP2_COL13_ROW11


title: pTH8920_HK_8mer_11920

source_name: NCU02182, HK design


title: pTH8918_ME_8mer_6264

source_name: asl-1, ME design


title: pTH8918_HK_8mer_6322

source_name: asl-1, HK design


title: EXP2_COL13_ROW06


title: EXP2_COL13_ROW08


title: EXP2_COL13_ROW09


title: EXP2_COL13_ROW02


title: EXP2_COL13_ROW01


title: pTH8909_HK_8mer_6448

source_name: nit-4, HK design


title: pTH8908_HK_8mer_8260

source_name: NCU07834, HK design


title: pTH8908_ME_8mer_8330

source_name: NCU07834, ME design


title: EXP2_COL12_ROW36


title: pTH8909_ME_8mer_8223

source_name: nit-4, ME design


title: EXP2_COL12_ROW40


title: pTH8897_ME_8mer_11964

source_name: NCU06324, ME design


title: EXP2_COL12_ROW30


title: pTH8897_HK_8mer_6442

source_name: NCU06324, HK design


title: EXP2_COL12_ROW26


title: EXP2_COL12_ROW23


title: EXP2_COL13_ROW17


title: EXP2_COL12_ROW21


title: EXP2_COL13_ROW28


title: pTH8895_HK_8mer_12078

source_name: NCU05909, HK design


title: pTH8895_ME_8mer_12201

source_name: NCU05909, ME design


title: pTH8862_ME_8mer_12200

source_name: NCU05414, ME design


title: pTH8861_ME_8mer_12199

source_name: NCU03643, ME design


title: pTH8862_HK_8mer_12077

source_name: NCU05414, HK design


title: EXP2_COL12_ROW13


title: EXP2_COL12_ROW17


title: pTH8861_HK_8mer_12076

source_name: NCU03643, HK design


title: pTH8827_ME_8mer_6048

source_name: NCU08999, ME design


title: EXP2_COL12_ROW12

source_name: single-cell suspension from whole glomerulus, kidney


title: EXP2_COL12_ROW08


title: pTH8822_ME_8mer_8085

source_name: NCU05061, ME design


title: EXP2_COL12_ROW07


title: pTH8827_HK_8mer_6100

source_name: NCU08999, HK design


title: EXP2_COL12_ROW06


title: pTH8822_HK_8mer_11919

source_name: NCU05061, HK design


title: EXP2_COL11_ROW10


title: EXP2_COL12_ROW04


title: pTH8818_HK_8mer_8107

source_name: NCU05637, HK design


title: pTH8797_ME_8mer_6017

source_name: NCU03905, ME design


title: pTH8797_HK_8mer_6070

source_name: NCU03905, HK design


title: EXP2_COL11_ROW23


title: pTH8818_ME_8mer_8071

source_name: NCU05637, ME design


title: EXP2_COL11_ROW28


title: EXP2_COL10_ROW34

value: Diabetes


title: pTH8740_ME_8mer_8225

source_name: TB1, ME design

value: TCP


title: EXP2_COL12_ROW18


title: EXP2_COL10_ROW23


title: EXP2_COL10_ROW17


title: pTH8711_HK_8mer_6002

source_name: cre-1, HK design


title: pTH8711_ME_8mer_5915

source_name: cre-1, ME design


title: pTH8740_HK_8mer_8290

source_name: TB1, HK design


title: EXP2_COL10_ROW33


title: pTH8622_ME_8mer_5885

source_name: NCU03043, ME design


title: EXP2_COL10_ROW08


title: EXP2_COL10_ROW09


title: pTH8622_HK_8mer_5898

source_name: NCU03043, HK design


title: EXP2_COL10_ROW03


title: EXP2_COL10_ROW04


title: pTH8620_ME_8mer_5563

source_name: ada-3, ME design


title: pTH8620_HK_8mer_5712

source_name: ada-3, HK design


title: EXP2_COL10_ROW02


title: pTH8619_HK_8mer_5711

source_name: vad-2, HK design


title: EXP2_COL09_ROW30

source_name: single-cell suspension from whole glomerulus, kidney


title: pTH8619_ME_8mer_5562

source_name: vad-2, ME design


title: EXP2_COL09_ROW23


title: pTH8617_ME_8mer_5592

source_name: NCU01856, ME design


title: pTH8617_HK_8mer_5855

source_name: NCU01856, HK design


title: EXP2_COL08_ROW36


title: EXP2_COL09_ROW18


title: EXP2_COL09_ROW20


title: EXP2_COL09_ROW17


title: EXP2_COL08_ROW34


title: EXP2_COL08_ROW19


title: EXP2_COL08_ROW06


title: EXP2_COL08_ROW15


title: EXP2_COL08_ROW04


title: EXP2_COL08_ROW02


title: EXP2_COL07_ROW32


title: pTH8614_HK_8mer_5706

source_name: sub-1, HK design

value: GATA


title: pTH8614_ME_8mer_5606

source_name: sub-1, ME design


title: pTH8612_ME_8mer_12198

source_name: NCU01074, ME design


title: EXP2_COL07_ROW30


title: pTH8612_HK_8mer_12074

source_name: NCU01074, HK design


title: EXP2_COL07_ROW29


title: pTH7807_HK_8mer_5959

source_name: Smp_097750, HK design


title: pTH7599_ME_8mer_4343

source_name: TGA1, ME design


title: pTH5526_ME_8mer_820

source_name: gtaJ, ME design


title: pTH5526_HK_8mer_11982

source_name: gtaJ, HK design


title: pTH5339_ME_8mer_319

source_name: Osr1, ME design


title: pTH5530_ME_8mer_814

source_name: gtaS, ME design


title: EXP2_COL07_ROW28


title: pTH5530_HK_8mer_11918

source_name: gtaS, HK design


title: pTH7807_ME_8mer_5801

source_name: Smp_097750, ME design


title: pTH4990_HK_8mer_11914


title: EXP2_COL07_ROW19


title: pTH4990_ME_8mer_59


title: pTH3511_HK_8mer_11980

source_name: Esr2, HK design


title: EXP2_COL07_ROW13

source_name: single-cell suspension from whole glomerulus, kidney


title: EXP2_COL07_ROW15


title: pTH7599_HK_8mer_11396

source_name: TGA1, HK design


title: EXP2_COL07_ROW37


title: pTH3511_ME_8mer_1312

source_name: Esr2, ME design


title: EXP2_COL07_ROW20


title: pTH11853_HK_8mer_12205

source_name: clr-2, HK design


title: pTH11849_ME_8mer_12186

source_name: NCU10697, ME design


title: pTH11849_HK_8mer_12202

source_name: NCU10697, HK design


title: EXP2_COL06_ROW37


title: EXP2_COL07_ROW01


title: pTH11832_HK_8mer_12178

source_name: sre, HK design


title: EXP2_COL06_ROW35


title: pTH5339_HK_8mer_11981

source_name: Osr1, HK design


title: EXP2_COL06_ROW17


title: EXP2_COL07_ROW05


title: pTH11853_ME_8mer_12189

source_name: clr-2, ME design


title: EXP2_COL07_ROW08


title: EXP2_COL06_ROW23


title: EXP2_COL07_ROW09


title: pTH11830_ME_8mer_12160

source_name: NCU03421, ME design


title: pTH11830_HK_8mer_12176

source_name: NCU03421, HK design


title: EXP2_COL06_ROW12


title: EXP2_COL06_ROW14


title: pTH11569_ME_8mer_12364

source_name: TCP8, ME design


title: pTH11569_HK_8mer_12380

source_name: TCP8, HK design


title: pTH11852_ME_8mer_12188

source_name: col-26, ME design


title: EXP2_COL06_ROW08


title: EXP2_COL06_ROW10


title: EXP2_COL05_ROW40


title: pTH11832_ME_8mer_12162

source_name: sre, ME design


title: pTH11852_HK_8mer_12204

source_name: col-26, HK design


title: pTH11563_HK_8mer_12535

source_name: AT3G04930, HK design


title: EXP2_COL05_ROW39

source_name: single-cell suspension from whole glomerulus, kidney


title: pTH11563_ME_8mer_12412

source_name: AT3G04930, ME design


title: pTH11562_ME_8mer_12280

source_name: DOF2.5, ME design

value: zf-Dof


title: pTH11562_HK_8mer_12375

source_name: DOF2.5, HK design


title: pTH11560_HK_8mer_12374

source_name: SPL9, HK design

value: SBP


title: EXP2_COL05_ROW31

value: Postnatal week 10


title: EXP2_COL06_ROW16


title: EXP2_COL05_ROW19


title: pTH11557_ME_8mer_12276

