title: old_C57BL6_STHSC_biol_replicate_177

description:
ST_HSC_old_plate3_biol_rep_TCL_G6
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: sug HSD 8h repB

status: Public on Sep 30 2015

type: SRA

city: Helsinki

first: Ville

last: Hietakangas

address: Viikinkaari 9

country: Finland

institute: University of Helsinki

library_source: transcriptomic

data_processing:
Alignment to reference genome was done with BWA v.0.5.9 with default parameters.
Reads with MAPQ >= 10 were selected to further processing with Samtools.
Exon level read counts were processed with HTSeq 0.6.1 using MAPQ >= 10 reads.
Genome_build: FlyBase Drosophila melanogaster r5.54
Supplementary_files_format_and_content: Tab-delimined txt file contains read exon counts for each sample

molecule: total RNA

organism: Drosophila melanogaster

source_name: sugarbabe null mutant (sug17d/sug def) 3rd instar high sugar diet

growth_protocol: Control and sugarbabe knockdown flies were kept in egg laying champers with apple juice plates and yeast paste for 3 hours. 24 hours later the 1st instar larvae were transferred to 10% yeast plates. 24 hours later larvae were transferred to either a 10% yeast + 20% sucrose or a fresh 10% yeast plate. Non-feeding larvae were removed after 1.5 hours. Low sugar samples were collected at the 1.5h time point and high sugar samples were collected 8 hours after the transfer.

extract_protocol:
Larvae were frozen in liquid nitrogen and homogenized with a disposable plastic pestle in lysis buffer and vortexed. Samples were frozen for complete lysis of the tissue. Total RNA was extracted with Nucleospin RNA II kit according to manufacturer's protocol. Elution was done with water and the samples stored at -80°C.
Library contruction was done using standard Illumina stranded mRNA protocol.

tag: genotype

tag: tissue

value: whole larvae

tag: developmental stage

value: 3rd instar


title: control LSD 1.5h repB

source_name: control heterozygous (sug17d/+) 3rd instar control diet


title: young_C57BL6_MPP_2

city: Cambridge

first: Monika

middle: Sylwia

last: Kowalczyk

state: MA

address: 415 Main Street

country: USA

institute: Broad Institute

description:
young_MPP_2
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx

data_processing:
Paired-end 25bp reads were mapped to the UCSC mouse transcriptome by Bowtie, allowing up to two mismatches, and expression levels of all UCSC genes were estimated by RSEM with default parameters. TPM values as defined by RSEM were added a value of 1 (to avoid zeros) and then log2-transformed.
We filtered out cells in which less than 2500 UCSC genes were detected above a log2(TPM+1) threshold of 2. We excluded genes whose log2(TPM+1) was lower than 4 in the aggregared data for each of the 6 populations, and those UCSC genes that were not associated with a HUGO gene Symbol, leaving 7823 genes for the final dataset.
Genome_build: mm9
Supplementary_files_format_and_content: GEO_HSC_single_data.xlsx is an Excel sheet that contain the log-transformed expression levels, log2(TMP+1) of 8422 mouse transcripts in 1429 single cells and in six population averages, including young and old LT-HSCs, ST-HSCs and MPPs. The first row contains cell ID (a unique index for each single cell) or "pop" for the population averages. The second row indicates the type of population (age and cell type) that the cell (or population average) belongs to. The first and second columns include gene symbols and UCSC transcript names, respectively. (The processed data file only represents samples that were of sufficient quality to analyze)

organism: Mus musculus

source_name: hematopoietic stem and progenitor cells

growth_protocol: cells were profiled immediately

extract_protocol:
for single cells - direct lysis; for populations we used PrepEase (Affymetrix) according to manufacturers protocol.
cDNA from each sample was prepared using the SMARTer cDNA synthesis (Clontech). RNA libraries were prepared for sequencing using Nextera XT (Illumina).

tag: strain

tag: age

value: 2-3 months old

tag: cell type

value: multipotent progenitor


title: young_C57BL6_MPP_3

description:
young_MPP_3
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_MPP_1

description:
young_MPP_1
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_171

description:
ST_HSC_old_plate3_biol_rep_TCL_D1
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx

value: 22 months old

value: short term hematopoietic stem cell


title: sug HSD 8h repC


title: sug LSD 1.5h repA

source_name: sugarbabe null mutant (sug17d/sug def) 3rd instar control diet


title: sug LSD 1.5h repB


title: control HSD 8h repA

source_name: control heterozygous (sug17d/+) 3rd instar high sugar diet


title: old_C57BL6_STHSC_biol_replicate_173

description:
ST_HSC_old_plate3_biol_rep_TCL_B1
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_172

description:
ST_HSC_old_plate3_biol_rep_TCL_H1
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: control LSD 1.5h repA


title: young_C57BL6_STHSC_192

description:
young_ST_HSC_192
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_170

description:
ST_HSC_old_plate3_biol_rep_TCL_F1
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_169

description:
ST_HSC_old_plate3_biol_rep_TCL_G1
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_186

description:
ST_HSC_old_plate3_biol_rep_TCL_F4
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_190

description:
young_ST_HSC_190
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: sug HSD 8h repA


title: old_C57BL6_STHSC_biol_replicate_167

description:
ST_HSC_old_plate3_biol_rep_TCL_A12
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_189

description:
young_ST_HSC_189
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_191

description:
young_ST_HSC_191
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_168

description:
ST_HSC_old_plate3_biol_rep_TCL_C12
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_188

description:
young_ST_HSC_188
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_165

description:
ST_HSC_old_plate3_biol_rep_TCL_B12
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_186

description:
young_ST_HSC_186
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: Pear fruit developmental stage 4

city: Hefei

first: cong

middle: bing

last: fang

state: Anhui

address: No. 130 Changjiang West Road

department: School of Horticulture

country: China

institute: Anhui Agricultural University

library_source: non-genomic

data_processing: Raw sequences were transformed into clean tags through removing adaptor sequence,  low-quality sequences (tags with unknown sequences [“N”]), empty reads (sequence  with only adaptor sequences but no tags), too-long or too-short tags, and tags with a copy number of 1 (probably a sequencing error). A preprocessed database of all possible CATG+ 17-nucleotide tag sequences was created using our transcriptome reference database. For annotation, all tags were mapped to the reference sequences and only allowed no more than 1 nucleotide mismatch. Clean tags mapped to reference sequences from multiple genes were filtered,and the remaining clean tags were designed as unambiguous tags. For gene expression analysis, the number of unambiguous clean tags for each gene was calculated and then normalized to the number of transcripts per million tags (TPM). The gene ontology (GO) classification system was used to determine the possible functions of all genes tagged.

organism: Pyrus x bretschneideri

source_name: Shoots, young leaves, extending leaves, mature leaves, flowers and fruit (containing immature seeds)

growth_protocol: Plants were grown under normal orchard conditions.

extract_protocol: Beads with Oligo(dT) are used to isolate poly(A) mRNA after total RNA is collected from pear fruits . Fragmentation buffer is added for interrupting mRNA to short fragments. Taking these short fragments as templates, random hexamer-primer is used to synthesize the first-strand cDNA. The second-strand cDNA is synthesized using buffer, dNTPs, RNaseH and DNA polymerase I, respectively. Short fragments are purified with QiaQuick PCR extraction kit and resolved with EB buffer for end reparation and adding poly(A). After that, the short fragments are connected with sequencing adapters. And, after the agarose gel electrophoresis, the suitable fragment are selected for the PCR amplification as templates. At last, the library could be sequenced using Illumina HiSeq™ 2000.

