title: RNA-seq.duxWT_Sorted_GFPneg_rep2

city: Singapore

first: Zhenhua

last: hu

state: singapore

address: 31 Biopolis drive

country: Singapore

institute: IMCB, A*STAR

data_processing:
Reads were trimmed to remove the adapter sequences and low quality bases using Trim_Galore (v0.4.2_dev).
For RNA-seq, reads mapping to the mouse GRCm38 reference genome, guided by the vM9 gene model from the GENCODE project, and gene expression quantification were carried out by RSEM pipeline. Repetitive elements expression quantification was carried out by RepEnrich pipeline, where repeat annotation in mm10 was obtained from the UCSC genome browser track repeatMasker. Differential gene and Repetitive elements expression was called using DESeq2.
For ATAC-seq, paired-end raw sequencing reads were trimmed with Trim Galore to remove adapters and trim low quality reads. Cleaned reads were then mapped to the mouse reference genome mm10 with Bowtie2 (v.2.2.9) with the following parameters: --N 1 --L 25 --X 2000 --no-mixed --no-discordant. Mapped reads were sorted and PCR duplicates were removed using SAMtools(v1.4). Biological replicate level ATAC-seq differential peaks were called between conditions with MACS2 ‘callpeak’ (--B --nomodel --shift -100 --extsize 200), generating replicate-level bedgraph files, followed by the ‘bdgdiff’ subcommand (--l 500 --g 250) to call ‘differential peaks’. Subsequently, high quality overlapping peaks between two replicates were selected using Bedtools ‘intersect’ (-u -f 0.5 –F 0.5) for condition specific peaks.  For coverage bigwig file generation, replicate-merged bedgraph files were generated using MACS2 ‘callpeak’ (--B –SPMR –nomodel --shift -100 --extsize 200 –t replicate1.bam replicate2.bam), which were subsequently converted to bigwig files using “bedGraphToBigWig” from Kent informatics. To detect the chromatin accessibility across repetitive element regions, mapped reads were further filtered with SAMtools (view –q 10) to remove low-quality multiple-mapping reads, and coverage bigwig files were generated as mentioned.
For ChIP-seq, paired-end raw sequencing reads were trimmed with Trim Galore to remove adapters and trim low quality reads. Cleaned reads were then mapped to the mouse reference genome mm10 with Bowtie2 (v.2.2.9) with the following parameters: --N 1 --L 25 --X 2000 --no-mixed --no-discordant. Mapped reads were sorted and PCR duplicates were removed using SAMtools(v1.4). Biological replicate BAM files were merged for IP and input respectively before peak calling.  MACS2 ‘callpeak’ (-t  NELFA_mergedIP.rmdup.bam  -c NELFA_mergedInput.rmdup.bam -n NELFA_mm10 -g mm -f BAMPE --keep-dup all), was used to call NELFA peaks. Peaks were filtered out if p ≥ 10e-09 or overlapping a blacklist region in mm10.
Genome_build: mm10
Supplementary_files_format_and_content: BIGWIG files contain theATAC-seq and ChIP-seq coverage; EXCEL file contains counts and deseq2 results for differential gene and TE expression analyses, condition specific ATAC gained and lost regions, and NELFA enriched regions from ChIP-seq.

source_name: mESCs

growth_protocol: mESCs were cultured on gelatin coated cell culture dishes with knockout DMEM supplemented with serum, LIF and 2i.

extract_protocol:
RNA-seq: Total RNA was extracted using TRI-reagent and purified using the Direct-zol RNA MiniPrep Kit by Zymo. ATAC-Seq: 2-DG treated and Dox-induced mESCs were FACs sorted to enrich for both GFP- positive and -negative populations. Collected mESCs were lyzed in hypotonic buffer containing NP-40. Isolated cell nuclei were then subjected tagmentation with Illumina Tn5 transposase before PCR amplification with NEBNext 2X PCR mastermix and Illumina Nextera indexed primers. ChIP-seq: Dox-induced were fixed with formaldehyde (final 1%) for 10 minutes. Fixed cells were then subjected to standard ChIP lysis protocol to release chromatin. Released Chromatin was sonicated for 15 cycles (30 sec on, 30 sec off) on a Biorupter at high setting. Soluble chromatin was collected and between 1-2 mg was used for IP with 4-8 ug of respective Dyna-protein G conjugated antibody complex. Captured fragments were subjected to several rounds of stringent salt washes before eluting with SDS buffer. ChIP-seq: Dox-induced were fixed with formaldehyde (final 1%) for 10 minutes. Fixed cells were then subjected to standard ChIP lysis protocol to release chromatin. Released Chromatin was sonicated for 15 cycles (30 sec on, 30 sec off) on a Biorupter at high setting. Soluble chromatin was collected and between 1-2 mg was used for IP with 4-8 ug of respective Dyna-protein G conjugated antibody complex. Captured fragments were subjected to several rounds of stringent salt washes before eluting with SDS buffer.
RNA-seq: 1µg of total RNA was used as input for library preparation; first, ribosomal depletion was carried using the NEBNext rRNA depletion kit (Cat: E6310S) and RNA-seq libraries were constructed using the NEBNext RNA Ultra II directional library kit (Cat: E7760S) as per manufacturer’s instructions. Average peak distribution of purified libraries was assessed on an Agilent Technologies 2100 Bioanalyzer using a DNA 1000 kit (Cat: 5067-1504). The concentration of adapter-ligated libraries was subsequently assessed using the Kapa Biosystems Kapa Library Quant Kit (cat#KK4824). Individual libraries were then pooled in equimolar amounts to a final concentration 5nM in preparation for sequencing. Average peak distribution of purified libraries was assessed on an Agilent Technologies 2100 Bioanalyzer using a DNA 1000 kit (Cat: 5067-1504). The concentration of adapter-ligated libraries was subsequently assessed using the Kapa Biosystems Kapa Library Quant Kit (cat#KK4824). Individual libraries were then pooled in equimolar amounts to a final concentration 5nM in preparation for sequencing. ChIP-seq: Eluted DNA was purified and subjected to NGS library preparation using the NEBNext DNA ultra II kit. Constructed ChIP libraries were sequenced on the Illumina NextSeq or HiSeq platform.

treatment_protocol: In RNA-seq, Culture media was supplemented with 4mM 2-DG for 4 days to induce NELFA for reporter mESC. 0.4 µg/ml of Dox was used to induce NELFA for Dox-inducible mESC for 16 hours.  In ATAC-seq, Culture media was supplemented with 4mM 2-DG for 4 days; to induce NELFA for reporter mESC, and 0.4ug/mL of Doxycycline for Dox-inducible NELFA mESCs. In ChIP-seq, E14 cells were cultured in ‘2i’ DMEM knockout medium containing 0.4ug/mL of Doxycycline for 16 hours to induce expression of transgene. In ChIP-seq, E14 cells were cultured in ‘2i’ DMEM knockout medium containing 0.4ug/mL of Doxycycline for 16 hours to induce expression of transgene

tag: experiment type

value: RNA-seq

tag: condition

value: DUXWT, GFP negative, FACS sorted


title: RNA-seq.duxWT_Sorted_GFPpos_rep1

status: Public on Nov 18 2019

type: SRA

library_source: transcriptomic

molecule: total RNA

organism: Mus musculus

value: DUXWT, GFP positive, FACS sorted


title: mf_4_ES

city: Edinburgh

first: Amy

last: Buck

address: Ashworth Labs, Charlotte Auerbach Road

department: IIIR

country: United Kingdom

institute: The University of Edinburgh

data_processing:
Primers were trimmed from the raw fastq format sequence data using cutadapt software (v1.9.1; parameters -O 6 -m 16 -n 5 -q 20).
Only trimmed reads that contained the adapter, were 16nt or greater in length and were present at 2 or more copies per sample were retained.
Simple scripts were used to generate non-redundant sequence sets (collapsed) in fasta format from each sample.
Genome_build: https://parasite.wormbase.org/Litomosoides_sigmodontis_prjeb3075 version nLs.2.1
Supplementary_files_format_and_content: Processed data files contain the non-redundant trimmed sequences from each sample (sequence and counts).

organism: Litomosoides sigmodontis

source_name: microfilariae ES products

growth_protocol: Parasites were obtained at different time points post-infection from Meriones unguiculatus, and cultured in vitro in RPMI-1640 containing 1 mg/ml Penicilin/Streptomycin, 1 mg/ml gentamycin, and 1% D-glucose, at 37C and 5% CO2 for up to 72h.

extract_protocol:
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody.
Small RNA libraries were prepared using TriLink small RNA library preparation kit (Illumina compatible), following manufacturer's recommendations.

tag: developmental stage

value: microfilariae


title: RNA-seq.duxWT_Sorted_GFPneg_rep1


title: Pat5_WM_2_4

city: Freiburg

first: Sagar

address: Hugstetter Straße 55

department: Department of Internal Medicine II

country: Germany

institute: University Medical Center Freiburg

description: library of 192 cells

data_processing:
For image aquisition, intensity extraction and basecalling HiSeq Control Software  2.0.2, RTA 2.4.11 / Recipe Fragment 2.0.0.2 was used. Conversion of bcl2fastq files was performed using bcl2fastq 2.17.1.14
Paired end reads were aligned to the transcriptome using bwa (version 0.6.2-r126) with default parameters. The transcriptome contained all gene models based on the mouse ENCODE VM9 release downloaded from the UCSC genome browser comprising 57,207 isoforms derived from 57,207 gene loci with 57,114 isoforms mapping to fully annotated chromosomes (1 to 19, X, Y, M). All isoforms of the same gene were merged to a single gene locus. Furthermore, gene loci overlapping by >75% were merged to larger gene groups. This procedure resulted in 34,111 gene groups.
The right mate of each read pair was mapped to the ensemble of all gene loci and to the set of 92 ERCC spike-ins in sense direction. Reads mapping to multiple loci were discarded. The left read contains the barcode information: the first six bases correspond to the cell specific barcode followed by six bases representing the unique molecular identifier (UMI). The remainder of the left read contains a poly(T) stretch. The left read was not used for quantification. For each cell barcode, the number of UMIs per transcript was counted and aggregated across all transcripts derived from the same gene locus.
Based on binomial statistics, the number of observed UMIs was converted into transcript counts (Gruen et al., 2014).
Genome_build: ENCODE VM9
Supplementary_files_format_and_content: CSV files, columns represent each cell barcode (total barcodes used = 192), rows represent the geneid and the values in the file are the quantified number of transcripts.

organism: Homo sapiens

source_name: Human brain surgery sample

extract_protocol:
Fresh tissue was dissected by removing the meninges. Single-cell suspesions were generated with a dounce potter followed by passing through a cell strainer. Myelin was removed using percoll gradient centrifugation.
As described in mCEL-Seq2 protocol (Hashimshony et al. 2016 and Herman et al. 2018)
Adapted from TruSeq Small RNA Library Preparation Protocol

tag: tissue

value: Brain

tag: diagnosis

value: Control

tag: region

value: Temporal lobe


title: LC, WT rep2

city: Rehovot

first: Ayelet

last: Erez

address: 234 Herzl Street

department: Biological Regulation

country: Israel

institute: Weizmann Institute of Science

data_processing:
Fastq files for each sample were generated by the usage of bcl2fastq-v2.17.1.14 software (basecalling)
Reads were trimmed using cutadapt
Reads were aligned to the mouse genome mm10 using, TopHat (v2.0.10).
Counting reads on reference genome, RefSeq release mm10, was done with HTSeq-count (version 0.6.1p1)
Differential analysis was carried out using DESeq2 (1.6.3)
Genome_build: mm10
Supplementary_files_format_and_content: processed1_raw_counts.xlsx
Supplementary_files_format_and_content: processed2_normalized_counts.xlsx

source_name: Locus coeruleus

extract_protocol:
Brains were removed rapidly, embedded and frozen in OCT on dry ice without fixation. 8μm thick sections were cut from the frozen block, mounted on polyethylene membrane-coated glass slides and kept in -80°C. At the day of the experiment, slides were thawed for 5 minutes in room temperature followed by fixation in 70% ethanol (30s), incubation in DEPC water, stained with 150µl 1% crystal violet acetate, and washed vigorously in 100% ethanol for a total of 30s and air dried for 3m before microdissection. Tissue sections were microdissected using a UV laser-based PALM Microbeam.
SMARTer Ultra Low Input RNA followed by in-house DNA Seq protocol

tag: strain

tag: age

value: post natal day 35

tag: genotype

value: WT


title: mf_3_ES


title: RNA-seq.duxKO_Sorted_GFPpos_rep2

value: DUX KO, GFP positive, FACS sorted


title: RNA-seq.duxKO_Sorted_GFPpos_rep1


title: Pat5_WM_1_3

description: library of 192 cells


title: LC, WT rep1


title: mf_2_ES


title: Pat5_GM_2_2

description: library of 192 cells


title: RNA-seq.duxWT_Sorted_GFPpos_rep2


title: RNA-seq colon RPS (rep 2)

city: Gainesville

first: Liang

last: Zhou

state: FL

department: Department of Infectious Diseases and Immunology

country: USA

institute: University of Florida

description:
RPS_2
processed data file: Colon_counts.txt

data_processing:
[RNA-seq] De-multiplexed raw data files from the sequencing core were analyzed for quality control using FastQC (Babraham Bioinformatics). Reads were mapped (STAR aligner) to the mm10 assembly of the Mus musculus genome and filtered for uniquely mapped reads. Quantitated relative mRNA expression levels (normalized read counts) were calculated based on exon regions using STAR and the mm10 reference genome annotations.
[16S rRNA-seq] Sequence analyses were performed using QIIME v.1.9.0. After checking the quality of the sequenced reads, 8 nucleotides (nt) barcodes were extracted from both forward and reverse reads to generate a barcode library. Forward and reverse reads were then joined, and sequence libraries were split based on their corresponding barcodes. We used an open reference operational taxonomic unit (OTU) picking strategy to select OTUs (with 97% identity threshold). Taxonomy was assigned based on the Greengenes reference database. A taxonomic table for each taxonomic level was generated based on the OTU table.
Genome_build: mm10 (GRCm38)
Supplementary_files_format_and_content: [RNA-seq] Colon_counts.txt: Tab-delimited text file includes count values for each sample.
Supplementary_files_format_and_content: [16S rRNA-seq] *_table.from_biom_w_taxonomy.txt: Tab-delimited text files include count values for each sample.

