title: 80S_5Adelcys_expB

city: Cambridge

first: Anthony

last: Schuller

address: 77 Massachusetts Avenue, 68-480

institute: Massachusetts Institute of Technology

description: ribosomal RNA

data_processing:
Trimmed of the 3′ adaptor using the skewer package
SN8 randomized nucleotides trimmed from read 5′ ends using fastx_trimmer
Reads were mapped to the yeast ribosomal rRNA using STAR to provide a normalized (reads per million, rpm) read 5' end count at each rRNA nucleotide
Divided the rpm at each rRNA nucleotide for each site-specific cysteine variant with the eIF5A Δcys or Upf1 Δ4cys controls
Genome_build: SacCer3 rRNA
Supplementary_files_format_and_content: wig files for each cys-variant cleaved rRNA

source_name: Purified ribosomes

extract_protocol:
The rRNA was then extracted by acid phenol, phenol:chloroform (5:1), and subsequently ethanol precipitated and resuspended in nuclease-free H2O.
In step 1, the cleaved rRNA is reverse transcribed (RT) using a random nonamer (SN8)-oligo (oAS544). 1 μg of cleaved rRNA was incubated with 20 μM oAS544 in 1X RT-Mg buffer (50 mM Tris-Cl, pH 8.6, 60 mM NaCl, 10 mM DTT) at 65oC for 5 minutes and then moved to ice to allow annealing of the oligo on the rRNA. Then 5 μL of this annealed-mixture was added to a mixture containing 2.5 μL 5X dNTP mix (1.7 mM of each dNTP), 2.5 μL 1X RT+Mg buffer (50 mM Tris-Cl, pH 8.6, 60 mM NaCl, 10 mM DTT, 6 mM MgCl2), and 2.5 μL RVT mix (0.25 μL 10X RT-Mg buffer, 0.25 μL SSIII reverse transcriptase (Thermo), and 2 μL H2O) to yield a final volume of 12.5 μL. This reaction was then reverse transcribed by incubating as follows: 25oC – 10 min, 50oC – 70 min, 60oC – 10 min, 4oC – indefinite. After the reaction completed, we incubated at 70oC for 15 min to denature the reverse transcriptase and then added 25 μL of 1X RT+Mg buffer to make a final volume of 37.5 μL. The reaction was then subjected to RNaseH digestion by adding 1 μL of RNAseH (NEB) and incubating at 37oC for 20 min to digest the rRNA.
In step 2, the resulting reverse transcription product is purified by RNAClean XP beads. We added 67.5 μL of beads to each reaction, and incubated at room temperature for 30 minutes, mixing the components every 10 minutes. Then the supernatant was removed after placing the reactions on a magnetic stand for 5 minutes. We subsequently washed with 200 μL of 70% EtOH two times, and eluted with 40 μL of 5 mM sodium citrate, pH 6.0. To elute, the beads were mixed with buffer and incubated at 37oC for 10 minutes, then placed on the magnetic stand and the eluant removed.
In step 3, the cDNA product is ligated with a 3′ linker for future sequencing steps. In this reaction, we mixed 3 μL of each cDNA product with a 7 μL ligation mix containing 1 μL 10X Circligase buffer, 0.5 μL 1 mM ATP, 0.5 μL 50 mM MnCl2, 2 μL 50% PEG 6000, 2 μL 5 M betaine, 0.5 μL of 100 μM oAS545 linker, and 0.5 μL Circligase enzyme. The resulting 10 μL reaction was incubated as follows: 60oC – 120 min, 68oC – 60 min, 80oC – 10 min, 4oC – indefinite. Then we added 10 μL H2O for a final volume of 20 μL to proceed.
In step 4, this ligated cDNA product is purified using Ampure XP beads. The procedure is similar to step 2 with the following exceptions: 1) We used 36 μL of beads to clean the 20 μL sample, and 2) We eluted using 16 μL of H2O.
In step 5, the purified cDNA is amplified using PCR primers containing adaptor sequnces for Illumina sequencing and barcodes for sample multiplexing. Our PCR reactions contained 6 μL of ligated cDNA product, 3.6 μL of 10 μM Index oligo (oASBar01-30), 3.6 μL of 10 μM forward oligo oBZ287, 12 μL 5X Phusion HF Buffer, 4.8 μL 2.5 mM dNTPs, 28.8 μL H2O, and 1.2 μL Phusion DNA polymerase. Our amplification protocol was as follows: 98oC – 3 min, (98oC – 80 sec, 64oC – 15 sec, 72oC – 30 sec) repeated 4 times, (98oC – 80 sec, 72oC – 45 sec) repeated 10 times, 72oC – 5 min, 4oC – indefinite. The PCR reactions were then run on an 8% TBE native gel at 200V for 28min and stained with SYBR gold.  The resulting products > 150 bp were cut from the gel and extracted overnight in 0.3 M NaOAc with 1 mM ETDA at room temperature. We intentionally cut >150 bp to avoid sequencing the amplified product that results from ligation of the unextended RT oligo directly to the 3′ linker (and therefore contains no insert cDNA). The following day, the extracted DNA was precipitated with isopropanol and quantified using an Agilent 2100 Bioanalyzer before sequencing.

treatment_protocol: Reactions were incubated on ice for 15 minutes to allow factor binding. Then we added 1 μL of 100 mM Ascorbic Acid, and 1 μL fresh 0.5% hydrogen peroxide simultaneously to initiate radical generation. Pipetting each 1 μL reactant on the wall of a microcentrifuge tube, and then quickly spinning the contents allowed for simultaneous mixing. The reaction continued on ice for 15 minutes and was then quenched by addition of 1 μL of 100mM thiourea and 300 μL of rRNA extraction buffer (0.3 M NaOAc).

tag: treatment time

value: 15 minutes

tag: concentration ribosomes

tag: concentration protein


title: 80S_Upf1_del4cys_expB

status: Public on Dec 06 2017

type: SRA

state: MA

department: Biology

country: USA

description: ribosomal RNA

library_source: transcriptomic

molecule: total RNA

organism: Saccharomyces cerevisiae


title: ESO7-EC_rep2_RNA-seq

city: Stanford

first: Joseph

last: Wu

state: Carlifornia

address: Stanford University School of Medicine

institute: Stanford University School of Medicine

data_processing:
hisat2 -x hg38.idx -U ESO7-CM_rep1.fastq.gz -S ESO7-CM_rep1.sam -p 2
featureCounts -T 2 -t exon -g gene_name -s 1 -a hg38_P2_ERCC.gtf -o MingTao_NT_mRNA.txt 12C-CM_rep1_sorted.bam 12C-CM_rep2_sorted.bam 12C-EC_rep1_sorted.bam
trim_galore --paired ESO7-CM_R1.fq.gz ESO7-CM_R2.fq.gz
bismark bt2_hg38 --bowtie2 -1 ESO7-CM_R1_val_1.fq.gz -2 ESO7-CM_R2_val_2.fq.gz
MethylKit
Genome_build: hg38

organism: Homo sapiens

source_name: IVF-ESC-ECs

growth_protocol: Pluripotent stem cells (iPSC, nt-ESCs, and IVF-ESCs) were maintained in chemically defined E8 medium on Matrigel-coated plates. PSC-derived cardiomyocytes (PSC-CMs) were cultured in RPMI-1640 medium plus B27 supplement on Matrigel-coated plates. PSC-derived endothelial cells (PSC-ECs) were maintained in EGM2 medium on 0.2% gelatin-coated plates.

extract_protocol:
Total RNAs were extracted by RNeasy Plus Mini kit (Qiagen, cat no. 74134) according to the manufacturer’s protocol. Genomic DNA was isolated using a DNeasy Blood and Tissue kit (Qiagen, cat no. 69506).
For RNA-seq library preparation, mRNAs were isolated from total RNAs using the Dynabeads Oligo (dT)25 in a Dynabeads mRNA DIRECT Micro Kit (Thermo Fisher Scientific, cat no. 61021). ERCC Spike-In Control Mix (Thermo Fisher Scientific, cat no. 4456740) was added to total RNA input before mRNA isolation. RNA-seq libraries were constructed using an Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific, cat no. 4479789). For RRBS-seq library construction, 300 ng of genomic DNA was digested with MspI (NEB, cat no. R0106S) at 37°C for 1 hr. Unmethylated lambda DNA (Promega, cat no. D1521) was spiked in as the quality control for sodium bisulfite conversion. The DNA fragments were then purified and selected using Agencourt AMPure XP beads (Beckman Coulter, cat no. A63880). DNA fragments were repaired to blunt ends by Klenow fragments (NEB, cat no. M0212S). DNA fragments were then ligated to the methylated Illumina adapters (NEB, cat no. E7535L) using a NEBNext Ultra Ligation Module (NEB, cat no. E7445S). The ligation product was purified using Agencourt AMPure XP beads and subsequently subjected to sodium bisulfite conversion using an EZ DNA Methylation-Gold Kit (Zymo Research, cat no. D5005). The purified bisulfite-converted DNA was amplified using the EpiMark Hot Start Taq DNA Polymerase (NEB, cat no. M0490). NEBNext Index Primers were used to barcode different samples during PCR amplification.
RNA-seq and RRBS-seq

treatment_protocol: Different reprogramming mechanisms: iPSCs, somatic cell nuclear transfer, and IVF.

tag: cell type

value: Endothelial cells

tag: passage


title: 80S_5A_K48C_expA

description: ribosomal RNA


title: 80S_5A_T126C_expA

description: ribosomal RNA


title: 80S_Upf1_del4cys_expD

description: ribosomal RNA

value: 16 minutes


title: ESO7-CM_rep1_RNA-seq

source_name: IVF-ESC-CMs

value: Cadiomyocytes

value: Day 30


title: 80S_Upf1_S335C_expD

description: ribosomal RNA


title: 80S_Upf1_T337C_rep_expC

description: ribosomal RNA


title: 80S_Upf1_T337C_expC

description: ribosomal RNA


title: 80S_Upf1_G506C_expC

description: ribosomal RNA


title: 80S_Upf1_del4cys_expC

description: ribosomal RNA


title: ESO7-CM_rep2_RNA-seq


title: ESO8-EC_rep1_RNA-seq


title: T3(small yellow follicle with higher expression level of FSHR)

city: Tai'an

first: Wang

last: Yiya

state: Shandong

address: Daizong Street no.61

country: China

institute: Shandong Agricultural University

data_processing:
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm8 whole genome using bowtie v0.12.2 with parameters -q -p 4 -e 100 -y -a -m 10 --best --strata
Cufflinks are used for assemble transcripts and remap and quanlity gene-expression level, which is estimate by FPKM value.
Genome_build: Gallus_gallus-5.0
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample ...

organism: Gallus gallus

source_name: Chicken follicle

extract_protocol:
RNA was extracted from follicle samples (stored at liquid nitrogen) according to the EZNA Tissue RNA Kit instruction manual. RNA sequencing libraries were generated using the NEB Next Ultra RNA Library Prep Kit for Illumina (Cat# E7420L).
RNA libraries were prepared for sequencing using standard Illumina protocols

tag: tissue

value: follicle

tag: age

value: 30 weeks

tag: genotype

value: wild type


title: 80S_5A_K48C_expB

description: ribosomal RNA


title: 80S_Upf1_S335C_expB

description: ribosomal RNA


title: T6(small yellow follicle with lower expression level of FSHR)


title: T5(small yellow follicle with lower expression level of FSHR)


title: 80S_5Adelcys_expA

description: ribosomal RNA


title: Brg1+/+ mTEClo 2 [ATAC-Seq]

first: Andrew

middle: S.

last: Koh

state: CA

address: 279 Campus Drive B203

institute: Stanford University

library_source: genomic

data_processing:
Reads were trimmed with a custom script (Buenrostro et al., 2015), aligned using Bowtie2 and filtered for unique reads with alignment quality > q30.  Reads mapping to mitochondrial, ChrY and unmapped contigs were removed.
Peaks were called using MACS2 and filtered with a custom blacklist (Buenrostro et al., 2015).  Peak summits were extended +/- 250-bp and filtered for non-overlapping, maximally significant 500-bp peaks.
ATAC-seq fragment counts for each sample were calculated across all peaks, quantile normalized and GC-content normalized using the R package CQN as previously described (Buenrostro et al., 2015; Corces et al., 2016).
Genome_build: mm9
Supplementary_files_format_and_content: Tab-delimited text file with normalized fragment counts for corresponding peaks (rows) and samples (columns).

molecule: genomic DNA

organism: Mus musculus

source_name: Thymus

extract_protocol:
60,000 cells were sorted into RSB buffer without detergent (10mM Tris 7.4, 10mM NaCl, 3mM MgCl2).  Cells were resuspended in 200ul RSB buffer with 0.1% Tween-20 and incubated on ice for 5 min. Cells were pelleted, resuspended in 50ul TD buffer with 2.5ul Tn5 transposase (Illumina) and incubated at 37 degrees for 30 min. Transposition reactions were cleaned up with MinElute columns (Qiagen).
Libraries were constructed as previously described (Buenrostro et al., 2013).

