title: K562_H3k27me3_20181025_icell8_478_(20181025_Pool2_CATCGACC_TACTGACC)

description: Single-cell CUT&Tag for H3K27me3 in K562 cells using ICELL8 platform

extract_protocol:
Single-cell CUT&Tag: Approximately 1 million exponentially growing K562 cells were processed by centrifugation between buffer and reagent exchanges in low-retention tubes throughout. Centrifugations were performed at 600g for 3 min in a swinging bucket rotor for the initial wash and incubation steps, and then at 300g for 3 min after pA-Tn5 binding.  Cells were collected and washed twice with 1ml Wash Buffer (20 mM HEPES, pH 7.5; 150 mM NaCl; 0.5 mM Spermidine, 1X Protease inhibitor cocktail) at room temperature. Nuclei were isolated by permeabilizing cells in NP40-Digitonin Wash Buffer (0.01% NP40, 0.01% Digitonin in wash buffer) and resuspended in 0.25 mL of NP40-Digitonin Wash buffer with 1mM EDTA. Antibody was added at a 1:50 dilution and incubated on a rotator at 4°C overnight. Permeabilized cells were then rinsed once with NP40-Digitonin Wash buffer and incubated with anti-Rabbit IgG antibody (1:50 dilution) in 0.5 ml of NP40-Digitonin Wash buffer on a rotator at room temperature for 30 min. Nuclei were then washed 3x for 5 min in 1 ml NP40-Digitonin Wash buffer to remove unbound antibodies. For pA-Tn5 binding, a 1:200 dilution of pA-Tn5 adapter complex was prepared in 0.3 mL NP40-Dig-med-buffer (0.01% NP40, 0.01% Digitonin, 20 mM HEPES, pH 7.5, 300 mM NaCl, 0.5 mM Spermidine, 1X Protease inhibitor cocktail), and permeabilized cells were incubated with the pA-Tn5 adapter complex on a rotator at RT for 1 hr. Cells were washed 3x for 5 min in 1 ml NP40-Dig-med-buffer to remove excess pA-Tn5 protein. Cells were resuspended in 150 µL Tagmentation buffer (10 mM MgCl2 in NP40-Dig-med-buffer) and incubated at 37 for 1h. Tagmentation was stopped by adding 50 µL of 4X Stop Buffer (40.4 mM EDTA and 2 mg/ml DAPI) and the sample was held on ice for 30 minutes. The SMARTer ICELL8 single-cell system (Takara Bio USA, Cat. #640000) was used to array single cells as described for scATAC-seq12. DAPI-stained nuclei were visualized under the microscope and if there were clumps, they were strained through 10 micron cell strainers. Cells were counted using a hematocytometer and diluted at 28 cells/µL in 0.5X PBS and 1X Second Diluent (Takara Bio USA, Cat. # 640196). 10000 cells were loaded to the remaining 4 wells of the source loading plate. Control wells containing 0.5X PBS (25 µl) and fiducial mix (25 µl) (Takara Bio USA, Cat.  #640196) were also included in the source loading plate. Using the ICELL8 MultiSample NanoDispenser (MSND) FLA program, cells were dispensed into a SMARTer ICELL8 250v chip (Takara Bio USA, Cat. # 640183) at 35 nanoliter per well. After cell dispense was complete, chips were sealed with the imaging film (Takara Bio USA, Cat. #640109) and centrifuged at 400 xg for 5 min at room temperature and imaged using the ICELL8 imaging station (Takara Bio USA). Images were analyzed using automated microscopy image analysis software (CellSelect, Takara Bio USA). Since cells were stained only with DAPI, they were propidium iodide negative, so that permeabilized cells would not be excluded by default software settings. Additional single cells were manually selected for dispensing using a manual triaging procedure (total 479 single cells). Immediately following imaging, the filter file, which notes single-cell containing wells and control wells, was generated. All of the following reagents were added to the selected set of wells which contained single cells. To index the whole chip, 72 i5 and 72 i7 unique indices [Buenostro, J.D. et al. Single-cell chromatin accessibility reveals principles of regulatory variation. Nature 523, 486-U264 (2015)] were dispensed at 7.32 µM using ICELL8 MSND FLA program using the index 1 and index 2 filtered dispense tool respectively at 35 nanoliter per well. KAPA PCR mix (168 ul 5X HiFi Buffer + 25.2 ul 10mM dNTPs + 8.4 ul KAPA HiFi Polymerase non-hot start + 8.4 ul NF-water) was dispensed to selected wells using ICELL8 MSND FLA program at 35 nanoliter per well. The chip was sealed and centrifuged at 2250 xg at 4◦C for at least 5 min after each dispense. The chip was sealed with a TE Sealing film (Takara Bio USA, Cat. #640109) and on-chip PCR was performed using a SMARTer ICELL8 Thermal Cycler (Takara Bio USA) as follows: 5 min at 72 ◦C and 1 min at 98 ◦C followed by 15 cycles of 30 sec at 98 ◦C, 5 sec at 55 ◦C, with a final extension at 72 ◦C for 1 min. PCR products were collected by centrifugation at ~2250 xg for 20 min using the supplied SMARTer ICELL8 Collection Kit (Takara Bio USA, Cat.#640048). Pooled libraries were purified using Ampure XP beads (Beckman Counter) in a 1:1.1 ratio. Briefly, libraries were incubated with beads for 15 min at RT, washed twice in 80% ethanol, and eluted in 10 mM Tris pH 8.0. Paired-end 25x8x8x25 bp Illumina sequencing was performed on the pooled barcoded libraries following the manufacturer’s instructions.
Single-cell CUT&Tag

value: human immortalised myelogenous leukemia cell line


title: K562_H3k27me3_20181025_icell8_475_(20181025_Pool2_GAGCACGT_TCACATGG)

status: Public on Apr 29 2019

type: SRA

city: Seattle

first: Jorja

last: Henikoff

state: WA

address: 1100 Fairview AV N, A1-162

department: Basic Sciences

country: USA

institute: Fred Hutchinson Cancer Research Center

library_source: genomic

data_processing:
Genome_build: UCSC hg19
1. We used Bowtie2 2.2.5 with options "--end-to-end --very-sensitive --no-mixed --no-discordant --phred33 -I 10 -X 700" to map the 25bp paired-end Illumina reads to UCSC hg19. 2. We extracted fragments from properly paired reads (Supplementary file(s) .bed) .

molecule: genomic DNA

organism: Homo sapiens

source_name: Single cell CUT&Tag

tag: antibody

value: H3K27me3 Cell Signalling #9733

tag: cell line

tag: cell type


title: H1_H3K27me3_20181025_icell8_1_(20181025_Pool2_GGACTCCT_GCATTAAG)

description: Single-cell CUT&Tag for H3K27me3 in H1 cells using ICELL8 platform

extract_protocol:
Single-cell CUT&Tag: Approximately 1 million exponentially growing H1 cells were processed by centrifugation between buffer and reagent exchanges in low-retention tubes throughout. Centrifugations were performed at 600g for 3 min in a swinging bucket rotor for the initial wash and incubation steps, and then at 300g for 3 min after pA-Tn5 binding.  Cells were collected and washed twice with 1ml Wash Buffer (20 mM HEPES, pH 7.5; 150 mM NaCl; 0.5 mM Spermidine, 1X Protease inhibitor cocktail) at room temperature. Nuclei were isolated by permeabilizing cells in NP40-Digitonin Wash Buffer (0.01% NP40, 0.01% Digitonin in wash buffer) and resuspended in 0.25 mL of NP40-Digitonin Wash buffer with 1mM EDTA. Antibody was added at a 1:50 dilution and incubated on a rotator at 4°C overnight. Permeabilized cells were then rinsed once with NP40-Digitonin Wash buffer and incubated with anti-Rabbit IgG antibody (1:50 dilution) in 0.5 ml of NP40-Digitonin Wash buffer on a rotator at room temperature for 30 min. Nuclei were then washed 3x for 5 min in 1 ml NP40-Digitonin Wash buffer to remove unbound antibodies. For pA-Tn5 binding, a 1:200 dilution of pA-Tn5 adapter complex was prepared in 0.3 mL NP40-Dig-med-buffer (0.01% NP40, 0.01% Digitonin, 20 mM HEPES, pH 7.5, 300 mM NaCl, 0.5 mM Spermidine, 1X Protease inhibitor cocktail), and permeabilized cells were incubated with the pA-Tn5 adapter complex on a rotator at RT for 1 hr. Cells were washed 3x for 5 min in 1 ml NP40-Dig-med-buffer to remove excess pA-Tn5 protein. Cells were resuspended in 150 µL Tagmentation buffer (10 mM MgCl2 in NP40-Dig-med-buffer) and incubated at 37 for 1h. Tagmentation was stopped by adding 50 µL of 4X Stop Buffer (40.4 mM EDTA and 2 mg/ml DAPI) and the sample was held on ice for 30 minutes. The SMARTer ICELL8 single-cell system (Takara Bio USA, Cat. #640000) was used to array single cells as described for scATAC-seq12. DAPI-stained nuclei were visualized under the microscope and if there were clumps, they were strained through 10 micron cell strainers. Cells were counted using a hematocytometer and diluted at 28 cells/µL in 0.5X PBS and 1X Second Diluent (Takara Bio USA, Cat. # 640196). 10000 cells were loaded to 4 wells of the source loading plate. Control wells containing 0.5X PBS (25 µl) and fiducial mix (25 µl) (Takara Bio USA, Cat.  #640196) were also included in the source loading plate. Using the ICELL8 MultiSample NanoDispenser (MSND) FLA program, cells were dispensed into a SMARTer ICELL8 250v chip (Takara Bio USA, Cat. # 640183) at 35 nanoliter per well. After cell dispense was complete, chips were sealed with the imaging film (Takara Bio USA, Cat. #640109) and centrifuged at 400 xg for 5 min at room temperature and imaged using the ICELL8 imaging station (Takara Bio USA). Images were analyzed using automated microscopy image analysis software (CellSelect, Takara Bio USA). Since cells were stained only with DAPI, they were propidium iodide negative, so that permeabilized cells would not be excluded by default software settings. Additional single cells were manually selected for dispensing using a manual triaging procedure (~486 single cells in total). Immediately following imaging, the filter file, which notes single-cell containing wells and control wells, was generated. All of the following reagents were added to the selected set of wells which contained single cells. To index the whole chip, 72 i5 and 72 i7 unique indices [Buenostro, J.D. et al. Single-cell chromatin accessibility reveals principles of regulatory variation. Nature 523, 486-U264 (2015)] were dispensed at 7.32 µM using ICELL8 MSND FLA program using the index 1 and index 2 filtered dispense tool respectively at 35 nanoliter per well. KAPA PCR mix (168 ul 5X HiFi Buffer + 25.2 ul 10mM dNTPs + 8.4 ul KAPA HiFi Polymerase non-hot start + 8.4 ul NF-water) was dispensed to selected wells using ICELL8 MSND FLA program at 35 nanoliter per well. The chip was sealed and centrifuged at 2250 xg at 4◦C for at least 5 min after each dispense. The chip was sealed with a TE Sealing film (Takara Bio USA, Cat. #640109) and on-chip PCR was performed using a SMARTer ICELL8 Thermal Cycler (Takara Bio USA) as follows: 5 min at 72 ◦C and 1 min at 98 ◦C followed by 15 cycles of 30 sec at 98 ◦C, 5 sec at 55 ◦C, with a final extension at 72 ◦C for 1 min. PCR products were collected by centrifugation at ~2250 xg for 20 min using the supplied SMARTer ICELL8 Collection Kit (Takara Bio USA, Cat.#640048). Pooled libraries were purified using Ampure XP beads (Beckman Counter) in a 1:1.1 ratio. Briefly, libraries were incubated with beads for 15 min at RT, washed twice in 80% ethanol, and eluted in 10 mM Tris pH 8.0. Paired-end 25x8x8x25 bp Illumina sequencing was performed on the pooled barcoded libraries following the manufacturer’s instructions.
Single-cell CUT&Tag

value: human embryonic stem cells


title: H1_H3K27me3_20181025_icell8_2_(20181025_Pool2_GTTAACAG_GGCACGGA)


title: K562_H3k27me3_20181025_icell8_473_(20181025_Pool2_CCTCGCAG_AAGGAGTA)


title: K562_H3k27me3_20181025_icell8_477_(20181025_Pool2_AAGAGGCA_GTAAGGAG)


title: K562_H3k27me3_20181025_icell8_474_(20181025_Pool2_CTCATGGG_TTTCTAGC)


title: K562_H3k27me3_20181025_icell8_479_(20181025_Pool2_AATTCGTT_TTAACTTG)


title: K562_H3k27me3_20181025_icell8_471_(20181025_Pool2_ATGGGAAC_ACCGGCCC)


title: K562_H3k27me3_20181025_icell8_470_(20181025_Pool2_CCAACACG_TTGCCTAA)


title: K562_H3k27me3_20181025_icell8_466_(20181025_Pool2_TCAGCTAT_TGCATACT)


title: K562_H3k27me3_20181025_icell8_469_(20181025_Pool2_CATCGACC_TCAAGAGC)


title: K562_H3k27me3_20181025_icell8_468_(20181025_Pool2_AGGCAGAA_AGGTTGCC)


title: K562_H3k27me3_20181025_icell8_467_(20181025_Pool2_AACGCCAT_TGGGCGGT)


title: H1_H3K27me3_20181025_icell8_5_(20181025_Pool2_TACACCTC_GGCACGGA)


title: H1_H3K27me3_20181025_icell8_6_(20181025_Pool2_GTTAACAG_GCATTAAG)


title: K562_H3k27me3_20181025_icell8_472_(20181025_Pool2_AGGCAGAA_ATCCAGGA)


title: K562_H3k27me3_20181025_icell8_476_(20181025_Pool2_GTGATAGC_GGTTCAAT)


title: K562_H3k27me3_20181025_icell8_465_(20181025_Pool2_CCTCGCAG_GTGTATCG)


title: K562_H3k27me3_20181025_icell8_464_(20181025_Pool2_AGACCAGC_ATTGGGTC)


title: K562_H3k27me3_20181025_icell8_461_(20181025_Pool2_ATAAGTTA_ACTTGTTG)


title: K562_H3k27me3_20181025_icell8_456_(20181025_Pool2_AGACCAGC_TTGCCTAA)


title: K562_H3k27me3_20181025_icell8_463_(20181025_Pool2_AAGAGGCA_AGTCCGAG)


title: K562_H3k27me3_20181025_icell8_452_(20181025_Pool2_CTCTCTAC_TGGAAATC)


title: K562_H3k27me3_20181025_icell8_462_(20181025_Pool2_AAGAGGCA_AACATGAT)


title: K562_H3k27me3_20181025_icell8_460_(20181025_Pool2_TGGTCACA_TGTTCCTG)


title: K562_H3k27me3_20181025_icell8_451_(20181025_Pool2_AGGCAGAA_TCACATGG)


title: K562_H3k27me3_20181025_icell8_455_(20181025_Pool2_TTGACCCT_GGTGAAGG)


title: K562_H3k27me3_20181025_icell8_449_(20181025_Pool2_TCAGCTAT_CCTGCGGG)


title: K562_H3k27me3_20181025_icell8_448_(20181025_Pool2_AGGCAGAA_TCTGGCGA)


title: K562_H3k27me3_20181025_icell8_453_(20181025_Pool2_AGGCAGAA_AATCTACA)


title: K562_H3k27me3_20181025_icell8_457_(20181025_Pool2_AAGAGGCA_TACTGACC)


title: K562_H3k27me3_20181025_icell8_454_(20181025_Pool2_CAATCTTA_TACTGACC)


title: K562_H3k27me3_20181025_icell8_447_(20181025_Pool2_GTATTCGG_AGTCCGAG)


title: K562_H3k27me3_20181025_icell8_444_(20181025_Pool2_AAATACAG_CCTTATGT)


title: K562_H3k27me3_20181025_icell8_446_(20181025_Pool2_ATGGGAAC_CCTGCGGG)


title: K562_H3k27me3_20181025_icell8_459_(20181025_Pool2_AAATACAG_ATCCAGGA)


title: K562_H3k27me3_20181025_icell8_441_(20181025_Pool2_CCAACACG_CTAAGCCT)


title: K562_H3k27me3_20181025_icell8_440_(20181025_Pool2_CTCTCTAC_ATCCAGGA)


title: K562_H3k27me3_20181025_icell8_445_(20181025_Pool2_TGGTCACA_GTAAGGAG)


title: K562_H3k27me3_20181025_icell8_443_(20181025_Pool2_AAGAGGCA_AGAGATCT)


title: K562_H3k27me3_20181025_icell8_442_(20181025_Pool2_CAGAGAGG_GGGCTTGG)


title: K562_H3k27me3_20181025_icell8_438_(20181025_Pool2_CCTCGCAG_ACTTAGAA)


title: K562_H3k27me3_20181025_icell8_458_(20181025_Pool2_ATAAGTTA_ATCCAGGA)


title: K562_H3k27me3_20181025_icell8_439_(20181025_Pool2_CCTCGCAG_TCTGGCGA)


title: K562_H3k27me3_20181025_icell8_436_(20181025_Pool2_TCCTGAGC_CGTAGACC)


title: K562_H3k27me3_20181025_icell8_437_(20181025_Pool2_TTGACCCT_ATCCAGGA)


title: K562_H3k27me3_20181025_icell8_435_(20181025_Pool2_TCCTGAGC_AGGTTGCC)


title: K562_H3k27me3_20181025_icell8_450_(20181025_Pool2_TCAGCTAT_GCCCACGT)


title: K562_H3k27me3_20181025_icell8_432_(20181025_Pool2_TTTAATGC_CCTGCGGG)


