title: D14-401037

description:
muscle cell by qPCR collagen expression
140929Red_D14-401037
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401099

status: Public on May 25 2019

type: SRA

city: Cambridge

first: Peter

middle: W

last: Reddien

state: MA

address: 9 Cambridge Center

country: USA

institute: Whitehead Institute for Biomedical Research

description:
muscle cell by qPCR collagen expression
140929Red_D14-401099
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt

library_source: transcriptomic

data_processing:
Basecalls performed using CASAVA version 1.7.0.
Reads were trimmed using Trimmomatic-0.32 to remove Nextera transposon sequences and low quality 3′ base pairs.
Reads were mapped to dd_Smed_v4 planarian transcriptome assembly using bowtie 1 with --best to generate raw read counts file.
Only cells with a total read count of >10,000 from all genes were included in subsequent analysis. Read counts were normalized to cpm.
Cells with collagen (dd_Smed_v4_702_0_1) cpm <40, troponin (dd_Smed_V4_323_0_2) cpm <80, and with tropomyosin (dd_Smed_v4_436_0_1) cpm of 0 were excluded from analysis
Cells with high AGAT expression were excluded from analysis
Reads for isotigs of contigs (all contig ids that vary only by the final number) were summed.
Reads for mitochondrial and ribosomal RNA (dd_Smed_v4_7_0, and dd_Smed_v4_4_1, mtRNA, rRNA_5s) were discarded.
Differential gene expression analysis was performed between posterior pole cells (11 total) and non-posterior-pole muscle cells (90 total) using SCDE analysis.
Genome_build: dd_Smed_v4
Supplementary_files_format_and_content: Raw read counts: Tab-delineated text file includes unnormalized read counts of contigs for all 152 cells. SCDE analyses: tab-delineated text file generated per Kharchenko et al, 2014 for differential gene expression between posterior pole cells (11 total) and non-posterior-pole muscle cells (90 total) that passing quality filters (per data processing steps delineated above). Fold change is indicated as the maximum likelihood estimate (mle) with a lower (lb) and upper bound (ub) and a conservative estimate (ce, equal to lb (positive), ub (negative) or 0 (lb and ub have different signs)) and statistical significance as signed Z-score (Z) and conservative Z-score (cZ, Z score corrected for multiple hypothesis using Holm procedure).

molecule: polyA RNA

organism: Schmidtea mediterranea

growth_protocol: Asexual Schmidtea mediterranea strain (CIW4) animals starved 7-14 days before surgical resection of midline tail tip regions, or before transverse tail amputation, then resection of midline posterior blastema regions after 72 hours of regeneration.

extract_protocol:
Cells were lysed in total cell lysis buffer supplemented with 1% β-mercaptoethanol
cDNA and sequencing libraries were constructed using SmartSeq2.

treatment_protocol: Single cell suspensions for each region were stained with Hoechst, and non-dividing single cells were sorted by flow cytometry into 96 well plates

tag: strain

value: asexual CIW4

tag: ploidy

value: 2N (non-dividing)

tag: uninjured or regenerating animal

value: regenerating animal 72 hours post transverse amputation

tag: cell type

value: non-pole muscle cell


title: D14-401036

description:
muscle cell by qPCR collagen expression
140929Red_D14-401036
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401034

description:
muscle cell by qPCR collagen expression
140929Red_D14-401034
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401035

description:
muscle cell by qPCR collagen expression
140929Red_D14-401035
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401030

description:
muscle cell by qPCR collagen expression
140929Red_D14-401030
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401032

description:
muscle cell by qPCR collagen expression
140929Red_D14-401032
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401029

description:
muscle cell by qPCR collagen expression
140929Red_D14-401029
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401033

description:
muscle cell by qPCR collagen expression
140929Red_D14-401033
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401031

description:
muscle cell by qPCR collagen expression
140929Red_D14-401031
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401040

