title: 10X36_2_GCGGACTGACCGAT


title: 10X36_2_GCCGGAACGTGCTA

status: Public on May 11 2018

type: SRA

city: Stockholm

first: Sten

last: Linnarsson

address: Scheeles väg 1

department: Medical Biochemistry and Biophysics

country: Sweden

institute: Karolinska Institutet

library_source: transcriptomic

data_processing:
Demultiplexed  samples were aligned to the reference genome and converted to mRNA molecule counts using  the 10X Chromium “cellranger” pipeline version 1.1
Genome_build: UCSC mm10
Supplementary_files_format_and_content: Tab-delimited file of mRNA molecule counts

molecule: total RNA

organism: Mus musculus

source_name: Cortex CA1

growth_protocol: Slc32a1  (vesicular  GABA  transporter)‐Cre  BAC  transgenic  mice  were   crossed with a R26R‐tdTomato reporter line to generate mice with fluorescently labelled inhibitory   neurons. Both the Slc32a1‐Cre and tdTomato mouse lines were of mixed B6 and CD1 backgrounds.   Three of these mice were used for both the p28 and p63 cohorts; both males and females were   used at each age.

extract_protocol:
Mice were sacrificed by an overdose of Isoflurane and Ketamine/Xylazine, followed by   transcardial  perfusion  through  the  left  ventricle  with  artificial  cerebrospinal  fluid  (aCSF)   equilibrated in 95%O2 5%CO2 before use. The brain was removed and CA1 was microdissected   from  300μm  vibratome  sections.  Single‐cell  suspensions  were  prepared  using  Papain   (Worthington)  with  30min  enzymatic  digestion,  followed  by  manual  trituration  with  fire‐  polished Pasteur pipettes. The albumin density gradient was only performed for the p63 samples.   On  a  BD  FACSAria  II,  tdTomato‐positive  cells  were  sorted  into  oxygenated  aCSF  at  4°C,   concentrated, inspected for viability, and counted.
Sorted  suspensions  were  added  to  10X  Chromium  RT  mix  aiming  at  2500  cells  recovered  per   experiment. Downstream cDNA synthesis (14 PCR cycles) and library preparation were carried   out  as  instructed  by  the  manufacturer  (10X  Genomics  Chromium  Single  Cell  Kit  Version  1).   Libraries were sequenced on the Illumina HiSeq2500 to an average depth 112 000 reads per cell   (raw),  yielding  on  average  3600  distinct  molecules  and  1700  genes  per  cell.

