title: H460-RUNX3MT-Tam-16

city: Cheongju

first: Jung-Won

address: 1, Chungdae-ro

department: Department of Biochemistry, School of Medicine

institute: Institute for Tumor Research

data_processing:
Illumina NextSeq 500 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence then mapped to hg19 whole genome using STAR (version 2.5.0) with parameters -outFilterIntronMotifs RemoveNoncanonicalUnannotated.
Fragments Per Kilobase of exon per Megabase of library size (FPKM) were calculated by Cufflinks packages (version 2.2.1).
Differentially expressed genes were detected using Cuffdiff (version 2.2.1) against Ensemble genes.
Genome_build: human reference genome hg19
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample.

source_name: large cell lung cancer

growth_protocol: H460 stable cell lines were maintained in RPMI 1640 medium (Gibco BRL) supplemented with 10% fetal bovine serum (Gibco BRL) and 1% penicillin/streptomycin (Invitrogen). All cell lines were incubated at 37°C with 5% CO2.

extract_protocol: Isolated total RNA was processed for preparation of an RNA-seq library using the Illumina TruSeq Stranded mRNA Sample Preparation kit (Illumina, San Diego, CA, USA). Quality and size of libraries were assessed using the Agilent 2100 Bioanalyzer DNA kit (Agilent, Santa Clara, CA, USA). All libraries were quantified by qPCR using a CFX96 Real Time System (Bio-Rad, Hercules, CA, USA) and sequenced on NextSeq500 sequencers (Illumina).

treatment_protocol: H460, H460-ERT2-RUNX3 WT, and H460-ERT2-RUNX3-MT(K94/171R) cells were serum-starved for 24 hr, and then stimulated with 10% serum or 10% serum + 1 uM 4-OHT for 0, 8, or 16 hr.

value: NCI-H460 cell line

value: Tamoxifen-driven stable cell line

value: lung:pleural effusion

tag: passages

value: 11 Cell passage

tag: genotype

value: ERT2-RUNX3 MT insertion

tag: treatment

value: TAM

tag: time


title: H460-RUNX3MT-Tam-0h

status: Public on Mar 13 2019

type: SRA

last: Lee

country: South Korea

library_source: transcriptomic

molecule: total RNA

organism: Homo sapiens

tag: cell line

tag: cell type

tag: tissue

value: control


title: H460-RUNX3MT-Tam-8h


title: Sep09_1_1_DAPIposvsneg_(1)

status: Public on Mar 14 2019

type: RNA

city: Gosselies

first: Raoul

last: Germano

address: 12, rue des Profs. Jeener et Brachet

department: Institute of Molecular Biology and Medicine

country: Belgium

institute: Free University of Brussels

description: Biological replicate 1 of 2, 3dpf DAPI positive ECs versus DAPI negative ECs

hyb_protocol: Scanned on an Agilent G2565AA scanner

scan_protocol: Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1 & 10.10.1.1 for arrays Sep09 and Sep10 respectively).

data_processing: Agilent Feature Extraction Software ((version 10.7.3.1 & 10.10.1.1 for arrays of Sep09 and Sep10 respectively) was used for background subtraction and linear Lowess normalization.

organism: Danio rerio

source_name: zebrafish larvae

label_protocol: Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential

growth_protocol: Zebrafish were maintained and handled at standard conditions (Westerfield 2007), 3dpf corresponds to larvae at 72 hours post fertilization grown at 28dgr, transgenic line used is Tg(kdrl:GFP), mutant strain is gpr124s984 (Vanhollebeke et al_elife_2015)

extract_protocol: 50ng of total RNA was amplified and labeled using Agilent Low Input Quick Amp Labeling Kit

treatment_protocol: Total RNA extracted using Trizol following manufacturer's instructions

tag: strain/genotype

tag: developmental stage

value: DAPI positive ECs

value: DAPI negative ECs


title: Sample_CON_GM_5

city: Bonn

first: Joachim

last: Schultze

state: NRW

address: Carl-Troll-Strasse 31

department: Genomics and Immunoregulation

country: Germany

institute: LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)

data_processing:
Illumina Casava v1.8 software used for basecalling.
Sequenced reads were demultiplexed, then aligned with HiSat2 (v2.0.11) against the mm10 reference genome
Gene and transcript information was extracted with Partek Genomics Suite (v6.6). Annotated data was normalized using statistical software R (v3.3.1) including DESeq2 package.Genes were defined as expressed with normalized RNA-seq values per group ≥ 10.
Processed DeSeq2 normalized RNA-seq tag counts annotated to the hg19 reference genome
Genome_build: hg19

source_name: microglia from occipital cortex

growth_protocol: Corpus callosum and occipital cortex tissue were dissected from control or MS donors that came to autopsy at The Netherlands Brain Bank (NBB) and stored in Hibernate A medium (Invitrogen)

extract_protocol:
Acute isolated microglia were lysed in 1 ml TRisure (Bioline) and total RNA was extracted by collecting aqueous phase after adding chloroform, followed by glycogen precipitation.
RNA-seq library was constructed according to manufacturers' protocol with NEBNext Ultra Directional RNA Library Prep Kit for Illumina

treatment_protocol: Microglia were isolated from post-mortem tissue, using enzymatic dissociation, followed by density gradient centrifugation and magnetic-activated cell sorting using magnetic anti-CD11b beads (Miltenyi)

value: brain

tag: gender

value: f

tag: age

tag: patient


title: H460-RUNX3WT-Tam-0h

description: H460-Tam and H460-RUNX3WT-Tam-genes.fpkm.txt

value: ERT2-RUNX3 WT insertion


title: Sample_CON_GM_4

value: m


title: H460-Tam-0h

value: RUNX3 inactivation


title: Sample_CON_GM_1


title: Sample_CON_GM_2


title: H460-Tam-16h


title: Sample_CON_WM_9

source_name: microglia from corpus callosum


title: Sample_CON_WM_8


title: Sample_CON_WM_6


title: Sample_CON_WM_5


title: Sample_CON_WM_3


title: Sample_CON_WM_1


title: Sample_CON_WM_11


title: Sample_CON_WM_10


title: Sample_CON_WM_2


title: H460-RUNX3WT-Tam-16h


title: Sample_MS_GM_5


title: Sample_MS_GM_4


title: Sample_MS_GM_1


title: Sample_MS_GM_3


title: Sample_MS_WM_9


title: Sample_MS_WM_8


title: H460-Tam-8h


title: H460-RUNX3WT-Tam-8h


title: Sample_CON_GM_3


title: Sample_MS_WM_5


title: Sample_MS_WM_6


title: Sample_MS_WM_7


title: Sample_MS_WM_3


title: Sample_MS_WM_2


title: Sample_MS_WM_11


title: Sample_MS_WM_10


title: Sample_CON_WM_4


title: Sample_MS_WM_1


title: Sample_MS_GM_6


title: RPMI8226_IKZF1

city: Atlanta

first: Benjamin

middle: G

last: Barwick

state: GA

address: 1365 Clifton Rd. NE WCI-C 4th Floor Benches 36-37

department: Hematology and Medical Oncology

country: USA

institute: Emory University

description:
all peaks: RPMI8226_IKZF1_peaks.bed
Super enhancer regions: RPMI8226_IKZF1_peaks.stitch.15000.SE.bed

library_source: genomic

data_processing:
FASTQ files were quality and adapter trimmed using Trim Galore! / Cutadapt (v0.4.2)
ChIP-seq FASTQ files were mapped to the same GRCh37 genome using bowtie 2 (v2.2.6)
IKZF1 enriched regions were determined using MACS2 (v2.1.0.20151222) using default parameters and a q-value of 0.01
Fragment size was estimated using the R package 'chipseq' (v1.28.0) and reads were extended to the estimated fragment size for visualization using the R package 'rtracklayer' (v1.38.3)
Super-enhancer analysis was performed using custom R code in a manner analogous to that done previously (Lovén, J. et al. Cell 153, 320–334 (2013)). Briefly, this involved stitching together enriched regions that were within 15 kb of each other and did not overlap a promoter region defined as 2.5 kb upstream of the TSS for GRCh37.74 defined promoters. Regions were then ranked by occupancy measured as reads per million (RPM) and regions that were past the inflection point were considered super-enhancers.
Genome_build: GRCh37.74
Supplementary_files_format_and_content: bed files of regions enriched for IKZF1 both individually and stitched together as described in the data processing steps

molecule: genomic DNA

source_name: RPMI8226 myeloma cell line

growth_protocol: Cells were cultured at a density of 0.2-1.0 x 106 cells per mL in RPMI 1640 (Corning: 14-030-CV) with 10% FBS, 1% Pen-strep (Corning: 30-002-CI), 1% L-glutamine (Corning: 25005CI), and 1% HEPES (Corning: 25-060-CI).  Cell lines were validated using sequencing and phenotypic characterization.

extract_protocol:
IKZF1 ChIP-seq was performed using 20 x 106 cells, which were cross-linked for 20 min at room temperature with a final concentration of 1% formaldehyde solution (5mM Hepes - KOH pH 7.5; 10mM NaCl; 100 M EDTA pH 8; 5 M EGTA pH 8; 1% formaldehyde) followed by a 5 min quench with 125 mM glycine. Cross-linked cells were washed twice in PBS. 100ul of Dynal protein G magnetic beads (Sigma) were washed 3X for 5 min at 4 C in 0.5% BSA in PBS prior to blocking for >5 hrs with 0.5% BSA in PBS. Magnetic beads were bound with 10  mg of IKZF1 antibody (GeneTex, GTX129438). Cross-linked cells were lysed with lysis buffer 1 (50 mM HEPES pH 7.3; 140 mM NaCl; 1 mM EDTA; 10% glycerol; 0.5% NP-40; 0.25% Triton X-100; protease inhibitors) and resuspended in sonication buffer (20 mM Tris-HCl pH 8.0, 150 mM NaCl, 2 mM EDTA pH 8.0, 0.1% SDS, 1% Triton X-100) prior to sonication on a E210 (Covaris). Sonicated lysates were incubated overnight at 4C with Dynal magnetic beads bound with antibody. Beads were washed two times with sonication buffer, one time with sonication buffer with 500 mM NaCl, one time with LiCl wash buffer (10 mM Tris pH 8; 1 mM EDTA; 250 mM LiCl; 1% NP-40) and one time with TE + 50 mM NaCl. DNA was eluted in elution buffer (50 mM Tris-HCl pH 8; 10 mM EDTA; 1% SDS) at 65C. Cross-links were reversed overnight at 65C and RNA and protein were digested using first RNase A and then Proteinase K at 37C. DNA was purified with phenol chloroform extraction and ethanol precipitated prior to library preparation
Rubicon ThruPLEX DNA-seq (Rubicon Genomics).
Libraries were sequenced to a depth of at least 50 x 106 reads on an Ion Proton (Thermo Fisher) sequencer.

tag: antibody

value: RPMI8226 myeloma cell line


title: MM1S_Input

source_name: MM1S myeloma cell line

value: Input

value: MM1S myeloma cell line


title: Obese mouse 27 ILC1

city: London

first: Victoria

last: Male

state: Not Applicable (Outside of US and Canada)

address: Metabolism, Digestion and Reproduction, Imperial College London, Chelsea and Westminster Hospital Campus, 369 Fulham Road

country: United Kingdom

institute: Imperial College London

data_processing:
BaseCalling was performed using Illumina RTA (v2.4.11)
Fastq was demultiplexed and built using Illumina bcl2fastq (v2.19)
Fastq was then adaptor and quality trimmed using Trimmomatic (v0.36.5)
Processed fastq was aligned to the genome (GTF guided mm10 from Illumina iGenomes) using RNA-STAR (v2.5.2b)
Counts were generated with FeatureCounts (v1.6.0.2)
Normalization of counts and differential expression analysis was carried out using SARtoold (v.1.3.2.0)
Genome_build: UCSC mm10
Supplementary_files_format_and_content: Liver_NK_lean_obese_alldata.txt is a matrix file containing reads and normalised reads for all alignments

organism: Mus musculus

source_name: Intrahepatic leukocytes

growth_protocol: Mice were weaned onto standard RM1 diet or onto a highly palatable obesogenic diet (824018 – ‘45% AFE fat) supplemented with condensed milk. Mice were kept on their respective diets for 12 weeks.

extract_protocol:
Total RNA was extracted from sorted cells using an RNeasy Micro kit (Qiagen)
Libraries were prepared from 2 ng of total RNA using the NEBNext low input kit (New England Biolabs). Libraries were assessed for correct size distribution on the Agilent 2200 TapeStation and quantified by Qubit DNA High Sensitivity assay (Thermo) before being pooled at an equimolar concentration.

treatment_protocol: Mice were killed by direct cervical dislocation, with death confirmed by cessation of circulation. Livers were dissected out and intrahepatic leukocytes were isolated as described in doi: 10.12688/wellcomeopenres.11741.2: 28815219. NK cells (Lin- NK1.1+ CD49a- CD49b+) and ILC1 (Lin- NK1.1+ CD49a+ CD49b-) were sorted.

