title: cy80-CD45-neg-C01-S889-comb

description: single cell RNA-seq


title: cy79-p5-CD45-pos-PD1-pos-F11-S647-comb

status: Public on Apr 05 2016

type: SRA

city: Rehovot

first: Itay

last: Tirosh

address: Herzl 234

country: Israel

institute: WEIZMANN INSTITUTE OF SCIENCE

description: single cell RNA-seq

library_source: transcriptomic

data_processing:
Following sequencing on the NextSeq, BAM files were converted to merged, demultiplexed FASTQs. Paired-end reads were then mapped to the UCSC hg19 human transcriptome using Bowtie with parameters "-q --phred33-quals -n 1 -e 99999999 -l 25 -I 1 -X 2000 -a -m 15 -S -p 6", which allows alignment of sequences with single base changes such as due to point mutations. Expression levels of genes were quantified as Ei,j=log2(TPMi,j/10+1), where TPMi,j refers to transcript-per-million (TPM) for gene i in sample j, as calculated by RSEM v1.2.3 in paired-end mode. TPM values were divided by 10 since we estimate the complexity of our single cell libraries to be on the order of 100,000 transcripts and would like to avoid counting each transcript ~10 times, as would be the case with TPM, which may inflate the difference between the expression level of a gene in cells in which the gene is detected and those in which it is not detected. For each cell, we quantified the number of genes for which at least one read was mapped, and the average expression level of a curated list of housekeeping genes. We then excluded all cells with either fewer than 1,700 detected genes or an average housekeeping expression (E, as defined above) below 3. Only genes detected with E>2 in at least 5 cells were retained in the processed data file.
Genome_build: hg19
Supplementary_files_format_and_content: Tab-delimited text file containing the normalized expression levels (E) for all genes with E>2 in at least 5 cells, across 3,249 cells that passed QC. the tumor-of-origin is indicated in the name of each cell (CY##). The first row denotes the classification (based on inferred CNVs) to malignant (2), non-malignant (1) or unresolved (0) cells. The second row denotes the inferred cell types for non-malignant cells, including T-cells (1), B-cells (2), Macrophages (3), Endothelial cells (4) and CAFs (5). All other rows correspond to genes.

molecule: polyA RNA

organism: Homo sapiens

source_name: melanoma tumor

growth_protocol: no culturing (freshly-resected tumor samples)

extract_protocol:
RNA and DNA were isolated using the Qiagen minikit following the manufacturers recommendations.  Whole Transcriptome amplification (WTA) was performed with a modified SMART-Seq2 protocol, with Maxima Reverse Transcriptase (Life Technologies) used in place of superscript II.
WTA products were cleaned with Agencourt XP DNA beads and 70% ethanol (Beckman Coulter) and Illumina sequencing libraries were prepared using Nextera XT (Illumina), as previously described (15). The 96 samples of a multiwall plate were pooled together, and cleaned with two 0.8x DNA SPRIs (Beckman Coulter). Library quality was assessed with a high sensitivity DNA chip (Agilent) and quantified with a high sensitivity dsDNA Quant Kit (Life Technologies). Samples were sequenced on an Illumina NextSeq 500 instrument using 30bp paired-end reads.

