title: IMIM-PC2 Resistant Rep2

city: Madrid

first: Victor Javier

last: Sanchez-Arevalo Lobo

address: Melchor Fernandez Almagro, 3

department: Cancer Biology

institute: CNIO

data_processing:
Image analysis and per-cycle basecalling was performed with Illumina Real Time Analysis software (RTA1.13). Conversion to FASTQ read format was performed with CASAVA-1.8 (Illumina).
Quality check was done via fastqc (v0.9.4, Babraham Bioinformatics). The raw reads were aligned to the reference genome hg19/GRCh37 with tophat1 (version 2.0.4) using the following parameters: -- bowtie1, --max-multihits 5, --genome-read-mismatches 1, --segment-mismatches 1, -- segment-length 19, --splice-mismatches 0, --library-type fr-firststrand.
The gene expression levels (Fragments Per Kilobase of exon per Million fragments, FPKMs) were quantified with cufflinks2 (version 2.0.2), as annotated in Ensembl version GRCh37.65, with the following parameters: -N, --library-type fr-firststrand, -u.
Genome_build: hg19
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample ...

source_name: Cell line

growth_protocol: Cells were cultured in DMEM (Sigma- Aldrich) supplemented with 10% heat-inactivated fetal bovine serum (FBS), 1% Na pyruvate and 1% penicillin/streptomycin under standard conditions.

extract_protocol:
RNA from parental and MN58b-resistant cells was extracted and purified using Trizol, RNA integrity was assayed on an Agilent 2100 Bioanalyzer (range 8.4-10). PolyA+ fractions were purified and randomly fragmented, converted to double stranded cDNA and processed through subsequent enzymatic treatments of end-repair, dA-tailing, and ligation to adapters as in Illumina's "TruSeq Stranded mRNA Sample Preparation Part # 15031047 Rev. D". Adapter- ligated library was completed by 10 cycles of PCR with Illumina PE primers. The resulting purified cDNA library of template molecules was applied to an Illumina flow cell for cluster generation (TruSeq cluster generation kit v5) and sequenced on the Genome Analyzer IIx (GAIIx) with SBS TruSeq v5 reagents following manufacturer's instructions (SingleRead 1x40 bases). Image analysis and per-cycle base calling was performed with Illumina Real Time Analysis software (RTA1.13
RNA libraries were prepared for sequencing using standard Illumina protocols

treatment_protocol: To generate MN58b-resistant IMIM-PC-2 cells, MN58b was added starting at a 0.1μM. In parallel, IMIM-PC-2 cells were cultured without MN58b. Medium was changed every 48 hours and MN58b concentration was increased by 50% weekly at each passage of the cells (split 1:3 when confluent). The final concentration achieved was 8μM.

tag: phenotype

value: MN58b Resistant


title: IMIM-PC2 WT Rep1

status: Public on Aug 11 2017

type: SRA

country: Spain

library_source: transcriptomic

molecule: total RNA

organism: Homo sapiens

tag: cell line

value: MN58b Sensitive


title: IMIM-PC2 Resistant Rep1


title: IMIM-PC2 WT Rep3


title: IMIM-PC2 Resistant Rep3


title: AD_031_5

city: Freiburg im Breisgau

first: Galina

last: Erikson

address: Stübeweg 51

country: Germany

institute: Max Planck Institute of Immunobiology and Epigenetics

description:
Processed data file: raw.txt
Processed data file: fpkm.txt

data_processing:
Sequenced reads were quality tested using FASTQC v0.11.2 using the default parameters.
Alignment to the mm10 genome was performed using the STAR aligner version 2.4.0k using the --outFilterIntronMotifs RemoveNoncanonical parameter.
Gene expression was quantified using the HOMER analysis suite and differential gene expression was carried out using the edgeR package v3.6.8 using duplicates to copute within-group dispersion. FDR < 0.05 and log2 fold change greater than 0.5.
Genome_build: mm10 (GRCm38)
Supplementary_files_format_and_content: raw.txt: Tab-delimited text file includes raw counts.
Supplementary_files_format_and_content: fpkm.txt: Tab-delimited text file includes fpkm normalized gene expression table.

organism: Mus musculus

source_name: Hippocampus, AD, CAD-31

extract_protocol:
RNA was isolated from hippocampus using the RNeasy Plus Universal mini kit (Qiagen).
RNA-Seq libraries were prepared using the Illumina TruSeq Stranded mRNA Sample Prep Kit according to the manufacturer’s instructions.

tag: strain/background

tag: genotype/variation

tag: tissue

value: hippocampus

tag: drug treatment


title: AD_6

source_name: Hippocampus, AD, vehicle

value: Vehicle


title: AD_3


title: WT_031_3

source_name: Hippocampus, WT, CAD-31

value: wild-type


title: WT_4

source_name: Hippocampus, WT, vehicle


title: WT_2


title: WT_5


title: AD_1


title: WT_031_2


title: WT_1


title: Wild type rep 2

city: New York

first: Rajal

last: Sharma

state: NY

address: 1425 Madison Ave., Room 16-52

country: USA

institute: ISMMS

description: Rep 2

data_processing:
Illumina Casava software was used for basecalling.
Reads were filtered using Trimmomatic and then aligned to the mouse reference genome (mm9) using Tophat v2.1.0
HT-seq was used to find read counts across the reference genome
DESeq2 was used to find differentially expressed genes with FDR < 0.1 and fold-change > 1.5
Genome_build: mm9
Supplementary_files_format_and_content: Tab-delimited text file of normalized counts for all samples

source_name: Mouse embryonic stem cells

growth_protocol: Wild-type (WT), Thap1 C54Y/C54Y and Thap1 ΔExon2 ESCs were maintained on 0.1% gelatin-coated tissue culture plates on irradiated mouse embryonic fibroblasts (MEFs) in DMEM containing 15% fetal calf serum, 0.1 mM 2-mercaptoethanol, L-glutamine, nonessential amino acids, and leukemia inhibitory factor (LIF) (1000 units/ml) and penicillin/streptomycin (ThermoFisher).

extract_protocol:
Extracted using the RNeasy kit (Qiagen)
Constructed using the TrueSeq RNA sample preparation kit (Illumina RS-122- 2001) as per manufacturer’s recommendations.

tag: cell type

value: ES cells derived from C57Bl/6 blastocysts

tag: genotype

value: WT

tag: strain


title: AD_031_3


title: THAP1 C54Y rep 2

description: Rep 2

value: THAP1 C54Y


title: IMIM-PC2 WT Rep2


title: AD_031_6


title: Med12cKO ventricle 1

city: Dallas

first: Kedryn

middle: K

last: Baskin

state: TX

address: 6000 Harry Hines

department: Molecular Biology

institute: UT Southwestern

data_processing:
Quality filtered reads were aligned to the mouse reference genome GRCm38 (mm10) using the Tophat2 (v 2.0.0) aligner with default settings (67).
The R package edgeR (v 3.14.0) was used for differential gene expression analysis.
Read counts obtained from featureCounts were normalized by taking the median of each gene count across samples and dividing each sample gene count by the relative ratio of library sizes between the calculated median and sample size.
The average normalized expressions values of the samples were used to calculate fold change and P values. Cutoff values of fold change greater than 1.5 and P < 0.05 were then used to select for differentially expressed genes between sample group comparisons.
Genome_build: GRCm38 (mm10)

source_name: heart ventricles from 1-day old mouse

extract_protocol: Total RNA was isolated from both ventricles of male P1 CTL and Med12cko mice using TRIzol™ according to the manufacturer's instructions.

tag: age

value: Heart ventricles

value: Med12cKO (CrePOS)


title: THAP1 -/- rep 1

description: Rep 1

value: THAP1 -/-


title: Wild type rep 1

description: Rep 1


title: Med12cKO ventricle 2


title: Med12cKO ventricle 3


title: CTL ventricle 1

value: Control (CreNEG)


title: transgenic 1

city: Worcester

first: Yongxu

last: Wang

state: MA

address: 364 Plantation Street

department: Program in Gene Function and Expression

institute: UMass Medical School

data_processing:
Images were analyzed using OLB 1.9 and CASAVA_v1.8.2
TopHat v2.0.9 (bowtie2/2.1.0) was used to map the sequences to mouse genome mm10
mapped reads were processed by cufflinks (v2.2.1)
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text file including FPKM for each gene output from cummeRbund

source_name: TG, subcutaneous inguinal white fat

growth_protocol: 12-week--old male mice

extract_protocol:
RNA was extracted using Trizol.
Illumina TruSeq RNA sample Preparation V2 guide

tag: gender

value: male

value: 12 weeks old

value: Hlx transgenic

value: subcutaneous inguinal white fat


title: transgenic 3


title: transgenic 2


title: WT_031_1


title: wild type 3

source_name: WT, subcutaneous inguinal white fat


title: THAP1 -/- rep 2

description: Rep 2


title: wild type 2


title: 5dpf lck:GFPPos_rep2

city: Wageningen

first: Julien

last: Rougeot

state: Gerlderland

address: Droevendaalsesteeg 1  Building 107

department: Plant Science Group

country: Netherlands

institute: Wageningen University and Research

description: FACS-sorted

data_processing:
Image analysis and base calling was done by the Illumina HCS version 1.15.1
Quality trimmed reads were aligned to the Ensembl zebrafish genome (Zv9) using Bowtie and reads were mapped to zebrafish transcripts using TopHat and a modified version of the Ensembl Zv9_79 annotation containing few additional genes.
Genome_build: Zv9
Supplementary_files_format_and_content: tab-separated value files include gene counts for each sample

organism: Danio rerio

source_name: 5dpf_lck positive lymphoid cells

growth_protocol: Zebrafish were handled in compliance with the local animal welfare regulations and maintained according to standard protocols (http://ZFIN.org). Embryos were grown at 28,5°C in egg water (60µg/ml Instant Ocean sea salts) containing 0.003% 1-phenyl-2-thiourea (SigmaFor infected samples. Zebrafish embryos were manually dechorionated at 24 hpf and at 28 hpf they were micro-injected into the caudal vein with 125 CFU of M. marinum M strain bacteria suspended in PBS/2%PVP.-Aldrich)

extract_protocol:
Between 200 and 300 larvae per condition were collected at 5 or 6 dpf. Larvae were dissociated into single cell suspension by Trypsin treatment and fluorescent and non-fluorescent cell fraction were subsequently sorted by FACS as described in Rougeot et al., (2014, Methods Mol Biol 1197:41-66). RNA were isolated using the RNAqueous microkit (Ambion) and cDNA were synthetized and amplified with the SMARTer cDNA synthesis kit for Illumina Sequencing (Clonetech) according to the manufacturer’s instructions. The integrity of the RNA and cDNA was confirmed by Lab-on-chip analysis using the 2100 Bioanalyzer (Agilent Technologies).
The sheared amplified cDNA  were used to make RNAseq libraries using the Illumina TruSeq RNA Sample Preparation Kit v2 (Illumina Inc., San Diego, USA). In the manufacturer’s instructions two modifications were made. In the adapter ligation step 1 µl instead of 2.5 µl adapter was used. In the library size selection step the library fragments were isolated with a double Ampure XP purification with a 0.7x beads to library ratio. The resulting mRNA-Seq library was sequenced using an Illumina HiSeq2000 instrument according to the manufacturer’s description with a read length of 2 x 50 nucleotides for the uninfected samples and 1 x 50 nucleotides for the infected samples.

value: zebrafish larvae

tag: developmental stage

value: lck positive lymphoid cells


title: wild type 1


title: 5dpf lck:GFPNeg_rep2

description: FACS-sorted

source_name: 5dpf_lck negative lymphoid cells

treatment_protocol: For infected samples. Zebrafish embryos were manually dechorionated at 24 hpf and at 28 hpf they were micro-injected into the caudal vein with 125 CFU of M. marinum M strain bacteria suspended in PBS/2%PVP.

value: lck negative lymphoid cells


title: 5dpf lck:GFPNeg_rep1

description: FACS-sorted


title: 5dpf lck:GFPPos_rep1

description: FACS-sorted


title: 5dpf mpx:GFPPos_rep2

description: FACS-sorted

source_name: 5dpf_mpx positive neutrophils

value: mpx positive neutrophils


title: CTL ventricle 3


title: 5dpf mpx:GFPNeg_rep1

description: FACS-sorted

source_name: 5dpf_mpx negative neutrophils

value: mpx negative neutrophils


title: 6dpf_mpeg1:mCherryPos_Mm:GFPPos_rep2

description: FACS-sorted

source_name: 6dpf_mpeg1 positive macrophages

tag: caudal vain infection

value: M. marinum M strain, 125cfu

value: mpeg1 positive macrophages


title: THAP1 C54Y rep 1

description: Rep 1

value: ES cells derived from C57Bl/6 blastocysts


title: 6dpf_mpeg1:mCherryPos_Mm:GFPNeg_rep2

description: FACS-sorted


title: CTL ventricle 2


title: 6dpf_mpeg1:mCherryPos_rep2

description: FACS-sorted


title: 6dpf_mpeg1:mCherryNeg_Mm:GFPNeg_rep2

description: FACS-sorted

source_name: 6dpf_mpeg1 negative macrophages

value: mpeg1 negative macrophages


title: 5dpf mpx:GFPNeg_rep2

description: FACS-sorted


title: 5dpf mpx:GFPPos_rep1

description: FACS-sorted


title: 6dpf_mpeg1:mCherryPos_Mm:GFPNeg_rep1

description: FACS-sorted


title: 6dpf_mpeg1:mCherryNeg_rep2

description: FACS-sorted


title: 6dpf_mpeg1:mCherryPos_Mm:GFPPos_rep1

description: FACS-sorted


title: 6dpf_mpeg1:mCherryNeg_Mm:GFPNeg_rep1

description: FACS-sorted


title: 5dpf mpeg1:gal4 ; UAS Kaede Positive_rep2

description: FACS-sorted

source_name: 5dpf_mpeg1 positive macrophages

value: mpeg1:gal4;UAS Kaede Positive


title: 6dpf_mpeg1:mCherryNeg_rep1

description: FACS-sorted


title: 5dpf mpeg1:gal4 ; UAS Kaede Positive_rep1

description: FACS-sorted


title: 5dpf mpeg1:gal4 ; UAS Kaede Negative_rep1

description: FACS-sorted

source_name: 5dpf_mpeg1 negative macrophages

value: mpeg1:gal4;UAS Kaede Negative


title: 5dpf mpeg1:gal4 ; UAS Kaede Negative_rep2

description: FACS-sorted


title: 6dpf mpeg1:gal4 ; UAS Kaede Positive_rep2

description: FACS-sorted


title: 6dpf mpeg1:gal4 ; UAS Kaede Positive_rep1

description: FACS-sorted


title: 6dpf mpeg1:gal4 ; UAS Kaede Negative_rep2

description: FACS-sorted


title: Ulp2D.Slx5D_1

city: New Haven

first: Hong-Yeoul

last: Ryu

state: CT

address: 266 Whitney Ave

department: MB&B

institute: Yale

data_processing:
The fastq reads obtained from the sequencer were processed for adapter removal and trimmed for quality using custom PERL scripts
The sequences were aligned to the reference genome using TopHat version 2.0.12.  The exact command line used was -p 8, --library-type fr-firststrand -G Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.gtf
Cuffdiff was run on the generated aligned files, and transcript abundance estimated.
The cuffdiff output was processed using cummeRbund
The results were visualized using R
Genome_build: Ensemble Saccharomyces cerevisiae R64-1-1 (from the Illumina iGenomes)
Supplementary_files_format_and_content: The fpkm matrix table with values for all the replicates.

