title: PBSiv_NI_H3K4me3_rep3

city: Chicago

first: Luis

last: Barreiro

state: Illinois

address: 900 E 57th Street

institute: University of Chicago

data_processing:
Adapter sequences and low quality score bases (Phred score < 20) were first removed from reads.
Trimmed reads were mapped to the mouse genome reference sequence (GRCm38/mm10) and non-uniquely mapped reads and PCR duplicates were removed.
Peaks were first called on ChIP-seq samples (using respective input IPs as a control) using the MACS2 software suite (Zhang et al., 2008) with the added parameters “g mm -q 0.05 -keep-dup all”. For comparison between samples, all peaks from each sample were merged to provide one set of combined peaks. For quantification of the combined peaks, we used the bamutils count command from NGSUtils (Breese and Liu, 2013) to count the number of overlapping reads for each sample.
Genome_build: mm10
Supplementary_files_format_and_content: Tab-delimited text files include read count, CPM and RPKM values for each sample.

source_name: Bone marrow-derived macrophages

growth_protocol: Six- to ten-week old C57BL/6J, Rag1-/-, Ccr2-/-, and CD45.1+ B6 mice were purchased from Jackson Laboratories or bred at the RI-MUHC, Montreal, QC,Canada. All experiments were performed with male mice, except parabiosis, which was performed on female mice. All animal studies were conducted in accordance with the guidelines of and approved by the Animal Research Ethics Board of McGill University (project ID: 5860). Throughout the paper, mice were intravenously (iv) or subcutaneously (sc) vaccinated with 1x106 single-suspended BCG in 100µl PBS, unless otherwise indicated. Image stream analyses were used to identify what cells in the BM were infected with BCG. For the Mtb challenges, mice were either infected with ~50 CFU of Mtb H37Rv in a nose-only aerosol exposure unit (Intox), or with ~1000 CFU Mtb H37Rv via intratracheal instillation. BMDMs were derived as described previously (Coulombe et al., 2014).

extract_protocol:
DNA was extracted using the PureGene DNA extraction kit (Gentra Systems).
Samples from BCGvaccinated and non-vaccinated, Mtb-infected and non-infected BMDMs were crosslinked with 37% w/v formaldehyde for 20 minutes at RT and immediately quenched for 5 minutes with 1M Glycine at RT. The formaldehyde fixed samples were then sonicated to 150500 bp using an E220 sonicator (Covaris), and subsequently ChIP-DNA was prepared using a manual chromatin immunoprecipitation method with AntibodiesAntigen incubation for 18 hours, followed by bead incubation for 150 minutes, and 7 5minute washing steps. Approximately 1x106 cells were used for each ChIP and ~50,000 cells for the input. The following antibodies were used: H3K4me1 (Company: CST, Cat. No.: 5326P, Lot No.: 1), H3K4me3 (Thermo Fisher Scientific, MA5-11199), H3K27ac (Abcam, Ab4729, Lot No.: GR2005631). ChIP and Input libraries were prepared using the MicroPlex Library Preparation Kit v2 (C05010013, Diagenode), with alterations including: PCR enrichment (9 to 18 cycles) prior to size selection, and use of Ampure beads for size selection (250-350 bp).

treatment_protocol: BMDMs (1x10^6 cells) were seeded in 6-well plates supplemented with RPMI without penicillin/streptomycin (1 ml), and incubated overnight at 37°C with 5% CO2. Next day, cells were infected with H37Rv (MOI 1), unless otherwise indicated. Cells were incubated for 4 hours at 37°C with 5% CO2. Subsequently, cells were washed 3x with sterile PBS, and then were supplemented with RPMI without penicillin/streptomycin. CFUs were enumerated at 4 hours, day 3 and day 5 post infection.

tag: histone mark

tag: treatment

value: PBS iv

tag: infection

value: Non Infected

tag: mouse id


title: BCGiv_NI_H3K4me3_rep3

status: Public on Dec 13 2017

type: SRA

country: USA

library_source: genomic

molecule: genomic DNA

organism: Mus musculus

value: BCG iv


title: PBSiv_NI_H3K4me1_rep2


title: PBSiv_HSC_3

library_source: transcriptomic

data_processing:
Illumina bcl2fastq software used for basecalling.
Sequenced reads were trimmed for adaptor sequence and low-quality sequence, then mapped to mm10 transcriptome (ENSEMBL v 81) using STAR
Gene-level expression estimates were calculated using featureCount (Liao et al., 2014).
Genome_build: GRCm38 (mm10)
Supplementary_files_format_and_content: tab-delimited text files include count values for each Sample ...

molecule: total RNA

source_name: Hematopoietic stem cell

growth_protocol: Six to ten-week old C57BL/6J, Rag1-/, Ccr2/-, and CD45.1+ B6 mice were purchased from Jackson Laboratories or bred at the RI-MUHC, Montreal, QC, Canada. All experiments were performed with male mice, except parabiosis, which was performed on female mice. All animal studies were conducted in accordance with the guidelines of and approved by the Animal Research Ethics Board of McGill University (project ID: 5860).

extract_protocol: RNA-sequencing libraries were prepared using either the Illumina TruSeq protocol (for BMDMs) or the SMARTer® Stranded RNA-Seq Kit (for HSCs) and sequenced with single-end 100bp reads on an Illumina HiSeq2500.

treatment_protocol:
Throughout the paper, mice were intravenously (iv) or subcutaneously (sc) vaccinated with 1x106 singlesuspended BCG in 100μl PBS, unless otherwise indicated. Image stream analyses were used to identify what cells in the BM were infected with BCG. For the Mtb challenges, mice were either infected with ~50 CFU of Mtb H37Rv in a nose-only aerosol exposure unit (Intox), or with ~1000 CFU Mtb H37Rv via intratracheal instillation. BMDMs were derived as described previously (Coulombe et al., 2014) and infected with Mtb H37Rv at an MOI of 1.
C57BL/6J mice were sc or iv vaccinated with 1x106 singlesuspended BCG or iv PBSinjected. After four weeks, we generated BMDMs that were infected with H37Rv (MOI 0.5) and 0.5x106 cells were intratracheally transferred into naive Rag1-/- mice (Divangahi et al., 2009;; 2010). Parabiosis was performed as previously described (Ensan et al., 2016). Female CD45.1+ B6 mice were either PBSinjected or iv vaccinated with 1x106 singlesuspended BCGTice. After four weeks, CD45.1+ mice were joined to naive, female, weightmatched, and co-housed Ccr2-/- mice through skin suture and attachment of the front and hind legs. Six weeks post surgery the Ccr2-/- mice were intratracheally infected with H37Rv (~1000 CFU). CD45.1+ B6 mice were either PBS-injected or BCG iv-vaccinated (1x106 bacteria). After four weeks, BM cells were harvested and 4x106 cells were intravenously injected into CD45.2+ C57BL/6J mice 16 hours post irradiation with 9 Gy. Chimeric mice were aerosol infected with ~50 CFU Mtb H37Rv after 14 weeks when the hematopoietic compartment was reconstituted as determined by flow cytometry.

tag: tissue

value: Hematopoietic stem cell

tag: infection status


title: PBSiv_Mtb_input_rep1

value: Input

value: Mtb


title: PBSiv_Mtb_input_rep3


title: non-adenoma colon tissue from AOM/DSS-treated mouse

city: Columbus

first: Joanna

last: Groden

state: OH

address: 986 BRT 460 West 12th Ave.

department: Department of Cancer Biology and Genetics

institute: The Ohio State University, College of Medicine

description: mRNA-seq

data_processing:
mRNA sequencing was performed by The Ohio State University Genomics Shared Resource using a HiSeq 2500 instrument (Illumina, Inc.).
Basecall files were converted to Fastq files in BaseSpace by The Ohio State University Biomedical Informatics Shared Resource.
bam files were uploaded to Galaxy to perform alignment of reads to mm9.
Transcript expression levels were quantified as counts and in FPKM units.
Data were normalized by dividing the FPKM measurement for each adenoma by that of its corresponding adjacent non-adenoma tissue.
Genome_build: mm9
Supplementary_files_format_and_content: The file Groden_Lab_Processed_RNA-seq_data_07_07_15.xlsx is an Excel file containing normalized abundance measurements from 8 samples organized into 3 sheets.  Measurements are expressed in the first sheet as counts, and in the second sheet as Fragments Per Kilobase of transcript per Million mapped reads.  The third sheet uses FPKM measurements to calculate fold-changes in expression for each colon adenoma relative to its corresponding non-adenoma colon tissue.

source_name: colon tissue

growth_protocol: ApcMin/+ mice on a C57BL/6J background were sacrificed at 110-120 days old.  Whole mouse colons were harvested from sacrificed ApcMin/+ and AOM/DSS-treated mice and immediately stored in RNAlater solution to protect RNA quality.  Single adenomas were excised from the colons of each mouse, and an adjacent section of non-tumor colon tissue was taken as a control.

extract_protocol:
Tissues were homogenized into 500 μL each of Trizol using an OMNI TH tissue homogenizer over a period of 1-2 minutes each.  50 μg glycogen (RNase-free) was mixed into each sample.  A standard Trizol isolation protocol was then performed.  RNA was resuspended in nuclease-free water.
Eight single-read libraries were prepared using the TruSeq RNA Library Preparation Kit (Illumina catalog # RS-122-2001) according to Illumina's instructions.

treatment_protocol: Azoxymethane- and dextran sulfate sodium-treated (AOM/DSS) mice were treated as follows:  6-7 week old C57BL/6J mice were given a single intraperitoneal injection of AOM (10 mg/kg body weight).  One week later mice were treated with 2% DSS (in drinking water), followed by two weeks on water alone.  They received 3 cycles of DSS / water.  Mice were sacrificed seventy days after AOM was administered.  Whole mouse colons were harvested and immediately stored in RNAlater solution to protect RNA quality.  Single adenomas were excised from the colons of each mouse, and an adjacent section of non-tumor colon tissue was taken as a control.  Adenomas from AOM/DSS-treated mice were confirmed by Sanger sequencing to express degradation-resistant β-catenin as previously reported, indicating that they were Apc-wild-type.  The point mutations observed were all between codons 32 through 41 and consistent with previous reports.

tag: strain

tag: genotype

value: wild-type

value: AOM/DSS


title: PBSiv_NI_rep3

data_processing:
ATAC-seq reads were trimmed for adapter sequences and low quality score bases and were mapped to the mouse reference genome. Mapping was performed using BWA-MEM (Li and Durbin, 2009) in paired-end mode at default parameters. Only reads that were properly paired and had an unique alignment (mapping quality > 10) were retained. PCR duplicates were removed using Picard tools
Peaks were first called on ATAC-seq samples using the MACS2 software suite (Zhang et al., 2008) with the added parameters “g mm -q 0.05 -keep-dup all”. For comparison between samples, all peaks from each sample were merged to provide one set of combined peaks. For quantification of the combined peaks, we used the bamutils count command from NGSUtils (Breese and Liu, 2013) to count the number of overlapping reads for each sample.
Genome_build: mm10
Supplementary_files_format_and_content: Tab-delimited text files include read count, CPM and RPKM values for each sample.

extract_protocol:
DNA from infected and non-infected DCs was extracted using the PureGene DNA extraction kit (Gentra Systems).
ATAC-seq libraries were generated from 100,000 BMDMs, as previously described (Buenrostro et al., 2013) and sequenced on an Illumina HiSeq 2500 using 100-bp paired-end reads. We performed ATAC-seq on non-infected BMDMs from four PBS-control mice and three BCG-iv vaccinated mice.

treatment_protocol: BMDMs (1x106 cells) were seeded in 6-well plates supplemented with RPMI without penicillin/streptomycin (1 ml), and incubated overnight at 37°C with 5% CO2. Next day, cells were infected with H37Rv (MOI 1), unless otherwise indicated. Cells were incubated for 4 hours at 37°C with 5% CO2. Subsequently, cells were washed 3x with sterile PBS, and then were supplemented with RPMI without penicillin/streptomycin. CFUs were enumerated at 4 hours, day 3 and day 5 post infection.


title: Th17.3

city: San Diego

first: Klaus

last: Ley

state: CA

address: 9420 Athena Circle

department: Inflammation Biology

data_processing:
Illumina Casava1.7 software used for basecalling.
Reads passing Illumina filters were further filtered for those mapping to tRNA, rRNA, and adapters using Bowtie v1.0.0 (-S --fr)
Filtered reads were mapped against the human mm10 reference using Tophat v1.4.1 (--library-type fr-firststrand)
Reads mapping to the reference genome are further filtered to remove low-complexity reads with PRINSEQ v0.20.3 (-lc_method "dust" -lc_threshold 4)
Sequencing read coverage per gene was counted using HTSeq-count v0.7.1 (-m union -s yes -t exon -i gene_id) and features with 0 counts across all samples were removed
Genome_build: mm10 for mouse samples
Genome_build: hg19 for human samples
Supplementary_files_format_and_content: count matrix with rows as genes and columns as samples

source_name: Spleen

growth_protocol:
Mouse: Naïve CD4 T cells were stimulated with anti-mouse CD3ε and anti-mouse CD28 for 72 h in the presence of recombinant mouse IL-12, recombinant mouse IL-2 and anti-mouse IL-4 for Th1 differentiation, recombinant mouse IL-4, recombinant mouse IL-2, anti-mouse IL-12 and anti-mouse IFNγ for Th2 differentiation, recombinant mouse IL-6, recombinant human TGFβ, anti-mouse IL-4 and anti-mouse IFNγ for Th17 differentiation, and recombinant human TGFβ and recombinant mouse IL-2 for Treg differentiation.
Human: Naïve or Th1 cells were directly isolated from fresh blood peripheral blood mononuclear cell without in vitro culture

extract_protocol:
Mouse CD4 T-cells: Naïve CD4 T cells were isolated from spleens of 8-12 week old mice using EasySep™ Mouse CD4+ T Cell Isolation Kit (STEMCELL Technologies, Vancouver, Canada). Following isolation, purified CD4 T cells were differentiated in vitro in 24-well plate coated with 8 μg/ml anti-mouse CD3ε  (145-2C11) and 8 μg/ml anti-mouse CD28 (37.51) for 72 h in the presence of 25 ng/ml recombinant mouse IL-12, 20 ng/ml recombinant mouse IL-2 and 1 μg/ml anti-mouse IL-4 (11B11) for Th1 differentiation, 50 ng/ml recombinant mouse IL-4, 20 ng/ml recombinant mouse IL-2, 1 μg/ml anti-mouse IL-12 and  1 μg/ml anti-mouse IFN-γ (XMG1.2) for Th2 differentiation, 50 ng/ml recombinant mouse IL-6, 5 ng/ml recombinant human TGF-β1, 1 μg/ml antimouse  IL-4 and 1 μg/ml anti-mouse IFN-γ for Th17 differentiation, and 5 ng/ml recombinant human TGF-β1 and 20 ng/ml recombinant mouse IL-2 for Treg differentiation.
Neutrophils and Promyelocytes: Bone marrow cells were stained with anti-mouse surface markers PerCP-CD45 (30-F11), FITC-Ly6G (1A8), APC-CD115 (AFS98), PE-F4/80 (BM8), PE/Cy7-CD11c (N418), PE/Cy7- TER119 (TER119), PE/Cy7-CD19 (6D5), eFluor®450-CD11c (M1/70), PE-CD4 (GK1.5), PE Siglec-F (E50-2440). Neutrophils were CD45+CD11c+Ly6Ghi and promyelocytes were CD45+CD11c+Ly6Glo. Promyelocytes were further distinguished from monocytes as SSChiCD115-. Cell sorting was done by BD FACSAriaTM II (BD Biosciences, San Diego, CA).
Human Cd4 T cells: Human peripheral blood mononuclear cells were obtained from healthy donors through the LJI Clinical Core in accordance with the La Jolla Institute for Allergy and Immunology Normal Blood Donor Program (VD-057). PBMCs were isolated using SepMate tubes (STEMCELL Technologies). Then, PBMCs were stained by the anti-human surface markers Alexa Fluor® 700 CD3 (UCHT1), eFluor®450 CD4 (OKT4), PE/Cy7-CD25 (BC96), PerCPCy5.5-CD45RA (HI100), Brilliant VioletTM 605-CD45RO (UCHL1), PE-CXCR3 (CEW33D), APC-CCR4 (D8SEE), FITC-CCR6 (R6H1). The CD4 T cell  subsets were sorted by BD FACSAriaTM Fusion (BD Biosciences) based on chemokine receptors expression. Live  CD3+CD4+CD25- cells were further gated using CD45RA and CD45RO  surface markers to differentiate between naïve and memory T cell subsets, respectively. Within the memory T cell subset, Th1 cells were CXCR3+CCR4-CCR6-.
RNA libraries were prepared for sequencing using standard Illumina protocols

tag: cell type

value: Spleen

tag: age

value: 8 weeks

tag: gender

value: female


title: colon adenoma 2 from ApcMin/+ mouse

description: mRNA-seq

source_name: colon adenoma

value: ApcMin/+


title: colon adenoma 3 from ApcMin/+ mouse

description: mRNA-seq


title: colon adenoma 1 from AOM/DSS-treated mouse

description: mRNA-seq


title: colon adenoma 2 from AOM/DSS-treated mouse

description: mRNA-seq


title: AML patient sample12 rep2

city: Seattle

first: Pamela

middle: S.