title: K562_H3k27me3_20181025_icell8_434_(20181025_Pool2_GTAGAGGA_GTGTATCG)


title: K562_H3k27me3_20181025_icell8_426_(20181025_Pool2_GGCGTCGA_ACTGCATA)


title: K562_H3k27me3_20181025_icell8_422_(20181025_Pool2_TTCGCGGC_ACCGGCCC)


title: K562_H3k27me3_20181025_icell8_428_(20181025_Pool2_TTGACCCT_AGAGTAGA)


title: K562_H3k27me3_20181025_icell8_429_(20181025_Pool2_GACGCTCC_GGTGAAGG)


title: K562_H3k27me3_20181025_icell8_421_(20181025_Pool2_AAATACAG_TCAAGAGC)


title: K562_H3k27me3_20181025_icell8_427_(20181025_Pool2_TCCTGAGC_ACGCGCAG)


title: K562_H3k27me3_20181025_icell8_433_(20181025_Pool2_CTCTCTAC_CGGACTGC)


title: K562_H3k27me3_20181025_icell8_423_(20181025_Pool2_TCCTGAGC_TTCGTGCA)


title: K562_H3k27me3_20181025_icell8_420_(20181025_Pool2_ATAAGTTA_GGCACGGA)


title: K562_H3k27me3_20181025_icell8_430_(20181025_Pool2_GTGATAGC_ACGCGCAG)


title: K562_H3k27me3_20181025_icell8_425_(20181025_Pool2_GTGATAGC_CAGCAACG)


title: K562_H3k27me3_20181025_icell8_413_(20181025_Pool2_ATGGGAAC_ACTTGTTG)


title: K562_H3k27me3_20181025_icell8_417_(20181025_Pool2_GACGCTCC_AGAGATCT)


title: K562_H3k27me3_20181025_icell8_419_(20181025_Pool2_AGGCAGAA_ACGATAAC)


title: K562_H3k27me3_20181025_icell8_416_(20181025_Pool2_GTAGAGGA_ATGGTCAT)


title: K562_H3k27me3_20181025_icell8_414_(20181025_Pool2_GTATTCGG_GATTCCCA)


title: K562_H3k27me3_20181025_icell8_409_(20181025_Pool2_CAATCTTA_TTCGTGCA)


title: K562_H3k27me3_20181025_icell8_418_(20181025_Pool2_AAGAGGCA_CGTACCAA)


title: K562_H3k27me3_20181025_icell8_412_(20181025_Pool2_TGCTGGGT_TAGATCGC)


title: K562_H3k27me3_20181025_icell8_410_(20181025_Pool2_CAGAGAGG_ATTGGGTC)


title: K562_H3k27me3_20181025_icell8_411_(20181025_Pool2_AGTAGATT_AGGTTGCC)


title: K562_H3k27me3_20181025_icell8_415_(20181025_Pool2_CCTCGCAG_AGAGATCT)


title: K562_H3k27me3_20181025_icell8_431_(20181025_Pool2_CAGAGAGG_CGGACTGC)


title: K562_H3k27me3_20181025_icell8_407_(20181025_Pool2_AAATACAG_TTTCTAGC)


title: K562_H3k27me3_20181025_icell8_424_(20181025_Pool2_CCAAATAA_TCACATGG)


title: K562_H3k27me3_20181025_icell8_405_(20181025_Pool2_ATGGGAAC_ATGGTCAT)


title: K562_H3k27me3_20181025_icell8_403_(20181025_Pool2_GAGCACGT_GGGCTTGG)


title: K562_H3k27me3_20181025_icell8_406_(20181025_Pool2_AAATACAG_ATGGTCAT)


title: K562_H3k27me3_20181025_icell8_408_(20181025_Pool2_AACGCCAT_TCCGAGTT)


title: K562_H3k27me3_20181025_icell8_404_(20181025_Pool2_CCTCGCAG_CCACCGTT)


title: K562_H3k27me3_20181025_icell8_402_(20181025_Pool2_CATCGACC_ACATTCGT)


title: K562_H3k27me3_20181025_icell8_399_(20181025_Pool2_TTTCGTCA_TACTGACC)


title: K562_H3k27me3_20181025_icell8_398_(20181025_Pool2_CTCTCTAC_CAGCAACG)


title: K562_H3k27me3_20181025_icell8_397_(20181025_Pool2_AAGAGGCA_CGGACTGC)


title: K562_H3k27me3_20181025_icell8_395_(20181025_Pool2_AAGAGGCA_AAGGAGTA)


title: K562_H3k27me3_20181025_icell8_394_(20181025_Pool2_CATCGACC_CGGACTGC)


title: K562_H3k27me3_20181025_icell8_393_(20181025_Pool2_TGGTCACA_TTCGTGCA)


title: K562_H3k27me3_20181025_icell8_388_(20181025_Pool2_AAGAGGCA_ACTTGTTG)


title: K562_H3k27me3_20181025_icell8_392_(20181025_Pool2_GTGATAGC_ACTGCATA)


title: K562_H3k27me3_20181025_icell8_386_(20181025_Pool2_TCCTGAGC_TATTTGCG)


title: K562_H3k27me3_20181025_icell8_387_(20181025_Pool2_CATCGACC_GGTTCAAT)


title: K562_H3k27me3_20181025_icell8_389_(20181025_Pool2_CAGAGAGG_GACGTGGC)


title: K562_H3k27me3_20181025_icell8_390_(20181025_Pool2_AGGCAGAA_AGAGATCT)


title: K562_H3k27me3_20181025_icell8_385_(20181025_Pool2_AGGTTGGG_TAACCAAG)


title: K562_H3k27me3_20181025_icell8_391_(20181025_Pool2_CCTCGCAG_TATCCTCT)


title: K562_H3k27me3_20181025_icell8_383_(20181025_Pool2_TCCTGAGC_GACGTGGC)


title: K562_H3k27me3_20181025_icell8_382_(20181025_Pool2_CCAAATAA_CCCTTGGA)


title: K562_H3k27me3_20181025_icell8_381_(20181025_Pool2_CTCTCTAC_AGTCCGAG)


title: K562_H3k27me3_20181025_icell8_401_(20181025_Pool2_ATAAGTTA_CTAGAAGT)


title: K562_H3k27me3_20181025_icell8_400_(20181025_Pool2_TGCTGGGT_TCCGAGTT)


title: K562_H3k27me3_20181025_icell8_375_(20181025_Pool2_GTAGAGGA_TCACATGG)


title: K562_H3k27me3_20181025_icell8_379_(20181025_Pool2_GAGCACGT_ACTTGTTG)


title: K562_H3k27me3_20181025_icell8_380_(20181025_Pool2_CCTCGCAG_TGGAAATC)


title: K562_H3k27me3_20181025_icell8_377_(20181025_Pool2_AGGCAGAA_GAGACGTG)


title: K562_H3k27me3_20181025_icell8_378_(20181025_Pool2_CAATCTTA_ACTTGTTG)


title: K562_H3k27me3_20181025_icell8_374_(20181025_Pool2_CAGAGAGG_GTAAGGAG)


title: K562_H3k27me3_20181025_icell8_373_(20181025_Pool2_GACGCTCC_GTAAGGAG)


title: K562_H3k27me3_20181025_icell8_396_(20181025_Pool2_AGGTTGGG_TGATTGTT)


title: K562_H3k27me3_20181025_icell8_370_(20181025_Pool2_CCTCGCAG_ATTGGGTC)


title: K562_H3k27me3_20181025_icell8_369_(20181025_Pool2_AAGAGGCA_ATCCAGGA)


title: K562_H3k27me3_20181025_icell8_371_(20181025_Pool2_AGGCAGAA_TCAAGAGC)


title: K562_H3k27me3_20181025_icell8_368_(20181025_Pool2_GTGATAGC_ACTTGTTG)


title: K562_H3k27me3_20181025_icell8_384_(20181025_Pool2_TGTGCCTT_CCAGTTTA)


title: K562_H3k27me3_20181025_icell8_367_(20181025_Pool2_GTAGAGGA_AGAGATCT)


title: K562_H3k27me3_20181025_icell8_366_(20181025_Pool2_TCCTGAGC_AGTCCGAG)


title: K562_H3k27me3_20181025_icell8_365_(20181025_Pool2_GTAGAGGA_GAACTCGA)


title: K562_H3k27me3_20181025_icell8_364_(20181025_Pool2_AGGTTGGG_TGGGCGGT)


title: K562_H3k27me3_20181025_icell8_362_(20181025_Pool2_GTGATAGC_CGGACTGC)


title: K562_H3k27me3_20181025_icell8_361_(20181025_Pool2_TCAGCTAT_AAGGAGTA)


title: K562_H3k27me3_20181025_icell8_363_(20181025_Pool2_AGGTTGGG_CTAAGCCT)


title: K562_H3k27me3_20181025_icell8_376_(20181025_Pool2_TCCTGAGC_GCCCACGT)


title: K562_H3k27me3_20181025_icell8_360_(20181025_Pool2_CTCTCTAC_AACATGAT)


title: K562_H3k27me3_20181025_icell8_358_(20181025_Pool2_CAGAGAGG_ACCGGCCC)


title: K562_H3k27me3_20181025_icell8_372_(20181025_Pool2_AGGCAGAA_TAACCAAG)


title: K562_H3k27me3_20181025_icell8_356_(20181025_Pool2_AACGCCAT_ATGGTCAT)


title: K562_H3k27me3_20181025_icell8_354_(20181025_Pool2_GGCGTCGA_CCCTTGGA)


title: K562_H3k27me3_20181025_icell8_352_(20181025_Pool2_ATGATGAT_TGGAAATC)


title: K562_H3k27me3_20181025_icell8_357_(20181025_Pool2_ATGGGAAC_GAACTCGA)


title: K562_H3k27me3_20181025_icell8_353_(20181025_Pool2_CCAACACG_TATTTGCG)


title: K562_H3k27me3_20181025_icell8_350_(20181025_Pool2_GTAGAGGA_ACTTGTTG)


title: K562_H3k27me3_20181025_icell8_348_(20181025_Pool2_AAGAGGCA_AGGTTGCC)


title: K562_H3k27me3_20181025_icell8_345_(20181025_Pool2_CTCATGGG_AATCTACA)


title: K562_H3k27me3_20181025_icell8_344_(20181025_Pool2_CAGAGAGG_CCTGCGGG)


title: K562_H3k27me3_20181025_icell8_347_(20181025_Pool2_CAATCTTA_ATCGAATG)


title: K562_H3k27me3_20181025_icell8_349_(20181025_Pool2_TCCTGAGC_ATTGGGTC)


title: K562_H3k27me3_20181025_icell8_346_(20181025_Pool2_CATCGACC_CTAAGCCT)


title: K562_H3k27me3_20181025_icell8_343_(20181025_Pool2_GTGATAGC_GGCACGGA)


title: K562_H3k27me3_20181025_icell8_341_(20181025_Pool2_CTCATGGG_AGCCGTTC)


title: K562_H3k27me3_20181025_icell8_340_(20181025_Pool2_TGTGCCTT_AGGTTGCC)


title: K562_H3k27me3_20181025_icell8_355_(20181025_Pool2_AGGCAGAA_ACCGGCCC)


title: K562_H3k27me3_20181025_icell8_339_(20181025_Pool2_TTGACCCT_TACTGACC)


title: K562_H3k27me3_20181025_icell8_359_(20181025_Pool2_AGACCAGC_TTCGTGCA)


title: K562_H3k27me3_20181025_icell8_342_(20181025_Pool2_ATCACTCG_TACTGACC)


title: K562_H3k27me3_20181025_icell8_351_(20181025_Pool2_GTATTCGG_CGCCAGAT)


title: K562_H3k27me3_20181025_icell8_333_(20181025_Pool2_ATGGGAAC_GCATTAAG)


title: K562_H3k27me3_20181025_icell8_335_(20181025_Pool2_TCAGCTAT_GAGACGTG)


title: K562_H3k27me3_20181025_icell8_334_(20181025_Pool2_TGTGCCTT_ACGATAAC)


title: K562_H3k27me3_20181025_icell8_331_(20181025_Pool2_TGGTCACA_GTGTATCG)


title: K562_H3k27me3_20181025_icell8_330_(20181025_Pool2_CCAACACG_GTGTATCG)


title: K562_H3k27me3_20181025_icell8_329_(20181025_Pool2_CCTCGCAG_ACGATAAC)


title: K562_H3k27me3_20181025_icell8_332_(20181025_Pool2_CATCGACC_CAGCAACG)


title: K562_H3k27me3_20181025_icell8_326_(20181025_Pool2_AGGTTGGG_AGCCGTTC)


title: K562_H3k27me3_20181025_icell8_325_(20181025_Pool2_AGACCAGC_GGTTCAAT)


title: K562_H3k27me3_20181025_icell8_327_(20181025_Pool2_AGGTTGGG_AGGTTGCC)


title: K562_H3k27me3_20181025_icell8_338_(20181025_Pool2_AGGCAGAA_CCTTATGT)


title: K562_H3k27me3_20181025_icell8_324_(20181025_Pool2_AGTAGATT_TCACATGG)


title: K562_H3k27me3_20181025_icell8_322_(20181025_Pool2_ATGATGAT_CGGACTGC)


title: K562_H3k27me3_20181025_icell8_320_(20181025_Pool2_AATTCGTT_ATGGTCAT)


title: K562_H3k27me3_20181025_icell8_321_(20181025_Pool2_CCAACACG_CGGACTGC)


title: K562_H3k27me3_20181025_icell8_317_(20181025_Pool2_TGTGCCTT_GTAAGGAG)


title: K562_H3k27me3_20181025_icell8_323_(20181025_Pool2_CTCTCTAC_TGATGAAA)


title: K562_H3k27me3_20181025_icell8_316_(20181025_Pool2_GTGATAGC_GTGTATCG)


title: K562_H3k27me3_20181025_icell8_318_(20181025_Pool2_AACGCCAT_GGCACGGA)


title: K562_H3k27me3_20181025_icell8_336_(20181025_Pool2_TGGTCACA_ATGGTCAT)


title: K562_H3k27me3_20181025_icell8_337_(20181025_Pool2_CCTCGCAG_TAACCAAG)


title: K562_H3k27me3_20181025_icell8_328_(20181025_Pool2_CCAAATAA_CGCCAGAT)


title: K562_H3k27me3_20181025_icell8_310_(20181025_Pool2_AAGAGGCA_CCTTATGT)


title: K562_H3k27me3_20181025_icell8_313_(20181025_Pool2_ATGGGAAC_TGTTCCTG)


title: K562_H3k27me3_20181025_icell8_307_(20181025_Pool2_TTTCGTCA_ATGGTCAT)


title: K562_H3k27me3_20181025_icell8_309_(20181025_Pool2_GTGATAGC_TGTTCCTG)


title: K562_H3k27me3_20181025_icell8_319_(20181025_Pool2_TCAGCTAT_AGGTTGCC)


title: K562_H3k27me3_20181025_icell8_308_(20181025_Pool2_TTTCGTCA_ACCGGCCC)


title: K562_H3k27me3_20181025_icell8_314_(20181025_Pool2_CTCTCTAC_GTGTATCG)


title: K562_H3k27me3_20181025_icell8_305_(20181025_Pool2_GAGCACGT_AGCCGTTC)


title: K562_H3k27me3_20181025_icell8_306_(20181025_Pool2_AGTAGATT_ACTGCATA)


title: K562_H3k27me3_20181025_icell8_303_(20181025_Pool2_ATGGGAAC_CGTACCAA)


title: K562_H3k27me3_20181025_icell8_302_(20181025_Pool2_ATGGGAAC_CCACCGTT)


title: K562_H3k27me3_20181025_icell8_301_(20181025_Pool2_CCAACACG_AGAGTAGA)


title: K562_H3k27me3_20181025_icell8_315_(20181025_Pool2_CATCGACC_CCAGTTTA)


title: K562_H3k27me3_20181025_icell8_299_(20181025_Pool2_ATAAGTTA_CCTGCGGG)


title: K562_H3k27me3_20181025_icell8_298_(20181025_Pool2_GTAGAGGA_CCTGCGGG)


title: K562_H3k27me3_20181025_icell8_300_(20181025_Pool2_CTCATGGG_ACTTGTTG)


title: K562_H3k27me3_20181025_icell8_297_(20181025_Pool2_TCCTGAGC_TAGATCGC)


title: K562_H3k27me3_20181025_icell8_312_(20181025_Pool2_CAATCTTA_GGGCTTGG)


title: K562_H3k27me3_20181025_icell8_296_(20181025_Pool2_TCCTGAGC_GTGTATCG)


title: K562_H3k27me3_20181025_icell8_295_(20181025_Pool2_AGTAGATT_GAACTCGA)


title: K562_H3k27me3_20181025_icell8_292_(20181025_Pool2_CATCGACC_AGAGATCT)


title: K562_H3k27me3_20181025_icell8_304_(20181025_Pool2_GCGTTAAA_ATTACCGT)


title: K562_H3k27me3_20181025_icell8_293_(20181025_Pool2_ATGATGAT_CTCTCTAT)


title: K562_H3k27me3_20181025_icell8_290_(20181025_Pool2_ATCACTCG_CTTTAACA)


title: K562_H3k27me3_20181025_icell8_291_(20181025_Pool2_CTCTCTAC_AGAGATCT)


title: K562_H3k27me3_20181025_icell8_294_(20181025_Pool2_AGGTTGGG_AGAGTAGA)


title: K562_H3k27me3_20181025_icell8_289_(20181025_Pool2_AGGTTGGG_GGTGAAGG)


title: K562_H3k27me3_20181025_icell8_288_(20181025_Pool2_AGGCAGAA_GGTTCAAT)


