title: UW_ChipSeq_AG04450_H3K27me3

tag: lab

value: UW

tag: lab description

value: Stamatoyannopoulous - University of Washington

value: ChipSeq

value: Chromatin IP Sequencing

value: fetal lung fibroblast

value: normal

tag: cell lineage

value: endoderm

value: M

tag: antibody

tag: antibody antibodydescription

value: rabbit polyclonal. Antibody Target:H3K27me3

tag: antibody targetdescription

value: Histone H3 (tri-methyl K27). Marks promoters that are silenced by Polycomb proteins in a given lineage; large domains are found at inactive developmental loci.

tag: antibody vendorname

value: Millipore

tag: antibody vendorid

tag: treatment description

tag: control


title: brain RNAseq Fus/Tls-oligo rep1.2

status: Public on Sep 30 2012

type: SRA

city: La Jolla

first: Gene

last: Yeo

state: CA

address: 2880 Torrey Pines Scenic Dr. Room 3805/Yeo Lab

country: USA

institute: UCSD

description: strand-specific RNAseq

library_source: transcriptomic

data_processing:
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm9 or hg18 whole genome using bowtie v0.12.2 with parameters -q -p 4 -e 100 -y -a -m 10 --best --strata
Genome_build: mm9
Supplementary_files_format_and_content: BED format of all reads

molecule: total RNA

organism: Mus musculus

source_name: Striatum

extract_protocol: Cells from whole brain of ten mice were pooled; cell suspension was distributed in 10 cm cell culture dishes and irradiated three times at 400 mJ/cm2 on ice with short breaks between each exposure. Irradiated brain cells were precipitated by brief centrifugation at 4oC, resuspended in PBS and distributed in ten 1.6 mL tubes, each containing cells corresponding to one mouse brain. Cells were disrupted on ice using PBS containing 0.1% SDS, 0.5% deoxycholate (Na), 0.5% NP-40, DNase treated and cleared from cell debris. Cell extracts were then incubated with protein G paramagnetic beads (Invitrogen) coupled to a custom-made mouse monoclonal anti-TDP-43 antibody (Ling, 2010) (40mg of antibody per 400 mL of beads per sample).  After 4hrs, beads were washed and associated RNAs were treated with three different concentrations (100U, 0.2U and 0.02U per sample) of micrococcal nuclease (MNase, Roche), while attached on the beads. Samples were then treated with alkaline phosphatase (NEB) and an RNA-linker (5¢P-UCGUAUGCCGUCUUCUGCUUG-Pmm, Thermo Scientific) was attached to the 3¢ end of RNAs. At this step we saved 40ul of each sample for immunoblot analysis before radiolabelling. The remaining samples were then labeled with P32-g-ATP using T4 polynucleotide kinase (NEB). Unlabelled and radiolabeled RNA-protein complexes were then released from the beads by incubating at 70oC in NuPAGE LDS sample buffer without reducing agent (Invitrogen). Samples were then migrated on 10% Bis-Tris NuPAGE gels using MES buffer (Invitrogen) and transferred to nitrocellulose membranes (0.45 mm pore size, Invitrogen). The membrane with unlabelled samples was subjected to immunobloting using a primary antibody rabbit anti-TDP-43 (Aviva system biology; Cat #ARP38942_T100; 1:2,000) (see below for the immunoblot protocol). The membrane with the radiolabelled samples was rinsed with PBS, put in a clean plastic wrap and exposed to an autoradiography film. Using the autoradiogram as a guide, membrane pieces containing complexes with higher molecular weight than the expected protein size were excised. Isolated membrane pieces were then incubated with 4mg/mL Proteinase K (Takara Bio Inc.) at 37oC for 20 min with shaking at 1200 rpm. The reaction was stopped with the addition of 7 M Urea and incubation at 37oC for 20 min with shaking at 1000 rpm. The released RNA were then extracted with phenol:chloroform.  To precipitate the RNA, the aqueous phase was transferred in a separate tube where sodium acetate, linear acrylamide, ethanol and isopropanol were added and the samples were incubated at -20oC overnight. The next day, samples were centrifuged for 10 mins at maximum speed in a microcentrifuge at 4oC. RNA pellets were washed twice with 75% ethanol and dried at room temperature. RNAs were resuspended in an RNA ligation buffer and an RNA-linker (Bi-AAUGAUACGGCGACCACCGA, Thermo Scientific) was attached to their 5¢ end. Samples were treated once more with DNase I and precipitated with Phenol Chloroform as described above. RNAs were then reverse transcribed using SuperScript III (Invitrogen) and amplified by PCR using high fidelity Phusion polymerase (NEB) with the following primers: CAAGCAGAAGACGGCATACGA (P3) and AATGATACGACCACCGA (P5). Amplified libraries were then migrated in a 12% acrylamide gel and 75-200bp DNA fragments were excised and frozen at -20oC overnight. Acrylamide pieces were crushed into smaller pieces and DNA fragments were eluted by incubating overnight at 4oC in 50mM NaCl, 10mM TrisHCl, pH=8.0. Supernatants containing the DNA libraries were incubated with 3M sodium acetate and 100% ethanol with glycogen to facilitate DNA precipitation overnight at -80oC. DNA fragments were precipitated and pellets were resuspended in water and filtered through Spin X Centrifuge tube filter (Costar) to remove acrylamide contaminants. DNA libraries were precipitated and then resuspended in 12-20mL of DEPC-treated water. Libraries were quantified by the Qubit Quantitation Platform (Invitrogen) before loading on an Illumina Genome Analyzer 2 (GA2) flow-cell in a concentration of 8 pM per lane. Sequencing was carried out on the Illumina GA2 according to the manufacturer’s instructions for 36 cycles using the “P5” primer.

tag: tissue

value: brain striatum

tag: genetic background

tag: age

value: 8-10 weeks

tag: treatment

value: Fus/Tls anti-sense oligo


title: brain RNAseq control-oligo rep1.1

description: strand-specific RNAseq

value: control anti-sense oligo


title: UW_ChipSeq_AG04450_H3K27ac

status: Public on Sep 28 2012

city: Stanford

first: ENCODE

last: DCC

address: 300 Pasteur Dr

institute: ENCODE DCC

library_source: genomic

molecule: genomic DNA

organism: Homo sapiens

extract_protocol: Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeUwHistone

tag: datatype

tag: datatype description

tag: cell

tag: cell organism

value: human

tag: cell description

tag: cell karyotype

tag: cell sex

value: rabbit polyclonal. Antibody Target:H3K27ac

value: Histone H3 (acetyl K27). As with H3K9ac, associated with transcriptional initiation and open chromatin structure. It remains unknown whether acetylation has can have different consequences depending on the specific lysine residue targeted. In general, though, there appears to be high redundancy. Histone acetylation is notable for susceptibility to small molecules and drugs that target histone deacetylases.

value: abcam

value: No special treatment or protocol applies

value: std

tag: control description

value: Standard input signal for most experiments.

tag: controlid

tag: labexpid

tag: labversion

tag: replicate


title: HudsonAlpha_ChipSeq_K562_CEBPD_(SC-636)_v042211.1

extract_protocol: Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

value: ChipSeq

value: Chromatin IP Sequencing

tag: antibody antibodydescription

value: Rabbit polyclonal IgG, epitope mapping at the C-terminus of CEBPD of human origin. Antibody Target:CEBPD

tag: antibody targetdescription

value: The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related protein CEBP-alpha. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses, and may be involved in the regulation of genes associated with activation and/or differentiation of macrophages. The cytogenetic location of this locus has been reported as both 8p11 and 8q11 (provided by RefSeq).

tag: antibody vendorname

value: Santa Cruz Biotechnology

tag: antibody vendorid

tag: softwareversion

value: MACS

value: F

tag: antibody

tag: treatment description

tag: protocol

tag: protocol description

value: Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)


title: HudsonAlpha_ChipSeq_HCT-116_SP1_v042211.1

tag: datatype

tag: datatype description

value: Sp1(E-3) is a mouse monoclonal antibody raised against amino acids 121-345 mapping near the N-terminus of Sp1 ofhuman origin. Recommended for detection of Sp1 of m, r and h origin by WB, IP, IF and ELISA. Antibody Target:SP1

value: SP1 is a transcription factor that binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. It can interact with G/C-rich motifs from the serotonin receptor promoter.

value: M

tag: treatment


title: HudsonAlpha_ChipSeq_HCT-116_Max_v042211.1

value: Rabbit polyclonal IgG, epitope mapping at the C-terminus of Max of human origin. Antibody Target:Max

value: The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Multiple alternatively spliced transcript variants have been described for this gene but the full-length nature for some of them is unknown (RefSeq).

