title: Single-cell_RNA-seq_Subject1_TEMRA_Cell005


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell187

status: Public on Jan 24 2018

type: SRA

city: La Jolla

first: Pandurangan

last: Vijayanand

state: California

address: 9420 Athena Circle

department: Division of Vaccine Discovery

country: USA

institute: La Jolla Institute for Allergy and Immunology

description: Whole Transcriptome Amplification (SmartSeq2 - Picelli et al.2014)

library_source: transcriptomic

data_processing:
Single-cell RNA–seq data were mapped against the human hg19 reference genome using TopHat (v1.4.1., --library-type fr-secondstrand -C) and Gencode version 19 (GRCh37.p13) as gene model reference for alignment.
Sequencing read coverage per gene was counted using HTSeq-count (-m union -s yes -t exon -i gene_id, http://www huber.embl.de/users/anders/HTSeq/). Counts per gene are obtained by counting all the gene_id, http://www huber.embl.de/users/anders/HTSeq/). Counts per gene are obtained by counting all the transcripts mapping to a gene and is together referred to as transcript.
Genome_build: hg19
Supplementary_files_format_and_content: SC_raw_counts.txt contains raw counts of sequencing reads obtained with HTSeq-count

molecule: total RNA

organism: Homo sapiens

growth_protocol: CD4 memory cell types were isolated from PBMCs and directly sorted by Flow cytometry into a 96-well plate wells with lysis buffer with RNAase inhibitor (Takara) - Described in Picelli et al. Nat Protoc. 2014, PMID:24385147. No particular cell growth procedure was required.

extract_protocol:
Single cell RNAseq was performed as described in Picelli et al.Nat Protoc. 2014, PMID:24385147. We performed 23 cycles of amplification.
Single cell RNAseq was perfromed as described in Picelli et al.Nat Protoc. 2014, PMID:24385147. Barcoded Illumina sequencing libraries (Nextera XT library preparation kit, Illumina) were prepared as following manufacturer's protocol.
DNA sequencing - Libraries were sequenced on the Illumina HiSeq 2500  platform to obtain 50-bp single end reads (barcoded Illumina sequencing libraries (Nextera XT library preparation kit, Illumina)).

treatment_protocol: For DENV antigen specifc cells, PBMCs were stimulated with DENV peptide pool following the protocol described previously by Weiskopf et al., 2014 and IFNγ producing single cells were sorted for single-cell RNA-seq analysis

tag: subject id

tag: dengue infections history

value: DENV negative

tag: cell type

value: PBMC

tag: cell subtype surface markers

tag: cell subtype


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell183


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell184


title: Single-cell_RNA-seq_Subject1_TEMRA_Cell002

source_name: Subject1_DENV negative_PBMC_TEMRA

value: TEMRA


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell181


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell182


title: Single-cell_RNA-seq_Subject1_TEMRA_Cell019


title: Single-cell_RNA-seq_Subject1_TEMRA_Cell010


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell180


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell179


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell185


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell177


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell176


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell175


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell167


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell169


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell165


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell172


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell173


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell166


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell171


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell178


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell164


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell170


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell163


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell159


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell161


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell160


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell156


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell162


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell168


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell157


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell153


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell154


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell158


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell155


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell150


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell174


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell152


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell151


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell147


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell146


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell148


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell149


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell145


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell143


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell144


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell140


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell142


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell141


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell139


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell131


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell138


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell136


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell129


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell133


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell128


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell130


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell135


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell123


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell132


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title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell134


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title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell117


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title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell137


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell115


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell124


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell113


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell112


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell114


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell110


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell109


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell106


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell108


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell103


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell122


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell107


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell096


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title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell097


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell099


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell105


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell100


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title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell094


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell089


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell111


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell088


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell084


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell085


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell087


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell102


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell086


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell083


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell081


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell080


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell079


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell082


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell078


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title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell068


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell071


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell070


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell075


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell073


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell074


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell063


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell062


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell061


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell065


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell069


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell064


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell060


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell066


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title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell056


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell059


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell057


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell077


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell055


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title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell067


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell051


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title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell054


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell050


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell052


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell053


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell045


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell048


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell047


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell044


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell040


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell037


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell041


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell038


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell034


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell039


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell033


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell032


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell031


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell043


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell025


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title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell026