tag: strain

value: Sorted liver ILC1

tag: diet


title: MM1S_IKZF1_2

description:
all peaks: MM1S_IKZF1_2_peaks.bed
Super enhancer regions: MM1S_IKZF1_2_peaks.stitch.15000.SE.bed


title: ARP1_IKZF1

description:
all peaks: ARP1_IKZF1_peaks.bed
Super enhancer regions: ARP1_IKZF1_peaks.stitch.15000.SE.bed

source_name: ARP1 myeloma cell line

value: ARP1 myeloma cell line


title: Obese mouse 27 cNK

value: Sorted liver NK cells


title: Obese mouse 25 ILC1


title: Sample_CON_WM_7


title: Obese mouse 25 cNK


title: Lean mouse 27 ILC1

value: RM1 expanded diet


title: Lean mouse 26 ILC1


title: Lean mouse 27 cNK


title: Lean mouse 25 cNK


title: Lean mouse 26 cNK


title: Obese mouse 26 cNK


title: RPMI8226_Input


title: KL_siMYB_2

city: Freiburg

first: Pierre

middle: Daniel

last: Cauchy

state: Baden-Württemberg

address: Breisacher Str. 115

department: Zentrum für Translationale Zellforschung

institute: University Medical Center Freiburg

data_processing:
base calling: HiSeq Control Software 2.2.58 / NextSeq Control Software 2.1.2
alignment:tophat2 (2.0.14)
transcript assembly: cufflinks (2.2.1)
FPKM generation: cufflinks (2.2.1)
coverage generation: bedtools genomecov (2.19.0)
Genome_build: mm9
Supplementary_files_format_and_content: bedGraph format

source_name: mouse KL leukemia cell line

growth_protocol: Cells were cultured in RPMI medium supplemented with 10% fetal bovine serum, 50U/ml penicillin, 50μg/ml streptomycin, 2mM L-glutamine. FMH9 cultures were supplemented with 50ng/ml stem cell factor, 5ng/ml granulocyte-macrophage colony-stimulating factor, 5ng/ml interleukin-3, and 5ng interleukin-6, while KL cells (GV and JF, unpublished) and LL cells required 2ng/ml interleukin-3. All cytokines were purchased from Peprotech EC (London, UK).

extract_protocol:
RNA was extracted using RNeasy mini kit (QIAGEN)
Libraries were prepared via the Illumina TruSeq® Stranded mRNA library preparation protocol according to manufacturer's instructions

treatment_protocol: In total, 5 x106 FMH9, KL, or LL cells were electroporated with 300mM of Myb siRNA (s70212, Ambion, Life Technologies) or a scrambled negative control siRNA (4390843 Silencer Select Negative Control #1 – Life Technologies). Using an Amaxa 4D-nucleofector with solution SF Cell Line (V4XC-2024, Lonza) and program EO-100 for FMH9 cells or solution P3 Primary Cells (V4XP-3024, Lonza) and program DS-120 for KL and LL cells.

tag: cebpa mutational status

value: K313KK LP30


title: KL_siNEG_2


title: KL_siNEG_1


title: KL_siMYB_1


title: S_R56_C5

city: Houston

first: Chad

last: Creighton

state: TX

address: One Baylor Plaza, Mail Stop: BCM305

department: Biostatistics, Ducan Cancer Center

institute: Baylor College of Medicine

description: Single Cell RNA

data_processing:
Sample demultiplexing was performed with custom script to generate sequence file for individual cells
Sequenced reads in FASTQ files were mapped to mm10 whole genome using Tophat (Galaxy tool version 0.9) with parameters -r 100. UCSC known transcripts were supplied in GTF format file.
Fragments Per Kilobase of transcript per Million mapped reads (FPKM) were calculated using Cufflinks (Galaxy tool version 2.2.1.0) with parameters -r 100.  UCSC known transcripts were supplied in GTF format file.
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include UCSC transcript id and their FPKM values along with confidence intervals for each sample.

source_name: mouse prostate cells

growth_protocol: Prostate stromal cells were FACS-isolated from 8-10 week old C57Bl/6 mice based on their surface antigen expression profile (Lin-CD24-CD49f-).

extract_protocol:
Cells were dispensed into printed chip (430-000233, Wafergene) using MultiSample NanoDispenser (MSND).  The chip loaded with cells was imaged using an Olympus BX43 fluorescent microscope to locate wells with single cells using CellSelect software (Takara Bio USA). To break cells for downstream analysis, the chip was frozen at -80°C for more than one hour before thawed.
We employed the iCell8 single cell system from Wafergen BioSystems for single cell capture to achieve an intermediate in-depth expression profiling of single prostate stromal cells.  In-chip RT-PCR amplification and single cell RNA-seq library construction were performed following the iCELL8 single cell polyA transcriptome amplification hands-on workflow checklist and preparation guide.

value: prostate

value: 8-10 weeks


title: S_R56_C57

description: Single Cell RNA


title: S_R57_C25

description: Single Cell RNA


title: S_R56_C66

description: Single Cell RNA


title: S_R56_C50

description: Single Cell RNA


title: S_R56_C70

description: Single Cell RNA


title: S_R56_C61

description: Single Cell RNA


title: S_R56_C8

description: Single Cell RNA


title: S_R57_C12

description: Single Cell RNA


title: S_R56_C71

description: Single Cell RNA


title: Obese mouse 26 ILC1


title: Lean mouse 25 ILC1


title: S_R56_C49

description: Single Cell RNA


title: S_R56_C48

description: Single Cell RNA


title: S_R56_C54

description: Single Cell RNA


title: S_R56_C38

description: Single Cell RNA


title: S_R56_C29

description: Single Cell RNA


title: S_R56_C37

description: Single Cell RNA


title: S_R56_C28

description: Single Cell RNA


title: S_R56_C43

description: Single Cell RNA


title: S_R56_C26

description: Single Cell RNA


title: S_R56_C25

description: Single Cell RNA


title: S_R56_C24

description: Single Cell RNA


title: S_R56_C69

description: Single Cell RNA


title: S_R56_C20

description: Single Cell RNA


title: S_R56_C19

description: Single Cell RNA


title: S_R56_C2

description: Single Cell RNA


title: S_R56_C16

description: Single Cell RNA


title: S_R56_C64

description: Single Cell RNA


title: S_R56_C10

description: Single Cell RNA


title: S_R56_C1

description: Single Cell RNA


title: S_R56_C13

description: Single Cell RNA


title: S_R55_C65

description: Single Cell RNA


title: S_R55_C54

description: Single Cell RNA


title: S_R55_C71

description: Single Cell RNA


title: S_R55_C42

description: Single Cell RNA


title: S_R55_C49

description: Single Cell RNA


title: S_R55_C8

description: Single Cell RNA


title: S_R55_C38

description: Single Cell RNA


title: S_R55_C50

description: Single Cell RNA


title: S_R55_C52

description: Single Cell RNA


title: S_R55_C69

description: Single Cell RNA


title: S_R55_C32

description: Single Cell RNA


title: S_R55_C35

description: Single Cell RNA


title: S_R55_C51

description: Single Cell RNA


title: S_R55_C41

description: Single Cell RNA


title: S_R55_C23

description: Single Cell RNA


title: S_R55_C0

description: Single Cell RNA


title: S_R54_C9

description: Single Cell RNA


title: S_R54_C8

description: Single Cell RNA


title: S_R54_C71

description: Single Cell RNA


title: S_R55_C27

description: Single Cell RNA


title: S_R54_C67

description: Single Cell RNA


title: S_R55_C67

description: Single Cell RNA


title: S_R55_C68

description: Single Cell RNA


title: S_R55_C57

description: Single Cell RNA


title: S_R54_C65

description: Single Cell RNA


title: S_R54_C63

description: Single Cell RNA


title: S_R54_C58

description: Single Cell RNA


title: S_R55_C40

description: Single Cell RNA


title: S_R54_C45

description: Single Cell RNA


title: S_R54_C56

description: Single Cell RNA


title: S_R54_C40

description: Single Cell RNA


title: S_R54_C36

description: Single Cell RNA


title: S_R54_C42

description: Single Cell RNA


title: S_R54_C53

description: Single Cell RNA


title: S_R54_C43

description: Single Cell RNA


title: S_R54_C4

description: Single Cell RNA


title: S_R54_C34

description: Single Cell RNA


title: S_R54_C49

description: Single Cell RNA


title: S_R54_C32

description: Single Cell RNA


title: S_R53_C67

description: Single Cell RNA


title: S_R54_C15

description: Single Cell RNA


title: S_R53_C71

description: Single Cell RNA


title: S_R53_C9

description: Single Cell RNA


title: S_R53_C7

description: Single Cell RNA


title: S_R54_C23

description: Single Cell RNA


title: S_R53_C68

description: Single Cell RNA


title: S_R54_C35

description: Single Cell RNA


title: S_R53_C65

description: Single Cell RNA


title: S_R53_C62

description: Single Cell RNA


title: S_R53_C56

description: Single Cell RNA


title: S_R53_C55

description: Single Cell RNA


title: S_R53_C51

description: Single Cell RNA


title: S_R53_C24

description: Single Cell RNA


title: S_R52_C64

description: Single Cell RNA


title: S_R53_C46

description: Single Cell RNA


title: S_R53_C48

description: Single Cell RNA


title: S_R52_C67

description: Single Cell RNA


title: S_R52_C34

description: Single Cell RNA


title: S_R52_C55

description: Single Cell RNA


title: S_R53_C54

description: Single Cell RNA


title: S_R52_C49

description: Single Cell RNA


title: S_R53_C28

description: Single Cell RNA


title: S_R52_C26

description: Single Cell RNA


title: S_R52_C36

description: Single Cell RNA


title: S_R54_C17

description: Single Cell RNA


title: S_R53_C25

description: Single Cell RNA


title: S_R52_C9

description: Single Cell RNA


title: S_R53_C0

description: Single Cell RNA


title: S_R52_C43

description: Single Cell RNA


title: S_R52_C37

description: Single Cell RNA


title: SR_LA_27

first: Alison

last: Thomas

state: London

address: WHRI, Charterhouse Square

institute: Dr

data_processing:
Sequencing read quality was accessed using FastQC (version 0.11.2). Followed by trimming for adaptor sequences and poor quality reads using Trim Galore (version 0.3.7).
Trimmed sequences were aligned to the human reference genome (GRCh37) using TopHat2 (version 2.0.13) and bowtie2 (version 2.2.3) as backend.
Transcript abundance was then calculated using HTSeq-counts software (version 0.6.0).
Differential expression analysis was investigated using edgeR (version_3.8.6).
Genome_build: GRCh37
Supplementary_files_format_and_content: tab delimited text files of HTSeq-counts showing raw counts per sample

source_name: left atrial appendage

extract_protocol:
RNA was extracted from each sample using QIAGEN Rneasy Mini kit with DnaseI treatment.
Libraries were generated using TruSeq Stranded Total RNA library prep with RiboZero (Illumina, Cat No:RS-122-2203).

treatment_protocol: During surgery a section of atrial appendage was removed and immediately immersed in RNAlater.

