title: HITS-CLIP_N2A

status: Public on Sep 30 2019

type: SRA

city: New York

first: Robert

last: Darnell

state: New York

address: 1230 York Ave

country: USA

institute: Rockefeller University

description:
Elavl-CLIP
Elavl.N2A.CLIP_peaks.txt.gz

library_source: transcriptomic

data_processing:
Library strategy: HITS-CLIP
filtering (min:0-4:20,mean:5-29:20)
collapsing of exact sequences
stripping of 5'degenerate linker (7 or 25nt)
removal of 3'adaptor (GTGTCAGTCACTTCCAGCGG)
alignment with novoalign (unambigous mapping)
collapsing of PCR duplicates
Genome_build: hg19
Supplementary_files_format_and_content: bed file containing genomic coordinates of 50bp region of CLIP peaks

molecule: total RNA

organism: Mus musculus

source_name: N2A cells

growth_protocol: Cells were grown under standard conditions.

extract_protocol:
Brain tissue and cell lines were UV irradiated and subjected to Elavl HITS-CLIP (detailed description in accompanying paper)
partial RNAse digestion, RNA linker ligation and PCR amplificiation
Elavl bound fragments were ligated to a degenerate 5'linker and a 3'linker

tag: tissue

value: cell line

tag: degenerate linker

value: 7nt:begins with GA, ends with G


title: HITS-CLIP_Cortex3

description:
Elavl-CLIP
Elavl.2month.CLIP_peaks.txt.gz

source_name: cortex

value: brain

value: 25nt:begins with GCAT or CATG, ends with G


title: HITS-CLIP_Cortex2

value: 25nt:begins with CGTA or ACCG, ends with G


title: SFO_KO2

city: Gainesville

first: Zongliang

last: Jiang

state: FL

address: 2033 Mowry Rd

department: Animal Sciences

institute: University of Florida

data_processing:
Sequencing adapters were trimmed using Cutadapt and low quality reads were pre-filtered by FASTX-Toolkit before mapping.  The quality of reads after filtering was examined using fastQC.
All filtered reads were aligned to mouse reference genome (GRCm38/mm10) by STAR with default parameters.
Individual mapped reads were quantified to annotation model to calculate gene counts. Differentially expression analysis between Mfn1-/- or WT groups were performed using using DESeq 2 package.
Differentially expressed genes between Mfn1-/- and WT groups were determined using false discovery rate (FDR) of < 0.05, Foldchange > 2 and minimal of 5 reads as cutoffs.
Genome_build: GRCm38/mm10
Supplementary_files_format_and_content: gene counts generated by DESeq2

source_name: Secondary follicle-enclosed oocytes

extract_protocol:
cDNA synthesis and amplification was performed using 5 oocytes per sample and Smart-Seq2 protocol.
Sequencing libraries were constructed using the amplified cDNA and Nextera® XT DNA Library Preparation Kit (Illumina, San Diego, CA) and indexed using Nextera® XT Index Kit (Illumina). Library quality was confirmed using DNA 1000 kit on Tapestation 2200 (Aglilent Technologies), and the libraries were then sequenced on Illumina’s Hiseq 2500 platform with 75bp pair-end reads.

treatment_protocol: Secondary follicles were collected from 8-week-old Mfn1-/- and WT mice by digesting the ovaries with 1.5 mg/mL collagenase type V (Sigma, St. Louis, MO) for 1 h at 37°C in M2 medium (Sigma, St. Louis, MO) [7]. Secondary follicle-enclosed oocytes (referred as the oocytes) and granulosa cells were collected by further digesting the harvested secondary follicle with Accutase solution (Sigma, St. Louis, MO) for 15min.

tag: strain

tag: cell type

value: Secondary follicle-enclosed oocytes

tag: age

tag: genotype


title: SFO_KO1


title: SFO_KO3


title: HITS-CLIP_Cortex1

value: 25nt:begins with GATC or GTGC, ends with G


title: SFO_WT2

value: WT


title: SFO_WT3


title: SFO_WT1


title: GV_KO3

source_name: GV oocytes

extract_protocol:
cDNA synthesis and amplification was performed using 5 oocytes per sample and Smart-Seq2 protocol.
Sequencing libraries were constructed using the amplified cDNA and Nextera® XT DNA Library Preparation Kit (Illumina, San Diego, CA) and indexed using Nextera® XT Index Kit (Illumina). Library quality was confirmed using DNA 1000 kit on Tapestation 2200 (Aglilent Technologies), and the libraries were then sequenced on Illumina Hiseq 2500 with 75bp pair-end reads.

treatment_protocol: GV oocytes were collected from 8-week-old Mfn1-/- and WT mice by digesting the ovaries with 1.5 mg/mL collagenase type V (Sigma, St. Louis, MO) for 1 h at 37°C in M2 medium (Sigma, St. Louis, MO) [7]. Secondary follicle-enclosed oocytes (referred as the oocytes) and granulosa cells were collected by further digesting the harvested secondary follicle with Accutase solution (Sigma, St. Louis, MO) for 15min.

value: GV oocytes


title: GV_KO1


title: GV_KO2


title: SFO_WT4


title: GV_WT2


title: GV_WT1


title: GV_WT3


title: H3K36me3_RA

city: Berlin

first: Laura

middle: V

last: Glaser

address: Ihnestraße 63-73

department: Computational Molecular Biology

country: Germany

institute: Max Planck Institute for Molecular Genetics

library_source: genomic

data_processing:
RNA-seq: Raw sequencing reads were mapped to mm10 reference genome using Burrows-Wheeler Alignment tool (BWA-MEM algorithm, PMID: 19451168), processing fastq to bam files. Mapped reads were sorted using SAMtools (PMID: 19505943) and DESeq2 (PMID: 25516281) was applied for differential gene expression analysis using ENSEMBL gene annotation (Mus_musculus.GRCm38.90).
ChIP-seq: Each ChIP library was sequenced in two separate sequencing runs. Raw sequencing reads were mapped to mm10 reference genome using STAR Alignment tool (STAR_2.5.1b_modified, PMID: 23104886), allowing a maximum intron size of 1 to adjust for non-spliced input data. Subsequently reads from the same library were merged into one file.
ATAC-seq: We processed fastq to bam files using the Burrows-Wheeler Alignment tool (BWA) (PMID: 19451168) for mapping and SAMtools (PMID: 19505943) for filtering, sorting and removing duplicates.
Genome_build: mm10
Supplementary_files_format_and_content: RNA-seq: The DESeq2 derived final data file contains all genes with an adjusted p-value below 0.1 (padj < 0.1).
Supplementary_files_format_and_content: ChIP-seq: Mapped reads were converted to bigwig coverage tracks using the bam2bigwig script (https://github.com/milospjanic/bam2bigwig).
Supplementary_files_format_and_content: ATAC-seq: We called peaks on the alignment results using MACS2 (PMID: 18798982) and converted bam files to bigwig format using the bamToBigWig tool from the bioinformatics library Gonetics (P. Benner. Gonetics. https://github.com/pbenner/gonetics).

molecule: genomic DNA

source_name: Embryonic stem cells

growth_protocol: E14 mouse embryonic stem cells (mESCs) were cultured under feeder-free conditions and routinely passaged every two days in ES medium plus LIF: Glasgow Minimum Essential Medium (Sigma-Aldrich) supplemented with 17% FBS (HycloneTM, SV30160.03, GE Healthcare), 2 mM GlutaMAXTM (Gibco), 100 U/ml Penicillin-Streptomycin (Gibco), 1x MEM Non-Essential Amino Acid Solution (Gibco), 1 mM Sodium Pyruvate (Gibco), 0.5 mM 2-Mercaptoethanol (Gibco), and recombinantly expressed LIF.

extract_protocol:
6x105 low passage (< 10) E14 cells were plated per 15 cm culture dish and cultivated for 48 h in regular ES medium containing LIF. 4 h prior to cross-link cells were treated with LIF or RA as described in the treatment protocol. Then cells were washed with PBS, trypsinized and gently but thoroughly resuspended in ES medium to obtain single cell suspension. Cells were cross-linked adding 1% formaldehyde for 5 min and the reaction was quenched using 125 mM Glycin for another 5 min. After two wash steps with PBS, cell pellets were resuspended in 100 µl sonication buffer (20 mM Tris-HCl pH 7.5, 2 mM EDTA pH 8.0, 1% Triton X-100, 150 mM NaCl, 0.1% SDS and Complete proteinase inhibitor cocktail (Roche)) per 106 cells and chromatin was sheared by sonication applying a Bioruptor Pico (Diagenode) device for 25-35 cycles. Automatic ChIP was performed using the SX-8G Compact IP-Star liquid handler (Diagenode) in combination with Auto Histone Kits (Diagenode, C01010022). Using the pre-programmed method ‘indirect ChIP’, ChIP reactions were carried out in a final volume of 200 μl for 10 h followed by 5 h beads incubation and 5 min washes (at 4°C). After ChIP, eluates were recovered, RNase A-treated, decrosslinked ON at 65°C and treated with Proteinase K for 4 h at 55°C. The recovered DNA was purified using ChIP DNA Clean & Concentrator Kit (Zymo research, D5205).
Sequencing libraries were prepared using the NEBNext® UltraTM DNA Library Prep Kit (NEB, E7370) according to manufacturer’s instructions.

treatment_protocol: E14 mESC cells were cultured in the presence of leukemia inhibitory factor (LIF) in order to maintain the pluripotent state of the cells. To exit from pluripotency and push the cells towards differentiation, LIF was withdrawn and retinoic acid (RA, Sigma, R2625) was added to the medium to a final concentration of 1 µM. For all experiments, 4h prior to harvest, cell culture medium was removed, cells washed twice with PBS and fresh ES medium was added containing either LIF or RA.

tag: cell line

value: E14 ES cells

tag: treatment

value: RA

tag: chip antibody

value: H3K36me3 (Diagenode C15410192)


title: H3K79me2_RA

value: H3K79me2 (Active Motif 39143)


title: H3K122ac_RA

value: H3K122ac (abcam ab33309)


title: Input_RA

description:
dynamicEnh.mESC.txt
clusterEnh.mESCMINUS.bedGraph
singleEnh.mESCMINUS.bedGraph


title: H3K4me3_RA

value: H3K4me3 (Diagenode C15410003)


title: H3K27ac_RA

value: H3K27ac (Diagenode C15410196)


title: H3K4me1_RA

value: H3K4me1 (Diagenode C15410194)


title: H3K27me3_LIF

value: LIF

value: H3K27me3 (Diagenode C15410195)


title: H3K122ac_LIF


title: H3K36me3_LIF


title: H3K27ac_LIF

description:
dynamicEnh.mESC.txt
clusterEnh.mESCPLUS.bedGraph
singleEnh.mESCPLUS.bedGraph


title: H3K4me3_LIF


title: H3K79me2_LIF


title: RNA_RA_rep2

molecule: polyA RNA

extract_protocol:
2x105 low passage (< 10) E14 cells were plated per 10 cm culture dish and cultivated for 48 h in regular ES medium containing LIF. 4 h prior to harvest, medium was exchanged and cells were treated with LIF or RA as described in the treatment protocol. Cells were harvested and subjected to RNA extraction applying RNeasy Mini Kit (Qiagen) according to the manufacturers’ instructions. The experiment was performed in biological triplicates.
Sequencing libraries were generated using TruSeq® Stranded mRNA Kit (Illumina) according to manufacturer’s instructions.


title: RNA_RA_rep3


title: H3K9me3_RA

value: H3K9me3 (Diagenode C15410193)


title: RNA_LIF_rep3


title: RNA_LIF_rep2


title: RNA_RA_rep1


title: RNA_LIF_rep1


title: H3K27me3_RA


title: H3K9me3_LIF


title: BRCA2-52

city: quebec

first: Francine

last: Durocher

state: quebec

address: 2705, Laurier Blvd

department: Molecular Medicine

country: Canada

institute: CHU de Quebec Research Centre

data_processing:
Raw reads were trimmed for length (n>=32), quality (phred33 score >= 30) and adaptor sequence using fastxv0.0.13.1.
Trimmed paired-end reads (read length: 100 bp) were aligned to the hg19 human reference genome using Tophat version v1.4.0 (tarpnel et al. 2009)
Raw, trimmed and aligned read numbers were retrieved after alignment to determine the quality of the sequence data. GATK (v1.0.5777) (DePristo et al. 2009) was then used to compute the coding sequence coverage for each sample. Raw read counts and normalized read counts (in transcript per million, TPM) were obtained using the Kallisto v0.43.0 quant command (Bray et al. 2015) with the default params on the GRCh38.rel79 version of the human transcriptome, while Pearson correlation values were obtained pairwise for each sample using R v2.12.0. Differential gene expression was determined using edgeRv2.2.6 (Robinson et al. 2010) on R v2.12.0 and DESeqv1.6.1 (Anders et al. 2010) on R v2.14.0. Transcript differential expression was performed using cuffdiff v1.3.0. A gene ontology analysis was then launched on gene and transcript differential expression results using goseqv1.2.1 (Young et al. 2010) on R v2.12.0. Finally, UCSC compatible wiggle tracks were generated using FindPeaks v4.0.16.
Genome_build: hg19 human reference genome
Supplementary_files_format_and_content: excel file including tpm values for each transcript and each sample

organism: Homo sapiens

source_name: lymphoblastoid cell line

growth_protocol: RPMI-1640 (Invitrogen, catalog no.21870076) medium was used for cell culture in this report, supplemented with 15% FBS (wisent, catalog no.80150) for the first month with the addition of cyclosporine A 1.5ug/ml and then followed with 10% FBS. The two culture media were supplemented with glutamine 2mM (wisent, catalog no.25005CI),  penicillin/streptomycin 1unit/ml (wisent, catalog no.30002CI) and fungizone 2.5mg/ml (Squibb canada, catalog no.DIN 00029149).

extract_protocol:
Total RNA was extracted from LCLs using TRI Reagent (Molecular Reasearch Center Inc, Cincinnati, OH, USA) according to manufacturer’s instructions
Illumina RNA seq kit was used for sequence library preparation based on the Illumina TruSeq RNA Sample Preparation protocol.

treatment_protocol: The B lymphocytes were isolated by Ficoll-Paque-plus gradient (Amersham, catalog no.17716700). The total number of lymphocytes was determining using an hemacytometer. Immortalization was made with Eipstein-barr virus (EBV).