title: K562_H3k27me3_20181025_icell8_286_(20181025_Pool2_TTTCGTCA_AGTCCGAG)


title: K562_H3k27me3_20181025_icell8_285_(20181025_Pool2_ATCACTCG_GCCCACGT)


title: K562_H3k27me3_20181025_icell8_287_(20181025_Pool2_TGGTCACA_GGTGAAGG)


title: K562_H3k27me3_20181025_icell8_283_(20181025_Pool2_CATCGACC_GCATTAAG)


title: K562_H3k27me3_20181025_icell8_284_(20181025_Pool2_GTGATAGC_TACTGACC)


title: K562_H3k27me3_20181025_icell8_280_(20181025_Pool2_CTCATGGG_AGTCCGAG)


title: K562_H3k27me3_20181025_icell8_279_(20181025_Pool2_GTAGAGGA_CTTTAACA)


title: K562_H3k27me3_20181025_icell8_282_(20181025_Pool2_CAATCTTA_GCATTAAG)


title: K562_H3k27me3_20181025_icell8_281_(20181025_Pool2_CTCTCTAC_TAAGTTAC)


title: K562_H3k27me3_20181025_icell8_277_(20181025_Pool2_CCAAATAA_ATGGTCAT)


title: K562_H3k27me3_20181025_icell8_278_(20181025_Pool2_TTGACCCT_GCAGCCTC)


title: K562_H3k27me3_20181025_icell8_276_(20181025_Pool2_ATCACTCG_CAGCAACG)


title: K562_H3k27me3_20181025_icell8_274_(20181025_Pool2_CCAAATAA_CCTGCGGG)


title: K562_H3k27me3_20181025_icell8_271_(20181025_Pool2_TGTGCCTT_CCACCGTT)


title: K562_H3k27me3_20181025_icell8_272_(20181025_Pool2_AGGTTGGG_GCAGCCTC)


title: K562_H3k27me3_20181025_icell8_270_(20181025_Pool2_GTAGAGGA_TATCCTCT)


title: K562_H3k27me3_20181025_icell8_269_(20181025_Pool2_CAGAGAGG_AGCCGTTC)


title: K562_H3k27me3_20181025_icell8_268_(20181025_Pool2_TGTGCCTT_TGCATACT)


title: K562_H3k27me3_20181025_icell8_267_(20181025_Pool2_CCAAATAA_TTAACTTG)


title: K562_H3k27me3_20181025_icell8_266_(20181025_Pool2_CTCTCTAC_AGAGTAGA)


title: K562_H3k27me3_20181025_icell8_265_(20181025_Pool2_TTTCGTCA_ATTGGGTC)


title: K562_H3k27me3_20181025_icell8_260_(20181025_Pool2_GAGCACGT_TCCGAGTT)


title: K562_H3k27me3_20181025_icell8_275_(20181025_Pool2_AGGTTGGG_TCCATCAA)


title: K562_H3k27me3_20181025_icell8_264_(20181025_Pool2_TTTCGTCA_ACGATAAC)


title: K562_H3k27me3_20181025_icell8_261_(20181025_Pool2_CAGAGAGG_AGGTTGCC)


title: K562_H3k27me3_20181025_icell8_258_(20181025_Pool2_AAATACAG_AAGGAGTA)


title: K562_H3k27me3_20181025_icell8_262_(20181025_Pool2_TCCTGAGC_AACATGAT)


title: K562_H3k27me3_20181025_icell8_259_(20181025_Pool2_ATCACTCG_ATCCAGGA)


title: K562_H3k27me3_20181025_icell8_257_(20181025_Pool2_TTGACCCT_AATCTACA)


title: K562_H3k27me3_20181025_icell8_256_(20181025_Pool2_GAGCACGT_TTAACTTG)


title: K562_H3k27me3_20181025_icell8_250_(20181025_Pool2_GACGCTCC_TGTTCCTG)


title: K562_H3k27me3_20181025_icell8_251_(20181025_Pool2_CCAAATAA_GCAGCCTC)


title: K562_H3k27me3_20181025_icell8_254_(20181025_Pool2_GACGCTCC_GATCATTC)


title: K562_H3k27me3_20181025_icell8_249_(20181025_Pool2_GGCGTCGA_GTCGGACT)


title: K562_H3k27me3_20181025_icell8_252_(20181025_Pool2_TCCTGAGC_ACTTAGAA)


title: K562_H3k27me3_20181025_icell8_247_(20181025_Pool2_CCAAATAA_TTTCTAGC)


title: K562_H3k27me3_20181025_icell8_246_(20181025_Pool2_AGGCAGAA_CGCCAGAT)


title: K562_H3k27me3_20181025_icell8_253_(20181025_Pool2_TCCTGAGC_TCCATCAA)


title: K562_H3k27me3_20181025_icell8_255_(20181025_Pool2_ATGATGAT_TTCGTGCA)


title: K562_H3k27me3_20181025_icell8_241_(20181025_Pool2_TGTGCCTT_ATGGTCAT)


title: K562_H3k27me3_20181025_icell8_239_(20181025_Pool2_CTCTCTAC_ACTGCATA)


title: K562_H3k27me3_20181025_icell8_238_(20181025_Pool2_CTCTCTAC_ATTGGGTC)


title: K562_H3k27me3_20181025_icell8_234_(20181025_Pool2_AAATACAG_GTAAGGAG)


title: K562_H3k27me3_20181025_icell8_232_(20181025_Pool2_GTGATAGC_TTTCTAGC)


title: K562_H3k27me3_20181025_icell8_237_(20181025_Pool2_GTGATAGC_ATGGTCAT)


title: K562_H3k27me3_20181025_icell8_236_(20181025_Pool2_AACGCCAT_GCAGCCTC)


title: K562_H3k27me3_20181025_icell8_233_(20181025_Pool2_CAATCTTA_GAACTCGA)


title: K562_H3k27me3_20181025_icell8_230_(20181025_Pool2_TCCTGAGC_CAGCAACG)


title: K562_H3k27me3_20181025_icell8_245_(20181025_Pool2_CTCTCTAC_TCCGAGTT)


title: K562_H3k27me3_20181025_icell8_244_(20181025_Pool2_AACGCCAT_AGAGTAGA)


title: K562_H3k27me3_20181025_icell8_228_(20181025_Pool2_CTCATGGG_ACGATAAC)


title: K562_H3k27me3_20181025_icell8_235_(20181025_Pool2_TGCTGGGT_TTAACTTG)


title: K562_H3k27me3_20181025_icell8_243_(20181025_Pool2_CTCTCTAC_GACGTGGC)


title: K562_H3k27me3_20181025_icell8_248_(20181025_Pool2_GACGCTCC_TAAGTTAC)


title: K562_H3k27me3_20181025_icell8_222_(20181025_Pool2_TTTAATGC_TTTCTAGC)


title: K562_H3k27me3_20181025_icell8_227_(20181025_Pool2_CTCTCTAC_TCAAGAGC)


title: K562_H3k27me3_20181025_icell8_223_(20181025_Pool2_ATGGGAAC_AGGTTGCC)


title: K562_H3k27me3_20181025_icell8_221_(20181025_Pool2_CAGAGAGG_CCCTTGGA)


title: K562_H3k27me3_20181025_icell8_224_(20181025_Pool2_AGGTTGGG_GATCATTC)


title: K562_H3k27me3_20181025_icell8_225_(20181025_Pool2_CTCATGGG_TCAAGAGC)


title: K562_H3k27me3_20181025_icell8_311_(20181025_Pool2_GAGCACGT_GATTCCCA)


title: K562_H3k27me3_20181025_icell8_219_(20181025_Pool2_AACGCCAT_CGGACTGC)


title: K562_H3k27me3_20181025_icell8_229_(20181025_Pool2_AACGCCAT_TAAGTTAC)


title: K562_H3k27me3_20181025_icell8_218_(20181025_Pool2_GGCGTCGA_TATTTGCG)


title: K562_H3k27me3_20181025_icell8_217_(20181025_Pool2_TGTGCCTT_GCAAATTT)


title: K562_H3k27me3_20181025_icell8_220_(20181025_Pool2_CAGAGAGG_CGTAGACC)


title: K562_H3k27me3_20181025_icell8_240_(20181025_Pool2_AAGAGGCA_GAGACGTG)


title: K562_H3k27me3_20181025_icell8_216_(20181025_Pool2_GGCGTCGA_AACATGAT)


title: K562_H3k27me3_20181025_icell8_242_(20181025_Pool2_AGACCAGC_ACTTGTTG)


title: K562_H3k27me3_20181025_icell8_214_(20181025_Pool2_CCAAATAA_AGAGATCT)


title: K562_H3k27me3_20181025_icell8_213_(20181025_Pool2_GAGCACGT_GGTGAAGG)


title: K562_H3k27me3_20181025_icell8_209_(20181025_Pool2_AGGCAGAA_GACGTGGC)


title: K562_H3k27me3_20181025_icell8_210_(20181025_Pool2_AGGCAGAA_GCAGCCTC)


title: K562_H3k27me3_20181025_icell8_211_(20181025_Pool2_TTCGCGGC_TGGAAATC)


title: K562_H3k27me3_20181025_icell8_226_(20181025_Pool2_AGTAGATT_CTAGAAGT)


title: K562_H3k27me3_20181025_icell8_206_(20181025_Pool2_CAGAGAGG_ACTTAGAA)


title: K562_H3k27me3_20181025_icell8_205_(20181025_Pool2_AAGAGGCA_GCAAATTT)


title: K562_H3k27me3_20181025_icell8_204_(20181025_Pool2_TGCTGGGT_GGTTCAAT)


title: K562_H3k27me3_20181025_icell8_203_(20181025_Pool2_CAGAGAGG_AAGGAGTA)


title: K562_H3k27me3_20181025_icell8_202_(20181025_Pool2_ATCACTCG_TTAACTTG)


title: K562_H3k27me3_20181025_icell8_201_(20181025_Pool2_GCGTTAAA_AAGGAGTA)


title: K562_H3k27me3_20181025_icell8_208_(20181025_Pool2_GTATTCGG_ACGATAAC)


title: K562_H3k27me3_20181025_icell8_198_(20181025_Pool2_TTCGCGGC_TGTTCCTG)


title: K562_H3k27me3_20181025_icell8_199_(20181025_Pool2_CCTCGCAG_ATTACCGT)


title: K562_H3k27me3_20181025_icell8_215_(20181025_Pool2_CATCGACC_CGCCAGAT)


title: K562_H3k27me3_20181025_icell8_196_(20181025_Pool2_GCGTTAAA_CTCTCTAT)


title: K562_H3k27me3_20181025_icell8_194_(20181025_Pool2_AGGTTGGG_TTTCTAGC)


title: K562_H3k27me3_20181025_icell8_197_(20181025_Pool2_ATGATGAT_GGTTCAAT)


title: K562_H3k27me3_20181025_icell8_195_(20181025_Pool2_AGTAGATT_TGCATACT)


title: K562_H3k27me3_20181025_icell8_200_(20181025_Pool2_TGTGCCTT_TCTGGCGA)


title: K562_H3k27me3_20181025_icell8_212_(20181025_Pool2_CCAAATAA_AGCCGTTC)


title: K562_H3k27me3_20181025_icell8_191_(20181025_Pool2_GAGCACGT_GCAGCCTC)


title: K562_H3k27me3_20181025_icell8_190_(20181025_Pool2_TTGACCCT_ACATTCGT)


title: K562_H3k27me3_20181025_icell8_192_(20181025_Pool2_AGGCAGAA_TTTCTAGC)


title: K562_H3k27me3_20181025_icell8_189_(20181025_Pool2_AGACCAGC_TGCATACT)


title: K562_H3k27me3_20181025_icell8_188_(20181025_Pool2_TTGACCCT_GGCACGGA)


title: K562_H3k27me3_20181025_icell8_207_(20181025_Pool2_GACGCTCC_TAACCAAG)


title: K562_H3k27me3_20181025_icell8_187_(20181025_Pool2_AGACCAGC_AGTCCGAG)


title: K562_H3k27me3_20181025_icell8_193_(20181025_Pool2_AGGTTGGG_CGTACCAA)


title: K562_H3k27me3_20181025_icell8_185_(20181025_Pool2_ATGATGAT_TAGATCGC)


title: K562_H3k27me3_20181025_icell8_186_(20181025_Pool2_AGTAGATT_GATCATTC)


title: K562_H3k27me3_20181025_icell8_184_(20181025_Pool2_GTATTCGG_CTCTCTAT)


title: K562_H3k27me3_20181025_icell8_181_(20181025_Pool2_AGGTTGGG_CTAGAAGT)


title: K562_H3k27me3_20181025_icell8_182_(20181025_Pool2_AATTCGTT_CTAGAAGT)


title: K562_H3k27me3_20181025_icell8_183_(20181025_Pool2_AGTAGATT_CTCTCTAT)


title: K562_H3k27me3_20181025_icell8_179_(20181025_Pool2_ATAAGTTA_TTTCTAGC)


title: K562_H3k27me3_20181025_icell8_180_(20181025_Pool2_AGACCAGC_ACGCGCAG)


title: K562_H3k27me3_20181025_icell8_178_(20181025_Pool2_TTCGCGGC_AAGGAGTA)


title: K562_H3k27me3_20181025_icell8_175_(20181025_Pool2_ATGATGAT_AGAGATCT)


title: K562_H3k27me3_20181025_icell8_174_(20181025_Pool2_AGTAGATT_CTAAGCCT)


title: K562_H3k27me3_20181025_icell8_176_(20181025_Pool2_AGGCAGAA_ACATTCGT)


title: K562_H3k27me3_20181025_icell8_173_(20181025_Pool2_CTCATGGG_GATCATTC)


title: K562_H3k27me3_20181025_icell8_170_(20181025_Pool2_AACGCCAT_TTTCTAGC)


title: K562_H3k27me3_20181025_icell8_172_(20181025_Pool2_CAGAGAGG_CCTTATGT)


title: K562_H3k27me3_20181025_icell8_167_(20181025_Pool2_GTAGAGGA_AACATGAT)


title: K562_H3k27me3_20181025_icell8_169_(20181025_Pool2_TGCTGGGT_GTAAGGAG)


title: K562_H3k27me3_20181025_icell8_165_(20181025_Pool2_AGGTTGGG_CCTTATGT)


title: K562_H3k27me3_20181025_icell8_273_(20181025_Pool2_TGGTCACA_GGCACGGA)


title: K562_H3k27me3_20181025_icell8_168_(20181025_Pool2_CCAAATAA_CAGCAACG)


title: K562_H3k27me3_20181025_icell8_163_(20181025_Pool2_GAGCACGT_CTTTAACA)


title: K562_H3k27me3_20181025_icell8_164_(20181025_Pool2_AACGCCAT_GGGCTTGG)


title: K562_H3k27me3_20181025_icell8_166_(20181025_Pool2_TCCTGAGC_GAACTCGA)


title: K562_H3k27me3_20181025_icell8_160_(20181025_Pool2_GTAGAGGA_GCAGCCTC)


title: K562_H3k27me3_20181025_icell8_159_(20181025_Pool2_TCAGCTAT_GTGTATCG)


title: K562_H3k27me3_20181025_icell8_157_(20181025_Pool2_TGCTGGGT_TTTCTAGC)


title: K562_H3k27me3_20181025_icell8_158_(20181025_Pool2_ATCACTCG_TTTCTAGC)


title: K562_H3k27me3_20181025_icell8_171_(20181025_Pool2_CTCTCTAC_GCAGCCTC)


title: K562_H3k27me3_20181025_icell8_161_(20181025_Pool2_CCTCGCAG_TTAACTTG)


title: K562_H3k27me3_20181025_icell8_162_(20181025_Pool2_ATCACTCG_ATTGGGTC)


title: K562_H3k27me3_20181025_icell8_154_(20181025_Pool2_AGGCAGAA_CTAAGCCT)


title: K562_H3k27me3_20181025_icell8_155_(20181025_Pool2_TGCTGGGT_GATCATTC)


title: K562_H3k27me3_20181025_icell8_177_(20181025_Pool2_CAATCTTA_CCTGCGGG)


title: K562_H3k27me3_20181025_icell8_156_(20181025_Pool2_GGCGTCGA_TGTTCCTG)


title: K562_H3k27me3_20181025_icell8_151_(20181025_Pool2_TTTAATGC_ACATTCGT)


title: K562_H3k27me3_20181025_icell8_153_(20181025_Pool2_GCGTTAAA_GCAGCCTC)


title: K562_H3k27me3_20181025_icell8_150_(20181025_Pool2_GTAGAGGA_TACTGACC)


title: K562_H3k27me3_20181025_icell8_148_(20181025_Pool2_TTGACCCT_GTGTATCG)


title: K562_H3k27me3_20181025_icell8_149_(20181025_Pool2_AAGAGGCA_CAGCAACG)


title: K562_H3k27me3_20181025_icell8_146_(20181025_Pool2_CAGAGAGG_ACGATAAC)


title: K562_H3k27me3_20181025_icell8_144_(20181025_Pool2_CATCGACC_CCTTATGT)


title: K562_H3k27me3_20181025_icell8_145_(20181025_Pool2_TGTGCCTT_TAACCAAG)


title: K562_H3k27me3_20181025_icell8_141_(20181025_Pool2_AAGAGGCA_CCCTTGGA)


title: K562_H3k27me3_20181025_icell8_135_(20181025_Pool2_GTATTCGG_CTTTAACA)


title: K562_H3k27me3_20181025_icell8_138_(20181025_Pool2_TCCTGAGC_CCTTATGT)


title: K562_H3k27me3_20181025_icell8_152_(20181025_Pool2_TCCTGAGC_TATCCTCT)