description:
muscle cell by qPCR collagen expression
140929Red_D14-401040
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401028

description:
muscle cell by qPCR collagen expression
140929Red_D14-401028
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401026

description:
muscle cell by qPCR collagen expression
140929Red_D14-401026
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401027

description:
muscle cell by qPCR collagen expression
140929Red_D14-401027
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401025

description:
muscle cell by qPCR collagen expression
140929Red_D14-401025
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401049

description:
muscle cell by qPCR collagen expression
140929Red_D14-401049
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt

value: uninjured


title: D14-401021

description:
muscle cell by qPCR collagen expression
140929Red_D14-401021
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401024

description:
muscle cell by qPCR collagen expression
140929Red_D14-401024
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401022

description:
muscle cell by qPCR collagen expression
140929Red_D14-401022
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401023

description:
muscle cell by qPCR collagen expression
140929Red_D14-401023
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401019

description:
muscle cell by qPCR collagen expression
140929Red_D14-401019
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401017

description:
muscle cell by qPCR collagen expression
140929Red_D14-401017
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401018

description:
muscle cell by qPCR collagen expression
140929Red_D14-401018
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401020

description:
muscle cell by qPCR collagen expression
140929Red_D14-401020
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401015

description:
muscle cell by qPCR collagen expression
140929Red_D14-401015
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401016

description:
muscle cell by qPCR collagen expression
140929Red_D14-401016
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401013

description:
muscle cell by qPCR collagen expression
140929Red_D14-401013
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401014

description:
muscle cell by qPCR collagen expression
140929Red_D14-401014
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401012

description:
muscle cell by qPCR collagen expression
140929Red_D14-401012
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401011

description:
muscle cell by qPCR collagen expression
140929Red_D14-401011
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401007

description:
muscle cell by qPCR collagen expression
140929Red_D14-401007
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401008

description:
muscle cell by qPCR collagen expression
140929Red_D14-401008
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401010

description:
muscle cell by qPCR collagen expression
140929Red_D14-401010
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401006

description:
muscle cell by qPCR collagen expression
140929Red_D14-401006
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401005

description:
muscle cell by qPCR collagen expression
140929Red_D14-401005
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401009

description:
muscle cell by qPCR collagen expression
140929Red_D14-401009
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401003

description:
muscle cell by qPCR collagen expression
140929Red_D14-401003
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401004

description:
muscle cell by qPCR collagen expression
140929Red_D14-401004
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401001

description:
muscle cell by qPCR collagen expression
140929Red_D14-401001
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-400999

description:
muscle cell by qPCR collagen expression
140929Red_D14-400999
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-400998

description:
muscle cell by qPCR collagen expression
140929Red_D14-400998
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-400995

description:
muscle cell by qPCR collagen expression
140929Red_D14-400995
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401000

description:
muscle cell by qPCR collagen expression
140929Red_D14-401000
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-400994

description:
muscle cell by qPCR collagen expression
140929Red_D14-400994
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-400992

description:
muscle cell by qPCR collagen expression
140929Red_D14-400992
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-400989

description:
muscle cell by qPCR collagen expression
140929Red_D14-400989
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-400991

description:
muscle cell by qPCR collagen expression
140929Red_D14-400991
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-400986

description:
muscle cell by qPCR collagen expression
140929Red_D14-400986
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-400987

description:
muscle cell by qPCR collagen expression
140929Red_D14-400987
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-400983

description:
muscle cell by qPCR collagen expression
140929Red_D14-400983
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-400985

description:
muscle cell by qPCR collagen expression
140929Red_D14-400985
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt

source_name: uninjured_pole_Isolated planarian cell

value: pole muscle cell


title: D14-400990

description:
muscle cell by qPCR collagen expression
140929Red_D14-400990
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-400988

description:
muscle cell by qPCR collagen expression
140929Red_D14-400988
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-400982

description:
muscle cell by qPCR collagen expression
140929Red_D14-400982
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-400984