last: Becker

state: WA

address: 1959 NE Pacific St

department: Medicine

institute: University of Washington

description: Gene expression data from human blood tissue with AML

data_processing:
We used Cufflinks 2.2.1 to get the abundance estimates.
Genome_build: UCSC hg19
Supplementary_files_format_and_content: Tab-delimited text files include FPKM values for each sample

organism: Homo sapiens

source_name: AML patient

growth_protocol: Cells were cultured in long term bone marrow media, which has 15% fetal bovine serum, 15% horse serum, 1X Pen-Strep, 5x10^-5M of b-Mercaptoethanol and 5x10^-5M of Monothioglycerol, plus stem cell growth factor in IMDM.

extract_protocol:
Illumina TruSeq stranded mRNA kit was used according to manufacturer’s instructions.
RNA libraries were prepared for sequencing using standard Illumina protocols.

treatment_protocol: Cells were cultured on RetroNectin coated plates.

tag: disease state

value: AML

value: Blood

tag: cytorisk

value: adverse

tag: prior cr

value: no


title: AML patient sample12 rep1


title: colon adenoma 1 from ApcMin/+ mouse

description: mRNA-seq


title: Th1.3

institute: La Jolla Institute for Allergy and Immunology


title: AML patient sample11 rep2

value: unknown

value: not done


title: non-adenoma colon tissue from ApcMin/+ mouse

description: mRNA-seq


title: AML patient sample9 rep2

value: yes


title: AML patient sample10 rep1


title: AML patient sample9 rep1


title: AML patient sample11 rep1


title: AML patient sample8 rep2


title: AML patient sample10 rep2


title: AML patient sample7 rep2


title: AML patient sample8 rep1


title: AML patient sample7 rep1


title: colon adenoma 3 from AOM/DSS-treated mouse

description: mRNA-seq


title: AML patient sample6 rep2


title: AML patient sample6 rep1


title: AML patient sample5 rep2


title: HF+Ex miRNA rep1

city: Shanghai

first: Jing-Dong

middle: Jackie

last: Han

address: 320 Yue Yang Road

country: China

institute: CAS-MPG Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences

description:
small RNA
CG-1121-P16-miRNA

data_processing:
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for 3' and 5' adapter sequence, and only trimmed sequences 18 to 31 bp were exactly mapped to the mm10 whole genome with SOAP2 v2.20.
Mature miRNAs were annotated with mirBase version 19, quantified similar to Hu et al. and normalized to reads per million isomiR reads (RPM). Lowly expressed miRNAs were filtered out by RPM >= 1 in at least 3 samples. The retained miRNAs were log-transformed for downstream analysis.
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include RPM for each miRNA samples

source_name: liver

extract_protocol:
Livers were removed and flash frozen on dry ice. RNA was harvested using Trizol reagent. 10ug of total RNA was enriched for small RNAs and used to prepare libraries by Berry Genomics, Co. Ltd (Beijing, China).
RNA libraries were prepared for sequencing using standard Illumina protocols

value: liver

value: wild type


title: HF+CR miRNA rep3

description:
small RNA
CG-1121-O15-miRNA


title: HF miRNA rep2

description:
small RNA
CG-1121-K11-miRNA


title: HF+Ex miRNA rep3

description:
small RNA
CG-1121-R18-miRNA


title: HF miRNA rep1

description:
small RNA
CG-1121-J10-miRNA


title: HF+CR miRNA rep1

description:
small RNA
CG-1121-M13-miRNA


title: HF miRNA rep3

description:
small RNA
CG-1121-L12-miRNA


title: AML patient sample4 rep2


title: HF+Ex miRNA rep2

description:
small RNA
CG-1121-Q17-miRNA


title: AML patient sample4 rep1


title: LF+Ex miRNA rep2

description:
small RNA
CG-1121-H8-miRNA


title: AML patient sample3 rep2


title: LF+Ex miRNA rep1

description:
small RNA
CG-1121-G7-miRNA


title: AML patient sample3 rep1


title: AML patient sample2 rep1


title: HF+CR miRNA rep2

description:
small RNA
CG-1121-N14-miRNA


title: LF+CR miRNA rep1

description:
small RNA
CG-1121-D4-miRNA


title: LF miRNA rep3

description:
small RNA
CG-1121-C3-miRNA


title: AML patient sample1 rep1


title: LF+CR miRNA rep3

description:
small RNA
CG-1121-F6-miRNA


title: AML patient sample2 rep2


title: AML patient sample1 rep2


title: LF+CR miRNA rep2

description:
small RNA
CG-1121-E5-miRNA


title: AML patient sample5 rep1


title: LF miRNA rep2

description:
small RNA
CG-1121-B2-miRNA


title: HCT-116 APC ChIP-seq

description: ChIP-seq

data_processing:
Fastq files were analyzed for per base sequence quality.  97-98% of reads were confirmed to have passed quality control filters.
Burrows-Wheeler Aligner was used to perform alignment to the UCSC hg19 reference genome, generating bam files.
Bam alignment files were uploaded to Galaxy and peak calling was performed using the Model-based Analysis of ChIP-Seq version 2 (MACS2) tool, generating peak scores, p-values and false discovery rates (FDR).  Parameter tuning was performed to identify the p-value threshold (p < 0.001) at which input/background signal was lowest while positive control peaks in the AXIN2, DKK1 and MYC loci were still detected.  Peak regions were annotated using the RefGene database and distances to transcription start sites were determined and used to assign peaks to genes.
Genome_build: hg19
Supplementary_files_format_and_content: The file Annotated_peaks_APC_ChIP-seq_3.xlsx is a spreadsheet listing the genomic peaks identified in the anti-APC ChIP-seq analysis, along with the peak score, the identity of the nearest gene and the location of the peak relative to the transcription start site of that gene.

source_name: HCT-116 colon cancer cell line

growth_protocol: HCT-116 cells were purchased from the American Type Culture Collection (ATCC) and cultured in McCoy's 5A medium (GIBCO catalog #16600082) supplemented with 10% fetal bovine serum.  Cells had been cultured for fewer than 20 passages at the time of the experiment.

extract_protocol:
6 x 106 HCT-116 cells were seeded in each 150-mm dish.  Cells were crosslinked after 24 hrs. with 1% formaldehyde for 10 minutes before quenching by 0.125 M glycine.  Cells were washed and scraped into Dulbecco’s PBS, pelleted and resuspended in cold 10 mM HEPES pH 7.5, 10 mM EDTA, 0.5 mM EGTA, 0.75% Triton X-100, 1 mM PMSF with mammalian protease inhibitor cocktail (Sigma-Aldrich) and incubated 10 minutes at 4oC with rotation.  Cells were again pelleted and resuspended in cold 10 mM HEPES pH 7.5, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 1 mM PMSF and protease inhibitors and rotated 10 minutes at 4oC.  Nuclei were pelleted and resuspended in 1 mL of cold 25 mM Tris-HCl pH 7.5, 150 mM NaCl, 5 mM EDTA, 0.1% SDS, 1% Triton X-100, 0.5% Sodium Deoxycholate, 1 mM PMSF with protease inhibitors.  Probe-based sonication at 4oC was performed using 30 pulses of 10-seconds each at 35% amplitude.  Fragment size was reduced by dividing the resulting supernatant into 300 μL aliquots and mixing each with 100 μL of 2 M sucrose, 320 mM KCl, 80 mM HEPES pH 7.5, 14 mM ATP, 24 mM CaCl2 and 4 µL of 50 U/µL micrococcal nuclease (Affymetrix, Inc.).  Digest was incubated 15 minutes at room temperature and quenched by mixing on ice with 45 µL of 200 mM EDTA, 20 mM EGTA.  An equal volume of 16.7 mM Tris-HCl pH 8, 167 mM NaCl, 1.2 mM EDTA, 0.01% SDS, 1.1% Triton X-100, 1 mM PMSF, with protease inhibitors was added to each sample to dilute it.  Pre-clearing of input was performed using 2-hour incubation with 5 μg rabbit IgG antibody and 40 μL of pre-equilibrated Protein G Dynabead slurry (Thermo Fisher Scientific) at 4oC with rotation.  100 μL of the resulting supernatant was saved as “pre-cleared input”.  The remainder was used for anti-APC ChIP in combination with 10 μg of anti-APC antibody (a polyclonal antibody recognizing the C-terminal 50 amino acids of APC, catalog #A300-981A, Bethyl Laboratories, Inc.).  Immunoprecipitations were rotated overnight at 4oC and mixed with 40 μL of pre-equilibrated Protein G Dynabead slurry and rotated 3 hours at 4oC.  Beads were collected by magnet and washed twice with cold 50 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 mM EDTA, 0.1% SDS, 1% NP-40, 0.5% sodium deoxycholate, 1 mM PMSF, and protease inhibitors, twice with cold 50 mM Tris-HCl pH 8.0, 500 mM NaCl, 1 mM EDTA, 0.1% SDS, 1% NP-40, 0.5% sodium deoxycholate, 1 mM PMSF, and protease inhibitors, twice with cold 50 mM Tris-HCl pH 8.0, 250 mM LiCl, 1 mM EDTA, 1% NP-40, 0.5% sodium deoxycholate, 1 mM PMSF, and protease inhibitors, and twice with cold 10 mM Tris-HCl pH 8.0, 1 mM EDTA, 1 mM PMSF, and protease inhibitors.  All washes were accompanied by 5 minute rotation at 4oC.  Elution was performed by the addition of 210 μL room temperature Elution Buffer (50 mM Tris-HCl B1pH 8, 10 mM EDTA, 1% SDS) to the washed beads, and 45 minute incubation at 65oC with occasional gentle resuspension of beads.  Supernatant was isolated and crosslinks were reversed by mixing with 16 μL of 5M NaCl and incubation at 65oC for 12 hrs.  210 μL TE was added to ChIP reactions, while 300 μL TE was added tothe pre-cleared input samples.  8 μL of 10 mg/mL RNase A was mixed with each sample and incubated 2 hrs at 37oC.  4 μL of 25 mg/mL Proteinase K was mixed with each sample and followed by 2 hours incubation at 55oC.  Samples were extracted twice with phenol:chloroform:isoamyl alcohol (25:24:1), once with chloroform, anB1d mixed with 1/10th volume of sodium acetate pH 5.2 and 60 μg glycogen for eB1thanol precipitation.  Yields from multiple parallel α-APC ChIP reactions were pooled to obtain the 10 ng required for ChIP-seq library preparation.  The PCR purification kit (QIAGEN) was used to clean up reactions prior to library preparation.
Library preparation for ChIP-seq was performed with the NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina, using adaptors AD005 and AD019 (New England Biolabs) for the input and ChIP libraries, respectively.  Size selection was performed by E-gel (Life Technologies, Inc.) to obtain fragments ranging in size from 300-400 bp.  Next-generation sequencing (50 bp, single-end) was performed by the OSU Genomics Shared Resource using a HiSeq 2500 instrument (Illumina, Inc.).

tag: cell line

value: colon carcinoma

value: APC wild-type, CTNNB1{delta}Ser45

tag: chip antibody

value: anti-APC (a polyclonal antibody recognizing the C-terminal 50 amino acids of APC, catalog #A300-981A, Bethyl Laboratories, Inc.)


title: HCT-116 Input ChIP-seq

description: ChIP-seq

treatment_protocol: 6 x 106 HCT-116 cells were seeded in each 150-mm dish.


title: LF+Ex miRNA rep3

description:
small RNA
CG-1121-I9-miRNA


title: UT3_RNA

city: Detroit

first: Aliccia

last: Bollig-Fischer

address: 4100 John R

department: Oncology

institute: Karmanos Cancer Institute & Wayne State University

data_processing:
PVUseq and RNAseq: Basecalls were performed using Illumina's CASAVA vesrsion 1.8.2
PVUseq and RNAseq: quality of the sequenced reads were assessd using FastQC from Babraham Bioinformatics
PVUseq and RNAseq: reads were aligned to Human Genome Consortium's reference human genome (hg19) using Novoalign V2.08.03
PVUseq: PVU-cognate sequences were determined using the PERL script obtained from Sun et al, Cell Rep 2013, 3:567-576. differential 5hmC levels were calculated in R using DESeq from Bioconductor; the UCSC transcriptome (hg19) was used as annotation reference
RNAseq: differential expression levels were calculated in R using DESeq from Bioconductor; the UCSC transcriptome (hg19) was used as annotation reference
Genome_build: hg19
Supplementary_files_format_and_content: tab-delimited, significant fold changes for PVU and RNA data from DESeq pipeline and correlation statistic computed using a min-max normalization and the distance to the first diagonal (x=y)

source_name: Vehicle treated Triple negative breast cancer cell line

growth_protocol: Triple negative breast cancer cell line MDA-MB-468 was cultured in DMEM medium (HyClone, Logan, UT) supplemented with 10% FBS (HyClone, Logan, UT).

extract_protocol:
DNA extraction for PVU-Seq: DNeasy Blood and tissue kit (Qiagen, Valencia, CA) was used to extract genomic DNA from the fresh frozen tissues and MDA-MB-468 cells. RNaseA treatment was done according to the manufacturer’s instructions [4µL of RNaseA (100mg/mL)] to obtain RNA free genomic DNA. Extraction of the RNA was done using the RNeasy plus kit (Qiagen, Valencia, CA) according to manufacturer's instructions
ThruPLEX-FD prep kit (Rubicon Genomics, Ann Arbor, MI) was used to prepare DNA libraries for PVU-Seq. Illumina TruSeq (Low Sample) RNA library preparation was used according to the manufacturer's instructions

treatment_protocol: MDA-MB-468 cells were treated with 10 µM CAT-SKL for 24 hours in triplicate for DNA extraction for PVU-Seq or RNA extraction for RNA seq. The authenticity of the cell line was verified by STR analysis.

value: triple negative breast cancer cell line MDA-MB-468

tag: dna digestion

value: not applicable

value: vehicle


title: UT1_RNA

value: Vehicle treated Triple negative breast cancer cell line


title: UT2_RNA


title: Control rep2

data_processing:
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome using tophat v1.4.1 with default parameters except -N 3
Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using cufflinks v1.3.0 and low expressed reads were filtered out by RPKM >= 1 in at least 2 samples. The retained genes were log-transformed for downstream analysis.
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include RPKM values for each mRNA samples.

molecule: polyA RNA

source_name: Hepatoma Hepa1-6 cells

growth_protocol: Hepa1-6 cells were cultured in DMEM plus 10% FBS media.

extract_protocol:
RNA was harvested using Trizol reagent. 10ug of total RNA was enriched for polyA-RNA and used to prepare libraries by Berry Genomics, Co. Ltd (Beijing, China).
RNA libraries were prepared for sequencing using standard Illumina protocols

treatment_protocol: After lentivirus transduction cells were transferred to a new plate with fresh growth media containing 4 μg/ml puromycin and selected for 3 weeks prior to RNA extraction.


title: Chd1KD rep1

value: Chd1 knock down


title: UT3_Pvu

data_processing:
Library strategy: PVU-Seq
PVUseq and RNAseq: Basecalls were performed using Illumina's CASAVA vesrsion 1.8.2
PVUseq and RNAseq: quality of the sequenced reads were assessd using FastQC from Babraham Bioinformatics
PVUseq and RNAseq: reads were aligned to Human Genome Consortium's reference human genome (hg19) using Novoalign V2.08.03
PVUseq: PVU-cognate sequences were determined using the PERL script obtained from Sun et al, Cell Rep 2013, 3:567-576. differential 5hmC levels were calculated in R using DESeq from Bioconductor; the UCSC transcriptome (hg19) was used as annotation reference
RNAseq: differential expression levels were calculated in R using DESeq from Bioconductor; the UCSC transcriptome (hg19) was used as annotation reference
Genome_build: hg19
Supplementary_files_format_and_content: tab-delimited, significant fold changes for PVU and RNA data from DESeq pipeline and correlation statistic computed using a min-max normalization and the distance to the first diagonal (x=y)

value: PvuRts1l digested


title: Chd1KD rep2


title: CAT1_RNA

source_name: CAT-SKL treated Triple negative breast cancer cell line

value: anti-oxidant CAT-SKL


title: UT2_Pvu


title: CAT2_RNA

value: CAT-SKL treated Triple negative breast cancer cell line


title: UT1_Pvu


title: CAT3_RNA


title: CAT1_Pvu


title: T_TN_2

source_name: Triple negative tumor 2

value: Triple negative tumor 2

value: Triple negative tumor


title: CAT2_Pvu


title: HF+Ex mRNA rep3

data_processing:
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome using tophat v1.4.1 with default parameters except -N 3
Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using cufflinks v1.3.0 and low expressed reads were filtered out by RPKM >= 1 in at least 3 samples. The retained genes were log-transformed for downstream analysis.
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include RPKM values for each mRNA samples.

