title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Atf6 knockdown (T0037), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: CAAGCTAC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Capn2 knockdown (T0036), replicate 1

status: Public on Feb 02 2017

type: SRA

city: Toronto

first: Ulrich

last: Braunschweig

state: Ontario

address: 160 College Street

department: Donnelly Centre

country: Canada

institute: University of Toronto

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: GTTCTGCT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab

library_source: transcriptomic

data_processing:
CASAVA 1.8 was used for base calling.
Fastq files contain demultiplexed reads which were assigned to the appropriate treatment by matching to expected forward and reverse barcodes (sequenced in dedicated reads; not supplied).
Forward and reverse event reads were mapped to custom junction libraries representing all expected splice variants (supplied as processed files), using bowtie with settings --best -v 3 -k 1 --trim3 26 --trim5 20. Trimming was done to remove lower-quality, uninformative ends and because forward reads had a missing base call at position 11 due to a power outage. Junction libraries were first constructed from NCBIm37/mm9 gene annotations, and then refined based on the results of de novo mapping of the reads from one full Illumina lane from each cell line using tophat (default settings with -i 40 and providing Ensembl transcript annotations for NCBIm37), after quality trimming (minimum MAPQ of 33) from the 3'-end with the fastx toolkit. Mapped reads were counted per junction.
Alternative splicing analysis: Percent-spliced-in (PSI) values were calculated for each alternative exon, or part thereof in the case of alternative 5'-/3'-splice sites ('event'), from read tallies as follows: PSI was calculated independently from forward and reverse reads as the percentage of reads supporting inclusion divided by the total number of reads for the event. For alternative 5'/3'-ss events that were part of an alternative exon (Foxm1.A2, Mta1.A1, Uspl1.A3), the PSI was instead calculated with reference to the total reads supporting inclusion of that exon in order to assess independent regulation of alternative splice site usage. Only the read direction in which all reads unambiguously supported either inclusion or exclusion of an event was used, and the average if that applied for both. Exploratory analysis revealed that PSI values showed low-level batch effects per 96-well plate which we moderated by subtracting a weighted plate median in which negative controls (siNT and mock treatment) were given 20x more weight than other samples. To derive variances, and because PSI values are not nearly normally distributed but roughly follow a beta distribution, we elected to fit a beta distribution to each pair of replicate treatments, as well as to all negative controls from both replicates, using maximum-likelihood fitting as implemented in the fitdistr() function from the R package MASS {Venables & Ripley, Modern Applied Statistics with S (2002)}. Iterative optimization of shape parameters was initiated with settings x=PSI, shape1=1, shape2=1, method=”L-BFGS-B”, lower=0.01, and upper=mean number of reads supporting each PSI value. A modified Strictly Standardized Mean Difference (SSMD {Zhang, Genomics (2007)}) was then calculated based on the formula: SSMD = (μt – μc)/SQRT(vart + varc) where μt and μc are the means (corresponding to the PSI), and vart and varc the variances of the beta distributions fitted to the treatment replicates and negative controls, respectively. Events for which not all reads from at least one direction were informative (Mff.A2, Tead1.A2) were excluded from further analysis, as were events in individual treatments with less than 20 reads in one or both replicates (~9% of all event x treatment combinations). Additionally, the following events were removed from further splicing analysis: expression only events or constitutive exons, Fgf4, Gapdh, Srpk2, Srrm4; events with consistently low read counts: Atg13.A1, H2afy.A2, Tcf7l1 (N2A only), Dnmt3b (N2A only); or because measured inclusion was biased by differential length of isoforms:, Fgfr1, Sall4.
Expression analysis: Read counts from all splice variants of each gene were used to estimate relative mRNA expression levels, reads per million reads (RPMs). Differential expression analysis based on raw read counts was performed using the generalized linear models workflow from the R package edgeR. Plate, position (edge/interior) and treatment were used as design factors (where all treatments of each type of control were treated as replicates.) Models fitted using estimateDisp() to estimate the common, trended, and tag-wise dispersion for the CGR8 and N2A data separately. Subsequently, differences attributable to treatment, plate, or position contrasts were extracted with glmLRT() and represented as log2-fold changes with associated FDR. Fold-changes for treatments are relative to the siNT and mock-treatment controls.
Genome_build: NCBIm37/mm9
Supplementary_files_format_and_content: Processed files are either tab-delimited  tables (.tab) or FASTA files (.fa). KnockdownAnnotation.tab contains treatment annotations and well/plate positions. ScreenJuncUp.20-77.tab and ScreenJuncDn.20-77.tab contain the custom junction upstream and downstram junction libraries, respectively, and ScreenEvent_junctions.tab contains the information which junctions were used to calculate PSI for each event. PSI.SSMD.CGR8.tab and PSI.SSMD.N2A.tab contain raw PSI of each replicate and final SSMD. Reads_perEvent_CGR8.tab and Reads_perEvent_N2A.tab contain raw read counts on which expression analyis was based, and Expression.CGR8.treatments.tab and Expression.N2A.treatments.tab contain modeled relative abundance and fold-change with associated FDR for each treatment type.

molecule: total RNA

organism: Mus musculus

source_name: Embryonic stem cells

growth_protocol: CGR8 mouse embryonic stem cells (ESCs) were cultured as described previously on gelatin-coated plates (Gabut et al., Cell (2011), Han  et al., Nature (2013)). Mouse neuroblastoma (N2A) cells were grown in DMEM supplemented with 10% FBS, sodium pyruvate, MEM non-essential amino acids, and penicillin/streptomycin. All cell lines were maintained at 37°C with 5% CO2.