value: atrial appendage

tag: Sex

value: Male


title: S_R52_C30

description: Single Cell RNA


title: S_R52_C24

description: Single Cell RNA


title: S_R53_C33

description: Single Cell RNA


title: S_R51_C8

description: Single Cell RNA


title: S_R51_C68

description: Single Cell RNA


title: S_R52_C32

description: Single Cell RNA


title: S_R52_C13

description: Single Cell RNA


title: S_R52_C60

description: Single Cell RNA


title: S_R53_C38

description: Single Cell RNA


title: S_R51_C59

description: Single Cell RNA


title: S_R51_C62

description: Single Cell RNA


title: S_R51_C65

description: Single Cell RNA


title: S_R51_C51

description: Single Cell RNA


title: S_R51_C39

description: Single Cell RNA


title: S_R51_C67

description: Single Cell RNA


title: S_R51_C46

description: Single Cell RNA


title: S_R51_C57

description: Single Cell RNA


title: S_R51_C53

description: Single Cell RNA


title: S_R51_C28

description: Single Cell RNA


title: S_R51_C61

description: Single Cell RNA


title: S_R51_C33

description: Single Cell RNA


title: S_R51_C2

description: Single Cell RNA


title: S_R51_C14

description: Single Cell RNA


title: S_R51_C13

description: Single Cell RNA


title: S_R50_C69

description: Single Cell RNA


title: S_R50_C67

description: Single Cell RNA


title: S_R50_C66

description: Single Cell RNA


title: S_R50_C52

description: Single Cell RNA


title: S_R50_C65

description: Single Cell RNA


title: S_R50_C54

description: Single Cell RNA


title: S_R55_C43

description: Single Cell RNA


title: S_R50_C49

description: Single Cell RNA


title: S_R51_C0

description: Single Cell RNA


title: S_R51_C48

description: Single Cell RNA


title: S_R50_C46

description: Single Cell RNA


title: S_R50_C45

description: Single Cell RNA


title: S_R50_C47

description: Single Cell RNA


title: S_R50_C44

description: Single Cell RNA


title: S_R51_C41

description: Single Cell RNA


title: S_R50_C4

description: Single Cell RNA


title: S_R50_C33

description: Single Cell RNA


title: S_R50_C38

description: Single Cell RNA


title: S_R50_C17

description: Single Cell RNA


title: S_R50_C25

description: Single Cell RNA


title: S_R50_C16

description: Single Cell RNA


title: S_R5_C67

description: Single Cell RNA


title: S_R50_C40

description: Single Cell RNA


title: S_R50_C1

description: Single Cell RNA


title: S_R50_C32

description: Single Cell RNA


title: S_R5_C62

description: Single Cell RNA


title: S_R5_C55

description: Single Cell RNA


title: S_R5_C39

description: Single Cell RNA


title: S_R5_C4

description: Single Cell RNA


title: S_R5_C29

description: Single Cell RNA


title: S_R5_C34

description: Single Cell RNA


title: S_R5_C32

description: Single Cell RNA


title: S_R5_C28

description: Single Cell RNA


title: S_R5_C19

description: Single Cell RNA


title: S_R5_C1

description: Single Cell RNA


title: S_R5_C17

description: Single Cell RNA


title: S_R49_C71

description: Single Cell RNA


title: S_R5_C10

description: Single Cell RNA


title: S_R50_C43

description: Single Cell RNA


title: S_R50_C18

description: Single Cell RNA


title: S_R50_C13

description: Single Cell RNA


title: S_R49_C7

description: Single Cell RNA


title: S_R49_C48

description: Single Cell RNA


title: S_R49_C63

description: Single Cell RNA


title: S_R49_C28

description: Single Cell RNA


title: S_R49_C37

description: Single Cell RNA


title: S_R49_C46

description: Single Cell RNA


title: S_R50_C23

description: Single Cell RNA


title: S_R49_C29

description: Single Cell RNA


title: S_R49_C42

description: Single Cell RNA


title: S_R49_C40

description: Single Cell RNA


title: S_R5_C60

description: Single Cell RNA


title: S_R5_C6

description: Single Cell RNA


title: S_R49_C19

description: Single Cell RNA


title: S_R49_C24

description: Single Cell RNA


title: S_R49_C10

description: Single Cell RNA


title: S_R48_C69

description: Single Cell RNA


title: S_R49_C15

description: Single Cell RNA


title: S_R48_C68

description: Single Cell RNA


title: S_R5_C11

description: Single Cell RNA


title: S_R48_C62

description: Single Cell RNA


title: S_R48_C64

description: Single Cell RNA


title: S_R48_C60

description: Single Cell RNA


title: S_R48_C39

description: Single Cell RNA


title: S_R48_C4

description: Single Cell RNA


title: S_R48_C67

description: Single Cell RNA


title: S_R48_C36

description: Single Cell RNA


title: S_R48_C53

description: Single Cell RNA


title: S_R48_C51

description: Single Cell RNA


title: S_R48_C34

description: Single Cell RNA


title: S_R48_C28

description: Single Cell RNA


title: S_R48_C17

description: Single Cell RNA


title: S_R48_C21

description: Single Cell RNA


title: S_R47_C68

description: Single Cell RNA


title: S_R47_C54

description: Single Cell RNA


title: S_R47_C63

description: Single Cell RNA


title: S_R47_C64

description: Single Cell RNA


title: S_R47_C47

description: Single Cell RNA


title: S_R47_C51

description: Single Cell RNA


title: S_R47_C6

description: Single Cell RNA


title: S_R47_C70

description: Single Cell RNA


title: S_R48_C1

description: Single Cell RNA


title: S_R48_C33

description: Single Cell RNA


title: S_R47_C49

description: Single Cell RNA


title: S_R47_C46

description: Single Cell RNA


title: S_R47_C41

description: Single Cell RNA


title: S_R47_C39

description: Single Cell RNA


title: S_R47_C35

description: Single Cell RNA


title: S_R47_C31

description: Single Cell RNA


title: S_R47_C30

description: Single Cell RNA


title: S_R47_C2

description: Single Cell RNA


title: S_R47_C23

description: Single Cell RNA


title: S_R47_C1

description: Single Cell RNA


title: S_R47_C11

description: Single Cell RNA


title: S_R46_C71

description: Single Cell RNA


title: S_R46_C67

description: Single Cell RNA


title: S_R46_C69

description: Single Cell RNA


title: S_R46_C7

description: Single Cell RNA


title: S_R46_C56

description: Single Cell RNA


title: S_R46_C63

description: Single Cell RNA


title: S_R46_C55

description: Single Cell RNA


title: S_R46_C4

description: Single Cell RNA


title: S_R46_C32

description: Single Cell RNA


title: S_R46_C24

description: Single Cell RNA


title: S_R46_C33

description: Single Cell RNA


title: S_R47_C38

description: Single Cell RNA


title: S_R46_C29

description: Single Cell RNA


title: S_R46_C19

description: Single Cell RNA


title: S_R46_C40

description: Single Cell RNA


title: S_R46_C23

description: Single Cell RNA


title: S_R46_C14

description: Single Cell RNA


title: S_R46_C13

description: Single Cell RNA


title: S_R45_C62

description: Single Cell RNA


title: S_R45_C6

description: Single Cell RNA


title: S_R45_C61

description: Single Cell RNA


title: S_R45_C58

description: Single Cell RNA


title: S_R45_C53

description: Single Cell RNA


title: S_R45_C44

description: Single Cell RNA


title: S_R45_C46

description: Single Cell RNA


title: S_R45_C5

description: Single Cell RNA


title: S_R45_C36

description: Single Cell RNA


title: S_R45_C24

description: Single Cell RNA


title: S_R45_C38

description: Single Cell RNA


title: S_R45_C29

description: Single Cell RNA


title: S_R45_C25

description: Single Cell RNA


title: S_R45_C13

description: Single Cell RNA


title: S_R45_C19

description: Single Cell RNA


title: S_R46_C50

description: Single Cell RNA


title: S_R46_C20

description: Single Cell RNA


title: S_R45_C15

description: Single Cell RNA


title: S_R44_C45

description: Single Cell RNA


title: S_R44_C41

description: Single Cell RNA


title: S_R44_C71

description: Single Cell RNA


title: S_R45_C68

description: Single Cell RNA


title: S_R45_C11

description: Single Cell RNA


title: S_R45_C12

description: Single Cell RNA


title: S_R44_C57

description: Single Cell RNA


title: S_R44_C53

description: Single Cell RNA


title: S_R44_C37

description: Single Cell RNA


title: S_R44_C33

description: Single Cell RNA


title: S_R44_C18

description: Single Cell RNA


title: S_R44_C14

description: Single Cell RNA


title: S_R44_C11

description: Single Cell RNA


title: S_R44_C31

description: Single Cell RNA


title: S_R44_C40

description: Single Cell RNA


title: S_R43_C71

description: Single Cell RNA


title: S_R43_C70

description: Single Cell RNA


title: S_R43_C46

description: Single Cell RNA


title: S_R43_C40

description: Single Cell RNA


title: S_R43_C35

description: Single Cell RNA


title: S_R43_C43

description: Single Cell RNA


title: S_R44_C64

description: Single Cell RNA


title: S_R43_C37

description: Single Cell RNA


title: S_R43_C36

description: Single Cell RNA


title: S_R43_C2

description: Single Cell RNA


title: S_R42_C58

description: Single Cell RNA


title: S_R43_C14

description: Single Cell RNA


title: S_R42_C59

description: Single Cell RNA


title: S_R43_C15

description: Single Cell RNA


title: S_R43_C19

description: Single Cell RNA


title: S_R42_C63

description: Single Cell RNA


title: S_R43_C16

description: Single Cell RNA


title: S_R42_C61

description: Single Cell RNA


title: S_R43_C17

description: Single Cell RNA


title: S_R42_C51

description: Single Cell RNA


title: S_R42_C53

description: Single Cell RNA


title: S_R42_C50

description: Single Cell RNA


title: S_R42_C48

description: Single Cell RNA


title: S_R42_C5

description: Single Cell RNA


title: S_R43_C62

description: Single Cell RNA


title: S_R42_C52

description: Single Cell RNA


title: S_R42_C54

description: Single Cell RNA


title: S_R42_C43

description: Single Cell RNA


title: S_R42_C34

description: Single Cell RNA


title: S_R42_C36

description: Single Cell RNA


title: S_R43_C28

description: Single Cell RNA


title: S_R42_C30

description: Single Cell RNA


title: S_R43_C24

description: Single Cell RNA


title: S_R42_C17

description: Single Cell RNA


title: S_R42_C22

description: Single Cell RNA


title: S_R42_C16

description: Single Cell RNA


title: S_R42_C13

description: Single Cell RNA


title: S_R41_C46

description: Single Cell RNA


title: S_R41_C9

description: Single Cell RNA


title: S_R41_C45

description: Single Cell RNA


title: S_R42_C46

description: Single Cell RNA


title: S_R41_C44

description: Single Cell RNA


title: S_R41_C53

description: Single Cell RNA


title: S_R41_C61

description: Single Cell RNA


title: S_R42_C0

description: Single Cell RNA


title: S_R41_C34

description: Single Cell RNA


title: S_R41_C36

description: Single Cell RNA


title: S_R41_C27

description: Single Cell RNA


title: S_R41_C0

description: Single Cell RNA


title: S_R41_C2

description: Single Cell RNA


title: S_R40_C71

description: Single Cell RNA


title: S_R40_C70

description: Single Cell RNA


title: S_R40_C64

description: Single Cell RNA


title: S_R40_C59

description: Single Cell RNA


title: S_R40_C58

description: Single Cell RNA


title: S_R40_C55

description: Single Cell RNA


title: S_R40_C52

description: Single Cell RNA


title: S_R40_C6

description: Single Cell RNA


title: S_R42_C42

description: Single Cell RNA


title: S_R40_C51

description: Single Cell RNA


title: S_R40_C44

description: Single Cell RNA


title: S_R41_C29

description: Single Cell RNA


title: S_R41_C41

description: Single Cell RNA


title: S_R40_C49

description: Single Cell RNA


title: S_R40_C43

description: Single Cell RNA


title: S_R41_C12

description: Single Cell RNA


title: S_R40_C1

description: Single Cell RNA


title: S_R40_C30

description: Single Cell RNA


title: S_R40_C16

description: Single Cell RNA


title: S_R40_C0

description: Single Cell RNA


title: S_R40_C31

description: Single Cell RNA


title: S_R40_C27

description: Single Cell RNA


title: S_R40_C67

description: Single Cell RNA


title: S_R40_C13

description: Single Cell RNA


title: S_R40_C41

description: Single Cell RNA


title: S_R40_C24

description: Single Cell RNA


title: S_R4_C46

description: Single Cell RNA


title: S_R4_C58

description: Single Cell RNA


title: S_R4_C61

description: Single Cell RNA


title: S_R4_C43

description: Single Cell RNA


title: S_R4_C44

description: Single Cell RNA


title: S_R4_C41

description: Single Cell RNA


title: S_R4_C31

description: Single Cell RNA


title: S_R4_C29

description: Single Cell RNA


title: S_R40_C66

description: Single Cell RNA


title: S_R4_C22

description: Single Cell RNA


title: S_R4_C35

description: Single Cell RNA


title: S_R4_C3

description: Single Cell RNA


title: S_R4_C19

description: Single Cell RNA


title: S_R39_C48

description: Single Cell RNA


title: S_R39_C63

description: Single Cell RNA


title: S_R39_C61

description: Single Cell RNA


title: S_R4_C47

description: Single Cell RNA


title: S_R39_C41

description: Single Cell RNA


title: S_R39_C21

description: Single Cell RNA


title: S_R39_C36

description: Single Cell RNA


title: S_R39_C47

description: Single Cell RNA