tag: cell sex

value: Max


title: HudsonAlpha_ChipSeq_SK-N-SH_JunD_v042211.1

source_name: SK-N-SH

value: rabbit polyclonal IgG. Antibody Target:JunD

value: The protein encoded by this intronless gene is a member of the JUN family, and a functional component of the AP1 transcription factor complex. It has been proposed to protect cells from p53-dependent senescence and apoptosis. Alternate translation initiation site usage results in the production of different isoforms. (provided by RefSeq)

tag: controlid

value: JunD

value: No special treatment or protocol applies


title: HudsonAlpha_ChipSeq_SK-N-SH_TCF12_v042211.1

value: ChipSeq

value: Chromatin IP Sequencing

value: HEB (A-20) Rabbit polyclonal IgG, 200 ?g/ml. Epitope mapping within the C-terminus of HEB of human origin. Antibody Target:TCF12

value: The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

tag: labexpid


title: HudsonAlpha_ChipSeq_HCT-116_CTCF_(SC-5916)_v042211.1

value: Goat polyclonal IgG, epitope mapping at the N-terminus of CTCF of human origin. Antibody Target:CTCF

value: This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. (provided by RefSeq)

value: Santa Cruz Biotechnology

value: MACS


title: HudsonAlpha_ChipSeq_SK-N-SH_YY1_(SC-281)_v042211.1

value: Rabbit polyclonal IgG, epitope mapping at the C-terminus of YY1 of human origin. Antibody Target:YY1

value: YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1

value: F

value: Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)


title: UNC_FaireSeq_MRT_A204

extract_protocol: Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeOpenChromFaire

value: FaireSeq

value: FAIRE-seq Open Chromatin

tag: labversion

value: baseAlignCounts.pl v 1,fseq v 1.84, iff_generic_female,p-value cutoff:0.1

tag: cell

tag: cell organism

value: human

tag: cell description

value: Malignant rhabdoid tumor (MRT) line, rhabdomyosarcoma

tag: cell karyotype

value: cancer

tag: cell lineage

value: missing


title: HudsonAlpha_ChipSeq_HCT-116_Sin3Ak-20_v042211.1

value: ChipSeq

value: Chromatin IP Sequencing

tag: antibody antibodydescription

value: Rabbit polyclonal, Sin3A (k-20). Antibody Target:SIN3AK20

tag: antibody targetdescription

value: Co-repressor interacting with HDAC1, N-coR, SMRT, and MeCP2

tag: antibody vendorname

tag: antibody vendorid

tag: softwareversion

value: M

value: No special treatment or protocol applies

tag: replicate


title: HudsonAlpha_ChipSeq_H1-hESC_Max_v042211.1

growth_protocol: missing

tag: datatype

tag: datatype description

value: Rabbit polyclonal IgG, epitope mapping at the C-terminus of Max of human origin. Antibody Target:Max

value: The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Multiple alternatively spliced transcript variants have been described for this gene but the full-length nature for some of them is unknown (RefSeq).

value: Santa Cruz Biotechnology

value: MACS

tag: antibody

value: Max

tag: treatment description

tag: protocol

tag: protocol description


title: UNC_FaireSeq_MCF-7_vehicle

treatment_protocol: vehicle

value: FaireSeq

value: FAIRE-seq Open Chromatin

tag: controlid

tag: labversion

value: baseAlignCounts.pl v 1,fseq v 1.84, iff_MCF7,p-value cutoff:0.1

tag: cell

value: mammary gland, adenocarcinoma. (PMID:4357757), newly promoted to tier 2:not in 2011 analysis

value: ectoderm

tag: cell sex

value: F

tag: treatment

value: vehicle

value: Charcoal stripped hormone-free FBS for 72 hours (Crawford)


title: HudsonAlpha_ChipSeq_K562_RevXlinkChromatin_v042211.1

value: ChipSeq

value: Chromatin IP Sequencing

tag: labexpid

tag: cell organism

value: human

tag: cell description

value: leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC

tag: cell karyotype

value: cancer

tag: cell lineage

value: mesoderm

value: RevXlinkChromatin

value: Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)


title: HudsonAlpha_ChipSeq_GM12878_RUNX3_(SC-101553)_v042211.1

tag: antibody antibodydescription

value: Mouse monoclonal IgG, raised against amino acids 191-300 of recombinant RUNX3 of human origin. Antibody Target:RUNX3

tag: antibody targetdescription

value: This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Multiple transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq)

tag: antibody vendorname

value: Santa Cruz Biotechnology

tag: antibody vendorid

tag: softwareversion

value: MACS

value: No special treatment or protocol applies

tag: replicate


title: HudsonAlpha_ChipSeq_K562_TEAD4_(SC-101184)_v042211.1

tag: datatype

tag: datatype description

value: Mouse monoclonal IgG, raised against recombinant protein TEF-3 (TEAD4) of human origin. Antibody Target:TEAD4

value: Member of the transcriptional enhancer factor (TEF) family of transcription factors, which contain the TEA/ATTS DNA-binding domain. It is preferentially expressed in the skeletal muscle, and binds to the M-CAT regulatory element found in promoters of muscle-specific genes to direct their gene expression.

tag: antibody

tag: treatment description

tag: protocol

tag: protocol description


title: HudsonAlpha_ChipSeq_H1-hESC_CREB1_(SC-240)_v042211.1

value: Mouse monoclonal IgG1; raised against amino acids 254-327 mapping within the DNA binding and dimerization domain of human CREB-1. Antibody Target:CREB1

value: This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. (provided by RefSeq)

tag: controlid

tag: labexpid

tag: cell sex

value: M

tag: treatment


title: HudsonAlpha_ChipSeq_MCF-7_JunD_v042211.1

value: ChipSeq

value: Chromatin IP Sequencing

value: rabbit polyclonal IgG. Antibody Target:JunD

value: The protein encoded by this intronless gene is a member of the JUN family, and a functional component of the AP1 transcription factor complex. It has been proposed to protect cells from p53-dependent senescence and apoptosis. Alternate translation initiation site usage results in the production of different isoforms. (provided by RefSeq)

value: F

value: JunD


title: HudsonAlpha_ChipSeq_MCF-7_NRSF_v042211.1

value: Mouse monoclonal. Antibody Target:NRSF

value: Neuron-restrictive silencer transcription factor

value: Dr. David Anderson at Caltech

value: (Made by Protein Expression Center:pec@caltech.edu)

value: NRSF

value: David Anderson, Caltech

value: Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)


title: HudsonAlpha_ChipSeq_T-47D_RevXlinkChromatin_v042211.1

tag: cell

tag: cell organism

value: human

tag: cell description

value: epithelial cell line derived from a mammary ductal carcinoma.

tag: cell karyotype

value: cancer

value: RevXlinkChromatin

value: No special treatment or protocol applies


title: HudsonAlpha_ChipSeq_ECC-1_USF-1_v042211.1

value: ChipSeq

value: Chromatin IP Sequencing

tag: antibody antibodydescription

value: Rabbit polyclonal, USF-1 (C-20). Antibody Target:USF1

tag: antibody targetdescription

value: The ubiquitously expressed cellular upstream stimulatory factor (USF) consists of USF-1 and USF-2 polypeptides which independently exhibit site-specific DNA binding and are members of the c-Myc-related family of regulatory factors containing helix-loop-helix domains. USF also contains a leucine repeat that is required for efficient DNA binding. USF was originally identified as an up- stream stimulatory factor that binds the core sequence CACGTG in the adeno- virus late promoter. These findings, together with the demonstration of coop- erative interaction between USF and the initiator-binding protein, TFII-I, raises the possibility of a more general involvement of USF in transcriptional regula- tion. While expression of both USF-1 and USF-2 species is ubiquitous, different ratios of USF homo- and heterodimers are found in different cell types.

tag: antibody vendorname

value: Santa Cruz Biotechnology

tag: antibody vendorid

value: MACS


title: HudsonAlpha_ChipSeq_SK-N-SH_Pbx3_v042211.1

tag: datatype

tag: datatype description

value: Rabbit Polyclonal IgG. Antibody Target:PBX3

tag: softwareversion

value: F

tag: replicate


title: HudsonAlpha_ChipSeq_ECC-1_Egr-1_v042211.1

value: Rabbit polyclonal, EGR-1 (588). Antibody Target:EGR1

value: Egr-1, Egr-2, Egr-3 and Egr-4 are nuclear transcription factors belonging to the Egr C2H2-type zinc-finger protein family and containing three C2H2-type zinc fingers. As immediate early proteins, Egr transcription factors are rapidly induced by diverse extracellular stimuli. Egr proteins are subject to tight differential control through diverse mechanisms at several levels of regulation including transcriptional, translational and post-translational (including glyco- sylation, phosphorylation and redox) mechanisms and protein-protein inter- action. Egr-1 binds to the DNA sequence 5'-CGCCCCCGC-3' (EGR-site), there- by activating transcription of target genes whose products are required for mitogenisis and differentiation. Egr-2 binds specific DNA sites located in the promoter region of HoxA4. Egr-2 defects cause congenital hypomyelination neuropathy (also designated Charcot-Marie-tooth disease) and Dejerine- Sottas neuropathology (also designated hereditary motor and sensory neuro- pathy III. Egr-3 is involved in muscle spindle development and is expressed in T cells 20 minutes following activation. EGR-4 binds to the EGR consensus motif GCGTGGGCG, functions as a transcriptional repressor, and displays autoregulatory activities, downregulating its on gene promoter in a dose dependent manner.

tag: antibody

tag: treatment description

tag: protocol

tag: protocol description


title: HudsonAlpha_ChipSeq_ECC-1_TEAD4_(SC-101184)_v042211.1

value: Mouse monoclonal IgG, raised against recombinant protein TEF-3 (TEAD4) of human origin. Antibody Target:TEAD4

value: Member of the transcriptional enhancer factor (TEF) family of transcription factors, which contain the TEA/ATTS DNA-binding domain. It is preferentially expressed in the skeletal muscle, and binds to the M-CAT regulatory element found in promoters of muscle-specific genes to direct their gene expression.

tag: controlid

tag: labexpid

tag: cell sex

tag: treatment


title: HudsonAlpha_ChipSeq_A549_Sin3Ak-20_EtOH_0.02pct_v042211.1

value: Rabbit polyclonal, Sin3A (k-20). Antibody Target:SIN3AK20

value: Co-repressor interacting with HDAC1, N-coR, SMRT, and MeCP2

value: M

value: 1 h with 0.02% Ethanol (Myers)

value: Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)


title: HudsonAlpha_ChipSeq_K562_CREB1_(SC-240)_v042211.1

value: ChipSeq

value: Mouse monoclonal IgG1; raised against amino acids 254-327 mapping within the DNA binding and dimerization domain of human CREB-1. Antibody Target:CREB1

value: This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. (provided by RefSeq)

value: No special treatment or protocol applies


title: HudsonAlpha_ChipSeq_SK-N-SH_RevXlinkChromatin_v042211.1

value: Chromatin IP Sequencing

tag: cell

value: SK-N-SH

tag: cell organism

value: human

tag: cell description

value: neuroblastoma, newly promoted to tier 2:not in 2011 analysis, the SK-N-SH line was established from a neuroblastoma in a 4 year old individual.