extract_protocol:
Livers were removed and flash frozen on dry ice. RNA was harvested using Trizol reagent. 10ug of total RNA was enriched for polyA-RNA and used to prepare libraries by Berry Genomics, Co. Ltd (Beijing, China).
RNA libraries were prepared for sequencing using standard Illumina protocols


title: HF+Ex mRNA rep2


title: N_TN_2

source_name: normal tissue matched to triple negative tumor 2

value: normal


title: N_TN_1

source_name: normal tissue matched to triple negative tumor 1


title: T_TN_1

source_name: Triple negative tumor 1

value: Triple negative tumor 1


title: LF miRNA rep1

description:
small RNA
CG-1121-A1-miRNA

value: liver


title: HF+Ex mRNA rep1

value: wild type


title: Control rep1


title: Mem_AKJ_D3630

city: Atlanta

first: Rama

middle: S

last: Akondy

state: GA

address: 1462 CLifton Rd

department: Emory Vaccine Center

institute: Emory University

data_processing:
RNA was aligned to the hg19 genome using Star aligner version 2.4.
Differentially expressed genes were then identified using the Bioconductor package, DESeq2
Significant genes were defined as those with an adjusted p-value of less than 0.01 and an average expression of at least 20 reads.
Supplementary_files_format_and_content: RNAseq.csv is an excel file that contains  feature list for each of the 14 samples. The numbers in the columns are normalized raw counts.

source_name: PBMC

extract_protocol:
Total RNA was extracted from sorted cells using the Qiagen Allprep DNA/RNA microkit.
Samples were sent to Hudson Alpha for RNAseq analysis. The low input protocol that uses the Ovation RNA-Seq System V2 kit (Nugen) for initial RNA amplification was used.  25 million PE 125x reads were performed on each sample.

treatment_protocol: Donors vaccinated with YFV-17D; fresh PBMC isolated from whole blood using CPT tubes (BD) and used for sorting populations of interest

tag: donor

value: AKJ

tag: day

value: Day 3630 post YFV-17D

value: sorted A2-NS4B214 tetramer+ CD8 T cells


title: HF+CR mRNA rep2


title: HF mRNA rep3


title: HF+CR mRNA rep1


title: Mem_E07_D3409

value: Day 3409 post YFV-17D


title: Mem_EVC422_D4745

value: Day 4745 post YFV-17D


title: D14_YF661

value: Day 14 post YFV-17D


title: HF mRNA rep1


title: LF+Ex mRNA rep1


title: Mem_MCB.D1346

value: MCB

value: Day 1346 post YFV-17D


title: LF+Ex mRNA rep2


title: HF mRNA rep2


title: D14_YF679


title: LF+CR mRNA rep2


title: LF+CR mRNA rep1


title: Naive_YF_MCB

value: YFMCB

value: sorted CD45RA+ CCR7+ CD8 T cells


title: LF+CR mRNA rep3


title: HF+CR mRNA rep3


title: Naive_YF_RTA336

value: Day 1349 post YFV-17D


title: D14_YF631


title: LF mRNA rep3


title: CAT3_Pvu

value: CAT-SKL treated Triple negative breast cancer cell line

value: triple negative breast cancer cell line MDA-MB-468

value: PvuRts1l digested

tag: treatment

value: anti-oxidant CAT-SKL


title: Naive_YF653

value: Day 28 post YFV-17D


title: Naive_YF661


title: LF mRNA rep2

value: liver

value: wild type


title: LF mRNA rep1


title: Memory_YF_RTA336


title: Naive_AKJ

value: AKJ

value: Day 3630 post YFV-17D


title: MYC_sas2D

city: Daejeon

first: Daeyoup

last: Lee

address: 291 Daehak-ro, Yuseong-gu

department: Biological Sciences

country: South Korea

institute: Korea Advanced Institute of Science and Technology

data_processing:
The quality of fastq files were checked by fastqc and trimmed by trim_galore
The qualified fastq files were aligned to SacCer3 genome fa file using Bowtie2 v2.2.3. Then, the aligned files were compressed and sorted by samtools v1.3.
The sorted BAM files of biological duplicates were compared by MACS2, bam2wig and deeptools.
Genome_build: sacCer3
Supplementary_files_format_and_content: bigwig files were generated by MACS2, bam2wig and deeptools.

organism: Saccharomyces cerevisiae

source_name: S.cerevisiae cells

growth_protocol: Cell culture were inoculated twice. Cell cultures were inoculated with shaking for aeration at proper temperatures.

extract_protocol:
Every ChIP-seq cells were grown up to mid-log phase and harvested after final 1% formaldehyde fixation and 125mM glycine quencing. Cells were harvested by centrifugation at 4 Celcius degrees and the cell pellets were washed by 15mL of pH7.5 TBS buffer per a conical tube. ChIP cells were disrupted by glass bead vortexing in ChIP  lysis buffer and sonicated by Sonic Dismembrator Model 500, Fisher Scientific. Debris of the sonicate was excluded by centrifugation by 14,800rpm at 4 Celcius degree. IP samples were aliqouted from the sonicate and immunoprecipiated by proper amount of antibody and protein A/G beads, washed by ChIP  lysis buffer for over-night at 4 Celcius degrees. IP samples were washed and eluted by proper buffers in  Sigmaprep spin column. Proteins in both IP and input samples were degraded by 2hr 30ug proteinase K rxn and de-crosslinked at 65 Celcius degrees for at least 8hrs. The IP and input DNA were eluted by Quiagen PCR purification kit.
Total RNA for mRNA-seq was prepared by the hot-phenol extraction.
For preparation of MNase-seq library, formaldehyde-fixed cell pellets were resuspended in 10 ml of pre-incubation buffer (20 mM EDTA, 0.7 M mercaptoethanol), washed, and resuspended in 2 ml of zymolyase buffer (1 M sorbitol, 50 mM Tris-Cl, pH 8.0, 5 mM mercaptoethanol) containing 2.5 mg ml-1 zymolyase (20T; US Biological). Lysed cells were spheroplasted at 30°C  for 20 min and chromatin was isolated. Pelleted nuclei were digested with 200 units of MNase (NEB) at 37°C for 20 min (confirmed as a typical digestion condition by comparison of fragment sizes, data not shown), and then reverse cross-linked in 10 mM EDTA and 1% SDS buffer with proteinase K for 6 hours at 37°C. The DNA was extracted with phenol/chloroform, purified by ethanol precipitation, and resolved by 2% agarose gel electrophoresis in TAE buffer.
Yeast cells (2.5 million) were harvested at O.D. 0.6~0.8/mL (mid-log phase), and spheroplasted in 200 μl of Sorbitol buffer (1.4 M Sorbitol, 40 mM Tris-HCl, pH 7.5, 0.5 mM MgCl2) with 10 μl of 50 mg ml-1 of zymolyase (20T; US Biological). After washing twice with 100 μl of ice-cold Sorbitol buffer, the spheroplasts were incubated in fresh TD buffer (20 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 20% dimethylformaldehyde) with transposase at 37°C for 4 hours. QIAquick PCR purification kit was used to purify DNA fragments, and transposition reactions and PCR amplifications were performed.
General Manual of Nextflex ChIP library preparation kit. mRNA isolation and mRNA-seq library preparation were performed using the NEXTflex Rapid Directional mRNA-Seq Bundle.

treatment_protocol: For ChIP and MNase: Final 1% formaldehyde fixation and 125mM glycine quencing were performed before cell harvest.

tag: antibody

value: homemade; prepared by Lee Lab

tag: phase

value: mid-log phase

tag: medium

value: YPR; Galactose induction


title: LF+Ex mRNA rep3

tag: strain

tag: tissue


title: Naive_EVC422

value: Day 4745 post YFV-17D

tag: cell type


title: H3_sas2D

tag: genotype

value: YPD


title: H3_sas2Dset2D


title: H4ac_H3_sas2D

value: 06-866; Millipore


title: Adult GF Male Replicate 3

status: Public on Dec 12 2017

city: Singapore

first: jinmiao

last: chen

address: 8A Biomedical Grove, #04-06 Immunos

country: Singapore

institute: Singapore Immunology Network

data_processing:
reads were mapped to mouse reference genome GRCm38 using STAR. Reads per gene were counted using featureCounts. Mouse reference genome .fasta file and gene annotation .gtf file were downloaded from Gencode version M9
Genome_build: GRCm38

source_name: Brain microglia

extract_protocol:
Total RNA was extracted using the Ambion mirVana™ miRNA Isolation Kit (Ambion® Thermo Fisher Scientific, Waltham, MA, USA) according to manufacturer's protocol.
cDNA libraries were prepared from 2 ng total RNA starting material and 1 ul of a 1:50,000 dilution of ERCC RNA Spike in Controls (Ambion® Thermo Fisher Scientific) using the SMARTSeq v2 protocol (Picelli et al., 2014) with the following modifications: 1. Addition of 20 µM TSO; 2. Use of 250 pg cDNA with 1/5 reaction of Illumina Nextera XT kit (Illumina, San Diego, CA, USA). OR cDNA libraries were prepared using 50 ng of total RNA and 2ul of a 1:2000 dilution of ERCC RNA Spike in Controls (Ambion). The fragmented mRNA samples were subjected to cDNA synthesis using Illumina TruSeq® RNA sample preparation kit version 2 (Low-Throughput protocol) (Illumina, San Diego, CA, USA) according to manufacturer's protocol, except for the following modifications: 1. use of 13 PCR cycles, 2. two additional round of Agencourt Ampure XP SPRI beads (Beckman Courter) to remove >600bp double stranded cDNA.

value: Brain microglia

tag: developmental stage

value: Adult (2 months)

tag: microbiota status

value: Germ-free (GF)

tag: extraction method

value: TRUseq


title: H4ac_H3_sas2Dset2D


title: Adult GF Male Replicate 2


title: Adult SPF Male Replicate 2

value: Specfic-pathogen-free (SPF)


title: tomato not inoculated rep 2

city: Castanet tolosan

first: Sylvain

last: Raffaele

address: 28 chemin de borde rouge

country: France

institute: INRAE

data_processing:
HiSeq control software 2.2.38, RTA 1.18.61.0, Illumina Casava1.8.2 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to whole genomes using the RNA-seq analysis function of the CLC genomics software with default settings.
Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using the RNA-seq analysis function of the CLC genomics software with default settings.
Genome_build: TAIR10 (A. thaliana) - version 3 (S. lycopersicum)
Supplementary_files_format_and_content: tab-delimited text files include RPKM values for each Sample

organism: Solanum lycopersicum

source_name: 4-week old plants mature leaves

growth_protocol: Plants were grown in Jiffy pots under controlled conditions at 22°C, with a 9 hour light period and a light intensity of 120 µmol/m2/s 4 weeks prior to infection

extract_protocol:
The edge of developed necrotic lesions (15 to 30 mm diameter) was harvested and stored in liquid nitrogen. Samples were ground with metal beads (2.5 mm) in a Retschmill apparatus (24hertz for 2x1min) before solubilizing in 1mL Trizol reagent (ThermoFisher) and left for 5 min at RT. Chloroform (200 µL) was added and mixed thoroughly before incubating for 3 min at RT. After centrifugation at ~12,000g (4°C) for 15min, the upper aqueous phase was recovered and nucleic acids were precipitated by adding 2µL GlycoBlue (Ambion) and 500µL isopropanol (10 min at -20°C). After centrifugation at ~15,000g (4°C) for 15min, pellets were washed twice with 70% ethanol before drying and resuspended in RNAse-free water. To eliminate chloroform traces, water resuspended nucleic acids were further cleaned using an RNA extraction kit (Quiagen) following manufacturer’s instructions. Genomic DNA was removed by DNase treatment (TURBO DNase; Ambion) following manufacturer’s instructions. The quality and concentrations of RNAs preparations were assessed with an Agilent apparatus and chips (nano).
RNA libraries were prepared for sequencing using standard Illumina protocols

treatment_protocol: Mature leaves of 4 week-old Arabidopsis thaliana and Solanum lycopersicum (Heinz genotype) were inoculated or not with the S. sclerotiorum strain 1980. An agar plug (5mm in diameter) containing actively growing S. sclerotiorum mycelium was placed on the adaxial surface of leaves and plants were maintained at 80% humidity

tag: genotype/variation

value: cv Heinz

value: healthy


title: Adult GF Male Replicate 1


title: ROR1+ biological replicate 1

city: Darlinghurst

first: Andrian

last: Yang

state: NSW

address: 405 Liverpool Street

country: Australia

institute: Victor Chang Cardiac Research Institute

data_processing:
RNA-seq reads were processed using the Falco framework (Yang et al, Bioinformatics 2016 btw732)
HISAT2 was used for aligning the reads to the hg19 genome with parameters --tmo (added as default by the Falco framework)
featureCount was then used to perform read quantification using GTF from Gencode with parameters -s 2 -t exon -g gene_name --primary
Genome_build: hg19
Supplementary_files_format_and_content: CSV file containing raw counts values for each gene per sample

source_name: ROR1+ cells derived from human pluripotent stem cells

growth_protocol: Human ES cells were differentiated towards heterogeneous cultures containing lens cells via the protocol of Yang et al, FASEB J. 2010 Sep;24(9):3274-83.

extract_protocol:
RNA was collected immediately after cell separation and purified using an Isolate II RNA purification kit; RNA purity and quality was confirmed using a Bioanalyser.
RNA-seq libraries were prepared using a TruSeq Stranded Total RNA Sample Preparation Kit. Samples were sequenced on an Illumina HiSeq 2500 instrument using 2x100 paired end reads.

treatment_protocol: Presumptive lens cells were purified via magnetic cell separation using an anti-human ROR1 antibody.

value: ROR1+ cells

value: Resemble human lens epithelial cells


title: tomato not inoculated rep 1


title: ROR1+ biological replicate 2


title: mRNA T. muris exosomes rep 1

city: Smithfield

first: Javier

last: Sotillo

address: 14-88 McGregor rd

department: AITHM

institute: James Cook University

description: mRNA

data_processing:
STAR aligner was used to do the alignment
RSEM was used to estimate transcript abundance using the trichuris genome and gene model
Genome_build: Assembly= TMUE2.2; bioproject= PRJEB126
Supplementary_files_format_and_content: bed and .tsv files were generated

organism: Trichuris muris

source_name: Extracellular vesicles

growth_protocol: Adult worms were harvested from the caecum of infected mice 5 weeks after infection, washed in PBS containing 5× antibiotic/antimycotic (AA) and cultured in 6-well plates for 5 days in RPMI containing 1× AA, at 37°C and 5% CO2.

extract_protocol:
EVs obtained from one batch of worms were used. ODG fractions with a density between 1.06 and 1.08 (fractions containing pure EV samples after TRPS analysis) were pooled and excess Optiprep® solution was removed by buffer exchanging. Total RNA was extracted using the mirVana miRNA Isolation Kit (ThermoFischer) according to the manufacturer’s instructions.
mRNA was prepared for sequencing using Illumina TruSeq stranded mRNA-seq library preparation kit according to the manufacturer’s instructions.

treatment_protocol: The media obtained during the first 4 h after parasite culturing was discarded for further analysis. Dead worms were removed and ES products were collected daily, subjected to sequential differential centrifugation at 500 g, 2000 g and 4000 g for 30 min each to remove eggs and parasite debris. For the isolation of ES products, media was concentrated using a 10 kDa spin concentrator and stored at 1.0 mg/ml in PBS at -80°C until required. For the isolation of EVs, the media obtained after differential centrifugation was processed as described previously. Briefly, ES products were concentrated using a 10 kDa spin concentrator, followed by centrifugation for 45 min at 12,000 g to remove larger vesicles. A MLS-50 rotor (Beckman Coulter, USA) was used to ultracentrifuge the supernatant for 3 h at 120,000 g and the resultant pellet was resuspended in 70 μl of PBS and subjected to Optiprep® discontinous gradient (ODG) separation. One mL of 40%, 20%, 10% and 5% iodixanol solutions prepared in 0.25 M sucrose, 10 mM Tris-HCl, pH 7.2, was layered in decreasing density in an ultracentrifuge tube, and the 70 µl containing the resuspended EVs was added to the top layer and ultracentrifuged at 120,000 g for 18 h at 4°C. Seventy (70) µl of PBS was added to the control tube prepared as described above. A total of 12 fractions were recovered from the ODG, and the excess Optiprep® solution was removed by buffer exchanging with 8 ml of PBS containing 1× EDTA-free protease inhibitor cocktail (Santa Cruz, USA) using a 10 kDa spin concentrator.