title: S_R39_C27

description: Single Cell RNA


title: S_R39_C20

description: Single Cell RNA


title: S_R39_C51

description: Single Cell RNA


title: S_R39_C2

description: Single Cell RNA


title: S_R38_C68

description: Single Cell RNA


title: S_R38_C7

description: Single Cell RNA


title: S_R39_C33

description: Single Cell RNA


title: S_R38_C61

description: Single Cell RNA


title: S_R38_C66

description: Single Cell RNA


title: S_R38_C65

description: Single Cell RNA


title: S_R38_C49

description: Single Cell RNA


title: S_R38_C51

description: Single Cell RNA


title: S_R38_C47

description: Single Cell RNA


title: S_R39_C66

description: Single Cell RNA


title: S_R38_C45

description: Single Cell RNA


title: S_R38_C54

description: Single Cell RNA


title: S_R38_C42

description: Single Cell RNA


title: S_R4_C13

description: Single Cell RNA


title: S_R38_C46

description: Single Cell RNA


title: S_R38_C36

description: Single Cell RNA


title: S_R38_C40

description: Single Cell RNA


title: S_R39_C14

description: Single Cell RNA


title: S_R38_C17

description: Single Cell RNA


title: S_R38_C24

description: Single Cell RNA


title: S_R38_C35

description: Single Cell RNA


title: S_R38_C22

description: Single Cell RNA


title: S_R38_C26

description: Single Cell RNA


title: S_R38_C12

description: Single Cell RNA


title: S_R38_C28

description: Single Cell RNA


title: S_R37_C7

description: Single Cell RNA


title: S_R37_C70

description: Single Cell RNA


title: S_R38_C15

description: Single Cell RNA


title: S_R37_C64

description: Single Cell RNA


title: S_R37_C65

description: Single Cell RNA


title: S_R37_C68

description: Single Cell RNA


title: S_R38_C19

description: Single Cell RNA


title: S_R37_C60

description: Single Cell RNA


title: S_R38_C34

description: Single Cell RNA


title: S_R37_C59

description: Single Cell RNA


title: S_R37_C56

description: Single Cell RNA


title: S_R37_C52

description: Single Cell RNA


title: S_R37_C51

description: Single Cell RNA


title: S_R37_C47

description: Single Cell RNA


title: S_R37_C40

description: Single Cell RNA


title: S_R37_C26

description: Single Cell RNA


title: S_R37_C34

description: Single Cell RNA


title: S_R38_C16

description: Single Cell RNA


title: S_R37_C25

description: Single Cell RNA


title: S_R37_C17

description: Single Cell RNA


title: S_R37_C19

description: Single Cell RNA


title: S_R36_C68

description: Single Cell RNA


title: S_R36_C63

description: Single Cell RNA


title: S_R36_C61

description: Single Cell RNA


title: S_R36_C65

description: Single Cell RNA


title: S_R36_C57

description: Single Cell RNA


title: S_R36_C54

description: Single Cell RNA


title: S_R38_C9

description: Single Cell RNA


title: S_R36_C34

description: Single Cell RNA


title: S_R36_C5

description: Single Cell RNA


title: S_R36_C33

description: Single Cell RNA


title: S_R36_C45

description: Single Cell RNA


title: S_R37_C46

description: Single Cell RNA


title: S_R37_C35

description: Single Cell RNA


title: S_R36_C18

description: Single Cell RNA


title: S_R39_C43

description: Single Cell RNA


title: S_R36_C46

description: Single Cell RNA


title: S_R36_C30

description: Single Cell RNA


title: S_R36_C11

description: Single Cell RNA


title: S_R36_C13

description: Single Cell RNA


title: S_R37_C14

description: Single Cell RNA


title: S_R36_C25

description: Single Cell RNA


title: S_R36_C26

description: Single Cell RNA


title: S_R36_C1

description: Single Cell RNA


title: S_R37_C16

description: Single Cell RNA


title: S_R35_C66

description: Single Cell RNA


title: S_R35_C70

description: Single Cell RNA


title: S_R35_C8

description: Single Cell RNA


title: S_R35_C64

description: Single Cell RNA


title: S_R35_C65

description: Single Cell RNA


title: S_R35_C53

description: Single Cell RNA


title: S_R35_C50

description: Single Cell RNA


title: S_R35_C56

description: Single Cell RNA


title: S_R35_C5

description: Single Cell RNA


title: S_R35_C40

description: Single Cell RNA


title: S_R35_C46

description: Single Cell RNA


title: S_R36_C28

description: Single Cell RNA


title: S_R35_C38

description: Single Cell RNA


title: S_R35_C31

description: Single Cell RNA


title: S_R35_C35

description: Single Cell RNA


title: S_R35_C29

description: Single Cell RNA


title: S_R35_C3

description: Single Cell RNA


title: S_R35_C17

description: Single Cell RNA


title: S_R35_C25

description: Single Cell RNA


title: S_R35_C32

description: Single Cell RNA


title: S_R35_C18

description: Single Cell RNA


title: S_R35_C30

description: Single Cell RNA


title: S_R34_C70

description: Single Cell RNA


title: S_R35_C12

description: Single Cell RNA


title: S_R35_C39

description: Single Cell RNA


title: S_R35_C16

description: Single Cell RNA


title: S_R34_C63

description: Single Cell RNA


title: S_R34_C59

description: Single Cell RNA


title: S_R34_C57

description: Single Cell RNA


title: S_R34_C53

description: Single Cell RNA


title: S_R34_C5

description: Single Cell RNA


title: S_R34_C45

description: Single Cell RNA


title: S_R34_C31

description: Single Cell RNA


title: S_R34_C24

description: Single Cell RNA


title: S_R34_C10

description: Single Cell RNA


title: S_R33_C64

description: Single Cell RNA


title: S_R33_C59

description: Single Cell RNA


title: S_R33_C52

description: Single Cell RNA


title: S_R35_C2

description: Single Cell RNA


title: S_R34_C7

description: Single Cell RNA


title: S_R33_C48

description: Single Cell RNA


title: S_R33_C49

description: Single Cell RNA


title: S_R33_C34

description: Single Cell RNA


title: S_R33_C25

description: Single Cell RNA


title: S_R33_C44

description: Single Cell RNA


title: S_R33_C37

description: Single Cell RNA


title: S_R33_C23

description: Single Cell RNA


title: S_R32_C70

description: Single Cell RNA


title: S_R33_C35

description: Single Cell RNA


title: S_R34_C41

description: Single Cell RNA


title: S_R32_C69

description: Single Cell RNA


title: S_R32_C60

description: Single Cell RNA


title: S_R32_C56

description: Single Cell RNA


title: S_R32_C66

description: Single Cell RNA


title: S_R32_C57

description: Single Cell RNA


title: S_R34_C39

description: Single Cell RNA


title: S_R32_C62

description: Single Cell RNA


title: S_R34_C21

description: Single Cell RNA


title: S_R32_C47

description: Single Cell RNA


title: S_R32_C36

description: Single Cell RNA


title: S_R33_C7

description: Single Cell RNA


title: S_R33_C5

description: Single Cell RNA


title: S_R32_C24

description: Single Cell RNA


title: S_R32_C25

description: Single Cell RNA


title: S_R32_C29

description: Single Cell RNA


title: S_R32_C14

description: Single Cell RNA


title: S_R32_C18

description: Single Cell RNA


title: S_R33_C18

description: Single Cell RNA


title: S_R32_C10

description: Single Cell RNA


title: S_R32_C13

description: Single Cell RNA


title: S_R31_C70

description: Single Cell RNA


title: S_R31_C68

description: Single Cell RNA


title: S_R31_C54

description: Single Cell RNA


title: S_R31_C60

description: Single Cell RNA


title: S_R31_C37

description: Single Cell RNA


title: S_R31_C41

description: Single Cell RNA


title: S_R31_C55

description: Single Cell RNA


title: S_R31_C63

description: Single Cell RNA


title: S_R31_C6

description: Single Cell RNA


title: S_R31_C34

description: Single Cell RNA


title: S_R31_C3

description: Single Cell RNA


title: S_R31_C15

description: Single Cell RNA


title: S_R31_C22

description: Single Cell RNA


title: S_R31_C29

description: Single Cell RNA


title: S_R31_C24

description: Single Cell RNA


title: S_R31_C27

description: Single Cell RNA


title: S_R31_C16

description: Single Cell RNA


title: S_R31_C25

description: Single Cell RNA


title: S_R31_C13

description: Single Cell RNA


title: S_R31_C11

description: Single Cell RNA


title: S_R30_C66

description: Single Cell RNA


title: S_R30_C9

description: Single Cell RNA


title: S_R30_C65

description: Single Cell RNA


title: S_R30_C61

description: Single Cell RNA


title: S_R30_C56

description: Single Cell RNA


title: S_R30_C54

description: Single Cell RNA


title: S_R30_C52

description: Single Cell RNA


title: S_R30_C5

description: Single Cell RNA


title: S_R30_C48

description: Single Cell RNA


title: S_R30_C44

description: Single Cell RNA


title: S_R30_C43

description: Single Cell RNA


title: S_R30_C29

description: Single Cell RNA


title: S_R30_C4

description: Single Cell RNA


title: S_R30_C36

description: Single Cell RNA


title: S_R30_C30

description: Single Cell RNA


title: S_R30_C28

description: Single Cell RNA


title: S_R30_C3

description: Single Cell RNA


title: S_R30_C40

description: Single Cell RNA


title: S_R30_C27

description: Single Cell RNA


title: S_R30_C22

description: Single Cell RNA


title: S_R30_C20

description: Single Cell RNA


title: S_R30_C13

description: Single Cell RNA


title: S_R3_C67

description: Single Cell RNA


title: S_R30_C0

description: Single Cell RNA


title: S_R30_C17

description: Single Cell RNA


title: S_R30_C62

description: Single Cell RNA


title: S_R30_C14

description: Single Cell RNA


title: S_R3_C38

description: Single Cell RNA


title: S_R3_C30

description: Single Cell RNA


title: S_R3_C15

description: Single Cell RNA


title: S_R3_C27

description: Single Cell RNA


title: S_R3_C13

description: Single Cell RNA


title: S_R3_C0

description: Single Cell RNA


title: S_R30_C1

description: Single Cell RNA


title: S_R3_C1

description: Single Cell RNA


title: S_R29_C71

description: Single Cell RNA


title: S_R29_C65

description: Single Cell RNA


title: S_R29_C56

description: Single Cell RNA


title: S_R29_C46

description: Single Cell RNA


title: S_R29_C54

description: Single Cell RNA


title: S_R29_C44

description: Single Cell RNA


title: S_R29_C55

description: Single Cell RNA


title: S_R29_C43

description: Single Cell RNA


title: S_R29_C4

description: Single Cell RNA


title: S_R29_C33

description: Single Cell RNA


title: S_R3_C54

description: Single Cell RNA


title: S_R29_C28

description: Single Cell RNA


title: S_R29_C25

description: Single Cell RNA


title: S_R29_C22

description: Single Cell RNA


title: S_R29_C21

description: Single Cell RNA


title: S_R3_C42

description: Single Cell RNA


title: S_R3_C12

description: Single Cell RNA


title: S_R29_C16

description: Single Cell RNA


title: S_R29_C62

description: Single Cell RNA


title: S_R29_C14

description: Single Cell RNA


title: S_R28_C8

description: Single Cell RNA


title: S_R29_C11

description: Single Cell RNA


title: S_R29_C13

description: Single Cell RNA


title: S_R28_C45

description: Single Cell RNA


title: S_R28_C48

description: Single Cell RNA


title: S_R28_C49

description: Single Cell RNA


title: S_R28_C44

description: Single Cell RNA


title: S_R28_C39

description: Single Cell RNA


title: S_R28_C63

description: Single Cell RNA


title: S_R28_C36

description: Single Cell RNA


title: S_R28_C31

description: Single Cell RNA


title: S_R28_C30

description: Single Cell RNA


title: S_R28_C43

description: Single Cell RNA


title: S_R28_C23

description: Single Cell RNA


title: S_R28_C12

description: Single Cell RNA


title: S_R28_C17

description: Single Cell RNA


title: S_R28_C34

description: Single Cell RNA


title: S_R27_C67

description: Single Cell RNA


title: S_R27_C65

description: Single Cell RNA


title: S_R27_C8

description: Single Cell RNA


title: S_R27_C53

description: Single Cell RNA


title: S_R27_C50

description: Single Cell RNA


title: S_R27_C45

description: Single Cell RNA


title: S_R27_C46

description: Single Cell RNA


title: S_R27_C35

description: Single Cell RNA


title: S_R28_C47

description: Single Cell RNA


title: S_R27_C19

description: Single Cell RNA


title: S_R27_C26

description: Single Cell RNA


title: S_R27_C34

description: Single Cell RNA


title: S_R27_C17

description: Single Cell RNA


title: S_R26_C61

description: Single Cell RNA


title: S_R27_C14

description: Single Cell RNA


title: S_R26_C69

description: Single Cell RNA


title: S_R27_C13

description: Single Cell RNA


title: S_R26_C68

description: Single Cell RNA


title: S_R26_C47

description: Single Cell RNA


title: S_R26_C59

description: Single Cell RNA


title: S_R26_C46

description: Single Cell RNA


title: S_R26_C51

description: Single Cell RNA


title: S_R26_C53

description: Single Cell RNA


title: S_R27_C58

description: Single Cell RNA


title: S_R26_C34

description: Single Cell RNA


title: S_R26_C33

description: Single Cell RNA


title: S_R26_C4

description: Single Cell RNA


title: S_R26_C32

description: Single Cell RNA


title: S_R26_C23

description: Single Cell RNA


title: S_R26_C44

description: Single Cell RNA


title: S_R26_C13