molecule: polyA RNA

organism: Saccharomyces cerevisiae

growth_protocol: YPD, 30˚C

extract_protocol:
RNeasy Kit (Qiagen)
oligo(dT) selection-cDNA

value: MHY9109 MATalpha his3delta_1 leu2delta_0 met15delta_0 ura3delta_0 lys2delta_0 ulp2delta_::kanMX slx5delta_::kanMX


title: Set1D_2

value: MHY8915 MATalpha his3delta_1 leu2delta_0 met15delta_0 ura3delta_0 lys2delta_0 set1delta_::HIS3


title: Ulp2D.Slx5D_0


title: Ulp2D.Slx5D_2


title: 6dpf mpeg1:gal4 ; UAS Kaede Negative_rep1

description: FACS-sorted


title: 6dpf_mpeg1:mCherryPos_rep1

description: FACS-sorted


title: Input Mock

city: West Haven

first: Christine

last: Jacobs-Wagner

address: 840 West Campus Drive, PO BOX 27388

department: Molecular, Cellular and Developmental Biology

institute: Yale University

library_source: genomic

data_processing:
The reads from the sequencer were trimmed using FastX Trimmer.
The reads were aligned to the Caulobacter crescentus (NA1000) strain.  This reference strain was downloaded from the NCBI Refseq and indexed using bwa.  The reads were alined to this index using bwa mem
Perbase coverages were calculated using coverageBed program, and these values were further normalized to million mapped reads.
Genome_build: Caulobacter crescentus (NA1000)
Supplementary_files_format_and_content: The processed files are the normalized coverage files in BEDGRAPH format.  The first column denotes the chromosome, the second and their column denote the span, and the last column denotes the normalized coverage per million mapped reads.

molecule: genomic DNA

organism: Caulobacter vibrioides NA1000

source_name: Asynchronous population

growth_protocol: ChIP-seq samples were obtained from mid-log (OD660nm ≈ 0.4) cultures of CJW5534 producing RedN-Venus grown in PYE at 30°C. In parallel, a “mock ChIP” sample was collected from a PYE culture of CJW5796 producing freely diffusing Venus after 1h 30 min of induction with 0.03% xylose.

extract_protocol:
Immunoprecipitated DNA was extracted as described before (Fumeaux et al., 2014) with the following modifications: PierceTM Protein A/G Magnetic Beads (Thermo Fisher Scientific) and anti-GFP (JL-8) Living Colors® Av Monoclonal Antibody (Clontech Laboratories) were used for protein immunoprecipitation, SDS was excluded from ChIP buffer, Herring sperm DNA was not used to saturate beads and sonication was performed using a Digital Sonifier® S-250D on ice with the 1/8” microtip (Branson Sonic Power Co) with 45% output and 10 cycles of 30 s ON/30 s OFF.
Quality and concentration of the ChIP-DNA was assessed by estimating the A260/A280 and A260/A230 ratios with a Nanodrop device (Thermo Scientific).  Library preparation and sequencing was performed by the Yale Center for Genome Analysis as follows: ~10ng of immunoprecipitated DNA was evaluated with a 2100 Bioanalyzer (Agilent Technologies, Santa Clara CA) using a Quant-iT™ DNA Assay Kit high sensitivity (Thermo Fisher Scientific). ChIP-DNA was further purified using Ampure XP SPRI beads (Beckman Coulter Genomics). The DNA was then end-repaired, A-tailed, adapter ligated and PCR enriched (8 -10 cycles). Indexed libraries that met appropriate cut-offs were quantified by both qRT-PCR and insert size distribution determined with the LabChip GX. Samples with a yield of ≥0.5 ng/ul are used for sequencing. Subsequently, for sample concentrations were normalized to 10 nM and loaded into Illumina Rapid or High-output flow cells (Illumina®, San Diego CA) at a concentration that yields 150-250 million passing filter clusters per lane. Samples were sequenced using 75 bp single or paired-end sequencing on an Illumina HiSeq 2500 according to Illumina protocols. The 6 bp index was read during an additional sequencing read that automatically follows the completion of read 1. A positive control (prepared bacteriophage Phi X library) provided by Illumina was spiked into every lane at a concentration of 0.3% to monitor sequencing quality in real time.

treatment_protocol: DNA was cross-linked to proteins with addition of 1% formaldehyde in presence of 7.5mM Sodium phosphate pH 7,6. Incubated at room temperature for 10 min and 30min on ice. Cells were collected by centrifugation at 7100 xg rpm for 10 min at 4C. The supernatant was discarded and the pellet washed twice with 20mL PBS buffer, pellet was kept at 4C and used immediately for DNA extraction.

value: xylX::pXVENC4 (CJW5796)

tag: expression

value: Venus fluorescent protein at gene locus expressed at xylose locus, induced 1h30min xylose 0.03% before fixation


title: Slx5D_1

value: MHY7919 MATalpha his3delta_1 leu2delta_0 met15delta_0 ura3delta_0 lys2delta_0 slx5delta_::kanMX


title: Mock IP


title: Slx5D_0


title: Slx5D_2


title: Set1D_1


title: Input Swarmer

source_name: Synchonized swarmer population

value: redN::redN-venus (CJW5534)

value: natively expressed at gene locus


title: Set1D_0


title: RedN-Venus IP AsyncRep2


title: RedN-Venus IP AsyncRep1


title: RedN-Venus IP Swarmer


title: Ulp2D_2

value: MHY5033 MATalpha his3delta_1 leu2delta_0 met15delta_0 ura3delta_0 lys2delta_0 ulp2delta_::kanMX


title: Input AsyncRep2


title: SX3024_OrV_2

city: Cambridge

first: Eric

middle: Alexander

last: Miska

address: Tennis Court Road

department: Gurdon Institute

country: United Kingdom

institute: University of Cambridge

data_processing:
Alignments were to the C elegans WS236 coding sequences downloaded from wormbase using bowtie2 (version 2.1.0)
Counts were obtained from alignment files using Bedtools version 2.21.0
Normalized counts were obtained using DESeq running through R version 3.1.1
Genome_build: WS236
Supplementary_files_format_and_content: tab-delimited text files include normalised count numbers for each sample

organism: Caenorhabditis elegans

source_name: whole animal,mixed population

growth_protocol: All strains were grown on NGM plates seeded with HB101 E coli

extract_protocol:
Animals were crack frozen in liquid nitrogen and RNA was extracted with Trisure
Libraries were prepared with the NEBNext Poly(A) mRNA magnetic isolation module followed by the NEBNext Ultra RNA library Prep Kit for Illumina

tag: infection

value: OrV


title: SX3024_OrV_4


title: WT_2

source_name: WT

value: MHY2972 MATa his3delta_1 leu2delta_0 ura3delta_0 lys2delta_0

value: WT


title: WT_1


title: SX3024_OrV_1


title: Input AsyncRep1


title: SX3024_ni_6


title: WT_0


title: SX3024_ni_3


title: SX3024_ni_4


title: SX2914_OrV_2


title: SX2914_OrV_3


title: SX2914_OrV_1


title: SX2914_ni_6


title: N2_OrV_4


title: SX2914_ni_4


title: Ulp2D_1


title: SX2914_ni_5


title: N2_OrV_3


title: 10WK-3

city: hefei

first: xing

middle: yong

last: chen

state: anhui

address: 130 West Changjiang Road

department: School Of Animal Science And Technology

country: China

institute: Anhui Agricultural University

data_processing:
The small RNA sequence reads were pre-processed using FASTXToolkit (fastx_toolkit-0.0.13.2; http://hannonlab.cshl.edu/fastx_toolkit/commandline.html), excluding low-quality reads (ambiguous N, quality < 10 nt, and length <18 nt) as well as 3′ adapter, 5′adapter and poly(A) sequences.
Further annotation analyses were performed using the commercial software CLC Genomic Workbench 5.5.
Briefly, the resulting clean reads were aligned against various databases, including ncRNA, piRNA, and Rfam, allowing a maximum mismatch of 2 nt to remove noncoding RNA, such as rRNA, tRNA, snRNA, and snoRNA.The remaining sequences were analyzed by BLAST Gallus gallus search against Sanger miRBase (version 21.0).
Reads that did not match any database above were marked as non-annotation. Non-annotated sequences were searched against the Gallus gallus genome using the miRCat program included in the sRNAToolkit (http://srna-workbench.cmp.uea.ac.uk/tools/analysis-tools/mircat/). Using default settings, 100 nucleotides of genomic sequence flanking each side of the sequences were extracted for RNA secondary structure prediction using RNAfold [1]. Only with tipical stem-loop hairpins and the free energy lower than -20 kcal/mol could be considered to be potential novel miRNAs.
Normalize the expression of miRNA in eighteen libraries to get the expression of transcript per million (TPM). Normalization formula: Normalized expression = Actual miRNA count/Total count of clean reads×1000000.
Genome_build: Gallus gallus
Supplementary_files_format_and_content: miRNA reads count

organism: Anas platyrhynchos

source_name: 10WK rep3

extract_protocol:
Total RNA was extracted from the tissue using a mirVana miRNA Isolation Kit (Ambion, USA). The purified RNA yield was determined by the absorbance at 260 nm with an ND-2000 NanoDrop spectrophotometer (Thermo Fisher, USA), and RNA quality was evaluated with a BioAnalyzer 2100 system (Agilent Technologies, USA).
RNA smaller than 200 base pairs (bp) was enriched with the mirVana miRNA isolation kit (Ambion). The RNA was then precipitated with ethanol and dissolved in water. Small RNAs had linkers ligated to them and bar-coded cDNAs were prepared using a TruSeq Small RNA Sample Prep Kit (Illumina, USA) following the manufacturer’s instructions.

value: 10 week of age

value: skin

tag: breed

value: Pekin Duck


title: N2_ni_4


title: N2_OrV_1


title: Ulp2D_0


title: Second hit LPS + B

city: Montreal

first: Martin

middle: G

last: Frasch

state: QC

address: 3175, Côte-Sainte-Catherine

department: Dépt. obstétrique-gynécologie

country: Canada

institute: Université de Montréal

data_processing:
Raw data were mapped to the reference genome of the sheep Ovis aris v3.1 from NCBI and Ensembl (GCA_000298735.1) as transcriptome reference.
Index of the reference fasta file were built with Bowtie2, we then trimmed the adaptor of the fastQ files with TrimGalore, and mapped reads to the reference with Tophat2.
The number of reads per gene were counted with HTseq and assembled into a matrix containing the read count of each gene per sample.
Genome_build: GCA_000298735
Supplementary_files_format_and_content: Tab delimited file included read count for each sample

organism: Ovis aries

source_name: microglia

extract_protocol:
Total RNA was extracted from cultured microglia using TRIzol Reagent.
RNAseq libraries were prepared by using Illumina TruSeq RNA Sample Preparation v2 kit (Illumina) and quality control was performed on the BioAnalyzer.

tag: drug

value: Bungarotoxin

tag: lps exposure

value: second hit


title: LPS+B2

value: single hit


title: N2_ni_2


title: SX2966_chip_rep2

data_processing:
Reads were aligned using BWA v. 0.7.7
SAMtools v. 0.1.19 ‘view’ utility was used to convert the alignments to BAM format
Normalized ChIP-seq coverage tracks were generated using the R implementation of BEADS algorithm
Initial ChIP-seq peaks were called using MACS v. 2.1.1. To generate combined peak calls, we used the modified IDR procedure with an IDR threshold of 0.05 to combine replicates
Genome_build: WS220/ce10 assembly of the C. elegans genome
Supplementary_files_format_and_content: narrowpeak file was generated using a modified IDR procedure from the encode project with an IDR threshold of 0.05 to combine replicates
Supplementary_files_format_and_content: wig files were generated using the R implementation of BEADS algorithm and contain normalised Chip-seq coverage tracks.