value: Pyrus bretschneideri Rehd. cv. Dangshansuli

value: pear fruit developmental stage 4


title: old_C57BL6_STHSC_biol_replicate_166

description:
ST_HSC_old_plate3_biol_rep_TCL_E12
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_185

description:
young_ST_HSC_185
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_184

description:
young_ST_HSC_184
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: Pear fruit developmental stage 5

value: Shoots, young leaves, extending leaves, mature leaves, flowers and fruit (containing immature seeds)

value: pear fruit developmental stage 5


title: Pear fruit developmental stage 1

value: pear fruit developmental stage 1


title: old_C57BL6_STHSC_biol_replicate_162

description:
ST_HSC_old_plate3_biol_rep_TCL_F12
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: Pear fruit developmental stage 3

value: pear fruit developmental stage 3


title: young_C57BL6_STHSC_187

description:
young_ST_HSC_187
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_164

description:
ST_HSC_old_plate3_biol_rep_TCL_H12
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: Pear fruit developmental stage 2

value: pear fruit developmental stage 2


title: young_C57BL6_STHSC_183

description:
young_ST_HSC_183
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_161

description:
ST_HSC_old_plate3_biol_rep_TCL_G12
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_182

description:
young_ST_HSC_182
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_159

description:
ST_HSC_old_plate3_biol_rep_TCL_A5
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_181

description:
young_ST_HSC_181
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_180

description:
young_ST_HSC_180
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_158

description:
ST_HSC_old_plate3_biol_rep_TCL_E5
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_179

description:
young_ST_HSC_179
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_157

description:
ST_HSC_old_plate3_biol_rep_TCL_B5
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_155

description:
ST_HSC_old_plate3_biol_rep_TCL_D5
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_156

description:
ST_HSC_old_plate3_biol_rep_TCL_H5
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_177

description:
young_ST_HSC_177
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_178

description:
young_ST_HSC_178
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_163

description:
ST_HSC_old_plate3_biol_rep_TCL_D12
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_176

description:
young_ST_HSC_176
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_154

description:
ST_HSC_old_plate3_biol_rep_TCL_F5
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_175

description:
young_ST_HSC_175
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_149

description:
ST_HSC_old_plate3_biol_rep_TCL_B9
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_172

description:
young_ST_HSC_172
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_148

description:
ST_HSC_old_plate3_biol_rep_TCL_H9
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_152

description:
ST_HSC_old_plate3_biol_rep_TCL_C9
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_171

description:
young_ST_HSC_171
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_151

description:
ST_HSC_old_plate3_biol_rep_TCL_A9
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_173

description:
young_ST_HSC_173
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_174

description:
young_ST_HSC_174
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_150

description:
ST_HSC_old_plate3_biol_rep_TCL_E9
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_160

description:
ST_HSC_old_plate3_biol_rep_TCL_C5
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_146

description:
ST_HSC_old_plate3_biol_rep_TCL_F9
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_168

description:
young_ST_HSC_168
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_145

description:
ST_HSC_old_plate3_biol_rep_TCL_G9
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_147

description:
ST_HSC_old_plate3_biol_rep_TCL_D9
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_167

description:
young_ST_HSC_167
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_166

description:
young_ST_HSC_166
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_144

description:
ST_HSC_old_plate3_biol_rep_TCL_C7
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_164

description:
young_ST_HSC_164
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_142

description:
ST_HSC_old_plate3_biol_rep_TCL_E7
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_143

description:
ST_HSC_old_plate3_biol_rep_TCL_A7
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_163

description:
young_ST_HSC_163
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_153

description:
ST_HSC_old_plate3_biol_rep_TCL_G5
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_141

description:
ST_HSC_old_plate3_biol_rep_TCL_B7
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_165

description:
young_ST_HSC_165
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_162

description:
young_ST_HSC_162
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_170

description:
young_ST_HSC_170
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_169

description:
young_ST_HSC_169
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_140

description:
ST_HSC_old_plate3_biol_rep_TCL_H7
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_161

description:
young_ST_HSC_161
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_137

description:
ST_HSC_old_plate3_biol_rep_TCL_G7
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_160

description:
young_ST_HSC_160
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_159

description:
young_ST_HSC_159
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_135

description:
ST_HSC_old_plate3_biol_rep_TCL_A2
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_138

description:
ST_HSC_old_plate3_biol_rep_TCL_F7
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_156

description:
young_ST_HSC_156
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_134

description:
ST_HSC_old_plate3_biol_rep_TCL_E2
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_136

description:
ST_HSC_old_plate3_biol_rep_TCL_C2
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_158

description:
young_ST_HSC_158
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_133

description:
ST_HSC_old_plate3_biol_rep_TCL_B2
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_132

description:
ST_HSC_old_plate3_biol_rep_TCL_H2
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_154

description:
young_ST_HSC_154
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_153

description:
young_ST_HSC_153
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_131

description:
ST_HSC_old_plate3_biol_rep_TCL_D2
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_152

description:
young_ST_HSC_152
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_151

description:
young_ST_HSC_151
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_130

description:
ST_HSC_old_plate3_biol_rep_TCL_F2
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_129

description:
ST_HSC_old_plate3_biol_rep_TCL_G2
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_150

description:
young_ST_HSC_150
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_139

description:
ST_HSC_old_plate3_biol_rep_TCL_D7
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_127

description:
ST_HSC_old_plate3_biol_rep_TCL_A10
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_148

description:
young_ST_HSC_148
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_128

description:
ST_HSC_old_plate3_biol_rep_TCL_C10
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_149

description:
young_ST_HSC_149
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_125

description:
ST_HSC_old_plate3_biol_rep_TCL_B10
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_146

description:
young_ST_HSC_146
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_145

description:
young_ST_HSC_145
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_123

description:
ST_HSC_old_plate3_biol_rep_TCL_D10
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_124

description:
ST_HSC_old_plate3_biol_rep_TCL_H10
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_155

description:
young_ST_HSC_155
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_147

description:
young_ST_HSC_147
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_144

description:
young_ST_HSC_144
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_142

description:
young_ST_HSC_142
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_121

description:
ST_HSC_old_plate3_biol_rep_TCL_G10
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_157

description:
young_ST_HSC_157
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_126

description:
ST_HSC_old_plate3_biol_rep_TCL_E10
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_122

description:
ST_HSC_old_plate3_biol_rep_TCL_F10
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_120

description:
ST_HSC_old_plate3_biol_rep_TCL_C8
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_119

description:
ST_HSC_old_plate3_biol_rep_TCL_A8
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_141

description:
young_ST_HSC_141
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_118

description:
ST_HSC_old_plate3_biol_rep_TCL_E8
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_140

description:
young_ST_HSC_140
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_143

description:
young_ST_HSC_143
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_138

description:
young_ST_HSC_138
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_139

description:
young_ST_HSC_139
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_136

description:
young_ST_HSC_136
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_114

description:
ST_HSC_old_plate3_biol_rep_TCL_F8
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_115

description:
ST_HSC_old_plate3_biol_rep_TCL_D8
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_135

description:
young_ST_HSC_135
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_113

description:
ST_HSC_old_plate3_biol_rep_TCL_G8
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_134

description:
young_ST_HSC_134
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_112

description:
ST_HSC_old_plate3_biol_rep_TCL_C3
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_111

description:
ST_HSC_old_plate3_biol_rep_TCL_A3
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_133

description:
young_ST_HSC_133
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_132

description:
young_ST_HSC_132
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_131

description:
young_ST_HSC_131
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_107

description:
ST_HSC_old_plate3_biol_rep_TCL_D3
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_108

description:
ST_HSC_old_plate3_biol_rep_TCL_H3
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_109

description:
ST_HSC_old_plate3_biol_rep_TCL_B3
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_106