tag: cell karyotype

value: cancer

tag: cell lineage

value: ectoderm

value: RevXlinkChromatin


title: HudsonAlpha_ChipSeq_GM12878_NFIC_(SC-81335)_v042211.1

tag: datatype

tag: antibody antibodydescription

value: Mouse monoclonal IgG, raised against a recombinant protein corresponding to an internal region of NF-1 of human origin. Antibody Target:NFIC

tag: antibody targetdescription

value: Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

tag: antibody vendorname

value: Santa Cruz Biotechnology

tag: antibody vendorid

value: MACS

value: F


title: HudsonAlpha_ChipSeq_SK-N-SH_NFIC_(SC-81335)_v042211.1

tag: datatype description

tag: softwareversion

tag: antibody

tag: treatment description

value: Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)

tag: replicate


title: HudsonAlpha_ChipSeq_A549_SIX5_EtOH_0.02pct_v042211.1

value: ChipSeq

value: Six5 (K-20) Goat polyclonal IgG, 200?g/ml. Epitope mapping near the C-terminus of Six5 of human origin. Recommended for detection of Six5 of human origin by WB, IF and ELISA. Antibody Target:SIX5

value: The protein encoded by this gene is a homeodomain-containing transcription factor that appears to function in the regulation of organogenesis. This gene is located downstream of the dystrophia myotonica-protein kinase gene. Mutations in this geneare a cause of branchiootorenal syndrome type 2.

tag: controlid

tag: labexpid

tag: cell sex

value: M

value: 1 h with 0.02% Ethanol (Myers)

tag: protocol

tag: protocol description


title: HudsonAlpha_ChipSeq_K562_STAT5A_(SC-74442)_v042211.1

value: Chromatin IP Sequencing

value: Mouse monoclonal IgG, recommended for the detection of Stat5a and Stat5b of human origin by WB, IP, IF and ELISA. Antibody Target:STAT5A

value: The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. (provided by RefSeq)

tag: treatment

value: No special treatment or protocol applies


title: HudsonAlpha_ChipSeq_HL-60_RevXlinkChromatin_v042211.1

tag: cell

tag: cell organism

value: human

tag: cell description

value: promyelocytic leukemia cells, (PMID:276884)

tag: cell karyotype

value: cancer

tag: cell lineage

value: mesoderm


title: HudsonAlpha_ChipSeq_A549_CEBPB_(SC-150)_v042211.1

tag: datatype

tag: antibody antibodydescription

value: Rabbit polyclonal IgG, epitope mapping at the C-terminus of CEBPB of rat origin. Antibody Target:CEBPB

tag: antibody targetdescription

value: The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene (provided by RefSeq).

tag: antibody vendorname

value: Santa Cruz Biotechnology

tag: antibody vendorid


title: HudsonAlpha_ChipSeq_HepG2_TEAD4_(SC-101184)_v042211.1

tag: datatype description

value: Mouse monoclonal IgG, raised against recombinant protein TEF-3 (TEAD4) of human origin. Antibody Target:TEAD4

value: Member of the transcriptional enhancer factor (TEF) family of transcription factors, which contain the TEA/ATTS DNA-binding domain. It is preferentially expressed in the skeletal muscle, and binds to the M-CAT regulatory element found in promoters of muscle-specific genes to direct their gene expression.

tag: softwareversion

value: MACS

tag: antibody

tag: treatment description

value: Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)

tag: replicate


title: HudsonAlpha_ChipSeq_ECC-1_RevXlinkChromatin_v042211.1

value: ChipSeq

tag: controlid

tag: labexpid

value: epithelial cell line derived from an endometrium adenocarcinoma, fetal membranes were collected from women who underwent planned cesarean delivery at term, before labor and without rupture of membranes

value: endoderm

tag: cell sex

value: F

value: RevXlinkChromatin

tag: protocol

tag: protocol description


title: HudsonAlpha_ChipSeq_HCT-116_NRSF_v042211.1

value: Chromatin IP Sequencing

value: Mouse monoclonal. Antibody Target:NRSF

value: Neuron-restrictive silencer transcription factor

value: Dr. David Anderson at Caltech

value: (Made by Protein Expression Center:pec@caltech.edu)

value: M

value: NRSF

value: David Anderson, Caltech

tag: treatment

value: No special treatment or protocol applies


title: HudsonAlpha_ChipSeq_H1-neurons_RevXlinkChromatin_v042211.1

tag: cell

tag: cell organism

value: human

tag: cell description

value: neurons derived from H1 embryonic stem cells, newly promoted to tier 2:not in 2011 analysis

tag: cell karyotype

value: normal

tag: cell lineage

value: ectoderm


title: HudsonAlpha_ChipSeq_HepG2_MYBL2_(SC-81192)_v042211.1

tag: datatype

value: ChipSeq

tag: antibody antibodydescription

value: Mouse monoclonal IgG, raised against a recombinant protein corresponding to an internal region of B-Myb of human origin. Antibody Target:MYBL2

tag: antibody targetdescription

value: The protein encoded by this gene, a member of the MYB family of transcription factor genes, is a nuclear protein involved in cell cycle progression. The encoded protein is phosphorylated by cyclin A/cyclin-dependent kinase 2 during the S-phase of the cell cycle and possesses both activator and repressor activities. It has been shown to activate the cell division cycle 2, cyclin D1, and insulin-like growth factor-binding protein 5 genes. Transcript variants may exist for this gene, but their full-length natures have not been determined. (provided by RefSeq)

tag: antibody vendorname

value: Santa Cruz Biotechnology

tag: antibody vendorid

value: MACS

value: Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)


title: HudsonAlpha_ChipSeq_MCF-7_CEBPB_(SC-150)_v042211.1

tag: datatype description

value: Chromatin IP Sequencing

value: Rabbit polyclonal IgG, epitope mapping at the C-terminus of CEBPB of rat origin. Antibody Target:CEBPB

value: The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene (provided by RefSeq).

tag: softwareversion

value: F

tag: antibody

tag: treatment description

tag: replicate


title: HudsonAlpha_ChipSeq_GM12878_RevXlinkChromatin_v042211.1

tag: controlid

tag: labexpid

value: B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

value: mesoderm

tag: cell sex

value: RevXlinkChromatin

tag: protocol

tag: protocol description


title: HudsonAlpha_ChipSeq_HCT-116_RevXlinkChromatin_v042211.1

tag: cell

tag: cell organism

value: human

tag: cell description

value: colorectal carcinoma (PMID:7214343)

tag: cell karyotype

value: cancer

value: endoderm

value: M

tag: treatment

value: No special treatment or protocol applies


title: HudsonAlpha_ChipSeq_A549_TEAD4_(SC-101184)_v042211.1

tag: datatype

value: ChipSeq

tag: antibody antibodydescription

value: Mouse monoclonal IgG, raised against recombinant protein TEF-3 (TEAD4) of human origin. Antibody Target:TEAD4

tag: antibody targetdescription

value: Member of the transcriptional enhancer factor (TEF) family of transcription factors, which contain the TEA/ATTS DNA-binding domain. It is preferentially expressed in the skeletal muscle, and binds to the M-CAT regulatory element found in promoters of muscle-specific genes to direct their gene expression.

tag: antibody vendorname


title: HudsonAlpha_ChipSeq_MCF-7_TEAD4_(SC-101184)_v042211.1

tag: datatype description

value: Santa Cruz Biotechnology

tag: antibody vendorid

tag: softwareversion

value: MACS

tag: antibody

value: Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)


title: HudsonAlpha_ChipSeq_MCF-7_TCF12_v042211.1

value: Chromatin IP Sequencing

value: HEB (A-20) Rabbit polyclonal IgG, 200 ?g/ml. Epitope mapping within the C-terminus of HEB of human origin. Antibody Target:TCF12

value: The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

tag: controlid

tag: labexpid

tag: treatment description

tag: replicate


title: HudsonAlpha_ChipSeq_MCF-7_Sin3Ak-20_v042211.1

value: Rabbit polyclonal, Sin3A (k-20). Antibody Target:SIN3AK20

value: Co-repressor interacting with HDAC1, N-coR, SMRT, and MeCP2

tag: cell sex

value: F

tag: protocol

tag: protocol description


title: HudsonAlpha_ChipSeq_MCF-7_Max_v042211.1

value: Rabbit polyclonal IgG, epitope mapping at the C-terminus of Max of human origin. Antibody Target:Max

value: The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Multiple alternatively spliced transcript variants have been described for this gene but the full-length nature for some of them is unknown (RefSeq).

value: Max


title: HudsonAlpha_ChipSeq_PANC-1_NRSF_v042211.1

value: Mouse monoclonal. Antibody Target:NRSF

value: Neuron-restrictive silencer transcription factor

value: Dr. David Anderson at Caltech

value: (Made by Protein Expression Center:pec@caltech.edu)

value: M

value: NRSF

value: David Anderson, Caltech


title: HudsonAlpha_ChipSeq_ECC-1_CREB1_(SC-240)_v042211.1

value: ChipSeq

value: Mouse monoclonal IgG1; raised against amino acids 254-327 mapping within the DNA binding and dimerization domain of human CREB-1. Antibody Target:CREB1

value: This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. (provided by RefSeq)

value: Santa Cruz Biotechnology

value: MACS

value: No special treatment or protocol applies

value: Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)


title: HudsonAlpha_ChipSeq_K562_CEBPB_(SC-150)_v042211.1

value: Chromatin IP Sequencing

value: Rabbit polyclonal IgG, epitope mapping at the C-terminus of CEBPB of rat origin. Antibody Target:CEBPB

value: The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene (provided by RefSeq).

