title: 20150223_C94 E16.5 NR5A1-eGFP single-cell

status: Public on Feb 06 2018

city: Ramat Gan

first: Isabelle

last: Stévant

address: Institute of Nanotechnology and Advanced Materials, Building 206

country: Israel

institute: University of Bar Ilan

description:
20150223_C94
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt

data_processing:
Illumina Casava1.8.2 software used for basecalling.
FastQ files were mapped with GemTools (version 1.7.1) to the mouse reference genome (GRCm38.p3) and the genome annotation (version M4) downloaded from GENCODE, with an extra fake "Z" chromosome with the GFP sequence.
RPKM (Reads Per Kilobase of exon per Million reads mapped) were computed with an in-house pipeline.
Genome_build: mm10 (GRCm38)
Supplementary_files_format_and_content: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt: Tab-delimited text file includes RPKM values for each Sample.

source_name: XY gonad

extract_protocol:
XY gonads were collected in PBS at room temperature. PBS was removed and tissues were digested 10 minutes at 37°C with trypsin/EDTA 0.05% and filtered through a 40µm cell strainer to obtain single cell suspension. Nr5a1-eGFP+ cells were then sorted by fluorescent-active cell sorting (BD FACSARIA II) with Draq7TM dye to exclude dead cells and collected in PBS for the single-cell capture. Cells were centrifuged at low speed to be concentrated at an average of 400 million cells per mL prior cell capture. Single cells were captured on small size IFC microfluidic chips using the C1 Single-Cell Auto Prep System (Fluidigm) following the manufacturer recommended protocol. Reverse-transcription and pre-amplification of the single-cell cDNAs were achieved within the IFC chip using the SMARTer Ultra Low RNA kit for Illumina (Clontech) according to the C1 protocol.
RNA-sequencing libraries of the single-cell cDNA were prepared using the Illumina Nextera XT DNA Sample Preparation kit using the modified protocol described in the C1 documentation.

tag: strain/background

value: Mixed background

tag: cell type

value: NR5A1-eGFP positive cells

tag: c1 ifc size

value: small

tag: sequencing depth

value: 10M reads 100bp p-e


title: 20150223_C60 E16.5 NR5A1-eGFP single-cell

type: SRA

description:
20150223_C60
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt

library_source: transcriptomic

molecule: total RNA

organism: Mus musculus

tag: genotype/variation

tag: age


title: 20150223_C79 E16.5 NR5A1-eGFP single-cell

description:
20150223_C79
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C69 E16.5 NR5A1-eGFP single-cell

description:
20150223_C69
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C46 E16.5 NR5A1-eGFP single-cell

description:
20150223_C46
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C71 E16.5 NR5A1-eGFP single-cell

description:
20150223_C71
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C75 E16.5 NR5A1-eGFP single-cell

description:
20150223_C75
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C74 E16.5 NR5A1-eGFP single-cell

description:
20150223_C74
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C43 E16.5 NR5A1-eGFP single-cell

description:
20150223_C43
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C41 E16.5 NR5A1-eGFP single-cell

description:
20150223_C41
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C48 E16.5 NR5A1-eGFP single-cell

description:
20150223_C48
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C53 E16.5 NR5A1-eGFP single-cell

description:
20150223_C53
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C88 E16.5 NR5A1-eGFP single-cell

description:
20150223_C88
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C84 E16.5 NR5A1-eGFP single-cell

description:
20150223_C84
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C45 E16.5 NR5A1-eGFP single-cell

description:
20150223_C45
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C38 E16.5 NR5A1-eGFP single-cell

description:
20150223_C38
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C37 E16.5 NR5A1-eGFP single-cell

description:
20150223_C37
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C39 E16.5 NR5A1-eGFP single-cell

description:
20150223_C39
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C33 E16.5 NR5A1-eGFP single-cell

description:
20150223_C33
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C32 E16.5 NR5A1-eGFP single-cell

description:
20150223_C32
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C36 E16.5 NR5A1-eGFP single-cell

description:
20150223_C36
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C31 E16.5 NR5A1-eGFP single-cell

description:
20150223_C31
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C54 E16.5 NR5A1-eGFP single-cell

description:
20150223_C54
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C29 E16.5 NR5A1-eGFP single-cell

description:
20150223_C29
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C28 E16.5 NR5A1-eGFP single-cell

description:
20150223_C28
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C26 E16.5 NR5A1-eGFP single-cell

description:
20150223_C26
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C22 E16.5 NR5A1-eGFP single-cell

description:
20150223_C22
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C16 E16.5 NR5A1-eGFP single-cell

description:
20150223_C16
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C21 E16.5 NR5A1-eGFP single-cell

description:
20150223_C21
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C15 E16.5 NR5A1-eGFP single-cell

description:
20150223_C15
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C07 E16.5 NR5A1-eGFP single-cell

description:
20150223_C07
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C10 E16.5 NR5A1-eGFP single-cell

description:
20150223_C10
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C96 E16.5 NR5A1-eGFP single-cell

description:
20150202_C96
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C08 E16.5 NR5A1-eGFP single-cell

description:
20150223_C08
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C04 E16.5 NR5A1-eGFP single-cell

description:
20150223_C04
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C93 E16.5 NR5A1-eGFP single-cell

description:
20150202_C93
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C87 E16.5 NR5A1-eGFP single-cell

description:
20150202_C87
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C89 E16.5 NR5A1-eGFP single-cell

description:
20150202_C89
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C85 E16.5 NR5A1-eGFP single-cell

description:
20150202_C85
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C06 E16.5 NR5A1-eGFP single-cell

description:
20150223_C06
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C92 E16.5 NR5A1-eGFP single-cell

description:
20150202_C92
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C91 E16.5 NR5A1-eGFP single-cell

description:
20150202_C91
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C94 E16.5 NR5A1-eGFP single-cell

description:
20150202_C94
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150223_C30 E16.5 NR5A1-eGFP single-cell

description:
20150223_C30
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C81 E16.5 NR5A1-eGFP single-cell

description:
20150202_C81
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C82 E16.5 NR5A1-eGFP single-cell

description:
20150202_C82
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C83 E16.5 NR5A1-eGFP single-cell

description:
20150202_C83
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C76 E16.5 NR5A1-eGFP single-cell

description:
20150202_C76
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C77 E16.5 NR5A1-eGFP single-cell

description:
20150202_C77
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C78 E16.5 NR5A1-eGFP single-cell

description:
20150202_C78
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C75 E16.5 NR5A1-eGFP single-cell

description:
20150202_C75
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C72 E16.5 NR5A1-eGFP single-cell

description:
20150202_C72
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C74 E16.5 NR5A1-eGFP single-cell

description:
20150202_C74
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C79 E16.5 NR5A1-eGFP single-cell

description:
20150202_C79
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C95 E16.5 NR5A1-eGFP single-cell

description:
20150202_C95
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C68 E16.5 NR5A1-eGFP single-cell

description:
20150202_C68
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C69 E16.5 NR5A1-eGFP single-cell

description:
20150202_C69
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C90 E16.5 NR5A1-eGFP single-cell

description:
20150202_C90
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C70 E16.5 NR5A1-eGFP single-cell

description:
20150202_C70
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C66 E16.5 NR5A1-eGFP single-cell

description:
20150202_C66
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C64 E16.5 NR5A1-eGFP single-cell

description:
20150202_C64
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C67 E16.5 NR5A1-eGFP single-cell

description:
20150202_C67
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C63 E16.5 NR5A1-eGFP single-cell

description:
20150202_C63
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C52 E16.5 NR5A1-eGFP single-cell

description:
20150202_C52
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C55 E16.5 NR5A1-eGFP single-cell

description:
20150202_C55
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C57 E16.5 NR5A1-eGFP single-cell

description:
20150202_C57
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C61 E16.5 NR5A1-eGFP single-cell

description:
20150202_C61
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C51 E16.5 NR5A1-eGFP single-cell

description:
20150202_C51
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C58 E16.5 NR5A1-eGFP single-cell

description:
20150202_C58
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C60 E16.5 NR5A1-eGFP single-cell

description:
20150202_C60
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C56 E16.5 NR5A1-eGFP single-cell

description:
20150202_C56
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C50 E16.5 NR5A1-eGFP single-cell

description:
20150202_C50
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C48 E16.5 NR5A1-eGFP single-cell

description:
20150202_C48
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C49 E16.5 NR5A1-eGFP single-cell

description:
20150202_C49
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C47 E16.5 NR5A1-eGFP single-cell

description:
20150202_C47
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C46 E16.5 NR5A1-eGFP single-cell

description:
20150202_C46
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150202_C27 E16.5 NR5A1-eGFP single-cell

description:
20150202_C27
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C96 E13.5 NR5A1-eGFP single-cell

description:
20140528_C96
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt

value: 25M reads 100bp p-e


title: 20140528_C91 E13.5 NR5A1-eGFP single-cell

description:
20140528_C91
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C92 E13.5 NR5A1-eGFP single-cell

description:
20140528_C92
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C89 E13.5 NR5A1-eGFP single-cell