treatment_protocol: Resected tumors were transported in DMEM (ThermoFisher Scientific) on ice immediately after surgical procurement. Tumors were rinsed with PBS (Life Technologies). A small fragment was stored in RNA-protect (Qiagen) for bulk RNA and DNA isolation. Using scalpels, the remainder of the tumor was minced into tiny cubes <1 mm3 and transferred into a 50 ml conical tube (BD Falcon) containing 10 ml pre-warmed M199-media (ThermoFisher Scientific), 2 mg/ml collagenase P (Roche) and 10U/μl DNase I (Roche). Tumor pieces were digested in this digestion media for 10 minutes at 37°C, then vortexed for 10 seconds and pipetted up and down for 1 minute using pipettes of descending sizes (25 ml, 10 ml and 5 ml). If needed, this was repeated twice more until a single-cell suspension was obtained. This suspension was then filtered using a 70μm mesh (ThermoFisher Scientific) and residual cell clumps were discarded. The suspension was supplemented with 30 ml PBS (Life Technologies) with 2% fetal calf serum (FCS) (Gemini Bioproducts) and immediately placed on ice. After centrifuging at 580g at 4°C for 6 minutes, the supernatant was discarded and the cell pellet was re-suspended in PBS with FCS and placed on ice prior to staining for FACS. Single-cell suspensions were stained with CD45-FITC (VWR), Calcein-AM (Life Technologies) and CD90-PE (BioLegend) per manufacturer recommendations. First, doublets were excluded based on forward and sideward scatter, then we gated on viable cells (Calceinhigh) and sorted single cells (CD45+ or CD45-CD90+ or CD45-CD90-) into 96-well plates chilled to 4°C, pre-prepared with 10μl TCL buffer (Qiagen) supplemented with 1% beta-mercaptoethanol (lysis buffer). Single-cell lysates were vortexed, spun down at 3700 rpm at 4°C for 2 minutes, immediately placed on dry ice and transferred for storage at -80°C.

tag: tumor-of-origin


title: cy80-CD45-neg-A12-S876-comb

description: single cell RNA-seq


title: cy82-CD45-pos-3-G06-S78-comb

description: single cell RNA-seq


title: cy79-p5-CD45-pos-PD1-pos-G09-S657-comb

description: single cell RNA-seq


title: cy81-FNA-CD45-neg-G07-S1039-comb

description: single cell RNA-seq


title: cy79-p4-CD45-neg-PDL1-neg-G05-S1133-comb

description: single cell RNA-seq


title: cy80-CD45-neg-E04-S916-comb

description: single cell RNA-seq


title: cy79-p5-CD45-neg-PDL1-pos-D02-S710-comb

description: single cell RNA-seq


title: cy79-p5-CD45-pos-PD1-pos-F07-S643-comb

description: single cell RNA-seq


title: cy81-Bulk-CD45-neg-F02-S158-comb

description: single cell RNA-seq


title: cy79-p4-CD45-neg-PDL1-neg-B05-S1073-comb

description: single cell RNA-seq


title: cy79-p4-CD45-pos-PD1-pos-B01-S301-comb

description: single cell RNA-seq


title: cy79-p4-CD45-neg-PDL1-neg-A04-S1060-comb

description: single cell RNA-seq


title: cy79-p4-CD45-neg-PDL1-neg-E03-S1107-comb

description: single cell RNA-seq


title: cy79-p5-CD45-pos-PD1-pos-H12-S672-comb

description: single cell RNA-seq


title: cy82-CD45-pos-1-D05-S521-comb

description: single cell RNA-seq


title: cy81-FNA-CD45-neg-C07-S991-comb

description: single cell RNA-seq


title: cy81-Bulk-CD45-neg-H11-S191-comb

description: single cell RNA-seq


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description: single cell RNA-seq