description: Single Cell RNA


title: S_R26_C25

description: Single Cell RNA


title: S_R26_C1

description: Single Cell RNA


title: S_R25_C65

description: Single Cell RNA


title: S_R26_C42

description: Single Cell RNA


title: S_R25_C71

description: Single Cell RNA


title: S_R25_C61

description: Single Cell RNA


title: S_R26_C18

description: Single Cell RNA


title: S_R25_C58

description: Single Cell RNA


title: S_R25_C53

description: Single Cell RNA


title: S_R25_C48

description: Single Cell RNA


title: S_R25_C4

description: Single Cell RNA


title: S_R25_C55

description: Single Cell RNA


title: S_R25_C45

description: Single Cell RNA


title: S_R25_C36

description: Single Cell RNA


title: S_R25_C35

description: Single Cell RNA


title: S_R25_C11

description: Single Cell RNA


title: S_R25_C33

description: Single Cell RNA


title: S_R25_C13

description: Single Cell RNA


title: S_R25_C10

description: Single Cell RNA


title: S_R25_C0

description: Single Cell RNA


title: S_R25_C1

description: Single Cell RNA


title: S_R25_C68

description: Single Cell RNA


title: S_R24_C64

description: Single Cell RNA


title: S_R24_C58

description: Single Cell RNA


title: S_R24_C9

description: Single Cell RNA


title: S_R24_C53

description: Single Cell RNA


title: S_R24_C52

description: Single Cell RNA


title: S_R24_C43

description: Single Cell RNA


title: S_R24_C38

description: Single Cell RNA


title: S_R24_C3

description: Single Cell RNA


title: S_R24_C36

description: Single Cell RNA


title: S_R24_C27

description: Single Cell RNA


title: S_R24_C24

description: Single Cell RNA


title: S_R24_C22

description: Single Cell RNA


title: S_R24_C17

description: Single Cell RNA


title: S_R25_C46

description: Single Cell RNA


title: S_R24_C16

description: Single Cell RNA


title: S_R24_C13

description: Single Cell RNA


title: S_R24_C11

description: Single Cell RNA


title: S_R25_C26

description: Single Cell RNA


title: S_R25_C17

description: Single Cell RNA


title: S_R23_C8

description: Single Cell RNA


title: S_R24_C0

description: Single Cell RNA


title: S_R23_C63

description: Single Cell RNA


title: S_R23_C68

description: Single Cell RNA


title: S_R23_C6

description: Single Cell RNA


title: S_R23_C59

description: Single Cell RNA


title: S_R23_C5

description: Single Cell RNA


title: S_R23_C46

description: Single Cell RNA


title: S_R23_C58

description: Single Cell RNA


title: S_R23_C55

description: Single Cell RNA


title: S_R23_C37

description: Single Cell RNA


title: S_R23_C31

description: Single Cell RNA


title: S_R23_C34

description: Single Cell RNA


title: S_R24_C57

description: Single Cell RNA


title: S_R23_C24

description: Single Cell RNA


title: S_R23_C25

description: Single Cell RNA


title: S_R23_C27

description: Single Cell RNA


title: S_R23_C21

description: Single Cell RNA


title: S_R23_C14

description: Single Cell RNA


title: S_R22_C70

description: Single Cell RNA


title: S_R22_C60

description: Single Cell RNA


title: S_R22_C54

description: Single Cell RNA


title: S_R23_C11

description: Single Cell RNA


title: S_R22_C52

description: Single Cell RNA


title: S_R22_C8

description: Single Cell RNA


title: S_R22_C67

description: Single Cell RNA


title: S_R22_C5

description: Single Cell RNA


title: S_R23_C53

description: Single Cell RNA


title: S_R22_C43

description: Single Cell RNA


title: S_R22_C42

description: Single Cell RNA


title: S_R22_C46

description: Single Cell RNA


title: S_R23_C47

description: Single Cell RNA


title: S_R23_C40

description: Single Cell RNA


title: S_R22_C39

description: Single Cell RNA


title: S_R22_C4

description: Single Cell RNA


title: S_R22_C34

description: Single Cell RNA


title: S_R22_C24

description: Single Cell RNA


title: S_R22_C32

description: Single Cell RNA


title: S_R22_C20

description: Single Cell RNA


title: S_R22_C28

description: Single Cell RNA


title: S_R22_C19

description: Single Cell RNA


title: S_R22_C16

description: Single Cell RNA


title: S_R22_C18

description: Single Cell RNA


title: S_R22_C12

description: Single Cell RNA


title: S_R22_C47

description: Single Cell RNA


title: S_R23_C20

description: Single Cell RNA


title: S_R22_C49

description: Single Cell RNA


title: S_R21_C8

description: Single Cell RNA


title: S_R22_C11

description: Single Cell RNA


title: S_R21_C9

description: Single Cell RNA


title: S_R21_C65

description: Single Cell RNA


title: S_R21_C70

description: Single Cell RNA


title: S_R21_C53

description: Single Cell RNA


title: S_R21_C6

description: Single Cell RNA


title: S_R21_C55

description: Single Cell RNA


title: S_R21_C48

description: Single Cell RNA


title: S_R21_C45

description: Single Cell RNA


title: S_R21_C38

description: Single Cell RNA


title: S_R21_C40

description: Single Cell RNA


title: S_R21_C39

description: Single Cell RNA


title: S_R21_C31

description: Single Cell RNA


title: S_R21_C36

description: Single Cell RNA


title: S_R22_C25

description: Single Cell RNA


title: S_R21_C3

description: Single Cell RNA


title: S_R21_C28

description: Single Cell RNA


title: S_R21_C19

description: Single Cell RNA


title: S_R21_C14

description: Single Cell RNA


title: S_R21_C0

description: Single Cell RNA


title: S_R21_C27

description: Single Cell RNA


title: S_R21_C11

description: Single Cell RNA


title: S_R20_C68

description: Single Cell RNA


title: S_R21_C18

description: Single Cell RNA


title: S_R20_C66

description: Single Cell RNA


title: S_R20_C63

description: Single Cell RNA


title: S_R20_C55

description: Single Cell RNA


title: S_R20_C61

description: Single Cell RNA


title: S_R20_C53

description: Single Cell RNA


title: S_R20_C56

description: Single Cell RNA


title: S_R20_C47

description: Single Cell RNA


title: S_R20_C45

description: Single Cell RNA


title: S_R20_C43

description: Single Cell RNA


title: S_R20_C37

description: Single Cell RNA


title: S_R20_C41

description: Single Cell RNA


title: S_R20_C27

description: Single Cell RNA


title: S_R20_C30

description: Single Cell RNA


title: S_R21_C23

description: Single Cell RNA


title: S_R20_C4

description: Single Cell RNA


title: S_R20_C36

description: Single Cell RNA


title: S_R20_C14

description: Single Cell RNA


title: S_R20_C12

description: Single Cell RNA


title: S_R20_C24

description: Single Cell RNA


title: S_R20_C20

description: Single Cell RNA


title: S_R20_C26

description: Single Cell RNA


title: S_R20_C10

description: Single Cell RNA


title: S_R2_C53

description: Single Cell RNA


title: S_R2_C55

description: Single Cell RNA


title: S_R2_C47

description: Single Cell RNA


title: S_R2_C35

description: Single Cell RNA


title: S_R2_C29

description: Single Cell RNA


title: S_R2_C27

description: Single Cell RNA


title: S_R2_C57

description: Single Cell RNA


title: S_R2_C23

description: Single Cell RNA


title: S_R2_C14

description: Single Cell RNA


title: S_R2_C18

description: Single Cell RNA


title: S_R19_C8

description: Single Cell RNA


title: S_R19_C61

description: Single Cell RNA


title: S_R20_C5

description: Single Cell RNA


title: S_R2_C1

description: Single Cell RNA


title: S_R20_C3

description: Single Cell RNA


title: S_R19_C5

description: Single Cell RNA


title: S_R19_C4

description: Single Cell RNA


title: S_R19_C29

description: Single Cell RNA


title: S_R19_C51

description: Single Cell RNA


title: S_R19_C26

description: Single Cell RNA


title: S_R19_C43

description: Single Cell RNA


title: S_R19_C25

description: Single Cell RNA


title: S_R19_C16

description: Single Cell RNA


title: S_R19_C14

description: Single Cell RNA


title: S_R19_C37

description: Single Cell RNA


title: S_R19_C22

description: Single Cell RNA


title: S_R19_C12

description: Single Cell RNA


title: S_R18_C70

description: Single Cell RNA


title: S_R19_C10

description: Single Cell RNA


title: S_R19_C70

description: Single Cell RNA


title: S_R18_C16

description: Single Cell RNA


title: S_R18_C22

description: Single Cell RNA


title: S_R18_C10

description: Single Cell RNA


title: S_R17_C62

description: Single Cell RNA


title: S_R18_C0

description: Single Cell RNA


title: S_R17_C9

description: Single Cell RNA


title: S_R17_C6

description: Single Cell RNA


title: S_R17_C49

description: Single Cell RNA


title: S_R17_C36

description: Single Cell RNA


title: S_R17_C37

description: Single Cell RNA


title: S_R17_C43

description: Single Cell RNA


title: S_R17_C35

description: Single Cell RNA


title: S_R17_C30

description: Single Cell RNA


title: S_R17_C31

description: Single Cell RNA


title: S_R18_C33

description: Single Cell RNA


title: S_R21_C43

description: Single Cell RNA


title: S_R17_C61

description: Single Cell RNA


title: S_R18_C15

description: Single Cell RNA


title: S_R17_C27

description: Single Cell RNA


title: S_R17_C44

description: Single Cell RNA


title: S_R17_C13

description: Single Cell RNA


title: S_R16_C68

description: Single Cell RNA


title: S_R16_C53

description: Single Cell RNA


title: S_R18_C47

description: Single Cell RNA


title: S_R17_C10

description: Single Cell RNA


title: S_R16_C4

description: Single Cell RNA


title: S_R16_C36

description: Single Cell RNA


title: S_R18_C36

description: Single Cell RNA


title: S_R18_C26

description: Single Cell RNA


title: S_R17_C48

description: Single Cell RNA


title: S_R16_C62

description: Single Cell RNA


title: S_R16_C52

description: Single Cell RNA


title: S_R16_C50

description: Single Cell RNA


title: S_R18_C11

description: Single Cell RNA


title: S_R16_C46

description: Single Cell RNA


title: S_R16_C34

description: Single Cell RNA


title: S_R16_C32

description: Single Cell RNA


title: S_R16_C31

description: Single Cell RNA


title: S_R16_C33

description: Single Cell RNA


title: S_R16_C24

description: Single Cell RNA


title: S_R16_C3

description: Single Cell RNA


title: S_R16_C15

description: Single Cell RNA


title: S_R16_C19

description: Single Cell RNA


title: S_R16_C27

description: Single Cell RNA


title: S_R15_C58

description: Single Cell RNA


title: S_R16_C65

description: Single Cell RNA


title: S_R15_C56

description: Single Cell RNA


title: S_R16_C0

description: Single Cell RNA


title: S_R15_C54

description: Single Cell RNA


title: S_R16_C22

description: Single Cell RNA


title: S_R15_C9

description: Single Cell RNA


title: S_R15_C42

description: Single Cell RNA


title: S_R15_C31

description: Single Cell RNA


title: S_R15_C41

description: Single Cell RNA


title: S_R15_C51

description: Single Cell RNA


title: S_R15_C43

description: Single Cell RNA


title: S_R15_C60

description: Single Cell RNA


title: S_R15_C46

description: Single Cell RNA


title: S_R15_C23

description: Single Cell RNA


title: S_R15_C36

description: Single Cell RNA


title: S_R15_C18

description: Single Cell RNA


title: S_R15_C20

description: Single Cell RNA


title: S_R15_C19

description: Single Cell RNA


title: S_R15_C40

description: Single Cell RNA


title: S_R15_C4

description: Single Cell RNA


title: S_R15_C14

description: Single Cell RNA


title: S_R15_C1

description: Single Cell RNA


title: S_R14_C9

description: Single Cell RNA


title: S_R14_C60

description: Single Cell RNA


title: S_R14_C57

description: Single Cell RNA


title: S_R14_C56

description: Single Cell RNA


title: S_R14_C54

description: Single Cell RNA


title: S_R14_C42

description: Single Cell RNA


title: S_R14_C44

description: Single Cell RNA


title: S_R14_C66

description: Single Cell RNA


title: S_R14_C31

description: Single Cell RNA


title: S_R14_C40

description: Single Cell RNA


title: S_R14_C2

description: Single Cell RNA


title: S_R14_C19

description: Single Cell RNA


title: S_R14_C10

description: Single Cell RNA


title: S_R13_C7

description: Single Cell RNA


title: S_R14_C0

description: Single Cell RNA


title: S_R13_C66

description: Single Cell RNA


title: S_R13_C63

description: Single Cell RNA


title: S_R15_C13

description: Single Cell RNA


title: S_R13_C51

description: Single Cell RNA


title: S_R13_C43

description: Single Cell RNA


title: S_R13_C41

description: Single Cell RNA


title: S_R13_C44

description: Single Cell RNA


title: S_R13_C4

description: Single Cell RNA


title: S_R13_C40

description: Single Cell RNA


title: S_R13_C59

description: Single Cell RNA


title: S_R13_C48

description: Single Cell RNA


title: S_R13_C36

description: Single Cell RNA


title: S_R13_C31

description: Single Cell RNA


title: S_R14_C50

description: Single Cell RNA


title: S_R13_C32

description: Single Cell RNA


title: S_R13_C24

description: Single Cell RNA


title: S_R14_C37

description: Single Cell RNA


title: S_R13_C11

description: Single Cell RNA


title: S_R13_C19

description: Single Cell RNA