value: Adult worms

tag: vesicle type

value: Exosome-like vesicles


title: E18.5 GF Male Replicate 3

value: SMARTseq


title: Adult SPF Female Replicate 1


title: Adult GF Female Replicate 2


title: Adult GF Female Replicate 1


title: Adult GF Female Replicate 3


title: E18.5 SPF Male Replicate 2


title: E18.5 GF Female Replicate 2


title: Adult SPF Female Replicate 2


title: E18.5 GF Male Replicate 2


title: E18.5 GF Female Replicate 3


title: E18.5 SPF Male Replicate 1


title: FLAG_sas2D

value: F1804; SIGMA;

value: mid-log phase


title: E18.5 SPF Female Replicate 3


title: E18.5 SPF Female Replicate 2


title: Adult SPF Male Replicate 1


title: E14.5 GF Replicate 2


title: E14.5 GF Replicate 3


title: E14.5 GF Replicate 1


title: E18.5 SPF Female Replicate 1


title: tomato not inoculated rep 3


title: E14.5 SPF Replicate 3


title: E14.5 SPF Replicate 1


title: Lung_9M_oxBS_96

city: Toronto

first: Art

last: Petronis

state: Ontario

address: 250 College St

department: Campbell Family Mental Health Research Institute

country: Canada

institute: Centre for Addiction and Mental Health

description:
technical_replicate3
sequencing_replicate1

data_processing:
The libraries were sequenced on the HiSeq 2500 platform (Illumina) using custom sequencing primers.
The FASTQ reads were quality trimmed using Trimmomatic (24695404) for trailing base with phred score < 30 and all reads with post-trimming length < 50 bp.
The trimmed reads were then aligned using Bismark version 0.14 (21493656) and Bowtie 2 version 2.2.2 (22388286).
The aligned reads were further filtered to only include reads that have start and end positions that match the padlock probe annealing arm sequence with no more than 1 mismatch.
The filtered reads were subsequently PCR de-duplicated using unique molecular identifiers.
The filtered read pairs were then converted to methylation calls using the Bismark methylation extractor tool (21493656).
Genome_build: mm10
Supplementary_files_format_and_content: Each txt file contain the following information in order: chrosome #, genomic coordinate, strand, unmethylation count, methylation count, CHH/CHG/CG context of cytosine, exact sequence of the previous context

source_name: Lung

growth_protocol: The mice had ad libitum access to food and water

extract_protocol:
DNA isolation was performed using standard phenol chloroform methods.
Bisulfite padlock probes were designed using the ppDesigner 2.0 software (22306810) for mouse chromosome 7 (mm10).  The probe and library preparation was performed based on previously published protocol (ibid).

tag: age (in months)

tag: Sex

value: male

tag: treatment (bisulfite/oxybisulfite)

value: oxybisulfite


title: E18.5 SPF Male Replicate 3


title: Lung_9M_oxBS_95


title: Lung_9M_oxBS_88


title: Lung_9M_oxBS_92


title: Lung_9M_oxBS_94


title: Lung_9M_oxBS_93


title: Lung_9M_oxBS_89


title: Lung_9M_oxBS_87


title: Lung_9M_oxBS_90


title: Lung_9M_oxBS_86


title: Lung_9M_oxBS_85


title: Lung_9M_oxBS_81


title: Lung_9M_oxBS_82


title: Lung_9M_oxBS_83


title: Lung_9M_oxBS_80


title: Lung_9M_oxBS_78


title: Lung_9M_oxBS_79


title: Lung_9M_oxBS_75


title: Lung_9M_oxBS_74


title: Lung_9M_oxBS_91


title: Lung_9M_oxBS_77


title: Lung_9M_oxBS_76


title: Lung_9M_oxBS_84


title: Lung_9M_oxBS_71


title: E14.5 SPF Replicate 2


title: Lung_9M_oxBS_70


title: Lung_9M_oxBS_73


title: Lung_9M_oxBS_69


title: Lung_9M_oxBS_68


title: Lung_9M_oxBS_62

description:
technical_replicate2
sequencing_replicate1


title: Lung_9M_oxBS_63


title: Lung_9M_oxBS_59


title: Lung_9M_oxBS_64


title: Lung_9M_oxBS_67


title: Lung_9M_oxBS_66


title: Lung_9M_oxBS_56


title: Lung_9M_oxBS_60


title: Lung_9M_oxBS_53


title: Lung_9M_oxBS_72


title: Lung_9M_oxBS_52


title: Lung_9M_oxBS_58


title: Lung_9M_oxBS_55


title: Lung_9M_oxBS_51


title: Lung_9M_oxBS_47


title: Lung_9M_oxBS_44


title: Lung_9M_oxBS_46


title: Lung_9M_oxBS_43


title: Lung_9M_oxBS_65


title: Lung_9M_oxBS_48


title: Lung_9M_oxBS_39


title: Lung_9M_oxBS_57


title: Lung_9M_oxBS_41


title: Lung_9M_oxBS_42


title: Lung_9M_oxBS_61


title: Lung_9M_oxBS_38


title: Lung_9M_oxBS_36


title: Lung_9M_oxBS_37


title: Lung_9M_oxBS_34


title: Lung_9M_oxBS_33

description:
technical_replicate1
sequencing_replicate1


title: Lung_9M_oxBS_35


title: Lung_9M_oxBS_32


title: Lung_9M_oxBS_54


title: Lung_9M_oxBS_50


title: Lung_9M_oxBS_30


title: Lung_9M_oxBS_31


title: Lung_9M_oxBS_27


title: Lung_9M_oxBS_29


title: Lung_9M_oxBS_26


title: Lung_9M_oxBS_49


title: Lung_9M_oxBS_45


title: Lung_9M_oxBS_25


title: Lung_9M_oxBS_23


title: Lung_9M_oxBS_21


title: Lung_9M_oxBS_24


title: Lung_9M_oxBS_22


title: Lung_9M_oxBS_20


title: Lung_9M_oxBS_15


title: Lung_9M_oxBS_18


title: Lung_9M_oxBS_19


title: Lung_9M_oxBS_16


title: Lung_9M_oxBS_14


title: Lung_9M_oxBS_13


title: Lung_9M_oxBS_17


title: Lung_9M_oxBS_40


title: Lung_9M_oxBS_10


title: Lung_9M_oxBS_11


title: Lung_9M_oxBS_8


title: Lung_9M_oxBS_6


title: Lung_9M_oxBS_9


title: Lung_9M_oxBS_5


title: Lung_9M_oxBS_7


title: Lung_9M_oxBS_4


title: Lung_9M_oxBS_1


title: Lung_9M_oxBS_2


title: Lung_9M_oxBS_3


title: Lung_9M_oxBS_12


title: 10X46_1_TTTCAGTGACAGCT-1

city: Stockholm

first: Sten

last: Linnarsson

address: Scheeles väg 1

department: Medical Biochemistry and Biophysics

country: Sweden

institute: Karolinska Institutet

description: Processed data file: 10X_expression_data.tab.gz

data_processing:
Processing was performed with the 10X Chromium cellranger pipeline version 1.1 using default settings
The Unique Molecular Identifier and cellular barcode corresponding to each read have been appended to the end of the read id in the fastq files, separated by an underscore.
Genome_build: UCSC mm10 (GRCm38)
Supplementary_files_format_and_content: 10X_expression_data.tab: Tab-delimited text file of total RNA molecules in each cell.

source_name: dentate gyrus, postnatal day 16

growth_protocol: Male and female wild type CD-1 mice (Charles River) at postnatal days 12, 16, 24, and 35 were used.

extract_protocol:
Mice were sacrificed by an overdose of Isoflurane or Ketamin/Xylazine, followed by perfusion through the left ventricle with artificial cerebrospinal fluid, equilibrated in 95%O2/5%CO2 on ice or 4°C. The dentate gyrus was then microdissected from 300 μ m vibratome brain sections. Single-cell suspensions were prepared using the Papain kit (Worthington) with 25-35min enzymatic digestion followed by manual trituration using a BSA-coated p1000 pipette or fire-polished Pasteur pipettes. Sampling was carried out on two experimental days, each day pooling from male and female mice in a single experiment. The dentate gyrus of male (day 1 p35, day 2 p24) and female (day 1 p12, day 2 p16) mice were dissected and dissociated separately. Suspensions were pooled at equal ratios.
1000 cells/μl were resuspended in artificial cerebrospinal fluid and added to the Reverse transcription mix to aim for sampling of around 3500 cells. All downstream cDNA synthesis (13 PCR cycles), library preparation and sequencing were carried out as instructed by the manufacturer (10X Genomics Chromium Single Cell Kit Version 1).