description:
20140528_C89
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C88 E13.5 NR5A1-eGFP single-cell

description:
20140528_C88
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C90 E13.5 NR5A1-eGFP single-cell

description:
20140528_C90
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C86 E13.5 NR5A1-eGFP single-cell

description:
20140528_C86
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C85 E13.5 NR5A1-eGFP single-cell

description:
20140528_C85
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C83 E13.5 NR5A1-eGFP single-cell

description:
20140528_C83
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C76 E13.5 NR5A1-eGFP single-cell

description:
20140528_C76
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C81 E13.5 NR5A1-eGFP single-cell

description:
20140528_C81
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C78 E13.5 NR5A1-eGFP single-cell

description:
20140528_C78
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C82 E13.5 NR5A1-eGFP single-cell

description:
20140528_C82
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C73 E13.5 NR5A1-eGFP single-cell

description:
20140528_C73
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C75 E13.5 NR5A1-eGFP single-cell

description:
20140528_C75
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C68 E13.5 NR5A1-eGFP single-cell

description:
20140528_C68
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C65 E13.5 NR5A1-eGFP single-cell

description:
20140528_C65
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C62 E13.5 NR5A1-eGFP single-cell

description:
20140528_C62
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C67 E13.5 NR5A1-eGFP single-cell

description:
20140528_C67
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C93 E13.5 NR5A1-eGFP single-cell

description:
20140528_C93
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C66 E13.5 NR5A1-eGFP single-cell

description:
20140528_C66
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C60 E13.5 NR5A1-eGFP single-cell

description:
20140528_C60
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C59 E13.5 NR5A1-eGFP single-cell

description:
20140528_C59
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C58 E13.5 NR5A1-eGFP single-cell

description:
20140528_C58
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C53 E13.5 NR5A1-eGFP single-cell

description:
20140528_C53
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C84 E13.5 NR5A1-eGFP single-cell

description:
20140528_C84
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C56 E13.5 NR5A1-eGFP single-cell

description:
20140528_C56
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C55 E13.5 NR5A1-eGFP single-cell

description:
20140528_C55
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C51 E13.5 NR5A1-eGFP single-cell

description:
20140528_C51
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C49 E13.5 NR5A1-eGFP single-cell

description:
20140528_C49
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C45 E13.5 NR5A1-eGFP single-cell

description:
20140528_C45
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C42 E13.5 NR5A1-eGFP single-cell

description:
20140528_C42
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C41 E13.5 NR5A1-eGFP single-cell

description:
20140528_C41
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C40 E13.5 NR5A1-eGFP single-cell

description:
20140528_C40
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C70 E13.5 NR5A1-eGFP single-cell

description:
20140528_C70
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C44 E13.5 NR5A1-eGFP single-cell

description:
20140528_C44
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C63 E13.5 NR5A1-eGFP single-cell

description:
20140528_C63
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C61 E13.5 NR5A1-eGFP single-cell

description:
20140528_C61
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C36 E13.5 NR5A1-eGFP single-cell

description:
20140528_C36
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C32 E13.5 NR5A1-eGFP single-cell

description:
20140528_C32
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C38 E13.5 NR5A1-eGFP single-cell

description:
20140528_C38
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C33 E13.5 NR5A1-eGFP single-cell

description:
20140528_C33
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C35 E13.5 NR5A1-eGFP single-cell

description:
20140528_C35
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C27 E13.5 NR5A1-eGFP single-cell

description:
20140528_C27
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C29 E13.5 NR5A1-eGFP single-cell

description:
20140528_C29
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C28 E13.5 NR5A1-eGFP single-cell

description:
20140528_C28
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C23 E13.5 NR5A1-eGFP single-cell

description:
20140528_C23
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C22 E13.5 NR5A1-eGFP single-cell

description:
20140528_C22
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C54 E13.5 NR5A1-eGFP single-cell

description:
20140528_C54
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C57 E13.5 NR5A1-eGFP single-cell

description:
20140528_C57
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C48 E13.5 NR5A1-eGFP single-cell

description:
20140528_C48
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C21 E13.5 NR5A1-eGFP single-cell

description:
20140528_C21
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C20 E13.5 NR5A1-eGFP single-cell

description:
20140528_C20
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C18 E13.5 NR5A1-eGFP single-cell

description:
20140528_C18
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C19 E13.5 NR5A1-eGFP single-cell

description:
20140528_C19
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C39 E13.5 NR5A1-eGFP single-cell

description:
20140528_C39
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C13 E13.5 NR5A1-eGFP single-cell

description:
20140528_C13
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C11 E13.5 NR5A1-eGFP single-cell

description:
20140528_C11
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C12 E13.5 NR5A1-eGFP single-cell

description:
20140528_C12
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C91 E13.5 NR5A1-eGFP single-cell

description:
20130918_C91
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt

value: medium


title: 20140528_C07 E13.5 NR5A1-eGFP single-cell

description:
20140528_C07
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C89 E13.5 NR5A1-eGFP single-cell

description:
20130918_C89
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C87 E13.5 NR5A1-eGFP single-cell

description:
20130918_C87
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C85 E13.5 NR5A1-eGFP single-cell

description:
20130918_C85
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C82 E13.5 NR5A1-eGFP single-cell

description:
20130918_C82
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C31 E13.5 NR5A1-eGFP single-cell

description:
20140528_C31
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C81 E13.5 NR5A1-eGFP single-cell

description:
20130918_C81
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C77 E13.5 NR5A1-eGFP single-cell

description:
20130918_C77
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C75 E13.5 NR5A1-eGFP single-cell

description:
20130918_C75
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C67 E13.5 NR5A1-eGFP single-cell

description:
20130918_C67
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C61 E13.5 NR5A1-eGFP single-cell

description:
20130918_C61
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C60 E13.5 NR5A1-eGFP single-cell

description:
20130918_C60
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C66 E13.5 NR5A1-eGFP single-cell

description:
20130918_C66
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C63 E13.5 NR5A1-eGFP single-cell

description:
20130918_C63
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C58 E13.5 NR5A1-eGFP single-cell

description:
20130918_C58
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C53 E13.5 NR5A1-eGFP single-cell

description:
20130918_C53
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C48 E13.5 NR5A1-eGFP single-cell

description:
20130918_C48
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C55 E13.5 NR5A1-eGFP single-cell

description:
20130918_C55
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C15 E13.5 NR5A1-eGFP single-cell

description:
20140528_C15
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C49 E13.5 NR5A1-eGFP single-cell

description:
20130918_C49
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C50 E13.5 NR5A1-eGFP single-cell

description:
20130918_C50
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C47 E13.5 NR5A1-eGFP single-cell

description:
20130918_C47
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C46 E13.5 NR5A1-eGFP single-cell

description:
20130918_C46
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C06 E13.5 NR5A1-eGFP single-cell

description:
20140528_C06
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C45 E13.5 NR5A1-eGFP single-cell

description:
20130918_C45
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C42 E13.5 NR5A1-eGFP single-cell

description:
20130918_C42
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C43 E13.5 NR5A1-eGFP single-cell

description:
20130918_C43
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C40 E13.5 NR5A1-eGFP single-cell

description:
20130918_C40
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C41 E13.5 NR5A1-eGFP single-cell

description:
20130918_C41
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C44 E13.5 NR5A1-eGFP single-cell

description:
20130918_C44
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C36 E13.5 NR5A1-eGFP single-cell

description:
20130918_C36
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C38 E13.5 NR5A1-eGFP single-cell

description:
20130918_C38
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C35 E13.5 NR5A1-eGFP single-cell

description:
20130918_C35
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C37 E13.5 NR5A1-eGFP single-cell

description:
20130918_C37
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C34 E13.5 NR5A1-eGFP single-cell

description:
20130918_C34
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C33 E13.5 NR5A1-eGFP single-cell

description:
20130918_C33
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C21 E13.5 NR5A1-eGFP single-cell

description:
20130918_C21
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C28 E13.5 NR5A1-eGFP single-cell

description:
20130918_C28
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C29 E13.5 NR5A1-eGFP single-cell

description:
20130918_C29
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C24 E13.5 NR5A1-eGFP single-cell

description:
20130918_C24
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C31 E13.5 NR5A1-eGFP single-cell

description:
20130918_C31
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C18 E13.5 NR5A1-eGFP single-cell

description:
20130918_C18
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C22 E13.5 NR5A1-eGFP single-cell

description:
20130918_C22
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C20 E13.5 NR5A1-eGFP single-cell

description:
20130918_C20
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C26 E13.5 NR5A1-eGFP single-cell

description:
20130918_C26
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C25 E13.5 NR5A1-eGFP single-cell

description:
20130918_C25
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C15 E13.5 NR5A1-eGFP single-cell

description:
20130918_C15
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C17 E13.5 NR5A1-eGFP single-cell

description:
20130918_C17
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C12 E13.5 NR5A1-eGFP single-cell

description:
20130918_C12
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C08 E13.5 NR5A1-eGFP single-cell

description:
20130918_C08
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C10 E13.5 NR5A1-eGFP single-cell

description:
20130918_C10
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C11 E13.5 NR5A1-eGFP single-cell