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell030


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell027


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell020


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell042


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell019


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell018


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell017


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell036


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell016


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell015


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell035


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell013


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell014


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell023


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell024


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell011


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell008


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell009


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell010


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell007


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell022


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell012


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell006


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell005


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell003


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell001


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell004


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell187


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell188


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell184


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell002


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell185


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell021


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell180


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell172


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell182


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell181


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell171


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title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell178


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell174


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title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell175


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell166


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell179


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell169


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell167


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell183


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell173


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell163


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell170


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell162


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell159


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell164


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title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell077


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell074


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell095


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell070


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell069


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell071


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell068


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell067


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell066


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell075


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell063


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell062


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell064


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell061


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell060


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell058


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell057


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell054


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell053


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell051


title: Single-cell_RNA-seq_Subject16_IL7Rlow_TEMRA_Cell029


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell056


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell059


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell049


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell050


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell055


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell072


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell047


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell048


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell046


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell045


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell065


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell042


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell043


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell044


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell039


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell040


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell041


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell038


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell052


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell037


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell035


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell036


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell073


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell034


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell032


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell031


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell029


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell027


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell030


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell033


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell026


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell028


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell024


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell023


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell025


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell022


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell020


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell021


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell019


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell018


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell015


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell017


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell016


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell013


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell011


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell006


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell008


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell010


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell009


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell005


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell012


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell004


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell003


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell091

source_name: Subject15_DENV positive_PBMC_TEMRA

value: DENV positive


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell088


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell087


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell086


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell002


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell092


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell085


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell083


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell089


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell090


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell014


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell081


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell080


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell082


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell007


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell077


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell001


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell078


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell079


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell071


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell073


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell072


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell070


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell067


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell075


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell068


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell066


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell063


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell065


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell084


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell064


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell061


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell060


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell062


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell058


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell057


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell059


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell054


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell055


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell074


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell056


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell053


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell069


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell052


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell051


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell050


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell038


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell037


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell036


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell046


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell045


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell040


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell035


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell043


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell042


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell047


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell041


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell049


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell048


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell076


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell029


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell030


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell032


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell033


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell044


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell026


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell025


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell027


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell028


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell034


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell023


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell024


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell031


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell017


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell014


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell022


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell013


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell012


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell015


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell008


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell019


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell007


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell021


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell005


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell004


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell003


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell001


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell002


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell071

source_name: Subject15_DENV positive_PBMC_DENV antigen specific TEMRA

value: DENV antigen specific TEMRA


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell072


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell011


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell010


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell016


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell018


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell020


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell070


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell069


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell068


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell067


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell006


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell066


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell039


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell061


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell057


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell062


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell065


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell058


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell059


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell060


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell064


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell056


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell063


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell040


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell049


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell044


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell047


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell041


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell054


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell052


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell043


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell045


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell042


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell050


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell053


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell055


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell051


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell048


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell046


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell039


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell038


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell036


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell034


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell035


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell037


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell032