title: cy81-Bulk-CD45-neg-D05-S137-comb

description: single cell RNA-seq


title: cy79-p5-CD45-pos-PD1-pos-F01-S637-comb

description: single cell RNA-seq


title: cy79-p4-CD45-neg-PDL1-neg-C04-S1084-comb

description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


title: cy79-p4-CD45-neg-PDL1-neg-G11-S1139-comb

description: single cell RNA-seq


title: cy80-CD45-neg-B10-S886-comb

description: single cell RNA-seq


title: cy79-p4-CD45-neg-PDL1-pos-B04-S400-comb

description: single cell RNA-seq


title: cy79-p4-CD45-pos-PD1-pos-B12-S312-comb

description: single cell RNA-seq


title: cy80-CD45-neg-F07-S931-comb

description: single cell RNA-seq


title: cy80-CD45-neg-C11-S899-comb

description: single cell RNA-seq


title: cy79-p4-CD45-neg-PDL1-pos-A05-S389-comb

description: single cell RNA-seq


title: cy79-p5-CD45-neg-PDL1-pos-A08-S680-comb

description: single cell RNA-seq


title: cy79-p5-CD45-pos-PD1-pos-C12-S612-comb

description: single cell RNA-seq


title: cy79-p5-CD45-pos-PD1-pos-D11-S623-comb

description: single cell RNA-seq


title: cy79-p5-CD45-pos-PD1-pos-G06-S654-comb

description: single cell RNA-seq


title: cy80-CD45-neg-B12-S888-comb

description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


title: cy79-p5-CD45-neg-PDL1-pos-B11-S695-comb

description: single cell RNA-seq


title: cy79-p5-CD45-neg-PDL1-neg-B12-S792-comb

description: single cell RNA-seq


title: cy79-p5-CD45-pos-PD1-pos-H11-S671-comb

description: single cell RNA-seq


title: cy79-p4-CD45-pos-PD1-pos-D05-S329-comb

description: single cell RNA-seq


title: cy79-p4-CD45-neg-PDL1-pos-B09-S405-comb

description: single cell RNA-seq


title: cy79-p5-CD45-neg-PDL1-pos-D10-S718-comb

description: single cell RNA-seq


title: cy79-p5-CD45-pos-PD1-pos-G11-S659-comb

description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


title: cy79-p4-CD45-neg-PDL1-neg-H02-S1142-comb

description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


title: cy81-FNA-CD45-neg-H11-S1055-comb

description: single cell RNA-seq


title: cy79-p5-CD45-neg-PDL1-neg-D08-S812-comb

description: single cell RNA-seq


title: cy79-p4-CD45-neg-PDL1-neg-H07-S1147-comb

description: single cell RNA-seq


title: cy79-p4-CD45-pos-PD1-pos-H01-S373-comb

description: single cell RNA-seq


title: cy79-p4-CD45-pos-PD1-pos-C02-S314-comb

description: single cell RNA-seq


title: cy79-p5-CD45-neg-PDL1-pos-B08-S692-comb

description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


title: cy79-p5-CD45-pos-PD1-pos-B05-S593-comb

description: single cell RNA-seq


title: cy79-p4-CD45-pos-PD1-pos-H02-S374-comb

description: single cell RNA-seq


title: cy81-FNA-CD45-neg-E10-S1018-comb

description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


title: cy79-p4-CD45-neg-PDL1-pos-G10-S466-comb

description: single cell RNA-seq


title: cy79-p4-CD45-pos-PD1-pos-E03-S339-comb

description: single cell RNA-seq


title: cy79-p5-CD45-pos-PD1-pos-A11-S587-comb

description: single cell RNA-seq


title: cy79-p4-CD45-pos-PD1-pos-D09-S333-comb

description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


title: cy79-p5-CD45-neg-PDL1-pos-C05-S701-comb

description: single cell RNA-seq


title: cy79-p5-CD45-pos-PD1-pos-H04-S664-comb

description: single cell RNA-seq


title: cy79-p5-CD45-neg-PDL1-pos-E01-S721-comb

description: single cell RNA-seq


title: cy81-Bulk-CD45-neg-B04-S112-comb

description: single cell RNA-seq


title: cy79-p4-CD45-neg-PDL1-neg-G02-S1130-comb

description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


title: cy79-p5-CD45-neg-PDL1-neg-A08-S776-comb

description: single cell RNA-seq


title: cy79-p4-CD45-pos-PD1-pos-A07-S295-comb

description: single cell RNA-seq


title: cy79-p4-CD45-neg-PDL1-pos-G09-S465-comb

description: single cell RNA-seq


title: cy79-p5-CD45-neg-PDL1-neg-D02-S806-comb

description: single cell RNA-seq


title: cy79-p4-CD45-pos-PD1-pos-E08-S344-comb

description: single cell RNA-seq


title: cy79-p5-CD45-pos-PD1-pos-G07-S655-comb

description: single cell RNA-seq


title: cy79-p4-CD45-neg-PDL1-neg-D08-S1100-comb

description: single cell RNA-seq


title: cy79-p5-CD45-pos-PD1-pos-B03-S591-comb

description: single cell RNA-seq


title: cy79-p4-CD45-pos-PD1-pos-G04-S364-comb

description: single cell RNA-seq


title: cy79-p4-CD45-pos-PD1-pos-F09-S357-comb

description: single cell RNA-seq


title: cy79-p4-CD45-neg-PDL1-pos-B12-S408-comb

description: single cell RNA-seq


title: cy79-p4-CD45-pos-PD1-pos-A08-S296-comb

description: single cell RNA-seq


title: cy82-CD45-pos-3-H12-S96-comb

description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


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description: single cell RNA-seq


