title: EB_Nkx2.5_Cre_mTmG91-G2


title: EB_Nkx2.5_Cre_mTmG91-F3

status: Public on Nov 10 2016

type: SRA

city: Pittsburgh

first: Guang

last: Li

state: PA

address: 530 45th St. 8116 Rangos Research Center

department: Developmental Biology

country: USA

institute: University of Pittsburgh

library_source: transcriptomic

data_processing:
Basecalls performed using RTA (Real Time Analysis. v1.18)
Sequenced reads were demultiplexed, and mapped to mouse genome GRCm38.79 with STAR 2.4.0
The mapped reads of each gene were quantified with HTSeq
Genome_build: GRCm38.79
Supplementary_files_format_and_content: tab-delimited text files include count number for each gene in each Sample

molecule: polyA RNA

organism: Mus musculus

source_name: embryoid body Nkx2.5Cre mTmG heart

extract_protocol:
Single cells derived from mouse embryonic hearts were captured, lysed, and pre-amplified in C1 microfluidic chips.
RNA libraries were prepared with Nextera XT reagent for illumina sequencing

tag: developmental stage

value: day 10

tag: tissue

value: embryoid body


title: EB_Nkx2.5_Cre_mTmG91-F7


title: EB_Nkx2.5_Cre_mTmG91-E8


title: EB_Nkx2.5_Cre_mTmG91-E9


title: EB_Nkx2.5_Cre_mTmG91-E6


title: EB_Nkx2.5_Cre_mTmG91-E2


title: EB_Nkx2.5_Cre_mTmG91-F2


title: EB_Nkx2.5_Cre_mTmG92-A6


title: EB_Nkx2.5_Cre_mTmG91-D9


title: EB_Nkx2.5_Cre_mTmG92-A12


title: EB_Nkx2.5_Cre_mTmG91-E11


title: EB_Nkx2.5_Cre_mTmG91-D7


title: EB_Nkx2.5_Cre_mTmG91-F1


title: EB_Nkx2.5_Cre_mTmG91-D4


title: EB_Nkx2.5_Cre_mTmG91-D6


title: EB_Nkx2.5_Cre_mTmG91-D11


title: EB_Nkx2.5_Cre_mTmG91-C9


title: EB_Nkx2.5_Cre_mTmG91-D2


title: EB_Nkx2.5_Cre_mTmG91-C7


title: EB_Nkx2.5_Cre_mTmG91-D1


title: EB_Nkx2.5_Cre_mTmG91-D12


title: EB_Nkx2.5_Cre_mTmG91-F8


title: EB_Nkx2.5_Cre_mTmG91-C6


title: EB_Nkx2.5_Cre_mTmG91-C4


title: EB_Nkx2.5_Cre_mTmG91-C5


title: EB_Nkx2.5_Cre_mTmG91-C12


title: EB_Nkx2.5_Cre_mTmG91-B8


title: EB_Nkx2.5_Cre_mTmG91-C10


title: EB_Nkx2.5_Cre_mTmG91-B7


title: EB_Nkx2.5_Cre_mTmG91-C1


title: EB_Nkx2.5_Cre_mTmG91-B3


title: EB_Nkx2.5_Cre_mTmG91-D5


title: EB_Nkx2.5_Cre_mTmG91-B12


title: EB_Nkx2.5_Cre_mTmG91-B10


title: EB_Nkx2.5_Cre_mTmG91-D8


title: EB_Nkx2.5_Cre_mTmG91-D3


title: EB_Nkx2.5_Cre_mTmG91-B5


title: EB_Nkx2.5_Cre_mTmG91-B9


title: EB_Nkx2.5_Cre_mTmG91-B11


title: EB_Nkx2.5_Cre_mTmG91-B1


title: EB_Nkx2.5_Cre_mTmG91-B4


title: EB_Nkx2.5_Cre_mTmG91-A9


title: EB_Nkx2.5_Cre_mTmG91-A5


title: EB_Nkx2.5_Cre_mTmG91-A8


title: e9.5_RA_99-H7

source_name: e9.5 right atrium

tag: strain

value: right atrium


title: EB_Nkx2.5_Cre_mTmG91-A10


title: EB_Nkx2.5_Cre_mTmG91-A11


title: EB_Nkx2.5_Cre_mTmG91-A2


title: EB_Nkx2.5_Cre_mTmG91-A3


title: e9.5_RA_99-H3


title: EB_Nkx2.5_Cre_mTmG91-B6


title: e9.5_RA_99-H5


title: EB_Nkx2.5_Cre_mTmG91-B2


title: e9.5_RA_99-H10


title: e9.5_RA_99-G9


title: e9.5_RA_99-H11


title: e9.5_RA_99-G7


title: e9.5_RA_99-G8


title: e9.5_RA_99-G12


title: e9.5_RA_99-F8


title: e9.5_RA_99-G11


title: e9.5_RA_99-F6


title: e9.5_RA_99-G10


title: e9.5_RA_99-G5


title: e9.5_RA_99-G6


title: e9.5_RA_99-G3


title: e9.5_RA_99-F5


title: e9.5_RA_99-F3


title: e9.5_RA_99-F4


title: EB_Nkx2.5_Cre_mTmG91-A4


title: EB_Nkx2.5_Cre_mTmG91-A7


title: e9.5_RA_99-F12


title: e9.5_RA_99-H2


title: e9.5_RA_99-F11


title: e9.5_RA_99-F10


title: e9.5_RA_99-E4


title: e9.5_RA_99-E8


title: e9.5_RA_99-E9


title: e9.5_RA_99-E1


title: e9.5_RA_99-E3


title: e9.5_RA_99-E10


title: e9.5_RA_99-E7


title: e9.5_RA_99-E11


title: e9.5_RA_99-D9


title: e9.5_RA_99-D6


title: e9.5_RA_99-D4


title: e9.5_RA_99-D2


title: e9.5_RA_99-C7


title: e9.5_RA_99-D3


title: e9.5_RA_99-D11


title: e9.5_RA_99-C4


title: e9.5_RA_99-C5


title: e9.5_RA_99-D1


title: e9.5_RA_99-D12


title: e9.5_RA_99-D10


title: e9.5_RA_99-C6


title: e9.5_RA_99-E6


title: e9.5_RA_99-C3


title: e9.5_RA_99-E2


title: e9.5_RA_99-C10


title: e9.5_RA_99-C12


title: e9.5_RA_99-C2


title: e9.5_RA_99-C11


title: e9.5_RA_99-B9


title: e9.5_RA_99-C1


title: e9.5_RA_99-B8


title: e9.5_RA_99-B11


title: e9.5_RA_99-B2


title: e9.5_RA_99-B3


title: e9.5_RA_99-B1


title: e9.5_RA_99-A7


title: e9.5_RA_99-A5


title: e9.5_RA_99-A9


title: e9.5_RA_99-A12


title: e9.5_RA_99-A11


title: e9.5_RA_99-A4


title: e9.5_RA_99-A1


title: e9.5_LV_98-G11

source_name: e9.5 left ventricle

value: left ventricle


title: e9.5_LV_98-G7


title: e9.5_LV_98-H1


title: e9.5_LV_98-G1


title: e9.5_LV_98-G2


title: e9.5_LV_98-H3


title: e9.5_LV_98-F6


title: e9.5_LV_98-G9


title: e9.5_LV_98-E7


title: e9.5_LV_98-F1


title: e9.5_LV_98-F5


title: e9.5_LV_98-E5


title: e9.5_LV_98-F11


title: e9.5_LV_98-E12


title: e9.5_LV_98-D8


title: e9.5_LV_98-D7


title: e9.5_LV_98-D6


title: e9.5_LV_98-D12


title: e9.5_LV_98-D4


title: e9.5_LV_98-D2


title: e9.5_LV_98-D10


title: e9.5_LV_98-D3


title: e9.5_LV_98-C9


title: e9.5_LV_98-F2


title: e9.5_LV_98-C8


title: e9.5_LV_98-F9


title: e9.5_LV_98-C5


title: e9.5_LV_98-C7


title: e9.5_LV_98-B8


title: e9.5_LV_98-C6


title: e9.5_LV_98-B6


title: e9.5_LV_98-B2


title: e9.5_LV_98-B12


title: e9.5_LV_98-C4


title: e9.5_LV_98-E11


title: e9.5_LV_98-C11


title: e9.5_LV_98-B5


title: e9.5_LV_98-B3


title: e9.5_LV_98-A9


title: e9.5_RS_97-H7

source_name: e9.5 right ventricular septum

value: right ventricular septum


title: e9.5_LV_98-D1


title: e9.5_LV_98-C12


title: e9.5_LV_98-B1


title: e9.5_LV_98-C2


title: e9.5_LV_98-C10


title: e9.5_RS_97-H5


title: e9.5_RS_97-G10


title: e9.5_RS_97-G12


title: e9.5_RS_97-G1


title: e9.5_RS_97-H4


title: e9.5_RS_97-G11


title: e9.5_RS_97-H11


title: e9.5_RS_97-H10


title: e9.5_RS_97-G7


title: e9.5_LV_98-B10


title: e9.5_RS_97-F6


title: e9.5_RS_97-F7


title: e9.5_RS_97-G3


title: e9.5_RS_97-F5


title: e9.5_RS_97-G5


title: e9.5_RS_97-F4


title: e9.5_LV_98-B7


title: e9.5_RS_97-F3


title: e9.5_RS_97-F12


title: e9.5_RS_97-F2


title: e9.5_RS_97-F11


title: e9.5_RS_97-G4


title: e9.5_RS_97-E6


title: e9.5_RS_97-F10


title: e9.5_RS_97-E12


title: e9.5_RS_97-E3


title: e9.5_RS_97-E4


title: e9.5_RS_97-E11


title: e9.5_RS_97-D5


title: e9.5_RS_97-D6


title: e9.5_RS_97-D12


title: e9.5_RS_97-C7


title: e9.5_RS_97-D10


title: e9.5_RS_97-C6


title: e9.5_RS_97-C12


title: e9.5_RS_97-C4


title: e9.5_RS_97-C10


title: e9.5_RS_97-C1


title: e9.5_RS_97-B7


title: e9.5_RS_97-B6


title: e9.5_RS_97-B5


title: e9.5_RS_97-E5


title: e9.5_RS_97-B4


title: e9.5_RS_97-E2


title: e9.5_RS_97-B11


title: e9.5_RS_97-B2


title: e9.5_RS_97-A7


title: e9.5_RS_97-B10


title: e9.5_RS_97-A11


title: e9.5_RS_97-A1


title: e9.5_RS_97-A5


title: e9.5_RS_97-A3


title: e9.5_RV_96-H7

source_name: e9.5 right ventricle

value: right ventricle


title: e9.5_RV_96-H6


title: e9.5_RS_97-A2


title: e9.5_RV_96-H9


title: e9.5_RV_96-H8


title: e9.5_RS_97-E10


title: e9.5_RS_97-A12


title: e9.5_RS_97-C11


title: e9.5_RV_96-H10


title: e9.5_RS_97-D3


title: e9.5_RV_96-H4


title: e9.5_RV_96-G2


title: e9.5_RS_97-C5


title: e9.5_RV_96-F9


title: e9.5_RV_96-G1


title: e9.5_RV_96-F8


title: e9.5_RV_96-F6


title: e9.5_RV_96-H5


title: e9.5_RV_96-H1


title: e9.5_RV_96-G10


title: e9.5_RV_96-F2


title: e9.5_RV_96-F7


title: e9.5_RV_96-F5


title: e9.5_RV_96-F12


title: e9.5_RV_96-F11


title: e9.5_RV_96-F3


title: e9.5_RV_96-E8


title: e9.5_RV_96-E3


title: e9.5_RV_96-E5


title: e9.5_RV_96-E4


title: e9.5_RV_96-E12


title: e9.5_RV_96-E7


title: e9.5_RV_96-E6


title: e9.5_RV_96-E10


title: e9.5_RV_96-E2


title: e9.5_RV_96-G3


title: e9.5_RV_96-E11


title: e9.5_RV_96-D6


title: e9.5_RV_96-D5


title: e9.5_RV_96-C11


title: e9.5_RV_96-D12


title: e9.5_RV_96-E9


title: e9.5_RV_96-B5


title: e9.5_RV_96-C9


title: e9.5_RV_96-B4


title: e9.5_RV_96-C6


title: e9.5_RV_96-F4


title: e9.5_RV_96-C5


title: e9.5_RV_96-A8


title: e9.5_RV_96-B1


title: e9.5_RV_96-D10


title: e9.5_RV_96-C1


title: e9.5_RV_96-A5


title: e9.5_RV_96-B2


title: e9.5_RV_96-C4


title: e9.5_RV_96-B12


title: e9.5_RV_96-B6


title: e9.5_RV_96-B9


title: e9.5_RV_96-A9


title: e9.5_RV_96-C12


title: e9.5_RV_96-A10


title: e9.5_RV_96-A6


title: e9.5_RV_96-A7


title: e9.5_RV_96-A4


title: e9.5_RV_96-A2


title: e9.5_RV_96-A12


title: e9.5_RV_96-A11


title: e9.5_LA_95-G8

source_name: e9.5 left atrium

value: left atrium


title: e9.5_LA_95-G3


title: e9.5_RV_96-B8


title: e9.5_LA_95-H8


title: e9.5_LA_95-G9


title: e9.5_RV_96-B10


title: e9.5_LA_95-F8


title: e9.5_LA_95-G1


title: e9.5_LA_95-H1


title: e9.5_LA_95-H3


title: e9.5_LA_95-H12


title: e9.5_LA_95-G12


title: e9.5_LA_95-G5


title: e9.5_LA_95-F7


title: e9.5_LA_95-G11


title: e9.5_LA_95-F6


title: e9.5_LA_95-E7


title: e9.5_LA_95-F12


title: e9.5_LA_95-F9


title: e9.5_LA_95-E5


title: e9.5_LA_95-E12


title: e9.5_LA_95-E3


title: e9.5_LA_95-F3


title: e9.5_LA_95-F5


title: e9.5_LA_95-F2


title: e9.5_LA_95-F1


title: e9.5_LA_95-E11


title: e9.5_LA_95-E1


title: e9.5_LA_95-D8


title: e9.5_LA_95-D9


title: e9.5_LA_95-D7


title: e9.5_LA_95-D6


title: e9.5_LA_95-D4


title: e9.5_LA_95-D5


title: e9.5_LA_95-C8


title: e9.5_LA_95-C6


title: e9.5_LA_95-D12


title: e9.5_LA_95-C4


title: e9.5_LA_95-C5


title: e9.5_LA_95-C9


title: e9.5_LA_95-C12


title: e9.5_LA_95-C2


title: e9.5_LA_95-D3


title: e9.5_LA_95-C3


title: e9.5_LA_95-B8


title: e9.5_LA_95-B4


title: e9.5_LA_95-C1


title: e9.5_LA_95-C11


title: e9.5_LA_95-B3


title: e9.5_LA_95-B7


title: e9.5_LA_95-B11


title: e9.5_LA_95-A9


title: e9.5_LA_95-D10


title: e9.5_LA_95-A7


title: e9.5_LA_95-A8


title: e9.5_LA_95-A3


title: e9.5_LA_95-A12


title: e9.5_LA_95-A10


title: e9.5_LA_95-A1


title: e9.5_LS_94-H5

source_name: e9.5 left ventricular septum

value: left ventricular septum


title: e9.5_LS_94-H4


title: e9.5_LS_94-H6


title: e9.5_LA_95-C10


title: e9.5_LS_94-H9


title: e9.5_LS_94-H3


title: e9.5_LS_94-G5


title: e9.5_LS_94-H1


title: e9.5_LS_94-G4


title: e9.5_LA_95-B5


title: e9.5_LS_94-G12


title: e9.5_LS_94-G11


title: e9.5_LS_94-G1


title: e9.5_LA_95-B10


title: e9.5_LA_95-B1


title: e9.5_LS_94-F7


title: e9.5_LA_95-A2


title: e9.5_LS_94-F6


title: e9.5_LS_94-F5


title: e9.5_LS_94-F3


title: e9.5_LS_94-F2


title: e9.5_LS_94-F11


title: e9.5_LS_94-H2


title: e9.5_LS_94-H12


title: e9.5_LS_94-E9


title: e9.5_LS_94-F10


title: e9.5_LS_94-E8


title: e9.5_LS_94-F1


title: e9.5_LS_94-E7


title: e9.5_LS_94-E3


title: e9.5_LS_94-E10


title: e9.5_LS_94-E12


title: e9.5_LS_94-D9


title: e9.5_LS_94-G3


title: e9.5_LS_94-F8


title: e9.5_LS_94-D8


title: e9.5_LS_94-D6


title: e9.5_LS_94-D12


title: e9.5_LS_94-D2


title: e9.5_LS_94-D3


title: e9.5_LS_94-D4


title: e9.5_LS_94-D11


title: e9.5_LS_94-D1


title: e9.5_LS_94-H11


title: e9.5_LS_94-D10


title: e9.5_LS_94-C9


title: e9.5_LS_94-E1


title: e9.5_LS_94-C3


title: e9.5_LS_94-C7


title: e9.5_LS_94-E6


title: e9.5_LS_94-C6


title: e9.5_LS_94-C11


title: e9.5_LS_94-C10


title: e9.5_LS_94-B5


title: e9.5_LS_94-B4


title: e9.5_LS_94-C2


title: e9.5_LS_94-B6


title: e9.5_LS_94-B11


title: e9.5_LS_94-B10


title: e9.5_LS_94-B12


title: e9.5_LS_94-C1


title: e9.5_LS_94-A5


title: e9.5_LS_94-A7


title: e9.5_LS_94-D5


title: e9.5_LS_94-A9


title: e9.5_LS_94-A6


title: e9.5_LS_94-A3


title: e9.5_LS_94-A4


title: e9.5_LS_94-A11


title: e9.5_OT_93-H9

source_name: e9.5 outflow tract

value: outflow tract


title: e9.5_LS_94-A1


title: e9.5_OT_93-H7


title: e9.5_OT_93-H6


title: e9.5_OT_93-H5


title: e9.5_OT_93-H8


title: e9.5_OT_93-H3


title: e9.5_OT_93-H12


title: e9.5_OT_93-H1


title: e9.5_OT_93-G4


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title: e9.5_OT_93-G3


title: e9.5_OT_93-G12


title: e9.5_OT_93-G1


title: e9.5_OT_93-F9


title: e9.5_OT_93-G7


title: e9.5_OT_93-G11


title: e9.5_OT_93-G2


title: e9.5_OT_93-G6


title: e9.5_OT_93-G5


title: e9.5_OT_93-G10


title: e9.5_OT_93-F8


title: e9.5_OT_93-F6


title: e9.5_OT_93-F7


title: e9.5_OT_93-F4


title: e9.5_OT_93-F1


title: e9.5_LS_94-A10


title: e9.5_OT_93-F2


title: e9.5_OT_93-F5


title: e9.5_OT_93-E5


title: e9.5_OT_93-F3


title: e9.5_OT_93-H4


title: e9.5_OT_93-E9


title: e9.5_OT_93-F10


title: e9.5_OT_93-E6


title: e9.5_OT_93-E8


title: e9.5_OT_93-E10


title: e9.5_OT_93-D9


title: e9.5_OT_93-D7


title: e9.5_OT_93-E4


title: e9.5_OT_93-D3


title: e9.5_OT_93-D2


title: e9.5_OT_93-D1


title: e9.5_OT_93-D12


title: e9.5_OT_93-D6


title: e9.5_OT_93-D8


title: e9.5_OT_93-E1


title: e9.5_OT_93-D4


title: e9.5_OT_93-E2


title: e9.5_OT_93-C4


title: e9.5_OT_93-E7


title: e9.5_OT_93-E12