description:
muscle cell by qPCR collagen expression
140929Red_D14-400984
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-400978

description:
muscle cell by qPCR collagen expression
140929Red_D14-400978
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-400981

description:
muscle cell by qPCR collagen expression
140929Red_D14-400981
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-400980

description:
muscle cell by qPCR collagen expression
140929Red_D14-400980
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-400975

description:
muscle cell by qPCR collagen expression
140929Red_D14-400975
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-400977

description:
muscle cell by qPCR collagen expression
140929Red_D14-400977
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-401002

description:
muscle cell by qPCR collagen expression
140929Red_D14-401002
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-400979

description:
muscle cell by qPCR collagen expression
140929Red_D14-400979
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-400976

description:
muscle cell by qPCR collagen expression
140929Red_D14-400976
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-400973

description:
muscle cell by qPCR collagen expression
140929Red_D14-400973
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-400972

description:
muscle cell by qPCR collagen expression
140929Red_D14-400972
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-400974

description:
muscle cell by qPCR collagen expression
140929Red_D14-400974
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-400996

description:
muscle cell by qPCR collagen expression
140929Red_D14-400996
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-400997

description:
muscle cell by qPCR collagen expression
140929Red_D14-400997
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-400993

description:
muscle cell by qPCR collagen expression
140929Red_D14-400993
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: D14-400967

description:
muscle cell by qPCR collagen expression
140929Red_D14-400967
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: input_rep2

city: Stanford

first: Cheen Euong

last: Ang

state: CA

address: 265 Campus Drive, G3145

institute: Stanford University

library_source: genomic

data_processing:
Library strategy: ChIRP-sequencing
Peaks were called from the merged tracks over RNase control tracks using MACS14.
Overlapping peaks from all replicates were final peaks. High confidence peaks were then filtered by their significance [−log10 (p-value) ≥100] and correlation between even/odd probes >0, average coverage >1 for hNPC.
Genome_build: hg19
Supplementary_files_format_and_content: txt file containing the peak lists obtained.

molecule: genomic DNA

organism: Homo sapiens

source_name: Neuronal progenitor cells

growth_protocol: The hESC was seeded and differentiated in N2B27 media with 0.1mM of ascorbic acid with dual smad (LDN and SB431542) for six days into neural progenitor cells (NPC) according to Du Nature Communication 2015.

extract_protocol:
RNase pre-treated samples are served as negative controls for probe-DNA hybridization. Independent even and odd probe pools were used to ensure concordant lncRNA-specific retrieval. Even and odd labels in the sequencing files correspond to the pulldown using the two different pools of probes (even and odd).
ChIRP libraries are constructed using the NEBNext DNA library preparation kit.

treatment_protocol: The cells are trypsinized and fixed with 1% Glutaraldehyde.

value: Day 6 in vitro differentiated NPC


title: D14-400971

description:
muscle cell by qPCR collagen expression
140929Red_D14-400971
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: short_odd_Rnase_rep2


title: D14-400969

description:
muscle cell by qPCR collagen expression
140929Red_D14-400969
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: short_even_Rnase_rep2


title: short_odd_rep2


title: short_even_rep2


title: long_even_rep2


title: long_odd_Rnase_rep2


title: short_odd_Rnase_rep1


title: short_even_Rnase_rep1


title: short_even_rep1


title: long_odd_rep2


title: input_rep1


title: short_odd_rep1


title: long_odd_rep1


title: long_even_Rnase_rep1


title: long_odd_Rnase_rep1


title: D14-400968

description:
muscle cell by qPCR collagen expression
140929Red_D14-400968
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: mESiN_odd_rep1

data_processing:
Library strategy: ChIRP-sequencing
Peaks were called from the merged tracks over RNase control tracks using MACS14.
Overlapping peaks from all replicates were final peaks. High confidence peaks were then by their significance [−log10 (p-value) ≥100] and correlation between even/odd probes >0, average coverage (>2 for mES-iN).
Genome_build: mm9
Supplementary_files_format_and_content: txt file containing the peak lists obtained.