extract_protocol:
Forty-eight hours post-transfection, total RNA were purified from cultured cells using RNeasy Plus 96 Kit (Qiagen), as per the manufacturer’s instructions.
For each sample, a multiplex RT-PCR assay was first applied to simultaneously amplify 50 AS and/or expression events in a single reaction. The multiplex RT-PCR reaction was carried out in 96-well plates using the OneStep RT-PCR kit (Qiagen) as recommended by the manufacturer, with the following changes: reactions were performed in a volume of 20 uL with 2 uL of the purified total RNA as input, and a mixture of 50 pairs of primers was added to each reaction with a final concentration of 0.25 uM for each individual forward and reverse primer. Four identical Veriti 96-well Thermal Cyclers (Applied Biosystems) were used with the following conditions: 50°C for 30 minutes, 95°C for 15 minute, 30 cycles of 94°C for 40 seconds, 58°C for 1 minute (slow ramp rate), 72°C for 3 minutes, and a final extension step at 72°C for 10 minutes. Two sets of barcodes were incorporated into forward and reverse primers, respectively, after the universal adaptors and added to the amplicons in the second PCR reaction to allow demultiplexing all reads that were derived from a particular treatment. Primers sequences were: forward primer, AATGATACGGCGACCACCGAGATCTACACNNNNNNNNACACTCTTTCCCTACACGACGCTCTTCCGATCT (N denotes i5 barcode); reverse primer, CAAGCAGAAGACGGCATACGAGATNNNNNNNNGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT (N denotes i7 primer). The second PCR was performed using Phusion High-Fidelity DNA Polymerase (Thermo Scientific), as per the manufacturer’s instructions. For each 20 uL of reaction, 1 uL of the multiplex RT-PCR reaction products was used as templates. The thermal cycling conditions were as follows: 98°C for 30 seconds, 15 cycles of 98°C for 10 seconds, 65°C for 30 seconds, 72°C for 30 seconds, and a final extension step at 72°C for 5 minutes. The resulting libraries were pooled and sequenced.

treatment_protocol: Twenty-four hours prior to transfection, CGR8 and N2A cells were seeded in 96-well plates, 3000 and 5000 cells per well, respectively. Cells were transfected with the SMARTpool siRNAs at 50 nM final concentration using DharmaFECT1 reagent (Dharmacon), as recommended by the manufacturer.

tag: cell line


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Igf2bp2 knockdown (T0035), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: CCAACCTT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Blm knockdown (T0033), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: TCGAACGT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Srsf11 knockdown (T0031), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: TCCAAGCA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, mock (T0096), replicate 1

description:
Forward (i5) barcode: CTCTCTAT, reverse (i7) barcode: ACCTGTAG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Prpf40b knockdown (T0032), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: TCCTTCGA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Ppp1r8 knockdown (T0034), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: GAAGTCGA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Arid1a knockdown (T0028), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: CTTCTCGA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Banp knockdown (T0030), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: CACATGTG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, mock (T0027), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: TTGGCGAT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Ehmt2 knockdown (T0029), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: CATCAGGT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Acin1 knockdown (T0026), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: TGACACAC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Birc5 knockdown (T0024), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: AGTGCTCA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Snrnp40 knockdown (T0023), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: GCATCGAT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Pds5b knockdown (T0043), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: CGATAACC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Arid3b knockdown (T0019), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: ATTAGGCC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Actl6b knockdown (T0020), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: TCGAGTTG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Nacc1 knockdown (T0021), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: GTTGGTAC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Prdm2 knockdown (T0018), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: ATCCTTGC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Bub3 knockdown (T0025), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: ACACGACT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Cacna1e knockdown (T0016), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: TTGCAACG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Ash1l knockdown (T0014), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: GAGAGACT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Brd4 knockdown (T0015), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: AACCATCG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Gtf2f1 knockdown (T0038), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: CTGTACTC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, siMbnl (T0039), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: TGCAGTAC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Brd3 knockdown (T0013), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: TATTCCGC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Ints7 knockdown (T0017), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: TTGGTAGC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Atf1 knockdown (T0012), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: TCACAGTG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Hjurp knockdown (T0010), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: CTTCGTTG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Akap8l knockdown (T0011), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: TAATCGCG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Atf2 knockdown (T0022), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: TCCTCTAG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Aire knockdown (T0008), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: CCGCTTAT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Tonsl knockdown (T0009), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: CAACACCA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Tfdp2 knockdown (T0004), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: ACAGCAGA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Capn10 knockdown (T0002), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: GACTACTG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Spc25 knockdown (T0003), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: CAAGGATG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Prpf31 knockdown (T0005), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: AGCTTCCT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Atad2 knockdown (T0007), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: CTGTGACA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, Arl6ip4 knockdown (T0006), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: ACAGACAG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: Mll2_shGFP_3

city: Chicago

first: Ali

last: Shilatifard

state: IL

address: 320 E Superior St

department: Department of Biochemistry and Molecular Genetics

country: USA

institute: Northwestern University Feinberg School of Medicine

library_source: genomic

data_processing:
Sequencing data was acquired through the default Illumina pipeline using Casava v1.8.
ChIP-seq reads were aligned to the mouse genome (UCSC mm9) using the Bowtie aligner v0.12.9 allowing uniquely mapping reads only and allowing up to two mismatches (Langmead, 2009). RNA-seq reads were aligned to the mouse genome UCSC mm9 and to gene annotations from Ensembl 67 using TopHat v2.0.13 (Trapnell et al., 2009), using option –g 1 –x 1.
ChIP-seq reads were extended to 150 bases toward the interior of the sequenced fragment and normalized to total reads aligned (reads per million; RPM). RNA-seq reads were normalized to total reads aligned (reads per million; RPM) to generate bigWig files.
Peak detection was done using MACS v1.4.2 (Zhang et al., 2008). Associated control samples were used to determine statistical enrichment at p<1e-5. For down stream analysis, peaks were further filtered with p < 1e-8 and FDR < 0.05.
R package edgeR 3.0.8 was used to perform differential expression analysis at p-value < 1e-3 (Robinson et al., 2010).
Reads were demultiplexed by allowing no mismatch in library index and 1 mismatch in viewpoint sequences. The library index were trimmed before mapping. 4cseqpipe were used for alignment (van de Werken HG et al., 2012). The 4cseqpipe (van de Werken HG et al., 2012) generated figures directly from the alignment files.
Genome_build: mm9
Supplementary_files_format_and_content: BigWig

molecule: genomic DNA

source_name: mouse embryonic stem cells

growth_protocol: Mouse V6.5 ES cells were grown in DMEM supplemented with 15% FBS (HyClone), 2 mM L-glutamine, 0.1 mM nonessential amino acids (Stemcell Technologies) and 1,000 U/ml recombinant LIF (Millipore). Hanging drop method was used to form embryoid bodies (EBs) by culturing 1000 mouse ES cells in 25 µl differentiation medium (without LIF) on the lid of 150 mm culture dish for indicated days.