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell026


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell028


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell030


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell024


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell025


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell021


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell023


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell029


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell027


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell019


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell022


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell020


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell014


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell017


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell018


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell013


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell011


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell016


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell012


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell010


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell033


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell031


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell009


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell008


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell007


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell006


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell005


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell015


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell001


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell002


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell094

source_name: Subject14_DENV positive_PBMC_TEMRA


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell088


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell092


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell091


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell093


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell089


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell090


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell086


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell084


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell083


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell087


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell085


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell080


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell081


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell082


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell078


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell079


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell076


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell077


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell075


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell074


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell004


title: Single-cell_RNA-seq_Subject15_DENV_TEMRA_Cell003


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell071


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell072


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell070


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell064


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell066


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell067


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell068


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell069


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell057


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell060


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell062


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell063


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell053


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell058


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell054


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell061


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell059


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell056


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell055


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell051


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell073


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell052


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell050


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell049


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell047


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell048


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell046


title: Single-cell_RNA-seq_Subject15_TEMRA-Cell009


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell045


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell043


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell041


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell042


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell040


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell039


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell037


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell034


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell032


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell033


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell028


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell030


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell029


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell031


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell035


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell027


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell023


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell025


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell044


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell024


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell020


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell021


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell019


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell018


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell038


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell026


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell017


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell036


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell014


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell013


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell015


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell012


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell010


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell065


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell022


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell006


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell005


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell004


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell007


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell008


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell009


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell057

source_name: Subject14_DENV positive_PBMC_DENV antigen specific TEMRA


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell002


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell001


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell056


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell058


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell003


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell011


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell050


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell053


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell049


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell052


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell051


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell045


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell046


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell048


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell047


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell054


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell044


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell042


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell043


title: Single-cell_RNA-seq_Subject14_TEMRA-Cell016


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell055


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell041


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell037


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell035


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell039


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell038


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell036


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell040


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell030


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell029


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell034


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell032


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell033


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell027


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell028


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell026


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell023


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell022


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell018


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell025


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell024


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell021


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell015


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell016


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell017


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell014


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell020


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell012


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell031


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell013


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell011


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell006


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell008


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell007


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell009


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell019


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell005


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell001


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell004


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell003


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell093

source_name: Subject13_DENV positive_PBMC_TEMRA


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell091


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell088


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell092


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell090


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell085


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell089


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell086


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell087


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell084


title: Single-cell_RNA-seq_Subject16_IL7Rhigh_TEMRA_Cell128


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell083


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell082


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell081


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell080


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell079


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell078


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell010


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell094


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell073


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell072


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell077


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell074


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell075


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell071


title: Single-cell_RNA-seq_Subject14_DENV_TEMRA_Cell002


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell066


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell068


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell065


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell064


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell067


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell069


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell061


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell062


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell057


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell059


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell076


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell055


title: norm_24h_n1_1

city: Frankfurt am Main

first: Philipp

last: Neumann

state: Hessen

address: Theodor-Stern-Kai 7

country: Germany

institute: Institute for Cardiovascular Regeneration

description: Normoxic conditions

data_processing:
Reads were mapped with Tophat default parameters to GRCH37. Afterwards, FPKM values where determined by Cuffdiff.
Genome_build: GRCH37
Supplementary_files_format_and_content: Tab delimited text file wich includes FPKM values for each sample

source_name: Human umbilical vein endothelial cells

growth_protocol: Pooled human umbilical vein endothelial cells (Lonza) were cultured as recommended by the manufacturer. Cells were kept at 5% CO2, 20% O2, 37°C and humidified atmosphere. Endothelial cell were cultured in endothelial basal medium containing EGM SingleQuots (Lonza) and 10% fetal calf serum (Invitrogen).

extract_protocol:
RNA deep sequencing was performed by analyzing ribosomal depleted total RNA from human umbilical vein endothelial cells. RNA was isolated using a RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions including DNA digestion.
RNA was fragmented and primed for cDNA synthesis. Libraries were created using a NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina as recommended by the manufacturer.

treatment_protocol: Hypoxia (0.2% O2) was induced in a humidified atmosphere. Hypoxic pO2 levels were verified by measuring VEGFA induction by RT-qPCR and pO2 levels with a hypoxia sensing probe from Oxford Optronix (Oxford, UK). RNA was isolated and processed for RNA deep sequencing.

value: Endothelial cells

tag: passage

value: Passage 2

tag: treatment

value: Normoxia 20% O2, 5% CO2 humidified atmosphere


title: norm_24h_n2_1

description: Normoxic conditions


title: hyp_24h_n2_1

description: Hypoxic conditions

value: Hypoxia 0.2% O2, 5% CO2 humidified atmosphere


title: norm_12h_n1_1

description: Normoxic conditions


title: norm_12h_n2_1

description: Normoxic conditions


title: hyp_12h_n2_1

description: Hypoxic conditions


title: hyp_12h_n1_1

description: Hypoxic conditions


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell070


title: hyp_24h_n1_1

description: Hypoxic conditions


title: Single-cell_RNA-seq_Subject2_TEMRA

description: 3' Transcriptome sequencing (10X genomics)

data_processing:
Reads from single-cell RNA-seq were aligned and collapsed into unique molecular identifier (UMI) counts using 10x genomics’s cell-ranger software (version 1.2)
Counts were normalized to get UMIs per million reads (UPM)
Genome_build: hg19

growth_protocol: CD4 memory cell types were isolated from PBMCs and directly sorted by Flow cytometry into 50% FBS. No particular cell growth procedure was required.