treatment_protocol: Thymi were tissue-digested, enriched for stroma and sorted for DAPIneg, CD45neg, Epcam+, UEA+, Ly51lo, MHCIIhi and MHCIIlo mTECs

value: mTEClo

value: 4 weeks

tag: strain


title: Brg1-/- mTEClo 2 [ATAC-Seq]


title: Brg1-/- mTEClo 1 [ATAC-Seq]


title: T1(small yellow follicle with higher expression level of FSHR)


title: 80S_5A_T126C_expB

description: ribosomal RNA


title: Brg1+/+ mTEClo 1 [ATAC-Seq]


title: Brg1-/- mTEChi 2 [ATAC-Seq]

value: mTEChi


title: Brg1-/- mTEChi 1 [ATAC-Seq]


title: Brg1+/+ mTEChi 2 [ATAC-Seq]


title: Brg1+/+ mTEChi 1 [ATAC-Seq]


title: Aire+/+ mTEChi 1 [ATAC-Seq]

value: Aire+/+


title: Aire+/+ mTEClo 2 [ATAC-Seq]


title: Aire+/+ mTEChi 2 [ATAC-Seq]


title: Aire-/- mTEClo 2 [ATAC-Seq]

value: Aire-/-


title: Aire+/+ mTEClo 1 [ATAC-Seq]


title: Aire-/- mTEChi 2 [ATAC-Seq]


title: Aire-/- mTEChi 1 [ATAC-Seq]


title: WG960021_H10-W27

city: Stockholm

first: Sten

last: Linnarsson

address: Scheeles väg 1

department: Medical Biochemistry and Biophysics

country: Sweden

institute: Karolinska Institutet

description: Processed data file: molecules_mouse.xlsx

data_processing:
Reads flagged as invalid by the Illumina HiSeq control software were discarded. In the remaining reads, any 3′ bases with a quality score of B were removed. If any of the six UMI bases in the 5' end had a Phred score <17 the read was discarded, else the UMI was cut away and saved. The following three bases had to be G for the read to be kept. These, as well as any additional up to totally nine (presumably template-switch derived) G:s were cut away. Reads were discarded if the remaining transcript-derived sequence ended in a poly(A) sequence leaving <25 bases, or if the remaining sequence consisted of fewer than six non-A bases or a dinucleotide repeat with fewer than six other bases at either end. The reads were sorted by the well, as defined by the combined two index read barcodes.
Alignment was performed using the Bowtie aligner, allowing for up to three mismatches and up to 24 alternative mappings. Reads with no alignments were realigned against an artificial chromosome, containing all possible splice junctions arising from the exons defined by the UCSC transcript models. The coordinates of aligned splice junctions were translated back to the corresponding genomic positions.
For expression level calculation, the exons of each locus with several transcript variants were merged to a combined model representing total expression of the locus. To account for incomplete cap site knowledge, the 5′ ends of all models were extended by 100 bases, but not beyond the 3′ end of any upstream nearby exon of another gene of the same orientation.
The annotation step was performed separately for each well. For each genomic position and strand combination the number of reads in each UMI was counted. Any multiread that mapped to some repeat outside exons was assigned randomly as one of these repeats and did not contribute to the transcript corresponding to the exon. Else, if the multiread mapped to some exon, and not to any repeat outside exons, it was assigned to the exon where it was closest to the transcript model 5′ end. If it had no exon mapping, it was assigned randomly at one of the mappings. The total number of molecules at each mapping position was determined by the number of distinct UMIs observed. Any UMI represented by only a single read was excluded, in order to reduce false molecules due to PCR and sequencing errors. The raw UMI count was corrected for the UMI collision probability. For the human samples all reads mapping anywhere within the whole locus, defined as the region (including actual introns) from the start of the 100 base 5'end extension of the first exon up to the end of the last exon, were counted as exon-derived.
Genome_build: UCSC mm10 (GRCm38) and hg19 (GRCh37)
Supplementary_files_format_and_content: *xlsx: Excel tables of mRNA molecule counts with genes in rows and cells in columns.

source_name: somatosensory cortex

growth_protocol: Male and female wild type CD-1 mice (Charles River) between postnatal days 21-37 were used. Postmortem human brain tissue was provided to the Allen Institute for Brain Science by the San Diego Medical Examiner’s (SDME) office.

extract_protocol:
Mice were anesthetized with isoflurane, perfused with ice-cold aCSF, brains collected and sectioned using a vibratome or brain matrix and the somatosensory cortex was microdissected. Single cell suspensions were generated using the Worthington Papain dissociation system. Human nuclei were isolated from a -80°C frozen tissue piece using Dounce homogenization.
50nl lysis mix (500nM STRT-P1-T31, 4.5nM dNTP, 2% Triton-X-100, 20mM DTT, 1.5U/μl TaKaRa RNase Inhibitor) was dispensed, followed by 3min lysis at 72°C. Then, 85nl reverse transcription (RT) mix (2.1X SuperScript II First-Strand Buffer, 12.6mM MgCl2, 1.79M betaine, 14.7U/μl SuperScript II, 1.58U/μl TaKaRa RNase Inhibitor, 10.5μM P1B-UMI-RNA-TSO) were dispensed and RT carried out 42°C for 90 minutes. 96 Transposome stocks were assembled (6.25μM barcoded adapter (STRT-Tn5-Idx[1-96]), 6.25μM Tn5 transposase, 40% glycerol), 37°C 1h. Tn5 reactions were assembled with 3μl transposome and 2μl amplified cDNA, in a total 20μl 1x CutSmart buffer (NEB), and incubated at 55°C 20min.

tag: cluster

value: mgl

value: somatosensory cortex

tag: strain/background


title: WG960021_H12-W02

value: mol


title: WG960021_H10-W28


title: WG960021_H10-W30

value: astro


title: WG960021_H10-W16


title: WG960021_H12-W08


title: WG960021_H11-W22


title: WG960021_H11-W21


title: WG960021_H11-W15


title: WG960021_H10-W19


title: WG960021_H10-W29


title: WG960021_H10-W14


title: WG960021_H09-W28


title: WG960021_H10-W05


title: WG960021_H09-W25


title: WG960021_H09-W21


title: WG960021_H09-W24


title: WG960021_H09-W16


title: Aire-/- mTEClo 1 [ATAC-Seq]