extract_protocol:
ChIP assays were performed as previously described (Lee et al., 2006) . Briefly, 2-5x10^7 cells were crosslinked with 1% paraformaldehyde (Electron microscropy science #15710) at room temperature for 10 min and sonicated to generate chromatin fragments of 200-600bp. The sheared chromatin was immunoprecipated with a specific antibody. Total RNA was extracted with Trizol (Invitrogen #15596), treated with DNAase I (NEB #M0303) for 20 min at room temperature and purified with RNeasy Mini kit (Qiagen #74106).Circular Chromosome Conformation Capture (4C) technique was performed as described (van de Werken et al., 2012, Methods Enzymol). Cross-linked chromatin was digested with HindIII, ligated under diluted conditions.  Cross links were reversed and DNA was further digested by NlaIII and again ligated under diluted conditions.For each viewpoint approximately 3.2 μg of the 4C-seq library was amplified using 16 individual PCR reactions with inverse primers containing Illumina adaptor sequence. Multiplexed samples were sequenced on the Illumina Nextseq500 system using 75 bp single-end reads according to the manufacturer’s specifications.  4C-seq reads were sorted, aligned, and translated to restriction fragments using the 4C-seq pipeline (van de Werken et al., 2012, Methods Enzymol).
ChIP-seq and RNA-seq libraries were constructed by following instructions in KAPA HTP Library Preparation Kits (Kapa Biosystems, #KK8234) and TruSeq Stranded Total RNA Library Prep kits with Ribo-Zero Human/Mouse/Rat (Illumina, #RS-122-2201 and #RS-122-2202), respectively. Indexed primers for ChIP-seq libraries are from Bioo Scientific (#514104 ). For 4C-seq, Cross-linked chromatin was digested with HindIII, ligated under diluted conditions.  Cross links were reversed and DNA was further digested by NlaIII and again ligated under diluted conditions. For each viewpoint, approximately 3.2 μg of the 4C-seq library was amplified using 16 individual PCR reactions with inverse primers containing Illumina adaptor sequence. Multiplexed samples were sequenced on the Illumina Nextseq500 system using 75 bp single-end reads according to the manufacturer’s specifications.

treatment_protocol: Mouse ES cells were infected with lentivial shRNA against either Mll2 or Menin in the presense of 4ug/ml polybrene for 12 hours and then seleted with 1.5 ug/ml puromycinfor 48h before harvest for ChIP-seq or RNA-seq experiments. For rescue experiment, mouse ES cells were electroporated with indicated plasmids and selected with 400 µg/ml G418 for 3 days.

tag: cell type

value: mES

tag: genotype/variation

value: wild type

tag: chip antibody

value: Mll2 (Homemade, #9173)

tag: lentiviral shrna infection

value: shGFP


title: Input_shMll2_1


title: Mll2_shMenin_3

value: shMenin


title: Prdm14_4C

description: library strategy: 4C-seq

data_processing:
Sequencing data was acquired through the default Illumina pipeline using Casava v1.8.
ChIP-seq reads were aligned to the mouse genome (UCSC mm9) using the Bowtie aligner v0.12.9 allowing uniquely mapping reads only and allowing up to two mismatches (Langmead, 2009). RNA-seq reads were aligned to the mouse genome UCSC mm9 and to gene annotations from Ensembl 67 using TopHat v2.0.13 (Trapnell et al., 2009), using option –g 1 –x 1.
ChIP-seq reads were extended to 150 bases toward the interior of the sequenced fragment and normalized to total reads aligned (reads per million; RPM). RNA-seq reads were normalized to total reads aligned (reads per million; RPM) to generate bigWig files.
Peak detection was done using MACS v1.4.2 (Zhang et al., 2008). Associated control samples were used to determine statistical enrichment at p<1e-5. For down stream analysis, peaks were further filtered with p < 1e-8 and FDR < 0.05.
R package edgeR 3.0.8 was used to perform differential expression analysis at p-value < 1e-3 (Robinson et al., 2010).
Reads were demultiplexed by allowing no mismatch in library index and 1 mismatch in viewpoint sequences. The library index were trimmed before mapping. 4cseqpipe were used for alignment (van de Werken HG et al., 2012). The 4cseqpipe (van de Werken HG et al., 2012) generated figures directly from the alignment files.
Genome_build: mm9

tag: viewpoint


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, siNT (T0001), replicate 1

description:
Forward (i5) barcode: TAGATCGC, reverse (i7) barcode: GTTGACCA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: Input_shMenin_3


title: Mll2_Mll2WT_vector

tag: rescue experiment

value: Halo


title: Mll2_Mll2KO_MLL2_deltaCXXC


title: Mll2_Mll2KO_vector


title: Mll2_Mll2KO_MLL2WT


title: Mll2_Mll2KO_MLL2_Y2604A


title: Mll2_ab_CT2

value: Mll2 (Homemade, #9175)


title: Menin


title: H3K4me3_shMenin_3

value: H3K4me3 (Homemade, #28)