title: e9.5_OT_93-D10


title: e9.5_OT_93-D11


title: e9.5_OT_93-B9


title: e9.5_OT_93-B5


title: e9.5_OT_93-C2


title: e9.5_OT_93-C1


title: e9.5_OT_93-C12


title: e9.5_OT_93-C11


title: e9.5_OT_93-C10


title: e9.5_OT_93-B8


title: e9.5_OT_93-B7


title: e9.5_OT_93-A9


title: e9.5_OT_93-B3


title: e9.5_OT_93-A2


title: e9.5_OT_93-A5


title: e9.5_OT_93-A1


title: e9.5_OT_93-A4


title: e9.5_OT_93-D5


title: e9.5_OT_93-A10


title: e9.5_Nkx2.5wt_83-H5

source_name: e9.5 Nkx2.5 WT heart

value: heart


title: e9.5_OT_93-B1


title: e9.5_OT_93-A3


title: e9.5_OT_93-A6


title: e9.5_OT_93-C9


title: e9.5_OT_93-A8


title: e9.5_OT_93-B2


title: e9.5_OT_93-C5


title: e9.5_OT_93-A12


title: e9.5_Nkx2.5wt_83-H4


title: e9.5_Nkx2.5wt_83-H9


title: e9.5_Nkx2.5wt_83-H7


title: e9.5_OT_93-A7


title: e9.5_Nkx2.5wt_83-H10


title: e9.5_Nkx2.5wt_83-H11


title: e9.5_Nkx2.5wt_83-H2


title: e9.5_Nkx2.5wt_83-G9


title: e9.5_Nkx2.5wt_83-G7


title: e9.5_Nkx2.5wt_83-G6


title: e9.5_Nkx2.5wt_83-G5


title: e9.5_Nkx2.5wt_83-G3


title: e9.5_Nkx2.5wt_83-G4


title: e9.5_Nkx2.5wt_83-G2


title: e9.5_Nkx2.5wt_83-H6


title: e9.5_Nkx2.5wt_83-G12


title: e9.5_Nkx2.5wt_83-G11


title: e9.5_Nkx2.5wt_83-G10


title: e9.5_Nkx2.5wt_83-F9


title: e9.5_Nkx2.5wt_83-F8


title: e9.5_Nkx2.5wt_83-F7


title: e9.5_Nkx2.5wt_83-G1


title: e9.5_Nkx2.5wt_83-F6


title: e9.5_Nkx2.5wt_83-F5


title: e9.5_Nkx2.5wt_83-F4


title: e9.5_Nkx2.5wt_83-F3


title: e9.5_Nkx2.5wt_83-F2


title: e9.5_Nkx2.5wt_83-F11


title: e9.5_Nkx2.5wt_83-F10


title: e9.5_Nkx2.5wt_83-H12


title: e9.5_Nkx2.5wt_83-F1


title: e9.5_Nkx2.5wt_83-E7


title: e9.5_Nkx2.5wt_83-E5


title: e9.5_Nkx2.5wt_83-E4


title: e9.5_Nkx2.5wt_83-E3


title: e9.5_Nkx2.5wt_83-E12


title: e9.5_Nkx2.5wt_83-E1


title: e9.5_Nkx2.5wt_83-D8


title: e9.5_Nkx2.5wt_83-D7


title: e9.5_Nkx2.5wt_83-D6


title: e9.5_Nkx2.5wt_83-D5


title: e9.5_Nkx2.5wt_83-D4


title: e9.5_Nkx2.5wt_83-D3


title: e9.5_Nkx2.5wt_83-D2


title: e9.5_Nkx2.5wt_83-D1


title: e9.5_Nkx2.5wt_83-C2


title: e9.5_Nkx2.5wt_83-C5


title: e9.5_Nkx2.5wt_83-C8


title: e9.5_Nkx2.5wt_83-F12


title: e9.5_Nkx2.5wt_83-C6


title: e9.5_Nkx2.5wt_83-C11


title: e9.5_Nkx2.5wt_83-C10


title: e9.5_Nkx2.5wt_83-C1


title: e9.5_Nkx2.5wt_83-E8


title: e9.5_Nkx2.5wt_83-E2


title: e9.5_Nkx2.5wt_83-C12


title: e9.5_Nkx2.5wt_83-B7


title: e9.5_Nkx2.5wt_83-B9


title: e9.5_Nkx2.5wt_83-B8


title: e9.5_Nkx2.5wt_83-E11


title: e9.5_Nkx2.5wt_83-E10


title: e9.5_Nkx2.5wt_83-D9


title: e9.5_Nkx2.5wt_83-B6


title: e9.5_Nkx2.5wt_83-B4


title: e9.5_Nkx2.5wt_83-B5


title: e9.5_Nkx2.5wt_83-B3


title: e9.5_Nkx2.5wt_83-B1


title: e9.5_Nkx2.5wt_83-B11


title: e9.5_Nkx2.5wt_83-B12


title: e9.5_Nkx2.5wt_83-A8


title: e9.5_Nkx2.5wt_83-A9


title: e9.5_Nkx2.5wt_83-D12


title: e9.5_Nkx2.5wt_83-A5


title: e9.5_Nkx2.5wt_83-A3


title: e9.5_Nkx2.5wt_83-A2


title: e9.5_Nkx2.5wt_83-A11


title: e9.5_Nkx2.5wt_83-A10


title: e9.5_Nkx2.5wt_82-H9


title: e9.5_Nkx2.5wt_83-A4


title: e9.5_Nkx2.5wt_82-H3


title: e9.5_Nkx2.5wt_82-H6


title: e9.5_Nkx2.5wt_82-H1


title: e9.5_Nkx2.5wt_83-A1


title: e9.5_Nkx2.5wt_82-H7


title: e9.5_Nkx2.5wt_82-H8


title: e9.5_Nkx2.5wt_82-G6


title: e9.5_Nkx2.5wt_82-H4


title: e9.5_Nkx2.5wt_82-H12


title: e9.5_Nkx2.5wt_82-G7


title: e9.5_Nkx2.5wt_82-H10


title: e9.5_Nkx2.5wt_82-H11


title: e9.5_Nkx2.5wt_82-G3


title: e9.5_Nkx2.5wt_82-G8


title: e9.5_Nkx2.5wt_82-G12


title: e9.5_Nkx2.5wt_82-G4


title: e9.5_Nkx2.5wt_82-G5


title: e9.5_Nkx2.5wt_83-A6


title: e9.5_Nkx2.5wt_82-G11


title: e9.5_Nkx2.5wt_83-A12


title: e9.5_Nkx2.5wt_82-G1


title: e9.5_Nkx2.5wt_82-G10


title: e9.5_Nkx2.5wt_82-H5


title: e9.5_Nkx2.5wt_82-F6


title: e9.5_Nkx2.5wt_82-F5


title: e9.5_Nkx2.5wt_82-F4


title: e9.5_Nkx2.5wt_82-F9


title: e9.5_Nkx2.5wt_82-F3


title: e9.5_Nkx2.5wt_82-F12


title: e9.5_Nkx2.5wt_82-F1


title: e9.5_Nkx2.5wt_82-F11


title: e9.5_Nkx2.5wt_82-E8


title: e9.5_Nkx2.5wt_82-E7


title: e9.5_Nkx2.5wt_82-E6


title: e9.5_Nkx2.5wt_82-E4


title: e9.5_Nkx2.5wt_82-E5


title: e9.5_Nkx2.5wt_82-E3


title: e9.5_Nkx2.5wt_82-E11


title: e9.5_Nkx2.5wt_82-E12


title: e9.5_Nkx2.5wt_82-D8


title: e9.5_Nkx2.5wt_82-D5


title: e9.5_Nkx2.5wt_82-D7


title: e9.5_Nkx2.5wt_82-D4


title: e9.5_Nkx2.5wt_82-D6


title: e9.5_Nkx2.5wt_82-F8


title: e9.5_Nkx2.5wt_82-D12


title: e9.5_Nkx2.5wt_82-F7


title: e9.5_Nkx2.5wt_82-D2


title: e9.5_Nkx2.5wt_82-D3


title: e9.5_Nkx2.5wt_82-D11


title: e9.5_Nkx2.5wt_82-D10


title: e9.5_Nkx2.5wt_82-D1


title: e9.5_Nkx2.5wt_82-C9


title: e9.5_Nkx2.5wt_82-C8


title: e9.5_Nkx2.5wt_82-C6


title: e9.5_Nkx2.5wt_82-E9


title: e9.5_Nkx2.5wt_82-C5


title: e9.5_Nkx2.5wt_82-C7


title: e9.5_Nkx2.5wt_82-C4


title: e9.5_Nkx2.5wt_82-C3


title: e9.5_Nkx2.5wt_82-C11


title: e9.5_Nkx2.5wt_82-C12


title: e9.5_Nkx2.5wt_82-C10


title: e9.5_Nkx2.5wt_82-B9


title: e9.5_Nkx2.5wt_82-B7


title: e9.5_Nkx2.5wt_82-B8


title: e9.5_Nkx2.5wt_82-B6


title: e9.5_Nkx2.5wt_82-B5


title: e9.5_Nkx2.5wt_82-B10


title: e9.5_Nkx2.5wt_82-B4


title: e9.5_Nkx2.5wt_82-B3


title: e9.5_Nkx2.5wt_82-B11


title: e9.5_Nkx2.5wt_82-A8


title: e9.5_Nkx2.5wt_82-A7


title: e9.5_Nkx2.5wt_82-B1


title: e9.5_Nkx2.5wt_82-A9


title: e9.5_Nkx2.5wt_82-A6


title: e9.5_Nkx2.5wt_82-A4


title: e9.5_Nkx2.5wt_82-A5


title: e9.5_Nkx2.5mut_87-G3

source_name: e9.5 Nkx2.5 mut heart


title: e9.5_Nkx2.5mut_87-G5


title: e9.5_Nkx2.5wt_82-A2


title: e9.5_Nkx2.5wt_82-A12


title: e9.5_Nkx2.5mut_87-H3


title: e9.5_Nkx2.5wt_82-A1


title: e9.5_Nkx2.5mut_87-G1


title: e9.5_Nkx2.5mut_87-H1


title: e9.5_Nkx2.5mut_87-F9


title: e9.5_Nkx2.5mut_87-E9


title: e9.5_Nkx2.5wt_82-B12


title: e9.5_Nkx2.5mut_87-F8


title: e9.5_Nkx2.5mut_87-H2


title: e9.5_Nkx2.5mut_87-F2


title: e9.5_Nkx2.5mut_87-F3


title: e9.5_Nkx2.5mut_87-E11


title: e9.5_Nkx2.5mut_87-E3


title: e9.5_Nkx2.5mut_87-F6


title: e9.5_Nkx2.5mut_87-E8


title: e9.5_Nkx2.5mut_87-E7


title: e9.5_Nkx2.5mut_87-E4


title: e9.5_Nkx2.5mut_87-E5


title: e9.5_Nkx2.5mut_87-E2


title: e9.5_Nkx2.5mut_87-D7


title: e9.5_Nkx2.5mut_87-G2


title: e9.5_Nkx2.5mut_87-D6


title: e9.5_Nkx2.5mut_87-D12


title: e9.5_Nkx2.5mut_87-F5


title: e9.5_Nkx2.5mut_87-D2


title: e9.5_Nkx2.5mut_87-D4


title: e9.5_Nkx2.5mut_87-F1


title: e9.5_Nkx2.5mut_87-D10


title: e9.5_Nkx2.5mut_87-C8


title: e9.5_Nkx2.5mut_87-C9


title: e9.5_Nkx2.5mut_87-C3


title: e9.5_Nkx2.5mut_87-C4


title: e9.5_Nkx2.5mut_87-C5


title: e9.5_Nkx2.5mut_87-C2


title: e9.5_Nkx2.5mut_87-E12


title: e9.5_Nkx2.5mut_87-C7


title: e9.5_Nkx2.5mut_87-C10


title: e9.5_Nkx2.5mut_87-B8


title: e9.5_Nkx2.5mut_87-B7


title: e9.5_Nkx2.5mut_87-B9


title: e9.5_Nkx2.5mut_87-B3


title: e9.5_Nkx2.5mut_87-E1


title: e9.5_Nkx2.5mut_87-B2


title: e9.5_Nkx2.5mut_87-B4


title: e9.5_Nkx2.5mut_87-B12


title: e9.5_Nkx2.5mut_87-D5


title: e9.5_Nkx2.5mut_87-B11


title: e9.5_Nkx2.5mut_87-B10


title: e9.5_Nkx2.5mut_87-B1


title: e9.5_Nkx2.5mut_87-A8


title: e9.5_Nkx2.5mut_87-A9


title: e9.5_Nkx2.5mut_87-A10


title: e9.5_Nkx2.5mut_87-A12


title: e9.5_Nkx2.5mut_87-C11


title: e9.5_Nkx2.5mut_87-A4


title: e9.5_Nkx2.5mut_87-C1


title: e9.5_Nkx2.5mut_86-H7


title: e9.5_Nkx2.5mut_86-H4


title: e9.5_Nkx2.5mut_86-H5


title: e9.5_Nkx2.5mut_86-H3


title: e9.5_Nkx2.5mut_86-H6


title: e9.5_Nkx2.5mut_86-G9


title: e9.5_Nkx2.5mut_86-H1


title: e9.5_Nkx2.5mut_86-H2


title: e9.5_Nkx2.5mut_87-B6


title: e9.5_Nkx2.5mut_86-G2


title: e9.5_Nkx2.5mut_86-G8


title: e9.5_Nkx2.5mut_86-G10


title: e9.5_Nkx2.5mut_86-F9


title: e9.5_Nkx2.5mut_86-G1


title: e9.5_Nkx2.5mut_86-F7


title: e9.5_Nkx2.5mut_86-F5


title: e9.5_Nkx2.5mut_86-G12


title: e9.5_Nkx2.5mut_86-G7


title: e9.5_Nkx2.5mut_86-F4


title: e9.5_Nkx2.5mut_86-F2


title: e9.5_Nkx2.5mut_86-G6


title: e9.5_Nkx2.5mut_86-F8


title: e9.5_Nkx2.5mut_86-E9


title: e9.5_Nkx2.5mut_86-G4


title: e9.5_Nkx2.5mut_86-F12


title: e9.5_Nkx2.5mut_86-F10


title: e9.5_Nkx2.5mut_86-F1


title: e9.5_Nkx2.5mut_87-A1


title: e9.5_Nkx2.5mut_86-E5


title: e9.5_Nkx2.5mut_86-E4


title: e9.5_Nkx2.5mut_86-D8


title: e9.5_Nkx2.5mut_86-E12


title: e9.5_Nkx2.5mut_86-E2


title: e9.5_Nkx2.5mut_86-E3


title: e9.5_Nkx2.5mut_86-E11


title: e9.5_Nkx2.5mut_86-E10


title: e9.5_Nkx2.5mut_86-D7


title: e9.5_Nkx2.5mut_86-D6


title: e9.5_Nkx2.5mut_86-D4


title: e9.5_Nkx2.5mut_86-D12


title: e9.5_Nkx2.5mut_86-D3


title: e9.5_Nkx2.5mut_86-D2


title: e9.5_Nkx2.5mut_86-D11


title: e9.5_Nkx2.5mut_86-D10


title: e9.5_Nkx2.5mut_86-D1


title: e9.5_Nkx2.5mut_86-C7


title: e9.5_Nkx2.5mut_86-C9


title: e9.5_Nkx2.5mut_86-C6


title: e9.5_Nkx2.5mut_86-C2


title: e9.5_Nkx2.5mut_86-C4


title: e9.5_Nkx2.5mut_86-C12


title: e9.5_Nkx2.5mut_86-C11


title: e9.5_Nkx2.5mut_86-C10


title: e9.5_Nkx2.5mut_86-B9


title: e9.5_Nkx2.5mut_86-B7


title: e9.5_Nkx2.5mut_86-B6


title: e9.5_Nkx2.5mut_86-E7


title: e9.5_Nkx2.5mut_86-B5


title: e9.5_Nkx2.5mut_86-B4


title: e9.5_Nkx2.5mut_86-B12


title: e9.5_Nkx2.5mut_86-B11


title: e9.5_Nkx2.5mut_86-A7


title: e9.5_Nkx2.5mut_86-A6


title: e9.5_Nkx2.5mut_86-D5


title: e9.5_Nkx2.5mut_86-B2


title: e9.5_Nkx2.5mut_86-A5


title: e9.5_Nkx2.5mut_86-B1


title: e9.5_Nkx2.5mut_86-A8


title: BB30105_GEMM_BMDM

city: Philadelphia

first: Robert

middle: L

last: Bowman

address: 421 Curie Blvd

department: Cancer Biology

institute: University of Pennsylvania School of Medicine

data_processing:
Basecalls performed using CASAVA version 1.8
Reads were mapped to the mouse genome mm10 using the STAR aligner v2.5.0e with default parameters.
Reads were mapped to genes using the default parameters provided b STAR (based on HT-Seq counts).
Gene counts were normalized and analyzed for differential exprssion with DESeq2 in R.
Genome_build: mm10
Supplementary_files_format_and_content: txt file of raw counts from STAR

source_name: tumor associated macrophage

growth_protocol: For the glioma generation intracranial injections were performed on 5-6 week old mice as previously described (Pyonteck et al., 2013).  Briefly, mice were fully anesthetized with ketamine/xylazine and bupivacaine was applied as a local anesthetic.  Using a stereotactic apparatus, cells were injected into the right frontal cortex (1 mm caudal, 1.5 mm lateral from bregma, 2-3 mm deep).   3x10^5 DF1 cells (1:1 mixture of DF1: RCAS-PDGFB-HA, and DF1:RCAS:shP53)  were injected at week 6 of age into Flt3:Cre Rosa26:mTmG mice.

extract_protocol:
RNA was isolated from TriZOL with a chloroform extraction, following by isopropanol precipitation with glycogen carrier.
Libraries were generated using the Smart-Seq kit (Clonetech).

treatment_protocol: Tumor bearing mice were anesthetized with 1.25% Avertin, and transcardially perfused with PBS. Tumors were macrodissected and dissociated using the Mouse Tumor Dissociation Kit (mTDK, Miltenyi) using the OctoMACS disocciator.  Tumors were filtered through a 40 μM mesh filter and underwent RBC lysis (PharmLyse BD).  Tissue underwent myelin removal using the Myelin Removal Beads (Miltenyi).  Single cell suspensions were FC blocked (BD cat #553141) for 15 minutes at 4 degrees Celsius.  Cells were then incubated with directly conjugated antibody panels for 15 minutes at 4 degrees Celsius.  Cell suspensions were washed (PBS +2% fetal bovine serum) and resuspended in a DAPI solution.  All flow cytometry analysis was completed on a BD Fortessa device at the Flow Cytometry Core Facility (FCCF) at MSKCC.  All cell sorting was completed on an Aria III device at the FCCF at MSKCC. Tumor assocaited microglia (MG) were isoalted as Cd45+Cd11b+Ly6c-Ly6g-GFP-TdTomato+ cells while tumor associated bone marrow derived macrophages (BMDM) were isolated  as  Cd45+Cd11b+Ly6c-Ly6g-GFP+TdTomato- cells.  Samples were sorted directly into TriZOL LS.

tag: cell type

value: TAM Bone marrow derived macrophage

value: GEMM


title: e9.5_Nkx2.5mut_86-A3


title: 68702_GEMM_MG

source_name: tumor associated microglia

value: TAM Microglia


title: BB30105_GEMM_MG


title: e9.5_Nkx2.5mut_86-A9


title: e9.5_Nkx2.5mut_86-A11


title: e8.5_V_V-H7

data_processing:
Basecalls performed using RTA (Real Time Analysis. v1.18)
Sequenced reads were demutiplexed, and mapped to mouse genome GRCm38.79 with STAR 2.4.0
The mapped reads of each gene were counted with HTSeq, and TPM were calculated with R script.
Genome_build: GRCm38.79
Supplementary_files_format_and_content: tab-delimited text files include counts number for each gene in each Sample

source_name: e8.5 ventricle

extract_protocol:
Mouse embryonic hearts derived single cells were captured, lysed, and pre-amplified in C1 microfluidic chips.
RNA libraries were prepared with Nextera XT reagent for illumina sequencing