description:
ST_HSC_old_plate3_biol_rep_TCL_F3
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_126

description:
young_ST_HSC_126
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_129

description:
young_ST_HSC_129
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_128

description:
young_ST_HSC_128
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_130

description:
young_ST_HSC_130
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_127

description:
young_ST_HSC_127
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_105

description:
ST_HSC_old_plate3_biol_rep_TCL_G3
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_116

description:
ST_HSC_old_plate3_biol_rep_TCL_H8
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_103

description:
ST_HSC_old_plate3_biol_rep_TCL_A11
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_117

description:
ST_HSC_old_plate3_biol_rep_TCL_B8
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_125

description:
young_ST_HSC_125
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_101

description:
ST_HSC_old_plate3_biol_rep_TCL_B11
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_124

description:
young_ST_HSC_124
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_137

description:
young_ST_HSC_137
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_102

description:
ST_HSC_old_plate3_biol_rep_TCL_E11
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_122

description:
young_ST_HSC_122
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_123

description:
young_ST_HSC_123
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_121

description:
young_ST_HSC_121
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_100

description:
ST_HSC_old_plate3_biol_rep_TCL_H11
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_99

description:
ST_HSC_old_plate3_biol_rep_TCL_D11
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_110

description:
ST_HSC_old_plate3_biol_rep_TCL_E3
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_119

description:
young_ST_HSC_119
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_97

description:
ST_HSC_old_plate3_biol_rep_TCL_G11
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_104

description:
ST_HSC_old_plate3_biol_rep_TCL_C11
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_94

description:
ST_HSC_old_plate2_biol_rep_TCL_E04
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_120

description:
young_ST_HSC_120
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_93

description:
ST_HSC_old_plate2_biol_rep_TCL_B04
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_91

description:
ST_HSC_old_plate2_biol_rep_TCL_D04
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_96

description:
ST_HSC_old_plate2_biol_rep_TCL_C04
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_98

description:
ST_HSC_old_plate3_biol_rep_TCL_F11
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_95

description:
ST_HSC_old_plate2_biol_rep_TCL_A04
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_114

description:
young_ST_HSC_114
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_116

description:
young_ST_HSC_116
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_118

description:
young_ST_HSC_118
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_117

description:
young_ST_HSC_117
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_89

description:
ST_HSC_old_plate2_biol_rep_TCL_G04
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_115

description:
young_ST_HSC_115
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_88

description:
ST_HSC_old_plate2_biol_rep_TCL_C06
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_90

description:
ST_HSC_old_plate2_biol_rep_TCL_F04
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_109

description:
young_ST_HSC_109
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_107

description:
young_ST_HSC_107
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_108

description:
young_ST_HSC_108
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_87

description:
ST_HSC_old_plate2_biol_rep_TCL_A06
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_86

description:
ST_HSC_old_plate2_biol_rep_TCL_E06
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_92

description:
ST_HSC_old_plate2_biol_rep_TCL_H04
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_113

description:
young_ST_HSC_113
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_110

description:
young_ST_HSC_110
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_106

description:
young_ST_HSC_106
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_111

description:
young_ST_HSC_111
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_112

description:
young_ST_HSC_112
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_84

description:
ST_HSC_old_plate2_biol_rep_TCL_H06
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_105

description:
young_ST_HSC_105
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_81

description:
ST_HSC_old_plate2_biol_rep_TCL_G06
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_104

description:
young_ST_HSC_104
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_82

description:
ST_HSC_old_plate2_biol_rep_TCL_F06
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_80

description:
ST_HSC_old_plate2_biol_rep_TCL_C01
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_83

description:
ST_HSC_old_plate2_biol_rep_TCL_D06
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_102

description:
young_ST_HSC_102
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_101

description:
young_ST_HSC_101
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_99

description:
young_ST_HSC_99
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_79

description:
ST_HSC_old_plate2_biol_rep_TCL_A01
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_103

description:
young_ST_HSC_103
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_78

description:
ST_HSC_old_plate2_biol_rep_TCL_E01
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_98

description:
young_ST_HSC_98
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_77

description:
ST_HSC_old_plate2_biol_rep_TCL_B01
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_96

description:
young_ST_HSC_96
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_97

description:
young_ST_HSC_97
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_76

description:
ST_HSC_old_plate2_biol_rep_TCL_H01
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_95

description:
young_ST_HSC_95
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_94

description:
young_ST_HSC_94
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_73

description:
ST_HSC_old_plate2_biol_rep_TCL_G01
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_71

description:
ST_HSC_old_plate2_biol_rep_TCL_A12
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_74

description:
ST_HSC_old_plate2_biol_rep_TCL_F01
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_70

description:
ST_HSC_old_plate2_biol_rep_TCL_E12
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_69

description:
ST_HSC_old_plate2_biol_rep_TCL_B12
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_100

description:
young_ST_HSC_100
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_93

description:
young_ST_HSC_93
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_91

description:
young_ST_HSC_91
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_92

description:
young_ST_HSC_92
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_90

description:
young_ST_HSC_90
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_67

description:
ST_HSC_old_plate2_biol_rep_TCL_D12
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_68

description:
ST_HSC_old_plate2_biol_rep_TCL_H12
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_66

description:
ST_HSC_old_plate2_biol_rep_TCL_F12
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_88

description:
young_ST_HSC_88
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_65

description:
ST_HSC_old_plate2_biol_rep_TCL_G12
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_75

description:
ST_HSC_old_plate2_biol_rep_TCL_D01
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_63

description:
ST_HSC_old_plate2_biol_rep_TCL_A05
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_87

description:
young_ST_HSC_87
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_89

description:
young_ST_HSC_89
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_62

description:
ST_HSC_old_plate2_biol_rep_TCL_E05
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_64

description:
ST_HSC_old_plate2_biol_rep_TCL_C05
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_86

description:
young_ST_HSC_86
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_85

description:
young_ST_HSC_85
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_61

description:
ST_HSC_old_plate2_biol_rep_TCL_B05
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_72

description:
ST_HSC_old_plate2_biol_rep_TCL_C12
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_83

description:
young_ST_HSC_83
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_81

description:
young_ST_HSC_81
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_58

description:
ST_HSC_old_plate2_biol_rep_TCL_F05
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_80

description:
young_ST_HSC_80
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_59

description:
ST_HSC_old_plate2_biol_rep_TCL_D05
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_57

description:
ST_HSC_old_plate2_biol_rep_TCL_G05
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_79

description:
young_ST_HSC_79
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_78

description:
young_ST_HSC_78
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_77

description:
young_ST_HSC_77
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_55

description:
ST_HSC_old_plate2_biol_rep_TCL_A09
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_54

description:
ST_HSC_old_plate2_biol_rep_TCL_E09
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_56

description:
ST_HSC_old_plate2_biol_rep_TCL_C09
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_84

description:
young_ST_HSC_84
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_75

description:
young_ST_HSC_75
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_74

description:
young_ST_HSC_74
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_76

description:
young_ST_HSC_76
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_53

description:
ST_HSC_old_plate2_biol_rep_TCL_B09
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_52

description:
ST_HSC_old_plate2_biol_rep_TCL_H09
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_73

description:
young_ST_HSC_73
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_51

description:
ST_HSC_old_plate2_biol_rep_TCL_D09
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_50

description:
ST_HSC_old_plate2_biol_rep_TCL_F09
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_60

description:
ST_HSC_old_plate2_biol_rep_TCL_H05
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_71

description:
young_ST_HSC_71
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_48

description:
ST_HSC_old_plate2_biol_rep_TCL_C07
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_72

description:
young_ST_HSC_72
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_47

description:
ST_HSC_old_plate2_biol_rep_TCL_A07
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_82