description:
20130918_C11
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C06 E13.5 NR5A1-eGFP single-cell

description:
20130918_C06
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C03 E13.5 NR5A1-eGFP single-cell

description:
20130918_C03
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C32 E13.5 NR5A1-eGFP single-cell

description:
20130918_C32
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C04 E13.5 NR5A1-eGFP single-cell

description:
20130918_C04
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C96 E12.5 NR5A1-eGFP single-cell

description:
20141210_C96
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C92 E12.5 NR5A1-eGFP single-cell

description:
20141210_C92
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C02 E13.5 NR5A1-eGFP single-cell

description:
20130918_C02
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C91 E12.5 NR5A1-eGFP single-cell

description:
20141210_C91
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C89 E12.5 NR5A1-eGFP single-cell

description:
20141210_C89
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C90 E12.5 NR5A1-eGFP single-cell

description:
20141210_C90
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C23 E13.5 NR5A1-eGFP single-cell

description:
20130918_C23
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C85 E12.5 NR5A1-eGFP single-cell

description:
20141210_C85
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C65 E12.5 NR5A1-eGFP single-cell

description:
20141210_C65
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C72 E12.5 NR5A1-eGFP single-cell

description:
20141210_C72
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C84 E12.5 NR5A1-eGFP single-cell

description:
20141210_C84
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140528_C16 E13.5 NR5A1-eGFP single-cell

description:
20140528_C16
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C76 E12.5 NR5A1-eGFP single-cell

description:
20141210_C76
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C79 E12.5 NR5A1-eGFP single-cell

description:
20141210_C79
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C82 E12.5 NR5A1-eGFP single-cell

description:
20141210_C82
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C86 E12.5 NR5A1-eGFP single-cell

description:
20141210_C86
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C64 E12.5 NR5A1-eGFP single-cell

description:
20141210_C64
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C63 E12.5 NR5A1-eGFP single-cell

description:
20141210_C63
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C56 E12.5 NR5A1-eGFP single-cell

description:
20141210_C56
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C50 E12.5 NR5A1-eGFP single-cell

description:
20141210_C50
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C61 E12.5 NR5A1-eGFP single-cell

description:
20141210_C61
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C51 E12.5 NR5A1-eGFP single-cell

description:
20141210_C51
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C59 E12.5 NR5A1-eGFP single-cell

description:
20141210_C59
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C81 E12.5 NR5A1-eGFP single-cell

description:
20141210_C81
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20130918_C16 E13.5 NR5A1-eGFP single-cell

description:
20130918_C16
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C43 E12.5 NR5A1-eGFP single-cell

description:
20141210_C43
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C44 E12.5 NR5A1-eGFP single-cell

description:
20141210_C44
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C42 E12.5 NR5A1-eGFP single-cell

description:
20141210_C42
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C87 E12.5 NR5A1-eGFP single-cell

description:
20141210_C87
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C41 E12.5 NR5A1-eGFP single-cell

description:
20141210_C41
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C45 E12.5 NR5A1-eGFP single-cell

description:
20141210_C45
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C47 E12.5 NR5A1-eGFP single-cell

description:
20141210_C47
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C83 E12.5 NR5A1-eGFP single-cell

description:
20141210_C83
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C69 E12.5 NR5A1-eGFP single-cell

description:
20141210_C69
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C70 E12.5 NR5A1-eGFP single-cell

description:
20141210_C70
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C49 E12.5 NR5A1-eGFP single-cell

description:
20141210_C49
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C46 E12.5 NR5A1-eGFP single-cell

description:
20141210_C46
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C40 E12.5 NR5A1-eGFP single-cell

description:
20141210_C40
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C35 E12.5 NR5A1-eGFP single-cell

description:
20141210_C35
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C30 E12.5 NR5A1-eGFP single-cell

description:
20141210_C30
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C37 E12.5 NR5A1-eGFP single-cell

description:
20141210_C37
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140526_C95 E12.5 NR5A1-eGFP single-cell

description:
20140526_C95
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C39 E12.5 NR5A1-eGFP single-cell

description:
20141210_C39
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C31 E12.5 NR5A1-eGFP single-cell

description:
20141210_C31
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C13 E12.5 NR5A1-eGFP single-cell

description:
20141210_C13
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C10 E12.5 NR5A1-eGFP single-cell

description:
20141210_C10
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140526_C85 E12.5 NR5A1-eGFP single-cell

description:
20140526_C85
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140526_C80 E12.5 NR5A1-eGFP single-cell

description:
20140526_C80
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140526_C93 E12.5 NR5A1-eGFP single-cell

description:
20140526_C93
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C17 E12.5 NR5A1-eGFP single-cell

description:
20141210_C17
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140526_C90 E12.5 NR5A1-eGFP single-cell

description:
20140526_C90
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C09 E12.5 NR5A1-eGFP single-cell

description:
20141210_C09
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140526_C89 E12.5 NR5A1-eGFP single-cell

description:
20140526_C89
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140526_C76 E12.5 NR5A1-eGFP single-cell

description:
20140526_C76
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140526_C74 E12.5 NR5A1-eGFP single-cell

description:
20140526_C74
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140526_C75 E12.5 NR5A1-eGFP single-cell

description:
20140526_C75
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140526_C71 E12.5 NR5A1-eGFP single-cell

description:
20140526_C71
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C16 E12.5 NR5A1-eGFP single-cell

description:
20141210_C16
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140526_C72 E12.5 NR5A1-eGFP single-cell

description:
20140526_C72
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140526_C70 E12.5 NR5A1-eGFP single-cell

description:
20140526_C70
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140526_C69 E12.5 NR5A1-eGFP single-cell

description:
20140526_C69
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140526_C68 E12.5 NR5A1-eGFP single-cell

description:
20140526_C68
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C36 E12.5 NR5A1-eGFP single-cell

description:
20141210_C36
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141210_C26 E12.5 NR5A1-eGFP single-cell

description:
20141210_C26
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140526_C48 E12.5 NR5A1-eGFP single-cell

description:
20140526_C48
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140526_C62 E12.5 NR5A1-eGFP single-cell

description:
20140526_C62
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140526_C47 E12.5 NR5A1-eGFP single-cell

description:
20140526_C47
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140526_C58 E12.5 NR5A1-eGFP single-cell

description:
20140526_C58
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140526_C45 E12.5 NR5A1-eGFP single-cell

description:
20140526_C45
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140526_C43 E12.5 NR5A1-eGFP single-cell

description:
20140526_C43
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140526_C42 E12.5 NR5A1-eGFP single-cell

description:
20140526_C42
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140526_C38 E12.5 NR5A1-eGFP single-cell

description:
20140526_C38
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140526_C35 E12.5 NR5A1-eGFP single-cell

description:
20140526_C35
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140526_C32 E12.5 NR5A1-eGFP single-cell

description:
20140526_C32
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140526_C28 E12.5 NR5A1-eGFP single-cell

description:
20140526_C28
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140526_C08 E12.5 NR5A1-eGFP single-cell

description:
20140526_C08
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C96 E11.5 NR5A1-eGFP single-cell

description:
20150107_C96
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140526_C91 E12.5 NR5A1-eGFP single-cell

description:
20140526_C91
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C87 E11.5 NR5A1-eGFP single-cell

description:
20150107_C87
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C90 E11.5 NR5A1-eGFP single-cell

description:
20150107_C90
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C86 E11.5 NR5A1-eGFP single-cell

description:
20150107_C86
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C80 E11.5 NR5A1-eGFP single-cell

description:
20150107_C80
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C79 E11.5 NR5A1-eGFP single-cell

description:
20150107_C79
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C93 E11.5 NR5A1-eGFP single-cell

description:
20150107_C93
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C78 E11.5 NR5A1-eGFP single-cell

description:
20150107_C78
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C84 E11.5 NR5A1-eGFP single-cell

description:
20150107_C84
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C77 E11.5 NR5A1-eGFP single-cell

description:
20150107_C77
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C76 E11.5 NR5A1-eGFP single-cell

description:
20150107_C76
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: WT (T01)

city: Suzhou

first: Han

last: Wang

state: JIangsu

address: 199 Renai, SIP

department: Center for Circadian Clocks

country: China

institute: Soochow University

data_processing:
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to zebrafish GRCz10 whole genome using bowtie v0.12.2 with parameters -q -p 4 -e 100 -y -a -m 10 --best --strata
Cufflinks are used for assemble transcripts and remap and quanlity gene-expression level, which is estimate by FPKM value.
Supplementary_files_format_and_content: Tab-delimited text files include FPKM values for each Sample …

organism: Danio rerio

growth_protocol: Zebrafish  embryos were obtained by natural cross and fertilized eggs were raised at 28.5°C and maintained on a 14-hours light/10-hours dark cycle.

extract_protocol:
Zebrafish embyros were collected and RNAs were extracted using Trizol reagent. RNA integrity and concentration were checked using NanoDrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA) and an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).
mRNAs were isolated by NEBNext Poly (A) mRNA Magnetic Isolation Module (NEB, E7490). The cDNA library was constructed following the manufacturer’s instructions of NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, E7530) and NEBNext Multiplex Oligos for Illumina (NEB, E7500).