extract_protocol:
Single-cell RNA-seq using 10X genomics platform was performed using Chromium™ Single Cell 3' v2 Reagent Kits following the manufacturer’s protocol (Zheng et. al., 2017).
Libraries were constructed using Chromium™ Single Cell 3' v2 Reagent Kits following the manufacturer’s protocol
Libraries were sequenced on HiSeq2500 platform to obtain 100 and 32-bp paired end reads using the following read length; read 1, 26 cycles, read 2, 98 cycles and i7 index, 8 cycles.

treatment_protocol: No particular treatment was done to the PBMC. PBMCs were immuno stained and FACS sorted into 50% FBS and processed for single-cell RNA-seq using 10X genomics.

tag: subject #

tag: subject status


title: Single-cell_RNA-seq_Subject1_TEMRA


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell063


title: Bulk_RNA-seq_Subject23_V2_TEM

description: Whole Transcriptome Amplification (Smart-seq2 - Picelli et al.2014)

data_processing:
Bulk RNA–seq data were mapped against the human hg19 reference genome using TopHat (v1.4.1., --library-type fr-secondstrand -C) and Gencode version 19 (GRCh37.p13) as gene model reference for alignment.
Sequencing read coverage per gene was counted using HTSeq-count (-m union -s yes -t exon -i gene_id, http://www huber.embl.de/users/anders/HTSeq/). Counts per gene are obtained by counting all the gene_id, http://www huber.embl.de/users/anders/HTSeq/). Counts per gene are obtained by counting all the transcripts mapping to a gene and is together referred to as transcript.
Genome_build: hg19
Supplementary_files_format_and_content: Bulk_raw_counts.txt contains raw counts of sequencing reads obtained with HTSeq-count

molecule: polyA RNA

source_name: Subject23_Visit 2_PBMC_TEM

growth_protocol: CD4 memory cell types were isolated from PBMCs and directly sorted by Flow cytometry into trizol. No particular cell growth procedure was required.

extract_protocol:
RNA-seq was performed as described previously by Seumois et al 2016 and Ganesan et al 2017 using smart-seq2 method  with 0.5ng of total RNA. Total RNA was purified using a miRNAeasy kit (Qiagen) and quantified as described previously (Seumois et al.Am J Clin Exp Immunol. 2012; PMID:23304658).
Barcoded Illumina sequencing libraries were sonstructed as per the protocol described by illumina (Nextera XT library preparation kit, Illumina).
DNA sequencing - Libraries were sequenced on the HiSeq2500 Illumina platform to obtain 50-bp single end reads (barcoded Illumina sequencing libraries (Nextera XT library preparation kit, Illumina)).

treatment_protocol: No particular treatment was done to the PBMC. PBMCs were immuno stained and FACS sorted into Trizol for RNA isolation