title: H3K4me3_shMll2_1


title: H3K4me3_shGFP_1

data_processing:
Sequencing data was acquired through the default Illumina pipeline using Casava v1.8.
ChIP-seq reads were aligned to the mouse genome (UCSC mm9) using the Bowtie aligner v0.12.9 allowing uniquely mapping reads only and allowing up to two mismatches (Langmead, 2009). RNA-seq reads were aligned to the mouse genome UCSC mm9 and to gene annotations from Ensembl 67 using TopHat v2.0.13 (Trapnell et al., 2009), using option –g 1 –x 1.
ChIP-seq reads were extended to 150 bases toward the interior of the sequenced fragment and normalized to total reads aligned (reads per million; RPM). RNA-seq reads were normalized to total reads aligned (reads per million; RPM) to generate bigWig files.
Peak detection was done using MACS v1.4.2 (Zhang et al., 2008). Associated control samples were used to determine statistical enrichment at p<1e-5. For down stream analysis, peaks were further filtered with p < 1e-8 and FDR < 0.05.
R package edgeR 3.0.8 was used to perform differential expression analysis at p-value < 1e-3 (Robinson et al., 2010).
Reads were demultiplexed by allowing no mismatch in library index and 1 mismatch in viewpoint sequences. The library index were trimmed before mapping. 4cseqpipe were used for alignment (van de Werken HG et al., 2012). The 4cseqpipe (van de Werken HG et al., 2012) generated figures directly from the alignment files.
Genome_build: mm9
Supplementary_files_format_and_content: BigWig, txt


title: Input


title: H3K4me3_shGFP_3


title: H3K4me3_Mll2WT_vector


title: Input_shGFP_3


title: iCLIP-seq size-matched input for Nacc1

description: Extracted RNA was reverse transcribed using primer Rt13clip: /5Phos/NNTCCGNNNAGATCGGAAGAGCGTCGTGgatcCTGAACCGC. Reads start with 5'-NNNCGGANN, where N are random bases.

data_processing:
CASAVA 1.8 was used for base calling.
Reads from two sequencing runs, one with 60 nt and one with 67 nt read length, were trimmed to 49 nt and combined. Reads consisted of 3 random positions, a 4-nt multiplexing barcode, and another 2 random positions, followed by the actual read. Duplicates were discarded.
Reads were de-multiplexed (note that they are already de-multiplexed in this GEO record), and the random positions, barcode, and 3'-bases matching Illumina adaptors were removed.
Remaining longer than 25 nt were mapped to the mouse transcriptome (Ensembl annotation of NCBIm37) using tophat with default settings. To prevent false assignments of reads from repetitive regions, any reads with a mapping quality < 3 were removed from further analysis.
Plots showing average crosslinking signal of events aligned to exon borders were generated as described previously for ChIP-seq data (Braunschweig et al., Genome Res (2014)), except that reads were first reduced to their first position, which is adjacent to the crosslink position, and no normalization against a control was performed. A 21-bp smoothing window average was used for display only.
Genome_build: mm9
Supplementary_files_format_and_content: PDF files contain figures showing the average (across represented exons) CLIP per million in the library, for each aligned base. Plots were generated directly from BAM files.

source_name: Neuroblastoma cells

growth_protocol: Mouse neuroblastoma (N2A) cells were grown in DMEM supplemented with 10% FBS, sodium pyruvate, MEM non-essential amino acids, and penicillin/streptomycin, and maintained at 37°C with 5% CO2.

extract_protocol:
Lysates generated from the crosslinked cells were treated with Turbo DNase (Ambion) and RNase I (1:500; Ambion) for 5 min at 37°C to digest the genomic DNA and trim the RNA to short fragments of an optimal size range. A total of 2% input material was saved to prepare size-matched control libraries. RNA-protein complexes were immunoprecipitated using 100 μl of protein G Dynabeads (Life Technologies) and 10 μg of anti-Nac1 (Abcam). Following stringent high salt washes, the immunoprecipitated RNA was 5' end-labeled using radioactive 32P isotopes. Pre-adenylated adaptors were ligated to the 3' end using the conditions reported by van Nostrand et al. (2016; PMID: 27018577). The immunoprecipitated complexes were separated with SDS-PAGE and transferred to a nitrocellulose membrane (Protran).
RNA was recovered by digesting proteins using proteinase K and then reverse transcribed into cDNA. Reverse transcription primers and barcodes are indicated in the sample metadata. The cDNA was size selected (low – 70 to 85 nt, middle – 85 to 110 nt, high – 110 to 180 nt), circularized to add the adaptor to the 5' end, digested at the internal BamHI site, and then PCR amplified using AccuPrime SuperMix I (Life Technologies). The final PCR libraries were purified on PCR purification columns (Qiagen) to remove residual PCR reagents, and a ratio of 1:5:5 from the low, middle and high fractions were submitted for sequencing.

treatment_protocol: Crosslinking (0.4 J/cm2) was done at 254 nm with a Stratalinker 1800.


title: Input_shGFP_1


title: H3K4me3_Mll2KO_vector


title: H3K4me3_Mll2KO_MLL2_Y2604A


title: iCLIP-seq of Nacc1 in N2A cells, replicate 1

description: Extracted RNA was reverse transcribed using primer Rt9clip: /5Phos/NNGCCANNNAGATCGGAAGAGCGTCGTGgatcCTGAACCGC. Reads start with 5'-NNNTGGCNN, where N are random bases.

tag: antibody

value: Nacc1 antibody (abcam ab29047)


title: iCLIP-seq of Nacc1 in N2A cells, replicate 2

description: Extracted RNA was reverse transcribed using primer Rt10clip: /5Phos/NNGACCNNNAGATCGGAAGAGCGTCGTGgatcCTGAACCGC. Reads start with 5'-NNNGGTCNN, where N are random bases.