value: ventricle


title: e8.5_V_V-G7


title: e8.5_V_V-G6


title: e8.5_V_V-G5


title: e8.5_V_V-G8


title: e8.5_V_V-H11


title: BB30101_GEMM_BMDM


title: BB30101_GEMM_MG


title: 68702_GEMM_BMDM


title: e8.5_V_V-G2


title: e8.5_V_V-H6


title: e8.5_V_V-H5


title: e8.5_V_V-G10


title: e8.5_V_V-F7


title: e8.5_V_V-G11


title: e8.5_V_V-F9


title: e8.5_V_V-H12


title: e8.5_V_V-F8


title: e8.5_V_V-H1


title: e8.5_V_V-F3


title: e8.5_V_V-F4


title: e8.5_V_V-F12


title: e8.5_V_V-F11


title: e8.5_V_V-E8


title: e8.5_V_V-F1


title: e8.5_V_V-E5


title: e8.5_V_V-E4


title: e8.5_V_V-G9


title: e8.5_V_V-E3


title: e8.5_V_V-E12


title: e8.5_V_V-E11


title: e8.5_V_V-D9


title: e8.5_V_V-E6


title: e8.5_V_V-E2


title: e8.5_V_V-H10


title: e8.5_V_V-D8


title: e8.5_V_V-F5


title: e8.5_V_V-D7


title: e8.5_V_V-D4


title: e8.5_V_V-D2


title: e8.5_V_V-D5


title: e8.5_V_V-D11


title: e8.5_V_V-C6


title: e8.5_V_V-E7


title: e8.5_V_V-C9


title: e8.5_V_V-F2


title: e8.5_V_V-D1


title: e8.5_V_V-D12


title: e8.5_V_V-C7


title: e8.5_V_V-C2


title: e8.5_V_V-C12


title: e8.5_V_V-C5


title: e8.5_V_V-C11


title: e8.5_V_V-C4


title: e8.5_V_V-C10


title: e8.5_V_V-C1


title: e8.5_V_V-B8


title: e8.5_V_V-B6


title: e8.5_V_V-B5


title: e8.5_V_V-B2


title: e8.5_V_V-B3


title: e8.5_V_V-B9


title: e8.5_V_V-B12


title: e8.5_V_V-B11


title: e8.5_V_V-B1


title: e8.5_V_V-A9


title: e8.5_V_V-A3


title: nkm-H6


title: nkm-H5


title: nkm-H4


title: e8.5_V_V-A10


title: nkm-H7


title: nkm-H3


title: nkm-H1


title: nkm-H2


title: e8.5_V_V-B7


title: nkm-G6


title: nkm-G5


title: nkm-G1


title: nkm-F7


title: nkm-F5


title: nkm-F6


title: e8.5_V_V-A7


title: e8.5_V_V-A5


title: e8.5_V_V-A8


title: nkm-F4


title: nkm-E7


title: nkm-F2


title: nkm-F1


title: nkm-F3


title: nkm-G7


title: nkm-E4


title: nkm-E1


title: nkm-E6


title: nkm-D6


title: nkm-D7


title: nkm-E2


title: nkm-E5


title: nkm-D4


title: nkm-D3


title: nkm-E3


title: nkm-D5


title: nkm-G4


title: nkm-G2


title: nkm-G3


title: nkm-C6


title: nkm-C2


title: nkm-C1


title: nkm-C3


title: nkm-D2


title: nkm-C4


title: nkm-B7


title: nkm-C5


title: nkm-B4


title: nkm-B5


title: nkm-A5


title: nkm-A7


title: nkm-B3


title: nkm-B6


title: nkm-A6


title: nkm-B2


title: nkm-A3


title: lcm-H6

source_name: e10.5 LCM


title: nkm-A1


title: nkm-D1


title: lcm-H5


title: lcm-H4


title: lcm-G5


title: lcm-H2


title: lcm-H3


title: lcm-G6


title: nkm-A2


title: lcm-G4


title: lcm-G3


title: lcm-G2


title: lcm-F6


title: nkm-A4


title: lcm-F4


title: lcm-F3


title: lcm-E6


title: lcm-E1


title: lcm-D6


title: lcm-E4


title: lcm-F2


title: lcm-F1


title: nkm-C7


title: lcm-E2


title: lcm-D4


title: lcm-D5


title: lcm-D2


title: lcm-D3


title: lcm-C6


title: lcm-D1


title: lcm-C3


title: lcm-C4


title: lcm-C2


title: lcm-C5


title: lcm-C1


title: lcm-B4


title: lcm-A6


title: lcm-F5


title: lcm-B3


title: lcm-E5


title: lcm-A5


title: lcm-A4


title: lcm-B6


title: e8.5_A_A-H4

source_name: e8.5 atrium

value: atrium


title: e8.5_A_A-H6


title: e8.5_A_A-H9


title: e8.5_A_A-H7


title: e8.5_A_A-H8


title: lcm-A3


title: e8.5_A_A-H3


title: e8.5_A_A-H2


title: e8.5_A_A-G6


title: e8.5_A_A-G5


title: e8.5_A_A-H12


title: e8.5_A_A-G4


title: lcm-B5


title: e8.5_A_A-H10


title: e8.5_A_A-G3


title: e8.5_A_A-G2


title: e8.5_A_A-G12


title: e8.5_A_A-F9


title: e8.5_A_A-G1


title: e8.5_A_A-F5


title: e8.5_A_A-F8


title: e8.5_A_A-F1


title: e8.5_A_A-F6


title: e8.5_A_A-F2


title: e8.5_A_A-E4


title: e8.5_A_A-F10


title: e8.5_A_A-E11


title: e8.5_A_A-F12


title: e8.5_A_A-D9


title: e8.5_A_A-E12


title: e8.5_A_A-D7


title: e8.5_A_A-D2


title: e8.5_A_A-D6


title: e8.5_A_A-D3


title: e8.5_A_A-D4


title: e8.5_A_A-E7


title: e8.5_A_A-D11


title: e8.5_A_A-E3


title: e8.5_A_A-E9


title: e8.5_A_A-D1


title: e8.5_A_A-C8


title: e8.5_A_A-D10


title: e8.5_A_A-C9


title: e8.5_A_A-E6


title: e8.5_A_A-E1


title: e8.5_A_A-E10


title: e8.5_A_A-D8


title: lcm-E3


title: e8.5_A_A-C5


title: e8.5_A_A-C3


title: e8.5_A_A-C11


title: e8.5_A_A-C7


title: e8.5_A_A-B8


title: e8.5_A_A-C4


title: e8.5_A_A-C12


title: e8.5_A_A-D12


title: e8.5_A_A-B9


title: e8.5_A_A-B4


title: e8.5_A_A-B12


title: e8.5_A_A-B10


title: e8.5_A_A-B1


title: e8.5_A_A-A9


title: e8.5_A_A-A8


title: e8.5_A_A-B2


title: e8.5_A_A-A6


title: e8.5_A_A-A5


title: e8.5_A_A-C6


title: e8.5_A_A-A2


title: e8.5_A_A-A11


title: e8.5_A_A-B7


title: e8.5_A_A-A4


title: e8.5_A_A-A12


title: e8.5_A_A-C10


title: e8.5_A_A-B5


title: e10.5_RA_9-H6

source_name: e10.5 right atrium


title: e10.5_RA_9-H8


title: e8.5_A_A-A1


title: e8.5_A_A-B3


title: e10.5_RA_9-H5


title: e10.5_RA_9-H11


title: e10.5_RA_9-H2


title: e10.5_RA_9-H1


title: e10.5_RA_9-H7


title: e10.5_RA_9-G8


title: e10.5_RA_9-G9


title: e10.5_RA_9-G6


title: e10.5_RA_9-G2


title: e10.5_RA_9-H10


title: e8.5_A_A-A7


title: e10.5_RA_9-G1


title: e10.5_RA_9-F5


title: e10.5_RA_9-G11


title: e10.5_RA_9-G10


title: e10.5_RA_9-F4


title: e10.5_RA_9-F3


title: e10.5_RA_9-F10


title: e10.5_RA_9-F2


title: e10.5_RA_9-F1


title: e10.5_RA_9-F11


title: e10.5_RA_9-E8


title: e10.5_RA_9-F12


title: e10.5_RA_9-E6


title: e10.5_RA_9-E1


title: e10.5_RA_9-E2


title: e10.5_RA_9-E11


title: e10.5_RA_9-E7


title: e10.5_RA_9-E4


title: e10.5_RA_9-D5


title: e10.5_RA_9-D6


title: e10.5_RA_9-D12


title: e10.5_RA_9-G5


title: e10.5_RA_9-D9


title: e10.5_RA_9-C9


title: e10.5_RA_9-D2


title: e10.5_RA_9-D10


title: e10.5_RA_9-E12


title: e10.5_RA_9-D1


title: e10.5_RA_9-C8


title: e10.5_RA_9-C5


title: e10.5_RA_9-C6


title: e10.5_RA_9-C3


title: e10.5_RA_9-C12


title: e10.5_RA_9-C10


title: e10.5_RA_9-D7


title: e10.5_RA_9-B7


title: e10.5_RA_9-B8


title: e10.5_RA_9-B9


title: e10.5_RA_9-C2


title: e10.5_RA_9-B5


title: e10.5_RA_9-B6


title: e10.5_RA_9-B10


title: e10.5_RA_9-C11


title: e10.5_RA_9-B1


title: e10.5_RA_9-A8


title: e10.5_RA_9-B3


title: e10.5_RA_9-B4


title: e10.5_RA_9-B2


title: e10.5_RA_9-B11


title: e10.5_RA_9-A9


title: e10.5_RA_9-B12


title: e10.5_RA_9-A5


title: e10.5_RA_9-A3


title: e10.5_RA_9-A6


title: e10.5_RA_9-C4


title: e10.5_RA_9-A4


title: e10.5_RA_9-A12


title: e10.5_RA_9-A2


title: e10.5_LA_8-H7

source_name: e10.5 left atrium


title: e10.5_RA_9-A11


title: e10.5_RA_9-A10


title: e10.5_LA_8-H6


title: e10.5_LA_8-H4


title: e10.5_RA_9-A1


title: e10.5_LA_8-H3


title: e10.5_LA_8-G7


title: e10.5_LA_8-G6


title: e10.5_LA_8-G5


title: e10.5_LA_8-G2


title: e10.5_LA_8-G12


title: e10.5_LA_8-H11


title: e10.5_LA_8-G10


title: e10.5_LA_8-G3


title: e10.5_LA_8-G11


title: e10.5_LA_8-F8


title: e10.5_LA_8-G1


title: e10.5_LA_8-F5


title: e10.5_LA_8-F9


title: e10.5_LA_8-F4


title: e10.5_LA_8-F2


title: e10.5_LA_8-F1


title: e10.5_LA_8-F12


title: e10.5_LA_8-E3


title: e10.5_LA_8-E4


title: e10.5_LA_8-E12


title: e10.5_LA_8-E6


title: e10.5_LA_8-F10


title: e10.5_LA_8-E10


title: e10.5_LA_8-D8


title: e10.5_LA_8-D6


title: e10.5_LA_8-D4


title: e10.5_LA_8-D5


title: e10.5_LA_8-D11


title: e10.5_LA_8-D3


title: e10.5_LA_8-C9


title: e10.5_LA_8-D10


title: e10.5_LA_8-C4


title: e10.5_LA_8-C8


title: e10.5_LA_8-C7


title: e10.5_LA_8-C3


title: e10.5_LA_8-C2


title: e10.5_LA_8-C5


title: e10.5_LA_8-C12


title: e10.5_LA_8-E8


title: e10.5_LA_8-C11


title: e10.5_LA_8-C1


title: e10.5_LA_8-B9


title: e10.5_LA_8-E11


title: e10.5_LA_8-C10


title: e10.5_LA_8-B8


title: e10.5_LA_8-B7


title: e10.5_LA_8-B6


title: e10.5_LA_8-B3


title: e10.5_LA_8-B5


title: e10.5_LA_8-D9


title: e10.5_LA_8-B2


title: e10.5_LA_8-D12


title: e10.5_LA_8-B11


title: e10.5_LA_8-B10


title: e10.5_LA_8-B1


title: e10.5_LA_8-A9


title: e10.5_LA_8-B12


title: e10.5_LA_8-A6


title: e10.5_LA_8-A8


title: e10.5_LA_8-A5


title: e10.5_LA_8-A4


title: e10.5_LA_8-A2


title: e10.5_LA_8-A3


title: e9.5_OFT_7-H5


title: e9.5_OFT_7-H6


title: e9.5_OFT_7-H7


title: e9.5_OFT_7-H3


title: e9.5_OFT_7-H8


title: e9.5_OFT_7-H2


title: e9.5_OFT_7-H11


title: e9.5_OFT_7-G9


title: e9.5_OFT_7-G7


title: e9.5_OFT_7-G5


title: e9.5_OFT_7-G4


title: e9.5_OFT_7-G3


title: e9.5_OFT_7-G11


title: e9.5_OFT_7-F9


title: e9.5_OFT_7-F7


title: e9.5_OFT_7-G2


title: e9.5_OFT_7-G12


title: e9.5_OFT_7-G10


title: e9.5_OFT_7-F6


title: e10.5_LA_8-A12


title: e9.5_OFT_7-F5


title: e9.5_OFT_7-F4


title: e10.5_LA_8-A11


title: e9.5_OFT_7-F12


title: e9.5_OFT_7-F11


title: e9.5_OFT_7-E8


title: e9.5_OFT_7-E9


title: e9.5_OFT_7-F10


title: e9.5_OFT_7-F1


title: e9.5_OFT_7-E5


title: e9.5_OFT_7-E7


title: e9.5_OFT_7-G8


title: e9.5_OFT_7-E4


title: e9.5_OFT_7-G6


title: e9.5_OFT_7-E6


title: e9.5_OFT_7-E12


title: e9.5_OFT_7-E2


title: e9.5_OFT_7-E11


title: e9.5_OFT_7-E1


title: e9.5_OFT_7-E10


title: e9.5_OFT_7-D9


title: e9.5_OFT_7-D8


title: e9.5_OFT_7-D7


title: e9.5_OFT_7-F3


title: e9.5_OFT_7-D6


title: e9.5_OFT_7-D12


title: e9.5_OFT_7-D2


title: e9.5_OFT_7-D3


title: e9.5_OFT_7-D11


title: e9.5_OFT_7-D10


title: e9.5_OFT_7-D1


title: e9.5_OFT_7-C9


title: e9.5_OFT_7-C7


title: e9.5_OFT_7-E3


title: e9.5_OFT_7-C4


title: e9.5_OFT_7-C3


title: e9.5_OFT_7-C5


title: e9.5_OFT_7-C12


title: e9.5_OFT_7-C6


title: e9.5_OFT_7-C2


title: e9.5_OFT_7-B6


title: e9.5_OFT_7-B7


title: e9.5_OFT_7-B9


title: e9.5_OFT_7-B8


title: e9.5_OFT_7-C8


title: e9.5_OFT_7-B4


title: e9.5_OFT_7-B5


title: e9.5_OFT_7-B3


title: e9.5_OFT_7-B11


title: e9.5_OFT_7-D5


title: e9.5_OFT_7-B12


title: e9.5_OFT_7-B10


title: e9.5_OFT_7-A9


title: e9.5_OFT_7-B1


title: e9.5_OFT_7-D4


title: e9.5_OFT_7-A8


title: e9.5_OFT_7-A5


title: e9.5_OFT_7-A4


title: e9.5_OFT_7-A7


title: e9.5_OFT_7-A11


title: e9.5_OFT_7-A12


title: e9.5_OFT_7-A1


title: e9.5_OFT_7-A10


title: e9.5_RS_6-H8


title: e9.5_RS_6-H7


title: e9.5_RS_6-H6


title: e9.5_RS_6-H5


title: e9.5_RS_6-H2


title: e9.5_RS_6-H4


title: e9.5_RS_6-H12


title: e9.5_RS_6-H1


title: e9.5_RS_6-H3


title: e9.5_RS_6-G6


title: e9.5_RS_6-G9


title: e9.5_RS_6-G3


title: e9.5_RS_6-G4


title: e9.5_RS_6-G5


title: e9.5_RS_6-G12


title: e9.5_RS_6-G11


title: e9.5_RS_6-G10


title: e9.5_OFT_7-A6


title: e9.5_RS_6-F9


title: e9.5_RS_6-F6


title: e9.5_OFT_7-A3


title: e9.5_RS_6-F7


title: e9.5_RS_6-F8


title: e9.5_RS_6-F4


title: e9.5_RS_6-F5


title: e9.5_RS_6-F2


title: e9.5_RS_6-H11


title: e9.5_RS_6-F10


title: e9.5_RS_6-F1


title: e9.5_RS_6-E9


title: e9.5_RS_6-F11


title: e9.5_RS_6-G8


title: e9.5_RS_6-F12


title: e9.5_RS_6-E8


title: e9.5_RS_6-E6


title: e9.5_RS_6-E5


title: e9.5_RS_6-E2


title: e9.5_RS_6-E4


title: e9.5_RS_6-E11


title: e9.5_RS_6-E10


title: e9.5_RS_6-E1


title: e9.5_RS_6-E3


title: e9.5_RS_6-E12


title: e9.5_RS_6-D9


title: e9.5_RS_6-D6


title: e9.5_RS_6-D8


title: e9.5_RS_6-D3


title: e9.5_RS_6-D4


title: e9.5_RS_6-G1


title: e9.5_RS_6-D12


title: e9.5_RS_6-D11


title: e9.5_RS_6-C7


title: e9.5_RS_6-D10


title: e9.5_RS_6-C3


title: e9.5_RS_6-C4


title: e9.5_RS_6-C11


title: e9.5_RS_6-C10


title: e9.5_RS_6-B8


title: e9.5_RS_6-B5


title: e9.5_RS_6-B9


title: e9.5_RS_6-B7


title: e9.5_RS_6-B4


title: e9.5_RS_6-B3


title: e9.5_RS_6-B12


title: e9.5_RS_6-B10


title: e9.5_RS_6-B6


title: e9.5_RS_6-B1


title: e9.5_RS_6-A6


title: e9.5_RS_6-A7


title: e9.5_RS_6-A5


title: e9.5_RS_6-A4


title: e9.5_RS_6-C1


title: e9.5_RS_6-A3


title: e9.5_RS_6-C8


title: e9.5_RS_6-D1


title: e9.5_RS_6-C12


title: e9.5_RS_6-C2


title: e9.5_RS_6-A12


title: e9.5_RS_6-A11


title: e9.5_RS_6-A2


title: e9.5_RS_6-A1


title: e9.5_RV_5-H6


title: e9.5_RS_6-C5


title: e9.5_RV_5-H5


title: e9.5_RV_5-H2


title: e9.5_RV_5-G8


title: e9.5_RV_5-H12


title: e9.5_RV_5-H11


title: e9.5_RV_5-H1


title: e9.5_RV_5-H10


title: e9.5_RV_5-G9


title: e9.5_RV_5-G4


title: e9.5_RV_5-G5


title: e9.5_RS_6-B2


title: e9.5_RV_5-G3


title: e9.5_RV_5-G6


title: e9.5_RV_5-G2


title: e9.5_RV_5-G12


title: e9.5_RV_5-G11