tag: longitudinal visit

value: Visit 2

value: TEM


title: Bulk_RNA-seq_Subject23_V2_TCM

source_name: Subject23_Visit 2_PBMC_TCM

value: TCM


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell060


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell058


title: Bulk_RNA-seq_Subject23_V1_TEM

source_name: Subject23_Visit 1_PBMC_TEM

value: Visit 1


title: Bulk_RNA-seq_Subject23_V2_IL7Rhigh_TEMRA

source_name: Subject23_Visit 2_PBMC_IL7Rhigh_TEMRA


title: Bulk_RNA-seq_Subject23_V2_IL7Rlow_TEMRA

source_name: Subject23_Visit 2_PBMC_IL7Rlow_TEMRA


title: Single-cell_RNA-seq_Subject13_TEMRA-Cell056


title: Bulk_RNA-seq_Subject23_V1_IL7Rlow_TEMRA

source_name: Subject23_Visit 1_PBMC_IL7Rlow_TEMRA


title: Bulk_RNA-seq_Subject22_V2_TEM

source_name: Subject22_Visit 2_PBMC_TEM


title: Bulk_RNA-seq_Subject22_V2_TCM

source_name: Subject22_Visit 2_PBMC_TCM


title: Bulk_RNA-seq_Subject23_V1_IL7Rhigh_TEMRA

source_name: Subject23_Visit 1_PBMC_IL7Rhigh_TEMRA


title: Bulk_RNA-seq_Subject23_V1_TCM

source_name: Subject23_Visit 1_PBMC_TCM


title: Bulk_RNA-seq_Subject22_V2_IL7Rhigh_TEMRA

source_name: Subject22_Visit 2_PBMC_IL7Rhigh_TEMRA


title: Bulk_RNA-seq_Subject22_V1_TCM

source_name: Subject22_Visit 1_PBMC_TCM


title: Bulk_RNA-seq_Subject22_V1_IL7Rhigh_TEMRA

source_name: Subject22_Visit 1_PBMC_IL7Rhigh_TEMRA


title: Bulk_RNA-seq_Subject21_V2_TCM

source_name: Subject21_Visit 2_PBMC_TCM


title: Bulk_RNA-seq_Subject21_V2_TEM

source_name: Subject21_Visit 2_PBMC_TEM


title: Bulk_RNA-seq_Subject21_V2_IL7Rlow_TEMRA

source_name: Subject21_Visit 2_PBMC_IL7Rlow_TEMRA


title: Bulk_RNA-seq_Subject21_V2_IL7Rhigh_TEMRA

source_name: Subject21_Visit 2_PBMC_IL7Rhigh_TEMRA


title: Bulk_RNA-seq_Subject21_V1_IL7Rlow_TEMRA

source_name: Subject21_Visit 1_PBMC_IL7Rlow_TEMRA


title: Bulk_RNA-seq_Subject20_V2_TEM

source_name: Subject20_Visit 2_PBMC_TEM


title: Bulk_RNA-seq_Subject21_V1_TEM

source_name: Subject21_Visit 1_PBMC_TEM


title: Bulk_RNA-seq_Subject20_V2_IL7Rlow_TEMRA

source_name: Subject20_Visit 2_PBMC_IL7Rlow_TEMRA


title: Bulk_RNA-seq_Subject20_V1_IL7Rlow_TEMRA

source_name: Subject20_Visit 1_PBMC_IL7Rlow_TEMRA


title: Bulk_RNA-seq_Subject20_V1_TCM

source_name: Subject20_Visit 1_PBMC_TCM


title: Bulk_RNA-seq_Subject20_V2_IL7Rhigh_TEMRA

source_name: Subject20_Visit 2_PBMC_IL7Rhigh_TEMRA


title: Bulk_RNA-seq_Subject16_V2_TEM

source_name: Subject16_Visit 2_PBMC_TEM


title: Bulk_RNA-seq_Subject21_V1_TCM

source_name: Subject21_Visit 1_PBMC_TCM


title: Bulk_RNA-seq_Subject16_V1_TCM

source_name: Subject16_Visit 1_PBMC_TCM


title: Bulk_RNA-seq_Subject20_V2_TCM

source_name: Subject20_Visit 2_PBMC_TCM


title: Bulk_RNA-seq_Subject16_V2_IL7Rhigh_TEMRA

source_name: Subject16_Visit 2_PBMC_IL7Rhigh_TEMRA


title: Bulk_RNA-seq_Subject16_V2_TCM

source_name: Subject16_Visit 2_PBMC_TCM


title: Bulk_RNA-seq_Subject20_V1_TEM

source_name: Subject20_Visit 1_PBMC_TEM


title: Bulk_RNA-seq_Subject16_V1_IL7Rhigh_TEMRA

source_name: Subject16_Visit 1_PBMC_IL7Rhigh_TEMRA


title: Bulk_RNA-seq_Subject20_V1_IL7Rhigh_TEMRA

source_name: Subject20_Visit 1_PBMC_IL7Rhigh_TEMRA


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell044

source_name: Subject9_DENV negative_PBMC_TEMRA


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell047


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell043


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell045


title: Bulk_RNA-seq_Subject22_V1_IL7Rlow_TEMRA

source_name: Subject22_Visit 1_PBMC_IL7Rlow_TEMRA


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell041


title: Bulk_RNA-seq_Subject16_V1_IL7Rlow_TEMRA

source_name: Subject16_Visit 1_PBMC_IL7Rlow_TEMRA


title: Bulk_RNA-seq_Subject22_V1_TEM

source_name: Subject22_Visit 1_PBMC_TEM


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell042


title: Bulk_RNA-seq_Subject21_V1_IL7Rhigh_TEMRA

source_name: Subject21_Visit 1_PBMC_IL7Rhigh_TEMRA


title: Bulk_RNA-seq_Subject16_V2_IL7Rlow_TEMRA

source_name: Subject16_Visit 2_PBMC_IL7Rlow_TEMRA


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell040


title: Bulk_RNA-seq_Subject16_V1_TEM

source_name: Subject16_Visit 1_PBMC_TEM


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell035


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell036


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell037


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell031


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell033


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell032


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell030


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell029


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell025


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell027


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell038


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell028


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell026


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell023


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell020


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell019


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell018


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell024


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell021


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell022


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell016


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell014


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell013


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell034


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell017


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell011


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell039


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell012


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell015


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell008


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell010


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell009


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell007


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell006


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell004


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell002


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell001


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell003


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell048

source_name: Subject8_DENV positive_PBMC_TEMRA


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell045


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell043


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell042


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell044


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell046


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell041


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell040


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell039


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell038


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell035


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell005


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell036


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell037