tag: developmental stage

tag: cell type

value: CA1 inhibitory cells

tag: cell class

value: Unknown


title: 10X36_2_GCCGAGTGACCGAT


title: 10X36_2_GCGCACGAAAGGGC


title: 10X36_2_GCCGGAACTTACTC


title: 10X36_2_GCCCATACTAAGGA


title: 10X36_2_GCCGAGTGTTCTCA


title: 10X36_2_GCCATCACGGAAAT


title: 10X36_2_GCCACTACCTGACA


title: 10X36_2_GCCATCACACACGT


title: 10X36_2_GCCACTACAGAGAT


title: 10X36_2_GCATGATGAAGTAG


title: 10X36_2_GCATGATGATGTCG


title: 10X36_2_GCAGTTGATGTGAC


title: 10X36_2_GCAGGCACGTCAAC


title: 10X36_2_GCAGGCACCACTAG


title: 10X36_2_GCAGCGTGAGTGTC


title: 10X36_2_GCAGCGTGAGATGA


title: 10X36_2_GCAGATACTTCCGC


title: 10X36_2_GCACTAGACCGTAA


title: 10X36_2_GCACCTTGATGTGC


title: 10X36_2_GCACCTTGATTTCC


title: 10X36_2_GCACCACTGCTGAT


title: 10X36_2_GCACCACTACGCAT


title: 10X36_2_GATTTGCTAAGGGC


title: 10X36_2_GATTTAGACAGTTG


title: 10X36_2_GCACAAACCTCAGA


title: 10X36_2_GCAACCCTTGAACC


title: 10X36_2_GATTGGTGTAGCCA


title: 10X36_2_GATGCAACGGAAGC


title: 10X36_2_GATGCCCTGGAGTG


title: 10X36_2_GATTCGGACCTATT


title: 10X36_2_GATTCGGACATGGT


title: 10X36_2_GCCAAAACTTATCC


title: 10X36_2_GATTACCTAAGAAC


title: 10X36_2_GATCTTTGGACGTT


title: 10X36_2_GATCTTACCTACCC


title: 10X36_2_GATCGAACAGTCTG


title: 10X36_2_GATGACACCAATCG


title: 10X36_2_GATCTTTGGGAACG


title: 10X36_2_GATCCGCTAGTACC


title: 10X36_2_GATCCCTGTGAACC


title: 10X36_2_GATCCCTGTCTAGG


title: 10X36_2_GATCCCTGTCACCC


title: 10X36_2_GATCCCTGAGAGTA


title: 10X36_2_GCAGATACGGTATC


title: 10X36_2_GATTGGACCAGTCA


title: 10X36_2_GATTCGGATAACCG


title: 10X36_2_GATCCCTGAACGTC


title: 10X36_2_GATATAACATTCTC


title: 10X36_2_GATAGAGACTTAGG


title: 10X36_2_GATATAACAGATGA


title: 10X36_2_GATACTCTGAACTC


title: 10X36_2_GAGTCAACGTACCA


title: 10X36_2_GAGGTTTGACCTGA


title: 10X36_2_GAGGGTGACAGAAA


title: 10X36_2_GAGTGACTAAAGCA


title: 10X36_2_GAGTACTGCTACGA


title: 10X36_2_GATACTCTAAGATG


title: 10X36_2_GAGTTGTGACTAGC


title: 10X36_2_GAGGGATGCTTCGC


title: 10X36_2_GAGGGAACAGTGCT


title: 10X36_2_GAGGCCACTTTACC


title: 10X36_2_GAGCGCTGTCTTCA


title: 10X36_2_GAGCGGCTGTGAGG


title: 10X36_2_GAGGACGAAGATCC


title: 10X36_2_GAGGGATGACCACA


title: 10X36_2_GATATAACGTCTTT


title: 10X36_2_GAGCATACAGGAGC


title: 10X36_2_GAGAAATGTGCTAG


title: 10X36_2_GAGAGGTGGCAGAG


title: 10X36_2_GAGAAATGAACGTC


title: 10X36_2_GAGATCACCCTCCA


title: 10X36_2_GACTTTACTCTGGA


title: 10X36_2_GACTTTACTCTTCA


title: 10X36_2_GACTCCTGGGAACG


title: 10X36_2_GACTGAACTCTACT


title: 10X36_2_GAGGTACTTCGCTC


title: 10X36_2_GACGTCCTGCGATT


title: 10X36_2_GAGCGAGAAGTCAC


title: 10X36_2_GACGTAACGGTCTA


title: 10X36_2_GACCAAACCTCATT


title: 10X36_2_GACCAAACCCCTAC


title: 10X36_2_GACCTCTGCTGCTC


title: 10X36_2_GACCAAACTGACTG


title: 10X36_2_GACCCTACAAACGA


title: 10X36_2_GACATTCTTTTGTC


title: 10X36_2_GACCTAGAGCTGTA


title: 10X36_2_GACAGTACGTAAGA


title: 10X36_2_GACACTGAGCGGAA


title: 10X36_2_GACGATTGCCTTCG


title: 10X36_2_GAATTAACATACCG


title: 10X36_2_GACTGAACTGTTTC


title: 10X36_2_GAATGGCTTTGTCT


title: 10X36_2_GAATGGCTCATGGT


title: 10X36_2_GAATGCACCCTACC


title: 10X36_2_GAAGTAGAGACGAG


title: 10X36_2_GACAGTTGGAACCT


title: 10X36_2_GAATTAACTATTCC


title: 10X36_2_GAAGGGTGTTCTCA


title: 10X36_2_GAAGCTTGTCTCTA


title: 10X36_2_GAAGGTCTAACGAA


title: 10X36_2_GACGCTCTCTCAAG


title: 10X36_2_GACGGCACGGTACT


title: 10X36_2_GAAGCTTGGGACAG


title: 10X36_2_GAATGCTGAGCATC


title: 10X36_2_GACTGTGAAAGGTA


title: 10X36_2_GAAGCGGATGACCA


title: 10X36_2_GAAGCGGAGTGAGG


title: 10X36_2_GAACGTTGGAACTC


title: 10X36_2_GACAGTTGTGCAAC


title: 10X36_2_GAACCTGATTGGCA


title: 10X36_2_GAAGATGATTCATC


title: 10X36_2_GAAGATGATTGAGC


title: 10X36_2_GAAGATGAGCCTTC


title: 10X36_2_GAAATACTCGGGAA


title: 10X36_2_GAAAGCCTTAGCGT


title: 10X36_2_GAACACACCGAATC


title: 10X36_2_GAAAGATGCCTATT


title: 10X36_2_CTTTAGTGACTCTT


title: 10X36_2_GAAACCTGTGGTGT


title: 10X36_2_CTTTCAGAAGACTC


title: 10X36_2_CTTTGATGTGACTG


title: 10X36_2_GAAACCTGGCTTCC


title: 10X36_2_GAAACAGACTCGAA


title: 10X36_2_CTTGTATGTCTCAT


title: 10X36_2_CTTGAACTTACTGG


title: 10X36_2_CTTGAGGAGGATTC


title: 10X36_2_GAAGCTTGCTTATC


title: 10X36_2_CTTCTAGACGTAGT


title: 10X36_2_GAAGATGACGACAT


title: 10X36_2_CTTACTGAGGGACA


title: 10X36_2_CTTACTGATTCGTT


title: 10X36_2_CTGTGAGACGTACA


title: 10X36_2_GAACCTGAGCGAGA


title: 10X36_2_CTTAAAGAGCTGAT


title: 10X36_2_CTGTATACGAATGA


title: 10X36_2_CTTAAGCTCTGAAC


title: 10X36_2_GAAATACTGACGGA


title: 10X36_2_CTGAGCCTCGTGTA


title: 10X36_2_CTGCCAACCCGCTT


title: 10X36_2_CTTTAGTGAGCCAT


title: 10X36_2_CTGAGAACGACTAC


title: 10X36_2_CTGAATCTAAAAGC


title: 10X36_2_CTGAGCCTATGTCG


title: 10X36_2_CTGAAGACGAGATA


title: 10X36_2_CTGAAGTGACTCAG


title: 10X36_2_CTGAAGACCTTCTA


title: 10X36_2_CTCTAATGATCGTG


title: 10X36_2_CTCGCATGGACTAC


title: 10X36_2_CTGAACGATTCACT


title: 10X36_2_CTCGAGCTCGACAT


title: 10X36_2_CTGAACGAAACGGG


title: 10X36_2_CTCGCATGAGAAGT


title: 10X36_2_CTCGACACTGCTAG


title: 10X36_2_CTCTAATGTGCGTA


title: 10X36_2_CTCGACACTGAGCT


title: 10X36_2_CTCGACACATCGTG


title: 10X36_2_CTCCGAACGAACCT


title: 10X36_2_CTCGACACAAAAGC


title: 10X36_2_CTCAGCTGGGAGCA


title: 10X36_2_CTGAGCCTTCTCCG


title: 10X36_2_CTCAGAGAAAGATG


title: 10X36_2_CTCAGGCTCCTACC


title: 10X36_2_CTCAATTGCTGTGA


title: 10X36_2_CTCAATTGACTACG


title: 10X36_2_CTATGACTCTTATC


title: 10X36_2_CTATCATGTAGAAG


title: 10X36_2_CTCTAATGTGGAAA


title: 10X36_2_CTATACTGAGCATC


title: 10X36_2_CTATACTGGCGATT


title: 10X36_2_CTATAGCTATACCG


title: 10X36_2_CTAGTTTGTGGTCA


title: 10X36_2_CTAGGTGAACCTGA


title: 10X36_2_CTAGTTTGCTTACT


title: 10X36_2_CTACGGCTGCTAAC


title: 10X36_2_CTACTCCTGTCTTT


title: 10X36_2_CTAGAGACGCGTAT


title: 10X36_2_CTAGGATGTCAGTG


title: 10X36_2_CTACGGCTAGCCTA


title: 10X36_2_CTAGAGACGCGAAG


title: 10X36_2_CTCATTGAGGATCT


title: 10X36_2_CTAATAGAAAGCAA


title: 10X36_2_CTAATAGAGCCATA


title: 10X36_2_CTAATAGAAACTGC


title: 10X36_2_CTACGCACTCAAGC


title: 10X36_2_CGTTAGGAGTCGTA


title: 10X36_2_CGTTAGGACGTAGT


title: 10X36_2_CGTGTAGAAGACTC


title: 10X36_2_CGTTAACTCTTAGG


title: 10X36_2_CGTGATGATGTTCT


title: 10X36_2_CGTGATGAGGACTT


title: 10X36_2_CTAGGTGAGTTCTT


title: 10X36_2_CGTGAAACGATGAA


title: 10X36_2_CGTCCATGTTTACC


title: 10X36_2_CTAGATCTAAGAAC


title: 10X36_2_CGTCGACTACACAC


title: 10X36_2_CGTCCAACGTCTAG


title: 10X36_2_CGTCCAACGAACTC


title: 10X36_2_CGTCAAGAGGCGAA


title: 10X36_2_CGTCCAACAGAGGC


title: 10X36_2_CGTCAAGACGAGTT


title: 10X36_2_CGTAACGATCCTTA


title: 10X36_2_CGTACCTGACCCTC


title: 10X36_2_CGGGCATGCCAACA


title: 10X36_2_CGGGACTGTCCTGC


title: 10X36_2_CGGGACTGTTTGCT


title: 10X36_2_CGTACAGACTACTT


title: 10X36_2_CGTACCTGTTTGTC


title: 10X36_2_CTAAGGACTTTGTC


title: 10X36_2_CGTGTAGAATTTCC


title: 10X36_2_CGGCGAACTGCTGA


title: 10X36_2_CGGCGATGTTTACC


title: 10X36_2_CTAAGGACGTAGCT


title: 10X36_2_CGGATAACTCCGTC


title: 10X36_2_CGGATAACGCAAGG


title: 10X36_2_CGGATATGTGATGC


title: 10X36_2_CGGATAACACCCTC


title: 10X36_2_CGGACTCTCTAGAC


title: 10X36_2_CGGAGGCTCGTTAG


title: 10X36_2_CGGAGGCTACGCTA


title: 10X36_2_CGGAATTGCATGCA


title: 10X36_2_CGCTCATGGGTACT


title: 10X36_2_CGGACCGACCTGAA


title: 10X36_2_CGCTACTGAGCATC


title: 10X36_2_CGCTACTGGCGAGA


title: 10X36_2_CGCTAAGACCACCT


title: 10X36_2_CGCTAAGACGAGAG


title: 10X36_2_CGCGATCTAGTCAC


title: 10X36_2_CGCCTAACATTTCC


title: 10X36_2_CGCGAGACGTCACA


title: 10X36_2_CGCACTTGTTCGTT


title: 10X36_2_CGCAGGACGCGATT


title: 10X36_2_CGCCATACTCTCCG


title: 10X36_2_CGCACTTGGAGCAG


title: 10X36_2_CGCACGGACTTCTA


title: 10X36_2_CGATCCACCTGTAG


title: 10X36_2_CGCCATACAGCTCA


title: 10X36_2_CGCACTACCCATGA


title: 10X36_2_CGATAGACTGAGCT


title: 10X36_2_CGATAGACGTGCAT


title: 10X36_2_CGATCAGATGAGCT


title: 10X36_2_CGATCAGACTAAGC


title: 10X36_2_CGAGGGCTAAGGTA


title: 10X36_2_CGAGCGTGGAAAGT


title: 10X36_2_CGAGCCGAAGATGA


title: 10X36_2_CGAGATTGACCATG


title: 10X36_2_CGAGCCGAGTACCA


title: 10X36_2_CGACTGCTCTTACT


title: 10X36_2_CGACGTCTACCTAG


title: 10X36_2_CGACGTCTCGGAGA


title: 10X36_2_CGACCTTGGGGACA


title: 10X36_2_CGCGGATGGAACTC


title: 10X36_2_CGACAAACGACGTT


title: 10X36_2_CGACCACTACCACA


title: 10X36_2_CGACCGGAGGCGAA


title: 10X36_2_CGAATCGAGGAAAT


title: 10X36_2_CGAAGTACTACTTC


title: 10X36_2_CGAAGGGACTCATT


title: 10X36_2_CGAAGACTAAGATG


title: 10X36_2_CGAACATGTGCCCT


title: 10X36_2_CGAACATGCCACAA


title: 10X36_2_CGAAGACTACTTTC


title: 10X36_2_CCTTCACTGAGGCA


title: 10X36_2_CCTTCACTTCATTC


title: 10X36_2_CCTTCACTCGAATC


title: 10X36_2_CGAATCGATAACGC


title: 10X36_2_CCTGCAACCTCATT


title: 10X36_2_CGACTGCTGTATGC


title: 10X36_2_CCTGAGCTTGGTGT


title: 10X36_2_CGACTCACGTTTGG


title: 10X36_2_CCTTAATGACCTTT


title: 10X36_2_CCTCGAACTGGAAA


title: 10X36_2_CCTGCAACAAGAGT


title: 10X36_2_CCTCATCTTCTCGC


title: 10X36_2_CCTCGAACTTGGCA


title: 10X36_2_CCTATTGACCAGTA


title: 10X36_2_CCTATTGAAAGGCG


title: 10X36_2_CCTATAACATCACG


title: 10X36_2_CCTAGAGAGTTGAC


title: 10X36_2_CCTACCGAACCAGT


title: 10X36_2_CCTAAGGATTACTC


title: 10X36_2_CCTAAACTTGTAGC


title: 10X36_2_CCTAAACTAACAGA


title: 10X36_2_CCGTACACTAACCG


title: 10X36_2_CCGGAGACGTACCA


title: 10X36_2_CCGCTATGTTGTCT


title: 10X36_2_CCGGAGACGGGATG


title: 10X36_2_CCGCTATGGTATCG


title: 10X36_2_CCGATAGACCACCT


title: 10X36_2_CCTGCAACCCAAGT


title: 10X36_2_CCGACTACTTGCGA


title: 10X36_2_CCCTTACTGTTCTT


title: 10X36_2_CCCTTACTACCATG


title: 10X36_2_CCCTACGAATCTTC


title: 10X36_2_CCCTGAACTCCTAT


title: 10X36_2_CCCTAGTGTTTGGG


title: 10X36_2_CCCAGTTGGACTAC


title: 10X36_2_CCCGAACTTCGACA


title: 10X36_2_CCCGGAGACTTAGG


title: 10X36_2_CCCACATGTACTGG


title: 10X36_2_CCCAACTGAACCAC


title: 10X36_2_CCCAAAGATGCTAG


title: 10X36_2_CCATGCTGTCTAGG


title: 10X36_2_CCCAAAGAGAAACA


title: 10X36_2_CCATCGTGTAGACC


title: 10X36_2_CCATCGTGGGTTTG


title: 10X36_2_CCATCGTGATTCTC


title: 10X36_2_CCATCCGAGTAGCT


title: 10X36_2_CCATCCGAAAAGCA


title: 10X36_2_CCATATACAGCAAA


title: 10X36_2_CCAGTGCTCAGATC


title: 10X36_2_CCAGCGGATGACCA


title: 10X36_2_CCATAGGAGTCGTA


title: 10X36_2_CCGACACTTCAGTG


title: 10X36_2_CCAGACCTAGCGTT


title: 10X36_2_CCAGACCTACGGAG


title: 10X36_2_CCCTCAGATGTGGT


title: 10X36_2_CCACCTGAAGTCGT


title: 10X36_2_CCCAACACGACACT


title: 10X36_2_CCAGATGATATGCG


title: 10X36_2_CCACTTCTGGTCTA


title: 10X36_2_CCAGATGAGCGAGA


title: 10X36_2_CCAAGAACGTTTGG


title: 10X36_2_CCAAGAACCACAAC


title: 10X36_2_CCAAGATGACACCA


title: 10X36_2_CCAAGTGAAGTGCT


title: 10X36_2_CCAATGGAACTTTC


title: 10X36_2_CCAACCTGAAAACG


title: 10X36_2_CCACCATGTAGCCA


title: 10X36_2_CCAAAGTGTATCTC


title: 10X36_2_CCAAGTGAGGTTAC


title: 10X36_2_CATTTGACTATGGC


title: 10X36_2_CATTTCGATCAAGC


title: 10X36_2_CATTTCGAAAGGCG


title: 10X36_2_CATTAGCTGTTGAC


title: 10X36_2_CATTGACTCTCTAT


title: 10X36_2_CATTAGCTGTACGT


title: 10X36_2_CATGCGCTGTCTGA


title: 10X36_2_CATCGCTGGAGGAC


title: 10X36_2_CATCCCGATCTATC


title: 10X36_2_CATGCGCTCTCTCG


title: 10X36_2_CATGTTACTTACCT


title: 10X36_2_CATCTTGACTTTAC


title: 10X36_2_CATACTTGACCATG


title: 10X36_2_CATCATACGCCATA


title: 10X36_2_CATAACCTCACCAA


title: 10X36_2_CATACTACGTTACG


title: 10X36_2_CATACTACCTTAGG


title: 10X36_2_CAGTTGGATTTGCT


title: 10X36_2_CAGTTGGATGCAGT


title: 10X36_2_CAGTTTACATGTGC


title: 10X36_2_CAGTTGGAGGAAGC


title: 10X36_2_CAGTCAGAATACCG


title: 10X36_2_CAGTGATGGAAAGT


title: 10X36_2_CAGGGCACGCATAC


title: 10X36_2_CAGGTAACTTCATC


title: 10X36_2_CAGCTCACGCTATG


title: 10X36_2_CAGCTAGACATCAG


title: 10X36_2_CATTACACATGTGC


title: 10X36_2_CATGGCCTGTTGCA


title: 10X36_2_CAGCGGACTGCCAA


title: 10X36_2_CAGCACCTCATTGG


title: 10X36_2_CAGCATGACTTCGC


title: 10X36_2_CATCGCTGATGACC


title: 10X36_2_CAGATGACTGTCCC


title: 10X36_2_CAGACTGATATTCC


title: 10X36_2_CAGACTGATTGCTT


title: 10X36_2_CACTTTGATCTAGG


title: 10X36_2_CACTTTGAACGGGA


title: 10X36_2_CACTGCACGTGCTA


title: 10X36_2_CAGATCGAAGATGA


title: 10X36_2_CAGATCGAAGTCAC


title: 10X36_2_CACTGCTGACTGTG


title: 10X36_2_CAGGTTGATTCTGT


title: 10X36_2_CACTAACTCTCAGA


title: 10X36_2_CACGGGTGACTAGC


title: 10X36_2_CACTCCGACGACAT


title: 10X36_2_CACTGAGACAACCA


title: 10X36_2_CAGCATGATACGCA


title: 10X36_2_CACTGAGACCTACC


title: 10X36_2_CACGGGACTTCCAT


title: 10X36_2_CACGGGACCTCTCG


title: 10X36_2_CACGGGACTCTACT


title: 10X36_2_CACCGGGAGTGTCA


title: 10X36_2_CACCCATGATGCCA


title: 10X36_2_CACTCCGAGAGCAG


title: 10X36_2_CACCGGGATTTGGG


title: 10X36_2_CACCACTGAGTGTC


title: 10X36_2_CACATGGATCTCAT


title: 10X36_2_CACATGGATACTGG


title: 10X36_2_CACATACTCATACG


title: 10X36_2_CACATACTAACGGG


title: 10X36_2_CACAGCCTGTGTTG


title: 10X36_2_CACAGATGCTGGAT


title: 10X36_2_CACAGAACTACTTC


title: 10X36_2_CACAGATGCTAGTG


title: 10X36_2_CACAGATGGAGCTT


title: 10X36_2_CACGAAACGTATGC