extract_protocol:
After fixation with formaldehyde, C elegans lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody.
Libraries were prepared according to a modifed Illumina protocol (Ethan Ford's protocol). Briefly, DNA was end-repaired using the NEB ENd repair enzyme Mix. The blunt, phosphorylated ends were treated with Klenow fragment (exo minus, NEB) and dATP to yield a protruding 3- 'A' base for ligation of Illumina's adapters which have a single 'T' base overhang at the 3’ end. After adapter ligation DNA was PCR amplified with Illumina primers  and library fragments of ~250 bp (insert plus adaptor and PCR primer sequences) were size selected with AMPure XP beads.

tag: antibody

value: Abcam ab290


title: LPS+B3


title: N2_ni_1


title: LPS+B1


title: LPS+A3


title: 4WK-1

source_name: 4WK rep1

value: 4 week of age


title: SX2966_input_rep1


title: 4WK-2

source_name: 4WK rep2


title: SX2966_input_rep2


title: LPS+A2


title: SX2966_chip_rep1


title: LPS+A1


title: 1DB-3

source_name: 1DB rep3

value: 1 day of age


title: 1DB-2

source_name: 1DB rep2


title: 1DB-1

source_name: 1DB rep1


title: 15EM-1

source_name: 15EM rep1

value: 15 embryonic day


title: 11EM-3

source_name: 11EM rep3

value: 11 embryonic day


title: 10WK-1

source_name: 10WK rep1


title: Fruiting bodies rep1

city: Beijing

first: junjie

last: shao

state: --- Select a state ---

address: Rm 409, Ke-Yan-Lou, No. 151 Malianwa North Road, Haidian District, Beijing 100093, P.R.China

institute: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China

data_processing:
Illumina Casava1.9 software used for basecalling
Adapters sequences were trimmed and low-quality reads containing at least ten percent of Ns were removed from Sequenced reads, and then mapped to Ganoderma lucidum whole genome using TopHat v2.1.0 with default parameters.
Then the transcripts were reconstructed through Cufflinks using default parameters. The number of reads in the exons of the reconstructed transcripts were counted and normalizd by the size of the reconstructed transcripts and by the size of the library.
The mapping of RNA-seq reads to the reference genome was visualized using Tablet (v1.12.12.05)
Genome_build: GanLuc1.0
Supplementary_files_format_and_content: tab-delimited text files include include FPKM values

organism: Ganoderma lucidum G.260125-1

source_name: Fruiting bodies

growth_protocol: The mycelia were cultivated in potato dextrose agar (PDA) medium in the dark at 28 °C for 7 days. The primordia and fruiting bodies were cultivated on Quercus variabilis logs.

extract_protocol:
RNA was harvested using plant total RNA extraction kit (Tiangen, China). Genomic DNA contaminations were removed using RNase-free DNase I (Tiangen, China).
The strand-specific RNA-library was constructed following the SMART-seq protocol, then sequenced following standard Illumina protocols.

value: Fruiting bodies

value: wild type


title: Mycelia rep1

source_name: Mycelia

value: Mycelia


title: 15EM-3

source_name: 15EM rep3


title: 11EM-2

source_name: 11EM rep2


title: 15EM-2

source_name: 15EM rep2


title: Primordia rep1

source_name: Primordia

value: Primordia


title: Exp 1_control

status: Public on Aug 10 2017

city: Pushchino

first: Olga

middle: N

last: Ozoline

state: Moscow Region

address: Institutskaya

department: Functional Genomics and Cellular Stress

country: Russia

institute: Institute of Cell Biophysics of Russian Academy of Sciences

description:
Control sample
Experiment_1_CLC_GW_peaks.txt
Experiment_1_Matcher_peaks.txt

data_processing:
Raw data were quality filtered on the Galaxy server (Filter by Quality tool, Q≥20) providing 99% probability of correct sequencing for all nucleotides in each read. As a result, the sets contain 31,656,551 and 45,396,252 sequences in the control and immunoprecipitated samples of the first experiment, and, respectively, 7,493,528 and 8,214,737 reads for the second experiment.
Two different approaches were used to align sequence reads to the genome of E. coli K-12 MG1655 (U00096.3). First, quality controlled reads were mapped using the CLC Genomics Workbench version 7.5.1 (CLC GW, Bio-Qiagen, Aarhus, Denmark) with either default settings for the length and similarity fractions (0.5 and 0.8, respectively, see supplemental Table 2 of the paper) or with the most stringent criteria (1.0 and 1.0, respectively, deposited). Reads with sequences aligned to multiple genomic regions were ignored.
To apply the second approach variable in length reads from Illumina MiSeq were first trimmed from both sides to obtain a set of standard 50 nt sequences taken from the middle of longer reads. Reads shorter than 50 nt were discarded. Then, all four sets from both experiments were aligned to the genome using the Matcher program (available at: http://www.mathcell.ru/DnaRnaTools/Matcher.zip). This software maps only 5’-ends if the reads correspond to the top strand of genomic DNA, or only 3’-ends, if they are aligned to the bottom strand. As such, the signals from fully complementary reads will match the same (left) position. Reporting the distribution of matching reads across the genome the program also evaluates reads with multiple occurrence and their positions.
Only ideal correspondence of sequence reads to the genome in both cases was permitted. The profiles obtained for experimental and control samples were normalized by the scaling method initially offered by Affymetrix for microarray data analysis and later implemented in several other approaches. This method assumes unaffected protein occupancy at most genomic positions and quantifies the scaling factor on the basis of corrected mean values obtained after removal of 2% signals with highest and lowest intensities from both control and experimental sets.
After normalization, the read counts were estimated in the running windows of 35 bp, and the ratios R between values obtained for experimental and control libraries were calculated. Peaks were localized requiring R≥1.5 for at least 50% positions of at least 60 bp genomic region in length.
Genome_build: E. coli K-12 MG1655 (U00096.3)
Supplementary_files_format_and_content: Processed data include read pileups obtained in two ChIP-seq experiments by two different approaches (files 1-4), the set of regions bound by Dps in both experiments (file 5) and the set of regions unbound by Dps in both experiments (file 6). All processed data are provided as tab-delimited .txt files.

organism: Escherichia coli str. K-12 substr. MG1655

source_name: Bacterial cells

growth_protocol: Bacterial cells were grown aerobically in M9 medium supplemented with 0.2% glucose and 5% LB at 37°C under constant shaking (~120 rpm) in a water bath and harvested at OD ~0.6.

extract_protocol:
For immunoprecipitation, 800 μl of collected chromatin (~1000 μg of protein) were incubated on a rotating wheel over night at 4°C with 10 μg of either rabbit anti-Dps antibodies (experimental sample) or rabbit pre-immune IgG (negative control). Next day 30 μl of Ultra Link Protein A/G beads (Thermo Scientific, USA) were added to the samples, and incubation was allowed for a further 2 hours at 4°C on a rotating wheel. The beads were washed 3 times with 1 ml of the low-salt buffer (50 mM HEPES pH 7.5, 140 mM NaCl, 1% Triton X-100, 1xPIC) and then with 1ml of the high-salt buffer (500 mM NaCl). Immunoprecipitated DNA-protein complexes were removed from the beads by 3 h shaking at 65°C and 1000 rpm in 110 μl of freshly prepared elution buffer, containing 100 mM NaHCO3 and 1% SDS. After centrifugation at 3000 rpm for 5 minutes, 100 μl of the supernatant was transferred to a new tube, and the DNA was purified with a PCR Purification Kit (Qiagen, Germany). The DNA concentration was measured on Qubit 2.0 using Qubit dsDNA HS Assay kit. A total of 8-10 identical samples were combined and concentrated to a volume of 30-50 μl containing 5-10 ng of the DNA.
ChIP-seq libraries were prepared from 5-10 ng of the DNA samples with the NebNext® Ultra™ DNA Library Prep Kit for Illumina (New England Biolabs, MA, USA) following the manufacturer’s instructions. For the final amplification of the library 15 PCR cycles were used. Size distribution and concentration of the amplicons was checked on the Bioanalyzer 2100 (Agilent, USA). In the first experiment, the maximum was at about 300 bp, and ChIP libraries were sequenced using 50 nt single-end read protocol on the Illumina HiSeq system (Illumina, USA) of the Genomics Facility in the Centre for Genomic Regulation (Barcelona). In the second experiment, the maximum was at about 450 bp, and samples were sequenced using standard paired-end 2*150 nt protocol on the MiSeq system (Illumina, USA) in the Immanuel Kant Baltic Federal University (Kaliningrad).

treatment_protocol: ChIP-seq experiments were performed in duplicate using similar growth condition and protocols for chromatin immunoprecipitation. The first experiment was done in the Centre for Genomic Regulation (CRG, Barcelona, Spain), while the second one in the Immanuel Kant Baltic Federal University (Kaliningrad, Russia). In both cases, bacterial cells were grown aerobically at 37°C until OD600~0.6 were treated with formaldehyde (final concentration of 1%, 20 min incubation). Cross-linking was stopped with glycine (final concentration of 450 mM). After 5 minutes of incubation the cells were pelleted by centrifugation at 14,000 rpm for 15 minutes (+4°C), washed twice with 5 ml of PBS and resuspended in 1.3 ml of ice-cold immunoprecipitation buffer prepared from 50 ml of buffer containing 100 mM NaCl, 50 mM Tris-HCl (pH 8.1), 5 mM EDTA, 0.2% NaN3, 0.5% SDS, and 25 ml of buffer containing 100 mM Tris-HCl (pH 8.6); 100 mM NaCl; 5 mM EDTA; 0,2% NaN3, 5% Triton-X-100. Then phenylmethylsulfonyl fluoride (final concentration of 1 mM) or 20 μl of Protease Inhibitor Cocktail (PIC, Sigma) for the first and the second experiment, respectively, were added followed by incubation for 30 min at +4°C.

tag: growth stage

value: log-phase

tag: medium

value: rabbit pre-immune IgG

tag: ngs platform

value: Illumina HiSeq

tag: sequencing mode

value: 50 nt single-end read protocol


title: Exp 1_IP sample

description:
Experimental sample
Experiment_1_CLC_GW_peaks.txt
Experiment_1_Matcher_peaks.txt

value: M9 medium supplemented with 0.2% glucose and 5% LB

value: Anti-Dps antibodies


title: hPBMC_input

city: Yokohama

first: Takahiro

last: Suzuki

state: Kanagawa

address: 1-7-22 Suehiro-cho, Tsurumi-ku

department: Center for Integrative Medical Sciences

country: Japan

institute: RIKEN

data_processing:
The ChIP-seq FASTQ data was mapped to human genome hg19 with bowtie2.
Peak-calling was done by MACS2 with p-value cut-off 10E-5.
Genome_build: hg19
Supplementary_files_format_and_content: bed files were generated by MACS2 with cut-off p-value 10E-5.

source_name: hPBMC

growth_protocol: human peripheral blod mononuclear cells were acquired from Lonza (#CC-2702).

extract_protocol:
Lysates were clarified from sonicated nuclei and RUNX1 or TET2-DNA complexes were isolated with antibody.
ChIPed DNA was subjected to the library construction with KAPA Library Preparation Kit (KAPA Biosystems, Imc.,MA, USA) according to the manufacture’s instruction.

value: Male

tag: tissue

value: Blood

tag: cell type

value: PBMC

tag: chip antibody

value: none (input)


title: hPBMC_TET2-ChIP-seq

value: anti-human TET2 antibody (diagenode,cat no. c15410255)


title: Ctrl_3

city: Kunming

first: Jiandong

last: Shi

state: Yunnan

address: 935# Jiaoling Road

department: Department of Vaccine Research

institute: Institute of Medical Biology

description: small RNA

data_processing:
Briefly, the sequences were blasted to human reference genome hg38 (GRCh38.p7), the RFam and Repbase to identify possible mRNA, rRNA, tRNA, snRNA, snoRNA and other ncRNAs.
To identify the known miRNAs, the remaining sequences were aligned to the miRBase (release 21.0) by using Bowtie. Matched sequences with no more than one mismatch were considered as known miRNAs.
Besides, the unmatched sequences were used to predict candidate novel miRNAs using miRDeep2. The hairpin RNA structures containing the unmatched sequences were predicated complying with criteria of the pre-miRNAs to identify potentially novel miRNAs.
Expression level of miRNAs was determined by normalizing reads to tags per million (TPM) counts. The TPM was calculated as follows: normalized expression, TPM = (actual miRNA count/number of total clean read) × 106.
Genome_build: human reference genome hg38 (GRCh38.p7)
Supplementary_files_format_and_content: sequence, count and TPM expression value of novel and known miRNAs

source_name: human fibroblast KMB17 cells

growth_protocol: Human fibroblast KMB17 strain (Institute of Medical Biology, CAMS), a normal diploid cell line that was originated from fetal lung tissue, was cultured in Minimum Essential Medium (MEM) supplemented with 10% bovine serum (Minhai Biotech, Beijing, China) at 37°C in 5% CO2.

extract_protocol:
At 48 h postinfection, total RNA from HSV-1 infected and uninfected KMB17 cells was isolated with Trizol regeant (Invitrogen) followed by digestion with DNase I (Epicentre) for 15 min at 37°C.
RNA libraries were prepared for sequencing using standard Illumina protocols

treatment_protocol: KMB17 cells were mock- infected or infected with HSV-1 at a multiplicity of infection (m.o.i) of 1.