title: H3K4me3_Mll2KO_MLL2WT


title: SPAR-Seq of multi-exon regions of 50 genes in CGR8, H3f3b knockdown (T0220), replicate 1

description:
Forward (i5) barcode: GTAAGGAG, reverse (i7) barcode: GCATAACC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.CGR8.tab
Reads_perEvent_CGR8.tab
Expression.CGR8.treatments.tab


title: H3K4me3_Mll2KO_MLL2_deltaCXXC


title: Input_1

data_processing:
Sequencing data was acquired through the default Illumina pipeline using Casava v1.8.
ChIP-seq reads were aligned to the mouse genome (UCSC mm9) using the Bowtie aligner v0.12.9 allowing uniquely mapping reads only and allowing up to two mismatches (Langmead, 2009). RNA-seq reads were aligned to the mouse genome UCSC mm9 and to gene annotations from Ensembl 67 using TopHat v2.0.13 (Trapnell et al., 2009), using option –g 1 –x 1.
ChIP-seq reads were extended to 150 bases toward the interior of the sequenced fragment and normalized to total reads aligned (reads per million; RPM). RNA-seq reads were normalized to total reads aligned (reads per million; RPM) to generate bigWig files.
Peak detection was done using MACS v1.4.2 (Zhang et al., 2008). Associated control samples were used to determine statistical enrichment at p<1e-5. For down stream analysis, peaks were further filtered with p < 1e-8 and FDR < 0.05.
R package edgeR 3.0.8 was used to perform differential expression analysis at p-value < 1e-3 (Robinson et al., 2010).
For 4C seq, reads were demultiplexed by allowing no mismatch in library index and 1 mismatch in viewpoint sequences. The library index were trimmed before mapping. 4cseqpipe were used for alignment (van de Werken HG et al., 2012).
Genome_build: mm9
Supplementary_files_format_and_content: ChIP-seq, RNA-Seq, and 4C samples

extract_protocol:
ChIP assays were performed as previously described (Lee et al., 2006) . Briefly, 2-5x10^7 cells were crosslinked with 1% paraformaldehyde (Electron microscropy science #15710) at room temperature for 10 min and sonicated to generate chromatin fragments of 200-600bp. The sheared chromatin was immunoprecipated with a specific antibody. Total RNA was extracted with Trizol (Invitrogen #15596), treated with DNAase I (NEB #M0303) for 20 min at room temperature and purified with RNeasy Mini kit (Qiagen #74106).Circular Chromosome Conformation Capture (4C) technique was performed as described (van de Werken et al., 2012, Methods Enzymol). Cross-linked chromatin was digested with HindIII, ligated under diluted conditions.  Cross links were reversed and DNA was further digested by NlaIII and again ligated under diluted conditions.For each viewpoint approximately 3.2 μg of the 4C-seq library was amplified using 16 individual PCR reactions with inverse primers containing Illumina adaptor sequence. Multiplexed samples were sequenced on the Illumina Nextseq500 system using 75 bp single-end reads according to the manufacturer’s specifications.  4C-seq reads were sorted, aligned, and translated to restriction fragments using the 4C-seq pipeline according to (van de Werken et al., 2012, Methods Enzymol).
ChIP-seq and RNA-seq libraries were constructed by following instrucitons in KAPA HTP Library Preparation Kits (Kapa Biosystems, #KK8234) and TruSeq Stranded Total RNA Library Prep kits with Ribo-Zero Human/Mouse/Rat (Illumina, #RS-122-2201 and #RS-122-2202), respectively. Indexed primers for ChIP-seq libraries are from Bioo Scientific (#514104 ). For 4C-seq, Cross-linked chromatin was digested with HindIII, ligated under diluted conditions.  Cross links were reversed and DNA was further digested by NlaIII and again ligated under diluted conditions. For each viewpoint, approximately 3.2 μg of the 4C-seq library was amplified using 16 individual PCR reactions with inverse primers containing Illumina adaptor sequence. Multiplexed samples were sequenced on the Illumina Nextseq500 system using 75 bp single-end reads according to the manufacturer’s specifications.

value: V6.5 mES


title: RNA-Seq of N2A cells after non-targeting knockdown (control for Srrm4 and Zfp871 knockdowns), replicate 2

description:
vast-tools.AltSplicing_Srrm4.Zfp871.tab
AltSplicing_Srrm4.Zfp871.tab
Expression_Srrm4.Zfp871.tab

data_processing:
CASAVA 1.8 was used for base calling.
RNA-seq reads were processed for AS and expression analysis using our pipeline vast-tools, version 1.0 (Braunschweig et al., Genome Res. (2014); Irimia et al.,  Cell (2014)), which is available on github (https://github.com/vastgroup/vast-tools).
Alternative splicing analysis: From primary vast-tools output, events with poor coverage or junction balance were filtered out (vast-tools quality column score 3 other than SOK/OK/LOW for cassette exon [CE], microexon [MIC] and alternative 5'/3'-ss events or coverage less than 15 reads for intron retention [IR] events; score 4 other than OK/B1 for CE and MIC events or less than 0.05 for IR events). Significantly differential AS was assessed with the diff module of vast-tools under development and were additionally required to have a PSI difference >10.
Expression analysis: Expression differences were calculated based on vast-tools raw read counts per gene. In cases with a single replicate (knockdown of Nacc1 and Mbnl1/Mbnl2), counts were converted to read-per-million (RPM) and changes calculated as log2((1 + RPM[siSpecific]) / (1 + RPM[siNT])), and genes were required to have a vast-tools cRPKM ≥3 and a raw count of ≥10 in at least one of the compared samples. In cases with multiple replicates (knockdown of Srrm4 and Zfp871), differential expression was assessed with the R package edgeR.
Genome_build: mm9
Supplementary_files_format_and_content: All files are in tab-delimited format. Files vast-tools.AltSplicing_Mbnl.Nacc1.tab and vast-tools.AltSplicing_Srrm4.Zfp871.tab contain primary vast-tools output. Files AltSplicing_Mbnl.Nacc1.tab and AltSplicing_Srrm4.Zfp871.tab contain filtered PSI values and annotations of differential AS. Files Expression_Mbnl.Nacc1.tab and Expression_Srrm4.Zfp871.tab each contain raw counts per gene as well as fold changes and associated p-values, where applicable.