title: 10X36_2_CACAGCCTCGCTAA


title: 10X36_2_CACACCTGTACGCA


title: 10X36_2_CAATCTACTGTTTC


title: 10X36_2_CACCGTTGGAATCC


title: 10X36_2_CAAGTCGACTTGAG


title: 10X36_2_CAATCGGAATCTCT


title: 10X36_2_CAATAATGAGTACC


title: 10X36_2_CAAGCTGAACGTGT


title: 10X36_2_CAAGGACTACGCTA


title: 10X36_2_CAAGGTTGCAGTCA


title: 10X36_2_CAAGCCCTTAGAGA


title: 10X36_2_CAAGCATGGGACAG


title: 10X36_2_CAACGAACCTGCAA


title: 10X36_2_CAACCGCTGCATAC


title: 10X36_2_CAAAGCACACACTG


title: 10X36_2_CACATACTACACTG


title: 10X36_2_CAAAGCTGTCTGGA


title: 10X36_2_CAAATATGGGTTCA


title: 10X36_2_ATTTCTCTCCGTAA


title: 10X36_2_ATTTGCACATTCTC


title: 10X36_2_CACAGTGAAGAGAT


title: 10X36_2_ATTGGTCTCAGTTG


title: 10X36_2_ATTTCGTGGAGATA


title: 10X36_2_ATTGCTACGACAGG


title: 10X36_2_ATTGCTTGGTTCAG


title: 10X36_2_ATTGGGTGCAGTCA


title: 10X36_2_CAATAATGGTACAC


title: 10X36_2_ATTCCATGTGCTTT


title: 10X36_2_ATTCGGGAGGGTGA


title: 10X36_2_ATTGCGGAGTCAAC


title: 10X36_2_ATTCTTCTGCGAAG


title: 10X36_2_ATTAGATGTAGTCG


title: 10X36_2_ATTCGGGATCGTAG


title: 10X36_2_ATTAGATGGACTAC


title: 10X36_2_ATTCGACTCAATCG


title: 10X36_2_CAAGACACACCTAG


title: 10X36_2_ATTACCACAGAGAT


title: 10X36_2_CAAAGCACGACAGG


title: 10X36_2_ATTACCTGGAATAG


title: 10X36_2_ATTAACGATGACCA


title: 10X36_2_ATTAAGACAAGAGT


title: 10X36_2_ATTAACGACGAATC


title: 10X36_2_ATTAACGAACTCTT


title: 10X36_2_ATGTCGGACGTCTC


title: 10X36_2_ATTGAAACTCGTGA


title: 10X36_2_ATGTCACTTCTCGC


title: 10X36_2_ATGTACCTGTAGGG


title: 10X36_2_ATTCGACTACCAAC


title: 10X36_2_ATTAGTGAGTGTAC


title: 10X36_2_ATTACCACAGCTCA


title: 10X36_2_ATGCAGTGGTTTGG


title: 10X36_2_ATGCAGTGGAATCC


title: 10X36_2_ATGCCGCTAGCTCA


title: 10X36_2_ATGCGATGGAAGGC


title: 10X36_2_ATGGGTACCATGAC


title: 10X36_2_ATGTACCTCAGGAG


title: 10X36_2_ATGCAGACGGTAGG


title: 10X36_2_ATGGTGACTGTGGT


title: 10X36_2_ATGTAAACGGGTGA


title: 10X36_2_ATGAAACTTAACGC


title: 10X36_2_ATCTTTCTTAAAGG


title: 10X36_2_ATCTTTCTACGCAT


title: 10X36_2_ATCTGTTGTAGAAG


title: 10X36_2_ATCTGGGAAATGCC


title: 10X36_2_ATCTGACTCCCTAC


title: 10X36_2_ATCTACTGTGGAAA


title: 10X36_2_ATCTACTGTCCAGA


title: 10X36_2_ATCTACTGTTGGTG


title: 10X36_2_ATCGTTTGAATGCC


title: 10X36_2_ATCTACACGGAAAT


title: 10X36_2_ATCGCAGACAGTCA


title: 10X36_2_ATCGCGCTTGGAGG


title: 10X36_2_ATCGTTTGCCAATG


title: 10X36_2_ATCTCAACTTGACG


title: 10X36_2_ATGCCAGATTCACT


title: 10X36_2_ATCGCAGAAGCCTA


title: 10X36_2_ATCATCTGGCAAGG


title: 10X36_2_ATCCAGGACCTTTA


title: 10X36_2_ATCACTTGCCCTAC


title: 10X36_2_ATCACTACTAGACC


title: 10X36_2_ATCCCGTGGCAGAG


title: 10X36_2_ATCCATACCGTTGA


title: 10X36_2_CACGCTACTGCGTA


title: 10X36_2_ATCACTTGCTTGCC


title: 10X36_2_ATCGACGAAGTCTG


title: 10X36_2_ATCCAGGAGATGAA


title: 10X36_2_ATCTGGGAGTAGCT


title: 10X36_2_ATCACTTGCAAGCT


title: 10X36_2_ATATAGTGACCCTC


title: 10X36_2_ATATGCCTCGTTGA


title: 10X36_2_ATCAAATGGATAAG


title: 10X36_2_ATAGGCTGAGTACC


title: 10X36_2_ATATGAACGAGGCA


title: 10X36_2_ATAGGAGACACTAG


title: 10X36_2_ATACGGACCCTTCG


title: 10X36_2_ATACGTCTGAAACA


title: 10X36_2_ATACGTCTAAGGTA


title: 10X36_2_ATACCACTTCCTGC


title: 10X36_2_ATAGAACTATCGTG


title: 10X36_2_ATACCGGATGACAC


title: 10X36_2_ATACCACTATTCGG


title: 10X36_2_ATACAATGCCTTTA


title: 10X36_2_ATAATCGATCAGAC


title: 10X36_2_ATCACACTCTACGA


title: 10X36_2_AGTTGTCTAACGGG


title: 10X36_2_ATAACCCTCGCAAT


title: 10X36_2_AGTTTCACTGAAGA


title: 10X36_2_AGTTTCACTGTTCT


title: 10X36_2_AGTTTGCTTCCTAT


title: 10X36_2_ATAACAACGGTTTG


title: 10X36_2_AGTTCTACTGTCTT


title: 10X36_2_AGTTCTACTTTCTG


title: 10X36_2_AGTTATGACTGACA


title: 10X36_2_AGTTAAACAGGTCT


title: 10X36_2_ATCGACGACTGTCC


title: 10X36_2_AGTGTTCTATCGAC


title: 10X36_2_AGTGCAACCTGATG


title: 10X36_2_AGTGACTGCCCTTG


title: 10X36_2_AGTGACTGTTCGCC


title: 10X36_2_AGTGAAGATCCTGC


title: 10X36_2_AGTATAACGCTGAT


title: 10X36_2_AGTATAACAGAACA


title: 10X36_2_AGTACGTGGAAAGT


title: 10X36_2_AGGTTGTGCCTACC


title: 10X36_2_AGTCAGACGTAGGG


title: 10X36_2_AGGTTCGATGCCTC


title: 10X36_2_AGTCACGAATCGGT


title: 10X36_2_ATACCTACACTGTG


title: 10X36_2_ATAAACACAAGAGT


title: 10X36_2_AGGTTCGACAGAAA


title: 10X36_2_AGGTGTTGAGCAAA


title: 10X36_2_AGGTCTGAGTCATG


title: 10X36_2_AGGGCCTGCGTAAC


title: 10X36_2_AGGGTTTGGCTTCC


title: 10X36_2_AGGGTGGAGACGAG


title: 10X36_2_AGGTCTGAATTCTC


title: 10X36_2_AGGGAGTGTGTTCT


title: 10X36_2_AGGGTGGAGTGAGG


title: 10X36_2_AGGCCTCTATCACG


title: 10X36_2_AGGCTAACTCCTCG


title: 10X36_2_AGTTAAACTAACGC


title: 10X36_2_AGGGTTTGCAGTCA


title: 10X36_2_AGGGTGGATTACCT


title: 10X36_2_AGGGAGTGGTATGC


title: 10X36_2_AGGGCGCTCCATGA


title: 10X36_2_AGGCCTCTGTTGTG


title: 10X36_2_AGGAGTCTCCACCT


title: 10X36_2_AGGATGCTTCTTAC


title: 10X36_2_AGCTTACTCGTACA


title: 10X36_2_AGCTTACTCCCTAC


title: 10X36_2_AGCTGTGATAACCG


title: 10X36_2_AGCTTACTCAGATC


title: 10X36_2_AGGCAACTCTACGA


title: 10X36_2_AGCTGTGACTTCGC


title: 10X36_2_AGCTGCCTTAGACC


title: 10X36_2_AGCTGCCTGGCAAG


title: 10X36_2_AGCTGCCTCGAGAG


title: 10X36_2_AGCTGCCTCCTATT


title: 10X36_2_AGCCTCACCTCCCA


title: 10X36_2_AGCGGGCTCGCAAT


title: 10X36_2_AGCGAACTCATGGT


title: 10X36_2_AGGCAGGAACCGAT


title: 10X36_2_AGCCTCACGAAAGT


title: 10X36_2_AGCCGTCTCGCATA


title: 10X36_2_AGCAACACGTACGT


title: 10X36_2_AGCAACACGTTTGG


title: 10X36_2_AGCATGACCAGAGG


title: 10X36_2_AGCGGCACCATGGT


title: 10X36_2_AGCTGAACCGGTAT


title: 10X36_2_AGATATTGTAAGGA


title: 10X36_2_AGATTAACACTGGT


title: 10X36_2_AGCGAACTGGTTCA


title: 10X36_2_AGCCGTCTAACTGC


title: 10X36_2_AGCAAAGACACACA


title: 10X36_2_AGCGGCACTTTGCT


title: 10X36_2_AGATATACCAACTG


title: 10X36_2_AGCCACCTAGTGTC


title: 10X36_2_AGATATACAGTCGT


title: 10X36_2_AGCATGACACGGAG


title: 10X36_2_AGCAAAGACCACAA


title: 10X36_2_AGGGCGCTCTCCAC


title: 10X36_2_AGATATACCCTTCG


title: 10X36_2_AGCCGGTGTGTCCC


title: 10X36_2_AGCATGACTGGTAC


title: 10X36_2_AGCCGGACTTCCCG


title: 10X36_2_AGAGTGCTTTTCTG


title: 10X36_2_AGATCTCTACCCAA


title: 10X36_2_AGAGAAACGCGAGA


title: 10X36_2_AGAGAAACACCCTC


title: 10X36_2_AGAGAAACCTAGTG


title: 10X36_2_AGACCTGAGGTGAG


title: 10X36_2_AGACTCGAGCTCCT


title: 10X36_2_AGAATTTGTTCTTG


title: 10X36_2_AGACTCGATGACTG


title: 10X36_2_AGAATACTAAGGGC


title: 10X36_2_AGACACTGGTACGT


title: 10X36_2_AGAATGGAAGAAGT


title: 10X36_2_AGAATACTTCGATG


title: 10X36_2_AGAATACTGAGAGC


title: 10X36_2_ACTTGTACTTGACG


title: 10X36_2_ACTTTGTGTTGCGA


title: 10X36_2_ACTTGTACAACCTG


title: 10X36_2_AGAACGCTTAACGC


title: 10X36_2_AGAAACGACTAGCA


title: 10X36_2_AGAAGATGAGAGGC


title: 10X36_2_AGAATGGAGACGGA


title: 10X36_2_ACTTGACTACACTG


title: 10X36_2_ACTTCAACTCCGAA


title: 10X36_2_ACTTGACTCGACAT


title: 10X36_2_ACTTGGGACCTTCG


title: 10X36_2_ACTTCAACGCGATT


title: 10X36_2_ACTGCCTGAGGCGA


title: 10X36_2_AGACACTGACACGT


title: 10X36_2_ACTGCCACGTTGAC


title: 10X36_2_ACTCTCCTTTTCTG


title: 10X36_2_ACTGCCACCTTAGG


title: 10X36_2_ACTGCCACGCCCTT


title: 10X36_2_ACTCGCACCACTAG


title: 10X36_2_ACTTAAGAAAGTAG


title: 10X36_2_ACTCGCACCTGAGT


title: 10X36_2_ACTTAAGAAGCTAC


title: 10X36_2_ACTCGAGAACCTGA


title: 10X36_2_ACGTTTACCATACG


title: 10X36_2_ACGTTGGAAGATGA


title: 10X36_2_ACTCCTCTGCGGAA


title: 10X36_2_ACTACGGACGAATC


title: 10X36_2_ACTCCTCTTGCCTC


title: 10X36_2_ACGGTCCTCACACA


title: 10X36_2_ACGGTATGGGTGGA


title: 10X36_2_ACGTGATGTTAGGC


title: 10X36_2_ACGTGCCTAACGGG


title: 10X36_2_ACGTGCCTTGTCGA


title: 10X36_2_ACGGCGTGCACACA


title: 10X36_2_ACGGTATGGAATCC


title: 10X36_2_ACGTCCTGCACTGA


title: 10X36_2_ACGCTGCTCGTAAC


title: 10X36_2_ACGCTCACCAGATC


title: 10X36_2_ACGCGGTGCACCAA


title: 10X36_2_GCACGTCTCTACTT


title: 10X36_2_ACGCCTTGTGGTGT


title: 10X36_2_ACGCCGGATTGTCT


title: 10X36_2_ACGCAATGTGCTTT


title: 10X36_2_ACGCCACTGCGAAG


title: 10X36_2_ACGCACCTGTGCTA


title: 10X36_2_ACGTTGGAGGATTC


title: 10X36_2_ACGATTCTCTGGTA


title: 10X36_2_ACGATCGATTCGCC


title: 10X36_2_ACGAGGGACCTTGC


title: 10X36_2_ACGAAGCTGTATGC


title: 10X36_2_ACGAAGCTAGGAGC


title: 10X36_2_ACGTAGACTCTACT


title: 10X36_2_ACGCAATGCGATAC


title: 10X36_2_ACCTGAGATGATGC


title: 10X36_2_ACCCGTTGCATGCA


title: 10X36_2_ACCGAAACTAGACC


title: 10X36_2_ACCCGTACCGGGAA


title: 10X36_2_ACCTTTGATGACCA


title: 10X36_2_ACCCGTTGCATCAG


title: 10X36_2_ACCCAGCTTGTGGT


title: 10X36_2_ACCGAAACGTTACG


title: 10X36_2_ACCTATTGAAGCAA


title: 10X36_2_ACCCACTGAACTGC


title: 10X36_2_ACCCAGCTGGTAGG


title: 10X36_2_ACCCAAGATCTGGA


title: 10X36_2_ACCATTACGGTATC


title: 10X36_2_ACCCAAGAAACCTG


title: 10X36_2_ACGCACCTCTACTT


title: 10X36_2_ACCACGCTAGAGGC


title: 10X36_2_ACCAACGATTAGGC


title: 10X36_2_ACATGGTGGCAGTT


title: 10X36_2_ACATCACTCTAGTG


title: 10X36_2_ACCTTTGAGTCGTA


title: 10X36_2_ACCAACGATCTCGC


title: 10X36_2_ACAGTCGAAGCTAC


title: 10X36_2_ACAGCAACACGACT


title: 10X36_2_ACACGATGTCTTAC


title: 10X36_2_ACACCCTGCTAGCA


title: 10X36_2_ACAATCCTCCTTCG


title: 10X36_2_ACACAGACGCATCA


title: 10X36_2_AATGCGTGCTTACT


title: 10X36_2_AATGGAGATAGACC


title: 10X36_2_ACAAAGGAGTCGAT


title: 10X36_2_ACAAAGGAGTCACA


title: 10X36_2_AATTACGAGTAGCT


title: 10X36_2_AATTGTGAGAGGGT


title: 10X36_2_ACCCAAGAGTATCG


title: 10X36_2_AATTGATGTCTACT


title: 10X36_2_AATCCGGAAGAACA


title: 10X36_2_AATCTCACGTTAGC


title: 10X36_2_AATGTCCTCAGGAG


title: 10X36_2_AATTCCTGACACAC


title: 10X36_2_AATCGGTGTCCTGC


title: 10X36_2_AATACTGATTCCGC


title: 10X36_2_ACACGAACCCTCGT


title: 10X36_2_AATCCGGAATGCCA


title: 10X36_2_ACCAGTGAAGAACA


title: 10X36_2_AATCAAACGGAGTG


title: 10X36_2_AAGTTCCTGGTTTG


title: 10X36_2_AATAGGGATCCTAT


title: 10X36_2_AAGTCTCTCAGCTA


title: 10X36_2_AATATCGAACGGTT


title: 10X36_2_AATACTGATATGGC


title: 10X36_2_AAGTCCGATGACCA


title: 10X36_2_AAGTAGGACCCTTG


title: 10X36_2_AAGGCTTGGGACTT


title: 10X36_2_AAGCCATGCGCATA


title: 10X36_2_AAGTAGGATAGAAG


title: 10X36_2_AATACTGACTCGCT


title: 10X36_2_AAGCACTGGCTATG


title: 10X36_2_AATAAGCTATCGTG


title: 10X36_2_AATAAGCTAGCACT


title: 10X36_2_AATAAGCTCCAACA


title: 10X36_2_AAGCACTGAGAGTA


title: 10X36_2_AAGATTACGAACCT


title: 10X36_2_AAGTGGCTCCTTTA


title: 10X36_2_AAGAGATGGGTGGA


title: 10X36_2_AAGCACTGCCGTAA


title: 10X36_2_AAGACAGACTAGAC


title: 10X36_2_AAGATTACGGTTTG


title: 10X36_2_AACTGTCTGTGCTA


title: 10X36_2_AAGTAGGATCTCAT


title: 10X36_2_AACTCTTGTGAGCT


title: 10X36_2_AACTACCTTAAGGA


title: 10X36_2_AAGAGATGTGATGC


title: 10X36_2_AAGAGATGACTCAG


title: 10X36_2_AAGTCCGACGAACT


title: 10X36_2_AACGTTCTGAATGA


title: 10X36_2_AACGTGTGTTTCGT


title: 10X36_2_AACCTTTGTGTGCA


title: 10X36_2_AAGACAGACGTCTC


title: 10X36_2_AACGGTTGACCTTT


title: 10X36_2_AACATTGAAGTAGA


title: 10X36_2_AACGGTTGCGAATC


title: 10X36_2_AAGTGCACAAAGTG


title: 10X36_2_AACATTGATTCGGA


title: 10X36_2_AACATATGTCAGGT


title: 10X36_2_AACACGTGCAGATC


title: 10X36_2_AACGCCCTGGTTTG


title: 10X36_2_AACAGAGAGAAGGC


title: 10X36_2_AAATTCGAGACGAG


title: 10X36_2_AAATGGGAGTCGTA


title: 10X36_2_AAATCTGATCGTAG


title: 10X36_2_AACTCACTTTCATC


title: 10X36_2_AAATCAACGTCCTC


title: 10X36_2_AAATCAACTGAGGG


title: 10X36_2_AAATACTGTATTCC


title: 10X36_2_AACCCAGATGGCAT


title: 10X36_2_AAAGCCTGAGAGAT


title: 10X36_2_AAAGAGACGTTACG


title: 10X36_2_AAATACTGGTCTTT


title: 10X36_2_AAACGCACTGCAAC


title: 10X36_2_AAAGACGAGTATCG


title: 10X36_2_AAAGGCCTTGCATG


title: 10X36_1_TTTGCATGTGTGCA


title: 10X36_2_AACAGCACTGTGGT


title: 10X36_2_AAACATTGTGGTGT


title: 10X36_2_AATATCGATGCGTA


title: 10X36_2_AAACTTGAGCGGAA


title: 10X36_1_TTTGACTGATGCCA


title: 10X36_1_TTTCAGTGAACGAA


title: 10X36_1_TTTCCAGAAGGTCT


title: 10X36_1_TTTGCATGCTCTTA


title: 10X36_1_TTTCACGACAGCTA


title: 10X36_1_TTTGCATGGAAACA


title: 10X36_2_AACAAACTTCTACT


title: 10X36_1_TTTAGGCTGGAGTG


title: 10X36_1_TTGTAGCTTTGGTG


title: 10X36_1_TTGTACACCCAATG


title: 10X36_1_TTGGTACTGTCTAG


title: 10X36_2_AAATCATGCCTCCA


title: 10X36_1_TTGCTAACCGAACT


title: 10X36_1_TTCTTACTTTGCTT


title: 10X36_1_TTCTTACTGTACGT


title: 10X36_1_TTCTGATGTGTGAC


title: 10X36_1_TTCTGATGTGCCAA


title: 10X36_1_TTCTCAGATACGAC


title: 10X36_1_TTCTACGAAGCCAT


title: 10X36_1_TTCGATTGCCTTAT


title: 10X36_1_TTCGAGGAACCATG


title: 10X36_1_TTCCCACTAGCGTT


title: 10X36_1_TTCCAAACACTGTG


title: 10X36_1_TTCATTCTTCTTTG


title: 10X36_1_TTCATGTGGCTCCT


title: 10X36_1_TTCCAAACTACGCA


title: 10X36_1_TTCATGTGGCCATA


title: 10X36_1_TTCATTCTAGCACT


title: 10X36_1_TTCATCGAGGAGCA


title: 10X36_1_TTGTACACAGCTCA


title: 10X36_1_TTCACCCTCGACAT


title: 10X36_1_TTCAAGCTTAGACC


title: 10X36_1_TTCAAGCTCAGAAA


title: 10X36_1_TTATGCACCAGGAG


title: 10X36_1_TTATGCACCACTCC


title: 10X36_1_TTAGTCACTGCTCC


title: 10X36_1_TTATGAGATAGTCG


title: 10X36_1_TTAGGGTGGGTAGG


title: 10X36_1_TTAGGTCTCTATTC


title: 10X36_1_TTAGGGTGAAACAG


title: 10X36_1_TTAGGGTGCATTCT


title: 10X36_1_TTCTGATGGCCAAT