tag: cell line

value: Human fetal lung tissue-derived fibroblast cells

tag: infected with

value: nothing (mock)

tag: passages

tag: virus strain


title: hPBMC_RUNX1_ChIP-seq

tag: gender

value: anti-human RUNX1 antibody (Abcam, cat.no ab23980)


title: hPC_1.2KD HypER

city: Frankfurt am Main

first: Christoph

middle: Michael

last: Zehendner

address: Theodor Stern Kai 7

institute: Goethe University

description:
total RNA ribosomal depleted
HypERrlnc knockdown with LNA GapmeR
human PC HypERrlnc KD_FPKM.txt
hPC_2KD

data_processing:
(RNA-SEQ) Reads were mapped with Tophat default parameters to MM10 (mouse brain primary pericytes), or GRCH38 (human primary pericytes).  Afterwards Cufflinks was utilized with the default attributes for gene quantification.
Genome_build: MM10 (mouse brain primary pericytes), or GRCH38 (human primary pericytes)
Supplementary_files_format_and_content: Tab delimited text file which includes FPKM values for each sample

source_name: human primary pericytes

growth_protocol: Human pericytes (passages 2-9; from ScienCell, Carlsbad, CA, USA) were cultured as recommended by the manufacturer. Cells were kept at 5% CO2, 20% O2, 37°C and humidified atmosphere. Pericyte medium consisted of DMEM Glutamax (Gibco, Life Technologies, Carlsbad, CA, USA) supplemented with Penicillin/Streptomycin (Roche Diagnostics) and 10% fetal calf serum. Primary mouse brain pericytes were isolated and cultured as documented in detail (Tigges et al. Microvascular Research 84 (2012) 74–80).

extract_protocol:
RNA deep sequencing was performed by analyzing ribosomal depleted total RNA from human pericytes. RNA was isolated using a RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions including DNA digestion.
RNA was fragmented and primed for cDNA synthesis. Human samples: Libraries were created using a NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina as recommended by the manufacturer. Mouse sample libraries were created using a Scriptseq v2 Kit from Illumina SSV21124 as recommended by the manufacturer.

treatment_protocol: Hypoxia was induced using a hypoxic incubator (Labotect, Göttingen, Germany). Cell culture medium was pre-equilibrated overnight at 1% O2, 5% CO2 in a humidified atmosphere. Normoxic cell culture medium was carefully switched to hypoxic medium and hypoxic pO2 levels were verified by measuring VEGFA induction by qPCR and pO2 levels with a hypoxia sensing probe from Oxford Optronix (Oxford, UK) as described in detail before (Zehendner et al. Plos One 2013). Cells were transfected after reaching a confluency of 60-80% using Opti-MEM Medium, Lipofectamine RNAiMAX (both from Life technologies, Carlsbad, CA, USA) and 50 nmol/l of LNA GapmeR (Exiqon, Vedbaek, Denmark). After 4h transfection medium was exchanged to the appropriate cell culture medium. 48h upon transfection RNA was isolated and processed for RNA Sequencing.

value: human primary pericytes

tag: passage

value: passage 2-9

tag: treatment

value: HypERrlnc knockdown LNA GapmeR


title: hPC_1.3KD HypER

description:
total RNA ribosomal depleted
HypERrlnc knockdown with LNA GapmeR
human PC HypERrlnc KD_FPKM.txt
hPC_3KD


title: 293T-RUNX1_input

growth_protocol: Jurkat was acquired from the BRC and were cultured in RPMI-1640 medium (Wako Pure Chemical Industries), supplemented with 10% fetal bovine serum and penicillin/streptomycin (100 U/mL, 100 µg/mL; Thermo Fisher Scientific Inc).

treatment_protocol: For the RUNX1 knocking-down, shRNA expression lentivirus vectors were prepared from pLKO.1 shRUNX1 puro (#45816) and pLKO.1 puro (#8453) empty plasmids. The shRNA lentivirus vectors were transduced at 10 MOI and the transduced cells were selected 2ug/ml puromycine for 1 week.

value: Kidney


title: Jurkat_NCKD TET2-ChIP-seq

description: Negative control sh RNA transduced 293T cells

source_name: Jurkat


title: Jurkat RUNX1KD TET2-ChIP-seq

description: RUNX1 knocking-down 293T cells


title: hPC_0.3ctrl

description:
total RNA ribosomal depleted
Scramble Control
human PC HypERrlnc KD_FPKM.txt
hPC_3ctr

value: Scramble Control GapmeR


title: hPC_0.1ctrl

description:
total RNA ribosomal depleted
Scramble Control
human PC HypERrlnc KD_FPKM.txt
hPC_1ctr


title: hPC_1.1KD HypER

description:
total RNA ribosomal depleted
HypERrlnc knockdown with LNA GapmeR
human PC HypERrlnc KD_FPKM.txt
hPC_1KD


title: 293T-RUNX1_RUNX1_ChIP-seq


title: hPC_0.2ctrl

description:
total RNA ribosomal depleted
Scramble Control
human PC HypERrlnc KD_FPKM.txt
hPC_2ctr


title: RUNX1KD Input

description: RUNX1 knocking-down cells

data_processing:
The ChIP-seq FASTQ data was mapped to human genome hg19 with bowtie2.
Peak-calling was done by MACS2 with p-value cut-off 10E-10.
Genome_build: hg19
Supplementary_files_format_and_content: bed files were generated by MACS2 with cut-off p-value 10E-10

source_name: Blood

value: Jurkat


title: RUNX1_ChIP-seq


title: TET2-ChIP-seq


title: Ctrl_2

description: small RNA


title: 20EM-1

source_name: 20EM rep1

tag: developmental stage

value: 20 embryonic day

value: skin

tag: breed

value: Pekin Duck


title: 4WK-3

source_name: 4WK rep3

value: 4 week of age


title: ram1-2 root control replicate 3

city: Castanet-Tolosan

first: Christophe

last: Roux

address: Chemin de Borde Rouge

country: France

institute: University of Toulouse/CNRS

description:
568_NM_3
processed data file:
568M vs. 568NM.xls

data_processing:
Reads trimming and filtration: Short reads generated on Illumina platforms (150bp bp from Hiseq2000) were trimmed based on the quality scores (limit: 0.05), end ambiguity (maximum number of ambiguities: 2) and adaptor sequences using the commercial software CLC Genomic Workbench (v9, CLC Bio, Denmark). The reads less than 50bp after trimming were discarded.
Read mapping: trimmed reads from all triplicates of Petunia samples, were mapped back to the P axilaris genre repertoire (32'928 genes in P. axillaris v4 genome available at https://solgenomics.net ). To calculate gene expression, RNA-seq functionality in CLC Genomic Workbench software was used, with the settings of minimum mapped read length fraction at 0.9 and minimum similarity at 0.9. To reveal Petunia genes significantly differentially regulatedduring mycorrhizal symbiosis,  RPKM of Petunia wild type (W115) and ram1 (568) from mycorrhizal samples (M) were compared to non-mycorryzal samples (NM) using proportion-based test statistics with a False Discovery Rate (FDR) correction for multiple testing. Petunia Genes were considered as significantly upregulated when meeting the requirements of RPKM fold change>5 and FDR corrected p value<0.05.
Genome_build: P. axillaris v4 genome available at https://solgenomics.net

organism: Petunia x hybrida

source_name: ram1-2 root control

growth_protocol: Petunia plants W115 and ram1-2 (Rich et. al Plant Physiol 2015) where surface sterilized with sodium hypochlorite (1,3%, 10 min) and germinated on soil. Four weeks old plantlets were transfered in 10x10cm pots containing a 75% sand, 25% unfertilized soil mixture. Plants were grown in growth chambers with a day/night cycle of 16h (25°C)/8h (20°C) and fertilized weekly with a solution containing 3 mM MgSO4, 0.75 mM KNO3, 0.87 mM KCl, 0.2 mM KH2PO4, 1.52 mM Ca(NO3)2, 0.02 mM NaFeEDTA, 11 µM MnSO4, 1 µM ZnSO4, 30 µM H3BO3, 0.96 µM CuSO4, 0.03 µM (NH4)6Mo7O24, and 0.01 µM Na2MoO4. Samples were harvested 15 days post inoculation.

extract_protocol:
Samples of about 100mg were frozen in liquid nitrogen, stored at -80°C and lyophilised before extraction. Total RNA was extracted from lyophilized roots using the guanidinium thiocyanate-phenol-chloroform method and DNAse treated on column (DNA, RNA and protein purification, Macherey-Nagel, Switzerland)
Preparation of 12 libraries and 2 x 100bp pair-end Illumina HiSeq mRNA sequencing (RNA-Seq) was performed by Beckman Coulter Genomics (Danvers, MA, USA)

treatment_protocol: Plants were inoculated with around 30g of soil inoculum of Rhizophagus irregularis (MUCL 43204)

tag: genotype/variation

value: root


title: Input

value: Male


title: RHBDF1+P_CAPseq

city: oxford

first: Jim

last: Hughes

address: Weatherall Institute Of Molecular Me

department: MHU

institute: University of Oxford

description: Contains human sequence chr16: 47,861 - 63,210; hg18

data_processing:
Data was aligned with STAR align (default parameters) to the human hg19 genome to map transcription initiation sites within the human gene sequence of the RHBDF1 with (chr16: 47,861 - 63,210; hg18) and without its promoter (chr16: 47,911 - 60,819; hg18), that was targeted into the RMCE system within the mouse α-globin locus (Lynch et al., 2012, DOI: 10.1038/emboj.2011.399).
downsampling bam files to smallest sample, using all mapped, properly paired reads as the reference count
generating raw coverage bigwig of the above counts
Genome_build: hg19
Supplementary_files_format_and_content: downsampled coverage bigwig

organism:
Homo sapiens
Mus musculus

source_name: mES derived embryoid bodies day 7

growth_protocol: ES-cell derived EB were obtained by culturing ES cells in the absence of LIF and the presence of transferrin and ascorbic acid as described in Tufarelli et al., 2003 (PMID  12730694)

extract_protocol:
Total RNA was extracted using TRI Reagent (Sigma-Aldrich, T9424) and then fragmented using the NEBNext Magnesium RNA Fragmentation Module.
Fragmented RNA was cleaned up using RiboMinus Concentration Module (Life Technologies) and the 3’ adapters were ligated using a modified version of the NEBNext Small RNA Library Prep Set for Illumina (Multiplex Compatible). Samples were subjected to a second round of clean up using RiboMinus Concentration Module and then subsequently treated with Calf Intestinal Phosphatase (CIP) and Tobacco Acid Phosphatase (TAP). The reaction was cleaned up using the RiboMinus Concentration Module (Life Technologies) as before, and annealing of the SR RT (reverse transcription) primer and ligation of the 5’ adapter was performed. Reverse transcription was carried out using the Superscript III First Strand Synthesis system, followed by library amplification and cleaned up using a QIAquick PCR Purification kit (Qiagen, 28104).

tag: strain

value: ES derived embryoid bodies

tag: stage of differentiation

value: day 7


title: RHBDF1-P_CAPseq

description: Contains human sequence chr16: 47,911 - 60,819; hg18


title: ram1-2 root +R.i replicate 1

description:
568_M_1
processed data file:
568M vs. 568NM.xls

source_name: ram1-2 root +R.i

tag: inoculated with

value: Rhizophagus irregularis MUCL 43204


title: ram1-2 root +R.i replicate 2

description:
568_M_2
processed data file:
568M vs. 568NM.xls


title: WT root control replicate 3

description:
W115_NM_3
processed data file:
w115-M vs. w115-NM.xls

source_name: WT root control


title: 11EM-1

source_name: 11EM rep1

value: 11 embryonic day


title: ram1-2 root control replicate 2

description:
568_NM_2
processed data file:
568M vs. 568NM.xls


title: ram1-2 root control replicate 1

description:
568_NM_1
processed data file:
568M vs. 568NM.xls


title: WT root control replicate 1

description:
W115_NM_1
processed data file:
w115-M vs. w115-NM.xls


title: WT root control replicate 2

description:
W115_NM_2
processed data file:
w115-M vs. w115-NM.xls


title: WT root +R.i replicate 2

description:
W115_M_2
processed data file:
w115-M vs. w115-NM.xls

source_name: WT root +R.i


title: CD4-ICOSpos-B450

city: Houston

first: Spencer

last: Wei

address: 7455 Fannin St.

department: Immunology

institute: MD Anderson Cancer Center

description: anti-PD-1 rep2

data_processing:
RNAseq data was aligned to GRCm38 using TopHat2 with parameter --library-type=fr-firststrand
Gene read counts were generated using htseq-count
Bioconductor R package DESeq2 was used to normalize and analyze the RNA read count matrix
Genome_build: GRCm38
Supplementary_files_format_and_content: comma seperated csv files include read counts matrix for all the samples

source_name: tumor infiltrating ICOS+ CD4 T cells

extract_protocol:
Activated tumor infiltrating ICOS+ CD4 T cells were sorted directly into RLT buffer (volume) using a FACS AriaFusion cell sorter (BD).  Lysates were homogenized using a QiaShredder and RNA extracted using RNeasy Micro kit (Qiagen) according to the manufacturer’s protocol.
Due to low RNA yield from sorted T cell populations from individual mice, libraries were prepared using Ovation single-cell RNAseq system (NuGEN).  Indexed libraries were analyzed by 76-nucleotide paired end sequencing on a HiSeq 4000 system (Illumina).