molecule: polyA RNA

extract_protocol:
Total RNA was extracted either with TriZol (ThermoFisher) or the Qiagen RNasy kit.
RNA libraries were prepared for sequencing using standard Illumina protocols

treatment_protocol: Cells were transfected with SMARTpool siRNAs (Dharmacon) using Lipofectamine RNAiMAX (Invitrogen), as recommended by the manufacturer. A non-targeting siRNA pool was used as a control. Cells were harvested 48 hours post transfection.

tag: knockdown

value: siNT


title: RNA-Seq of N2A cells after non-targeting knockdown (control for Srrm4 and Zfp871 knockdowns), replicate 1


title: Mll2_ab_CT1

value: Mll2 (Homemade, #9172)


title: H3K4me1_Mll2WT

value: H3K4me1 (Homemade, #024)


title: RNA-Seq of N2A cells after knockdown of Zfp871, replicate 1


title: H3K4me1_Mll2KO


title: RNA-Seq of N2A cells after non-targeting knockdown (control for Mbnl and Nacc1 knockdowns)

description:
vast-tools.AltSplicing_Mbnl.Nacc1.tab
AltSplicing_Mbnl.Nacc1.tab
Expression_Mbnl.Nacc1.tab


title: RNA-Seq of N2A cells after knockdown of Srrm4, replicate 2


title: RNA-Seq of N2A cells after knockdown of Zfp871, replicate 2


title: ChIP input from N2A cells

data_processing:
CASAVA 1.8 was used for base calling.
Illumina adapter sequences were removed from 3'-ends of 51-nt reads.
Reads were mapped to the mouse genome, release mm9, using bowtie2 with default settings. Duplicate reads were removed with samtools rmdup.
Peaks were jointly called on the IP and input using MACS 1.4.
Genome_build: mm9
Supplementary_files_format_and_content: Nacc1_peaks.bed contains MACS-derived peaks in BED format

extract_protocol:
ChIP (Chromatin Immunoprecipitation) was performed as previously described (Najafabadi et al., Nat. Biotechnol. (2015)). Briefly, ~20 million N2A cells were crosslinked in 1% formaldehyde. Following sonication of DNA fragments, Nacc1 was immunoprecipitated from the lysate with 4ug of Nacc1 antibody (ab29047) followed by crosslink reversal and DNA precipitation.
RNA libraries were prepared for sequencing using standard Illumina protocols


title: RNA-Seq of N2A cells after knockdown of Mbnl1 and Mbnl2


title: iCLIP-seq of Flag-Zfp871 in N2A cells, clone 14, replicate 2

description:
Extracted RNA was reverse transcribed using primer Rt13clip: /5Phos/NNTCCGNNNAGATCGGAAGAGCGTCGTGgatcCTGAACCGC. Reads start with 5'-NNNCGGANN, where N are random bases.
Zfp871_metaExon_UB160331.pdf

data_processing:
CASAVA 1.8 was used for base calling.
51-nt raw reads that consisted of 3 random positions, a 4-nt multiplexing barcode, and another 2 random positions, followed by the actual read were initially trimmed to 49 nt from the 3'-end, and duplicates were discarded.
Reads were de-multiplexed (note that they are already de-multiplexed in this GEO record), and the random positions, barcode, and 3'-bases matching Illumina adaptors were removed.
Remaining longer than 25 nt were mapped to the mouse transcriptome (Ensembl annotation of NCBIm37) using tophat with default settings. To prevent false assignments of reads from repetitive regions, any reads with a mapping quality < 3 were removed from further analysis.
Plots showing average crosslinking signal of events aligned to exon borders were generated as described previously for ChIP-seq data (Braunschweig et al., Genome Res (2014)), except that reads were first reduced to their first position, which is adjacent to the crosslink position, and no normalization against a control was performed. A 21-bp smoothing window average was used for display only.
Genome_build: mm9
Supplementary_files_format_and_content: PDF files contain figures showing the average (across represented exons) CLIP per million in the library, for each aligned base. Plots were generated directly from BAM files.

extract_protocol:
Lysates generated from the crosslinked cells were treated with Turbo DNase (Ambion) and RNase I (1:100, Ambion) for 5 min at 37°C to digest the genomic DNA and trim the RNA to short fragments of an optimal size range. RNA-protein complexes were immunoprecipitated using 100 μl of protein G Dynabeads (Life Technologies) and 10 μg of anti-Flag or 10 μg of anti-Nac1 (Abcam). Following stringent high salt washes, the immunoprecipitated RNA was 5' end-labeled using radioactive 32P isotopes followed by on-bead-ligation of pre-adenylated adaptors to the 3' end. The immunoprecipitated complexes were separated with SDS-PAGE and transferred to a nitrocellulose membrane (Protran).
RNA was recovered by digesting proteins using proteinase K and then reverse transcribed into cDNA. Reverse transcription primers and barcodes are indicated in the sample metadata. The cDNA was size selected (low – 70 to 85 nt, middle – 85 to 110 nt, high – 110 to 180 nt), circularized to add the adaptor to the 5' end, digested at the internal BamHI site, and then PCR amplified using AccuPrime SuperMix I (Life Technologies). The final PCR libraries were purified on PCR purification columns (Qiagen) to remove residual PCR reagents, and a ratio of 1:5:5 from the low, middle and high fractions were submitted for sequencing.

treatment_protocol: Crosslinking (0.15 J/cm2) was done at 254 nm with a Stratalinker 1800.

tag: treatment

value: N2A cells were engineered to express Flag-tagged Zfp871 under the control of doxycyclin using the PiggyBac system, and single-cell subclones were derived. This sample was generated with clone 14. Flag-Zfp871 expression was induced for 24 hours prior to crosslinking.