title: 10X36_1_TTAGAATGGTCTAG


title: 10X36_1_TTAGAATGCCCACT


title: 10X36_1_TTACTCGATGGTCA


title: 10X36_1_TTACGTACTTGGTG


title: 10X36_1_TTACTCGACTACTT


title: 10X36_1_TTACGTACAGCTCA


title: 10X36_1_TTACAGCTGTCTAG


title: 10X36_1_TTACACACTGCATG


title: 10X36_1_TTACGACTTCAGTG


title: 10X36_1_TTACAGCTCTCCAC


title: 10X36_1_TTACAGCTAGAACA


title: 10X36_1_TTCATCGAAAACGA


title: 10X36_1_TTACGACTTTCTTG


title: 10X36_1_TTAACCACTCCTAT


title: 10X36_1_TGTTACTGGAAAGT


title: 10X36_1_TGTTAAGATGTTCT


title: 10X36_1_TTACACACGTAGCT


title: 10X36_1_TGTGATCTTTGTCT


title: 10X36_1_TGTGATCTGCAGAG


title: 10X36_1_TGTGATCTGACTAC


title: 10X36_1_TGTGAGTGGTGTTG


title: 10X36_1_TGTCTAACTCGTTT


title: 10X36_1_TGTGACGAATCTCT


title: 10X36_1_TGTCTAACCGACTA


title: 10X36_1_TGTATGCTAGCAAA


title: 10X36_1_TGTAGTCTTCTCCG


title: 10X36_1_TGTAGTCTCCTCAC


title: 10X36_1_TGTAGTCTATCGGT


title: 10X36_1_TTCGTATGTCTCGC


title: 10X36_1_TTTATCCTCTGCTC


title: 10X36_1_TGGTTACTACCACA


title: 10X36_1_TGTACTTGGACGTT


title: 10X36_1_TGTAAAACCTGGTA


title: 10X36_1_TGGTCAGATCATTC


title: 10X36_1_TGGTCAGACCAACA


title: 10X36_1_TGGTAGACAGAGAT


title: 10X36_1_TGGTATCTCGGTAT


title: 10X36_1_TGGCAATGCACTTT


title: 10X36_1_TGGATCGATGACCA


title: 10X36_1_TGGAGGGACCAGTA


title: 10X36_1_TGTTAAGACGTTAG


title: 10X36_1_TGGATGACGAGGCA


title: 10X36_1_TGGAGACTTCCTGC


title: 10X36_1_TGGAACACCTGACA


title: 10X36_1_TGTGAGTGACCTAG


title: 10X36_1_TGGAAGCTACTAGC


title: 10X36_1_TGTCTAACCTGTTT


title: 10X36_1_TGCGTAGACGAACT


title: 10X36_1_TGCGATGACTTGAG


title: 10X36_1_TGCGATGACCGAAT


title: 10X36_1_TGCGTAGAAGCACT


title: 10X36_1_TGCGCACTAGCGTT


title: 10X36_1_TGCGAAACAGTTCG


title: 10X36_1_TGCCGACTACGCAT


title: 10X36_1_TGCCAGCTGGAAAT


title: 10X36_1_TGCCAGCTCTTATC


title: 10X36_1_TGCCACTGAGTGCT


title: 10X36_1_TGCCAAGACCCTCA


title: 10X36_1_TGCATGGAGTGCTA


title: 10X36_1_TGCAGATGGAGCTT


title: 10X36_1_TGCAGATGATCGAC


title: 10X36_1_TGCCAAGAGCTTCC


title: 10X36_1_TGCAGATGGACGTT


title: 10X36_1_TGCACAGACGCCTT


title: 10X36_1_TGCTAGGAACACTG


title: 10X36_1_TGATTCACGCTTAG


title: 10X36_1_TGATTAGACAGGAG


title: 10X36_1_TGCAACGAGTTGTG


title: 10X36_1_TGCAAGACTCGCTC


title: 10X36_1_TGATTAGAAGAGTA


title: 10X36_1_TGATTCACAGGCGA


title: 10X36_1_TGCAAGACTTCGCC


title: 10X36_1_TGATCGGAATTCCT


title: 10X36_1_TGATATGAGTCGAT


title: 10X36_1_TGATCACTTTCGTT


title: 10X36_1_TGATATGACGGGAA


title: 10X36_1_TGATACCTAAGATG


title: 10X36_1_TGATAAACCAGAAA


title: 10X36_1_TGAGTGACTTAGGC


title: 10X36_1_TGAGTCGAGCGAAG


title: 10X36_1_TGCGAAACTCGTGA


title: 10X36_1_TGCAGATGCCCGTT


title: 10X36_1_TGAGGTACACAGTC


title: 10X36_1_TGAGCTGAGCTATG


title: 10X36_1_TGAGCTGAGAGGAC


title: 10X36_1_TGCTAGGAGCTGAT


title: 10X36_1_TGACTGGAATCAGC


title: 10X36_1_TGACGATGGGAGTG


title: 10X36_1_TGACGATGATCGGT


title: 10X36_1_TGAGTCGACAATCG


title: 10X36_1_TGACACGACAGAAA


title: 10X36_1_TGACGAACGTAGCT


title: 10X36_1_TGACCAGACGACAT


title: 10X36_1_TGACGATGAGTCGT


title: 10X36_1_TGACCAGAGGTCTA


title: 10X36_1_TGAATAACGTCGTA


title: 10X36_1_TGAATAACCACTTT


title: 10X36_1_TGACCAGACCGATA


title: 10X36_1_TGATACCTCTTCTA


title: 10X36_1_TGAAGCTGAGAATG


title: 10X36_1_TGAAGCACCCTCAC


title: 10X36_1_TCTTCAGACCTTGC


title: 10X36_1_TCTTGATGCACTCC


title: 10X36_1_TGACTTACACCAAC


title: 10X36_1_TCTATGTGTCCTCG


title: 10X36_1_TCTTCAGATCTCGC


title: 10X36_1_TCTATGTGCCGTTC


title: 10X36_1_TCTATGTGCTCGAA


title: 10X36_1_TCTAGACTACGCAT


title: 10X36_1_TCTAACTGGGAGGT


title: 10X36_1_TCTAGACTCTCGAA


title: 10X36_1_TCGTGAGACTTCTA


title: 10X36_1_TCGAGCCTCTGAGT


title: 10X36_1_TCGATTTGTGGATC


title: 10X36_1_TGAAGCACTTGCTT


title: 10X36_1_TCGTGAGATTACTC


title: 10X36_1_TGACACGACTTAGG


title: 10X36_1_TCGACCTGTCAGAC


title: 10X36_1_TCGATACTTGGAAA


title: 10X36_1_TCGTTATGACCACA


title: 10X36_1_TCGTTATGCAGATC


title: 10X36_1_TGAAGCACGAACCT


title: 10X36_1_TCTCAAACGCTATG


title: 10X36_1_TCGGACCTCATTTC


title: 10X36_1_TCGAGAACGAGAGC


title: 10X36_1_TCGATACTCTCTCG


title: 10X36_1_TCCCATCTGGTGTT


title: 10X36_1_TCCCATCTCCTCCA


title: 10X36_1_TCCATCCTCCGCTT


title: 10X36_1_TCATCATGGCAGTT


title: 10X36_1_TCCATAACGTACGT


title: 10X36_1_TCCCACGAGTCCTC


title: 10X36_1_TCATCAACCAGGAG


title: 10X36_1_TCTAGACTGTTGAC


title: 10X36_1_TCAGTTACAAGTAG


title: 10X36_1_TCAGCGCTTGTCAG


title: 10X36_1_TCAGCAGATCAGTG


title: 10X36_1_TCAGGATGGCCAAT


title: 10X36_1_TCGACGCTTCGATG


title: 10X36_1_TCAGCAGACCATAG


title: 10X36_1_TCCCGAACGCTGTA


title: 10X36_1_TCACCTCTTTGACG


title: 10X36_1_TCACCGTGTTATCC


title: 10X36_1_TCACCGTGGGTTTG


title: 10X36_1_TCACCGTGGCGTAT


title: 10X36_1_TCAAGGACCTCTTA


title: 10X36_1_TCACGAGAATGCTG


title: 10X36_1_TCGAGCCTTTTGTC


title: 10X36_1_TCAAGTCTGACGTT


title: 10X36_1_TATGGTCTGTTCAG


title: 10X36_1_TCAAGTCTTGAGAA


title: 10X36_1_TATTGCTGTTTCGT


title: 10X36_1_TCAAGGTGTCCTTA


title: 10X36_1_TCCATAACCTCGCT


title: 10X36_1_TATGGGTGTCAGAC


title: 10X36_1_TATGGGTGCTTCGC


title: 10X36_1_TATGCGGATCCTTA


title: 10X36_1_TATCCAACGAAACA


title: 10X36_1_TATCGTACTCAGTG


title: 10X36_1_TATGAATGTTGGTG


title: 10X36_1_TCACGAGAACCTTT


title: 10X36_1_TCAAGGACGGTAAA


title: 10X36_1_TATCAGCTTGGTGT


title: 10X36_1_TATCAGCTTGTCAG


title: 10X36_1_TATCAGCTTGAGAA


title: 10X36_1_TATCAAGATGCCTC


title: 10X36_1_TATCAGCTCCGATA


title: 10X36_1_TATCCAACAGTCAC


title: 10X36_1_TATCAAGACGTTGA


title: 10X36_1_TATAGATGTCTTTG


title: 10X36_1_TAGTCGGATAACCG


title: 10X36_1_TATAGATGCGAACT


title: 10X36_1_TAGTCACTGACAAA


title: 10X36_1_TAGTAATGACCCAA


title: 10X36_1_TAGGTTCTCGAACT


title: 10X36_1_TAGGTCGAGAATAG


title: 10X36_1_TAGGCAACCTCTTA


title: 10X36_1_TAGGTGACCCAGTA


title: 10X36_1_TAGGCATGCTGAAC


title: 10X36_1_TAGCTACTACACGT


title: 10X36_1_TAGCGATGTGACTG


title: 10X36_1_TAGCCCTGTGATGC


title: 10X36_1_TAGCCCTGGTTTCT


title: 10X36_1_TAGCATCTACCAGT


title: 10X36_1_TAGAGCACTTCGCC


title: 10X36_1_TAGATTGATAGCCA


title: 10X36_1_TAGAGCACAGAGAT


title: 10X36_1_TAGAAACTGTATGC


title: 10X36_1_TAGAATACGGACAG


title: 10X36_1_TACTTGACCTTGAG


title: 10X36_1_TACTTGACCCTACC


title: 10X36_1_TACTGGGACTCTAT


title: 10X36_1_TACTCCCTTGTGAC


title: 10X36_1_TACTCAACTTAGGC


title: 10X36_1_TACTAAGAACCAGT


title: 10X36_1_TACTCTGATAGAAG


title: 10X36_1_TACGGCCTGTTGAC


title: 10X36_1_TAGTATGACTTCCG


title: 10X36_1_TACTCAACCAGATC


title: 10X36_1_TACGTACTGTTGTG


title: 10X36_1_TACGGCCTGTTAGC


title: 10X36_1_TAGGAGCTGACGAG


title: 10X36_1_TACCGCTGTTGCAG


title: 10X36_1_TACGAGTGGATGAA


title: 10X36_1_TACCATTGAGTCTG


title: 10X36_1_TACGGCCTACCTAG


title: 10X36_1_TACGGAACCATTCT


title: 10X36_1_TAATGATGTCTCTA


title: 10X36_1_TACGATCTCATCAG


title: 10X36_1_TACCGCTGTCGTAG


title: 10X36_1_TACATAGAGCTGAT


title: 10X36_1_TACCATTGCGGTAT


title: 10X36_1_TAATCGCTGTTCGA


title: 10X36_1_TAATCCACTGAAGA


title: 10X36_1_TAAGTCCTCCTCGT


title: 10X36_1_TAAGTCCTGTACGT


title: 10X36_1_TACACACTTTGCGA


title: 10X36_1_TAAGTCCTGACGTT


title: 10X36_1_TAAGGGCTGTAAAG


title: 10X36_1_TAAGCGTGTTCGCC


title: 10X36_1_TAATCGCTCTTGAG


title: 10X36_1_TAAGAACTTAGTCG


title: 10X36_1_TACTTGACTCGTGA


title: 10X36_1_TAACTCACAGGTCT


title: 10X36_1_TAAGATTGTCTTAC


title: 10X36_1_TAAGGCTGACCTGA


title: 10X36_1_TAACTAGAATAAGG


title: 10X36_1_TAACCGGAGCTAAC


title: 10X36_1_TAACGTCTGAGGAC


title: 10X36_1_TAAAGACTTTTCAC


title: 10X36_1_TAACAATGTAGCGT


title: 10X36_1_TAATGAACGGGACA


title: 10X36_1_GTTTAAGATCTCTA


title: 10X36_1_GTTTAAGATAGAGA


title: 10X36_1_GTTGAGTGCGTAGT


title: 10X36_1_GTTGAGTGACCATG


title: 10X36_1_TAAGTAACCGTGTA


title: 10X36_1_GTTATGCTTCGCAA


title: 10X36_1_GTTATCTGTCTTTG


title: 10X36_1_GTTATAGAGCGATT


title: 10X36_1_GTTATCTGTAGAGA


title: 10X36_1_GTTATAGAAGACTC


title: 10X36_1_TAAGAACTCGTGTA


title: 10X36_1_GTTAAAACCTACTT


title: 10X36_1_GTTACTACACTGGT


title: 10X36_1_GTGTCAGAGGAGGT


title: 10X36_1_GTGTACGATCAAGC


title: 10X36_1_GTGTACGACCTTGC


title: 10X36_1_GTGTAGTGCTCGCT


title: 10X36_1_GTGCTAGATCTATC


title: 10X36_1_GTGATTCTAGCTAC


title: 10X36_1_GTTTAAGAGACGAG


title: 10X36_1_GTGATCGATCCGTC


title: 10X36_1_GTTGTACTCTCGCT


title: 10X36_1_GTTGATCTATTCTC


title: 10X36_1_GTGAGGGACAAGCT


title: 10X36_1_GTGATCGAAATGCC


title: 10X36_1_GTTGACGAAGCTCA


title: 10X36_1_GTGAGGGAATGCTG


title: 10X36_1_GTGACAACCATTCT


title: 10X36_1_TCCAGAGAAGGAGC


title: 10X36_1_GTCTGAGAAAAACG


title: 10X36_1_GTCTGAGAGGATTC


title: 10X36_1_GTCGACCTGCTGTA


title: 10X36_1_GTCGACCTAACAGA


title: 10X36_1_GTTAGTCTCTGGAT


title: 10X36_1_GTCATACTCTCATT


title: 10X36_1_GTCCCATGGATGAA


title: 10X36_1_GTCACCTGTTGCAG


title: 10X36_1_GTCCAGCTATCGAC


title: 10X36_1_GTCAATCTTAAGGA


title: 10X36_1_GTTAAATGCGCATA


title: 10X36_1_GTCAATCTCTAGAC


title: 10X36_1_GTATTCACCTGTGA


title: 10X36_1_GTATTAGATTAGGC


title: 10X36_1_GTAGTGTGGGACAG


title: 10X36_1_GTATTCACGAGGGT


title: 10X36_1_GTCAACGATCGCAA


title: 10X36_1_GTGATCGAGCTACA


title: 10X36_1_GTAGTGTGACACCA


title: 10X36_1_GTAGTCGAAAAAGC


title: 10X36_1_GTAGCAACACTCTT


title: 10X36_1_GTAGCCCTGTACAC


title: 10X36_1_GTACTTTGACACGT


title: 10X36_1_GTACTTTGCGGAGA


title: 10X36_1_GTACTTTGGCGAGA


title: 10X36_1_GTACCCTGCTACGA


title: 10X36_1_GTACTTTGGTTCGA


title: 10X36_1_GTACGAACGGTGGA


title: 10X36_1_GTACAGTGCAGAGG


title: 10X36_1_GTAATAACCCAACA


title: 10X36_1_GTAAGCACAGCTCA


title: 10X36_1_GGTTGAACGGTTTG


title: 10X36_1_GGTTTACTTGGATC


title: 10X36_1_GGTGATACAGAAGT


title: 10X36_1_GGTCTAGAACGGGA


title: 10X36_1_GGTCAAACTAGTCG


title: 10X36_1_GGTCAAACCCAATG


title: 10X36_1_GGTACAACCTCTTA


title: 10X36_1_GGGTTATGCCTTAT


title: 10X36_1_GTAGTGTGGCATCA


title: 10X36_1_GGGTTAACGGAGGT


title: 10X36_1_GGGTTATGGAGGGT


title: 10X36_1_GTAATATGCTTCTA


title: 10X36_1_GGGTAACTTCCTCG


title: 10X36_1_GGGTTAACTTCATC


title: 10X36_1_GGTCAAACCACTAG


title: 10X36_1_GGGCCATGCAACTG


title: 10X36_1_GGGCCATGCCCGTT


title: 10X36_1_GGGCCAACGCAGAG


title: 10X36_1_GGGCAAGATTTCGT


title: 10X36_1_GGGACCACCATACG


title: 10X36_1_GGCTCACTTGGTAC


title: 10X36_1_GGCTACCTTACGAC


title: 10X36_1_GGCTAATGTTCACT


title: 10X36_1_GGCTACCTTGGAAA


title: 10X36_1_GGCTAATGGGGTGA


title: 10X36_1_GGCTACCTCTGTTT


title: 10X36_1_GGCTAATGACGGAG


title: 10X36_1_GGCGCATGTTCGTT


title: 10X36_1_GGCGGACTAGGAGC


title: 10X36_1_GGTGGAGACCTCGT


title: 10X36_1_GGCGACTGATTCTC


title: 10X36_1_GGCGCATGGCCCTT


title: 10X36_1_GGCCCAGAAAGTAG


title: 10X36_1_GGTAAAGACGTAAC


title: 10X36_1_GGCCAGACGAGACG


title: 10X36_1_GGATGTTGTGGGAG


title: 10X36_1_GGATACTGGGTACT


title: 10X36_1_GGATTTCTGACACT


title: 10X36_1_GGATGTTGATCGAC


title: 10X36_1_GGCCACGACCGTAA


title: 10X36_1_GGCACGTGTAGCCA


title: 10X36_1_GGAGTTTGCTATTC


title: 10X36_1_GGAGTTTGCCTAAG


title: 10X36_1_GGAGTTTGGGACAG


title: 10X36_1_GGAGTTTGTATCTC


title: 10X36_1_GGAGGCCTTTCACT


title: 10X36_1_GGAGGCCTAATCGC


title: 10X36_1_GGAGGTGACTGTAG


title: 10X36_1_GGAGCGCTGACGGA


title: 10X36_1_GGAGAGACTCCAAG


title: 10X36_1_GGAGAGACCTCCAC


title: 10X36_1_GGAGAGACCCATGA


title: 10X36_1_GGACGCACCTTGCC


title: 10X36_1_GGCCGAACCTCATT


title: 10X36_1_GGACGCACCTCAAG


title: 10X36_1_GGCCGATGACGGAG


title: 10X36_1_GGACGCTGCACACA


title: 10X36_1_GGACGAGAAGGGTG


title: 10X36_1_GGACCGTGCTCGAA


title: 10X36_1_GGACCCGATAGACC


title: 10X36_1_GGCAATACCCACCT


title: 10X36_1_GGACCCGACTCGCT


title: 10X36_1_GGACCGTGCGGGAA


title: 10X36_1_GGACCCGACCTCAC


title: 10X36_1_GGACATTGGTAGGG


title: 10X36_1_GGAGTTTGGCCAAT


title: 10X36_1_GGAAGGTGGGTATC


title: 10X36_1_GGACATTGTCTCCG


title: 10X36_1_GGAATCTGCCCGTT


title: 10X36_1_GGAACTACGAATCC


title: 10X36_1_GCTTGAGATGGGAG


title: 10X36_1_GCTTAACTTGCCTC


title: 10X36_1_GCTGATGAGAAGGC


title: 10X36_1_GCTCGACTGGGATG


title: 10X36_1_GCTTGAGATATCGG


title: 10X36_1_GCTCGACTGGTAAA


title: 10X36_1_GCTCGACTAAAACG


title: 10X36_1_GCTCAGCTGACGGA


title: 10X36_1_GCTACGCTTCATTC


title: 10X36_1_GCTACCTGCAATCG


title: 10X36_1_GCTAGATGCTTGCC


title: 10X36_1_GCTATACTCCAGTA


title: 10X36_1_GCTACAGACCCAAA


title: 10X36_1_GCGTAATGGCTAAC


title: 10X36_1_GCGTACCTGCTCCT


title: 10X36_1_GCTACAGACAGCTA


title: 10X36_1_GCGTAATGACAGCT


title: 10X36_1_GCGTAAACAGTCTG


title: 10X36_1_GCGGGACTGGTGAG


title: 10X36_1_GCGCGATGTGGTGT


title: 10X36_1_GCGCGATGGCCTTC


title: 10X36_1_GCGGACTGAGTGCT


title: 10X36_1_GCGCGAACTTTCAC


title: 10X36_1_GGAAGGACCTCTCG


title: 10X36_1_GCGCGAACCACACA


title: 10X36_1_GCGAGCACAGTGTC


title: 10X36_1_GCGAAGGAGTGCAT


title: 10X36_1_GCGAGCACTGCCCT


title: 10X36_1_GCCTACACTCCTCG


title: 10X36_1_GCCGTACTTTTACC


title: 10X36_1_GCCTAGCTGACACT


title: 10X36_1_GCCGAGTGTCTACT


title: 10X36_1_GCCGTACTGACAGG


title: 10X36_1_GCCTCATGCGTAAC


title: 10X36_1_GCCCATACCTTGCC


title: 10X36_1_GCCCAACTTCCCGT


title: 10X36_1_GCCACGGATCTTAC


title: 10X36_1_GCCAAATGGATAGA


title: 10X36_1_GCCAAAACCTAAGC