tag: strain background

value: AB

tag: tissue

value: whole embryos

tag: Stage

value: 28 hours post fertilization


title: GFP-neg surrounding cells 1 Technical 1

city: Omaha

first: David

last: He

state: Nebraska

address: 2500 California Plaza

department: Department of Biomedical Sciences

country: USA

institute: Creighton University

description:
This biological replicate has two technical replicates which are represented by the two raw files.
fish_WT_CGTACTAG-_L001_R1_001

data_processing:
CLC Genomics Workbench software (CLC bio, Waltham, MA, USA) was used to map the reads to the GRCz10 zebrafish genome and generate gene expression values in the normalized form of reads per kilobase of transcript per million mapped reads (RPKM) values
Genome_build: GRCz10 zebrafish genome
Supplementary_files_format_and_content: Expression data was exported from CLC Genomics Workbench in the form of Microsoft Excel Spreadsheets.

source_name: 2,000 Inner ear surrounding cells

extract_protocol:
Qiagen miRNeasy mini plus kit
Clontech Smart-Seq

value: GFP-neg cells isolated and pooled from  the lagena, utricle, and saccule

value: 11-13 month old adult

tag: Sex

value: female

tag: strain


title: 20140526_C40 E12.5 NR5A1-eGFP single-cell

description:
20140526_C40
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: GFP+ Hair Cells Biological Replicate 2 Technical 1

description:
This biological replicate has two technical replicates which are represented by the two raw files.
zhc_AGGCAGAA-_L001_R1_001

source_name: 2,000 Inner ear hair cells

value: GFP+ Hair Cells isolated and pooled from the lagena, utricle, and saccule


title: 20150107_C73 E11.5 NR5A1-eGFP single-cell

description:
20150107_C73
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C75 E11.5 NR5A1-eGFP single-cell

description:
20150107_C75
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C72 E11.5 NR5A1-eGFP single-cell

description:
20150107_C72
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140526_C22 E12.5 NR5A1-eGFP single-cell

description:
20140526_C22
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: GFP+ Hair Cells Biological Replicate 2 Technical 2

description:
This biological replicate has two technical replicates which are represented by the two raw files.
zhc_AGGCAGAA-_L002_R1_001


title: GFP+ Hair Cells Biological Replicate 1 Technical 1

description:
This biological replicate has two technical replicates which are represented by the two raw files.
fish_TAAGGCGA-_L001_R1_001


title: 20150107_C71 E11.5 NR5A1-eGFP single-cell

description:
20150107_C71
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: GFP+ Hair Cells Biological Replicate 1 Technical 2

description:
This biological replicate has two technical replicates which are represented by the two raw files.
fish_TAAGGCGA-_L002_R1_001


title: 20150107_C67 E11.5 NR5A1-eGFP single-cell

description:
20150107_C67
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C68 E11.5 NR5A1-eGFP single-cell

description:
20150107_C68
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C66 E11.5 NR5A1-eGFP single-cell

description:
20150107_C66
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C69 E11.5 NR5A1-eGFP single-cell

description:
20150107_C69
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: ezh2-/- (T02)


title: 20140526_C01 E12.5 NR5A1-eGFP single-cell

description:
20140526_C01
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C54 E11.5 NR5A1-eGFP single-cell

description:
20150107_C54
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C64 E11.5 NR5A1-eGFP single-cell

description:
20150107_C64
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C53 E11.5 NR5A1-eGFP single-cell

description:
20150107_C53
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C65 E11.5 NR5A1-eGFP single-cell

description:
20150107_C65
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C58 E11.5 NR5A1-eGFP single-cell

description:
20150107_C58
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C48 E11.5 NR5A1-eGFP single-cell

description:
20150107_C48
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C46 E11.5 NR5A1-eGFP single-cell

description:
20150107_C46
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C43 E11.5 NR5A1-eGFP single-cell

description:
20150107_C43
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C41 E11.5 NR5A1-eGFP single-cell

description:
20150107_C41
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C51 E11.5 NR5A1-eGFP single-cell

description:
20150107_C51
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C47 E11.5 NR5A1-eGFP single-cell

description:
20150107_C47
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C52 E11.5 NR5A1-eGFP single-cell

description:
20150107_C52
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: GFP-neg surrounding cells 1 Technical 2

description:
This biological replicate has two technical replicates which are represented by the two raw files.
fish_WT_CGTACTAG-_L002_R1_001


title: 20150107_C40 E11.5 NR5A1-eGFP single-cell

description:
20150107_C40
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C37 E11.5 NR5A1-eGFP single-cell

description:
20150107_C37
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C30 E11.5 NR5A1-eGFP single-cell

description:
20150107_C30
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C35 E11.5 NR5A1-eGFP single-cell

description:
20150107_C35
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C36 E11.5 NR5A1-eGFP single-cell

description:
20150107_C36
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C28 E11.5 NR5A1-eGFP single-cell

description:
20150107_C28
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C11 E11.5 NR5A1-eGFP single-cell

description:
20150107_C11
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C24 E11.5 NR5A1-eGFP single-cell

description:
20150107_C24
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C03 E11.5 NR5A1-eGFP single-cell

description:
20150107_C03
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C18 E11.5 NR5A1-eGFP single-cell

description:
20150107_C18
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C95 E11.5 NR5A1-eGFP single-cell

description:
20141103_C95
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20150107_C25 E11.5 NR5A1-eGFP single-cell

description:
20150107_C25
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C87 E11.5 NR5A1-eGFP single-cell

description:
20141103_C87
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C89 E11.5 NR5A1-eGFP single-cell

description:
20141103_C89
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C84 E11.5 NR5A1-eGFP single-cell

description:
20141103_C84
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C88 E11.5 NR5A1-eGFP single-cell

description:
20141103_C88
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C82 E11.5 NR5A1-eGFP single-cell

description:
20141103_C82
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C79 E11.5 NR5A1-eGFP single-cell

description:
20141103_C79
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C76 E11.5 NR5A1-eGFP single-cell

description:
20141103_C76
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C75 E11.5 NR5A1-eGFP single-cell

description:
20141103_C75
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C73 E11.5 NR5A1-eGFP single-cell

description:
20141103_C73
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C80 E11.5 NR5A1-eGFP single-cell

description:
20141103_C80
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C77 E11.5 NR5A1-eGFP single-cell

description:
20141103_C77
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C60 E11.5 NR5A1-eGFP single-cell

description:
20141103_C60
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C81 E11.5 NR5A1-eGFP single-cell

description:
20141103_C81
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C59 E11.5 NR5A1-eGFP single-cell

description:
20141103_C59
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C70 E11.5 NR5A1-eGFP single-cell

description:
20141103_C70
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C62 E11.5 NR5A1-eGFP single-cell

description:
20141103_C62
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C65 E11.5 NR5A1-eGFP single-cell

description:
20141103_C65
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C57 E11.5 NR5A1-eGFP single-cell

description:
20141103_C57
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C53 E11.5 NR5A1-eGFP single-cell

description:
20141103_C53
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C61 E11.5 NR5A1-eGFP single-cell

description:
20141103_C61
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C58 E11.5 NR5A1-eGFP single-cell

description:
20141103_C58
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C56 E11.5 NR5A1-eGFP single-cell

description:
20141103_C56
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C54 E11.5 NR5A1-eGFP single-cell

description:
20141103_C54
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C33 E11.5 NR5A1-eGFP single-cell

description:
20141103_C33
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C32 E11.5 NR5A1-eGFP single-cell

description:
20141103_C32
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C28 E11.5 NR5A1-eGFP single-cell

description:
20141103_C28
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C42 E11.5 NR5A1-eGFP single-cell

description:
20141103_C42
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C41 E11.5 NR5A1-eGFP single-cell

description:
20141103_C41
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C30 E11.5 NR5A1-eGFP single-cell

description:
20141103_C30
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C25 E11.5 NR5A1-eGFP single-cell

description:
20141103_C25
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C29 E11.5 NR5A1-eGFP single-cell

description:
20141103_C29
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C50 E11.5 NR5A1-eGFP single-cell

description:
20141103_C50
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C39 E11.5 NR5A1-eGFP single-cell

description:
20141103_C39
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C48 E11.5 NR5A1-eGFP single-cell

description:
20141103_C48
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C22 E11.5 NR5A1-eGFP single-cell

description:
20141103_C22
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C49 E11.5 NR5A1-eGFP single-cell

description:
20141103_C49
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C35 E11.5 NR5A1-eGFP single-cell

description:
20141103_C35
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C24 E11.5 NR5A1-eGFP single-cell

description:
20141103_C24
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C18 E11.5 NR5A1-eGFP single-cell

description:
20141103_C18
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C17 E11.5 NR5A1-eGFP single-cell

description:
20141103_C17
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C52 E11.5 NR5A1-eGFP single-cell

description:
20141103_C52
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C16 E11.5 NR5A1-eGFP single-cell

description:
20141103_C16
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C09 E11.5 NR5A1-eGFP single-cell