value: Immortalized lymphoblastoid cell line

tag: status of disease

value: unaffected

tag: group

tag: mutation


title: BRCA2-1

value: affected


title: BRCA2-53


title: BRCA2-51


title: BRCA2-2


title: BRCA2-3


title: H3K4me1_LIF


title: Input_LIF


title: BRCA2-49


title: BRCA2-46


title: BRCA2-42


title: BRCA2-38


title: BRCA2-47


title: BRCA2-34


title: BRCA2-37


title: BRCA2-41


title: BRCA2-35


title: BRCA2-28


title: BRCA2-15


title: BRCA2-31


title: BRCA2-24


title: BRCA2-55


title: BRCA2-50


title: BRCA2-54


title: BRCA2-14


title: BRCA2-48


title: BRCA2-45


title: BRCA2-43


title: BRCA2-56


title: BRCA2-29


title: BRCA2-36


title: BRCA2-32


title: BRCA2-40


title: BRCA2-30


title: BRCA2-44


title: BRCA2-27


title: BRCA2-39


title: BRCA2-33


title: BRCA2-26


title: BRCA2-23


title: BRCA2-20


title: BRCA2-21


title: BRCA2-19


title: BRCA2-25


title: BRCA2-18


title: BRCA2-16


title: BRCA2-13


title: BRCA2-17


title: BRCA2-11


title: BRCA2-12


title: BRCA2-22


title: BRCA1-1


title: BRCA1-3


title: BRCA1-42


title: BRCA1-38


title: BRCA1-37


title: BRCA1-2


title: BRCA1-35


title: BRCA1-22


title: BRCA1-30


title: BRCA1-13


title: BRCA1-25


title: BRCA1-26


title: BRCA1-11


title: BRCA1-41


title: BRCA1-18


title: BRCA1-43


title: BRCA1-40


title: BRCA1-39


title: BRCA1-36


title: BRCA1-34


title: BRCA1-33


title: BRCA1-31


title: BRCA1-29


title: BRCA1-27


title: BRCA2-10


title: BRCA1-32


title: BRCA1-28


title: BRCA1-24


title: BRCA1-19


title: BRCA1-20


title: BRCA1-21


title: BRCA1-17


title: BRCA1-23


title: BRCA1-15


title: BRCA1-12


title: BRCA1-14


title: BRCA1-16


title: BRCAX-3

value: BRCAX


title: BRCA1-10


title: BRCAX-5


title: BRCAX-2


title: BRCAX-1


title: BRCAX-4


title: BRCAX-6


title: BRCAX-44


title: BRCAX-23


title: BRCAX-42


title: BRCAX-21


title: BRCAX-24


title: BRCAX-43


title: BRCAX-41


title: BRCAX-40


title: BRCAX-19


title: BRCAX-38


title: BRCAX-18


title: BRCAX-20


title: BRCAX-39


title: BRCAX-37


title: BRCAX-15


title: BRCAX-36


title: BRCAX-17


title: BRCAX-34


title: BRCAX-14


title: BRCAX-16


title: BRCAX-35


title: BRCAX-33


title: BRCAX-12


title: BRCAX-11


title: BRCAX-22


title: BRCAX-31


title: BRCAX-13


title: BRCAX-32


title: hep1_A2

city: Tulln/Donau

first: Stefan

last: Boedi

address: Konrad Lorenzstr. 24

country: Austria

institute: BOKU

data_processing:
To analyse the transcriptomes within pathogenic and saprophytic Fusarium graminearum-Triticum aestivum interactions, as well as axenic F. graminearum cultivations, the RNA-seq reads were mapped on the reference genome of Fusarium graminearum or Triticum aestivum using TopHat (v2.0.8) (Trapnell et al., 2012). The interval for allowed intron length was set to a minimum of 20 nt and maximum of 1 kb (Trapnell et al., 2009, Kim et al., 2013).
We used Cufflinks to determine the abundance of transcripts in FPKM (Fragments Per Kilobase of exon per Million fragments mapped) and calculated differentially expressed genes using Cuffdiff (Trapnell et al., 2010, Trapnell et al., 2012). The gene models were included as raw junctions. Transcripts with a FPKM count of zero in one condition were set to the minimum global FPKM count of both conditions.
Genome_build: The reference genome for mapping the F. graminearum reads was retrieved from Wong et al. 2011 (PMID:21051345). To map the T. aestivum reads we used the reference genome given by Mayer et al. 2014 (PMID:25035500).
Supplementary_files_format_and_content: GSE96705_hep1_raw_fpkm.txt
Supplementary_files_format_and_content: GSE96705_wheat_raw_fpkm.txt (abundance data derived from "mixed read" files; see file headers for GEO Sample numbers).

organism: Fusarium graminearum

source_name: hep1 axenic growth

growth_protocol: The highly susceptible cultivar Remus (pedigree: Sappo/Mex//Famos) (Buerstmayr et al., 2002) was used in this study. On average 20 spikelets per wheat head were inoculated by pipetting 20 000 macroconidia (10 µL of a 2* 10^6 spores/ mL suspension) on the reproductive part between the lemma and palea of the two basal florets during anthesis (resulting in 8*10^5 spores per wheat head). Mock ears were inoculated the same way, but using water instead of spore suspension. Three ears were inoculated on the living plant representing pathogenic growth of the fungus and 3 ears, which were cut off the plant and shock-frozen in liquid N prior to spore application, were inoculated as “non- response” control from the wheat side or, in other words, representing saprophytic growth of the fungus. After inoculation the wheat heads on the living plants were covered with moistened plastic bags for the first 24 hours to provide high humidity. The inoculated dead wheat heads were placed in glass petri dishes (Ø 140 mm; h 20 mm). Incubation conditions were set at 20°C, 50% relative humidity during daytime and 18°C, 50% humidity during night with a 16 h photoperiod. Harvesting was performed 3 dai and 5 dai by freezing the plant material in liquid nitrogen. Only palea and lemma of the inoculated florets were sampled including the respective part of the rachis. Additionally completely untreated wheat heads were sampled.

extract_protocol:
RNA isolation was performed using RNeasy Plant Mini Kit (Qiagen, 74904) following the instructions of the manufacturer with one modification and one extension: Instead of eluting RNA once in 30 µL RNase- free water, elution was performed in 2x 20 µL RNase- free water with a 3 minute standing period at room temperature after each water application to the column. Additionally on-column DNase digestion was done during the extraction protocol by using RNase-Free DNase Set (Qiagen, 79254). RNA quantity was first checked on the NanoDrop 2000c Spectrophotometer (Thermo Scientific). Afterwards RNA quantity and integrity was validated by using the Agilent RNA 6000 Nano Kit (Agilent Technologies, 5067-1511) on an Agilent 2100 Bioanalyzer machine following the instructions of the provider. The cDNA Synthesis was done with the RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Scientific, # K1632) using random hexamer primer and following the manufacturer´s protocol.
Illumina sequencing libraries were made from RNA samples according to TruSeq RNA Sample prep kit v2 (Illumina) following the manufacturers protocol with 1 µg total RNA input. 50 bp single end sequencing was performed using a HiSeq Illumina sequencer. Obtained sequences were de-multiplexed, quality controlled and mapped on the Fusarium graminearum PH-1 genome assembly (p3_p13839_Fus_grami_v32).

tag: medium

value: Minimal medium

tag: host status


title: hep1_A1


title: hep1_S3

source_name: hep1 saprophytic growth

value: Wheat

value: dead


title: hep1_S2


title: hep1_P3

source_name: hep1 pathogenic growth

value: alive


title: hep1_S1


title: Combinatorial_library_cells_rep1

city: Cleveland

first: Kenneth

last: Matreyek

state: OH

address: 2103 Cornell Rd

department: Pathology

institute: Case Western Reserve University

description:
Targeted barcode amplification from DNA
Enrichment_scores.csv
e1

data_processing:
Libraries were sequenced on NextSeq 500 instruments (Illumina) and base called using the instruments' Real Time Analysis software.
Sequencing reads were converted to fastq format and de-multiplexed with bcl2fastq. R1 and R2 were trimmed to the first 16 nucleotides,which includes both the eight nucleotides of known sequence serving as a transgene identifier, as well as the flanking four degenerate nucleotides serving as unique plasmid identifier sequences. These datafiles were used to identify each unique plasmid molecule which had been recombined. In subsequent analyses only looking at the eight nucleotides serving as a transgene identifier, the initial fastq files were trimmed such that only nucleotides 5-12 remained. Both steps were performed using custom python scripts which are posted on the lab Github page.
The properly trimmed fastq files were concatenated to create a single sequence denoting the paired transgene combination. The resulting fastq file was entered into Enrich2, and Log2 enrichment scores for each transgene combination was calculated for each replicate experiment. Scores from replicate experiments were averaged to obtain a single average enrichment score, as well as its standard error.
Genome_build: NONE
Supplementary_files_format_and_content: The "Combination_indices.csv" files lists the indices that denote which transgene is present based on the Illumina sequencing data. The "Enrichment_scores.csv" file is the final result of running the Illumina fastq files through trimming (described above) for the indices, Enrich, and associating the enrichment scores for each index combination back to the transgenes they represent. In this file, the number following "e" represents the replicate number. Columns with the letter "s" following the number are columns containing the enrichment score for that replicate. Columns with the letter "e" contain the standard error for that replicate.

source_name: Human-derived cultured cell line

growth_protocol: HEK 293T LLP-rEF1alpha cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum, 100 U/mL penicillin, 0.1 mg/mL streptomycin

extract_protocol:
Cells were transferred into a microfuge tube, pelleted and stored at -20˚C. Genomic DNA was prepared using the GentraPrep kit (Qiagen) or Dneasy kit (Qiagen).
40 ng of plasmid DNA was mixed with 0.333 µM of forward primer, 0.333 µM of reverse primer, and 2x Kapa Hifi added as half the volume (Supplementary table 1). The mixtures were cycled 12 times in a thermocycler. 1 µl of DPNI enzyme (20 units) was added to each tube, and incubated at 37◦C for 2 hours. 15 µl of product was mixed with 3 µl of (6x gel loading dye), and run on a 0.8 percent agarose gel in TBE. Bands were cut, position 1 bands and position 2 bands were pooled separately, and extracted using the Qiagen Gel Extraction kit. Position 1 and position 2 DNA were eluted in 10 µl nuclease-free water. 5 µl of each eluate were mixed together with 10 µl 2x Gibson mix and incubated at 50◦C for 30 minutes. After incubation, the product was eluted in 5 µl, and 4 µl of library was electroporated into 10-beta cells (NEB) using 0.1 µm cuvettes (Bio-Rad) according to manufacturer instructions. Approximately 2.16 million colony forming units of transformants were grown in 200 ml Luria Broth overnight, and plasmid DNA was extracted with a Qiagen Midiprep Kit (Qiagen). 320 ng of library DNA was mixed with 2x Kapa Hifi Readymix and a final concentration of 0.333 µM KAM2311 and 0.333 µM KAM2312, with 30 µl distributed into 8 replicate tubes. 160 ng of rIP2M_attB_GFP plasmid was mixed with 2x Kapa Hifi and a final concentration of 0.333 µM KAM2313 and 0.333 µM KAM2314, with 30 µl distributed into 4 replicate tubes. Both sets of tubes were amplified over 18 cycles. 1 µl (20 units) of DPNI enzyme was added to each tube, and incubated at 37◦C for 2 hours. After incubation, the rIP2M_attB_GFP amplicon was gel extracted from a 0.8% agarose gel and eluted in 10 µl, whereas the library amplicon was cleaned and concentrated (Zymo), and eluted in 5 µl. 4 µl of each were mixed together with 8 µl of 2x Gibson mix and incubated at 50◦C for 30 minutes. The product was clean-and-concentrated, eluted in 5 µl, and electroporated in 10-beta cells using 0.1 µm cuvettes (Bio-Rad) according to manufacturer's instructions. Approximately 0.5 million colony forming units were amplified in 200 ml Luria Broth overnight, and extracted using the Qiagen Midiprep kit. Sanger sequencing of 8 colonies revealed 6 to be intended library members. This library was called attB-TSPCR.

treatment_protocol: Cells harboring variant libraries, prepared as described above, were grown in the presence of 2 μg/mL doxycycline, incuding expression of the variant library. Cells were passaged over approximately two weeks, with a subset of cells collected and frozen for genomic extraction and sequencing after each replicate was complete.

value: HEK 293T LLP-rEF1alpha


title: Control0uM16hrD

city: Lexington

first: Hualin

last: Xi

state: Massachusetts

address: 34 webb st

institute: AbbVie

data_processing:
aligned to hg19 reference genome using STAR v2.4
count uniquely aligned reads per gene using FeatureCount from Subread package and gencode v19 as the gene model annotation
Supplementary_files_format_and_content: read counts for each gene in tab delimited format

growth_protocol: HepG2 cells (ATCC) were cultured in growth media (DMEM supplemented with 1% Penn/Strep, 1% MEM, 1% Glutamax, and 10% Serum) at 37C, 5% CO2 and 85% relative humidity. For treatments, 10^5 cells in 1 ml growth media were seeded into 24-well culture dishes and allowed to settle for 6 hours.

extract_protocol:
Cells were then stripped of media, washed once with PBS, and were lysed by the addition of 1 ml Trizol. Total RNA was then isolated by Trizol-chloroform extraction, followed by RNeasy cleanup.
3'Pool-seq

treatment_protocol: Growth media was replaced with serum-free media containing the indicated concentrations of troglitazone or pioglitazone (Sigma-Aldrich) and vehicle (DMSO, 0.1% final concentration), and the treated cells were allowed to grow for 2 or 16 hours.

value: HepG2 cells

tag: passage

value: ATCC HB-8065

value: untreated, 16hr


title: Veh0uM16hrD


title: Pio1uM16hrD

value: 1uM Pio, 16hr


title: Combinatorial_library_cells_rep6

description:
Targeted barcode amplification from DNA
Enrichment_scores.csv
e6


title: Combinatorial_library_cells_rep4

description:
Targeted barcode amplification from DNA
Enrichment_scores.csv
e4


title: Combinatorial_library_cells_rep3

description:
Targeted barcode amplification from DNA
Enrichment_scores.csv
e3


title: Combinatorial_library_cells_rep7

description:
Targeted barcode amplification from DNA
Enrichment_scores.csv
e7


title: Combinatorial_library_cells_rep2

description:
Targeted barcode amplification from DNA
Enrichment_scores.csv
e2


title: Trog25uM16hrD

value: 25uM Trog, 16hr


title: Trog5uM16hrD

value: 5uM Trog, 16hr


title: Pio25uM16hrD

value: 25uM Pio, 16hr


title: Veh0uM16hrC


title: Pio0.2uM16hrC

value: 0.2uM Pio, 16hr


title: hep1_P2


title: hep1_P1


title: Control0uM16hrC


title: Pio5uM16hrC

value: 5uM Pio, 16hr


title: Combinatorial_library_cells_rep8

description:
Targeted barcode amplification from DNA
Enrichment_scores.csv
e8


title: Combinatorial_library_cells_rep5

description:
Targeted barcode amplification from DNA
Enrichment_scores.csv
e5


title: Pio25uM16hrC


title: Trog1uM16hrC

value: 1uM Trog, 16hr


title: Trog25uM16hrC


title: Pio5uM16hrD


title: Control0uM16hrB


title: Trog0.2uM16hrD

value: 0.2uM Trog, 16hr


title: Veh0uM16hrB


title: Trog1uM16hrD


title: Pio0.2uM16hrB


title: Pio0.2uM16hrD


title: Pio25uM16hrB


title: Pio5uM16hrB


title: Pio1uM16hrC


title: Control0uM16hrA


title: Trog5uM16hrB


title: Trog25uM16hrB


title: Pio0.2uM16hrA


title: Veh0uM16hrA


title: Pio5uM16hrA


title: Trog5uM16hrC


title: Pio25uM16hrA


title: Trog0.2uM16hrA


title: Trog5uM16hrA


title: Trog25uM16hrA


title: Control0uM2hrD

value: untreated, 2hr


title: Pio0.2uM2hrD

value: 0.2uM Pio, 2hr


title: Trog0.2uM16hrC


title: Pio1uM2hrD

value: 1uM Pio, 2hr


title: Trog1uM16hrB


title: Trog25uM2hrD

value: 25uM Trog, 2hr


title: Pio25uM2hrD

value: 25uM Pio, 2hr


title: Trog1uM2hrD

value: 1uM Trog, 2hr


title: Pio1uM16hrB

value: HepG2 cells


title: Veh0uM2hrC

value: ATCC HB-8065

value: 0.1% DMSO, 2hr


title: Pio1uM16hrA

value: 1uM Pio, 16hr


title: Trog0.2uM16hrB


title: Pio0.2uM2hrC


title: Pio25uM2hrC


title: Trog1uM2hrC


title: Trog5uM2hrC

value: 5uM Trog, 2hr


title: Control0uM2hrC


title: Pio5uM2hrC

value: 5uM Pio, 2hr


title: Trog1uM16hrA


title: Veh0uM2hrB


title: Trog25uM2hrC


title: Veh0uM2hrD


title: Control0uM2hrB


title: Pio0.2uM2hrB


title: Pio1uM2hrB


title: Pio5uM2hrD


title: Pio25uM2hrB


title: Trog0.2uM2hrB

value: 0.2uM Trog, 2hr


title: Trog0.2uM2hrD


title: Trog1uM2hrB


title: Trog5uM2hrD


title: Trog25uM2hrB


title: Trog5uM2hrB


title: Pio1uM2hrC


title: Control0uM2hrA


title: Veh0uM2hrA

value: 0.1% DMSO Control, 2hr


title: Pio0.2uM2hrA


title: Trog0.2uM2hrC


title: Pio5uM2hrA


title: Pio25uM2hrA


title: Trog0.2uM2hrA


title: Trog25uM2hrA


title: Trog1uM2hrA


title: Patient_T6 [4Bb]