value: F


title: HudsonAlpha_ChipSeq_ECC-1_NFIC_(SC-81335)_v042211.1

value: Mouse monoclonal IgG, raised against a recombinant protein corresponding to an internal region of NF-1 of human origin. Antibody Target:NFIC

value: Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.


title: HudsonAlpha_ChipSeq_MCF-7_RevXlinkChromatin_v042211.1

tag: cell

tag: cell organism

value: human

tag: cell description

value: mammary gland, adenocarcinoma. (PMID:4357757), newly promoted to tier 2:not in 2011 analysis

tag: cell karyotype

value: cancer

tag: cell lineage

value: ectoderm

value: RevXlinkChromatin


title: HudsonAlpha_ChipSeq_PANC-1_RevXlinkChromatin_v042211.1

tag: datatype

value: ChipSeq

value: pancreatic carcinoma, (PMID:1140870) PANC-1 was established from a pancreatic carcinoma, which was extracted via pancreatico-duodenectomy specimen from a 56-year-old Caucasian individual. Malignancy of this cell line was verified via in vitro and in vivo assays.

value: endoderm

value: M

tag: treatment


title: HudsonAlpha_ChipSeq_HCT-116_CEBPB_(SC-150)_v042211.1

tag: datatype description

tag: antibody antibodydescription

tag: antibody targetdescription

tag: antibody vendorname

value: Santa Cruz Biotechnology

tag: antibody vendorid

value: MACS

value: The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions.  It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma.  The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes.  In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene (provided by RefSeq).

value: No special treatment or protocol applies


title: HudsonAlpha_ChipSeq_SK-N-SH_TEAD4_(SC-101184)_v042211.1

value: Chromatin IP Sequencing

value: Mouse monoclonal IgG, raised against recombinant protein TEF-3 (TEAD4) of human origin. Antibody Target:TEAD4

value: Member of the transcriptional enhancer factor (TEF) family of transcription factors, which contain the TEA/ATTS DNA-binding domain. It is preferentially expressed in the skeletal muscle, and binds to the M-CAT regulatory element found in promoters of muscle-specific genes to direct their gene expression.

tag: controlid

tag: labexpid

tag: softwareversion

tag: antibody

tag: treatment description

value: Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)


title: HudsonAlpha_ChipSeq_ECC-1_FOXM1_(SC-502)_v042211.1

value: Rabbit polyclonal IgG, epitope mapping at the C-terminus of FOXM1 of human origin. Antibody Target:FOXM1

value: The protein encoded by this gene is a transcriptional activator involved in cell proliferation. The encoded protein is phosphorylated in M phase and regulates the expression of several cell cycle genes, such as cyclin B1 and cyclin D1. Several transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq, Jul 2011)

tag: cell sex

value: F

tag: protocol

tag: protocol description

tag: replicate


title: HudsonAlpha_ChipSeq_HCT-116_SRF_v042211.1

value: Rabbit polyclonal. Antibody Target:SRF

value: Serum response transcription factor

value: SRF


title: HudsonAlpha_ChipSeq_MCF-7_GABP_v042211.1

value: ChipSeq

value: Mouse monoclonal, GABPa(G-1), IgG1. Antibody Target:GABP

value: The transcription factor GA-binding protein (GABP) is composed of two subunits, alpha and beta. Alpha binds to a specific DNA sequence.

value: GABP


title: HudsonAlpha_ChipSeq_HepG2_Max_v042211.1

value: Rabbit polyclonal IgG, epitope mapping at the C-terminus of Max of human origin. Antibody Target:Max

value: The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Multiple alternatively spliced transcript variants have been described for this gene but the full-length nature for some of them is unknown (RefSeq).

value: Santa Cruz Biotechnology

value: MACS

value: M

value: Max


title: HudsonAlpha_ChipSeq_A549_RevXlinkChromatin_v042211.1

value: Chromatin IP Sequencing

tag: cell

tag: cell organism

value: human

tag: cell description

value: epithelial cell line derived from a lung carcinoma tissue. (PMID:175022), "This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old caucasian male." - ATCC, newly promoted to tier 2:not in 2011 analysis

tag: cell karyotype

value: cancer

tag: cell lineage

value: endoderm

value: RevXlinkChromatin

value: No special treatment or protocol applies

value: Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)


title: HudsonAlpha_ChipSeq_GM12878_CEBPB_(SC-150)_v042211.1

tag: datatype

value: ChipSeq

tag: datatype description

tag: antibody antibodydescription

value: Rabbit polyclonal IgG, epitope mapping at the C-terminus of CEBPB of rat origin. Antibody Target:CEBPB

tag: antibody targetdescription

value: The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene (provided by RefSeq).

value: F

tag: treatment


title: HudsonAlpha_ChipSeq_HCT-116_Rad21_v042211.1

value: Rabbit polyclonal. Antibody Target:RAD21

value: Synthetic peptide (Human) conjugated to KLH - which represents a portion of human Rad21 encoded within exon 14

tag: antibody vendorname

value: abcam

tag: antibody vendorid

tag: controlid

tag: labexpid

tag: softwareversion


title: HudsonAlpha_ChipSeq_K562_TRIM28_(SC-81411)_v042211.1

value: Mouse monoclonal IgG, raised against a recombinant protein corresponding to an internal region of TRIM28 of human origin. Antibody Target:TRIM28

value: The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. (provided by RefSeq)

value: Santa Cruz Biotechnology

value: MACS

tag: cell sex

tag: antibody

tag: treatment description

tag: protocol

tag: protocol description


title: HudsonAlpha_ChipSeq_HCT-116_JunD_v042211.1

value: Chromatin IP Sequencing

value: rabbit polyclonal IgG. Antibody Target:JunD

value: The protein encoded by this intronless gene is a member of the JUN family, and a functional component of the AP1 transcription factor complex. It has been proposed to protect cells from p53-dependent senescence and apoptosis. Alternate translation initiation site usage results in the production of different isoforms. (provided by RefSeq)

value: M

value: JunD

tag: replicate


title: HudsonAlpha_ChipSeq_HCT-116_Egr-1_v042211.1

value: Rabbit polyclonal, EGR-1 (588). Antibody Target:EGR1

value: Egr-1, Egr-2, Egr-3 and Egr-4 are nuclear transcription factors belonging to the Egr C2H2-type zinc-finger protein family and containing three C2H2-type zinc fingers. As immediate early proteins, Egr transcription factors are rapidly induced by diverse extracellular stimuli. Egr proteins are subject to tight differential control through diverse mechanisms at several levels of regulation including transcriptional, translational and post-translational (including glyco- sylation, phosphorylation and redox) mechanisms and protein-protein inter- action. Egr-1 binds to the DNA sequence 5'-CGCCCCCGC-3' (EGR-site), there- by activating transcription of target genes whose products are required for mitogenisis and differentiation. Egr-2 binds specific DNA sites located in the promoter region of HoxA4. Egr-2 defects cause congenital hypomyelination neuropathy (also designated Charcot-Marie-tooth disease) and Dejerine- Sottas neuropathology (also designated hereditary motor and sensory neuro- pathy III. Egr-3 is involved in muscle spindle development and is expressed in T cells 20 minutes following activation. EGR-4 binds to the EGR consensus motif GCGTGGGCG, functions as a transcriptional repressor, and displays autoregulatory activities, downregulating its on gene promoter in a dose dependent manner.

value: No special treatment or protocol applies

value: Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)


title: HudsonAlpha_ChipSeq_HL-60_PU.1_v042211.1

value: ChipSeq

value: Rabbit Polyclonal IgG. Antibody Target:PU1

value: F


title: HudsonAlpha_ChipSeq_MCF-7_Egr-1_v042211.1


title: HudsonAlpha_ChipSeq_H1-hESC_TEAD4_(SC-101184)_v042211.1

value: Mouse monoclonal IgG, raised against recombinant protein TEF-3 (TEAD4) of human origin. Antibody Target:TEAD4

value: Member of the transcriptional enhancer factor (TEF) family of transcription factors, which contain the TEA/ATTS DNA-binding domain. It is preferentially expressed in the skeletal muscle, and binds to the M-CAT regulatory element found in promoters of muscle-specific genes to direct their gene expression.


title: HudsonAlpha_ChipSeq_SK-N-SH_FOSL2_v042211.1

value: Rabbit polyclonal. Fra-2(Q-20) is an affinity purified raised against the N-terminus of Fra-2 of human origin. Recommended for detection of Fra-2 of m, r, h and chicken origin by WB, IP, IF, IHC(P) and ELISA. Antibody Target:FOSL2

value: The Fos gene family consists of 4 members:FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation.