title: cy79-p4-CD45-neg-PDL1-pos-G04-S460-comb

description: single cell RNA-seq


title: cy79-p4-CD45-neg-PDL1-neg-G12-S1140-comb

description: single cell RNA-seq


title: cy80-CD45-neg-F08-S932-comb

description: single cell RNA-seq


title: cy81-Bulk-CD45-neg-G06-S174-comb

description: single cell RNA-seq


title: CY88_5_B08_S692_comb

description: single cell RNA-seq


title: CY88_3_A04_S580_comb

description: single cell RNA-seq


title: CY88_5_D12_S720_comb

description: single cell RNA-seq


title: cy79-p5-CD45-pos-PD1-pos-C05-S605-comb

description: single cell RNA-seq


title: CY88_5_G08_S752_comb

description: single cell RNA-seq


title: cy79-p5-CD45-pos-PD1-pos-D06-S618-comb

description: single cell RNA-seq


title: CY88_3_A05_S581_comb

description: single cell RNA-seq


title: cy79-p5-CD45-neg-PDL1-pos-A05-S677-comb

description: single cell RNA-seq


title: CY88_5_C11_S707_comb

description: single cell RNA-seq


title: CY88_3_F08_S644_comb

description: single cell RNA-seq


title: CY88_5_A07_S679_comb

description: single cell RNA-seq


title: CY88_5_A09_S681_comb

description: single cell RNA-seq


title: CY88_5_B02_S686_comb

description: single cell RNA-seq


title: CY88_3_F06_S642_comb

description: single cell RNA-seq


title: CY88_3_H04_S664_comb

description: single cell RNA-seq


title: CY88_3_G10_S658_comb

description: single cell RNA-seq


title: CY88_3_D11_S623_comb

description: single cell RNA-seq


title: CY88_3_F09_S645_comb

description: single cell RNA-seq


title: CY88_3_E08_S632_comb

description: single cell RNA-seq


title: CY88_5_A12_S684_comb

description: single cell RNA-seq


title: CY88_5_E11_S731_comb

description: single cell RNA-seq


title: CY88_3_F10_S646_comb

description: single cell RNA-seq


title: CY88_5_A06_S678_comb

description: single cell RNA-seq


title: CY88_5_A03_S675_comb

description: single cell RNA-seq


title: CY88_3_G01_S649_comb

description: single cell RNA-seq


title: CY88_5_F03_S735_comb

description: single cell RNA-seq


title: CY88_3_A11_S587_comb

description: single cell RNA-seq


title: CY88_5_H12_S768_comb

description: single cell RNA-seq


title: CY88_3_G05_S653_comb

description: single cell RNA-seq


title: CY88_3_E07_S631_comb

description: single cell RNA-seq


title: CY88_3_C06_S606_comb

description: single cell RNA-seq


title: CY88_3_A08_S584_comb

description: single cell RNA-seq


title: CY88_5_F06_S738_comb

description: single cell RNA-seq


title: CY88_5_H04_S760_comb

description: single cell RNA-seq


title: CY88_3_F11_S647_comb

description: single cell RNA-seq


title: CY88_5_H06_S762_comb

description: single cell RNA-seq


title: CY88_3_E02_S626_comb

description: single cell RNA-seq


title: CY88_3_A02_S578_comb

description: single cell RNA-seq


title: CY88_3_D05_S617_comb

description: single cell RNA-seq


title: CY88_5_G02_S746_comb

description: single cell RNA-seq


title: CY88_5_C01_S697_comb

description: single cell RNA-seq


title: CY88_3_G12_S660_comb

description: single cell RNA-seq


title: CY88_3_B09_S597_comb

description: single cell RNA-seq


title: CY88_5_A05_S677_comb

description: single cell RNA-seq


title: CY88_3_H08_S668_comb

description: single cell RNA-seq


title: CY88_5_C05_S701_comb

description: single cell RNA-seq


title: CY88_3_G03_S651_comb

description: single cell RNA-seq


title: CY88_5_E01_S721_comb

description: single cell RNA-seq


title: CY88_3_B08_S596_comb

description: single cell RNA-seq


title: CY88_5_C02_S698_comb

description: single cell RNA-seq


title: CY88_3_E10_S634_comb

description: single cell RNA-seq


title: CY88_5_C08_S704_comb

description: single cell RNA-seq


title: CY88_5_B03_S687_comb

description: single cell RNA-seq


title: CY88_3_E09_S633_comb

description: single cell RNA-seq


title: CY88_5_F12_S744_comb

description: single cell RNA-seq


title: CY88_5_B07_S691_comb

description: single cell RNA-seq


title: CY88_3_G07_S655_comb

description: single cell RNA-seq


title: CY88_5_F05_S737_comb

description: single cell RNA-seq


title: CY88_5_B01_S685_comb

description: single cell RNA-seq


title: CY88_3_D09_S621_comb

description: single cell RNA-seq


title: CY88_3_G11_S659_comb

description: single cell RNA-seq


title: CY88_5_F11_S743_comb

description: single cell RNA-seq


title: CY88_5_E02_S722_comb

description: single cell RNA-seq


title: CY88_3_D03_S615_comb

description: single cell RNA-seq