source_name: AT1G14600, ME design


title: EXP2_COL05_ROW24


title: EXP2_COL05_ROW34


title: pTH11557_HK_8mer_12373

source_name: AT1G14600, HK design


title: pTH11556_ME_8mer_12275

source_name: MYB124, ME design


title: pTH11560_ME_8mer_12278

source_name: SPL9, ME design


title: pTH11556_HK_8mer_12372

source_name: MYB124, HK design


title: EXP2_COL04_ROW26


title: EXP2_COL05_ROW02


title: EXP2_COL05_ROW09


title: EXP2_COL05_ROW05


title: EXP2_COL04_ROW15


title: EXP2_COL04_ROW24


title: EXP2_COL04_ROW20


title: pTH11538_HK_8mer_12149

source_name: AT3G10030, HK design


title: pTH11551_HK_8mer_12371

source_name: ERF119, HK design


title: EXP2_COL04_ROW05


title: pTH11541_HK_8mer_12216

source_name: TINY, HK design


title: pTH11541_ME_8mer_12169

source_name: TINY, ME design


title: pTH11538_ME_8mer_12168

source_name: AT3G10030, ME design


title: EXP2_COL04_ROW08


title: pTH11535_ME_8mer_12164

source_name: AT1G49010, ME design


title: pTH11535_HK_8mer_12180

source_name: AT1G49010, HK design


title: EXP2_COL03_ROW40

source_name: single-cell suspension from whole glomerulus, kidney


title: pTH11534_HK_8mer_12145

source_name: ERF019, HK design


title: pTH11534_ME_8mer_12167

source_name: ERF019, ME design


title: EXP2_COL05_ROW13


title: pTH11533_HK_8mer_12534

source_name: AT5G25790, HK design

value: TCR


title: EXP2_COL03_ROW37


title: EXP2_COL03_ROW36


title: pTH11551_ME_8mer_12287

source_name: ERF119, ME design


title: EXP2_COL03_ROW39


title: pTH11533_ME_8mer_12355

source_name: AT5G25790, ME design


title: EXP2_COL03_ROW35


title: EXP2_COL03_ROW33


title: EXP2_COL03_ROW27


title: pTH11493_HK_8mer_12212

source_name: TVAG_474020, HK design


title: pTH11493_ME_8mer_12196

source_name: TVAG_474020, ME design


title: EXP2_COL03_ROW31


title: EXP2_COL04_ROW03


title: EXP2_COL03_ROW01


title: pTH11489_HK_8mer_12370

source_name: CBG04010, HK design


title: pTH11531_HK_8mer_12179

source_name: DPBF3, HK design


title: EXP2_COL04_ROW02


title: pTH11492_HK_8mer_12209

source_name: CBG13549, HK design


title: EXP2_COL03_ROW09


title: EXP2_COL02_ROW23


title: EXP2_COL02_ROW25


title: pTH11477_HK_8mer_12368

source_name: CBG20882, HK design


title: pTH11475_HK_8mer_12214

source_name: CBG18054, HK design


title: pTH11477_ME_8mer_12260

source_name: CBG20882, ME design


title: EXP2_COL02_ROW09


title: pTH11475_ME_8mer_12232

source_name: CBG18054, ME design


title: pTH11531_ME_8mer_12163

source_name: DPBF3, ME design


title: EXP2_COL01_ROW40


title: EXP2_COL02_ROW35


title: EXP2_COL02_ROW19


title: pTH11471_ME_8mer_12258

source_name: CBG00029, ME design


title: pTH11492_ME_8mer_12193

source_name: CBG13549, ME design


title: pTH11471_HK_8mer_12366

source_name: CBG00029, HK design


title: EXP2_COL03_ROW25

source_name: single-cell suspension from whole glomerulus, kidney


title: EXP2_COL01_ROW36


title: pTH11469_ME_8mer_12474

source_name: PK07741.1, ME design


title: EXP2_COL01_ROW35


title: EXP2_COL03_ROW14


title: EXP2_COL01_ROW34


title: pTH11469_HK_8mer_12490

source_name: PK07741.1, HK design


title: pTH11464_HK_8mer_11957

source_name: PK10344.1, HK design


title: EXP2_COL01_ROW28


title: pTH11464_ME_8mer_11941

source_name: PK10344.1, ME design


title: pTH11473_ME_8mer_12259

source_name: CBG10466, ME design


title: EXP2_COL01_ROW23


title: pTH11463_HK_8mer_12303

source_name: MLX, HK design


title: pTH11463_ME_8mer_12257

source_name: MLX, ME design


title: pTH11462_ME_8mer_12256

source_name: TIGD6, ME design


title: EXP2_COL01_ROW21


title: pTH11489_ME_8mer_12267

source_name: CBG04010, ME design


title: EXP2_COL01_ROW19


title: pTH11473_HK_8mer_12367

source_name: CBG10466, HK design


title: pTH11462_HK_8mer_12302

source_name: TIGD6, HK design


title: pTH11460_ME_8mer_12255

source_name: CBG26996_CONSTRUCT, ME design


title: pTH11460_HK_8mer_12301

source_name: CBG26996_CONSTRUCT, HK design


title: EXP2_COL01_ROW26


title: pTH11459_ME_8mer_12105

source_name: CBG23697_CONSTRUCT, ME design


title: EXP2_COL01_ROW16


title: EXP2_COL01_ROW12


title: pTH11459_HK_8mer_12249

source_name: CBG23697_CONSTRUCT, HK design


title: EXP2_COL01_ROW09


title: EXP2_COL01_ROW08


title: pTH11458_HK_8mer_12120

source_name: CBG23031, HK design


title: pTH11455_ME_8mer_12101

source_name: CBG07341, ME design


title: pTH11458_ME_8mer_12104

source_name: CBG23031, ME design


title: EXP2_COL01_ROW07

source_name: single-cell suspension from whole glomerulus, kidney


title: EXP2_COL01_ROW32


title: EXP2_COL01_ROW05


title: pTH11455_HK_8mer_12117

source_name: CBG07341, HK design


title: EXP2_COL01_ROW13


title: EXP2_COL01_ROW15


title: EXP1_COL20_ROW40

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL20_ROW35

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP2_COL01_ROW01


title: pTH11454_HK_8mer_12116

source_name: CBG01820, HK design


title: pTH11451_HK_8mer_12213

source_name: BATDEDRAFT_37200, HK design


title: pTH11454_ME_8mer_12100

source_name: CBG01820, ME design


title: pTH11451_ME_8mer_12197

source_name: BATDEDRAFT_37200, ME design


title: EXP1_COL20_ROW39

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP2_COL01_ROW04


title: pTH11449_HK_8mer_12114

source_name: CBG23380, HK design


title: pTH11449_ME_8mer_12098

source_name: CBG23380, ME design


title: EXP1_COL20_ROW34

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11445_ME_8mer_12094

source_name: CBG14292, ME design

value: Forkhead


title: EXP1_COL20_ROW33

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11444_ME_8mer_12093

source_name: CBG10835, ME design


title: EXP1_COL20_ROW25

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL20_ROW21

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11444_HK_8mer_12183

source_name: CBG10835, HK design


title: pTH11445_HK_8mer_12110

source_name: CBG14292, HK design


title: pTH11439_ME_8mer_12252

source_name: TIGD2, ME design


title: EXP1_COL20_ROW14

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11439_HK_8mer_12300

source_name: TIGD2, HK design


title: EXP1_COL20_ROW13

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL20_ROW20

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11436_ME_8mer_12470

source_name: PK07402.1, ME design


title: EXP1_COL20_ROW19

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11435_HK_8mer_12365

source_name: PK07031.1, HK design


title: pTH11436_HK_8mer_12486

source_name: PK07402.1, HK design


title: pTH11435_ME_8mer_12283

source_name: PK07031.1, ME design