title: K562_H3k27me3_20181025_icell8_134_(20181025_Pool2_ATAAGTTA_GTAAGGAG)


title: K562_H3k27me3_20181025_icell8_139_(20181025_Pool2_TTCGCGGC_GAACTCGA)


title: K562_H3k27me3_20181025_icell8_142_(20181025_Pool2_GACGCTCC_GGTTCAAT)


title: K562_H3k27me3_20181025_icell8_137_(20181025_Pool2_TGCTGGGT_ACGATAAC)


title: K562_H3k27me3_20181025_icell8_131_(20181025_Pool2_TTTCGTCA_CTAAGCCT)


title: K562_H3k27me3_20181025_icell8_130_(20181025_Pool2_AGACCAGC_ACGATAAC)


title: K562_H3k27me3_20181025_icell8_132_(20181025_Pool2_ATCACTCG_CCTGCGGG)


title: K562_H3k27me3_20181025_icell8_129_(20181025_Pool2_TGTGCCTT_AATCTACA)


title: K562_H3k27me3_20181025_icell8_127_(20181025_Pool2_ATCACTCG_ACATTCGT)


title: K562_H3k27me3_20181025_icell8_124_(20181025_Pool2_AGTAGATT_CCTTATGT)


title: K562_H3k27me3_20181025_icell8_126_(20181025_Pool2_TGTGCCTT_ACTGCATA)


title: K562_H3k27me3_20181025_icell8_128_(20181025_Pool2_TTCGCGGC_TTAACTTG)


title: K562_H3k27me3_20181025_icell8_123_(20181025_Pool2_AATTCGTT_TAAGTTAC)


title: K562_H3k27me3_20181025_icell8_125_(20181025_Pool2_GTATTCGG_ACCGGCCC)


title: K562_H3k27me3_20181025_icell8_147_(20181025_Pool2_AGGCAGAA_TAAGTTAC)


title: K562_H3k27me3_20181025_icell8_143_(20181025_Pool2_TTTCGTCA_AAGGAGTA)


title: K562_H3k27me3_20181025_icell8_122_(20181025_Pool2_TTGACCCT_TCTGGCGA)


title: K562_H3k27me3_20181025_icell8_121_(20181025_Pool2_AGGCAGAA_CGTAGACC)


title: K562_H3k27me3_20181025_icell8_231_(20181025_Pool2_TGGTCACA_GGGCTTGG)


title: K562_H3k27me3_20181025_icell8_136_(20181025_Pool2_ATAAGTTA_TCCGAGTT)


title: K562_H3k27me3_20181025_icell8_133_(20181025_Pool2_AGACCAGC_TGGAAATC)


title: K562_H3k27me3_20181025_icell8_115_(20181025_Pool2_CAATCTTA_ATCCAGGA)


title: K562_H3k27me3_20181025_icell8_114_(20181025_Pool2_TGTGCCTT_GCATTAAG)


title: K562_H3k27me3_20181025_icell8_118_(20181025_Pool2_AGGCAGAA_ACGCGCAG)


title: K562_H3k27me3_20181025_icell8_117_(20181025_Pool2_AGTAGATT_ATGGTCAT)


title: K562_H3k27me3_20181025_icell8_112_(20181025_Pool2_GTAGAGGA_GATCATTC)


title: K562_H3k27me3_20181025_icell8_110_(20181025_Pool2_AGGTTGGG_TATTTGCG)


title: K562_H3k27me3_20181025_icell8_116_(20181025_Pool2_GGCGTCGA_TTGCCTAA)


title: K562_H3k27me3_20181025_icell8_119_(20181025_Pool2_CCAAATAA_CTCTCTAT)


title: K562_H3k27me3_20181025_icell8_113_(20181025_Pool2_AAGAGGCA_GGCACGGA)


title: K562_H3k27me3_20181025_icell8_108_(20181025_Pool2_TCCTGAGC_TTGCCTAA)


title: K562_H3k27me3_20181025_icell8_109_(20181025_Pool2_AACGCCAT_TAACCAAG)


title: K562_H3k27me3_20181025_icell8_106_(20181025_Pool2_CAGAGAGG_TACTGACC)


title: K562_H3k27me3_20181025_icell8_263_(20181025_Pool2_TCAGCTAT_CGCCAGAT)


title: K562_H3k27me3_20181025_icell8_111_(20181025_Pool2_AGACCAGC_CGGACTGC)


title: K562_H3k27me3_20181025_icell8_103_(20181025_Pool2_TCCTGAGC_CTAGAAGT)


title: K562_H3k27me3_20181025_icell8_99_(20181025_Pool2_AGGTTGGG_ATGGTCAT)


title: K562_H3k27me3_20181025_icell8_105_(20181025_Pool2_TTGACCCT_AGTCCGAG)


title: K562_H3k27me3_20181025_icell8_120_(20181025_Pool2_CCAACACG_TTAACTTG)


title: K562_H3k27me3_20181025_icell8_102_(20181025_Pool2_GGCGTCGA_TGGAAATC)


title: K562_H3k27me3_20181025_icell8_101_(20181025_Pool2_GGCGTCGA_AGAGATCT)


title: K562_H3k27me3_20181025_icell8_100_(20181025_Pool2_TTTAATGC_CCAGTTTA)


title: K562_H3k27me3_20181025_icell8_104_(20181025_Pool2_ATAAGTTA_ACTGCATA)


title: K562_H3k27me3_20181025_icell8_98_(20181025_Pool2_AATTCGTT_TCCGAGTT)


title: K562_H3k27me3_20181025_icell8_97_(20181025_Pool2_AATTCGTT_GGCACGGA)


title: K562_H3k27me3_20181025_icell8_95_(20181025_Pool2_CCAAATAA_ACTGCATA)


title: K562_H3k27me3_20181025_icell8_96_(20181025_Pool2_GAGCACGT_GGTTCAAT)


title: K562_H3k27me3_20181025_icell8_93_(20181025_Pool2_TCCTGAGC_GATTCCCA)


title: K562_H3k27me3_20181025_icell8_107_(20181025_Pool2_ATCACTCG_AACATGAT)


title: K562_H3k27me3_20181025_icell8_88_(20181025_Pool2_TTGACCCT_AGAGATCT)


title: K562_H3k27me3_20181025_icell8_94_(20181025_Pool2_CAGAGAGG_TTAACTTG)


title: K562_H3k27me3_20181025_icell8_90_(20181025_Pool2_GGCGTCGA_AAGGAGTA)


title: K562_H3k27me3_20181025_icell8_92_(20181025_Pool2_CAATCTTA_CCCTTGGA)


title: K562_H3k27me3_20181025_icell8_89_(20181025_Pool2_GTAGAGGA_TGCATACT)


title: K562_H3k27me3_20181025_icell8_91_(20181025_Pool2_GTAGAGGA_TAGATCGC)


title: K562_H3k27me3_20181025_icell8_83_(20181025_Pool2_CTCATGGG_AGAGATCT)


title: K562_H3k27me3_20181025_icell8_86_(20181025_Pool2_ATCACTCG_GCATTAAG)


title: K562_H3k27me3_20181025_icell8_80_(20181025_Pool2_TGTGCCTT_TCAAGAGC)


title: K562_H3k27me3_20181025_icell8_87_(20181025_Pool2_TCCTGAGC_TTAACTTG)


title: K562_H3k27me3_20181025_icell8_79_(20181025_Pool2_AATTCGTT_CTCTCTAT)


title: K562_H3k27me3_20181025_icell8_82_(20181025_Pool2_AACGCCAT_TCACATGG)


title: K562_H3k27me3_20181025_icell8_84_(20181025_Pool2_TTTAATGC_AGGTTGCC)


title: K562_H3k27me3_20181025_icell8_78_(20181025_Pool2_CATCGACC_AACATGAT)


title: K562_H3k27me3_20181025_icell8_76_(20181025_Pool2_CAATCTTA_CGCCAGAT)


title: K562_H3k27me3_20181025_icell8_77_(20181025_Pool2_GCGTTAAA_TATCCTCT)


title: K562_H3k27me3_20181025_icell8_75_(20181025_Pool2_TTGACCCT_TAACCAAG)


title: K562_H3k27me3_20181025_icell8_74_(20181025_Pool2_CAATCTTA_AGAGATCT)


title: K562_H3k27me3_20181025_icell8_69_(20181025_Pool2_GGCGTCGA_ACGATAAC)


title: K562_H3k27me3_20181025_icell8_85_(20181025_Pool2_GTATTCGG_GCAAATTT)


title: K562_H3k27me3_20181025_icell8_67_(20181025_Pool2_GAGCACGT_CGCCAGAT)


title: K562_H3k27me3_20181025_icell8_70_(20181025_Pool2_GACGCTCC_ACTGCATA)


title: K562_H3k27me3_20181025_icell8_68_(20181025_Pool2_TCCTGAGC_AGAGATCT)


title: K562_H3k27me3_20181025_icell8_66_(20181025_Pool2_GTAGAGGA_CCCTTGGA)


title: K562_H3k27me3_20181025_icell8_62_(20181025_Pool2_GCGTTAAA_GACGTGGC)


title: K562_H3k27me3_20181025_icell8_64_(20181025_Pool2_TTTAATGC_ACTGCATA)


title: K562_H3k27me3_20181025_icell8_63_(20181025_Pool2_AATTCGTT_TTGCCTAA)


title: K562_H3k27me3_20181025_icell8_60_(20181025_Pool2_AGACCAGC_GGCACGGA)


title: K562_H3k27me3_20181025_icell8_61_(20181025_Pool2_CAATCTTA_TGCATACT)


title: K562_H3k27me3_20181025_icell8_55_(20181025_Pool2_CCAAATAA_GTAAGGAG)


title: K562_H3k27me3_20181025_icell8_59_(20181025_Pool2_TGTGCCTT_AAGGAGTA)


title: K562_H3k27me3_20181025_icell8_56_(20181025_Pool2_TGCTGGGT_TGCATACT)


title: K562_H3k27me3_20181025_icell8_73_(20181025_Pool2_AAGAGGCA_TAGATCGC)


title: K562_H3k27me3_20181025_icell8_52_(20181025_Pool2_CAGAGAGG_CGTACCAA)


title: K562_H3k27me3_20181025_icell8_72_(20181025_Pool2_TCAGCTAT_CGTAGACC)


title: K562_H3k27me3_20181025_icell8_51_(20181025_Pool2_TTGACCCT_ACGCGCAG)


title: K562_H3k27me3_20181025_icell8_54_(20181025_Pool2_CATCGACC_TTGCCTAA)


title: K562_H3k27me3_20181025_icell8_71_(20181025_Pool2_AGACCAGC_TATTTGCG)


title: K562_H3k27me3_20181025_icell8_53_(20181025_Pool2_GTAGAGGA_AAGGAGTA)


title: K562_H3k27me3_20181025_icell8_49_(20181025_Pool2_TGTGCCTT_GCAGCCTC)


title: K562_H3k27me3_20181025_icell8_58_(20181025_Pool2_TTTAATGC_TAACCAAG)


title: K562_H3k27me3_20181025_icell8_50_(20181025_Pool2_GTGATAGC_TTGCCTAA)


title: K562_H3k27me3_20181025_icell8_46_(20181025_Pool2_ATAAGTTA_TCACATGG)


title: K562_H3k27me3_20181025_icell8_47_(20181025_Pool2_CTCATGGG_CGCCAGAT)


title: K562_H3k27me3_20181025_icell8_48_(20181025_Pool2_ATAAGTTA_TGGAAATC)


title: K562_H3k27me3_20181025_icell8_45_(20181025_Pool2_AGGTTGGG_ACCGGCCC)


title: K562_H3k27me3_20181025_icell8_81_(20181025_Pool2_TCCTGAGC_AAGGAGTA)


title: K562_H3k27me3_20181025_icell8_44_(20181025_Pool2_TGGTCACA_CGTAGACC)


title: K562_H3k27me3_20181025_icell8_43_(20181025_Pool2_CTCATGGG_CTTTAACA)


title: K562_H3k27me3_20181025_icell8_42_(20181025_Pool2_AGGTTGGG_CCACCGTT)


title: K562_H3k27me3_20181025_icell8_41_(20181025_Pool2_TGGTCACA_TTAACTTG)


title: K562_H3k27me3_20181025_icell8_57_(20181025_Pool2_TGGTCACA_CAGCAACG)


title: K562_H3k27me3_20181025_icell8_40_(20181025_Pool2_CAATCTTA_GATCATTC)


title: K562_H3k27me3_20181025_icell8_37_(20181025_Pool2_CTCTCTAC_CGTAGACC)


title: K562_H3k27me3_20181025_icell8_38_(20181025_Pool2_AGGCAGAA_GGCACGGA)


title: K562_H3k27me3_20181025_icell8_35_(20181025_Pool2_TTCGCGGC_GCAAATTT)


title: K562_H3k27me3_20181025_icell8_39_(20181025_Pool2_AATTCGTT_TTCGTGCA)


title: K562_H3k27me3_20181025_icell8_34_(20181025_Pool2_ATAAGTTA_GATCATTC)


title: K562_H3k27me3_20181025_icell8_140_(20181025_Pool2_TCAGCTAT_TAGATCGC)


title: K562_H3k27me3_20181025_icell8_30_(20181025_Pool2_GACGCTCC_TGATGAAA)


title: K562_H3k27me3_20181025_icell8_31_(20181025_Pool2_ATAAGTTA_CGATAGGG)


title: K562_H3k27me3_20181025_icell8_33_(20181025_Pool2_GACGCTCC_AGGTTGCC)


title: K562_H3k27me3_20181025_icell8_27_(20181025_Pool2_TTGACCCT_CCTGCGGG)


title: K562_H3k27me3_20181025_icell8_28_(20181025_Pool2_GGCGTCGA_TAGATCGC)


title: K562_H3k27me3_20181025_icell8_25_(20181025_Pool2_TGCTGGGT_CTAAGCCT)


title: K562_H3k27me3_20181025_icell8_32_(20181025_Pool2_AATTCGTT_ACGATAAC)


title: K562_H3k27me3_20181025_icell8_24_(20181025_Pool2_TTTAATGC_TTGCCTAA)


title: K562_H3k27me3_20181025_icell8_23_(20181025_Pool2_AACGCCAT_GAACTCGA)


title: K562_H3k27me3_20181025_icell8_21_(20181025_Pool2_CAATCTTA_GGCACGGA)


title: K562_H3k27me3_20181025_icell8_20_(20181025_Pool2_AAATACAG_CTTTAACA)


title: K562_H3k27me3_20181025_icell8_22_(20181025_Pool2_TTTAATGC_CCTTATGT)


title: K562_H3k27me3_20181025_icell8_19_(20181025_Pool2_ATGATGAT_GCAAATTT)


title: K562_H3k27me3_20181025_icell8_65_(20181025_Pool2_TCCTGAGC_TAACCAAG)


title: K562_H3k27me3_20181025_icell8_18_(20181025_Pool2_AGGCAGAA_TATCCTCT)


title: K562_H3k27me3_20181025_icell8_17_(20181025_Pool2_CAATCTTA_TAAGTTAC)


title: K562_H3k27me3_20181025_icell8_14_(20181025_Pool2_AATTCGTT_ACCGGCCC)


title: K562_H3k27me3_20181025_icell8_15_(20181025_Pool2_TTTCGTCA_GAACTCGA)


title: K562_H3k27me3_20181025_icell8_36_(20181025_Pool2_CATCGACC_ACTTAGAA)


title: K562_H3k27me3_20181025_icell8_13_(20181025_Pool2_GACGCTCC_CTTTAACA)


title: K562_H3k27me3_20181025_icell8_16_(20181025_Pool2_ATCACTCG_GCAAATTT)


title: K562_H3k27me3_20181025_icell8_26_(20181025_Pool2_TTCGCGGC_TGCATACT)


title: K562_H3k27me3_20181025_icell8_29_(20181025_Pool2_AACGCCAT_TTAACTTG)


title: ATAC_FLS_T24_rep2

city: New York

first: Christopher

last: Loh

state: NY

address: 515 E 71st ST

department: Research

institute: Hospital for Special Surgery

description: ATAC-seq

data_processing:
FastQC was used to evaluate quality of sequenced reads (http://www.bioinformatics.babraham.ac.uk/project/fastqc). Contaminated adaptor portions of sequenced reads were trimmed.
Sequenced reads were mapped to reference human genome (hg19 assembly) using Bowtie2 or CASAVA with default parameters.
Genome_build: hg19

source_name: Rheumatoid Arthritis (RA) patient derived Fibroblast-like Synoviocytes

growth_protocol: Fiborbloast-like synoviocytes were obtained from RA patients who underwent total knee replacement or elbow synovectomy (protocol approved by the Institutional Review Board of the Hospital for Special Surgery). Synovial tissue fragments were incubated with Liberase and fibroblast-like synoviocytes (FLS) cells were allowed to adhere to tissue culture dishes and passaged every 3-5 days (4-5 passages).

extract_protocol:
Nuclei were pelleted by centrifugation for 5 min at 500g, 4°C.
Assay for transposase-accessible chromatin using sequencing (ATAC-seq) protocol developed by (Buenrostro et al., 2013); single end protocol.

treatment_protocol: Cells were treated with or without TNF (20ng/ml) and I-BET(10ng/ml) at various time points (1h, 3h, 6h, 24h, 48h, 72h).