title: CY88_3_C12_S612_comb

description: single cell RNA-seq


title: CY88_3_A07_S583_comb

description: single cell RNA-seq


title: CY88_3_H12_S672_comb

description: single cell RNA-seq


title: CY88_3_A12_S588_comb

description: single cell RNA-seq


title: CY88_5_D10_S718_comb

description: single cell RNA-seq


title: CY88_3_C08_S608_comb

description: single cell RNA-seq


title: CY88_3_B12_S600_comb

description: single cell RNA-seq


title: CY88_5_F08_S740_comb

description: single cell RNA-seq


title: CY88_5_B12_S696_comb

description: single cell RNA-seq


title: CY88_5_D08_S716_comb

description: single cell RNA-seq


title: CY88_3_A10_S586_comb

description: single cell RNA-seq


title: CY88_5_H05_S761_comb

description: single cell RNA-seq


title: CY88_3_B04_S592_comb

description: single cell RNA-seq


title: CY88_5_E04_S724_comb

description: single cell RNA-seq


title: CY88_5_C09_S705_comb

description: single cell RNA-seq


title: CY88_3_G09_S657_comb

description: single cell RNA-seq


title: CY88_5_F07_S739_comb

description: single cell RNA-seq


title: CY88_3_D07_S619_comb

description: single cell RNA-seq


title: CY88_3_C10_S610_comb

description: single cell RNA-seq


title: CY88_3_H11_S671_comb

description: single cell RNA-seq


title: CY88_3_F05_S641_comb

description: single cell RNA-seq


title: CY88_5_D11_S719_comb

description: single cell RNA-seq


title: CY88_3_D06_S618_comb

description: single cell RNA-seq


title: CY88_5_C12_S708_comb

description: single cell RNA-seq


title: CY88_3_C01_S601_comb

description: single cell RNA-seq


title: CY88_3_F01_S637_comb

description: single cell RNA-seq


title: CY88_5_B06_S690_comb

description: single cell RNA-seq


title: CY88_5_H03_S759_comb

description: single cell RNA-seq


title: CY88_5_B10_S694_comb

description: single cell RNA-seq


title: CY88_3_D02_S614_comb

description: single cell RNA-seq


title: CY88_3_H10_S670_comb

description: single cell RNA-seq


title: CY88_3_B02_S590_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_C03_S987_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_A11_S11_comb

description: single cell RNA-seq


title: cy80_CD_90_pos_H08_S956_comb

description: single cell RNA-seq


title: CY88_5_H08_S764_comb

description: single cell RNA-seq


title: cy80_CD_90_pos_C07_S895_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_G10_S82_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_B07_S979_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_B07_S19_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_B08_S308_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_E04_S340_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_C06_S30_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_B06_S978_comb

description: single cell RNA-seq


title: CY88_5_G04_S748_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_C04_S316_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_H05_S473_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_G06_S366_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_B04_S304_comb

description: single cell RNA-seq


title: cy80_cd_90_neg_H04_S184_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_A04_S964_comb

description: single cell RNA-seq


title: cy60_1_cd_45_neg_1_B11_S215_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_G12_S1044_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_C01_S313_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_F07_S1027_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_E08_S1016_comb

description: single cell RNA-seq


title: cy80_CD_90_pos_A02_S866_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_H08_S380_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_G01_S457_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_3_H12_S672_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_F10_S358_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_H05_S1049_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_B08_S20_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_F10_S1030_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_G12_S372_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_D10_S1006_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_B01_S397_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_H07_S379_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_F02_S1022_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_G03_S75_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_A01_S289_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_3_F02_S638_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_F03_S1023_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_E11_S1019_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_D02_S38_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_C05_S413_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_H01_S85_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_G05_S365_comb