title: EXP1_COL20_ROW08

description:
exp1_counts.txt
exp1_spike_counts.txt

source_name: single-cell suspension from whole glomerulus, kidney


title: EXP1_COL20_ROW11

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL20_ROW06

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL20_ROW09

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL20_ROW10

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL20_ROW28

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11434_ME_8mer_12251

source_name: FGRRES_05925, ME design


title: pTH11434_HK_8mer_12299

source_name: FGRRES_05925, HK design


title: pTH11431_ME_8mer_12467

source_name: PK07924.1, ME design


title: pTH11431_HK_8mer_12483

source_name: PK07924.1, HK design


title: EXP1_COL20_ROW12

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL20_ROW02

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11426_ME_8mer_12407

source_name: PK01630.1, ME design


title: EXP1_COL20_ROW03

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11424_ME_8mer_12231

source_name: FGRRES_08930, ME design


title: EXP1_COL20_ROW01

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL19_ROW31

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL19_ROW33

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL19_ROW32

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11426_HK_8mer_12423

source_name: PK01630.1, HK design


title: EXP1_COL19_ROW30

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11424_HK_8mer_12247

source_name: FGRRES_08930, HK design


title: pTH11423_ME_8mer_12406

source_name: PK12011.1, ME design


title: pTH11421_HK_8mer_12420

source_name: PK08602.1, HK design


title: pTH11421_ME_8mer_12404

source_name: PK08602.1, ME design


title: pTH11415_ME_8mer_12230

source_name: CREB3L2, ME design


title: EXP1_COL19_ROW28

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL19_ROW29

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11415_HK_8mer_12246

source_name: CREB3L2, HK design


title: EXP1_COL19_ROW25

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL19_ROW23

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL19_ROW24

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11414_ME_8mer_12229

source_name: FGRRES_13344_M, ME design


title: EXP1_COL19_ROW20

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11414_HK_8mer_12245

source_name: FGRRES_13344_M, HK design


title: pTH11412_HK_8mer_12243

source_name: FGRRES_17512, HK design


title: pTH11412_ME_8mer_12227

source_name: FGRRES_17512, ME design


title: EXP1_COL19_ROW18

description:
exp1_counts.txt
exp1_spike_counts.txt

source_name: single-cell suspension from whole glomerulus, kidney


title: pTH11409_HK_8mer_12240

source_name: FGRRES_07198, HK design


title: EXP1_COL19_ROW12

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL19_ROW14

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11409_ME_8mer_12224

source_name: FGRRES_07198, ME design


title: pTH11380_ME_8mer_12221

source_name: FGRRES_05958, ME design


title: EXP1_COL19_ROW11

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL19_ROW10

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11423_HK_8mer_12422

source_name: PK12011.1, HK design


title: pTH11380_HK_8mer_12237

source_name: FGRRES_05958, HK design


title: pTH11377_ME_8mer_12218

source_name: FGRRES_05233, ME design


title: EXP1_COL19_ROW07

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL19_ROW08

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11377_HK_8mer_12234

source_name: FGRRES_05233, HK design


title: pTH11374_ME_8mer_12511

source_name: PK09702.1, ME design


title: EXP1_COL19_ROW06

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11374_HK_8mer_12527

source_name: PK09702.1, HK design


title: EXP1_COL19_ROW05

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL19_ROW04

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL19_ROW03

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11368_ME_8mer_12506

source_name: PK05923.1, ME design


title: EXP1_COL19_ROW02

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11370_HK_8mer_12524

source_name: PK07858.1, HK design


title: pTH11368_HK_8mer_12522

source_name: PK05923.1, HK design


title: pTH11367_HK_8mer_12521

source_name: PK05732.1, HK design


title: pTH11367_ME_8mer_12505

source_name: PK05732.1, ME design


title: EXP1_COL19_ROW01

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11347_ME_8mer_12036

source_name: PK27683.1, ME design


title: pTH11347_HK_8mer_12052

source_name: PK27683.1, HK design


title: EXP1_COL18_ROW36

description:
exp1_counts.txt
exp1_spike_counts.txt

source_name: single-cell suspension from whole glomerulus, kidney


title: EXP1_COL18_ROW35

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11345_ME_8mer_12035

source_name: PK27404.1_CONSTRUCT, ME design


title: EXP1_COL18_ROW22

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11345_HK_8mer_12051

source_name: PK27404.1_CONSTRUCT, HK design


title: EXP1_COL18_ROW19

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL18_ROW18

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL19_ROW09

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11342_ME_8mer_11936

source_name: PK05432.1, ME design

value: WRKY


title: EXP1_COL18_ROW06

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11338_HK_8mer_12042

source_name: PK00377.1, HK design


title: EXP1_COL18_ROW15

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11342_HK_8mer_11952

source_name: PK05432.1, HK design


title: pTH11370_ME_8mer_12508

source_name: PK07858.1, ME design


title: EXP1_COL18_ROW14

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL18_ROW03

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL18_ROW05

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11338_ME_8mer_12026

source_name: PK00377.1, ME design


title: pTH11336_ME_8mer_11847

source_name: PK11990.1, ME design

value: NAM


title: pTH11336_HK_8mer_11863

source_name: PK11990.1, HK design


title: pTH11332_HK_8mer_11859

source_name: PK20392.1, HK design


title: pTH11332_ME_8mer_11843

source_name: PK20392.1, ME design


title: EXP1_COL18_ROW01

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL17_ROW40

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11330_ME_8mer_11841

source_name: PK13314.1, ME design


title: EXP1_COL18_ROW28

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL17_ROW38

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11331_ME_8mer_11842


title: pTH11331_HK_8mer_11858


title: EXP1_COL17_ROW20