title: WG960021_H09-W08


title: WG960021_H10-W17


title: WG960021_H08-W16


title: WG960021_H08-W19


title: WG960021_H07-W25


title: WG960021_H07-W31


title: WG960021_H07-W03


title: WG960021_H05-W14


title: WG960021_H04-W15


title: WG960021_H07-W02

value: cop


title: WG960021_H03-W30


title: WG960021_H03-W22


title: WG960021_H04-W11


title: WG960021_H03-W23


title: WG960021_H03-W25


title: WG960021_H01-W11


title: WG960021_G12-W21


title: WG960021_H03-W20


title: WG960021_G12-W19


title: WG960021_H01-W19


title: WG960021_G12-W18

value: int


title: WG960021_H01-W15


title: WG960021_G12-W17


title: WG960021_H01-W06


title: WG960021_H04-W12


title: WG960021_H01-W01


title: WG960021_G12-W08


title: WG960021_G12-W12


title: WG960021_G12-W05


title: WG960021_G12-W02


title: WG960021_H07-W24


title: WG960021_G11-W26


title: WG960021_G11-W10


title: WG960021_G11-W13


title: WG960021_G10-W32


title: WG960021_G10-W24


title: WG960021_G10-W29


title: WG960021_G09-W24


title: WG960021_G10-W17


title: WG960021_G09-W20


title: WG960021_G09-W11

value: nfol


title: WG960021_G09-W03


title: WG960021_G09-W16


title: WG960021_G08-W17


title: WG960021_G08-W29


title: WG960021_G09-W04


title: WG960021_G08-W01


title: WG960021_G07-W25


title: WG960021_G07-W26

value: opc


title: WG960021_G07-W09


title: WG960021_G06-W25


title: WG960021_G07-W23


title: WG960021_G11-W03


title: WG960021_G08-W09


title: WG960021_G07-W02


title: WG960021_G06-W18


title: WG960021_G07-W07


title: WG960021_G06-W03


title: WG960021_G06-W15


title: WG960021_G05-W17


title: WG960021_G05-W31

value: vsm


title: WG960021_G06-W10


title: WG960021_G09-W10


title: WG960021_G05-W14


title: WG960021_G05-W10


title: WG960021_G04-W28


title: WG960021_G05-W05


title: WG960021_G04-W27


title: WG960021_G07-W16


title: WG960021_G07-W15


title: WG960021_G03-W10


title: WG960021_G03-W13


title: WG960021_G03-W03


title: WG960021_G03-W01


title: WG960021_G02-W17


title: WG960021_G02-W06


title: WG960021_G02-W11


title: WG960021_G02-W16


title: WG960021_G03-W09


title: WG960021_G02-W03


title: WG960021_G02-W04


title: WG960021_G03-W12


title: WG960021_G01-W02


title: WG960021_F12-W26


title: WG960021_F12-W30


title: WG960021_G02-W12


title: WG960021_G01-W05


title: WG960021_F12-W23


title: WG960021_F12-W10


title: WG960021_G04-W16


title: WG960021_F12-W08


title: WG960021_F12-W09


title: WG960021_G04-W13


title: WG960021_F12-W20


title: WG960021_F11-W22

value: peric


title: WG960021_G04-W06


title: WG960021_F11-W21


title: WG960021_G02-W14


title: WG960021_F11-W14


title: WG960021_F11-W17


title: WG960021_F11-W11


title: WG960021_F11-W03


title: WG960021_F10-W25


title: WG960021_F10-W27


title: WG960021_F10-W15


title: WG960021_F10-W11


title: WG960021_F09-W22


title: WG960021_F10-W02


title: WG960021_F09-W13


title: WG960021_F09-W26


title: WG960021_F11-W30


title: WG960021_F09-W07


title: WG960021_F09-W06


title: WG960021_F12-W02


title: WG960021_F08-W16


title: WG960021_F07-W30


title: WG960021_F07-W25


title: WG960021_F11-W10


title: WG960021_F08-W14


title: WG960021_F08-W20


title: WG960021_F07-W20


title: WG960021_F08-W07


title: WG960021_F07-W19


title: WG960021_F07-W10


title: WG960021_F10-W20


title: WG960021_F07-W06


title: WG960021_F07-W15


title: WG960021_F07-W05


title: WG960021_F06-W06


title: WG960021_F11-W04


title: WG960021_F06-W04


title: WG960021_F05-W15


title: WG960021_F05-W03


title: WG960021_F05-W24


title: WG960021_F05-W26


title: WG960021_F04-W17


title: WG960021_F04-W12


title: WG960021_F04-W05


title: WG960021_F04-W31


title: WG960021_F05-W02


title: WG960021_F08-W02


title: WG960021_F04-W10


title: WG960021_F03-W24


title: WG960021_F03-W10


title: WG960021_F01-W23


title: WG960021_F03-W08


title: WG960021_E12-W23


title: WG960021_E12-W25

value: mfol


title: WG960021_E12-W29


title: WG960021_E12-W17


title: WG960021_E12-W27


title: WG960021_E12-W18


title: WG960021_E12-W01


title: WG960021_E11-W21


title: WG960021_E11-W14


title: WG960021_E11-W10


title: WG960021_F03-W32


title: WG960021_E12-W12


title: WG960021_E10-W14

value: endo


title: WG960021_E11-W03


title: WG960021_E10-W30


title: WG960021_E09-W28


title: WG960021_E09-W17


title: WG960021_E09-W15


title: WG960021_E09-W21


title: WG960021_F01-W09


title: WG960021_E09-W13


title: WG960021_E09-W11


title: WG960021_E09-W07


title: WG960021_E09-W05


title: WG960021_E09-W02


title: WG960021_E12-W04


title: WG960021_E08-W30


title: WG960021_E08-W27


title: WG960021_E08-W25


title: WG960021_E08-W18


title: WG960021_E11-W18


title: WG960021_E08-W11


title: WG960021_E08-W05


title: WG960021_E07-W16


title: WG960021_E07-W11


title: WG960021_E10-W04


title: WG960021_E09-W20


title: WG960021_E11-W06


title: WG960021_E07-W10


title: WG960021_E09-W08


title: WG960021_E07-W05


title: WG960021_E06-W14


title: WG960021_E06-W16


title: WG960021_E06-W11


title: WG960021_E06-W09


title: WG960021_E06-W12


title: WG960021_E06-W07


title: WG960021_E09-W04


title: WG960021_E05-W07


title: WG960021_E05-W10


title: WG960021_E06-W02


title: WG960021_E04-W02


title: WG960021_E04-W10


title: WG960021_E03-W15


title: WG960021_E04-W16


title: WG960021_E02-W14


title: WG960021_E02-W02


title: WG960021_E01-W20

value: rbc


title: WG960021_E01-W09


title: WG960021_D11-W26


title: WG960021_D11-W18


title: WG960021_D11-W15


title: WG960021_E06-W17


title: WG960021_E05-W24


title: WG960021_E06-W13


title: WG960021_D09-W29


title: WG960021_D11-W13


title: WG960021_D09-W17


title: WG960021_D09-W23


title: WG960021_D07-W07


title: WG960021_D07-W31


title: WG960021_D07-W24


title: WG960021_D07-W16


title: WG960021_D09-W28


title: WG960021_D05-W21


title: WG960021_D05-W16


title: WG960021_D03-W19


title: WG960021_D03-W16


title: WG960021_D03-W27


title: WG960021_D03-W03


title: WG960021_D03-W06


title: WG960021_D05-W10


title: WG960021_D03-W02


title: WG960021_D01-W28


title: WG960021_D03-W26


title: WG960021_D01-W19


title: WG960021_D01-W16


title: WG960021_D01-W25


title: WG960021_E08-W13


title: WG960021_D01-W14


title: WG960021_C12-W31


title: WG960021_C12-W06


title: WG960021_C12-W28


title: WG960021_C12-W21


title: WG960021_C11-W19


title: WG960021_C11-W23


title: WG960021_C12-W23


title: WG960021_C12-W03


title: WG960021_C11-W16


title: WG960021_C09-W04


title: WG960021_C08-W16


title: WG960021_C11-W15


title: WG960021_C11-W17


title: WG960021_C07-W22


title: WG960021_C07-W12


title: WG960021_C07-W07


title: WG960021_C06-W23


title: WG960021_C06-W14


title: WG960021_C07-W06


title: WG960021_C06-W31


title: WG960021_C05-W31


title: WG960021_C05-W19


title: WG960021_C05-W29


title: WG960021_C05-W16


title: WG960021_C04-W27


title: WG960021_C04-W26


title: WG960021_C05-W08


title: WG960021_C11-W13


title: WG960021_C04-W18


title: WG960021_C04-W17


title: WG960021_C04-W15


title: WG960021_C03-W31


title: WG960021_C04-W06


title: WG960021_C03-W12


title: WG960021_C03-W25


title: WG960021_C03-W20


title: WG960021_C03-W09


title: WG960021_C03-W08


title: WG960021_C02-W16


title: WG960021_C03-W30


title: WG960021_C03-W03


title: WG960021_C01-W28


title: WG960021_C02-W10


title: WG960021_C05-W09


title: WG960021_C01-W15


title: WG960021_C01-W06


title: WG960021_C04-W22


title: WG960021_C01-W12


title: WG960021_C01-W07


title: WG960021_C01-W09


title: WG960021_B12-W31


title: WG960021_B12-W25


title: WG960021_B12-W07


title: WG960021_B12-W03


title: WG960021_B12-W06


title: WG960021_B11-W29


title: WG960021_B11-W19


title: WG960021_B11-W27


title: WG960021_B11-W14


title: WG960021_B10-W20


title: WG960021_B11-W03


title: WG960021_B10-W21


title: WG960021_B10-W10


title: WG960021_B11-W26


title: WG960021_B10-W29


title: WG960021_B11-W24


title: WG960021_B10-W06


title: WG960021_B10-W08


title: WG960021_B10-W18


title: WG960021_B10-W13


title: WG960021_B10-W03


title: WG960021_B09-W32


title: WG960021_B09-W28


title: WG960021_B09-W20


title: WG960021_B09-W17


title: WG960021_B08-W31


title: WG960021_B09-W08


title: WG960021_B09-W16


title: WG960021_B08-W19


title: WG960021_B08-W17


title: WG960021_B08-W15


title: WG960021_B11-W05


title: WG960021_B08-W13


title: WG960021_B08-W07


title: WG960021_B07-W26


title: WG960021_B08-W04


title: WG960021_B08-W10


title: WG960021_B07-W30


title: WG960021_B07-W15


title: WG960021_B07-W12


title: WG960021_B07-W11


title: WG960021_B07-W16


title: WG960021_B06-W24


title: WG960021_B07-W22


title: WG960021_B06-W28


title: WG960021_B09-W09


title: WG960021_B05-W16


title: WG960021_B05-W15


title: WG960021_B05-W11


title: WG960021_B05-W12


title: WG960021_B05-W13


title: WG960021_B04-W29


title: WG960021_B05-W28


title: WG960021_B04-W26


title: WG960021_B04-W12


title: WG960021_B04-W25


title: WG960021_B04-W15


title: WG960021_B04-W24


title: WG960021_B04-W20


title: WG960021_B05-W10


title: WG960021_B03-W24


title: WG960021_B04-W05


title: WG960021_B03-W15


title: WG960021_B02-W29


title: WG960021_B06-W03


title: WG960021_B04-W07


title: WG960021_B02-W13


title: WG960021_B02-W12


title: WG960021_B01-W16


title: WG960021_B01-W20


title: WG960021_B02-W07


title: WG960021_B02-W15


title: WG960021_B02-W05


title: WG960021_B01-W27


title: WG960021_A12-W14


title: WG960021_A12-W13


title: WG960021_B01-W26


title: WG960021_A12-W31


title: WG960021_A12-W04


title: WG960021_B01-W17


title: WG960021_A12-W30


title: WG960021_A11-W26


title: WG960021_A12-W28


title: WG960021_A10-W29


title: WG960021_A11-W25


title: WG960021_A11-W17


title: WG960021_A12-W07

value: pvm


title: WG960021_A11-W20


title: WG960021_B01-W08


title: WG960021_A11-W06


title: WG960021_A11-W29


title: WG960021_A10-W27


title: WG960021_A10-W32


title: WG960021_A11-W23


title: WG960021_A09-W28


title: WG960021_A09-W21


title: WG960021_A10-W02


title: WG960021_A10-W18


title: WG960021_A12-W24


title: WG960021_A08-W31


title: WG960021_A11-W32


title: WG960021_A09-W29


title: WG960021_A08-W17


title: WG960021_A09-W10


title: WG960021_A07-W18


title: WG960021_A06-W27


title: WG960021_A07-W26


title: WG960021_A06-W25


title: WG960021_A06-W12


title: WG960021_A05-W29


title: WG960021_A08-W18


title: WG960021_A07-W32


title: WG960021_A07-W15


title: WG960021_A06-W04


title: WG960021_A06-W01


title: WG960021_A05-W32


title: WG960021_A05-W16


title: WG960021_A05-W15


title: WG960021_A05-W13


title: WG960021_A08-W21


title: WG960021_A09-W09


title: WG960021_A05-W04


title: WG960021_A05-W28


title: WG960021_A05-W18


title: WG960021_A04-W21


title: WG960021_A08-W12


title: WG960021_A04-W16


title: WG960021_A04-W07


title: WG960021_A04-W30


title: WG960021_A03-W32


title: WG960021_A03-W30


title: WG960021_A03-W29


title: WG960021_A03-W25


title: WG960021_A03-W15


title: WG960021_A03-W24


title: WG960021_A03-W14


title: WG960021_A03-W09


title: WG960021_A02-W09


title: WG960021_A05-W11


title: WG960021_A03-W03


title: WG960021_A02-W19


title: WG960021_A02-W04


title: WG960021_A01-W14


title: WG960021_A01-W29


title: WG960021_A04-W13


title: WG960017_H12-W24


title: WG960021_A01-W11


title: WG960021_A01-W20


title: WG960021_A01-W05


title: WG960021_A01-W06


title: WG960017_H11-W22


title: WG960017_H11-W29


title: WG960017_H11-W10


title: WG960017_H11-W13


title: WG960017_H11-W06


title: WG960021_A03-W22


title: WG960017_H11-W05


title: WG960017_H10-W15


title: WG960017_H11-W01


title: WG960017_H10-W11


title: WG960017_H10-W12


title: WG960017_H10-W06


title: WG960017_H10-W08


title: WG960017_H09-W28


title: WG960017_H10-W05


title: WG960021_A01-W15


title: WG960017_H11-W32


title: WG960017_H09-W17


title: WG960017_H09-W09


title: WG960017_H09-W11


title: WG960017_H12-W13


title: WG960017_H11-W07


