title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H10_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H11_AD012

status: Public on Sep 09 2019

type: SRA

city: La Jolla

first: Joseph

middle: R

last: Ecker

state: CA

address: 10010 North Torrey Pines Road

department: Genomic Analysis Laboratory

country: USA

institute: HHMI-Salk-Institute

library_source: genomic

data_processing:
Library strategy: single-nucleus methyl-3C sequencing (sn-m3C)
Raw reads were trimmed first 25bp and last 3bp of both read1 and read2 to remove random primer sequence and adaptase low complexity tails. For the alignment, Bismark is used. C to T converted and G to A converted reference genomes are prepared for hg19 using bismark_genome_preparation. Each read end is mapped separately using Bismark with Bowtie1 with read1 as complementary (always G to A converted) and read2 (always C to T converted) as original strand. After first alignment, unmapped reads are retained and splitted into 3 pieces by 40bp, 32bp, and 40bp after removing 5bp of both ends results in having 6 reads (read1 and read2). Six reads derived from unmapped reads are mapped separately using Bismark Bowtie1. All aligned reads are merged into BAM using picard that is query name sorted. The fragments with all the mapped reads aligned to the same positions are considered as duplicates and removed and DNA methylation tracks are generated. For each fragment, the outermost aligned reads are chosen for the chromatin conformation map generation.
Genome_build: hg19
Supplementary_files_format_and_content: tab delimited text files of methylcytosine calls; columns in allc files are: column 1 - chromosome; column 2 - position; column 3 - strand; column 4 - class; column 5 - mC reads; column 6 - total reads; column 7 - methylated (Boolean value indicating the result of statistical test for methylated cytosines)
tab delimited text files of contact.txt files; columns in contact.txt files are: column 1 - Sample_ID; column 2- chromosome_1; column 3 - Position_1; column 4 - Chromosome_2 ; column5 - Position_2; column6 - Strand_1; column 7 - Strand_2

molecule: genomic DNA

organism: Homo sapiens

source_name: Human frontal cortex

growth_protocol: Postmortem human brain biospecimens were obtained from brain donors of NIH NeuroBioBank and University of Maryland Brain and Tissue Bank.

extract_protocol: in situ Hi-C was performed as previously described using the NlaIII restriction enzyme. in situ 3C experiments were performed based on the previously described in situ Hi-C protocol with minor modifications. Briefly, prior to fixation, adherent cells were trypsinized, counted, and collected by centrifugation, and suspension cells were counted and collected by centrifugation. Cells were resuspended in culture media at a concentration of 1x106 cells per mL of media and fixed in 1% formaldehyde for 10 minutes at room temperature with shaking. For standard species mixture experiments, equal numbers of mouse and human cells were combined into a single tube prior to fixation. For the 1:10 dilution species mixture experiment, cells were resuspended at a concentration of 1x105 cells per mL of media prior to fixation. For the species mixture experiments where samples were mixed after fixation, each cell type was fixed independently as described above and combined at later stages in the protocol. in situ Hi-C samples were digested with the MboI restriction enzyme and processed as described previously. For in situ 3C experiments, samples were digested with DpnII enzyme overnight at 37ºC with gentle mixing. The following day, the sample was incubated at 62ºC for 10 minutes to inactivate the restriction enzyme. The typical biotin fill in step in the Hi-C protocol is omitted. The sample is then ligated for 4 hours at room temperature with T4 DNA ligase in the same manner as in in situ Hi-C experiments. The sample is then stained with Hoechst (0.1µg/µL) for the final 30 minutes of the ligation step. The sample is then passed through a 40 µM nylon cell strainer (Corning 431750) into a FACS tube prior to sorting. As a quality control step, 10% of the sample is taken for conventional library preparation and sequenced using shallow sequencing on a MiSeq. Libraries for bulk and single-cell methylomes were generated using snmC-seq2. A detailed step-by-step bench protocol for snmC-seq2 is provided as Supplement Methods in Luo et al. (2018) 23. Bulk methylome libraries were prepared manually using individual tubes. Single-cell methylome libraries were prepared using a Tecan Evo 100 robotic platform as described in Luo et al. (2018) 23. Sequencing libraries were sequenced using Illumina MiSeq and HiSeq 4000 instruments in PE150 mode.