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL17_ROW14

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11324_ME_8mer_11835

source_name: PK14653.1, ME design


title: pTH11328_ME_8mer_11839

source_name: PK26618.1, ME design


title: EXP1_COL17_ROW18

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL17_ROW15

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11328_HK_8mer_11855

source_name: PK26618.1, HK design


title: EXP1_COL17_ROW11

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL17_ROW12

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11324_HK_8mer_11851

source_name: PK14653.1, HK design


title: EXP1_COL17_ROW10

description:
exp1_counts.txt
exp1_spike_counts.txt

source_name: single-cell suspension from whole glomerulus, kidney


title: pTH11304_ME_8mer_11711

source_name: PK26523.1, ME design


title: EXP1_COL17_ROW06

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL17_ROW09

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11305_ME_8mer_11712

source_name: PK28187.1, ME design


title: pTH11302_HK_8mer_11726

source_name: PK27693.1, HK design


title: EXP1_COL16_ROW38

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11330_HK_8mer_11857

source_name: PK13314.1, HK design


title: EXP1_COL17_ROW02

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL16_ROW39

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL16_ROW30

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11305_HK_8mer_11926

source_name: PK28187.1, HK design


title: pTH11302_ME_8mer_11710

source_name: PK27693.1, ME design


title: EXP1_COL17_ROW04

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11299_ME_8mer_11707

source_name: PK21687.1, ME design


title: pTH11300_ME_8mer_11708

source_name: PK24181.1, ME design


title: pTH11304_HK_8mer_11727

source_name: PK26523.1, HK design


title: EXP1_COL16_ROW24

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL16_ROW40

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11300_HK_8mer_11724

source_name: PK24181.1, HK design


title: pTH11298_HK_8mer_11722

source_name: PK20317.1, HK design


title: EXP1_COL16_ROW19

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11299_HK_8mer_11723

source_name: PK21687.1, HK design


title: EXP1_COL16_ROW17

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL16_ROW28

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11298_ME_8mer_11706

source_name: PK20317.1, ME design


title: EXP1_COL16_ROW18

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11283_HK_8mer_11718

source_name: PK27149.1, HK design


title: pTH11280_ME_8mer_11683

source_name: PK24580.1, ME design


title: pTH11283_ME_8mer_11702

source_name: PK27149.1, ME design


title: EXP1_COL16_ROW16

description:
exp1_counts.txt
exp1_spike_counts.txt

source_name: single-cell suspension from whole glomerulus, kidney


title: pTH11276_ME_8mer_11972

source_name: PK20942.1, ME design


title: pTH11276_HK_8mer_11695

source_name: PK20942.1, HK design


title: EXP1_COL16_ROW14

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL16_ROW07

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL16_ROW13

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11274_HK_8mer_11693

source_name: PK20225.1, HK design


title: EXP1_COL16_ROW05

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL16_ROW11

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11273_ME_8mer_11676

source_name: PK19166.1, ME design


title: EXP1_COL16_ROW02

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL15_ROW37

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11272_ME_8mer_11675

source_name: PK18401.1, ME design


title: pTH11272_HK_8mer_11691

source_name: PK18401.1, HK design


title: pTH11271_HK_8mer_11950

source_name: HDX, HK design


title: pTH11271_ME_8mer_11934

source_name: HDX, ME design


title: EXP1_COL16_ROW15

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL15_ROW32

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11270_ME_8mer_11933

source_name: SOHLH2, ME design


title: EXP1_COL15_ROW35

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL15_ROW34

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL15_ROW31

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11280_HK_8mer_11699

source_name: PK24580.1, HK design


title: EXP1_COL15_ROW30

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11274_ME_8mer_11677

source_name: PK20225.1, ME design


title: EXP1_COL16_ROW03

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11267_ME_8mer_11748

source_name: LCORL, ME design


title: EXP1_COL15_ROW26

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11268_ME_8mer_11931

source_name: ZSCAN29, ME design


title: pTH11267_HK_8mer_11764

source_name: LCORL, HK design


title: pTH11266_ME_8mer_11747

source_name: FOXN3, ME design


title: pTH11273_HK_8mer_11692

source_name: PK19166.1, HK design


title: EXP1_COL15_ROW24

description:
exp1_counts.txt
exp1_spike_counts.txt

source_name: single-cell suspension from whole glomerulus, kidney


title: pTH11266_HK_8mer_11763

source_name: FOXN3, HK design


title: EXP1_COL15_ROW22

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL15_ROW25

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11265_ME_8mer_11746

source_name: VEZF1, ME design


title: pTH11265_HK_8mer_11762

source_name: VEZF1, HK design


title: pTH11262_ME_8mer_11672

source_name: PK27085.1, ME design


title: EXP1_COL15_ROW20

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL15_ROW19

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11261_ME_8mer_11671

source_name: PK26376.1, ME design


title: pTH11262_HK_8mer_11688

source_name: PK27085.1, HK design


title: EXP1_COL15_ROW15

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11270_HK_8mer_11949

source_name: SOHLH2, HK design


title: pTH11261_HK_8mer_11687

source_name: PK26376.1, HK design


title: EXP1_COL15_ROW27

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11268_HK_8mer_11947

source_name: ZSCAN29, HK design


title: EXP1_COL15_ROW28

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL15_ROW11

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11260_ME_8mer_11670

source_name: PK26075.1, ME design


title: EXP1_COL15_ROW08

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL15_ROW09

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11259_ME_8mer_11669

source_name: PK25034.1, ME design


title: pTH11259_HK_8mer_11685

source_name: PK25034.1, HK design


title: EXP1_COL15_ROW23

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL15_ROW05