description:
20141103_C09
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C47 E11.5 NR5A1-eGFP single-cell

description:
20141103_C47
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C12 E11.5 NR5A1-eGFP single-cell

description:
20141103_C12
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C15 E11.5 NR5A1-eGFP single-cell

description:
20141103_C15
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C11 E11.5 NR5A1-eGFP single-cell

description:
20141103_C11
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C14 E11.5 NR5A1-eGFP single-cell

description:
20141103_C14
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C08 E11.5 NR5A1-eGFP single-cell

description:
20141103_C08
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C07 E11.5 NR5A1-eGFP single-cell

description:
20141103_C07
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C40 E11.5 NR5A1-eGFP single-cell

description:
20141103_C40
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C21 E11.5 NR5A1-eGFP single-cell

description:
20141103_C21
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C05 E11.5 NR5A1-eGFP single-cell

description:
20141103_C05
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C90 E10.5 NR5A1-eGFP single-cell

description:
20140428_C90
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C94 E10.5 NR5A1-eGFP single-cell

description:
20140428_C94
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C93 E10.5 NR5A1-eGFP single-cell

description:
20140428_C93
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C95 E10.5 NR5A1-eGFP single-cell

description:
20140428_C95
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C77 E10.5 NR5A1-eGFP single-cell

description:
20140428_C77
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C78 E10.5 NR5A1-eGFP single-cell

description:
20140428_C78
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C85 E10.5 NR5A1-eGFP single-cell

description:
20140428_C85
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C88 E10.5 NR5A1-eGFP single-cell

description:
20140428_C88
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C72 E10.5 NR5A1-eGFP single-cell

description:
20140428_C72
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C81 E10.5 NR5A1-eGFP single-cell

description:
20140428_C81
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C92 E10.5 NR5A1-eGFP single-cell

description:
20140428_C92
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C89 E10.5 NR5A1-eGFP single-cell

description:
20140428_C89
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C75 E10.5 NR5A1-eGFP single-cell

description:
20140428_C75
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C03 E11.5 NR5A1-eGFP single-cell

description:
20141103_C03
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C73 E10.5 NR5A1-eGFP single-cell

description:
20140428_C73
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C76 E10.5 NR5A1-eGFP single-cell

description:
20140428_C76
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C82 E10.5 NR5A1-eGFP single-cell

description:
20140428_C82
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C66 E10.5 NR5A1-eGFP single-cell

description:
20140428_C66
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C68 E10.5 NR5A1-eGFP single-cell

description:
20140428_C68
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C65 E10.5 NR5A1-eGFP single-cell

description:
20140428_C65
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C69 E10.5 NR5A1-eGFP single-cell

description:
20140428_C69
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C63 E10.5 NR5A1-eGFP single-cell

description:
20140428_C63
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C62 E10.5 NR5A1-eGFP single-cell

description:
20140428_C62
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C61 E10.5 NR5A1-eGFP single-cell

description:
20140428_C61
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C57 E10.5 NR5A1-eGFP single-cell

description:
20140428_C57
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C79 E10.5 NR5A1-eGFP single-cell

description:
20140428_C79
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C56 E10.5 NR5A1-eGFP single-cell

description:
20140428_C56
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C70 E10.5 NR5A1-eGFP single-cell

description:
20140428_C70
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C55 E10.5 NR5A1-eGFP single-cell

description:
20140428_C55
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C51 E10.5 NR5A1-eGFP single-cell

description:
20140428_C51
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C44 E10.5 NR5A1-eGFP single-cell

description:
20140428_C44
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C43 E10.5 NR5A1-eGFP single-cell

description:
20140428_C43
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C45 E10.5 NR5A1-eGFP single-cell

description:
20140428_C45
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C27 E10.5 NR5A1-eGFP single-cell

description:
20140428_C27
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C34 E10.5 NR5A1-eGFP single-cell

description:
20140428_C34
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C41 E10.5 NR5A1-eGFP single-cell

description:
20140428_C41
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C42 E10.5 NR5A1-eGFP single-cell

description:
20140428_C42
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C19 E10.5 NR5A1-eGFP single-cell

description:
20140428_C19
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C23 E10.5 NR5A1-eGFP single-cell

description:
20140428_C23
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C31 E10.5 NR5A1-eGFP single-cell

description:
20140428_C31
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C26 E10.5 NR5A1-eGFP single-cell

description:
20140428_C26
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C20 E10.5 NR5A1-eGFP single-cell

description:
20140428_C20
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C15 E10.5 NR5A1-eGFP single-cell

description:
20140428_C15
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C32 E10.5 NR5A1-eGFP single-cell

description:
20140428_C32
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C13 E10.5 NR5A1-eGFP single-cell

description:
20140428_C13
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C11 E10.5 NR5A1-eGFP single-cell

description:
20140428_C11
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20141103_C27 E11.5 NR5A1-eGFP single-cell

description:
20141103_C27
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C53 E10.5 NR5A1-eGFP single-cell

description:
20140428_C53
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C52 E10.5 NR5A1-eGFP single-cell

description:
20140428_C52
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C10 E10.5 NR5A1-eGFP single-cell

description:
20140428_C10
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C39 E10.5 NR5A1-eGFP single-cell

description:
20140428_C39
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C35 E10.5 NR5A1-eGFP single-cell

description:
20140428_C35
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C49 E10.5 NR5A1-eGFP single-cell

description:
20140428_C49
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C07 E10.5 NR5A1-eGFP single-cell

description:
20140428_C07
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C24 E10.5 NR5A1-eGFP single-cell

description:
20140428_C24
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C06 E10.5 NR5A1-eGFP single-cell

description:
20140428_C06
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C33 E10.5 NR5A1-eGFP single-cell

description:
20140428_C33
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C12 E10.5 NR5A1-eGFP single-cell

description:
20140428_C12
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C01 E10.5 NR5A1-eGFP single-cell

description:
20140428_C01
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C02 E10.5 NR5A1-eGFP single-cell

description:
20140428_C02
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: 20140428_C09 E10.5 NR5A1-eGFP single-cell

description:
20140428_C09
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: heart1_2dpf_wt

city: Utrecht

first: Silja

middle: Barbara

last: Burkhard

address: Uppsalalaan 8

department: Cardiac Development and Genetics

country: Netherlands

institute: Hubrecht Institute

description: heart sample sectioned in 40 individual sections (in columns) IMPORTANT NOTE: sections #17 to #24 were accidentally inverted during tissue processing. This has to be fixed by reordering the sections before processing the data file (correct order: 1-16, 24-17, 25-96)

data_processing:
Paired end reads were aligned to the transcriptome using bwa (version 0.6.2) with default parameters.
The zebrafish transcriptome was based on genome release zv9 and contained improved gene annotations as described in Junker et al. (2014) Cell.
The right mate of each read pair was mapped to the ensemble of all transcripts and to the set of 92 ERCC spike-ins in sense direction. Reads mapping equally to multiple loci were discarded.
Mapped reads were assigned to sections based on barcodes according to the CEL-seq protocol (Hashimshony et al., Cell Reports, 2012)
processed data files contain transcript read counts normalized against spike-in read count and total read count per section
Genome_build: zv9 (with improved 3' annotation, see Junker et al (2014) Cell)
Supplementary_files_format_and_content: csv file containing transcript counts per gene (rows) and section (columns)

source_name: isolated embryonic heart

extract_protocol:
Total RNA was isolated by TRIzol extraction. mRNA was barcoded and amplified using the CEL-seq protocol (Hashimshony et al., Cell Reports, 2012)  see also: Junker et al., 2014, Cell
CEL-seq protocol (Hashimshony et al., Cell Reports, 2012); also  see: Junker et al., 2014, Cell

treatment_protocol: Unfixed embryonic hearts were dissected and embedded in tissue freezing medium. Blocks were cryosectioned, and individual sections were transferred to Eppendorf tubes on dry ice. For extended experimental procedure see also Junker et al. (2014) Cell.

value: embryonic heart

tag: developmental stage

value: 48 hours post fertilization

tag: section direction

value: anterior/outflow tract to posterior/inflow tract; located in sections #2 - #41

tag: section thickness

value: 10µm sections


title: heart2_2dpf_wt

description: heart sample sectioned in 48 individual sections (in columns)

value: posterior/inflow tract to anterior/outflow tract; located in sections #49 - #96


title: Col-0_R1

city: Beijing

first: Lei

address: Nanxincun 20, Xiangshan, Haidian District

department: Key Laboratory of Plant Molecular Physiology

institute: insitute of botany, chinese academy of sciences

data_processing:
The clustering of the index-coded samples was performed on a cBot cluster generation system using HiSeq SR Cluster Kit v4-cBot-HS (Illumina) according to the manufacturer’s instructions. After cluster generation, the libraries were sequenced on an Illumina Hiseq 2500 platform and 50 bp single-read reads were generated.
Raw Data was processed with Perl scripts to ensure the quality of data used in further analysis. The adopted filtering criteria are as follows：1) Remove the adaptor-polluted reads (Reads containing more than 5 adapter-polluted bases were regarded as adaptor-polluted reads and would be filtered out); 2) Remove the low-quality reads. Reads with the number of low quality bases (phred Quality value less than 19) accounting for more than 15% of total bases are regarded as low-quality reads; 3) Remove reads with number of N bases accounting for more than 5 %. As for paired-end sequencing data, both reads would be filtered out if any read of the paired-end reads are adaptor-polluted. The obtained clean data after filtering will be carried out on statistics analyses on its quantity and quality, including Q30, data quantity and base content statistics, etc. Then high quality reads were aligned against the Arabidopsis thaliana reference genome sequence (TAIR10 Genome Release) with TopHat (version 2.0.12).
Genome_build: TAIR10
Supplementary_files_format_and_content: tab-delimited text files include RPKM values for each Sample

organism: Arabidopsis thaliana

source_name: Arabidopsis whole seedling

growth_protocol: Plants were grown under LD conditions at 22ºC for 10 days and harvest at ZT12.