title: e9.5_RS_6-A10


title: e9.5_RV_5-F9


title: e9.5_RV_5-F7


title: e9.5_RV_5-F8


title: e9.5_RV_5-G10


title: e9.5_RV_5-G1


title: e9.5_RV_5-F5


title: e9.5_RV_5-H3


title: e9.5_RV_5-H4


title: e9.5_RV_5-F6


title: e9.5_RV_5-F4


title: e9.5_RV_5-F3


title: e9.5_RV_5-F2


title: e9.5_RV_5-H7


title: e9.5_RV_5-G7


title: e9.5_RV_5-F11


title: e9.5_RV_5-E2


title: e9.5_RV_5-E10


title: e9.5_RV_5-E11


title: e9.5_RV_5-F12


title: e9.5_RV_5-E7


title: e9.5_RV_5-E5


title: e9.5_RV_5-E3


title: e9.5_RV_5-E4


title: e9.5_RV_5-E9


title: e9.5_RV_5-F10


title: e9.5_RV_5-E1


title: e9.5_RV_5-D4


title: e9.5_RV_5-D5


title: e9.5_RV_5-D9


title: e9.5_RV_5-D8


title: e9.5_RV_5-D6


title: e9.5_RV_5-D12


title: e9.5_RV_5-E8


title: e9.5_RV_5-E12


title: e9.5_RV_5-C9


title: e9.5_RV_5-D10


title: e9.5_RV_5-D11


title: e9.5_RV_5-D3


title: e9.5_RV_5-F1


title: e9.5_RV_5-C7


title: e9.5_RV_5-C6


title: e9.5_RV_5-C8


title: e9.5_RV_5-C5


title: e9.5_RV_5-C10


title: e9.5_RS_6-B11


title: e9.5_RV_5-B9


title: e9.5_RV_5-C12


title: e9.5_RV_5-C4


title: e9.5_RV_5-B10


title: e9.5_RV_5-B5


title: e9.5_RV_5-B12


title: e9.5_RV_5-B11


title: e9.5_RV_5-C3


title: e9.5_RV_5-C1


title: e9.5_RV_5-B4


title: e9.5_RV_5-D1


title: e9.5_RV_5-A5


title: e9.5_RV_5-B8


title: e9.5_RV_5-A2


title: e9.5_RV_5-B7


title: e9.5_RV_5-A6


title: e9.5_RV_5-B1


title: e9.5_RV_5-B6


title: e9.5_RV_5-A11


title: e9.5_RV_5-B2


title: e9.5_RV_5-A8


title: e9.5_RV_5-A4


title: e9.5_RV_5-A10


title: e9.5_LS_4-H5


title: e9.5_RV_5-A1


title: e9.5_LS_4-H6


title: e9.5_RV_5-A7


title: e9.5_LS_4-H3


title: e9.5_LS_4-H11


title: e9.5_LS_4-H12


title: e9.5_LS_4-H4


title: e9.5_LS_4-G8


title: e9.5_LS_4-H1


title: e9.5_LS_4-G9


title: e9.5_LS_4-G3


title: e9.5_LS_4-H10


title: e9.5_LS_4-G5


title: e9.5_LS_4-F7


title: e9.5_LS_4-F8


title: e9.5_LS_4-G11


title: e9.5_LS_4-G12


title: e9.5_LS_4-G10


title: e9.5_LS_4-F3


title: e9.5_LS_4-F5


title: e9.5_RV_5-A3


title: e9.5_LS_4-G1


title: e9.5_LS_4-F4


title: e9.5_LS_4-F6


title: e9.5_LS_4-F12


title: e9.5_LS_4-F2


title: e9.5_LS_4-F11


title: e9.5_LS_4-F10


title: e9.5_LS_4-E8


title: e9.5_LS_4-E5


title: e9.5_LS_4-G7


title: e9.5_LS_4-E4


title: e9.5_LS_4-E3


title: e9.5_LS_4-E2


title: e9.5_LS_4-E11


title: e9.5_LS_4-E12


title: e9.5_LS_4-E10


title: e9.5_LS_4-E1


title: e9.5_LS_4-D9


title: e9.5_LS_4-D4


title: e9.5_LS_4-D3


title: e9.5_LS_4-D11


title: e9.5_LS_4-D2


title: e9.5_LS_4-C9


title: e9.5_LS_4-C8


title: e9.5_LS_4-C7


title: e9.5_LS_4-C5


title: e9.5_LS_4-D6


title: e9.5_LS_4-C3


title: e9.5_LS_4-C12


title: e9.5_LS_4-F1


title: e9.5_LS_4-C10


title: e9.5_LS_4-B9


title: e9.5_LS_4-C11


title: e9.5_LS_4-C1


title: e9.5_LS_4-C6


title: e9.5_LS_4-D12


title: e9.5_LS_4-B8


title: e9.5_LS_4-D5


title: e9.5_LS_4-B7


title: e9.5_LS_4-B2


title: e9.5_LS_4-C2


title: e9.5_LS_4-B6


title: e9.5_LS_4-B12


title: e9.5_LS_4-B11


title: e9.5_LS_4-B10


title: e9.5_LS_4-B1


title: e9.5_LS_4-A5


title: e9.5_LS_4-C4


title: e9.5_LS_4-A3


title: e9.5_LS_4-B4


title: e9.5_LS_4-A11


title: e9.5_LS_4-A10


title: e9.5_LV_3-H9


title: e9.5_LS_4-A7


title: e9.5_LS_4-A6


title: e9.5_LV_3-H3


title: e9.5_LV_3-H2


title: e9.5_LV_3-H6


title: e9.5_LV_3-H12


title: e9.5_LS_4-A8


title: e9.5_LV_3-H11


title: e9.5_LV_3-H8


title: e9.5_LV_3-G8


title: e9.5_LV_3-G6


title: e9.5_LS_4-A2


title: e9.5_LV_3-G12


title: e9.5_LV_3-F9


title: e9.5_LV_3-F8


title: e9.5_LV_3-G3


title: e9.5_LV_3-G10


title: e9.5_LV_3-G5


title: e9.5_LV_3-G1


title: e9.5_LS_4-A4


title: e9.5_LV_3-F7


title: e9.5_LV_3-F6


title: e9.5_LS_4-A1


title: e9.5_LV_3-F4


title: e9.5_LV_3-F5


title: e9.5_LV_3-H4


title: e9.5_LV_3-F3


title: e9.5_LV_3-F2


title: e9.5_LV_3-F12


title: e9.5_LV_3-F11


title: e9.5_LV_3-F10


title: e9.5_LV_3-F1


title: e9.5_LV_3-E3


title: e9.5_LV_3-E9


title: e9.5_LV_3-E8


title: e9.5_LV_3-E6


title: e9.5_LV_3-E2


title: e9.5_LV_3-G4


title: e9.5_LV_3-E12


title: e9.5_LV_3-E11


title: e9.5_LV_3-E10


title: e9.5_LV_3-D9


title: e9.5_LV_3-D7


title: e9.5_LV_3-D8


title: e9.5_LV_3-C9


title: e9.5_LV_3-D5


title: e9.5_LV_3-D11


title: e9.5_LV_3-D1


title: e9.5_LV_3-D2


title: e9.5_LV_3-D10


title: e9.5_LV_3-C8


title: e9.5_LV_3-D4


title: e9.5_LV_3-C7


title: e9.5_LV_3-C2


title: e9.5_LV_3-D12


title: e9.5_LV_3-C4


title: e9.5_LV_3-C12


title: e9.5_LV_3-C11


title: e9.5_LV_3-C10


title: e9.5_LV_3-B6


title: e9.5_LV_3-B8


title: e9.5_LV_3-B7


title: e9.5_LV_3-B5


title: e9.5_LV_3-B2


title: e9.5_LV_3-B3


title: e9.5_LV_3-B12


title: e9.5_LV_3-B4


title: e9.5_LV_3-B9


title: e9.5_LV_3-D6


title: e9.5_LV_3-A9


title: e9.5_LV_3-B10


title: e9.5_LV_3-C3


title: e9.5_LV_3-A6


title: e9.5_LV_3-A3


title: e9.5_LV_3-A4


title: e9.5_LV_3-A2


title: e9.5_LV_3-A5


title: e9.5_LV_3-A12


title: e9.5_LV_3-A10


title: e10.5_PO_29-H8

source_name: e10.5 proximal outflow tract

value: proximal outflow tract


title: e10.5_PO_29-H4


title: e10.5_PO_29-H5


title: e10.5_PO_29-H6


title: e10.5_PO_29-H3


title: e10.5_PO_29-H10


title: e10.5_PO_29-H12


title: e10.5_PO_29-H11


title: e10.5_PO_29-H1


title: e10.5_PO_29-G9


title: e10.5_PO_29-G8


title: e10.5_PO_29-H2


title: e9.5_LV_3-A8


title: e10.5_PO_29-G5


title: e10.5_PO_29-G10


title: e10.5_PO_29-G2


title: e10.5_PO_29-G1


title: e10.5_PO_29-G7


title: e9.5_LV_3-A11


title: e10.5_PO_29-F9


title: e9.5_LV_3-A1


title: e10.5_PO_29-H7


title: e10.5_PO_29-F8


title: e10.5_PO_29-F7


title: e10.5_PO_29-F6


title: e10.5_PO_29-F5


title: e10.5_PO_29-F3


title: e10.5_PO_29-F2


title: e10.5_PO_29-E6


title: e10.5_PO_29-F11


title: e10.5_PO_29-E3


title: e10.5_PO_29-E5


title: e10.5_PO_29-E4


title: e10.5_PO_29-E9


title: e10.5_PO_29-E7


title: e10.5_PO_29-E2


title: e10.5_PO_29-F1


title: e10.5_PO_29-E12


title: e10.5_PO_29-D8


title: e10.5_PO_29-D9


title: e10.5_PO_29-G3


title: e10.5_PO_29-E1


title: e10.5_PO_29-D5


title: e10.5_PO_29-D2


title: e10.5_PO_29-D6


title: e10.5_PO_29-D4


title: e10.5_PO_29-D12


title: e10.5_PO_29-C9


title: e10.5_PO_29-D11


title: e10.5_PO_29-D10


title: e10.5_PO_29-D3


title: e10.5_PO_29-D1


title: e10.5_PO_29-C8


title: e10.5_PO_29-C2


title: e10.5_PO_29-C6


title: e10.5_PO_29-C3


title: e10.5_PO_29-C12


title: e10.5_PO_29-C4


title: e10.5_PO_29-B8


title: e10.5_PO_29-B2


title: e10.5_PO_29-A9


title: e10.5_PO_29-B7


title: e10.5_PO_29-B4


title: e10.5_PO_29-B1


title: e10.5_PO_29-B9


title: e10.5_PO_29-B12


title: e10.5_PO_29-A8


title: e10.5_PO_29-A7


title: e10.5_PO_29-A6


title: e10.5_PO_29-A12


title: e10.5_PO_29-A11


title: e10.5_PO_29-A4


title: e10.5_PO_29-A2


title: e10.5_DO_28-H5

source_name: e10.5 distal outflow tract

value: distal outflow tract


title: e10.5_PO_29-A1


title: e10.5_DO_28-H7


title: e10.5_DO_28-H6


title: e10.5_DO_28-H12


title: e10.5_DO_28-H1


title: e10.5_DO_28-H2


title: e10.5_DO_28-H4


title: e10.5_DO_28-H11


title: e10.5_DO_28-H10


title: e10.5_DO_28-H3


title: e10.5_PO_29-B5


title: e10.5_PO_29-B3


title: e10.5_PO_29-C1


title: e10.5_PO_29-C11


title: e10.5_DO_28-G7


title: e10.5_DO_28-F8


title: e10.5_DO_28-F7


title: e10.5_DO_28-G1


title: e10.5_DO_28-G8


title: e10.5_DO_28-F3


title: e10.5_DO_28-G5


title: e10.5_DO_28-G11


title: e10.5_DO_28-F5


title: e10.5_DO_28-F11


title: e10.5_DO_28-F10


title: e10.5_DO_28-F1


title: e10.5_DO_28-F9


title: e10.5_DO_28-F2


title: e10.5_DO_28-E9


title: e10.5_DO_28-F12


title: e10.5_DO_28-E7


title: e10.5_DO_28-E4


title: e10.5_DO_28-E12


title: e10.5_DO_28-E3


title: e10.5_DO_28-E10


title: e10.5_DO_28-E5


title: e10.5_DO_28-E2


title: e10.5_DO_28-E1


title: e10.5_DO_28-D9


title: e10.5_DO_28-G6


title: e10.5_DO_28-G9


title: e10.5_DO_28-G4


title: e10.5_DO_28-G12


title: e10.5_DO_28-D8


title: e10.5_DO_28-D7


title: e10.5_DO_28-D6


title: e10.5_DO_28-D4


title: e10.5_DO_28-D12


title: e10.5_DO_28-D11


title: e10.5_DO_28-C8


title: e10.5_DO_28-E8


title: e10.5_DO_28-C6


title: e10.5_DO_28-C2


title: e10.5_DO_28-C11


title: e10.5_DO_28-C5


title: e10.5_DO_28-B9


title: e10.5_DO_28-B6


title: e10.5_DO_28-C3


title: e10.5_DO_28-B7


title: e10.5_DO_28-B4


title: e10.5_DO_28-C12


title: e10.5_DO_28-B5


title: e10.5_DO_28-B3


title: e10.5_DO_28-B1


title: e10.5_DO_28-B12


title: e10.5_DO_28-A4


title: e10.5_DO_28-A5


title: e10.5_DO_28-A9


title: e10.5_DO_28-A6


title: e10.5_DO_28-A7


title: e10.5_DO_28-A2


title: e10.5_DO_28-D2


title: e10.5_DO_28-D1


title: e10.5_DO_28-A11


title: e10.5_DO_28-A1


title: e10.5_LV_27-H7

source_name: e10.5 left ventricle


title: e10.5_LV_27-H6


title: e10.5_LV_27-H12


title: e10.5_LV_27-H4


title: e10.5_LV_27-G7


title: e10.5_LV_27-G4


title: e10.5_LV_27-G2


title: e10.5_LV_27-G10


title: e10.5_LV_27-H11


title: e10.5_DO_28-B11


title: e10.5_LV_27-G12


title: e10.5_LV_27-F8


title: e10.5_LV_27-G5


title: e10.5_LV_27-F7


title: e10.5_LV_27-F11


title: e10.5_LV_27-F12


title: e10.5_LV_27-F2


title: e10.5_LV_27-F5


title: e10.5_LV_27-E9


title: e10.5_LV_27-G6


title: e10.5_DO_28-A12


title: e10.5_LV_27-E7


title: e10.5_LV_27-F10


title: e10.5_LV_27-E5


title: e10.5_LV_27-E4


title: e10.5_LV_27-E12


title: e10.5_LV_27-E3


title: e10.5_LV_27-E10


title: e10.5_LV_27-E1


title: e10.5_LV_27-D9


title: e10.5_LV_27-G1


title: e10.5_LV_27-D5


title: e10.5_LV_27-D8


title: e10.5_LV_27-D3


title: e10.5_LV_27-D2


title: e10.5_LV_27-D12


title: e10.5_LV_27-C8


title: e10.5_LV_27-D10


title: e10.5_LV_27-D11


title: e10.5_LV_27-C7


title: e10.5_LV_27-C9


title: e10.5_LV_27-C4


title: e10.5_LV_27-D1


title: e10.5_LV_27-B6


title: e10.5_LV_27-C2


title: e10.5_LV_27-B5


title: e10.5_LV_27-C10


title: e10.5_LV_27-B4


title: e10.5_LV_27-C11


title: e10.5_LV_27-C3


title: e10.5_LV_27-C12


title: e10.5_LV_27-B11


title: e10.5_LV_27-B3


title: e10.5_LV_27-B7


title: e10.5_LV_27-D4


title: e10.5_LV_27-A7


title: e10.5_LV_27-A9


title: e10.5_LV_27-A8


title: e10.5_LV_27-A4


title: e10.5_LV_27-B1


title: e10.5_LV_27-A5


title: e10.5_LV_27-A3


title: e10.5_LV_27-A11


title: e10.5_LV_27-A1


title: e10.5_AVC_26-H7

source_name: e10.5 atrioventricular canal

value: atrioventricular canal


title: e10.5_AVC_26-H8


title: e10.5_AVC_26-H6


title: e10.5_AVC_26-G9


title: e10.5_AVC_26-H10


title: e10.5_AVC_26-H11


title: e10.5_AVC_26-H5


title: e10.5_AVC_26-G8


title: e10.5_AVC_26-H3


title: e10.5_AVC_26-G7


title: e10.5_AVC_26-G6


title: e10.5_LV_27-A6


title: e10.5_AVC_26-G5


title: e10.5_AVC_26-G1


title: e10.5_AVC_26-G11


title: e10.5_AVC_26-G12


title: e10.5_AVC_26-F8


title: e10.5_AVC_26-G2


title: e10.5_LV_27-A2


title: e10.5_AVC_26-F7


title: e10.5_AVC_26-F6


title: e10.5_LV_27-A10


title: e10.5_AVC_26-F5


title: e10.5_AVC_26-F12


title: e10.5_AVC_26-F11


title: e10.5_AVC_26-F2


title: e10.5_AVC_26-F1


title: e10.5_AVC_26-F10


title: e10.5_AVC_26-E9


title: e10.5_AVC_26-E4


title: e10.5_AVC_26-E5


title: e10.5_AVC_26-E2


title: e10.5_AVC_26-E12


title: e10.5_AVC_26-E11


title: e10.5_AVC_26-E1


title: e10.5_AVC_26-E10


title: e10.5_AVC_26-D7


title: e10.5_AVC_26-D6


title: e10.5_AVC_26-D8


title: e10.5_AVC_26-D5


title: e10.5_AVC_26-D4


title: e10.5_AVC_26-D2


title: e10.5_AVC_26-D11


title: e10.5_AVC_26-F3


title: e10.5_AVC_26-D10


title: e10.5_AVC_26-C8


title: e10.5_AVC_26-C9


title: e10.5_AVC_26-D9


title: e10.5_AVC_26-C6


title: e10.5_AVC_26-C5


title: e10.5_AVC_26-C3


title: e10.5_AVC_26-D1


title: e10.5_AVC_26-C11


title: e10.5_AVC_26-C2


title: e10.5_AVC_26-E6


title: e10.5_AVC_26-B8


title: e10.5_AVC_26-B9


title: e10.5_AVC_26-B7


title: e10.5_AVC_26-D12


title: e10.5_AVC_26-B5


title: e10.5_AVC_26-B6


title: e10.5_AVC_26-B3


title: e10.5_AVC_26-B12


title: e10.5_AVC_26-B10


title: e10.5_AVC_26-A5


title: e10.5_AVC_26-B11


title: e10.5_AVC_26-A3


title: e10.5_AVC_26-D3


title: e10.5_AVC_26-A4


title: e10.5_AVC_26-A9


title: e10.5_AVC_26-A7