description: single cell RNA-seq


title: cy80_CD_90_pos_C02_S890_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_B03_S15_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_B10_S310_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_H05_S377_comb

description: single cell RNA-seq


title: cy60_1_cd_45_neg_3_H01_S565_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_C04_S28_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_G04_S1036_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_D02_S422_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_3_B03_S591_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_D06_S330_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_B03_S303_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_3_H08_S668_comb

description: single cell RNA-seq


title: cy60_1_cd_45_neg_1_D07_S235_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_G11_S467_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_F11_S359_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_3_B11_S599_comb

description: single cell RNA-seq


title: cy60_1_cd_45_neg_3_G10_S562_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_E01_S49_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_G11_S371_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_3_F11_S647_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_A08_S296_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_E07_S343_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_C02_S410_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_E03_S1011_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_B12_S984_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_E06_S342_comb

description: single cell RNA-seq


title: cy60_1_cd_45_neg_3_H06_S570_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_C08_S320_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_H09_S93_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_D11_S335_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_H07_S1051_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_D12_S1008_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_B09_S21_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_H10_S1054_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_G07_S1039_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_F02_S350_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_H12_S480_comb

description: single cell RNA-seq


title: cy80_cd_90_neg_H12_S192_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_C11_S323_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_G08_S80_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_G10_S370_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_D05_S1001_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_H02_S374_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_F06_S354_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_H12_S96_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_B02_S302_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_A08_S968_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_E02_S1010_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_A04_S4_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_B02_S14_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_E09_S441_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_D09_S45_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_A09_S9_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_G02_S1034_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_3_D11_S623_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_F05_S449_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_3_F05_S641_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_D02_S326_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_D01_S421_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_3_F04_S640_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_E03_S435_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_3_C07_S607_comb