title: 10X36_1_GCATTGGAATCGAC


title: 10X36_1_GCCAAATGCGGTAT


title: 10X36_1_GCAGGGCTCAGCTA


title: 10X36_1_GCAGGGCTACTGTG


title: 10X36_1_GCAGGCACTGCATG


title: 10X36_1_GCAGCTCTTCTCAT


title: 10X36_1_GCAGCCGAACACCA


title: 10X36_1_GCACTGCTTGAGCT


title: 10X36_1_GCAGATACTCTTCA


title: 10X36_1_GCCTCATGGAAACA


title: 10X36_1_GCACCACTGCGAAG


title: 10X36_1_GCACTAGATGTCGA


title: 10X36_1_GCACGGACCTACGA


title: 10X36_1_GCAAGACTTTGGCA


title: 10X36_1_GCAAGACTTGTTCT


title: 10X36_1_GCAATCGATCCAGA


title: 10X36_1_GCCGACGACGTCTC


title: 10X36_1_GCAAGACTGTGCAT


title: 10X36_1_GCAAACTGGGATTC


title: 10X36_1_GATTTGCTTACAGC


title: 10X36_1_GCAACCCTCTGTGA


title: 10X36_1_GATTTGCTAGCAAA


title: 10X36_1_GATTTGCTAAGATG


title: 10X36_1_GCAGTCCTTTCTGT


title: 10X36_1_GATTGGTGAAAACG


title: 10X36_1_GATTACCTTTCCGC


title: 10X36_1_GATTACCTGTATGC


title: 10X36_1_GATGCCCTACTGTG


title: 10X36_1_GATGCCCTCTAGTG


title: 10X36_1_GATCTTTGAGCTAC


title: 10X36_1_GATCTTACGAGGCA


title: 10X36_1_GATCTTACGGGACA


title: 10X36_1_GATCTTACACCCTC


title: 10X36_1_GATCCCTGTTGGTG


title: 10X36_1_GATCCGCTTCTATC


title: 10X36_1_GATCCCTGTGCCAA


title: 10X36_1_GATATTGAAGAGAT


title: 10X36_1_GATATCCTTAAAGG


title: 10X36_1_GATCGAACCTTGTT


title: 10X36_1_GATATCCTATCGGT


title: 10X36_1_GATATCCTGGCGAA


title: 10X36_1_GATTTGCTACGTAC


title: 10X36_1_GATATCCTGTCTGA


title: 10X36_1_GATATCCTAGCTCA


title: 10X36_1_GAGTGTTGCGATAC


title: 10X36_1_GAGTGGGACGGAGA


title: 10X36_1_GATTCTTGACACGT


title: 10X36_1_GATAATACGGTCAT


title: 10X36_1_GAGTACTGTTCCCG


title: 10X36_1_GAGTGACTAGGGTG


title: 10X36_1_GAGTACACACGCTA


title: 10X36_1_GAGTAAGATATTCC


title: 10X36_1_GAGTACACTCTTAC


title: 10X36_1_GATCTACTTAAAGG


title: 10X36_1_GAGGTGGAAGTGCT


title: 10X36_1_GAGGCAGATTGTGG


title: 10X36_1_GATCCGCTGTAAGA


title: 10X36_1_GAGGGATGTAAGGA


title: 10X36_1_GAGCGGCTGAAGGC


title: 10X36_1_GAGCGCTGTTGCGA


title: 10X36_1_GAGGATCTCATGCA


title: 10X36_1_GAGCTCCTGTAGGG


title: 10X36_1_GAGCGCACGTAAGA


title: 10X36_1_GATAATACCGGAGA


title: 10X36_1_GAGCGCTGGAGCTT


title: 10X36_1_GAGAAATGACTTTC


title: 10X36_1_GAGAAATGTGCTTT


title: 10X36_1_GAGAGGTGTTGAGC


title: 10X36_1_GAGCAACTTGAACC


title: 10X36_1_GAGCGAGACGTTAG


title: 10X36_1_GACTTTACTCGACA


title: 10X36_1_GAGATAGAATCTTC


title: 10X36_1_GACTGTGATTGCTT


title: 10X36_1_GACTTTACTGACTG


title: 10X36_1_GACTTTACAGAGGC


title: 10X36_1_GACTGATGGAAACA


title: 10X36_1_GACTCCTGCCGTAA


title: 10X36_1_GACTGATGCTTAGG


title: 10X36_1_GACGATTGACTCTT


title: 10X36_1_GACGAGGAGTTGAC


title: 10X36_1_GACGAGGATTAGGC


title: 10X36_1_GACCTAGACCCGTT


title: 10X36_1_GACAGTTGTCCAGA


title: 10X36_1_GACAGTTGTCAGGT


title: 10X36_1_GACCTCTGAGTCGT


title: 10X36_1_GACAGTACTCTCCG


title: 10X36_1_GACACTGATCGATG


title: 10X36_1_GACAGTACTCCTAT


title: 10X36_1_GAGCGCTGGAATGA


title: 10X36_1_GAATGGCTCGTAGT


title: 10X36_1_GACAGTACATCGTG


title: 10X36_1_GAGAGGTGTGGAGG


title: 10X36_1_GAATGGCTCGACTA


title: 10X36_1_GAAGTCACGTCTAG


title: 10X36_1_GAAGTCTGCAAGCT


title: 10X36_1_GAAGCGGAGTCATG


title: 10X36_1_GAAGTCACACGTAC


title: 10X36_1_GAAGTGCTAACTGC


title: 10X36_1_GAAGATGAACCTGA


title: 10X36_1_GAAGAATGTCCTGC


title: 10X36_1_GAACGTTGGTTACG


title: 10X36_1_GAACTGTGTGCATG


title: 10X36_1_GAACCAACTGTCTT


title: 10X36_1_GAACCAACCTGTAG


title: 10X36_1_GAACAGCTCAGTCA


title: 10X36_1_CTTTGATGACACAC


title: 10X36_1_GAACGTTGGATAGA


title: 10X36_1_GAAAGCCTCATGGT


title: 10X36_1_CTTTAGTGTTCTGT


title: 10X36_1_GAAAGCCTGCGAAG


title: 10X36_1_CTTTGATGCCACAA


title: 10X36_1_CTTTAGACAGCCAT


title: 10X36_1_CTTCTAGACTTGCC


title: 10X36_1_CTTGAGGATTCTAC


title: 10X36_1_CTTGATTGAAGGTA


title: 10X36_1_CTTTACGATTTGGG


title: 10X36_1_GAAGCTTGACCTCC


title: 10X36_1_CTTCATGATCGCTC


title: 10X36_1_CTTAGGGACCTATT


title: 10X36_1_GAACGTTGCCATAG


title: 10X36_1_CTTAAGCTCCTCCA


title: 10X36_1_CTTCATGAGGTGTT


title: 10X36_1_CTTCACCTTGTCAG


title: 10X36_1_CTTACAACCCACAA


title: 10X36_1_CTTAAAGATAGCGT


title: 10X36_1_GAAGTCTGCTCTTA


title: 10X36_1_CTGGATGACGCAAT


title: 10X36_1_CTTAAAGATAGACC


title: 10X36_1_CTGTGAGATCGATG


title: 10X36_1_CTTTAGACTTTCGT


title: 10X36_1_CTGGAAACTGCCTC


title: 10X36_1_CTGGAAACGAGGAC


title: 10X36_1_CTGATGGACTGACA


title: 10X36_1_CTGCAGCTAGGCGA


title: 10X36_1_CTGATACTTATTCC


title: 10X36_1_CTGATGGATTCCAT


title: 10X36_1_GAAGAATGAGATCC


title: 10X36_1_CTGAGCCTGCTATG


title: 10X36_1_CTTTGATGGGTGAG


title: 10X36_1_CTGATACTTGAGCT


title: 10X36_1_CTTACATGAAGTGA


title: 10X36_1_CTGAGAACGGTTAC


title: 10X36_1_CTGACCACTAGACC


title: 10X36_1_CTGACCACCCTTGC


title: 10X36_1_CTGATACTCTTCGC


title: 10X36_1_CTGAATCTTGCAAC


title: 10X36_1_CTGAATCTCTCAAG


title: 10X36_1_CTGACAGATCTTAC


title: 10X36_1_CTCCTACTCTGGAT


title: 10X36_1_CTCCTACTGGTACT


title: 10X36_1_CTCCGAACCACCAA


title: 10X36_1_CTCGAAGAAACCTG


title: 10X36_1_CTCAGCACTGATGC


title: 10X36_1_CTCAATTGAAAACG


title: 10X36_1_CTATTGTGTCTGGA


title: 10X36_1_CTCAGCACCTGTGA


title: 10X36_1_CTATTGTGCTACTT


title: 10X36_1_CTATGTTGGGGACA


title: 10X36_1_CTATGTTGTGAACC


title: 10X36_1_CTGAGAACAAGGCG


title: 10X36_1_CTATGACTATTCCT


title: 10X36_1_CTATCCCTGTACGT


title: 10X36_1_CTATCAACAGGTCT


title: 10X36_1_CTATAGCTCGTAAC


title: 10X36_1_CTATAAGATACGCA


title: 10X36_1_CTCGAAGACGTAAC


title: 10X36_1_CTCCGAACTGACAC


title: 10X36_1_CTAGGCCTCTATGG


title: 10X36_1_CTATAAGAGAAAGT


title: 10X36_1_CTAGATCTCTGTCC


title: 10X36_1_CTAGAGACTAGCGT


title: 10X36_1_CTGTATACGCGAAG


title: 10X36_1_CTCCACGATGGTAC


title: 10X36_1_CTCCATCTTATTCC


title: 10X36_1_CTAGGCCTAGTCAC


title: 10X36_1_CTAATAGAGTGTTG


title: 10X36_1_CTAACACTGAAGGC


title: 10X36_1_CTAGAGACTCCTAT


title: 10X36_1_CTACCTCTAGCTCA


title: 10X36_1_CGTGTAGAGTAGCT


title: 10X36_1_CGTTAGGATTCACT


title: 10X36_1_CGTTATACTCTGGA


title: 10X36_1_CTATGACTTAGAAG


title: 10X36_1_CGTGTAGAGGGAGT


title: 10X36_1_CGTGTAGAGAGATA


title: 10X36_1_CTATCCCTAGAACA


title: 10X36_1_CTATCAACTTGGCA


title: 10X36_1_CGTGATGAGACGGA


title: 10X36_1_CGTGAATGGTGTTG


title: 10X36_1_CGTGAATGCGCTAA


title: 10X36_1_CGTGAATGCAAGCT


title: 10X36_1_CGTCGACTTGCAAC


title: 10X36_1_CGTCGACTACACTG


title: 10X36_1_CGTCGACTAACCTG


title: 10X36_1_CTACTCCTAGTAGA


title: 10X36_1_CGTCCAACTCCCGT


title: 10X36_1_CGTCCATGCCGTTC


title: 10X36_1_CGTCCAACCTCAAG


title: 10X36_1_CGTCCAACCACTTT


title: 10X36_1_CGTACCTGCAGGAG


title: 10X36_1_CGTACCACTGCTCC


title: 10X36_1_CGGGACTGACGACT


title: 10X36_1_CGTAACGAATAAGG


title: 10X36_1_CGGCGATGGCGAAG


title: 10X36_1_CGGCGATGAGGGTG


title: 10X36_1_CGGGCATGGGTAAA


title: 10X36_1_CGCTAAGACTGGTA


title: 10X36_1_CGGACCGATAGACC


title: 10X36_1_CGGATAACCGCAAT


title: 10X36_1_CGGCCAGAATTCGG


title: 10X36_1_CGCGGATGTGGGAG


title: 10X36_1_CGGATATGCAAGCT


title: 10X36_1_CGCTACTGTTCTGT


title: 10X36_1_CGCGAGACCCTAAG


title: 10X36_1_CGCGAGACGATGAA


title: 10X36_1_CGCGATCTCTCATT


title: 10X36_1_CGGCACGATCACGA


title: 10X36_1_CGCCGAGACGTTAG


title: 10X36_1_CGCCATACCCTTCG


title: 10X36_1_CGCAGGTGGAGAGC


title: 10X36_1_CGCACTACGTCGTA


title: 10X36_1_CGCACGGATGTCCC


title: 10X36_1_CGCAACCTGTGTTG


title: 10X36_1_CGAGGGCTATCGAC


title: 10X36_1_CGCAAATGGAGGCA


title: 10X36_1_CGTACCTGAACCTG


title: 10X36_1_CGATAGACGCTGTA


title: 10X36_1_CGCAAATGTAGCGT


title: 10X36_1_CGATCCACCCTTTA


title: 10X36_1_CGAGGAGATTGGTG


title: 10X36_1_CGACTGCTAACGGG


title: 10X36_1_CGAGAACTCTGTCC


title: 10X36_1_CGGAATTGCGCTAA


title: 10X36_1_CGACTCACCCACAA


title: 10X36_1_CGACCTTGGATGAA


title: 10X36_1_CGACTCACGCAGAG


title: 10X36_1_CGAAGGGAGCATAC


title: 10X36_1_CCTTTAGACTTGAG


title: 10X36_1_CGACCTTGAGATGA


title: 10X36_1_CGACAAACCCATAG


title: 10X36_1_CCTTAATGACTACG


title: 10X36_1_CGCACTTGCGCAAT


title: 10X36_1_CCTTAATGCCTTCG


title: 10X36_1_CGAATCGAATCGTG


title: 10X36_1_CGACGTCTAATGCC


title: 10X36_1_CCTCTACTTCATTC


title: 10X36_1_CCTATAACCTACCC


title: 10X36_1_CCTTAATGCAACCA


title: 10X36_1_CCTAGAGAACGGAG


title: 10X36_1_CCTCATCTGTTCAG


title: 10X36_1_CCTGCAACCTGTGA


title: 10X36_1_CCGTAAGACTGAGT


title: 10X36_1_CCGATAGATTAGGC


title: 10X36_1_CCGGTACTCCACCT


title: 10X36_1_CCGGAGACGTTGCA


title: 10X36_1_CCCTTACTCTGACA


title: 10X36_1_CGACTCACAAGAAC


title: 10X36_1_CCCTGAACTCTTTG


title: 10X36_1_CGAAGTACAGCACT


title: 10X36_1_CCCGGAGACCAACA


title: 10X36_1_CCCTACGATCTTTG


title: 10X36_1_CCCGATTGGCATCA


title: 10X36_1_CCCGATTGCATTCT


title: 10X36_1_CCCGATTGAGAACA


title: 10X36_1_CCCAACACAAGCCT


title: 10X36_1_CCCAAAGAGAGGGT


title: 10X36_1_CCCAGACTTCTCCG


title: 10X36_1_CCCAAAGAAGAGTA


title: 10X36_1_CCCAAAGACTGATG


title: 10X36_1_CCATTAACGACTAC


title: 10X36_1_CCATGCTGCTGTCC


title: 10X36_1_CCTAAACTACCCTC


title: 10X36_1_CCTAAGGACATGAC


title: 10X36_1_CCATCGTGTCCGTC


title: 10X36_1_CCGACTACAGCCAT


title: 10X36_1_CCATATACATCAGC


title: 10X36_1_CCATAGGAGATAAG


title: 10X36_1_CCAGGTCTAGGGTG


title: 10X36_1_CCATAGGACATCAG


title: 10X36_1_CCATCGTGTGGAGG


title: 10X36_1_CCAGCGGAGTATCG


title: 10X36_1_CCAGCGGACCTTGC


title: 10X36_1_CCAGTGCTGCTTAG


title: 10X36_1_CCAGCACTCGTCTC


title: 10X36_1_CCAGATGAAGGCGA


title: 10X36_1_CCAGACCTAGGTTC


title: 10X36_1_CCACTTCTTCGCTC


title: 10X36_1_CCAGAAACACGCTA


title: 10X36_1_CCAGACCTCATGAC


title: 10X36_1_CCCATCGACGCCTT


title: 10X36_1_CCACCATGCTCGCT


title: 10X36_1_CCAATGGAGTTTGG


title: 10X36_1_CCACTGACTGGATC


title: 10X36_1_CATTGACTAACTGC


title: 10X36_1_CCAAGTGAAAACAG


title: 10X36_1_CATGTTTGGCGGAA


title: 10X36_1_CATTAGCTGGCATT


title: 10X36_1_CATGTTTGCGTACA


title: 10X36_1_CATTCCCTCGCATA


title: 10X36_1_CCAGATGAGGTTCA


title: 10X36_1_CATGTTACCCGAAT


title: 10X36_1_CATGCGCTGGTGTT


title: 10X36_1_CATCTTGACCTTAT


title: 10X36_1_CATCGGCTTTGACG


title: 10X36_1_CATGCGCTGTCACA


title: 10X36_1_CATGCGCTCCAATG


title: 10X36_1_CATGAGACTACGAC


title: 10X36_1_CATCTTGAAAAGTG


title: 10X36_1_CATCGGCTAAAAGC


title: 10X36_1_CATCAACTTAGCCA


title: 10X36_1_CATCAACTTGTGAC


title: 10X36_1_CATCGCTGAGTACC


title: 10X36_1_CATCAACTTAGACC


title: 10X36_1_CATAGTCTTCGACA


title: 10X36_1_CATAGTCTGGTCAT


title: 10X36_1_CATAGTCTCTTGCC


title: 10X36_1_CATATAGACAGATC


title: 10X36_1_CATACTTGTCCTCG


title: 10X36_1_CATACTACGCAGAG


title: 10X36_1_CCAAGATGACACCA


title: 10X36_1_CCACGGGAGGATCT


title: 10X36_1_CATAACCTCATGCA


title: 10X36_1_CATACTACCCTTAT


title: 10X36_1_CATAAAACATAAGG


title: 10X36_1_CATAAAACCTACGA


title: 10X36_1_CAGTCAGATTCCGC


title: 10X36_1_CAGTGATGGTACGT


title: 10X36_1_CAGTCAGAGACTAC


title: 10X36_1_CAGGTTGACAAAGA


title: 10X36_1_CAGGTATGGAATCC


title: 10X36_1_CAGGTAACCGTAGT


title: 10X36_1_CAGGCCGAAGGTTC


title: 10X36_1_CAGCCTTGCCCACT


title: 10X36_1_CAGCTCACGTTCTT


title: 10X36_1_CAGCTCACCTATGG


title: 10X36_1_CAGGCCGAGTTAGC


title: 10X36_1_CAGGTATGAAAGCA


title: 10X36_1_CAGCCTACGTAAAG


title: 10X36_1_CAGCATGAGTAAAG


title: 10X36_1_CAGCACCTCTCGAA


title: 10X36_1_CAGATCGAGTCACA


title: 10X36_1_CAGACTGAAGATCC


title: 10X36_1_CAGCAATGAGCAAA


title: 10X36_1_CAGACCCTGGTAAA


title: 10X36_1_CAGACAACTGCGTA


title: 10X36_1_CAGACCCTGCAAGG


title: 10X36_1_CAGACCCTCTACGA


title: 10X36_1_CAGACAACATGCCA


title: 10X36_1_CACTTTGATTCGTT


title: 10X36_1_CAGTTTACCATTGG


title: 10X36_1_CACTTAACGCTGTA


title: 10X36_1_CACTGCACTACTGG


title: 10X36_1_CACTTAACCGATAC


title: 10X36_1_CACTGCTGCTACTT


title: 10X36_1_CACTGAGAGGAAGC


title: 10X36_1_CACTGAGAGGTACT


title: 10X36_1_CACTAGGACACTCC


title: 10X36_1_CACTAACTTTCATC


title: 10X36_1_CACTAACTGAAACA


title: 10X36_1_CACTAACTAACGTC


title: 10X36_1_CACGGGTGGGGAGT


title: 10X36_1_CACGCTACATCGAC


title: 10X36_1_CACGGGACCCCTCA


title: 10X36_1_CACGAAACTTGGCA


title: 10X36_1_CACGATGAATCTTC


title: 10X36_1_CACCGGGATAAGCC


title: 10X36_1_CACCACTGTGTTCT


title: 10X36_1_CACATACTTGGTGT


title: 10X36_1_CACCACTGCTACGA


title: 10X36_1_CACAGTGAGGTAGG


title: 10X36_1_CACATACTGAGGCA


title: 10X36_1_CACAATCTGGGCAA


title: 10X36_1_CACAGTGAGTTCTT


title: 10X36_1_CACAACGATCGTTT


title: 10X36_1_CACAACGAGTTACG


title: 10X36_1_CACTTATGTATGGC


title: 10X36_1_CACAACGAAATGCC


title: 10X36_1_CACTTTGATGCCTC


title: 10X36_1_CAATTCTGGTGTAC


title: 10X36_1_CAATTCACAACCTG


title: 10X36_1_CACTATACCTGAAC


title: 10X36_1_CAATCTACCGTGTA


title: 10X36_1_CAATAATGGACGTT


title: 10X36_1_CAATCGGATTCGGA


title: 10X36_1_CACGGGTGTGGTGT


title: 10X36_1_CAATAAACTGTGGT


title: 10X36_1_CAATAAACGACAGG


title: 10X36_1_CAAGCCCTTTGCGA


title: 10X36_1_CAAGCTGACCTTCG


title: 10X36_1_CAATAAACGAATCC


title: 10X36_1_CAAGCCCTCTCAGA


title: 10X36_1_CAAGCCCTATCGAC


title: 10X36_1_CAAGTTCTACCGAT


title: 10X36_1_CAAGACACCAGAGG


title: 10X36_1_CAAGACACAGAGAT


title: 10X36_1_CAAGAAGACCACAA


title: 10X36_1_CAACTTTGACTTTC


title: 10X36_1_CAACTTTGCGGTAT


title: 10X36_1_CAACGAACAACCAC


title: 10X36_1_CAACTTTGAACGTC