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell034


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell033


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell031


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell047


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell032


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell029


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell028


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell027


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell026


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell024


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell023


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell022


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell020


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell019


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell018


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell017


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell016


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell015


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell011


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell013


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell014


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell012


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell030


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell021


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell025


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell006


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell007


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell009


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell010


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell005


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell047

source_name: Subject7_DENV negative_PBMC_TEMRA


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell048


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell002


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell001


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell003


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell008


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell043


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell041


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell044


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell042


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell045


title: Single-cell_RNA-seq_Subject8_TEMRA_Cell004


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell040


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell039


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell035


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell034


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell033


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell037


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell038


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell036


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell046


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell032


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell031


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell028


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell027


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell026


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell030


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell025


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell021


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell020


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell029


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell023


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell016


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell015


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell018


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell022


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell019


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell014


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell012


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell013


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell009


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell010


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell006


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell004


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell005


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell024


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell017


title: Single-cell_RNA-seq_Subject6_TEM_Cell048

source_name: Subject6_DENV positive_PBMC_TEM


title: Single-cell_RNA-seq_Subject9_TEMRA_Cell046


title: Single-cell_RNA-seq_Subject6_TEM_Cell047


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell002


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell001


title: Single-cell_RNA-seq_Subject6_TEM_Cell043


title: Single-cell_RNA-seq_Subject6_TEM_Cell042


title: Single-cell_RNA-seq_Subject6_TEM_Cell044


title: Single-cell_RNA-seq_Subject6_TEM_Cell046


title: Single-cell_RNA-seq_Subject6_TEM_Cell040


title: Single-cell_RNA-seq_Subject6_TEM_Cell038


title: Single-cell_RNA-seq_Subject6_TEM_Cell037


title: Single-cell_RNA-seq_Subject6_TEM_Cell039


title: Bulk_RNA-seq_Subject22_V2_IL7Rlow_TEMRA

source_name: Subject22_Visit 2_PBMC_IL7Rlow_TEMRA


title: Single-cell_RNA-seq_Subject6_TEM_Cell045


title: Single-cell_RNA-seq_Subject6_TEM_Cell036


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell008


title: Single-cell_RNA-seq_Subject6_TEM_Cell034


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell003


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell011


title: Single-cell_RNA-seq_Subject7_TEMRA_Cell007


title: Single-cell_RNA-seq_Subject6_TEM_Cell032


title: Single-cell_RNA-seq_Subject6_TEM_Cell035


title: Single-cell_RNA-seq_Subject6_TEM_Cell033


title: Single-cell_RNA-seq_Subject6_TEM_Cell030


title: Single-cell_RNA-seq_Subject6_TEM_Cell029


title: Single-cell_RNA-seq_Subject6_TEM_Cell028


title: Single-cell_RNA-seq_Subject6_TEM_Cell027


title: Single-cell_RNA-seq_Subject6_TEM_Cell041


title: Single-cell_RNA-seq_Subject6_TEM_Cell025


title: Single-cell_RNA-seq_Subject6_TEM_Cell026


title: Single-cell_RNA-seq_Subject6_TEM_Cell024


title: Single-cell_RNA-seq_Subject6_TEM_Cell022


title: Single-cell_RNA-seq_Subject6_TEM_Cell020


title: Single-cell_RNA-seq_Subject6_TEM_Cell018


title: Single-cell_RNA-seq_Subject6_TEM_Cell023


title: Single-cell_RNA-seq_Subject6_TEM_Cell019


title: Single-cell_RNA-seq_Subject6_TEM_Cell021


title: Single-cell_RNA-seq_Subject6_TEM_Cell031


title: Single-cell_RNA-seq_Subject6_TEM_Cell016


title: Single-cell_RNA-seq_Subject6_TEM_Cell017


title: Single-cell_RNA-seq_Subject6_TEM_Cell014


title: Single-cell_RNA-seq_Subject6_TEM_Cell010


title: Single-cell_RNA-seq_Subject6_TEM_Cell013


title: Single-cell_RNA-seq_Subject6_TEM_Cell007


title: Single-cell_RNA-seq_Subject6_TEM_Cell004


title: Single-cell_RNA-seq_Subject6_TEM_Cell005


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source_name: Subject6_DENV positive_PBMC_TEMRA