extract_protocol:
Total RNA was extracted with Trizol reagent (Ambion) and treated with DNase I (Thermo Fisher).
Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (#E7530L, NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample.

tag: time point

tag: medium

value: MS+3% sucrose

tag: genotype

value: Wild type

tag: ecotype


title: lux-6_R2

value: lux -/-


title: lux-6_R1


title: Col-0_R2


title: RAW.SWNT/CpG.2

city: Duarte

first: Jacob

middle: M

last: Berlin

state: California

address: 1500 E Duarte Rd

institute: City of Hope

description: RNA-Seq data, RAW-Blue, SWNT/CpG treatment, replicate 2

data_processing:
Illumina Casava1.8 software was used for basecalling
Reads aligned using TopHat2 (v2.0.14), with --no-coverage-search --library-type fr-firststrand
Unique reads quantified using htseq-count (version 0.6.0), with database generated from TxDb.Hsapiens.UCSC.mm9.knownGene (Bioconductor annotations, downloaded 6/8/2016)
Genome_build: mm9
Supplementary_files_format_and_content: RPKM normalized expression (using total aligned reads and TxDb annotations in R) and read counts (calculated using htseq-count)

molecule: polyA RNA

growth_protocol: The K-Luc murine glioma cell line (C57BL/6 origin, luciferase-expressing KR158B cells [K-Luc]) was generous gift from Dr. John Sampson. RAW-Blue cells were purchased from InvivoGen. RAW-Blue cells are RAW 264.7 murine macrophages with a stably integrated SEAP reporter for NF-κB and AP-1. K-Luc and RAW-Blue cells were cultured and maintained in DMEM (gibco, +4.5 g/L D-glucose, +L-glutamine, +110 mg/L sodium pyruvate) supplemented with 10% FBS, (BioWhittaker), 100 U/mL penicillin-G, 100 µg/mL streptomycin and 0.01 M HEPES buffer (Life Technologies).

extract_protocol:
At the conclusion of the migration assay, the supernatant was removed, the cells were rinsed with RNase-free 1xPBS, and immediately lysed with TRIzol Reagent (1 mL for K-Luc and 0.25 mL for RAW-Blue). The TRIzol cell lysate was stored at -80 ºC until RNA extraction. RNA extraction was performed according the manufacturer’s instructions with an additional centrifugation step before adding chloroform in order to pellet the SWNTs (per manufacturer’s instructions for “samples with high content of fat, proteins, polysaccharides, or extracellular material”). Typical total RNA yields were ~20 μg (K-Luc) and 5-10 μg (RAW-Blue). The RNA was stored at -80 ºC in molecular biology grade deionized water until library preparation.
KAPA Stranded mRNA-Seq Kit (Kapa Biosystems, KK8421)

treatment_protocol: A migration assay was performed using a 2-well culture insert in a 35 mm μ-dish (ibidi). For K-Luc, cells were plated both inside the insert chambers (10,000 cells per chamber or 45,000-50,000 cells/cm2) and in the dish outside of the insert (300,000 cells or 45,000-50,000 cells/cm2). For RAW-Blue, cells were plated only inside the chambers (10,000 cells per chamber or 45,000-50,000 cells/cm2). After adhering for 24 h, the cells were rinsed and treated. Cells were then incubated in the migration assay with the treatment for 17-19 h (K-Luc) or 47-50 h (RAW-Blue). The treatment groups were:  untreated control, CpG, SWNT/LP, CpG + SWNT/LP, and SWNT/CpG. Cells in the “untreated control” group were treated with sterile Milli-Q grade water (40 μL water in 2 mL media). The “CpG-only” group received CpG-28 (final concentration of 10 μg/mL). The “SWNT/LP-only” group was treated with SWNT/LP, single-walled carbon nanotubes (SWNTs) dispersed in a lipid-PEG construct (final concentration of 10 μg/mL SWNTs and 10 μg/mL Lipid-PEG2000-OMe). The “CpG+SWNT/LP co-treatment” group was treated with both CpG-28 and SWNT/LP (final concentration of 10 μg/mL CpG, 10 μg/mL SWNTs, and 10 μg/mL Lipid-PEG2000-OMe). The “SWNT/CpG” group was treated with SWNTs dispersed in CpG-28 (final concentration of 10 μg/mL SWNTs and 10 μg/mL CpG-28).

tag: cell line

value: RAW-Blue

tag: treatment

value: SWNT/CpG

tag: run


title: RAW.SWNT/CpG.1

description: RNA-Seq data, RAW-Blue, SWNT/CpG treatment, replicate 1


title: RAW.SWNT.2

description: RNA-Seq data, RAW-Blue, SWNT/LP treatment, replicate 2

value: SWNT/LP


title: RAW.SWNT.1

description: RNA-Seq data, RAW-Blue, SWNT/LP treatment, replicate 1


title: MCF7-N small RNA-seq

city: La Jolla

first: Max

last: Chang

state: CA

address: 9500 Gilman Drive

institute: University of California, San Diego

data_processing:
mapping with Bowtie
read counting with Homer
Genome_build: hg19
Supplementary_files_format_and_content: raw count CSV

organism: Homo sapiens

source_name: Normoxic MCF7 breast cancer cells

growth_protocol: culture in DMEM medium

extract_protocol:
Trizol based RNA extraction method
Small RNA libraries were constructed using the NEBNext Small RNA Library prep kit.

treatment_protocol: 1% hypoxia treatment for 18 hrs

tag: cell type

value: epithelial


title: BTIC small RNA-seq

source_name: MCF7 derived breast tumor initiating cell


title: MCF7-H small RNA-seq

source_name: hypoxic MCF7 breast cancer cells


title: MCF7-HIF-1 small RNA-seq

source_name: MCF7 HIF-1 alpha overexpressing cells


title: RAW.CpG+SWNT.2

description: RNA-Seq data, RAW-Blue, CpG+SWNT/LP treatment, replicate 2

value: CpG+SWNT/LP


title: 20140428_C05 E10.5 NR5A1-eGFP single-cell

description:
20140428_C05
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt

tag: strain/background

value: Mixed background

tag: genotype/variation

value: NR5A1-eGFP positive cells

tag: age

tag: c1 ifc size

value: small

tag: sequencing depth

value: 25M reads 100bp p-e


title: RAW.CpG+SWNT.1

description: RNA-Seq data, RAW-Blue, CpG+SWNT/LP treatment, replicate 1


title: RAW.CpG.2

description: RNA-Seq data, RAW-Blue, CpG treatment, replicate 2

source_name: RAW-Blue, CpG treatment, replicate 2

value: CpG


title: heart3_2dpf_wt

description: heart sample sectioned in 29 individual sections (in columns)

tag: tissue

value: embryonic heart

value: 48 hours post fertilization

value: posterior/inflow tract to anterior/outflow tract; located in sections #11 - #39


title: 20140428_C03 E10.5 NR5A1-eGFP single-cell

description:
20140428_C03
Poly-A RNA
Processed data file: XY_NR5A1-eGFP_single-cell_fetal_gonads.txt


title: RAW.CpG.1

description: RNA-Seq data, RAW-Blue, CpG treatment, replicate 1

source_name: RAW-Blue, CpG treatment, replicate 1


title: KLuc.SWNT/CpG.3

description: RNA-Seq data, K-Luc, SWNT/CpG treatment, replicate 3

source_name: K-Luc, SWNT/CpG treatment, replicate 3

value: K-Luc


title: RAW.Untreated.1

description: RNA-Seq data, RAW-Blue, Untreated Control treatment, replicate 1

value: Untreated Control


title: RAW.Untreated.2

description: RNA-Seq data, RAW-Blue, Untreated Control treatment, replicate 2


title: RS095-1-4pm

city: Maastricht

first: Julian

last: Krauskopf

address: Universiteitssingel 50

institute: Maastricht University

description:
processed_data.txt
plasma circulating miRNAs

data_processing:
data processing miRge pipeline v1
mapptin to miRBase release 21
data were transformed to logCPM using the limma package

source_name: plasma

extract_protocol:
600 ul plasma using Qiagen miRNeasy plasma/serum kit
Illumina Truseq small RNA prep kit