value: Fra-2(Q-20) is an affinity purified rabbit polyclonal raised against the N-terminus of Fra-2 of human origin. Recommended for detection of Fra-2 of m, r, h and chicken origin by WB, IP, IF, IHC(P) and ELISA. Antibody Target:FOSL2


title: HudsonAlpha_ChipSeq_ECC-1_NRSF_v042211.1

value: Chromatin IP Sequencing

value: Mouse monoclonal. Antibody Target:NRSF

value: Neuron-restrictive silencer transcription factor

value: Dr. David Anderson at Caltech

value: (Made by Protein Expression Center:pec@caltech.edu)

value: MACS

value: NRSF

value: David Anderson, Caltech


title: HudsonAlpha_ChipSeq_ECC-1_TCF12_v042211.1

value: HEB (A-20) Rabbit polyclonal IgG, 200 ?g/ml. Epitope mapping within the C-terminus of HEB of human origin. Antibody Target:TCF12

value: The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

value: Santa Cruz Biotechnology

value: No special treatment or protocol applies

value: Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)


title: HudsonAlpha_ChipSeq_MCF-7_SRF_v042211.1

value: ChipSeq

value: Rabbit polyclonal. Antibody Target:SRF

value: Serum response transcription factor

value: F

value: SRF


title: HudsonAlpha_ChipSeq_MCF-7_NR2F2_(SC-271940)_v042211.1

value: Chromatin IP Sequencing

value: Mouse monoclonal IgG, specific for an epitope mapping between amino acids 13-44 at the N-terminus of NR2F2 of human origin. Antibody Target:NR2F2

value: This gene encodes a member of the steroid thyroid hormone superfamily of nuclear receptors. The encoded protein is a ligand inducible transcription factor that is involved in the regulation of many different genes. Alternate splicing results in multiple transcript variants. (provided by RefSeq)


title: HudsonAlpha_ChipSeq_MCF-7_PML_(SC-71910)_v042211.1

value: Mouse monoclonal IgG, N-terminal epitope corresponding to residues 37-51 of PML of human origin. Antibody Target:PML

value: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified. (provided by RefSeq)


title: HudsonAlpha_ChipSeq_HCT-116_USF-1_v042211.1

value: Rabbit polyclonal, USF-1 (C-20). Antibody Target:USF1

value: The ubiquitously expressed cellular upstream stimulatory factor (USF) consists of USF-1 and USF-2 polypeptides which independently exhibit site-specific DNA binding and are members of the c-Myc-related family of regulatory factors containing helix-loop-helix domains. USF also contains a leucine repeat that is required for efficient DNA binding. USF was originally identified as an up- stream stimulatory factor that binds the core sequence CACGTG in the adeno- virus late promoter. These findings, together with the demonstration of coop- erative interaction between USF and the initiator-binding protein, TFII-I, raises the possibility of a more general involvement of USF in transcriptional regula- tion. While expression of both USF-1 and USF-2 species is ubiquitous, different ratios of USF homo- and heterodimers are found in different cell types.

value: MACS

value: M


title: HudsonAlpha_ChipSeq_A549_GABP_EtOH_0.02pct_v042211.1

value: Mouse monoclonal, GABPa(G-1), IgG1. Antibody Target:GABP

value: The transcription factor GA-binding protein (GABP) is composed of two subunits, alpha and beta. Alpha binds to a specific DNA sequence.

value: Santa Cruz Biotechnology

value: GABP

value: 1 h with 0.02% Ethanol (Myers)


title: HudsonAlpha_ChipSeq_A549_ZBTB33_EtOH_0.02pct_v042211.1

value: ChipSeq

value: Kaiso (6F8) Mouse monoclonal IgG1. Raised against amino acids 1-504 representing full length Kaiso of human origin. Recommended for detection of Kaiso of m, r, h, canine and chicken origin by WB, IP, IF and ELISA. Antibody Target:ZBTB33

value: KAISO is a transcriptional regulator that binds, via its zinc fingers, to DNA sequences containing methylated CGCG or to the consensus KAISO-binding site (KBS) TCCTGCNA

value: Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)


title: HudsonAlpha_ChipSeq_SK-N-SH_MEF2A_v042211.1

value: Chromatin IP Sequencing

value: Rabbit polyclonal IgG, epitope mapping at the C-terminus of MEF-2 of human origin. Antibody Target:MEF2A

value: The protein encoded by this gene is a DNA-binding transcription factor that activates many muscme-specific, growth factor-induced, and stress-induced genes.

value: F

value: No special treatment or protocol applies


title: HudsonAlpha_ChipSeq_SK-N-SH_Max_v042211.1

value: Rabbit polyclonal IgG, epitope mapping at the C-terminus of Max of human origin. Antibody Target:Max

value: The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Multiple alternatively spliced transcript variants have been described for this gene but the full-length nature for some of them is unknown (RefSeq).

value: Max


title: HudsonAlpha_ChipSeq_HUVEC_Pol2_PCR1x

source_name: HUVEC

value: mouse monoclonal, clone 8WG16. Antibody Target:POL2

value: RNA Polymerase II

value: Covance

value: MACS

value: U

value: one 15-cycle round of PCR (Myers)


title: HudsonAlpha_ChipSeq_A549_p300_EtOH_0.02pct_v042211.1

value: ChipSeq

value: Rabbit Polyclonal. Antibody Target:P300

value: Santa Cruz Biotechnology

value: M

value: 1 h with 0.02% Ethanol (Myers)


title: HudsonAlpha_ChipSeq_A549_ETS1_EtOH_0.02pct_v042211.1

value: Chromatin IP Sequencing

value: Rabbit polyclonal IgG. Antibody Target:ETS1

value: ETS transcriptions factors, such as ETS1, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis

value: Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)


title: HudsonAlpha_ChipSeq_H1-hESC_E2F6_v042211.1

value: Rabbit polyclonal IgG, epitope corresponding to a.a. 232-281 mapping at the C-terminus of E2F-6 of human origin. Antibody Target:E2F6

value: This gene encodes a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses. Many E2F proteins contain several evolutionarily conserved domains:a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The encoded protein of this gene is atypical because it lacks the transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. The protein is part of a multimeric protein complex that contains a histone methyltransferase and the transcription factors Mga and Max. Multiple transcript variants have been reported for this gene, but it has not been clearly demonstrated that they encode valid isoforms (RefSeq).

value: No special treatment or protocol applies


title: HudsonAlpha_ChipSeq_A549_FOXA1_(SC-101058)_DEX_100nM_v041610.2

value: Mouse monoclonal IgG, raised against recombinant HNF-3a of human origin. Antibody Target:FOXA1

value: This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. (provided by RefSeq)

value: Mouse monoclonal IgG, raised against recombinant HNF-3a of human origin. Antibody Target: FOXA1

value: 1 h with 100 nM Dexamethasone (Myers)

value: 2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)


title: HudsonAlpha_ChipSeq_HepG2_ZBTB7A_(SC-34508)_v041610.1

value: ChipSeq

value: Goat polyclonal IgG, epitope mapping at the C-terminus of LRF of human origin. Antibody Target:ZBTB7A

value: Plays a key role in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals (By similarity).

value: MACS

value: Goat polyclonal IgG, epitope mapping at the C-terminus of LRF of human origin. Antibody Target: ZBTB7A

value: 2x10^7 cells, fragmentation by bioruptor, one 15-cycle round of PCR (Myers)


title: HudsonAlpha_ChipSeq_A549_USF-1_EtOH_0.02pct_v042211.1

value: Chromatin IP Sequencing

value: Rabbit polyclonal, USF-1 (C-20). Antibody Target:USF1

value: The ubiquitously expressed cellular upstream stimulatory factor (USF) consists of USF-1 and USF-2 polypeptides which independently exhibit site-specific DNA binding and are members of the c-Myc-related family of regulatory factors containing helix-loop-helix domains. USF also contains a leucine repeat that is required for efficient DNA binding. USF was originally identified as an up- stream stimulatory factor that binds the core sequence CACGTG in the adeno- virus late promoter. These findings, together with the demonstration of coop- erative interaction between USF and the initiator-binding protein, TFII-I, raises the possibility of a more general involvement of USF in transcriptional regula- tion. While expression of both USF-1 and USF-2 species is ubiquitous, different ratios of USF homo- and heterodimers are found in different cell types.

value: Santa Cruz Biotechnology

value: M

value: 1 h with 0.02% Ethanol (Myers)


title: HudsonAlpha_ChipSeq_T-47D_ERalpha_a_BPA_100nM_v041610.2

value: Rabbit affinity purified polyclonal antibody. Epitope is the C-terminus of human estrogen receptor alpha (ESR1). Recommended for detection of estrogen receptor of m, r and h origin by WB, IP, IF, IHC(P) and ELISA. Antibody Target:ESR1

value: This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5-prime UTRs and use different promoters. (provided by RefSeq)

value: 1 h with 100 nM Bisphenol A (Myers)

value: 1 h with 100 nM Bisphenol A  (Myers)


title: HudsonAlpha_ChipSeq_K562_CTCF_(SC-5916)_PCR1x

value: Goat polyclonal IgG, epitope mapping at the N-terminus of CTCF of human origin. Antibody Target:CTCF

value: This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. (provided by RefSeq)

value: F

value: No special treatment or protocol applies

value: one 15-cycle round of PCR (Myers)


title: HudsonAlpha_ChipSeq_HepG2_Pol2-4H8_v041610.2

value: ChipSeq

value: Mouse monoclonal to RNA polymerase II CTD repeat YSPTSPS antibody (4H8) - ChIP Grade. Antibody Target:POL2

value: This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes

value: abcam

value: MACS

value: 2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)


title: HudsonAlpha_ChipSeq_HL-60_GABP_v042211.1

value: Chromatin IP Sequencing

value: Mouse monoclonal, GABPa(G-1), IgG1. Antibody Target:GABP

value: The transcription factor GA-binding protein (GABP) is composed of two subunits, alpha and beta. Alpha binds to a specific DNA sequence.