description: single cell RNA-seq


title: cy80_CD_90_pos_E05_S917_comb

description: single cell RNA-seq


title: cy60_1_cd_45_neg_1_C08_S224_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_B11_S983_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_H07_S91_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_G09_S81_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_A01_S1_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_G03_S363_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_C07_S31_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_A07_S295_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_G11_S83_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_F04_S448_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_C08_S32_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_B02_S974_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_F05_S1025_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_F02_S446_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_B04_S976_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_B03_S975_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_H02_S86_comb

description: single cell RNA-seq


title: cy60_1_cd_45_neg_1_D12_S240_comb

description: single cell RNA-seq


title: cy80_cd_90_neg_G12_S180_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_H11_S1055_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_C09_S993_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_D04_S328_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_G08_S1040_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_F03_S447_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_A10_S298_comb

description: single cell RNA-seq


title: cy80_CD_90_pos_B06_S882_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_B09_S405_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_F01_S349_comb

description: single cell RNA-seq


title: cy80_CD_90_pos_D03_S903_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_E11_S443_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_3_D09_S621_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_G08_S368_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_E09_S345_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_H01_S373_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_E04_S52_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_H08_S92_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_B06_S18_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_C04_S988_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_D07_S1003_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_F08_S1028_comb

description: single cell RNA-seq


title: cy80_CD_90_pos_E03_S915_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_D11_S47_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_H06_S378_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_H02_S1046_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_G07_S367_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_D09_S333_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_C11_S419_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_B12_S24_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_A06_S294_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_B05_S305_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_3_D03_S615_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_G05_S1037_comb

description: single cell RNA-seq


title: cy80_cd_90_neg_C03_S123_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_C01_S25_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_G04_S76_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_B07_S307_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_D08_S44_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_E07_S439_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_F09_S453_comb

description: single cell RNA-seq


title: cy80_CD_90_pos_G06_S942_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_3_B09_S597_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_H03_S87_comb

description: single cell RNA-seq


title: cy60_1_cd_45_neg_1_D11_S239_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_3_C09_S609_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_E10_S1018_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_E03_S51_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_C05_S989_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_C11_S35_comb

description: single cell RNA-seq


title: cy60_1_cd_45_neg_3_F09_S549_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_A03_S3_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_H03_S1047_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_E11_S347_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_3_F09_S645_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_H08_S1052_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_H06_S90_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_G07_S79_comb

description: single cell RNA-seq


title: cy80_CD_90_pos_B02_S878_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_E07_S1015_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_F07_S355_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_C09_S33_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_F06_S450_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_D07_S43_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_H01_S1045_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_D06_S42_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_B05_S977_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_E02_S338_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_D03_S327_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_E05_S341_comb

description: single cell RNA-seq


title: cy80_CD_90_pos_F07_S931_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_E08_S56_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_A10_S970_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_E08_S344_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_H11_S479_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_F08_S356_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_A12_S972_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_B01_S973_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_H06_S1050_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_D05_S329_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_B11_S407_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_D10_S46_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_C03_S315_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_H12_S384_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_F03_S351_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_D02_S998_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_C07_S319_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_H10_S382_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_E07_S55_comb

description: single cell RNA-seq


title: cy80_CD_90_pos_F09_S933_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_H07_S475_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_E06_S438_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_H04_S376_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_F07_S451_comb

description: single cell RNA-seq


title: cy80_CD_90_pos_C05_S893_comb

description: single cell RNA-seq


title: cy80_CD_90_pos_F03_S927_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_G02_S362_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_F05_S353_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_B06_S306_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_A12_S12_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_C10_S322_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_H06_S474_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_B11_S23_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_E06_S54_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_C12_S996_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_E02_S50_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_C10_S34_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_G04_S364_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_H10_S478_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_E01_S433_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_A05_S965_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_G05_S461_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_D06_S1002_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_E05_S437_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_B07_S403_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_A02_S290_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_D01_S325_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_G02_S74_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_D12_S48_comb