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL15_ROW06

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL15_ROW07

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11258_ME_8mer_11620

source_name: PK25011.1, ME design


title: pTH11255_ME_8mer_11617

source_name: PK21166.1, ME design


title: pTH11260_HK_8mer_11686

source_name: PK26075.1, HK design


title: EXP1_COL14_ROW40

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL15_ROW03

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL15_ROW02

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL14_ROW39

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL15_ROW10

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL14_ROW31

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11252_HK_8mer_11630

source_name: PK19314.1, HK design


title: pTH11252_ME_8mer_11614

source_name: PK19314.1, ME design


title: EXP1_COL14_ROW35

description:
exp1_counts.txt
exp1_spike_counts.txt

source_name: single-cell suspension from whole glomerulus, kidney


title: pTH11253_ME_8mer_11615

source_name: PK20543.1, ME design


title: pTH11253_HK_8mer_11631

source_name: PK20543.1, HK design


title: EXP1_COL14_ROW24

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11249_ME_8mer_11881

source_name: ZZZ3, ME design


title: EXP1_COL14_ROW22

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11241_ME_8mer_11781

source_name: MSANTD3, ME design


title: EXP1_COL14_ROW17

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11241_HK_8mer_11797

source_name: MSANTD3, HK design


title: EXP1_COL14_ROW19

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL14_ROW16

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11249_HK_8mer_11897

source_name: ZZZ3, HK design


title: pTH11233_HK_8mer_11760

source_name: CENPBD1, HK design


title: pTH11258_HK_8mer_11636

source_name: PK25011.1, HK design


title: pTH11233_ME_8mer_11744

source_name: CENPBD1, ME design


title: EXP1_COL14_ROW15

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11255_HK_8mer_11633

source_name: PK21166.1, HK design


title: EXP1_COL14_ROW14

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11227_ME_8mer_11772

source_name: TIGD1, ME design


title: pTH11226_ME_8mer_11771

source_name: DUXA, ME design


title: pTH11227_HK_8mer_11788

source_name: TIGD1, HK design


title: EXP1_COL14_ROW05

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL14_ROW02

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL14_ROW01

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL14_ROW18

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL13_ROW35

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11223_ME_8mer_11738

source_name: ZBTB44, ME design


title: pTH11223_HK_8mer_11754

source_name: ZBTB44, HK design


title: pTH11216_ME_8mer_11715

source_name: CXXC5, ME design

value: zf-CXXC


title: pTH11216_HK_8mer_11731

source_name: CXXC5, HK design


title: EXP1_COL13_ROW33

description:
exp1_counts.txt
exp1_spike_counts.txt

source_name: single-cell suspension from whole glomerulus, kidney


title: EXP1_COL13_ROW32

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11214_ME_8mer_11713

source_name: PLAGL2, ME design


title: pTH11214_HK_8mer_11924

source_name: PLAGL2, HK design


title: EXP1_COL13_ROW29

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL13_ROW26

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL13_ROW21

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11169_ME_8mer_11375

source_name: Phyra84400, ME design


title: EXP1_COL13_ROW22

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11170_ME_8mer_11376

source_name: Phyra76486, ME design


title: EXP1_COL14_ROW09

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11169_HK_8mer_11391

source_name: Phyra84400, HK design


title: EXP1_COL14_ROW08

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11226_HK_8mer_11787

source_name: DUXA, HK design


title: EXP1_COL13_ROW20

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL13_ROW19

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL13_ROW18

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL13_ROW17

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL13_ROW12

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL13_ROW10

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL13_ROW09

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL13_ROW11

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL13_ROW03

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11170_HK_8mer_11442

source_name: Phyra76486, HK design


title: pTH11157_HK_8mer_11330

source_name: CNI01970, HK design


title: pTH11158_HK_8mer_11331

source_name: FGRRES_04288, HK design


title: pTH11155_HK_8mer_11328

source_name: HCBG_05540, HK design


title: EXP1_COL12_ROW36

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11157_ME_8mer_11408

source_name: CNI01970, ME design


title: pTH11155_ME_8mer_11406

source_name: HCBG_05540, ME design


title: EXP1_COL12_ROW27

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL13_ROW24

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11154_HK_8mer_11327


title: pTH11158_ME_8mer_11409

source_name: FGRRES_04288, ME design


title: pTH11154_ME_8mer_11405


title: EXP1_COL12_ROW24

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11147_HK_8mer_11320


title: pTH11147_ME_8mer_11398


title: EXP1_COL12_ROW34

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL12_ROW25

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11167_HK_8mer_11389

source_name: Phyra42912, HK design


title: pTH11168_HK_8mer_11390

source_name: BATDEDRAFT_9451, HK design


title: pTH11167_ME_8mer_11373

source_name: Phyra42912, ME design


title: pTH11146_ME_8mer_11348

source_name: HCBG_00900, ME design


title: EXP1_COL12_ROW23

description:
exp1_counts.txt
exp1_spike_counts.txt

source_name: single-cell suspension from whole glomerulus, kidney


title: EXP1_COL12_ROW22

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11146_HK_8mer_11319

source_name: HCBG_00900, HK design


title: pTH11144_ME_8mer_11346

source_name: rst2, ME design


title: pTH11143_ME_8mer_11397

source_name: prz1, ME design


title: EXP1_COL12_ROW21

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL12_ROW18

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11143_HK_8mer_11316

source_name: prz1, HK design


title: EXP1_COL12_ROW16

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL12_ROW17

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11142_HK_8mer_11315