value: tumor infiltrating ICOS+ CD4 T cells

tag: age

value: post MC38 tumor cell injection day 12


title: CD4-ICOSpos-B451

description: anti-PD-1 rep3

tag: tissue


title: CD4-ICOSpos-B447

description: anti-PD-1 rep1


title: CD4-ICOSpos-B442

description: anti-CTLA-4 rep2


title: CD4-ICOSpos-B444

description: anti-CTLA-4 rep3


title: CD4-ICOSpos-B435

description: Control rep3


title: CD4-ICOSpos-B440

description: anti-CTLA-4 rep1


title: CD4-ICOSpos-B430

description: Control rep1


title: CD4-ICOSpos-B431

description: Control rep2


title: ram1-2 root +R.i replicate 3

description:
568_M_3
processed data file:
568M vs. 568NM.xls


title: WT root +R.i replicate 1

description:
W115_M_1
processed data file:
w115-M vs. w115-NM.xls


title: WT root +R.i replicate 3

description:
W115_M_3
processed data file:
w115-M vs. w115-NM.xls


title: nuclei_588

city: La Jolla

first: Joseph

middle: R

last: Ecker

state: CA

address: 10010 North Torrey Pines Road

department: Genomic Analysis Laboratory

institute: HHMI-Salk-Institute

data_processing:
genome build: mm10
For multiplexed single nucleus methylome samples, reads were first trimmed to remove sequencing adaptors using Cutadapt 1.11 with the following parameters in paired ends mode: -f fastq -q 20 -m 62 -a AGATCGGAAGAGCACACGTCTGAAC -A AGATCGGAAGAGCGTCGTGTAGGGA. For singleplex samples, -m parameter was set to 40. For multiplexed samples, 16bp was further trimmed from both 5’- and 3’- ends of R1 and R2 reads to remove random primer index sequence and C/T tail introduced by Adaptase, with the following parameters: -f fastq -u 16 -u -16 -m 30. For singleplex samples, 10bp was trimmed from both 5’- and 3’- ends of R1 and R2 reads with the following parameters: -f fastq -u 10 -u -10 -m 30. Trimmed reads for mouse and human single nuclei were mapped to mm10 and hg19 reference genome, respectively. R1 and R2 reads were mapped separately as single-ended reads using Bismark v0.15.0 with parameter --bowtie2. --pbat option was activated for mapping R1 reads. Resulted bam files were sorted using samtools 1.3 sort, followed by removal of duplicate reads using Picard 1.141 MarkDuplicates with the option REMOVE_DUPLICATES=true. Non-clonal reads were further filtered for minimal MAPQ = 30 using samtools view with option -q30. To prevent regional mCH level estimation from being skewed by rare reads failed to be bisulfite converted, reads with read-level mCH level greater than 0.7 were excluded. The calling of unmethylated and methylated base calls were performed by call_methylated_sites of Methylpy (https://github.com/yupenghe/methylpy/) (1–3).
Supplementary_files_format_and_content: tab delimited text files of methylcytosine calls; columns in allc files are: column 1 - chromosome; column 2 - position; column 3 - strand; column 4 - class; column 5 - mC reads; column 6 - total reads; column 7 - methylated (Boolean value indicating the result of statistical test for methylated cytosines)

source_name: Mouse frontal cortex

growth_protocol: For the production of single neuron methylomes from layer dissected mouse frontal cortex tissue, eight week old C57BL/6J male mice were purchased from Jackson Laboratories, Bar Harbor ME, and allowed a week of acclimation in our animal facility with 12 h light/dark cycles and food ad libitum, before dissections. The human brain specimen was obtained from the NICHD Brain and Tissue Bank for Developmental disorders at the University of Maryland, Baltimore, MD. The frozen middle frontal gyrus tissue belonged to a 25-year-old Caucasian male (UMB#4540) with a PMI of 23 h.

extract_protocol:
A detailed protocol for MethylC-seq libraries preparation can be found in Urich et al., (2015). MethylC-seq library preparation for base-resolution whole-genome bisulfite sequencing. Nat Protoc 10, 475-483.
Nuclei isolation from mouse and human cortical tissues was performed as described in (Lister et al., 2013) with the following modifications: Proteinase inhibitor (11836153001, Roche) and RNAse Inhibitor (30 U/ml, PRN2611 from Promega) was added to the lysis buffer and sucrose gradients. After centrifugation, nuclei were resupended in 0.5% BSA (AM2616, Ambion) PBS (Ca2+ and Mg2+ free, 14190-144 from Life Technologies,) with protein and RNAse inhibitors. Isolated nuclei from mouse and human tissues were labeled by incubation with 1:1000 dilution of AlexaFluor488 conjugated anti-NeuN antibody (MAB377X, Millipore) at 4°C for 1 hour. Nuclei isolated from CLSun1-G35-Cre line were incubated with AlexaFluor647 conjugated anti-NeuN antibody (anti-NeuN antibody MAB377 labeled using Apex Alexa Fluor 647. A10475, Life Technologies) and AlexaFluor488 conjugated anti-GFP antibody (A21311, Life Technologies). Fluorescence activated sorting of single nuclei was performed using a BD Influx sorter with an 85 µm nozzle at 22.5 PSI sheath pressure. Single nuclei were sorted into each well of a 384-well plate preloaded with 2 µl of Proteinase K digestion buffer (1 µl M-Digestion Buffer, 0.1 µl 20 µg/µl Proteinase K and 0.9 µl H2O). The alignment of receiving 384-well plate was performed by sorting sheath flow into wells of an empty plate and making adjustment based on the liquid drop position. Single cell (1 drop single) mode was selected to ensure the stringency of sorting.
Steps of library preparation prior to SPRI purification were performed in a horizontal laminar flow hood to minimize environmental DNA contamination. Bisulfite conversion of single nuclei was carried out using Zymo EZ-96 DNA Methylation-DirectTM Kit (Deep Well Format, cat. #D5023) following the product’s manual with reduced reaction volume. 384-well plate (ThermoFisher Armadillo PCR Plate cat. # AB2384) containing FACS isolated single nuclei were heated at 50°C for 20 min. 25 µl CT Conversion Reagent was added to each well followed by pipetting up-and-down to mix. Plates were treated with a following program using a thermocycler: 98°C for 8 min, 64°C for 3.5 hours and 4°C. Each well of Zymo-Spin™ I-96 Binding Plates was preloaded with 150 µl M-binding buffer. Bisulfite conversion reactions were transferred from 384-well plates to I-96 Binding Plates followed by pipetting up-and-down to mix. I-96 Binding Plates were centrifuged at 5,000g for 5 min. Wells were washed with 400 µl of M-Wash Buffer followed by centrifugation at 5,000g for 5 min. 200 µl of M-Desulphonation Buffer was added to each well and was incubated for 15 min at room temperature before removed by centrifugation at 5,000g for 5 min. Each well was then washed with 400 µl of M-Wash Buffer for two rounds. 12 µl of M-Elution Buffer was added to each well and was incubated for 5 min at room temperature. I-96 Binding Plate was placed above a 96-well PCR plate (Applied Biosystems MicroAmp® EnduraPlateTM cat. # 4483348) and was centrifuged at 5,000g for 3min. 9 µl of eluted DNA was commonly collected in each well of the PCR plate. 1 µl of 5 µM P5L-AD002-N9, P5L-AD006-N9, P5L-AD008-N9 or P5L-AD010-N9 indexed random primer was added to each well of 96-well plate followed by mixing with vortexing. All DNA oligos were purchased from Integrated DNA Technologies (IDT). Plate was heated at 95°C for 3 min to denature the sample and was immediately chilled on ice for more than 2 min. 10 µl enzyme mix containing 2 µl of Blue Buffer (Enzymatics cat. # B0110), 1 µl of  10mM dNTP (NEB cat. # N0447L), 1 µl of Klenow exo- (50U/µl, Enzymatics cat. # P7010-HC-L) and 6 µl H2O. After mixing with vortexing, plate was treated with a following program: 4°C for 5 min, ramp up to 25°C at 0.1°C/sec, 25°C for 5 min, ramp up to 37°C at 0.1°C/sec, 37°C for 60 min, 4°C. 2 µl of Exonuclease 1 (20U/µl, Enzymatics cat. # X8010L) was added to each well followed by mixing with vortexing. Plate was incubated at 37°C for 30 min and then 4°C. 17.6 µl of home-made SPRI beads was added to each well. Sample/bead mixture from four plates indexed using distinct indexed random primers were combined followed by pipetting up-and-down to mix. Sample/bead mixture was incubated at room temperature for 5 min before placed on a 96-well magnetic separator. Supernatant was removed from each well followed by three rounds of washing with 180 µl of 80% ethanol. After air drying beads at room temperature, 10 µl M-Elution buffer was added to each well to fully resuspend the beads. Eluted sample was transferred to a new 96-well PCR plate. PCR plate was heated at 95°C for 3 min to denature the sample and was immediately chilled on ice for more than 2 min. 10.5 µl Adaptase (Swift Biosciences) mix (2 µl Buffer G1, 2 µl Reagent G2, 1.25 µl Reagent G3, 0.5 µl Enzyme G5, 0.5 µl Enzyme G6 and 4.25 µl M-Elution buffer) was added into each well followed by mixing with vortexing. Plates was incubated at 37°C at 30 min and then 4°C. 30 µl PCR mix (25 µl KAPA HiFi HotStart ReadyMix, KAPA BIOSYSTEMS, cat. # KK2602, 1 µl 30 µM P5 indexing primer and 5 µl 10 µM P7 indexing primer) was added into each well followed by mixing with vortexing. PCR plate was treated with the following program: 95 °C for 2 min, 98°C for 30 sec, 17 cycles of (98°C for 15 sec, 64°C for 30 sec, 72°C for 2min), 72°C for 5 min then 4°C. PCR products were cleaned up using 0.8x SPRI beads and was combined into one tube for each 96–well plate. Pooled PCR product was resolved on 2% agarose gel, smear between 400 bp and 2 Kb were excised and purified using QIAquick Gel Extraction Kit (Qiagen cat. # 28706). Library concentration was determined using Qubit® dsDNA HS (High Sensitivity) Assay Kit (Invitrogen cat. # Q32851).

value: frontal cortex

tag: Sex

value: 8 wk


title: nuclei_589


title: nuclei_598


title: nuclei_591


title: nuclei_594


title: nuclei_590


title: nuclei_599


title: nuclei_586


title: nuclei_584


title: nuclei_600


title: nuclei_595


title: nuclei_587


title: nuclei_582


title: nuclei_579


title: nuclei_581


title: nuclei_578


title: nuclei_597


title: nuclei_577


title: nuclei_592


title: nuclei_575


title: nuclei_576


title: nuclei_573


title: nuclei_574


title: nuclei_583


title: nuclei_572


title: nuclei_571


title: nuclei_570


title: nuclei_596


title: nuclei_568


title: nuclei_569


title: Ctrl_1

description: small RNA

tag: cell type

value: Human fetal lung tissue-derived fibroblast cells

value: nothing (mock)


title: nuclei_567


title: nuclei_566


title: nuclei_564


title: nuclei_562


title: nuclei_550


title: nuclei_557


title: nuclei_555


title: nuclei_560


title: nuclei_559


title: nuclei_552


title: nuclei_546


title: nuclei_554


title: nuclei_545


title: nuclei_551


title: nuclei_544


title: nuclei_548


title: nuclei_549


title: nuclei_542


title: nuclei_565


title: nuclei_543


title: nuclei_541


title: nuclei_539


title: nuclei_547


title: nuclei_538


title: nuclei_556


title: nuclei_540


title: nuclei_512


title: nuclei_511


title: nuclei_518


title: nuclei_520


title: nuclei_536


title: nuclei_533


title: nuclei_537


title: nuclei_524


title: nuclei_523


title: nuclei_514


title: nuclei_526


title: nuclei_527


title: nuclei_516


title: nuclei_510


title: nuclei_513


title: nuclei_528


title: nuclei_517


title: nuclei_532


title: nuclei_525


title: nuclei_580


title: nuclei_535


title: nuclei_507


title: nuclei_509


title: nuclei_519


title: nuclei_508


title: nuclei_506


title: nuclei_505


title: nuclei_515


title: nuclei_455


title: nuclei_456


title: nuclei_453


title: nuclei_439


title: nuclei_440


title: nuclei_437


title: nuclei_435


title: nuclei_452


title: nuclei_433


title: nuclei_454


title: nuclei_445


title: nuclei_438


title: nuclei_447


title: nuclei_436


title: nuclei_430


title: nuclei_432


title: nuclei_429


title: nuclei_558


title: nuclei_426


title: nuclei_442


title: nuclei_422


title: nuclei_418


title: nuclei_423


title: nuclei_424


title: nuclei_420


title: nuclei_421


title: nuclei_428


title: nuclei_416


title: nuclei_450


title: nuclei_431


title: nuclei_417


title: nuclei_414


title: nuclei_415


title: nuclei_410


title: nuclei_413


title: nuclei_402


title: nuclei_409


title: nuclei_404


title: nuclei_412


title: nuclei_411


title: nuclei_407


title: nuclei_406


title: nuclei_401


title: nuclei_405


title: nuclei_408


title: nuclei_400


title: nuclei_397


title: nuclei_398


title: nuclei_399


title: nuclei_393


title: nuclei_394


title: nuclei_395


title: nuclei_391


title: nuclei_396


title: nuclei_388


title: nuclei_403


title: nuclei_392


title: nuclei_387


title: nuclei_389


title: nuclei_385


title: nuclei_384


title: nuclei_390


title: nuclei_382


title: nuclei_381


title: nuclei_380


title: nuclei_379


title: nuclei_374


title: nuclei_378


title: nuclei_376


title: nuclei_377


title: nuclei_373


title: nuclei_434


title: nuclei_366


title: nuclei_367


title: nuclei_369


title: nuclei_365


title: nuclei_386


title: nuclei_368


title: nuclei_370


title: nuclei_361


title: nuclei_359


title: nuclei_364


title: nuclei_360


title: nuclei_358


title: nuclei_357


title: nuclei_363


title: nuclei_383


title: nuclei_354


title: nuclei_355


title: nuclei_356


title: NCKD Input

description: Negative control sh RNA transduced cells

tag: cell line

value: Jurkat

tag: chip antibody

value: none (input)