value: Flag monoclonal antibody M2 (Sigma-Aldrich)


title: iCLIP-seq of Flag-Zfp871 in N2A cells, clone 14, replicate 1

description:
Extracted RNA was reverse transcribed using primer Rt10clip: /5Phos/NNGACCNNNAGATCGGAAGAGCGTCGTGgatcCTGAACCGC. Reads start with 5'-NNNGGTCNN, where N are random bases.
Zfp871_metaExon_UB160331.pdf


title: iCLIP-seq of Flag-Zfp871 in N2A cells, clone 63, replicate 1

description:
Extracted RNA was reverse transcribed using primer Rt1clip: /5Phos/NNAACCNNNAGATCGGAAGAGCGTCGTGgatcCTGAACCGC. Reads start with 5'-NNNGGTTNN, where N are random bases.
Zfp871_metaExon_UB160331.pdf

value: N2A cells were engineered to express Flag-tagged Zfp871 under the control of doxycyclin using the PiggyBac system, and single-cell subclones were derived. This sample was generated with clone 63. Flag-Zfp871 expression was induced for 24 hours prior to crosslinking.


title: iCLIP-seq of Flag-Zfp871 in N2A cells, clone 63 replicate 2

description:
Extracted RNA was reverse transcribed using primer Rt9clip: /5Phos/NNGCCANNNAGATCGGAAGAGCGTCGTGgatcCTGAACCGC. Reads start with 5'-NNNTGGCNN, where N are random bases.
Zfp871_metaExon_UB160331.pdf


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, untreated (T1535), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: ATGGTTCG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, mock (T1536), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: TGGAAGGT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: RNA-Seq of N2A cells after knockdown of Srrm4, replicate 1


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zscan12 knockdown (T1529), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: GGTACGAA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp295 knockdown (T1528), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: ACTGAGTC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, untreated (T1534), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: ATCCATCC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, untreated (T1533), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: TCGACATG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp617 knockdown (T1527), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: TAGCTTCC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, untreated (T1524), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: ACGATGGA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, untreated (T1523), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: TCACTGTC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zc3h8 knockdown (T1525), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: GGCCTATA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: RNA-Seq of N2A cells after knockdown of Nacc1


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Ube2a knockdown (T1532), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: TCCACTAC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zbtb43 knockdown (T1526), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: CAAGGTTC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zscan21 knockdown (T1520), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: CATCCAAG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp628 knockdown (T1517), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: TATAGGCC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp326 knockdown (T1518), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: CTCTGAGA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: ChIP of Nacc1 in N2A cells


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zc3h3 knockdown (T1519), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: GCTATTCC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp282 knockdown (T1516), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: GAGTTGAG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Rbmxl2 knockdown (T1521), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: AGACCTCA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp263 knockdown (T1515), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: GCATCGTA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp236 knockdown (T1513), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: GTACCATG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Ythdf3 knockdown (T1514), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: CGTTATGG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, untreated (T1512), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: CGAAGGAT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, untreated (T1510), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: ACCAGTTG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, untreated (T1511), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: CTTGCATC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp553 knockdown (T1531), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: CAGATGTC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp97 knockdown (T1530), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: ATATCGCG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Rbm44 knockdown (T1509), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: AGACTCTG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, untreated (T1522), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: GGAATAGC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Xab2 knockdown (T1507), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: TGTCGTGA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp358 knockdown (T1508), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: AGACAGAC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Parp12 knockdown (T1506), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: GTGATCTG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Unk knockdown (T1501), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: AATTGCGC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, untreated (T1499), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: CAAGTCCA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp57 knockdown (T1502), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: CCATGCTT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp451 knockdown (T1504), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: CTGTAGAC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp180 knockdown (T1495), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: GTACACCA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, untreated (T1500), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: AGTGCTGT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp36 knockdown (T1490), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: AACCGCTA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Cxcl12 knockdown (T1494), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: GAGAACAC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp36l2 knockdown (T1489), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: GATGCTTG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp68 knockdown (T1492), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: TTGCGGTA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zc3hav1 knockdown (T1491), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: AGCAGAAC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zzz3 knockdown (T1493), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: TGTGAGAG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, untreated (T1486), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: CTTGGATG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, untreated (T1488), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: TCTGAGTG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, siMbnl (T1497), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: ATCCGGAT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, siNT (T1485), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: CTCAGTGA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, untreated (T1498), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: TACGAAGG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp62 knockdown (T1484), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: CAACTGGT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp277 knockdown (T1483), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: TTCCGGAA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp316 knockdown (T1482), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: GAAGTCCT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp955a knockdown (T1481), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: GTTCCTAC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, siMbnl (T1479), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: TGACCTGA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Wbp4 knockdown (T1505), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: GAACAGGT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp260 knockdown (T1480), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: TGTCTGTC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp207 knockdown (T1478), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: GTGACAGA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp13 knockdown (T1477), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: TTGGTTGG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, untreated (T1487), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: GTCTACTC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, untreated (T1476), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: GGATCGTT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfr knockdown (T1471), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: GACATCTG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Nova1 knockdown (T1473), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: AGCACAAG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, untreated (T1475), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: GCGCTAAT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, untreated (T1474), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: ATGCTAGC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zcchc8 knockdown (T1470), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: CATGCTAG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp317 knockdown (T1469), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: GAGTAGTG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp239 knockdown (T1468), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: ACAGGTCT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, mock (T1467), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: TCGACTAG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp330 knockdown (T1458), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: GCTTGGTA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Wtap knockdown (T1466), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: TCACACAG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zrsr2 knockdown (T1460), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: GATGTGGA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Ythdf2 knockdown (T1465), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: TCAGCAGT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Mex3a knockdown (T1461), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: CTACACGA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, untreated (T1464), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: TACCTTCG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp385a knockdown (T1456), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: CCTATTCG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, untreated (T1463), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: CAGTGACT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Ythdf1 knockdown (T1454), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: GGTAGGAT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, untreated (T1452), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: TGCAGATC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, untreated (T1462), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: TCGTGAAC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp467 knockdown (T1459), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: TTCGATCC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Sf1 knockdown (T1457), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: GACACTCA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, U2af2 knockdown (T1472), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: ATCGCGTA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zc3h7a knockdown (T1455), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: CCAACGAT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, untreated (T1450), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: GTGTGTCA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp1 knockdown (T1453), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: TGGAACGA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zbtb22 knockdown (T1448), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: ACCTCTAC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, untreated (T1451), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: GAGTTCAC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Ybx2 knockdown (T1447), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: TATTCGCC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp59 knockdown (T1442), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: TGGTCATG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp35 knockdown (T1446), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: CCAAGCTA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zkscan3 knockdown (T1445), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: ACGAAGCA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp46 knockdown (T1443), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: TTAAGCGC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, siNT (T1441), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: AGCTTGCA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, U2af1l4 knockdown (T1437), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: GCTATAGC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, mock (T1440), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: ATATCGGC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Stag1 knockdown (T1439), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: TATACCGG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Tnrc6a knockdown (T1438), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: TGTGCTGA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Snrpe knockdown (T1435), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: CACTTCTG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Alyref knockdown (T1436), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: CCATGCAA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp9 knockdown (T1503), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: CGTTCCAT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Stag2 knockdown (T1432), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: AACGTTGC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Stag3 knockdown (T1430), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: AACCAAGG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp90 knockdown (T1496), replicate 2