title: e10.5_AVC_26-A6


title: e10.5_AVC_26-A12


title: e10.5_AVC_26-C1


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-H5

source_name: e10.5 Isl1-Cre; ROSA26-mTmG GFP pos

value: CD1/C57BL/6 mixed


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-H8


title: e10.5_AVC_26-A10


title: e10.5_AVC_26-A2


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-H6


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-H4


title: e10.5_AVC_26-A11


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-H11


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-H7


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-H10


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-H1


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-G8


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-G9


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-G6


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-G7


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-G4


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-G5


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-G3


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-G11


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-G12


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-F9


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-H2


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-F4


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-F7


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-F8


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-F3


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-F5


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-F6


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-F2


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-E9


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-F10


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-F12


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-E7


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-E8


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-E6


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-F11


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-E4


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-E2


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-E5


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-E1


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-D8


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-G1


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-D5


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-D3


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-D6


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-D11


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-D12


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-D10


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-C8


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-C5


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-C9


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-C4


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-D1


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-D4


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-C3


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-D9


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-E12


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-C12


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-C1


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-E11


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-E10


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-C10


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-C11


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-B8


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-B7


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-B9


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-D7


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-B5


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-B6


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-B4


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-B2


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-B12


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-B11


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-B10


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-C2


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-A8


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-A7


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-A9


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-A6


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-A4


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-A3


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-G3

source_name: e10.5 Isl1-Cre; ROSA26-mTmG GFP neg


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-G4


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-H8


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-A2


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-G5


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-A10


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-H11


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-A11


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-H7


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-G8


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-H2


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-H1


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-G7


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-G9


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-A12


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-H5


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-H10


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-H4


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-H3


title: Isl1-Cre; ROSA26-mTmG_GFP_pos_25-A1


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-G6


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-H6


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-G2


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-G12


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-F7


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-G10


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-F6


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-F9


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-E5


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-E3


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-E7


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-G1


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-D8


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-D4


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-F12


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-F1


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-E6


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-F8


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-F11


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-E10


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-F3


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-E12


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-E11


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-D7


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-E2


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-E8


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-E9


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-D3


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-D12


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-D9


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-D11


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-D1


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-D10


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-F5


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-F4


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-E4


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-D2


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-C9


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-C2


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-C8


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-C3


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-C11


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-C12


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-B9


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-B6


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-C1


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-B7


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-A8


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-B3


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-B12


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-A7


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-A6


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-B5


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-B11


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-B2


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-B8


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-B4


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-A9


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-A2


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-A3


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-A12


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-A10


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-A5


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-A11


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-A1


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-C6


title: Isl1-Cre; ROSA26-mTmG_GFP_neg_24-B10


title: e10.5_DO_23-H2


title: e10.5_DO_23-H5


title: e10.5_DO_23-H4


title: e10.5_DO_23-H6


title: e10.5_DO_23-H10


title: e10.5_DO_23-G9


title: e10.5_DO_23-G8


title: e10.5_DO_23-G11


title: e10.5_DO_23-G4


title: e10.5_DO_23-G12


title: e10.5_DO_23-F2


title: e10.5_DO_23-F12


title: e10.5_DO_23-G7


title: e10.5_DO_23-F9


title: e10.5_DO_23-G10


title: e10.5_DO_23-G6


title: e10.5_DO_23-F1


title: e10.5_DO_23-G2


title: e10.5_DO_23-G5


title: e10.5_DO_23-F5


title: e10.5_DO_23-E6


title: e10.5_DO_23-F10


title: e10.5_DO_23-E8


title: e10.5_DO_23-E4


title: e10.5_DO_23-E5


title: e10.5_DO_23-H12


title: e10.5_DO_23-E3


title: e10.5_DO_23-H11


title: e10.5_DO_23-E12


title: e10.5_DO_23-E10


title: e10.5_DO_23-E1


title: e10.5_DO_23-D8


title: e10.5_DO_23-F4


title: e10.5_DO_23-F7


title: e10.5_DO_23-D4


title: e10.5_DO_23-D7


title: e10.5_DO_23-D2


title: e10.5_DO_23-D10


title: e10.5_DO_23-D1


title: e10.5_DO_23-C7


title: e10.5_DO_23-D12


title: e10.5_DO_23-C6


title: e10.5_DO_23-D11


title: e10.5_DO_23-C9


title: e10.5_DO_23-C4


title: e10.5_DO_23-C10


title: e10.5_DO_23-C1


title: e10.5_DO_23-C11


title: e10.5_DO_23-C2


title: e10.5_DO_23-B9


title: e10.5_DO_23-B8


title: e10.5_DO_23-B7


title: e10.5_DO_23-C12


title: e10.5_DO_23-E2


title: e10.5_DO_23-B5


title: e10.5_DO_23-B6


title: e10.5_DO_23-B4


title: e10.5_DO_23-B2


title: e10.5_DO_23-B3


title: e10.5_DO_23-A9


title: e10.5_DO_23-A8


title: e10.5_DO_23-A5


title: e10.5_DO_23-B10


title: e10.5_PO_22-H6


title: e10.5_PO_22-H2


title: e10.5_PO_22-H3


title: e10.5_PO_22-H1


title: e10.5_DO_23-A1


title: e10.5_DO_23-A2


title: e10.5_PO_22-G9


title: e10.5_PO_22-H7


title: e10.5_PO_22-H4


title: e10.5_PO_22-G5


title: e10.5_PO_22-G10


title: e10.5_DO_23-B12


title: e10.5_PO_22-H12


title: e10.5_PO_22-G1


title: e10.5_PO_22-F7


title: e10.5_PO_22-F5


title: e10.5_PO_22-F2


title: e10.5_PO_22-H11


title: e10.5_PO_22-G2


title: e10.5_PO_22-F1


title: e10.5_PO_22-G7


title: e10.5_PO_22-F10


title: e10.5_PO_22-F9


title: e10.5_PO_22-G8


title: e10.5_PO_22-F8


title: e10.5_PO_22-F6