title: WG960017_H09-W15


title: WG960017_H08-W10


title: WG960017_H09-W13


title: WG960017_H08-W06


title: WG960017_H09-W10


title: WG960017_H08-W05


title: WG960017_H08-W04


title: WG960017_H07-W27


title: WG960017_H07-W28


title: WG960017_H08-W01


title: WG960017_H07-W22


title: WG960017_H07-W25


title: WG960017_H07-W19


title: WG960017_H09-W21


title: WG960017_H07-W01


title: WG960017_H07-W12


title: WG960017_H06-W13


title: WG960017_H06-W01


title: WG960017_H05-W12


title: WG960017_H05-W11


title: WG960017_H04-W26


title: WG960017_H05-W09


title: WG960017_H04-W25


title: WG960017_H04-W18


title: WG960017_H04-W01


title: WG960017_H04-W06


title: WG960017_H03-W24


title: WG960017_H04-W09


title: WG960017_H03-W21


title: WG960017_H02-W08


title: WG960017_H02-W06


title: WG960017_H07-W17


title: WG960017_H06-W04


title: WG960017_H02-W03


title: WG960017_H06-W10


title: WG960017_H01-W09


title: WG960017_H06-W02


title: WG960017_G12-W29


title: WG960017_H05-W10


title: WG960017_G12-W19


title: WG960017_G12-W27


title: WG960017_G12-W28


title: WG960017_H04-W14


title: WG960017_G12-W16


title: WG960017_G12-W08


title: WG960017_G11-W31


title: WG960017_G11-W32


title: WG960017_G11-W28


title: WG960017_G12-W07


title: WG960017_G11-W25


title: WG960017_H03-W03


title: WG960017_G11-W23


title: WG960017_H02-W17


title: WG960017_G11-W22


title: WG960017_G11-W27


title: WG960017_H01-W30


title: WG960017_G11-W20


title: WG960017_H01-W08


title: WG960017_G11-W17


title: WG960017_G11-W21


title: WG960017_G11-W16


title: WG960017_G11-W13


title: WG960017_G11-W12


title: WG960017_G11-W11


title: WG960017_G11-W14


title: WG960017_G11-W09


title: WG960017_G11-W07


title: WG960017_G11-W06


title: WG960017_G10-W06


title: WG960017_G10-W15


title: WG960017_G10-W05


title: WG960017_G10-W03


title: WG960017_G11-W02


title: WG960017_G09-W22


title: WG960017_H02-W15


title: WG960017_G09-W20


title: WG960017_G08-W20


title: WG960017_G09-W12


title: WG960017_G09-W14


title: WG960017_G09-W11


title: WG960017_G08-W18


title: WG960017_G09-W17


title: WG960017_G08-W14


title: WG960017_G08-W15


title: WG960017_G08-W12


title: WG960017_G11-W04


title: WG960017_G09-W08


title: WG960017_G08-W13


title: WG960017_G08-W10


title: WG960017_G08-W06


title: WG960021_A12-W22


title: WG960017_G08-W02


title: WG960017_G07-W28


title: WG960017_G07-W17


title: WG960017_G08-W03


title: WG960017_G07-W13


title: WG960017_G07-W15


title: WG960017_G07-W22


title: WG960017_G07-W08


title: WG960017_G07-W05


title: WG960017_G07-W07


title: WG960017_G06-W12


title: WG960017_G06-W13


title: WG960017_G06-W14


title: WG960017_G06-W04


title: WG960017_G05-W17


title: WG960017_G06-W09


title: WG960017_G05-W07


title: WG960017_G04-W16


title: WG960017_G05-W03


title: WG960017_G05-W05


title: WG960017_G04-W15


title: WG960017_G04-W12


title: WG960017_G04-W09


title: WG960017_G04-W08


title: WG960017_G04-W02


title: WG960017_G02-W31


title: WG960017_G04-W01


title: WG960017_G02-W15


title: WG960017_F12-W22


title: WG960017_F12-W17


title: WG960017_F12-W14


title: WG960017_F12-W06


title: WG960017_F12-W12


title: WG960017_F12-W02


title: WG960017_F12-W08


title: WG960017_G06-W10


title: WG960017_F11-W29


title: WG960017_F11-W16


title: WG960017_F11-W26


title: WG960017_F11-W08


title: WG960017_F11-W01


title: WG960017_F10-W25


title: WG960017_F10-W29


title: WG960017_F10-W28


title: WG960017_F10-W26


title: WG960017_F10-W20


title: WG960017_F10-W14


title: WG960017_F10-W02


title: WG960017_F10-W01


title: WG960017_F10-W05


title: WG960017_F12-W05


title: WG960017_F10-W06


title: WG960017_F09-W31


title: WG960017_F09-W27


title: WG960017_F09-W30


title: WG960017_F09-W21


title: WG960017_F09-W20


title: WG960017_F09-W17


title: WG960017_F11-W15


title: WG960017_F09-W24


title: WG960017_F09-W26


title: WG960017_F09-W12


title: WG960017_F09-W11


title: WG960017_F09-W15


title: WG960017_F09-W03


title: WG960017_F09-W01


title: WG960017_F08-W30


title: WG960017_F08-W32


title: WG960017_F12-W13


title: WG960017_F10-W27


title: WG960017_F08-W13


title: WG960017_F08-W10


title: WG960017_F08-W05


title: WG960017_F08-W06


title: WG960017_F08-W04


title: WG960017_F08-W11


title: WG960017_F08-W03


title: WG960017_F07-W30


title: WG960017_F07-W27


title: WG960017_F07-W18


title: WG960017_F07-W17


title: WG960017_F07-W14


title: WG960017_F07-W11


title: WG960017_F06-W30


title: WG960017_F06-W29


title: WG960017_F07-W09


title: WG960017_F07-W28


title: WG960017_F06-W31


title: WG960017_F06-W27


title: WG960017_F06-W20


title: WG960017_F06-W13


title: WG960017_F07-W12


title: WG960017_F06-W26


title: WG960017_F06-W16


title: WG960017_F06-W11


title: WG960017_F06-W09


title: WG960017_F07-W06


title: WG960017_F08-W07


title: WG960017_F06-W12


title: WG960017_F06-W07


title: WG960017_F05-W20


title: WG960017_F06-W05


title: WG960017_F05-W19


title: WG960017_F05-W30


title: WG960017_F05-W17


title: WG960017_F05-W14


title: WG960017_F05-W13


title: WG960017_F05-W12


title: WG960017_F05-W10


title: WG960017_F05-W01


title: WG960017_F04-W31


title: WG960017_F04-W24


title: WG960017_F04-W18


title: WG960017_F04-W21


title: WG960017_F06-W10


title: WG960017_F04-W25


title: WG960017_F04-W28


title: WG960017_F04-W13


title: WG960017_F03-W10


title: WG960017_F04-W29


title: WG960017_F03-W04


title: WG960017_F04-W01


title: WG960017_F03-W20


title: WG960017_F06-W02


title: WG960017_F02-W19


title: WG960017_F04-W02


title: WG960017_F02-W22


title: WG960017_F03-W01


title: WG960017_F02-W14


title: WG960017_F02-W04


title: WG960017_F02-W11


title: WG960017_F02-W30


title: WG960017_F02-W03


title: WG960017_F02-W07


title: WG960017_F02-W02


title: WG960017_F02-W01


title: WG960017_F01-W02


title: WG960017_E12-W15


title: WG960017_F01-W01


title: WG960017_E12-W02


title: WG960017_E12-W06


title: WG960017_E11-W20


title: WG960017_E11-W25


title: WG960017_E11-W16


title: WG960017_E11-W18


title: WG960017_E11-W19


title: WG960017_E11-W10


title: WG960017_E11-W12


title: WG960017_F02-W06


title: WG960017_E11-W09


title: WG960017_E10-W23


title: WG960017_E10-W29


title: WG960017_E11-W27


title: WG960017_E10-W24


title: WG960017_E11-W03


title: WG960017_E11-W02


title: WG960017_E11-W08


title: WG960017_E10-W12


title: WG960017_E10-W06


title: WG960017_E10-W01


title: WG960017_E10-W21


title: WG960017_E10-W08


title: WG960017_E09-W29


title: WG960017_E09-W26


title: WG960017_E09-W32


title: WG960017_E09-W22


title: WG960017_E09-W20


title: WG960017_E09-W14


title: WG960017_E09-W09


title: WG960017_E09-W05


title: WG960017_E09-W02


title: WG960017_E08-W16


title: WG960017_E09-W03


title: WG960017_E08-W12


title: WG960017_E08-W08


title: WG960017_E10-W16


title: WG960017_E08-W06


title: WG960017_E09-W15


title: WG960017_E07-W19


title: WG960017_E07-W25


title: WG960017_E08-W02


title: WG960017_E08-W25


title: WG960017_E07-W11


title: WG960017_E07-W12


title: WG960017_E07-W14


title: WG960017_E07-W13


title: WG960017_E09-W25


title: WG960017_E10-W14


title: WG960017_E07-W09


title: WG960017_E07-W10


title: WG960017_E08-W05


title: WG960017_E07-W08


title: WG960017_E09-W01


title: WG960017_E07-W07


title: WG960017_E07-W06


title: WG960017_E07-W02


title: WG960017_E06-W09


title: WG960017_E06-W12


title: WG960017_E07-W17


title: WG960017_E06-W05


title: WG960017_E05-W28


title: WG960017_E07-W16


title: WG960017_E06-W03


title: WG960017_E05-W22


title: WG960017_E05-W21


title: WG960017_E05-W26


title: WG960017_E05-W25


title: WG960017_E05-W20


title: WG960017_E09-W30


title: WG960017_E05-W14


title: WG960017_E05-W19


title: WG960017_E05-W09


title: WG960017_E05-W13


title: WG960017_E05-W08


title: WG960017_E05-W06


title: WG960017_E05-W04


title: WG960017_E07-W03


title: WG960017_E04-W25


title: WG960017_E04-W19


title: WG960017_E05-W29


title: WG960017_E05-W27


title: WG960017_E04-W12


title: WG960017_E04-W16


title: WG960017_E04-W18


title: WG960017_E04-W11


title: WG960017_E04-W02


title: WG960017_E03-W26


title: WG960017_E03-W14


title: WG960017_E03-W18


title: WG960017_E03-W27


title: WG960017_E03-W10


title: WG960017_E04-W26


title: WG960017_E03-W01


title: WG960017_E02-W26


title: WG960017_E02-W27


title: WG960017_E03-W04


title: WG960017_E02-W23


title: WG960017_E02-W09


title: WG960017_E02-W12


title: WG960017_E02-W29


title: WG960017_E02-W02


title: WG960017_E03-W05


title: WG960017_E01-W27


title: WG960017_E02-W16


title: WG960017_E04-W27


title: WG960017_E04-W05


title: WG960017_E01-W12


title: WG960017_E01-W08


title: WG960017_E01-W02


title: WG960017_E02-W06


title: WG960017_D11-W03


title: WG960017_D09-W32


title: WG960017_D11-W10


title: WG960017_D09-W20


title: WG960017_D09-W17


title: WG960017_D09-W26


title: WG960017_D09-W10


title: WG960017_D09-W07


title: WG960017_D08-W31


title: WG960017_D09-W05


title: WG960017_D07-W27


title: WG960017_D07-W18


title: WG960017_D07-W06


title: WG960017_E01-W14


title: WG960017_D07-W11


title: WG960017_D07-W01


title: WG960017_D05-W31


title: WG960017_D05-W26


title: WG960017_D05-W28


title: WG960017_D10-W12


title: WG960017_D10-W03


title: WG960017_D09-W13


title: WG960017_D05-W14


title: WG960017_D05-W15


title: WG960017_D05-W12


title: WG960017_D05-W24


title: WG960017_D06-W09


title: WG960017_D05-W16


title: WG960017_D05-W06


title: WG960017_D05-W02


title: WG960017_D04-W17


title: WG960017_D07-W26


title: WG960017_D07-W15


title: WG960017_D04-W13


title: WG960017_D04-W07


title: WG960017_D03-W28


title: WG960017_D03-W18


title: WG960017_D07-W10


title: WG960017_D09-W04


title: WG960017_D03-W06


title: WG960017_D06-W01


title: WG960017_D03-W12


title: WG960017_D02-W32


title: WG960017_D03-W01


title: WG960017_D02-W25


title: WG960017_D02-W21


title: WG960017_D02-W06


title: WG960017_C11-W18


title: WG960017_C12-W11


title: WG960017_C11-W09


title: WG960017_C10-W05


title: WG960017_C11-W02


title: WG960017_C09-W22


title: WG960017_C09-W10


title: WG960017_D03-W22


title: WG960017_C09-W15


title: WG960017_C08-W26


title: WG960017_C09-W02


title: WG960017_C09-W04


title: WG960017_C08-W32


title: WG960017_D02-W15


title: WG960017_C08-W10


title: WG960017_C08-W05


title: WG960017_C08-W21


title: WG960017_C07-W31


title: WG960017_C07-W32


title: WG960017_C07-W30


title: WG960017_C07-W28


title: WG960017_C07-W25


title: WG960017_C07-W23


title: WG960017_C07-W22


title: WG960017_C07-W17


title: WG960017_C07-W15


title: WG960017_C07-W19


title: WG960017_C07-W13


title: WG960017_C07-W07


title: WG960017_C09-W31


title: WG960017_C07-W09


title: WG960017_C07-W03


title: WG960017_C06-W28


title: WG960017_C07-W01


title: WG960017_C07-W06


title: WG960017_C07-W02


title: WG960017_C05-W16


title: WG960017_C05-W07


title: WG960017_C05-W15


title: WG960017_C05-W02


title: WG960017_C04-W21


title: WG960017_C04-W14


title: WG960017_C04-W08


title: WG960017_C06-W27


title: WG960017_C03-W20


title: WG960017_C03-W11


title: WG960017_C04-W02


title: WG960017_C03-W31


title: WG960017_C03-W24


title: WG960017_C04-W04


title: WG960017_C05-W13


title: WG960017_C02-W27


title: WG960017_C02-W01


title: WG960017_C02-W23


title: WG960017_C02-W05


title: WG960017_C01-W15


title: WG960017_C01-W16


title: WG960017_B12-W13


title: WG960017_B12-W15


title: WG960017_B12-W12


title: WG960017_B12-W10


title: WG960017_B12-W05


title: WG960017_B12-W04


title: WG960017_B12-W06


title: WG960017_B11-W05


title: WG960017_B11-W20


title: WG960017_B11-W04


title: WG960017_C03-W09


title: WG960017_B11-W02


title: WG960017_B10-W11


title: WG960017_B11-W03


title: WG960017_B10-W10


title: WG960017_B10-W09


title: WG960017_B10-W05