tag: tissue

value: frontal cortex

tag: age

tag: Sex

value: Male

tag: donor


title: 190305_snm3Cseq_hs_29yr_BA10_UMB5580_1_UMB5580_2_A12_AD010


title: 190305_snm3Cseq_hs_29yr_BA10_UMB5580_1_UMB5580_2_A12_AD008


title: 190305_snm3Cseq_hs_29yr_BA10_UMB5580_1_UMB5580_2_A12_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H10_AD002


title: 190305_snm3Cseq_hs_29yr_BA10_UMB5580_1_UMB5580_2_A12_AD004


title: 190305_snm3Cseq_hs_29yr_BA10_UMB5580_1_UMB5580_2_A12_AD006


title: 190305_snm3Cseq_hs_29yr_BA10_UMB5580_1_UMB5580_2_A12_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H10_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G9_AD007


title: 190305_snm3Cseq_hs_29yr_BA10_UMB5580_1_UMB5580_2_A12_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G9_AD010


title: 190305_snm3Cseq_hs_29yr_BA10_UMB5580_1_UMB5580_2_A11_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G9_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G9_AD002


title: 190305_snm3Cseq_hs_29yr_BA10_UMB5580_1_UMB5580_2_A11_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G9_AD001


title: 190305_snm3Cseq_hs_29yr_BA10_UMB5580_1_UMB5580_2_A11_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G8_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G8_AD012


title: 190305_snm3Cseq_hs_29yr_BA10_UMB5580_1_UMB5580_2_A10_AD007


title: 190305_snm3Cseq_hs_29yr_BA10_UMB5580_1_UMB5580_2_A10_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G8_AD007


title: 190305_snm3Cseq_hs_29yr_BA10_UMB5580_1_UMB5580_2_A10_AD012


title: 190305_snm3Cseq_hs_29yr_BA10_UMB5580_1_UMB5580_2_A11_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H10_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G8_AD006


title: 190305_snm3Cseq_hs_29yr_BA10_UMB5580_1_UMB5580_2_A10_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G9_AD012


title: 190305_snm3Cseq_hs_29yr_BA10_UMB5580_1_UMB5580_2_A10_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G7_AD012


title: 190305_snm3Cseq_hs_29yr_BA10_UMB5580_1_UMB5580_2_A10_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G7_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G8_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H9_AD010


title: 190305_snm3Cseq_hs_29yr_BA10_UMB5580_1_UMB5580_2_A11_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G9_AD006


title: 190305_snm3Cseq_hs_29yr_BA10_UMB5580_1_UMB5580_2_B2_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G7_AD006


title: 190305_snm3Cseq_hs_29yr_BA10_UMB5580_1_UMB5580_2_B10_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G7_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H9_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H9_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H9_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G6_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G6_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H8_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H9_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G7_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H8_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G6_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G6_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G6_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H8_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H8_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H7_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H7_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G7_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H9_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H7_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H7_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H9_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G5_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G6_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H8_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G5_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G5_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G5_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G4_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H6_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H8_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G4_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H6_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H6_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H6_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G6_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G5_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G4_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H6_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H6_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G4_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G4_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G4_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G3_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H5_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H6_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H5_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H5_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G3_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G3_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G3_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H5_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H5_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H4_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G3_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G4_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G2_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G2_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H4_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G2_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H4_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H4_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G2_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G2_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G2_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H4_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H3_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H3_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H3_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G3_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H3_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G2_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G1_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G2_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G1_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G1_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G1_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H2_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H2_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H2_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G1_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G1_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G1_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H2_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H4_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G1_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G3_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H2_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G12_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H1_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G12_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H1_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H4_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H1_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G12_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H1_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G12_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G12_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H12_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H12_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H12_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H12_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G11_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H2_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H12_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G11_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G11_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H12_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G11_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G11_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H12_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G11_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G11_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H1_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H11_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H12_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G11_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G10_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G10_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G12_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H11_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G10_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H11_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G10_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H11_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H11_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G10_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G10_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H11_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F9_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G10_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H11_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F9_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H10_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F9_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F9_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H11_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F9_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H10_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F9_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F9_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H10_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H10_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F9_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F8_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G9_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G9_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G9_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F8_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F8_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G9_AD004