tag: treatment

value: TNF (24hr)

tag: tissue

value: RA Synovial Fluid

value: Fibroblast


title: ATAC_FLS_T3_rep2

description: ATAC-seq

value: TNF (3hr)


title: ATAC_FLS_T72_rep1

description: ATAC-seq

value: TNF (72hr)


title: K562_H3k27me3_20181025_icell8_6_(20181025_Pool2_CCAAATAA_CTAAGCCT)


title: K562_H3k27me3_20181025_icell8_8_(20181025_Pool2_AGGTTGGG_ACTTGTTG)


title: K562_H3k27me3_20181025_icell8_9_(20181025_Pool2_TTGACCCT_TTGCCTAA)


title: ATAC_FLS_Ctrl_rep2

description: ATAC-seq


title: K562_H3k27me3_20181025_icell8_11_(20181025_Pool2_CCAAATAA_GCATTAAG)


title: K562_H3k27me3_20181025_icell8_10_(20181025_Pool2_AAATACAG_ATTACCGT)


title: K562_H3k27me3_20181025_icell8_7_(20181025_Pool2_GGCGTCGA_TTAACTTG)


title: K562_H3k27me3_20181025_icell8_3_(20181025_Pool2_GGCGTCGA_GAGACGTG)


title: K562_H3k27me3_20181025_icell8_1_(20181025_Pool2_TTTCGTCA_TTGCCTAA)


title: K562_H3k27me3_20181025_icell8_5_(20181025_Pool2_ATAAGTTA_CCCTTGGA)


title: K562_H3k27me3_20181025_icell8_4_(20181025_Pool2_ATGATGAT_TTAACTTG)


title: K562_H3k27me3_20181025_icell8_12_(20181025_Pool2_CAATCTTA_TGATGAAA)


title: K562_H3k27me3_20181025_icell8_2_(20181025_Pool2_AGTAGATT_TTAACTTG)


title: ATAC_FLS_Ctrl_rep1

description: ATAC-seq


title: RNA_MF_T24

description: mRNA-seq

library_source: transcriptomic

data_processing:
FastQC was used to evaluate quality of sequenced reads (http://www.bioinformatics.babraham.ac.uk/project/fastqc). Contaminated adaptor portions of sequenced reads were trimmed(http://www.bioinformatics.babraham.ac.uk/project/trim_galore).
Sequenced reads were mapped to reference human genome (hg19 assembly) using STAR aligner (2.5.2b, Dobin et al., 2013 Bioinformatics) with default parameters.
DEseq2 was used to estimate the abundance of transcripts.
Genome_build: hg19

molecule: total RNA

source_name: Primary human monocyte-derived macrophages

growth_protocol: Peripheral blood mononuclear cells were obtained from the blood of healthy donors by density gradient centrifugation using Ficoll (Invitrogen) and a protocol approved by the Hospital for Special Surgery Institutional Review Board. CD14+ human monocytes were purified from PBMCs by positive selection using anti-CD14 magnetic beads as recommended by the manufacturer (Miltenyi Biotec). Monocytes were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Invitrogen) supplemented with 10% heat-inactivated defined FBS (HyClone), penicillin/streptomycin (Invitrogen), L-glutamine (Invitrogen), and 10 ng/mL human macrophage colony-stimulating factor (M-CSF; Peprotech). Fiborbloast-like synoviocytes were obtained from RA patients who underwent total knee replacement or elbow synovectomy (protocol approved by the Institutional Review Board of the Hospital for Special Surgery). Synovial tissue fragments were incubated with Liberase and fibroblast-like synoviocytes (FLS) cells were allowed to adhere to tissue culture dishes and passaged every 3-5 days (4-5 passages).

extract_protocol:
RNA extraction kit from QIAGEN, according to company's protocol
RNA libraries were prepared for sequencing using standard Illumina protocols

value: peripheral blood

value: monocytes


title: RNA_MF_T3

description: mRNA-seq


title: ChIP_FLS_H3K27ac_T72_rep2

data_processing:
FastQC was used to evaluate quality of sequenced reads (http://www.bioinformatics.babraham.ac.uk/project/fastqc). Contaminated adaptor portions of sequenced reads were trimmed.
Sequenced reads were mapped to reference human genome (hg19 assembly) using BWA with default parameters.
Genome_build: hg19

extract_protocol:
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody.
Libraries were prepared according to Illumina's instructions accompanying the TruSeq ChIP Library Preparation Kit.

value: anti-H3k27ac (Abcam ab4279)


title: RNA_MF_T6

description: mRNA-seq

value: TNF (6hr)


title: ChIP_FLS_H3K27ac_T3_rep2


title: ChIP_FLS_H3K27ac_T24_rep2


title: ChIP_FLS_H3K27ac_Ctrl_rep2


title: ChIP_FLS_H3K27ac_T72_rep1


title: ChIP_FLS_H3K27ac_Ctrl_rep1


title: ATAC_FLS_T72_rep2

description: ATAC-seq


title: RNA_MF_T1

description: mRNA-seq

value: TNF (1hr)


title: RNA_MF_Ctrl

description: mRNA-seq


title: K562_H3K27me3_(20190104_SH_Hs_M30_20181224)

description: CUT&Tag for H3K27me3 in K562 cells

source_name: CUT&Tag

extract_protocol:
Bench-top CUT&Tag: Cells were harvested, counted and centrifuged for 3 min at 600 x g at room temperature. Aliquots of up to 500,000 cells per final sample, were washed twice in 1.5 ml Wash Buffer (20 mM HEPES pH 7.5; 150 mM NaCl; 0.5 mM Spermidine; 1X Protease inhibitor cocktail) by gentle pipetting. Concanavalin A coated magnetic beads (Bangs Laboratories) were prepared as described [Skene, P.J. & Henikoff, S. Elife 6:e21856 (2017)] and 10 µl of activated beads were added per sample and incubated at RT for ~10 minutes. The unbound supernatant was removed and bead-bound cells were resuspended in 50-100 µl Dig-wash Buffer (20 mM HEPES pH 7.5; 150 mM NaCl; 0.5 mM Spermidine; 1X Protease inhibitor cocktail; 0.05% Digitonin) containing 2mM EDTA and a 1:50 dilution of the appropriate primary antibody. Primary antibody incubation was performed on a nutating platform for 2 hr at room temperature (RT) or up to several days at 4 oC. The primary antibody was removed by placing the tube on the magnet stand to clear and pulling off all of the liquid. To increase the number of Protein A binding sites for each bound antibody, an appropriate secondary antibody (such as Guinea Pig anti-Rabbit IgG antibody for a rabbit primary antibody) was diluted 1:50 in 50-100 µl of Dig-Wash buffer and cells were incubated at RT for 30 min. Cells were washed using the magnet stand 2-3x for 5 min in 1 ml Dig-Wash buffer to remove unbound antibodies. A 1:200 dilution of pA-Tn5 adapter complex (~0.04 µM) was prepared in Dig-med Buffer (0.05% Digitonin, 20 mM HEPES, pH 7.5, 300 mM NaCl, 0.5 mM Spermidine, 1X Protease inhibitor cocktail). After removing the liquid on the magnet stand, 50-100 µL was added to the cells with gentle vortexing, which was incubated with pA-Tn5 at RT for 1 hr. Cells were washed 2-3x in 1 ml Dig-med Buffer to remove unbound pA-Tn5 protein. Next, cells were resuspended in 90 µL Dig-med Buffer and 10 µL 100 mM MgCl2 was added with gentle vortexing, and incubated at 37 for 1h. To stop tagmentation, 45 µL 0.5M EDTA, 5.5 µL 10% SDS and 1 µL 20 mg/ml Proteinase K was added, and tubes were incubated at 55 oC for 30 min and then at 70 oC for 20 min to inactivate Proteinase K. A volume of 122 µL Ampure XP beads was added, vortexed, incubated at RT with beads for 15 min at RT, washed twice gently in 80% ethanol, and eluted in 30 µL 10 mM Tris pH 8.0.
To make libraries, 21 µL DNA was mixed with 2 µL of a universal i5 and a uniquely barcoded i7 primer [Buenostro, J.D. et al. Single-cell chromatin accessibility reveals principles of regulatory variation. Nature 523, 486-U264 (2015)], using a different barcode for each sample. A volume of 25 µL NEBNext HiFi 2x PCR Master mix was added and mixed. The sample was placed in Thermocycler with heated lid using the following cycling conditions: 72 °C for 5 min (gap filling); 98 °C for 30 sec; 14 cycles of 98 °C for 10 sec and 63 °C for 30 sec; final extension at 72°C for 1 min and hold at 8 oC. Post-PCR clean-up was performed by adding 1.1X volume of Ampure XP beads (Beckman Counter), and libraries were incubated with beads for 15 min at RT, washed twice gently in 80% ethanol, and eluted in 30 µL 10 mM Tris pH 8.0.


title: K562_H4Kac_(20190104_SH_Hs_H41_20181227)

description: CUT&Tag for H4Kac in K562 cells

value: H4Kac Millipore 06-598 Lot 29867


title: K562_NPAT_(20180919_HK_Hs_K5_NPAT_0912)

description: CUT&Tag for NPAT in 100000 K562 cells, replicates 3 and 4

extract_protocol:
High-throughput CUT&Tag: Cells were washed twice with Wash Buffer (20 mM HEPES pH 7.5; 150 mM NaCl; 0.5 mM Spermidine; 1X Protease inhibitor cocktail) and resuspended in Dig-wash (20 mM HEPES pH 7.5; 150 mM NaCl; 0.5 mM Spermidine; 1X Protease inhibitor cocktail; 0.05% Digitonin) containing 2mM EDTA and arrayed in a 96-well plate, 25 µL per well. Permeabilization before antibody incubation varied from 1 to 5 hours. Then, 25 µL 1:25 dilutions of appropriate antibodies in Dig-wash-EDTA were added. Cells were incubated with primary antibodies overnight at 4 oC. Concanavalin A (ConA) coated magnetic beads (Bangs Laboratories) were prepared as described [Skene, P.J. & Henikoff, S. Elife 6:e21856 (2017)]. Ten µL of activated ConA-coated magnetic beads were added to each sample, mixed gently and incubated at room temperature for 10 minutes. The plate was placed on a magnetic plate holder and supernatants were discarded. An appropriate secondary antibody (Guinea Pig anti-Rabbit IgG Heavy & Light Chain antibody, Antibodies-Online ABIN101961) was prepared as a 1:50 dilution in Dig-wash and added to each well. Cells were washed 3 times with Dig-wash and then incubated with a 1:200 dilution of pA-Tn5 adapter complex (Kaya-Okur et al., CUT&Tag for efficient epigenomic profiling of small samples and single cells, manuscript submitted) in Dig-med (0.05% Digitonin, 20 mM HEPES, pH 7.5, 300 mM NaCl, 0.5 mM Spermidine, 1X Protease inhibitor cocktail) at RT for 1 hr. Cells were washed 3x for 5 min in Dig-med, resuspended in 50 µL Tagmentation buffer (Dig-med + 10 mM MgCl2) and incubated at 37 for 1h. To stop tagmentation, 2.25 µL 0.5M EDTA, 2.75 µL 10% SDS and 0.5 µL 20 mg/ml Proteinase K was added to the sample, which was incubated at 55 oC for 30 min and then at 70 oC for 20 min to inactivate the Proteinase K. Samples were held at 4oC overnight until ready to continue. A 1.1x volume of AMPure XP beads was added to each well, vortexed and incubated at room temperature for 10-15 min. The plate was placed on magnet and unbound liquid was removed. Beads were gently rinsed twice with 80% ethanol, and DNA was eluted with 35 µL of 10 mM Tris-HCl pH 8. A 21 µL sample was mixed with 2 μL of a universal i5 and a uniquely barcoded i7 primer [Buenostro, J.D. et al. Nature 523, 486-U264 (2015)], using a different barcode for each sample. A volume of 25 μL NEBNext HiFi 2x PCR Master mix was added and mixed. The sample was placed in a Thermocycler with heated lid using the following cycling conditions: 72 °C for 5 min (gap filling); 98 °C for 30 sec; 14 cycles of 98 °C for 10 sec and 63 °C for 30 sec; final extension at 72°C for 1 min and hold at 8 oC. Post-PCR clean-up was performed by adding a 1.1X volume of Ampure XP beads (Beckman Counter), and libraries were incubated with the beads for 15 min at RT, washed twice gently in 80% ethanol, and eluted in 30 μL 10 mM Tris pH 8.0. All 96 individually i7-barcoded libraries were mixed in equal proportions for sequencing on an Illumina HiSeq 2500 2-lane (Turbo) flow-cell.
High-throughput CUT&Tag

value: NPAT Thermo Fisher cat# PA5-66839


title: K562_IgG_(20180919_HK_Hs_K5_IgG_0912)

description: CUT&Tag for IgG in 100000 K562 cells

value: IgG Rbt anti Ms H&L (Abcam, ab46540)


title: K562_H3K9ac_(20190104_SH_Hs_H91,2_181227)

description: CUT&Tag for H3K9ac in K562 cells

value: H3K9ac Millipore 06-942 Lot 21897


title: K562_H2AZ_(20180919_HK_Hs_K5_H2AZ_0912)

description: CUT&Tag for H2AZ in 100000 K562 cells

value: H2A.Z Active Motif 39113

value: human immortalised myelogenous leukemia cell line


title: K562_CTCF_500mM_(20190104_SH_Hs_C50_20181224,6)

description: CUT&Tag for CTCF in K562 cells using 500 mM NaCl

value: CTCF Millipore 07-729 Lot 2971000


title: K562_CTCF_300mM_(20190104_SH_Hs_C30_20181224,6)

description: CUT&Tag for CTCF in K562 cells using 300 mM NaCl


title: K562_CTCF_150mM_(20190104_SH_Hs_C15_20181224,6)

description: CUT&Tag for CTCF in K562 cells using 150 mM NaCl


title: H1_IgG_(20180919_HK_Hs_H1_IgG_0912)

description: CUT&Tag for IgG in 100000 H1 ES cells

value: human embryonic stem cells


title: K562_H3K4me1_Abcam_8895_(20180810_SH_Hs_H3K4me1_0806)

description: CUT&Tag for H3K4me1

value: H3K4me1 Abcam 8895

value: K562 human immortalised myelogenous leukemia cell line


title: K562_H3K27me3_CST9733_fresh_500th_(20190227_SH_Hs_me3A4_20190217)

description: CUT&Tag for H3K27me3 using 1:500 pA-Tn5

value: H3K27me3 Cell Signaling Technologies #9733


title: K562_CTCF_(20180919_HK_Hs_K5_CTCF_0912)

description: CUT&Tag for CTCF in 100000 K562 cells, replicates 1 and 2

extract_protocol:
High-throughput CUT&Tag: Cells were washed twice with Wash Buffer (20 mM HEPES pH 7.5; 150 mM NaCl; 0.5 mM Spermidine; 1X Protease inhibitor cocktail) and resuspended in Dig-wash (20 mM HEPES pH 7.5; 150 mM NaCl; 0.5 mM Spermidine; 1X Protease inhibitor cocktail; 0.05% Digitonin) containing 2mM EDTA and arrayed in a 96-well plate, 25 µL per well. Permeabilization before antibody incubation varied from 1 to 5 hours. Then, 25 µL 1:25 dilutions of appropriate antibodies in Dig-wash-EDTA were added. Cells were incubated with primary antibodies overnight at 4 oC. Concanavalin A (ConA) coated magnetic beads (Bangs Laboratories) were prepared as described [Skene, P.J. & Henikoff, S. Elife 6:e21856 (2017)]. Ten µL of activated ConA-coated magnetic beads were added to each sample, mixed gently and incubated at room temperature for 10 minutes. The plate was placed on a magnetic plate holder and supernatants were discarded. An appropriate secondary antibody (Guinea Pig anti-Rabbit IgG Heavy & Light Chain antibody, Antibodies-Online ABIN101961) was prepared as a 1:50 dilution in Dig-wash and added to each well. Cells were washed 3 times with Dig-wash and then incubated with a 1:200 dilution of pA-Tn5 adapter complex (Kaya-Okur et al.,  CUT&Tag for efficient epigenomic profiling of small samples and single cells, manuscript submitted) in Dig-med (0.05% Digitonin, 20 mM HEPES, pH 7.5, 300 mM NaCl, 0.5 mM Spermidine, 1X Protease inhibitor cocktail) at RT for 1 hr. Cells were washed 3x for 5 min in Dig-med, resuspended in 50 µL Tagmentation buffer (Dig-med + 10 mM MgCl2) and incubated at 37 for 1h. To stop tagmentation, 2.25 µL 0.5M EDTA, 2.75 µL 10% SDS and 0.5 µL 20 mg/ml Proteinase K was added to the sample, which was incubated at 55 oC for 30 min and then at 70 oC for 20 min to inactivate the Proteinase K. Samples were held at 4oC overnight until ready to continue. A 1.1x volume of AMPure XP beads was added to each well, vortexed and incubated at room temperature for 10-15 min. The plate was placed on magnet and unbound liquid was removed. Beads were gently rinsed twice with 80% ethanol, and DNA was eluted with 35 µL of 10 mM Tris-HCl pH 8. A 21 µL sample was mixed with 2 μL of a universal i5 and a uniquely barcoded i7 primer [Buenostro, J.D. et al. Nature 523, 486-U264 (2015)], using a different barcode for each sample. A volume of 25 μL NEBNext HiFi 2x PCR Master mix was added and mixed. The sample was placed in a Thermocycler with heated lid using the following cycling conditions: 72 °C for 5 min (gap filling); 98 °C for 30 sec; 14 cycles of 98 °C for 10 sec and 63 °C for 30 sec; final extension at 72°C for 1 min and hold at 8 oC. Post-PCR clean-up was performed by adding a 1.1X volume of Ampure XP beads (Beckman Counter), and libraries were incubated with the beads for 15 min at RT, washed twice gently in 80% ethanol, and eluted in 30 μL 10 mM Tris pH 8.0. All 96 individually i7-barcoded libraries were mixed in equal proportions for sequencing on an Illumina HiSeq 2500 2-lane (Turbo) flow-cell.
High-throughput CUT&Tag