city: Newcastle upon Tyne

first: Andrew

middle: James

last: Skelton

state: Tyne and Wear

address: Framlington Place

department: Bioinformatics

country: United Kingdom

institute: Newcastle University

description:
Patient primary dermal fibroblast, T6
4Bb

data_processing:
Transcript Level Quantification Using Salmon 0.9.1
Aggregated gene level quantification using DESeq2
Library Scaled Normalisation using DESeq2
Genome_build: GRCh38 (Gencode v24)
Supplementary_files_format_and_content: Gene Level Counts, Tab Delimited Text (Raw, Normalised)

source_name: primary dermal fibroblast

growth_protocol: DMEM with 10% FBS, 1% penicillin-streptomycin

extract_protocol:
Extracted with ReliaPrep RNA isolation kit according to manufacturers protocol
Truseq stranded mRNA library preparation

treatment_protocol: Recombinant human IFN-alpha (1000 IU/mL) or media only (mock)

tag: timepoint(hrs)

tag: pair

tag: sampletype

value: Patient


title: Patient_T12 [4Cb]

description:
Patient primary dermal fibroblast, T12
4Cb


title: Patient_T0 [4Ab]

description:
Patient primary dermal fibroblast, T0
4Ab


title: Patient_T0 [4Ac]

description:
Patient primary dermal fibroblast, T0
4Ac


title: Patient_T12 [4Ca]

description:
Patient primary dermal fibroblast, T12
4Ca


title: Patient_T6 [4Bc]

description:
Patient primary dermal fibroblast, T6
4Bc


title: Patient_T0 [4Aa]

description:
Patient primary dermal fibroblast, T0
4Aa


title: Patient_T6 [4Ba]

description:
Patient primary dermal fibroblast, T6
4Ba


title: Control_T12 [3Ca]

description:
Control primary dermal fibroblast, T12
3Ca

value: Control


title: Control_T6 [3Bb]

description:
Control primary dermal fibroblast, T6
3Bb


title: Control_T6 [3Bc]

description:
Control primary dermal fibroblast, T6
3Bc


title: Control_T6 [3Ba]

description:
Control primary dermal fibroblast, T6
3Ba


title: Control_T12 [3Cc]

description:
Control primary dermal fibroblast, T12
3Cc


title: Control_T12 [3Cb]

description:
Control primary dermal fibroblast, T12
3Cb


title: Pio1uM2hrA

tag: tissue


title: Control_T0 [3Ac]

description:
Control primary dermal fibroblast, T0
3Ac


title: Control_T12 [2Cb]

description:
Control primary dermal fibroblast, T12
2Cb


title: Control_T12 [2Ca]

description:
Control primary dermal fibroblast, T12
2Ca


title: Control_T12 [2Cc]

description:
Control primary dermal fibroblast, T12
2Cc


title: Control_T0 [3Aa]

description:
Control primary dermal fibroblast, T0
3Aa


title: Control_T6 [2Bc]

description:
Control primary dermal fibroblast, T6
2Bc


title: Control_T6 [2Bb]

description:
Control primary dermal fibroblast, T6
2Bb


title: Trog5uM2hrA


title: Control_T0 [3Ab]

description:
Control primary dermal fibroblast, T0
3Ab


title: Control_T6 [2Ba]

description:
Control primary dermal fibroblast, T6
2Ba


title: Control_T0 [2Ac]

description:
Control primary dermal fibroblast, T0
2Ac


title: Control_T0 [2Ab]

description:
Control primary dermal fibroblast, T0
2Ab


title: Control_T0 [2Aa]

description:
Control primary dermal fibroblast, T0
2Aa


title: Patient_T12 [4Cc]

description:
Patient primary dermal fibroblast, T12
4Cc


title: Control_T12 [1Cb]

description:
Control primary dermal fibroblast, T12
1Cb


title: Control_T12 [1Ca]

description:
Control primary dermal fibroblast, T12
1Ca


title: Control_T6 [1Bc]

description:
Control primary dermal fibroblast, T6
1Bc


title: Control_T6 [1Ba]

description:
Control primary dermal fibroblast, T6
1Ba


title: Control_T6 [1Bb]

description:
Control primary dermal fibroblast, T6
1Bb


title: Control_T0 [1Ac]

description:
Control primary dermal fibroblast, T0
1Ac


title: Control_T0 [1Ab]

description:
Control primary dermal fibroblast, T0
1Ab


title: Control_T12 [1Cc]

description:
Control primary dermal fibroblast, T12
1Cc


title: IRF1 KO  IFN Beta treated at 24hr rep 3

city: Minneapolis

first: Michael

last: Gale

state: Minnesota

address: 689 23rd Ave SE

department: Microbiology and Immunology

institute: University of Minnesota

description:
IRF1 KO  IFN Beta treated at 24hours replicate number  3
Column header name in count_matrix.txt:
036_SG_Savan_02_A24_Beta_KO_24hr_RNA_mRNA_run02_S12

data_processing:
Constructed libraries were sequenced on an NextSeq 500 Illumina platform, producing 2x75nt stranded paired end reads (52 Gb). Image analysis, base calling, and error estimation were performed using Illumina Analysis Pipeline (version 2.8).
Quality control of the primary sequencing data was performed using FastQC (version 0.11.3) and included adapter trimming using cutadapt (version 1.8.3).
Reads were aligned using STAR (version 2.4.0)
We mapped reads to the human reference genome (GRCh37) from Illumina's igenomes using STAR. After mapping, we assigned aligned read counts from BAM files to exons and genes using the python package HT-Seq.  HT-Seq provided the most accurate way of aligning read counts to overlapping exons. Reads that mapped to multiple positions were removed.
Genome_build: Human genome (ENSEMBL, GRCh37)
Supplementary_files_format_and_content: count_matrix.txt reports raw counts

source_name: IRF1 KO  IFN Beta treated at 24hr

growth_protocol: Immortalized human hepatocytes PH5CH8 were cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum (FBS), 2mM Glutamine, 100 U/ml Penicillin and 100 µg/ml Streptomycin and maintained at 37°C in 5% CO2.

extract_protocol:
Total RNA was extracted using RNeasy mini kit (QIAGEN) with on-column DNase treatment.
KAPA stranded RNA-seq kit with Ribo Erase
Library quality was evaluated using the Qubit® 3.0 Fluorometer and the Agilent 2100 Bioanalyzer instrument. Constructed libraries were sequenced on an NextSeq 500 Illumina platform, producing 2x75nt stranded paired end reads (52 Gb). Image analysis, base calling, and error estimation were performed using Illumina Analysis Pipeline (version 2.8).

treatment_protocol: PH5CH8 control and IRF1 KO cells were stimulated with 25 IU/ml IFNβ or 100 ng/ml of IFNλ3 for 12 or 24h (n=3 per treatment). Genotype-matched untreated cells were used as mock controls (n=3 per genotype).

value: immortalized human hepatocytes PH5CH8 cells

tag: time

value: Beta

value: IRF1 KO

tag: replicate


title: IRF1 KO  IFN Beta treated at 12hr rep 3

description:
IRF1 KO  IFN Beta treated at 12hours replicate number  3
Column header name in count_matrix.txt:
033_SG_Savan_02_A21_Beta_KO_12hr_RNA_mRNA_run03_S11

source_name: IRF1 KO  IFN Beta treated at 12hr


title: IRF1 KO  IFN Beta treated at 24hr rep 1

description:
IRF1 KO  IFN Beta treated at 24hours replicate number  1
Column header name in count_matrix.txt:
034_SG_Savan_02_A22_Beta_KO_24hr_RNA_mRNA_run02_S11


title: IRF1 KO  IFN Beta treated at 24hr rep 2

description:
IRF1 KO  IFN Beta treated at 24hours replicate number  2
Column header name in count_matrix.txt:
035_SG_Savan_02_A23_Beta_KO_24hr_RNA_mRNA_run03_S12


title: PH5CH8 control  IFN Lambda treated at 24hr rep 3

description:
PH5CH8 control  IFN Lambda treated at 24hours replicate number  3
Column header name in count_matrix.txt:
027_SG_Savan_02_A15_Lambda_CTRL_24hr_RNA_mRNA_run03_S9

value: Lambda

value: CTRL


title: IRF1 KO  mock treated at 12hr rep 2

description:
IRF1 KO  mock treated at 12hours replicate number  2
Column header name in count_matrix.txt:
029_SG_Savan_02_A17_mock_KO_12hr_RNA_mRNA_run02_S10

source_name: IRF1 KO  mock treated at 12hr

value: mock


title: IRF1 KO  mock treated at 12hr rep 3

description:
IRF1 KO  mock treated at 12hours replicate number  3
Column header name in count_matrix.txt:
030_SG_Savan_02_A18_mock_KO_12hr_RNA_mRNA_run01_S11


title: IRF1 KO  IFN Beta treated at 12hr rep 2

description:
IRF1 KO  IFN Beta treated at 12hours replicate number  2
Column header name in count_matrix.txt:
032_SG_Savan_02_A20_Beta_KO_12hr_RNA_mRNA_run01_S12


title: PH5CH8 control  IFN Lambda treated at 24hr rep 2

description:
PH5CH8 control  IFN Lambda treated at 24hours replicate number  2
Column header name in count_matrix.txt:
026_SG_Savan_02_A14_Lambda_CTRL_24hr_RNA_mRNA_run01_S9


title: PH5CH8 control  IFN Beta treated at 24hr rep 1

description:
PH5CH8 control  IFN Beta treated at 24hours replicate number  1
Column header name in count_matrix.txt:
022_SG_Savan_02_A10_Beta_CTRL_24hr_RNA_mRNA_run02_S8

source_name: PH5CH8 control  IFN Beta treated at 24hr


title: PH5CH8 control  IFN Lambda treated at 12hr rep 3

description:
PH5CH8 control  IFN Lambda treated at 12hours replicate number  3
Column header name in count_matrix.txt:
021_SG_Savan_02_A9_Lambda_CTRL_12hr_RNA_mRNA_run01_S7


title: IRF1 KO  mock treated at 12hr rep 1

description:
IRF1 KO  mock treated at 12hours replicate number  1
Column header name in count_matrix.txt:
028_SG_Savan_02_A16_mock_KO_12hr_RNA_mRNA_run01_S10


title: PH5CH8 control  IFN Lambda treated at 24hr rep 1

description:
PH5CH8 control  IFN Lambda treated at 24hours replicate number  1
Column header name in count_matrix.txt:
025_SG_Savan_02_A13_Lambda_CTRL_24hr_RNA_mRNA_run02_S9


title: PH5CH8 control  IFN Beta treated at 24hr rep 3

description:
PH5CH8 control  IFN Beta treated at 24hours replicate number  3
Column header name in count_matrix.txt:
024_SG_Savan_02_A12_Beta_CTRL_24hr_RNA_mRNA_run01_S8


title: PH5CH8 control  IFN Beta treated at 12hr rep 3

description:
PH5CH8 control  IFN Beta treated at 12hours replicate number  3
Column header name in count_matrix.txt:
018_SG_Savan_02_A6_Beta_CTRL_12hr_RNA_mRNA_run03_S6

source_name: PH5CH8 control  IFN Beta treated at 12hr


title: PH5CH8 control  IFN Beta treated at 24hr rep 2

description:
PH5CH8 control  IFN Beta treated at 24hours replicate number  2
Column header name in count_matrix.txt:
023_SG_Savan_02_A11_Beta_CTRL_24hr_RNA_mRNA_run03_S8


title: PH5CH8 control  IFN Beta treated at 12hr rep 2

description:
PH5CH8 control  IFN Beta treated at 12hours replicate number  2
Column header name in count_matrix.txt:
017_SG_Savan_02_A5_Beta_CTRL_12hr_RNA_mRNA_run02_S7


title: PH5CH8 control  IFN Lambda treated at 12hr rep 1

description:
PH5CH8 control  IFN Lambda treated at 12hours replicate number  1
Column header name in count_matrix.txt:
019_SG_Savan_02_A7_Lambda_CTRL_12hr_RNA_mRNA_run01_S6


title: PH5CH8 control  IFN Lambda treated at 12hr rep 2

description:
PH5CH8 control  IFN Lambda treated at 12hours replicate number  2
Column header name in count_matrix.txt:
020_SG_Savan_02_A8_Lambda_CTRL_12hr_RNA_mRNA_run03_S7


title: PH5CH8 control  mock treated at 12hr rep 3

description:
PH5CH8 control  mock treated at 12hours replicate number  3
Column header name in count_matrix.txt:
015_SG_Savan_02_A3_mock_CTRL_12hr_RNA_mRNA_run02_S6

source_name: PH5CH8 control  mock treated at 12hr


title: PH5CH8 control  mock treated at 12hr rep 1

description:
PH5CH8 control  mock treated at 12hours replicate number  1
Column header name in count_matrix.txt:
013_SG_Savan_02_A1_mock_CTRL_12hr_RNA_mRNA_run02_S5


title: PH5CH8 control  IFN Beta treated at 12hr rep 1

description:
PH5CH8 control  IFN Beta treated at 12hours replicate number  1
Column header name in count_matrix.txt:
016_SG_Savan_02_A4_Beta_CTRL_12hr_RNA_mRNA_run03_S5


title: PH5CH8 control  mock treated at 12hr rep 2

description:
PH5CH8 control  mock treated at 12hours replicate number  2
Column header name in count_matrix.txt:
014_SG_Savan_02_A2_mock_CTRL_12hr_RNA_mRNA_run01_S5


title: IRF1 KO  IFN Beta treated at 12hr rep 1

description:
IRF1 KO  IFN Beta treated at 12hours replicate number  1
Column header name in count_matrix.txt:
031_SG_Savan_02_A19_Beta_KO_12hr_RNA_mRNA_run03_S10


title: CJ4_30_T_rep_4

city: Macclesfield

first: Claus

last: Jorgensen

state: Lancs

address: Alderley Park

institute: Cancer Research UK Manchester Institute

data_processing:
NB Dataset consists of two RNA sequencing runs, each containing 4 lanes. Each sample was sequenced within each lane of each run i.e. 8 times in total.
Sequencing reads were aligned to the mouse reference genome using the BCBio-NextGen RNA-seq pipeline using the STAR aligner.
Gene annotation was taken from Ensembl build 83. Read counts were determined by using the featureCounts function from the Bioconductor package Rsubread (version 1.16.1).
Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using the EdgeR package.
Genome_build: mm10
Supplementary_files_format_and_content: CSV files contain aligned read counts per sample, and normalised RPKM values.