title: 190305_snm3Cseq_hs_29yr_BA10_UMB5580_1_UMB5580_2_A3_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H10_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G9_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F8_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G8_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F8_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G8_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F8_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F7_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G8_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G8_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F7_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G8_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G9_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F7_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G7_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F7_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F7_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G8_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G7_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G7_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F7_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F6_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G7_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F6_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F8_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F6_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F6_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F6_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G7_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F6_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G7_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G6_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G6_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G6_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F5_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G6_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F5_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F5_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G6_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G6_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G5_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F5_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G5_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F5_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G5_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G5_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G5_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F4_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H10_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G7_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F4_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G5_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F5_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F5_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F4_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F4_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G4_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G4_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G4_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F3_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F3_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G4_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F3_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G4_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F5_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G4_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G4_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F3_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F3_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F3_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G4_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F4_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_H5_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G7_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F3_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G3_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G3_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F3_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G3_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F2_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G3_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G2_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F2_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F2_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G2_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G2_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F2_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F2_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F1_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F1_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F1_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G2_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G2_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F1_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F2_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G3_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F1_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G2_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G1_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F2_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F1_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G1_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F1_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G1_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G12_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G1_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F12_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G12_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G12_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G12_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F12_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F12_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G1_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G12_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F12_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F12_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G12_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F11_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G11_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G11_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F11_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F11_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F11_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G11_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G11_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G11_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F12_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F11_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F12_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G10_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F11_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F11_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G10_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G10_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F10_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G10_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F10_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G10_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G12_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G10_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F10_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F10_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G11_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F9_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F11_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F9_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F9_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F10_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E9_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E9_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E9_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E9_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F9_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F10_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E9_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E9_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E9_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E8_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G10_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F8_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F8_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F8_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F8_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E8_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F9_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F9_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E8_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F8_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E8_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F9_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F7_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F7_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E8_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E7_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F7_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E7_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F8_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E9_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E7_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F7_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F7_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F7_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E7_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F6_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E7_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F7_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F6_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E7_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E6_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E8_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F6_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F6_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F6_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F6_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E6_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E6_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E6_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F5_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F5_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F5_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F5_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F5_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E5_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E5_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E5_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E5_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E5_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F4_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F5_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F4_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F4_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E5_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E6_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E4_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E4_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E4_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F4_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F4_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F3_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_G5_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F4_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E4_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E4_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E4_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F3_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F3_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E3_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E4_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E3_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F3_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E3_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F3_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E3_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F2_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F2_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F3_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E2_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E3_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E3_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F2_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F2_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E2_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F1_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E2_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E2_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F1_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F1_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F1_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E2_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E2_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F1_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E2_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F2_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F1_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E3_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F12_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F12_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E1_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E1_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F12_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F12_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F12_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E12_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F12_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F11_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E1_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F12_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E1_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F11_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E1_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F11_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E12_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F11_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E12_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E12_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F11_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E1_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E1_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E12_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E12_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F10_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E11_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F10_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F10_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F10_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E11_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F10_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E11_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E12_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E11_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F10_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E9_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E11_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E11_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E9_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E10_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E10_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E9_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E9_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E10_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E9_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E10_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E9_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E10_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E10_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E8_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E9_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E11_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D9_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E8_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D9_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D9_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D9_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D9_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E8_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E8_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F10_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E7_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E7_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D9_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D8_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D8_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D8_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D9_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E8_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D8_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E7_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D8_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D8_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E7_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E7_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D8_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E9_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E8_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E8_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E6_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D7_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D7_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D7_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E7_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E6_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E5_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E6_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D7_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D7_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E5_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D7_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E6_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E5_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D6_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D7_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E6_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E6_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D5_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E4_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E5_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E3_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D6_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D5_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D6_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E5_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E4_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E4_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D6_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D5_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D6_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E4_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D6_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E4_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_F12_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D5_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E5_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D5_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E3_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E3_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E3_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E2_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D4_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D5_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E3_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D4_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E2_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E2_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D5_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D4_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D4_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E3_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D4_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E3_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D3_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D3_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E1_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E2_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D4_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D4_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E1_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E2_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D3_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D3_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D3_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E2_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E2_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E12_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D3_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E1_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E12_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D3_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E1_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D3_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E1_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E12_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D2_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E11_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D2_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E12_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D2_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D2_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E12_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D2_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E1_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D1_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E12_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D12_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E10_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D1_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E10_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D2_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D12_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E11_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E12_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D6_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D1_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D12_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E10_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E10_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D1_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D1_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E11_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D12_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E11_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D1_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E11_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D11_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D9_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E7_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D11_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D9_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D9_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E10_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D10_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D8_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D8_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D12_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D7_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D12_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D9_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D10_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D11_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D9_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C9_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D10_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D7_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D11_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D11_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D9_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D7_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D10_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_E10_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D9_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D8_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D10_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D10_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D10_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D6_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D7_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D6_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D9_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D10_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D7_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C9_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D7_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C9_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C9_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C9_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D7_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C9_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D7_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D5_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C9_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C7_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D6_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D6_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C7_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C8_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C8_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D7_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D5_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C8_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C7_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C8_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D5_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C7_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C8_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D6_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D5_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D4_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D5_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D5_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C7_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C7_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D4_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D4_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C6_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C6_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D6_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C5_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D4_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C6_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D5_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D3_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C6_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C6_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D4_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C6_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C5_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D4_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C5_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C5_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D3_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D3_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C5_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C4_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D5_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C5_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C5_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D3_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C4_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D2_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C3_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D2_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D3_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D3_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C4_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D2_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D3_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C4_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C4_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C3_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D1_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D1_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C4_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D2_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D2_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D2_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D1_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C3_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C2_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C3_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C3_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D4_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D1_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D1_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C2_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C3_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C2_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C2_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C2_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D1_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C2_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D12_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D1_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D12_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D12_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D12_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D12_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C1_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D12_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C1_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C1_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D12_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C1_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C2_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C1_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D11_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D11_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C1_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C1_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D11_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D11_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C12_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D11_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C12_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D11_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C12_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D12_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D10_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D11_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D10_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C12_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D10_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C12_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C11_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C12_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D10_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C11_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C11_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D10_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C2_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_D10_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C9_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C9_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C12_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C9_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C10_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C11_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C9_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C11_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C10_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C10_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C9_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C10_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C11_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C10_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C11_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C9_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C9_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B9_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C8_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B9_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C8_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C8_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C9_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C8_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C8_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B9_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B9_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B8_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C7_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C7_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C7_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C7_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B8_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B8_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B8_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C8_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B8_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C7_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B9_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B7_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C7_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C7_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C6_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B8_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B7_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C6_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B7_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C6_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C6_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B7_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B7_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C5_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B6_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C6_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C12_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C5_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B6_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C5_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C5_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C5_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B8_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B6_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B7_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C5_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C6_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B5_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C4_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B6_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C6_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B6_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B5_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C5_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B4_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C4_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B4_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C3_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C5_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B5_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C4_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B5_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B7_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B4_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B5_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C3_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B5_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B6_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B4_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C4_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B4_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C3_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C4_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B4_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C3_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B4_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C3_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C3_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B3_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B3_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C3_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B3_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C2_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B3_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C2_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C3_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B3_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C2_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C2_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B3_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C2_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B3_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C2_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C1_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B2_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C1_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C1_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C1_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C1_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B2_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C12_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B2_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B2_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B2_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C12_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B2_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B1_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C12_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C12_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C12_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B1_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C12_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B1_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B1_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C12_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B1_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B3_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C12_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B2_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C1_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C11_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B1_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B1_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C11_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C11_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B12_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B12_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C11_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B12_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C11_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B12_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C11_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B11_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C10_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C10_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B11_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C10_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C10_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C11_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C10_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B11_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C10_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B11_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_C10_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B9_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B12_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B12_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B9_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B11_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B10_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B9_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B10_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B8_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B9_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B10_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B11_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B11_AD006