source_name: Colon

extract_protocol:
Whole proximal colon tissue (30mg) from Rag1–/– mice gavaged with Rag1–/– feces or RPS 2° feces (two biological replicates for each group) was homogenized in Trizol reagent (Invitrogen).
RNA was subsequently extracted and total RNA was treated with poly-A enrichment kit and RNA-seq libraries were generated.

tag: mouse strain

tag: mouse genotype/variation

value: Proximal colon

tag: gavage

value: Rag1-/- Selplg-/- feces


title: RNA-seq colon RPS (rep 1)

description:
RPS_1
processed data file: Colon_counts.txt


title: mf_1_ES


title: Pat5_GM_1_1

description: library of 192 cells


title: gAF_12_ES

source_name: adult female worms ES products pooled

value: adult

tag: Sex

value: female


title: RNA-seq colon Rag1‒/‒ (rep 2)

description:
RagKO_2
processed data file: Colon_counts.txt

value: Rag1-/- feces


title: Pat4_WM_1_12

description: library of 192 cells

value: Frontal lobe


title: RNA-seq.duxKO_Sorted_GFPneg_rep1

value: DUX KO, GFP negative, FACS sorted


title: Pat6_Plate1_1_1

description: library of 192 cells


title: LC, WT rep3


title: gAF_11_ES


title: Pat4_WM_1_11

description: library of 192 cells


title: RNA-seq colon Rag1‒/‒ (rep 1)

description:
RagKO_1
processed data file: Colon_counts.txt


title: NT1

type: RNA

city: Seoul

first: DONG-MYUNG

last: SHIN

address: Pungnap-2 dong, Songpa-gu

department: Department of Biomedical Sciences

country: South Korea

institute: University of Ulsan College of Medicine

description: control replicate#1

hyb_protocol: Approximately 5.5 μg of labeled DNA target was hybridized to the Affymetrix GeneChip Human 2.0 ST Array at 45°C for 16hour.

scan_protocol: Hybridized arrays were washed and stained on a GeneChip Fluidics Station 450 and scanned on a GCS3000 Scanner (Affymetrix).

data_processing: Raw data were extracted automatically in Affymetrix data extraction protocol using the software provided by Affymetrix GeneChip® Command Console® Software (AGCC). After importing CEL files, the data were summarized and normalized with *robust multi-average (RMA) method* implemented in Affymetrix   Expression Console Software (EC).

label: biotin-labeled with TdT

source_name: murine embryo

label_protocol: Biotin-labeled with TdT (terminal deoxynucleotidyl transferase) using the GeneChip WT Terminal labeling kit

growth_protocol: R1 mESCs were grown in 15% heat-inactivated fetal bovine serum (FBS; HyClone, Pittsburgh, PA) and 100 IU/ml ESGRO/LIF (Millipore, Billerica, MA) on a 0.1% gelatin coated tissue culture at 37'C 5% CO2 conditions

extract_protocol: Total RNA from various cells was isolated using the RNeasy Mini Kit (QIAGEN, Valencia, CA), including treatment with DNase I (QIAGEN)

treatment_protocol: R1 mESCs were maintained in the abasence (NT) or presence (250ug) of ascorbic acid 2-glucose (AA2G) of 0.74mM (250 ug/ml) for 3 days

tag: gender

value: male

value: inner cell mass

tag: cell type

value: embryonic stem cell, R1

value: embryo


title: gAF_10_ES


title: Liver, LD Tun 2

city: Shanghai

first: Yan

last: Lu

address: 180 Fenglin Road

department: Department of Endocrinology and Metabolism

country: China

institute: Zhongshan Hospital, Fudan University

data_processing:
Basecalls performed using CASAVA version 1.8
After 3’ adaptor-trimming and low quality reads removing by cutadapt software (v1.9.3), the high quality clean reads were aligned to the reference genome (UCSC MM10) with hisat2 software (v2.0.4).
Then, guided by the Ensembl gtf gene annotation file, cuffdiff software (part of cufflinks) was used to get the gene level FPKM as the expression profiles of mRNA, and fold change and p-value were calculated based on FPKM, differentially expressed mRNA were identified.
Genome_build: mm10
Supplementary_files_format_and_content: Counts, FPKMs

source_name: Liver tissues

growth_protocol: Eight weeks old male C57BL/6 mice were purchased from the Shanghai Laboratory Animal Company (SLAC). Sustained ER stress was induced with daily intraperitoneal injections of 0.2 mg/kg tunicamycin (654380, Sigma-Aldrich) for 14 days.Then mice were sacrificed and total RNAs were isoloated from liver tissues.

extract_protocol:
Total RNA (1 μg) was used for removing the rRNAs using Ribo-Zero rRNA Removal Kits (Illumina, San Diego, CA, USA) following the manufacturer's instructions.
RNA libraries were constructed by using rRNA-depleted RNAs with TruSeq Stranded Total RNA Library Prep Kit (Illumina, San Diego, CA,  USA) according to the manufacturer’s instructions. Libraries were controlled for quality and quantified using  the BioAnalyzer 2100 system (Agilent Technologies, Inc., USA).

treatment_protocol: Mice were housed at 21±1°C with a humidity of 55±10% and a 12-hour light–dark cycle.

value: Liver

tag: agent

value: tunicamycin

value: 10 weeks


title: Pat4_GM_2_6

description: library of 192 cells

value: Brain

value: Control


title: gAF_8_ES


title: Liver, Control 3

value: control


title: Pat4_GM_1_5

description: library of 192 cells


title: gAF_9_ES


title: Pat3_WM_2_4

description: library of 192 cells

value: Temporal lobe


title: Liver, Control 2


title: gAF_7_ES


title: Pat3_GM_2_2

description: library of 192 cells


title: Pat3_WM_1_3

description: library of 192 cells


title: P60WTtx4

city: PARIS

first: Pauline

last: ARNAUD

address: 46, rue Henri Huchard

country: France

institute: AP-HP - Hôpital Bichat

description:
WT mice - age P60 - treated with baclofen since P16
Mice_cuffdiff_results, Mice_cuffdiff_DEGs

data_processing:
Random read removal from all samples of the same age to correct for read imbalances (after the read removal all samples of the same age have the read count of that sample of that age with the lowest read count)
custom C# script
Alignement to Reference genome
Tophat 2.0.8, samtools-0.1.7, bowtie 2.1.0
Identification of differentially expressed genes
Cufflinks 1.3.0
Genome_build: mm9
Supplementary_files_format_and_content: mouse and human ensemble refernce annotation
Supplementary_files_format_and_content: Mice_cuffdiff_results: cuffdiff output including average gene expression values in whole aortic wall from P16 MFS vs WT mice, baclofen treated P60 WT vs untreated WT mice and baclofen treated P60 MFS vs untreated MFS mice
Supplementary_files_format_and_content: Mice_cuffdiff_DEGs: List of differentially expressed genes in whole aortic wall from P16 MFS vs WT mice, baclofen treated P60 WT vs untreated WT mice and baclofen treated P60 MFS vs untreated MFS mice

source_name: Thoracic aortic tissue

extract_protocol: none provided by the submitter

value: WT

tag: treatment

value: baclofen


title: Liver, Control 1


title: ChIP-seq.NELFA_plusDox_rep3_input

description:
GSE113671_mNELFA_DOXplus_ChIP_input.bw
GSE113671_NELFA_ChIP_peaks.narrowPeaks.gz

library_source: genomic

data_processing:
Reads were trimmed to remove the adapter sequences and low quality bases using Trim_Galore (v0.4.2_dev).
For ChIP-seq, paired-end raw sequencing reads were trimmed with Trim Galore to remove adapters and trim low quality reads. Cleaned reads were then mapped to the mouse reference genome mm10 with Bowtie2 (v.2.2.9) with the following parameters: --N 1 --L 25 --X 2000 --no-mixed --no-discordant. Mapped reads were sorted and PCR duplicates were removed using SAMtools(v1.4). Biological replicate BAM files were merged for IP and input respectively before peak calling.  MACS2 ‘callpeak’ (-t  NELFA_mergedIP.rmdup.bam  -c NELFA_mergedInput.rmdup.bam -n NELFA_mm10 -g mm -f BAMPE --keep-dup all), was used to call NELFA peaks. Peaks were filtered out if p ≥ 10e-09 or overlapping a blacklist region in mm10.
Genome_build: mm10
Supplementary_files_format_and_content: BIGWIG files contain ChIP-seq coverage and narrowPeak file includes called peaks.

molecule: genomic DNA

extract_protocol:
ChIP-seq: Dox-induced were fixed with formaldehyde (final 1%) for 10 minutes. Fixed cells were then subjected to standard ChIP lysis protocol to release chromatin. Released Chromatin was sonicated for 15 cycles (30 sec on, 30 sec off) on a Biorupter at high setting. Soluble chromatin was collected and between 1-2 mg was used for IP with 4-8 ug of respective Dyna-protein G conjugated antibody complex. Captured fragments were subjected to several rounds of stringent salt washes before eluting with SDS buffer.
ChIP-seq: Eluted DNA was purified and subjected to NGS library preparation using the NEBNext DNA ultra II kit. Constructed ChIP libraries were sequenced on the Illumina NextSeq platform.

treatment_protocol: In ChIP-seq, E14 cells were cultured in ‘2i’ DMEM knockout medium containing 0.4ug/mL of Doxycycline for 16 hours to induce expression of transgene. In ChIP-seq, E14 cells were cultured in ‘2i’ DMEM knockout medium containing 0.4ug/mL of Doxycycline for 16 hours to induce expression of transgene

value: Mouse embryonic stem cells (mESCs)

value: Dox treated

tag: chip antibody

value: none (input)


title: gAF_5_ES

value: adult

value: female


title: gAF_6_ES


title: gAF_4_ES


title: P60WTtx3


title: ChIP-seq.NELFA_plusDox_rep3_HAantibody2

description:
GSE113671_mNELFA_DOXplus_ChIP_IP.bw
GSE113671_NELFA_ChIP_peaks.narrowPeaks.gz

tag: condition


title: Pat2_WM_2_4

description: library of 192 cells


title: Pat2_WM_1_3

description: library of 192 cells


title: gAF_3_ES

tag: developmental stage


title: gAF_2_ES


title: P60WTtx2


title: P60WTtx1


title: Pat2_GM_2_2

description: library of 192 cells

tag: tissue


title: ChIP-seq.NELFA_plusDox_rep3_HAantibody1


title: P60MTtx4

description:
Fbn1 mgR/mgR mice - age P60 - treated with baclofen since P16
Mice_cuffdiff_results, Mice_cuffdiff_DEGs

value: Fbn1 mgR/mgR


title: ChIP-seq.NELFA_plusDox_rep2_HAantibody1


title: AM_10_ES

source_name: adult male worms ES products pooled

value: male


title: P60MTtx3


title: Pat3_GM_1_1

description: library of 192 cells

tag: diagnosis


title: AM_9_ES


title: ChIP-seq.NELFA_plusDox_rep1_HAantibody1


title: P60MTtx2


title: P60MTtx1


title: AM_8_ES


title: Pat1_GM_2_6

description: library of 192 cells

tag: region


title: P60WTnt3

description:
WT mice - age P60 - treated with vehicle since P16
Mice_cuffdiff_results, Mice_cuffdiff_DEGs

tag: strain

value: vehicle


title: ATAC-seq.Sorted-plusDox_rep2

data_processing:
Reads were trimmed to remove the adapter sequences and low quality bases using Trim_Galore (v0.4.2_dev).
For ATAC-seq, paired-end raw sequencing reads were trimmed with Trim Galore to remove adapters and trim low quality reads. Cleaned reads were then mapped to the mouse reference genome mm10 with Bowtie2 (v.2.2.9) with the following parameters: --N 1 --L 25 --X 2000 --no-mixed --no-discordant. Mapped reads were sorted and PCR duplicates were removed using SAMtools(v1.4). Biological replicate level ATAC-seq differential peaks were called between conditions with MACS2 'callpeak' (--B --nomodel --shift -100 --extsize 200), generating replicate-level bedgraph files, followed by the 'bdgdiff' subcommand (--l 500 --g 250) to call 'differential peaks'. Subsequently, high quality overlapping peaks between two replicates were selected using Bedtools 'intersect' (-u -f 0.5 'F 0.5) for condition specific peaks.  For coverage bigwig file generation, replicate-merged bedgraph files were generated using MACS2 'callpeak' (--B 'SPMR 'nomodel --shift -100 --extsize 200 't replicate1.bam replicate2.bam), which were subsequently converted to bigwig files using 'bedGraphToBigWig' from Kent informatics. To detect the chromatin accessibility across repetitive element regions, mapped reads were further filtered with SAMtools (view 'q 10) to remove low-quality multiple-mapping reads, and coverage bigwig files were generated as mentioned.
Genome_build: mm10
Supplementary_files_format_and_content: BIGWIG files contain theATAC-seq coverage; EXCEL file contains condition specific ATAC gained and lost regions.

extract_protocol: ATAC-Seq: 2-DG treated and Dox-induced mESCs were FACs sorted to enrich for both GFP- positive and -negative populations. Collected mESCs were lyzed in hypotonic buffer containing NP-40. Isolated cell nuclei were then subjected tagmentation with Illumina Tn5 transposase before PCR amplification with NEBNext 2X PCR mastermix and Illumina Nextera indexed primers.