title: e10.5_PO_22-E7


title: e10.5_PO_22-E1


title: e10.5_PO_22-E11


title: e10.5_PO_22-E3


title: e10.5_PO_22-E10


title: e10.5_PO_22-F11


title: e10.5_PO_22-H5


title: e10.5_PO_22-G4


title: e10.5_PO_22-H10


title: e10.5_PO_22-E9


title: e10.5_PO_22-E8


title: e10.5_PO_22-E6


title: e10.5_PO_22-F3


title: e10.5_PO_22-D12


title: e10.5_PO_22-D11


title: e10.5_PO_22-C8


title: e10.5_PO_22-E5


title: e10.5_PO_22-C3


title: e10.5_PO_22-E4


title: e10.5_PO_22-E2


title: e10.5_PO_22-D8


title: e10.5_PO_22-D9


title: e10.5_PO_22-D6


title: e10.5_PO_22-D5


title: e10.5_PO_22-D4


title: e10.5_PO_22-B6


title: e10.5_PO_22-D2


title: e10.5_PO_22-D10


title: e10.5_PO_22-C9


title: e10.5_PO_22-C5


title: e10.5_PO_22-C11


title: e10.5_PO_22-C12


title: e10.5_PO_22-B10


title: e10.5_PO_22-A9


title: e10.5_PO_22-C10


title: e10.5_PO_22-A2


title: e10.5_PO_22-A12


title: e10.5_PO_22-B7


title: e10.5_PO_22-B9


title: e10.5_PO_22-B5


title: e10.5_PO_22-B3


title: e10.5_PO_22-B12


title: e10.5_PO_22-B11


title: e10.5_PO_22-A7


title: e10.5_PO_22-A6


title: e10.5_PO_22-A3


title: e10.5_RS_21-G3

source_name: e10.5 right ventricular septum


title: e10.5_RS_21-G12


title: e10.5_RS_21-H4


title: e10.5_RS_21-G11


title: e10.5_RS_21-H12


title: e10.5_RS_21-H11


title: e10.5_PO_22-B8


title: e10.5_RS_21-F9


title: e10.5_RS_21-H1


title: e10.5_RS_21-G10


title: e10.5_RS_21-F7


title: e10.5_RS_21-G7


title: e10.5_PO_22-B2


title: e10.5_RS_21-F11


title: e10.5_RS_21-G9


title: e10.5_RS_21-F8


title: e10.5_RS_21-E10


title: e10.5_RS_21-G6


title: e10.5_RS_21-F6


title: e10.5_PO_22-B1


title: e10.5_RS_21-G5


title: e10.5_PO_22-A8


title: e10.5_RS_21-G1


title: e10.5_RS_21-F4


title: e10.5_RS_21-D7


title: e10.5_RS_21-F3


title: e10.5_RS_21-D4


title: e10.5_RS_21-D12


title: e10.5_RS_21-D1


title: e10.5_RS_21-D3


title: e10.5_RS_21-C7


title: e10.5_RS_21-C6


title: e10.5_RS_21-E9


title: e10.5_RS_21-D11


title: e10.5_RS_21-E6


title: e10.5_RS_21-D9


title: e10.5_RS_21-C4


title: e10.5_RS_21-C5


title: e10.5_RS_21-C3


title: e10.5_RS_21-E1


title: e10.5_RS_21-C2


title: e10.5_RS_21-B7


title: e10.5_RS_21-B8


title: e10.5_RS_21-B9


title: e10.5_RS_21-C12


title: e10.5_RS_21-C10


title: e10.5_RS_21-B5


title: e10.5_RS_21-D6


title: e10.5_RS_21-B2


title: e10.5_RS_21-B6


title: e10.5_RS_21-C1


title: e10.5_RS_21-B12


title: e10.5_RS_21-A7


title: e10.5_RS_21-B1


title: e10.5_RS_21-B4


title: e10.5_RS_21-A9


title: e10.5_RS_21-A8


title: e10.5_RS_21-A4


title: e10.5_RS_21-A3


title: e10.5_RS_21-A5


title: e10.5_RS_21-A2


title: e10.5_RS_21-A11


title: e10.5_RS_21-E2


title: e10.5_RV_20-H8

source_name: e10.5 right ventricle


title: e10.5_RV_20-H3


title: e10.5_RS_21-B3


title: e10.5_RV_20-H12


title: e10.5_RS_21-A1


title: e10.5_RV_20-H1


title: e10.5_RV_20-H4


title: e10.5_RV_20-H2


title: e10.5_RV_20-G8


title: e10.5_RV_20-G6


title: e10.5_RV_20-G3


title: e10.5_RV_20-G2


title: e10.5_RS_21-A6


title: e10.5_RV_20-G11


title: e10.5_RV_20-F1


title: e10.5_RV_20-E3


title: e10.5_RV_20-E4


title: e10.5_RV_20-F8


title: e10.5_RV_20-F5


title: e10.5_RV_20-F12


title: e10.5_RV_20-F2


title: e10.5_RV_20-E10


title: e10.5_RV_20-E9


title: e10.5_RV_20-E7


title: e10.5_RV_20-D3


title: e10.5_RV_20-D9


title: e10.5_RV_20-G10


title: e10.5_RV_20-E11


title: e10.5_RV_20-D5


title: e10.5_RV_20-H10


title: e10.5_RV_20-E5


title: e10.5_RV_20-C5


title: e10.5_RV_20-C8


title: e10.5_RV_20-D2


title: e10.5_RV_20-D10


title: e10.5_RV_20-B5


title: e10.5_RV_20-D1


title: e10.5_RV_20-B8


title: e10.5_RV_20-C4


title: e10.5_RV_20-B2


title: e10.5_RV_20-C12


title: e10.5_RV_20-B11


title: e10.5_RV_20-C11


title: e10.5_RV_20-C10


title: e10.5_RV_20-B12


title: e10.5_RV_20-A9


title: e10.5_RV_20-C1


title: e10.5_RV_20-E1


title: e10.5_RV_20-B4


title: e10.5_RV_20-A2


title: e10.5_RV_20-B3


title: e10.5_RV_20-A11


title: e10.5_RV_20-B10


title: e10.5_RV_20-A3


title: e10.5_RV_20-A8


title: e10.5_RV_20-A10


title: e10.5_RV_20-A5


title: e10.5_RV_20-A6


title: e9.5_RA_2-H2

source_name: e9.5 right atrium

value: right atrium


title: e9.5_RA_2-H5


title: e9.5_RA_2-H12


title: e10.5_RV_20-B1


title: e10.5_RV_20-A4


title: e9.5_RA_2-H10


title: e9.5_RA_2-H11


title: e9.5_RA_2-H1


title: e9.5_RA_2-G8


title: e9.5_RA_2-G9


title: e9.5_RA_2-H7


title: e9.5_RA_2-G7


title: e9.5_RA_2-H4


title: e9.5_RA_2-H6


title: e10.5_RV_20-A12


title: e9.5_RA_2-G6


title: e9.5_RA_2-G4


title: e9.5_RA_2-G5


title: e9.5_RA_2-G2


title: e9.5_RA_2-G1


title: e9.5_RA_2-F7


title: e9.5_RA_2-G10


title: e9.5_RA_2-F5


title: e9.5_RA_2-F2


title: e9.5_RA_2-F12


title: e10.5_RV_20-A1


title: e9.5_RA_2-F11


title: e9.5_RA_2-F10


title: e9.5_RA_2-E10


title: e9.5_RA_2-E3


title: e9.5_RA_2-D6


title: e9.5_RA_2-E6


title: e9.5_RA_2-E5


title: e9.5_RA_2-E4


title: e9.5_RA_2-G12


title: e9.5_RA_2-D5


title: e9.5_RA_2-E8


title: e9.5_RA_2-E12


title: e9.5_RA_2-E1


title: e9.5_RA_2-G11


title: e9.5_RA_2-E11


title: e9.5_RA_2-D3


title: e9.5_RA_2-D4


title: e9.5_RA_2-D2


title: e9.5_RA_2-D1


title: e9.5_RA_2-C9


title: e9.5_RA_2-C7


title: e9.5_RA_2-C4


title: e9.5_RA_2-C6


title: e9.5_RA_2-C2


title: e9.5_RA_2-C5


title: e9.5_RA_2-C11


title: e9.5_RA_2-F1


title: e9.5_RA_2-C12


title: e9.5_RA_2-D8


title: e9.5_RA_2-B8


title: e9.5_RA_2-D12


title: e9.5_RA_2-D11


title: e9.5_RA_2-C10


title: e9.5_RA_2-B6


title: e9.5_RA_2-A8


title: e9.5_RA_2-B2


title: e9.5_RA_2-A3


title: e9.5_RA_2-A7


title: e9.5_RA_2-B4


title: e9.5_RA_2-A2


title: e9.5_RA_2-B5


title: e9.5_RA_2-C3


title: e9.5_RA_2-A6


title: e9.5_RA_2-A10


title: e9.5_RA_2-A9


title: e9.5_RA_2-B3


title: e9.5_RA_2-A1


title: e10.5_LS_19-H7

source_name: e10.5 left ventricular septum


title: e10.5_LS_19-H2


title: e10.5_LS_19-H6


title: e10.5_LS_19-H12


title: e10.5_LS_19-H11


title: e10.5_LS_19-H1


title: e10.5_LS_19-H5


title: e10.5_LS_19-G9


title: e10.5_LS_19-G7


title: e9.5_RA_2-C1


title: e9.5_RA_2-A4


title: e10.5_LS_19-G2


title: e10.5_LS_19-G10


title: e10.5_LS_19-G4


title: e10.5_LS_19-G1


title: e10.5_LS_19-F9


title: e10.5_LS_19-F7


title: e10.5_LS_19-F3


title: e10.5_LS_19-E12


title: e10.5_LS_19-F11


title: e10.5_LS_19-F12


title: e10.5_LS_19-F4


title: e10.5_LS_19-E8


title: e10.5_LS_19-F5


title: e10.5_LS_19-E10


title: e10.5_LS_19-E2


title: e10.5_LS_19-E4


title: e10.5_LS_19-E5


title: e10.5_LS_19-E1


title: e10.5_LS_19-D9


title: e10.5_LS_19-G8


title: e10.5_LS_19-E3


title: e10.5_LS_19-G5


title: e10.5_LS_19-E11


title: e10.5_LS_19-D7


title: e10.5_LS_19-D6


title: e10.5_LS_19-D3


title: e10.5_LS_19-D4


title: e10.5_LS_19-D5


title: e10.5_LS_19-D2


title: e10.5_LS_19-D12


title: e10.5_LS_19-D1


title: e10.5_LS_19-D11


title: e10.5_LS_19-D8


title: e10.5_LS_19-C4


title: e10.5_LS_19-C3


title: e10.5_LS_19-D10


title: e10.5_LS_19-C9


title: e10.5_LS_19-C1


title: e10.5_LS_19-C7


title: e10.5_LS_19-C2


title: e10.5_LS_19-C10


title: e10.5_LS_19-A9


title: e10.5_LS_19-B6


title: e10.5_LS_19-B12


title: e10.5_LS_19-B7


title: e10.5_LS_19-A8


title: e10.5_LS_19-G3


title: e10.5_LS_19-A4


title: e10.5_LS_19-A6


title: e10.5_LS_19-A7


title: e10.5_LS_19-A2


title: e10.5_LV_18-H6

value: left ventricle


title: e10.5_LS_19-A11


title: e10.5_LS_19-A3


title: e10.5_LV_18-H5


title: e10.5_LV_18-H2


title: e10.5_LS_19-A5


title: e10.5_LV_18-H11


title: e10.5_LS_19-B9


title: e10.5_LV_18-H10


title: e10.5_LV_18-G4


title: e10.5_LV_18-G8


title: e10.5_LV_18-G7


title: e10.5_LV_18-G9


title: e10.5_LV_18-G6


title: e10.5_LV_18-H1


title: e10.5_LV_18-G3


title: e10.5_LV_18-G10


title: e10.5_LV_18-F2


title: e10.5_LV_18-F11


title: e10.5_LV_18-F6


title: e10.5_LV_18-G2


title: e10.5_LV_18-E8


title: e10.5_LV_18-E7


title: e10.5_LV_18-F7


title: e10.5_LV_18-F9


title: e10.5_LV_18-E2


title: e10.5_LV_18-E11


title: e10.5_LV_18-E5


title: e10.5_LV_18-F5


title: e10.5_LV_18-G11


title: e10.5_LV_18-F4


title: e10.5_LV_18-F10


title: e10.5_LV_18-H12


title: e10.5_LV_18-D1


title: e10.5_LV_18-D3


title: e10.5_LV_18-F1


title: e10.5_LV_18-E6


title: e10.5_LV_18-D8


title: e10.5_LV_18-D5


title: e10.5_LV_18-E9


title: e10.5_LV_18-C3


title: e10.5_LV_18-C5


title: e10.5_LV_18-C4


title: e10.5_LV_18-E4


title: e10.5_LV_18-E12


title: e10.5_LV_18-D11


title: e10.5_LV_18-C7


title: e10.5_LV_18-C8


title: e10.5_LV_18-B5


title: e10.5_LV_18-B12


title: e10.5_LV_18-B4


title: e10.5_LV_18-B7


title: e10.5_LV_18-C6


title: e10.5_LV_18-B2


title: e10.5_LV_18-B11


title: e10.5_LV_18-A9


title: e10.5_LV_18-A6


title: e10.5_LV_18-C2


title: e10.5_RA_17-H8


title: e10.5_LV_18-C10


title: e10.5_RA_17-H4


title: e10.5_LV_18-C1


title: e10.5_LV_18-B10


title: e10.5_LV_18-B6


title: e10.5_LV_18-B8


title: e10.5_LV_18-A7


title: e10.5_RA_17-H2


title: e10.5_LV_18-A8


title: e10.5_LV_18-D6


title: e10.5_LV_18-A12


title: e10.5_RA_17-H1


title: e10.5_LV_18-A2


title: e10.5_LV_18-B3


title: e10.5_LV_18-A4


title: e10.5_RA_17-G8


title: e10.5_LV_18-A3


title: e10.5_LV_18-A1


title: e10.5_RA_17-H5


title: e10.5_RA_17-G12


title: e10.5_RA_17-H12


title: e10.5_RA_17-H10


title: e10.5_RA_17-H11


title: e10.5_RA_17-F9


title: e10.5_RA_17-F7


title: e10.5_RA_17-G5


title: e10.5_RA_17-G3


title: e10.5_RA_17-G6


title: e10.5_RA_17-F5


title: e10.5_RA_17-F6


title: e10.5_RA_17-F8


title: e10.5_RA_17-F3


title: e10.5_RA_17-F4


title: e10.5_RA_17-F11


title: e10.5_RA_17-F2


title: e10.5_RA_17-G2


title: e10.5_RA_17-G7


title: e10.5_RA_17-F12


title: e10.5_RA_17-E8


title: e10.5_RA_17-E9


title: e10.5_RA_17-F10


title: e10.5_RA_17-E2


title: e10.5_RA_17-E11


title: e10.5_RA_17-E10


title: e10.5_RA_17-E5


title: e10.5_RA_17-D4


title: e10.5_RA_17-D6


title: e10.5_RA_17-D2


title: e10.5_RA_17-D9


title: e10.5_RA_17-D12


title: e10.5_RA_17-C12


title: e10.5_RA_17-C6


title: e10.5_RA_17-C9


title: e10.5_RA_17-C8


title: e10.5_RA_17-D11


title: e10.5_RA_17-C5


title: e10.5_RA_17-B4


title: e10.5_RA_17-B8


title: e10.5_RA_17-E12


title: e10.5_RA_17-E4


title: e10.5_RA_17-B3


title: e10.5_RA_17-B5


title: e10.5_RA_17-B6


title: e10.5_RA_17-B7


title: e10.5_RA_17-B2


title: e10.5_RA_17-B12


title: e10.5_RA_17-B11


title: e10.5_RA_17-B10


title: e10.5_RA_17-D7


title: e10.5_RA_17-A7


title: e10.5_RA_17-A9


title: e10.5_RA_17-A4


title: e10.5_RA_17-A12


title: e10.5_RA_17-C11


title: e10.5_RA_17-A2


title: e10.5_RA_17-A10


title: e10.5_LA_16-H5


title: e10.5_LA_16-H6


title: e10.5_RA_17-A11


title: e10.5_LA_16-H2


title: e10.5_LA_16-H3


title: e10.5_LA_16-H4


title: e10.5_LA_16-H11


title: e10.5_LA_16-G4


title: e10.5_LA_16-G7


title: e10.5_LA_16-G11


title: e10.5_LA_16-G5


title: e10.5_LA_16-G6


title: e10.5_LA_16-G12


title: e10.5_LA_16-G10


title: e10.5_LA_16-F3


title: e10.5_LA_16-G1


title: e10.5_LA_16-F12


title: e10.5_LA_16-F1


title: e10.5_LA_16-F11


title: e10.5_LA_16-F9


title: e10.5_LA_16-F6


title: e10.5_LA_16-E9


title: e10.5_RA_17-A3


title: e10.5_LA_16-G9


title: e10.5_LA_16-E2


title: e10.5_LA_16-E8


title: e10.5_LA_16-E5


title: e10.5_LA_16-E6


title: e10.5_LA_16-E12


title: e10.5_LA_16-G8


title: e10.5_LA_16-E3


title: e10.5_LA_16-E7


title: e10.5_LA_16-F8


title: e10.5_LA_16-F10


title: e10.5_LA_16-E10


title: e10.5_LA_16-D6


title: e10.5_LA_16-E1


title: e10.5_LA_16-D9


title: e10.5_LA_16-D11


title: e10.5_LA_16-D5


title: e10.5_LA_16-C6


title: e10.5_LA_16-D4


title: e10.5_LA_16-C4


title: e10.5_LA_16-D2


title: e10.5_LA_16-D8


title: e10.5_LA_16-D12


title: e10.5_LA_16-C9


title: e10.5_LA_16-D10


title: e10.5_LA_16-D7


title: e10.5_LA_16-B9


title: e10.5_LA_16-C1


title: e10.5_LA_16-B8


title: e10.5_LA_16-C8


title: e10.5_LA_16-C7


title: e10.5_LA_16-B3


title: e10.5_LA_16-B1


title: e10.5_LA_16-C3


title: e10.5_LA_16-A9


title: e10.5_LA_16-A8


title: e10.5_LA_16-C2


title: e10.5_LA_16-B7


title: e10.5_LA_16-C10


title: e10.5_AVC_15-H7