title: nuclei_353


title: nuclei_351


title: nuclei_352


title: nuclei_347


title: nuclei_346


title: nuclei_349


title: nuclei_345


title: nuclei_375


title: nuclei_344


title: nuclei_343


title: nuclei_340


title: nuclei_338


title: nuclei_339


title: nuclei_341


title: nuclei_342


title: nuclei_337


title: nuclei_331


title: nuclei_332


title: nuclei_336


title: nuclei_330


title: nuclei_335


title: nuclei_333


title: nuclei_329


title: nuclei_327


title: nuclei_328


title: nuclei_325


title: nuclei_326


title: nuclei_334


title: nuclei_350


title: nuclei_348


title: nuclei_319


title: nuclei_323


title: nuclei_318


title: nuclei_320


title: nuclei_316


title: nuclei_317


title: nuclei_313


title: nuclei_315


title: nuclei_311


title: nuclei_312


title: nuclei_314


title: nuclei_310


title: nuclei_309


title: nuclei_308


title: nuclei_307


title: nuclei_306


title: nuclei_305


title: nuclei_324


title: nuclei_295


title: nuclei_303


title: nuclei_298


title: nuclei_297


title: nuclei_304


title: nuclei_299


title: nuclei_300


title: nuclei_296


title: nuclei_301


title: nuclei_321


title: nuclei_294


title: nuclei_322


title: nuclei_288


title: nuclei_293


title: nuclei_287


title: nuclei_289


title: nuclei_292


title: nuclei_285


title: nuclei_284


title: nuclei_286


title: nuclei_283


title: nuclei_282


title: nuclei_281


title: nuclei_279


title: nuclei_280


title: nuclei_278


title: nuclei_290


title: nuclei_291


title: nuclei_275


title: nuclei_276


title: nuclei_277


title: nuclei_274


title: nuclei_272


title: nuclei_273


title: nuclei_302


title: nuclei_265


title: nuclei_269


title: nuclei_266


title: nuclei_267


title: nuclei_271


title: nuclei_270


title: nuclei_268


title: nuclei_262


title: nuclei_261


title: Pool_99_AD002_indexed


title: Pool_99_AD006_indexed


title: Pool_999_AD006_indexed


title: nuclei_263


title: Pool_998_AD002_indexed


title: Pool_996_AD006_indexed


title: Pool_997_AD006_indexed


title: Pool_997_AD002_indexed


title: Pool_994_AD002_indexed


title: Pool_995_AD002_indexed


title: Pool_994_AD006_indexed


title: Pool_996_AD002_indexed


title: Pool_995_AD006_indexed


title: Pool_9_AD002_indexed


title: Pool_990_AD002_indexed


title: Pool_992_AD002_indexed


title: Pool_991_AD002_indexed


title: Pool_991_AD006_indexed


title: Pool_98_AD002_indexed


title: Pool_989_AD006_indexed


title: Pool_993_AD002_indexed


title: Pool_998_AD006_indexed


title: Pool_999_AD002_indexed


title: Pool_989_AD002_indexed


title: Pool_98_AD006_indexed


title: Pool_988_AD002_indexed


title: Pool_988_AD006_indexed


title: Pool_987_AD006_indexed


title: Pool_987_AD002_indexed


title: nuclei_264


title: Pool_986_AD002_indexed


title: Pool_985_AD006_indexed


title: Pool_985_AD002_indexed


title: Pool_984_AD002_indexed


title: Pool_983_AD002_indexed


title: Pool_983_AD006_indexed


title: Pool_982_AD006_indexed


title: Pool_992_AD006_indexed


title: Pool_990_AD006_indexed


title: Pool_982_AD002_indexed


title: Pool_986_AD006_indexed


title: Pool_993_AD006_indexed


title: Pool_981_AD006_indexed


title: Pool_980_AD006_indexed


title: Pool_980_AD002_indexed


title: Pool_978_AD006_indexed


title: Pool_979_AD002_indexed


title: Pool_984_AD006_indexed


title: Pool_981_AD002_indexed


title: Pool_978_AD002_indexed


title: Pool_97_AD006_indexed


title: Pool_977_AD006_indexed


title: Pool_975_AD006_indexed


title: Pool_974_AD002_indexed


title: Pool_976_AD002_indexed


title: Pool_974_AD006_indexed


title: Pool_975_AD002_indexed


title: Pool_976_AD006_indexed


title: Pool_97_AD002_indexed


title: Pool_972_AD006_indexed


title: Pool_972_AD002_indexed


title: Pool_971_AD006_indexed


title: Pool_973_AD006_indexed


title: Pool_970_AD006_indexed


title: Pool_971_AD002_indexed


title: Pool_970_AD002_indexed


title: Pool_96_AD006_indexed


title: Pool_969_AD002_indexed


title: Pool_968_AD006_indexed


title: Pool_979_AD006_indexed


title: Pool_96_AD002_indexed


title: Pool_968_AD002_indexed


title: Pool_977_AD002_indexed


title: Pool_965_AD002_indexed


title: Pool_966_AD002_indexed


title: Pool_965_AD006_indexed


title: Pool_964_AD006_indexed


title: Pool_966_AD006_indexed


title: Pool_973_AD002_indexed


title: Pool_967_AD006_indexed


title: Pool_962_AD006_indexed


title: Pool_967_AD002_indexed


title: Pool_963_AD002_indexed


title: Pool_964_AD002_indexed


title: Pool_963_AD006_indexed


title: Pool_961_AD002_indexed


title: Pool_960_AD002_indexed


title: Pool_961_AD006_indexed


title: Pool_960_AD006_indexed


title: Pool_95_AD006_indexed


title: Pool_959_AD002_indexed


title: Pool_969_AD006_indexed


title: Pool_958_AD006_indexed


title: Pool_959_AD006_indexed


title: Pool_958_AD002_indexed


title: Pool_957_AD006_indexed


title: Pool_957_AD002_indexed


title: Pool_956_AD006_indexed


title: Pool_956_AD002_indexed


title: Pool_962_AD002_indexed


title: Pool_955_AD006_indexed


title: Pool_955_AD002_indexed


title: Pool_954_AD006_indexed


title: Pool_954_AD002_indexed


title: Pool_952_AD002_indexed


title: Pool_951_AD002_indexed


title: Pool_952_AD006_indexed


title: Pool_953_AD006_indexed


title: Pool_94_AD006_indexed


title: Pool_95_AD002_indexed


title: Pool_950_AD002_indexed


title: Pool_950_AD006_indexed


title: Pool_951_AD006_indexed


title: Pool_94_AD002_indexed


title: Pool_949_AD006_indexed


title: Pool_948_AD002_indexed


title: Pool_949_AD002_indexed


title: Pool_946_AD006_indexed


title: Pool_945_AD006_indexed


title: Pool_945_AD002_indexed


title: Pool_944_AD006_indexed


title: Pool_947_AD006_indexed


title: Pool_944_AD002_indexed


title: Pool_947_AD002_indexed


title: Pool_942_AD006_indexed


title: Pool_941_AD006_indexed


title: Pool_943_AD006_indexed


title: Pool_941_AD002_indexed


title: Pool_940_AD006_indexed


title: Pool_943_AD002_indexed


title: Pool_942_AD002_indexed


title: Pool_93_AD002_indexed


title: Pool_93_AD006_indexed


title: Pool_937_AD006_indexed


title: Pool_940_AD002_indexed


title: Pool_948_AD006_indexed


title: Pool_935_AD002_indexed


title: Pool_939_AD006_indexed


title: Pool_939_AD002_indexed


title: Pool_938_AD002_indexed


title: Pool_938_AD006_indexed


title: Pool_934_AD006_indexed


title: Pool_933_AD002_indexed


title: Pool_937_AD002_indexed


title: Pool_92_AD002_indexed


title: Pool_930_AD006_indexed


title: Pool_92_AD006_indexed


title: Pool_931_AD002_indexed


title: Pool_935_AD006_indexed


title: Pool_932_AD006_indexed


title: Pool_936_AD006_indexed


title: Pool_932_AD002_indexed


title: Pool_931_AD006_indexed


title: Pool_929_AD002_indexed


title: Pool_936_AD002_indexed


title: Pool_930_AD002_indexed


title: Pool_933_AD006_indexed


title: Pool_929_AD006_indexed


title: Pool_927_AD002_indexed


title: Pool_934_AD002_indexed


title: Pool_928_AD006_indexed


title: Pool_946_AD002_indexed


title: Pool_925_AD002_indexed


title: Pool_925_AD006_indexed


title: Pool_926_AD002_indexed


title: Pool_923_AD002_indexed


title: Pool_926_AD006_indexed


title: Pool_924_AD006_indexed


title: Pool_922_AD006_indexed


title: Pool_923_AD006_indexed


title: Pool_928_AD002_indexed


title: Pool_920_AD006_indexed


title: Pool_922_AD002_indexed


title: Pool_920_AD002_indexed


title: Pool_927_AD006_indexed


title: Pool_91_AD002_indexed


title: Pool_918_AD006_indexed


title: Pool_919_AD006_indexed


title: Pool_919_AD002_indexed


title: Pool_917_AD002_indexed


title: Pool_915_AD002_indexed


title: Pool_916_AD002_indexed


title: Pool_916_AD006_indexed


title: Pool_915_AD006_indexed


title: Pool_914_AD006_indexed


title: Pool_917_AD006_indexed


title: Pool_953_AD002_indexed


title: Pool_913_AD006_indexed


title: Pool_912_AD010_indexed


title: Pool_912_AD008_indexed


title: Pool_913_AD002_indexed


title: Pool_911_AD008_indexed


title: Pool_910_AD010_indexed


title: Pool_921_AD006_indexed


title: Pool_910_AD008_indexed


title: Pool_91_AD006_indexed


title: Pool_924_AD002_indexed


title: Pool_90_AD006_indexed


title: Pool_90_AD002_indexed


title: Pool_918_AD002_indexed


title: Pool_909_AD010_indexed


title: Pool_909_AD008_indexed


title: Pool_908_AD010_indexed


title: Pool_914_AD002_indexed


title: Pool_908_AD008_indexed


title: Pool_921_AD002_indexed


title: Pool_906_AD010_indexed


title: Pool_907_AD010_indexed


title: Pool_906_AD008_indexed


title: Pool_907_AD008_indexed


title: Pool_905_AD010_indexed


title: Pool_903_AD010_indexed


title: Pool_904_AD008_indexed


title: Pool_905_AD008_indexed


title: Pool_904_AD010_indexed


title: Pool_903_AD008_indexed


title: Pool_902_AD008_indexed


title: Pool_902_AD010_indexed


title: Pool_8_AD006_indexed


title: Pool_901_AD008_indexed


title: Pool_901_AD010_indexed


title: Pool_8_AD002_indexed


title: Pool_89_AD006_indexed


title: Pool_89_AD002_indexed


title: Pool_899_AD010_indexed


title: Pool_899_AD008_indexed


title: Pool_896_AD010_indexed


title: Pool_895_AD008_indexed


title: Pool_898_AD008_indexed


title: Pool_898_AD010_indexed


title: Pool_897_AD010_indexed


title: Pool_897_AD008_indexed


title: Pool_896_AD008_indexed


title: Pool_894_AD010_indexed


title: Pool_895_AD010_indexed


title: Pool_893_AD010_indexed


title: Pool_900_AD008_indexed


title: Pool_900_AD010_indexed


title: Pool_891_AD010_indexed


title: Pool_892_AD010_indexed


title: Pool_890_AD010_indexed


title: Pool_891_AD008_indexed


title: Pool_890_AD008_indexed


title: Pool_88_AD002_indexed


title: Pool_88_AD006_indexed


title: Pool_892_AD008_indexed


title: Pool_889_AD010_indexed


title: Pool_888_AD010_indexed


title: Pool_888_AD008_indexed


title: Pool_887_AD010_indexed


title: Pool_889_AD008_indexed


title: Pool_886_AD008_indexed


title: Pool_887_AD008_indexed


title: Pool_886_AD010_indexed


title: Pool_894_AD008_indexed


title: Pool_885_AD008_indexed


title: Pool_893_AD008_indexed


title: Pool_883_AD010_indexed


title: Pool_882_AD008_indexed


title: Pool_881_AD008_indexed


title: Pool_884_AD008_indexed


title: Pool_880_AD008_indexed


title: Pool_885_AD010_indexed


title: Pool_880_AD010_indexed


title: Pool_881_AD010_indexed


title: Pool_87_AD006_indexed


title: Pool_878_AD010_indexed


title: Pool_87_AD002_indexed


title: Pool_879_AD008_indexed


title: Pool_878_AD008_indexed


title: Pool_877_AD008_indexed


title: Pool_877_AD010_indexed


title: Pool_879_AD010_indexed


title: Pool_875_AD010_indexed


title: Pool_876_AD010_indexed


title: Pool_875_AD008_indexed


title: Pool_876_AD008_indexed


title: Pool_872_AD010_indexed


title: Pool_873_AD010_indexed


title: Pool_873_AD008_indexed


title: Pool_874_AD010_indexed


title: Pool_874_AD008_indexed


title: Pool_883_AD008_indexed


title: Pool_872_AD008_indexed


title: Pool_882_AD010_indexed


title: Pool_870_AD010_indexed


title: Pool_871_AD008_indexed


title: Pool_870_AD008_indexed


title: Pool_86_AD002_indexed


title: Pool_867_AD008_indexed


title: Pool_869_AD008_indexed


title: Pool_86_AD006_indexed


title: Pool_869_AD010_indexed


title: Pool_864_AD008_indexed


title: Pool_865_AD010_indexed


title: Pool_865_AD008_indexed


title: Pool_864_AD010_indexed


title: Pool_866_AD008_indexed


title: Pool_866_AD010_indexed


title: Pool_863_AD008_indexed


title: Pool_862_AD008_indexed