title: WG960017_B09-W31


title: WG960017_B09-W19


title: WG960017_B09-W16


title: WG960017_B09-W23


title: WG960017_B09-W14


title: WG960017_B09-W21


title: WG960017_B09-W04


title: WG960017_B09-W12


title: WG960017_B09-W11


title: WG960017_B08-W31


title: WG960017_B09-W09


title: WG960017_B08-W18


title: WG960017_B08-W11


title: WG960017_B08-W01


title: WG960017_B08-W03


title: WG960017_B12-W03


title: WG960017_B07-W30


title: WG960017_B07-W28


title: WG960017_B07-W26


title: WG960017_B07-W27


title: WG960017_B07-W25


title: WG960017_B07-W21


title: WG960017_B07-W16


title: WG960017_B07-W17


title: WG960017_B07-W15


title: WG960017_B07-W10


title: WG960017_B07-W05


title: WG960017_B07-W09


title: WG960017_B07-W08


title: WG960017_B06-W30


title: WG960017_B06-W29


title: WG960017_B06-W27


title: WG960017_B06-W28


title: WG960017_B06-W20


title: WG960017_B06-W03


title: WG960017_B06-W02


title: WG960017_B06-W15


title: WG960017_B05-W30


title: WG960017_B06-W13


title: WG960017_B05-W19


title: WG960017_B05-W28


title: WG960017_B06-W01


title: WG960017_B05-W26


title: WG960017_B07-W20


title: WG960017_B05-W10


title: WG960017_B05-W01


title: WG960017_B04-W30


title: WG960017_B05-W07


title: WG960017_B04-W32


title: WG960017_B07-W12


title: WG960017_B04-W31


title: WG960017_B04-W27


title: WG960017_B04-W21


title: WG960017_B07-W02


title: WG960017_B04-W28


title: WG960017_B04-W26


title: WG960017_B04-W13


title: WG960017_B04-W17


title: WG960017_B04-W11


title: WG960017_B04-W05


title: WG960017_B03-W23


title: WG960017_B03-W19


title: WG960017_B03-W20


title: WG960017_B03-W17


title: WG960017_B03-W22


title: WG960017_B03-W07


title: WG960017_B03-W15


title: WG960017_B03-W03


title: WG960017_B03-W06


title: WG960017_B02-W18


title: WG960017_B02-W17


title: WG960017_B02-W16


title: WG960017_B02-W15


title: WG960017_B02-W12


title: WG960017_B04-W25


title: WG960017_A11-W06


title: WG960017_A10-W25


title: WG960017_B01-W09


title: WG960017_B01-W18


title: WG960017_B02-W03


title: WG960017_A10-W16


title: WG960017_B03-W24


title: WG960017_A09-W11


title: WG960017_A10-W06


title: WG960017_A10-W07


title: WG960017_A10-W12


title: WG960017_A09-W13


title: WG960017_A08-W29


title: WG960017_A08-W22


title: WG960017_A08-W27


title: WG960017_A09-W07


title: WG960017_A08-W17


title: WG960017_A08-W15


title: WG960017_A09-W02


title: WG960017_A08-W14


title: WG960017_A08-W13


title: WG960017_A09-W03


title: WG960017_A08-W08


title: WG960017_A08-W05


title: WG960017_A08-W16


title: WG960017_A07-W31


title: WG960017_A07-W29


title: WG960017_A07-W28


title: WG960017_A07-W27


title: WG960017_A07-W23


title: WG960017_A07-W09


title: WG960017_A07-W13


title: WG960017_A07-W21


title: WG960017_A07-W10


title: WG960017_A07-W08


title: WG960017_A07-W17


title: WG960017_A06-W27


title: WG960017_A08-W06


title: WG960017_A06-W09


title: WG960017_A05-W20


title: WG960017_A06-W10


title: WG960017_A05-W11


title: WG960017_A05-W10


title: WG960017_A05-W15


title: WG960017_A06-W13


title: WG960017_A04-W26


title: WG960017_A04-W17


title: WG960017_A04-W28


title: WG960017_A04-W27


title: WG960017_A04-W05


title: WG960017_A04-W04


title: WG960017_A04-W13


title: WG960017_A03-W30


title: WG960017_A03-W29


title: WG960017_A04-W01


title: WG960017_A03-W15


title: WG960017_A03-W27


title: WG960017_A04-W10


title: WG960017_A03-W12


title: WG960017_A03-W24


title: WG960017_A06-W18


title: WG960017_A02-W26


title: WG960017_A05-W12


title: WG960017_A04-W18


title: WG960017_A02-W23


title: WG960017_A02-W19


title: WG960017_A02-W20


title: WG960017_A02-W17


title: WG960017_A02-W13


title: WG960017_A02-W07


title: WG960017_A01-W10


title: WG960017_A02-W11


title: WG960017_A01-W06


title: WG960016_H06-W15


title: WG960016_H06-W10


title: WG960016_H06-W03


title: WG960016_H06-W06


title: WG960016_H04-W25


title: WG960017_A03-W21


title: WG960017_A03-W23


title: WG960016_H04-W10


title: WG960016_H04-W07


title: WG960016_H03-W18


title: WG960016_H03-W13


title: WG960016_H03-W16


title: WG960016_H03-W15


title: WG960016_H03-W11


title: WG960016_H03-W09


title: WG960017_A02-W12


title: WG960016_H03-W12


title: WG960016_H01-W31


title: WG960016_H02-W19


title: WG960016_H02-W16


title: WG960016_H02-W26


title: WG960016_H06-W13


title: WG960016_H01-W24


title: WG960016_H01-W29


title: WG960016_H01-W25


title: WG960016_H01-W28


title: WG960016_H01-W14


title: WG960016_H01-W17


title: WG960016_H01-W06


title: WG960016_H04-W15


title: WG960016_H01-W09


title: WG960016_H01-W02


title: WG960016_G06-W28


title: WG960016_G06-W27


title: WG960016_G06-W25


title: WG960016_G06-W16


title: WG960016_G06-W13


title: WG960016_G06-W15


title: WG960016_G06-W09


title: WG960016_G06-W06


title: WG960016_G06-W03


title: WG960016_H02-W11


title: WG960016_H01-W27


title: WG960016_G05-W28


title: WG960016_G05-W31


title: WG960016_G05-W20


title: WG960016_H01-W16


title: WG960016_G05-W13


title: WG960016_G05-W17


title: WG960016_G05-W19


title: WG960016_G05-W22


title: WG960016_G05-W08


title: WG960016_G05-W06


title: WG960016_G05-W03


title: WG960016_G05-W01


title: WG960016_G05-W07


title: WG960016_G05-W23


title: WG960016_G04-W13


title: WG960016_G04-W16


title: WG960016_G04-W12


title: WG960016_G04-W08


title: WG960016_G04-W19


title: WG960016_G04-W03


title: WG960016_G03-W32


title: WG960016_G03-W29


title: WG960016_G03-W26


title: WG960016_G03-W28


title: WG960016_G03-W17


title: WG960016_G03-W14


title: WG960016_G03-W10


title: WG960016_G03-W12


title: WG960016_G02-W31


title: WG960016_G02-W22


title: WG960016_G04-W18


title: WG960016_G02-W29


title: WG960016_G02-W20


title: WG960016_G02-W16


title: WG960016_G02-W13


title: WG960016_G01-W20


title: WG960016_G01-W24


title: WG960016_G01-W18


title: WG960016_G03-W31


title: WG960016_G01-W10


title: WG960016_G01-W16


title: WG960016_G01-W13


title: WG960016_G03-W20


title: WG960016_G01-W15


title: WG960016_G01-W12


title: WG960016_G03-W02


title: WG960016_F08-W01


title: WG960016_G01-W05


title: WG960016_F06-W29


title: WG960016_G02-W17


title: WG960016_F06-W18


title: WG960016_F06-W26


title: WG960016_G02-W14


title: WG960016_F06-W14


title: WG960016_G02-W12


title: WG960016_F06-W12


title: WG960016_F06-W10


title: WG960016_F06-W17


title: WG960016_G01-W29


title: WG960016_F05-W17


title: WG960016_F05-W18


title: WG960016_F06-W09


title: WG960016_F05-W16


title: WG960016_G01-W32


title: WG960016_F05-W10


title: WG960016_F05-W09


title: WG960016_F05-W04


title: WG960016_F05-W05


title: WG960016_F05-W03


title: WG960016_F06-W30


title: WG960016_F04-W25


title: WG960016_F04-W18


title: WG960016_F04-W16


title: WG960016_F04-W09


title: WG960016_F04-W08


title: WG960016_F04-W05


title: WG960016_F04-W04


title: WG960016_F04-W10


title: WG960016_F04-W03


title: WG960016_F04-W02


title: WG960016_F03-W29


title: WG960016_F03-W28


title: WG960016_F03-W31


title: WG960016_F03-W25


title: WG960016_F03-W19


title: WG960016_F03-W23


title: WG960016_F03-W18


title: WG960016_F03-W14


title: WG960016_F03-W12


title: WG960016_F03-W08


title: WG960016_F03-W15


title: WG960016_F03-W13


title: WG960016_F03-W06


title: WG960016_F04-W20


title: WG960016_F03-W02


title: WG960016_F03-W01


title: WG960016_F02-W27


title: WG960016_F02-W21


title: WG960016_F02-W23


title: WG960016_F02-W12


title: WG960016_F02-W05


title: WG960016_F02-W06


title: WG960016_F02-W04


title: WG960016_F01-W23


title: WG960016_F01-W22


title: WG960016_F01-W28


title: WG960016_F01-W15


title: WG960016_F01-W14


title: WG960016_F01-W19


title: WG960016_F01-W02


title: WG960016_F01-W16


title: WG960016_F01-W03


title: WG960016_F01-W07


title: WG960016_E06-W10


title: WG960016_E06-W12


title: WG960016_E06-W07


title: WG960016_E06-W15


title: WG960016_E06-W28


title: WG960016_E06-W20


title: WG960016_E06-W04


title: WG960016_E05-W25


title: WG960016_E06-W05


title: WG960016_E06-W03


title: WG960016_E05-W21


title: WG960016_E05-W20


title: WG960016_E06-W11


title: WG960016_E05-W28


title: WG960016_E05-W16


title: WG960016_E05-W13


title: WG960016_E04-W29


title: WG960016_E04-W27


title: WG960016_E04-W22


title: WG960016_E05-W06


title: WG960016_E05-W12


title: WG960016_E05-W11


title: WG960016_E04-W13


title: WG960016_E04-W12


title: WG960016_E04-W10


title: WG960016_E04-W09


title: WG960016_E06-W02


title: WG960016_E04-W05


title: WG960016_E03-W29


title: WG960016_E04-W01


title: WG960016_E03-W32


title: WG960016_E05-W17


title: WG960016_E04-W04


title: WG960016_E03-W28


title: WG960016_E03-W24


title: WG960016_E03-W31


title: WG960016_E03-W23


title: WG960016_E03-W20


title: WG960016_E03-W22


title: WG960016_E03-W13


title: WG960016_E03-W14


title: WG960016_E03-W16


title: WG960016_E03-W11


title: WG960016_E03-W10


title: WG960016_E03-W07


title: WG960016_E03-W06


title: WG960016_E03-W05


title: WG960016_E02-W28


title: WG960016_E02-W21


title: WG960016_E02-W25


title: WG960016_E03-W27


title: WG960016_E02-W20


title: WG960016_E02-W14


title: WG960016_F04-W15


title: WG960016_E02-W17


title: WG960016_E02-W19


title: WG960016_E02-W13


title: WG960016_E02-W09


title: WG960016_E02-W16


title: WG960016_E02-W08


title: WG960016_E02-W03


title: WG960016_E01-W29


title: WG960016_E02-W07


title: WG960016_E02-W11


title: WG960016_E02-W06


title: WG960016_E01-W08


title: WG960016_E02-W05


title: WG960016_E01-W13


title: WG960016_D06-W11


title: WG960016_E01-W15


title: WG960016_D06-W05


title: WG960016_E02-W26


title: WG960016_D05-W18


title: WG960016_D05-W12


title: WG960016_D05-W11


title: WG960016_D05-W06


title: WG960016_D05-W10


title: WG960016_D05-W05


title: WG960016_D05-W03


title: WG960016_D05-W01


title: WG960016_E01-W06


title: WG960016_D04-W25


title: WG960016_D04-W13


title: WG960016_D04-W04


title: WG960016_D06-W14


title: WG960016_D04-W02


title: WG960016_D03-W29


title: WG960016_D04-W09


title: WG960016_D03-W30


title: WG960016_D06-W02


title: WG960016_D03-W27


title: WG960016_D03-W21


title: WG960016_D03-W19


title: WG960016_D03-W17


title: WG960016_D03-W22


title: WG960016_D03-W23


title: WG960016_D03-W20


title: WG960016_D03-W08


title: WG960016_D03-W04


title: WG960016_D01-W31


title: WG960016_D03-W02


title: WG960016_D01-W32


title: WG960016_D01-W30


title: WG960016_D01-W29


title: WG960016_D01-W28


title: WG960016_D01-W19


title: WG960016_D01-W24


title: WG960016_D01-W16


title: WG960016_D01-W13


title: WG960016_D01-W14


title: WG960016_D01-W04


title: WG960016_D01-W11


title: WG960016_D01-W02


title: WG960016_D01-W09


title: WG960016_C06-W30


title: WG960016_C06-W28


title: WG960016_C06-W26


title: WG960016_C06-W27


title: WG960016_C06-W16


title: WG960016_C06-W11


title: WG960016_C06-W03


title: WG960016_C06-W01


title: WG960016_C05-W20


title: WG960016_C06-W17


title: WG960016_C06-W14


title: WG960016_C05-W19


title: WG960016_C06-W10


title: WG960016_C05-W09


title: WG960016_C05-W10


title: WG960016_C05-W15


title: WG960016_C04-W25


title: WG960016_C04-W16


title: WG960016_C04-W13


title: WG960016_C04-W10


title: WG960016_C04-W09


title: WG960016_D01-W01


title: WG960016_D01-W25


title: WG960016_C04-W04


title: WG960016_C04-W02


title: WG960016_C03-W30


title: WG960016_C04-W01


title: WG960016_C04-W07


title: WG960016_C03-W29


title: WG960016_C03-W21


title: WG960016_C03-W12


title: WG960016_C03-W14


title: WG960016_C03-W19


title: WG960016_C02-W27


title: WG960016_C04-W17


title: WG960016_C03-W09


title: WG960016_C03-W07


title: WG960016_C03-W08


title: WG960016_C02-W21


title: WG960016_C02-W17


title: WG960016_C03-W03


title: WG960016_C02-W11


title: WG960016_C02-W25


title: WG960016_C02-W09


title: WG960016_C02-W10