source_name: ZAT4, HK design


title: pTH11142_ME_8mer_11299

source_name: ZAT4, ME design


title: pTH11140_HK_8mer_11313

source_name: SCHCODRAFT_84749, HK design


title: EXP1_COL12_ROW14

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL12_ROW09

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL12_ROW15

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11138_HK_8mer_11311

source_name: PGTG_04827, HK design


title: pTH11140_ME_8mer_11297

source_name: SCHCODRAFT_84749, ME design


title: EXP1_COL12_ROW05

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL12_ROW07

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL12_ROW13

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10279_ME_8mer_11607

source_name: PK23009.1, ME design


title: pTH10625_ME_8mer_11411

source_name: lin-28, ME design

value: CSD


title: pTH11168_ME_8mer_11374

source_name: BATDEDRAFT_9451, ME design

value: ME


title: pTH10625_HK_8mer_10762

source_name: lin-28, HK design


title: EXP1_COL12_ROW01

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11144_HK_8mer_11317

source_name: rst2, HK design


title: EXP1_COL12_ROW02

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10279_HK_8mer_11623

source_name: PK23009.1, HK design


title: EXP1_COL12_ROW04

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH11138_ME_8mer_11426

source_name: PGTG_04827, ME design


title: pTH10273_HK_8mer_11601

source_name: PK18474.1, HK design


title: EXP1_COL11_ROW34

description:
exp1_counts.txt
exp1_spike_counts.txt

source_name: single-cell suspension from whole glomerulus, kidney


title: EXP1_COL11_ROW37

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL12_ROW19

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10272_ME_8mer_11584

source_name: PK18009.1, ME design


title: EXP1_COL11_ROW28

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10272_HK_8mer_11600

source_name: PK18009.1, HK design


title: pTH10268_HK_8mer_11596

source_name: PK15505.1, HK design


title: EXP1_COL11_ROW39

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL11_ROW24

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL11_ROW22

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL11_ROW21

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10264_ME_8mer_11576

source_name: PK10955.1, ME design


title: pTH10268_ME_8mer_11580

source_name: PK15505.1, ME design


title: EXP1_COL11_ROW20

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10261_ME_8mer_11410

source_name: FGRRES_10470, ME design


title: pTH10264_HK_8mer_11592

source_name: PK10955.1, HK design


title: EXP1_COL11_ROW12

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL11_ROW10

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10260_HK_8mer_11211

source_name: FGRRES_01877, HK design


title: pTH10273_ME_8mer_11585

source_name: PK18474.1, ME design


title: pTH10261_HK_8mer_11360

source_name: FGRRES_10470, HK design


title: EXP1_COL11_ROW13

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10260_ME_8mer_11195

source_name: FGRRES_01877, ME design


title: EXP1_COL11_ROW08

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL11_ROW07

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL11_ROW06

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10258_HK_8mer_11209

source_name: rsv2, HK design


title: pTH10258_ME_8mer_11193

source_name: rsv2, ME design


title: pTH10254_ME_8mer_11189

source_name: NCU09576, ME design


title: EXP1_COL11_ROW05

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL11_ROW04

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10254_HK_8mer_11205

source_name: NCU09576, HK design


title: pTH10246_ME_8mer_11134

source_name: ZAT1, ME design


title: EXP1_COL10_ROW40

description:
exp1_counts.txt
exp1_spike_counts.txt

source_name: single-cell suspension from whole glomerulus, kidney

value: Control


title: EXP1_COL10_ROW31

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10246_HK_8mer_11150

source_name: ZAT1, HK design

value: HK


title: pTH10222_ME_8mer_10725

source_name: RO3G_06280, ME design


title: EXP1_COL10_ROW21

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL10_ROW08

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL10_ROW16

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10205_HK_8mer_9468

source_name: NCU06487, HK design


title: EXP1_COL10_ROW24

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10205_ME_8mer_9452

source_name: NCU06487, ME design


title: EXP1_COL10_ROW07

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10193_ME_8mer_11555

source_name: PK20442.1, ME design


title: pTH10194_ME_8mer_11556

source_name: PK23763.1, ME design


title: pTH10196_HK_8mer_11590

source_name: PK27213.1, HK design


title: EXP1_COL10_ROW03

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10193_HK_8mer_11571

source_name: PK20442.1, HK design


title: pTH10196_ME_8mer_11574

source_name: PK27213.1, ME design


title: pTH10194_HK_8mer_11572

source_name: PK23763.1, HK design


title: EXP1_COL09_ROW36

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL09_ROW33

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL09_ROW37

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10187_ME_8mer_11549

source_name: PK16335.1, ME design


title: EXP1_COL10_ROW05

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL09_ROW34

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10187_HK_8mer_11565

source_name: PK16335.1, HK design


title: pTH10186_HK_8mer_11564

source_name: PK15337.1, HK design


title: EXP1_COL09_ROW31

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10186_ME_8mer_11548

source_name: PK15337.1, ME design


title: EXP1_COL09_ROW32

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL09_ROW30

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10180_ME_8mer_12064

source_name: PK12240.1, ME design


title: pTH10222_HK_8mer_10741

source_name: RO3G_06280, HK design


title: EXP1_COL09_ROW29

description:
exp1_counts.txt
exp1_spike_counts.txt

source_name: single-cell suspension from whole glomerulus, kidney


title: EXP1_COL09_ROW19

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL09_ROW27

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10176_ME_8mer_11650

source_name: PK09091.1, ME design


title: pTH10180_HK_8mer_11558

source_name: PK12240.1, HK design


title: EXP1_COL09_ROW07

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL09_ROW02

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10165_ME_8mer_11639

source_name: PK03929.1, ME design


title: pTH10165_HK_8mer_11655