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source_name: Subject6_DENV positive_PBMC_TCM


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source_name: Subject13_DENV positive_PBMC_TEMRA


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source_name: Subject13_DENV positive_PBMC_DENV antigen specific TEMRA


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source_name: Subject12_DENV positive_PBMC_TEM


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source_name: Subject12_DENV positive_PBMC_TEMRA


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title: Single-cell_RNA-seq_Subject12_TEMRA_Cell155


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell166


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell150


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell158


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell167


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell153


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell148


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell147


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell146


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell149


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell144


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell151


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell145


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell142


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell140


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell141


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell143


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell139


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell138


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell154


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell159


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell134


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell133


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell129


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell125


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell121


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell128


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell130


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell127


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell137


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell164


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell122


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell120


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell135


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell136


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell118


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell115


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell114


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell116


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell119


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell113


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell110


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell109


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell117


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell112


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell111


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell126


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell132


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell124


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell108


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell103


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell104


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell105


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell106


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell101


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell107


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell100


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell099


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell096


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell097


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell095


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell098


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell092


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell094


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell091


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell084


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell087


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell085


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell090


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell088


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell086


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell102


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell083


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell080


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell082


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell081


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell078


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell079


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell076


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell074


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell093


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell075


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell072


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell071


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell070


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell069


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell089


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell068


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell067


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell066


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell063


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell061


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title: Single-cell_RNA-seq_Subject12_TEMRA_Cell064


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell065


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell059


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell060


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell055


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell054


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell057


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell073


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell056


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell053


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell077


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell052


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell058


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell049


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell051


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell043


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell050


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell047


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell041


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell045


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell042


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell046


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell048


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell040


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell035


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell039


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell038


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell037


title: H3K4me3 ChIPseq in AtT-20 expressing Pax7

city: LAUSANNE

first: ALEXANDRE

last: MAYRAN

state: Waadt

address: Station 19

country: Switzerland

institute: EPFL

library_source: genomic

data_processing:
Sequence reads were trimmed for adapter sequences.
The resulting 50bp reads were trimmed to obtain high quality reads and then mapped to the mm10 mouse genome build using Bowtie with the following parameter: -t -p 4 --trim5 1 --best mm10 –S
Genome_build: mm10
Supplementary_files_format_and_content: bigwig files were generated using HOMER; Scores represent reads normalized per 10000000 reads

molecule: genomic DNA

organism: Mus musculus

source_name: AtT-20 cells expressing 3x flag-Pax7

growth_protocol: AtT-20 cells were plated on adherent culture dishes to obtain about 70% confluence after 3 days. They are cultured in DMEM + 10% FBS.

extract_protocol:
Nuclei lysates were sonicated and protein-DNA complexes were isolated with specific antibodies. Fragments of 200-400bp were kept for library preparation
Libraries were prepared according to Illumina's instructions. Libraries were sequenced on the Illumina Hi-seq 2000 following the manufacturer's protocols to obtain 50bp paired end reads.