tag: strain/background

value: wild type

tag: postnatal day

value: dentate gyrus

tag: cell cluster


title: 10X46_1_TTTGACTGTTCATC-1

source_name: dentate gyrus, postnatal day 24

value: Granule-mature


title: 10X46_1_TTTGCATGTGCACA-1


title: 10X46_1_TTTGCATGAGTAGA-1


title: 10X46_1_TTTGCATGGAATCC-1


title: 10X46_1_TTTCCAGATGCATG-1

value: Radial


title: 10X46_1_TTTCACGATGTAGC-1


title: 10X46_1_TTTCAGTGCCCTCA-1

value: Astrocytes


title: 10X46_1_TTTCACGACTACTT-1


title: 10X46_1_TTTCCAGATAGAGA-1


title: 10X46_1_TTTCCAGACGGAGA-1

value: Granule-immature


title: 10X46_1_TTTCACGACCATAG-1


title: 10X46_1_TTTCACGAGGATCT-1


title: 10X46_1_TTTCACGACTCTCG-1


title: 10X46_1_TTTGACTGATTCGG-1


title: 10X46_1_TTTAGGCTGGAACG-1


title: 10X46_1_TTTATCCTAGAATG-1


title: 10X46_1_TTTAGGCTTTTCAC-1


title: 10X46_1_TTTAGGCTGCAAGG-1


title: 10X46_1_TTTAGGCTCTAGTG-1

value: Endothelial


title: 10X46_1_TTTAGCTGTGTCCC-1


title: 10X46_1_TTTAGCTGCAGAAA-1


title: 10X46_1_TTTAGCTGAGGCGA-1


title: 10X46_1_TTGTAGCTGTCAAC-1


title: 10X46_1_TTGTACACGGTGAG-1


title: 10X46_1_TTGTCATGCCTGTC-1


title: 10X46_1_TTGTACACGCTGAT-1


title: 10X46_1_TTGGTACTGAGACG-1


title: Lung_9M_oxBS_28


title: 10X46_1_TTTAGCTGCTATGG-1


title: 10X46_1_TTGCATTGGGAGTG-1


title: 10X46_1_TTGGAGACGAGGCA-1


title: 10X46_1_TTGGGAACCTCCAC-1


title: 10X46_1_TTGCTAACTTCATC-1


title: 10X46_1_TTGCTATGCCCTAC-1

value: nIPC


title: 10X46_1_TTGGAGTGAAAGTG-1


title: 10X46_1_TTGATCTGCGGGAA-1


title: 10X46_1_TTGAGGTGCCGAAT-1


title: 10X46_1_TTGGAGTGGTTTCT-1


title: 10X46_1_TTGAGGTGCGATAC-1


title: 10X46_1_TTGAGGTGCACTAG-1


title: 10X46_1_TTGAATGATAACGC-1


title: 10X46_1_TTGACACTTGTGGT-1


title: 10X46_1_TTGAATGAGTTGTG-1


title: 10X46_1_TTGAGGACGTACGT-1


title: 10X46_1_TTGAATGACATTCT-1


title: 10X46_1_TTGATCTGAACGTC-1


title: 10X46_1_TTGACACTCAATCG-1


title: 10X46_1_TTGAGGACCCTGAA-1


title: 10X46_1_TTGAATGAGTCAAC-1


title: 10X46_1_TTGACACTTGCAAC-1


title: 10X46_1_TTGAATGAAAGCAA-1


title: 10X46_1_TTGGGAACTCCTCG-1


title: 10X46_1_TTGAACCTCTTAGG-1


title: 10X46_1_TTGGGAACCCAACA-1


title: 10X46_1_TTGATCTGCCTATT-1


title: 10X46_1_TTGGAGTGTACTTC-1


title: 10X46_1_TTCTGATGGTAGGG-1


title: 10X46_1_TTCTCAGAGATGAA-1


title: 10X46_1_TTCTCAGAAGTAGA-1

value: Microglia


title: 10X46_1_TTCTACGAGTACAC-1


title: 10X46_1_TTCTAGTGACAGCT-1


title: 10X46_1_TTCTAGTGCTGAGT-1


title: 10X46_1_TTCGATTGTGCAAC-1


title: 10X46_1_TTCGGAGAAACCTG-1


title: 10X46_1_TTCTACGATTGTCT-1


title: 10X46_1_TTCTAGTGATCAGC-1


title: 10X46_1_TTCGAGGAGTTACG-1


title: 10X46_1_TTCCATGAGGTGGA-1


title: 10X46_1_TTCGTATGAGCAAA-1


title: 10X46_1_TTCCAAACGCGATT-1


title: 10X46_1_TTCTACGACGACAT-1


title: 10X46_1_TTCCATGAAACCGT-1


title: 10X46_1_TTCCATGAACACGT-1


title: 10X46_1_TTCCATGAGTAAAG-1


title: 10X46_1_TTCATCGACTACGA-1


title: 10X46_1_TTCATGTGTGTTCT-1


title: 10X46_1_TTCATGTGCCGCTT-1


title: 10X46_1_TTCCATGACACAAC-1


title: 10X46_1_TTCAGTTGGGTGAG-1


title: 10X46_1_TTCATGTGTCGACA-1


title: 10X46_1_TTCACCCTGGTCTA-1


title: 10X46_1_TTCATGTGAAACAG-1


title: 10X46_1_TTCAGTACGAGGCA-1


title: 10X46_1_TTCAAGCTTTGTGG-1

value: OL


title: 10X46_1_TTCACAACTCCTAT-1


title: 10X46_1_TTCAAAGATGATGC-1


title: 10X46_1_TTCGAGGAGTTTGG-1


title: 10X46_1_TTATTCCTTGGGAG-1


title: 10X46_1_TTATGGCTATTGGC-1


title: 10X46_1_TTATTCCTGTTTGG-1


title: 10X46_1_TTATCCGATTTGCT-1


title: 10X46_1_TTATGAGAGGAGTG-1

value: Neuroblast


title: 10X46_1_TTATGAGAACTAGC-1


title: 10X46_1_TTATGCACTCTAGG-1


title: 10X46_1_TTAGTCACGTCTAG-1


title: 10X46_1_TTAGGGTGGGTTTG-1


title: 10X46_1_TTAGGGTGGAGGTG-1


title: 10X46_1_TTAGGGTGGAATGA-1


title: 10X46_1_TTAGGGTGGTAAAG-1


title: 10X46_1_TTAGGGTGAAGATG-1


title: 10X46_1_TTAGGGACCAGTCA-1


title: 10X46_1_TTAGCTACGGTATC-1


title: 10X46_1_TTAGAATGCCATGA-1


title: 10X46_1_TTAGACCTCCCTAC-1


title: 10X46_1_TTAGGGTGACTAGC-1


title: 10X46_1_TTACTCGACCTTAT-1

value: Cajal-Retzius


title: 10X46_1_TTAGACCTTTCCAT-1


title: 10X46_1_TTCAGTACTACGCA-1


title: 10X46_1_TTACGACTCGTGAT-1


title: 10X46_1_TTAGCTACTGTCCC-1


title: 10X46_1_TTACTCGACGCATA-1


title: 10X46_1_TTACCATGTCCTAT-1


title: 10X46_1_TTACCATGAGCGGA-1


title: 10X46_1_TTACCATGAAGGGC-1


title: 10X46_1_TTACCATGAACAGA-1


title: 10X46_1_TTACAGCTTCTCGC-1


title: 10X46_1_TTAGTCTGTGCATG-1


title: 10X46_1_TTACACACGCAGTT-1


title: 10X46_1_TTAGGTCTCGGGAA-1


title: 10X46_1_TTAACCACGGTAAA-1


title: 10X46_1_TGTTACTGAGCCTA-1


title: 10X46_1_TGTTAAGATGACCA-1


title: 10X46_1_TGTTAAGACGCTAA-1


title: 10X46_1_TGTGAGACCATGCA-1


title: 10X46_1_TGTGATCTTGTCTT-1


title: 10X46_1_TGTGAGTGGGAACG-1


title: 10X46_1_TGTCAGGAAGAGAT-1


title: 10X46_1_TGTCAGGATGCTAG-1


title: 10X46_1_TGTATGCTGACGTT-1


title: 10X46_1_TGTCTAACGTCACA-1


title: 10X46_1_TGTATCTGACTACG-1


title: 10X46_1_TGTAGGTGGTCTTT-1


title: 10X46_1_TGTAGTCTCGCTAA-1


title: 10X46_1_TGTCTAACCTATTC-1


title: 10X46_1_TGTAGGTGGGTGGA-1


title: 10X46_1_TGTAACCTTCTCGC-1


title: 10X46_1_TGTATGCTTCCGTC-1


title: 10X46_1_TGTACTTGGGTGGA-1


title: 10X46_1_TGTAACCTTGTCCC-1


title: 10X46_1_TGTAATGAGTTGTG-1


title: 10X46_1_TGTAAAACGGACTT-1


title: 10X46_1_TGGTCAGAAAAGTG-1


title: 10X46_1_TGGTAGTGGTGAGG-1


title: 10X46_1_TGGTAGACTGAACC-1


title: 10X46_1_TGGTAGTGAGAACA-1


title: 10X46_1_TGTAGGTGCGAACT-1


title: 10X46_1_TGTGACGAATGTCG-1


title: 10X46_1_TGGTAGACGCTATG-1


title: 10X46_1_TGGTAGACGGATCT-1


title: 10X46_1_TGGTACGAATGGTC-1


title: 10X46_1_TGGTATCTGCGATT-1


title: 10X46_1_TGGCACCTTTCAGG-1


title: 10X46_1_TGGCACCTTCCAGA-1


title: 10X46_1_TGGATTCTTGTCCC-1


title: 10X46_1_TGTAGGTGGTTCTT-1


title: 10X46_1_TGGATGACCACTGA-1


title: 10X46_1_TGTAGTCTCCAATG-1


title: 10X46_1_TGGATCGAGAACCT-1


title: 10X46_1_TGGAGGGATAGCCA-1


title: 10X46_1_TGGAGGGACGACTA-1


title: 10X46_1_TGGAGGGAAATCGC-1


title: 10X46_1_TGGAGGGACATCAG-1


title: 10X46_1_TGGACTGAGTGAGG-1


title: 10X46_1_TGGACTGACCGATA-1


title: 10X46_1_TGGACTGAGTTACG-1


title: 10X46_1_TGGAGGGAAAGTAG-1


title: 10X46_1_TGGACCCTACTTTC-1


title: 10X46_1_TGGACCCTGGAGGT-1


title: 10X46_1_TGGAAGCTTACAGC-1


title: 10X46_1_TGGAAGCTCTTACT-1


title: 10X46_1_TGGAACACACCTTT-1


title: 10X46_1_TGCTGAGAAACCTG-1


title: 10X46_1_TGCTAGGATGTCCC-1


title: 10X46_1_TGCTAGGAAGCTCA-1


title: 10X46_1_TGCGTAGAATCTCT-1


title: 10X46_1_TGCGATGAGATGAA-1


title: 10X46_1_TGCCCAACTTCATC-1


title: 10X46_1_TGCTGAGAGGTGAG-1


title: 10X46_1_TGGAGGGATATTCC-1


title: 10X46_1_TGGATGTGGGGACA-1


title: 10X46_1_TGCGAAACCTGCAA-1


title: 10X46_1_TGCCCAACCACTTT-1


title: 10X46_1_TGCGATGAAAGTAG-1


title: 10X46_1_TGCAGATGTCAGAC-1


title: 10X46_1_TGCCAAGAGCGATT-1


title: 10X46_1_TGCCAAGAATGACC-1


title: 10X46_1_TGCAGATGGGACAG-1


title: 10X46_1_TGCCAGCTATGTGC-1


title: 10X46_1_TGCAATCTCTTACT-1


title: 10X46_1_TGCAAGTGAGAGGC-1


title: 10X46_1_TGATCGGAGTAAAG-1


title: 10X46_1_TGATTCTGTGGTCA-1


title: 10X46_1_TGCAACGAACACGT-1


title: 10X46_1_TGATCGGACAGCTA-1


title: 10X46_1_TGATTCACTTGCGA-1


title: 10X46_1_TGATCACTCTCAAG-1


title: 10X46_1_TGATCACTCCGCTT-1


title: 10X46_1_TGATCACTACTACG-1


title: 10X46_1_TGATATGAGTTTCT-1


title: 10X46_1_TGAGTGACAGAACA-1

value: Pericytes


title: 10X46_1_TGATAAACGTCTAG-1


title: 10X46_1_TGAGTGACAACCAC-1


title: 10X46_1_TGATAAACTGCTCC-1


title: 10X46_1_TGAGTCGAGAAACA-1


title: 10X46_1_TGAGTCGAGACAAA-1


title: 10X46_1_TGCCCAACTAAGCC-1


title: 10X46_1_TGAGGACTCCACAA-1


title: 10X46_1_TGAGGTACTTGTCT-1


title: 10X46_1_TGAGGACTAAGGGC-1


title: 10X46_1_TGAGACACCCAGTA-1


title: 10X46_1_TGACTTTGTAAGGA-1


title: 10X46_1_TGAGTCGACCGATA-1


title: 10X46_1_TGACTTACGGTGTT-1


title: 10X46_1_TGACTGGATTCGTT-1


title: 10X46_1_TGACTGGATACAGC-1


title: 10X46_1_TGACTGGAATGTGC-1


title: 10X46_1_TGACCAGAGAGGTG-1


title: 10X46_1_TGACCAGATGCTCC-1


title: 10X46_1_TGACCAGAGCTTAG-1


title: 10X46_1_TGACGATGAAGGGC-1


title: 10X46_1_TGATATGACCTTGC-1


title: 10X46_1_TGAAGCTGCCTACC-1


title: 10X46_1_TGACACGACTGCTC-1


title: 10X46_1_TGAAGCTGAGCTCA-1


title: 10X46_1_TGAGCTGATGTCGA-1


title: 10X46_1_TGAACCGAGGTTAC-1


title: 10X46_1_TGAGCTGAAAGATG-1


title: 10X46_1_TGAACCGAATGCCA-1

value: PVM


title: 10X46_1_TGAACCGACGTGAT-1


title: 10X46_1_TGAAGCTGCTCATT-1


title: 10X46_1_TGAAATTGTCTCAT-1


title: 10X46_1_TGAAGCTGCCGATA-1


title: 10X46_1_TGAAATTGGTGCTA-1


title: 10X46_1_TCTTGATGTGCTTT-1


title: 10X46_1_TCTTGATGTTCACT-1


title: 10X46_1_TCTCAAACACTTTC-1


title: 10X46_1_TCTCAAACACCTCC-1


title: 10X46_1_TCTGATACTGCCTC-1


title: 10X46_1_TCTCTAGAGCAAGG-1


title: 10X46_1_TCTGATACTCCTAT-1


title: 10X46_1_TCTCTAGATGCCTC-1


title: 10X46_1_TCTTACGACTTGGA-1


title: 10X46_1_TCTGATACGGTGTT-1


title: 10X46_1_TGACGCCTGCGTAT-1


title: 10X46_1_TCTCCACTTAAGCC-1


title: 10X46_1_TGACGCCTCAATCG-1


title: 10X46_1_TCTCAAACAAACAG-1


title: 10X46_1_TCTCAAACGTTCAG-1


title: 10X46_1_TCTAAGCTAGCACT-1


title: 10X46_1_TCGTTATGGTGTCA-1


title: 10X46_1_TCGTGAGAGGAACG-1


title: 10X46_1_TCGTTATGGTGTTG-1


title: 10X46_1_TCTAACTGTCGCAA-1


title: 10X46_1_TCGTGAGAGGTGTT-1


title: 10X46_1_TCTAACTGTAAGCC-1


title: 10X46_1_TCGTAGGATGCGTA-1


title: 10X46_1_TCGTAGGACTCTCG-1


title: 10X46_1_TCGTGAGAGTAAGA-1


title: 10X46_1_TCGGCACTTATCTC-1


title: 10X46_1_TGAACCGAACACAC-1


title: 10X46_1_TCTAAGCTTTGCTT-1


title: 10X46_1_TCGCCATGCTGCTC-1


title: 10X46_1_TCGCACTGCCTAAG-1


title: 10X46_1_TCGCACACAGCATC-1


title: 10X46_1_TCGCACTGTGTCCC-1


title: 10X46_1_TCGACGCTCTTGTT-1


title: 10X46_1_TCGAGCCTCGTAGT-1


title: 10X46_1_TCGACGCTGGTATC-1


title: 10X46_1_TCGAGCCTCAGATC-1


title: 10X46_1_TCGACCTGAAGAAC-1


title: 10X46_1_TCGACCTGGAACTC-1


title: 10X46_1_TCGATTTGCAACCA-1


title: 10X46_1_TCGCAAGAATCTCT-1


title: 10X46_1_TCCTATGACGACAT-1


title: 10X46_1_TCGAATCTCGCAAT-1


title: 10X46_1_TCGTAGGAATGACC-1


title: 10X46_1_TCCTACCTTAACGC-1


title: 10X46_1_TCCTATGAACGTTG-1


title: 10X46_1_TCCTACCTGTAGCT-1


title: 10X46_1_TCCCGATGTTTCTG-1


title: 10X46_1_TCCCTACTTCTTAC-1


title: 10X46_1_TCCTACCTACTTTC-1


title: 10X46_1_TCCCGATGCTCGCT-1


title: 10X46_1_TCCCGAACCCTGTC-1


title: 10X46_1_TCCCAGACCCCACT-1


title: 10X46_1_TCGCAAGATACTGG-1


title: 10X46_1_TCGAGCCTTGTCAG-1


title: 10X46_1_TCGAGAACTGAGCT-1


title: 10X46_1_TCCATCCTTTCAGG-1


title: 10X46_1_TCCCACGAGTATCG-1


title: 10X46_1_TCCATCCTATGCTG-1


title: 10X46_1_TCCATAACTGACAC-1


title: 10X46_1_TCCACGTGTTGACG-1


title: 10X46_1_TCCATAACATCGGT-1


title: 10X46_1_TCCAGAGAGCGTAT-1


title: 10X46_1_TCCAGAGAAAAGCA-1


title: 10X46_1_TCCATCCTGAGAGC-1


title: 10X46_1_TCCCGAACCCTTGC-1


title: 10X46_1_TCCAGAGACAGTTG-1


title: 10X46_1_TCCCGATGTATGCG-1


title: 10X46_1_TCCCAGACCATTCT-1


title: 10X46_1_TCCAGAGAAACTGC-1


title: 10X46_1_TCATTGACAAGGGC-1


title: 10X46_1_TCATTGACCATGAC-1


title: 10X46_1_TCAGTTACCTCTAT-1


title: 10X46_1_TCAGTACTAGCTAC-1


title: 10X46_1_TCAGTGGACATTCT-1


title: 10X46_1_TCAGTGGACCCAAA-1


title: 10X46_1_TCATCATGTTGGCA-1