treatment_protocol: In ATAC-seq, Culture media was supplemented with 4mM 2-DG for 4 days to induce NELFA for reporter mESC, and 0.4ug/mL of Doxycycline for Dox-inducible NELFA mESCs.

value: Dox treated, GFP positive, FACS-sorted


title: ChIP-seq.NELFA_plusDox_rep2_input


title: AM_7_ES


title: Pat1_GM_1_5

description: library of 192 cells


title: gAF_1_ES


title: AM_6_ES


title: Pat15_WM_2_4

description: library of 192 cells


title: ChIP-seq.NELFA_plusDox_rep1_input


title: ATAC-seq.Sorted-plus2DG_rep2

value: 2DG treated, GFP positive, FACS-sorted


title: P60MTnt3

description:
Fbn1 mgR/mgR mice - age P60 - treated with vehicle since P16
Mice_cuffdiff_results, Mice_cuffdiff_DEGs


title: Pat1_WM_2_8

description: library of 192 cells


title: Pat1_WM_1_7

description: library of 192 cells


title: AM_5_ES


title: ATAC-seq.Sorted-noDox_rep2

value: None Dox treated, GFP negative, FACS-sorted


title: P60MTnt2


title: AM_4_ES


title: RNA-seq.duxKO_Sorted_GFPneg_rep2

tag: experiment type

value: RNA-seq

value: DUX KO, GFP negative, FACS sorted


title: P60MTnt1

tag: genotype


title: ATAC-seq.Sorted-noDox_rep1


title: Pat2_GM_1_1

description: library of 192 cells

value: Brain

value: Control


title: Pat15_GM_1_1

description: library of 192 cells

value: Temporal lobe


title: AM_3_ES


title: ATAC-seq.Sorted-plusDox_rep1


title: P16WT3

description:
WT mice - age P16
Mice_cuffdiff_results, Mice_cuffdiff_DEGs

value: WT

value: no treatment


title: P60WTnt2


title: ATAC-seq.Sorted-no2DG_rep2

value: Mouse embryonic stem cells (mESCs)

value: None 2DG treated, GFP negative, FACS-sorted


title: Pat14_Plate2_2_4

description: library of 192 cells


title: P16WT2


title: AM_2_ES

value: adult


title: AM_1_ES


title: ATAC-seq.Sorted-no2DG_rep1


title: P16WT1


title: Pat14_Plate2_1_3

description: library of 192 cells


title: pgAF_2_ES

source_name: pregravid adult female worms ES products pooled

value: young adult

value: female


title: Pat14_Plate1_2_2

description: library of 192 cells


title: Pat15_GM_2_2

description: library of 192 cells


title: P16MT3

description:
Fbn1 mgR/mgR mice - age P16
Mice_cuffdiff_results, Mice_cuffdiff_DEGs

value: Fbn1 mgR/mgR


title: RNA-seq.Sorted-noDox_rep2

data_processing:
Reads were trimmed to remove the adapter sequences and low quality bases using Trim_Galore (v0.4.2_dev).
For RNA-seq, reads mapping to the mouse GRCm38 reference genome, guided by the vM9 gene model from the GENCODE project, and gene expression quantification were carried out by RSEM pipeline. Repetitive elements expression quantification was carried out by RepEnrich pipeline, where repeat annotation in mm10 was obtained from the UCSC genome browser track repeatMasker. Differential gene and Repetitive elements expression was called using DESeq2.
Genome_build: mm10
Supplementary_files_format_and_content:  EXCEL file contains counts and deseq2 results for differential gene and TE expression analyses.

extract_protocol:
RNA-seq: Total RNA was extracted using TRI-reagent and purified using the Direct-zol RNA MiniPrep Kit by Zymo.
RNA-seq: 1µg of total RNA was used as input for library preparation; first, ribosomal depletion was carried using the NEBNext rRNA depletion kit (Cat: E6310S) and RNA-seq libraries were constructed using the NEBNext RNA Ultra II directional library kit (Cat: E7760S) as per manufacturer’s instructions. Average peak distribution of purified libraries was assessed on an Agilent Technologies 2100 Bioanalyzer using a DNA 1000 kit (Cat: 5067-1504). The concentration of adapter-ligated libraries was subsequently assessed using the Kapa Biosystems Kapa Library Quant Kit (cat#KK4824). Individual libraries were then pooled in equimolar amounts to a final concentration 5nM in preparation for sequencing.

treatment_protocol: In RNA-seq, Culture media was supplemented with 4mM 2-DG for 4 days to induce NELFA for reporter mESC. 0.4 µg/ml of Dox was used to induce NELFA for Dox-inducible mESC for 16 hours.

value: no dox


title: RNA-seq.Sorted-plusDox_rep2

value: 16hr dox-induction, FACS-sorted


title: pgAF_1_ES


title: P16MT2


title: Pat14_Plate1_1_1

description: library of 192 cells


title: jAM_2_ES

source_name: juvenile male worms ES products pooled

value: juvenile

value: male


title: Pat13_GM_2_2

description: library of 192 cells


title: jAM_1_ES


title: Pat13_GM_1_1

description: library of 192 cells


title: RNA-seq.Sorted-noDox_rep1

extract_protocol:
RNA-seq: Total RNA was extracted using TRI-reagent and purified using the Direct-zol RNA MiniPrep Kit by Zymo.
RNA-seq: 1µg of total RNA was used as input for library preparation; first, ribosomal depletion was carried using the NEBNext rRNA depletion kit (Cat: E6310S) and RNA-seq libraries were constructed using the NEBNext RNA Ultra II directional library kit (Cat: E7760S) as per manufacturer’s instructions. Average peak distribution of purified libraries was assessed on an Agilent Technologies 2100 Bioanalyzer using a DNA 1000 kit (Cat: 5067-1504). The concentration of adapter-ligated libraries was subsequently assessed using the Kapa Biosystems Kapa Library Quant Kit (cat#KK4824). Individual libraries were then pooled in equimolar amounts to a final concentration 5nM in preparation for sequencing.  NOMe-seq: 0.5 µg of purified genomic DNA was sonicated on a Covaris S220 using the 150bp settings. Fragmented genomic DNA was purified with SPRI beads and sequencing libraries were prepared using the NEBNext DNA Ultra II kit (NEB, Cat: E7645S) with methylated adapters (NEB, Cat: E7535S) as per manufacturer’s instructions. Average peak distribution of purified libraries was assessed on an Agilent Technologies 2100 Bioanalyzer using a DNA 1000 kit (Cat: 5067-1504). The concentration of adapter-ligated libraries was subsequently assessed using the Kapa Biosystems Kapa Library Quant Kit (cat#KK4824). Individual libraries were then pooled in equimolar amounts to a final concentration 5nM in preparation for sequencing


title: L4_2_ES

source_name: L4 larvae ES products

value: L4 larvae


title: RNA-seq.Sorted-plusDox_rep1


title: P16MT1


title: Pat12_2_2

description: library of 192 cells


title: Cells_infected_4

organism: Meriones unguiculatus

source_name: Infected gerbil adherent cells

tag: group

value: Infected gerbil adherent cells


title: L4_1_ES


title: RNA-seq.Unsorted-plus2DG_rep2

value: 2DG treated for 4 days


title: Pat12_1_1

description: library of 192 cells

value: Brain

value: Control


title: Cells_infected_3


title: RNA-seq.Unsorted-no2DG_rep2

value: no 2DG


title: hL32_ES

source_name: host-derived L3 larvae ES products

value: host-derived L3 larvae


title: Pat11_Pl2_1_8

description: library of 192 cells

value: Temporal lobe


title: RNA-seq.Unsorted-plus2DG_rep1

value: Mouse embryonic stem cells (mESCs)


title: Cells_infected_2


title: Pat11_Pl2_1_7

description: library of 192 cells


title: hL31_ES


title: Cells_infected_1


title: ATAC-seq.Sorted-plus2DG_rep1

value: 2DG treated, GFP positive, FACS-sorted


title: vL3s_2_ES

source_name: vector-derived L3 larvae ES products

value: vector-derived L3 larvae


title: RNA-seq.Sorted-GFPneg-rep2

value: GFP negative, FACS sorted


title: Cells_infected_5


title: Cells_naive_3

source_name: Naive gerbil adherent cells

value: Naive gerbil adherent cells


title: Pat11_Pl1_1_5

description: library of 192 cells


title: gAF_ES_polyP_rep_2

value: adult

value: female


title: RNA-seq.Sorted-GFPneg-rep1

source_name: mESCs


title: RNA-seq.Sorted-GFPpos-rep2

value: GFP positive, FACS sorted


title: Cells_naive_2


title: vL3s_1_ES


title: gAF_ES_rep_2


title: RNA-seq.Unsorted-no2DG_rep1


title: Cells_naive_1


title: Pat10_GM_2_6

description: library of 192 cells


title: gAF_ES_polyP_rep_1


title: RNA-seq.Sorted-GFPpos-rep1


title: Pat10_WM_2_8

description: library of 192 cells


title: Pat10_GM_1_5

description: library of 192 cells


title: Serum_infected_4

source_name: Infected gerbil serum

value: Infected gerbil serum


title: gAF_ES_rep_1


title: Pat11_Pl1_2_6

description: library of 192 cells

source_name: Human brain surgery sample


title: GBM4_2_8

description: library of 192 cells

value: Glioblastoma multiforme


title: Liver, LD Tun 1

value: male

value: Liver

value: tunicamycin

tag: age

value: 10 weeks


title: Serum_infected_3


title: Serum_infected_5


title: GBM4_1_7

description: library of 192 cells

value: Brain


title: Male_worms_PolyP_rep_2

source_name: adult male worms


title: Serum_infected_2


title: D135N1

city: Beijing

first: Tianpei

last: Shi

address: No. 2, Yuanmingyuan West Road, Huangzhuang, Haidian

institute: Chinese Academy of Agricultural Sciences

data_processing:
After base calling, Cutadapt was used to remove the reads that contained adaptor contamination, low quality basea and undertermined bases.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to Oar 3.1 whole genome using bowtie2 and Tophat2 with parameters Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using a protocol.
Genome_build: Oar 3.1
Supplementary_files_format_and_content: tab-delimited text file includes RPKM values for each Sample.

organism: Ovis aries

source_name: longissimus dorsi

extract_protocol:
longissimus dorsi were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. mRNA-Seq sample preparation kit (Illumina, San Diego, USA)   TruSeq Small RNA Sample Prep Kits(Illumina, San Diego, USA) were used with 10 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols

tag: breed

value: Chinese merino

value: longissimus dorsi muscle

value: Pregnancy day 135


title: D105N3

value: Pregnancy day 105


title: Male_worms_rep_2

tag: Sex


title: GBM3_Pl2_2_8

description: library of 192 cells

value: Temporal lobe


title: D105N2


title: Serum_infected_1


title: GBM3_Pl2_1_7

description: library of 192 cells


title: Male_worms_rep_1


title: Male_worms_PolyP_rep_1


title: Serum_naive_3

source_name: Naive gerbil serum

value: Naive gerbil serum


title: D105N1


title: GBM3_Pl1_2_6

description: library of 192 cells


title: D85N3

value: Pregnancy day 85


title: Serum_naive_2


title: D135N2


title: Serum_naive_1


title: GBM3_Pl1_1_5

description: library of 192 cells


title: D85N2


title: D85N1


title: gAF_worms_rep_2

source_name: adult female worms

value: adult


title: GBM2_GBM_2_6

description: library of 192 cells

value: Frontal lobe


title: gAF_worms_PolyP_rep_2

value: female


title: gAF_worms_rep_1


title: Cerebellum, Z-XN-1

city: Chengdu

first: Jiabo

last: Wang

state: Sichuan

address: First Roll street, 4th south part, NO.16

institute: Southwest Minzu University

description: Zang Yakow

data_processing:
Basing on Illumina HiSeqTM 4000 platform, we got paired-end sequencing reads of 18 tissue samples. The sequencing reads were filtered by fastp (version 0.19.8) software with default parameters. Clean reads were aligned to the Yak reference genome (GCF_000298355.1 BosGru v2.0, Ensemble) with HISAT2 (version 2.1.0) to create Sam file. After sorting and converting to bam, we used StringTie (version 1.2.3) to assemble transcripts for each sample. All transcripts of samples were merged together by merge function of StringTie following all sample file name and created output files for Ballgown format. The merged file was annotated with reference genome annotation file (GCF_000298355.1 BosGru v2.0, Ensemble) by gffcompare (version 0.10.8).
fastp (version 0.19.8)
HISAT2 (version 2.1.0)
StringTie (version 1.2.3)
Ballgown (R)
gffcompare (version 0.10.8)
Genome_build: GCF_000298355.1
Supplementary_files_format_and_content: FPKMs, TPMs

organism: Bos grunniens x Bos taurus

source_name: Cerebellum

extract_protocol:
All clean tissue samples were snap frozen in liquid nitrogen. Using TRIzol (Life technologies ), total RNA was extracted from brain tissue. Ribosomal RNA (rRNA) was removed before sequencing by Epicentre Ribo-zeroTM rRNA Removal Kit.
RNA libraries were prepared for sequencing using standard Illumina protocols

value: 4.5 years

value: Cerebellum


title: Cerebellum, Z-XN-3

description: Zang Yakow


title: GBM1_1_5

description: library of 192 cells

value: Glioblastoma multiforme


title: D135N3


title: Pseudo-stem, MB_C_Rep3

city: Bandung

first: Popi

last: Septiani

state: West Java

address: jl. Ganeca 10

department: School of Life Science and Technology

country: Indonesia

institute: Institut Teknologi Bandung

description: Control replicate 3

data_processing:
Raw paired-end reads were examined for a possible low base score, Illumina adapter and PCR contaminations using FastQC
Clean reads were mapped against genome reference using TopHat-Cufflink pipeline (Trapnell et al. 2013)
Reads that aligned to annotated loci were quantified and normalized with FPKM value using Cufflinks V2.2.1 (Trapnell et al. 2010)
Genome_build: Musa acuminata DH Pahang V2 (https://banana-genome-hub.southgreen.fr/sites/banana-genome-hub.southgreen.fr/files/data/fasta/version2/musa_acuminata_v2_pseudochromosome.fna) and Musa balbisiana PKW V1 (https://banana-genome-hub.southgreen.fr/sites/banana-genome-hub.southgreen.fr/files/data/fasta/pkw/PKW_pseudochromosome.fa)
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample

organism: Musa balbisiana

source_name: pseudo-stem

growth_protocol: All cultivars were from the two-week-old plants with 2 – 4 leaves and were grown in a sterile 1:2:1 of soil : burnt husk : NPK-fertilizer media. The plants were grown for another three months in a greenhouse with temperature of 20 - 32°C, 50 % - 80 % humidity, and 16h light and 8h dark.