value: Santa Cruz Biotechnology

value: GABP

value: Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)


title: HudsonAlpha_ChipSeq_PFSK-1_Pol2-4H8_v041610.1

value: M

value: 2x10^7 cells, fragmentation by bioruptor, one 15-cycle round of PCR (Myers)


title: HudsonAlpha_ChipSeq_MCF-7_HDAC2_(SC-6296)_v042211.1

value: Goat polyclonal IgG, epitope mapping at the C-terminus of HDAC2 of human origin. Antibody Target:HDAC2

value: This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. (provided by RefSeq)

value: F


title: HudsonAlpha_ChipSeq_SK-N-SH_Pol2-4H8_v041610.1

value: ChipSeq

value: No special treatment or protocol applies


title: HudsonAlpha_ChipSeq_H1-hESC_RevXlinkChromatin_v042211.1

tag: cell

tag: cell organism

value: human

tag: cell description

value: embryonic stem cells

tag: cell karyotype

value: normal

tag: cell lineage

value: inner cell mass

value: RevXlinkChromatin


title: HudsonAlpha_ChipSeq_HepG2_RevXlinkChromatin_v042211.1

tag: datatype

tag: datatype description

value: Chromatin IP Sequencing

value: hepatocellular carcinoma

value: cancer

value: endoderm

tag: treatment


title: HudsonAlpha_ChipSeq_A549_TAF1_EtOH_0.02pct_v042211.1

tag: antibody antibodydescription

value: Mouse monoclonal (p250). Antibody Target:TAF1

tag: antibody targetdescription

value: TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa. Also designated TAF250.

tag: antibody vendorname

value: Santa Cruz Biotechnology

tag: antibody vendorid

tag: controlid

value: MACS

value: 1 h with 0.02% Ethanol (Myers)

value: Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)


title: HudsonAlpha_ChipSeq_SK-N-SH_Sin3Ak-20_v041610.1

value: ChipSeq

value: Rabbit polyclonal, Sin3A (k-20). Antibody Target:SIN3AK20

value: Co-repressor interacting with HDAC1, N-coR, SMRT, and MeCP2

tag: labexpid

tag: softwareversion

tag: cell sex

value: F

tag: antibody

tag: treatment description

value: 2x10^7 cells, fragmentation by bioruptor, one 15-cycle round of PCR (Myers)


title: HudsonAlpha_ChipSeq_MCF-7_TAF1_v042211.1

value: No special treatment or protocol applies

tag: protocol

tag: protocol description

tag: replicate


title: HudsonAlpha_ChipSeq_HepG2_NR2F2_(SC-271940)_v042211.1

value: Mouse monoclonal IgG, specific for an epitope mapping between amino acids 13-44 at the N-terminus of NR2F2 of human origin. Antibody Target:NR2F2

value: This gene encodes a member of the steroid thyroid hormone superfamily of nuclear receptors. The encoded protein is a ligand inducible transcription factor that is involved in the regulation of many different genes. Alternate splicing results in multiple transcript variants. (provided by RefSeq)

value: M


title: HudsonAlpha_ChipSeq_HUVEC_Pol2-4H8_v041610.1

value: Chromatin IP Sequencing

value: Mouse monoclonal to RNA polymerase II CTD repeat YSPTSPS antibody (4H8) - ChIP Grade. Antibody Target:POL2

value: This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes

value: abcam

value: U


title: HudsonAlpha_ChipSeq_HepG2_ZEB1_(SC-25388)_v042211.1

value: Rabbit polyclonal IgG, epitope corresponding to amino acids 39-140 mapping near the N-terminus of ZEB1 of human origin. Antibody Target:ZEB1

value: This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have ben associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described. (provided by RefSeq).

value: Santa Cruz Biotechnology

value: MACS


title: HudsonAlpha_ChipSeq_ECC-1_Max_v042211.1

value: ChipSeq

value: Rabbit polyclonal IgG, epitope mapping at the C-terminus of Max of human origin. Antibody Target:Max

value: The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Multiple alternatively spliced transcript variants have been described for this gene but the full-length nature for some of them is unknown (RefSeq).

value: Max

value: Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)


title: HudsonAlpha_ChipSeq_H1-neurons_TAF1_v042211.1

value: Mouse monoclonal (p250). Antibody Target:TAF1

value: TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa. Also designated TAF250.

value: No special treatment or protocol applies


title: HudsonAlpha_ChipSeq_H1-neurons_NRSF_v042211.1

value: Chromatin IP Sequencing

value: Mouse monoclonal. Antibody Target:NRSF

value: Neuron-restrictive silencer transcription factor

value: Dr. David Anderson at Caltech

value: (Made by Protein Expression Center:pec@caltech.edu)

value: M

value: NRSF

value: David Anderson, Caltech


title: HudsonAlpha_ChipSeq_HepG2_CREB1_(SC-240)_v042211.1

value: Mouse monoclonal IgG1; raised against amino acids 254-327 mapping within the DNA binding and dimerization domain of human CREB-1. Antibody Target:CREB1

value: This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. (provided by RefSeq)

value: Santa Cruz Biotechnology

value: MACS


title: HudsonAlpha_ChipSeq_SK-N-SH_USF-1_v042211.1

value: ChipSeq

value: Rabbit polyclonal, USF-1 (C-20). Antibody Target:USF1

value: The ubiquitously expressed cellular upstream stimulatory factor (USF) consists of USF-1 and USF-2 polypeptides which independently exhibit site-specific DNA binding and are members of the c-Myc-related family of regulatory factors containing helix-loop-helix domains. USF also contains a leucine repeat that is required for efficient DNA binding. USF was originally identified as an up- stream stimulatory factor that binds the core sequence CACGTG in the adeno- virus late promoter. These findings, together with the demonstration of coop- erative interaction between USF and the initiator-binding protein, TFII-I, raises the possibility of a more general involvement of USF in transcriptional regula- tion. While expression of both USF-1 and USF-2 species is ubiquitous, different ratios of USF homo- and heterodimers are found in different cell types.

value: F


title: HudsonAlpha_ChipSeq_ECC-1_CEBPB_(SC-150)_v042211.1

value: Rabbit polyclonal IgG, epitope mapping at the C-terminus of CEBPB of rat origin. Antibody Target:CEBPB

value: The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene (provided by RefSeq).

value: No special treatment or protocol applies

value: Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)


title: HudsonAlpha_ChipSeq_SK-N-SH_ZBTB33_v042211.1

value: Chromatin IP Sequencing

value: Kaiso (6F8) Mouse monoclonal IgG1. Raised against amino acids 1-504 representing full length Kaiso of human origin. Recommended for detection of Kaiso of m, r, h, canine and chicken origin by WB, IP, IF and ELISA. Antibody Target:ZBTB33

value: KAISO is a transcriptional regulator that binds, via its zinc fingers, to DNA sequences containing methylated CGCG or to the consensus KAISO-binding site (KBS) TCCTGCNA


title: HudsonAlpha_ChipSeq_H1-neurons_Pol2-4H8_v042211.1

value: Mouse monoclonal to RNA polymerase II CTD repeat YSPTSPS antibody (4H8) - ChIP Grade. Antibody Target:POL2

value: This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes

value: abcam

value: M


title: HudsonAlpha_ChipSeq_PANC-1_RevXlinkChromatin_v041610.1

tag: cell

tag: cell organism

value: human

tag: cell description

value: pancreatic carcinoma, (PMID:1140870) PANC-1 was established from a pancreatic carcinoma, which was extracted via pancreatico-duodenectomy specimen from a 56-year-old Caucasian individual. Malignancy of this cell line was verified via in vitro and in vivo assays.

tag: cell karyotype

value: cancer

tag: cell lineage

value: endoderm

value: RevXlinkChromatin

value: 2x10^7 cells, fragmentation by bioruptor, one 15-cycle round of PCR (Myers)


title: HudsonAlpha_ChipSeq_A549_RevXlinkChromatin_EtOH_0.02pct_v042211.1

tag: datatype

value: ChipSeq

tag: datatype description

value: epithelial cell line derived from a lung carcinoma tissue. (PMID:175022), "This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old caucasian male." - ATCC, newly promoted to tier 2:not in 2011 analysis

tag: treatment

value: 1 h with 0.02% Ethanol (Myers)


title: HudsonAlpha_ChipSeq_MCF-7_p300_v042211.1

tag: antibody antibodydescription

value: Rabbit Polyclonal. Antibody Target:P300

tag: antibody targetdescription

tag: antibody vendorname

value: Santa Cruz Biotechnology

tag: antibody vendorid

tag: controlid

value: MACS

value: F

value: No special treatment or protocol applies

value: Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)


title: HudsonAlpha_ChipSeq_A549_RevXlinkChromatin_EtOH_0.02pct_v041610.1

value: Chromatin IP Sequencing

tag: labexpid

tag: cell sex

tag: antibody

tag: treatment description


title: HudsonAlpha_ChipSeq_SK-N-SH_RevXlinkChromatin_v041610.1

tag: cell

value: SK-N-SH

value: neuroblastoma, newly promoted to tier 2:not in 2011 analysis, the SK-N-SH line was established from a neuroblastoma in a 4 year old individual.

value: ectoderm

tag: protocol

tag: protocol description

tag: replicate


title: HudsonAlpha_ChipSeq_SK-N-MC_Pol2-4H8_v041610.1

source_name: SK-N-MC

value: Mouse monoclonal to RNA polymerase II CTD repeat YSPTSPS antibody (4H8) - ChIP Grade. Antibody Target:POL2

value: This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes

value: abcam

tag: softwareversion


title: HudsonAlpha_ChipSeq_MCF-7_Rad21_v042211.1

tag: datatype

value: Rabbit polyclonal. Antibody Target:RAD21

value: Synthetic peptide (Human) conjugated to KLH - which represents a portion of human Rad21 encoded within exon 14


title: HudsonAlpha_ChipSeq_A549_ATF3_EtOH_0.02pct_v042211.1

value: ChipSeq

tag: datatype description

value: Rabbit polyclonal IgG, epitope mapping at the C-terminus of ATF3 of human origin. Antibody Target:ATF3

value: Activating transcription factor 3. A bZIP transcription factor and member of the Ca2+/cAMP response element-binding (CREB) protein family. ATF3 is found to act both as an activator and repressor of transcription.