description:
young_ST_HSC_82
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_68

description:
young_ST_HSC_68
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_69

description:
young_ST_HSC_69
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_70

description:
young_ST_HSC_70
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_46

description:
ST_HSC_old_plate2_biol_rep_TCL_E07
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_45

description:
ST_HSC_old_plate2_biol_rep_TCL_B07
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_67

description:
young_ST_HSC_67
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_66

description:
young_ST_HSC_66
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_43

description:
ST_HSC_old_plate2_biol_rep_TCL_D07
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_64

description:
young_ST_HSC_64
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_49

description:
ST_HSC_old_plate2_biol_rep_TCL_G09
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_41

description:
ST_HSC_old_plate2_biol_rep_TCL_G07
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_62

description:
young_ST_HSC_62
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_40

description:
ST_HSC_old_plate2_biol_rep_TCL_C02
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_60

description:
young_ST_HSC_60
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_61

description:
young_ST_HSC_61
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_37

description:
ST_HSC_old_plate2_biol_rep_TCL_B02
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_38

description:
ST_HSC_old_plate2_biol_rep_TCL_E02
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_59

description:
young_ST_HSC_59
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_39

description:
ST_HSC_old_plate2_biol_rep_TCL_A02
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_36

description:
ST_HSC_old_plate2_biol_rep_TCL_H02
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_58

description:
young_ST_HSC_58
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_56

description:
young_ST_HSC_56
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_57

description:
young_ST_HSC_57
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_34

description:
ST_HSC_old_plate2_biol_rep_TCL_F02
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_32

description:
ST_HSC_old_plate2_biol_rep_TCL_C10
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_55

description:
young_ST_HSC_55
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_54

description:
young_ST_HSC_54
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_53

description:
young_ST_HSC_53
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_42

description:
ST_HSC_old_plate2_biol_rep_TCL_F07
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_33

description:
ST_HSC_old_plate2_biol_rep_TCL_G02
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_44

description:
ST_HSC_old_plate2_biol_rep_TCL_H07
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_52

description:
young_ST_HSC_52
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_63

description:
young_ST_HSC_63
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_65

description:
young_ST_HSC_65
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_51

description:
young_ST_HSC_51
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_31

description:
ST_HSC_old_plate2_biol_rep_TCL_A10
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_30

description:
ST_HSC_old_plate2_biol_rep_TCL_E10
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_29

description:
ST_HSC_old_plate2_biol_rep_TCL_B10
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_50

description:
young_ST_HSC_50
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_25

description:
ST_HSC_old_plate2_biol_rep_TCL_G10
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_28

description:
ST_HSC_old_plate2_biol_rep_TCL_H10
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_27

description:
ST_HSC_old_plate2_biol_rep_TCL_D10
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_24

description:
ST_HSC_old_plate2_biol_rep_TCL_C08
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_35

description:
ST_HSC_old_plate2_biol_rep_TCL_D02
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_44

description:
young_ST_HSC_44
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_45

description:
young_ST_HSC_45
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_47

description:
young_ST_HSC_47
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_26

description:
ST_HSC_old_plate2_biol_rep_TCL_F10
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_46

description:
young_ST_HSC_46
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_22

description:
ST_HSC_old_plate2_biol_rep_TCL_E08
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_23

description:
ST_HSC_old_plate2_biol_rep_TCL_A08
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_43

description:
young_ST_HSC_43
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_20

description:
ST_HSC_old_plate2_biol_rep_TCL_H08
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_19

description:
ST_HSC_old_plate2_biol_rep_TCL_D08
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_21

description:
ST_HSC_old_plate2_biol_rep_TCL_B08
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_49

description:
young_ST_HSC_49
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_40

description:
young_ST_HSC_40
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_18

description:
ST_HSC_old_plate2_biol_rep_TCL_F08
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_48

description:
young_ST_HSC_48
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_17

description:
ST_HSC_old_plate2_biol_rep_TCL_G08
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_37

description:
young_ST_HSC_37
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_16

description:
ST_HSC_old_plate2_biol_rep_TCL_C03
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_41

description:
young_ST_HSC_41
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_38

description:
young_ST_HSC_38
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_42

description:
young_ST_HSC_42
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_36

description:
young_ST_HSC_36
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_15

description:
ST_HSC_old_plate2_biol_rep_TCL_A03
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_39

description:
young_ST_HSC_39
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_35

description:
young_ST_HSC_35
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_13

description:
ST_HSC_old_plate2_biol_rep_TCL_B03
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_34

description:
young_ST_HSC_34
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_14

description:
ST_HSC_old_plate2_biol_rep_TCL_E03
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_33

description:
young_ST_HSC_33
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_11

description:
ST_HSC_old_plate2_biol_rep_TCL_D03
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_12

description:
ST_HSC_old_plate2_biol_rep_TCL_H03
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_32

description:
young_ST_HSC_32
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_9

description:
ST_HSC_old_plate2_biol_rep_TCL_G03
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_30

description:
young_ST_HSC_30
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_8

description:
ST_HSC_old_plate2_biol_rep_TCL_C11
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_29

description:
young_ST_HSC_29
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_7

description:
ST_HSC_old_plate2_biol_rep_TCL_A11
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_85

description:
ST_HSC_old_plate2_biol_rep_TCL_B06
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_27

description:
young_ST_HSC_27
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_28

description:
young_ST_HSC_28
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_26

description:
young_ST_HSC_26
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_5

description:
ST_HSC_old_plate2_biol_rep_TCL_B11
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_6

description:
ST_HSC_old_plate2_biol_rep_TCL_E11
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_4

description:
ST_HSC_old_plate2_biol_rep_TCL_H11
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_25

description:
young_ST_HSC_25
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_2

description:
ST_HSC_old_plate2_biol_rep_TCL_F11
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_1

description:
ST_HSC_old_plate2_biol_rep_TCL_G11
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_24

description:
young_ST_HSC_24
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_3

description:
ST_HSC_old_plate2_biol_rep_TCL_D11
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_23

description:
young_ST_HSC_23
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_22

description:
young_ST_HSC_22
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_190

description:
LT_HSC_old_plate3_biol_rep_TCL_E4
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx

value: long term hematopoietic stem cell


title: young_C57BL6_STHSC_31

description:
young_ST_HSC_31
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_19

description:
young_ST_HSC_19
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_18

description:
young_ST_HSC_18
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_21

description:
young_ST_HSC_21
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_192

description:
LT_HSC_old_plate3_biol_rep_TCL_C4
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_187

description:
LT_HSC_old_plate3_biol_rep_TCL_D4
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_16

description:
young_ST_HSC_16
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_20

description:
young_ST_HSC_20
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_191

description:
LT_HSC_old_plate3_biol_rep_TCL_A4
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_17

description:
young_ST_HSC_17
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_186

description:
LT_HSC_old_plate3_biol_rep_TCL_F4
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_STHSC_biol_replicate_10

description:
ST_HSC_old_plate2_biol_rep_TCL_F03
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_189

description:
LT_HSC_old_plate3_biol_rep_TCL_B4
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_185

description:
LT_HSC_old_plate3_biol_rep_TCL_G4
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_14

description:
young_ST_HSC_14
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_188

description:
LT_HSC_old_plate3_biol_rep_TCL_H4
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_183

description:
LT_HSC_old_plate3_biol_rep_TCL_A6
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_13

description:
young_ST_HSC_13
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_15

description:
young_ST_HSC_15
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_11

description:
young_ST_HSC_11
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_10

description:
young_ST_HSC_10
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_182

description:
LT_HSC_old_plate3_biol_rep_TCL_E6
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_12

description:
young_ST_HSC_12
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_9