title: Pool_867_AD010_indexed


title: Pool_860_AD010_indexed


title: Pool_85_AD002_indexed


title: Pool_861_AD010_indexed


title: Pool_862_AD010_indexed


title: Pool_861_AD008_indexed


title: Pool_860_AD008_indexed


title: Pool_859_AD010_indexed


title: Pool_85_AD006_indexed


title: Pool_858_AD010_indexed


title: Pool_859_AD008_indexed


title: Pool_858_AD008_indexed


title: Pool_871_AD010_indexed


title: Pool_868_AD008_indexed


title: Pool_868_AD010_indexed


title: Pool_863_AD010_indexed


title: Pool_857_AD010_indexed


title: Pool_857_AD008_indexed


title: Pool_855_AD008_indexed


title: Pool_856_AD008_indexed


title: Pool_855_AD010_indexed


title: Pool_856_AD010_indexed


title: Pool_854_AD008_indexed


title: Pool_854_AD010_indexed


title: Pool_853_AD008_indexed


title: Pool_852_AD008_indexed


title: Pool_851_AD010_indexed


title: Pool_847_AD010_indexed


title: Pool_852_AD010_indexed


title: Pool_84_AD002_indexed


title: Pool_848_AD010_indexed


title: Pool_848_AD008_indexed


title: Pool_84_AD006_indexed


title: Pool_850_AD010_indexed


title: Pool_850_AD008_indexed


title: Pool_849_AD010_indexed


title: Pool_847_AD008_indexed


title: Pool_846_AD008_indexed


title: Pool_845_AD010_indexed


title: Pool_844_AD008_indexed


title: Pool_844_AD010_indexed


title: Pool_845_AD008_indexed


title: Pool_846_AD010_indexed


title: Pool_843_AD010_indexed


title: Pool_853_AD010_indexed


title: Pool_842_AD008_indexed


title: Pool_843_AD008_indexed


title: Pool_841_AD010_indexed


title: Pool_841_AD008_indexed


title: Pool_842_AD010_indexed


title: Pool_840_AD006_indexed


title: Pool_840_AD002_indexed


title: Pool_851_AD008_indexed


title: Pool_838_AD006_indexed


title: Pool_839_AD002_indexed


title: Pool_836_AD006_indexed


title: Pool_83_AD002_indexed


title: Pool_835_AD006_indexed


title: Pool_836_AD002_indexed


title: Pool_835_AD002_indexed


title: Pool_834_AD002_indexed


title: Pool_837_AD006_indexed


title: Pool_837_AD002_indexed


title: Pool_833_AD002_indexed


title: Pool_834_AD006_indexed


title: Pool_832_AD006_indexed


title: Pool_832_AD002_indexed


title: Pool_833_AD006_indexed


title: Pool_831_AD006_indexed


title: Pool_831_AD002_indexed


title: Pool_830_AD006_indexed


title: Pool_83_AD006_indexed


title: Pool_830_AD002_indexed


title: Pool_829_AD006_indexed


title: Pool_82_AD006_indexed


title: Pool_829_AD002_indexed


title: Pool_82_AD002_indexed


title: Pool_828_AD006_indexed


title: Pool_839_AD006_indexed


title: Pool_838_AD002_indexed


title: Pool_827_AD002_indexed


title: Pool_826_AD006_indexed


title: Pool_821_AD002_indexed


title: Pool_823_AD006_indexed


title: Pool_822_AD006_indexed


title: Pool_822_AD002_indexed


title: Pool_823_AD002_indexed


title: Pool_821_AD006_indexed


title: Pool_824_AD002_indexed


title: Pool_828_AD002_indexed


title: Pool_820_AD002_indexed


title: Pool_81_AD006_indexed


title: Pool_820_AD006_indexed


title: Pool_819_AD002_indexed


title: Pool_819_AD006_indexed


title: Pool_827_AD006_indexed


title: Pool_817_AD006_indexed


title: Pool_818_AD006_indexed


title: Pool_816_AD002_indexed


title: Pool_816_AD006_indexed


title: Pool_817_AD002_indexed


title: Pool_815_AD002_indexed


title: Pool_815_AD006_indexed


title: Pool_814_AD006_indexed


title: Pool_814_AD002_indexed


title: Pool_813_AD006_indexed


title: Pool_825_AD006_indexed


title: Pool_813_AD002_indexed


title: Pool_824_AD006_indexed


title: Pool_812_AD006_indexed


title: Pool_811_AD006_indexed


title: Pool_811_AD002_indexed


title: Pool_818_AD002_indexed


title: Pool_810_AD006_indexed


title: Pool_80_AD006_indexed


title: Pool_80_AD002_indexed


title: Pool_809_AD002_indexed


title: Pool_810_AD002_indexed


title: Pool_809_AD006_indexed


title: Pool_808_AD006_indexed


title: Pool_808_AD002_indexed


title: Pool_806_AD002_indexed


title: Pool_807_AD002_indexed


title: Pool_806_AD006_indexed


title: Pool_805_AD006_indexed


title: Pool_804_AD002_indexed


title: Pool_804_AD006_indexed


title: Pool_803_AD006_indexed


title: Pool_803_AD002_indexed


title: Pool_805_AD002_indexed


title: Pool_802_AD006_indexed


title: Pool_802_AD002_indexed


title: Pool_799_AD006_indexed


title: Pool_7_AD002_indexed


title: Pool_800_AD006_indexed


title: Pool_79_AD006_indexed


title: Pool_801_AD006_indexed


title: Pool_799_AD002_indexed


title: Pool_801_AD002_indexed


title: Pool_798_AD002_indexed


title: Pool_798_AD006_indexed


title: Pool_800_AD002_indexed


title: Pool_791_AD006_indexed


title: Pool_797_AD002_indexed


title: Pool_794_AD002_indexed


title: Pool_792_AD006_indexed


title: Pool_793_AD006_indexed


title: Pool_795_AD002_indexed


title: Pool_792_AD002_indexed


title: Pool_796_AD006_indexed


title: Pool_78_AD006_indexed


title: Pool_790_AD006_indexed


title: Pool_796_AD002_indexed


title: Pool_793_AD002_indexed


title: Pool_794_AD006_indexed


title: Pool_797_AD006_indexed


title: Pool_807_AD006_indexed


title: Pool_789_AD006_indexed


title: Pool_795_AD006_indexed


title: Pool_787_AD002_indexed


title: Pool_788_AD006_indexed


title: Pool_784_AD006_indexed


title: Pool_787_AD006_indexed


title: Pool_788_AD002_indexed


title: Pool_783_AD006_indexed


title: Pool_784_AD002_indexed


title: Pool_789_AD002_indexed


title: Pool_785_AD006_indexed


title: Pool_783_AD002_indexed


title: Pool_78_AD002_indexed


title: Pool_782_AD006_indexed


title: Pool_780_AD006_indexed


title: Pool_781_AD002_indexed


title: Pool_781_AD006_indexed


title: Pool_782_AD002_indexed


title: Pool_77_AD006_indexed


title: Pool_779_AD002_indexed


title: Pool_7_AD006_indexed


title: Pool_779_AD006_indexed


title: Pool_77_AD002_indexed


title: Pool_777_AD002_indexed


title: Pool_778_AD006_indexed


title: Pool_777_AD006_indexed


title: Pool_776_AD006_indexed


title: Pool_778_AD002_indexed


title: Pool_776_AD002_indexed


title: Pool_786_AD006_indexed


title: Pool_775_AD006_indexed


title: Pool_775_AD002_indexed


title: Pool_774_AD006_indexed


title: Pool_773_AD006_indexed


title: Pool_774_AD002_indexed


title: Pool_772_AD002_indexed


title: Pool_780_AD002_indexed


title: Pool_773_AD002_indexed


title: Pool_771_AD006_indexed


title: Pool_772_AD006_indexed


title: Pool_791_AD002_indexed


title: Pool_770_AD006_indexed


title: Pool_769_AD002_indexed


title: Pool_771_AD002_indexed


title: Pool_76_AD006_indexed


title: Pool_76_AD002_indexed


title: Pool_768_AD010_indexed


title: Pool_769_AD006_indexed


title: Pool_766_AD010_indexed


title: Pool_764_AD008_indexed


title: Pool_764_AD010_indexed


title: Pool_767_AD008_indexed


title: Pool_765_AD010_indexed


title: Pool_763_AD008_indexed


title: Pool_766_AD008_indexed


title: Pool_765_AD008_indexed


title: Pool_762_AD010_indexed


title: Pool_761_AD008_indexed


title: Pool_760_AD010_indexed


title: Pool_760_AD008_indexed


title: Pool_75_AD006_indexed


title: Pool_75_AD002_indexed


title: Pool_759_AD010_indexed


title: Pool_761_AD010_indexed


title: Pool_768_AD008_indexed


title: Pool_758_AD010_indexed


title: Pool_767_AD010_indexed


title: Pool_758_AD008_indexed


title: Pool_757_AD010_indexed


title: Pool_763_AD010_indexed


title: Pool_756_AD010_indexed


title: Pool_756_AD008_indexed


title: Pool_762_AD008_indexed


title: Pool_753_AD008_indexed


title: Pool_754_AD010_indexed


title: Pool_754_AD008_indexed


title: Pool_755_AD008_indexed


title: Pool_752_AD008_indexed


title: Pool_752_AD010_indexed


title: Pool_750_AD008_indexed


title: Pool_757_AD008_indexed


title: Pool_751_AD008_indexed


title: Pool_750_AD010_indexed


title: Pool_751_AD010_indexed


title: Pool_755_AD010_indexed


title: Pool_759_AD008_indexed


title: Pool_749_AD010_indexed


title: Pool_74_AD002_indexed


title: Pool_747_AD010_indexed


title: Pool_748_AD008_indexed


title: Pool_748_AD010_indexed


title: Pool_747_AD008_indexed


title: Pool_746_AD008_indexed


title: Pool_746_AD010_indexed


title: Pool_745_AD010_indexed


title: Pool_753_AD010_indexed


title: Pool_745_AD008_indexed


title: Pool_744_AD010_indexed


title: Pool_744_AD008_indexed


title: Pool_743_AD010_indexed


title: Pool_743_AD008_indexed


title: Pool_74_AD006_indexed


title: Pool_742_AD010_indexed


title: Pool_741_AD010_indexed


title: Pool_741_AD008_indexed


title: Pool_740_AD008_indexed


title: Pool_73_AD002_indexed


title: Pool_73_AD006_indexed


title: Pool_740_AD010_indexed


title: Pool_739_AD010_indexed


title: Pool_739_AD008_indexed


title: Pool_738_AD010_indexed


title: Pool_749_AD008_indexed


title: Pool_738_AD008_indexed


title: Pool_737_AD010_indexed


title: Pool_737_AD008_indexed


title: Pool_736_AD010_indexed


title: Pool_735_AD010_indexed


title: Pool_736_AD008_indexed


title: Pool_735_AD008_indexed


title: Pool_734_AD010_indexed


title: Pool_733_AD010_indexed


title: Pool_734_AD008_indexed


title: Pool_733_AD008_indexed


title: Pool_732_AD008_indexed


title: Pool_732_AD010_indexed


title: Pool_742_AD008_indexed


title: Pool_731_AD010_indexed


title: Pool_731_AD008_indexed


title: Pool_729_AD008_indexed


title: Pool_729_AD010_indexed


title: Pool_72_AD002_indexed


title: Pool_730_AD008_indexed


title: Pool_727_AD010_indexed


title: Pool_728_AD008_indexed


title: Pool_728_AD010_indexed


title: Pool_72_AD006_indexed


title: Pool_727_AD008_indexed


title: Pool_725_AD010_indexed


title: Pool_725_AD008_indexed


title: Pool_726_AD010_indexed


title: Pool_724_AD010_indexed


title: Pool_724_AD008_indexed


title: Pool_722_AD010_indexed


title: Pool_723_AD008_indexed


title: Pool_723_AD010_indexed


title: Pool_721_AD010_indexed


title: Pool_720_AD010_indexed


title: Pool_722_AD008_indexed


title: Pool_720_AD008_indexed


title: Pool_71_AD006_indexed


title: Pool_71_AD002_indexed


title: Pool_719_AD008_indexed


title: Pool_730_AD010_indexed


title: Pool_718_AD010_indexed


title: Pool_719_AD010_indexed


title: Pool_726_AD008_indexed


title: Pool_721_AD008_indexed


title: Pool_717_AD008_indexed


title: Pool_716_AD010_indexed


title: Pool_714_AD008_indexed


title: Pool_715_AD008_indexed


title: Pool_715_AD010_indexed


title: Pool_716_AD008_indexed


title: Pool_713_AD010_indexed


title: Pool_714_AD010_indexed


title: Pool_711_AD010_indexed


title: Pool_712_AD010_indexed


title: Pool_710_AD010_indexed


title: Pool_713_AD008_indexed


title: Pool_710_AD008_indexed


title: Pool_711_AD008_indexed


title: Pool_70_AD006_indexed


title: Pool_70_AD002_indexed


title: Pool_718_AD008_indexed


title: Pool_717_AD010_indexed


title: Pool_709_AD010_indexed


title: Pool_709_AD008_indexed


title: Pool_706_AD008_indexed


title: Pool_707_AD008_indexed


title: Pool_708_AD008_indexed


title: Pool_707_AD010_indexed


title: Pool_705_AD010_indexed


title: Pool_706_AD010_indexed


title: Pool_705_AD008_indexed


title: Pool_704_AD010_indexed


title: Pool_712_AD008_indexed


title: Pool_704_AD008_indexed


title: Pool_703_AD008_indexed


title: Pool_703_AD010_indexed


title: Pool_702_AD010_indexed


title: Pool_702_AD008_indexed


title: Pool_69_AD002_indexed


title: Pool_700_AD008_indexed


title: Pool_701_AD008_indexed


title: Pool_6_AD002_indexed


title: Pool_699_AD010_indexed


title: Pool_69_AD006_indexed


title: Pool_699_AD008_indexed


title: Pool_701_AD010_indexed


title: Pool_697_AD010_indexed


title: Pool_698_AD010_indexed