value: CTCF Millipore 07-729


title: K562_NPAT_Thermo-PA5-66839_fresh_250th_(20190227_SH_Hs_NPA2_20190217)

description: CUT&Tag for NPAT using 1:250 pA-Tn5

value: NPAT Thermo-Fisher PA5-66839


title: K562_NPAT_Thermo-PA5-66839_fresh_125th_(20190227_SH_Hs_NPA1_20190217)

description: CUT&Tag for NPAT using 1:125 pA-Tn5


title: K562_H3K27me3_CST9733_fresh_1000th_(20190227_SH_Hs_me3A8_20190217)

description: CUT&Tag for H3K27me3 using 1:1000 pA-Tn5


title: K562_NPAT_Thermo-PA5-66839_10d-RT_500th_(20190227_SH_Hs_NPB4_20190217)

description: CUT&Tag for NPAT using 1:500 10-day-RT pA-Tn5


title: K562_H3K27me3_CST9733_10d-RT_500th_(20190227_SH_Hs_me3B4_20190217)

description: CUT&Tag for H3K27me3 using 1:500 10-day-RT pA-Tn5


title: K562_H3K27me3_CST9733_fresh_250th_(20190227_SH_Hs_me3A2_20190217)

description: CUT&Tag for H3K27me3 using 1:250 pA-Tn5


title: K562_NPAT_Thermo-PA5-66839_10d-RT_1000th_(20190227_SH_Hs_NPB8_20190217)

description: CUT&Tag for NPAT using 1:1000 10-day-RT pA-Tn5


title: K562_NPAT_Thermo-PA5-66839_10d-RT_250th_(20190227_SH_Hs_NPB2_20190217)

description: CUT&Tag for NPAT using 1:250 10-day-RT pA-Tn5


title: K562_H3K4me1_ActMot_39113_(20180817_SH_Hs_7_H2AZ_0816)

description: CUT&Tag for H2A.Z


title: K562_H3K4me2_Upstate_07-030_(20180817_SH_Hs_11_K4me2_0816)

description: CUT&Tag for H3K4me2

value: H3K4me2 Upstate 07-030


title: K562_IgG_ABIN101961_(20190104_SH_Hs_IgG_20181224)

description: CUT&Tag for IgG in 100000 K562 cells

value: Gp anti-Rb IgG Antibodies Online cat# ABIN101961


title: K562_H3K27me3_CST9733_10d-RT_250th_(20190227_SH_Hs_me3B2_20190217)

description: CUT&Tag for H3K27me3 using 1:250 10-day-RT pA-Tn5


title: K562_H3K27me3_CST9733_10d-RT_1000th_(20190227_SH_Hs_me3B8_20190217)

description: CUT&Tag for H3K27me3 using 1:1000 10-day-RT pA-Tn5


title: K562_H3K27me3_CST9733_10d-RT_125th_(20190227_SH_Hs_me3B1_20190217)

description: CUT&Tag for H3K27me3 using 1:125 10-day-RT pA-Tn5


title: K562_NPAT_Thermo-PA5-66839_10d-RT_125th_(20190227_SH_Hs_NPB1_20190217)

description: CUT&Tag for NPAT using 1:125 10-day-RT pA-Tn5


title: K562_NPAT_Thermo-PA5-66839_fresh_1000th_(20190227_SH_Hs_NPA8_20190217)

description: CUT&Tag for NPAT using 1:1000 pA-Tn5


title: K562_H3K4me3_Abcam_ab8580_(20180817_SH_Hs_12_K4me3_0816)

description: CUT&Tag for H3K4me3

value: H3K4me3 Abcam 8580


title: K562_H3K27me3_CST9733_fresh_125th_(20190227_SH_Hs_me3A1_20190217)

description: CUT&Tag for H3K27me3 using 1:125 pA-Tn5


title: K562_NPAT_Thermo-PA5-66839_fresh_500th_(20190227_SH_Hs_NPA4_20190217)

description: CUT&Tag for NPAT using 1:500 pA-Tn5


title: K562_H3K4me2_Rep2_20181202_icell8_390_(20181206_new_CGTACTAGAATCTACA)

description: Single-cell CUT&Tag for H3K4me2 in K562 cells using ICELL8 platform

extract_protocol: Single-cell CUT&Tag: Approximately 50,000 exponentially growing K562 cells were processed by centrifugation between buffer and reagent exchanges in low-retention tubes throughout. Centrifugations were performed at 600g for 3 min in a swinging bucket rotor for the initial wash and incubation steps, and then at 300g for 3 min after pA-Tn5 binding.  Cells were collected and washed twice with 1ml Wash Buffer (20 mM HEPES, pH 7.5; 150 mM NaCl; 0.5 mM Spermidine, 1X Protease inhibitor cocktail) at room temperature. Nuclei were isolated by permeabilizing cells in NP40-Digitonin Wash Buffer (0.01% NP40, 0.01% Digitonin in wash buffer) and resuspended in 1 mL of NP40-Digitonin Wash buffer with 1mM EDTA. Antibody was added at a 1:50 dilution and incubated on a rotator at 4°C overnight. Permeabilized cells were then rinsed once with NP40-Digitonin Wash buffer and incubated with anti-Rabbit IgG antibody (1:50 dilution) in 1 ml of NP40-Digitonin Wash buffer on a rotator at room temperature for 30 min. Nuclei were then washed 3x for 5 min in 1 ml NP40-Digitonin Wash buffer to remove unbound antibodies. For pA-Tn5 binding, a 1:100 dilution of pA-Tn5 adapter complex was prepared in 1 mL NP40-Dig-med-buffer (0.01% NP40, 0.01% Digitonin, 20 mM HEPES, pH 7.5, 300 mM NaCl, 0.5 mM Spermidine, 1X Protease inhibitor cocktail), and permeabilized cells were incubated with the pA-Tn5 adapter complex on a rotator at RT for 1 hr. Cells were washed 3x for 5 min in 1 ml NP40-Dig-med-buffer to remove excess pA-Tn5 protein. Cells were resuspended in 150 µL Tagmentation buffer (10 mM MgCl2 in NP40-Dig-med-buffer) and incubated at 37 for 1h. Tagmentation was stopped by adding 50 µL of 4X Stop Buffer (40.4 mM EDTA and 2 mg/ml DAPI) and the sample was held on ice for 30 minutes. The SMARTer ICELL8 single-cell system (Takara Bio USA, Cat. #640000) was used to array single cells as described for scATAC-seq12. DAPI-stained nuclei were visualized under the microscope and if there were clumps, they were strained through 10 micron cell strainers. Cells were counted using a hematocytometer and diluted at 25 cells/µL in 0.5X PBS and 1X Second Diluent (Takara Bio USA, Cat. # 640196). Cells were loaded to a source loading plate. Control wells containing 0.5X PBS (25 µl) and fiducial mix (25 µl) (Takara Bio USA, Cat.  #640196) were also included in the source loading plate. Using the ICELL8 MultiSample NanoDispenser (MSND) FLA program, cells were dispensed into a SMARTer ICELL8 350v chip (Takara Bio USA, Cat. # 640019) at 35 nanoliter per well. After cell dispense was complete, chips were sealed with the imaging film (Takara Bio USA, Cat. #640109) and centrifuged at 400 xg for 5 min at room temperature and imaged using the ICELL8 imaging station (Takara Bio USA). Images were analyzed using automated microscopy image analysis software (CellSelect, Takara Bio USA). Since cells were stained only with DAPI, they were propidium iodide negative, so that permeabilized cells would not be excluded by default software settings. Additional single cells were manually selected for dispensing using a manual triaging procedure. Immediately following imaging, the filter file, which notes single-cell containing wells and control wells, was generated. We typically obtained ~1000 single cells per chip. All of the following reagents were added to the selected set of wells which contained single cells. To index the whole chip, 72 i5 and 72 i7 unique indices [Buenostro, J.D. et al. Single-cell chromatin accessibility reveals principles of regulatory variation. Nature 523, 486-U264 (2015)] were dispensed at 7.32 µM using ICELL8 MSND FLA program using the index 1 and index 2 filtered dispense tool respectively at 35 nanoliter per well. NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541L) was dispensed twice using the ICELL8 MSND Single Cell / TCR program for the filtered dispense tool at 50 nanoliter per well. The chip was sealed and centrifuged at 2250 xg at 4◦C for at least 5 min after each dispense. The chip was sealed with a TE Sealing film (Takara Bio USA, Cat. #640109) and on-chip PCR was performed using a SMARTer ICELL8 Thermal Cycler (Takara Bio USA) as follows: 5 min at 72 ◦C and 2 min at 98 ◦C followed by 15 cycles of 10 sec at 98 ◦C, 30 sec at 60 ◦C, and 5 s at 72 ◦C, with a final extension at 72 ◦C for 1 min. PCR products were collected by centrifugation at ~2250 xg for 20 min using the supplied SMARTer ICELL8 Collection Kit (Takara Bio USA, Cat.#640048). Pooled libraries were purified using Ampure XP beads (Beckman Counter) in a 1:1.1 ratio. Briefly, libraries were incubated with beads for 15 min at RT, washed twice in 80% ethanol, and eluted in 10 mM Tris pH 8.0. Paired-end 25x8x8x25 bp Illumina sequencing was performed on the pooled barcoded libraries following the manufacturer’s instructions.

value: H3K4me2 Upstate cat# 07-030


title: K562_H3K4me2_Rep2_20181202_icell8_391_(20181206_new_TACACCTCTCTGGCGA)


title: K562_H3K4me2_Rep2_20181202_icell8_389_(20181206_new_CATCCTGTATTGGGTC)


title: K562_H3K4me2_Rep2_20181202_icell8_392_(20181206_new_TAGGCATGAGAGATCT)


title: K562_H3K4me2_Rep2_20181202_icell8_393_(20181206_new_CGAGGCTGAGCCGTTC)


title: K562_H3K4me2_Rep2_20181202_icell8_388_(20181206_new_GAAATGCCCCAGTTTA)


title: K562_H3K4me2_Rep2_20181202_icell8_387_(20181206_new_ATTGCCGGTACTGACC)


title: K562_H3K4me2_Rep2_20181202_icell8_386_(20181206_new_TAGGCATGACGCGCAG)


title: K562_H3K4me2_Rep2_20181202_icell8_374_(20181206_new_TCTTATTAATCGAATG)


title: K562_H3K4me2_Rep2_20181202_icell8_366_(20181206_new_GACTTAGTCGCCAGAT)


title: K562_H3K4me2_Rep2_20181202_icell8_375_(20181206_new_CGAGGCTGCTAAGCCT)


title: K562_H3K4me2_Rep2_20181202_icell8_383_(20181206_new_GGACTCCTCTTTAACA)


title: K562_H3K4me2_Rep2_20181202_icell8_370_(20181206_new_GGTCATTTGAACTCGA)


title: K562_H3K4me2_Rep2_20181202_icell8_376_(20181206_new_CGTACTAGATTGGGTC)


title: K562_H3K4me2_Rep2_20181202_icell8_371_(20181206_new_CGAGGCTGATTGGGTC)


title: K562_H3K4me2_Rep2_20181202_icell8_378_(20181206_new_GAAATGCCGGTGAAGG)


title: K562_H3K4me2_Rep2_20181202_icell8_382_(20181206_new_TGGGTTTCATCGAATG)


title: K562_H3K4me2_Rep2_20181202_icell8_373_(20181206_new_GGTAAACCATCGAATG)


title: K562_H3K4me2_Rep2_20181202_icell8_384_(20181206_new_AATGGTAGAGAGATCT)


title: K562_H3K4me2_Rep2_20181202_icell8_372_(20181206_new_ACAAAGTGTCCGAGTT)


title: K562_H3K4me2_Rep2_20181202_icell8_369_(20181206_new_CTTGGGTCATTGGGTC)


title: K562_H3K4me2_Rep2_20181202_icell8_364_(20181206_new_GCTACGCTTCCGAGTT)


title: K562_H3K4me2_Rep2_20181202_icell8_363_(20181206_new_CGGCCAATCTAAGCCT)


title: K562_H3K4me2_Rep2_20181202_icell8_381_(20181206_new_TAGGCATGTACTGACC)


title: K562_H3K4me2_Rep2_20181202_icell8_379_(20181206_new_CGAGGCTGTTGCCTAA)


title: K562_H3K4me2_Rep2_20181202_icell8_359_(20181206_new_GGACTCCTCTAGAAGT)


title: K562_H3K4me2_Rep2_20181202_icell8_377_(20181206_new_TACTTGAAGTCGGACT)


title: K562_H3K4me2_Rep2_20181202_icell8_365_(20181206_new_GACTTAGTCTAAGCCT)


title: K562_H3K4me2_Rep2_20181202_icell8_360_(20181206_new_TTCCATATTGGAAATC)


title: K562_H3K4me2_Rep2_20181202_icell8_361_(20181206_new_GGTCATTTTAACCAAG)


title: K562_H3K4me2_Rep2_20181202_icell8_358_(20181206_new_CAAGAATTATCGAATG)


title: K562_H3K4me2_Rep2_20181202_icell8_380_(20181206_new_AATGGTAGTGGGCGGT)


title: K562_H3K4me2_Rep2_20181202_icell8_385_(20181206_new_TACTTGAAAGTCCGAG)


title: K562_H3K4me2_Rep2_20181202_icell8_368_(20181206_new_TTATGAAACGTACCAA)


title: K562_H3K4me2_Rep2_20181202_icell8_357_(20181206_new_TGGATCTGAGCCGTTC)


title: K562_H3K4me2_Rep2_20181202_icell8_362_(20181206_new_TAGGCATGTTAACTTG)


title: K562_H3K4me2_Rep2_20181202_icell8_367_(20181206_new_GCTACGCTGCCCACGT)


title: K562_H3K4me2_Rep2_20181202_icell8_356_(20181206_new_TGGATCTGGCCCACGT)


title: K562_H3K4me2_Rep2_20181202_icell8_355_(20181206_new_GTGTGGTGTCCATCAA)


title: K562_H3K4me2_Rep2_20181202_icell8_354_(20181206_new_AATGGTAGGGGCTTGG)


title: K562_H3K4me2_Rep2_20181202_icell8_350_(20181206_new_GACTTAGTAGAGATCT)


title: K562_H3K4me2_Rep2_20181202_icell8_343_(20181206_new_CGCGGACAATCGAATG)


title: K562_H3K4me2_Rep2_20181202_icell8_347_(20181206_new_TAGGCATGACATTCGT)


title: K562_H3K4me2_Rep2_20181202_icell8_348_(20181206_new_TACTTGAAACCGGCCC)


title: K562_H3K4me2_Rep2_20181202_icell8_352_(20181206_new_CCGTTTGTTCAAGAGC)


title: K562_H3K4me2_Rep2_20181202_icell8_351_(20181206_new_GACTTAGTTGGGCGGT)


title: K562_H3K4me2_Rep2_20181202_icell8_344_(20181206_new_GTGTGGTGCGGACTGC)


title: K562_H3K4me2_Rep2_20181202_icell8_353_(20181206_new_TAACAACACCTGCGGG)


title: K562_H3K4me2_Rep2_20181202_icell8_340_(20181206_new_GAAATGCCTAAGTTAC)


title: K562_H3K4me2_Rep2_20181202_icell8_346_(20181206_new_ACAAAGTGGATTCCCA)


title: K562_H3K4me2_Rep2_20181202_icell8_341_(20181206_new_TACACCTCATCGAATG)


title: K562_H3K4me2_Rep2_20181202_icell8_345_(20181206_new_GGAGTAAGGGCACGGA)


title: K562_H3K4me2_Rep2_20181202_icell8_342_(20181206_new_GACTTAGTTCTGGCGA)


title: K562_H3K4me2_Rep2_20181202_icell8_329_(20181206_new_TAACAACAGCCCACGT)


title: K562_H3K4me2_Rep2_20181202_icell8_334_(20181206_new_TGGGTTTCTCCGAGTT)


title: K562_H3K4me2_Rep2_20181202_icell8_333_(20181206_new_CGTACTAGACGCGCAG)


title: K562_H3K4me2_Rep2_20181202_icell8_330_(20181206_new_CGTACTAGCGATAGGG)


title: K562_H3K4me2_Rep2_20181202_icell8_339_(20181206_new_TGGGTTTCAGCCGTTC)


title: K562_H3K4me2_Rep2_20181202_icell8_328_(20181206_new_TGGATCTGATCGAATG)


title: K562_H3K4me2_Rep2_20181202_icell8_335_(20181206_new_TTGCTAAGAGAGTAGA)


title: K562_H3K4me2_Rep2_20181202_icell8_338_(20181206_new_CGGTTCCCGGGCTTGG)


title: K562_H3K4me2_Rep2_20181202_icell8_336_(20181206_new_GCTACGCTATCCAGGA)


title: K562_H3K4me2_Rep2_20181202_icell8_332_(20181206_new_GTTAACAGGTCGGACT)


title: K562_H3K4me2_Rep2_20181202_icell8_327_(20181206_new_TTGCTAAGCCAGTTTA)


title: K562_H3K4me2_Rep2_20181202_icell8_337_(20181206_new_TTGCTAAGGACGTGGC)


title: K562_H3K4me2_Rep2_20181202_icell8_349_(20181206_new_AATGGTAGTGGAAATC)


title: K562_H3K4me2_Rep2_20181202_icell8_331_(20181206_new_CCGTTTGTACGCGCAG)


title: K562_H3K4me2_Rep2_20181202_icell8_322_(20181206_new_GACTTAGTAGAGTAGA)