description:
young_ST_HSC_9
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_180

description:
LT_HSC_old_plate3_biol_rep_TCL_H6
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_181

description:
LT_HSC_old_plate3_biol_rep_TCL_B6
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_179

description:
LT_HSC_old_plate3_biol_rep_TCL_D6
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_8

description:
young_ST_HSC_8
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_6

description:
young_ST_HSC_6
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_184

description:
LT_HSC_old_plate3_biol_rep_TCL_C6
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_7

description:
young_ST_HSC_7
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_177

description:
LT_HSC_old_plate3_biol_rep_TCL_G6
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_5

description:
young_ST_HSC_5
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_175

description:
LT_HSC_old_plate3_biol_rep_TCL_A1
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_3

description:
young_ST_HSC_3
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_176

description:
LT_HSC_old_plate3_biol_rep_TCL_C1
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_4

description:
young_ST_HSC_4
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_173

description:
LT_HSC_old_plate3_biol_rep_TCL_B1
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_1

description:
young_ST_HSC_1
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_171

description:
LT_HSC_old_plate3_biol_rep_TCL_D1
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_192

description:
young_LT_HSC_192
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_190

description:
young_LT_HSC_190
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_168

description:
LT_HSC_old_plate3_biol_rep_TCL_C12
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_172

description:
LT_HSC_old_plate3_biol_rep_TCL_H1
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_167

description:
LT_HSC_old_plate3_biol_rep_TCL_A12
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_STHSC_2

description:
young_ST_HSC_2
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_189

description:
young_LT_HSC_189
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_170

description:
LT_HSC_old_plate3_biol_rep_TCL_F1
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_178

description:
LT_HSC_old_plate3_biol_rep_TCL_F6
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_169

description:
LT_HSC_old_plate3_biol_rep_TCL_G1
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_191

description:
young_LT_HSC_191
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_165

description:
LT_HSC_old_plate3_biol_rep_TCL_B12
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_186

description:
young_LT_HSC_186
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_187

description:
young_LT_HSC_187
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_164

description:
LT_HSC_old_plate3_biol_rep_TCL_H12
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_185

description:
young_LT_HSC_185
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_163

description:
LT_HSC_old_plate3_biol_rep_TCL_D12
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_184

description:
young_LT_HSC_184
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_162

description:
LT_HSC_old_plate3_biol_rep_TCL_F12
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_188

description:
young_LT_HSC_188
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_161

description:
LT_HSC_old_plate3_biol_rep_TCL_G12
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_183

description:
young_LT_HSC_183
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_174

description:
LT_HSC_old_plate3_biol_rep_TCL_E1
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_182

description:
young_LT_HSC_182
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_160

description:
LT_HSC_old_plate3_biol_rep_TCL_C5
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_180

description:
young_LT_HSC_180
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: polycysticstemcell_expansionmedium_1 16p6

city: The Hague

first: Edgar

last: Wills

address: Andrej Sacharowstraat 37

department: Gastroenterology & Hepatology

country: Netherlands

institute: RadboudUMC

data_processing:
Custom programs were used to demultiplex individual samples using their unique index. We used CLC Genomics Workbench 7 for RNAseq alignment and statistical analysis of the data. Human HG19 was used as reference sequence.
To calculate gene expression intensity, read counts were normalized to reads. Methods for data normalization and analysis are based on the use of “internal standards” (Rocke DM, Durbin B. A model for measurement error for gene expression arrays. J Comput Biol 2001;8:557-69.) that characterize some aspects of the system’s behavior, such as technical variability, as presented elsewhere (Dozmorov I, Centola M. An associative analysis of gene expression array data. Bioinformatics 2003;19:204-11.;Dozmorov I, Lefkovits I. Internal standard-based analysis of microarray data. Part 1: analysis of differential gene expressions. Nucleic Acids Res 2009;37:6323-39). The comparison of these methods with some other normalization and analysis procedures was presented in 28. Created initially for the analysis of microarray data they were slightly modified to the needs of RNAseq data analysis.
The two-step normalization procedure and the Associative analysis functions are implemented in MatLab (Mathworks; MA, USA) and available from authors upon request. These algorithms are also obtainable from an R package diffGeneAnalysis, available as a part of Bioconductor packages (http://www.bioconductor.org/packages/2.5/bioc/html/diffGeneAnalysis.html).
Genome_build: HG19
Supplementary_files_format_and_content: tab-delimited text files include RPKM values for each Sample ...

molecule: polyA RNA

organism: Homo sapiens

source_name: biliary stem cell, EM, polycystic

growth_protocol: human adult liver stem cell expansion medium (Huch et al, Cell 2015)

extract_protocol:
RNA from organoids was isolated using the NucleoSpin® RNA kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s protocol. Quality control was performed with Agilent Bioanalyser.
polyA mRNA selection, cDNA synthesis, PCR enrichment (Illumina TruSeq mRNA Sample Preparation kit according to the manufacturer’s protocol (Cat#FC-122-1001) )

treatment_protocol: Organoids were removed from medium, mechanically dissociated before RNA isolation.

tag: gender

value: female

value: Polycystic biliary stem cells

tag: source

value: cyst epithelium

tag: disease state

value: ADPLD

tag: mutation

value: unknown

tag: passage number


title: old_C57BL6_LTHSC_biol_replicate_158

description:
LT_HSC_old_plate3_biol_rep_TCL_E5
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_181

description:
young_LT_HSC_181
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx

value: 2-3 months old


title: polycysticstemcell_expansionmedium_1 15p6

value: cyst fluid

value: ADPKD

value: Undetermined


title: old_C57BL6_LTHSC_biol_replicate_166

description:
LT_HSC_old_plate3_biol_rep_TCL_E12
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx

value: 22 months old


title: young_C57BL6_LTHSC_179

description:
young_LT_HSC_179
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: polycysticstemcell_expansionmedium_1 12p5


title: old_C57BL6_LTHSC_biol_replicate_157

description:
LT_HSC_old_plate3_biol_rep_TCL_B5
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_178

description:
young_LT_HSC_178
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: polycysticstemcell_expansionmedium_1 10p4


title: old_C57BL6_LTHSC_biol_replicate_156

description:
LT_HSC_old_plate3_biol_rep_TCL_H5
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: polycysticstemcell_expansionmedium_1 9p6

value: PRKCSH 1362-2A>G


title: old_C57BL6_LTHSC_biol_replicate_154

description:
LT_HSC_old_plate3_biol_rep_TCL_F5
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: polycysticstemcell_expansionmedium_1 8p6


title: young_C57BL6_LTHSC_175

description:
young_LT_HSC_175
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: polycysticstemcell_expansionmedium_1 2p6

value: solitary cyst


title: old_C57BL6_LTHSC_biol_replicate_153

description:
LT_HSC_old_plate3_biol_rep_TCL_G5
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: polycysticstemcell_expansionmedium_1 17p6


title: normalbiliarystemcell_expansionmedium_1 n10

source_name: biliary stem cell, EM, normal

value: male

value: Normal biliary stem cells

value: liver parenchyma

value: control (clean resection edge liver metastasis)


title: young_C57BL6_LTHSC_173

description:
young_LT_HSC_173
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx

value: long term hematopoietic stem cell


title: old_C57BL6_LTHSC_biol_replicate_159

description:
LT_HSC_old_plate3_biol_rep_TCL_A5
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_151

description:
LT_HSC_old_plate3_biol_rep_TCL_A9
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: normalbiliarystemcell_expansionmedium_1 n4


title: young_C57BL6_LTHSC_177

description:
young_LT_HSC_177
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_172

description:
young_LT_HSC_172
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: normalbiliarystemcell_expansionmedium_1 n3