extract_protocol:
Total RNA were extracted from the pseudostem using the RNA isolation method according to Cordeiro et al. (2008)
RNA libraries were prepared for sequencing using standard Illumina protocols

treatment_protocol: Each cultivar was treated in two conditions, ie. control and inoculated. Controls are cultivars without Rsc inoculation. Inoculated are cultivars with Rsc inoculation containing 10e7 cfu/ml in 2.5 ml  suspension injected into the pseudostem of banana.

tag: cultivar

value: Klutuk (BB group)

value: control (no inoculation)

tag: time

value: 3 months


title: Pseudo-stem, MB_I_Rep1

description: Inoculated replicate 1

value: Rsc inoculation

value: 7 days after inoculation


title: Liver, LD Tun 3

source_name: Liver tissues

tag: gender

value: male

value: Liver

tag: agent

value: tunicamycin

value: 10 weeks


title: Cerebrum, Z-DN-2

description: Zang Yakow

source_name: Cerebrum

value: Cerebrum


title: Pseudo-stem, MB_I_Rep2

description: Inoculated replicate 2

tag: treatment


title: Pseudo-stem, MB_C_Rep2

description: Control replicate 2


title: Cerebellum, H-XN-3

description: Sanjiang Cattle

organism: Bos taurus


title: Cerebrum, Z-DN-1

description: Zang Yakow


title: GBM2_GBM_1_5

description: library of 192 cells

value: Brain


title: Cerebellum, H-XN-1

description: Sanjiang Cattle


title: Cerebellum, H-XN-2

description: Sanjiang Cattle


title: Pseudo-stem, MB_C_Rep1

description: Control replicate 1


title: Cerebrum, H-DN-3

description: Sanjiang Cattle


title: gAF_worms_PolyP_rep_1

value: adult


title: Pseudo-stem, MA_I_Rep2

description: Inoculated replicate 2

organism: Musa acuminata

value: Mas Kirana (AA group)


title: Cerebellum, Z-XN-2

description: Zang Yakow


title: Cerebellum, M-XN-3

description: Leiwuqi Yak

organism: Bos grunniens


title: control mimic-tranfected macrophages in the presence of anti-IL-6 Ab #3

city: Kamakura

first: Satoshi

last: Kondo

state: Kanagawa

address: 10-1 tebiro 6-chome

country: Japan

institute: Toray Industries,Inc.

description: Gene expression in control mimic-trasnfected and anti-IL-6 Ab-treated macrophages

hyb_protocol: Hybridized for 16 h at 37 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..

scan_protocol: 3D-Gene Scanner ((Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction(Toray Industries Inc., Tokyo, Japan).

data_processing: The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95% confidence intervals.

source_name: mouse bone marrow derived macrophages #3

label_protocol: Amino Allyl aRNA was synthesis by Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion#1753). CyeDye Coupling and fragmentation were performed as the protocol supplied by TORAY Industries, Inc..

extract_protocol: RNA samples were extracted from cells using RNesy mini kit (Qiagen).

treatment_protocol: Control or miR192 mimic was transfected into mouse bone marrow-derived macrophages, and 24hrs later those cells were stimulated with R848 for additional 24hrs together with control or anti-IL-6 Ab in vitro.

tag: transfected rna

value: control mimic

tag: treated ab

value: anti-IL-6 Ab


title: Pseudo-stem, MA_I_Rep1

description: Inoculated replicate 1


title: Cerebrum, H-DN-2

description: Sanjiang Cattle

value: 4.5 years

tag: tissue


title: GBM1_2_6

description: library of 192 cells

tag: diagnosis

value: Glioblastoma multiforme

value: Temporal lobe


title: miR192 mimic-tranfected macrophages in the presence of anti-IL-6 Ab #4

description: Gene expression in miR192 mimic-trasnfected ad anti-IL-6 Ab-treated macrophages

source_name: mouse bone marrow derived macrophages #4

value: miR192 mimic


title: Pseudo-stem, MA_C_Rep3

description: Control replicate 3

value: control (no inoculation)

value: 3 months


title: Cerebrum, H-DN-1

description: Sanjiang Cattle


title: Cerebrum, Z-DN-3

description: Zang Yakow


title: Cerebellum, M-XN-1

description: Leiwuqi Yak

value: Cerebellum


title: 16S rRNA-seq Rag1‒/‒ and RPS feces (abx)

description:
RPS_abx
Fecal DNA

data_processing:
Library strategy: 16S rRNA-seq
[RNA-seq] De-multiplexed raw data files from the sequencing core were analyzed for quality control using FastQC (Babraham Bioinformatics). Reads were mapped (STAR aligner) to the mm10 assembly of the Mus musculus genome and filtered for uniquely mapped reads. Quantitated relative mRNA expression levels (normalized read counts) were calculated based on exon regions using STAR and the mm10 reference genome annotations.
[16S rRNA-seq] Sequence analyses were performed using QIIME v.1.9.0. After checking the quality of the sequenced reads, 8 nucleotides (nt) barcodes were extracted from both forward and reverse reads to generate a barcode library. Forward and reverse reads were then joined, and sequence libraries were split based on their corresponding barcodes. We used an open reference operational taxonomic unit (OTU) picking strategy to select OTUs (with 97% identity threshold). Taxonomy was assigned based on the Greengenes reference database. A taxonomic table for each taxonomic level was generated based on the OTU table.
Genome_build: mm10 (GRCm38)
Supplementary_files_format_and_content: [RNA-seq] Colon_counts.txt: Tab-delimited text file includes count values for each sample.
Supplementary_files_format_and_content: [16S rRNA-seq] *_table.from_biom_w_taxonomy.txt: Tab-delimited text files include count values for each sample.

organism: feces metagenome

source_name: Feces

extract_protocol:
For microbiome analyses, fecal DNA was isolated and amplified with Illumina Miseq compatible primers, targeting the 16S rRNA gene V4–V5 region.
Amplicons were purified by QIAquick Gel extraction kit (Qiagene, Madison, WI) and quantified by Qubit 2.0 Fluorometer (Invitrogen, Grand Island, NY) and Kapa SYBR fast qPCR kit (Kapa Biosystems, Inc., Woburn, MA). Equal amounts of amplicons were pooled with 10% of Phix control to generate the DNA library.

tag: mouse strain

tag: mouse genotype/variation

value: Feces

tag: gavage

value: Rag1-/- Selplg-/- feces

value: Antibiotics


title: Cerebellum, M-XN-2

description: Leiwuqi Yak


title: Pseudo-stem, MA_C_Rep2

description: Control replicate 2


title: miR192 mimic-tranfected macrophages in the presence of control Ab #2

description: Gene expression in miR192 mimic-trasnfected and control Ab-treated macrophages

source_name: mouse bone marrow derived macrophages #2

value: control Ab


title: B. subtilis_DAS4_rep2

city: Greifswald

first: Ulrike

last: Mäder

address: F.-L.-Jahn-Str. 15A

department: Functional Genomics

institute: University Medicine Greifswald

hyb_protocol: 600 ng of Cy5-labeled cDNA and 600 ng of Cy3-labeled cDNA were hybridized to the microarray following Agilent’s hybridization, washing and scanning protocol (Two-Color Microarray-based Gene Expression Analysis, version 5.5).

scan_protocol: The microarray was scanned using an Agilent Microarray Scanner G2505C (Agilent Technologies).

data_processing:
Data were extracted using the Agilent Feature Extraction software (version 10.5, Agilent Technologies).
The normalization/ dye bias correction method as defined by the Agilent Feature Extraction protocol for 2 color gene expression arrays (protocol GE2_105_Dec08) is: Linear and Lowess.  The normalization/ dye bias correction method is: Linear and Lowess.

organism: Bacillus subtilis

source_name: B. subtilis_before DAS4 treatment

label_protocol: For cDNA synthesis, 5 µg of total RNA were mixed with random primers (FairPlay III Microarray Labeling Kit) and spike-ins (One-Color RNA Spike-In Kit, Agilent Technologies) and incubated at 70°C for 10 min followed by 5 min incubation on ice. Then, first-strand Master Mix, Actinomycin D (final concentration 40 µg/ml) and AffinityScript HC Reverse Transcriptase were added. The reaction was incubated for 60 min at room temperature and for 60 min at 42°C. After hydrolyzing the RNA, cDNA was precipitated overnight at -20°C. NHS-ester dye coupling (CyDye Mono-Reactive Dye, GE Healthcare) and purification of labeled cDNA were performed according to the FairPlay III instructions. cDNA and Cy-dye concentrations were quantified by means of a NanoDrop spectrophotometer.

growth_protocol: B. subtilis 168 was grown under vigorous agitation at 37 °C in Belitsky minimal medium (BMM) described before (Stülke et al., 1993; PMID 8245830).

extract_protocol: Total RNA was isolated by the acis phenol method as described (Chi et al., 2011; PMID 21749987).

treatment_protocol: At OD500 of 0.4, cells were treated for 30 minutes with 2.5 mM NaHS, 90 µM allicin or 92 µM DAS4.

tag: strain

value: Bacillus subtilis subsp. subtilis str. 168

value: none; before treatment

source_name: B. subtilis_DAS4 treated

value: 92 µM DAS4


title: Cerebrum, M-DN-3

description: Leiwuqi Yak

value: Cerebrum


title: Cerebrum, M-DN-2

description: Leiwuqi Yak


title: B. subtilis_DAS4_rep1


title: 16S rRNA-seq Rag1‒/‒ and RPS feces

description:
RPS
Fecal DNA


title: Cerebrum, M-DN-1

description: Leiwuqi Yak


title: sea_urchin_blastula

status: Public on Nov 17 2019

city: beijing

first: jiang

last: liu

address: NO.1 Beichen West Road, Chaoyang District

institute: Beijing Institute of Genomics, Chinese Academy of Sciences

description: 2 replicates;replicate1[sea_urchin_blastula_rep1_*.fastq.gz,sea_urchin_blastula_rep2_*.fastq.gz];replicate2[sea_urchin_blastula_rep3_*.fastq.gz,sea_urchin_blastula_rep4_*.fastq.gz]

data_processing:
Adapters and low-quality reads were trimed with Trimmomatic
Reads alignment were performed with Bismark with default settings
duplicates were removed by deduplicate_bismark function of Bismark, and overlapped part of paired-end reads were removed by clipOverlap of bamUtil_1.0.9
methylation level were extracted with mpileup function of Samtools (v0.1.19)
Genome_build: sea_anemone[ASM20922v1];honey_bee[GCA_000002195.1];sea_urchin[Spur_3.1];ciona[CSAV2.0]
Supplementary_files_format_and_content: Format: bed; content: Methylation level for each CpG site plus 2 columns representing the numbers of Cs and Ts

organism: Strongylocentrotus purpuratus

source_name: sea_urchin blastula

extract_protocol:
The gametes and embryos were frozen at 70 below zero or subjected to immediate genomic extraction procedures.  Genomic DNA was extracted by QIAamp DNA Mini Kit(QIAGEN) following the protocol of DNA Purification from Tissues with the cell lysis time adjusted depending on different samples.
Genomic DNA spiked with 0.5％ unmethylated Lambda DNA (Promega) was sonicated into 100-500bp. Fragmented DNA was then subjected to end repair,dA tailing, ligation with the end repair enzyme mix (NEB),  Klenow 3´-5´ exo- (NEB) and  T4 DNA ligase (NEB), respectively. Adapter-ligated DNA was recovered by 2％ agarose gel electrophoresis, and bisulfite conversion was performed using the EZ DNA methylation-Gold kit (Zymo Research) according to the instruction manual. Bisulfite-treated DNA was amplified with KAPA HiFi HotStart Uracil+ ReadyMix. Amplified DNA  was recovered by 2％ agarose gel electrophoresis.