title: S_R13_C0

description: Single Cell RNA


title: S_R12_C61

description: Single Cell RNA


title: S_R13_C30

description: Single Cell RNA


title: S_R13_C70

description: Single Cell RNA


title: S_R12_C51

description: Single Cell RNA


title: S_R12_C4

description: Single Cell RNA


title: S_R12_C39

description: Single Cell RNA


title: S_R12_C47

description: Single Cell RNA


title: S_R12_C59

description: Single Cell RNA


title: S_R12_C5

description: Single Cell RNA


title: S_R12_C32

description: Single Cell RNA


title: S_R12_C19

description: Single Cell RNA


title: S_R12_C10

description: Single Cell RNA


title: S_R11_C64

description: Single Cell RNA


title: S_R11_C68

description: Single Cell RNA


title: S_R12_C13

description: Single Cell RNA


title: S_R11_C67

description: Single Cell RNA


title: S_R12_C21

description: Single Cell RNA


title: S_R11_C52

description: Single Cell RNA


title: S_R11_C62

description: Single Cell RNA


title: S_R11_C55

description: Single Cell RNA


title: S_R11_C5

description: Single Cell RNA


title: S_R11_C35

description: Single Cell RNA


title: S_R11_C44

description: Single Cell RNA


title: S_R11_C4

description: Single Cell RNA


title: S_R11_C33

description: Single Cell RNA


title: S_R11_C26

description: Single Cell RNA


title: S_R12_C55

description: Single Cell RNA


title: S_R11_C24

description: Single Cell RNA


title: S_R11_C19

description: Single Cell RNA


title: S_R11_C17

description: Single Cell RNA


title: S_R11_C16

description: Single Cell RNA


title: S_R11_C13

description: Single Cell RNA


title: S_R10_C9

description: Single Cell RNA


title: S_R11_C10

description: Single Cell RNA


title: S_R11_C0

description: Single Cell RNA


title: S_R10_C68

description: Single Cell RNA


title: S_R10_C63

description: Single Cell RNA


title: S_R10_C61

description: Single Cell RNA


title: S_R10_C49

description: Single Cell RNA


title: S_R10_C56

description: Single Cell RNA


title: S_R10_C23

description: Single Cell RNA


title: S_R10_C28

description: Single Cell RNA


title: S_R10_C2

description: Single Cell RNA


title: S_R10_C22

description: Single Cell RNA


title: S_R10_C40

description: Single Cell RNA


title: S_R10_C16

description: Single Cell RNA


title: S_R10_C10

description: Single Cell RNA


title: S_R10_C17

description: Single Cell RNA


title: S_R10_C15

description: Single Cell RNA


title: S_R10_C13

description: Single Cell RNA


title: S_R11_C23

description: Single Cell RNA


title: S_R10_C1

description: Single Cell RNA


title: S_R10_C0

description: Single Cell RNA


title: S_R1_C6

description: Single Cell RNA


title: S_R1_C7

description: Single Cell RNA


title: S_R1_C47

description: Single Cell RNA


title: S_R1_C49

description: Single Cell RNA


title: S_R1_C36

description: Single Cell RNA


title: S_R1_C43

description: Single Cell RNA


title: S_R1_C10

description: Single Cell RNA


title: S_R11_C1

description: Single Cell RNA


title: S_R0_C57

description: Single Cell RNA


title: S_R0_C6

description: Single Cell RNA


title: S_R0_C54

description: Single Cell RNA


title: S_R0_C55

description: Single Cell RNA


title: S_R0_C51

description: Single Cell RNA


title: S_R10_C44

description: Single Cell RNA


title: S_R0_C46

description: Single Cell RNA


title: S_R0_C41

description: Single Cell RNA


title: S_R0_C38

description: Single Cell RNA


title: S_R0_C34

description: Single Cell RNA


title: S_R0_C44

description: Single Cell RNA


title: S_R0_C19

description: Single Cell RNA


title: S_R0_C20

description: Single Cell RNA


title: Pos Ctrl_R67_C69

description: Single Cell RNA


title: Pos Ctrl_R67_C9

description: Single Cell RNA


title: S_R1_C48

description: Single Cell RNA


title: Pos Ctrl_R58_C9

description: Single Cell RNA


title: Pos Ctrl_R67_C57

description: Single Cell RNA


title: Pos Ctrl_R67_C21

description: Single Cell RNA


title: Pos Ctrl_R67_C45

description: Single Cell RNA


title: Pos Ctrl_R58_C33

description: Single Cell RNA


title: Pos Ctrl_R58_C45

description: Single Cell RNA


title: Pos Ctrl_R58_C69

description: Single Cell RNA


title: Pos Ctrl_R58_C21

description: Single Cell RNA


title: Pos Ctrl_R49_C57

description: Single Cell RNA


title: Pos Ctrl_R49_C45

description: Single Cell RNA


title: Pos Ctrl_R49_C21

description: Single Cell RNA


title: S_R10_C5

description: Single Cell RNA


title: Pos Ctrl_R58_C57

description: Single Cell RNA


title: S_R0_C58

description: Single Cell RNA


title: S_R0_C9

description: Single Cell RNA


title: Pos Ctrl_R49_C9

description: Single Cell RNA


title: Pos Ctrl_R49_C69

description: Single Cell RNA


title: Pos Ctrl_R40_C33

description: Single Cell RNA


title: Pos Ctrl_R40_C21

description: Single Cell RNA


title: Pos Ctrl_R49_C33

description: Single Cell RNA


title: Pos Ctrl_R4_C69

description: Single Cell RNA


title: Pos Ctrl_R4_C45

description: Single Cell RNA


title: Pos Ctrl_R4_C33

description: Single Cell RNA


title: Pos Ctrl_R40_C9

description: Single Cell RNA


title: Pos Ctrl_R4_C21

description: Single Cell RNA


title: S_R0_C1

description: Single Cell RNA


title: Pos Ctrl_R40_C69

description: Single Cell RNA


title: Pos Ctrl_R40_C57

description: Single Cell RNA


title: Pos Ctrl_R31_C45

description: Single Cell RNA


title: Pos Ctrl_R31_C33

description: Single Cell RNA


title: Pos Ctrl_R67_C33

description: Single Cell RNA


title: Pos Ctrl_R22_C9

description: Single Cell RNA


title: Pos Ctrl_R4_C9

description: Single Cell RNA


title: Pos Ctrl_R22_C57

description: Single Cell RNA


title: Pos Ctrl_R22_C21

description: Single Cell RNA


title: Pos Ctrl_R31_C69

description: Single Cell RNA


title: Pos Ctrl_R13_C57

description: Single Cell RNA


title: Pos Ctrl_R31_C9

description: Single Cell RNA


title: Pos Ctrl_R31_C57

description: Single Cell RNA


title: Pos Ctrl_R13_C21

description: Single Cell RNA


title: Pos Ctrl_R31_C21

description: Single Cell RNA


title: Pos Ctrl_R22_C69

description: Single Cell RNA


title: Pos Ctrl_R13_C33

description: Single Cell RNA


title: Neg Ctrl_R67_C26

description: Single Cell RNA


title: Neg Ctrl_R67_C38

description: Single Cell RNA


title: Neg Ctrl_R58_C62

description: Single Cell RNA


title: Neg Ctrl_R67_C2

description: Single Cell RNA


title: Pos Ctrl_R13_C69

description: Single Cell RNA


title: Pos Ctrl_R22_C45

description: Single Cell RNA


title: Pos Ctrl_R40_C45

description: Single Cell RNA


title: Neg Ctrl_R58_C50

description: Single Cell RNA


title: Pos Ctrl_R4_C57

description: Single Cell RNA


title: Neg Ctrl_R58_C38

description: Single Cell RNA


title: Pos Ctrl_R13_C45

description: Single Cell RNA


title: Neg Ctrl_R67_C50

description: Single Cell RNA


title: Neg Ctrl_R58_C14

description: Single Cell RNA


title: Neg Ctrl_R58_C26

description: Single Cell RNA


title: Neg Ctrl_R58_C2

description: Single Cell RNA


title: Neg Ctrl_R67_C62

description: Single Cell RNA


title: Neg Ctrl_R49_C26

description: Single Cell RNA


title: Neg Ctrl_R49_C50

description: Single Cell RNA


title: Neg Ctrl_R49_C62

description: Single Cell RNA


title: Neg Ctrl_R49_C38

description: Single Cell RNA


title: Neg Ctrl_R49_C14

description: Single Cell RNA


title: Neg Ctrl_R40_C50

description: Single Cell RNA


title: Neg Ctrl_R49_C2

description: Single Cell RNA


title: Neg Ctrl_R40_C62

description: Single Cell RNA


title: Neg Ctrl_R67_C14

description: Single Cell RNA


title: Neg Ctrl_R40_C14

description: Single Cell RNA


title: Neg Ctrl_R4_C50

description: Single Cell RNA


title: Neg Ctrl_R40_C38

description: Single Cell RNA


title: Neg Ctrl_R40_C26

description: Single Cell RNA


title: Neg Ctrl_R4_C26

description: Single Cell RNA


title: Neg Ctrl_R4_C2

description: Single Cell RNA


title: Neg Ctrl_R31_C50

description: Single Cell RNA


title: Neg Ctrl_R4_C38

description: Single Cell RNA


title: Neg Ctrl_R4_C14

description: Single Cell RNA


title: Neg Ctrl_R31_C38

description: Single Cell RNA


title: Neg Ctrl_R31_C14

description: Single Cell RNA


title: Neg Ctrl_R31_C62

description: Single Cell RNA


title: Neg Ctrl_R31_C26

description: Single Cell RNA


title: Neg Ctrl_R22_C62

description: Single Cell RNA


title: Neg Ctrl_R22_C50

description: Single Cell RNA


title: Neg Ctrl_R31_C2

description: Single Cell RNA


title: Neg Ctrl_R22_C38

description: Single Cell RNA


title: Neg Ctrl_R22_C2

description: Single Cell RNA


title: Neg Ctrl_R22_C14

description: Single Cell RNA


title: Neg Ctrl_R13_C38

description: Single Cell RNA


title: Neg Ctrl_R13_C62

description: Single Cell RNA


title: Neg Ctrl_R13_C26

description: Single Cell RNA


title: Neg Ctrl_R22_C26

description: Single Cell RNA


title: Neg Ctrl_R40_C2

description: Single Cell RNA


title: Neg Ctrl_R13_C2

description: Single Cell RNA


title: Neg Ctrl_R13_C14

description: Single Cell RNA


title: Neg Ctrl_R4_C62

description: Single Cell RNA


title: S_R9_C6

description: Single Cell RNA


title: S_R9_C70

description: Single Cell RNA


title: S_R9_C55

description: Single Cell RNA


title: S_R9_C48

description: Single Cell RNA


title: S_R9_C33

description: Single Cell RNA


title: S_R9_C21

description: Single Cell RNA


title: S_R9_C5

description: Single Cell RNA


title: S_R9_C20

description: Single Cell RNA


title: Neg Ctrl_R13_C50

description: Single Cell RNA


title: S_R9_C0

description: Single Cell RNA


title: S_R9_C18

description: Single Cell RNA


title: S_R9_C26

description: Single Cell RNA


title: S_R8_C60

description: Single Cell RNA


title: S_R8_C66

description: Single Cell RNA


title: S_R8_C6

description: Single Cell RNA


title: S_R8_C69

description: Single Cell RNA


title: S_R8_C5

description: Single Cell RNA


title: S_R8_C44

description: Single Cell RNA


title: S_R8_C41

description: Single Cell RNA


title: S_R8_C43

description: Single Cell RNA


title: S_R8_C40

description: Single Cell RNA


title: S_R8_C35

description: Single Cell RNA


title: S_R8_C48

description: Single Cell RNA


title: S_R8_C30

description: Single Cell RNA


title: S_R8_C3

description: Single Cell RNA


title: S_R9_C56

description: Single Cell RNA


title: S_R8_C25

description: Single Cell RNA


title: S_R8_C27

description: Single Cell RNA


title: S_R8_C20

description: Single Cell RNA


title: S_R8_C70

description: Single Cell RNA


title: S_R71_C69

description: Single Cell RNA


title: S_R71_C64

description: Single Cell RNA


title: S_R8_C16

description: Single Cell RNA


title: S_R8_C19

description: Single Cell RNA


title: S_R71_C60

description: Single Cell RNA


title: S_R71_C6

description: Single Cell RNA


title: S_R71_C33

description: Single Cell RNA


title: S_R71_C28

description: Single Cell RNA


title: S_R71_C3

description: Single Cell RNA


title: S_R71_C36

description: Single Cell RNA


title: S_R8_C57

description: Single Cell RNA


title: S_R71_C19

description: Single Cell RNA


title: S_R71_C2

description: Single Cell RNA


title: S_R8_C29

description: Single Cell RNA


title: S_R71_C13

description: Single Cell RNA


title: S_R70_C7

description: Single Cell RNA


title: S_R70_C62

description: Single Cell RNA


title: S_R71_C53

description: Single Cell RNA


title: S_R71_C11

description: Single Cell RNA


title: S_R71_C62

description: Single Cell RNA


title: S_R70_C57

description: Single Cell RNA


title: S_R70_C54

description: Single Cell RNA


title: S_R71_C21

description: Single Cell RNA


title: S_R71_C12

description: Single Cell RNA


title: S_R70_C67

description: Single Cell RNA


title: S_R70_C44

description: Single Cell RNA


title: S_R8_C11

description: Single Cell RNA


title: S_R70_C46

description: Single Cell RNA


title: S_R70_C36

description: Single Cell RNA


title: S_R71_C39

description: Single Cell RNA


title: S_R70_C27

description: Single Cell RNA


title: S_R70_C31

description: Single Cell RNA


title: S_R70_C19

description: Single Cell RNA


title: S_R70_C12

description: Single Cell RNA


title: S_R70_C11

description: Single Cell RNA


title: S_R7_C68

description: Single Cell RNA


title: S_R7_C51

description: Single Cell RNA


title: S_R7_C67

description: Single Cell RNA


title: S_R7_C57

description: Single Cell RNA


title: S_R7_C56

description: Single Cell RNA


title: S_R7_C50

description: Single Cell RNA


title: S_R7_C44

description: Single Cell RNA


title: S_R7_C43

description: Single Cell RNA


title: S_R7_C5

description: Single Cell RNA


title: S_R70_C35

description: Single Cell RNA


title: S_R7_C42

description: Single Cell RNA


title: S_R7_C47

description: Single Cell RNA


title: S_R7_C39

description: Single Cell RNA


title: S_R70_C33

description: Single Cell RNA


title: S_R7_C23

description: Single Cell RNA


title: S_R7_C22

description: Single Cell RNA


title: S_R7_C21

description: Single Cell RNA