source_name: Murine pancreatic fibroblasts_Co-cultured with Clone T

growth_protocol: Tumour sub-clones and fibroblasts were co-cultured for 48hours before resolving according to tumour cell-specific GFP expression by FACS

extract_protocol:
Isolated fibroblasts were pelleted and lysed in QIAgen RLT buffer. RNA was extracted using a Qiagen RNeasy plus kit.
Indexed PolyA libraries were prepared using 200ng of total RNA and 14 cycles of amplification with the Agilent SureSelect Strand Specific RNA Library Prep Kit for Illumina Sequencing.
Paired-end 76 base-pair sequencing was carried out by clustering 2pM of the pooled libraries on a Illumina NextSeq 500 sequencer

tag: strain

tag: experimental condition

value: Co-cultured with Clone T

tag: cell type

value: Murine pancreatic fibroblasts


title: CJ4_06_T_rep_1


title: CJ4_22_T_rep_3


title: CJ4_14_T_rep_2


title: CJ4_23_V_rep_3

source_name: Murine pancreatic fibroblasts_Co-cultured with Clone V

value: Co-cultured with Clone V


title: CJ4_36_PSC_rep_4

value: Mono-culture


title: CJ4_15_V_rep_2


title: CJ4_31_V_rep_4


title: CJ4_33_PSC_rep_1


title: CJ4_07_V_rep_1


title: CJ4_34_PSC_rep_2


title: CJ4_21_N_rep_3

source_name: Murine pancreatic fibroblasts_Co-cultured with clone N

value: Co-cultured with clone N


title: CJ4_13_N_rep_2


title: CJ4_04_M_rep_1

source_name: Murine pancreatic fibroblasts_Co-cultured with clone M

value: Co-cultured with clone M


title: CJ4_12_M_rep_2


title: CJ4_05_N_rep_1


title: CJ4_35_PSC_rep_3


title: CJ4_32_KPC1_rep_4

source_name: Murine pancreatic fibroblasts_Co-cultured with KPC1

value: Co-cultured with KPC1


title: CJ4_28_M_rep_4


title: CJ4_29_N_rep_4


title: CJ4_20_M_rep_3


title: CJ4_16_KPC1_rep_2


title: CJ4_27_K_rep_4

source_name: Murine pancreatic fibroblasts_Co-cultured with Clone K

value: Co-cultured with Clone K


title: CJ4_11_K_rep_2


title: CJ4_08_KPC1_rep_1


title: CJ4_19_K_rep_3


title: CJ4_03_K_rep_1


title: Pio5uM2hrB

value: HepG2 cells

tag: genotype

value: ATCC HB-8065

tag: treatment

value: 5uM Pio, 2hr


title: CJ4_26_H_rep_4

source_name: Murine pancreatic fibroblasts_Co-cultured with Clone H

value: Co-cultured with Clone H


title: CJ4_02_H_rep_1


title: CJ4_17_E_rep_3

source_name: Murine pancreatic fibroblasts_Co-cultured with Clone E

value: Co-cultured with Clone E


title: CJ3_11_64_KPC1_co_rep_4

data_processing:
NB Dataset consists of three RNA sequencing runs. Some samples were sequenced in multiple runs, producing multiple fastq files. These should be combined during processing.
Sequencing reads were aligned to the mouse reference genome  using MapSplice (version 2.1.6)
Gene annotation was taken from Ensembl build 87. Read counts were determined by using the featureCounts function from the Bioconductor package Rsubread (version 1.16.1).
Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using the EdgeR package.
Genome_build: mm10
Supplementary_files_format_and_content: CSV files contain aligned read counts per sample, and normalised RPKM values.

source_name: KPC1 isolated from co-culture with fibroblasts

extract_protocol:
Isolated tumour cells were pelleted and lysed in QIAgen RLT buffer. RNA was extracted using a Qiagen RNeasy plus kit.
Indexed PolyA libraries were prepared using 200ng of total RNA and 14 cycles of amplification with the Agilent SureSelect Strand Specific RNA Library Prep Kit for Illumina Sequencing.
Paired-end 76 base-pair sequencing was carried out by clustering 2pM of the pooled libraries on a NextSeq 500 sequencer

tag: strain background

value: C57BL/6 mixed

value: Murine pancreatic tumour cells

tag: cell subtype


title: CJ4_10_H_rep_2


title: CJ4_01_E_rep_1


title: CJ4_18_H_rep_3


title: CJ3_59_63_KPC1_mono_rep_4

source_name: KPC1 mono-cultured

value: KPC1 mono-cultured


title: CJ3_63_56_V_co_rep_4

source_name: Clone V isolated from co-culture with fibroblasts


title: CJ4_25_E_rep_4


title: CJ3_56_59_KPC1_mono_rep_2


title: CJ3_51_62_KPC1_co_rep_3

value: KPC1 isolated from co-culture with fibroblasts


title: CJ3_10_58_KPC1_co_rep_1


title: CJ3_50_57_KPC1_mono_rep_1


title: CJ4_09_E_rep_2


title: CJ3_20_61_KPC1_mono_rep_3


title: CJ3_35_60_KPC1_co_rep_2


title: CJ3_8_54_V_co_rep_3

value: Clone V isolated from co-culture with fibroblasts


title: CJ3_49_50_V_co_rep_1


title: CJ4_24_KPC1_rep_3

value: Murine pancreatic fibroblasts


title: CJ3_13_53_V_mono_rep_3

source_name: Clone V mono-cultured

value: Clone V mono-cultured


title: CJ3_4_55_V_mono_rep_4


title: CJ3_3_51_V_mono_rep_2


title: CJ3_41_49_V_mono_rep_1


title: CJ3_33_52_V_co_rep_2


title: CJ3_12_44_T_co_rep_2

source_name: Clone T isolated from co-culture with fibroblasts


title: CJ3_48_48_T_co_rep_4

value: Clone T isolated from co-culture with fibroblasts


title: CJ3_16_46_T_co_rep_3


title: CJ3_57_42_T_co_rep_1


title: CJ3_15_47_T_mono_rep_4

source_name: Clone T mono-cultured

value: Clone T mono-cultured


title: CJ3_55_45_T_mono_rep_3


title: CJ3_9_38_N_co_rep_3

source_name: Clone N isolated from co-culture with fibroblasts


title: CJ3_45_41_T_mono_rep_1


title: CJ3_21_43_T_mono_rep_2


title: CJ3_24_40_N_co_rep_4

value: Clone N isolated from co-culture with fibroblasts


title: CJ3_38_35_N_mono_rep_2

source_name: Clone N mono-cultured

value: Clone N mono-cultured


title: CJ3_54_34_N_co_rep_1


title: CJ3_34_36_N_co_rep_2


title: CJ3_40_32_M_co_rep_4

source_name: Clone M isolated from co-culture with fibroblasts


title: CJ3_25_33_N_mono_rep_1


title: CJ3_27_37_N_mono_rep_3


title: CJ3_2_29_M_mono_rep_3

source_name: Clone M mono-cultured

value: Clone M mono-cultured


title: CJ3_44_27_M_mono_rep_2


title: CJ3_36_24_K_co_rep_4

source_name: Clone K isolated from co-culture with fibroblasts


title: CJ3_5_31_M_mono_rep_4


title: CJ3_32_26_M_co_rep_1

value: Clone M isolated from co-culture with fibroblasts


title: CJ3_60_22_K_co_rep_3

value: Clone K isolated from co-culture with fibroblasts


title: CJ3_29_39_N_mono_rep_4


title: CJ3_46_28_M_co_rep_2


title: CJ3_26_30_M_co_rep_3


title: CJ3_14_25_M_mono_rep1


title: CJ3_17_16_H_co_rep_4

source_name: Clone H isolated from co-culture with fibroblasts


title: CJ3_62_21_K_mono_rep_3

source_name: Clone K mono-cultured

value: Clone K mono-cultured


title: CJ3_58_12_H_co_rep_2

value: Clone H isolated from co-culture with fibroblasts


title: CJ3_47_17_K_mono_rep_1


title: CJ3_53_18_K_co_rep_1


title: CJ3_22_10_H_co_rep_1


title: CJ3_7_14_H_co_rep_3


title: CJ3_64_19_K_mono_rep_2


title: CJ3_19_13_H_mono_rep_3

source_name: Clone H mono-cultured

value: Clone H mono-cultured


title: CJ3_52_15_H_mono_rep_4

value: C57BL/6 mixed


title: CJ3_30_11_H_mono_rep_2

value: Murine pancreatic tumour cells


title: CJ3_61_23_K_mono_rep_4


title: CJ3_23_9_H_mono_rep_1


title: CJ3_42_8_E_co_rep_4

source_name: Clone E isolated from co-culture with fibroblasts


title: CJ3_39_4_E_co_rep_2

value: Clone E isolated from co-culture with fibroblasts


title: CJ3_37_6_E_co_rep_3


title: CJ3_43_2_E_co_rep_1


title: CJ3_6_7_E_mono_rep_4

source_name: Clone E mono-cultured

value: Clone E mono-cultured


title: CJ3_18_5_E_mono_rep_3


title: CJ3_28_3_E_mono_rep_2


title: CJ3_1_1_E_mono_rep_1


title: Sample 2208_PS_VE_Plate9_H07

city: Cambridge

first: Felix

last: Krueger

address: Granta Park

institute: Altos Labs

data_processing:
Libraries were sequenced on the Illumina Nextseq500 platform using the default RTA analysis software.
RNA-seq libraries were aligned to the GRCm38 mouse genome build using HiSat270 (v2.1.0) using options --dta --sp 1000,1000 --no-mixed --no-discordant, yielding a mean of 611,000 aligned reads per cell.
Subsequently, gene expression counts were quantified from the mapped reads using featureCounts with the Ensembl gene annotation (version 87). Only protein-coding genes matching canonical chromosomes were considered. The read counts were log-transformed and size-factor adjusted.
Genome_build: GRCm38
Supplementary_files_format_and_content: A table of genes (rows) x cells (columns). The first column is the ensembl gene ID

source_name: Early embryo

extract_protocol:
Single-cells were sorted either manually (E4.5, E5.5) or using flow cytometry (E6.5, E7.5) into 96 well PCR plates containing 2.5μl of methylase reaction buffer (1 × M.CviPI Reaction buffer (NEB), 2 U M.CviPI (NEB), 160 μM S-adenosylmethionine (NEB), 1 U μl−1 RNasein (Promega), 0.1% IGEPAL (Sigma)). Samples were incubated for 15 minutes at 37°C to methylate accessible chromatin before the reaction was stopped with the addition of RLT plus buffer (Qiagen) and samples frozen down and stored at -80°C prior to processing.
Poly-A RNA was captured on oligo-dT conjugated to magnetic beads and amplified cDNA was prepared according to the G&T-seq and Smartseq2 protocols. The lysate containing gDNA was purified on AMPureXP beads before bisulfite-seq libraries were prepared according to the scBS-seq protocol.