tag: cell line

value: AtT-20 cells

value: pituitary corticotroph cell

tag: passages

tag: genotype/variation

value: expressing 3x flag-Pax7

tag: chip antibody

value: NEB 9751S


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell034


title: Ash2 ChIPseq in AtT-20 expressing Pax7

description: AtT-20 cells expressing 3x flag-Pax7

value: Bethyl A300-489A


title: H3K4me3 ChIPseq in AtT-20 expressing Neo

source_name: AtT-20 cells expressing 3x flag-Neo

value: expressing 3x flag-Neo


title: H3K27me3 ChIPseq in AtT-20 expressing Pax7


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell033

value: PBMC

value: TEMRA


title: H3K27me3 ChIPseq in AtT-20 expressing Neo


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell031

value: DENV positive


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell036


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell044


title: H3K9me2 ChIPseq in AtT-20 expressing Pax7


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell030


title: H3K9me2 ChIPseq in AtT-20 expressing Neo


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell027


title: H3K9me3 ChIPseq in AtT-20 expressing Neo

value: H3K9me3 millipore 07-442


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell029


title: Ash2 ChIPseq in AtT-20 expressing Neo

description: AtT-20 cells expressing 3x flag-Neo


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell025


title: SMC1 ChIPseq in AtT-20

source_name: AtT-20 cells

value: Bethyl A300-055A


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell026


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell023


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell024


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell028


title: CTCF ChIPseq in AtT-20 expressing Neo

value: CTCF millipore 07-729


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell032


title: SPF07

city: Philadelphia

first: Elizabeth

middle: A

last: Grice

state: PA

address: 1007 BRB II/III, 421 Curie Blvd

department: Dermatology

institute: University of Pennsylvania

description:
Sample_9983
Processed data file: gf_vs_spf_rna_seq.txt

data_processing:
Transcripts were aligned to the mouse reference genome GRCm38.p4 v9, using STAR in conjunction with AlignerBoost, specifying a seed length of 25, 4% seed mismatch, 0% seed indels, 8% all mismatch, and 3% all indels.
Reads mapping to numbered and sex chromosomes were retained using samtools.
Read counts were generated using featureCount in the subread package in R and counts to ribosomal RNA were removed.
Reads were filtered with the NOISeq R package using method 1, which removes features with a sum of expression values less than 1 count per million multiplied by the number of samples in the condition.
Post filtering, TMM normalization was applied and technical batch effects associated with sequencing run were removed using ARSyNseq, using the NOISeq R package.
Genome_build: GRCm38.p4 v9
Supplementary_files_format_and_content: Tab-delimited text files ending in "counts.txt" include count values for each Sample generated from R subread package; tab-delimited text files ending in "filtered_normalized_counts.txt" include counts for each sample, post filtering and normalization with NOISeq package in R; gf_vs_spf_rna_seq.txt is a tab-delimited text file containing filtered normalized counts for all 16 samples.

source_name: dorsum skin, SPF

extract_protocol:
Skin was collected from the dorsum of mice and stored in RNAlater. Poly-A enriched RNA was isolated from harvested skin and RNA-seq libraries were constructed using the unstranded Illumina TruSeq RNA Sample Prep Kit.
RNA libraries were prepared for sequencing using standard Illumina protocols.

tag: strain/background

tag: gender

value: male

tag: age

tag: condition

value: specific pathogen free

tag: tissue

value: dorsal skin


title: SPF06

description:
Sample_9982
Processed data file: gf_vs_spf_rna_seq.txt


title: GF08

description:
Sample_9979
Processed data file: gf_vs_spf_rna_seq.txt

source_name: dorsum skin, GF

value: germ free


title: SPF05

description:
Sample_9981
Processed data file: gf_vs_spf_rna_seq.txt


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell021

tag: subject id

tag: dengue infections history

tag: cell type


title: GF09

description:
Sample_9980
Processed data file: gf_vs_spf_rna_seq.txt


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell020

tag: cell subtype surface markers

tag: cell subtype


title: GF06

description:
Sample_9977
Processed data file: gf_vs_spf_rna_seq.txt


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell019


title: GF07

description:
Sample_9978
Processed data file: gf_vs_spf_rna_seq.txt


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell018


title: H3K9me3 ChIPseq in AtT-20 expressing Pax7


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell017


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell131


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell016


title: GF04

description:
Sample_6603
Processed data file: gf_vs_spf_rna_seq.txt


title: GF05

description:
Sample_6604
Processed data file: gf_vs_spf_rna_seq.txt


title: GF02

description:
Sample_6601
Processed data file: gf_vs_spf_rna_seq.txt


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell014


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell013


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell012


title: SPF03

description:
Sample_6598
Processed data file: gf_vs_spf_rna_seq.txt


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell010


title: SPF04

description:
Sample_6599
Processed data file: gf_vs_spf_rna_seq.txt


title: SPF02

description:
Sample_6597
Processed data file: gf_vs_spf_rna_seq.txt


title: SPF01

description:
Sample_6596
Processed data file: gf_vs_spf_rna_seq.txt


title: GF03

description:
Sample_6602
Processed data file: gf_vs_spf_rna_seq.txt


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell009

description: Whole Transcriptome Amplification (SmartSeq2 - Picelli et al.2014)


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell011


title: Single-cell_RNA-seq_Subject12_TEMRA_Cell008