title: Mi-343

city: Leipzig

first: Olga

last: Bondareva

state: Saxony

address: Philipp-Rosenthal-Straße 27

department: HI-MAG

country: Germany

institute: Helmholtz Zentrum München

description: bulk RNA

library_source: transcriptomic

data_processing:
Raw sequencing data for both single cell and bulk experiments were controlled for quality and trimmed.
For scRNA-seq paired end reads were aligned to the transcriptome using bwa (version 0.6.2-r126) with default parameters. The transcriptome contained all RefSeq gene models based on the mouse genome release mm10 downloaded from the UCSC genome browser.
The right mate of each read pair was mapped to the ensemble of all gene loci and to the set of 92 ERCC spike-ins in sense direction (Baker et al., 2005). Reads mapping to multiple loci were discarded.
The left read contains the barcode information: the first six bases corresponded to the cell specific barcode followed by six bases representing the unique molecular identifier (UMI). The remainder of the left read contains a polyT stretch. The left read was not used for mapping to the reference transcriptome.
For each cell barcode, the number of UMIs per transcript were counted and aggregated across all transcripts derived from the same gene locus.
The file GEO_info_cellbarcodes_cellid.txt (available on the series record) contains the cell barcode ID, which is also the processed data column header, cell barcode and cell markers. The same barcodes and IDs were used for each count*P.csv  file.
Based on binomial statistics, the number of observed UMIs were converted into transcript counts (Grün et al., 2014).
Bulk RNA-seq data were mapped to the reference mm10 genome using STAR (Dobin et al., 2013).
The identity of reads was identified through FeatureCount (Liao et al., 2014).
For scRNA-seq analyses, the RNA expression matrices from each cell were merged and further analysed with RaceID3 software package (Herman et al., 2018).
For both scRNA-seq and the bulk Brain-FACS RNA-seq analyses, differentially expressed genes between the cell types or cell clusters were identified using DESeq2 (Love et al., 2014).
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited and csv text files include count matrices for scRNA-seq data and bulk RNA-seq samples

molecule: total RNA

organism: Mus musculus

source_name: E14.5 brain

extract_protocol:
Mouse E14.5 brain was dissociated and either mCEL-Seq2 protocol for scRNA-seq or population-based FACS-sorting and bulk RNA-seq was performed.
scRNA-seq libraries were prepared according to mCEL-Seq2 protocol. Libraries were sequenced on an Illumina HiSeq 3000 sequencing system (pair-end multiplexing run, high output mode) at a depth of ~200,000 reads per cell. Population-based FACS-sorting and bulk RNA-seq  from neural cells was isolated using the Qiagen mini kit (#74104), while RNA from mural cells, endothelial cells and microglia was extracted using the Qiagen miRNeasy Micro kit (#1071023). Libraries for RNA-seq of the neural population were prepared using the Illumina TruSeq library preparation kit. For mural cells, endothelial cells and microglia, cDNA was prepared from isolated RNA using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech 634891). Libraries were prepared using the Nextera XT DNA library preparation kit (Illumina FC-131-1096) and sequenced on the HiSeq 2500 instrument.