source_name: PK03929.1, HK design


title: pTH10175_ME_8mer_12063

source_name: PK09021.1, ME design


title: pTH10163_HK_8mer_11653

source_name: PK03025.1, HK design


title: EXP1_COL09_ROW05

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10175_HK_8mer_11665

source_name: PK09021.1, HK design


title: pTH10161_ME_8mer_11521

source_name: PK01261.1, ME design


title: pTH10161_HK_8mer_11537

source_name: PK01261.1, HK design


title: EXP1_COL08_ROW37

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL08_ROW36

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL09_ROW06

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL08_ROW31

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL08_ROW30

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10160_ME_8mer_11520

source_name: PK01144.1, ME design


title: pTH10163_ME_8mer_11637

source_name: PK03025.1, ME design


title: EXP1_COL08_ROW35

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10160_HK_8mer_11536

source_name: PK01144.1, HK design


title: EXP1_COL09_ROW01

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL08_ROW39

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10176_HK_8mer_11666

source_name: PK09091.1, HK design


title: pTH10158_ME_8mer_11518

source_name: PK27109.1, ME design


title: EXP1_COL08_ROW27

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL09_ROW14

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL08_ROW24

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL08_ROW19

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL08_ROW20

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10156_ME_8mer_11516

source_name: PK25870.1, ME design


title: pTH10156_HK_8mer_11532

source_name: PK25870.1, HK design


title: pTH10153_HK_8mer_11529

source_name: PK24978.1, HK design


title: pTH10153_ME_8mer_11513

source_name: PK24978.1, ME design


title: pTH10152_ME_8mer_11512

source_name: PK24205.1, ME design


title: EXP1_COL08_ROW11

description:
exp1_counts.txt
exp1_spike_counts.txt

source_name: single-cell suspension from whole glomerulus, kidney


title: EXP1_COL08_ROW16

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL08_ROW12

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10152_HK_8mer_11528

source_name: PK24205.1, HK design


title: pTH10151_HK_8mer_11527

source_name: PK23964.1, HK design


title: EXP1_COL08_ROW10

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10151_ME_8mer_11511

source_name: PK23964.1, ME design


title: pTH10144_HK_8mer_11472

source_name: PK20555.1, HK design


title: pTH10143_ME_8mer_11455

source_name: PK20397.1, ME design

value: HLH


title: EXP1_COL08_ROW08

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10144_ME_8mer_11456

source_name: PK20555.1, ME design


title: EXP1_COL08_ROW07

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL08_ROW04

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL08_ROW06

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10158_HK_8mer_11534

source_name: PK27109.1, HK design


title: pTH10142_ME_8mer_11454

source_name: PK19717.1, ME design


title: pTH10143_HK_8mer_11471

source_name: PK20397.1, HK design


title: pTH10142_HK_8mer_11470

source_name: PK19717.1, HK design


title: EXP1_COL08_ROW02

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL07_ROW38

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10140_ME_8mer_11452

source_name: PK19621.1, ME design


title: pTH10138_ME_8mer_11450

source_name: PK19363.1, ME design


title: pTH10140_HK_8mer_11468

source_name: PK19621.1, HK design


title: pTH10138_HK_8mer_11466

source_name: PK19363.1, HK design


title: EXP1_COL07_ROW35

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10133_ME_8mer_11445

source_name: PK17878.1, ME design


title: pTH10133_HK_8mer_11461

source_name: PK17878.1, HK design


title: EXP1_COL07_ROW34

description:
exp1_counts.txt
exp1_spike_counts.txt

source_name: single-cell suspension from whole glomerulus, kidney


title: pTH10129_ME_8mer_11489

source_name: PK16340.1, ME design


title: pTH10129_HK_8mer_11505

source_name: PK16340.1, HK design


title: pTH10125_ME_8mer_11485

source_name: PK15172.1, ME design


title: pTH10125_HK_8mer_11501

source_name: PK15172.1, HK design


title: EXP1_COL07_ROW32

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL07_ROW33

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL07_ROW31

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10123_ME_8mer_11483

source_name: PK14080.1, ME design


title: pTH10123_HK_8mer_11499

source_name: PK14080.1, HK design


title: EXP1_COL07_ROW30

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10121_ME_8mer_12061

source_name: PK12736.1, ME design


title: pTH10121_HK_8mer_11983

source_name: PK12736.1, HK design


title: BXD103 High Fat Diet_scWAT

type: RNA

city: Lausanne

first: Johan

last: Auwerx

address: Station 15

department: SV IBI NCEM

institute: École Polytechnique Fédérale de Lausanne

description: Gene expression data

hyb_protocol: Labeled cDNA was hybridized according to Affymetrix protocols. Approximately 16hr incubation and then washed and stained according to Affymetrix protocols on Affymetrix 450 Fluidics Station.

scan_protocol: Arrays were scanned on Affymetrix GSC3000 7G Scanner

data_processing: Samples were analyzed using Affymetrix Expression Console using RMA-sketch

label: biotin

source_name: Mouse subcutaneous white adipose tissue

label_protocol: 100ng of total RNA was amplified using Ambion® WT Expression Kit from life technologies (Part Number 4411974) according to manufactures protocols, then 5500ng cDNA was fragmented and labeled using Affymetrix WT terminal labeling kit (part number 900671) according to manufactures protocols.

growth_protocol: Mice are housed 5 per cage in IVC GM500 cages (Tecniplast, 500 cm2) according to the Swiss cantonal rules.

extract_protocol: Trizol extraction of total RNA was performed on all individuals -- approximately 5 per cohort, for roughly 400 animals in all. Sample concentrations were then measured by Nanodrop, and then all individuals from a cohort were evenly pooled together. These cohort pools were then cleaned up with a Qiagen RNEasy kit prior to preparation for microarray.

treatment_protocol: Male mice in chow diet cohorts were Harlan 2018 throughout their lifetime until sacrifice at 29 weeks. Male mice in the high fat diet cohorts were fed Harlan 06414 from week 8 until sacrifice at 29 weeks of age. Food was ad libitum except prior to sacrifice, when all animals were fasted overnight (6pm to 9am).