organism: Mus musculus

source_name: mouse embryonic stem cells derived induced neurons (mESiN)

growth_protocol: Mouse Embyornic Stem Cells (mES) were cultured in mES media.

extract_protocol:
The cells are trypsinized and fixed with 3% formaldehyde. RNase pre-treated samples are served as negative controls for probe-DNA hybridization. Independent even and odd probe pools were used to ensure concordant lncRNA-specific retrieval. Even and odd labels in the sequencing files correspond to the pulldown using the two different pools of probes (even and odd).
ChIRP libraries are constructed using the NEBNext DNA library preparation kit.

treatment_protocol: The mES cells were infected with FUW-rtTA and TetO-Ngn2-T2A-puro. The media were changed to neuronal media (DMEM/F12 + 1% Pen/Strep +  1.6ml Insulin + N2 + B27) with dox the next day and cultured for another 4 days to generate induced neurons (iN).

value: Day 4 Embryonic Stem Cell derived induced neurons


title: long_even_rep1


title: D14-400970

description:
muscle cell by qPCR collagen expression
140929Red_D14-400970
processed data file; raw read counts:
ReadcountMaster_uniquegenes2_forGEO_onlySinglecells.txt
processed data file; differential expression analysis:
ppolecandidates_singlecell_PvsNP_modelbygroup_forGEO.txt


title: mESiN_odd_Rnase_rep1


title: mESiN_odd_Rnase_rep2


title: mESiN_odd_rep2


title: mESiN_even_rep1


title: mESiN_even_rep2


title: mESiN_even_Rnase_rep2


title: long_even_Rnase_rep2


title: mESiN_input_rep1


title: mESiN_even_Rnase_rep1


title: 7811210082_R06C01

type: genomic

city: Hefei

first: Chao

last: Yang

address: #69 Meishan Road, Hefei230032, Anhui, China

country: China

institute: First Affiliated Hospital of Anhui Medical University

hyb_protocol: The whole genome genotyping was conducted at Chinese National Human Genome  Center at Shanghai, China. Approximately 200ng of genomic DNA was used for  genotyping analysis. Briefly, each sample was whole-genome amplified, fragmented,  precipitated and resuspended in appropriate hybridization buffer.

scan_protocol: After  hybridization, the BeadChip oligonucleotides were extended by a single labeled base,  which was detected by fluorescence imaging with an Illumina Bead Array Reader.

data_processing:
Genomic DNA extracted from whole blood was genotyped using Illumina Human Exome Fine Mapping BeadChip (Illumina).
Processed data filde includes: Genotype: AA,AB,BB,or NC (no call), GC Score, Theta, R, B Allele Freq, Log R Ratio

label: not provided

source_name: Public control

label_protocol: not provided

extract_protocol: Genomic DNA was extracted from peripheral blood lymphocytes by standard  procedures (Miller et al, 1988) using Flexi Gene DNA kits (QIAGEN, Germany)

tag: gender

value: Unknown

tag: tissue

value: Whole blood

tag: disease state

value: healthy control


title: 7811210067_R02C01

description: Genomic DNA extracted from whole blood was genotyped using Illumina Human Exome Fine Mapping BeadChip (Illumina).