title: normalbiliarystemcell_expansionmedium_1 n2


title: young_C57BL6_LTHSC_170

description:
young_LT_HSC_170
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_148

description:
LT_HSC_old_plate3_biol_rep_TCL_H9
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_150

description:
LT_HSC_old_plate3_biol_rep_TCL_E9
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_147

description:
LT_HSC_old_plate3_biol_rep_TCL_D9
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_168

description:
young_LT_HSC_168
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_171

description:
young_LT_HSC_171
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: hES_OPCs

city: Cleveland

first: Tyler

middle: E

last: Miller

state: Ohio

department: Stem Cell Biology and Regenerative Medicine

institute: Cleveland Clinic Lerner Research Institute

description: PolyA+ RNA

data_processing:
For gene expression analysis, reads were aligned to the hg19 genome build (retrieved from http://cufflinks.cbcb.umd.edu/igenomes.html), using Tophat v2.0.6 (Trapnell et  al., 2009).
The distribution of alignments was analyzed using the CollectRnaSeqMetrics module of Picard v1.89 (http://picard.sourceforge.net/).
FPKM values for known genes were calculated using Cufflinks v2.0.2 (Trapnell et al., 2010) provided with the GTF file via the -G (known genes only) option. FPKM values were tabled by converting background values (<0.25) to 0 and adding 0.25 to all values (Ramskold et al., 2009).
Genome_build: Hg19
Supplementary_files_format_and_content: FPKM values from Cufflinks

source_name: human Embryonic Stem Cell-derived Oligodendrocyte progenitor cells

extract_protocol:
RNA was extracted from ~2x106 cells with TRIzol (Invitrogen), separated using Phase Lock Gel tubes (5 Prime), and purified using the miRNAeasy kit (Qiagen) according to the manufacturer’s protocol.
PolyA+ RNA was prepared for sequencing using the Illumina TruSeq RNA Sample Preparation Kit according to the manufacturer's protocol.
RNA-seq libraries were sequenced on the Illumina HiSeq 2500 platform at the Case Western Reserve University Genomics Core Facility.

tag: growth protocol

value: Human OPCs were differentiated from the human embryonic stem cell line H7 over a 154 day protocol (NIH Human Embryonic Stem Cell Registry WA07; NIH Approval Number:NIHhESC-10-0061) as previously demonstrated (Hu et al., 2009; Wang et al., 2013).

tag: cell characterization

value: hESC-derived OPCs were characterized by co-staining of Sox10 (R&D Systems,AF2864; 1:100) and Olig2 (Millipore,AB9610 1:500).


title: p0-IN528_GBM-CSCs

description: PolyA+ RNA

source_name: Primary Patient Derived Tumor Model

value: IN528 cells from a primary patient derived GBM tumor model were injected subcutaneously into the flank of a immunocomprimised athymic nude mouse and allowed to grow into a tumor.

value: Cells were dissociated from the tumor and sorted on CD133 to collect the cancer stem cell population.


title: old_C57BL6_LTHSC_biol_replicate_149

description:
LT_HSC_old_plate3_biol_rep_TCL_B9
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_155

description:
LT_HSC_old_plate3_biol_rep_TCL_D5
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_176

description:
young_LT_HSC_176
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: Glial_Progenitors

description: PolyA+ RNA

source_name: Primary Epilepsy tissued derived progenitor cells

value: Normal, non-neoplastic cells were derived from patient tissue specimens of neurosurgical resection in accordance with a Cleveland Clinic Institutional Review Board-approved protocol. Informed consent was obtained by the tissue bank, which provided de-identified excess tissue to the laboratory immediately following surgical resection. Specimens used for cell culture were dissociated with a Papain dissociation kit (Worthington). Cells were cultured adherently in media containing 50% Neurobasal medium (Gibco) and 50% Dulbecco's modified Eagle medium (DMEM) with B27 (without vitamin A, Invitrogen, basic fibroblast growth factor (10 ng/ml), epidermal growth factor (10 ng/ml), sodium pyruvate and L-glutamine, and 5% FBS. All cultured cells were used within five passages of dissociation.

value: Single cells were sorted using anti-A2B5 MicroBeads (130-093-388, Miltenyi Biotec) according to manufacturer’s protocol. In brief, live cells were incubated first with FcR Blocking Reagent (Miltenyi) for 10 mins then A2B5 antibody for 15 min at 4oC (2.5 μg A2B5 antibody per million cells). Cells positive for A2B5 were double enriched by passing through magnetic field (MACS Separator) twice. Both A2B5 positive and negative fractions were collected, lysed with TRIzol (Invitrogen) for RNA isolation. A population of A2B5+ cells were plated and allowed to attach overnight before fixing and staining with standard immunofluorescence techniques. A2B5+ cells stained positive for GFAP (DAKO, 1:5000) and Nestin (DSHB, 1:200).


title: young_C57BL6_LTHSC_174

description:
young_LT_HSC_174
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx

value: 2-3 months old


title: young_C57BL6_LTHSC_167

description:
young_LT_HSC_167
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_152

description:
LT_HSC_old_plate3_biol_rep_TCL_C9
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_146

description:
LT_HSC_old_plate3_biol_rep_TCL_F9
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: GBM-CSCs_H3K4me1

library_source: genomic

data_processing:
The FASTX-Toolkit (http://hannonlab.cshl.edu/fastx_toolkit/) was used to remove adapter sequences and trim read ends using a quality score cutoff of 20.
ChIP-seq data were aligned to hg19 genome assemblies (retrieved from http://cufflinks.cbcb.umd.edu/igenomes.html), using Bowtie v0.12.9 (Langmead et al., 2009), allowing reads with ≤ 2 mismatches and discarding reads with > 1 reportable alignment ("-m 1" parameter).
PCR duplicates were removed using SAMtools (Li et al., 200 9).
Peaks were detected with MACS v1.4 (Zhang et al., 2008), using an aligned input DNA sample as control.
Genome_build: Hg19
Supplementary_files_format_and_content: Wiggle tracks stepped at 25 bp were generated by MACS, normalized to the median signal by chromosome and visualized on the UCSC Genome Browser.

molecule: genomic DNA

growth_protocol: IN528 cells from a primary patient derived GBM tumor model were injected subcutaneously into the flank of a immunocomprimised athymic nude mouse and allowed to grow into a tumor. Cells were dissociated from the tumor and sorted on CD133 to collect the cancer stem cell population.

extract_protocol:
H3K4me1ChIP was performed from 5x106 crosslinked p0-CSCs and DNA isolated as previously described  (Rabbit anti-H3K4me1 Abcam #8895) (Corradin et al., 2014).
Sequencing libraries were prepared as previously described (Corradin et al. 2014).
ChIP-seq. ChIP-seq libraries were sequenced on the Illumina HiSeq 2500 platform at the Case Western Reserve University Genomics Core Facility.