tag: tissue

value: subcutaneous white adipose tissue

tag: diet

value: High fat

tag: Sex

value: male

value: 29 weeks


title: C57BL/6J High Fat Diet_scWAT

description: Gene expression data


title: pTH10114_HK_8mer_11248

source_name: PK09653.1, HK design


title: DBA/2J Chow Diet_scWAT

description: Gene expression data

value: Chow


title: DBA/2J High Fat Diet_scWAT

description: Gene expression data


title: C57BL/6J Chow Diet_scWAT

description: Gene expression data


title: BXD103 Chow Diet_scWAT

description: Gene expression data


title: EXP1_COL07_ROW27

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL07_ROW25

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10114_ME_8mer_11232

source_name: PK09653.1, ME design


title: EXP1_COL07_ROW29

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL07_ROW21

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL07_ROW20

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10099_ME_8mer_11056

source_name: PK05128.1, ME design


title: pTH10099_HK_8mer_11072

source_name: PK05128.1, HK design


title: pTH10104_ME_8mer_11061

source_name: PK06517.1, ME design


title: pTH10104_HK_8mer_11362

source_name: PK06517.1, HK design


title: pTH10100_HK_8mer_11147

source_name: PK05451.1, HK design


title: pTH10101_HK_8mer_11074

source_name: PK05671.1, HK design

value: zf-Dof


title: pTH10100_ME_8mer_11131

source_name: PK05451.1, ME design


title: pTH10101_ME_8mer_11058

source_name: PK05671.1, ME design


title: EXP1_COL07_ROW18

description:
exp1_counts.txt
exp1_spike_counts.txt

source_name: single-cell suspension from whole glomerulus, kidney

value: Postnatal week 10


title: pTH10095_HK_8mer_11051

source_name: PK03576.1, HK design


title: pTH10098_ME_8mer_11038

source_name: PK04914.1, ME design


title: pTH10098_HK_8mer_11361

source_name: PK04914.1, HK design


title: EXP1_COL07_ROW19

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10093_HK_8mer_11049

source_name: PK02330.1, HK design


title: EXP1_COL07_ROW16

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pTH10095_ME_8mer_11035

source_name: PK03576.1, ME design


title: BXD101 Chow Diet_scWAT

description: Gene expression data

source_name: Mouse subcutaneous white adipose tissue


title: BXD99 Chow Diet_scWAT

description: Gene expression data


title: BXD100 Chow Diet_scWAT

description: Gene expression data


title: BXD98 High Fat Diet_scWAT

description: Gene expression data


title: pTH10020_ME_8mer_8889

source_name: fos-1, ME design


title: EXP1_COL07_ROW15

description:
exp1_counts.txt
exp1_spike_counts.txt


title: BXD99 High Fat Diet_scWAT

description: Gene expression data


title: pEX0428_HK_8mer_10953

source_name: ANIA_10597, HK design


title: BXD96 Chow Diet_scWAT

description: Gene expression data


title: BXD101 High Fat Diet_scWAT

description: Gene expression data


title: BXD97 High Fat Diet_scWAT

description: Gene expression data


title: BXD97 Chow Diet_scWAT

description: Gene expression data


title: BXD100 High Fat Diet_scWAT

description: Gene expression data


title: BXD98 Chow Diet_scWAT

description: Gene expression data


title: pTH10093_ME_8mer_11033

source_name: PK02330.1, ME design


title: pEX0427_ME_8mer_10920

source_name: ANIA_10600, ME design


title: pTH10085_ME_8mer_11420

source_name: PK00315.1, ME design


title: pEX0427_HK_8mer_10952

source_name: ANIA_10600, HK design


title: pEX0428_ME_8mer_10921

source_name: ANIA_10597, ME design


title: pTH10085_HK_8mer_11436

source_name: PK00315.1, HK design


title: pEX0430_ME_8mer_10923

source_name: ANIA_04785, ME design


title: pTH10020_HK_8mer_11923

source_name: fos-1, HK design


title: EXP1_COL07_ROW14

description:
exp1_counts.txt
exp1_spike_counts.txt

source_name: single-cell suspension from whole glomerulus, kidney


title: pEX0430_HK_8mer_10955

source_name: ANIA_04785, HK design


title: EXP1_COL07_ROW13

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL07_ROW11

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pEX0423_HK_8mer_10948

source_name: ANIA_03637, HK design


title: pEX0424_HK_8mer_10949

source_name: ANIA_03502, HK design


title: BXD90 Chow Diet_scWAT

description: Gene expression data


title: EXP1_COL07_ROW09

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pEX0426_ME_8mer_10919

source_name: ANIA_04837, ME design


title: BXD89 High Fat Diet_scWAT

description: Gene expression data


title: EXP1_COL07_ROW08

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pEX0420_HK_8mer_10945

source_name: ANIA_03769, HK design


title: pEX0426_HK_8mer_10951

source_name: ANIA_04837, HK design


title: BXD90 High Fat Diet_scWAT

description: Gene expression data

source_name: Mouse subcutaneous white adipose tissue


title: pEX0424_ME_8mer_10917

source_name: ANIA_03502, ME design


title: BXD95 High Fat Diet_scWAT

description: Gene expression data


title: BXD92a Chow Diet_scWAT

description: Gene expression data


title: BXD89 Chow Diet_scWAT

description: Gene expression data


title: pEX0419_HK_8mer_10944

source_name: ANIA_10483, HK design


title: BXD95 Chow Diet_scWAT

description: Gene expression data


title: EXP1_COL07_ROW05

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pEX0420_ME_8mer_10913

source_name: ANIA_03769, ME design


title: pEX0419_ME_8mer_10912

source_name: ANIA_10483, ME design


title: EXP1_COL07_ROW04

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL06_ROW39

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL06_ROW40

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pEX0423_ME_8mer_11004

source_name: ANIA_03637, ME design


title: EXP1_COL07_ROW06

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL06_ROW38

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pEX0415_ME_8mer_10892

source_name: ANIA_04118, ME design


title: pEX0418_HK_8mer_10911

source_name: ANIA_10491, HK design


title: pEX0417_HK_8mer_10910

source_name: ANIA_03986, HK design


title: pEX0413_ME_8mer_10890

source_name: ANIA_08161, ME design


title: pEX0413_HK_8mer_10906

source_name: ANIA_08161, HK design


title: EXP1_COL06_ROW35

description:
exp1_counts.txt
exp1_spike_counts.txt

source_name: single-cell suspension from whole glomerulus, kidney


title: BXD87 Chow Diet_scWAT

description: Gene expression data


title: pEX0410_HK_8mer_10903

source_name: ANIA_08125, HK design


title: EXP1_COL06_ROW33

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pEX0418_ME_8mer_10895

source_name: ANIA_10491, ME design


title: pEX0400_ME_8mer_10859

source_name: ANIA_00033, ME design


title: BXD85 High Fat Diet_scWAT

description: Gene expression data


title: EXP1_COL06_ROW30

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pEX0417_ME_8mer_10894

source_name: ANIA_03986, ME design


title: pEX0400_HK_8mer_10875

source_name: ANIA_00033, HK design


title: pEX0399_ME_8mer_10858

source_name: nirA, ME design


title: pEX0415_HK_8mer_10908

source_name: ANIA_04118, HK design


title: pEX0410_ME_8mer_10887

source_name: ANIA_08125, ME design


title: EXP1_COL06_ROW25

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pEX0399_HK_8mer_10874

source_name: nirA, HK design


title: EXP1_COL06_ROW31

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL06_ROW24

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pEX0393_ME_8mer_10852

source_name: ANIA_10083, ME design


title: pEX0393_HK_8mer_10868

source_name: ANIA_10083, HK design


title: pEX0397_ME_8mer_10927

source_name: ANIA_00202, ME design


title: pEX0397_HK_8mer_10872

source_name: ANIA_00202, HK design


title: pEX0390_ME_8mer_10849

source_name: ANIA_10120, ME design


title: BXD85 Chow Diet_scWAT

description: Gene expression data

source_name: Mouse subcutaneous white adipose tissue


title: BXD87 High Fat Diet_scWAT

description: Gene expression data


title: pEX0390_HK_8mer_10865

source_name: ANIA_10120, HK design


title: pEX0389_ME_8mer_10848

source_name: ANIA_00835, ME design


title: EXP1_COL06_ROW23

description:
exp1_counts.txt
exp1_spike_counts.txt

source_name: single-cell suspension from whole glomerulus, kidney


title: BXD84 Chow Diet_scWAT

description: Gene expression data


title: BXD84 High Fat Diet_scWAT

description: Gene expression data


title: BXD81 High Fat Diet_scWAT

description: Gene expression data


title: BXD83 Chow Diet_scWAT

description: Gene expression data


title: BXD83 High Fat Diet_scWAT

description: Gene expression data


title: BXD80 High Fat Diet_scWAT

description: Gene expression data


title: BXD81 Chow Diet_scWAT

description: Gene expression data


title: EXP1_COL06_ROW29

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pEX0389_HK_8mer_10864

source_name: ANIA_00835, HK design


title: pEX0388_ME_8mer_10799

source_name: ANIA_00885, ME design

value: Homeobox


title: pEX0388_HK_8mer_10815

source_name: ANIA_00885, HK design


title: pEX0387_ME_8mer_10798

source_name: ANIA_00928, ME design


title: pEX0387_HK_8mer_10814

source_name: ANIA_00928, HK design


title: EXP1_COL06_ROW14

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL06_ROW15

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pEX0386_HK_8mer_10813

source_name: ANIA_01077, HK design


title: EXP1_COL06_ROW22

description:
exp1_counts.txt
exp1_spike_counts.txt


title: pEX0385_ME_8mer_10796

source_name: ANIA_01298, ME design


title: pEX0386_ME_8mer_10797

source_name: ANIA_01077, ME design


title: EXP1_COL06_ROW13

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL06_ROW17

description:
exp1_counts.txt
exp1_spike_counts.txt


title: EXP1_COL06_ROW12

description:
exp1_counts.txt
exp1_spike_counts.txt