title: e10.5_LA_16-A11


title: e10.5_LA_16-A10


title: e10.5_LA_16-A1


title: e10.5_LA_16-B6


title: e10.5_LA_16-B10


title: e10.5_LA_16-B4


title: e10.5_LA_16-A6


title: e10.5_AVC_15-H4


title: e10.5_AVC_15-H11


title: e10.5_LA_16-B11


title: e10.5_AVC_15-H10


title: e10.5_LA_16-A2


title: e10.5_LA_16-A12


title: e10.5_AVC_15-G7


title: e10.5_AVC_15-H6


title: e10.5_AVC_15-H3


title: e10.5_AVC_15-H5


title: e10.5_LA_16-A4


title: e10.5_AVC_15-G2


title: e10.5_AVC_15-H2


title: e10.5_AVC_15-H12


title: e10.5_AVC_15-G11


title: e10.5_AVC_15-G9


title: e10.5_AVC_15-F8


title: e10.5_AVC_15-H1


title: e10.5_AVC_15-G10


title: e10.5_AVC_15-F7


title: e10.5_AVC_15-G12


title: e10.5_AVC_15-F5


title: e10.5_LA_16-A3


title: e10.5_AVC_15-G3


title: e10.5_AVC_15-G5


title: e10.5_AVC_15-G6


title: e10.5_AVC_15-E7


title: e10.5_AVC_15-F4


title: e10.5_AVC_15-G1


title: e10.5_AVC_15-E3


title: e10.5_AVC_15-F10


title: e10.5_AVC_15-F6


title: e10.5_AVC_15-E8


title: e10.5_AVC_15-E1


title: e10.5_AVC_15-E11


title: e10.5_AVC_15-E12


title: e10.5_AVC_15-E6


title: e10.5_AVC_15-E5


title: e10.5_AVC_15-D8


title: e10.5_AVC_15-E4


title: e10.5_AVC_15-F12


title: e10.5_AVC_15-F3


title: e10.5_AVC_15-F11


title: e10.5_AVC_15-E2


title: e10.5_AVC_15-D9


title: e10.5_AVC_15-E9


title: e10.5_AVC_15-D7


title: e10.5_AVC_15-D12


title: e10.5_AVC_15-D4


title: e10.5_AVC_15-D6


title: e10.5_AVC_15-D5


title: e10.5_AVC_15-D3


title: e10.5_AVC_15-D1


title: e10.5_AVC_15-D10


title: e10.5_AVC_15-C8


title: e10.5_AVC_15-C5


title: e10.5_AVC_15-D2


title: e10.5_AVC_15-C2


title: e10.5_AVC_15-B8


title: e10.5_AVC_15-C1


title: e10.5_AVC_15-C7


title: e10.5_AVC_15-B7


title: e10.5_AVC_15-C11


title: e10.5_AVC_15-C10


title: e10.5_AVC_15-B9


title: e10.5_AVC_15-B5


title: e10.5_AVC_15-B3


title: e10.5_AVC_15-B4


title: e10.5_AVC_15-B11


title: e10.5_AVC_15-B2


title: e10.5_AVC_15-B1


title: e10.5_AVC_15-C3


title: e10.5_AVC_15-A9


title: e10.5_AVC_15-C4


title: e10.5_AVC_15-B10


title: e10.5_AVC_15-A6


title: e10.5_AVC_15-A7


title: siDouble_Rep2_RNASeq

city: Boston

first: Pin-Ji

last: Lei

state: Massachusetts

address: 100 Blossom Street

department: Department of Radiation Oncology

institute: Massachusetts General Hospital

data_processing:
FastQC for RNASeq and ChIP-Seq raw sequence data quality control.
Cutadapt was used to remove low quality reads and adaptor contamination.
After quality control and data filter, clean reads were aligned to reference genome hg19 by TopHat v2.0.10 with at most 2 mismatch for each reads and only concordant paired end reads were accepted for downstream analysis.HTSeq-count was used for calculating reads counts for each gene with union mode and DEseq for differential expression genes analysis.
MATS.3.0.8 beta and rmats2sashimiplot were used for alternative splicing analysis and related significant splicing events plot. Splicing events were accpeted as significant with FDR < 0.05. Functional analysis and gene ontology analysis for related genes were performed by DAVID. GOplot R package was used for GO analysis plot and R version 3.2.2 was used for some custom analysis.
For ChIP-Seq data analysis, quality control was performed with FastQC, then CutAdapt was used to trim low quality bases and adaptor contamination. Bowtie was used for data mapping to hg19 reference genome and 2 mismatch was allowed at most. Samtools was used to remove duplicated reads and only unique mapped reads were allowed for next step analysis. ChIP-Seq peaks calling was performed by MACS with 1e-6 p value. The line plot of ChIP-Seq enrichment was performed by ngs.plot.
Genome_build: hg19
Supplementary_files_format_and_content: txt format file for RNA-Seq processed file
Supplementary_files_format_and_content: wig format file for ChIP-Seq processed file

organism: Homo sapiens

source_name: SPOP and SETD2 double knock down

growth_protocol: HEK293 cell was grown in DMEM(Invitrogen) supplemented with 10% fetal bovine serum and 1x penicillin/streptomycin(HyClone) at 37°C with %5 CO2.

extract_protocol:
Total RNA extractions from NC, siSPOP, siSETD2, SPOP and SETD2 double knockdown cell lines was used for RNA-Seq library construction. We mixed three independent RNA extractions for each sample in order to minimize bias. 10ug total RNA was used as initiation, and mRNA was purified by oligo-dT magnetic beads and fragmented into 200-400bp by NEBNext RNA processing kit(NEB E7525,E7490,E6111), and then reverse transcript to dscDNA.
We used Rubicon ThruPLEX DNA-seq(Rubicon #R400406) for library construction.For RNASeq library construction, about 100ng total fragmented dscDNA as initiation for end repair,adaptor ligation and PCR amplification with 15 cycles.After amplification gel selection was used to purify RNASeq library.

treatment_protocol: siRNA specificly target for SPOP and SETD2 had been used to konck-down those genes.

tag: cell line

value: embryonic kidney


title: e10.5_AVC_15-C12


title: siSPOP_Rep2_RNASeq

source_name: SPOP knock down


title: e10.5_AVC_15-A8


title: e10.5_AVC_15-B12


title: e10.5_AVC_15-A3


title: e10.5_AVC_15-B6


title: NC_Rep2_RNASeq

source_name: negative control


title: e10.5_AVC_15-A2


title: e10.5_AVC_15-A12


title: abc_10DAG_2

city: Sevilla

first: Myriam

last: Calonje

address: Americo Vespucio 49

country: Spain

institute: Institute for Plant Biochemistry and Photosynthesis (CSIC -  University of Sevilla)

data_processing:
Quality control  was performed using the software packges FastQC (http://www.bioinformatics.babraham.ac.uk/projects/fastqc/)
Read mapping to the reference genome was performed using Tophat (https://ccb.jhu.edu/software/tophat/index.shtml)
Transcript assembly was performed using Cufflinks and Cuffmerge (http://cole-trapnell-lab.github.io/cufflinks/)
Gene expression level measured in FPKM (fragments per kilobase of exon and million of mapped reads) was perfomed using Cufflinks and Cuffdiff
Subsequent data analysis and visualization was performed using the R bioconductor package cummeRbund (http://bioconductor.org/packages/release/bioc/html/cummeRbund.html)
Genome_build: tair10
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each sample

molecule: total RNA

organism: Arabidopsis thaliana

source_name: Seedlings

extract_protocol:
RNA extraction was performed using Qiagen-RNAesy mini-kit, following the manufacturer’s instruction.
RNA libraries were prepared for sequencing using standard Illumina protocols

tag: ecotype

tag: genotype

value: atbmi1abc triple mutant

tag: age


title: abc_10DAG_1


title: e10.5_AVC_15-A11


title: e10.5_AVC_15-A4


title: ab_strong_10DAG_2

value: atbmi1ab strong double mutant


title: ab_strong_10DAG_1


title: e10.5_AVC_15-A1


title: e10.5_AVC_15-A10


title: b_10DAG_2

value: atbmi1b single mutant


title: b_10DAG_1


title: e10.5_RS_13-H8

value: right ventricular septum


title: siSETD2_Rep2_RNASeq

source_name: SETD2 knock down


title: e10.5_AVC_15-A5


title: a_10DAG_2

value: atbmi1a single mutant


title: e10.5_RS_13-H6


title: e10.5_RS_13-H5


title: e10.5_RS_13-H4


title: WT_10DAG_2

value: Wild Type


title: a_10DAG_1


title: siDouble_Rep2_H3K36me3

library_source: genomic

molecule: genomic DNA

source_name: SPOP and SETD2 double knock down H3K36me3 ChIP-Seq

extract_protocol:
For ChIP assay approximately 1*10e7 cells were fixed with 1% formaldehyde and quenched by glysine.  The cells were washed three times with PBS and then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH 8.0, 1% SDS, 5 mM EDTA).  DNA was sonicated to 400~600bp before extensive centrifugation.  Four volume of ChIP dilution buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100) was added to the supernatant.  The resulted lysate was then incubated with protein G beads and antibodies at 4ºC over night.  The beads were washed 5 times and DNA was eluted by Chip elution buffer (0.1 M NaHCO3, 1% SDS, 30 ug/ml proteinase K).  The elution was incubated at 65ºC over night and DNA was extracted with DNA purification kit (Sangon).
We used Rubicon ThruPLEX DNA-seq (Rubicon #R400406) for library construction. 20-30ng H3K36me3 ChIP enriched DNA as initiation and library amplification with 15 cycles. 200-400bp DNA fragment was used for subsequent sequencing.


title: siSPOP_Rep2_H3K36me3

source_name: SPOP knock down H3K36me3 ChIP-Seq


title: e10.5_RS_13-H3


title: e10.5_RS_13-H12


title: e10.5_RS_13-H2


title: e10.5_RS_13-H1


title: siSETD2_Rep2_H3K36me3

source_name: SETD2 knock down H3K36me3 ChIP-Seq


title: Input_Rep2_H3K36me3

source_name: H3K36me3 ChIP Input


title: e10.5_RS_13-H10


title: NC_Rep2_H3K36me3

source_name: negative control H3K36me3 ChIP-Seq


title: e10.5_RS_13-G9


title: e10.5_RS_13-G6


title: e10.5_RS_13-G4


title: e10.5_RS_13-G7


title: e10.5_RS_13-G3


title: e10.5_RS_13-G2


title: e10.5_RS_13-G12


title: BAC library K562 solvent control PCRrep2

city: Amsterdam

first: Bas

last: van Steensel

address: Plesmanlaan 121

department: division of Molecular Biology

country: Netherlands

institute: Netherlands Cancer Institute

description:
Solvent_control_K562_BAC_SuRE34_PCRrep2
quantification of barcodes in cDNA
SuRE-counts-BAC.txt.gz
sure34.plasmid.norm.hemin_solvent_control_minus.160704.bw
sure34.plasmid.norm.hemin_solvent_control_plus.160704.bw

data_processing:
Basecalling and filtering were performed using standard software of the Illumina HiSeq 2000, Illumina HiSeq 2500, or Illumina MiSeq.
For plasmid library and cDNA samples the barcode sequence was extracted from the read  (using cutadapt and custom scripts) and counted.
For iPCR samples the barcode and the gDNA was extracted from the paired-end reads (using cutadapt and custom scripts). gDNA was then aligned to the reference genome (using bowtie2). Barcodes were then associated with genomic regions.
All replicate samples were pooled.
For each region identified in the iPCR data a normalized SuRE expression value was calculated as the ratio of the total readcount in the cDNA samples and the total readcount in the plasmid library samples plus a pseudocount of 1.
Genome_build: hg19
Supplementary_files_format_and_content: .txt and .wig files were generated using custom R-scripts

source_name: cell line K562

growth_protocol: K562 (ATCC® CCL-243™) were cultured according to supplier's protocol.

extract_protocol: RNA was isolated using Trisure (#BIO-38032; Bioline) and polyA RNA was purified using Oligotex from Qiagen (#70022; Qiagen). PolyA RNA was divided into 10 µl reactions containing 500 ng RNA and DNase treated with  10 units for 30 minutes (#04716728001; Roche) and DNase was inactivated by addition of 1µl 25mM EDTA and incubation at 70ºC for 10 minutes. Next, cDNA was produced by first adding 1 µl of 10 mM gene specific primer targeting the GFP ORF (247JvA) and 1 µl dNTP (10mM each) and incubating for 5 minutes at 65°C. Then 4 µl of RT buffer, 20 units RNase inhibitor (#EO0381; ThermoFisher Scientific), 200 units of  Maxima reverse transcriptase (#EP0743; ThermoFisher Scientific)  and 2.5 µl water was added and the reactionmix was incubated for 30 minutes at 50°C followed by heat-inactivation at 85° for 5 minutes. Per biological replicate, 20-30 reactions were done in parallel. Each 20 µl reaction was then PCR amplified (1’ 96 °C, 20x(15’’ 96 °C, 15’’ 60 °C, 15’’ 72 °C)) in a 100 µl reaction with MyTaq™ Red Mix and primers 151AR (containing the S1 and p5 adapter) and (index variants of) 211JvA (containing the S2, index and p7 adapter) for 21 cycles. Reactions were then pooled and 500 µl was purified using a PCR purification kit (#BIO-52060) and then size-selected using e-gel (#G6400EU; Invitrogen) and subjected to single read 50 bp high throughput sequencing on an Illumina HiSeq2000 or HiSeq2500.

treatment_protocol: 3 biological replicates (i.e. transfection replicates) were done separately of 50 million cells and harvested after 24 hours. Cells were transiently transfected using Amaxa Nucleofector II, program T-016 and nucleofection buffer as published previously (Hendel, A. et al,  Cell Rep 7, 293-305, 2014).