title: 7811210067_R01C01


title: 7811210067_R05C02


title: 7811210067_R03C02


title: 7811210082_R03C02


title: 7811210082_R05C01


title: 7811210067_R04C01


title: 7811210067_R06C01


title: 7811210082_R04C02


title: 7811210067_R03C01


title: 7811210067_R02C02


title: 7811210082_R02C02


title: 7811210067_R05C01


title: 7811210082_R05C02


title: 7811210082_R03C01


title: 7811210067_R04C02


title: 7811210082_R04C01


title: 7811210082_R02C01


title: 7811210082_R01C02


title: 7811210066_R05C02


title: 7811210066_R06C02


title: 7811210082_R01C01


title: 7811210082_R06C02


title: 7811210066_R04C02


title: 7811210067_R01C02


title: 7811210066_R03C01


title: 7811210066_R02C02


title: 7811210066_R03C02


title: 7811210066_R01C01


title: 7811210073_R05C02


title: 7811210066_R02C01


title: 7811210073_R04C01


title: 7811210073_R06C02


title: 7811210073_R04C02


title: 7811210066_R01C02


title: 7811210073_R03C01


title: 7811210073_R05C01


title: 7811210073_R02C02


title: 7811210073_R02C01


title: 7811210073_R03C02


title: 7811210087_R04C01


title: 7811210087_R04C02


title: 7811210073_R01C01


title: 7811210087_R05C02


title: 7811210073_R01C02


title: 7811210087_R06C01


title: 7811210087_R05C01


title: 7811210087_R06C02


title: 7811210087_R03C02


title: 7811210087_R03C01


title: 7811210087_R02C02


title: 7811210087_R01C01


title: 7811210087_R02C01


title: 7811210073_R06C01


title: 7811210063_R05C01


title: 7811210063_R03C02


title: 7811210063_R02C01


title: 7811210063_R04C01


title: 7811210063_R01C02


title: 7811210063_R03C01


title: 7811210090_R06C02


title: 7811210063_R04C02


title: 7811210063_R02C02


title: 7811210090_R05C02


title: 7811210063_R01C01


title: 7811210090_R03C01


title: 7811210090_R06C01


title: 7811210090_R02C02


title: 7811210090_R05C01


title: 7811210085_R06C02


title: 7811210090_R04C02


title: 7811210090_R03C02


title: 7811210090_R01C02


title: 7811210085_R05C02


title: 7811210090_R02C01


title: 7811210087_R01C02


title: 7811210085_R04C02


title: 7811210085_R05C01


title: 7811210085_R04C01


title: 7811210085_R06C01


title: 7811210085_R03C02


title: 7811210085_R03C01


title: 7811210090_R04C01


title: 7811210085_R02C02


title: 7825611074_R01C01

source_name: Psoriasis


title: 7817179145_R06C02


title: 7811210090_R01C01


title: 7817179145_R04C02


title: 7817179145_R01C02


title: 7817179145_R03C02


title: 7817179145_R04C01


title: 7817179145_R01C01


title: 7811244007_R06C02


title: 7817179145_R05C01


title: 7817179145_R06C01


title: 7817179145_R02C02


title: 7817179145_R03C01


title: 7825611074_R01C02


title: 7811244007_R04C02


title: 7817179145_R02C01


title: 7811244007_R06C01


title: 7811244007_R05C01


title: 7811244007_R03C02


title: 7811244007_R03C01


title: 7811244007_R04C01


title: 7811244007_R01C01


title: 7811244007_R02C01


title: 7817179145_R05C02


title: 7811244021_R06C02


title: 7811244021_R06C01


title: 7811244021_R05C02


title: 7811244007_R05C02


title: 7811244007_R01C02


title: 7811244021_R04C01


title: 7811244021_R05C01


title: 7811244021_R04C02


title: 7811244007_R02C02


title: 7811244021_R03C01


title: 7811244021_R02C01


title: 7811244021_R01C02


title: 7811244089_R06C02


title: 7811244089_R02C01


title: 7811244021_R02C02


title: 7811244021_R01C01


title: 7811244089_R04C02


title: 7811244089_R05C01


title: 7811244089_R01C02


title: 7811244089_R02C02


title: 7811244089_R03C02


title: 7811244021_R03C02


title: 7811244089_R04C01


title: 7811244089_R06C01


title: 7811244089_R05C02


title: 7825611090_R06C01


title: 7825611090_R05C01


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title: 7825611090_R04C02


title: 7825611090_R03C02


title: 7825611090_R04C01


title: 7825611090_R02C01


title: 7825611090_R02C02