title: S_R7_C32

description: Single Cell RNA


title: S_R7_C30

description: Single Cell RNA


title: S_R7_C25

description: Single Cell RNA


title: S_R7_C9

description: Single Cell RNA


title: S_R7_C18

description: Single Cell RNA


title: S_R7_C14

description: Single Cell RNA


title: S_R7_C16

description: Single Cell RNA


title: S_R70_C10

description: Single Cell RNA


title: S_R7_C11

description: Single Cell RNA


title: S_R7_C13

description: Single Cell RNA


title: S_R7_C15

description: Single Cell RNA


title: S_R69_C69

description: Single Cell RNA


title: S_R69_C57

description: Single Cell RNA


title: S_R69_C63

description: Single Cell RNA


title: S_R7_C36

description: Single Cell RNA


title: S_R69_C54

description: Single Cell RNA


title: S_R69_C55

description: Single Cell RNA


title: S_R70_C71

description: Single Cell RNA


title: S_R69_C43

description: Single Cell RNA


title: S_R69_C36

description: Single Cell RNA


title: S_R69_C17

description: Single Cell RNA


title: S_R69_C30

description: Single Cell RNA


title: S_R69_C5

description: Single Cell RNA


title: S_R69_C22

description: Single Cell RNA


title: S_R69_C15

description: Single Cell RNA


title: S_R68_C57

description: Single Cell RNA


title: S_R68_C53

description: Single Cell RNA


title: S_R69_C50

description: Single Cell RNA


title: S_R68_C49

description: Single Cell RNA


title: S_R68_C51

description: Single Cell RNA


title: S_R68_C54

description: Single Cell RNA


title: S_R68_C63

description: Single Cell RNA


title: S_R68_C52

description: Single Cell RNA


title: S_R68_C35

description: Single Cell RNA


title: S_R68_C45

description: Single Cell RNA


title: S_R68_C44

description: Single Cell RNA


title: S_R68_C34

description: Single Cell RNA


title: S_R68_C32

description: Single Cell RNA


title: S_R69_C58

description: Single Cell RNA


title: S_R68_C31

description: Single Cell RNA


title: S_R68_C14

description: Single Cell RNA


title: S_R67_C68

description: Single Cell RNA


title: S_R67_C65

description: Single Cell RNA


title: S_R67_C67

description: Single Cell RNA


title: S_R68_C56

description: Single Cell RNA


title: S_R67_C32

description: Single Cell RNA


title: S_R67_C39

description: Single Cell RNA


title: S_R67_C6

description: Single Cell RNA


title: S_R68_C4

description: Single Cell RNA


title: S_R67_C25

description: Single Cell RNA


title: S_R67_C29

description: Single Cell RNA


title: S_R66_C67

description: Single Cell RNA


title: S_R67_C48

description: Single Cell RNA


title: S_R67_C20

description: Single Cell RNA


title: S_R67_C36

description: Single Cell RNA


title: S_R67_C17

description: Single Cell RNA


title: S_R66_C70

description: Single Cell RNA


title: S_R66_C59

description: Single Cell RNA


title: S_R66_C65

description: Single Cell RNA


title: S_R66_C56

description: Single Cell RNA


title: S_R66_C63

description: Single Cell RNA


title: S_R66_C54

description: Single Cell RNA


title: S_R68_C2

description: Single Cell RNA


title: S_R66_C45

description: Single Cell RNA


title: S_R66_C46

description: Single Cell RNA


title: S_R66_C6

description: Single Cell RNA


title: S_R67_C12

description: Single Cell RNA


title: S_R67_C22

description: Single Cell RNA


title: S_R66_C27

description: Single Cell RNA


title: S_R66_C4

description: Single Cell RNA


title: S_R66_C17

description: Single Cell RNA


title: S_R66_C24

description: Single Cell RNA


title: S_R66_C42

description: Single Cell RNA


title: S_R66_C29

description: Single Cell RNA


title: S_R66_C15

description: Single Cell RNA


title: S_R65_C71

description: Single Cell RNA


title: S_R65_C59

description: Single Cell RNA


title: S_R65_C60

description: Single Cell RNA


title: S_R65_C65

description: Single Cell RNA


title: S_R65_C53

description: Single Cell RNA


title: S_R65_C6

description: Single Cell RNA


title: S_R65_C68

description: Single Cell RNA


title: S_R65_C54

description: Single Cell RNA


title: S_R67_C27

description: Single Cell RNA


title: S_R67_C64

description: Single Cell RNA


title: S_R65_C70

description: Single Cell RNA


title: S_R65_C52

description: Single Cell RNA


title: S_R65_C39

description: Single Cell RNA


title: S_R65_C48

description: Single Cell RNA


title: S_R65_C41

description: Single Cell RNA


title: S_R65_C49

description: Single Cell RNA


title: S_R65_C32

description: Single Cell RNA


title: S_R65_C40

description: Single Cell RNA


title: S_R65_C21

description: Single Cell RNA


title: S_R65_C25

description: Single Cell RNA


title: S_R65_C17

description: Single Cell RNA


title: S_R65_C20

description: Single Cell RNA


title: S_R64_C71

description: Single Cell RNA


title: S_R64_C67

description: Single Cell RNA


title: S_R65_C11

description: Single Cell RNA


title: S_R64_C7

description: Single Cell RNA


title: S_R65_C12

description: Single Cell RNA


title: S_R64_C58

description: Single Cell RNA


title: S_R64_C66

description: Single Cell RNA


title: S_R64_C52

description: Single Cell RNA


title: S_R64_C42

description: Single Cell RNA


title: S_R64_C43

description: Single Cell RNA


title: S_R64_C51

description: Single Cell RNA


title: S_R64_C44

description: Single Cell RNA


title: S_R65_C29

description: Single Cell RNA


title: S_R64_C36

description: Single Cell RNA


title: S_R64_C33

description: Single Cell RNA


title: S_R64_C32

description: Single Cell RNA


title: S_R63_C61

description: Single Cell RNA


title: S_R64_C0

description: Single Cell RNA


title: S_R63_C8

description: Single Cell RNA


title: S_R63_C7

description: Single Cell RNA


title: S_R63_C63

description: Single Cell RNA


title: S_R63_C53

description: Single Cell RNA


title: S_R64_C57

description: Single Cell RNA


title: S_R63_C60

description: Single Cell RNA


title: S_R63_C43

description: Single Cell RNA


title: S_R63_C38

description: Single Cell RNA


title: S_R63_C46

description: Single Cell RNA


title: S_R64_C14

description: Single Cell RNA


title: S_R63_C58

description: Single Cell RNA


title: S_R63_C1

description: Single Cell RNA


title: S_R63_C21

description: Single Cell RNA


title: S_R62_C71

description: Single Cell RNA


title: S_R62_C9

description: Single Cell RNA


title: S_R63_C10

description: Single Cell RNA


title: S_R63_C15

description: Single Cell RNA


title: S_R63_C6

description: Single Cell RNA


title: S_R62_C70

description: Single Cell RNA


title: S_R63_C34

description: Single Cell RNA


title: S_R62_C51

description: Single Cell RNA


title: S_R62_C57

description: Single Cell RNA


title: S_R62_C50

description: Single Cell RNA


title: S_R62_C5

description: Single Cell RNA


title: S_R62_C60

description: Single Cell RNA


title: S_R64_C11

description: Single Cell RNA


title: S_R62_C49

description: Single Cell RNA


title: S_R62_C47

description: Single Cell RNA


title: S_R62_C46

description: Single Cell RNA


title: S_R62_C35

description: Single Cell RNA


title: S_R62_C25

description: Single Cell RNA


title: S_R62_C3

description: Single Cell RNA


title: S_R62_C44

description: Single Cell RNA


title: S_R62_C15

description: Single Cell RNA


title: S_R62_C10

description: Single Cell RNA


title: S_R61_C68

description: Single Cell RNA


title: S_R61_C63

description: Single Cell RNA


title: S_R61_C62

description: Single Cell RNA


title: S_R61_C64

description: Single Cell RNA


title: S_R61_C24

description: Single Cell RNA


title: S_R61_C22

description: Single Cell RNA


title: S_R61_C61

description: Single Cell RNA


title: S_R68_C43

description: Single Cell RNA


title: S_R61_C5

description: Single Cell RNA


title: S_R61_C29

description: Single Cell RNA


title: S_R61_C12

description: Single Cell RNA


title: S_R61_C28

description: Single Cell RNA


title: S_R61_C2

description: Single Cell RNA


title: S_R61_C27

description: Single Cell RNA


title: S_R61_C41

description: Single Cell RNA


title: S_R62_C11

description: Single Cell RNA


title: S_R60_C70

description: Single Cell RNA


title: S_R61_C60

description: Single Cell RNA


title: S_R60_C71

description: Single Cell RNA


title: S_R60_C65

description: Single Cell RNA


title: S_R62_C42

description: Single Cell RNA


title: S_R61_C56

description: Single Cell RNA


title: S_R60_C60

description: Single Cell RNA


title: S_R60_C57

description: Single Cell RNA


title: S_R60_C68

description: Single Cell RNA


title: S_R60_C47

description: Single Cell RNA


title: S_R60_C41

description: Single Cell RNA


title: S_R60_C59

description: Single Cell RNA


title: S_R60_C4

description: Single Cell RNA


title: S_R60_C49

description: Single Cell RNA


title: S_R60_C37

description: Single Cell RNA


title: S_R60_C38

description: Single Cell RNA


title: S_R60_C36

description: Single Cell RNA


title: S_R60_C32

description: Single Cell RNA


title: S_R61_C15

description: Single Cell RNA


title: S_R60_C3

description: Single Cell RNA


title: S_R60_C28

description: Single Cell RNA


title: S_R60_C26

description: Single Cell RNA


title: S_R60_C22

description: Single Cell RNA


title: S_R60_C17

description: Single Cell RNA


title: S_R60_C64

description: Single Cell RNA


title: S_R60_C20

description: Single Cell RNA


title: S_R60_C10

description: Single Cell RNA


title: S_R60_C1

description: Single Cell RNA


title: S_R6_C65

description: Single Cell RNA


title: S_R6_C9

description: Single Cell RNA


title: S_R6_C50

description: Single Cell RNA


title: S_R6_C49

description: Single Cell RNA


title: S_R60_C46

description: Single Cell RNA


title: S_R6_C3

description: Single Cell RNA


title: S_R6_C51

description: Single Cell RNA


title: S_R6_C31

description: Single Cell RNA


title: S_R60_C6

description: Single Cell RNA


title: S_R6_C28

description: Single Cell RNA


title: S_R6_C27

description: Single Cell RNA


title: S_R6_C48

description: Single Cell RNA


title: S_R6_C26

description: Single Cell RNA


title: S_R6_C24

description: Single Cell RNA


title: S_R6_C11

description: Single Cell RNA


title: S_R6_C23

description: Single Cell RNA


title: S_R6_C21

description: Single Cell RNA


title: S_R59_C70

description: Single Cell RNA


title: S_R60_C19

description: Single Cell RNA


title: S_R59_C53

description: Single Cell RNA


title: S_R59_C51

description: Single Cell RNA


title: S_R59_C63

description: Single Cell RNA


title: S_R59_C5

description: Single Cell RNA


title: S_R59_C46

description: Single Cell RNA


title: S_R59_C49

description: Single Cell RNA


title: S_R59_C4

description: Single Cell RNA


title: S_R59_C40

description: Single Cell RNA


title: S_R59_C48

description: Single Cell RNA


title: S_R59_C38

description: Single Cell RNA


title: S_R59_C37

description: Single Cell RNA


title: S_R59_C25

description: Single Cell RNA


title: S_R59_C22

description: Single Cell RNA


title: S_R59_C19

description: Single Cell RNA


title: S_R59_C17

description: Single Cell RNA


title: S_R59_C11

description: Single Cell RNA


title: S_R59_C16

description: Single Cell RNA


title: S_R59_C3

description: Single Cell RNA


title: S_R59_C14

description: Single Cell RNA


title: S_R58_C65

description: Single Cell RNA


title: S_R58_C7

description: Single Cell RNA


title: S_R58_C59

description: Single Cell RNA


title: S_R59_C71

description: Single Cell RNA


title: S_R58_C53

description: Single Cell RNA


title: S_R58_C48

description: Single Cell RNA


title: S_R58_C51

description: Single Cell RNA


title: S_R58_C4

description: Single Cell RNA


title: S_R58_C47

description: Single Cell RNA


title: S_R58_C36

description: Single Cell RNA


title: S_R58_C28

description: Single Cell RNA


title: S_R58_C20

description: Single Cell RNA


title: S_R58_C44

description: Single Cell RNA


title: S_R58_C18

description: Single Cell RNA


title: S_R57_C61

description: Single Cell RNA


title: S_R58_C12

description: Single Cell RNA


title: S_R59_C23

description: Single Cell RNA


title: S_R57_C70

description: Single Cell RNA


title: S_R57_C54

description: Single Cell RNA


title: S_R57_C55

description: Single Cell RNA


title: S_R57_C51

description: Single Cell RNA


title: S_R57_C40

description: Single Cell RNA


title: S_R57_C38

description: Single Cell RNA


title: S_R57_C34

description: Single Cell RNA


title: S_R57_C32

description: Single Cell RNA


title: S_R57_C33

description: Single Cell RNA


title: S_R57_C3

description: Single Cell RNA


title: S_R57_C29

description: Single Cell RNA


title: S_R57_C27

description: Single Cell RNA


title: S_R58_C19

description: Single Cell RNA


title: NUP98-HBO1

city: Hachioji

first: Yoshihiro

last: Hayashi

state: Tokyo

address: 1432-1 Horinouchi

country: Japan

institute: Tokyo University of Pharmacy and Life Sciences

hyb_protocol: We followed manufacturer's protocol.