tag: tumor model

tag: antibody

value: Rabbit anti-H3K4me1 Abcam #8895


title: old_C57BL6_LTHSC_biol_replicate_145

description:
LT_HSC_old_plate3_biol_rep_TCL_G9
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_166

description:
young_LT_HSC_166
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: KO_CITCO 2

city: Baltimore

first: Hongbing

last: Wang

address: 20 Penn Street

institute: University of Maryland, Baltimore

description:
10022051.1KO_CITCO%KO_DMSO.cpm
KO_CITCOvsKO_DMSO.geneid
KO_CITCOvsWT_CITCO.geneid

data_processing:
Reads were generated by Illumina Genome Analyzer and HiSeq instruments and delivered in the Illumina1.8+ format and Phred scaled
Reads were trimmed if needed using Flexbar version 2.4, and mapping was performed on human genome reference sequence GRCh38 (Ensembl annotation) using Tophat version 2.0.10 in conjunction with Bowtie version 1.0.0
Cufflinks version 2.1.1 was used to detect expressed genes by comparing Tophat2 read mapping with the Ensembl annotation of grch37 human genome before Cuffdiff version 2.1.1 was used to generate FKPM expression values
The EdgeR R Bioconductor 2.12 package was used with the easyRNASeq package to generate the per sample counts per million matrix which was analyzed for differential expression using a general linearized model to account for multiple experimental factors according to the EdgeR User's Guide.
Genome_build: GRCh38
Supplementary_files_format_and_content: 10022051.1KO_CITCO%KO_DMSO.cpm: Per sample counts per million
Supplementary_files_format_and_content: KO_CITCOvsKO_DMSO.geneid: Differentially-expressed genes in the KO_CITCO group compared with the KO_DMSO group
Supplementary_files_format_and_content: KO_CITCOvsWT_CITCO.geneid: Differentially-expressed genes in the KO_CITCO group normalized to the KO_DMSO group compared with the WT_CITCO group normalized to the WT_DMSO group
Supplementary_files_format_and_content: KO_DMSOvsWT_DMSO.geneid: Differentially-expressed genes in the KO_DMSO group compared with the WT_DMSO group
Supplementary_files_format_and_content: KO_PBvsKO_DMSO.geneid: Differentially-expressed genes in the KO_PB group compared with the KO_DMSO group
Supplementary_files_format_and_content: KO_PBvsWT_PB.geneid: Differentially-expressed genes in the KO_PB group normalized to KO_DMSO as compared with the WT_PB group normalized to WT_DMSO
Supplementary_files_format_and_content: WT_CITCOvsWT_DMSO.geneid: Differentially-expressed genes in the WT_CITCO group compared with the WT_DMSO group
Supplementary_files_format_and_content: WT_PBvsWT_DMSO.geneid: Differentially-expressed genes in the WT_PB group compared with the WT_DMSO group

source_name: hepatoma cell line

extract_protocol:
After drug treatment, RNA samples from treated WT and hCAR-KO cells were isolated using the miRNeasy Mini Kit (Qiagen, Valencia, CA) following the manufacturer’s instruction.
RNA libraries were prepared for sequencing using standard Illumina protocols

tag: cell line

value: HepaRG

tag: genotype/variation

value: hCAR-KO

tag: agent

value: CITCO


title: KO_CITCO 1


title: young_C57BL6_LTHSC_164

description:
young_LT_HSC_164
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_143

description:
LT_HSC_old_plate3_biol_rep_TCL_A7
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx

value: 22 months old


title: old_C57BL6_LTHSC_biol_replicate_144

description:
LT_HSC_old_plate3_biol_rep_TCL_C7
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_165

description:
young_LT_HSC_165
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: KO_PB 2

description:
10022051.1KO_CITCO%KO_DMSO.cpm
KO_PBvsKO_DMSO.geneid
KO_PBvsWT_PB.geneid

value: PB


title: young_C57BL6_LTHSC_169

description:
young_LT_HSC_169
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_141

description:
LT_HSC_old_plate3_biol_rep_TCL_B7
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: KO_PB 1


title: young_C57BL6_LTHSC_161

description:
young_LT_HSC_161
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_163

description:
young_LT_HSC_163
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_162

description:
young_LT_HSC_162
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_139

description:
LT_HSC_old_plate3_biol_rep_TCL_D7
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_140

description:
LT_HSC_old_plate3_biol_rep_TCL_H7
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: KO_DMSO 2

description:
10022051.1KO_CITCO%KO_DMSO.cpm
KO_CITCOvsKO_DMSO.geneid
KO_DMSOvsWT_DMSO.geneid
KO_PBvsKO_DMSO.geneid

value: DMSO


title: young_C57BL6_LTHSC_160

description:
young_LT_HSC_160
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: WT_CITCO 2

description:
10022051.1KO_CITCO%KO_DMSO.cpm
WT_CITCOvsWT_DMSO.geneid
KO_CITCOvsWT_CITCO.geneid

value: WT


title: old_C57BL6_LTHSC_biol_replicate_138

description:
LT_HSC_old_plate3_biol_rep_TCL_F7
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: WT_CITCO 1


title: young_C57BL6_LTHSC_158

description:
young_LT_HSC_158
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_137

description:
LT_HSC_old_plate3_biol_rep_TCL_G7
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: WT_PB 2

description:
10022051.1KO_CITCO%KO_DMSO.cpm
WT_PBvsWT_DMSO.geneid
KO_PBvsWT_PB.geneid


title: old_C57BL6_LTHSC_biol_replicate_136

description:
LT_HSC_old_plate3_biol_rep_TCL_C2
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_159

description:
young_LT_HSC_159
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_157

description:
young_LT_HSC_157
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: WT_PB 1


title: young_C57BL6_LTHSC_156

description:
young_LT_HSC_156
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_134

description:
LT_HSC_old_plate3_biol_rep_TCL_E2
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_155

description:
young_LT_HSC_155
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_142

description:
LT_HSC_old_plate3_biol_rep_TCL_E7
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: WT_DMSO 2

description:
10022051.1KO_CITCO%KO_DMSO.cpm
WT_CITCOvsWT_DMSO.geneid
KO_DMSOvsWT_DMSO.geneid
WT_PBvsWT_DMSO.geneid


title: WT_DMSO 1


title: old_C57BL6_LTHSC_biol_replicate_133

description:
LT_HSC_old_plate3_biol_rep_TCL_B2
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_154

description:
young_LT_HSC_154
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: KO_DMSO 1

description:
10022051.1KO_CITCO%KO_DMSO.cpm
KO_CITCOvsKO_DMSO.geneid
KO_DMSOvsWT_DMSO.geneid
KO_PBvsKO_DMSO.geneid


title: old_C57BL6_LTHSC_biol_replicate_132

description:
LT_HSC_old_plate3_biol_rep_TCL_H2
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_153

description:
young_LT_HSC_153
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_131

description:
LT_HSC_old_plate3_biol_rep_TCL_D2
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: mlx1 HSD 8h repB

data_processing:
Alignment to reference genome was done with BWA v.0.5.9 with default parameters.
Reads with MAPQ >= 10 were selected to further processing with Samtools.
Exon level read counts were processed with HTSeq 0.6.1 using MAPQ >= 10 reads.
Genome_build: FlyBase Drosophila melanogaster r5.54
Supplementary_files_format_and_content: tab-delimined txt file contains read counts for each sample

source_name: Mlx null mutant 3rd instar high sugar diet

growth_protocol: Control and mlx1 mutant flies were kept in egg laying champers with apple juice plates and yeast paste for 3 hours. 24 hours later the 1st instar larvae were transferred to 10% yeast plates. 24 hours later larvae were transferred to either a 10% yeast + 20% sucrose or a fresh 10% yeast plate. Non-feeding larvae were removed after 1.5 hours. Low sugar samples were collected at the 1.5h time point and high sugar samples were collected 8 hours after the transfer.

extract_protocol:
Larvae were frozen in liquid nitrogen and homogenized with a disposable plastic pestle in lysis buffer and vortexed. Samples were frozen for complete lysis of the tissue. Total RNA was extracted with Nucleospin RNA II kit according to manufacturer's protocol. Elution was done with water and the samples stored at -80°C.
Library contruction was performed at BGI with the following protocol: the total RNA samples were first treated with Dnase I. Then the mRNA was enriched by using the oligo(dT) magnetic beads. Mixed with the fragmentation buffer, the mRNA was fragmented into short fragments. The first stand of cDNA was synthesized by using random hexamer-primer. Buffer, dNTPs, RNase H and DNA polymerase I were added to synthesize the second strand. The double strand cDNA was purified with magnetic beads. End reparation and 3'-end single nucleotide A addition was performed. Finally, sequencing adaptors were ligated to the fragments and fragment enrichment was done by PCR amplification.