title: 10X46_1_TCAGTTACAAGTGA-1


title: 10X46_1_TCATTCGAATTGGC-1


title: 10X46_1_TCATCAACGTACAC-1


title: 10X46_1_TCATGTACGGAGTG-1


title: 10X46_1_TCATTGACCCTATT-1


title: 10X46_1_TCAGCGCTCCTTCG-1


title: 10X46_1_TCAGCGCTCGTCTC-1


title: 10X46_1_TCAGGATGAAGCAA-1


title: 10X46_1_TCAGCGCTAAGCCT-1


title: 10X46_1_TCAGCGCTCGGAGA-1


title: 10X46_1_TCACTATGAAAGCA-1


title: 10X46_1_TCAGCAGAAAGTGA-1


title: 10X46_1_TCAGCAGACCTACC-1


title: 10X46_1_TCACGAGATCCGAA-1


title: 10X46_1_TCAGAGACACACGT-1


title: 10X46_1_TCATCAACGGTCAT-1


title: 10X46_1_TCACCGTGTCTTTG-1


title: 10X46_1_TCACGAGAGGGTGA-1


title: 10X46_1_TCACATACGGTGGA-1


title: 10X46_1_TCACCGTGGGATCT-1


title: 10X46_1_TCACCCGAGTTTCT-1


title: 10X46_1_TCAAGTCTTCAGTG-1


title: 10X46_1_TCACAACTGGATTC-1


title: 10X46_1_TCAAGGTGTGCTTT-1


title: 10X46_1_TCAAGGTGTGAGAA-1


title: 10X46_1_TCAAGGTGATGCCA-1


title: 10X46_1_TCAAGGTGTCGCCT-1


title: 10X46_1_TATTGCTGCCTGTC-1


title: 10X46_1_TATTGCTGCCTCGT-1


title: 10X46_1_TATGGGTGCCCTCA-1


title: 10X46_1_TCAAGGTGACGACT-1


title: 10X46_1_TATTGCTGTGAACC-1


title: 10X46_1_TATTTCCTCCCACT-1


title: 10X46_1_TCAAGGACTGAACC-1


title: 10X46_1_TATGGGTGACGTAC-1


title: 10X46_1_TATGTCTGTTCCCG-1


title: 10X46_1_TATGTCACCAGTCA-1


title: 10X46_1_TATGAATGTCCTAT-1


title: 10X46_1_TATCTGACACCGAT-1


title: 10X46_1_TATGGGTGTGAGAA-1


title: 10X46_1_TATGGGACTGCAGT-1


title: 10X46_1_TATGCGGACCACCT-1


title: 10X46_1_TATCTCGACATACG-1


title: 10X46_1_TATCGACTCTACTT-1


title: 10X46_1_TATGTGCTGTATCG-1


title: 10X46_1_TATCTCGACGTTGA-1


title: 10X46_1_TATCTCGAACCATG-1


title: 10X46_1_TATCCTGAACGGAG-1


title: 10X46_1_TATCAGCTTCGTTT-1


title: 10X46_1_TATCCAACGAACTC-1


title: 10X46_1_TATAGCCTTCAGGT-1


title: 10X46_1_TCAAGGTGCCCGTT-1


title: 10X46_1_TATCTTCTAGAGAT-1


title: 10X46_1_TATAGATGGGTATC-1


title: 10X46_1_TATAGATGGAATAG-1


title: 10X46_1_TAGTTGCTACGTAC-1


title: 10X46_1_TATACAGACTTTAC-1


title: 10X46_1_TAGTGGTGTATCTC-1


title: 10X46_1_TCACAACTACGTAC-1


title: 10X46_1_TAGTCTTGTTCGTT-1


title: 10X46_1_TATACCACAACGAA-1


title: 10X46_1_TATACAGAAGTGTC-1


title: 10X46_1_TATAAGTGTTGCGA-1


title: 10X46_1_TAGTGGTGGAGAGC-1


title: 10X46_1_TATACCACCAGATC-1


title: 10X46_1_TATACGCTTCCCGT-1


title: 10X46_1_TATAAGACAAAAGC-1


title: 10X46_1_TAGTCTTGGAGGTG-1


title: 10X46_1_TAGTTGCTACCTCC-1


title: 10X46_1_TAGTTAGATCAAGC-1


title: 10X46_1_TATCTCGAGGTGAG-1


title: 10X46_1_TAGTCTTGCAGTCA-1


title: 10X46_1_TAGTCGGAGAATAG-1


title: 10X46_1_TAGTCGGATCGTGA-1


title: 10X46_1_TAGTATGAAGCTAC-1


title: 10X46_1_TAGTACCTCGCATA-1


title: 10X46_1_TAGTAAACGGAGTG-1


title: 10X46_1_TAGTAATGCCCAAA-1


title: 10X46_1_TATAGCCTAAGTAG-1


title: 10X46_1_TAGTCACTTCGATG-1


title: 10X46_1_TAGTACCTACGGAG-1


title: 10X46_1_TAGGTGTGTGCTTT-1


title: 10X46_1_TAGGTTCTCTGTAG-1


title: 10X46_1_TAGGTTCTACCCTC-1


title: 10X46_1_TAGGTGTGCGGTAT-1


title: 10X46_1_TAGGTCGAGCTAAC-1


title: 10X46_1_TAGGCATGAGCAAA-1


title: 10X46_1_TAGGCATGTCTTCA-1


title: 10X46_1_TAGGTCGAAGCCAT-1


title: 10X46_1_TAGGGACTACTAGC-1


title: 10X46_1_TAGGTCGAACGGTT-1


title: 10X46_1_TAGGCATGAGTCTG-1


title: 10X46_1_TAGGTGTGGTATCG-1


title: 10X46_1_TAGGAGCTGGAAAT-1


title: 10X46_1_TAGGCTGAGACAAA-1


title: 10X46_1_TAGGAGCTACTTTC-1


title: 10X46_1_TAGCGATGTCTCCG-1


title: 10X46_1_TAGCTACTAACCGT-1


title: 10X46_1_TAGCGATGTAACGC-1


title: 10X46_1_TAGCGATGGTCTAG-1


title: 10X46_1_TAGGTCGATCCTCG-1


title: 10X46_1_TAGGTGTGGTCAAC-1


title: 10X46_1_TAGCCCACTATCTC-1


title: 10X46_1_TAGCCCTGCTAGAC-1


title: 10X46_1_TAGCCCACGTAGGG-1


title: 10X46_1_TAGCCGCTACGTAC-1


title: 10X46_1_TAGCCCACAGCCAT-1


title: 10X46_1_TAGGGACTTCGACA-1


title: 10X46_1_TAGCCCACCAGAAA-1


title: 10X46_1_TAGCCCACAGATGA-1


title: 10X46_1_TAGGAGCTCCAGTA-1


title: 10X46_1_TAGCATCTGAAAGT-1


title: 10X46_1_TAGCATCTAGATGA-1


title: 10X46_1_TAGATTGACTTTAC-1


title: 10X46_1_TAGATCCTGTGCAT-1


title: 10X46_1_TAGAGCACGGCGAA-1


title: 10X46_1_TAGAGCACTCTCTA-1


title: 10X46_1_TAGAATTGGGTAAA-1


title: 10X46_1_TAGACGTGTGAGCT-1


title: 10X46_1_TAGAATTGTATGCG-1


title: 10X46_1_TAGAATACGCTTCC-1


title: 10X46_1_TAGCCGCTGATAGA-1


title: 10X46_1_TAGCGATGACAGTC-1


title: 10X46_1_TACTTGACCAAGCT-1


title: 10X46_1_TACTTTCTCACTTT-1


title: 10X46_1_TACTTTCTCTGAGT-1


title: 10X46_1_TAGAAACTATCGTG-1


title: 10X46_1_TAGAGAGATAACGC-1


title: 10X46_1_TACTCTGAGAGCAG-1


title: 10X46_1_TAGGTCGAGTAAGA-1


title: 10X46_1_TACTCCCTGGTGGA-1


title: 10X46_1_TACTCAACTTGGTG-1


title: 10X46_1_TACTCCCTATCGGT-1


title: 10X46_1_TACTACTGAAGAAC-1


title: 10X46_1_TACTACACCCAACA-1


title: 10X46_1_TACTCCCTTCTGGA-1


title: 10X46_1_TACTAAGATTTCTG-1


title: 10X46_1_TACGGCCTGCCCTT-1


title: 10X46_1_TACGGCCTCGATAC-1


title: 10X46_1_TACGGCCTACGGGA-1


title: 10X46_1_TACGCGCTGACTAC-1


title: 10X46_1_TACGCCACTGACAC-1


title: 10X46_1_TACGCCACAAGCCT-1


title: 10X46_1_TACGCCACGTCGAT-1


title: 10X46_1_TACTGTTGTACTGG-1


title: 10X46_1_TACGCAGAAAACGA-1


title: 10X46_1_TACTAAGACCTTAT-1


title: 10X46_1_TACGAGTGCCCGTT-1


title: 10X46_1_TACGAGACGGACAG-1


title: 10X46_1_TACGAGTGAAGTAG-1


title: 10X46_1_TAGAAACTCTAGTG-1


title: 10X46_1_TACGACGACTGTCC-1


title: 10X46_1_TACGACGAGCTTCC-1


title: 10X46_1_TACTTTCTCCATGA-1


title: 10X46_1_TACGACGACGACTA-1


title: 10X46_1_TACGACGAGAATCC-1


title: 10X46_1_TACGAGACAGCCTA-1


title: 10X46_1_TACGTACTCCTTGC-1


title: 10X46_1_TACCATTGTCGACA-1


title: 10X46_1_TACCGGCTCTGTTT-1


title: 10X46_1_TACCGCTGTGACTG-1


title: 10X46_1_TACCGAGACTCTAT-1


title: 10X46_1_TACCATTGTGGGAG-1


title: 10X46_1_TACACACTTGTCAG-1


title: 10X46_1_TACGTTACGTGCTA-1


title: 10X46_1_TACACACTTAGAAG-1


title: 10X46_1_TACCATTGCACACA-1


title: 10X46_1_TACACACTGAGGAC-1


title: 10X46_1_TACGCCACTCCTCG-1


title: 10X46_1_TACGAGACCTTCGC-1


title: 10X46_1_TACACACTACTGTG-1


title: 10X46_1_TACAAATGTACAGC-1


title: 10X46_1_TAATGTGACCTGAA-1


title: 10X46_1_TACAATGATCTTCA-1


title: 10X46_1_TAATGTGAAAAAGC-1


title: 10X46_1_TAATGCCTCTTCCG-1


title: 10X46_1_TAATGCCTATTCTC-1


title: 10X46_1_TACAAATGACGTTG-1


title: 10X46_1_TAATGAACGTTGCA-1


title: 10X46_1_TAATGAACCGAACT-1


title: 10X46_1_TAATGAACAGGTTC-1


title: 10X46_1_TAAGGGCTCTAAGC-1


title: 10X46_1_TAAGTAACGTTTGG-1


title: 10X46_1_TAAGTCCTGAGGGT-1


title: 10X46_1_TACATCACGAGACG-1


title: 10X46_1_TAAGCTCTTTTCTG-1


title: 10X46_1_TAAGCTCTAGATCC-1


title: 10X46_1_TAAGGCTGGTAGGG-1


title: 10X46_1_TAAGGGCTCAAAGA-1


title: 10X46_1_TAAGGCTGGCCCTT-1


title: 10X46_1_TAAGCTCTGGAGTG-1


title: 10X46_1_TAAGCGTGATTCCT-1


title: 10X46_1_TAAGCTCTCCCTCA-1


title: 10X46_1_TAAGATTGCTTAGG-1


title: 10X46_1_TAAGAGGAGGACGA-1


title: 10X46_1_TAAGAACTTTGGCA-1


title: 10X46_1_TAAGAGGATCAAGC-1


title: 10X46_1_TAAGCGTGCTCATT-1


title: 10X46_1_TAACCGGAACACGT-1


title: 10X46_1_TAACCGGAATCGGT-1


title: 10X46_1_TAACACCTTGGGAG-1


title: 10X46_1_TAAGAACTCCTACC-1


title: 10X46_1_TAAGAACTCGACAT-1


title: 10X46_1_TAAGGGCTAGAATG-1


title: 10X46_1_TAACTCACTCCAGA-1


title: 10X46_1_TAACGTCTGTTTGG-1


title: 10X46_1_TAACTAGACACTTT-1


title: 10X46_1_TAACGTCTTCACCC-1


title: 10X46_1_TAACAATGACACCA-1


title: 10X46_1_TAACTAGAAACGAA-1


title: 10X46_1_TAAGATTGGGGCAA-1


title: 10X46_1_TAAAGACTTCGACA-1


title: 10X46_1_GTTGAGTGTGCATG-1


title: 10X46_1_GTTGAGTGCGGTAT-1


title: 10X46_1_GTTCATACGTCTTT-1


title: 10X46_1_GTTGTACTTCGTAG-1


title: 10X46_1_TAAATGTGACCACA-1


title: 10X46_1_TAACATGAAAAGCA-1


title: 10X46_1_TAAAGACTCATTCT-1


title: 10X46_1_GTTTAAGACGCATA-1


title: 10X46_1_TAAATCGAGTGTAC-1


title: 10X46_1_GTTTAAGAGACAAA-1


title: 10X46_1_TAAATGTGAAGTGA-1


title: 10X46_1_GTTATGCTCAAAGA-1


title: 10X46_1_GTTGACGAGTCCTC-1


title: 10X46_1_GTTATAGAGGTCAT-1


title: 10X46_1_GTTATCTGGAAGGC-1


title: 10X46_1_GTTAGTCTGAAGGC-1


title: 10X46_1_GTTAGGTGTGGTGT-1


title: 10X46_1_GTTAGTCTGACAGG-1


title: 10X46_1_GTTAGGTGGACTAC-1


title: 10X46_1_TAACAATGGAGCAG-1


title: 10X46_1_GTTATCTGTAGCGT-1


title: 10X46_1_GTTACTACTCAGAC-1


title: 10X46_1_GTTACTACCCCTCA-1


title: 10X46_1_GTTATAGAGAGAGC-1


title: 10X46_1_GTTCATACGAGGCA-1


title: 10X46_1_GTTAACCTAGAATG-1


title: 10X46_1_GTTAACCTCAGCTA-1


title: 10X46_1_GTTACTACATCTCT-1


title: 10X46_1_GTGTGATGAAGAGT-1


title: 10X46_1_GTTACGGATTCTAC-1


title: 10X46_1_GTGTATCTTTTCTG-1


title: 10X46_1_GTTAACCTACGACT-1


title: 10X46_1_GTGTGATGCCAACA-1


title: 10X46_1_GTTACGGACAGCTA-1


title: 10X46_1_GTGTATCTCTACGA-1


title: 10X46_1_GTTAGTCTCCCAAA-1


title: 10X46_1_GTTAAAACGTAAAG-1


title: 10X46_1_GTGTACGAGAACCT-1


title: 10X46_1_GTGTACGACAGTTG-1


title: 10X46_1_GTGGTAACTCGACA-1


title: 10X46_1_GTGTACGACACTGA-1


title: 10X46_1_GTGGTAACGTATCG-1


title: 10X46_1_GTGGTAACGCTAAC-1


title: 10X46_1_GTGGATTGCTCATT-1


title: 10X46_1_GTGCTAGATGGTAC-1


title: 10X46_1_GTTACGGACCTAAG-1


title: 10X46_1_GTGGATTGAGCGTT-1


title: 10X46_1_GTTTAAGATAACGC-1


title: 10X46_1_GTGGATTGAGGGTG-1


title: 10X46_1_GTGCTAGAGCCCTT-1


title: 10X46_1_GTGGATTGGGGCAA-1


title: 10X46_1_GTGCCACTCGCAAT-1


title: 10X46_1_GTGTCAGACACTTT-1


title: 10X46_1_GTGCAAACTGCATG-1


title: 10X46_1_GTGCCACTCATACG-1


title: 10X46_1_GTGATCGAAGGCGA-1

value: OPC


title: 10X46_1_GTGTACGATGCCTC-1


title: 10X46_1_GTGATTCTCTGAGT-1


title: 10X46_1_GTGATTCTTAGTCG-1


title: 10X46_1_GTGATTCTGGGAGT-1


title: 10X46_1_GTGATCGAAATGCC-1


title: 10X46_1_GTGATTCTATCAGC-1


title: 10X46_1_GTGACCCTCACTCC-1


title: 10X46_1_GTGAGGGATACGCA-1


title: 10X46_1_GTGACAACCATCAG-1


title: 10X46_1_GTCTGAGACCATAG-1


title: 10X46_1_GTGAACACCCATAG-1


title: 10X46_1_GTGACAACACCTAG-1


title: 10X46_1_GTCCACTGGCAGAG-1

value: VLMC


title: 10X46_1_GTCCCATGGTTCAG-1


title: 10X46_1_GTCGAATGTCGTAG-1


title: 10X46_1_GTCCAAGATGTGGT-1


title: 10X46_1_GTCCACTGACGTGT-1


title: 10X46_1_GTGCAAACGAAACA-1


title: 10X46_1_GTCACAGAGACGTT-1


title: 10X46_1_GTCACAGACCAAGT-1


title: 10X46_1_GTCAATCTTTTGTC-1


title: 10X46_1_GTCAATCTACTAGC-1


title: 10X46_1_GTCTAGGATGCAGT-1


title: 10X46_1_GTAGTGTGGGTCTA-1


title: 10X46_1_GTATCTACGACTAC-1


title: 10X46_1_GTAGTGTGGTATCG-1


title: 10X46_1_GTATCACTGTGTAC-1


title: 10X46_1_GTAGTGTGCCGAAT-1


title: 10X46_1_GTATTAGATGGTCA-1


title: 10X46_1_GTGTAGTGTGCATG-1


title: 10X46_1_GTATCTACGCTAAC-1


title: 10X46_1_GTCTAACTACCAGT-1


title: 10X46_1_GTAGTGACTGTGCA-1


title: 10X46_1_GTAGTGACAAGATG-1


title: 10X46_1_GTCACCTGACGGTT-1


title: 10X46_1_GTAGGTACACACTG-1


title: 10X46_1_GTCCACACGCGTAT-1


title: 10X46_1_GTCACCTGTCCTAT-1


title: 10X46_1_GTAGTCGATACGAC-1


title: 10X46_1_GTAGCATGGGTACT-1


title: 10X46_1_GTAGCCCTCCTATT-1


title: 10X46_1_GTAGACTGTTTGTC-1


title: 10X46_1_GTCAATCTTGTTCT-1


title: 10X46_1_GTAGCCCTGTACGT-1


title: 10X46_1_GTAGACTGTCTATC-1


title: 10X46_1_GTACTTTGGGGATG-1


title: 10X46_1_GTACTTTGACGGAG-1


title: 10X46_1_GTACTTTGTATCGG-1


title: 10X46_1_GTACTACTAAGGGC-1


title: 10X46_1_GTACGTGAGGCAAG-1