title: 7825611090_R01C02


title: 7825611090_R01C01


title: 7811244089_R03C01


title: 7825611067_R06C01


title: 7825611067_R06C02


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title: 7825611035_R03C01


title: 7825611035_R02C02


title: 7825611067_R01C01


title: 7825611035_R05C02


title: 7825611067_R03C02


title: 7825611035_R03C02


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title: 7811244140_R05C01


title: 7825611067_R04C01


title: 7825611067_R04C02


title: 7825611067_R02C02


title: 7825611035_R06C02


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title: 7825611035_R06C01


title: 7811244140_R03C01


title: 7811244140_R03C02


title: 7825611035_R04C02


title: 7811244140_R01C02


title: 7811244140_R04C01


title: 7817136112_R06C02


title: 7817136112_R04C01


title: 7811244140_R01C01


title: 7811244140_R02C01


title: 7817136112_R04C02


title: 7817136112_R05C02


title: 7817136112_R06C01


title: 7811244140_R06C01


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title: 7817136112_R03C02


title: 7811244140_R06C02


title: 7817136112_R02C01


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title: 7811261067_R06C01


title: 7811261067_R04C02


title: 7811261067_R05C01


title: 7811261067_R03C02


title: 7811261067_R04C01


title: 7811261067_R01C02


title: 7811261067_R06C02


title: 7811261067_R03C01


title: 7811261067_R02C02


title: 7817136112_R01C02


title: 7811261067_R02C01


title: 7825611026_R04C02


title: 7825611026_R05C02


title: 7825611026_R06C01


title: 7825611026_R05C01


title: 7825611026_R06C02


title: 7811261067_R01C01


title: 7825611026_R02C02


title: 7825611026_R03C01


title: 7825611026_R01C02


title: 7825611026_R01C01


title: 7825611026_R03C02


title: 7817136112_R01C01


title: 7825611026_R04C01


title: 7825611026_R02C01


title: 7817128107_R06C01


title: 7811261067_R05C02


title: 7817128107_R05C01


title: 7817128107_R06C02


title: 7817128107_R05C02


title: 7817128107_R04C02


title: 7817128107_R02C01


title: 7817128107_R02C02


title: 7817128107_R03C02


title: 7817128107_R01C01


title: 7817128107_R04C01


title: 7817128107_R03C01


title: 7811252056_R06C01


title: 7811252056_R06C02


title: 7817128107_R01C02


title: 7811252056_R04C02


title: 7811252056_R03C01


title: 7811252056_R03C02


title: 7811252056_R04C01


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title: 7811252056_R01C01


title: 7811244099_R05C02


title: 7811252056_R02C01


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title: 7811244099_R06C01


title: 7811244099_R04C01


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title: 7811244099_R06C02


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title: 7817144041_R06C02


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title: 7825611080_R06C02


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title: 7817179133_R01C01


title: 7825611080_R05C01


title: 7825611080_R04C02


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title: 7817179133_R06C02


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title: 7825611080_R01C01


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title: 7817136114_R05C01


title: 7825611080_R01C02


title: 7817136114_R06C02


title: 7817136114_R04C01


title: 7817136114_R02C01


title: 7817136114_R04C02


title: 7817136114_R02C02


title: 7825611080_R02C02


title: 7811252050_R05C01


title: 7817136114_R01C02


title: 7817161037_R05C02


title: 7817136114_R01C01


title: 7817161037_R06C01


title: 7817161037_R05C01


title: 7817161037_R04C02


title: 7825611080_R03C01


title: 7817161037_R03C02


title: 7817161037_R04C01


title: 7817161037_R02C02


title: 7817161037_R01C01


title: 7817161037_R01C02


title: 7817136114_R06C01


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