title: 10X36_1_CAACCGCTCTCCCA


title: 10X36_1_CAACAGACTTCTCA


title: 10X36_1_CAACAGACGGACGA


title: 10X36_1_CAACAGACGCAAGG


title: 10X36_1_CAATGGACCCCTTG


title: 10X36_1_CAAATATGCTAGCA


title: 10X36_1_CAAATATGCTCTAT


title: 10X36_1_CAAATATGAGCGTT


title: 10X36_1_CAAACTCTGAGAGC


title: 10X36_1_ATTTCTCTTTCGTT


title: 10X36_1_ATTTGCACGAGACG


title: 10X36_1_CAACGAACAGTGCT


title: 10X36_1_CAAGACACCCGATA


title: 10X36_1_ATTTCGTGCTCTAT


title: 10X36_1_ATTGGTCTTCCTTA


title: 10X36_1_ATTTCGTGTATTCC


title: 10X36_1_ATTTAGGACATGAC


title: 10X36_1_ATTGGTCTTACTGG


title: 10X36_1_CAAGTTCTGTCTTT


title: 10X36_1_ATTGGTCTAGTTCG


title: 10X36_1_ATTGGGTGGCTTAG


title: 10X36_1_ATTTCCGACTGCAA


title: 10X36_1_ATTGCTTGCCACAA


title: 10X36_1_ATTGCGGATCCAAG


title: 10X36_1_ATTGCTACACCTCC


title: 10X36_1_ATTGCGGACATGCA


title: 10X36_1_ATTGCACTTTGTGG


title: 10X36_1_ATTGCTACGAGAGC


title: 10X36_1_ATTGATGATGAAGA


title: 10X36_1_ATTGATGACCAATG


title: 10X36_1_ATTGATGAATTGGC


title: 10X36_1_ATTGAATGGTTAGC


title: 10X36_1_ATTGATGAAAGTAG


title: 10X36_1_ATTGAATGCAGCTA


title: 10X36_1_ATTGAAACTGGTCA


title: 10X36_1_ATTCGACTAATGCC


title: 10X36_1_ATTGAAACCGTTGA


title: 10X36_1_ATTCAAGAAAACAG


title: 10X36_1_ATTAGTGAAGCCAT


title: 10X36_1_ATTATGGAAGTCAC


title: 10X36_1_ATTACCTGTGACTG


title: 10X36_1_ATTACCTGATTGGC


title: 10X36_1_ATTAGATGGGTAGG


title: 10X36_1_ATTAAGACGAGATA


title: 10X36_1_ATTGGTCTCTTCCG


title: 10X36_1_ATGTTCACAGGTTC


title: 10X36_1_ATGTTAGACTGCTC


title: 10X36_1_ATTGGGTGCAACTG


title: 10X36_1_ATGTTCACTTTCGT


title: 10X36_1_ATGTTGCTCCTGTC


title: 10X36_1_ATGTCACTGTTCAG


title: 10X36_1_ATGTACCTCTGGAT


title: 10X36_1_ATGGACACTAACGC


title: 10X36_1_ATTTAGGAGTTGCA


title: 10X36_1_ATGTAAACGATAAG


title: 10X36_1_ATGCTTTGGTTGGT


title: 10X36_1_ATTCAGCTTTTGCT


title: 10X36_1_ATGCAGTGGAAACA


title: 10X36_1_ATGCCAGACCATAG


title: 10X36_1_ATGCACGACCCTCA


title: 10X36_1_ATGCAGACAACGTC


title: 10X36_1_ATGCGATGATGTCG


title: 10X36_1_ATGAAGGATGTAGC


title: 10X36_1_ATGCACGAGTTCGA


title: 10X36_1_ATGAGAGAGTTGCA


title: 10X36_1_ATCTGTTGTACGAC


title: 10X36_1_ATCTACACGGTAGG


title: 10X36_1_ATCTACTGTGCTGA


title: 10X36_1_ATCTGACTAAAAGC


title: 10X36_1_ATCGGTGATAGCCA


title: 10X36_1_ATCTGACTGGTCTA


title: 10X36_1_ATCTACACGGTCTA


title: 10X36_1_ATCGCGCTTGCAAC


title: 10X36_1_ATGTCACTCTTGAG


title: 10X36_1_ATCGAGTGAACGAA


title: 10X36_1_ATGCCGCTTCCAAG


title: 10X36_1_ATCCCGTGCCTTCG


title: 10X36_1_ATCGCAGACCTCCA


title: 10X36_1_ATCATCTGCATCAG


title: 10X36_1_ATCAACCTGCATCA


title: 10X36_1_ATATAGTGGTCATG


title: 10X36_1_ATAGTTGAGGACAG


title: 10X36_1_ATAGCGTGAGCCAT


title: 10X36_1_ATAGCTCTCACAAC


title: 10X36_1_ATACGGACACCAGT


title: 10X36_1_ATACCTTGCGTGAT


title: 10X36_1_ATAGATTGCGTAAC


title: 10X36_1_ATACCACTTATGCG


title: 10X36_1_ATCGTTTGCTTTAC


title: 10X36_1_ATACCACTAAACAG


title: 10X36_1_ATAATGACTCGTAG


title: 10X36_1_ATAATGACGTCTTT


title: 10X36_1_AGTTTGCTTGGAGG


title: 10X36_1_ATCCGCACCTGTAG


title: 10X36_1_ATAATGACAAGCCT


title: 10X36_1_ATAACAACTCAGAC


title: 10X36_1_AGTTTCACTGCCAA


title: 10X36_1_ATCACTACCGGGAA


title: 10X36_1_AGTTTCACTTGAGC


title: 10X36_1_AGTTTCACGTCTTT


title: 10X36_1_AGTTCTTGTTGTGG


title: 10X36_1_AGTTGTCTTTGCGA


title: 10X36_1_ATATGAACCGTAGT


title: 10X36_1_AGTTCTTGAGTCGT


title: 10X36_1_AGTTATGAAGTCAC


title: 10X36_1_AGTGTTCTCAACCA


title: 10X36_1_ATATAGTGCCATGA


title: 10X36_1_ATACCGGACGCCTT


title: 10X36_1_ATACCACTTGGAGG


title: 10X36_1_AGTGACTGGTAGCT


title: 10X36_1_AGTGCAACAGCTCA


title: 10X36_1_AGTGACTGTACTGG


title: 10X36_1_AGTCTTACGACGTT


title: 10X36_1_AGTGCAACCTGGTA


title: 10X36_1_AGTCTTACACGGAG


title: 10X36_1_AGTCACGAAACCTG


title: 10X36_1_ATACACCTGACGAG


title: 10X36_1_AGTATAACTAGACC


title: 10X36_1_AGTAGGCTAACGTC


title: 10X36_1_AGTAGAGAGGGACA


title: 10X36_1_AGTATCCTCCATAG


title: 10X36_1_AGTATAACTTTGGG


title: 10X36_1_AGTACGTGGCTAAC


title: 10X36_1_AGGTTCGACCAAGT


title: 10X36_1_AGGTGTTGGTTTCT


title: 10X36_1_AGTAATTGTTCATC


title: 10X36_1_AGGTCATGTTCGGA


title: 10X36_1_AGGTGTTGGCGAGA


title: 10X36_1_AGGTCATGGCGAGA


title: 10X36_1_AGGTACACAGCATC


title: 10X36_1_AGGTCATGTTCGCC


title: 10X36_1_AGGTCATGACGGGA


title: 10X36_1_AGTTATGATAACGC


title: 10X36_1_AGGGCCTGCAATCG


title: 10X36_1_AGGGCCACTAAGCC


title: 10X36_1_AGGGCCACTCCGAA


title: 10X36_1_AGGCTAACCCGTTC


title: 10X36_1_AGGGACGACCTATT


title: 10X36_1_AGGAGTCTTTCGTT


title: 10X36_1_AGGATGCTCCATGA


title: 10X36_1_AGGATAGATGCACA


title: 10X36_1_AGGGACGATTACTC


title: 10X36_1_AGGACTTGGAACCT


title: 10X36_1_AGGAATGAGTACCA


title: 10X36_1_AGGAGTCTTAGCCA


title: 10X36_1_AGGAACCTGAGATA


title: 10X36_1_AGGACACTACGGGA


title: 10X36_1_AGGAACCTCCTACC


title: 10X36_1_AGCTTTACGAGGGT


title: 10X36_1_AGGGTGGATGCCAA


title: 10X36_1_AGCGCCGAGACGAG


title: 10X36_1_AGCGGCACACCATG


title: 10X36_1_AGCGCCGACAGCTA


title: 10X36_1_AGCGCTCTATGCTG


title: 10X36_1_AGCCGTCTGTAGGG


title: 10X36_1_AGCCGTCTCCGTAA


title: 10X36_1_AGCCTCACTTTGGG


title: 10X36_1_AGCGGGCTCTGGAT


title: 10X36_1_AGCATTCTCAGAAA


title: 10X36_1_AGCGAACTTCTCAT


title: 10X36_1_AGCATTCTTGGGAG


title: 10X36_1_AGCACTGAAGATCC


title: 10X36_1_AGCATCGAGTGCTA


title: 10X36_1_AGGACTTGCAGATC


title: 10X36_1_AGCAACACTGTCCC


title: 10X36_1_AGCAACACGAGCAG


title: 10X36_1_AGATTAACTTCAGG


title: 10X36_1_AGATTCCTCAGTCA


title: 10X36_1_AGATCGTGAAACAG


title: 10X36_1_AGAGTGCTTTTGGG


title: 10X36_1_AGCGATTGAACCTG


title: 10X36_1_AGAGTGCTGATGAA


title: 10X36_1_AGAGTCACTGGTAC


title: 10X36_1_AGAGTCACCTCGAA


title: 10X36_1_AGAGCTACACTCTT


title: 10X36_1_AGAGCGGAGAATAG


title: 10X36_1_AGAGAAACTTGTCT


title: 10X36_1_AGACTGACTCTAGG


title: 10X36_1_AGAGAAACGGAGCA


title: 10X36_1_AGACTTCTTCTCAT


title: 10X36_1_AGACTTCTAACGTC


title: 10X36_1_AGACGTACTGCTGA


title: 10X36_1_AGACGTACGTCATG


title: 10X36_1_AGACCTGATCTTCA


title: 10X36_1_AGAATGGAATGGTC


title: 10X36_1_AGACCTGATGACCA


title: 10X36_1_AGACACACCAGATC


title: 10X36_1_AGATTAACGTGTAC


title: 10X36_1_AGCAAGCTTCAGAC


title: 10X36_1_AGAACGCTAGTGTC


title: 10X36_1_AGAATACTCCTGAA


title: 10X36_1_AGAACAGATATGGC


title: 10X36_1_AGAACGCTGAATGA


title: 10X36_1_AGAAAGTGCTGCAA


title: 10X36_1_AGAAAGTGGGTGGA


title: 10X36_1_AGACACACAAACAG


title: 10X36_1_ACTTTGTGACTGGT


title: 10X36_1_ACTTGTACCTATGG


title: 10X36_1_ACTTGGGAGTTAGC


title: 10X36_1_ACTTGGGAGTGCTA


title: 10X36_1_AGACTTCTAGAAGT


title: 10X36_1_AGAGTCACCTACTT


title: 10X36_1_AGAAAGTGCGGGAA


title: 10X36_1_ACTTCTGACGGAGA


title: 10X36_1_ACTGCCTGTCTTCA


title: 10X36_1_ACTGCCTGTTCATC


title: 10X36_1_ACTTAGCTGGCGAA


title: 10X36_1_ACTCTCCTCTGAAC


title: 10X36_1_ACTCTCCTCTGTGA


title: 10X36_1_ACTGTTACCATGAC


title: 10X36_1_ACTCGCACAGTCAC


title: 10X36_1_ACTGCCTGCTTTAC


title: 10X36_1_ACTATCACATCAGC


title: 10X36_1_ACTAGGTGTCGCAA


title: 10X36_1_ACTCCCGAATGTGC


title: 10X36_1_ACTAGGTGCTTAGG


title: 10X36_1_ACTAGGTGCCCAAA


title: 10X36_1_ACGTTGGAATCTCT


title: 10X36_1_ACGTCAGACCTTCG


title: 10X36_1_ACGTCAGAACAGTC


title: 10X36_1_AGATATTGACCGAT


title: 10X36_1_ACGGTCCTGCAAGG


title: 10X36_1_ACGTAGACGCGTAT


title: 10X36_1_ACGGTCCTACCCTC


title: 10X36_1_ACGGTCCTACCATG


title: 10X36_1_ACGTGATGCATCAG


title: 10X36_1_ACGGTATGGGTATC


title: 10X36_1_ACGGCGTGTTATCC


title: 10X36_1_ACGGGAGAAAGGTA


title: 10X36_1_ACGGAGGATGATGC


title: 10X36_1_ACTCGCACGTCTGA


title: 10X36_1_ACGGCTCTATGTCG


title: 10X36_1_ACGGAACTGTAAGA


title: 10X36_1_ACGGAACTCTGAAC


title: 10X36_1_ACTCCTCTCGTAAC


title: 10X36_1_ACGCTCACGAGGAC


title: 10X36_1_ACGGTAACTTTCAC


title: 10X36_1_ACGCCACTGGTTCA


title: 10X36_1_ACGCGGTGGAGCTT


title: 10X36_1_ACGCGGTGGTTCGA


title: 10X36_1_ACGCGGTGGCTACA


title: 10X36_1_ACGCTCACGCAGTT


title: 10X36_1_ACGCAATGCTTCTA


title: 10X36_1_ACGATTCTGGTACT


title: 10X36_1_ACGCAATGCCCGTT


title: 10X36_1_ACGATTCTAAGGTA


title: 10X36_1_ACGATTCTCACTAG


title: 10X36_1_ACGAGTACGGAAGC


title: 10X36_1_ACGACCCTTTCCAT


title: 10X36_1_ACGAACACGTCACA


title: 10X36_1_ACCTTTGAGATGAA


title: 10X36_1_ACGAACTGCAGTCA


title: 10X36_1_ACCTCGTGTAACGC


title: 10X36_1_ACCTATTGGTGCTA


title: 10X36_1_ACCTCGTGTTATCC


title: 10X36_1_ACGAAGCTGTATGC


title: 10X36_1_ACCTATTGCTTGGA


title: 10X36_1_ACCTTTGAAAGTGA


title: 10X36_1_ACCGCGGACGGGAA


title: 10X36_1_ACCCTCGAGGTAAA


title: 10X36_1_ACCCGTTGACTGGT


title: 10X36_1_ACCCGTTGCTCAGA


title: 10X36_1_ACCCGTACTCCTTA


title: 10X36_1_ACCCAGCTGTTTCT


title: 10X36_1_ACCCAGCTCTGTAG


title: 10X36_1_ACCCACTGCGTGTA


title: 10X36_1_ACCCAAGAGTGAGG


title: 10X36_1_ACATGGTGACACGT


title: 10X36_1_ACCAGCCTTGACCA


title: 10X36_1_ACCATTACTGTAGC


title: 10X36_1_ACCATTTGCTGGAT


title: 10X36_1_ACCACGCTTTCAGG


title: 10X36_1_ACAGTCGAGCTACA


title: 10X36_1_ACAGTCGAAGAAGT


title: 10X36_1_ACAGTGTGTGACCA


title: 10X36_1_ACAGACACGGTGAG


title: 10X36_1_ACAGGTACGTCTTT


title: 10X36_1_ACACGATGTAAAGG


title: 10X36_1_ACAGGTACGTTGTG


title: 10X36_1_ACCAACGAAGTGCT


title: 10X36_1_ACACGAACAGATGA


title: 10X36_1_ACAATAACATGTGC


title: 10X36_1_ACACAGACACGGTT


title: 10X36_1_ACAAGCACGTTAGC


title: 10X36_1_ACCACAGATACTTC


title: 10X36_1_ACAAATTGAGAACA


title: 10X36_1_ACAAAGGACTTGCC


title: 10X36_1_ACCAACGACTAGTG


title: 10X36_1_ACAAAGGACATACG


title: 10X36_1_ACAAGCACGTACCA


title: 10X36_1_AATTGTGAGGACTT


title: 10X36_1_AATTGATGGTCATG


title: 10X36_1_AATGGCTGCGCAAT


title: 10X36_1_AATGTTGACTTCCG


title: 10X36_1_AATGTTGACGCTAA


title: 10X36_1_AATGATACAGATGA


title: 10X36_1_ACAGCAACGGTCTA


title: 10X36_1_ACAGACACAAAACG


title: 10X36_1_AATGCGTGAACGGG


title: 10X36_1_AATCCTTGTGCTGA


title: 10X36_1_AATGAGGACAGCTA


title: 10X36_1_AATAGGGAACCTCC


title: 10X36_1_AATACTGATTCGGA


title: 10X36_1_AAGTTCCTAAGAGT


title: 10X36_1_AATCTCACTCCCAC


title: 10X36_1_AATATCGACAACTG


title: 10X36_1_ACAATCCTAGCCAT


title: 10X36_1_AAGTTATGGCGTTA


title: 10X36_1_AATATCGAAACGAA


title: 10X36_1_AAGTAACTCTTGCC


title: 10X36_1_AAGTATACTCAAGC


title: 10X36_1_AAGGTGCTGTATCG


title: 10X36_1_AAGTCCGAAGAGTA


title: 10X36_1_AAGGCTTGGCTTAG


title: 10X36_1_AAGGCTACTTTCTG


title: 10X36_1_AAGCCATGGCTTCC


title: 10X36_1_AAGCACTGTCACGA


title: 10X36_1_AAGCCAACGTCGTA


title: 10X36_1_AAGCCAACCCCAAA


title: 10X36_1_AAGCAAGAGTTTGG


title: 10X36_1_AAGAGATGCTATTC


title: 10X36_1_AAGCACTGCTGCAA


title: 10X36_1_AAGACAGATTACTC


title: 10X36_1_AAGAATCTGCGTAT


title: 10X36_1_AAGAACGACCGCTT


title: 10X36_1_AAGAAGACTTGCAG


title: 10X36_1_AACTGTCTCAGGAG


title: 10X36_1_AATACTGAAGGAGC


title: 10X36_1_AACTTGCTCGAACT


title: 10X36_1_AACTCACTCACTAG


title: 10X36_1_AATAACACGGAACG


title: 10X36_1_AACTCACTTCCCGT


title: 10X36_1_AACGTCGACCTCAC


title: 10X36_1_AACGTGTGCTACGA


title: 10X36_1_AACGCATGTGCCTC


title: 10X36_1_AACGGTTGAGTCTG


title: 10X36_1_AACCTTTGTTCTAC


title: 10X36_1_AACGCCCTTCGTAG


title: R60N-3

city: West Lafayette

first: Kranthi

last: Varala

state: IN

address: 625 Agriculture Mall Drive

department: Horticulture and Landscape Architecture

country: USA

institute: Purdue University

data_processing:
Raw reads were trimmed to remove low quality bases (q<10) and adapter contamination.
Gene expression levels were determined by aligning reads to the Arabidopsis thaliana genome (TAIR10) using the TopHat (v2) package and estimating read counts by the HTseq package.
Gene expression counts across the libraries were normalized using a quantile normalization method as implemented in the EDASeq R package
Genes responding to nitrogen significantly (FDR adjusted p-val <0.05) over the time-series N-response data were identified by fitting a Cubic Spline Model (df=5) to the N-treatment and Control samples, using the lmFit function in the Limma R package
Genome_build: TAIR10
Supplementary_files_format_and_content: Read counts output from HTSeq package

molecule: polyA RNA

organism: Arabidopsis thaliana

source_name: Seedling root

growth_protocol: Arabidopsis seedlings grown in phytatrays with 1% sucrose and 1mM KNO3

extract_protocol:
Approximately 100 mg of tissue was used to extract total RNA from pulverized, frozen roots and shoots with an RNeasy Mini Kit (Qiagen) with on-column DNase treatment. mRNA was purified using Dynabeads Oligo (dT).
Reverse transcription was performed using SuperScript III (Invitrogen, 18080-044) with second-strand synthesis with dUTPs, followed by cleanup, end repair, and dA-tailing. Universal Y-shaped adapter ligation was performed to enable sample multiplexing. Library amplification and multiplexing were performed using dUTP excision and amplification followed by PCR enrichment using 2x Phusion HF Master Mix (NEB, M0531L). Size-selection was performed using gel extraction and purification (Qiagen Gel Extraction Kit). Final quantity was determined using the DNA Bioanalyzer Chip and KAPA Library Quant qPCR.