title: K562_H3K4me2_Rep2_20181202_icell8_324_(20181206_new_TACTTGAACCACCGTT)


title: K562_H3K4me2_Rep2_20181202_icell8_325_(20181206_new_TAAGGCGACGCCAGAT)


title: K562_H3K4me2_Rep2_20181202_icell8_323_(20181206_new_TAACAACATATCCTCT)


title: K562_H3K4me2_Rep2_20181202_icell8_319_(20181206_new_CCGTTTGTTCTGGCGA)


title: K562_H3K4me2_Rep2_20181202_icell8_321_(20181206_new_TCTTATTAGCAAATTT)


title: K562_H3K4me2_Rep2_20181202_icell8_317_(20181206_new_TGGATCTGACGATAAC)


title: K562_H3K4me2_Rep2_20181202_icell8_318_(20181206_new_TAAGGCGATGATGAAA)


title: K562_H3K4me2_Rep2_20181202_icell8_316_(20181206_new_GGACTCCTACGCGCAG)


title: K562_H3K4me2_Rep2_20181202_icell8_326_(20181206_new_GGACTCCTAGAGTAGA)


title: K562_H3K4me2_Rep2_20181202_icell8_320_(20181206_new_GGTAAACCTCACATGG)


title: K562_H3K4me2_Rep2_20181202_icell8_306_(20181206_new_TACACCTCTCAAGAGC)


title: K562_H3K4me2_Rep2_20181202_icell8_307_(20181206_new_AATGGTAGTAAGTTAC)


title: K562_H3K4me2_Rep2_20181202_icell8_305_(20181206_new_GGACTCCTCTAAGCCT)


title: K562_H3K4me2_Rep2_20181202_icell8_304_(20181206_new_CGAGGCTGTGGAAATC)


title: K562_H3K4me2_Rep2_20181202_icell8_301_(20181206_new_TCTTATTATCCATCAA)


title: K562_H3K4me2_Rep2_20181202_icell8_313_(20181206_new_GGTAAACCATTACCGT)


title: K562_H3K4me2_Rep2_20181202_icell8_314_(20181206_new_TACTTGAAATCCAGGA)


title: K562_H3K4me2_Rep2_20181202_icell8_312_(20181206_new_CGCGGACAACGATAAC)


title: K562_H3K4me2_Rep2_20181202_icell8_300_(20181206_new_CCGTTTGTCGATAGGG)


title: K562_H3K4me2_Rep2_20181202_icell8_311_(20181206_new_ACAAAGTGATTACCGT)


title: K562_H3K4me2_Rep2_20181202_icell8_302_(20181206_new_CCGTTTGTCTCTCTAT)


title: K562_H3K4me2_Rep2_20181202_icell8_309_(20181206_new_AAACGGTCACTTGTTG)


title: K562_H3K4me2_Rep2_20181202_icell8_299_(20181206_new_CGAGGCTGTAACCAAG)


title: K562_H3K4me2_Rep2_20181202_icell8_308_(20181206_new_GGTCATTTTCAAGAGC)


title: K562_H3K4me2_Rep2_20181202_icell8_297_(20181206_new_GAAATGCCTCTGGCGA)


title: K562_H3K4me2_Rep2_20181202_icell8_298_(20181206_new_GACTTAGTCCCTTGGA)


title: K562_H3K4me2_Rep2_20181202_icell8_315_(20181206_new_CTTGGGTCTACTGACC)


title: K562_H3K4me2_Rep2_20181202_icell8_294_(20181206_new_CGAGGCTGTATTTGCG)


title: K562_H3K4me2_Rep2_20181202_icell8_310_(20181206_new_GGTCATTTGTCGGACT)


title: K562_H3K4me2_Rep2_20181202_icell8_295_(20181206_new_GGTAAACCTATTTGCG)


title: K562_H3K4me2_Rep2_20181202_icell8_296_(20181206_new_TGGGTTTCACGCGCAG)


title: K562_H3K4me2_Rep2_20181202_icell8_290_(20181206_new_AATGGTAGGAGACGTG)


title: K562_H3K4me2_Rep2_20181202_icell8_287_(20181206_new_CGTACTAGCTAAGCCT)


title: K562_H3K4me2_Rep2_20181202_icell8_291_(20181206_new_TAACAACATAACCAAG)


title: K562_H3K4me2_Rep2_20181202_icell8_303_(20181206_new_TGGGTTTCCCTTATGT)


title: K562_H3K4me2_Rep2_20181202_icell8_289_(20181206_new_CGAGGCTGCAGCAACG)


title: K562_H3K4me2_Rep2_20181202_icell8_288_(20181206_new_AATGGTAGACTTAGAA)


title: K562_H3K4me2_Rep2_20181202_icell8_292_(20181206_new_GAAATGCCGACGTGGC)


title: K562_H3K4me2_Rep2_20181202_icell8_286_(20181206_new_TGGGTTTCCCACCGTT)


title: K562_H3K4me2_Rep2_20181202_icell8_285_(20181206_new_GCTACGCTTAAGTTAC)


title: K562_H3K4me2_Rep2_20181202_icell8_284_(20181206_new_CGGTTCCCCGTACCAA)


title: K562_H3K4me2_Rep2_20181202_icell8_283_(20181206_new_GTTAACAGGACGTGGC)


title: K562_H3K4me2_Rep2_20181202_icell8_281_(20181206_new_GGACTCCTATCCAGGA)


title: K562_H3K4me2_Rep2_20181202_icell8_276_(20181206_new_GAAATGCCGGGCTTGG)


title: K562_H3K4me2_Rep2_20181202_icell8_279_(20181206_new_CGAGGCTGTGGGCGGT)


title: K562_H3K4me2_Rep2_20181202_icell8_270_(20181206_new_CGGTTCCCATGGTCAT)


title: K562_H3K4me2_Rep2_20181202_icell8_271_(20181206_new_GATCCAAAGGCACGGA)


title: K562_H3K4me2_Rep2_20181202_icell8_272_(20181206_new_CATCCTGTTGATGAAA)


title: K562_H3K4me2_Rep2_20181202_icell8_275_(20181206_new_GGACTCCTTCCGAGTT)


title: K562_H3K4me2_Rep2_20181202_icell8_277_(20181206_new_GAAATGCCACTTAGAA)


title: K562_H3K4me2_Rep2_20181202_icell8_293_(20181206_new_AATGGTAGGACGTGGC)


title: K562_H3K4me2_Rep2_20181202_icell8_273_(20181206_new_CGAGGCTGTCTGGCGA)


title: K562_H3K4me2_Rep2_20181202_icell8_278_(20181206_new_GACTTAGTGGTTCAAT)


title: K562_H3K4me2_Rep2_20181202_icell8_264_(20181206_new_TAAGGCGATATCCTCT)


title: K562_H3K4me2_Rep2_20181202_icell8_265_(20181206_new_TTGCTAAGTAACCAAG)


title: K562_H3K4me2_Rep2_20181202_icell8_269_(20181206_new_GGACTCCTACTTGTTG)


title: K562_H3K4me2_Rep2_20181202_icell8_268_(20181206_new_TCTTATTATTAACTTG)


title: K562_H3K4me2_Rep2_20181202_icell8_267_(20181206_new_TTATGAAATATTTGCG)


title: K562_H3K4me2_Rep2_20181202_icell8_266_(20181206_new_TAGGCATGCTAAGCCT)


title: K562_H3K4me2_Rep2_20181202_icell8_280_(20181206_new_TTATGAAACAGCAACG)


title: K562_H3K4me2_Rep2_20181202_icell8_263_(20181206_new_CATCCTGTACGATAAC)


title: K562_H3K4me2_Rep2_20181202_icell8_262_(20181206_new_GAAATGCCTTCGTGCA)


title: K562_H3K4me2_Rep2_20181202_icell8_274_(20181206_new_GGTAAACCCGATAGGG)


title: K562_H3K4me2_Rep2_20181202_icell8_261_(20181206_new_TTCCATATGGCACGGA)


title: K562_H3K4me2_Rep2_20181202_icell8_259_(20181206_new_ACAAAGTGAGAGATCT)


title: K562_H3K4me2_Rep2_20181202_icell8_258_(20181206_new_CGGTTCCCAATCTACA)


title: K562_H3K4me2_Rep2_20181202_icell8_260_(20181206_new_GCTACGCTTAACCAAG)


title: K562_H3K4me2_Rep2_20181202_icell8_256_(20181206_new_CGTACTAGGAGACGTG)


title: K562_H3K4me2_Rep2_20181202_icell8_257_(20181206_new_TTATGAAAGGTGAAGG)


title: K562_H3K4me2_Rep2_20181202_icell8_255_(20181206_new_TACACCTCTACTGACC)


title: K562_H3K4me2_Rep2_20181202_icell8_253_(20181206_new_TGGGTTTCGATCATTC)


title: K562_H3K4me2_Rep2_20181202_icell8_282_(20181206_new_AATGGTAGCTCTCTAT)


title: K562_H3K4me2_Rep2_20181202_icell8_254_(20181206_new_TAACAACATGTTCCTG)


title: K562_H3K4me2_Rep2_20181202_icell8_252_(20181206_new_CGCGGACACTCTCTAT)


title: K562_H3K4me2_Rep2_20181202_icell8_247_(20181206_new_CGAGGCTGGTGTATCG)


title: K562_H3K4me2_Rep2_20181202_icell8_249_(20181206_new_GGGAATGACCTGCGGG)


title: K562_H3K4me2_Rep2_20181202_icell8_248_(20181206_new_GAAATGCCAGGTTGCC)


title: K562_H3K4me2_Rep2_20181202_icell8_250_(20181206_new_TTCCATATGCATTAAG)


title: K562_H3K4me2_Rep2_20181202_icell8_243_(20181206_new_GATCCAAATAAGTTAC)


title: K562_H3K4me2_Rep2_20181202_icell8_244_(20181206_new_TAGGCATGACTGCATA)


title: K562_H3K4me2_Rep2_20181202_icell8_245_(20181206_new_TTCCATATGAGACGTG)


title: K562_H3K4me2_Rep2_20181202_icell8_242_(20181206_new_GACTTAGTGCCCACGT)


title: K562_H3K4me2_Rep2_20181202_icell8_241_(20181206_new_TAAGGCGAGCAGCCTC)


title: K562_H3K4me2_Rep2_20181202_icell8_240_(20181206_new_ATTGCCGGTCCGAGTT)


title: K562_H3K4me2_Rep2_20181202_icell8_234_(20181206_new_CGTACTAGCCAGTTTA)


title: K562_H3K4me2_Rep2_20181202_icell8_235_(20181206_new_GACTTAGTAGGTTGCC)


title: K562_H3K4me2_Rep2_20181202_icell8_233_(20181206_new_GACTTAGTTAGATCGC)


title: K562_H3K4me2_Rep2_20181202_icell8_238_(20181206_new_TGGGTTTCTGGGCGGT)


title: K562_H3K4me2_Rep2_20181202_icell8_232_(20181206_new_CCGTTTGTGGGCTTGG)


title: K562_H3K4me2_Rep2_20181202_icell8_231_(20181206_new_GTTAACAGCCAGTTTA)


title: K562_H3K4me2_Rep2_20181202_icell8_230_(20181206_new_GGGAATGACGTAGACC)


title: K562_H3K4me2_Rep2_20181202_icell8_229_(20181206_new_AATGGTAGAGAGTAGA)


title: K562_H3K4me2_Rep2_20181202_icell8_236_(20181206_new_CGAGGCTGATTACCGT)


title: K562_H3K4me2_Rep2_20181202_icell8_239_(20181206_new_CGTACTAGGCATTAAG)


title: K562_H3K4me2_Rep2_20181202_icell8_228_(20181206_new_GCTACGCTGGGCTTGG)


title: K562_H3K4me2_Rep2_20181202_icell8_251_(20181206_new_TACTTGAAAGCCGTTC)


title: K562_H3K4me2_Rep2_20181202_icell8_226_(20181206_new_CCGTTTGTATTACCGT)


title: K562_H3K4me2_Rep2_20181202_icell8_225_(20181206_new_TGGGTTTCTTCGTGCA)


title: K562_H3K4me2_Rep2_20181202_icell8_224_(20181206_new_CAAGAATTCGGACTGC)


title: K562_H3K4me2_Rep2_20181202_icell8_223_(20181206_new_TCTTATTATCAAGAGC)


title: K562_H3K4me2_Rep2_20181202_icell8_222_(20181206_new_CGAGGCTGTCACATGG)


title: K562_H3K4me2_Rep2_20181202_icell8_246_(20181206_new_TCTTATTACCTGCGGG)


title: K562_H3K4me2_Rep2_20181202_icell8_220_(20181206_new_GAAATGCCTTGCCTAA)


title: K562_H3K4me2_Rep2_20181202_icell8_219_(20181206_new_CGAGGCTGTAAGTTAC)


title: K562_H3K4me2_Rep2_20181202_icell8_237_(20181206_new_TACTTGAAGTAAGGAG)


title: K562_H3K4me2_Rep2_20181202_icell8_218_(20181206_new_CGTACTAGGGTTCAAT)


title: K562_H3K4me2_Rep2_20181202_icell8_217_(20181206_new_GACTTAGTCGGACTGC)


title: K562_H3K4me2_Rep2_20181202_icell8_216_(20181206_new_TGGGTTTCATGGTCAT)


title: K562_H3K4me2_Rep2_20181202_icell8_221_(20181206_new_TTCCATATTCCGAGTT)


title: K562_H3K4me2_Rep2_20181202_icell8_215_(20181206_new_CGTACTAGCCACCGTT)


title: K562_H3K4me2_Rep2_20181202_icell8_213_(20181206_new_TAAGGCGAATCCAGGA)


title: K562_H3K4me2_Rep2_20181202_icell8_214_(20181206_new_CGAGGCTGCTCTCTAT)


title: K562_H3K4me2_Rep2_20181202_icell8_227_(20181206_new_TACTTGAAGATTCCCA)


title: K562_H3K4me2_Rep2_20181202_icell8_212_(20181206_new_ACCGGCTATCCATCAA)


title: K562_H3K4me2_Rep2_20181202_icell8_207_(20181206_new_ACCGGCTAATTACCGT)


title: K562_H3K4me2_Rep2_20181202_icell8_209_(20181206_new_CAAGAATTCGCCAGAT)


title: K562_H3K4me2_Rep2_20181202_icell8_208_(20181206_new_CATCCTGTAGTCCGAG)


title: K562_H3K4me2_Rep2_20181202_icell8_204_(20181206_new_CGCGGACACCACCGTT)


title: K562_H3K4me2_Rep2_20181202_icell8_202_(20181206_new_GTTAACAGTGATTGTT)


title: K562_H3K4me2_Rep2_20181202_icell8_211_(20181206_new_CCGTTTGTGATTCCCA)


title: K562_H3K4me2_Rep2_20181202_icell8_203_(20181206_new_GCTACGCTGAGACGTG)


title: K562_H3K4me2_Rep2_20181202_icell8_205_(20181206_new_GGTCATTTTACTGACC)


title: K562_H3K4me2_Rep2_20181202_icell8_206_(20181206_new_TTATGAAATTAACTTG)


title: K562_H3K4me2_Rep2_20181202_icell8_200_(20181206_new_TAAGGCGACTTTAACA)


title: K562_H3K4me2_Rep2_20181202_icell8_198_(20181206_new_CGTACTAGACCGGCCC)


title: K562_H3K4me2_Rep2_20181202_icell8_196_(20181206_new_TGGGTTTCCGTAGACC)


title: K562_H3K4me2_Rep2_20181202_icell8_199_(20181206_new_GGTAAACCGACGTGGC)


title: K562_H3K4me2_Rep2_20181202_icell8_195_(20181206_new_TTCCATATTGTTCCTG)


title: K562_H3K4me2_Rep2_20181202_icell8_194_(20181206_new_CCGTTTGTCCTTATGT)


title: K562_H3K4me2_Rep2_20181202_icell8_192_(20181206_new_TTATGAAAGGGCTTGG)


title: K562_H3K4me2_Rep2_20181202_icell8_210_(20181206_new_GATCCAAATTAACTTG)


title: K562_H3K4me2_Rep2_20181202_icell8_191_(20181206_new_GACTTAGTGCAAATTT)


title: K562_H3K4me2_Rep2_20181202_icell8_190_(20181206_new_ACAAAGTGGATCATTC)


title: K562_H3K4me2_Rep2_20181202_icell8_187_(20181206_new_CGGTTCCCCGCCAGAT)


title: K562_H3K4me2_Rep2_20181202_icell8_189_(20181206_new_CGTACTAGTAGATCGC)


title: K562_H3K4me2_Rep2_20181202_icell8_188_(20181206_new_TGGGTTTCTGATTGTT)


title: K562_H3K4me2_Rep2_20181202_icell8_193_(20181206_new_TTGCTAAGGGTTCAAT)


title: K562_H3K4me2_Rep2_20181202_icell8_186_(20181206_new_TAGGCATGACTTGTTG)


title: K562_H3K4me2_Rep2_20181202_icell8_201_(20181206_new_CAAGAATTTGGGCGGT)


title: K562_H3K4me2_Rep2_20181202_icell8_183_(20181206_new_GTTAACAGGATCATTC)


title: K562_H3K4me2_Rep2_20181202_icell8_182_(20181206_new_GGTCATTTTATCCTCT)


title: K562_H3K4me2_Rep2_20181202_icell8_179_(20181206_new_GTTAACAGCGGACTGC)


title: K562_H3K4me2_Rep2_20181202_icell8_184_(20181206_new_GGTCATTTGAGACGTG)


title: K562_H3K4me2_Rep2_20181202_icell8_197_(20181206_new_CTTGGGTCTGCATACT)