description:
Forward (i5) barcode: TGATACAT, reverse (i7) barcode: TCACGACA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Thoc3 knockdown (T1434), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: AGGTAGGT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Tmpo knockdown (T1428), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: ACTGGACT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Tipin knockdown (T1431), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: CTGTCAGA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zfp319 knockdown (T1444), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: GGAATACG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Thap1 knockdown (T1433), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: ATTAGCCG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Tnks knockdown (T1429), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: ACTGAGAG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Snrpg knockdown (T1426), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: ATCGCCTT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Srsf9 knockdown (T1425), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: CAACCAAC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Zmym1 knockdown (T1449), replicate 2

description:
Forward (i5) barcode: TATGGAGG, reverse (i7) barcode: AGGACTTC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Sox11 knockdown (T1423), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: CGGCTTAA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Sugp2 knockdown (T1424), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: TCAGGTCA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Smc6 knockdown (T1422), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: TCTCCACA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Srrm1 knockdown (T1421), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: TTCGATGG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Smc2 knockdown (T1417), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: TCCTTGGT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Snrpa knockdown (T1419), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: CCGCAATA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Rad54l2 knockdown (T1418), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: GCTAGCTA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Shprh knockdown (T1411), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: GCTTCGTT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, U2af1 knockdown (T1412), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: TCCTGTTG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Sp6 knockdown (T1415), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: CAGTTGAC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Taf9 knockdown (T1413), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: GTACTGGT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Srsf1 knockdown (T1409), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: CGTTGCTT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Kdm6a knockdown (T1410), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: GGAACGAT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Rsph1 knockdown (T1407), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: GTCTTCAC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Supt16h knockdown (T1408), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: AGTCGTGT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Srsf6 knockdown (T1420), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: GTTGCTTC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Vmn1r11 knockdown (T1405), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: CGAAGGTA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Usp39 knockdown (T1404), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: TTGCTTGC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Txnl4a knockdown (T1406), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: CTCTAGAG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Snapc4 knockdown (T1402), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: GACTAGTC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Tra2b knockdown (T1403), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: TCTCTCAG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Sugp1 knockdown (T1427), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: GAACGTAG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Ssb knockdown (T1399), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: TGGAGATG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Sgol1 knockdown (T1398), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: ACCAGAAG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Cenpk knockdown (T1400), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: TGACTGTG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Nhp2l1 knockdown (T1414), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: AAGCGGTA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Scand1 knockdown (T1416), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: CCTAATCC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Snrpd1 knockdown (T1394), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: AGAGACTG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Sept8 knockdown (T1397), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: TTCGCGAA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Sox13 knockdown (T1396), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: AGGAGTAC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, siMbnl (T1401), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: ACGATCGT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Stau1 knockdown (T1392), replicate 2

description:
Forward (i5) barcode: GAGTCAGT, reverse (i7) barcode: TCCAGAAC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, siNT (T1389), replicate 2

description:
Forward (i5) barcode: GAGTCAGT, reverse (i7) barcode: CAGTCTCT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Sin3b knockdown (T1387), replicate 2

description:
Forward (i5) barcode: GAGTCAGT, reverse (i7) barcode: CACAGAGA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Adad1 knockdown (T1395), replicate 2

description:
Forward (i5) barcode: GCTGATCG, reverse (i7) barcode: TTCCTACG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Snrnp70 knockdown (T1388), replicate 2

description:
Forward (i5) barcode: GAGTCAGT, reverse (i7) barcode: GTAGTGGA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, siMbnl (T1383), replicate 2

description:
Forward (i5) barcode: GAGTCAGT, reverse (i7) barcode: ATGGAAGC
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Ttc14 knockdown (T1384), replicate 2

description:
Forward (i5) barcode: GAGTCAGT, reverse (i7) barcode: GTCTTGAG
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Clasrp knockdown (T1386), replicate 2

description:
Forward (i5) barcode: GAGTCAGT, reverse (i7) barcode: CGTACGTA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Sf3a2 knockdown (T1390), replicate 2

description:
Forward (i5) barcode: GAGTCAGT, reverse (i7) barcode: GGAAGGTT
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Spag5 knockdown (T1381), replicate 2

description:
Forward (i5) barcode: GAGTCAGT, reverse (i7) barcode: CGAAGCAA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Sf3b1 knockdown (T1382), replicate 2

description:
Forward (i5) barcode: GAGTCAGT, reverse (i7) barcode: GAGTCAGA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab


title: SPAR-Seq of multi-exon regions of 50 genes in N2A, Thap4 knockdown (T1379), replicate 2

description:
Forward (i5) barcode: GAGTCAGT, reverse (i7) barcode: AAGCGCAA
KnockdownAnnotation.tab
ScreenJuncUp.20-77.fa
ScreenJuncDn.20-77.fa
ScreenEvents_junctions.tab
PSI.SSMD.N2A.tab
Reads_perEvent_N2A.tab
Expression.N2A.treatments.tab