tag: cell type

value: microglial

value: brain

tag: developmental stage


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B11_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B8_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B8_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B10_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B8_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B10_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B10_AD002


title: Ec-346

description: bulk RNA

value: endothelial


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B7_AD012


title: Ec-343

description: bulk RNA


title: Mi-346

description: bulk RNA


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B8_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B10_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B8_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B7_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B7_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B8_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B1_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B7_AD004


title: Ec-340

description: bulk RNA


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B7_AD002


title: Mu-346

description: bulk RNA

value: mural


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A9_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A9_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B6_AD012


title: Mi-340

description: bulk RNA


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B6_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B6_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A9_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B6_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A9_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B7_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A9_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A9_AD002


title: N-343

description: bulk RNA

value: neural


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B6_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B6_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B5_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B5_AD007


title: N-340

description: bulk RNA


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A8_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B5_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B5_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B5_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A8_AD012


title: Input_E10.5

city: Philadelphia

first: Douglas

middle: J

last: Epstein

state: PA

address: 415 Curie Boulevard

institute: University of Pennsylvania

data_processing:
Reads were aligned to mm9 genome using Bpwtie (for ATAC-seq and ChIP-seq) or RUM (for RNA-seq)
Peaks were called for ATAC-seq and ChIP-seq using either MACS or HOMER
Genome_build: mm9
Supplementary_files_format_and_content: Smoecko_raw_data_GEO.txt: column G-N (normalized reads); O-V (raw reads)
Supplementary_files_format_and_content: ShhP1_raw_data_GEO.txt: column G-M (normalized reads); N-T (raw reads)

source_name: Input E10.5

extract_protocol: Tissues were sonicated to extract and fragment the DNA. ChIP-seq was performed using Gli2 antibody (R&D AF3526)

tag: strain

value: Swiss Webster

value: head


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A8_AD007


title: Mu-340

description: bulk RNA


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B6_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A9_AD001


title: ATAC-seq_E11.5_lib5

source_name: Inner ear E11.5 wild type

extract_protocol: Libraries were prepared using the Nextera DNA Library Preparation kit (Illumina#15028211)

value: inner ear


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B6_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B4_AD010


title: Mu-343

description: bulk RNA


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A8_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B4_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B4_AD012


title: ATAC-seq_E11.5_lib4


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B4_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A8_AD004


title: Gli2_ChIP_seq_E10.5_head

source_name: Head E10.5


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B5_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B3_AD010


title: ATAC-seq_E11.5_lib3


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B3_AD008


title: ATAC-seq_E11.5_lib2


title: ATAC-seq_E11.5_lib1


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B3_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B3_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A7_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B4_AD006


title: RNA_seq_P1_ctrl_lib3

source_name: Inner ear E11.5 Shh_P1 sibling ctrl

extract_protocol: Total RNA was extracted using Rneasy Mini Kit (Qiagen,Cat#74104). Hi-seq library was prepared using NEBnext Ultra RNA Library Kit for Illumina (#E7530S)

tag: genotype


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B3_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A7_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B3_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A7_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B2_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B2_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B2_AD012


title: RNA_seq_P1_ctrl_lib2


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B2_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A7_AD007


title: RNA_seq_P1_ctrl_lib1


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B2_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B2_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B2_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A7_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A7_AD004


title: RNA_seq_P1_mutant_lib3

source_name: Inner ear E11.5 Shh_P1 mutant


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B2_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B1_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A7_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B1_AD010


title: RNA_seq_P1_mutant_lib2


title: RNA_seq_P1_mutant_lib1


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A7_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B1_AD006


title: RNA_seq_P1_mutant_lib4


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B1_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A6_AD012


title: RNA_seq_Smoecko_ctrl_lib4

source_name: Inner ear E11.5 Smoecko sibling ctrl


title: RNA_seq_Smoecko_ctrl_lib3


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A6_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B12_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B12_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B12_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A6_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B12_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A6_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B12_AD004


title: RNA_seq_Smoecko_ctrl_lib2


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B11_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B12_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B12_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A6_AD006


title: RNA_seq_Smoecko_ctrl_lib1


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B12_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B1_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B11_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B1_AD002


title: RNA_seq_Smoecko_mutant_lib4

source_name: Inner ear E11.5 Smoecko mutant


title: RNA_seq_Smoecko_mutant_lib2


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B11_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B11_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B11_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B11_AD007