tag: developmental stage


title: Sample 2209_PS_VE_Plate9_H08


title: Sample 2207_PS_VE_Plate9_H06


title: Sample 2206_PS_VE_Plate9_H05


title: Sample 2210_PS_VE_Plate9_H09


title: Sample 2212_PS_VE_Plate9_H11


title: Sample 2213_PS_VE_Plate9_H12


title: Sample 2211_PS_VE_Plate9_H10


title: CJ3_31_20_K_co_rep_2


title: Sample 2205_PS_VE_Plate9_H04


title: Sample 2204_PS_VE_Plate9_H03


title: Sample 2201_PS_VE_Plate9_G12


title: Sample 2202_PS_VE_Plate9_H01


title: Sample 2200_PS_VE_Plate9_G11


title: Sample 2196_PS_VE_Plate9_G07


title: Sample 2197_PS_VE_Plate9_G08


title: Sample 2198_PS_VE_Plate9_G09


title: Sample 2199_PS_VE_Plate9_G10


title: Sample 2195_PS_VE_Plate9_G06


title: Sample 2188_PS_VE_Plate9_F11


title: Sample 2187_PS_VE_Plate9_F10


title: Sample 2191_PS_VE_Plate9_G02


title: Sample 2192_PS_VE_Plate9_G03


title: Sample 2190_PS_VE_Plate9_G01


title: Sample 2183_PS_VE_Plate9_F06


title: Sample 2186_PS_VE_Plate9_F09


title: Sample 2180_PS_VE_Plate9_F03


title: Sample 2179_PS_VE_Plate9_F02


title: Sample 2181_PS_VE_Plate9_F04


title: Sample 2185_PS_VE_Plate9_F08


title: Sample 2184_PS_VE_Plate9_F07


title: Sample 2203_PS_VE_Plate9_H02


title: Sample 2193_PS_VE_Plate9_G04


title: Sample 2178_PS_VE_Plate9_F01


title: Sample 2189_PS_VE_Plate9_F12


title: Sample 2176_PS_VE_Plate9_E11


title: Sample 2175_PS_VE_Plate9_E10


title: Sample 2174_PS_VE_Plate9_E09


title: Sample 2177_PS_VE_Plate9_E12


title: Sample 2173_PS_VE_Plate9_E08


title: Sample 2194_PS_VE_Plate9_G05


title: Sample 2182_PS_VE_Plate9_F05


title: Sample 2172_PS_VE_Plate9_E07


title: Sample 2170_PS_VE_Plate9_E05


title: Sample 2171_PS_VE_Plate9_E06


title: Sample 2169_PS_VE_Plate9_E04


title: Sample 2167_PS_VE_Plate9_E02


title: Sample 2168_PS_VE_Plate9_E03


title: Sample 2163_PS_VE_Plate9_D10


title: Sample 2164_PS_VE_Plate9_D11


title: Sample 2162_PS_VE_Plate9_D09


title: Sample 2161_PS_VE_Plate9_D08


title: Sample 2159_PS_VE_Plate9_D06


title: Sample 2160_PS_VE_Plate9_D07


title: Sample 2158_PS_VE_Plate9_D05


title: Sample 2155_PS_VE_Plate9_D02


title: Sample 2154_PS_VE_Plate9_D01


title: Sample 2157_PS_VE_Plate9_D04


title: Sample 2153_PS_VE_Plate9_C12


title: Sample 2151_PS_VE_Plate9_C10


title: Sample 2156_PS_VE_Plate9_D03


title: Sample 2146_PS_VE_Plate9_C05


title: Sample 2149_PS_VE_Plate9_C08


title: Sample 2142_PS_VE_Plate9_C01


title: Sample 2147_PS_VE_Plate9_C06


title: Sample 2145_PS_VE_Plate9_C04


title: Sample 2148_PS_VE_Plate9_C07


title: Sample 2143_PS_VE_Plate9_C02


title: Sample 2144_PS_VE_Plate9_C03


title: Sample 2141_PS_VE_Plate9_B12


title: Sample 2150_PS_VE_Plate9_C09


title: Sample 2139_PS_VE_Plate9_B10


title: Sample 2140_PS_VE_Plate9_B11


title: Sample 2165_PS_VE_Plate9_D12


title: Sample 2134_PS_VE_Plate9_B05


title: Sample 2135_PS_VE_Plate9_B06


title: Sample 2133_PS_VE_Plate9_B04


title: Sample 2152_PS_VE_Plate9_C11


title: Sample 2132_PS_VE_Plate9_B03


title: Sample 2166_PS_VE_Plate9_E01


title: Sample 2130_PS_VE_Plate9_B01


title: Sample 2129_PS_VE_Plate9_A12


title: Sample 2128_PS_VE_Plate9_A11


title: Sample 2138_PS_VE_Plate9_B09


title: Sample 2127_PS_VE_Plate9_A10


title: Sample 2126_PS_VE_Plate9_A09


title: Sample 2118_PS_VE_Plate9_A01


title: Sample 2121_PS_VE_Plate9_A04


title: Sample 2120_PS_VE_Plate9_A03


title: Sample 2137_PS_VE_Plate9_B08


title: Sample 2116_PS_VE_Plate8_H11


title: Sample 2125_PS_VE_Plate9_A08


title: Sample 2114_PS_VE_Plate8_H09


title: Sample 2115_PS_VE_Plate8_H10


title: Sample 2122_PS_VE_Plate9_A05


title: Sample 2111_PS_VE_Plate8_H06


title: Sample 2112_PS_VE_Plate8_H07


title: Sample 2113_PS_VE_Plate8_H08


title: Sample 2110_PS_VE_Plate8_H05


title: Sample 2131_PS_VE_Plate9_B02


title: Sample 2107_PS_VE_Plate8_H02


title: Sample 2136_PS_VE_Plate9_B07


title: Sample 2124_PS_VE_Plate9_A07


title: Sample 2104_PS_VE_Plate8_G11


title: Sample 2106_PS_VE_Plate8_H01


title: Sample 2105_PS_VE_Plate8_G12


title: Sample 2119_PS_VE_Plate9_A02


title: Sample 2117_PS_VE_Plate8_H12


title: Sample 2102_PS_VE_Plate8_G09


title: Sample 2123_PS_VE_Plate9_A06


title: Sample 2101_PS_VE_Plate8_G08


title: Sample 2100_PS_VE_Plate8_G07


title: Sample 2099_PS_VE_Plate8_G06


title: Sample 2109_PS_VE_Plate8_H04


title: Sample 2094_PS_VE_Plate8_G01


title: Sample 2093_PS_VE_Plate8_F12


title: Sample 2095_PS_VE_Plate8_G02


title: Sample 2092_PS_VE_Plate8_F11


title: Sample 2108_PS_VE_Plate8_H03


title: Sample 2090_PS_VE_Plate8_F09


title: Sample 2088_PS_VE_Plate8_F07


title: Sample 2097_PS_VE_Plate8_G04


title: Sample 2085_PS_VE_Plate8_F04


title: Sample 2103_PS_VE_Plate8_G10


title: Sample 2082_PS_VE_Plate8_F01


title: Sample 2083_PS_VE_Plate8_F02


title: Sample 2098_PS_VE_Plate8_G05


title: Sample 2078_PS_VE_Plate8_E09


title: Sample 2096_PS_VE_Plate8_G03


title: Sample 2084_PS_VE_Plate8_F03


title: Sample 2077_PS_VE_Plate8_E08


title: Sample 2091_PS_VE_Plate8_F10


title: Sample 2074_PS_VE_Plate8_E05


title: Sample 2089_PS_VE_Plate8_F08


title: Sample 2087_PS_VE_Plate8_F06


title: Sample 2073_PS_VE_Plate8_E04


title: Sample 2072_PS_VE_Plate8_E03


title: Sample 2086_PS_VE_Plate8_F05


title: Sample 2069_PS_VE_Plate8_D12


title: Sample 2071_PS_VE_Plate8_E02


title: Sample 2070_PS_VE_Plate8_E01


title: Sample 2066_PS_VE_Plate8_D09


title: Sample 2062_PS_VE_Plate8_D05


title: Sample 2068_PS_VE_Plate8_D11


title: Sample 2081_PS_VE_Plate8_E12


title: Sample 2065_PS_VE_Plate8_D08


title: Sample 2080_PS_VE_Plate8_E11


title: Sample 2067_PS_VE_Plate8_D10


title: Sample 2064_PS_VE_Plate8_D07


title: Sample 2079_PS_VE_Plate8_E10


title: Sample 2063_PS_VE_Plate8_D06


title: Sample 2061_PS_VE_Plate8_D04


title: Sample 2057_PS_VE_Plate8_C12


title: Sample 2058_PS_VE_Plate8_D01


title: Sample 2059_PS_VE_Plate8_D02


title: Sample 2054_PS_VE_Plate8_C09


title: Sample 2060_PS_VE_Plate8_D03


title: Sample 2076_PS_VE_Plate8_E07


title: Sample 2055_PS_VE_Plate8_C10


title: Sample 2050_PS_VE_Plate8_C05


title: Sample 2052_PS_VE_Plate8_C07


title: Sample 2051_PS_VE_Plate8_C06


title: Sample 2056_PS_VE_Plate8_C11


title: Sample 2048_PS_VE_Plate8_C03


title: Sample 2053_PS_VE_Plate8_C08


title: Sample 2047_PS_VE_Plate8_C02


title: Sample 2046_PS_VE_Plate8_C01


title: Sample 2045_PS_VE_Plate8_B12


title: Sample 2044_PS_VE_Plate8_B11


title: Sample 2043_PS_VE_Plate8_B10


title: Sample 2042_PS_VE_Plate8_B09


title: Sample 2041_PS_VE_Plate8_B08


title: Sample 2036_PS_VE_Plate8_B03


title: Sample 2040_PS_VE_Plate8_B07


title: Sample 2038_PS_VE_Plate8_B05


title: Sample 2035_PS_VE_Plate8_B02


title: Sample 2033_PS_VE_Plate8_A12


title: Sample 2031_PS_VE_Plate8_A10


title: Sample 2032_PS_VE_Plate8_A11


title: Sample 2030_PS_VE_Plate8_A09


title: Sample 2028_PS_VE_Plate8_A07


title: Sample 2049_PS_VE_Plate8_C04


title: Sample 2029_PS_VE_Plate8_A08


title: Sample 2027_PS_VE_Plate8_A06


title: Sample 2024_PS_VE_Plate8_A03


title: Sample 2025_PS_VE_Plate8_A04


title: Sample 2026_PS_VE_Plate8_A05


title: Sample 2019_PS_VE_Plate7_H10


title: Sample 2020_PS_VE_Plate7_H11


title: Sample 2021_PS_VE_Plate7_H12


title: Sample 2022_PS_VE_Plate8_A01


title: Sample 2018_PS_VE_Plate7_H09


title: Sample 2023_PS_VE_Plate8_A02


title: Sample 2017_PS_VE_Plate7_H08


title: Sample 2039_PS_VE_Plate8_B06


title: Sample 2037_PS_VE_Plate8_B04


title: Sample 2014_PS_VE_Plate7_H05


title: Sample 2013_PS_VE_Plate7_H04


title: Sample 2015_PS_VE_Plate7_H06


title: Sample 2016_PS_VE_Plate7_H07


title: Sample 2010_PS_VE_Plate7_H01


title: Sample 2034_PS_VE_Plate8_B01


title: Sample 2007_PS_VE_Plate7_G10


title: Sample 2008_PS_VE_Plate7_G11


title: Sample 2006_PS_VE_Plate7_G09


title: Sample 2009_PS_VE_Plate7_G12


title: Sample 2011_PS_VE_Plate7_H02


title: Sample 2005_PS_VE_Plate7_G08


title: Sample 2001_PS_VE_Plate7_G04


title: Sample 2002_PS_VE_Plate7_G05


title: Sample 2004_PS_VE_Plate7_G07


title: Sample 1998_PS_VE_Plate7_G01


title: Sample 2003_PS_VE_Plate7_G06


title: Sample 1999_PS_VE_Plate7_G02


title: Sample 2000_PS_VE_Plate7_G03


title: Sample 1991_PS_VE_Plate7_F06


title: Sample 1994_PS_VE_Plate7_F09


title: Sample 1992_PS_VE_Plate7_F07


title: Sample 1995_PS_VE_Plate7_F10


title: Sample 1993_PS_VE_Plate7_F08


title: Sample 1997_PS_VE_Plate7_F12


title: Sample 1996_PS_VE_Plate7_F11


title: Sample 2012_PS_VE_Plate7_H03


title: Sample 1990_PS_VE_Plate7_F05


title: Sample 1989_PS_VE_Plate7_F04


title: Sample 1988_PS_VE_Plate7_F03


title: Sample 1985_PS_VE_Plate7_E12


title: Sample 1986_PS_VE_Plate7_F01


title: Sample 1987_PS_VE_Plate7_F02


title: Sample 1984_PS_VE_Plate7_E11


title: Sample 1981_PS_VE_Plate7_E08


title: Sample 1982_PS_VE_Plate7_E09


title: Sample 1983_PS_VE_Plate7_E10


title: Sample 1979_PS_VE_Plate7_E06


title: Sample 1977_PS_VE_Plate7_E04


title: Sample 1978_PS_VE_Plate7_E05


title: Sample 1980_PS_VE_Plate7_E07


title: Sample 1976_PS_VE_Plate7_E03


title: Sample 1975_PS_VE_Plate7_E02


title: Sample 1973_PS_VE_Plate7_D12


title: Sample 1972_PS_VE_Plate7_D11


title: Sample 1974_PS_VE_Plate7_E01


title: Sample 1971_PS_VE_Plate7_D10


title: Sample 1970_PS_VE_Plate7_D09


title: Sample 1962_PS_VE_Plate7_D01


title: Sample 1966_PS_VE_Plate7_D05


title: Sample 1968_PS_VE_Plate7_D07


title: Sample 1964_PS_VE_Plate7_D03


title: Sample 1961_PS_VE_Plate7_C12


title: Sample 1959_PS_VE_Plate7_C10


title: Sample 1967_PS_VE_Plate7_D06


title: Sample 1969_PS_VE_Plate7_D08


title: Sample 1958_PS_VE_Plate7_C09


title: Sample 1956_PS_VE_Plate7_C07


title: Sample 1960_PS_VE_Plate7_C11


title: Sample 1957_PS_VE_Plate7_C08


title: Sample 1953_PS_VE_Plate7_C04


title: Sample 1951_PS_VE_Plate7_C02


title: Sample 1955_PS_VE_Plate7_C06


title: Sample 1963_PS_VE_Plate7_D02


title: Sample 1952_PS_VE_Plate7_C03


title: Sample 1948_PS_VE_Plate7_B11


title: Sample 1950_PS_VE_Plate7_C01


title: Sample 1949_PS_VE_Plate7_B12


title: Sample 1947_PS_VE_Plate7_B10


title: Sample 1946_PS_VE_Plate7_B09


title: Sample 1954_PS_VE_Plate7_C05


title: Sample 1945_PS_VE_Plate7_B08


title: Sample 1965_PS_VE_Plate7_D04


title: Sample 1944_PS_VE_Plate7_B07


title: Sample 1943_PS_VE_Plate7_B06


title: Sample 1941_PS_VE_Plate7_B04


title: Sample 1934_PS_VE_Plate7_A09


title: Sample 1939_PS_VE_Plate7_B02


title: Sample 1940_PS_VE_Plate7_B03


title: Sample 1935_PS_VE_Plate7_A10


title: Sample 1936_PS_VE_Plate7_A11


title: Sample 1938_PS_VE_Plate7_B01


title: Sample 1942_PS_VE_Plate7_B05


title: Sample 1937_PS_VE_Plate7_A12


title: Sample 1928_PS_VE_Plate7_A03


title: Sample 1931_PS_VE_Plate7_A06


title: Sample 1927_PS_VE_Plate7_A02


title: Sample 1929_PS_VE_Plate7_A04


title: Sample 1932_PS_VE_Plate7_A07


title: Sample 1925_PS_VE_Plate6_H12


title: Sample 1933_PS_VE_Plate7_A08


title: Sample 1926_PS_VE_Plate7_A01


title: Sample 1930_PS_VE_Plate7_A05


title: Sample 1924_PS_VE_Plate6_H11


title: Sample 1920_PS_VE_Plate6_H07


title: Sample 1918_PS_VE_Plate6_H05


title: Sample 1923_PS_VE_Plate6_H10


title: Sample 1922_PS_VE_Plate6_H09


title: Sample 1919_PS_VE_Plate6_H06


title: Sample 1914_PS_VE_Plate6_H01


title: Sample 1915_PS_VE_Plate6_H02


title: Sample 1916_PS_VE_Plate6_H03


title: Sample 1913_PS_VE_Plate6_G12


title: Sample 1911_PS_VE_Plate6_G10


title: Sample 1917_PS_VE_Plate6_H04


title: Sample 1912_PS_VE_Plate6_G11


title: Sample 1910_PS_VE_Plate6_G09


title: Sample 1909_PS_VE_Plate6_G08


title: Sample 1907_PS_VE_Plate6_G06


title: Sample 1908_PS_VE_Plate6_G07


title: Sample 1904_PS_VE_Plate6_G03


title: Sample 1903_PS_VE_Plate6_G02


title: Sample 1906_PS_VE_Plate6_G05


title: Sample 1921_PS_VE_Plate6_H08


title: Sample 1905_PS_VE_Plate6_G04


title: Sample 1901_PS_VE_Plate6_F12


title: Sample 1900_PS_VE_Plate6_F11


title: Sample 1898_PS_VE_Plate6_F09


title: Sample 1897_PS_VE_Plate6_F08


title: Sample 1896_PS_VE_Plate6_F07


title: Sample 1902_PS_VE_Plate6_G01


title: Sample 1899_PS_VE_Plate6_F10


title: Sample 1895_PS_VE_Plate6_F06