title: Pool_697_AD008_indexed


title: Pool_693_AD010_indexed


title: Pool_695_AD008_indexed


title: Pool_692_AD010_indexed


title: Pool_694_AD008_indexed


title: Pool_695_AD010_indexed


title: Pool_694_AD010_indexed


title: Pool_692_AD008_indexed


title: Pool_696_AD008_indexed


title: Pool_691_AD010_indexed


title: Pool_696_AD010_indexed


title: Pool_700_AD010_indexed


title: Pool_693_AD008_indexed


title: Pool_691_AD008_indexed


title: Pool_690_AD008_indexed


title: Pool_690_AD010_indexed


title: Pool_698_AD008_indexed


title: Pool_689_AD010_indexed


title: Pool_689_AD008_indexed


title: Pool_688_AD010_indexed


title: Pool_687_AD010_indexed


title: Pool_688_AD008_indexed


title: Pool_687_AD008_indexed


title: Pool_685_AD008_indexed


title: Pool_686_AD008_indexed


title: Pool_686_AD010_indexed


title: Pool_684_AD010_indexed


title: Pool_684_AD008_indexed


title: Pool_683_AD010_indexed


title: Pool_68_AD006_indexed


title: Pool_681_AD010_indexed


title: Pool_682_AD008_indexed


title: Pool_680_AD008_indexed


title: Pool_681_AD008_indexed


title: Pool_680_AD010_indexed


title: Pool_679_AD010_indexed


title: Pool_682_AD010_indexed


title: Pool_67_AD002_indexed


title: Pool_67_AD006_indexed


title: Pool_676_AD010_indexed


title: Pool_677_AD010_indexed


title: Pool_679_AD008_indexed


title: Pool_678_AD010_indexed


title: Pool_678_AD008_indexed


title: Pool_685_AD010_indexed


title: Pool_677_AD008_indexed


title: Pool_676_AD008_indexed


title: Pool_674_AD008_indexed


title: Pool_683_AD008_indexed


title: Pool_672_AD006_indexed


title: Pool_673_AD008_indexed


title: Pool_674_AD010_indexed


title: Pool_672_AD002_indexed


title: Pool_671_AD006_indexed


title: Pool_671_AD002_indexed


title: Pool_670_AD002_indexed


title: Pool_670_AD006_indexed


title: Pool_66_AD006_indexed


title: Pool_669_AD002_indexed


title: Pool_669_AD006_indexed


title: Pool_668_AD002_indexed


title: Pool_666_AD006_indexed


title: Pool_667_AD006_indexed


title: Pool_675_AD010_indexed


title: Pool_675_AD008_indexed


title: Pool_667_AD002_indexed


title: Pool_664_AD002_indexed


title: Pool_664_AD006_indexed


title: Pool_662_AD006_indexed


title: Pool_661_AD006_indexed


title: Pool_663_AD002_indexed


title: Pool_665_AD006_indexed


title: Pool_665_AD002_indexed


title: Pool_662_AD002_indexed


title: Pool_660_AD006_indexed


title: Pool_673_AD010_indexed


title: Pool_660_AD002_indexed


title: Pool_659_AD002_indexed


title: Pool_65_AD006_indexed


title: Pool_658_AD006_indexed


title: Pool_668_AD006_indexed


title: Pool_666_AD002_indexed


title: Pool_657_AD006_indexed


title: Pool_659_AD006_indexed


title: Pool_657_AD002_indexed


title: Pool_656_AD006_indexed


title: Pool_655_AD006_indexed


title: Pool_655_AD002_indexed


title: Pool_656_AD002_indexed


title: Pool_654_AD006_indexed


title: Pool_658_AD002_indexed


title: Pool_653_AD006_indexed


title: Pool_654_AD002_indexed


title: Pool_652_AD006_indexed


title: Pool_653_AD002_indexed


title: Pool_661_AD002_indexed


title: Pool_652_AD002_indexed


title: Pool_651_AD006_indexed


title: Pool_650_AD002_indexed


title: Pool_64_AD006_indexed


title: Pool_650_AD006_indexed


title: Pool_651_AD002_indexed


title: Pool_649_AD006_indexed


title: Pool_649_AD002_indexed


title: Pool_648_AD006_indexed


title: Pool_648_AD002_indexed


title: Pool_647_AD002_indexed


title: Pool_646_AD002_indexed


title: Pool_645_AD002_indexed


title: Pool_643_AD002_indexed


title: Pool_644_AD002_indexed


title: Pool_646_AD006_indexed


title: Pool_645_AD006_indexed


title: Pool_641_AD002_indexed


title: Pool_640_AD006_indexed


title: Pool_641_AD006_indexed


title: Pool_640_AD002_indexed


title: Pool_642_AD006_indexed


title: Pool_63_AD006_indexed


title: Pool_63_AD002_indexed


title: Pool_64_AD002_indexed


title: Pool_643_AD006_indexed


title: Pool_639_AD006_indexed


title: Pool_639_AD002_indexed


title: Pool_638_AD006_indexed


title: Pool_638_AD002_indexed


title: Pool_647_AD006_indexed


title: Pool_637_AD006_indexed


title: Pool_636_AD002_indexed


title: Pool_637_AD002_indexed


title: Pool_636_AD006_indexed


title: Pool_635_AD002_indexed


title: Pool_635_AD006_indexed


title: Pool_634_AD006_indexed


title: Pool_634_AD002_indexed


title: Pool_633_AD002_indexed


title: Pool_642_AD002_indexed


title: Pool_630_AD006_indexed


title: Pool_632_AD002_indexed


title: Pool_632_AD006_indexed


title: Pool_631_AD006_indexed


title: Pool_630_AD002_indexed


title: Pool_626_AD002_indexed


title: Pool_62_AD002_indexed


title: Pool_62_AD006_indexed


title: Pool_626_AD006_indexed


title: Pool_628_AD002_indexed


title: Pool_629_AD006_indexed


title: Pool_627_AD006_indexed


title: Pool_627_AD002_indexed


title: Pool_629_AD002_indexed


title: Pool_628_AD006_indexed


title: Pool_625_AD006_indexed


title: Pool_625_AD002_indexed


title: Pool_624_AD002_indexed


title: Pool_624_AD006_indexed


title: Pool_623_AD006_indexed


title: Pool_622_AD006_indexed


title: Pool_633_AD006_indexed


title: Pool_621_AD002_indexed


title: Pool_621_AD006_indexed


title: Pool_620_AD006_indexed


title: Pool_631_AD002_indexed


title: Pool_620_AD002_indexed


title: Pool_61_AD006_indexed


title: Pool_61_AD002_indexed


title: Pool_619_AD002_indexed


title: Pool_618_AD006_indexed


title: Pool_617_AD006_indexed


title: Pool_617_AD002_indexed


title: Pool_616_AD006_indexed


title: Pool_615_AD006_indexed


title: Pool_616_AD002_indexed


title: Pool_612_AD002_indexed


title: Pool_612_AD006_indexed


title: Pool_615_AD002_indexed


title: Pool_611_AD006_indexed


title: Pool_609_AD002_indexed


title: Pool_60_AD006_indexed


title: Pool_610_AD006_indexed


title: Pool_609_AD006_indexed


title: Pool_613_AD006_indexed


title: Pool_608_AD006_indexed


title: Pool_606_AD006_indexed


title: Pool_607_AD006_indexed


title: Pool_607_AD002_indexed


title: Pool_614_AD002_indexed


title: Pool_618_AD002_indexed


title: Pool_603_AD002_indexed


title: Pool_613_AD002_indexed


title: Pool_602_AD006_indexed


title: Pool_601_AD002_indexed


title: Pool_601_AD006_indexed


title: Pool_602_AD002_indexed


title: Pool_603_AD006_indexed


title: Pool_600_AD002_indexed


title: Pool_605_AD006_indexed


title: Pool_610_AD002_indexed


title: Pool_5_AD002_indexed


title: Pool_605_AD002_indexed


title: Pool_59_AD002_indexed


title: Pool_59_AD006_indexed


title: Pool_599_AD002_indexed


title: Pool_597_AD006_indexed


title: Pool_596_AD006_indexed


title: Pool_596_AD002_indexed


title: Pool_598_AD002_indexed


title: Pool_597_AD002_indexed


title: Pool_595_AD006_indexed


title: Pool_598_AD006_indexed


title: Pool_600_AD006_indexed


title: Pool_594_AD002_indexed


title: Pool_595_AD002_indexed


title: Pool_593_AD006_indexed


title: Pool_594_AD006_indexed


title: Pool_5_AD006_indexed


title: Pool_592_AD002_indexed


title: Pool_592_AD006_indexed


title: Pool_591_AD006_indexed


title: Pool_589_AD006_indexed


title: Pool_599_AD006_indexed


title: Pool_591_AD002_indexed


title: Pool_590_AD006_indexed


title: Pool_58_AD006_indexed


title: Pool_590_AD002_indexed


title: Pool_589_AD002_indexed


title: Pool_593_AD002_indexed


title: Pool_587_AD006_indexed


title: Pool_588_AD006_indexed


title: Pool_584_AD002_indexed


title: Pool_586_AD002_indexed


title: Pool_584_AD006_indexed


title: Pool_585_AD002_indexed


title: Pool_588_AD002_indexed


title: Pool_585_AD006_indexed


title: Pool_58_AD002_indexed


title: Pool_583_AD006_indexed


title: Pool_582_AD006_indexed


title: Pool_583_AD002_indexed


title: Pool_580_AD002_indexed


title: Pool_581_AD002_indexed


title: Pool_57_AD006_indexed


title: Pool_581_AD006_indexed


title: Pool_579_AD006_indexed


title: Pool_582_AD002_indexed


title: Pool_580_AD006_indexed


title: Pool_57_AD002_indexed


title: Pool_604_AD006_indexed


title: Pool_579_AD002_indexed


title: Pool_578_AD002_indexed


title: Pool_577_AD006_indexed


title: Pool_578_AD006_indexed


title: Pool_576_AD010_indexed


title: Pool_587_AD002_indexed


title: Pool_576_AD008_indexed


title: Pool_575_AD010_indexed


title: Pool_572_AD008_indexed


title: Pool_573_AD010_indexed


title: Pool_574_AD008_indexed


title: Pool_575_AD008_indexed


title: Pool_573_AD008_indexed


title: Pool_572_AD010_indexed


title: Pool_574_AD010_indexed


title: Pool_56_AD006_indexed


title: Pool_571_AD008_indexed


title: Pool_570_AD010_indexed


title: Pool_570_AD008_indexed


title: Pool_565_AD010_indexed


title: Pool_565_AD008_indexed


title: Pool_568_AD010_indexed


title: Pool_566_AD008_indexed


title: Pool_577_AD002_indexed


title: Pool_566_AD010_indexed


title: Pool_567_AD010_indexed


title: Pool_568_AD008_indexed


title: Pool_56_AD002_indexed


title: Pool_567_AD008_indexed


title: Pool_563_AD010_indexed


title: Pool_564_AD010_indexed


title: Pool_562_AD010_indexed


title: Pool_564_AD008_indexed


title: Pool_561_AD010_indexed


title: Pool_562_AD008_indexed


title: Pool_563_AD008_indexed


title: Pool_571_AD010_indexed


title: Pool_561_AD008_indexed


title: Pool_560_AD010_indexed


title: Pool_559_AD010_indexed


title: Pool_557_AD008_indexed


title: Pool_557_AD010_indexed


title: Pool_558_AD008_indexed


title: Pool_558_AD010_indexed


title: Pool_556_AD010_indexed


title: Pool_555_AD010_indexed


title: Pool_556_AD008_indexed


title: Pool_554_AD008_indexed


title: Pool_550_AD008_indexed


title: Pool_552_AD010_indexed


title: Pool_555_AD008_indexed


title: Pool_54_AD006_indexed


title: Pool_550_AD010_indexed


title: Pool_54_AD002_indexed


title: Pool_551_AD010_indexed


title: Pool_549_AD008_indexed


title: Pool_554_AD010_indexed


title: Pool_548_AD008_indexed


title: Pool_552_AD008_indexed


title: Pool_549_AD010_indexed


title: Pool_547_AD010_indexed


title: Pool_55_AD006_indexed


title: Pool_55_AD002_indexed


title: Pool_546_AD010_indexed


title: Pool_560_AD008_indexed


title: Pool_548_AD010_indexed


title: Pool_546_AD008_indexed


title: Pool_545_AD010_indexed


title: Pool_543_AD010_indexed


title: Pool_544_AD008_indexed


title: Pool_542_AD010_indexed


title: Pool_543_AD008_indexed


title: Pool_542_AD008_indexed


title: Pool_541_AD010_indexed


title: Pool_53_AD002_indexed


title: Pool_53_AD006_indexed


title: Pool_538_AD010_indexed


title: Pool_540_AD010_indexed


title: Pool_538_AD008_indexed


title: Pool_537_AD010_indexed


title: Pool_541_AD008_indexed


title: Pool_537_AD008_indexed


title: Pool_534_AD010_indexed


title: Pool_539_AD010_indexed


title: Pool_536_AD008_indexed


title: Pool_534_AD008_indexed


title: Pool_536_AD010_indexed


title: Pool_533_AD010_indexed


title: Pool_532_AD010_indexed


title: Pool_532_AD008_indexed


title: Pool_530_AD008_indexed


title: Pool_544_AD010_indexed


title: Pool_531_AD010_indexed


title: Pool_52_AD006_indexed


title: Pool_529_AD010_indexed


title: Pool_540_AD008_indexed


title: Pool_559_AD008_indexed


title: Pool_528_AD008_indexed


title: Pool_527_AD010_indexed


title: Pool_529_AD008_indexed


title: Pool_535_AD010_indexed


title: Pool_526_AD008_indexed


title: Pool_521_AD010_indexed


title: Pool_51_AD006_indexed


title: Pool_525_AD010_indexed


title: Pool_524_AD008_indexed


title: Pool_524_AD010_indexed


title: Pool_519_AD010_indexed


title: Pool_519_AD008_indexed


title: Pool_526_AD010_indexed