scan_protocol: We followed manufacturer's protocol, and used GenePix 4000B(Molecular Devices, Inc).

data_processing: CodeLink Expression Analysis v5.0 software, Quantile   normalization

source_name: Cord Blood CD34+ cells

label_protocol: We followed manufacturer's protocol.

extract_protocol: Total RNA was extracted from BM cells using Trizol (Invitrogen, Carlsbad, CA).

tag: transduced

value: Cord Blood CD34+ cells


title: Control

value: empty vector


title: S_R57_C56

description: Single Cell RNA


title: HEK293T

city: beijing

first: wanru

last: ma

address: 52 Fucheng Road,Haidian District,Beijing

country: China

institute: beijing cancer hospital

data_processing:
Illumina sequencing adapters and low quality reads (<40bp) in the raw fastq data were filtered by cutadapt.
Reads mapping was performed using TopHat.
Gene expression was quantified using htseq.
Genome_build: hg19 (GRCh37)
Supplementary_files_format_and_content: *.htseqcount: Tab-delimited text files include htseq count for each Sample

source_name: human embryonic kidney cell

extract_protocol:
RNA was extracted through Trizol Reagent.
QIAseq Stranded RNA Library Kits (180745, Qiagen).

value: embryonic kidney cell line


title: MCF7

source_name: human breast adenocarcinoma cell line

value: breast adenocarcinoma cell line


title: HMC-1.2_NM2_4

city: marseille

first: Amira

last: Ben Amara

address: 27  Boulevard Leï Roue

country: France

institute: IHU-CRCM

description: Purified cells

hyb_protocol: RNA was analyzed using microarray chips including 45,000 probes (4x44K Whole Human Genome, Agilent Technologies) and One-color Microarray Based Gene Expression Analysis. Hybridization was performed at 65°C using the in situ Hybridization Plus kit (Agilent Technologies) for 17 hours.

scan_protocol: Arrays were scanned with a pixel size of 5 µm with the DNA Microarray Scanner  G2505C. Image analysis and correction of intra-array signals were performed with the Feature Extraction Software10.7.3.1 (Agilent Technologies).

data_processing: Microarray data analysis was performed using the GeneSpring software. Raw data were filtered and normalized, unsupervised and supervised analyses were done using hierarchical clustering, principal composant analysis (PCA) and the signicantly modulated genes using GeneSpring Software (Agilent Technologies). Genes were considered to be differentially expressed when fold change (FC) was  FC ≥ 2 with p-value (p) was p < 0.001 in at least one of the 3 pair combinations. Gene Ontology (GO) annotations attributed to biological processes and corrected p ≤ 0.001 (Bonferroni correction) allow us to identify the biological functions that were modulated,

source_name: cell line MHC-1.2

label_protocol: In brief, 300 ng RNA were labeled with cyanine-3-CTP using the Low RNA Input Fluorescent Amplification Kit from Agilent Technologies.

growth_protocol: The human mast cell line HMC-1.2 was cultured in Iscove's Modified Dulbecco's Medium (IMDM, Life Technologies) supplemented with 10% fetal bovine serum (FBS), 100 IU/ml penicillin and 50 µg/ml streptomycin (Life Technologies) at 37°C.

extract_protocol: RNA was extracted using an RNeasy Mini kit (Qiagen, Courtaboeuf, France). The quantity and quality of total RNA were assessed using the Nanodrop (Thermo Scientific, Illkirch, France) and a 2100 Bioanalyzer (Agilent Technologies, Massy, France). The RNA was eluted in 30 µl of water and stored at -80°C.

treatment_protocol: Cells were cultured at 37°C for 8 hours with or without NM1or NM2 before processing RNAs extraction.

tag: condition

value: C. burnetii phase 2  non-pathogenic phase


title: HMC-1.2_NM2_2

description: Purified cells


title: HMC-1.2_NM1_4

description: Purified cells

value: C. burnetii phase 1  pathogenic phase


title: M418

city: Granada

first: Maria

middle: A.

last: Llamas

state: Andalusia/Granada

address: Profesor Albareda, 1. E-18008.

department: Environmental Protection. Environmental Microbiology and Biodegradation

country: Spain

institute: Estación Experimental del Zaidín (CSIC)

data_processing:
Samples were processed on the Illumina NextSeq500 Sequencer in one run with a read length of 2 × 75 bp.
The pipeline recommended in the tool Cufflinks 2.2.1 was used to analyze the data and obtain the set of differentially expressed genes following these steps: 1) Raw reads quality analysis  with the tool FastQC (http://www.bioinformatics.babraham.ac.uk/projects/fastqc); 2) Reads mapping to the P. aeruginosa PAO1 reference genome (NCBI reference sequence NC_002516.2) with the tool Bowtie  integrated in the Tophat suite, and 3) analysis of differences in gene expression with Cufflinks 2.2.1.
Genome_build: P. aeruginosa PAO1 reference genome (NCBI reference sequence NC_002516.2)
Supplementary_files_format_and_content: File Tab in Microsoft Excel of the gene track ('BACTRNA_Differential_expression_M40_M418_Deliverable') . This Excel has the following sheets: 1) M40-M18.geneexp.diff: this sheet contains the results of the comparison of the expression of ALL the genes reported in the reference genome. Sometimes there are no enough data to estimate the gene expression properly or there are too many. Only those genes whose status is ‘OK’ have been used to finally analyze the differences in their expression levels. 2)significant diff express.genes: List of differentially expressed genes with rich functional annotations obtained from Uniprot. underexpressed in sample2 (M418) are in red and overrexpressed in sample2 (M418) are in blue. 3)PAO1 annot gff diff exp genes: in this sheet you can find the genes differentially expressed in the gff annotation context.

organism: Pseudomonas aeruginosa

source_name: ΔvreR mutant

growth_protocol: Bacteria were grown until late exponential phase in low Pi medium.

extract_protocol:
Total RNA was extracted by the hot phenol method using the TRI® Reagent protocol (Ambion) and subjected to two DNase I treatments with the Turbo DNA-free kit (Ambion) and RNaseOUT (Invitrogen). RNA quality, including purity, integrity and yield, was assessed by electrophoresis of 1 µg of total RNA and by UV absorption at 260 nm in a ND-1000 spectrophotometer (NanoDrop Technologies, USA). The rRNA from 4 µg of total RNA was removed using the Ribo-Zero rRNA removal kit (Illumina) following the manufactures’ recommendations.
Sequencing libraries were prepared with the NEBNext Ultra directional RNA library Prep kit (New England Biolabs). RNA was fragmented at 94 °C for 7.5 min and first strand cDNA synthesis was performed at 42 °C during 50 min using the adaptor and primers recommended by the manufacturer (NEBNext Multiplex Oligos, Illumina).

tag: genotype/variation

value: deltavreR mutant


title: M40

source_name: Wild type

value: Wild type


title: Danio_rerio_RNAseq_singleHeart_3dpf_mut_44

city: Nijmegen

first: Leonie

middle: M

last: Kamminga

address: Geert Grooteplein 28

department: Molecular Biology

country: Netherlands

institute: Radboud University

description:
RNAseq of dissected hearts from 3 days post fertilization Danio rerio rnf2 KO embryos
singleHearts_monocle_3dpf_2sd_expressedGenes_normCounts.txt
NDCII_1_44_NDCII_sample_0044

data_processing:
RNA-seq reads from whole embryos were mapped to genome GRCz10 + transcriptome v87 from ensembl using STAR version 2.5.2b with --quantMode geneCounts
Batch effect was removed with R package RUVseq version 1.10.0 obtaining final normalized counts with DESeq2 version 1.16.1
ChIPseq reads were aligned to the genome GRCz10 using bwa version 0.7.15 with default parameters
Aligned reads were further processed removing multimappers and duplicated reads using Picard MarkDuplicates 2.8.2
ChIP-seq peaks were called using macs2 version 2.1.1 with qvalue cutoff=1e-02 relative to respective ChIP input track.
RNAseq reads from dissected hearts were demultiplexed and processed following the CEL-Seq pipeline (https://github.com/yanailab/CEL-Seq-pipeline)
Normalized counts were obtained with Monocle version 2.4.0
Genome_build: GRCz10
Supplementary_files_format_and_content: Gene counts (.txt); peak files in BED format

molecule: polyA RNA

source_name: heart

growth_protocol: Zebrafish (Danio rerio), were housed at 27.5°C in a 14/10h light/dark cycle. The evening before spawning, one male and one female were placed into a tank with a divider and merged the following morning. Spontaneous spawning occurred when the male and female were put together at the moment the light turned on. Embryos were collected and staged according to Kimmel et al. (Kimmel et al., 1995).

extract_protocol:
RNA-seq: Embryos of 3 dpf were homogenized in TRIzol and the ZYMO RNA microprep kit was used to isolate RNA and treat the samples with DNAseI. rRNA was depleted using the illumina RiboZero kit
RNA-seq: extraction was followed by fragmentation, cDNA synthesis, and KAPA-HYPERprep library preparation

value: TU/TLF mixed background

value: heart

tag: genotype


title: HMC-1.2_NM1_3

description: Purified cells


title: 24 hrs after the loading

city: TOKYO

first: Taku

last: Saito

address: 7-3-1 Hongo, Bunkyo-ku

department: Orthopaedic Surgery, Faculty of Medicine

institute: The University of Tokyo

hyb_protocol: 0.6ug of Cy3-labelled cRNA was fragmented at 60°C for 30 minutes in a reaction volume of 25 ul containing 1x Agilent fragmentation buffer and 2x Agilent blocking agent following the manufacturer’s instructions. On completion of the fragmentation reaction, 25 ul of 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to SurePrint G3 Mouse GE 8x60K Microarray (Agilent) for 17 hours at 65°C in a rotating Agilent hybridization oven.  After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent) and 1 minute with 37°C GE Wash buffer 2 (Agilent), then dried immediately by brief centrifugation.

scan_protocol: Slides were scanned immediately after washing on the the Agilent DNA Microarray Scanner (G2565CA) using one color scan setting for 8x60k array slides (Scan Area 61x21.6 mm, Scan resolution 3um, Dye channel is set to Green PMT is set to 100%).

data_processing: The scanned images were analyzed with Feature Extraction Software 10.10.1.1 (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities.

source_name: Primary chondrocytes subjected to mechanical loading

label_protocol: Cyanine-3 (Cy3) labeled cRNA was prepared from 0.1ug Total RNA using the Low Input Quick Amp Labeling Kit(Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA).  Dye incorporation and cRNA yield were checked with the NanoDrop ND-2000 Spectrophotometer.

extract_protocol: Total RNA was extracted from the chondrocytes using RNeasy mini kits (Qiagen) according to manufacturer’s protocol.

treatment_protocol: Mouse primary chondrocytes were seeded in silicon stretch chambers coated with fibronectin at a concentration of 1×10e5 cells/chamber. After 48 hours, cyclic tensile strain (0.5 Hz, 10% elongation) was applied for 30 minutes using STB-140 mechanical stretch system.

value: Primary chondrocytes


title: Danio_rerio_RNAseq_singleHeart_3dpf_mut_43

description:
RNAseq of dissected hearts from 3 days post fertilization Danio rerio rnf2 KO embryos
singleHearts_monocle_3dpf_2sd_expressedGenes_normCounts.txt
NDCII_1_43_NDCII_sample_0043


title: HMC-1.2_NM1_2

description: Purified cells


title: HMC-1.2_US_3

description: Purified cells

value: untreated