treatment_protocol: Arabidopsis seedlings were treated with either 60 mM nitrogen (20 mM KNO3 + 20 mM NH4NO3) or 20 mM KCl

tag: organ

value: Root

tag: time


title: R120N-3


title: R90N-3


title: 10X36_1_AACGCAACCACTCC


title: 10X36_1_AACCTTTGGAGACG


title: 10X36_1_AACCTACTTGACAC


title: 10X36_1_AAGATTACAGAATG


title: R45N-3


title: R20N-3


title: 10X36_1_AACCCAGACCCAAA


title: 10X36_1_AACCACGATGCCCT


title: R10N-3


title: 10X36_1_AACAGCACTGCGTA


title: 10X36_1_AACTCTTGTCGTGA


title: R5N-3


title: R15N-3


title: 10X36_1_AACAGAGATAGACC


title: 10X36_1_AACAGAGAGGGACA


title: R90N-2


title: 10X36_1_AACAATACTGCACA


title: 10X36_1_AACAATACCCTGTC


title: R60N-2


title: R30N-2


title: 10X36_1_AAATGGGACGTAAC


title: R30N-3


title: R45N-2


title: 10X36_1_AAATGGGAAAGGGC


title: R15N-2


title: R10N-2


title: 10X36_1_AAATCTGAGTGTAC


title: R5N-2


title: 10X36_1_AAAGTTTGGAATGA


title: 10X36_1_AAATCTGAGGGCAA


title: R90N-1


title: R60N-1


title: 10X36_1_AAAGGCCTCGATAC


title: 10X36_1_AAAGTTTGAGGTCT


title: 10X36_1_AAAGGCCTCAAAGA


title: R45N-1


title: 10X36_1_AAAGCAGATTAGGC


title: R30N-1


title: 10X36_1_AAAGCAGACAGTTG


title: R20N-1


title: 10X36_1_AAAGATCTTCAGAC


title: R15N-1


title: 10X36_1_AAATGGGAAGCATC


title: 10X36_1_AAAGAGACAACGTC


title: 10X36_1_AAAGAGACCTAGTG


title: 10X36_1_AAACTTGATGGTGT


title: R5N-1


title: R120C-3


title: 10X36_1_AAACTTGAGAATCC


title: R60C-3


title: R45C-3


title: 10X36_1_AACACGTGCATTCT


title: 10X36_1_AAACTTGAACAGCT


title: R120N-1


title: 10X36_1_AAACGGCTTTTCTG


title: 10X36_1_AAACGGCTTCTCAT


title: R20C-3


title: R120N-2


title: 10X36_1_AAACGCACGTTTCT


title: 16_xf45_TAP

city: Mainz

first: Emil

last: Karaulanov

address: Ackermannweg 4

country: Germany

institute: Institute of Molecular Biology

description: gtsf-1(xf45) animals, sRNAs were treated with TAP before library prep

data_processing:
The raw sequence reads in FastQ format were cleaned from adapter sequences and size-selected in the range 18-30 bases (plus additional 8 random bases) using cutadapt v.1.2.1 (http://cutadapt.readthedocs.org) with parameters ‘-a AGATCGGAAGAGCACACGTCT -O 10 -m 26 -M 38’.
Mapping  to the Caenorhabditis elegans genome (Ensembl WBcel235/ce11 assembly) with concomitant trimming of the 8 random bases was performed using Bowtie v.1.1.2 (http://bowtie-bio.sourceforge.net) with parameters ‘-p 8 -v 1 -M 1 --best --strata --tryhard --chunkmbs 512 --trim5 4 --trim3 4 –S’. The resulting SAM alignment files were converted into sorted BAM files using Samtools v.1.3.1 (http://www.htslib.org).
Gene annotation in GTF format was downloaded from Ensembl (release 89). Structural reads were considered reads mapping sense to annotated rRNAs, tRNAs, snRNAs or snoRNAs, and these were removed from further analyses using Bedtools v.2.25.0 (http://bedtools.readthedocs.io).
22G and 26G small RNAs, i.e. 22 and 26 base reads starting with G, were extracted from the BAM files with Samtools and Awk. For browser visualisation, the reads were converted into reads-per-million (RPM) normalized bigWig coverage tracks using Bedtools and the UCSC utility bedGraphToBigWig (http://hgdownload.soe.ucsc.edu/admin/exe/linux.x86_64).
Genome_build: WBcel235 (ce11)
Supplementary_files_format_and_content: All mapped small RNA reads in BAM format (.bam files) and normalized coverage tracks of 22G or 26G small RNAs in bigWig format (.bw files).

organism: Caenorhabditis elegans

source_name: Gravid adult - whole animal

growth_protocol: Adult animals were bleached and their embryos were hatched overnight in M9 buffer. Synchronized L1 larvae were plated, grown for 63h until adulthood and egg laying onset.

extract_protocol:
Animals were collected and subsequently digested with Proteinase K. Total RNA was acquired using Trizol LS reagent, according to the manufacturer's instructions. Samples were enriched for sRNAs using a mirVana Kit (Life Technologies #AM1561). Each sRNA enriched sample was treated in three different ways: 1) directly cloned, 2) treated with Tobacco Acid Phosphatase (TAP), and 3) Oxidized. 1 µg of RNA was treated with 5 U of tobacco acid phosphatase (Epicenter) at 37°C for 2 h to digest 5’ tri- and di-phosphates to mono-phosphates. To oxidate sRNAs, 3 µg of sRNA enriched sample was incubated for 10 minutes at room temperature with 200 mM NaIO4, 5X borate buffer (148 mM Borax, 148 mM Boric acid and adjust pH to 8.6) and nuclease-free water to a total of 20 µL reaction volume. Then 2 µL of 100% glycerol were added to stop the reaction for 10 minutes at room temperature. Purified RNA was precipitated with 100% isopropanol and Glycoblue overnight at -20°C. The pellet was washed once with 75% ethanol and dissolved in nuclease-free water. Then, RNA was size-selected between 18- to 30-nt on 15% TBE-urea gel. Purified fraction was confirmed by Bioanalyzer sRNA chip (Agilent).
Small RNA library preparation was based on the NEBNext Multiplex sRNA Library Prep Set for Illumina (New England BioLabs) with slight modifications. To counteract ligation biases, the 3’ and 5' adapters contained 4 random bases at the 5’- and 3’-end, respectively, and were chemically synthesized by BioScientific. Adapter-ligated RNA was reverse-transcribed and PCR-amplified for 14 cycles using index primers. The PCR-amplified cDNA construct was purified using AMPure XP beads (Beckman Coulter). The purified PCR reaction was checked on the Bioanalyzer using High Sensitivity DNA chip (Agilent). Size selection of the sRNA library was done on LabChip XT instrument (Perkin Elmer) using DNA 300 assay kit. Only the fraction containing 140-165 bp was pooled in equal molar ratio. The resulting 10 nM pool was denatured to 10 pmol with 5% PhiX spike-in and sequenced as single-read on HiSeq 2500 (Illumina) in rapid mode for 51 cycles (plus 7 cycles index read)  using on-board cluster generation.

tag: strain

tag: genotype


title: 10X36_1_AAACATTGCTAGTG


title: R10N-1


title: 15_xf44

description: gtsf-1(xf44) animals, sRNAs were directly cloned


title: 14_xf44_oxid

description: gtsf-1(xf44) animals, sRNAs were oxidized before library prep


title: 10X36_1_AAACATACTCCGAA


title: R5C-3


title: R10C-3


title: 12_xf43

description: gtsf-1(xf43) animals, sRNAs were directly cloned


title: 10_xf43_TAP

description: gtsf-1(xf43) animals, sRNAs were treated with TAP before library prep


title: 11_xf43_oxid

description: gtsf-1(xf43) animals, sRNAs were oxidized before library prep


title: R120C-2


title: R90C-3


title: 09_N23

description: N2 biological replicate III, sRNAs were directly cloned

value: Wild-type


title: 18_xf45

description: gtsf-1(xf45) animals, sRNAs were directly cloned


title: R30C-3


title: R90C-2


title: 07_N23_TAP

description: N2 biological replicate III, sRNAs were treated with TAP before library prep


title: 17_xf45_oxid

description: gtsf-1(xf45) animals, sRNAs were oxidized before library prep


title: 06_N22

description: N2 biological replicate II, sRNAs were directly cloned


title: R60C-2


title: R45C-2


title: R15C-3


title: 05_N22_oxid

description: N2 biological replicate II, sRNAs were oxidized before library prep


title: 04_N22_TAP

description: N2 biological replicate II, sRNAs were treated with TAP before library prep


title: 03_N21

description: N2 biological replicate I, sRNAs were directly cloned


title: R30C-2


title: R20C-2


title: R10C-2


title: 13_xf44_TAP

description: gtsf-1(xf44) animals, sRNAs were treated with TAP before library prep


title: 02_N21_oxid

description: N2 biological replicate I, sRNAs were oxidized before library prep


title: 08_N23_oxid

description: N2 biological replicate III, sRNAs were oxidized before library prep


title: R5C-2


title: R90C-1


title: R60C-1


title: R120C-1


title: R0-3


title: R45C-1


title: R0-2


title: R20C-1


title: R30C-1


title: R10C-1


title: R5C-1


title: 01_N21_TAP

description: N2 biological replicate I, sRNAs were treated with TAP before library prep


title: S45N-3

source_name: Seedling shoot

value: Shoot


title: S20N-3


title: S30N-3


title: R0-1


title: S120N-2


title: S90N-2


title: S120N-3


title: R15C-2


title: S45N-2


title: S90N-3


title: S60N-3


title: S15N-3


title: S10N-3


title: S30N-2


title: S5N-3


title: Male_CTCFchip_Repl4_G100_M2

city: Boston

first: David

middle: J.

last: Waxman

state: MA

address: 5 Cummington Mall

department: Department of Biology and Bioinformatics Program

institute: Boston University

description: ChIP-seq Library

library_source: genomic

data_processing:
READ MAPPING: ChIP-seq reads were aligned using Bowtie2 (Langmead et al 2009, Genome Biology) with default settings. Reads were filtered so that only uniquely-aligned reads were used for downstream analysis.
PEAK CALLING: Peaks were called using Macs2 (Zhang et al 2008, Genome Biology) with default parameters without filtering for PCR duplicates. Peaks were filtered to remove blacklisted regions (www.sites.google.com/site/anshulkundaje/projects/blacklists) and also regions called as peaks that contain only PCR duplicated reads.
Genome_build: mm9
Supplementary_files_format_and_content: Replicate merged peak lists for CTCF (Male_CTCF_merged.bed) and cohesin/Rad21 (Male_Rad21_merged.bed). Samples were combined at the fastq level and processed through a standard ChIP-seq pipeline as described. An additional CTCF peak list is provided in relation to the main text, in which CTCF peaks are categorized as TAD/subTAD loop anchors, other cohesin-and-CTCF sites, or lone CTCF sites. This file also includes motif and directionality information.

molecule: genomic DNA

source_name: Liver

growth_protocol: Male and female CD1 mice, 8-9 weeks of age, were purchased from Charles River Laboratories (Crl:CD1(ICR)).

extract_protocol:
Nuclei were isolated by ultracentrifugation in a high sucrose buffer then crosslinked with 0.8% formaldehyde for 9 minutes. Samples were sonicated with a Bioruptor Twin (Diagenode, UCD-400) until the majority of DNA fragments were between 100-400bp (60-70 cycles total, 30 sec ON 30 sec OFF). Chromatin was quantified and 70 ug of chromatinized DNA was incubated with the indicated ChIP antibody.
Samples were prepared for sequencing with NEBNext Ultra II DNA Library Prep Kit for Illumina according to the manufacturer's directions for low input samples (New England Biolabs, #E7645). All samples were subjected to double-sided SPRI size selection prior to PCR amplification (Agencourt AMPure XP; Beckman Coulter: A63882). Samples were barcoded using New England Biolabs NEBNext Multiplex Oligos for Illumina (#E7335) with 8 rounds of PCR per the manufacturer's instructions.

tag: tissue

value: Liver

value: Crl:CD1(ICR) (Charles River, strain code #022)

tag: chip antibody

value: Millipore anti CTCF (cat# 07-779)

tag: Sex

value: Male

tag: age

value: 8 weeks


title: S60N-2


title: S5N-2


title: Male_Rad21chip_Repl3_G100_M5

description: ChIP-seq Library

value: Abcam anti Rad21 (cat# Ab992)


title: yAdu3

city: Heidelberg

first: Hellmut

middle: G

last: Augustin

address: Im Neuenheimer Feld 280

institute: DKFZ Heidelberg

data_processing:
Sequencing strategy: 100bp Paired-End
RTA 1.17.21.3
The sequenced reads were aligned to the mouse reference genome mm10 using STAR (Dobin et al. 2013) allowing up to 10 mismatches.
DEseq2 was used to test for differential gene expression.
Genome_build: mm10
Supplementary_files_format_and_content: RPKM_RNAseq_lungEC_inf_vs_yAdu.txt includes RPKM values for each Sample as calculated by DEseq2.

source_name: lung endothelial cells

extract_protocol:
Lung endothelial cells were isolated by FACS, and RNA was harvested using Arcturus PicoPure RNA Isolation Kit (Life Technologies).
Libraries were prepared with 10ng total RNA using the SMARTer Ultra Low RNA Kit for Illumina Sequencing (Clontech) according to manufacturer’s protocol

tag: stain

value: lung

value: endothelial cells

value: young adult


title: R15C-1


title: S60N-1


title: inf_1

data_processing:
Base caller: Illumina bcl2fastq v1.8.4
At each cytosine position, reads that maintain the cytosine status were considered methylated, and the reads that have cytosine converted to thymine were considered unmethylated. Only bases with Phred-scaled quality score of ≥20 were considered. First 9bp of the second read and last 9bp before the adapter in the first read were excluded from methylation calling.
We used a mapping method as described earlier (Johnson et al. 2012 Current protocols in molecular biology) modified for reads from tagmentation-based whole-genome bisulfite sequencing. Briefly, the mm10 reference genome was transformed in silico for both the top strand (C to T) and bottom strand (G to A). Before alignment, adaptor sequences were trimmed using SeqPrep (https://github.com/jstjohn/SeqPrep). Then the first read in each read pair was C-to-T converted and the 2nd read in the pair was G-to-A converted. The converted reads were aligned to a combined reference of the transformed top (C to T) and bottom (G to A) strands using BWA (bwa-0.6.1-tpx) (Li and Durbin 2009 Bioinformatics) with default parameters except the quality threshold for read trimming (-q) of 20 and the Smith-Waterman for the unmapped mate disabled (-s).
DMR calling was conducted on methylation data without smoothing (due to sufficiently high coverage) with the bsseq v1.60 package (Hansen et al. 2012 Genome Biology) for R statistical software v3.2.2. We applied a coverage filter that required a minimum coverage of 8x per CpG in at least two of the three samples per group. The DMR model comprised at least 3 consecutive significant CpGs (each p<0.05, t-test) and a minimum difference of 10% in mean methylation between groups.
Genome_build: mm10

extract_protocol:
Genomic DNA from sorted EC was isolated using the QIAamp DNA Micro Kit (Qiagen) according to the manufacturer’s instructions.
Library preparation was performed as described previously (Wang et al. 2013 Nature Protocols) using 30 ng genomic mouse DNA as input.

value: infant


title: inf_2


title: S20N-2


title: yAdu_3


title: S30N-1


title: inf3


title: yAdu_1


title: S15N-1


title: S120N-1


title: S10N-2


title: S90N-1


title: yAdu2


title: yAdu1


title: S45N-1


title: inf2


title: inf1


title: S10N-1

tag: treatment


title: S20N-1


title: R20N-2


title: S15C-3

value: KCl


title: S10C-3


title: S45C-2


title: S20C-2


title: S15N-2


title: yAdu_2


title: S5N-1


title: S10C-2


title: inf_3


title: S120C-1


title: S90C-3


title: S60C-3


title: S120C-3


title: S90C-2


title: S45C-3


title: S30C-3


title: S20C-3


title: S45C-1


title: S20C-1


title: S30C-1


title: S0-2


title: S0-3


title: Endogenous BRM Input rep 2

first: Neus

last: Visa

address: Svante Arrhenius väg 20c

department: Molecular Biosciences, The Wenner-Gren Institute (MBW)

institute: Stockholm University

description:
processed data file;
BRM_ChIP-seq_count_table.txt
Endogenous BRM Input  2

data_processing:
BRM RNA-Seq reads were aligned to the D. melanogaster reference genome dm3 by TopHat2 with at most 2 mismatches.
BRM ChIP-Seq reads were aligned to the D. melanogaster reference genome dm3 by Bowtie with at most 2 mismatches.
Reads that appeared more than twice at the same position on the same strand were discarded to remove PCR duplication.
ngs.plot software was used to calculate BRM enrichment and for metagene plotting.
Genome_build: dm3
Supplementary_files_format_and_content: For RNA-seq and ChIP-seq data: bam alignment files and raw read count per gene tables.