title: 10X46_1_GTACGTGAAGAGGC-1


title: 10X46_1_GTACAGTGTCTTTG-1


title: 10X46_1_GTACCCTGTTCTTG-1


title: 10X46_1_GTACGAACGTGAGG-1


title: 10X46_1_GTACAGTGGAGGTG-1


title: 10X46_1_GTAATAACTTCATC-1


title: 10X46_1_GTAAGCTGTGTCCC-1


title: 10X46_1_GTAGCAACCTCAAG-1


title: 10X46_1_GTACCCTGAAGCCT-1


title: 10X46_1_GTACAGTGCCACAA-1


title: 10X46_1_GTAAGCACGAGGAC-1


title: 10X46_1_GTAAGCTGTGGCAT-1


title: 10X46_1_GTAAGCACCTATTC-1


title: 10X46_1_GTAAGCACAACTGC-1


title: 10X46_1_GTAAGCACAACAGA-1


title: 10X46_1_GGTTTACTGAGGAC-1


title: 10X46_1_GTAACGTGTTGGCA-1


title: 10X46_1_GTAACGTGTTGCAG-1


title: 10X46_1_GGTTTACTAGCAAA-1


title: 10X46_1_GGTTTACTGGTGTT-1


title: 10X46_1_GGTTTACTACGGAG-1


title: 10X46_1_GTATTCACGGTAAA-1


title: 10X46_1_GGTTTACTAACCGT-1


title: 10X46_1_GGTTGAACTTACCT-1


title: 10X46_1_GTAACGTGCATTTC-1


title: 10X46_1_GGTGATACGCTAAC-1


title: 10X46_1_GGTCTAGAAGTGCT-1


title: 10X46_1_GGTATGACGACGTT-1


title: 10X46_1_GGTATGACCTTAGG-1


title: 10X46_1_GGTAGTACCTTGTT-1


title: 10X46_1_GGTATCGACCTTGC-1


title: 10X46_1_GGTATGACACACCA-1


title: 10X46_1_GGTACATGACGTGT-1


title: 10X46_1_GGTACATGTACTGG-1


title: 10X46_1_GGTACAACTCCTAT-1


title: 10X46_1_GGTGATACACGGAG-1


title: 10X46_1_GGTAAAGACCTACC-1


title: 10X46_1_GGGTTAACAAGAGT-1


title: 10X46_1_GGGTTATGTTACCT-1


title: 10X46_1_GGGCCAACCCACAA-1


title: 10X46_1_GGGTAACTCTCGCT-1


title: 10X46_1_GGGTTAACTGAGGG-1


title: 10X46_1_GGGCAGCTAACGTC-1


title: 10X46_1_GGTAAAGACCGATA-1


title: 10X46_1_GGGCCATGCTTGCC-1


title: 10X46_1_GGGCCATGAGCAAA-1


title: 10X46_1_GGGCAGCTCTATTC-1


title: 10X46_1_GGGCACACGTAAAG-1


title: 10X46_1_GGGCACACATCGTG-1


title: 10X46_1_GGTTGAACCTACCC-1


title: 10X46_1_GGGCAAGAGGCATT-1


title: 10X46_1_GGGATTACTTTGGG-1


title: 10X46_1_GGGCAAGATAGAGA-1


title: 10X46_1_GGGATTACAAGCCT-1


title: 10X46_1_GGGATGGAGGGTGA-1


title: 10X46_1_GGTATCGAATCGGT-1


title: 10X46_1_GGGACCACGTACCA-1


title: 10X46_1_GGGACCTGATGTGC-1


title: 10X46_1_GGGACCTGTCACCC-1


title: 10X46_1_GGGACCACAAGGCG-1


title: 10X46_1_GGGACCACAACAGA-1


title: 10X46_1_GGGACCACACTGTG-1


title: 10X46_1_GGGAAGTGTTGTGG-1


title: 10X46_1_GGGAAGTGTTATCC-1


title: 10X46_1_GGGAAGACTGTGGT-1


title: 10X46_1_GGGAACGATGGAGG-1


title: 10X46_1_GGGAACGACTTGTT-1


title: 10X46_1_GGGAACGACTTAGG-1


title: 10X46_1_GGGAACGATGCACA-1


title: 10X46_1_GGCTCACTGTACAC-1


title: 10X46_1_GGCTAATGCAGCTA-1


title: 10X46_1_GGGAACGAAGCTCA-1


title: 10X46_1_GGCGGACTGGAGCA-1


title: 10X46_1_GGCGCATGTTTCAC-1


title: 10X46_1_GGCTAATGAACGAA-1


title: 10X46_1_GGCGCATGTGAACC-1


title: 10X46_1_GGCGGACTTATCGG-1


title: 10X46_1_GGCGACTGTCTATC-1


title: 10X46_1_GGCGACTGGTTAGC-1


title: 10X46_1_GGCGACTGCTTATC-1


title: 10X46_1_GGGCCATGAAGAGT-1


title: 10X46_1_GGGATGGAGGAGCA-1


title: 10X46_1_GTGACAACGGAACG-1


title: 10X46_1_GGCGACACGTGAGG-1


title: 10X46_1_GGCGACTGACACAC-1


title: 10X46_1_GGCCGATGCAGTTG-1


title: 10X46_1_GGCGACACGGCAAG-1


title: 10X46_1_GGCCGATGCGTCTC-1


title: 10X46_1_GGCCGAACTTCACT-1


title: 10X46_1_GGCCCAGATATTCC-1


title: 10X46_1_GGCCAGACTGGCAT-1


title: 10X46_1_GGCCAGACGATACC-1


title: 10X46_1_GGCCAGACATCGAC-1


title: 10X46_1_GGCCACGAGTTCTT-1


title: 10X46_1_GGCCACGATAGTCG-1


title: 10X46_1_GGCAAGGAGAGATA-1


title: 10X46_1_GGCACGTGCAACTG-1


title: 10X46_1_GGCCAGACCTATTC-1


title: 10X46_1_GGATTTCTTGAACC-1


title: 10X46_1_GGATTGTGTGTCCC-1


title: 10X46_1_GGCAATACGTACCA-1


title: 10X46_1_GGCAAGGAGACGTT-1


title: 10X46_1_GGATTGTGACTAGC-1


title: 10X46_1_GGCAAGGATGCCAA-1


title: 10X46_1_GGATGTTGCGACTA-1


title: 10X46_1_GGATTTCTGACACT-1


title: 10X46_1_GGATGTACTCTTAC-1


title: 10X46_1_GGATGTACAGGAGC-1


title: 10X46_1_GGATGTACGACGAG-1


title: 10X46_1_GGATAGCTCAGAGG-1


title: 10X46_1_GGCCCAGAGGACGA-1


title: 10X46_1_GGAGTTTGGCATCA-1


title: 10X46_1_GGCCCAGATTCACT-1


title: 10X46_1_GGCCAGACGTTCTT-1


title: 10X46_1_GGATAGCTATCGGT-1


title: 10X46_1_GGCATATGCACTAG-1


title: 10X46_1_GGAGGTGATCAAGC-1


title: 10X46_1_GGAGGTGAAATGCC-1


title: 10X46_1_GGAGGCCTCTCATT-1


title: 10X46_1_GGAGGCCTCGCAAT-1


title: 10X46_1_GGAGGATGGACAAA-1


title: 10X46_1_GGAGCGCTCTGATG-1


title: 10X46_1_GGAGCGCTACGGTT-1


title: 10X46_1_GGAGCCACCAAGCT-1


title: 10X46_1_GGAGCCACCGAGTT-1


title: 10X46_1_GGAGAGACCTTAGG-1


title: 10X46_1_GGAGAGACGACGAG-1


title: 10X46_1_GGACGCTGCCTTGC-1


title: 10X46_1_GGAGAGACAACTGC-1


title: 10X46_1_GGAGAGACAGAGTA-1


title: 10X46_1_GGACGCTGCTCAAG-1


title: 10X46_1_GGATGTACGGAGCA-1


title: 10X46_1_GGACGCTGCCTACC-1


title: 10X46_1_GGACGCTGCGGTAT-1


title: 10X46_1_GGACGAGAAAGAGT-1


title: 10X46_1_GGACGCTGACCCAA-1


title: 10X46_1_GGACGCTGAGAGAT-1


title: 10X46_1_GGACCGTGTGTGAC-1


title: 10X46_1_GGACATTGTAGTCG-1


title: 10X46_1_GGACATTGGGTGAG-1


title: 10X46_1_GGACATTGACGTGT-1


title: 10X46_1_GGCGCATGCACTTT-1


title: 10X46_1_GGAAGGTGCTTCTA-1


title: 10X46_1_GGACAGGATAGCGT-1


title: 10X46_1_GGACAGGAAGCATC-1


title: 10X46_1_GGACAACTGAGGAC-1


title: 10X46_1_GGAGCAGAACCCTC-1


title: 10X46_1_GGAAGGACTTCTAC-1


title: 10X46_1_GGAACTTGACTGTG-1


title: 10X46_1_GGAAGGACACGGAG-1


title: 10X46_1_GGAGGATGACCAGT-1


title: 10X46_1_GGAACTTGGAGCAG-1


title: 10X46_1_GCTTGAGAGACGAG-1


title: 10X46_1_GGAACTACTGGAGG-1


title: 10X46_1_GGAACTACTGAACC-1


title: 10X46_1_GCTTGAGATGTGGT-1


title: 10X46_1_GGAACTACGCGTAT-1


title: 10X46_1_GGACGCACTCATTC-1


title: 10X46_1_GGAACACTGTGAGG-1


title: 10X46_1_GCTCGACTGTCCTC-1


title: 10X46_1_GCTTGAGAAACGTC-1


title: 10X46_1_GCTTGAGAGACAGG-1


title: 10X46_1_GCTCGACTCGAGAG-1


title: 10X46_1_GCTCCATGTGATGC-1


title: 10X46_1_GGACATTGGAGGAC-1


title: 10X46_1_GCTGATGAGCGAGA-1


title: 10X46_1_GGAAGGTGTTAGGC-1


title: 10X46_1_GCTCAGCTTTTCAC-1


title: 10X46_1_GCTCCATGAGTCTG-1


title: 10X46_1_GCTCACTGTGTGCA-1


title: 10X46_1_GCTCAGCTGTTGCA-1


title: 10X46_1_GCTCAAGACTGATG-1


title: 10X46_1_GCTCAAGATGAAGA-1


title: 10X46_1_GCTCAGCTAGAACA-1


title: 10X46_1_GCTATACTTAGACC-1


title: 10X46_1_GCTATACTGCCAAT-1


title: 10X46_1_GCTCAAGAAACGGG-1


title: 10X46_1_GCTCAAGAGGTCAT-1


title: 10X46_1_GCTACAGATAACCG-1


title: 10X46_1_GCGTATGAACAGTC-1


title: 10X46_1_GCGTACCTTTTCGT-1


title: 10X46_1_GCTACCTGTGAGGG-1


title: 10X46_1_GCTACAGAGTTGGT-1


title: 10X46_1_GCTACCTGAAGAGT-1


title: 10X46_1_GCGTACCTCGAGTT-1


title: 10X46_1_GCGGGACTGGAAAT-1


title: 10X46_1_GCGGGACTTGCAAC-1


title: 10X46_1_GCGGCAACTTCTAC-1


title: 10X46_1_GCTAGATGGTTTCT-1


title: 10X46_1_GCGTACCTCGCTAA-1


title: 10X46_1_GCGTATGACCATAG-1


title: 10X46_1_GCGTATGAGTGTCA-1


title: 10X46_1_GCGTACCTCCAGTA-1


title: 10X46_1_GCGGAGCTTCAGAC-1


title: 10X46_1_GCGGAGCTACCTGA-1


title: 10X46_1_GCGCGATGATCAGC-1


title: 10X46_1_GCGCATCTACCACA-1


title: 10X46_1_GCGCGAACTCTCGC-1


title: 10X46_1_GCGCGAACGTTGAC-1


title: 10X46_1_GCGCATCTAGGGTG-1


title: 10X46_1_GCGCACGAGTGAGG-1


title: 10X46_1_GCGAAGGACTTGCC-1


title: 10X46_1_GCCTGACTATGGTC-1


title: 10X46_1_GCGACTCTCGACAT-1


title: 10X46_1_GCGATATGCGGAGA-1


title: 10X46_1_GCGACTCTCTGTCC-1


title: 10X46_1_GCGGGACTCATTTC-1


title: 10X46_1_GCCTGACTAGTTCG-1


title: 10X46_1_GCCTCATGAGAGAT-1


title: 10X46_1_GCCTCAACGGGAGT-1


title: 10X46_1_GCCTAGCTTTGACG-1


title: 10X46_1_GCCTAGCTTTCAGG-1


title: 10X46_1_GCCTACACGGGAGT-1


title: 10X46_1_GCCTACACGATAAG-1


title: 10X46_1_GCCGTACTAACGTC-1


title: 10X46_1_GCCGGAACGTCAAC-1


title: 10X46_1_GCCTAGCTGGTCAT-1


title: 10X46_1_GCCGAGTGTTCGTT-1


title: 10X46_1_GCCGACGATGTCCC-1


title: 10X46_1_GCCGAGTGCAATCG-1


title: 10X46_1_GCCGACGATAACGC-1


title: 10X46_1_GCCCAGGAACTCTT-1


title: 10X46_1_GCCCAACTGAGAGC-1


title: 10X46_1_GCCCAACTTTCTAC-1


title: 10X46_1_GCCATGCTTGCTCC-1


title: 10X46_1_GCCATGCTGAGCTT-1


title: 10X46_1_GCCATGCTAGTGCT-1


title: 10X46_1_GCCTAGCTACGTAC-1


title: 10X46_1_GCCACTACTCGCTC-1


title: 10X46_1_GCCATCACGTTTGG-1


title: 10X46_1_GCCATGCTAGCTCA-1


title: 10X46_1_GCCACGGAACAGCT-1


title: 10X46_1_GCCAAAACGTCATG-1


title: 10X46_1_GCCGAGTGCCCTTG-1


title: 10X46_1_GCATTGGAAAGGCG-1


title: 10X46_1_GCCAAAACACACGT-1


title: 10X46_1_GCATGTGATTCATC-1


title: 10X46_1_GCATTGGATCTCGC-1


title: 10X46_1_GCCAAATGTAACCG-1


title: 10X46_1_GCCAACCTGGAACG-1


title: 10X46_1_GCCGAGTGCGATAC-1


title: 10X46_1_GCATTGGAAGTCGT-1


title: 10X46_1_GCATGTGAATACCG-1


title: 10X46_1_GCATGTGAACGTTG-1


title: 10X46_1_GCATGTGAGGAAGC-1


title: 10X46_1_GCCCATACAGAAGT-1


title: 10X46_1_GCATCAGATTCGTT-1


title: 10X46_1_GCATGATGGAGGGT-1


title: 10X46_1_GGAAGGACTTGCGA-1


title: 10X46_1_GCATGATGTTGGTG-1


title: 10X46_1_GCAGGCACTTGCGA-1


title: 10X46_1_GCCAAAACCGACAT-1


title: 10X46_1_GCATCAGATCTATC-1


title: 10X46_1_GCATCAGACACAAC-1


title: 10X46_1_GCAGGGCTGGGAGT-1


title: 10X46_1_GCAGTCCTTTCTAC-1


title: 10X46_1_GCAGTCCTAGTCTG-1


title: 10X46_1_GCATCAGAAATCGC-1


title: 10X46_1_GCAGGCACCCACCT-1


title: 10X46_1_GCAGCTCTGGGAGT-1


title: 10X46_1_GCAGTTGATGCAGT-1


title: 10X46_1_GCAGCGTGACGGAG-1


title: 10X46_1_GCAGCTCTCTATTC-1


title: 10X46_1_GCAGCTCTGAGCTT-1


title: 10X46_1_GCACTGCTGTGCTA-1


title: 10X46_1_GCCAAAACCAGAAA-1


title: 10X46_1_GCAGCTCTTGACTG-1


title: 10X46_1_GCAGCCGACGGAGA-1


title: 10X46_1_GCACTGCTGCAGTT-1


title: 10X46_1_GCACTGCTCGACAT-1


title: 10X46_1_GCACTAGATAAAGG-1


title: 10X46_1_GCACTAGAGGAGTG-1


title: 10X46_1_GCACGTCTCGACTA-1


title: 10X46_1_GCACGGTGTCTACT-1


title: 10X46_1_GCACGTCTATCGGT-1


title: 10X46_1_GCACGGACGTAGCT-1


title: 10X46_1_GCACCTTGTTACTC-1


title: 10X46_1_GCACCTACAGGCGA-1


title: 10X46_1_GCCTCAACTCCTGC-1


title: 10X46_1_GCACAATGGCCTTC-1


title: 10X46_1_GCAATCGATCGTAG-1


title: 10X46_1_GCAATTCTCCACAA-1


title: 10X46_1_GCAGATACACGGAG-1


title: 10X46_1_GCAATCGAACGGAG-1


title: 10X46_1_GCAATCGAGGTGTT-1


title: 10X46_1_GCAAGACTGTACGT-1


title: 10X46_1_GCAAGACTCCAGTA-1


title: 10X46_1_GCAACTGACACACA-1


title: 10X46_1_GCAACTGACTCAGA-1


title: 10X46_1_GATTTGCTCTGGTA-1


title: 10X46_1_GCAAACTGGCAGTT-1


title: 10X46_1_GATTTGCTGGAAAT-1


title: 10X46_1_GATTTGCTCGACAT-1


title: 10X46_1_GCACGTCTCGGAGA-1


title: 10X46_1_GATTGGTGCCTATT-1


title: 10X46_1_GATTGGACGTTCGA-1


title: 10X46_1_GATTGGTGGGTCTA-1


title: 10X46_1_GCACCACTGTTCTT-1


title: 10X46_1_GATTGGACAGCGGA-1


title: 10X46_1_GCACCTTGACGACT-1


title: 10X46_1_GATTCTTGAGGAGC-1


title: 10X46_1_GATTCTTGCTTATC-1


title: 10X46_1_GATTACCTGAGATA-1


title: 10X46_1_GCACAATGGCTTAG-1


title: 10X46_1_GCACAATGTGTTTC-1


title: 10X46_1_GATTCTACAGAGGC-1


title: 10X46_1_GCACAATGATCGAC-1


title: 10X46_1_GATGCCCTGAGGCA-1


title: 10X46_1_GATTTAGATAGAGA-1


title: 10X46_1_GATGCATGTCAAGC-1


title: 10X46_1_GATGCATGGAATAG-1


title: 10X46_1_GATGACACTTATCC-1


title: 10X46_1_GATGCCCTATGTCG-1


title: 10X46_1_GATGCATGTTTGGG-1


title: 10X46_1_GATCTTTGGTATCG-1


title: 10X46_1_GATCTTTGACTACG-1


title: 10X46_1_GATCTTTGTAAGGA-1


title: 10X46_1_GATCTTTGATGTGC-1


title: 10X46_1_GATCTTACTCCTGC-1