tag: cell type

value: blastula

tag: developmental stage

value: embryo


title: sea_urchin_oocyte

source_name: sea_urchin oocyte

value: oocyte

value: gamete


title: sea_urchin_sperm

description: 2 replicates;replicate1[sea_urchin_sperm_rep1_*.fastq.gz;sea_urchin_sperm_rep3_*.fastq.gz];replicate2[sea_urchin_sperm_rep2_*.fastq.gz]

source_name: sea_urchin sperm

value: sperm


title: sea_urchin_cleavage

description: 2 replicates;replicate1[sea_urchin_cleavage_rep1_*.fastq.gz;sea_urchin_cleavage_rep3_*.fastq.gz];replicate2[sea_urchin_cleavage_rep2_*.fastq.gz];

source_name: sea_urchin cleavage

value: cleavage


title: single neuron, striatum, D11_1000701502-OGC22-sal_1_C7

city: San Francisco

first: Stephen

last: Quake

address: 499 Illinois St

institute: Chan-Zuckerberg Biohub

description: single neuron isolated from mouse striatum

data_processing:
Alignment (STAR v2.4.0) to mm10 mouse genome
Gene counting (HTSeq v0.11.1)
Normalization (log10 cpm + 1)
Removal of low quality cells (having < 1000 genes detected and <10^5 counts)
Genes scaled using Seurat v2.3.4 ScaleData
Genome_build: mm10
Supplementary_files_format_and_content: striatum_final.rds, striatum_raw_counts.csv

source_name: striatum

extract_protocol:
Generation of D1-tdTom/D2-GFP, and D1-tomato BAC reporter mice was previously described(Gokce et al., 2016). All lines were maintained by backcrossing to C57Bl/6J. All other experiments were conducted on 5-7 week old male C57/Bl6 mice from Jackson Labs. Mice were weaned at ~21 days of age and housed in groups of 2-5 on a 12-hour light/dark cycle (lights on 0700-1900h), with free access to food. All procedures conformed to the National Institutes of Health Guidelines for the Care and Use of Laboratory Animals and were approved by the Stanford University. Parasagittal slices (350 μm) containing striatum were prepared using standard  procedures. Briefly, mice were anesthetized with isoflurane and decapitated,  brains were quickly removed and placed in ice-cold low-sodium/high-sucrose dissecting solution. Slices were cut by adhering the lateral surface of the brain to the stage of a Leica vibroslicer, and placed in ice-cold hibernate-A solution (Gibco, Themo Fisher). The striatum was dissected from each slice, and the tissue was dissociated using papain-based dissociation. Briefly, striatal tissue was incubated with frequent agitation at 30°C for 20 min 2mg/ml papain solution in Hibernate-A, followed by washing with hibernate-A including 1mg/ml Ovomucoid protease inhibitor (Sigma). The striatal slices were triturated briefly using fire polished glass Pasteur pipettes until a single-cell suspension was obtained. The suspension was cleaned from small debris by centrifuging for 3 min at 450g through a three-step density gradient of Percoll (Sigma), top layer containing small debris was discarded and remaining cells were pelleted for 5  min at 550g. The pellet was resuspended in 0.1 ml of hibernate A with DAPI (4’,6-diamidino-2-phenylindole, 1:4000 dilution; Sigma) to label dead cells(Gokce et al., 2016). Striatal tissue was kept in ice-cold solution throughout the processing except during the 20 min dissociation with papain at 30°C. Administrative Panel on Laboratory Animal Care. The dissociated cell suspension from adult D1-tdTom/D2-GFP mice was passed through a 70-μm cell strainer (BD Biosciences). Viable cells (DAPI negative) were sorted by FACS (Aria II; BD Biosciences). For specific enrichment of genetically labeled cell types, fluorescent striatal cells were analyzed to determine optimal parameters and gating for FACS. Finally, test sorted cells were imaged on confocal microscope as described previously(Gokce et al., 2016). Cells were sorted into ice-cold hibernate-A and pelleted by centrifugation for 5 min at 300g. Single cells were sorted into 96 well plates filled with 4 μl lysis buffer composed of 0.05% Triton X-100 (Sigma) and, ERCC spike-ins (1:24000000 dilution), 2.5 μM oligo-dT, 2.5 mM dNTP and 2U/μl of recombinant RNase inhibitor (Clontech) then spun down and frozen at -80°C. Plates were thawed and libraries prepared as described below.
96-well plates with sorted single cells were incubated for 3min at 72°C and immediately placed on ice. To perform reverse transcription (RT) we added each well a mix of 0.59μl H2O, 0.5μl SMARTScribe™ Reverse Transcriptase (Clontech), 2μl 5x First Strand buffer, 0.25μl Recombinant RNase Inhibitor (Clontech), 2μl Betaine (5M Sigma), 0.5μl DTT (100mM) 0.06μl MgCL2 (1M Sigma), 0.1μl Template-switching oligos (TSO) (100μM AAGCAGTGGTATCAACGCAGAGTACrGrG+G). Next reaction RT reaction mixes were incubated at 42°C for 90 min followed by 70°C for 5 min. Pre amplification of cDNA done by adding 12.5μl KAPA HiFi Hotstart 2x (KAPA Biosystems), 2.138μl H2O, 0.25μl ISPCR primers (10 μM, 5′-AAGCAGTGGTATCAACGCAGAGT-3), 0.1125μl Lambda Exonuclease under the following conditions: 37°C for 30 min, 95°C for 3 min, 21 cycles of (98°C for 20 sec, 67°C for 15 sec, 72°C for 4 min), final extension at 72°C for 5 min. Cells were then cleaned using 2 rounds of AMPure bead (Beckman-Coulter) cleanup at a 0.8:1 ratio of beads to PCR product. The cleaned cDNA was then tagmented using the Nextera XT DNA kit and following the included instructions, using 11 cycles of PCR to amplify the tagmented cDNA (Illumina). Tagmented cDNA was then pooled and analyzed with a Bioanalyzer 2100 High Sensitivity DNA kit (Agilent) to assure that >80%  of the product was in the 200-1000bp range. This process was repeated separately for each animal. Libaries were sequenced using 2x75 reads base pairs (bp) paired-end on Illumina HiSeq 2000 or NextSeq to a target depth of 1x10e6 reads/sample.

tag: nreads

tag: library

tag: subcluster

value: striatum

value: male


title: single neuron, striatum, C11_1000701806-OGC18-sal_1_H1


title: single neuron, striatum, A1_1000701002-OGC14-sal_1_N6

value: NAcc


title: single neuron, striatum, H5_1000701805-OGC18-sal_1_C23

value: ICj


title: single neuron, striatum, E5_1000701806-OGC18-sal_1_I2

value: MedShell


title: single neuron, striatum, C10_1000701806-OGC18-sal_1_G1


title: single neuron, striatum, F12_1000701702-OGC17-sal_1_E16


title: single neuron, striatum, C9_1000701702-OGC17-sal_1_N13


title: single neuron, striatum, F3_1000701805-OGC18-sal_1_J21


title: B. subtilis_NaHS_rep2

source_name: B. subtilis_before NaHS treatment

source_name: B. subtilis_NaHS treated

value: 2.5 mM NaHS


title: B. subtilis_allicin_rep2

source_name: B. subtilis_before allicin treatment

source_name: B. subtilis_allicin treated

value: 90 µM allicin


title: B. subtilis_NaHS_rep1

value: Bacillus subtilis subsp. subtilis str. 168


title: control mimic-tranfected macrophages in the presence of control Ab #1

description: Gene expression in control mimic-trasnfected and control Ab-treated macrophages

source_name: mouse bone marrow derived macrophages #1

value: control mimic

value: control Ab


title: single neuron, striatum, H4_1000701002-OGC14-sal_1_H11


title: single neuron, striatum, H5_1000701002-OGC14-sal_1_I11


title: B. subtilis_allicin_rep1

value: none; before treatment


title: single neuron, striatum, B2_1000701502-OGC22-sal_1_F5


title: single neuron, striatum, E10_1000701002-OGC14-sal_1_P9


title: Pseudo-stem, MA_C_Rep1

description: Control replicate 1

source_name: pseudo-stem

tag: cultivar

value: Mas Kirana (AA group)

value: control (no inoculation)