organism: Drosophila melanogaster

source_name: Endogenous BRM Input

growth_protocol: S2 cells were cultured at 28 °C according to the instructions of the Drosophila Expression System manual from Invitrogen. 3x106 S2 cells were cultured in 6-well plates overnight, washed with serum-free and antibiotic-free Schneider’s medium, and treated with 30 μg of dsRNA/well. The cells were harvested 48 hours after the addition of dsRNA. The expression of recombinant proteins was induced by copper sulfate during the final 24 hours of dsRNA treatment.

extract_protocol:
Input DNA was isolated with the ChIP cleanup and concentrator Kit (Zymo Research)
Input DNA and immunoprecipitated DNA samples were used for Rubicon Thruplex-FD library preparation and sequenced in a Illumina HiSeq2500 instrument

tag: cell line

value: S2 cells

value: Monoclonal against Drosophila BRM (non-commercial)


title: Endogenous BRM Input rep 1

description:
processed data file;
BRM_ChIP-seq_count_table.txt
Endogenous BRM Input  1


title: Endogenous BRM IP rep 1

description:
processed data file;
BRM_ChIP-seq_count_table.txt
Endogenous BRM IP  1

source_name: Endogenous BRM IP

extract_protocol:
Immunoprecipitated DNA was isolated with the ChIP cleanup and concentrator Kit (Zymo Research)
Input DNA and immunoprecipitated DNA samples were used for Rubicon Thruplex-FD library preparation and sequenced in a Illumina HiSeq2500 instrument


title: K804R dsBRM biological rep 2

description:
processed data file;
BRM_ChIP-seq_count_table.txt
K804R dsBRM  2

source_name: K804R dsBRM

extract_protocol:
RNA was extracted from cultured cells with TRIzol (Ambion).
Clustered using cBot and sequenced on HiSeq2500

treatment_protocol: S2 cells were treated with dsRNA to knock down BRM. Control cells were treated in parallel with dsRNA that was complementary to GFP

tag: plasmid

value: ATPase mutant BRM plasmid

tag: silencing

value: dsBRM silencing


title: S5C-3


title: S120C-2


title: S60C-2


title: K804R dsBRM biological rep 1

description:
processed data file;
BRM_RNA-seq_count_table.txt
K804R dsBRM  1


title: S15C-2


title: S5C-2


title: WT dsBRM biological rep 1

description:
processed data file;
BRM_RNA-seq_count_table.txt
WT dsBRM  1

source_name: WT dsBRM

value: Recombinant WT BRM plasmid


title: empty dsBRM biological rep 2

description:
processed data file;
BRM_RNA-seq_count_table.txt
empty dsBRM  2

source_name: empty dsBRM

value: empty plasmid


title: empty dsGFP biological rep 2

description:
processed data file;
BRM_RNA-seq_count_table.txt
empty dsGFP  2

source_name: empty dsGFP

value: dsGFP control


title: Male_CTCFchip_Repl2_G133_M13

description: ChIP-seq Library

value: Liver

value: Crl:CD1(ICR) (Charles River, strain code #022)


title: Male_CTCFchip_Repl3_G133_M14

description: ChIP-seq Library

value: Millipore anti CTCF (cat# 07-779)

value: Male


title: S90C-1

value: Shoot


title: S60C-1


title: S30C-2


title: S15C-1


title: S5C-1


title: Endogenous BRM IP rep 2

description:
processed data file;
BRM_ChIP-seq_count_table.txt
Endogenous BRM IP  2


title: Colorectal tissue Cancer 117

status: Public on May 10 2018

city: Rochester

first: Wilson

last: Wu

state: MN

address: 200 First Street SW

department: Gastroenterology and Hepatology

institute: Mayo Clinic

description: small RNA

data_processing:
Initial analysis was performed using the CAP-miRSeq pipeline (http://bioinformaticstools.mayo.edu/research/cap-mirseq/). Briefly, the reads were trimmed of the 3' NEB Next small RNA adapters with Cutadapt. Mature miRNA were quantified using mirdeep2 by aligning reads to the human mirbase v19 mature miRNA sequences.
Genome_build: hg19
Supplementary_files_format_and_content: Tab-delemited text file containing raw read counts for each human mirbase v19 mature miRNA.

organism: Homo sapiens

source_name: Colorectal tissue

extract_protocol:
Total RNA was extracted using RecoverAll Total Nucleic Acid Isolation Kit for FFPE (Thermo Fisher Scientific, MA, USA).
Five hundred nano-gram RNA per sample was used to prepare small RNA libraries using NEBNext® Small RNA Library Prep Set following manufacturer’s guide (New England Biolabs, MA, USA). Number of cycle for PCR enrichment was adjusted based on initial library amount quantified by qPCR. Up to 24 different libraries were indexed and pooled in a single sequencing lane in a randomized fashion. The libraries were sequenced at 50 bp single-read mode on an Illumina HiSeq 2000 sequencing system (Illumina, CA, USA).

tag: pathology

value: Cancer


title: Colorectal tissue Cancer 118

description: small RNA


title: Colorectal tissue Sessile Serrated Adenoma 116

description: small RNA

value: Sessile Serrated Adenoma


title: S10C-1


title: S0-1


title: Colorectal tissue Adenoma 115

description: small RNA

value: Adenoma


title: Colorectal tissue Cancer 119

description: small RNA


title: empty dsBRM biological rep 1

description:
processed data file;
BRM_RNA-seq_count_table.txt
empty dsBRM  1


title: Colorectal tissue Adenoma 112

description: small RNA


title: Colorectal tissue Cancer 110

description: small RNA


title: empty dsGFP biological rep 1

description:
processed data file;
BRM_RNA-seq_count_table.txt
empty dsGFP  1


title: Male_CTCFchip_Repl1_G133_M9

description: ChIP-seq Library

value: 8 weeks


title: WT dsBRM biological rep 2

description:
processed data file;
BRM_RNA-seq_count_table.txt
WT dsBRM  2


title: Colorectal tissue Cancer 107

description: small RNA


title: Male_Rad21chip_Repl2_G133_M6

description: ChIP-seq Library

value: Abcam anti Rad21 (cat# Ab992)


title: Colorectal tissue Cancer 105

description: small RNA


title: Colorectal tissue Adenoma 120

description: small RNA


title: Colorectal tissue Cancer 114

description: small RNA


title: Male_Rad21chip_Repl1_G133_M3

description: ChIP-seq Library


title: Colorectal tissue Cancer 101

description: small RNA


title: Colorectal tissue Cancer 113

description: small RNA


title: Colorectal tissue Adenoma 99

description: small RNA


title: Colorectal tissue Adenoma 108

description: small RNA


title: Colorectal tissue Cancer 94

description: small RNA


title: Colorectal tissue Cancer 98

description: small RNA


title: Colorectal tissue Adenoma 109

description: small RNA


title: Colorectal tissue Cancer 111

description: small RNA


title: Colorectal tissue Normal 96

description: small RNA

value: Normal


title: Colorectal tissue Cancer 106

description: small RNA


title: Colorectal tissue Cancer 92

description: small RNA


title: Colorectal tissue Adenoma 91

description: small RNA


title: Colorectal tissue Cancer 103

description: small RNA


title: Colorectal tissue Cancer 104

description: small RNA


title: Colorectal tissue Cancer 88

description: small RNA


title: Colorectal tissue Cancer 102

description: small RNA


title: Blood Normal 83

description: small RNA

source_name: Blood


title: Colorectal tissue Normal 84

description: small RNA


title: Colorectal tissue Adenoma 82

description: small RNA


title: Colorectal tissue Adenoma 81

description: small RNA


title: Colorectal tissue Cancer 97

description: small RNA


title: Colorectal tissue Cancer 80

description: small RNA


title: Colorectal tissue Cancer 95

description: small RNA


title: Colorectal tissue Adenoma 93

description: small RNA


title: Blood Normal 89

description: small RNA


title: Colorectal tissue Adenoma 87

description: small RNA


title: Blood Normal 74

description: small RNA


title: Blood Normal 72

description: small RNA


title: Colorectal tissue Adenoma 71

description: small RNA


title: Colorectal tissue Sessile Serrated Adenoma 86

description: small RNA


title: Colorectal tissue Adenoma 85

description: small RNA


title: Blood Normal 69

description: small RNA


title: Blood Normal 79

description: small RNA


title: Blood Normal 68

description: small RNA


title: Colorectal tissue Cancer 78

description: small RNA


title: Colorectal tissue Adenoma 77

description: small RNA


title: Blood Normal 65

description: small RNA


title: Colorectal tissue Normal 66

description: small RNA


title: Colorectal tissue Cancer 63

description: small RNA


title: Blood Normal 62

description: small RNA


title: Colorectal tissue Normal 61

description: small RNA


title: Colorectal tissue Cancer 76

description: small RNA


title: Blood Normal 58

description: small RNA


title: Colorectal tissue Cancer 90

description: small RNA


title: Colorectal tissue Cancer 73

description: small RNA


title: Blood Normal 56

description: small RNA


title: Blood Normal 57

description: small RNA


title: Colorectal tissue Normal 67

description: small RNA


title: Colorectal tissue Adenoma 70

description: small RNA


title: Colorectal tissue Normal 64

description: small RNA


title: Blood Normal 55

description: small RNA


title: Blood Normal 54

description: small RNA


title: Colorectal tissue Cancer 60

description: small RNA


title: Colorectal tissue Normal 52

description: small RNA


title: Colorectal tissue Cancer 59

description: small RNA


title: Colorectal tissue Sessile Serrated Adenoma 51

description: small RNA


title: Blood Normal 50

description: small RNA


title: Colorectal tissue Adenoma 100

description: small RNA


title: Blood Normal 49

description: small RNA


title: Colorectal tissue Normal 48

description: small RNA


title: Colorectal tissue Adenoma 46

description: small RNA


title: Blood Normal 44

description: small RNA


title: Blood Normal 47

description: small RNA


title: Colorectal tissue Normal 39

description: small RNA


title: Colorectal tissue Cancer 42

description: small RNA


title: Colorectal tissue Cancer 43

description: small RNA


title: Colorectal tissue Cancer 40

description: small RNA


title: Colorectal tissue Adenoma 37

description: small RNA


title: Blood Normal 41

description: small RNA


title: Colorectal tissue Adenoma 38

description: small RNA


title: Colorectal tissue Normal 36

description: small RNA


title: Colorectal tissue Sessile Serrated Adenoma 35

description: small RNA


title: Colorectal tissue Normal 33

description: small RNA


title: Colorectal tissue Cancer 34

description: small RNA


title: Blood Normal 32

description: small RNA


title: Colorectal tissue Sessile Serrated Adenoma 31

description: small RNA


title: Colorectal tissue Sessile Serrated Adenoma 29

description: small RNA


title: Colorectal tissue Adenoma 26

description: small RNA


title: Blood Normal 53

description: small RNA


title: Blood Normal 25

description: small RNA


title: Colorectal tissue Normal 30

description: small RNA


title: Blood Normal 28

description: small RNA


title: Colorectal tissue Normal 24

description: small RNA


title: Colorectal tissue Adenoma 45

description: small RNA


title: Colorectal tissue Normal 20

description: small RNA


title: Colorectal tissue Sessile Serrated Adenoma 21

description: small RNA


title: Colorectal tissue Adenoma 18

description: small RNA


title: Colorectal tissue Sessile Serrated Adenoma 22

description: small RNA


title: Blood Normal 27

description: small RNA


title: Blood Normal 17

description: small RNA


title: Colorectal tissue Normal 16

description: small RNA


title: Colorectal tissue Normal 13

description: small RNA


title: Colorectal tissue Sessile Serrated Adenoma 19

description: small RNA


title: Colorectal tissue Normal 11

description: small RNA


title: Colorectal tissue Adenoma 10

description: small RNA


title: Colorectal tissue Normal 9

description: small RNA


title: Colorectal tissue Cancer 14

description: small RNA


title: Colorectal tissue Adenoma 6

description: small RNA


title: Colorectal tissue Sessile Serrated Adenoma 8

description: small RNA


title: Colorectal tissue Sessile Serrated Adenoma 12

description: small RNA


title: Colorectal tissue Adenoma 5

description: small RNA


title: Colorectal tissue Cancer 4

description: small RNA


title: Colorectal tissue Normal 3

description: small RNA


title: Colorectal tissue Sessile Serrated Adenoma 1

description: small RNA


title: Colorectal tissue Sessile Serrated Adenoma 23

description: small RNA


title: Colorectal tissue Sessile Serrated Adenoma 2

description: small RNA


title: Colorectal tissue Adenoma 15

description: small RNA


title: Vian-2018-HIC059

city: bethesda

first: Seolkyoung

last: Jung

state: MD

address: 10 Center Dr

department: NIAMS

institute: NIH

data_processing:
The paired end reads were aligned separately using BWA against the mm9 mouse build or the b37 human build
PCR duplicates, low mapping quality and unligated reads were removed using Juicer (see Rao, Huntley, et al Cell 2014; Durand, Shamim, et al Cell Systems 2016)
Contact matrices were constructed at various resolutions and normalized using Juicer (see Rao, Huntley, et al Cell 2014; Durand, Shamim, et al Cell Systems 2016)
loops were annotated using HiCCUPS (see Rao, Huntley, et al Cell 2014; Durand, Shamim, et al Cell Systems 2016), domains were annotated using Arrowhead (see Rao, Huntley, et al Cell 2014; Durand, Shamim, et al Cell Systems 2016)
Genome_build: mm9 (mouse), b37 (human)
Supplementary_files_format_and_content: .hic file (contains contact matrices at various resolutions in an easy to visualize and access format) (see Durand, Robinson, et al Cell Systems 2016)

source_name: B-lymhoblastoids

extract_protocol:
Cells were crosslinked and then lysed with nuclei permeabilized but still intact. DNA was then restricted and the overhangs filled in incorporating a biotinylated base. Free ends were then ligated together in situ. Crosslinks were reversed, the DNA was sheared to 300-500bp and then biotinylated ligation junctions were recovered with streptavidin beads.
standard Illumina library construction protocol, Briefly, DNA was end-repaired using a combination of T4 DNA polymerase, E. coli DNA Pol I large fragment (Klenow polymerase) and T4 polynucleotide kinase. The blunt, phosphorylated ends were treated with Klenow fragment (32 to 52 exo minus) and dATP to yield a protruding 3- 'A' base for ligation of Illumina's adapters which have a single 'T' base overhang at the 3’ end. After adapter ligation DNA was PCR amplified with Illumina primers for 8-12 cycles and library fragments of 400-600 bp (insert plus adaptor and PCR primer sequences) were purified using SPRI beads. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the HiSeqX Ten, NextSeq 500, or HiSeq 2500 following the manufacturer's protocols

tag: protocol

value: in situ Hi-C


title: Vian-2018-HIC060


title: Vian-2018-HIC062


title: Vian-2018-HIC061


title: Vian-2018-HIC063


title: Vian-2018-HIC064


title: Vian-2018-HIC065


title: Colorectal tissue Normal 7

description: small RNA


title: Vian-2018-HIC058


title: Vian-2018-HIC054


title: Vian-2018-HIC056


title: Vian-2018-HIC049


title: Vian-2018-HIC053


title: Vian-2018-HIC043


title: Vian-2018-HIC055


title: Vian-2018-HIC045


title: Vian-2018-HIC057


title: Vian-2018-HIC052


title: Vian-2018-HIC050


title: Vian-2018-HIC051


title: K4KO_peritoneal macrophage 3

city: Cleveland

first: Mukesh

last: Jain

state: OH

address: 2103 Cornell Rd

institute: Case Western Reserve University

data_processing:
Software for the Illumina HiSeq 2500 was used for basecalling. 100bp paired-end sequencing performed
Reads from each sample were aligned to the mouse genome (UCSC mm10) using TopHat2 (Kim et al., 2013).
Macrophage gene expression was quantitated as fragments per kilobase of transcript per million mapped reads (FPKM) using Cufflinks (Trapnell et al., 2010). A total of 1,465 differentially expressed genes (DEGs) were identified and were defined as genes having FPKM>2, absolute log2 fold change >0.35, and an adjusted p-value cutoff q <0.05
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text file includes table of FPKM values for each sample

source_name: KO_peritoneal macrophage

growth_protocol: Mice were injected intraperitoneally with thioglycolate. 72 hours later, cells were isolated from the peritoneum. Cells were then plated in DMEM media containing 10% fetal bovine serum and 1% PenStrep + Glutamine. Cells were incubated for 24 hours at 5% CO2 and 37 degrees until harvesting cells for RNA.

extract_protocol:
RNA isolation was performed using the RNeasy kit (Qiagen, Hilden, Germany). cDNA sequencing libraries were prepared using the Illumina TruSeq Stranded Total RNA kit (poly A selection)
RNA libraries were prepared for sequencing using standard Illumina protocols

tag: strain background

tag: genotype/variation

value: myeloid-specific KLF4 knockout (K4KO)

tag: cell type

value: peritoneal macrophage


title: Vian-2018-HIC048


title: Vian-2018-HIC044


title: Vian-2018-HIC046


title: K4KO_peritoneal macrophage 1


title: WT_peritoneal macrophage 3

source_name: WT_peritoneal macrophage

value: lysozyme M Cre (wt)


title: WT_peritoneal macrophage 2


title: WT_peritoneal macrophage 1