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A6_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A6_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B11_AD002


title: RNA_seq_Smoecko_mutant_lib1


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A5_AD012


title: Ribo_SEQ_EPI-HuR-KO-2

city: Singapore

first: Lei

last: Sun

address: 8 College Road

country: Singapore

institute: Duke-NUS

description:
HuR master_Background.xlsx
EPI-HuR-KO-2_RPF

data_processing:
Adapter trimming with Cutadapt 1.8.3
Build transcriptome with Tophat 2.0.9
Select most abundant transcript with python script
To most abundant transcript dataset, allowing no mismatches and one map per read, using bowtie 1.0.0
Calculate RPKM within coding sequence
We have removed the genes that showed low expression (RPKM <1), leaving ~8.5k genes
Genome_build: Refseq 70
Supplementary_files_format_and_content: RPKMs of genes

source_name: Epididymal

growth_protocol: HuRflox/flox mice were originally purchased from the Jackson Laboratory and subsequently bred in house. HuRflox/floxmice were backcrossed with C57BL/6 mice for 2 generations and then used to cross adiponectin-Cre mice to generate AdipoQ-Cre;HuRf/+ mice. Heterozygous AdipoQ-Cre;HuRf/+ were then crossed with HuRf/f  to produce homozygous AdipoQ-Cre;HuRf/f. AdipoQ-Cre;HuRf/f were further bred with HuRf/f mice to generate AdipoQ-Cre;HuRf/f (HuR-FKO) and littermate HuRf/f controls (Ctrl) for experiments. All mice were kept under specific pathogen–free conditions in the animal vivarium at DUKE-NUS Medical School. All animal experimental protocols were approved by the Singapore SingHealth Research Facilities Institutional Animal Care and Use Committee.

extract_protocol:
Qiagen miRNeasy Mini Kit
NEBNext® Ultra™ RNA Library Prep Kit, NEBNext® Small RNA Library Prep Set for Illumina®

treatment_protocol:
male mice were sacrificed at 6-weeks old to harvest epididymal white adipose tissue (WAT). Tissues from 3 mice were pooled together as one replicate. Tissues were minced into small pieces and separated into 2 shares for RNA-seq and ribosome profiling. For RNA-seq, total RNAs were extracted with miRNeasy
For ribosome profiling, adipose tissue were homogenized with pestle and mortar in the presence of liquid nitrogen. The homogenized powder was transferred into 1.5 ml tubes and lysed with 1 ml lysis buffer (1% NP-40, 200 mM KOAc, 25 mM K-HEPES pH 7.2, 14 mM MgCl, 4 mM CaCl2). Samples were incubated on ice for 5 minutes and centrifuged at 9,000 g in a benchtop centrifuge at 4 °C for 10 minutes. The fat layer on top was removed and the 300 ul supernatant was collected. Following steps were conducted according to a published protocol from Reid, D. W., Shenolikar, S. & Nicchitta, C. V. Methods 91, 69–74, doi:10.1016/j.ymeth.2015.07.003 (2015).

value: huR knockout

value: epididymal white fat

tag: rip antibody

value: HuR antibody (Santa Cruz)


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B11_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B10_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B10_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B10_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B10_AD004


title: Ribo_SEQ_EPI-WT2

description:
HuR master_Background.xlsx
EPI-WT2_RPF

value: control


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B10_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A5_AD010


title: Ribo_SEQ_EPI-WT1

description:
HuR master_Background.xlsx
EPI-WT1_RPF


title: RNA_seq_Smoecko_mutant_lib3


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A5_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A5_AD006


title: RNA-SEQ_EPI-WT2

description:
HuR master_Background.xlsx
rna-EP12


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A5_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A5_AD004


title: RNA-SEQ_EPI-WT1

description:
HuR master_Background.xlsx
rna-EPI1


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A9_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A4_AD012


title: Ribo-SEQ_EPI-HuR-KO-1

description:
HuR master_Background.xlsx
EPI-HuR-KO-1_RPF


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A9_AD008


title: RNA-SEQ_EPI-HuR-KO-2

description:
HuR master_Background.xlsx
rna-EPI-HuRKO2


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A4_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_B10_AD001


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A9_AD007


title: RNA-SEQ_EPI-HuR-KO-1

description:
HuR master_Background.xlsx
rna-EPI-huRKO1


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A9_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A9_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A9_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A6_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A8_AD010


title: ATAC_DMSO_rep2_J14-2

city: New York

first: Richard

last: Koche

state: New York

institute: Memorial Sloan Kettering Cancer Center

data_processing:
ATAC-seq reads were filtered for quality and 3' trimmed for adapter sequences.
Reads were aligned to mouse assembly mm9 using bowtie2 (v2.2.2) with default parameters.
Read count tables were created using featureCounts v1.6.0.
To create density files, the reads were extended to 200 bp, the signal was then normalized to ten million mapped reads and converted to BigWig using BEDTools.
Genome_build: mm9
Supplementary_files_format_and_content: BigWig files containing read densities normalized to 10 million reads