tag: time

tag: age

value: 3 months


title: single neuron, striatum, B5_1000701002-OGC14-sal_1_L7

tag: gender


title: single neuron, striatum, D1_1000701805-OGC18-sal_1_A20


title: single neuron, striatum, H9_1000701806-OGC18-sal_1_I4


title: single neuron, striatum, H4_1000701702-OGC17-sal_1_E17


title: single neuron, striatum, E9_1000701502-OGC22-sal_1_J7


title: single neuron, striatum, H2_1000701502-OGC22-sal_1_M8

value: unknown


title: single neuron, striatum, F10_1000701002-OGC14-sal_1_K10


title: single neuron, striatum, F10_1000701702-OGC17-sal_1_C16


title: single neuron, striatum, H3_1000701805-OGC18-sal_1_A23


title: single neuron, striatum, A3_1000701502-OGC22-sal_1_N4


title: single neuron, striatum, B8_1000701806-OGC18-sal_1_K24


title: single neuron, striatum, B5_1000701805-OGC18-sal_1_M18


title: single neuron, striatum, G9_1000701805-OGC18-sal_1_K22


title: single neuron, striatum, D12_1000701002-OGC14-sal_1_F9


title: single neuron, striatum, B7_1000701702-OGC17-sal_1_P12


title: single neuron, striatum, A10_1000701806-OGC18-sal_1_A24


title: single neuron, striatum, D6_1000701805-OGC18-sal_1_F20


title: single neuron, striatum, D11_1000701806-OGC18-sal_1_C2


title: single neuron, striatum, B12_1000701805-OGC18-sal_1_D19


title: single neuron, striatum, D3_1000701502-OGC22-sal_1_L6


title: single neuron, striatum, C5_1000701702-OGC17-sal_1_J13


title: single neuron, striatum, H5_1000701502-OGC22-sal_1_O8


title: single neuron, striatum, H3_1000701806-OGC18-sal_1_E4


title: single neuron, striatum, E2_1000701805-OGC18-sal_1_M20


title: single neuron, striatum, C3_1000701806-OGC18-sal_1_A1


title: single neuron, striatum, B4_1000701806-OGC18-sal_1_G24


title: single neuron, striatum, F1_1000701502-OGC22-sal_1_L7


title: single neuron, striatum, F9_1000701702-OGC17-sal_1_B16


title: single neuron, striatum, E4_1000701702-OGC17-sal_1_A15


title: single neuron, striatum, F1_1000701002-OGC14-sal_1_C10


title: single neuron, striatum, C6_1000701702-OGC17-sal_1_K13


title: single neuron, striatum, F9_1000701806-OGC18-sal_1_E3

value: vClusters


title: single neuron, striatum, F3_1000701702-OGC17-sal_1_L15


title: single neuron, striatum, H10_1000701805-OGC18-sal_1_H23


title: single neuron, striatum, G1_1000701805-OGC18-sal_1_C22


title: single neuron, striatum, E12_1000701805-OGC18-sal_1_G21


title: single neuron, striatum, C5_1000701805-OGC18-sal_1_I19


title: single neuron, striatum, F7_1000701806-OGC18-sal_1_C3


title: single neuron, striatum, A9_1000701502-OGC22-sal_1_B5


title: single neuron, striatum, G5_1000701702-OGC17-sal_1_J16


title: single neuron, striatum, G11_1000701806-OGC18-sal_1_B4


title: single neuron, striatum, H7_1000701502-OGC22-sal_1_A9


title: single neuron, striatum, F6_1000701502-OGC22-sal_1_O7


title: single neuron, striatum, C9_1000701806-OGC18-sal_1_F1


title: single neuron, striatum, B7_1000701806-OGC18-sal_1_J24


title: single neuron, striatum, F1_1000701702-OGC17-sal_1_J15


title: single neuron, striatum, C1_1000701806-OGC18-sal_1_O24


title: single neuron, striatum, F12_1000701806-OGC18-sal_1_G3


title: single neuron, striatum, D6_1000701002-OGC14-sal_1_A9


title: single neuron, striatum, F9_1000701002-OGC14-sal_1_J10


title: single neuron, striatum, D10_1000701806-OGC18-sal_1_B2


title: single neuron, striatum, D9_1000701806-OGC18-sal_1_A2


title: single neuron, striatum, D1_1000701502-OGC22-sal_1_J6


title: single neuron, striatum, A3_1000701806-OGC18-sal_1_J23


title: single neuron, striatum, F4_1000701806-OGC18-sal_1_P2


title: single neuron, striatum, B2_1000701805-OGC18-sal_1_J18


title: single neuron, striatum, D7_1000701806-OGC18-sal_1_O1


title: single neuron, striatum, G8_1000701502-OGC22-sal_1_J8


title: single neuron, striatum, C6_1000701805-OGC18-sal_1_J19


title: single neuron, striatum, D8_1000701002-OGC14-sal_1_C9


title: single neuron, striatum, G3_1000701502-OGC22-sal_1_F8


title: single neuron, striatum, C7_1000701002-OGC14-sal_1_H8


title: single neuron, striatum, G3_1000701806-OGC18-sal_1_J3


title: single neuron, striatum, E9_1000701805-OGC18-sal_1_D21


title: single neuron, striatum, E4_1000701806-OGC18-sal_1_H2


title: single neuron, striatum, A7_1000701805-OGC18-sal_1_C18


title: single neuron, striatum, G5_1000701806-OGC18-sal_1_L3


title: single neuron, striatum, B1_1000701805-OGC18-sal_1_I18


title: single neuron, striatum, A12_1000701805-OGC18-sal_1_H18


title: single neuron, striatum, F12_1000701805-OGC18-sal_1_B22


title: single neuron, striatum, B12_1000701502-OGC22-sal_1_M5


title: single neuron, striatum, H10_1000701702-OGC17-sal_1_K17


title: single neuron, striatum, G5_1000701805-OGC18-sal_1_G22


title: single neuron, striatum, G2_1000701502-OGC22-sal_1_E8


title: single neuron, striatum, B3_1000701502-OGC22-sal_1_G5


title: single neuron, striatum, E8_1000701805-OGC18-sal_1_C21


title: single neuron, striatum, H8_1000701702-OGC17-sal_1_I17


title: single neuron, striatum, B9_1000701502-OGC22-sal_1_K5


title: single neuron, striatum, A6_1000701702-OGC17-sal_1_C12


title: single neuron, striatum, E2_1000701806-OGC18-sal_1_F2


title: single neuron, striatum, G6_1000701806-OGC18-sal_1_M3


title: single neuron, striatum, B9_1000701702-OGC17-sal_1_B13


title: single neuron, striatum, H10_1000701806-OGC18-sal_1_J4


title: single neuron, striatum, C12_1000701805-OGC18-sal_1_P19


title: single neuron, striatum, D8_1000701806-OGC18-sal_1_P1


title: single neuron, striatum, E10_1000701702-OGC17-sal_1_G15


title: single neuron, striatum, G11_1000701805-OGC18-sal_1_M22


title: single neuron, striatum, H11_1000701805-OGC18-sal_1_I23


title: single neuron, striatum, D7_1000701002-OGC14-sal_1_B9


title: single neuron, striatum, C9_1000701805-OGC18-sal_1_M19


title: single neuron, striatum, F8_1000701806-OGC18-sal_1_D3


title: single neuron, striatum, A12_1000701002-OGC14-sal_1_H7


title: single neuron, striatum, C5_1000701806-OGC18-sal_1_C1


title: single neuron, striatum, F5_1000701002-OGC14-sal_1_F10


title: single neuron, striatum, C1_1000701702-OGC17-sal_1_F13


title: single neuron, striatum, H10_1000701502-OGC22-sal_1_D9


title: single neuron, striatum, G9_1000701002-OGC14-sal_1_B11


title: Pat15_WM_1_3

description: library of 192 cells

data_processing:
For image aquisition, intensity extraction and basecalling HiSeq Control Software  2.0.2, RTA 2.4.11 / Recipe Fragment 2.0.0.2 was used. Conversion of bcl2fastq files was performed using bcl2fastq 2.17.1.14
Paired end reads were aligned to the transcriptome using bwa (version 0.6.2-r126) with default parameters. The transcriptome contained all gene models based on the mouse ENCODE VM9 release downloaded from the UCSC genome browser comprising 57,207 isoforms derived from 57,207 gene loci with 57,114 isoforms mapping to fully annotated chromosomes (1 to 19, X, Y, M). All isoforms of the same gene were merged to a single gene locus. Furthermore, gene loci overlapping by >75% were merged to larger gene groups. This procedure resulted in 34,111 gene groups.
The right mate of each read pair was mapped to the ensemble of all gene loci and to the set of 92 ERCC spike-ins in sense direction. Reads mapping to multiple loci were discarded. The left read contains the barcode information: the first six bases correspond to the cell specific barcode followed by six bases representing the unique molecular identifier (UMI). The remainder of the left read contains a poly(T) stretch. The left read was not used for quantification. For each cell barcode, the number of UMIs per transcript was counted and aggregated across all transcripts derived from the same gene locus.
Based on binomial statistics, the number of observed UMIs was converted into transcript counts (Gruen et al., 2014).
Genome_build: ENCODE VM9
Supplementary_files_format_and_content: CSV files, columns represent each cell barcode (total barcodes used = 192), rows represent the geneid and the values in the file are the quantified number of transcripts.

source_name: Human brain surgery sample

extract_protocol:
Fresh tissue was dissected by removing the meninges. Single-cell suspesions were generated with a dounce potter followed by passing through a cell strainer. Myelin was removed using percoll gradient centrifugation.
As described in mCEL-Seq2 protocol (Hashimshony et al. 2016 and Herman et al. 2018)
Adapted from TruSeq Small RNA Library Preparation Protocol