title: WG960016_C02-W05


title: WG960016_C02-W03


title: WG960016_C02-W07


title: WG960016_C01-W28


title: WG960016_C01-W22


title: WG960016_C03-W10


title: WG960016_C01-W20


title: WG960016_C01-W23


title: WG960016_C01-W24


title: WG960016_C01-W17


title: WG960016_C01-W14


title: WG960016_C02-W22


title: WG960016_C01-W13


title: WG960016_C01-W07


title: WG960016_C01-W11


title: WG960016_C01-W03


title: WG960016_B06-W27


title: WG960016_B06-W18


title: WG960016_C01-W02


title: WG960016_B06-W28


title: WG960016_B06-W10


title: WG960016_B06-W07


title: WG960016_B06-W04


title: WG960016_B06-W14


title: WG960016_C02-W01


title: WG960016_B05-W17


title: WG960016_B05-W26


title: WG960016_B06-W01


title: WG960016_C01-W27


title: WG960016_B05-W16


title: WG960016_B05-W15


title: WG960016_B05-W14


title: WG960016_B05-W06


title: WG960016_B05-W12


title: WG960016_B05-W13


title: WG960016_C01-W04


title: WG960016_B05-W01


title: WG960016_B05-W03


title: WG960016_B04-W18


title: WG960016_B04-W31


title: WG960016_B06-W29


title: WG960016_B04-W10


title: WG960016_B04-W11


title: WG960016_B04-W01


title: WG960016_B03-W18


title: WG960016_B03-W11


title: WG960016_B03-W28


title: WG960016_B04-W06


title: WG960016_B03-W19


title: WG960016_B03-W10


title: WG960016_B06-W02


title: WG960016_B03-W08


title: WG960016_B03-W07


title: WG960016_B03-W03


title: WG960016_B03-W09


title: WG960016_B05-W07


title: WG960016_B03-W15


title: WG960016_B03-W04


title: WG960016_B02-W26


title: WG960016_B02-W28


title: WG960016_B02-W13


title: WG960016_B02-W22


title: WG960016_B02-W14


title: WG960016_B02-W01


title: WG960016_B02-W04


title: WG960016_B02-W07


title: WG960016_B04-W02


title: WG960016_B02-W10


title: WG960016_B01-W31


title: WG960016_B01-W28


title: WG960016_B01-W30


title: WG960016_B01-W26


title: WG960016_B01-W24


title: WG960016_B01-W19


title: WG960016_B01-W22


title: WG960016_B01-W14


title: WG960016_B01-W12


title: WG960016_B01-W10


title: WG960016_B01-W07


title: WG960016_B01-W02


title: WG960016_B01-W06


title: WG960016_B01-W20


title: WG960016_B01-W03


title: WG960016_A06-W26


title: WG960016_A06-W20


title: WG960016_A06-W03


title: WG960016_A06-W08


title: WG960016_A06-W02


title: WG960016_A06-W01


title: WG960016_A05-W11


title: WG960016_A05-W27


title: WG960016_B01-W21


title: WG960016_A05-W06


title: WG960016_B01-W17


title: WG960016_A06-W22


title: WG960016_B01-W15


title: WG960016_A04-W30


title: WG960016_A05-W26


title: WG960016_A04-W05


title: WG960016_A04-W31


title: WG960016_B01-W13


title: WG960016_A04-W21


title: WG960016_A03-W29


title: WG960016_A04-W06


title: WG960016_A04-W14


title: WG960016_A04-W20


title: WG960016_A03-W28


title: WG960016_A03-W22


title: WG960016_A03-W16


title: WG960016_A02-W30


title: WG960016_A02-W31


title: WG960016_A02-W29


title: WG960016_A02-W26


title: WG960016_A06-W21


title: WG960016_A02-W27


title: WG960016_A06-W05


title: WG960016_A03-W21


title: WG960016_A03-W07


title: WG960016_A03-W30


title: WG960016_A06-W14


title: WG960016_A05-W03


title: WG960016_A02-W18


title: WG960016_A02-W14


title: WG960016_A02-W16


title: WG960016_A02-W12


title: WG960016_A02-W19


title: WG960016_A02-W22


title: WG960016_A02-W10


title: WG960016_A02-W11


title: WG960016_A04-W13


title: WG960016_B01-W29


title: WG960016_A02-W09


title: WG960016_A02-W01


title: WG960016_A02-W08


title: WG960016_A02-W07


title: WG960016_A01-W20


title: WG960016_A01-W22


title: WG960016_A01-W25


title: WG960016_A01-W30


title: WG960016_A01-W21


title: WG960016_A01-W26


title: WG960016_A01-W17


title: WG960016_A01-W28


title: WG960016_A01-W15


title: WG960016_A02-W13


title: WG960016_A01-W11


title: WG960016_A01-W08


title: WG960016_A01-W12


title: WG960016_A01-W13


title: WG960016_A01-W07


title: WG960016_A05-W02


title: WG960016_A01-W06


title: WG960015_H12-W04


title: WG960015_H11-W14


title: WG960016_A01-W05


title: WG960015_H11-W05


title: WG960015_H09-W15


title: WG960015_H09-W06


title: WG960015_H09-W05


title: WG960015_H11-W04


title: WG960015_H09-W10


title: WG960016_A01-W03


title: WG960015_G12-W02


title: WG960015_G12-W27


title: WG960015_G12-W05


title: WG960015_H09-W02


title: WG960015_G12-W12


title: WG960015_G12-W17


title: WG960015_G12-W19


title: WG960015_G11-W16


title: WG960015_G10-W22


title: WG960015_G10-W11


title: WG960015_G11-W07


title: WG960015_G10-W14


title: WG960015_G09-W17


title: WG960015_G09-W22


title: WG960015_G09-W31


title: WG960015_G09-W14


title: WG960015_G08-W03


title: WG960015_F12-W09


title: WG960015_G09-W27


title: WG960015_G09-W13


title: WG960015_F12-W04


title: WG960015_G10-W06


title: WG960015_F12-W02


title: WG960015_F12-W07


title: WG960015_F09-W17


title: WG960015_F10-W05


title: WG960015_G11-W21


title: WG960015_F09-W08


title: WG960015_F09-W11


title: WG960015_F09-W07


title: WG960015_F09-W06


title: WG960015_F08-W16


title: WG960015_F08-W07


title: WG960015_F09-W02


title: WG960015_F08-W15


title: WG960015_F08-W03


title: WG960015_F08-W06


title: WG960015_F08-W02


title: WG960015_F08-W01


title: WG960015_E12-W17


title: WG960015_E12-W18


title: WG960015_E12-W11


title: WG960015_F12-W05


title: WG960015_E12-W09


title: WG960015_E11-W14


title: WG960015_E11-W12


title: WG960015_E11-W07


title: WG960015_E12-W04


title: WG960015_E11-W06


title: WG960015_G11-W29


title: WG960015_F09-W15


title: WG960015_E11-W05


title: WG960015_E11-W02


title: WG960015_E11-W04


title: WG960015_E10-W08


title: WG960015_E10-W07


title: WG960015_E10-W04


title: WG960015_E09-W31


title: WG960015_E09-W19


title: WG960015_E09-W13


title: WG960015_E09-W29


title: WG960015_E09-W12


title: WG960015_E09-W11


title: WG960015_E09-W04


title: WG960015_E09-W10


title: WG960015_E09-W01


title: WG960015_E09-W03


title: WG960015_D12-W27


title: WG960015_D12-W25


title: WG960015_E09-W02


title: WG960015_D12-W15


title: WG960015_D11-W16


title: WG960015_D10-W06


title: WG960015_D09-W14


title: WG960015_D09-W12


title: WG960015_D09-W10


title: WG960015_D09-W01


title: WG960015_D09-W03


title: WG960015_D08-W05


title: WG960015_D09-W08


title: WG960015_D09-W09


title: WG960015_E09-W17


title: WG960015_E09-W21


title: WG960015_D08-W01


title: WG960015_C12-W23


title: WG960015_C12-W17


title: WG960015_C12-W09


title: WG960015_C10-W26


title: WG960015_E09-W27


title: WG960015_C10-W15


title: WG960015_C09-W31


title: WG960015_C11-W12


title: WG960015_D11-W12


title: WG960015_C09-W27


title: WG960015_C09-W29


title: WG960015_C09-W18


title: WG960015_C09-W23


title: WG960015_C09-W16


title: WG960015_D08-W25


title: WG960015_C12-W14


title: WG960015_C09-W17


title: WG960015_C09-W15


title: WG960015_C09-W03


title: WG960015_C12-W22


title: WG960015_C08-W09


title: WG960015_C08-W02


title: WG960015_B12-W14


title: WG960015_C08-W05


title: WG960015_C08-W01


title: WG960015_B10-W04


title: WG960015_B10-W07


title: WG960015_B12-W12


title: WG960015_B11-W25


title: WG960015_B12-W07


title: WG960015_B11-W08


title: WG960015_B12-W03


title: WG960015_B10-W01


title: WG960015_B10-W10


title: WG960015_B09-W25


title: WG960015_B09-W22


title: WG960015_B08-W30


title: WG960015_B09-W21


title: WG960015_B08-W26


title: WG960015_B08-W19


title: WG960015_B08-W05


title: WG960015_B08-W17


title: WG960015_C08-W03


title: WG960015_B08-W09


title: WG960015_B08-W14


title: WG960015_C12-W04


title: WG960015_A12-W16


title: WG960015_B08-W03


title: WG960015_B08-W02


title: WG960015_C09-W24


title: WG960015_A11-W12


title: WG960015_A10-W14


title: WG960015_A11-W15


title: WG960015_A11-W07


title: WG960015_A10-W05


title: WG960015_A10-W13


title: WG960015_A09-W32


title: WG960015_A09-W27


title: WG960015_A09-W18


title: WG960015_A09-W25


title: WG960015_A09-W26


title: WG960015_A09-W16


title: WG960015_A09-W17


title: WG960015_A09-W30


title: WG960015_B08-W27


title: WG960015_A09-W08


title: WG960015_A09-W13


title: WG960015_A09-W02


title: WG960015_A09-W05


title: WG960011_H12-W28


title: WG960011_H11-W13


title: WG960015_A08-W01


title: WG960011_H11-W05


title: WG960011_H10-W28


title: WG960011_H12-W16


title: WG960011_H12-W18


title: WG960011_H10-W18


title: WG960011_H10-W16


title: WG960011_H11-W09


title: WG960011_H10-W21


title: WG960011_H10-W12


title: WG960011_H10-W05


title: WG960011_H10-W01


title: WG960011_H09-W06


title: WG960011_H08-W17


title: WG960015_A09-W10


title: WG960011_H08-W18


title: WG960011_H08-W16


title: WG960011_H08-W11


title: WG960011_H08-W10


title: WG960011_H08-W08


title: WG960011_H07-W04


title: WG960011_H08-W05


title: WG960011_H07-W01


title: WG960011_H06-W25


title: WG960011_H06-W11


title: WG960011_H04-W28


title: WG960011_H06-W03


title: S2IP2

city: Madison

first: Philip

last: Anderson

state: WI

address: 425-G Henry Mall

department: Genetics

institute: University of Wisconsin-Madison

description:
polyA-selected RNA
SMG-2 Immunoprecipitation (RIP-Seq)

data_processing:
Illumina Casava1.8.2 software used for basecalling.
Sequenced reads were trimmed for adaptor sequences
Reads were subjected to an additional quality trim using trimmomatic 0.32 and the following requirements: LEADING:10 TRAILING:10 SLIDINGWINDOW:4:20 MINLEN:25
Reads mapped to WBcel235 using tophat  v2.0.12 with parameters  -p 6 -i 10 -I 21230 -G [using GTF from iGenomes for WBcel235] (Used with bowtie v. 2.2.3.0)
Aligned reads counted by gene (using iGenomes GTF) and intron (using GTFs with introns and exons collapsed across all transcripts) using htseq-count
All read counts subjected to normalization and DE analysis using EdgeR
Genome_build: Ensembl WBcel235
Supplementary_files_format_and_content: tab-delimited text files include raw count values for each gene or intron (using IntronMergeGTF.gtf) for each sample.
Supplementary_files_format_and_content: IntronMergeGTF.gtf: GTF annotating introns after collapsing a given gene's transcripts (overlapping exons are merged so that final intron definition selects regions which are annotated as introns in all available transcripts).

organism: Caenorhabditis elegans

growth_protocol: We generated synchronized strains by treating gravid adults with a 5.25% hypochlorite solution.  Embryos were grown to L4 stage in S-media at 20°C.

extract_protocol:
RNA was extracted from IP pellets and inputs using TRIzol (Life Technologies).  Input samples were DNase treated.  IP samples showed no gDNA contamination via PCR and were not subjected to DNase treatment.  PolyA selection and cDNA library construction using the non-strand-specific Illumina TruSeq kit was performed by the University of Wisconsin Biotechnology Center DNA Sequence Facility.  Approximately 500ng of RNA was used for input sample library preparation; all of the submitted RNA (45-228ng per negative control IP sample, 263-945ng per experimental IP) was used for IP sample library preparation.
RNA libraries were prepared for sequencing using standard Illumina protocols

treatment_protocol: Staged L4 animals were frozen in immunoprecipitation (IP) lysis buffer (20mM MOPS pH 7.2, 100mM NaCl, 0.01% NP-40, protease inhibitor cocktail (Sigma), pepstatin A (Sigma), RNase OUT, and PMSF).  Cells were lysed by sonication with 7x5s pulses, centrifuged at 14,000 x g for 25 min, and the supernatant fraction was retained.  Bradford assays were used to determine the extract concentration.  Extracts were diluted to 2mg/mL in IP lysis buffer.  One aliquot of this total RNA was preserved as the input sample while a second aliquot was incubated with SMG-2 antibody (previously described [23]) coupled to Dynabead Protein G (Life Technologies) for the IP sample.  Precipitated proteins were collected on the magnet and washed six times with IP wash buffer (20mM MOPS pH 7.2, 100mM NaCl, 0.02% NP-40).