source_name: KPsh

growth_protocol: 1e6 KP-sh cells were washed with PBS and plated on and off dox 8 days (-D8) before collecting cells for ATAC-SEQ analysis.  Cells maintained on dox and off dox were passaged every 48 hours.  At –D3 4mM dimethyl-alpha ketoglutarate (DM-aKG) was added to cells grown on dox, while DMSO was added to cells maintained on dox and cells maintained off dox.  All cell groups (ON DOX-DMSO, ON DOX-DM-aKG, OFF DOX-DMSO) were split into 6 well plates for replicate collection on –D2 in fresh media containing dox, DM-aKG, and DMSO as indicated.  This media was refreshed 24 hours before collection (-D1). On D0 cells were washed with PBS, trypsinized, and collected as a single cell suspension in complete DMEM.  50000 cells of each group were sorted using a BD-FACS-ARIA and after centrifugation and washing with PBS cell pellets were subjected to ATAC-SEQ lysis, transposition, and library preparation.

extract_protocol:
Cell nuclei were fresh prepared from 50,000 cells as previously described (Buenrostro et al. 2013 Nature Methods).
ATAC-seq was performed as previously described (Buenrostro et al. 2013 Nature Methods). For each sample, cell nuclei were prepared from 50,000 cells, and incubated with 2.5 uL transposase (Illumina) in a 50 uL reaction for 30 min at 37°C. Following purification of transposase fragmented DNA, the library was amplified by PCR and subjected to high-throughput sequencing using Illumina HiSeq 2500 platform.

treatment_protocol: On and off doxycycline, as described in growth conditions above.

value: PDAC cell line

tag: cell line

value: KPsh

tag: treatment

value: doxycycline


title: ATAC_p53on_rep1_J15-1


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A4_AD007

value: frontal cortex


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A4_AD006

value: Male


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A4_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A8_AD008


title: ATAC_DMSO_rep1_J14-1


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A4_AD002


title: ATAC_p53on_rep2_J15-2


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A8_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A3_AD010


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A8_AD001


title: ATAC_aKG_rep1_J13-1

value: aKG + doxycycline


title: HDF_MBD

city: Incheon Metropolitan City

first: Yong Seung

last: Lee

address: SimGokro 100gil 25

department: College of Medicine

country: South Korea

institute: Catholic Kwandong University

data_processing:
Illumina bcl2fastq v1.8.4 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence and masked for low-complexity or low-quality sequence with Trimmomatic0.32.
To estimate methylation levels, the MBD Seq reads were mapped to the genome of Homo sapiens(hg19) using Bowtie 1.1.1.
PCR duplicates were removed with Picard Mark Duplicates (version 1.118).
Raw data (the reads for mapped in genome) were normalized by RMS (Relative Methylayion Score) each ROI region using MEDIPS package (version 1.16.0).
Genome_build: UCSC hg19
Supplementary_files_format_and_content: tab-delimited text files include RMS values for each Sample and ROI. CGI_PROMOTER.rms.txt contains a relative methylation score matrix.

source_name: dermal fibroblast cells

growth_protocol: x/Entr cells were cultured with ultrasound treated hES culture medium include DMEM/F12, 20% serum replacement, 1 mM glutamine, 0.1% NEAA, 0.1% penicillin/streptomycin, 0.1 mM β-ME, and 4 ng/mL FGF-2.  HDF cells were cultured with DMEM including 10% FBS, and 1% penicillin/streptomycin at 37 oC in an atmosphere of 5% CO2. ES cells were culture with medium included DMEM /F12, 20% serum replacement, 1 mM glutamine, 0.1% NEAA, 0.1% penicillin/streptomycin, 0.1 mM β-ME and 4 ng/mL FGF-2.

extract_protocol:
1ug of gDNA was sheared to 200~400bp using the Covaris LE220 sonicator (Covaris Inc. Woburn, MA). The fragmented products were immunoprecipitated using the MethylMiner Methylated DNA Enrichment Kit (Invitrogen) following the manufacture’s recommended protocol. Briefly, Methylated DNA was isolated from fragmented whole genomic DNA by binding to the methyl-CpG binding domain of human MBD2 protein, which is coupled to paramagnetic Dynabeads® M-280 Streptavidin via a biotin linker. The methylated fragments were eluted as a single enriched population with a 2000 mM NaCl elution buffer.
DNA libraries were prepared for sequencing using MethylMiner Methylated DNA Enrichment Kit (Invitrogen).

treatment_protocol: The HDF cells were directly exposed to ultrasound stimulus (20 KHz, 1.0 W/cm2) for 5 sec. And then, 2 x 10^5 cells of ultrasound treated cells were cultured with ultrasound-treated media (20 KHz, 5.0 W/cm2, for 10min) on the 35mm petri dish.

value: skin

value: dermal fibroblast cells


title: 15D_MBD


title: ATAC_aKG_rep2_J13-2


title: ES_MBD

value: embryo

value: embryonic stem cells


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A8_AD002


title: N-346

description: bulk RNA

value: neural

value: brain


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A3_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A3_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A3_AD006