value: Brain

value: Control

tag: region

value: Temporal lobe


title: single neuron, striatum, H2_1000701805-OGC18-sal_1_P22

value: striatum

value: male


title: single neuron, striatum, D5_1000701502-OGC22-sal_1_M6


title: single neuron, striatum, G1_1000701806-OGC18-sal_1_H3


title: single neuron, striatum, F2_1000701806-OGC18-sal_1_N2


title: single neuron, striatum, B11_1000701805-OGC18-sal_1_C19

value: MedShell


title: single neuron, striatum, D12_1000701502-OGC22-sal_1_D7


title: single neuron, striatum, G12_1000701806-OGC18-sal_1_C4


title: single neuron, striatum, B4_1000701002-OGC14-sal_1_K7


title: single neuron, striatum, E6_1000701805-OGC18-sal_1_A21

value: ICj


title: single neuron, striatum, G10_1000701806-OGC18-sal_1_A4


title: single neuron, striatum, E1_1000701502-OGC22-sal_1_E7


title: single neuron, striatum, C10_1000701702-OGC17-sal_1_O13


title: single neuron, striatum, E12_1000701502-OGC22-sal_1_K7


title: single neuron, striatum, B10_1000701806-OGC18-sal_1_M24


title: single neuron, striatum, G11_1000701002-OGC14-sal_1_C11


title: single neuron, striatum, E5_1000701805-OGC18-sal_1_P20


title: single neuron, striatum, F6_1000701002-OGC14-sal_1_G10


title: single neuron, striatum, D12_1000701805-OGC18-sal_1_K20


title: single neuron, striatum, C4_1000701806-OGC18-sal_1_B1


title: single neuron, striatum, C3_1000701805-OGC18-sal_1_G19


title: single neuron, striatum, E1_1000701702-OGC17-sal_1_N14


title: single neuron, striatum, B7_1000701502-OGC22-sal_1_I5


title: single neuron, striatum, F10_1000701502-OGC22-sal_1_C8

value: NAcc


title: single neuron, striatum, F3_1000701002-OGC14-sal_1_E10


title: single neuron, striatum, A9_1000701702-OGC17-sal_1_F12


title: single neuron, striatum, H1_1000701805-OGC18-sal_1_O22


title: single neuron, striatum, G12_1000701702-OGC17-sal_1_A17


title: single neuron, striatum, A5_1000701002-OGC14-sal_1_B7


title: single neuron, striatum, H2_1000701702-OGC17-sal_1_C17


title: single neuron, striatum, G7_1000701806-OGC18-sal_1_N3


title: single neuron, striatum, A3_1000701002-OGC14-sal_1_P6


title: single neuron, striatum, C10_1000701002-OGC14-sal_1_J8


title: single neuron, striatum, E12_1000701002-OGC14-sal_1_B10


title: single neuron, striatum, F5_1000701806-OGC18-sal_1_A3


title: single neuron, striatum, G2_1000701806-OGC18-sal_1_I3


title: single neuron, striatum, A8_1000701806-OGC18-sal_1_O23


title: single neuron, striatum, H8_1000701502-OGC22-sal_1_B9


title: single neuron, striatum, H2_1000701806-OGC18-sal_1_D4


title: single neuron, striatum, E5_1000701002-OGC14-sal_1_K9


title: single neuron, striatum, C3_1000701502-OGC22-sal_1_P5


title: single neuron, striatum, G2_1000701702-OGC17-sal_1_G16


title: single neuron, striatum, D6_1000701702-OGC17-sal_1_G14


title: single neuron, striatum, C7_1000701702-OGC17-sal_1_L13


title: single neuron, striatum, E7_1000701805-OGC18-sal_1_B21


title: single neuron, striatum, D3_1000701806-OGC18-sal_1_L1


title: single neuron, striatum, B11_1000701502-OGC22-sal_1_L5


title: single neuron, striatum, F10_1000701805-OGC18-sal_1_A22


title: single neuron, striatum, F3_1000701806-OGC18-sal_1_O2


title: single neuron, striatum, H10_1000701002-OGC14-sal_1_M11


title: single neuron, striatum, D4_1000701806-OGC18-sal_1_M1


title: single neuron, striatum, F1_1000701806-OGC18-sal_1_M2


title: single neuron, striatum, D8_1000701502-OGC22-sal_1_P6


title: single neuron, striatum, B7_1000701002-OGC14-sal_1_N7


title: single neuron, striatum, B3_1000701805-OGC18-sal_1_K18


title: single neuron, striatum, G7_1000701805-OGC18-sal_1_I22


title: single neuron, striatum, H2_1000701002-OGC14-sal_1_F11


title: single neuron, striatum, G9_1000701806-OGC18-sal_1_P3


title: single neuron, striatum, H11_1000701806-OGC18-sal_1_K4


title: single neuron, striatum, H9_1000701002-OGC14-sal_1_L11


title: single neuron, striatum, F11_1000701002-OGC14-sal_1_L10


title: single neuron, striatum, C6_1000701806-OGC18-sal_1_D1


title: single neuron, striatum, B1_1000701806-OGC18-sal_1_D24


title: single neuron, striatum, C8_1000701806-OGC18-sal_1_E1


title: single neuron, striatum, A7_1000701002-OGC14-sal_1_D7


title: single neuron, striatum, B9_1000701806-OGC18-sal_1_L24


title: single neuron, striatum, F2_1000701702-OGC17-sal_1_K15


title: single neuron, striatum, A11_1000701702-OGC17-sal_1_H12


title: single neuron, striatum, D12_1000701806-OGC18-sal_1_D2


title: single neuron, striatum, G7_1000701702-OGC17-sal_1_L16


title: single neuron, striatum, B10_1000701002-OGC14-sal_1_A8


title: single neuron, striatum, D3_1000701805-OGC18-sal_1_C20


title: single neuron, striatum, A6_1000701806-OGC18-sal_1_M23


title: single neuron, striatum, B8_1000701502-OGC22-sal_1_J5


title: single neuron, striatum, G11_1000701502-OGC22-sal_1_L8


title: single neuron, striatum, H6_1000701806-OGC18-sal_1_G4


title: single neuron, striatum, A2_1000701502-OGC22-sal_1_M4


title: single neuron, striatum, D2_1000701502-OGC22-sal_1_K6


title: single neuron, striatum, A6_1000701002-OGC14-sal_1_C7


title: single neuron, striatum, F6_1000701702-OGC17-sal_1_O15


title: single neuron, striatum, D12_1000701702-OGC17-sal_1_M14


title: single neuron, striatum, G3_1000701702-OGC17-sal_1_H16


title: single neuron, striatum, E11_1000701702-OGC17-sal_1_H15


title: single neuron, striatum, D6_1000701806-OGC18-sal_1_N1


title: single neuron, striatum, D2_1000701002-OGC14-sal_1_N8


title: single neuron, striatum, A4_1000701806-OGC18-sal_1_K23


title: single neuron, striatum, D9_1000701805-OGC18-sal_1_H20


title: single neuron, striatum, D2_1000701805-OGC18-sal_1_B20


title: single neuron, striatum, B8_1000701002-OGC14-sal_1_O7


title: single neuron, striatum, H6_1000701702-OGC17-sal_1_G17


title: single neuron, striatum, D7_1000701502-OGC22-sal_1_O6


title: single neuron, striatum, D1_1000701806-OGC18-sal_1_J1


title: single neuron, striatum, H7_1000701805-OGC18-sal_1_E23


title: single neuron, striatum, G10_1000701702-OGC17-sal_1_O16


title: single neuron, striatum, E7_1000701002-OGC14-sal_1_M9


title: single neuron, striatum, A2_1000701702-OGC17-sal_1_O11


title: single neuron, striatum, C4_1000701702-OGC17-sal_1_I13


title: single neuron, striatum, C10_1000701502-OGC22-sal_1_G6


title: single neuron, striatum, D8_1000701702-OGC17-sal_1_I14


title: single neuron, striatum, C8_1000701502-OGC22-sal_1_E6


title: single neuron, striatum, B6_1000701002-OGC14-sal_1_M7


title: single neuron, striatum, D7_1000701805-OGC18-sal_1_G20


title: single neuron, striatum, G6_1000701805-OGC18-sal_1_H22


title: single neuron, striatum, H9_1000701805-OGC18-sal_1_G23


title: single neuron, striatum, C8_1000701702-OGC17-sal_1_M13


title: single neuron, striatum, H6_1000701805-OGC18-sal_1_D23


title: single neuron, striatum, H4_1000701502-OGC22-sal_1_N8


title: single neuron, striatum, E3_1000701002-OGC14-sal_1_I9


title: single neuron, striatum, D5_1000701805-OGC18-sal_1_E20


title: single neuron, striatum, G12_1000701805-OGC18-sal_1_N22


title: single neuron, striatum, A12_1000701502-OGC22-sal_1_E5


title: single neuron, striatum, C6_1000701502-OGC22-sal_1_C6


title: single neuron, striatum, G8_1000701805-OGC18-sal_1_J22


title: single neuron, striatum, C1_1000701002-OGC14-sal_1_C8


title: single neuron, striatum, A2_1000701002-OGC14-sal_1_O6


title: single neuron, striatum, G5_1000701002-OGC14-sal_1_O10


title: single neuron, striatum, B2_1000701002-OGC14-sal_1_J7


title: single neuron, striatum, F1_1000701805-OGC18-sal_1_H21


title: single neuron, striatum, F8_1000701002-OGC14-sal_1_I10


title: single neuron, striatum, B12_1000701806-OGC18-sal_1_N24


title: single neuron, striatum, C12_1000701806-OGC18-sal_1_I1


title: single neuron, striatum, F2_1000701805-OGC18-sal_1_I21


title: single neuron, striatum, E10_1000701805-OGC18-sal_1_E21


title: single neuron, striatum, E6_1000701002-OGC14-sal_1_L9


title: single neuron, striatum, C12_1000701502-OGC22-sal_1_I6


title: single neuron, striatum, E9_1000701702-OGC17-sal_1_F15


title: single neuron, striatum, H1_1000701002-OGC14-sal_1_E11


title: single neuron, striatum, C4_1000701805-OGC18-sal_1_H19


title: single neuron, striatum, D1_1000701702-OGC17-sal_1_B14


title: single neuron, striatum, G8_1000701806-OGC18-sal_1_O3


title: single neuron, striatum, D10_1000701805-OGC18-sal_1_I20


title: single neuron, striatum, B7_1000701805-OGC18-sal_1_O18


title: single neuron, striatum, A8_1000701002-OGC14-sal_1_E7


title: single neuron, striatum, D11_1000701002-OGC14-sal_1_E9


title: single neuron, striatum, H4_1000701806-OGC18-sal_1_F4


title: single neuron, striatum, C11_1000701502-OGC22-sal_1_H6


title: single neuron, striatum, F7_1000701805-OGC18-sal_1_N21


title: single neuron, striatum, D10_1000701002-OGC14-sal_1_D9


title: single neuron, striatum, H6_1000701002-OGC14-sal_1_J11


title: single neuron, striatum, C2_1000701702-OGC17-sal_1_G13


title: single neuron, striatum, A4_1000701702-OGC17-sal_1_A12


title: single neuron, striatum, C10_1000701805-OGC18-sal_1_N19


title: single neuron, striatum, A6_1000701805-OGC18-sal_1_B18


title: single neuron, striatum, A1_1000701805-OGC18-sal_1_M17


title: single neuron, striatum, F8_1000701805-OGC18-sal_1_O21


title: single neuron, striatum, C12_1000701702-OGC17-sal_1_A14


title: single neuron, striatum, B10_1000701805-OGC18-sal_1_B19


title: single neuron, striatum, D9_1000701502-OGC22-sal_1_A7


title: single neuron, striatum, F4_1000701805-OGC18-sal_1_K21


title: single neuron, striatum, F9_1000701502-OGC22-sal_1_B8


title: single neuron, striatum, F7_1000701702-OGC17-sal_1_P15


title: single neuron, striatum, F2_1000701502-OGC22-sal_1_M7


title: single neuron, striatum, G12_1000701002-OGC14-sal_1_D11


title: single neuron, striatum, F9_1000701805-OGC18-sal_1_P21


title: single neuron, striatum, F4_1000701702-OGC17-sal_1_M15


title: single neuron, striatum, G7_1000701502-OGC22-sal_1_I8


title: single neuron, striatum, E1_1000701805-OGC18-sal_1_L20


title: single neuron, striatum, E2_1000701502-OGC22-sal_1_F7


title: single neuron, striatum, B4_1000701805-OGC18-sal_1_L18


title: single neuron, striatum, G4_1000701806-OGC18-sal_1_K3


title: single neuron, striatum, F8_1000701502-OGC22-sal_1_A8


title: single neuron, striatum, H3_1000701002-OGC14-sal_1_G11


title: single neuron, striatum, A8_1000701805-OGC18-sal_1_D18


title: single neuron, striatum, G7_1000701002-OGC14-sal_1_A11


title: single neuron, striatum, G4_1000701702-OGC17-sal_1_I16


title: single neuron, striatum, C2_1000701805-OGC18-sal_1_F19


title: single neuron, striatum, C5_1000701502-OGC22-sal_1_B6


title: single neuron, striatum, G2_1000701805-OGC18-sal_1_D22


title: single neuron, striatum, D10_1000701702-OGC17-sal_1_K14


title: single neuron, striatum, C2_1000701002-OGC14-sal_1_D8


title: single neuron, striatum, B9_1000701805-OGC18-sal_1_A19


title: single neuron, striatum, C4_1000701502-OGC22-sal_1_A6


title: single neuron, striatum, D4_1000701805-OGC18-sal_1_D20


title: single neuron, striatum, A12_1000701702-OGC17-sal_1_I12


title: single neuron, striatum, E8_1000701002-OGC14-sal_1_N9


title: single neuron, striatum, E1_1000701002-OGC14-sal_1_G9


title: single neuron, striatum, G3_1000701002-OGC14-sal_1_M10


title: single neuron, striatum, F11_1000701702-OGC17-sal_1_D16


title: single neuron, striatum, C11_1000701002-OGC14-sal_1_K8


title: single neuron, striatum, E1_1000701806-OGC18-sal_1_E2


title: single neuron, striatum, H6_1000701502-OGC22-sal_1_P8


title: single neuron, striatum, E11_1000701806-OGC18-sal_1_L2


title: single neuron, striatum, E5_1000701502-OGC22-sal_1_H7


title: single neuron, striatum, A5_1000701702-OGC17-sal_1_B12


title: single neuron, striatum, D10_1000701502-OGC22-sal_1_B7


title: single neuron, striatum, E7_1000701702-OGC17-sal_1_D15


title: single neuron, striatum, E4_1000701805-OGC18-sal_1_O20


title: single neuron, striatum, C8_1000701002-OGC14-sal_1_I8


title: single neuron, striatum, C12_1000701002-OGC14-sal_1_L8


title: single neuron, striatum, E5_1000701702-OGC17-sal_1_B15


title: single neuron, striatum, F7_1000701502-OGC22-sal_1_P7


title: single neuron, striatum, C11_1000701805-OGC18-sal_1_O19


title: single neuron, striatum, G9_1000701502-OGC22-sal_1_K8


title: single neuron, striatum, B3_1000701806-OGC18-sal_1_F24


title: single neuron, striatum, A3_1000701805-OGC18-sal_1_O17


title: single neuron, striatum, E11_1000701805-OGC18-sal_1_F21


title: single neuron, striatum, A1_1000701702-OGC17-sal_1_N11


title: single neuron, striatum, G5_1000701502-OGC22-sal_1_G8


title: single neuron, striatum, F7_1000701002-OGC14-sal_1_H10


title: single neuron, striatum, A10_1000701702-OGC17-sal_1_G12


title: single neuron, striatum, D2_1000701806-OGC18-sal_1_K1


title: single neuron, striatum, E9_1000701806-OGC18-sal_1_K2


title: single neuron, striatum, E4_1000701002-OGC14-sal_1_J9


title: single neuron, striatum, F6_1000701805-OGC18-sal_1_M21


title: single neuron, striatum, F4_1000701502-OGC22-sal_1_N7


title: single neuron, striatum, G6_1000701002-OGC14-sal_1_P10


title: single neuron, striatum, D3_1000701002-OGC14-sal_1_O8


title: single neuron, striatum, A5_1000701805-OGC18-sal_1_A18


title: single neuron, striatum, B9_1000701002-OGC14-sal_1_P7


title: single neuron, striatum, C7_1000701502-OGC22-sal_1_D6


title: single neuron, striatum, B6_1000701806-OGC18-sal_1_I24


title: single neuron, striatum, E3_1000701805-OGC18-sal_1_N20


title: single neuron, striatum, D1_1000701002-OGC14-sal_1_M8


title: single neuron, striatum, A11_1000701002-OGC14-sal_1_G7


title: single neuron, striatum, A12_1000701806-OGC18-sal_1_C24


title: single neuron, striatum, A3_1000701702-OGC17-sal_1_P11


title: single neuron, striatum, D4_1000701002-OGC14-sal_1_P8


title: single neuron, striatum, D2_1000701702-OGC17-sal_1_C14


title: single neuron, striatum, D5_1000701702-OGC17-sal_1_F14


title: single neuron, striatum, A10_1000701805-OGC18-sal_1_F18


title: single neuron, striatum, H9_1000701502-OGC22-sal_1_C9


title: single neuron, striatum, H8_1000701002-OGC14-sal_1_K11


title: single neuron, striatum, A5_1000701806-OGC18-sal_1_L23


title: single neuron, striatum, D6_1000701502-OGC22-sal_1_N6


title: single neuron, striatum, E7_1000701806-OGC18-sal_1_J2


title: single neuron, striatum, G6_1000701702-OGC17-sal_1_K16


title: single neuron, striatum, E11_1000701002-OGC14-sal_1_A10


title: single neuron, striatum, B12_1000701702-OGC17-sal_1_E13


title: single neuron, striatum, A7_1000701806-OGC18-sal_1_N23


title: single neuron, striatum, F5_1000701805-OGC18-sal_1_L21


title: single neuron, striatum, A9_1000701805-OGC18-sal_1_E18


title: single neuron, striatum, D11_1000701805-OGC18-sal_1_J20


title: single neuron, striatum, E4_1000701502-OGC22-sal_1_G7


title: single neuron, striatum, C6_1000701002-OGC14-sal_1_G8


title: single neuron, striatum, B11_1000701002-OGC14-sal_1_B8


title: single neuron, striatum, B1_1000701002-OGC14-sal_1_I7


title: single neuron, striatum, C3_1000701002-OGC14-sal_1_E8


title: single neuron, striatum, F6_1000701806-OGC18-sal_1_B3


title: single neuron, striatum, C8_1000701805-OGC18-sal_1_L19


title: single neuron, striatum, H4_1000701805-OGC18-sal_1_B23


title: single neuron, striatum, B2_1000701806-OGC18-sal_1_E24


title: single neuron, striatum, B8_1000701805-OGC18-sal_1_P18


title: single neuron, striatum, G10_1000701805-OGC18-sal_1_L22


title: single neuron, striatum, F10_1000701806-OGC18-sal_1_F3


title: single neuron, striatum, C7_1000701805-OGC18-sal_1_K19


title: single neuron, striatum, A5_1000701502-OGC22-sal_1_P4


title: single neuron, striatum, G1_1000701502-OGC22-sal_1_D8


title: single neuron, striatum, G4_1000701002-OGC14-sal_1_N10


title: single neuron, striatum, A4_1000701502-OGC22-sal_1_O4


title: single neuron, striatum, E6_1000701702-OGC17-sal_1_C15


title: single neuron, striatum, G3_1000701805-OGC18-sal_1_E22


title: single neuron, striatum, G8_1000701702-OGC17-sal_1_M16


title: single neuron, striatum, E3_1000701702-OGC17-sal_1_P14


title: single neuron, striatum, B1_1000701702-OGC17-sal_1_J12


title: single neuron, striatum, A11_1000701805-OGC18-sal_1_G18


title: single neuron, striatum, C1_1000701805-OGC18-sal_1_E19


title: single neuron, striatum, B6_1000701805-OGC18-sal_1_N18


title: single neuron, striatum, E9_1000701002-OGC14-sal_1_O9


title: single neuron, striatum, H7_1000701806-OGC18-sal_1_H4


title: single neuron, striatum, A10_1000701002-OGC14-sal_1_F7


title: single neuron, striatum, C5_1000701002-OGC14-sal_1_F8


title: single neuron, striatum, G9_1000701702-OGC17-sal_1_N16


title: single neuron, striatum, A7_1000701702-OGC17-sal_1_D12


title: single neuron, striatum, A11_1000701806-OGC18-sal_1_B24


title: single neuron, striatum, G4_1000701805-OGC18-sal_1_F22


title: single neuron, striatum, C2_1000701502-OGC22-sal_1_O5


title: single neuron, striatum, F2_1000701002-OGC14-sal_1_D10


title: single neuron, striatum, E3_1000701806-OGC18-sal_1_G2


title: single neuron, striatum, B5_1000701806-OGC18-sal_1_H24


title: single neuron, striatum, D3_1000701702-OGC17-sal_1_D14


title: single neuron, striatum, A4_1000701002-OGC14-sal_1_A7


title: single neuron, striatum, G6_1000701502-OGC22-sal_1_H8


title: single neuron, striatum, E7_1000701502-OGC22-sal_1_I7


title: single neuron, striatum, G08_10007018-NA_1_K08__N420Barcode_710-508


title: single neuron, striatum, F10_10007018-NA_1_A08__N420Barcode_710-502


title: single neuron, striatum, B07_10007018-NA_1_N04__N420Barcode_704-521


title: single neuron, striatum, D11_10007018-NA_1_J06__N420Barcode_706-518


title: single neuron, striatum, E08_10007018-NA_1_C07__N420Barcode_707-503


title: single neuron, striatum, D09_10007018-NA_1_H06__N420Barcode_706-517


title: single neuron, striatum, C01_10007018-NA_1_D05__N420Barcode_705-515


title: single neuron, striatum, H09_10007017-OGC17-sal_1_J03__N420Barcode_703-518


title: single neuron, striatum, D04_10007018-NA_1_C06__N420Barcode_706-503


title: single neuron, striatum, B06_10007018-NA_1_M04__N420Barcode_704-510


title: single neuron, striatum, H04_10007017-OGC17-sal_1_E03__N420Barcode_703-505


title: single neuron, striatum, H05_10007017-OGC17-sal_1_F03__N420Barcode_703-516


title: single neuron, striatum, E06_10007018-NA_1_A07__N420Barcode_707-502


title: single neuron, striatum, H05_10007018-NA_1_B09__N420Barcode_711-513


title: single neuron, striatum, A06_10007018-NA_1_B04__N420Barcode_704-513


title: single neuron, striatum, D05_10007018-NA_1_D06__N420Barcode_706-515


title: single neuron, striatum, F02_10007018-NA_1_I07__N420Barcode_707-507


title: single neuron, striatum, C11_10007018-NA_1_N05__N420Barcode_705-521


title: single neuron, striatum, E11_10007017-OGC17-sal_1_H01__N420Barcode_701-517


title: single neuron, striatum, H06_10007018-NA_1_C09__N420Barcode_711-503


title: single neuron, striatum, G12_10007018-NA_1_O08__N420Barcode_710-511


title: single neuron, striatum, A07_10007018-NA_1_C04__N420Barcode_704-503


title: single neuron, striatum, E05_10007018-NA_1_P06__N420Barcode_706-522


title: single neuron, striatum, F11_10007018-NA_1_B08__N420Barcode_710-513


title: single neuron, striatum, G11_1000701702-OGC17-sal_1_P16


title: single neuron, striatum, A04_10007018-NA_1_P03__N420Barcode_703-522


title: single neuron, striatum, F12_10007017-OGC17-sal_1_E02__N420Barcode_702-505


title: single neuron, striatum, E12_10007018-NA_1_G07__N420Barcode_707-506


title: single neuron, striatum, G10_10007018-NA_1_M08__N420Barcode_710-510


title: single neuron, striatum, F09_10007018-NA_1_P07__N420Barcode_707-522


title: single neuron, striatum, G04_10007018-NA_1_G08__N420Barcode_710-506


title: single neuron, striatum, A05_10007018-NA_1_A04__N420Barcode_704-502


title: single neuron, striatum, A11_10007018-NA_1_F04__N420Barcode_704-516


title: single neuron, striatum, F03_10007017-OGC17-sal_1_L01__N420Barcode_701-520


title: single neuron, striatum, F03_10007018-NA_1_J07__N420Barcode_707-518


title: single neuron, striatum, G01_10007017-OGC17-sal_1_F02__N420Barcode_702-516


title: single neuron, striatum, F08_10007017-OGC17-sal_1_A02__N420Barcode_702-502