title: K562_H3K4me2_Rep2_20181202_icell8_180_(20181206_new_CCGTTTGTGCAAATTT)


title: K562_H3K4me2_Rep2_20181202_icell8_177_(20181206_new_ACAAAGTGTCCATCAA)


title: K562_H3K4me2_Rep2_20181202_icell8_178_(20181206_new_GGTCATTTATGGTCAT)


title: K562_H3K4me2_Rep2_20181202_icell8_175_(20181206_new_ACCGGCTACGATAGGG)


title: K562_H3K4me2_Rep2_20181202_icell8_173_(20181206_new_CGCGGACATATTTGCG)


title: K562_H3K4me2_Rep2_20181202_icell8_172_(20181206_new_GGACTCCTCGATAGGG)


title: K562_H3K4me2_Rep2_20181202_icell8_170_(20181206_new_CATCCTGTTAACCAAG)


title: K562_H3K4me2_Rep2_20181202_icell8_169_(20181206_new_TACTTGAAGGGCTTGG)


title: K562_H3K4me2_Rep2_20181202_icell8_185_(20181206_new_TACACCTCACTTGTTG)


title: K562_H3K4me2_Rep2_20181202_icell8_168_(20181206_new_ATTGCCGGACGATAAC)


title: K562_H3K4me2_Rep2_20181202_icell8_167_(20181206_new_TTCCATATACCGGCCC)


title: K562_H3K4me2_Rep2_20181202_icell8_181_(20181206_new_TTCCATATGGTGAAGG)


title: K562_H3K4me2_Rep2_20181202_icell8_160_(20181206_new_GGACTCCTACGATAAC)


title: K562_H3K4me2_Rep2_20181202_icell8_165_(20181206_new_CATCCTGTCTTTAACA)


title: K562_H3K4me2_Rep2_20181202_icell8_162_(20181206_new_GGTCATTTGTAAGGAG)


title: K562_H3K4me2_Rep2_20181202_icell8_164_(20181206_new_TCTTATTACAGCAACG)


title: K562_H3K4me2_Rep2_20181202_icell8_163_(20181206_new_TACACCTCAGCCGTTC)


title: K562_H3K4me2_Rep2_20181202_icell8_159_(20181206_new_CAAGAATTGATCATTC)


title: K562_H3K4me2_Rep2_20181202_icell8_158_(20181206_new_CGTACTAGACTGCATA)


title: K562_H3K4me2_Rep2_20181202_icell8_157_(20181206_new_AATGGTAGATTACCGT)


title: K562_H3K4me2_Rep2_20181202_icell8_171_(20181206_new_CGCGGACACGCCAGAT)


title: K562_H3K4me2_Rep2_20181202_icell8_154_(20181206_new_CATCCTGTTTTCTAGC)


title: K562_H3K4me2_Rep2_20181202_icell8_155_(20181206_new_TCTTATTAACTGCATA)


title: K562_H3K4me2_Rep2_20181202_icell8_176_(20181206_new_CGAGGCTGTTTCTAGC)


title: K562_H3K4me2_Rep2_20181202_icell8_152_(20181206_new_CATCCTGTACTGCATA)


title: K562_H3K4me2_Rep2_20181202_icell8_151_(20181206_new_CTTGGGTCACATTCGT)


title: K562_H3K4me2_Rep2_20181202_icell8_149_(20181206_new_GGTCATTTGGTTCAAT)


title: K562_H3K4me2_Rep2_20181202_icell8_148_(20181206_new_GGTAAACCACTGCATA)


title: K562_H3K4me2_Rep2_20181202_icell8_150_(20181206_new_GGTAAACCGGCACGGA)


title: K562_H3K4me2_Rep2_20181202_icell8_156_(20181206_new_AATGGTAGTGATTGTT)


title: K562_H3K4me2_Rep2_20181202_icell8_153_(20181206_new_GGTAAACCATTGGGTC)


title: K562_H3K4me2_Rep2_20181202_icell8_146_(20181206_new_TCTTATTACGGACTGC)


title: K562_H3K4me2_Rep2_20181202_icell8_145_(20181206_new_CGCGGACATGTTCCTG)


title: K562_H3K4me2_Rep2_20181202_icell8_166_(20181206_new_TTCCATATTTCGTGCA)


title: K562_H3K4me2_Rep2_20181202_icell8_161_(20181206_new_TTCCATATTAAGTTAC)


title: K562_H3K4me2_Rep2_20181202_icell8_147_(20181206_new_GGTAAACCAGTCCGAG)


title: K562_H3K4me2_Rep2_20181202_icell8_144_(20181206_new_GGTAAACCAGAGTAGA)


title: K562_H3K4me2_Rep2_20181202_icell8_143_(20181206_new_TACTTGAATAAGTTAC)


title: K562_H3K4me2_Rep2_20181202_icell8_142_(20181206_new_ACCGGCTACTAAGCCT)


title: K562_H3K4me2_Rep2_20181202_icell8_139_(20181206_new_GTGTGGTGCAGCAACG)


title: K562_H3K4me2_Rep2_20181202_icell8_140_(20181206_new_CGGCCAATCTCTCTAT)


title: K562_H3K4me2_Rep2_20181202_icell8_136_(20181206_new_TAAGGCGAAATCTACA)


title: K562_H3K4me2_Rep2_20181202_icell8_138_(20181206_new_CTTGGGTCATGGTCAT)


title: K562_H3K4me2_Rep2_20181202_icell8_137_(20181206_new_GTTAACAGCTTTAACA)


title: K562_H3K4me2_Rep2_20181202_icell8_135_(20181206_new_GATCCAAAAAGGAGTA)


title: K562_H3K4me2_Rep2_20181202_icell8_141_(20181206_new_GGAGTAAGGTGTATCG)


title: K562_H3K4me2_Rep2_20181202_icell8_133_(20181206_new_GGTCATTTAACATGAT)


title: K562_H3K4me2_Rep2_20181202_icell8_134_(20181206_new_GGGAATGACTTTAACA)


title: K562_H3K4me2_Rep2_20181202_icell8_132_(20181206_new_TGGATCTGAGAGATCT)


title: K562_H3K4me2_Rep2_20181202_icell8_131_(20181206_new_TTCCATATACGCGCAG)


title: K562_H3K4me2_Rep2_20181202_icell8_130_(20181206_new_TGGGTTTCGTGTATCG)


title: K562_H3K4me2_Rep2_20181202_icell8_129_(20181206_new_TGGATCTGCGGACTGC)


title: K562_H3K4me2_Rep2_20181202_icell8_122_(20181206_new_CGAGGCTGGGTTCAAT)


title: K562_H3K4me2_Rep2_20181202_icell8_125_(20181206_new_GGACTCCTGATCATTC)


title: K562_H3K4me2_Rep2_20181202_icell8_127_(20181206_new_CAAGAATTGATTCCCA)


title: K562_H3K4me2_Rep2_20181202_icell8_126_(20181206_new_AATGGTAGGGCACGGA)


title: K562_H3K4me2_Rep2_20181202_icell8_124_(20181206_new_CGGCCAATAGAGATCT)


title: K562_H3K4me2_Rep2_20181202_icell8_128_(20181206_new_CGTACTAGTCCGAGTT)


title: K562_H3K4me2_Rep2_20181202_icell8_119_(20181206_new_TTATGAAATGCATACT)


title: K562_H3K4me2_Rep2_20181202_icell8_123_(20181206_new_CGCGGACAACTTGTTG)


title: K562_H3K4me2_Rep2_20181202_icell8_121_(20181206_new_GGAGTAAGTGTTCCTG)


title: K562_H3K4me2_Rep2_20181202_icell8_120_(20181206_new_TTCCATATAGAGTAGA)


title: K562_H3K4me2_Rep2_20181202_icell8_116_(20181206_new_CGAGGCTGCCAGTTTA)


title: K562_H3K4me2_Rep2_20181202_icell8_118_(20181206_new_CGAGGCTGTTCGTGCA)


title: K562_H3K4me2_Rep2_20181202_icell8_117_(20181206_new_ACCGGCTACCAGTTTA)


title: K562_H3K4me2_Rep2_20181202_icell8_115_(20181206_new_TAACAACATACTGACC)


title: K562_H3K4me2_Rep2_20181202_icell8_112_(20181206_new_GTGTGGTGGGGCTTGG)


title: K562_H3K4me2_Rep2_20181202_icell8_111_(20181206_new_CGGTTCCCAGAGATCT)


title: K562_H3K4me2_Rep2_20181202_icell8_114_(20181206_new_GGTAAACCTACTGACC)


title: K562_H3K4me2_Rep2_20181202_icell8_110_(20181206_new_TAGGCATGCTTTAACA)


title: K562_H3K4me2_Rep2_20181202_icell8_109_(20181206_new_TTATGAAATGTTCCTG)


title: K562_H3K4me2_Rep2_20181202_icell8_107_(20181206_new_TGGATCTGCTTTAACA)


title: K562_H3K4me2_Rep2_20181202_icell8_105_(20181206_new_TTGCTAAGAAGGAGTA)


title: K562_H3K4me2_Rep2_20181202_icell8_106_(20181206_new_GGTAAACCACATTCGT)


title: K562_H3K4me2_Rep2_20181202_icell8_104_(20181206_new_CCGTTTGTCGTACCAA)


title: K562_H3K4me2_Rep2_20181202_icell8_103_(20181206_new_TCTTATTACTAGAAGT)


title: K562_H3K4me2_Rep2_20181202_icell8_101_(20181206_new_CGAGGCTGAAGGAGTA)


title: K562_H3K4me2_Rep2_20181202_icell8_108_(20181206_new_ACAAAGTGTTCGTGCA)


title: K562_H3K4me2_Rep2_20181202_icell8_113_(20181206_new_ACAAAGTGGCAGCCTC)


title: K562_H3K4me2_Rep2_20181202_icell8_102_(20181206_new_TGGATCTGGCAGCCTC)


title: K562_H3K4me2_Rep2_20181202_icell8_96_(20181206_new_GATCCAAAGTCGGACT)


title: K562_H3K4me2_Rep2_20181202_icell8_97_(20181206_new_GATCCAAAATGGTCAT)


title: K562_H3K4me2_Rep2_20181202_icell8_100_(20181206_new_GTTAACAGACGATAAC)


title: K562_H3K4me2_Rep2_20181202_icell8_87_(20181206_new_GACTTAGTTTCGTGCA)


title: K562_H3K4me2_Rep2_20181202_icell8_90_(20181206_new_TAGGCATGTCAAGAGC)


title: K562_H3K4me2_Rep2_20181202_icell8_99_(20181206_new_CGCGGACACGGACTGC)


title: K562_H3K4me2_Rep2_20181202_icell8_83_(20181206_new_GCTACGCTACGATAAC)


title: K562_H3K4me2_Rep2_20181202_icell8_94_(20181206_new_TACTTGAATGTTCCTG)


title: K562_H3K4me2_Rep2_20181202_icell8_88_(20181206_new_CGGCCAATCTTTAACA)


title: K562_H3K4me2_Rep2_20181202_icell8_95_(20181206_new_CGAGGCTGGGGCTTGG)


title: K562_H3K4me2_Rep2_20181202_icell8_85_(20181206_new_TAACAACAGGGCTTGG)


title: K562_H3K4me2_Rep2_20181202_icell8_82_(20181206_new_TTGCTAAGGAGACGTG)


title: K562_H3K4me2_Rep2_20181202_icell8_174_(20181206_new_TCTTATTATAAGTTAC)


title: K562_H3K4me2_Rep2_20181202_icell8_93_(20181206_new_TACACCTCGACGTGGC)


title: K562_H3K4me2_Rep2_20181202_icell8_84_(20181206_new_CGTACTAGACTTGTTG)


title: K562_H3K4me2_Rep2_20181202_icell8_81_(20181206_new_AATGGTAGACATTCGT)


title: K562_H3K4me2_Rep2_20181202_icell8_80_(20181206_new_ATTGCCGGGCATTAAG)


title: K562_H3K4me2_Rep2_20181202_icell8_79_(20181206_new_ACCGGCTAGAGACGTG)


title: K562_H3K4me2_Rep2_20181202_icell8_78_(20181206_new_TTATGAAAACTTGTTG)


title: K562_H3K4me2_Rep2_20181202_icell8_98_(20181206_new_CGTACTAGGTAAGGAG)


title: K562_H3K4me2_Rep2_20181202_icell8_91_(20181206_new_CTTGGGTCATCCAGGA)


title: K562_H3K4me2_Rep2_20181202_icell8_92_(20181206_new_TGGGTTTCCTTTAACA)


title: K562_H3K4me2_Rep2_20181202_icell8_77_(20181206_new_CGGTTCCCAACATGAT)


title: K562_H3K4me2_Rep2_20181202_icell8_76_(20181206_new_TACTTGAACTTTAACA)


title: K562_H3K4me2_Rep2_20181202_icell8_89_(20181206_new_GACTTAGTGAACTCGA)


title: K562_H3K4me2_Rep2_20181202_icell8_86_(20181206_new_ACCGGCTAACGCGCAG)


title: K562_H3K4me2_Rep2_20181202_icell8_74_(20181206_new_TACACCTCTTCGTGCA)


title: K562_H3K4me2_Rep2_20181202_icell8_72_(20181206_new_GGTAAACCTGATGAAA)


title: K562_H3K4me2_Rep2_20181202_icell8_73_(20181206_new_GACTTAGTGTCGGACT)


title: K562_H3K4me2_Rep2_20181202_icell8_69_(20181206_new_ACCGGCTAAAGGAGTA)


title: K562_H3K4me2_Rep2_20181202_icell8_70_(20181206_new_CGGCCAATTAAGTTAC)


title: K562_H3K4me2_Rep2_20181202_icell8_66_(20181206_new_ACAAAGTGGCATTAAG)


title: K562_H3K4me2_Rep2_20181202_icell8_71_(20181206_new_TAAGGCGACCTGCGGG)


title: K562_H3K4me2_Rep2_20181202_icell8_65_(20181206_new_CAAGAATTAGTCCGAG)


title: K562_H3K4me2_Rep2_20181202_icell8_67_(20181206_new_TAGGCATGTTTCTAGC)


title: K562_H3K4me2_Rep2_20181202_icell8_68_(20181206_new_GCTACGCTACTGCATA)


title: K562_H3K4me2_Rep2_20181202_icell8_64_(20181206_new_CAAGAATTTAACCAAG)


title: K562_H3K4me2_Rep2_20181202_icell8_75_(20181206_new_GATCCAAAACTTGTTG)


title: K562_H3K4me2_Rep2_20181202_icell8_62_(20181206_new_GGTAAACCACTTGTTG)


title: K562_H3K4me2_Rep2_20181202_icell8_63_(20181206_new_GGAGTAAGGTAAGGAG)


title: K562_H3K4me2_Rep2_20181202_icell8_60_(20181206_new_GAAATGCCTGTTCCTG)


title: K562_H3K4me2_Rep2_20181202_icell8_56_(20181206_new_CATCCTGTTTAACTTG)


title: K562_H3K4me2_Rep2_20181202_icell8_57_(20181206_new_GATCCAAAAACATGAT)


title: K562_H3K4me2_Rep2_20181202_icell8_55_(20181206_new_GGGAATGAGGTTCAAT)


title: K562_H3K4me2_Rep2_20181202_icell8_54_(20181206_new_TTCCATATATCCAGGA)


title: K562_H3K4me2_Rep2_20181202_icell8_53_(20181206_new_GGGAATGATATCCTCT)


title: K562_H3K4me2_Rep2_20181202_icell8_52_(20181206_new_GCTACGCTGATCATTC)


title: K562_H3K4me2_Rep2_20181202_icell8_51_(20181206_new_CGGTTCCCAGTCCGAG)


title: K562_H3K4me2_Rep2_20181202_icell8_48_(20181206_new_GGAGTAAGGGTGAAGG)


title: K562_H3K4me2_Rep2_20181202_icell8_50_(20181206_new_TAGGCATGGCATTAAG)


title: K562_H3K4me2_Rep2_20181202_icell8_49_(20181206_new_TGGGTTTCGCATTAAG)


title: K562_H3K4me2_Rep2_20181202_icell8_47_(20181206_new_GATCCAAATAACCAAG)


title: K562_H3K4me2_Rep2_20181202_icell8_61_(20181206_new_TTATGAAAAGGTTGCC)


title: K562_H3K4me2_Rep2_20181202_icell8_46_(20181206_new_ATTGCCGGAACATGAT)


title: K562_H3K4me2_Rep2_20181202_icell8_45_(20181206_new_TAACAACAAATCTACA)


title: K562_H3K4me2_Rep2_20181202_icell8_42_(20181206_new_GGTCATTTCGTACCAA)


title: K562_H3K4me2_Rep2_20181202_icell8_43_(20181206_new_GTTAACAGAGGTTGCC)


title: K562_H3K4me2_Rep2_20181202_icell8_41_(20181206_new_ATTGCCGGGATCATTC)


title: K562_H3K4me2_Rep2_20181202_icell8_58_(20181206_new_TCTTATTAAATCTACA)


title: K562_H3K4me2_Rep2_20181202_icell8_40_(20181206_new_TGGATCTGTAGATCGC)


title: K562_H3K4me2_Rep2_20181202_icell8_44_(20181206_new_GTGTGGTGCGATAGGG)


title: K562_H3K4me2_Rep2_20181202_icell8_59_(20181206_new_ATTGCCGGGTCGGACT)


title: K562_H3K4me2_Rep2_20181202_icell8_37_(20181206_new_TAGGCATGATTACCGT)


title: K562_H3K4me2_Rep2_20181202_icell8_32_(20181206_new_GGGAATGATCACATGG)