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A7_AD010


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A3_AD007


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A7_AD007


title: 1D_MBD


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A7_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A3_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A3_AD012


title: NGN2

city: New Haven

first: Albert

middle: E.

last: Ayoub

state: CT

address: 333 Cedar St. SHM C317

department: Neurobiology

institute: Yale University

data_processing:
Tophat 1.42
Samtools 0.1.18; samtools sort -n; samtools view
Samtools 0.1.18
RSEQTools 0.6; sam2mrf; mrfQuantifier
Genome_build: hg19/RheMac2/mm9
Supplementary_files_format_and_content: EnsemblGene/RPKM

source_name: Human Radial Glia

extract_protocol:
Qiagen RNAeasy microkit
standard illumina strand specific protocol

tag: tissue/cell type

value: culture

tag: tissue subtype

value: Neurog2 transfection

tag: sample type

value: Magnetic sorting

tag: time point

value: Neurog2 transfection, 3DIV


title: Genomic DNA from glioma sample; IDHmut_GIII_24

type: genomic

city: Clermont-Ferrand

first: franck

last: court

address: 28 Place Henri Dunant

country: France

institute: GReD CNRS

hyb_protocol: Hybridisation on the arrays was performed by Integragen, SA following the Illumina Infinium HD methylation protocol.

scan_protocol: Standard protocol defined by Illumina for HM450K

data_processing: GenomeStudio V2011.1

label: Cy5 and Cy3

source_name: DNA from Glioma sample

label_protocol: Bisulfite conversion was performed by Integragen, SA.

growth_protocol: Immediately after surgery, glioma samples were snap-frozen and stored in liquid nitrogen. non-tumoral control brain samples were cadaveric specimens removed by autopsy 4-16 h following accidental death (Brain and Tissue Bank of Maryland).

extract_protocol: DNA was isolated from frozen tissues using the QIAamp DNA Mini Kit (Qiagen), according to the manufacturer’s recommendations

treatment_protocol: Controls and glioma samples were homogenized by cryogenic grinding.

tag: gliomas classification

value: grade:Grade IIII

value: IDH statut:IDHmut

tag: gender

tag: disease

value: glioma sample


title: Genomic DNA from glioma sample; IDHmut_GIII_32


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A2_AD012

tag: tissue

tag: age


title: NGN1


title: Genomic DNA from glioma sample; IDHmut_GIII_22

value: Female


title: Genomic DNA from glioma sample; IDHmut_GIII_1


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A6_AD010

value: frontal cortex

tag: Sex

tag: donor


title: HRG14W-PM3

value: proliferation media

value: proliferation media, 1DIV


title: HRG14W-PM2


title: Genomic DNA from glioma sample; IDHmut_GII_10

value: grade:Grade II


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A2_AD006

value: Male


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A2_AD008


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A2_AD007


title: HRG14W-PM1


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A6_AD008


title: Genomic DNA from glioma sample; IDHmut_GII_12


title: Genomic DNA from glioma sample; IDHmut_GII_6


title: Genomic DNA from glioma sample; IDHmut_GII_13


title: Genomic DNA from glioma sample; IDHmut_GII_14


title: Genomic DNA from glioma sample; IDHmut_GII_7


title: Genomic DNA from glioma sample; IDHmut_GII_9


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A6_AD007


title: Genomic DNA from glioma sample; IDHmut_GII_4


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A2_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A6_AD006


title: HRG14W-DM3

value: differentiation media

value: differentiation media, 10DIV


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A7_AD004


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A7_AD008


title: HRG14W-DM2


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A6_AD001


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A2_AD002


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A5_AD010


title: HRG14W-DM1


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A1_AD012


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A5_AD008


title: Genomic DNA from glioma sample; IDHmut_GII_5


title: EG2

value: EGFP transfection

value: EGFP transfection, 3DIV


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A1_AD008


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A5_AD007


title: EG1

value: culture


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A1_AD010


title: DNM2

value: dnMAML transfection

value: dnMAML transfection, 3DIV


title: Genomic DNA from glioma sample; IDHmut_GII_3


title: Genomic DNA from glioma sample; IDHwt_GIII_37

value: IDH statut:IDHwt


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A5_AD006


title: Genomic DNA from glioma sample; IDHmut_GII_2


title: Genomic DNA from glioma sample; IDHwt_GIII_38

value: grade:Grade IIII


title: DNM1

value: Magnetic sorting


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A5_AD004


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A1_AD006


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A1_AD004


title: Genomic DNA from glioma sample; IDHwt_GIII_36

value: glioma sample


title: 190305_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A4_AD012


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A1_AD002


title: 181218_snm3Cseq_hs_21yr_BA10_UMB5577_1_UMB5577_2_A12_AD012