tag: genotype

value: Mlx -/-

tag: tissue

value: whole larvae

value: 3rd instar


title: mlx1 HSD 8h repC

tag: developmental stage


title: young_C57BL6_LTHSC_151

description:
young_LT_HSC_151
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx

tag: strain

tag: age

value: long term hematopoietic stem cell


title: young_C57BL6_LTHSC_152

description:
young_LT_HSC_152
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx

tag: cell type


title: old_C57BL6_LTHSC_biol_replicate_130

description:
LT_HSC_old_plate3_biol_rep_TCL_F2
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: mlx1 HSD 8h repA


title: old_C57BL6_LTHSC_biol_replicate_129

description:
LT_HSC_old_plate3_biol_rep_TCL_G2
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_150

description:
young_LT_HSC_150
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: control HSD 8h repB

source_name: wild-type 3rd instar high sugar diet

value: wild type


title: control HSD 8h repA


title: old_C57BL6_LTHSC_biol_replicate_128

description:
LT_HSC_old_plate3_biol_rep_TCL_C10
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_135

description:
LT_HSC_old_plate3_biol_rep_TCL_A2
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_126

description:
LT_HSC_old_plate3_biol_rep_TCL_E10
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: mlx1 LSD 1.5h repC

source_name: Mlx null mutant 3rd instar control diet


title: mlx1 LSD 1.5h repB


title: young_C57BL6_LTHSC_148

description:
young_LT_HSC_148
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_146

description:
young_LT_HSC_146
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: mlx1 LSD 1.5h repA


title: young_C57BL6_LTHSC_149

description:
young_LT_HSC_149
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_147

description:
young_LT_HSC_147
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_127

description:
LT_HSC_old_plate3_biol_rep_TCL_A10
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: control LSD 1.5h repC

source_name: wild-type 3rd instar control diet


title: old_C57BL6_LTHSC_biol_replicate_124

description:
LT_HSC_old_plate3_biol_rep_TCL_H10
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_145

description:
young_LT_HSC_145
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_123

description:
LT_HSC_old_plate3_biol_rep_TCL_D10
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_144

description:
young_LT_HSC_144
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_121

description:
LT_HSC_old_plate3_biol_rep_TCL_G10
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: control LSD 1.5h repB


title: young_C57BL6_LTHSC_141

description:
young_LT_HSC_141
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: control HSD 8h repC


title: control LSD 1.5h repA


title: young_C57BL6_LTHSC_143

description:
young_LT_HSC_143
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_142

description:
young_LT_HSC_142
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_122

description:
LT_HSC_old_plate3_biol_rep_TCL_F10
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_120

description:
LT_HSC_old_plate3_biol_rep_TCL_C8
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_140

description:
young_LT_HSC_140
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_117

description:
LT_HSC_old_plate3_biol_rep_TCL_B8
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_125

description:
LT_HSC_old_plate3_biol_rep_TCL_B10
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_139

description:
young_LT_HSC_139
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_138

description:
young_LT_HSC_138
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_114

description:
LT_HSC_old_plate3_biol_rep_TCL_F8
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_116

description:
LT_HSC_old_plate3_biol_rep_TCL_H8
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_135

description:
young_LT_HSC_135
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_134

description:
young_LT_HSC_134
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_113

description:
LT_HSC_old_plate3_biol_rep_TCL_G8
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: 2_2480UCon_IL2_STAT5_i81

city: Farmington

first: Anthony

middle: T

last: Vella

state: CT

address: 263 Farmington Avenue

department: Immunology

institute: University of Connecticut Health Center

data_processing:
The 50-nt sequence reads identified by the Sequencing Service (using
Illumina’s Hi-Seq) are mapped to the genome using the BWA algorithm with default settings.
Alignment information for each read is stored in the BAM format. Only reads that pass Illumina’s purity filter, align with no more than 2 mismatches, and map uniquely to the genome are used in the subsequent analysis. In addition, unless stated otherwise, duplicate reads (“PCR duplicates”) are
Genome_build: removed. Approximately 17 million non-duplicate reads per sample were alligned to the mouse genome (mm10).

source_name: CD8 T cell

extract_protocol: Treated cells were snap frozen and shipped to ActiveMotif, Inc. who performed the STAT5A ChIP-seq analysis according to their FactorPath™ assay.

treatment_protocol: Adoptively transferred OT-I CD8 T cells were primed in vivo with SIINFEKL peptide and CD134 plus CD137 costimulatory agonists and FACS sorted from spleens 4 days later and then treated in vitro with or without 0.5 ug/ml IL-2 for 1 hour

value: effector CD8 T cell

tag: treatment

value: 0.5 ug/ml IL-2 for 1 hour

value: anti-STAT5/R & D Systems, #PA-ST5A, lot #RU07


title: old_C57BL6_LTHSC_biol_replicate_115

description:
LT_HSC_old_plate3_biol_rep_TCL_D8
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_137

description:
young_LT_HSC_137
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx

value: 2-3 months old


title: Ak3

city: omaha

first: Paras

middle: Kumar

last: Mishra

state: Nebraska

address: DRC1 Room 5047

department: Cellular and Integrative Physiology

institute: University of Nebraska medical center

data_processing:
Illumina HCS v2.05 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome using Tophat 2 aligner in basespace.
Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using cufflinks 2 and differential expression was done using cuffdiff 2.1.1 in basespace.
Genome_build: mm10
Supplementary_files_format_and_content: RPKM

source_name: Heart tissue (Akita)

extract_protocol:
RNA was isolated from fresh heart tissue using mirVana kit.
RNA libraries were prepared for sequencing using TruSeq RNA sample prepration kit from Illumina. 1μg of RNA was used to construct the library. QC was done by bioanalyzer and quantified by Qubit. 6PM strating quantity was used for further sequencing. 100 base single read run after o-botclustering was performed on Illumina HiSeq 2500.

value: Mutation in Insulin 2 gene. Type 1 diabetic model.

value: Male (12 week)

value: heart

tag: genetic background


title: 1_2480UCon_control_STAT5_i80


title: young_C57BL6_LTHSC_133

description:
young_LT_HSC_133
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_112

description:
LT_HSC_old_plate3_biol_rep_TCL_C3
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_119

description:
LT_HSC_old_plate3_biol_rep_TCL_A8
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_110

description:
LT_HSC_old_plate3_biol_rep_TCL_E3
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_118

description:
LT_HSC_old_plate3_biol_rep_TCL_E8
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: young_C57BL6_LTHSC_136

description:
young_LT_HSC_136
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_109

description:
LT_HSC_old_plate3_biol_rep_TCL_B3
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_111

description:
LT_HSC_old_plate3_biol_rep_TCL_A3
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_108

description:
LT_HSC_old_plate3_biol_rep_TCL_H3
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: old_C57BL6_LTHSC_biol_replicate_107

description:
LT_HSC_old_plate3_biol_rep_TCL_D3
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx


title: WT4

source_name: Heart tissue (Wild type)

value: Wild type


title: Ak1


title: WT3


title: young_C57BL6_LTHSC_130

description:
young_LT_HSC_130
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx

source_name: hematopoietic stem and progenitor cells


title: old_C57BL6_LTHSC_biol_replicate_106

description:
LT_HSC_old_plate3_biol_rep_TCL_F3
single cell RNA-Seq
processed data file: GEO_HSC_single_data.xlsx

value: 22 months old