title: Sample 1891_PS_VE_Plate6_F02


title: Sample 1893_PS_VE_Plate6_F04


title: Sample 1892_PS_VE_Plate6_F03


title: Sample 1890_PS_VE_Plate6_F01


title: Sample 1889_PS_VE_Plate6_E12


title: Sample 1888_PS_VE_Plate6_E11


title: Sample 1887_PS_VE_Plate6_E10


title: Sample 2075_PS_VE_Plate8_E06


title: Sample 1884_PS_VE_Plate6_E07


title: Sample 1881_PS_VE_Plate6_E04


title: Sample 1880_PS_VE_Plate6_E03


title: Sample 1883_PS_VE_Plate6_E06


title: Sample 1879_PS_VE_Plate6_E02


title: Sample 1882_PS_VE_Plate6_E05


title: Sample 1877_PS_VE_Plate6_D12


title: Sample 1874_PS_VE_Plate6_D09


title: Sample 1875_PS_VE_Plate6_D10


title: Sample 1876_PS_VE_Plate6_D11


title: Sample 1873_PS_VE_Plate6_D08


title: Sample 1871_PS_VE_Plate6_D06


title: Sample 1878_PS_VE_Plate6_E01


title: Sample 1869_PS_VE_Plate6_D04


title: Sample 1864_PS_VE_Plate6_C11


title: Sample 1868_PS_VE_Plate6_D03


title: Sample 1867_PS_VE_Plate6_D02


title: Sample 1862_PS_VE_Plate6_C09


title: Sample 1866_PS_VE_Plate6_D01


title: Sample 1863_PS_VE_Plate6_C10


title: Sample 1894_PS_VE_Plate6_F05


title: Sample 1865_PS_VE_Plate6_C12


title: Sample 1885_PS_VE_Plate6_E08


title: Sample 1858_PS_VE_Plate6_C05


title: Sample 1860_PS_VE_Plate6_C07


title: Sample 1861_PS_VE_Plate6_C08


title: Sample 1857_PS_VE_Plate6_C04


title: Sample 1859_PS_VE_Plate6_C06


title: Sample 1856_PS_VE_Plate6_C03


title: Sample 1854_PS_VE_Plate6_C01


title: Sample 1855_PS_VE_Plate6_C02


title: Sample 1870_PS_VE_Plate6_D05


title: Sample 1853_PS_VE_Plate6_B12


title: Sample 1872_PS_VE_Plate6_D07


title: Sample 1852_PS_VE_Plate6_B11


title: Sample 1848_PS_VE_Plate6_B07


title: Sample 1851_PS_VE_Plate6_B10


title: Sample 1847_PS_VE_Plate6_B06


title: Sample 1846_PS_VE_Plate6_B05


title: Sample 1843_PS_VE_Plate6_B02


title: Sample 1842_PS_VE_Plate6_B01


title: Sample 1841_PS_VE_Plate6_A12


title: Sample 1850_PS_VE_Plate6_B09


title: Sample 1844_PS_VE_Plate6_B03


title: Sample 1839_PS_VE_Plate6_A10


title: Sample 1840_PS_VE_Plate6_A11


title: Sample 1836_PS_VE_Plate6_A07


title: Sample 1845_PS_VE_Plate6_B04


title: Sample 1838_PS_VE_Plate6_A09


title: Sample 1835_PS_VE_Plate6_A06


title: Sample 1832_PS_VE_Plate6_A03


title: Sample 1833_PS_VE_Plate6_A04


title: Sample 1830_PS_VE_Plate6_A01


title: Sample 1837_PS_VE_Plate6_A08


title: Sample 1834_PS_VE_Plate6_A05


title: Sample 1827_PS_VE_Plate5_H10


title: Sample 1825_PS_VE_Plate5_H08


title: Sample 1826_PS_VE_Plate5_H09


title: Sample 1822_PS_VE_Plate5_H05


title: Sample 1824_PS_VE_Plate5_H07


title: Sample 1823_PS_VE_Plate5_H06


title: Sample 1819_PS_VE_Plate5_H02


title: Sample 1818_PS_VE_Plate5_H01


title: Sample 1820_PS_VE_Plate5_H03


title: Sample 1886_PS_VE_Plate6_E09


title: Sample 1815_PS_VE_Plate5_G10


title: Sample 1817_PS_VE_Plate5_G12


title: Sample 1813_PS_VE_Plate5_G08


title: Sample 1816_PS_VE_Plate5_G11


title: Sample 1831_PS_VE_Plate6_A02


title: Sample 1828_PS_VE_Plate5_H11


title: Sample 1808_PS_VE_Plate5_G03


title: Sample 1849_PS_VE_Plate6_B08


title: Sample 1805_PS_VE_Plate5_F12


title: Sample 1804_PS_VE_Plate5_F11


title: Sample 1810_PS_VE_Plate5_G05


title: Sample 1803_PS_VE_Plate5_F10


title: Sample 1814_PS_VE_Plate5_G09


title: Sample 1797_PS_VE_Plate5_F04


title: Sample 1800_PS_VE_Plate5_F07


title: Sample 1799_PS_VE_Plate5_F06


title: Sample 1801_PS_VE_Plate5_F08


title: Sample 1798_PS_VE_Plate5_F05


title: Sample 1802_PS_VE_Plate5_F09


title: Sample 1806_PS_VE_Plate5_G01


title: Sample 1829_PS_VE_Plate5_H12


title: Sample 1821_PS_VE_Plate5_H04


title: Sample 1812_PS_VE_Plate5_G07


title: Sample 1809_PS_VE_Plate5_G04


title: Sample 1792_PS_VE_Plate5_E11


title: Sample 1791_PS_VE_Plate5_E10


title: Sample 1807_PS_VE_Plate5_G02


title: Sample 1790_PS_VE_Plate5_E09


title: Sample 1811_PS_VE_Plate5_G06


title: Sample 1789_PS_VE_Plate5_E08


title: Sample 1786_PS_VE_Plate5_E05


title: Sample 1788_PS_VE_Plate5_E07


title: Sample 1787_PS_VE_Plate5_E06


title: Sample 1785_PS_VE_Plate5_E04


title: Sample 1784_PS_VE_Plate5_E03


title: Sample 1793_PS_VE_Plate5_E12


title: Sample 1781_PS_VE_Plate5_D12


title: Sample 1783_PS_VE_Plate5_E02


title: Sample 1775_PS_VE_Plate5_D06


title: Sample 1780_PS_VE_Plate5_D11


title: Sample 1772_PS_VE_Plate5_D03


title: Sample 1778_PS_VE_Plate5_D09


title: Sample 1794_PS_VE_Plate5_F01


title: Sample 1779_PS_VE_Plate5_D10


title: Sample 1795_PS_VE_Plate5_F02


title: Sample 1770_PS_VE_Plate5_D01


title: Sample 1776_PS_VE_Plate5_D07


title: Sample 1796_PS_VE_Plate5_F03


title: Sample 1769_PS_VE_Plate5_C12


title: Sample 1771_PS_VE_Plate5_D02


title: Sample 1766_PS_VE_Plate5_C09


title: Sample 1773_PS_VE_Plate5_D04


title: Sample 1764_PS_VE_Plate5_C07


title: Sample 1765_PS_VE_Plate5_C08


title: Sample 1782_PS_VE_Plate5_E01


title: Sample 1759_PS_VE_Plate5_C02


title: Sample 1761_PS_VE_Plate5_C04


title: Sample 1760_PS_VE_Plate5_C03


title: Sample 1762_PS_VE_Plate5_C05


title: Sample 1756_PS_VE_Plate5_B11


title: Sample 1777_PS_VE_Plate5_D08


title: Sample 1755_PS_VE_Plate5_B10


title: Sample 1758_PS_VE_Plate5_C01


title: Sample 1774_PS_VE_Plate5_D05


title: Sample 1754_PS_VE_Plate5_B09


title: Sample 1753_PS_VE_Plate5_B08


title: Sample 1752_PS_VE_Plate5_B07


title: Sample 1749_PS_VE_Plate5_B04


title: Sample 1750_PS_VE_Plate5_B05


title: Sample 1767_PS_VE_Plate5_C10


title: Sample 1768_PS_VE_Plate5_C11


title: Sample 1763_PS_VE_Plate5_C06


title: Sample 1746_PS_VE_Plate5_B01


title: Sample 1744_PS_VE_Plate5_A11


title: Sample 1745_PS_VE_Plate5_A12


title: Sample 1743_PS_VE_Plate5_A10


title: Sample 1747_PS_VE_Plate5_B02


title: Sample 1741_PS_VE_Plate5_A08


title: Sample 1740_PS_VE_Plate5_A07


title: Sample 1742_PS_VE_Plate5_A09


title: Sample 1739_PS_VE_Plate5_A06


title: Sample 1737_PS_VE_Plate5_A04


title: Sample 1738_PS_VE_Plate5_A05


title: Sample 1736_PS_VE_Plate5_A03


title: Sample 1734_PS_VE_Plate5_A01


title: Sample 1757_PS_VE_Plate5_B12


title: Sample 1733_PS_VE_Plate4_H12


title: Sample 1730_PS_VE_Plate4_H09


title: Sample 1751_PS_VE_Plate5_B06


title: Sample 1732_PS_VE_Plate4_H11


title: Sample 1735_PS_VE_Plate5_A02


title: Sample 1725_PS_VE_Plate4_H04


title: Sample 1729_PS_VE_Plate4_H08


title: Sample 1723_PS_VE_Plate4_H02


title: Sample 1728_PS_VE_Plate4_H07


title: Sample 1727_PS_VE_Plate4_H06


title: Sample 1726_PS_VE_Plate4_H05


title: Sample 1722_PS_VE_Plate4_H01


title: Sample 1719_PS_VE_Plate4_G10


title: Sample 1717_PS_VE_Plate4_G08


title: Sample 1721_PS_VE_Plate4_G12


title: Sample 1718_PS_VE_Plate4_G09


title: Sample 1748_PS_VE_Plate5_B03


title: Sample 1713_PS_VE_Plate4_G04


title: Sample 1716_PS_VE_Plate4_G07


title: Sample 1714_PS_VE_Plate4_G05


title: Sample 1724_PS_VE_Plate4_H03


title: Sample 1710_PS_VE_Plate4_G01


title: Sample 1709_PS_VE_Plate4_F12


title: Sample 1705_PS_VE_Plate4_F08


title: Sample 1708_PS_VE_Plate4_F11


title: Sample 1707_PS_VE_Plate4_F10


title: Sample 1706_PS_VE_Plate4_F09


title: Sample 1731_PS_VE_Plate4_H10


title: Sample 1704_PS_VE_Plate4_F07


title: Sample 1720_PS_VE_Plate4_G11


title: Sample 1703_PS_VE_Plate4_F06


title: Sample 1715_PS_VE_Plate4_G06


title: Sample 1695_PS_VE_Plate4_E10


title: Sample 1696_PS_VE_Plate4_E11


title: Sample 1693_PS_VE_Plate4_E08


title: Sample 1698_PS_VE_Plate4_F01


title: Sample 1699_PS_VE_Plate4_F02


title: Sample 1694_PS_VE_Plate4_E09


title: Sample 1712_PS_VE_Plate4_G03


title: Sample 1711_PS_VE_Plate4_G02


title: Sample 1688_PS_VE_Plate4_E03


title: Sample 1690_PS_VE_Plate4_E05


title: Sample 1689_PS_VE_Plate4_E04


title: Sample 1691_PS_VE_Plate4_E06


title: Sample 1687_PS_VE_Plate4_E02


title: Sample 1702_PS_VE_Plate4_F05


title: Sample 1686_PS_VE_Plate4_E01


title: Sample 1700_PS_VE_Plate4_F03


title: Sample 1684_PS_VE_Plate4_D11


title: Sample 1685_PS_VE_Plate4_D12


title: Sample 1683_PS_VE_Plate4_D10


title: Sample 1681_PS_VE_Plate4_D08


title: Sample 1682_PS_VE_Plate4_D09


title: Sample 1697_PS_VE_Plate4_E12


title: Sample 1680_PS_VE_Plate4_D07


title: Sample 1679_PS_VE_Plate4_D06


title: Sample 1701_PS_VE_Plate4_F04


title: Sample 1678_PS_VE_Plate4_D05


title: Sample 1677_PS_VE_Plate4_D04


title: Sample 1675_PS_VE_Plate4_D02


title: Sample 1676_PS_VE_Plate4_D03


title: Sample 1672_PS_VE_Plate4_C11


title: Sample 1671_PS_VE_Plate4_C10


title: Sample 1670_PS_VE_Plate4_C09


title: Sample 1674_PS_VE_Plate4_D01


title: Sample 1669_PS_VE_Plate4_C08


title: Sample 1668_PS_VE_Plate4_C07


title: Sample 1673_PS_VE_Plate4_C12


title: Sample 1667_PS_VE_Plate4_C06


title: Sample 1666_PS_VE_Plate4_C05


title: Sample 1665_PS_VE_Plate4_C04


title: Sample 1663_PS_VE_Plate4_C02


title: Sample 1664_PS_VE_Plate4_C03


title: Sample 1661_PS_VE_Plate4_B12


title: Sample 1660_PS_VE_Plate4_B11


title: Sample 1662_PS_VE_Plate4_C01


title: Sample 1659_PS_VE_Plate4_B10


title: Sample 1658_PS_VE_Plate4_B09


title: Sample 1655_PS_VE_Plate4_B06


title: Sample 1657_PS_VE_Plate4_B08


title: Sample 1650_PS_VE_Plate4_B01


title: Sample 1651_PS_VE_Plate4_B02


title: Sample 1652_PS_VE_Plate4_B03


title: Sample 1653_PS_VE_Plate4_B04


title: Sample 1648_PS_VE_Plate4_A11


title: Sample 1654_PS_VE_Plate4_B05


title: Sample 1649_PS_VE_Plate4_A12


title: Sample 1647_PS_VE_Plate4_A10


title: Sample 1646_PS_VE_Plate4_A09


title: Sample 1644_PS_VE_Plate4_A07


title: Sample 1645_PS_VE_Plate4_A08


title: Sample 1643_PS_VE_Plate4_A06


title: Sample 1640_PS_VE_Plate4_A03


title: Sample 1642_PS_VE_Plate4_A05


title: Sample 1641_PS_VE_Plate4_A04


title: Sample 1638_PS_VE_Plate4_A01


title: Sample 1639_PS_VE_Plate4_A02


title: Sample 1637_PS_VE_Plate3_H12


title: Sample 1633_PS_VE_Plate3_H08


title: Sample 1631_PS_VE_Plate3_H05


title: Sample 1630_PS_VE_Plate3_H04


title: Sample 1635_PS_VE_Plate3_H10


title: Sample 1634_PS_VE_Plate3_H09


title: Sample 1632_PS_VE_Plate3_H06


title: Sample 1636_PS_VE_Plate3_H11


title: Sample 1656_PS_VE_Plate4_B07


title: Sample 1629_PS_VE_Plate3_H03


title: Sample 1628_PS_VE_Plate3_H02


title: Sample 1625_PS_VE_Plate3_G11


title: Sample 1627_PS_VE_Plate3_H01


title: Sample 1626_PS_VE_Plate3_G12


title: Sample 1622_PS_VE_Plate3_G08


title: Sample 1623_PS_VE_Plate3_G09


title: Sample 1619_PS_VE_Plate3_G05


title: Sample 1621_PS_VE_Plate3_G07


title: Sample 1618_PS_VE_Plate3_G04


title: Sample 1617_PS_VE_Plate3_G03


title: Sample 1615_PS_VE_Plate3_G01


title: Sample 1614_PS_VE_Plate3_F12


title: Sample 1620_PS_VE_Plate3_G06


title: Sample 1616_PS_VE_Plate3_G02


title: Sample 1613_PS_VE_Plate3_F11


title: Sample 1610_PS_VE_Plate3_F08


title: Sample 1603_PS_VE_Plate3_F01


title: Sample 1605_PS_VE_Plate3_F03


title: Sample 1609_PS_VE_Plate3_F07


title: Sample 1606_PS_VE_Plate3_F04


title: Sample 1604_PS_VE_Plate3_F02


title: Sample 1608_PS_VE_Plate3_F06


title: Sample 1607_PS_VE_Plate3_F05


title: Sample 1601_PS_VE_Plate3_E11


title: Sample 1624_PS_VE_Plate3_G10


title: Sample 1600_PS_VE_Plate3_E10


title: Sample 1599_PS_VE_Plate3_E09


title: Sample 1602_PS_VE_Plate3_E12


title: Sample 1598_PS_VE_Plate3_E08


title: Sample 1597_PS_VE_Plate3_E07


title: Sample 1596_PS_VE_Plate3_E06


title: Sample 1590_PS_VE_Plate3_D12


title: Sample 1592_PS_VE_Plate3_E02


title: Sample 1587_PS_VE_Plate3_D09


title: Sample 1586_PS_VE_Plate3_D08


title: Sample 1595_PS_VE_Plate3_E05


title: Sample 1588_PS_VE_Plate3_D10


title: Sample 1591_PS_VE_Plate3_E01


title: Sample 1589_PS_VE_Plate3_D11


title: Sample 1585_PS_VE_Plate3_D07


title: Sample 1612_PS_VE_Plate3_F10


title: Sample 1594_PS_VE_Plate3_E04


title: Sample 1584_PS_VE_Plate3_D06


title: Sample 1593_PS_VE_Plate3_E03


title: Sample 1583_PS_VE_Plate3_D05


title: Sample 1578_PS_VE_Plate3_C12


title: Sample 1577_PS_VE_Plate3_C11


title: Sample 1579_PS_VE_Plate3_D01


title: Sample 1581_PS_VE_Plate3_D03


title: Sample 1580_PS_VE_Plate3_D02


title: Sample 1575_PS_VE_Plate3_C09


title: Sample 1576_PS_VE_Plate3_C10


title: Sample 1582_PS_VE_Plate3_D04