title: 10X46_1_GATCTTTGATCTTC-1


title: 10X46_1_GATCTTTGGCTTCC-1


title: 10X46_1_GATCTTACCTATGG-1


title: 10X46_1_GATCGTGAGAGCTT-1


title: 10X46_1_GATCGTGAGTAGGG-1


title: 10X46_1_GATCGTGACTCGAA-1


title: 10X46_1_GATCGTGACAGTCA-1


title: 10X46_1_GATCCGCTTCGCCT-1


title: 10X46_1_GATCCGCTTCTATC-1


title: 10X46_1_GATCGATGTGCATG-1


title: 10X46_1_GATCCGCTCGAACT-1


title: 10X46_1_GATCATCTGGTTCA-1


title: 10X46_1_GATCGAACTGAACC-1


title: 10X46_1_GATGCAACAGGTCT-1


title: 10X46_1_GATATTGAACTAGC-1


title: 10X46_1_GATATTGAAACAGA-1


title: 10X46_1_GATATTGAGACGAG-1


title: 10X46_1_GATCCCTGGTAGCT-1


title: 10X46_1_GATATCCTAATCGC-1


title: 10X46_1_GATATCCTCAAAGA-1


title: 10X46_1_GATATATGGCTTCC-1


title: 10X46_1_GATATCCTTTCGCC-1


title: 10X46_1_GATATAACCTATGG-1


title: 10X46_1_GATAGCACTTATCC-1


title: 10X46_1_GATAGCACGTGCTA-1


title: 10X46_1_GATAGAGAGAATGA-1


title: 10X46_1_GATAGAGAGACGAG-1


title: 10X46_1_GATAGAGAAGGGTG-1


title: 10X46_1_GATAGAGAATGCTG-1


title: 10X46_1_GATAATACGTATGC-1


title: 10X46_1_GATACTCTACCAGT-1


title: 10X46_1_GAGTTGTGGCTAAC-1


title: 10X46_1_GATAAGGAGGCAAG-1


title: 10X46_1_GATAATACCCCAAA-1


title: 10X46_1_GAGTGTTGCTGAGT-1


title: 10X46_1_GAGTGACTCAGGAG-1


title: 10X46_1_GAGTCAACGATAAG-1


title: 10X46_1_GAGTCTGATCGACA-1


title: 10X46_1_GAGTGGGAAGCAAA-1


title: 10X46_1_GAGTCTGACGGAGA-1


title: 10X46_1_GAGTACTGCTCTCG-1


title: 10X46_1_GAGTACACCGTGAT-1


title: 10X46_1_GAGTACTGTCTTTG-1


title: 10X46_1_GAGTACTGATTCGG-1


title: 10X46_1_GAGTCTGACACTTT-1


title: 10X46_1_GAGTAAGAAGTCTG-1


title: 10X46_1_GAGGTTTGCATTTC-1


title: 10X46_1_GAGTACACACGACT-1


title: 10X46_1_GAGTAAGACCTGAA-1


title: 10X46_1_GATAATACTCGCCT-1


title: 10X46_1_GAGGTGGAGCAAGG-1


title: 10X46_1_GAGGGCCTGTTAGC-1


title: 10X46_1_GAGTAAGACGCATA-1


title: 10X46_1_GAGGGTGATTTGCT-1


title: 10X46_1_GAGGGCCTAACAGA-1


title: 10X46_1_GAGGGAACAGTGCT-1


title: 10X46_1_GAGGCCACACTTTC-1


title: 10X46_1_GAGGCAGAATCAGC-1


title: 10X46_1_GAGGCAGAATAAGG-1


title: 10X46_1_GAGGCAGACTTCGC-1


title: 10X46_1_GAGGCAGATCAGGT-1


title: 10X46_1_GAGGATCTTCTCGC-1


title: 10X46_1_GAGGATCTTAGCCA-1


title: 10X46_1_GAGGATCTGTTCAG-1


title: 10X46_1_GAGGATCTAGTCGT-1


title: 10X46_1_GAGTACTGATCACG-1


title: 10X46_1_GAGCTCCTTAGCCA-1


title: 10X46_1_GAGCTCCTCTCGCT-1


title: 10X46_1_GAGCTCCTGACTAC-1


title: 10X46_1_GAGCTCCTGTTTGG-1


title: 10X46_1_GAGCTCCTGTGCAT-1


title: 10X46_1_GAGCGGCTGTCACA-1


title: 10X46_1_GAGCGCACAGCATC-1


title: 10X46_1_GAGCGGCTGCGTAT-1


title: 10X46_1_GAGCGAGATTTGTC-1


title: 10X46_1_GAGCGCTGAAGCAA-1


title: 10X46_1_GAGCGAGATTGTGG-1


title: 10X46_1_GAGGGTGACGCCTT-1


title: 10X46_1_GAGCAACTTAGCGT-1


title: 10X46_1_GAGCAACTAAAGCA-1


title: 10X46_1_GAGATCACATGTCG-1


title: 10X46_1_GAGCAGGACCTAAG-1


title: 10X46_1_GAGGGATGATGCCA-1


title: 10X46_1_GAGATAGATGGGAG-1


title: 10X46_1_GAGATAGAAGATCC-1


title: 10X46_1_GAGAAATGTGGTCA-1


title: 10X46_1_GAGAAATGTCCTAT-1


title: 10X46_1_GACTTTACTTGGCA-1


title: 10X46_1_GAGGATCTTCTGGA-1


title: 10X46_1_GACTTTACTGATGC-1


title: 10X46_1_GACTGTGAAGTGTC-1


title: 10X46_1_GACTGTGATACTGG-1


title: 10X46_1_GACTACGAGTAGGG-1


title: 10X46_1_GACTGAACGAGGTG-1


title: 10X46_1_GACGTCCTTGATGC-1


title: 10X46_1_GACTACGAAGCAAA-1


title: 10X46_1_GACTGATGCATTGG-1


title: 10X46_1_GACTGATGAGCATC-1


title: 10X46_1_GACTGTGACCTCCA-1


title: 10X46_1_GACTACGACAGTCA-1


title: 10X46_1_GACGTAACGTTACG-1


title: 10X46_1_GACTGAACTTTGTC-1


title: 10X46_1_GACTGTGACTGCAA-1


title: 10X46_1_GACGTCCTCTTATC-1


title: 10X46_1_GACGTCCTGTTGAC-1


title: 10X46_1_GACGCCGACGAGAG-1


title: 10X46_1_GACGCTCTTTCACT-1


title: 10X46_1_GACGCCGACTGTTT-1


title: 10X46_1_GACGGCACAACCTG-1


title: 10X46_1_GAGAAATGGCGATT-1


title: 10X46_1_GACGATTGACGTTG-1


title: 10X46_1_GACGATTGATCTCT-1


title: 10X46_1_GACGATTGACGGGA-1


title: 10X46_1_GACTTTACCCGCTT-1


title: 10X46_1_GACGATTGATGCCA-1


title: 10X46_1_GACTTTACTAGAGA-1


title: 10X46_1_GACGAGGACCAAGT-1


title: 10X46_1_GACGAACTGTCCTC-1


title: 10X46_1_GACCTCTGCTTGCC-1


title: 10X46_1_GATGACACTCAAGC-1


title: 10X46_1_GACCTCTGCCTATT-1


title: 10X46_1_GACCAAACGGTAGG-1


title: 10X46_1_GACCAAACTCAAGC-1


title: 10X46_1_GACGGCACTGAACC-1


title: 10X46_1_GACCATGACAGAAA-1


title: 10X46_1_GACCTAGACAGATC-1


title: 10X46_1_GACCATGAAGTGTC-1


title: 10X46_1_GACCTCTGACCTTT-1


title: 10X46_1_GACAGTACTCTTTG-1


title: 10X46_1_GACATTCTAGCAAA-1


title: 10X46_1_GACCAAACGAGAGC-1


title: 10X46_1_GACATTCTTATCGG-1


title: 10X46_1_GACAGTACGTAGCT-1


title: 10X46_1_GACAGTACAAACGA-1


title: 10X46_1_GACAACTGTGCAGT-1


title: 10X46_1_GACAACTGTTGACG-1


title: 10X46_1_GACAACTGGGGATG-1


title: 10X46_1_GAATGCTGGCTAAC-1


title: 10X46_1_GAATGCACTGACTG-1


title: 10X46_1_GAATGCACGTGTAC-1


title: 10X46_1_GACAACTGTCGTAG-1


title: 10X46_1_GACAACTGTATCTC-1


title: 10X46_1_GAATGCACAAACAG-1


title: 10X46_1_GACAGTTGTTGTGG-1


title: 10X46_1_GAAGTGCTTCTTCA-1


title: 10X46_1_GAATGCACTGGGAG-1


title: 10X46_1_GAAGGTCTAAGAGT-1


title: 10X46_1_GAAGGTCTAGCTAC-1


title: 10X46_1_GAAGTGCTCCACAA-1


title: 10X46_1_GAAGGGTGCCCACT-1


title: 10X46_1_GAAGTCTGCGTAGT-1


title: 10X46_1_GAAGGGTGGGTAGG-1


title: 10X46_1_GAAGCTTGGGTTCA-1


title: 10X46_1_GACATTCTATGACC-1


title: 10X46_1_GAAGCTTGTTTGCT-1


title: 10X46_1_GAAGTCACCGCTAA-1


title: 10X46_1_GACAGGGAGGACAG-1


title: 10X46_1_GAAGCTACTTACTC-1


title: 10X46_1_GAAGTCACATGCCA-1


title: 10X46_1_GAAGTGCTCCGCTT-1


title: 10X46_1_GAATGGCTTGGAAA-1


title: 10X46_1_GAAGCGGAGGTATC-1


title: 10X46_1_GAAGCTACAAGAAC-1


title: 10X46_1_GAACGGGACAGATC-1


title: 10X46_1_GAACCTGATACGAC-1


title: 10X46_1_GAACCTGACCTCGT-1


title: 10X46_1_GAACCAACTCAGTG-1


title: 10X46_1_GAACCAACCGAACT-1


title: 10X46_1_GAACCAACTGCTGA-1


title: 10X46_1_GAAGAATGGTCAAC-1


title: 10X46_1_GAACCAACCAACCA-1


title: 10X46_1_GAACCAACATTCGG-1


title: 10X46_1_GAAGTGCTGGTGGA-1


title: 10X46_1_GAACCAACAGCTAC-1


title: 10X46_1_GAACAGCTCTGATG-1


title: 10X46_1_GAACAGCTTGACTG-1


title: 10X46_1_GAACACACCCAATG-1


title: 10X46_1_GAACAGCTTCTTCA-1


title: 10X46_1_GAAATACTTTCACT-1


title: 10X46_1_GAACCAACATCTTC-1


title: 10X46_1_GAAATACTGGAAGC-1


title: 10X46_1_GAAATACTGTCTGA-1


title: 10X46_1_GAAAGTGATGTGCA-1


title: 10X46_1_GAAATACTGCCTTC-1


title: 10X46_1_GAACGGGACTTACT-1


title: 10X46_1_GAAAGCCTTGCCTC-1


title: 10X46_1_GAAATACTGAATAG-1


title: 10X46_1_GAAAGATGGGCATT-1


title: 10X46_1_GAAAGTGACGTACA-1


title: 10X46_1_GAAAGTGATGCTTT-1


title: 10X46_1_GAAACCTGCACCAA-1


title: 10X46_1_GAAAGTGAGTAGCT-1


title: 10X46_1_CTTTCAGATTGACG-1


title: 10X46_1_CTTTCAGACTGCAA-1


title: 10X46_1_CTTTCAGAAGGCGA-1


title: 10X46_1_CTTTGATGGGAAAT-1


title: 10X46_1_CTTTCAGAGTTCGA-1


title: 10X46_1_GAAACAGATCCCAC-1


title: 10X46_1_CTTTAGTGTTACCT-1


title: 10X46_1_CTTTAGACGTTACG-1


title: 10X46_1_CTTTAGACGGAGTG-1


title: 10X46_1_GAACACACTGCCCT-1


title: 10X46_1_CTTTACGAGTACAC-1


title: 10X46_1_CTTTACGACTAGAC-1


title: 10X46_1_CTTTACGAAGCAAA-1


title: 10X46_1_CTTGATTGTGTCCC-1


title: 10X46_1_GAAAGATGAAAGTG-1


title: 10X46_1_CTTGAGGATGAAGA-1


title: 10X46_1_CTTGAGGAATCGAC-1


title: 10X46_1_CTTCTAGAGTAGCT-1


title: 10X46_1_CTTCTAGACCTGTC-1


title: 10X46_1_CTTGAGGAGTTCTT-1


title: 10X46_1_CTTCATGAGGCAAG-1


title: 10X46_1_CTTCTAGACCAACA-1


title: 10X46_1_CTTCACCTTGAAGA-1


title: 10X46_1_CTTATCGAGCGTAT-1


title: 10X46_1_CTTATCGATCTATC-1


title: 10X46_1_CTTACTGAGGACTT-1


title: 10X46_1_CTTATCGACACTCC-1


title: 10X46_1_CTTACAACTGCTCC-1


title: 10X46_1_CTTAGGGAAGTCTG-1


title: 10X46_1_CTTAAGCTTGTTCT-1


title: 10X46_1_CTTAAGCTTACTCT-1


title: 10X46_1_CTTAAGCTATCGTG-1


title: 10X46_1_CTTAAGCTCATGGT-1


title: 10X46_1_CTTCATGATTCTGT-1


title: 10X46_1_CTTGATTGGTGAGG-1


title: 10X46_1_CTTGTATGCACTGA-1


title: 10X46_1_CTTACTGAAGTTCG-1


title: 10X46_1_CTTAACACTTCGGA-1


title: 10X46_1_CTTAAAGATGCACA-1


title: 10X46_1_CTTATCGATAGTCG-1


title: 10X46_1_CTTAAAGAGCTTAG-1


title: 10X46_1_CTTAAAGACTTCGC-1


title: 10X46_1_CTTATCGAGTGTTG-1


title: 10X46_1_CTGTATACACTAGC-1


title: 10X46_1_CTGTGAGAATACCG-1


title: 10X46_1_CTGTGAGATGCATG-1


title: 10X46_1_CTGTAACTGGTCTA-1


title: 10X46_1_CTGTAACTGTAGCT-1


title: 10X46_1_CTGTGAGAGAGGGT-1


title: 10X46_1_CTGTAACTCTATTC-1


title: 10X46_1_CTGTAACTCACTAG-1


title: 10X46_1_CTTAGACTTAGCGT-1


title: 10X46_1_CTGGCACTCTTCGC-1


title: 10X46_1_CTGGCACTCTCAAG-1


title: 10X46_1_CTGGAAACGTGTCA-1


title: 10X46_1_CTGGAAACGTCTGA-1


title: 10X46_1_CTGGAAACGTTGCA-1


title: 10X46_1_CTGTAACTAACGAA-1


title: 10X46_1_CTGTAACTCTCGCT-1


title: 10X46_1_CTGCCAACCCGTTC-1


title: 10X46_1_CTGCGACTCATTCT-1


title: 10X46_1_CTGATGGACGTAGT-1


title: 10X46_1_CTGCAGCTGTACAC-1


title: 10X46_1_CTGATACTTGTTTC-1


title: 10X46_1_CTGAGAACGCATCA-1


title: 10X46_1_CTGAGCCTCTTCGC-1


title: 10X46_1_CTGAGCCTAAAGTG-1


title: 10X46_1_CTGAGAACCATCAG-1


title: 10X46_1_CTGGCACTAGGTCT-1


title: 10X46_1_CTGACCACTTGCTT-1


title: 10X46_1_CTGACCACGCGAAG-1


title: 10X46_1_CTGAAGTGTGTCGA-1


title: 10X46_1_CTGAATCTGTCAAC-1


title: 10X46_1_CTGACAGAAGTCTG-1


title: 10X46_1_CTGTAACTATCGTG-1


title: 10X46_1_CTGAATCTTCAAGC-1


title: 10X46_1_CTGAATCTTACAGC-1


title: 10X46_1_CTGAAGACACTGTG-1


title: 10X46_1_CTCTAATGTTACCT-1


title: 10X46_1_CTCGCATGTATCTC-1


title: 10X46_1_CTTAAAGACCAACA-1


title: 10X46_1_CTCGAGCTCTCTAT-1


title: 10X46_1_CTCGCATGCATCAG-1


title: 10X46_1_CTCGACTGTTTCGT-1


title: 10X46_1_CTCGACACTGCGTA-1


title: 10X46_1_CTGATACTGGTAAA-1


title: 10X46_1_CTCGACTGAGTGCT-1


title: 10X46_1_CTCGACACACCGAT-1


title: 10X46_1_CTCGAAGAGGAGGT-1


title: 10X46_1_CTCGAAGATTACCT-1


title: 10X46_1_CTCCGAACTTGGCA-1


title: 10X46_1_CTCATTGAGACGAG-1


title: 10X46_1_CTGAAGACGGATTC-1


title: 10X46_1_CTGAAGACGTTGTG-1


title: 10X46_1_CTCATTGAATCTCT-1


title: 10X46_1_CTCATTGACGCCTT-1


title: 10X46_1_CTCAGCTGTGAGGG-1


title: 10X46_1_CTCAGCTGACCTCC-1


title: 10X46_1_CTCCATCTCCTACC-1


title: 10X46_1_CTCATTGAGAGATA-1


title: 10X46_1_CTCAGAGAGAAAGT-1


title: 10X46_1_CTCAGAGATAGTCG-1


title: 10X46_1_CTCGAGCTAAGGCG-1


title: 10X46_1_CTCAGAGAACTCTT-1


title: 10X46_1_CTCAATTGTTTGGG-1


title: 10X46_1_CTCAATTGCCGAAT-1


title: 10X46_1_CTCAGCTGAGGGTG-1


title: 10X46_1_CTATTGTGGCATAC-1


title: 10X46_1_CTCAATTGACCAGT-1


title: 10X46_1_CTATTGACCGGGAA-1


title: 10X46_1_CTCGAAGAATTCCT-1


title: 10X46_1_CTCCGAACCCGAAT-1


title: 10X46_1_CTATGACTGTCTAG-1


title: 10X46_1_CTCCACGACTTGCC-1


title: 10X46_1_CTATGTTGATGCTG-1


title: 10X46_1_CTATGTTGGGCATT-1


title: 10X46_1_CTATGACTCACTCC-1


title: 10X46_1_CTATGTACTTGAGC-1


title: 10X46_1_CTCATTGAATCTTC-1


title: 10X46_1_CTATCCCTTAGCCA-1


title: 10X46_1_CTATCATGCGAGAG-1


title: 10X46_1_CTATCCCTGACTAC-1


title: 10X46_1_CTATCCCTGCTACA-1


title: 10X46_1_CTATCCCTCCATGA-1


title: 10X46_1_CTCAGCTGAGAACA-1


title: 10X46_1_CTATCAACACGACT-1


title: 10X46_1_CTATAGCTAACGAA-1