tag: developmental stage

tag: antibody


title: S2IP3


title: S2In2

description:
gDNA-reduced polyA-selected RNA
input RNAseq sample


title: S2IP1


title: S2In1


title: S2In3


title: S1IP2


title: WG960011_H09-W17


title: S1IP1


title: S1IP3


title: WG960011_H08-W14


title: WG960011_H08-W15


title: S1In3

source_name: smg-1(r910) Input 3


title: S1In1

source_name: smg-1(r910) Input 1


title: WG960011_H04-W19


title: N2In2

source_name: N2 Input 2


title: N2In3

source_name: N2 Input 3


title: S1In2

source_name: smg-1(r910) Input 2


title: WG960011_H06-W18


title: WG960011_H04-W15


title: WG960011_H04-W09


title: WG960011_H04-W08


title: WG960011_H03-W18


title: WG960011_H04-W01


title: WG960011_H06-W06


title: WG960011_H06-W01


title: WG960011_H03-W13


title: WG960011_H03-W07


title: WG960011_H03-W04


title: WG960011_H02-W14


title: WG960011_H03-W01


title: WG960011_H02-W08


title: WG960011_H01-W20


title: WG960011_H01-W21


title: WG960011_H02-W07


title: WG960011_H02-W05


title: N2In1

source_name: N2 Input 1


title: WG960011_H01-W14


title: WG960011_H01-W04


title: WG960011_G12-W12


title: WG960011_H01-W11


title: WG960011_H01-W10


title: WG960011_G12-W03


title: WG960011_G11-W15


title: WG960011_G11-W03


title: WG960011_G11-W11


title: WG960011_G12-W10


title: WG960011_G12-W08


title: WG960011_G09-W20


title: WG960011_H01-W05


title: WG960011_G08-W29


title: WG960011_G09-W07


title: WG960011_G11-W14


title: WG960011_G08-W27


title: WG960011_G08-W22


title: WG960011_G08-W16


title: WG960011_G08-W10


title: WG960011_G08-W07


title: WG960011_G08-W02


title: WG960011_G08-W06


title: WG960011_G06-W17


title: WG960011_G06-W25


title: WG960011_G06-W11


title: WG960011_G08-W01


title: WG960011_G05-W15


title: WG960011_G06-W16


title: WG960011_G06-W01


title: WG960011_G06-W07


title: WG960011_G05-W04


title: WG960011_G05-W05


title: WG960011_G04-W18


title: WG960011_G05-W10


title: WG960011_G05-W12


title: WG960011_G09-W14


title: WG960011_G04-W05


title: WG960011_G08-W12


title: WG960011_G03-W16


title: WG960011_G08-W13


title: WG960011_G11-W10


title: WG960011_G03-W10


title: WG960011_G04-W15


title: WG960011_G03-W04


title: WG960011_G03-W12


title: WG960011_G03-W11


title: WG960011_G03-W03


title: WG960011_G02-W26


title: WG960011_G03-W01


title: WG960011_G02-W20


title: WG960011_G06-W05


title: WG960011_G02-W17


title: WG960011_G05-W03


title: WG960011_G02-W11


title: WG960011_G02-W15


title: WG960011_G01-W28


title: WG960011_F12-W06


title: WG960011_G03-W09


title: WG960011_F12-W17


title: WG960011_G01-W08


title: WG960011_F12-W01


title: WG960011_F12-W14


title: WG960011_F12-W05


title: WG960011_F11-W02


title: WG960011_F12-W02


title: WG960011_F10-W06


title: WG960011_F11-W09


title: WG960011_F09-W29


title: WG960011_F10-W07


title: WG960011_G02-W29


title: WG960011_F08-W25


title: WG960011_G02-W22


title: WG960011_F09-W17


title: WG960011_F09-W05


title: WG960011_G02-W16


title: WG960011_F08-W06


title: WG960011_F08-W02


title: WG960011_G02-W10


title: WG960011_F07-W19


title: WG960011_F08-W03


title: WG960011_F06-W28


title: WG960011_F07-W01


title: WG960011_F07-W08


title: WG960011_F06-W06


title: WG960011_F05-W25


title: WG960011_F05-W15


title: WG960011_F05-W16


title: WG960011_F05-W17


title: WG960011_F05-W13


title: WG960011_F05-W07


title: WG960011_F05-W18


title: WG960011_F05-W02


title: WG960011_F06-W15


title: WG960011_F04-W26


title: WG960011_F07-W11


title: WG960011_F08-W11


title: WG960011_F04-W14


title: WG960011_F04-W08


title: WG960011_F04-W09


title: WG960011_F04-W18


title: WG960011_F04-W05


title: WG960011_F04-W02


title: WG960011_F04-W04


title: WG960011_F07-W16


title: WG960011_F04-W07


title: WG960011_F04-W13


title: WG960011_F03-W12


title: WG960011_F03-W02


title: WG960011_F02-W27


title: WG960011_F02-W07


title: WG960011_F01-W12


title: WG960011_F01-W25


title: WG960011_F02-W08


title: WG960011_E12-W31


title: WG960011_F01-W07


title: WG960011_E12-W20


title: WG960011_E12-W06


title: WG960011_E12-W07


title: WG960011_E12-W13


title: WG960011_E12-W14


title: WG960011_E12-W22


title: WG960011_E12-W03


title: WG960011_E10-W25


title: WG960011_E12-W02


title: WG960011_E10-W16


title: WG960011_E10-W12


title: WG960011_E09-W15


title: WG960011_E10-W18


title: WG960011_E10-W27


title: WG960011_E08-W22


title: WG960011_E09-W03


title: WG960011_E09-W01


title: WG960011_E08-W16


title: WG960011_E08-W25


title: WG960011_E08-W11


title: WG960011_E08-W31


title: WG960011_E08-W05


title: WG960011_E06-W27


title: WG960011_E08-W19


title: WG960011_E12-W21


title: WG960011_E08-W15


title: WG960011_E06-W25


title: WG960011_E04-W28


title: WG960011_E06-W13


title: WG960011_E04-W19


title: WG960011_E04-W03


title: WG960011_E04-W18


title: WG960011_E04-W13


title: WG960011_E02-W27


title: WG960011_E09-W06


title: WG960011_D12-W29


title: WG960011_E02-W21


title: WG960011_E02-W11


title: WG960011_D12-W12


title: WG960011_D11-W08


title: WG960011_D11-W05


title: WG960011_D10-W28


title: WG960011_D11-W13


title: WG960011_E08-W06


title: WG960011_D10-W18


title: WG960011_D10-W25


title: WG960011_D10-W11


title: WG960011_D10-W01


title: WG960011_D10-W02


title: WG960011_D08-W31


title: WG960011_D10-W03


title: WG960011_D09-W08


title: WG960011_D09-W06


title: WG960011_D08-W30


title: WG960011_D08-W23


title: WG960011_D08-W22


title: WG960011_D10-W06


title: WG960011_D08-W16


title: WG960011_D08-W15


title: WG960011_D08-W04


title: WG960011_D08-W14


title: WG960011_D07-W13


title: WG960011_D06-W26


title: WG960011_D06-W19


title: WG960011_D06-W11


title: WG960011_D06-W13


title: WG960011_D07-W02


title: WG960011_D06-W10


title: WG960011_D06-W04


title: WG960011_D09-W16


title: WG960011_D05-W14


title: WG960011_D05-W13


title: WG960011_D06-W01


title: WG960011_D05-W08


title: WG960011_D05-W06


title: WG960011_D05-W03


title: WG960011_D08-W18


title: WG960011_D05-W01


title: WG960011_D04-W27


title: WG960011_D04-W26


title: WG960011_D04-W19


title: WG960011_D04-W09


title: WG960011_D04-W08


title: WG960011_D04-W15


title: WG960011_D04-W04


title: WG960011_D07-W09


title: WG960011_D04-W05


title: WG960011_D07-W11


title: WG960011_D04-W07


title: WG960011_D03-W31


title: WG960011_D03-W32


title: WG960011_D03-W20


title: WG960011_D10-W05


title: WG960011_D03-W21


title: WG960011_D03-W19


title: WG960011_D03-W26


title: WG960011_D03-W25


title: WG960011_D03-W13


title: WG960011_D03-W12


title: WG960011_D03-W14


title: WG960011_D03-W11


title: WG960011_D03-W03


title: WG960011_D03-W07


title: WG960011_D03-W01


title: WG960011_D02-W26


title: WG960011_D02-W16


title: WG960011_D02-W11


title: WG960011_D02-W10


title: WG960011_D02-W06


title: WG960011_D02-W02


title: WG960011_D01-W15


title: WG960011_D01-W14


title: WG960011_D01-W10


title: WG960011_D01-W02


title: WG960011_D01-W05


title: WG960011_C12-W27


title: WG960011_C12-W25


title: WG960011_D01-W09


title: WG960011_D01-W11


title: WG960011_C12-W03


title: WG960011_C11-W11


title: WG960011_C11-W16


title: WG960011_C12-W08


title: WG960011_C12-W13


title: WG960011_C11-W03


title: WG960011_C11-W02


title: WG960011_C10-W03


title: WG960011_C11-W01


title: WG960011_C10-W16


title: WG960011_C09-W06


title: WG960011_C10-W18


title: WG960011_C08-W29


title: WG960011_C10-W11


title: WG960011_C09-W01


title: WG960011_C09-W19


title: WG960011_C08-W27


title: WG960011_C10-W05


title: WG960011_C08-W15


title: WG960011_C08-W02


title: WG960011_C08-W01


title: WG960011_C07-W07


title: WG960011_C07-W03


title: WG960011_C08-W10


title: WG960011_C06-W27


title: WG960011_D02-W01


title: WG960011_C09-W10


title: WG960011_C08-W12


title: WG960011_C08-W14


title: WG960011_C06-W12


title: WG960011_C08-W11


title: WG960011_C06-W13


title: WG960011_C08-W03


title: WG960011_C06-W10


title: WG960011_C06-W19


title: WG960011_C06-W05


title: WG960011_C08-W30


title: WG960011_C05-W10


title: WG960011_C06-W04


title: WG960011_C05-W04


title: WG960011_C05-W09


title: WG960011_C05-W01


title: WG960011_C04-W19


title: WG960011_C06-W11


title: WG960011_C07-W04


title: WG960011_C06-W17


title: WG960011_C05-W12


title: WG960011_C04-W16


title: WG960011_C04-W09


title: WG960011_C04-W11


title: WG960011_C04-W05


title: WG960011_C03-W26


title: WG960011_C03-W16


title: WG960011_C03-W11


title: WG960011_C02-W19


title: WG960011_C03-W03


title: WG960011_C02-W17


title: WG960011_C03-W04


title: WG960011_C05-W11


title: WG960011_C02-W09


title: WG960011_C02-W16


title: WG960011_C02-W07


title: WG960011_C05-W05


title: WG960011_C01-W27


title: WG960011_C01-W21


title: WG960011_C01-W19


title: WG960011_C01-W22


title: WG960011_C01-W14


title: WG960011_C04-W02


title: WG960011_C01-W01


title: WG960011_B12-W31


title: WG960011_C01-W09


title: WG960011_B12-W25


title: WG960011_B12-W13


title: WG960011_C03-W02


title: WG960011_B12-W11


title: WG960011_B10-W16


title: WG960011_B12-W12


title: WG960011_B12-W18


title: WG960011_B10-W07


title: WG960011_B09-W28


title: WG960011_B10-W12


title: WG960011_B09-W29


title: WG960011_B09-W20


title: WG960011_B09-W16


title: WG960011_B09-W19


title: WG960011_B09-W18


title: WG960011_B09-W17


title: WG960011_C01-W30


title: WG960011_C01-W18


title: WG960011_C01-W06


title: WG960011_B09-W12


title: WG960011_B09-W05


title: WG960011_B08-W18


title: WG960011_B09-W03


title: WG960011_B08-W10


title: WG960011_B08-W16


title: WG960011_B07-W16


title: WG960011_B08-W06


title: WG960011_B08-W11


title: WG960011_B07-W07


title: WG960011_B07-W06


title: WG960011_B06-W10


title: WG960011_B06-W06


title: WG960011_B06-W17


title: WG960011_B05-W13


title: WG960011_B05-W06


title: WG960011_B05-W05


title: WG960011_B05-W01


title: WG960011_B06-W04


title: WG960011_B04-W19


title: WG960011_B05-W02


title: WG960011_B04-W10


title: WG960011_B04-W06


title: WG960011_B04-W02


title: WG960011_B03-W31


title: WG960011_B03-W28


title: WG960011_B07-W11


title: WG960011_C01-W29


title: WG960011_B03-W20


title: WG960011_C01-W11


title: WG960011_B03-W19


title: WG960011_B02-W30


title: WG960011_B03-W05


title: WG960011_B02-W20


title: WG960011_B03-W11


title: WG960011_B05-W11


title: WG960011_B02-W25


title: WG960011_B03-W13


title: WG960011_B04-W17


title: WG960011_B04-W13


title: WG960011_B02-W10


title: WG960011_B02-W16


title: WG960011_B02-W11


title: WG960011_B02-W04


title: WG960011_B02-W06


title: WG960011_B02-W03


title: WG960011_B01-W15


title: WG960011_B02-W02


title: WG960011_B03-W25


title: WG960011_B02-W01


title: WG960011_B01-W12


title: WG960011_B01-W14


title: WG960011_B01-W09


title: WG960011_A12-W27


title: WG960011_B03-W15


title: WG960011_A12-W22


title: WG960011_A12-W26


title: WG960011_A12-W21


title: WG960011_B02-W17


title: WG960011_A10-W17


title: WG960011_A10-W25


title: WG960011_A10-W13


title: WG960011_A10-W08


title: WG960011_A10-W01


title: WG960011_B02-W07


title: WG960011_A10-W11


title: WG960011_A09-W20


title: WG960011_A09-W09


title: WG960011_A09-W04


title: WG960011_A09-W08


title: WG960011_A08-W12


title: WG960011_A08-W15


title: WG960011_B01-W13


title: WG960011_A08-W08


title: WG960011_A08-W28


title: WG960011_A08-W03


title: WG960011_A08-W06


title: WG960011_A06-W26


title: WG960011_A08-W01


title: WG960011_A06-W18


title: WG960011_A06-W25


title: WG960011_A06-W10


title: WG960011_A06-W15


title: WG960011_A06-W02


title: WG960011_A12-W03


title: WG960011_A06-W28


title: WG960011_A04-W27


title: WG960011_A04-W18


title: WG960011_A04-W15


title: WG960011_A04-W10


title: WG960011_A02-W17


title: WG960011_A04-W02


title: WG960011_A02-W15


title: WG960011_A02-W13


title: WG960011_A02-W12


title: WG960011_A02-W09


title: WG960011_A02-W01


title: WG960011_A08-W29


title: PTBP1 iCLIP replicate 5

first: Felix

last: Krueger

address: Granta Park

department: Bioinformatics

country: United Kingdom

institute: Altos Labs

description: Splenic B cells isolated by negative MACS sorting were stimulated ex vivo with LPS for 48h. 5' Barcode used for iCLIP demultiplexing and deduplication CTTNNNN

data_processing:
Library strategy: iClip
Reads were demultiplexed using the first three known 5' nucleotides
Reads were mapped to the using Bowtie
Reads were deduplicated based on their random 5' barcode, see Konig et al. for all details (Nature Structural & Molecular Biology 17, 909–915 (2010)
Position -1 of deduplicated reads was annotated as the cross-link site,  see Konig et al. for all details (Nature Structural & Molecular Biology 17, 909–915 (2010)
Peak calling was carried out as described in Konig et al (Nature Structural & Molecular Biology 17, 909–915 (2010)) for individual replicates and also for all the replicates together
Bed files contain the possition of the significant cross-link site (FDR<0.05) and the number of individual cross-link sites found for that particular site with the strand information. There are Bed files for the individual replicates and for all the replicates pooled together.
Genome_build: GRCm38

source_name: Splenic B cells, LPS 48h

extract_protocol:
30*10^6 cells were used in each immunoprecipitation and were lysed in 50 mM Tris-HCL pH7.4, 100mM NaCl, 1% NP-40 and 0.1% SDS lysis buffer.
See König et al, 2010 Nature Structural and Molecular Biology, 17, 909-915. Individual-nucleotide resolution UV crosslinking and immunoprecipitation (iCLIP). Samples were multiplexed based on a 7 bases barcode (annotated under sample description)

tag: Sex

value: Female

tag: day of sort

value: Splenic B cells


title: PTBP1 iCLIP replicate 3

description: Splenic B cells isolated by negative MACS sorting were stimulated ex vivo with LPS for 48h. 5' Barcode used for iCLIP demultiplexing and deduplication CCCNNNN


title: PTBP1 iCLIP replicate 4

description: Splenic B cells isolated by negative MACS sorting were stimulated ex vivo with LPS for 48h. 5' Barcode used for iCLIP demultiplexing and deduplication TCTNNNN


title: PTBP1 iCLIP replicate 2

description: Splenic B cells isolated by negative MACS sorting were stimulated ex vivo with LPS for 48h. 5' Barcode used for iCLIP demultiplexing and deduplication AGTNNNN


title: PTBP1 iCLIP replicate 1

description: Splenic B cells isolated by negative MACS sorting were stimulated ex vivo with LPS for 48h. 5' Barcode used for iCLIP demultiplexing and deduplication GGCNNNN


title: Lung single cell 1502

city: Uppsala

first: L.

last: He

address: Dag Hammarskjölds Väg 20

institute: Uppsala University

description: lung single cell 1502

data_processing:
RNA-seq reads were mapped to the mouse reference genome (mm10) using STAR (version STAR_2.4.2a) and the count matrix shows the individual counts aligning to each gene per cell. In order to compare the gene expression counts between different cells, the total gene counts for each cell was normalized to 500,000.
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include raw and normalized counts values for each sample

source_name: Lung

extract_protocol:
We used different transgenic mouse reporter lines with fluorescent expression in different populations of vascular cells, Cldn5(BAC)eGFP mice that strongly labels lung endothelial cells, mice positive for Pdgfrb(BAC)eGFP and NG2dsRed.  We chose to focus our efforts to adult mice 12 to 15 weeks old in order to primarily define fully differentiated and quiescent vascular cells and we chose a methodology based on fluorescence-activated cell sorting (FACS) into 384-well plates followed by the SmartSeq2 methodology for the generation and sequencing of full length polyadenylated cDNAs
Libraries for RNA-seq were prepared by using SMART-seq2 methodology