value: M


title: HudsonAlpha_ChipSeq_PFSK-1_NRSF_v041610.1

value: Mouse monoclonal. Antibody Target:NRSF

value: Neuron-restrictive silencer transcription factor

value: Dr. David Anderson at Caltech

value: (Made by Protein Expression Center:pec@caltech.edu)

value: MACS

value: NRSF

value: David Anderson, Caltech

tag: treatment

value: 2x10^7 cells, fragmentation by bioruptor, one 15-cycle round of PCR (Myers)


title: HudsonAlpha_ChipSeq_HepG2_SP2_(SC-643)_v042211.1

value: Chromatin IP Sequencing

tag: antibody antibodydescription

value: Rabbit polyclonal IgG, epitope mapping near the N-terminus of SP2 of human origin. Antibody Target:SP2

value: This gene encodes a member of the Sp subfamily of Sp/XKLF transcription factors. Sp family proteins are sequence-specific DNA-binding proteins characterized by an amino-terminal trans-activation domain and three carboxy-terminal zinc finger motifs. This protein contains the least conserved DNA-binding domain within the Sp subfamily of proteins, and its DNA sequence specificity differs from the other Sp proteins. It localizes primarily within subnuclear foci associated with the nuclear matrix, and can activate or in some cases repress expression from different promoters. (provided by RefSeq)

value: Santa Cruz Biotechnology

value: No special treatment or protocol applies

value: Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)


title: HudsonAlpha_ChipSeq_A549_ELF1_(SC-631)_EtOH_0.02pct_v042211.1

value: ChipSeq

value: Rabbit polyclonal IgG, epitope mapping at the C-terminus of Elf-1 of human origin. Antibody Target:ELF1

tag: antibody targetdescription

value: This gene encodes an E26 transformation-specific related transcription factor. The encoded protein is primarily expressed in lymphoid cells and acts as both an enhancer and a repressor to regulate transcription of various genes. Alternative splicing results in multiple transcript variants. (provided by RefSeq)

value: Rabbit polyclonal IgG, epitope mapping at the C-terminus of Elf-1 of human origin. Antibody Target: ELF1

value: 1 h with 0.02% Ethanol (Myers)


title: HudsonAlpha_ChipSeq_A549_YY1_(SC-281)_EtOH_0.02pct_v042211.1

value: Rabbit polyclonal IgG, epitope mapping at the C-terminus of YY1 of human origin. Antibody Target:YY1

value: YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1

tag: antibody vendorname

value: M


title: HudsonAlpha_ChipSeq_PANC-1_Sin3Ak-20_v041610.1

value: Rabbit polyclonal, Sin3A (k-20). Antibody Target:SIN3AK20

value: Co-repressor interacting with HDAC1, N-coR, SMRT, and MeCP2

tag: antibody vendorid

value: MACS


title: HudsonAlpha_ChipSeq_MCF-7_GATA3_(SC-268)_v042211.1

value: Chromatin IP Sequencing

value: Mouse monoclonal IgG raised against human recombinant GATA-3. Antibody Target:GATA3

value: This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is a n important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (provided by RefSeq).

tag: controlid

value: F

value: Mouse monoclonal IgG raised against human recombinant GATA-3. Antibody Target: GATA3


title: HudsonAlpha_ChipSeq_K562_NR2F2_(SC-271940)_v042211.1

value: Mouse monoclonal IgG, specific for an epitope mapping between amino acids 13-44 at the N-terminus of NR2F2 of human origin. Antibody Target:NR2F2

value: This gene encodes a member of the steroid thyroid hormone superfamily of nuclear receptors. The encoded protein is a ligand inducible transcription factor that is involved in the regulation of many different genes. Alternate splicing results in multiple transcript variants. (provided by RefSeq)

value: Santa Cruz Biotechnology

tag: labexpid

value: No special treatment or protocol applies

value: Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)


title: HudsonAlpha_ChipSeq_A549_SP1_EtOH_0.02pct_v042211.1

value: ChipSeq

value: Sp1(E-3) is a mouse monoclonal antibody raised against amino acids 121-345 mapping near the N-terminus of Sp1 ofhuman origin. Recommended for detection of Sp1 of m, r and h origin by WB, IP, IF and ELISA. Antibody Target:SP1

value: SP1 is a transcription factor that binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. It can interact with G/C-rich motifs from the serotonin receptor promoter.

tag: cell sex

value: 1 h with 0.02% Ethanol (Myers)


title: HudsonAlpha_ChipSeq_ECC-1_Rad21_v042211.1

value: Rabbit polyclonal. Antibody Target:RAD21

value: Synthetic peptide (Human) conjugated to KLH - which represents a portion of human Rad21 encoded within exon 14

value: abcam

tag: antibody


title: HudsonAlpha_ChipSeq_PFSK-1_RevXlinkChromatin_v041610.1

value: Chromatin IP Sequencing

tag: cell organism

value: human

tag: cell description

value: neuroectodermal cell line derived from a cerebral brain tumor, (PMID:1316433)

tag: cell karyotype

value: cancer

tag: cell lineage

value: ectoderm

value: M

value: RevXlinkChromatin

tag: treatment description

value: 2x10^7 cells, fragmentation by bioruptor, one 15-cycle round of PCR (Myers)


title: HudsonAlpha_ChipSeq_GM12878_ATF2_(SC-81188)_v042211.1

value: Mouse monoclonal IgG, raised against a recombinant protein corresponding to an internal region of ATF2 of human origin. Antibody Target:ATF2

value: This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. The protein forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. The protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Additional transcript variants have been identified but their biological validity has not been determined. (provided by RefSeq)

value: Santa Cruz Biotechnology

tag: softwareversion

value: MACS

value: F

value: No special treatment or protocol applies

tag: protocol

tag: protocol description

tag: replicate


title: HudsonAlpha_ChipSeq_HepG2_CEBPB_(SC-150)_v041610.1

tag: datatype

value: ChipSeq

tag: datatype description

tag: antibody antibodydescription

value: Rabbit polyclonal IgG, epitope mapping at the C-terminus of CEBPB of rat origin. Antibody Target:CEBPB

value: The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene (provided by RefSeq).

tag: treatment


title: HudsonAlpha_ChipSeq_HepG2_CEBPD_(SC-636)_v041610.1

value: Chromatin IP Sequencing

value: Rabbit polyclonal IgG, epitope mapping at the C-terminus of CEBPD of human origin. Antibody Target:CEBPD

tag: antibody targetdescription

value: The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related protein CEBP-alpha. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses, and may be involved in the regulation of genes associated with activation and/or differentiation of macrophages. The cytogenetic location of this locus has been reported as both 8p11 and 8q11 (provided by RefSeq).

tag: antibody vendorname

tag: antibody vendorid

tag: controlid


title: HudsonAlpha_ChipSeq_GM12878_NFATC1_(SC-17834)_v042211.1

value: Mouse monoclonal IgG, raised against amino acids 1-110 of NFATc1 of human origin. Antibody Target:NFATC1

value: The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components:a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Five transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes. (provided by RefSeq)

tag: labexpid

tag: cell sex

tag: antibody

value: Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)


title: HudsonAlpha_ChipSeq_H1-hESC_SP2_(SC-643)_v042211.1

value: Rabbit polyclonal IgG, epitope mapping near the N-terminus of SP2 of human origin. Antibody Target:SP2

value: This gene encodes a member of the Sp subfamily of Sp/XKLF transcription factors. Sp family proteins are sequence-specific DNA-binding proteins characterized by an amino-terminal trans-activation domain and three carboxy-terminal zinc finger motifs. This protein contains the least conserved DNA-binding domain within the Sp subfamily of proteins, and its DNA sequence specificity differs from the other Sp proteins. It localizes primarily within subnuclear foci associated with the nuclear matrix, and can activate or in some cases repress expression from different promoters. (provided by RefSeq)

value: M


title: HudsonAlpha_ChipSeq_PFSK-1_Sin3Ak-20_v041610.1

value: Rabbit polyclonal, Sin3A (k-20). Antibody Target:SIN3AK20

value: Co-repressor interacting with HDAC1, N-coR, SMRT, and MeCP2

value: 2x10^7 cells, fragmentation by bioruptor, one 15-cycle round of PCR (Myers)


title: HudsonAlpha_ChipSeq_SK-N-SH_TAF1_v041610.1

value: Mouse monoclonal (p250). Antibody Target:TAF1

value: TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa. Also designated TAF250.

value: Santa Cruz Biotechnology

value: MACS

value: F


title: HudsonAlpha_ChipSeq_A549_BCL3_EtOH_0.02pct_v042211.1

value: ChipSeq

value: Chromatin IP Sequencing

value: Rabbit polyclonal. Bcl-3(C-14) is an affinity purified antibody raised against a peptide mapping at the C-terminus of Bcl-3 of human origin. Recommended for detection of Bcl-3 of mouse, rat and human origin by WB, IP, IF and ELISA. Antibody Target:BCL3

value: This gene is a proto-oncogene candidate. It is identified by its translocation into the immunoglobulin alpha-locus in some cases of B-cell leukemia. The protein encoded by this gene contains seven ankyrin repeats, which are most closely related to those found in I kappa B proteins. This protein functions as a transcriptional co-activator that activates through its association with NF-kappa B homodimers. The expression of this gene can be induced by NF-kappa B, which forms a part of the autoregulatory loop that controls the nuclear residence of p50 NF-kappa B. (Provided by RefSeq).