title: single neuron, striatum, B03_10007018-NA_1_J04__N420Barcode_704-518


title: single neuron, striatum, D01_10007018-NA_1_P05__N420Barcode_705-522


title: single neuron, striatum, H03_10007017-OGC17-sal_1_D03__N420Barcode_703-515


title: single neuron, striatum, C02_10007018-NA_1_E05__N420Barcode_705-505


title: single neuron, striatum, A12_10007018-NA_1_G04__N420Barcode_704-506


title: single neuron, striatum, A10_10007018-NA_1_E04__N420Barcode_704-505


title: single neuron, striatum, F01_10007018-NA_1_H07__N420Barcode_707-517


title: single neuron, striatum, H11_10007017-OGC17-sal_1_L03__N420Barcode_703-520


title: single neuron, striatum, F04_10007018-NA_1_K07__N420Barcode_707-508


title: single neuron, striatum, G09_10007017-OGC17-sal_1_N02__N420Barcode_702-521


title: single neuron, striatum, C06_10007018-NA_1_I05__N420Barcode_705-507


title: single neuron, striatum, F05_10007017-OGC17-sal_1_N01__N420Barcode_701-521


title: single neuron, striatum, D10_10007018-NA_1_I06__N420Barcode_706-507


title: single neuron, striatum, D12_10007018-NA_1_K06__N420Barcode_706-508


title: single neuron, striatum, A03_10007018-NA_1_O03__N420Barcode_703-511


title: single neuron, striatum, F05_10007018-NA_1_L07__N420Barcode_707-520


title: single neuron, striatum, F01_10007017-OGC17-sal_1_J01__N420Barcode_701-518


title: single neuron, striatum, E09_10007017-OGC17-sal_1_F01__N420Barcode_701-516


title: single neuron, striatum, F06_10007018-NA_1_M07__N420Barcode_707-510


title: single neuron, striatum, C08_10007018-NA_1_K05__N420Barcode_705-508


title: single neuron, striatum, C04_10007018-NA_1_G05__N420Barcode_705-506


title: single neuron, striatum, H07_10007018-NA_1_D09__N420Barcode_711-515


title: single neuron, striatum, F10_10007017-OGC17-sal_1_C02__N420Barcode_702-503


title: single neuron, striatum, E06_10007017-OGC17-sal_1_C01__N420Barcode_701-503


title: single neuron, striatum, E04_10007018-NA_1_O06__N420Barcode_706-511


title: single neuron, striatum, C03_10007018-NA_1_F05__N420Barcode_705-516


title: single neuron, striatum, B08_10007018-NA_1_O04__N420Barcode_704-511


title: single neuron, striatum, G11_10007017-OGC17-sal_1_P02__N420Barcode_702-522


title: single neuron, striatum, H02_10007017-OGC17-sal_1_C03__N420Barcode_703-503


title: single neuron, striatum, G05_10007017-OGC17-sal_1_J02__N420Barcode_702-518


title: single neuron, striatum, G12_10007017-OGC17-sal_1_A03__N420Barcode_703-502


title: single neuron, striatum, G08_10007017-OGC17-sal_1_M02__N420Barcode_702-510


title: single neuron, striatum, G03_10007018-NA_1_F08__N420Barcode_710-516


title: single neuron, striatum, F02_10007017-OGC17-sal_1_K01__N420Barcode_701-508


title: single neuron, striatum, C10_10007018-NA_1_M05__N420Barcode_705-510


title: single neuron, striatum, B11_10007018-NA_1_B05__N420Barcode_705-513


title: single neuron, striatum, F12_10007018-NA_1_C08__N420Barcode_710-503


title: single neuron, striatum, H10_10007018-NA_1_G09__N420Barcode_711-506


title: single neuron, striatum, E08_10007017-OGC17-sal_1_E01__N420Barcode_701-505


title: single neuron, striatum, G06_10007018-NA_1_I08__N420Barcode_710-507


title: single neuron, striatum, C12_10007018-NA_1_O05__N420Barcode_705-511


title: single neuron, striatum, G02_10007017-OGC17-sal_1_G02__N420Barcode_702-506


title: single neuron, striatum, D07_10007018-NA_1_F06__N420Barcode_706-516


title: single neuron, striatum, G06_10007017-OGC17-sal_1_K02__N420Barcode_702-508


title: single neuron, striatum, B02_10007018-NA_1_I04__N420Barcode_704-507


title: single neuron, striatum, G09_10007018-NA_1_L08__N420Barcode_710-520


title: single neuron, striatum, F06_10007017-OGC17-sal_1_O01__N420Barcode_701-511


title: single neuron, striatum, G02_10007018-NA_1_E08__N420Barcode_710-505


title: single neuron, striatum, A09_10007018-NA_1_D04__N420Barcode_704-515


title: single neuron, striatum, G05_10007018-NA_1_H08__N420Barcode_710-517


title: single neuron, striatum, B04_10007018-NA_1_K04__N420Barcode_704-508


title: single neuron, striatum, F09_10007017-OGC17-sal_1_B02__N420Barcode_702-513


title: single neuron, striatum, E12_10007017-OGC17-sal_1_I01__N420Barcode_701-507


title: single neuron, striatum, B01_10007018-NA_1_H04__N420Barcode_704-517


title: single neuron, striatum, A01_10007018-NA_1_M03__N420Barcode_703-510


title: single neuron, striatum, C09_10007018-NA_1_L05__N420Barcode_705-520


title: single neuron, striatum, H08_10007018-NA_1_E09__N420Barcode_711-505


title: single neuron, striatum, B12_10007018-NA_1_C05__N420Barcode_705-503


title: single neuron, striatum, E10_10007018-NA_1_E07__N420Barcode_707-505


title: single neuron, striatum, D08_10007018-NA_1_G06__N420Barcode_706-506


title: single neuron, striatum, E03_10007018-NA_1_N06__N420Barcode_706-521


title: single neuron, striatum, H08_10007017-OGC17-sal_1_I03__N420Barcode_703-507


title: single neuron, striatum, C05_10007018-NA_1_H05__N420Barcode_705-517


title: single neuron, striatum, E05_10007017-OGC17-sal_1_B01__N420Barcode_701-513


title: single neuron, striatum, F08_10007018-NA_1_O07__N420Barcode_707-511


title: single neuron, striatum, H09_10007018-NA_1_F09__N420Barcode_711-516


title: single neuron, striatum, E07_10007018-NA_1_B07__N420Barcode_707-513


title: single neuron, striatum, E11_10007018-NA_1_F07__N420Barcode_707-516


title: single neuron, striatum, D06_10007018-NA_1_E06__N420Barcode_706-505


title: single neuron, striatum, G07_10007018-NA_1_J08__N420Barcode_710-518


title: single neuron, striatum, F04_10007017-OGC17-sal_1_M01__N420Barcode_701-510


title: single neuron, striatum, H11_10007018-NA_1_H09__N420Barcode_711-517


title: single neuron, striatum, H03_10007018-NA_1_A09__N420Barcode_711-502


title: single neuron, striatum, H02_10007018-NA_1_P08__N420Barcode_710-522


title: single neuron, striatum, B10_10007018-NA_1_A05__N420Barcode_705-502


title: single neuron, striatum, H01_10007017-OGC17-sal_1_B03__N420Barcode_703-513


title: single neuron, striatum, H10_10007017-OGC17-sal_1_K03__N420Barcode_703-508


title: single neuron, striatum, G01_10007018-NA_1_D08__N420Barcode_710-515


title: single neuron, striatum, D02_10007018-NA_1_A06__N420Barcode_706-502


title: single neuron, striatum, E09_10007018-NA_1_D07__N420Barcode_707-515


title: single neuron, striatum, E01_10007018-NA_1_L06__N420Barcode_706-520


title: single neuron, striatum, F07_10007018-NA_1_N07__N420Barcode_707-521


title: single neuron, striatum, C07_10007018-NA_1_J05__N420Barcode_705-518


title: single neuron, striatum, E07_10007017-OGC17-sal_1_D01__N420Barcode_701-515


title: single neuron, striatum, D03_10007018-NA_1_B06__N420Barcode_706-513


title: single neuron, striatum, A02_10007018-NA_1_N03__N420Barcode_703-521


title: single neuron, striatum, E10_10007013-OGC20-sal_1_K09__N419Barcode_711-508


title: single neuron, striatum, E04_10007015-OGC22-sal_1_M19__N419Barcode_723-510


title: single neuron, striatum, B09_10007018-NA_1_P04__N420Barcode_704-522


title: single neuron, striatum, G07_10007017-OGC17-sal_1_L02__N420Barcode_702-520


title: single neuron, striatum, D05_10007013-OGC20-sal_1_J08__N419Barcode_710-518


title: single neuron, striatum, D09_10007015-OGC22-sal_1_G19__N419Barcode_723-506


title: single neuron, striatum, C01_10007017-OGC17-sal_1_G23__N419Barcode_728-506


title: single neuron, striatum, D09_10007017-OGC17-sal_1_J24__N419Barcode_729-518


title: single neuron, striatum, B01_10007013-OGC20-sal_1_N06__N419Barcode_706-521


title: single neuron, striatum, C11_10007015-OGC22-sal_1_N18__N419Barcode_722-521


title: single neuron, striatum, G06_10007015-OGC22-sal_1_B21__N419Barcode_726-513


title: single neuron, striatum, H11_10007015-OGC22-sal_1_N21__N419Barcode_726-521


title: single neuron, striatum, D11_10007017-OGC17-sal_1_L24__N419Barcode_729-520


title: single neuron, striatum, B06_10007015-OGC22-sal_1_O17__N419Barcode_721-511


title: single neuron, striatum, D04_10007015-OGC22-sal_1_C19__N419Barcode_723-503


title: single neuron, striatum, G12_10007013-OGC20-sal_1_D11__N419Barcode_714-515


title: single neuron, striatum, A05_10007017-OGC17-sal_1_C22__N419Barcode_727-503


title: single neuron, striatum, C07_10007017-OGC17-sal_1_M23__N419Barcode_728-510


title: single neuron, striatum, A12_10007013-OGC20-sal_1_M06__N419Barcode_706-510


title: single neuron, striatum, C12_10007013-OGC20-sal_1_E08__N419Barcode_710-505


title: single neuron, striatum, F03_10007013-OGC20-sal_1_P09__N419Barcode_711-522


title: single neuron, striatum, E01_10007017-OGC17-sal_1_N24__N419Barcode_729-521


title: single neuron, striatum, F08_10007013-OGC20-sal_1_E10__N419Barcode_712-505


title: single neuron, striatum, H02_10007015-OGC22-sal_1_I21__N419Barcode_726-507


title: single neuron, striatum, F06_10007013-OGC20-sal_1_C10__N419Barcode_712-503


title: single neuron, striatum, D10_10007013-OGC20-sal_1_O08__N419Barcode_710-511


title: single neuron, striatum, F02_10007015-OGC22-sal_1_E20__N419Barcode_724-505


title: single neuron, striatum, E07_10007015-OGC22-sal_1_O19__N419Barcode_723-511


title: single neuron, striatum, E12_10007015-OGC22-sal_1_C20__N419Barcode_724-503


title: single neuron, striatum, D12_10007013-OGC20-sal_1_A09__N419Barcode_711-502


title: single neuron, striatum, H09_10007013-OGC20-sal_1_M11__N419Barcode_714-510


title: single neuron, striatum, A06_10007013-OGC20-sal_1_G06__N419Barcode_706-506


title: single neuron, striatum, A05_10007013-OGC20-sal_1_F06__N419Barcode_706-516


title: single neuron, striatum, A11_10007017-OGC17-sal_1_I22__N419Barcode_727-507


title: single neuron, striatum, C02_10007015-OGC22-sal_1_E18__N419Barcode_722-505


title: single neuron, striatum, B03_10007017-OGC17-sal_1_M22__N419Barcode_727-510


title: single neuron, striatum, A07_10007013-OGC20-sal_1_H06__N419Barcode_706-517


title: single neuron, striatum, G05_10007015-OGC22-sal_1_A21__N419Barcode_726-502


title: single neuron, striatum, B07_10007017-OGC17-sal_1_A23__N419Barcode_728-502


title: single neuron, striatum, G08_10007015-OGC22-sal_1_D21__N419Barcode_726-515


title: single neuron, striatum, G04_10007015-OGC22-sal_1_P20__N419Barcode_724-522


title: single neuron, striatum, D06_10007013-OGC20-sal_1_K08__N419Barcode_710-508


title: single neuron, striatum, A03_10007017-OGC17-sal_1_A22__N419Barcode_727-502


title: single neuron, striatum, B08_10007015-OGC22-sal_1_A18__N419Barcode_722-502


title: single neuron, striatum, D06_10007017-OGC17-sal_1_G24__N419Barcode_729-506


title: single neuron, striatum, G09_10007013-OGC20-sal_1_A11__N419Barcode_714-502


title: single neuron, striatum, C06_10007015-OGC22-sal_1_I18__N419Barcode_722-507


title: single neuron, striatum, C08_10007013-OGC20-sal_1_A08__N419Barcode_710-502


title: single neuron, striatum, C05_10007015-OGC22-sal_1_H18__N419Barcode_722-517


title: single neuron, striatum, E06_10007015-OGC22-sal_1_N19__N419Barcode_723-521


title: single neuron, striatum, A04_10007017-OGC17-sal_1_B22__N419Barcode_727-513


title: single neuron, striatum, E11_10007013-OGC20-sal_1_L09__N419Barcode_711-520


title: single neuron, striatum, E12_10007013-OGC20-sal_1_M09__N419Barcode_711-510


title: single neuron, striatum, G07_10007013-OGC20-sal_1_O10__N419Barcode_712-511


title: single neuron, striatum, F02_10007013-OGC20-sal_1_O09__N419Barcode_711-511


title: single neuron, striatum, C12_10007015-OGC22-sal_1_O18__N419Barcode_722-511