value: lung

value: adult mice 12 to 15 weeks old

value: Pdgfrb(BAC)eGFP


title: Lung single cell 1501

description: lung single cell 1501


title: Lung single cell 1503

description: lung single cell 1503


title: Lung single cell 1499

description: lung single cell 1499


title: Lung single cell 1494

description: lung single cell 1494


title: Lung single cell 1496

description: lung single cell 1496


title: Lung single cell 1500

description: lung single cell 1500


title: Lung single cell 1504

description: lung single cell 1504


title: Lung single cell 1491

description: lung single cell 1491


title: Lung single cell 1498

description: lung single cell 1498


title: Lung single cell 1490

description: lung single cell 1490


title: Lung single cell 1495

description: lung single cell 1495


title: WG960011_A04-W17

value: somatosensory cortex


title: Lung single cell 1492

description: lung single cell 1492


title: Lung single cell 1485

description: lung single cell 1485


title: Lung single cell 1486

description: lung single cell 1486


title: Lung single cell 1487

description: lung single cell 1487


title: Lung single cell 1488

description: lung single cell 1488


title: Lung single cell 1484

description: lung single cell 1484


title: Lung single cell 1483

description: lung single cell 1483


title: Lung single cell 1481

description: lung single cell 1481


title: Lung single cell 1482

description: lung single cell 1482


title: Lung single cell 1480

description: lung single cell 1480


title: Lung single cell 1477

description: lung single cell 1477


title: Lung single cell 1479

description: lung single cell 1479


title: Lung single cell 1476

description: lung single cell 1476


title: Lung single cell 1497

description: lung single cell 1497


title: Lung single cell 1475

description: lung single cell 1475


title: Lung single cell 1472

description: lung single cell 1472


title: Lung single cell 1473

description: lung single cell 1473


title: Lung single cell 1493

description: lung single cell 1493


title: Lung single cell 1489

description: lung single cell 1489


title: Lung single cell 1470

description: lung single cell 1470


title: Lung single cell 1471

description: lung single cell 1471


title: Lung single cell 1464

description: lung single cell 1464


title: Lung single cell 1469

description: lung single cell 1469


title: Lung single cell 1468

description: lung single cell 1468


title: Lung single cell 1465

description: lung single cell 1465


title: Lung single cell 1466

description: lung single cell 1466


title: Lung single cell 1474

description: lung single cell 1474


title: Lung single cell 1463

description: lung single cell 1463


title: Lung single cell 1467

description: lung single cell 1467


title: Lung single cell 1462

description: lung single cell 1462


title: Lung single cell 1461

description: lung single cell 1461


title: Lung single cell 1455

description: lung single cell 1455


title: Lung single cell 1457

description: lung single cell 1457


title: Lung single cell 1478

description: lung single cell 1478


title: Lung single cell 1460

description: lung single cell 1460


title: Lung single cell 1459

description: lung single cell 1459


title: Lung single cell 1456

description: lung single cell 1456


title: Lung single cell 1452

description: lung single cell 1452


title: Lung single cell 1453

description: lung single cell 1453


title: Lung single cell 1449

description: lung single cell 1449


title: Lung single cell 1458

description: lung single cell 1458


title: Lung single cell 1454

description: lung single cell 1454


title: Lung single cell 1448

description: lung single cell 1448


title: Lung single cell 1451

description: lung single cell 1451


title: Lung single cell 1443

description: lung single cell 1443


title: Lung single cell 1446

description: lung single cell 1446


title: Lung single cell 1450

description: lung single cell 1450


title: Lung single cell 1447

description: lung single cell 1447


title: Lung single cell 1445

description: lung single cell 1445


title: Lung single cell 1438

description: lung single cell 1438


title: Lung single cell 1439

description: lung single cell 1439


title: Lung single cell 1440

description: lung single cell 1440


title: Lung single cell 1434

description: lung single cell 1434


title: Lung single cell 1442

description: lung single cell 1442


title: Lung single cell 1436

description: lung single cell 1436


title: Lung single cell 1435

description: lung single cell 1435


title: Lung single cell 1428

description: lung single cell 1428


title: Lung single cell 1432

description: lung single cell 1432


title: Lung single cell 1429

description: lung single cell 1429


title: Lung single cell 1431

description: lung single cell 1431


title: Lung single cell 1427

description: lung single cell 1427


title: Lung single cell 1425

description: lung single cell 1425


title: Lung single cell 1421

description: lung single cell 1421


title: Lung single cell 1426

description: lung single cell 1426


title: Lung single cell 1423

description: lung single cell 1423


title: Lung single cell 1420

description: lung single cell 1420


title: Lung single cell 1419

description: lung single cell 1419


title: Lung single cell 1444

description: lung single cell 1444


title: Lung single cell 1418

description: lung single cell 1418


title: Lung single cell 1441

description: lung single cell 1441


title: Lung single cell 1415

description: lung single cell 1415


title: Lung single cell 1414

description: lung single cell 1414


title: Lung single cell 1417

description: lung single cell 1417


title: Lung single cell 1413

description: lung single cell 1413


title: Lung single cell 1411

description: lung single cell 1411


title: Lung single cell 1433

description: lung single cell 1433


title: Lung single cell 1409

description: lung single cell 1409


title: Lung single cell 1410

description: lung single cell 1410


title: Lung single cell 1407

description: lung single cell 1407


title: Lung single cell 1406

description: lung single cell 1406


title: Lung single cell 1405

description: lung single cell 1405


title: Lung single cell 1403

description: lung single cell 1403


title: Lung single cell 1404

description: lung single cell 1404


title: Lung single cell 1424

description: lung single cell 1424


title: Lung single cell 1402

description: lung single cell 1402


title: Lung single cell 1399

description: lung single cell 1399


title: Lung single cell 1400

description: lung single cell 1400


title: Lung single cell 1401

description: lung single cell 1401


title: Lung single cell 1398

description: lung single cell 1398


title: Lung single cell 1397

description: lung single cell 1397


title: Lung single cell 1395

description: lung single cell 1395


title: Lung single cell 1412

description: lung single cell 1412


title: Lung single cell 1396

description: lung single cell 1396


title: Lung single cell 1394

description: lung single cell 1394


title: Lung single cell 1393

description: lung single cell 1393


title: Lung single cell 1392

description: lung single cell 1392


title: Lung single cell 1391

description: lung single cell 1391


title: Lung single cell 1390

description: lung single cell 1390


title: Lung single cell 1388

description: lung single cell 1388


title: Lung single cell 1387

description: lung single cell 1387


title: Lung single cell 1389

description: lung single cell 1389


title: Lung single cell 1385

description: lung single cell 1385


title: Lung single cell 1384

description: lung single cell 1384


title: Lung single cell 1386

description: lung single cell 1386


title: Lung single cell 1382

description: lung single cell 1382


title: Lung single cell 1383

description: lung single cell 1383


title: Lung single cell 1381

description: lung single cell 1381


title: Lung single cell 1377

description: lung single cell 1377


title: Lung single cell 1380

description: lung single cell 1380


title: Lung single cell 1379

description: lung single cell 1379


title: Lung single cell 1378

description: lung single cell 1378


title: Lung single cell 1375

description: lung single cell 1375


title: Lung single cell 1373

description: lung single cell 1373


title: Lung single cell 1374

description: lung single cell 1374


title: Lung single cell 1372

description: lung single cell 1372


title: Lung single cell 1369

description: lung single cell 1369


title: Lung single cell 1367

description: lung single cell 1367


title: Lung single cell 1366

description: lung single cell 1366


title: Lung single cell 1371

description: lung single cell 1371


title: Lung single cell 1365

description: lung single cell 1365


title: Lung single cell 1368

description: lung single cell 1368


title: Lung single cell 1370

description: lung single cell 1370


title: Lung single cell 1364

description: lung single cell 1364


title: Lung single cell 1363

description: lung single cell 1363


title: Lung single cell 1360

description: lung single cell 1360


title: Lung single cell 1361

description: lung single cell 1361


title: Lung single cell 1362

description: lung single cell 1362


title: Lung single cell 1359

description: lung single cell 1359


title: Lung single cell 1357

description: lung single cell 1357


title: Lung single cell 1376

description: lung single cell 1376


title: Lung single cell 1408

description: lung single cell 1408


title: Lung single cell 1416

description: lung single cell 1416


title: Lung single cell 1355

description: lung single cell 1355


title: Lung single cell 1422

description: lung single cell 1422


title: Lung single cell 1353

description: lung single cell 1353


title: Lung single cell 1354

description: lung single cell 1354


title: Lung single cell 1358

description: lung single cell 1358


title: Lung single cell 1430

description: lung single cell 1430


title: Lung single cell 1356

description: lung single cell 1356


title: Lung single cell 1352

description: lung single cell 1352


title: Lung single cell 1351

description: lung single cell 1351


title: Lung single cell 1349

description: lung single cell 1349


title: Lung single cell 1348

description: lung single cell 1348


title: Lung single cell 1346

description: lung single cell 1346


title: Lung single cell 1347

description: lung single cell 1347


title: Lung single cell 1343

description: lung single cell 1343


title: Lung single cell 1339

description: lung single cell 1339


title: Lung single cell 1338

description: lung single cell 1338


title: Lung single cell 1344

description: lung single cell 1344


title: Lung single cell 1345

description: lung single cell 1345


title: Lung single cell 1342

description: lung single cell 1342


title: Lung single cell 1337

description: lung single cell 1337


title: Lung single cell 1340

description: lung single cell 1340


title: Lung single cell 1335

description: lung single cell 1335


title: Lung single cell 1331

description: lung single cell 1331


title: Lung single cell 1332

description: lung single cell 1332


title: Lung single cell 1350

description: lung single cell 1350


title: Lung single cell 1330

description: lung single cell 1330


title: Lung single cell 1334

description: lung single cell 1334


title: Lung single cell 1329

description: lung single cell 1329


title: Lung single cell 1326

description: lung single cell 1326


title: Lung single cell 1324

description: lung single cell 1324


title: Lung single cell 1327

description: lung single cell 1327


title: Lung single cell 1333

description: lung single cell 1333


title: Lung single cell 1328

description: lung single cell 1328


title: Lung single cell 1325

description: lung single cell 1325


title: Lung single cell 1322

description: lung single cell 1322


title: Lung single cell 1321

description: lung single cell 1321


title: Lung single cell 1320

description: lung single cell 1320


title: Lung single cell 1341

description: lung single cell 1341


title: Lung single cell 1318

description: lung single cell 1318


title: Lung single cell 1317

description: lung single cell 1317


title: Lung single cell 1315

description: lung single cell 1315


title: Lung single cell 1316

description: lung single cell 1316


title: Lung single cell 1336

description: lung single cell 1336


title: Lung single cell 1312

description: lung single cell 1312


title: Lung single cell 1313

description: lung single cell 1313


title: Lung single cell 1314

description: lung single cell 1314


title: Lung single cell 1311

description: lung single cell 1311


title: Lung single cell 1309

description: lung single cell 1309


title: Lung single cell 1305

description: lung single cell 1305


title: Lung single cell 1306

description: lung single cell 1306


title: Lung single cell 1299

description: lung single cell 1299


title: Lung single cell 1301

description: lung single cell 1301


title: Lung single cell 1302

description: lung single cell 1302


title: Lung single cell 1298

description: lung single cell 1298


title: Lung single cell 1304

description: lung single cell 1304


title: Lung single cell 1307

description: lung single cell 1307


title: Lung single cell 1300

description: lung single cell 1300


title: Lung single cell 1303

description: lung single cell 1303


title: Lung single cell 1323

description: lung single cell 1323


title: Lung single cell 1319

description: lung single cell 1319


title: Lung single cell 1294

description: lung single cell 1294


title: Lung single cell 1296

description: lung single cell 1296


title: Lung single cell 1310

description: lung single cell 1310


title: Lung single cell 1295

description: lung single cell 1295


title: Lung single cell 1292

description: lung single cell 1292


title: Lung single cell 1297

description: lung single cell 1297


title: Lung single cell 1293

description: lung single cell 1293


title: Lung single cell 1290

description: lung single cell 1290


title: Lung single cell 1288

description: lung single cell 1288


title: Lung single cell 1289

description: lung single cell 1289


title: Lung single cell 1287

description: lung single cell 1287


title: Lung single cell 1283

description: lung single cell 1283


title: Lung single cell 1285

description: lung single cell 1285


title: Lung single cell 1286

description: lung single cell 1286


title: Lung single cell 1284

description: lung single cell 1284


title: Lung single cell 1281

description: lung single cell 1281


title: Lung single cell 1280

description: lung single cell 1280


title: Lung single cell 1277

description: lung single cell 1277


title: Lung single cell 1279

description: lung single cell 1279


title: Lung single cell 1275

description: lung single cell 1275


title: Lung single cell 1276

description: lung single cell 1276


title: Lung single cell 1274

description: lung single cell 1274


title: Lung single cell 1437

description: lung single cell 1437


title: Lung single cell 1272

description: lung single cell 1272


title: Lung single cell 1271

description: lung single cell 1271


title: Lung single cell 1270

description: lung single cell 1270


title: Lung single cell 1269

description: lung single cell 1269


title: Lung single cell 1268

description: lung single cell 1268


title: Lung single cell 1291

description: lung single cell 1291


title: Lung single cell 1267

description: lung single cell 1267


title: Lung single cell 1263

description: lung single cell 1263


title: Lung single cell 1264

description: lung single cell 1264


title: Lung single cell 1265

description: lung single cell 1265


title: Lung single cell 1262

description: lung single cell 1262


title: Lung single cell 1261

description: lung single cell 1261


title: Lung single cell 1260

description: lung single cell 1260


title: Lung single cell 1258

description: lung single cell 1258


title: Lung single cell 1259

description: lung single cell 1259


title: Lung single cell 1282

description: lung single cell 1282


title: Lung single cell 1257

description: lung single cell 1257


title: Lung single cell 1278

description: lung single cell 1278


title: Lung single cell 1255

description: lung single cell 1255


title: Lung single cell 1273

description: lung single cell 1273


title: Lung single cell 1253

description: lung single cell 1253


title: Lung single cell 1252

description: lung single cell 1252


title: Lung single cell 1254

description: lung single cell 1254


title: Lung single cell 1266

description: lung single cell 1266


title: Lung single cell 1245

description: lung single cell 1245


title: Lung single cell 1247

description: lung single cell 1247


title: Lung single cell 1249

description: lung single cell 1249


title: Lung single cell 1246

description: lung single cell 1246


title: Lung single cell 1248

description: lung single cell 1248


title: Lung single cell 1243

description: lung single cell 1243


title: Lung single cell 1242

description: lung single cell 1242


title: Lung single cell 1239

description: lung single cell 1239


title: Lung single cell 1238

description: lung single cell 1238


title: Lung single cell 1240

description: lung single cell 1240


title: Lung single cell 1237

description: lung single cell 1237


title: Lung single cell 1256

description: lung single cell 1256


title: Lung single cell 1241

description: lung single cell 1241


title: Lung single cell 1235

description: lung single cell 1235


title: Lung single cell 1234

description: lung single cell 1234


title: Lung single cell 1236

description: lung single cell 1236


title: Lung single cell 1230

description: lung single cell 1230