value: erythroleukemia


title: e10.5_RS_13-G10


title: SuRE K562 cDNA, Biorep2, PCRrep2

description:
RNA_55_K562_SuRE23_BioRep2_PCRrep2
quantification of barcodes in cDNA
SuRE-counts-K562.txt.gz
sure23.plasmid.norm.combined.45.55.minus.160504.bw
sure23.plasmid.norm.combined.45.55.plus.160504.bw


title: BAC library K562 hemin control PCRrep2

description:
Hemin_K562_BAC_SuRE34_PCRrep2
quantification of barcodes in cDNA
SuRE-counts-BAC.txt.gz
sure34.plasmid.norm.hemin_minus.160704.bw
sure34.plasmid.norm.hemin_plus.160704.bw


title: e10.5_RS_13-G1


title: BAC library K562 solvent control PCRrep1

description:
Solvent_control_K562_BAC_SuRE34_PCRrep1
quantification of barcodes in cDNA
SuRE-counts-BAC.txt.gz
sure34.plasmid.norm.hemin_solvent_control_minus.160704.bw
sure34.plasmid.norm.hemin_solvent_control_plus.160704.bw


title: SuRE K562 cDNA, Biorep1, PCRrep3

description:
RNA_45_K562_SuRE23_BioRep1_PCRrep3
quantification of barcodes in cDNA
SuRE-counts-K562.txt.gz
sure23.plasmid.norm.combined.45.55.minus.160504.bw
sure23.plasmid.norm.combined.45.55.plus.160504.bw


title: SuRE K562 cDNA, Biorep2, PCRrep1

description:
RNA_55_K562_SuRE23_BioRep2_PCRrep1
quantification of barcodes in cDNA
SuRE-counts-K562.txt.gz
sure23.plasmid.norm.combined.45.55.minus.160504.bw
sure23.plasmid.norm.combined.45.55.plus.160504.bw


title: e10.5_RS_13-F9


title: SuRE K562 cDNA, Biorep1, PCRrep2

description:
RNA_45_K562_SuRE23_BioRep1_PCRrep2
quantification of barcodes in cDNA
SuRE-counts-K562.txt.gz
sure23.plasmid.norm.combined.45.55.minus.160504.bw
sure23.plasmid.norm.combined.45.55.plus.160504.bw


title: SuRE K562 cDNA, Biorep1, PCRrep4

description:
RNA_45_K562_SuRE23_BioRep1_PCRrep4
quantification of barcodes in cDNA
SuRE-counts-K562.txt.gz
sure23.plasmid.norm.combined.45.55.minus.160504.bw
sure23.plasmid.norm.combined.45.55.plus.160504.bw


title: WT_10DAG_1


title: e10.5_RS_13-F8


title: BAC library K562 hemin control PCRrep1

description:
Hemin_K562_BAC_SuRE34_PCRrep1
quantification of barcodes in cDNA
SuRE-counts-BAC.txt.gz
sure34.plasmid.norm.hemin_minus.160704.bw
sure34.plasmid.norm.hemin_plus.160704.bw


title: plasmid library, PCR rep1

description:
Sce1_plDNA_SuRE23_PCRrep1
quantification of barcodes in plasmid library for normalization
SuRE-counts-K562.txt.gz
sure23.plasmid.norm.combined.45.55.minus.160504.bw
sure23.plasmid.norm.combined.45.55.plus.160504.bw

extract_protocol: To normalize SuRE expression data, we first characterized the barcode frequencies in the plasmid library. More specifically, we digested  1 µg library with I-SceI (#R0694S; NEB) in 25 µl to linearize the plasmids, then performed 2 replicate PCRs each on 2µl I-SceI digested material, using the same protocol as for the cDNA but for 8 cycles. Because of the high complexity of the library (~270 million) the aim was not to get a quantitative readout for each barcode, but rather to identify potentially over-represented barcodes and/or regions of the genome and normalize for that. The PCR material was e-gel size-selected and subjected to single-read 50 bp high throughput sequencing on an Illumina HiSeq2500 or HiSeq2000. In total we obtained ~40 million reads per PCR replicate. From these reads barcode counts were determined using cutadapt version 1.2.1 (http://journal.embnet.org/index.php/embnetjournal/article/view/200) to remove the adapter (GCTAGCTAACTATAACGGTCCTAAGGTAGCGAA) from the sequence.


title: plasmid library, PCR rep2

description:
Sce1_plDNA_SuRE23_PCRrep2
quantification of barcodes in plasmid library for normalization
SuRE-counts-K562.txt.gz
sure23.plasmid.norm.combined.45.55.minus.160504.bw
sure23.plasmid.norm.combined.45.55.plus.160504.bw


title: e10.5_RS_13-F6


title: SuRE K562 cDNA, Biorep1, PCRrep1

description:
RNA_45_K562_SuRE23_BioRep1_PCRrep1
quantification of barcodes in cDNA
SuRE-counts-K562.txt.gz
sure23.plasmid.norm.combined.45.55.minus.160504.bw
sure23.plasmid.norm.combined.45.55.plus.160504.bw


title: e10.5_RS_13-F4


title: e10.5_RS_13-F5


title: e10.5_RS_13-F7


title: e10.5_RS_13-F2


title: e10.5_RS_13-F12


title: siSETD2_H3K36me3

description: siSETD2 H3K36me3 ChIP

data_processing:
FastQC for ChIPSeq and RNASeq raw sequence data quality control.
Cutadapt was used to remove low quality reads and adaptor contamination.
RNASeq clean data mapped to hg19 reference genome by tophat2 with at most 2 mismatch per reads.
ChIPSeq clean data mapped to hg19 reference genome by bowtie with at most 2 mismatch and only unique mapped reads were allowed for next step analysis.
HT-seq and DESeq were used for differential expression genes analysis and MACS was used for ChIPSeq peaks calling.
Genome_build: hg19
Supplementary_files_format_and_content: count file for RNASeq mapped results and wig file for ChIPSeq mapped results.

extract_protocol:
10ug total RNA was used for RNASeq library construction. mRNA was purified by oligo-dT magnetic beads and fragmented into 200-400bp by NEBNext RNA processing kit(NEB E7525,E7490,E6111), and then reverse transcript to dscDNA. For ChIP assay, DNA and protein were cross-link and digested into single nucleosome by using micrococcal nuclease, and then H3K36me3(abcam #ab9050) ChIP grade antibody was used for IP enrichment. ChIP DNA was reverse cross-link at 65°C overnight and  eluted by TE buffer.
We used Rubicon ThruPLEX DNA-seq(Rubicon #R400406) for library construction.For RNASeq library construction, about 100ng total fragmented dscDNA as initiation for end repair,adaptor ligation and PCR amplification with 15 cycles.After amplification gel selection was used to purify RNASeq library. For ChIPSeq library construction, about 20ng ChIPed DNA as initiation. The PCR amplification cycles for ChIPSeq was 18 and gel selection for library purification.

tag: genotype/variation

value: SETD2 knock down

tag: antibody


title: siSPOP_H3K36me3

description: siSPOP H3K36me3 ChIP

value: SPOP knock down


title: e10.5_RS_13-F3


title: Input ChIPSeq

description: ChIP Input

value: input


title: siDouble_H3K36me3

description: siSPOP and siSETD2 H3K36me3 ChIP

value: SPOP SETD2 double knock down


title: e10.5_RS_13-F11


title: e10.5_RS_13-F10


title: siDouble_RNASeq

description: SPOP and SETD2 double knock down


title: siSETD2_RNASeq

description: SETD2 knock down


title: siSPOP_RNASeq

description: SPOP knock down


title: NC_RNASeq

description: negative control

value: wild type


title: e10.5_RS_13-F1


title: e10.5_RS_13-E8


title: e10.5_RS_13-E6


title: e10.5_RS_13-E7


title: e10.5_RS_13-E9


title: Retroviral cDNA Library (Genomic DNA from Bone Marrow Cells)

city: Seoul

first: Hee Yong

last: Chung

address: FTC Building 12th Fl. Hanengdangdong 17

department: Biomedical Science

country: South Korea

institute: Hanyang University

description: PCR amplified cDNA MIx

data_processing:
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence and masked for low-complexity or low-quality sequence with Trimmomatic0.32.
To estimate expression levels, the RNA-Seq reads were mapped to the genome of Homo sapiens using TopHat 2.0.13, which is capable of reporting split-read alignments across splice junctions and determined using Cufflinks 2.2.1 software in default options.
The reference genome sequence of Homo sapiens and annotation data were downloaded from UCSC. The transcript counts in isoform level were calculated, and the relative transcript abundances were measured in FPKM (Fragments Per Kilobase of exon per Million fragments mapped) using Cufflinks.
Raw data (the reads for each transcript) were normalized by FPKM (Fragments Per Kilobase of exon model per Millon mapped fragments).
Genome_build: hg19
Supplementary_files_format_and_content: tab-delimited text file includes FPKM values for each Sample

source_name: PCR amplified cDNA fragments

extract_protocol:
cDNAs were amplified by PCR from the plasmid mix or from the genomic DNA from the bone marrow cells transduced with the retroviral cDNA library. The amplified DNA were extracted with PCR clean up kit from Clontech Co.
The sequencing libraries were then prepared according to the manufacturer’s instructions of TruSeq Nano DNA Library Prep Kit (Illumina, #FC-121-4003). Briefly, fragmentation of 1ug of genomic DNA was performed using adaptive focused acoustic technology.(AFA; Covaris) The fragmented DNA is repaired, an ‘A’ is ligated to the 3′ end, Truseq adapters are then ligated to the fragments. Once ligation had been assessed, the adapter ligated product is PCR amplified. The final purified product is then quantified using qPCR according to the qPCR Quantification Protocol Guide and qualified using the Agilent Technologies 2200 TapeStation. (Agilent technologies). And then we sequenced using the HiSeq™ 2500 platform (Illumina)

tag: sample type

value: cDNA inserts amplified from the genomic DNA of bone marrow cells transduced with the retroviral cDNA library

tag: sample name

value: D


title: e10.5_RS_13-E2


title: e10.5_RS_13-E5


title: e10.5_RS_13-E4


title: e10.5_RS_13-E3


title: e10.5_RS_13-E11


title: e10.5_RS_13-E1


title: e10.5_RS_13-E10


title: e10.5_RS_13-D8


title: e10.5_RS_13-D9


title: NC_H3K36me3

description: negative control H3K36me3 CHIP


title: e10.5_RS_13-D6


title: e10.5_RS_13-D5


title: e10.5_RS_13-D4


title: e10.5_RS_13-D2


title: e10.5_RS_13-D3


title: e10.5_RS_13-D12


title: e10.5_RS_13-D11


title: e10.5_RS_13-D10


title: e10.5_RS_13-D1


title: Retroviral cDNA Library (Plasmid Mix)

description: PCR amplified cDNA Mix

molecule: other

value: cDNA inserts amplified from the retroviral cDNA library itself

value: A


title: e10.5_RS_13-C9


title: e10.5_RS_13-C8


title: e10.5_RS_13-C7


title: e10.5_RS_13-C6


title: e10.5_RS_13-C10


title: e10.5_RS_13-C2


title: e10.5_RS_13-C3


title: e10.5_RS_13-C12


title: e10.5_RS_13-C11


title: e10.5_RS_13-C4


title: e10.5_RS_13-B9


title: e10.5_RS_13-B3


title: e10.5_RS_13-B8


title: e10.5_RS_13-B12


title: e10.5_RS_13-B5


title: e10.5_RS_13-B6


title: e10.5_RS_13-B4


title: e10.5_RS_13-B11


title: e10.5_RS_13-A5


title: e10.5_RS_13-A7


title: e10.5_RS_13-B1


title: e10.5_RS_13-B10


title: e10.5_RS_13-A9


title: e10.5_RS_13-A12


title: e10.5_RS_13-A10


title: e10.5_RS_13-A4


title: e10.5_RS_13-A1


title: e10.5_RS_13-A3


title: e10.5_RV_12-H6

value: right ventricle


title: e10.5_RV_12-H7


title: e10.5_RV_12-H5


title: e10.5_RS_13-C1


title: e10.5_RV_12-H2


title: e10.5_RV_12-H3


title: e10.5_RS_13-B2


title: e10.5_RV_12-H11


title: e10.5_RV_12-H10


title: e10.5_RV_12-H12


title: e10.5_RV_12-H1


title: e10.5_RS_13-A8


title: e10.5_RV_12-G9


title: e10.5_RV_12-G6


title: e10.5_RV_12-G7


title: e10.5_RV_12-G11


title: e10.5_RV_12-G4


title: e10.5_RV_12-G8


title: e10.5_RV_12-G3


title: e10.5_RV_12-G10


title: e10.5_RV_12-F8


title: e10.5_RV_12-F6


title: e10.5_RV_12-F7


title: e10.5_RV_12-F9


title: e10.5_RV_12-F4


title: e10.5_RV_12-F11


title: e10.5_RV_12-F3


title: e10.5_RV_12-F10


title: e10.5_RV_12-H4


title: e10.5_RV_12-F1


title: e10.5_RV_12-E9


title: e10.5_RV_12-E5


title: e10.5_RV_12-E6


title: e10.5_RV_12-E4


title: e10.5_RV_12-E8


title: e10.5_RV_12-E7


title: e10.5_RV_12-E3


title: e10.5_RV_12-E1


title: e10.5_RV_12-D9


title: e10.5_RV_12-D7


title: e10.5_RV_12-D4


title: e10.5_RV_12-D8


title: e10.5_RV_12-D6


title: e10.5_RV_12-D11


title: e10.5_RV_12-D12


title: e10.5_RV_12-D2


title: e10.5_RV_12-D10


title: e10.5_RV_12-D3


title: e10.5_RV_12-C8


title: e10.5_RV_12-F12


title: e10.5_RV_12-C7


title: e10.5_RV_12-C4


title: e10.5_RV_12-C3


title: e10.5_RV_12-C2


title: e10.5_RV_12-C12


title: e10.5_RV_12-C11


title: e10.5_RV_12-E2


title: e10.5_RV_12-C10


title: e10.5_RV_12-B9


title: e10.5_RV_12-B8


title: e10.5_RV_12-B5


title: e10.5_RV_12-B3


title: e10.5_RV_12-B12


title: e10.5_RV_12-B6


title: e10.5_RV_12-B2


title: e10.5_RV_12-D1


title: e10.5_RV_12-C9


title: e10.5_RV_12-B1


title: e10.5_RV_12-A9


title: e10.5_RV_12-B10


title: e10.5_RV_12-A7


title: e10.5_RV_12-A4


title: e10.5_RV_12-A12


title: e10.5_RV_12-A11


title: e10.5_RV_12-A3


title: e10.5_LS_11-H2

value: left ventricular septum


title: e10.5_LS_11-H5


title: e10.5_LS_11-H7


title: e10.5_LS_11-H11


title: e10.5_RV_12-C1


title: e10.5_LS_11-G8


title: e10.5_LS_11-H1


title: e10.5_LS_11-G7


title: e10.5_LS_11-G6


title: e10.5_LS_11-G9


title: e10.5_LS_11-G4


title: e10.5_LS_11-G5


title: e10.5_LS_11-G2


title: e10.5_LS_11-G12


title: e10.5_RV_12-B11


title: e10.5_LS_11-G3


title: e10.5_LS_11-G1


title: e10.5_LS_11-F9


title: e10.5_RV_12-A1


title: e10.5_LS_11-F8


title: e10.5_LS_11-F5


title: e10.5_RV_12-A5


title: e10.5_LS_11-F7


title: e10.5_LS_11-F4


title: e10.5_LS_11-F10


title: e10.5_LS_11-F12


title: e10.5_LS_11-E9


title: e10.5_LS_11-F1


title: e10.5_LS_11-E4


title: e10.5_LS_11-E10


title: e10.5_LS_11-E8


title: e10.5_LS_11-E1


title: e10.5_LS_11-D6


title: e10.5_LS_11-D3


title: e10.5_LS_11-E5


title: e10.5_LS_11-E6


title: e10.5_LS_11-E2


title: e10.5_LS_11-C5


title: e10.5_LS_11-E7


title: e10.5_LS_11-C3


title: e10.5_LS_11-C10


title: e10.5_LS_11-B8


title: e10.5_LS_11-D7


title: e10.5_LS_11-D5


title: e10.5_LS_11-E12


title: e10.5_LS_11-E11


title: e10.5_LS_11-D4


title: e10.5_LS_11-D8


title: e10.5_LS_11-B6


title: e10.5_LS_11-B12


title: e10.5_LS_11-D12


title: e10.5_LS_11-D10


title: e10.5_LS_11-D2


title: e10.5_LS_11-D11


title: e10.5_LS_11-C7


title: e10.5_LS_11-B3


title: e10.5_LS_11-B5


title: e10.5_LS_11-B1


title: e10.5_LS_11-B11


title: e10.5_LS_11-B2


title: e10.5_LS_11-C1


title: e10.5_LS_11-C4


title: e10.5_LS_11-C11


title: e10.5_LS_11-A5


title: e10.5_LS_11-A8


title: e10.5_LS_11-A4


title: e10.5_LS_11-A7


title: e10.5_LS_11-B4


title: e10.5_LS_11-A12


title: e10.5_LS_11-A11


title: e10.5_LS_11-A10


title: e10.5_LS_11-A1


title: e9.5_LA_1-H7

source_name: e9.5 left atrium

value: left atrium


title: e10.5_LS_11-A3


title: e9.5_LA_1-H6


title: e10.5_LS_11-B10


title: e9.5_LA_1-H5


title: e10.5_LS_11-A9


title: e9.5_LA_1-H4


title: e9.5_LA_1-H11


title: e9.5_LA_1-H12


title: e9.5_LA_1-H10


title: e9.5_LA_1-G9


title: e9.5_LA_1-G4


title: e9.5_LA_1-G6


title: e9.5_LA_1-G5


title: e9.5_LA_1-H1


title: e9.5_LA_1-G3


title: e9.5_LA_1-G2


title: e9.5_LA_1-G10


title: e9.5_LA_1-F8


title: e9.5_LA_1-G1


title: e9.5_LA_1-G11


title: e9.5_LA_1-F4


title: e9.5_LA_1-F7


title: e9.5_LA_1-F6


title: e9.5_LA_1-F9


title: e9.5_LA_1-G12


title: e9.5_LA_1-H3


title: e9.5_LA_1-F12


title: e9.5_LA_1-F11


title: e9.5_LA_1-F10


title: e9.5_LA_1-G7


title: e9.5_LA_1-G8


title: e9.5_LA_1-E7


title: e9.5_LA_1-E4


title: e9.5_LA_1-E6


title: e9.5_LA_1-E3


title: e9.5_LA_1-F1


title: e9.5_LA_1-E10


title: e9.5_LA_1-E1


title: e9.5_LA_1-H2


title: e9.5_LA_1-E5


title: e9.5_LA_1-E8


title: e10.5_LS_11-B7


title: e9.5_LA_1-D8


title: e9.5_LA_1-D9