title: Sample 1574_PS_VE_Plate3_C08


title: Sample 1571_PS_VE_Plate3_C05


title: Sample 1573_PS_VE_Plate3_C07


title: Sample 1572_PS_VE_Plate3_C06


title: Sample 1570_PS_VE_Plate3_C04


title: Sample 1569_PS_VE_Plate3_C03


title: Sample 1566_PS_VE_Plate3_B12


title: Sample 1565_PS_VE_Plate3_B11


title: Sample 1558_PS_VE_Plate3_B04


title: Sample 1556_PS_VE_Plate3_B02


title: Sample 1564_PS_VE_Plate3_B10


title: Sample 1561_PS_VE_Plate3_B07


title: Sample 1559_PS_VE_Plate3_B05


title: Sample 1563_PS_VE_Plate3_B09


title: Sample 1557_PS_VE_Plate3_B03


title: Sample 1560_PS_VE_Plate3_B06


title: Sample 1562_PS_VE_Plate3_B08


title: Sample 1554_PS_VE_Plate3_A12


title: Sample 1552_PS_VE_Plate3_A10


title: Sample 1549_PS_VE_Plate3_A07


title: Sample 1551_PS_VE_Plate3_A09


title: Sample 1550_PS_VE_Plate3_A08


title: Sample 1555_PS_VE_Plate3_B01


title: Sample 1553_PS_VE_Plate3_A11


title: Sample 1548_PS_VE_Plate3_A06


title: Sample 1547_PS_VE_Plate3_A05


title: Sample 1545_PS_VE_Plate3_A03


title: Sample 1543_PS_VE_Plate3_A01


title: Sample 1546_PS_VE_Plate3_A04


title: Sample 1568_PS_VE_Plate3_C02


title: Sample 1567_PS_VE_Plate3_C01


title: Sample 1541_PS_VE_Plate2_H11


title: Sample 1542_PS_VE_Plate2_H12


title: Sample 1539_PS_VE_Plate2_H09


title: Sample 1540_PS_VE_Plate2_H10


title: Sample 1537_PS_VE_Plate2_H07


title: Sample 1536_PS_VE_Plate2_H06


title: Sample 1538_PS_VE_Plate2_H08


title: Sample 1692_PS_VE_Plate4_E07


title: Sample 1535_PS_VE_Plate2_H05


title: Sample 1532_PS_VE_Plate2_H02


title: Sample 1522_PS_VE_Plate2_G04


title: Sample 1530_PS_VE_Plate2_G12


title: Sample 1519_PS_VE_Plate2_G01


title: Sample 1531_PS_VE_Plate2_H01


title: Sample 1524_PS_VE_Plate2_G06


title: Sample 1521_PS_VE_Plate2_G03


title: Sample 1525_PS_VE_Plate2_G07


title: Sample 1520_PS_VE_Plate2_G02


title: Sample 1529_PS_VE_Plate2_G11


title: Sample 1528_PS_VE_Plate2_G10


title: Sample 1517_PS_VE_Plate2_F11


title: Sample 1534_PS_VE_Plate2_H04


title: Sample 1523_PS_VE_Plate2_G05


title: Sample 1516_PS_VE_Plate2_F10


title: Sample 1527_PS_VE_Plate2_G09


title: Sample 1533_PS_VE_Plate2_H03


title: Sample 1526_PS_VE_Plate2_G08


title: Sample 1544_PS_VE_Plate3_A02


title: Sample 1515_PS_VE_Plate2_F09


title: Sample 1611_PS_VE_Plate3_F09


title: Sample 1514_PS_VE_Plate2_F08


title: Sample 1512_PS_VE_Plate2_F06


title: Sample 1511_PS_VE_Plate2_F05


title: Sample 1510_PS_VE_Plate2_F04


title: Sample 1509_PS_VE_Plate2_F03


title: Sample 1518_PS_VE_Plate2_F12


title: Sample 1508_PS_VE_Plate2_F02


title: Sample 1507_PS_VE_Plate2_F01


title: Sample 1503_PS_VE_Plate2_E09


title: Sample 1500_PS_VE_Plate2_E06


title: Sample 1498_PS_VE_Plate2_E04


title: Sample 1495_PS_VE_Plate2_E01


title: Sample 1499_PS_VE_Plate2_E05


title: Sample 1494_PS_VE_Plate2_D12


title: Sample 1504_PS_VE_Plate2_E10


title: Sample 1501_PS_VE_Plate2_E07


title: Sample 1502_PS_VE_Plate2_E08


title: Sample 1505_PS_VE_Plate2_E11


title: Sample 1513_PS_VE_Plate2_F07


title: Sample 1486_PS_VE_Plate2_D04


title: Sample 1490_PS_VE_Plate2_D08


title: Sample 1485_PS_VE_Plate2_D03


title: Sample 1492_PS_VE_Plate2_D10


title: Sample 1491_PS_VE_Plate2_D09


title: Sample 1487_PS_VE_Plate2_D05


title: Sample 1488_PS_VE_Plate2_D06


title: Sample 1484_PS_VE_Plate2_D02


title: Sample 1482_PS_VE_Plate2_C12


title: Sample 1479_PS_VE_Plate2_C09


title: Sample 1496_PS_VE_Plate2_E02


title: Sample 1497_PS_VE_Plate2_E03


title: Sample 1480_PS_VE_Plate2_C10


title: Sample 1489_PS_VE_Plate2_D07


title: Sample 1506_PS_VE_Plate2_E12


title: Sample 1483_PS_VE_Plate2_D01


title: Sample 1481_PS_VE_Plate2_C11


title: Sample 1469_PS_VE_Plate2_B11


title: Sample 1471_PS_VE_Plate2_C01


title: Sample 1477_PS_VE_Plate2_C07


title: Sample 1468_PS_VE_Plate2_B10


title: Sample 1493_PS_VE_Plate2_D11


title: Sample 1466_PS_VE_Plate2_B08


title: Sample 1463_PS_VE_Plate2_B05


title: Sample 1473_PS_VE_Plate2_C03


title: Sample 1460_PS_VE_Plate2_B02


title: Sample 1474_PS_VE_Plate2_C04


title: Sample 1465_PS_VE_Plate2_B07


title: Sample 1461_PS_VE_Plate2_B03


title: Sample 1462_PS_VE_Plate2_B04


title: Sample 1467_PS_VE_Plate2_B09


title: Sample 1459_PS_VE_Plate2_B01


title: Sample 1475_PS_VE_Plate2_C05


title: Sample 1458_PS_VE_Plate2_A12


title: Sample 1457_PS_VE_Plate2_A11


title: Sample 1454_PS_VE_Plate2_A08


title: Sample 1472_PS_VE_Plate2_C02


title: Sample 1470_PS_VE_Plate2_B12


title: Sample 1455_PS_VE_Plate2_A09


title: Sample 1476_PS_VE_Plate2_C06


title: Sample 1456_PS_VE_Plate2_A10


title: Sample 1452_PS_VE_Plate2_A06


title: Sample 1451_PS_VE_Plate2_A05


title: Sample 1453_PS_VE_Plate2_A07


title: Sample 1464_PS_VE_Plate2_B06


title: Sample 1450_PS_VE_Plate2_A04


title: Sample 1446_PS_VE_Plate1_H12


title: Sample 1445_PS_VE_Plate1_H11


title: Sample 1444_PS_VE_Plate1_H10


title: Sample 1443_PS_VE_Plate1_H09


title: Sample 1438_PS_VE_Plate1_H04


title: Sample 1440_PS_VE_Plate1_H06


title: Sample 1439_PS_VE_Plate1_H05


title: Sample 1436_PS_VE_Plate1_H02


title: Sample 1437_PS_VE_Plate1_H03


title: Sample 1441_PS_VE_Plate1_H07


title: Sample 1434_PS_VE_Plate1_G12


title: Sample 1433_PS_VE_Plate1_G11


title: Sample 1431_PS_VE_Plate1_G09


title: Sample 1430_PS_VE_Plate1_G08


title: Sample 1432_PS_VE_Plate1_G10


title: Sample 1429_PS_VE_Plate1_G07


title: Sample 1449_PS_VE_Plate2_A03


title: Sample 1447_PS_VE_Plate2_A01


title: Sample 1448_PS_VE_Plate2_A02


title: Sample 1426_PS_VE_Plate1_G04


title: Sample 1425_PS_VE_Plate1_G03


title: Sample 1423_PS_VE_Plate1_G01


title: Sample 1424_PS_VE_Plate1_G02


title: Sample 1422_PS_VE_Plate1_F12


title: Sample 1428_PS_VE_Plate1_G06


title: Sample 1427_PS_VE_Plate1_G05


title: Sample 1442_PS_VE_Plate1_H08


title: Sample 1421_PS_VE_Plate1_F11


title: Sample 1415_PS_VE_Plate1_F05


title: Sample 1416_PS_VE_Plate1_F06


title: Sample 1418_PS_VE_Plate1_F08


title: Sample 1419_PS_VE_Plate1_F09


title: Sample 1413_PS_VE_Plate1_F03


title: Sample 1420_PS_VE_Plate1_F10


title: Sample 1412_PS_VE_Plate1_F02


title: Sample 1414_PS_VE_Plate1_F04


title: Sample 1417_PS_VE_Plate1_F07


title: Sample 1435_PS_VE_Plate1_H01


title: Sample 1407_PS_VE_Plate1_E09


title: Sample 1406_PS_VE_Plate1_E08


title: Sample 1408_PS_VE_Plate1_E10


title: Sample 1405_PS_VE_Plate1_E07


title: Sample 1403_PS_VE_Plate1_E05


title: Sample 1401_PS_VE_Plate1_E03


title: Sample 1397_PS_VE_Plate1_D11


title: Sample 1400_PS_VE_Plate1_E02


title: Sample 1399_PS_VE_Plate1_E01


title: Sample 1411_PS_VE_Plate1_F01


title: Sample 1395_PS_VE_Plate1_D09


title: Sample 1394_PS_VE_Plate1_D08


title: Sample 1404_PS_VE_Plate1_E06


title: Sample 1393_PS_VE_Plate1_D07


title: Sample 1388_PS_VE_Plate1_D02


title: Sample 1396_PS_VE_Plate1_D10


title: Sample 1392_PS_VE_Plate1_D06


title: Sample 1389_PS_VE_Plate1_D03


title: Sample 1387_PS_VE_Plate1_D01


title: Sample 1410_PS_VE_Plate1_E12


title: Sample 1409_PS_VE_Plate1_E11


title: Sample 1390_PS_VE_Plate1_D04


title: Sample 1402_PS_VE_Plate1_E04


title: Sample 1386_PS_VE_Plate1_C12


title: Sample 1384_PS_VE_Plate1_C10


title: Sample 1383_PS_VE_Plate1_C09


title: Sample 1381_PS_VE_Plate1_C07


title: Sample 1380_PS_VE_Plate1_C06


title: Sample 1398_PS_VE_Plate1_D12


title: Sample 1379_PS_VE_Plate1_C05


title: Sample 1382_PS_VE_Plate1_C08


title: Sample 1378_PS_VE_Plate1_C04


title: Sample 1377_PS_VE_Plate1_C03


title: Sample 1375_PS_VE_Plate1_C01


title: Sample 1376_PS_VE_Plate1_C02


title: Sample 1391_PS_VE_Plate1_D05


title: Sample 1374_PS_VE_Plate1_B12


title: Sample 1371_PS_VE_Plate1_B09


title: Sample 1373_PS_VE_Plate1_B11


title: Sample 1372_PS_VE_Plate1_B10


title: Sample 1385_PS_VE_Plate1_C11


title: Sample 1367_PS_VE_Plate1_B05


title: Sample 1370_PS_VE_Plate1_B08


title: Sample 1368_PS_VE_Plate1_B06


title: Sample 1363_PS_VE_Plate1_B01


title: Sample 1361_PS_VE_Plate1_A11


title: Sample 1357_PS_VE_Plate1_A07


title: Sample 1360_PS_VE_Plate1_A10


title: Sample 1365_PS_VE_Plate1_B03


title: Sample 1358_PS_VE_Plate1_A08


title: Sample 1364_PS_VE_Plate1_B02


title: Sample 1362_PS_VE_Plate1_A12


title: Sample 1366_PS_VE_Plate1_B04


title: Sample 1356_PS_VE_Plate1_A06


title: Sample 1359_PS_VE_Plate1_A09


title: Sample 1354_PS_VE_Plate1_A04


title: Sample 1355_PS_VE_Plate1_A05


title: Sample 1353_PS_VE_Plate1_A03


title: Sample 1351_PS_VE_Plate1_A01


title: Sample 1352_PS_VE_Plate1_A02


title: Sample 1350_PS_VE_Plate12_H12


title: Sample 1369_PS_VE_Plate1_B07


title: Sample 1349_PS_VE_Plate12_H11


title: Sample 1345_PS_VE_Plate12_H07


title: Sample 1344_PS_VE_Plate12_H06


title: Sample 1346_PS_VE_Plate12_H08


title: Sample 1348_PS_VE_Plate12_H10


title: Sample 1343_PS_VE_Plate12_H05


title: Sample 1341_PS_VE_Plate12_H03


title: Sample 1339_PS_VE_Plate12_H01


title: Sample 1342_PS_VE_Plate12_H04


title: Sample 1337_PS_VE_Plate12_G11


title: Sample 1340_PS_VE_Plate12_H02


title: Sample 1333_PS_VE_Plate12_G07


title: Sample 1338_PS_VE_Plate12_G12


title: Sample 1331_PS_VE_Plate12_G05


title: Sample 1336_PS_VE_Plate12_G10


title: Sample 1334_PS_VE_Plate12_G08


title: Sample 1330_PS_VE_Plate12_G04


title: Sample 1335_PS_VE_Plate12_G09


title: Sample 1347_PS_VE_Plate12_H09


title: Sample 1326_PS_VE_Plate12_F12


title: Sample 1327_PS_VE_Plate12_G01


title: Sample 1328_PS_VE_Plate12_G02


title: Sample 1325_PS_VE_Plate12_F11


title: Sample 1323_PS_VE_Plate12_F09


title: Sample 1324_PS_VE_Plate12_F10


title: Sample 1329_PS_VE_Plate12_G03


title: Sample 1322_PS_VE_Plate12_F08


title: Sample 1321_PS_VE_Plate12_F07


title: Sample 1319_PS_VE_Plate12_F05


title: Sample 1318_PS_VE_Plate12_F04


title: Sample 1320_PS_VE_Plate12_F06


title: Sample 1316_PS_VE_Plate12_F02


title: Sample 1315_PS_VE_Plate12_F01


title: Sample 1317_PS_VE_Plate12_F03


title: Sample 1478_PS_VE_Plate2_C08


title: Sample 1313_PS_VE_Plate12_E11


title: Sample 1309_PS_VE_Plate12_E07


title: Sample 1314_PS_VE_Plate12_E12


title: Sample 1308_PS_VE_Plate12_E06


title: Sample 1307_PS_VE_Plate12_E05


title: Sample 1305_PS_VE_Plate12_E03


title: Sample 1306_PS_VE_Plate12_E04


title: Sample 1303_PS_VE_Plate12_E01


title: Sample 1310_PS_VE_Plate12_E08


title: Sample 1304_PS_VE_Plate12_E02


title: Sample 1302_PS_VE_Plate12_D12


title: Sample 1301_PS_VE_Plate12_D11


title: Sample 1298_PS_VE_Plate12_D08


title: Sample 1297_PS_VE_Plate12_D07


title: Sample 1300_PS_VE_Plate12_D10


title: Sample 1296_PS_VE_Plate12_D06


title: Sample 1293_PS_VE_Plate12_D03


title: Sample 1294_PS_VE_Plate12_D04


title: Sample 1292_PS_VE_Plate12_D02


title: Sample 1291_PS_VE_Plate12_D01


title: Sample 1290_PS_VE_Plate12_C12


title: Sample 1288_PS_VE_Plate12_C10


title: Sample 1289_PS_VE_Plate12_C11


title: Sample 1311_PS_VE_Plate12_E09


title: Sample 1312_PS_VE_Plate12_E10


title: Sample 1287_PS_VE_Plate12_C09


title: Sample 1285_PS_VE_Plate12_C07


title: Sample 1284_PS_VE_Plate12_C06


title: Sample 1283_PS_VE_Plate12_C05


title: Sample 1282_PS_VE_Plate12_C04


title: Sample 1280_PS_VE_Plate12_C02


title: Sample 1281_PS_VE_Plate12_C03


title: Sample 1277_PS_VE_Plate12_B11


title: Sample 1278_PS_VE_Plate12_B12


title: Sample 1279_PS_VE_Plate12_C01


title: Sample 1295_PS_VE_Plate12_D05


title: Sample 1299_PS_VE_Plate12_D09


title: Sample 1276_PS_VE_Plate12_B10


title: Sample 1272_PS_VE_Plate12_B06


title: Sample 1275_PS_VE_Plate12_B09


title: Sample 1270_PS_VE_Plate12_B04


title: Sample 1271_PS_VE_Plate12_B05


title: Sample 1273_PS_VE_Plate12_B07


title: Sample 1274_PS_VE_Plate12_B08


title: Sample 1286_PS_VE_Plate12_C08


title: Sample 1268_PS_VE_Plate12_B02


title: Sample 1269_PS_VE_Plate12_B03


title: Sample 1266_PS_VE_Plate12_A12


title: Sample 1267_PS_VE_Plate12_B01


title: Sample 1265_PS_VE_Plate12_A11


title: Sample 1262_PS_VE_Plate12_A08


title: Sample 1263_PS_VE_Plate12_A09


title: Sample 1264_PS_VE_Plate12_A10


title: Sample 1261_PS_VE_Plate12_A07


title: Sample 1260_PS_VE_Plate12_A06