value: Bcl-3(C-14) is an affinity purified rabbit polyclonal antibody raised against a peptide mapping at the C-terminus of Bcl-3 of human origin. Recommended for detection of Bcl-3 of m, r and h origin by WB, IP, IF and ELISA. Antibody Target:BCL3

value: 1 h with 0.02% Ethanol (Myers)


title: HudsonAlpha_ChipSeq_HCT-116_TEAD4_(SC-101184)_v042211.1

value: Mouse monoclonal IgG, raised against recombinant protein TEF-3 (TEAD4) of human origin. Antibody Target:TEAD4

value: Member of the transcriptional enhancer factor (TEF) family of transcription factors, which contain the TEA/ATTS DNA-binding domain. It is preferentially expressed in the skeletal muscle, and binds to the M-CAT regulatory element found in promoters of muscle-specific genes to direct their gene expression.

value: No special treatment or protocol applies

value: Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)


title: HudsonAlpha_ChipSeq_ECC-1_CTCF_(SC-5916)_DMSO_0.02pct_v041610.2

value: Goat polyclonal IgG, epitope mapping at the N-terminus of CTCF of human origin. Antibody Target:CTCF

value: This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. (provided by RefSeq)

value: 1 h with 0.02% Dimethyl sufloxide (DMSO) (Myers)

value: 2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)


title: HudsonAlpha_ChipSeq_MCF-7_FOSL2_v042211.1

value: Rabbit polyclonal. Fra-2(Q-20) is an affinity purified raised against the N-terminus of Fra-2 of human origin. Recommended for detection of Fra-2 of m, r, h and chicken origin by WB, IP, IF, IHC(P) and ELISA. Antibody Target:FOSL2

value: The Fos gene family consists of 4 members:FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation.

value: Santa Cruz Biotechnology

value: Fra-2(Q-20) is an affinity purified rabbit polyclonal raised against the N-terminus of Fra-2 of human origin. Recommended for detection of Fra-2 of m, r, h and chicken origin by WB, IP, IF, IHC(P) and ELISA. Antibody Target:FOSL2


title: HudsonAlpha_ChipSeq_PFSK-1_TAF1_v041610.1

value: ChipSeq

value: Chromatin IP Sequencing

value: Mouse monoclonal (p250). Antibody Target:TAF1

value: TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa. Also designated TAF250.

value: MACS

value: M

value: 2x10^7 cells, fragmentation by bioruptor, one 15-cycle round of PCR (Myers)


title: HudsonAlpha_ChipSeq_SK-N-SH_RXRA_v042211.1

value: RXRa(D-20) is an affinity purified rabbit polyclonal antibody raised against a peptide mapping at the N-terminus of RXRalpha of human origin. Recommended for detection of RXRalpha of m, r and h origin by WB, IP, IF, IHC(P) and ELISA. Antibody Target:RXRA

value: Retinoid X receptors (RXRs) and retinoic acid receptors (RARs), are nuclear receptors that mediate the biological effects of retinoids by their involvement in retinoic acid-mediated gene activation. These receptors exert their action by binding, as homodimers or heterodimers, to specific sequences in the promoters of target genes and regulating their transcription. The protein encoded by this gene is a member of the steroid and thyroid hormone receptor superfamily of transcriptional regulators.

value: F

value: RXRA

value: No special treatment or protocol applies


title: HudsonAlpha_ChipSeq_PANC-1_Pol2-4H8_v041610.1

value: Mouse monoclonal to RNA polymerase II CTD repeat YSPTSPS antibody (4H8) - ChIP Grade. Antibody Target:POL2

value: This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes

value: abcam


title: HudsonAlpha_ChipSeq_GM12878_PML_(SC-71910)_v042211.1

value: Mouse monoclonal IgG, N-terminal epitope corresponding to residues 37-51 of PML of human origin. Antibody Target:PML

value: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified. (provided by RefSeq)

value: Santa Cruz Biotechnology

value: Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)


title: HudsonAlpha_ChipSeq_A549_E2F6_v042211.1

value: ChipSeq

value: Chromatin IP Sequencing

value: Rabbit polyclonal IgG, epitope corresponding to a.a. 232-281 mapping at the C-terminus of E2F-6 of human origin. Antibody Target:E2F6

value: This gene encodes a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses. Many E2F proteins contain several evolutionarily conserved domains:a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The encoded protein of this gene is atypical because it lacks the transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. The protein is part of a multimeric protein complex that contains a histone methyltransferase and the transcription factors Mga and Max. Multiple transcript variants have been reported for this gene, but it has not been clearly demonstrated that they encode valid isoforms (RefSeq).


title: HudsonAlpha_ChipSeq_MCF-7_FOXM1_(SC-502)_v042211.1

value: Rabbit polyclonal IgG, epitope mapping at the C-terminus of FOXM1 of human origin. Antibody Target:FOXM1

value: The protein encoded by this gene is a transcriptional activator involved in cell proliferation. The encoded protein is phosphorylated in M phase and regulates the expression of several cell cycle genes, such as cyclin B1 and cyclin D1. Several transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq, Jul 2011)

value: MACS


title: HudsonAlpha_ChipSeq_ECC-1_SRF_v042211.1

value: Rabbit polyclonal. Antibody Target:SRF

value: Serum response transcription factor

value: F

value: SRF


title: HudsonAlpha_ChipSeq_SK-N-SH_p300_v042211.1

value: Rabbit Polyclonal. Antibody Target:P300

value: No special treatment or protocol applies


title: HudsonAlpha_ChipSeq_A549_Rad21_v042211.1

value: Rabbit polyclonal. Antibody Target:RAD21

value: Synthetic peptide (Human) conjugated to KLH - which represents a portion of human Rad21 encoded within exon 14

value: abcam

value: M


title: HudsonAlpha_ChipSeq_HCT-116_ELF1_(SC-631)_v042211.1

value: ChipSeq

value: Rabbit polyclonal IgG, epitope mapping at the C-terminus of Elf-1 of human origin. Antibody Target:ELF1

value: This gene encodes an E26 transformation-specific related transcription factor. The encoded protein is primarily expressed in lymphoid cells and acts as both an enhancer and a repressor to regulate transcription of various genes. Alternative splicing results in multiple transcript variants. (provided by RefSeq)

value: Santa Cruz Biotechnology

value: Rabbit polyclonal IgG, epitope mapping at the C-terminus of Elf-1 of human origin. Antibody Target: ELF1

value: Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)


title: HudsonAlpha_ChipSeq_GM12878_CREB1_(SC-240)_v042211.1

value: Chromatin IP Sequencing

value: Mouse monoclonal IgG1; raised against amino acids 254-327 mapping within the DNA binding and dimerization domain of human CREB-1. Antibody Target:CREB1

value: This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. (provided by RefSeq)

value: MACS


title: HudsonAlpha_ChipSeq_ECC-1_p300_v042211.1

value: F


title: HudsonAlpha_ChipSeq_HCT-116_CBX3_(SC-101004)_v042211.1

value: Mouse monoclonal IgG, raised against recombinant CBX3 of human origin. Antibody Target:CBX3

value: At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.


title: HudsonAlpha_ChipSeq_HCT-116_ATF3_v042211.1

value: Rabbit polyclonal IgG, epitope mapping at the C-terminus of ATF3 of human origin. Antibody Target:ATF3

value: Activating transcription factor 3. A bZIP transcription factor and member of the Ca2+/cAMP response element-binding (CREB) protein family. ATF3 is found to act both as an activator and repressor of transcription.

value: No special treatment or protocol applies


title: HudsonAlpha_ChipSeq_HCT-116_FOSL1_(SC-183)_v042211.1

value: Rabbit polyclonal IgG, epitope mapping at the N-terminus of FOSL1 of human origin. Antibody Target:FOSL1

value: The Fos gene family consists of 4 members:FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. (provided by RefSeq)

value: M

value: Rabbit polyclonal IgG, epitope mapping at the N-terminus of FOSL1 of human origin. Antibody Target: FOSL1


title: HudsonAlpha_ChipSeq_A549_Pbx3_v042211.1

value: ChipSeq

value: Rabbit Polyclonal IgG. Antibody Target:PBX3

value: Santa Cruz Biotechnology


title: HudsonAlpha_ChipSeq_ECC-1_ZBTB7A_(SC-34508)_v042211.1

value: Chromatin IP Sequencing

value: Goat polyclonal IgG, epitope mapping at the C-terminus of LRF of human origin. Antibody Target:ZBTB7A

value: Plays a key role in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals (By similarity).

value: Goat polyclonal IgG, epitope mapping at the C-terminus of LRF of human origin. Antibody Target: ZBTB7A

value: Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)


title: HudsonAlpha_ChipSeq_ECC-1_YY1_(SC-281)_v042211.1

value: Rabbit polyclonal IgG, epitope mapping at the C-terminus of YY1 of human origin. Antibody Target:YY1

value: YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1

value: MACS

value: F


title: HudsonAlpha_ChipSeq_SK-N-SH_FOXM1_(SC-502)_v042211.1

value: Rabbit polyclonal IgG, epitope mapping at the C-terminus of FOXM1 of human origin. Antibody Target:FOXM1

value: The protein encoded by this gene is a transcriptional activator involved in cell proliferation. The encoded protein is phosphorylated in M phase and regulates the expression of several cell cycle genes, such as cyclin B1 and cyclin D1. Several transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq, Jul 2011)

value: No special treatment or protocol applies