tag: dose level

value: H


title: RS016-1-4pm

value: O


title: RS036-1-4pm


title: KLuc.SWNT/CpG.1

description: RNA-Seq data, K-Luc, SWNT/CpG treatment, replicate 1

source_name: K-Luc, SWNT/CpG treatment, replicate 1


title: RS075-1-4pm


title: KLuc.SWNT.3

description: RNA-Seq data, K-Luc, SWNT/LP treatment, replicate 3

source_name: K-Luc, SWNT/LP treatment, replicate 3


title: RS027-2-4pm


title: KLuc.SWNT.2

description: RNA-Seq data, K-Luc, SWNT/LP treatment, replicate 2

source_name: K-Luc, SWNT/LP treatment, replicate 2


title: RS063-1-4pm


title: KLuc.SWNT.1

description: RNA-Seq data, K-Luc, SWNT/LP treatment, replicate 1

source_name: K-Luc, SWNT/LP treatment, replicate 1


title: KLuc.CpG+SWNT.3

description: RNA-Seq data, K-Luc, CpG+SWNT/LP treatment, replicate 3


title: RS013-1-4pm


title: RS060-2-4pm


title: RS020-2-4pm


title: KLuc.CpG+SWNT.2

description: RNA-Seq data, K-Luc, CpG+SWNT/LP treatment, replicate 2


title: KLuc.CpG+SWNT.1

description: RNA-Seq data, K-Luc, CpG+SWNT/LP treatment, replicate 1


title: RS039-2-4pm


title: KLuc.CpG.3

description: RNA-Seq data, K-Luc, CpG treatment, replicate 3

source_name: K-Luc, CpG treatment, replicate 3


title: RS072-2-4pm


title: RS052-2-4pm


title: KLuc.CpG.1

description: RNA-Seq data, K-Luc, CpG treatment, replicate 1

source_name: K-Luc, CpG treatment, replicate 1


title: KLuc.CpG.2

description: RNA-Seq data, K-Luc, CpG treatment, replicate 2

source_name: K-Luc, CpG treatment, replicate 2


title: KLuc.Untreated.1

description: RNA-Seq data, K-Luc, Untreated Control treatment, replicate 1

source_name: K-Luc, Untreated control treatment, replicate 1


title: KLuc.SWNT/CpG.2

description: RNA-Seq data, K-Luc, SWNT/CpG treatment, replicate 2

source_name: K-Luc, SWNT/CpG treatment, replicate 2


title: RS030-2-4pm


title: RS046-2-4pm


title: RS013-2-4pm


title: RS071-1-4pm


title: RS015-1-4pm


title: RS047-2-4pm


title: RS057-1-4pm


title: RS057-2-4pm


title: RS086-2-4pm


title: RS064-1-4pm


title: RS075-2-4pm


title: RS071-2-4pm


title: RS052-1-4pm


title: RS039-1-4pm


title: RS056-1-4pm


title: RS072-1-4pm


title: KLuc.Untreated.2

description: RNA-Seq data, K-Luc, Untreated Control treatment, replicate 2


title: RS042-2-4pm


title: RS020-1-4pm


title: RS035-1-4pm


title: RS046-1-4pm


title: RS056-2-4pm


title: RS016-2-4pm


title: RS044-1-4pm


title: RS064-2-4pm


title: RS035-2-4pm


title: RS047-1-4pm


title: RS036-2-4pm


title: cardiacFibro-SFM1_RNAseq

city: Haifa

first: Izhak

last: Kehat

address: Efron St.

institute: Technion - Israel's Institute of Technology

data_processing:
Reads containing Illumina adapters were trimmed using cutadapt.
TopHat (v2.0.10) was used to align the reads to the rat genome (Rnor 6.0, from Ensembl)
Counting reads on genes (Ensembl release 83) was done with HTSeq-count (version 0.6.1p1)
Differential expression analysis was performed using DESeq2 (1.6.3)
For expression level comparison, DEseq2 counts were normalized to exon length.
Genome_build: Rn6
Supplementary_files_format_and_content: abundance measurements (for all samples)

organism: Rattus norvegicus

source_name: cardiac fibroblasts (heart tissue)

growth_protocol: Neonatal rat ventricular cardiomyocytes and cardiac fibroblasts were obtained from 1-3 days old rats using an enzymatic neonatal cardiomyocyte isolation system (Worthington Biochemical Corp). We used a Percoll (sigma) density centrifugation, to isolate cardiomyocytes and fibroblasts fractions. 1.5-2X106 live cardiomyocytes were plated on 10cm dishes, while fibroblasts were plated without counting.

extract_protocol:
RNA was extracted from isolated cardiomyocytes and cardiac fibroblasts by NucleoSpin RNA kit (Macherey-Negal)
1000ng of total RNA was processed using the TruSeq RNA Sample Preparation Kit v2 protocol (Illumina, Part # 15026495). Sequencing libraries were constructed with barcodes to allow multiplexing of 10 samples on one lane

treatment_protocol: Cell culture meduim was replaced 24h after plating to serum free medium for 'cardioSFM' and 'cardiacFibro-SFM',or to serum free medium + phenylephrine for 'cardiacPE' and 'cardiacFibro-PE'

value: cardiac fibroblasts

tag: passage

tag: growth medium

value: serum free medium


title: cardiacFibro-SFM2_RNAseq


title: cardiacFibro-PE2_RNAseq

value: serum free medium with phenylephrine  (10µM)


title: RS095-2-4pm


title: RS063-2-4pm


title: GSC-827-NSC-2

city: Seattle

first: Sonali

last: Arora

state: WA

address: 1100 Fairview Ave N,

institute: FHCRC

description:
GSC-0827 replicate 2
827-NSC-2

data_processing:
Sequencing was performed using an Illumina HiSeq 2000 in Rapid Run mode and  employed a paired-end, 50 base read length (PE50) sequencing strategy.
RNA-seq reads were aligned to the UCSC hg19 assembly using Tophat2 and counted for gene associations against the UCSC genes database with HTSeq
All data was combined and normalized using a CPM method from the R/Bioconductor package, edgeR
Genome_build: hg19
Supplementary_files_format_and_content: tab-delimited text files include cpm values for each Sample.

extract_protocol:
RNA was extracted from cell cultures every two weeks during the exponential growth phase; RNA was isolated from 0.5-1.0×106 cells. Total RNA was prepared using RNeasy Mini Kit with DNase I (Qiagen). RNA concentration and quality (A260/A280) was measured using a NanoDrop 1000 Spectrophotometer (Thermo Scientific)
RNA libraries were prepared for sequencing using standard Illumina protocols

value: GBM stem-like cells (GSCs)

tag: shRNA

value: shControl


title: cardiacFibro-PE1_RNAseq


title: RS015-2-4pm


title: RS042-1-4pm


title: GSC-827-NSC-1

description:
GSC-0827 replicate 1
827-NSC-1


title: cardioSFM3_RNAseq

source_name: cardiomyocytes (heart tissue)

value: neonatal rat ventricular myocytes


title: RS027-1-4pm


title: GSC-827-478-2

description:
GSC-0827 replicate 2
827-478-2


title: cardioSFM2_RNAseq


title: GSC-827-478-1

description:
GSC-0827 replicate 1
827-478-1


title: GSC-166-NSC-2

description:
GSC-0166 replicate 2
166-NSC-2


title: cardioPE3_RNAseq

value: serum free medium with phenylephrine (10µM)


title: RS030-1-4pm


title: RS044-2-4pm


title: MCF control RNA-seq

data_processing:
mapping with STAR
read counting and normalization with Homer
Genome_build: hg19
Supplementary_files_format_and_content: normalized read count CSV

source_name: MCF7 cells overexpressing control vector

extract_protocol:
Trizol based RNA extraction method
RNA libraries were prepared for sequencing using standard Illumina protocols.

treatment_protocol: no specific treatment applied

value: epithelial


title: GSC-166-NSC-1

description:
GSC-0166 replicate 1
166-NSC-1


title: RS060-1-4pm


title: cardioPE1_RNAseq


title: cardioPE2_RNAseq


title: MCF miR25/93 RNA-seq

source_name: MCF7 cells overexpressing miR25/93


title: input_cardiacFibro2_ChIPseq

library_source: genomic

data_processing:
Adapters were removed from all samples using the cutadapt tool
Reads that had a length less than 40 bases after the adapter trimming were discarded (cutadapt option –m 40).
Reads for each sample were mapped independently using Bowtie version (v 0.12.9) against the Rattus norvegicus genome.  Genome assembly: Rnor_6.0, Ensembl release 83
Peaks were called using Macs2 callpeak --nomodel --extsize 146.
Genome_build: Rn6
Supplementary_files_format_and_content: Bedgraph. In each file, peaks present in all biological replicates ('common peaks')

molecule: genomic DNA

extract_protocol:
according to MAGnify™ Chromatin Immunoprecipitation System (Invitrogen) protocol.
Libraries were prepared according to Blecher-Gonen et al.2013. Briefly, the ChIP product was subjected to end repair, A base addition, adapter ligation and PCR amplification to create libraries. The adaptors contain different barcodes to allow multiplexing of 12 samples in one pool.