title: Sample 1259_PS_VE_Plate12_A05


title: Sample 1253_PS_VE_Plate11_H11


title: Sample 1251_PS_VE_Plate11_H09


title: Sample 1332_PS_VE_Plate12_G06


title: Sample 1256_PS_VE_Plate12_A02


title: Sample 1250_PS_VE_Plate11_H08


title: Sample 1255_PS_VE_Plate12_A01


title: Sample 1254_PS_VE_Plate11_H12


title: Sample 1257_PS_VE_Plate12_A03


title: Sample 1252_PS_VE_Plate11_H10


title: Sample 1245_PS_VE_Plate11_H03


title: Sample 1242_PS_VE_Plate11_G12


title: Sample 1244_PS_VE_Plate11_H02


title: Sample 1246_PS_VE_Plate11_H04


title: Sample 1249_PS_VE_Plate11_H07


title: Sample 1243_PS_VE_Plate11_H01


title: Sample 1241_PS_VE_Plate11_G11


title: Sample 1247_PS_VE_Plate11_H05


title: Sample 1240_PS_VE_Plate11_G10


title: Sample 1238_PS_VE_Plate11_G08


title: Sample 1235_PS_VE_Plate11_G05


title: Sample 1236_PS_VE_Plate11_G06


title: Sample 1239_PS_VE_Plate11_G09


title: Sample 1258_PS_VE_Plate12_A04


title: Sample 1232_PS_VE_Plate11_G02


title: Sample 1230_PS_VE_Plate11_F12


title: Sample 1233_PS_VE_Plate11_G03


title: Sample 1237_PS_VE_Plate11_G07


title: Sample 1229_PS_VE_Plate11_F11


title: Sample 1231_PS_VE_Plate11_G01


title: Sample 1224_PS_VE_Plate11_F06


title: Sample 1221_PS_VE_Plate11_F03


title: Sample 1223_PS_VE_Plate11_F05


title: Sample 1226_PS_VE_Plate11_F08


title: Sample 1228_PS_VE_Plate11_F10


title: Sample 1227_PS_VE_Plate11_F09


title: Sample 1225_PS_VE_Plate11_F07


title: Sample 1222_PS_VE_Plate11_F04


title: Sample 1216_PS_VE_Plate11_E10


title: Sample 1248_PS_VE_Plate11_H06


title: Sample 1214_PS_VE_Plate11_E08


title: Sample 1234_PS_VE_Plate11_G04


title: Sample 1217_PS_VE_Plate11_E11


title: Sample 1218_PS_VE_Plate11_E12


title: Sample 1215_PS_VE_Plate11_E09


title: Sample 1220_PS_VE_Plate11_F02


title: Sample 1209_PS_VE_Plate11_E03


title: Sample 1210_PS_VE_Plate11_E04


title: Sample 1207_PS_VE_Plate11_E01


title: Sample 1212_PS_VE_Plate11_E06


title: Sample 1208_PS_VE_Plate11_E02


title: Sample 1211_PS_VE_Plate11_E05


title: Sample 1213_PS_VE_Plate11_E07


title: Sample 1202_PS_VE_Plate11_D08


title: Sample 1204_PS_VE_Plate11_D10


title: Sample 1200_PS_VE_Plate11_D06


title: Sample 1206_PS_VE_Plate11_D12


title: Sample 1203_PS_VE_Plate11_D09


title: Sample 1196_PS_VE_Plate11_D02


title: Sample 1197_PS_VE_Plate11_D03


title: Sample 1201_PS_VE_Plate11_D07


title: Sample 1219_PS_VE_Plate11_F01


title: Sample 1195_PS_VE_Plate11_D01


title: Sample 1193_PS_VE_Plate11_C11


title: Sample 1199_PS_VE_Plate11_D05


title: Sample 1191_PS_VE_Plate11_C09


title: Sample 1188_PS_VE_Plate11_C06


title: Sample 1189_PS_VE_Plate11_C07


title: Sample 1187_PS_VE_Plate11_C05


title: Sample 1198_PS_VE_Plate11_D04


title: Sample 1186_PS_VE_Plate11_C04


title: Sample 1183_PS_VE_Plate11_C01


title: Sample 1205_PS_VE_Plate11_D11


title: Sample 1182_PS_VE_Plate11_B12


title: Sample 1180_PS_VE_Plate11_B10


title: Sample 1192_PS_VE_Plate11_C10


title: Sample 1194_PS_VE_Plate11_C12


title: Sample 1179_PS_VE_Plate11_B09


title: Sample 1178_PS_VE_Plate11_B08


title: Sample 1190_PS_VE_Plate11_C08


title: Sample 1174_PS_VE_Plate11_B04


title: Sample 1176_PS_VE_Plate11_B06


title: Sample 1173_PS_VE_Plate11_B03


title: Sample 1168_PS_VE_Plate11_A10


title: Sample 1172_PS_VE_Plate11_B02


title: Sample 1171_PS_VE_Plate11_B01


title: Sample 1169_PS_VE_Plate11_A11


title: Sample 1166_PS_VE_Plate11_A08


title: Sample 1165_PS_VE_Plate11_A07


title: Sample 1161_PS_VE_Plate11_A03


title: Sample 1177_PS_VE_Plate11_B07


title: Sample 1164_PS_VE_Plate11_A06


title: Sample 1184_PS_VE_Plate11_C02


title: Sample 1162_PS_VE_Plate11_A04


title: Sample 1163_PS_VE_Plate11_A05


title: Sample 1181_PS_VE_Plate11_B11


title: Sample 1157_PS_VE_Plate10_H11


title: Sample 1158_PS_VE_Plate10_H12


title: Sample 1154_PS_VE_Plate10_H08


title: Sample 1159_PS_VE_Plate11_A01


title: Sample 1175_PS_VE_Plate11_B05


title: Sample 1170_PS_VE_Plate11_A12


title: Sample 1151_PS_VE_Plate10_H05


title: Sample 1155_PS_VE_Plate10_H09


title: Sample 1153_PS_VE_Plate10_H07


title: Sample 1156_PS_VE_Plate10_H10


title: Sample 1150_PS_VE_Plate10_H04


title: Sample 1167_PS_VE_Plate11_A09


title: Sample 1145_PS_VE_Plate10_G11


title: Sample 1147_PS_VE_Plate10_H01


title: Sample 1146_PS_VE_Plate10_G12


title: Sample 1144_PS_VE_Plate10_G10


title: Sample 1160_PS_VE_Plate11_A02


title: Sample 1148_PS_VE_Plate10_H02


title: Sample 1142_PS_VE_Plate10_G08


title: Sample 1143_PS_VE_Plate10_G09


title: Sample 1137_PS_VE_Plate10_G03


title: Sample 1135_PS_VE_Plate10_G01


title: Sample 1152_PS_VE_Plate10_H06


title: Sample 1140_PS_VE_Plate10_G06


title: Sample 1139_PS_VE_Plate10_G05


title: Sample 1129_PS_VE_Plate10_F07


title: Sample 1138_PS_VE_Plate10_G04


title: Sample 1126_PS_VE_Plate10_F04


title: Sample 1127_PS_VE_Plate10_F05


title: Sample 1141_PS_VE_Plate10_G07


title: Sample 1119_PS_VE_Plate10_E09


title: Sample 1124_PS_VE_Plate10_F02


title: Sample 1136_PS_VE_Plate10_G02


title: Sample 1134_PS_VE_Plate10_F12


title: Sample 1133_PS_VE_Plate10_F11


title: Sample 1132_PS_VE_Plate10_F10


title: Sample 1131_PS_VE_Plate10_F09


title: Sample 1149_PS_VE_Plate10_H03


title: Sample 1113_PS_VE_Plate10_E03


title: Sample 1110_PS_VE_Plate10_D12


title: Sample 1114_PS_VE_Plate10_E04


title: Sample 1128_PS_VE_Plate10_F06


title: Sample 1125_PS_VE_Plate10_F03


title: Sample 1123_PS_VE_Plate10_F01


title: Sample 1130_PS_VE_Plate10_F08


title: Sample 1117_PS_VE_Plate10_E07


title: Sample 1122_PS_VE_Plate10_E12


title: Sample 1121_PS_VE_Plate10_E11


title: Sample 1118_PS_VE_Plate10_E08


title: Sample 1103_PS_VE_Plate10_D05


title: Sample 1101_PS_VE_Plate10_D03


title: Sample 1100_PS_VE_Plate10_D02


title: Sample 1116_PS_VE_Plate10_E06


title: Sample 1112_PS_VE_Plate10_E02


title: Sample 1097_PS_VE_Plate10_C11


title: Sample 1099_PS_VE_Plate10_D01


title: Sample 1108_PS_VE_Plate10_D10


title: Sample 1120_PS_VE_Plate10_E10


title: Sample 1109_PS_VE_Plate10_D11


title: Sample 1107_PS_VE_Plate10_D09


title: Sample 1105_PS_VE_Plate10_D07


title: Sample 1115_PS_VE_Plate10_E05


title: Sample 1106_PS_VE_Plate10_D08


title: Sample 1092_PS_VE_Plate10_C06


title: Sample 1104_PS_VE_Plate10_D06


title: Sample 1090_PS_VE_Plate10_C04


title: Sample 1102_PS_VE_Plate10_D04


title: Sample 1085_PS_VE_Plate10_B11


title: Sample 1098_PS_VE_Plate10_C12


title: Sample 1096_PS_VE_Plate10_C10


title: Sample 1111_PS_VE_Plate10_E01


title: Sample 1095_PS_VE_Plate10_C09


title: Sample 1093_PS_VE_Plate10_C07


title: Sample 1082_PS_VE_Plate10_B08


title: Sample 1089_PS_VE_Plate10_C03


title: Sample 1091_PS_VE_Plate10_C05


title: Sample 1081_PS_VE_Plate10_B07


title: Sample 1094_PS_VE_Plate10_C08


title: Sample 1086_PS_VE_Plate10_B12


title: Sample 1078_PS_VE_Plate10_B04


title: Sample 1075_PS_VE_Plate10_B01


title: Sample 1084_PS_VE_Plate10_B10


title: Sample 1087_PS_VE_Plate10_C01


title: Sample 1070_PS_VE_Plate10_A08


title: Sample 1072_PS_VE_Plate10_A10


title: Sample 1083_PS_VE_Plate10_B09


title: Sample 1068_PS_VE_Plate10_A06


title: Sample 1076_PS_VE_Plate10_B02


title: Sample 1080_PS_VE_Plate10_B06


title: Sample 1071_PS_VE_Plate10_A09


title: Sample 1077_PS_VE_Plate10_B03


title: Sample 1079_PS_VE_Plate10_B05


title: Sample 1073_PS_VE_Plate10_A11


title: Sample 1088_PS_VE_Plate10_C02


title: Sample 1069_PS_VE_Plate10_A07


title: Sample 1062_E7.5_Plate16_H12


title: Sample 1066_PS_VE_Plate10_A04


title: Sample 1065_PS_VE_Plate10_A03


title: Sample 1059_E7.5_Plate16_H09


title: Sample 1064_PS_VE_Plate10_A02


title: Sample 1074_PS_VE_Plate10_A12


title: Sample 1056_E7.5_Plate16_H06


title: Sample 1060_E7.5_Plate16_H10


title: Sample 1061_E7.5_Plate16_H11


title: Sample 1058_E7.5_Plate16_H08


title: Sample 1057_E7.5_Plate16_H07


title: Sample 1185_PS_VE_Plate11_C03


title: Sample 1054_E7.5_Plate16_H04


title: Sample 1055_E7.5_Plate16_H05


title: Sample 1052_E7.5_Plate16_H02


title: Sample 1053_E7.5_Plate16_H03


title: Sample 1051_E7.5_Plate16_H01


title: Sample 1048_E7.5_Plate16_G10


title: Sample 1049_E7.5_Plate16_G11


title: Sample 1067_PS_VE_Plate10_A05


title: Sample 1044_E7.5_Plate16_G06


title: Sample 1043_E7.5_Plate16_G05


title: Sample 1050_E7.5_Plate16_G12


title: Sample 1040_E7.5_Plate16_G02


title: Sample 1063_PS_VE_Plate10_A01


title: Sample 1046_E7.5_Plate16_G08


title: Sample 1041_E7.5_Plate16_G03


title: Sample 1039_E7.5_Plate16_G01


title: Sample 1042_E7.5_Plate16_G04


title: Sample 1038_E7.5_Plate16_F12


title: Sample 1037_E7.5_Plate16_F11


title: Sample 1033_E7.5_Plate16_F07


title: Sample 1034_E7.5_Plate16_F08


title: Sample 1036_E7.5_Plate16_F10


title: Sample 1029_E7.5_Plate16_F03


title: Sample 1035_E7.5_Plate16_F09


title: Sample 1031_E7.5_Plate16_F05


title: Sample 1030_E7.5_Plate16_F04


title: Sample 1032_E7.5_Plate16_F06


title: Sample 1025_E7.5_Plate16_E11


title: Sample 1027_E7.5_Plate16_F01


title: Sample 1024_E7.5_Plate16_E10


title: Sample 1028_E7.5_Plate16_F02


title: Sample 1026_E7.5_Plate16_E12


title: Sample 1045_E7.5_Plate16_G07


title: Sample 1023_E7.5_Plate16_E09


title: Sample 1019_E7.5_Plate16_E05


title: Sample 1021_E7.5_Plate16_E07


title: Sample 1020_E7.5_Plate16_E06


title: Sample 1017_E7.5_Plate16_E03


title: Sample 1047_E7.5_Plate16_G09


title: Sample 1013_E7.5_Plate16_D11


title: Sample 1015_E7.5_Plate16_E01


title: Sample 1022_E7.5_Plate16_E08


title: Sample 1009_E7.5_Plate16_D07


title: Sample 1016_E7.5_Plate16_E02


title: Sample 1014_E7.5_Plate16_D12


title: Sample 1010_E7.5_Plate16_D08


title: Sample 1011_E7.5_Plate16_D09


title: Sample 1012_E7.5_Plate16_D10


title: Sample 1018_E7.5_Plate16_E04


title: Sample 1000_E7.5_Plate16_C10


title: Sample 999_E7.5_Plate16_C09


title: Sample 998_E7.5_Plate16_C08


title: Sample 1007_E7.5_Plate16_D05


title: Sample 1006_E7.5_Plate16_D04


title: Sample 996_E7.5_Plate16_C06


title: Sample 995_E7.5_Plate16_C05


title: Sample 1001_E7.5_Plate16_C11


title: Sample 1004_E7.5_Plate16_D02


title: Sample 1005_E7.5_Plate16_D03


title: Sample 989_E7.5_Plate16_B11


title: Sample 1002_E7.5_Plate16_C12


title: Sample 1003_E7.5_Plate16_D01


title: Sample 994_E7.5_Plate16_C04


title: Sample 993_E7.5_Plate16_C03


title: Sample 992_E7.5_Plate16_C02


title: Sample 984_E7.5_Plate16_B06


title: Sample 990_E7.5_Plate16_B12


title: Sample 991_E7.5_Plate16_C01


title: Sample 981_E7.5_Plate16_B03


title: Sample 988_E7.5_Plate16_B10


title: Sample 997_E7.5_Plate16_C07


title: Sample 978_E7.5_Plate16_A12


title: Sample 987_E7.5_Plate16_B09


title: Sample 976_E7.5_Plate16_A10


title: Sample 986_E7.5_Plate16_B08


title: Sample 973_E7.5_Plate16_A07


title: Sample 985_E7.5_Plate16_B07


title: Sample 969_E7.5_Plate16_A03


title: Sample 980_E7.5_Plate16_B02


title: Sample 983_E7.5_Plate16_B05


title: Sample 979_E7.5_Plate16_B01


title: Sample 967_E7.5_Plate16_A01


title: Sample 964_E7.5_Plate15_H10


title: Sample 977_E7.5_Plate16_A11


title: Sample 975_E7.5_Plate16_A09


title: Sample 974_E7.5_Plate16_A08


title: Sample 972_E7.5_Plate16_A06


title: Sample 965_E7.5_Plate15_H11


title: Sample 970_E7.5_Plate16_A04


title: Sample 971_E7.5_Plate16_A05


title: Sample 959_E7.5_Plate15_H05


title: Sample 958_E7.5_Plate15_H04


title: Sample 982_E7.5_Plate16_B04


title: Sample 968_E7.5_Plate16_A02


title: Sample 966_E7.5_Plate15_H12


title: Sample 963_E7.5_Plate15_H09


title: Sample 953_E7.5_Plate15_G11


title: Sample 952_E7.5_Plate15_G10


title: Sample 951_E7.5_Plate15_G09


title: Sample 962_E7.5_Plate15_H08


title: Sample 955_E7.5_Plate15_H01


title: Sample 957_E7.5_Plate15_H03


title: Sample 950_E7.5_Plate15_G08


title: Sample 945_E7.5_Plate15_G03


title: Sample 960_E7.5_Plate15_H06


title: Sample 948_E7.5_Plate15_G06


title: Sample 946_E7.5_Plate15_G04


title: Sample 961_E7.5_Plate15_H07


title: Sample 942_E7.5_Plate15_F12


title: Sample 940_E7.5_Plate15_F10


title: Sample 941_E7.5_Plate15_F11


title: Sample 939_E7.5_Plate15_F09