treatment_protocol: Cell culture meduim was replaced 24h after plating to serum free medium for 'cardioSFM',or to serum free medium + phenylephrine for 'cardiacPE'

value: serum containing medium

tag: chip antibody


title: GSC-166-478-2

description:
GSC-0166 replicate 2
166-478-2


title: GSC-131-NSC-2

description:
GSC-0131 replicate 2
131-NSC-2


title: input_cardiacFibro1_ChIPseq


title: GSC-131-NSC-1

description:
GSC-0131 replicate 1
131-NSC-1


title: input_cardioSFM2_ChIPseq


title: GSC-131-478-2

description:
GSC-0131 replicate2
131-478-2


title: input_cardioSFM1_ChIPseq


title: GSC-131-478-1

description:
GSC-0131 replicate1
131-478-1


title: cardioSFM1_RNAseq


title: RS086-1-4pm


title: input_cardioPE2_ChIPseq


title: 12h_III

city: Ribeirão Preto

first: Pablo

middle: Rodrigo

last: Sanches

state: SP

address: Av. Bandeirantes, 3900

department: Genetics

country: Brazil

institute: University of São Paulo

description:
geneCounts_12h.txt
DESeq_12h_vs_0h.csv
DESeq_12h_vs_3h.csv

data_processing:
FastQC v0.11.2 was used to visualize the libraries quality before and after trimming.
FASTX-Tookit (fastq_quality_filter) was used to remove poor quality sequences, such that remaining sequences had a minimum quality score of 20 with a minimum of 50% of bases within a read meeting this quality score requirement.
Filtered reads were mapped onto the T. rubrum genome using the Bowtie2 v2.1.0.
Reads were assigned to annotated genes and counted using the "summarizeOverlaps" function from the GenomicRanges Bioconductor package.
The data were analyzed using the R package DESeq version 1.28.0. The samples were normalized, and the dispersions were estimated using the default DESeq settings. DESeq was then used to identify differentially expressed genes for each pairwise comparison of interest. Genes with an adjusted P value of < 0.05 and a log2(foldchange) > 1.5 (upregulation) or < -1.5 (downregulation) were called as differentially expressed.
Genome_build: Broad T. rubrum strain CBS118892 genome
Supplementary_files_format_and_content: tab-delimited text files include reads count values for each sample and DESeq output in CSV format containing mean expression, fold changes and p value.

organism: Trichophyton rubrum

source_name: CBS 118892 - UDA - 12 hours, replicate III

growth_protocol: T. rubrum conidia (about 1x10^6 conidia per mL) were grown on Sabouraud medium for 96h, at 28ºC under shaking.

extract_protocol:
Approximately 100 mg of frozen mycelia were used for total RNA extraction using  Illustra RNAspin Mini Isolation Kit (GE, USA)  and treated with RNAse-free DNAse I. RNA concentrations were estimated through NanoDrop ND-1000 spectrophotometer, and RNA integrity was verified using  Agilent Bioanalyser platform 2100.
RNA libraries were prepared for sequencing according to Illumina guidelines.

treatment_protocol: The resulted mycelium  from pre inoculum of T. rubrum conidia was transferred to RPMI 1640 medium in the absence or presence UDA (17.25 μg/mL) for 3h and 12h under shaking.

tag: time point


title: input_cardioPE1_ChIPseq


title: 12h_II

source_name: CBS 118892 - UDA - 12 hours, replicate II


title: H3_cardiacFibro2_ChIPseq

value: anti H3K27ac antibody (abcam ab4729).


title: 12h_I

source_name: CBS 118892 - UDA - 12 hours, replicate I


title: 3h_III

description:
geneCounts_3h.txt
DESeq_3h_vs_0h.csv
DESeq_12h_vs_3h.csv

source_name: CBS 118892 - UDA - 3 hours, replicate III


title: H3_cardioSFM2_ChIPseq


title: H3_cardiacFibro1_ChIPseq


title: 3h_II

source_name: CBS 118892 - UDA - 3 hours, replicate II


title: 3h_I

source_name: CBS 118892 - UDA - 3 hours, replicate I


title: H3_cardioPE1_ChIPseq


title: 0h_II

description:
geneCounts_0h.txt
DESeq_3h_vs_0h.csv
DESeq_12h_vs_0h.csv

source_name: CBS 118892 - control, replicate II


title: H3_cardioSFM1_ChIPseq


title: GSC-166-478-1

description:
GSC-0166 replicate 1
166-478-1


title: cardiacFibro2_ATACseq

data_processing:
Reads containing Nextera transposase or RNA PCR primer were trimmed using cutadapt
The reads were aligned uniquely to the rat genome (Rnor 6.0, from Ensembl) using bowtie (version 1.0.0)
Mitochondrial reads were filtered, and duplicated reads were removed using Picard tools
The two biological replicates were correlated and combined
Peaks were called using Macs2 callpeak --nomodel --extsize 100 --shift -46
Genome_build: Rn6
Supplementary_files_format_and_content: Bedgraph. In each file- peaks present in all biological replicates ('common peaks')

extract_protocol:
25000 cells from each sample were centrifugated and resuspended with lysis buffer, followed by transposition reaction using Tn5 transposase (Nextera kit).
Nextera DNA preparation kit  Illumina,Catalog number-FC-121-1030

treatment_protocol: Cell culture meduim was replaced 24h after plating to serum free medium for 'cardioSFM', serum free medium + phenylephrine for 'cardiacPE', serum free medium + infection with adenovirus expressing WT GATA4 gene for 'cardioGata4'


title: 0h_III

source_name: CBS 118892 - control, replicate III


title: cardioGata4_2_ATACseq

value: serum free medium. Cells were tranduced with adenovirus encoding for Gata4-gene


title: cardioGata4_1_ATACseq


title: cardioSFM2_ATACseq


title: cardioSFM1_ATACseq


title: cardioPE2_ATACseq


title: cardioPE1_ATACseq


title: SLE-peli1-high1

status: Public on Feb 05 2018

city: 上海

first: Junli

last: Liu

address: New Research Buliding,320 Yue Yang Road Shanghai, P.R.China

institute: Institute of Health Sciences   Shanghai Institutes for Biological Sciences   Chinese Academy of Sciences

data_processing:
Transcriptomic reads from the RNA-Seq experiments were mapped to a reference genome (mm10) using Bowtie.
Gene expression levels were quantified using the RSEM software package.
Differentially expressed genes were subsequently analyzed using the DAVID bioinformatics platformand IPA.
Genome_build: hg38
Supplementary_files_format_and_content: Processed data were txt format and all gene FPKM of each sample were contained in each txt file.

source_name: SLE-peli1-high_peripheral blood

extract_protocol:
PBMCs were isolated from Peripheral blood，and RNA was harvested using Trizol reagent.
RNA libraries were prepared for sequencing using standard Illumina protocols

tag: subject status

value: SLE patient

tag: peli1 expression level

value: high

value: Peripheral blood


title: SLE-peli1-high2


title: SLE-peli1-low1

source_name: SLE-peli1-low_peripheral blood

value: low


title: SLE-peli1-low2


title: NC2

source_name: Health donor_peripheral blood

value: Health donor


title: NC1


title: WT_10h (2)

city: Basel

first: Sarah

last: Carl

address: Maulbeerstrasse 66

department: Epigenetics

country: Switzerland

institute: Friedrich Miescher Institute

description:
10 hour after temperature shift
xrn2cd_downstream_upstream_counts.tab
xrn2cd_gene_counts.tab

data_processing:
Basecalling was performed using RTA v. 1.13.48.
Reads were aligned to the ce10 (WS220) genome assembly using the qAlign function from the QuasR R package (v. 3.2). ChIP-seq reads were aligned using the default parameters, while RNA-seq reads were aligned using the parameter “splicedAlignment=TRUE”, resulting in splice-aware alignments.
Gene expression was quantified using WS220 annotations downloaded from WormBase. The qCount function in the QuasR package was used to count reads overlapping all annotated exons for each gene.
Genome_build: ce10 (WS220)
Supplementary_files_format_and_content: Tab-delimited file: First column contains gene names (WormBase identifiers), following columns contain raw counts of reads overlapping each gene or the dowstream/upstream regions for each sample. Downstream regions extend 10kb from the 3’ end of the gene or until the next gene on the same strand; upstream regions extend 2kb from the 5’ end of the gene. BigWig: counts of the 5’-end of reads, summed in 50-bp bins across the genome. BigWig files from the same experiment are normalized for sequencing library depth within that experiment.

organism: Caenorhabditis elegans

source_name: Whole worms

extract_protocol:
RNA was extracted using TRI Reagent (Molecular Research Center) and treated with DNase.
Library was prepared using the TruSeq Standard mRNA Library Prep Kit (Illumina).

treatment_protocol: Worms were cultured from L1 to early L4 stage for 59 hours at 15°C, and temperature was shifted to 26°C. The worms were further cultured for 10 hours and harvested.

tag: strain/genotype

value: N2 (wild-type)

tag: developmental stage


title: xrn-2cd_xrn-2(xe31)_10h