description: single cell RNA-seq


title: cy80_CD_90_pos_B04_S880_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_C09_S417_comb

description: single cell RNA-seq


title: cy60_1_cd_45_neg_3_C10_S514_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_E04_S1012_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_B12_S312_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_F04_S1024_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_B10_S982_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_D09_S429_comb

description: single cell RNA-seq


title: cy80_CD_90_pos_C04_S892_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_A10_S10_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_E09_S57_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_G02_S458_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_C07_S415_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_C05_S317_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_B11_S311_comb

description: single cell RNA-seq


title: cy80_CD_90_pos_D10_S910_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_3_B07_S595_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_3_B05_S593_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_A11_S299_comb

description: single cell RNA-seq


title: cy60_1_cd_45_neg_3_F06_S546_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_H12_S1056_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_G07_S463_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_G01_S361_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_E10_S58_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_H09_S1053_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_C03_S411_comb

description: single cell RNA-seq


title: cy80_CD_90_pos_E09_S921_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_A09_S297_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_B01_S301_comb

description: single cell RNA-seq


title: cy60_1_cd_45_neg_3_H07_S571_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_A03_S291_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_C06_S990_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_D01_S37_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_D11_S431_comb

description: single cell RNA-seq


title: cy80_CD_90_pos_E04_S916_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_3_F07_S643_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_C01_S409_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_F09_S1029_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_C03_S27_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_3_F01_S637_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_C01_S985_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_E12_S348_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_C11_S995_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_E01_S337_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_F11_S455_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_3_C01_S601_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_C05_S29_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_A09_S969_comb

description: single cell RNA-seq


title: cy80_CD_90_pos_C11_S899_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_E05_S1013_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_C12_S324_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_D01_S997_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_C07_S991_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_A04_S292_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_D03_S39_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_G03_S459_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_H11_S383_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_A05_S293_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_C10_S994_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_G12_S84_comb

description: single cell RNA-seq


title: cy80_CD_90_pos_A04_S868_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_B10_S22_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_A06_S6_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_C06_S318_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_B04_S16_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_4_G06_S78_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_E07_S439_comb

description: single cell RNA-seq


title: cy88_cd_45_pos_G09_S465_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_G11_S467_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_F04_S352_comb

description: single cell RNA-seq


title: cy80_CD_90_pos_F08_S932_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_C08_S416_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_D06_S426_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_HC_pos_HLDAR_pos_A02_S962_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_D09_S429_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_G07_S463_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_H06_S474_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_D03_S423_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_C11_S419_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_B06_S402_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_G08_S464_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_G10_S466_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_G03_S459_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_C10_S418_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_B12_S408_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_H03_S471_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_D08_S428_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_B05_S401_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_C09_S417_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_G12_S468_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_E10_S442_comb

description: single cell RNA-seq


title: cy60_1_cd_45_pos_3_E10_S346_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_H07_S475_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_C04_S412_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_D01_S421_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_C03_S411_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_H01_S469_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_A01_S385_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_A11_S395_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_G09_S465_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_G05_S461_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_C07_S415_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_H10_S478_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_H11_S479_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_B10_S406_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_E09_S441_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_D12_S432_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_A10_S394_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_B02_S398_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_B09_S405_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_D11_S431_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_A04_S388_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_E04_S436_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_A12_S396_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_D10_S430_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_B07_S403_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_D02_S422_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_E01_S433_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_H02_S470_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_E06_S438_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_G04_S460_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_B08_S404_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_E02_S434_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_C06_S414_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_H08_S476_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_D07_S427_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_A06_S390_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_G06_S462_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_B03_S399_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_H09_S477_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_E05_S437_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_E08_S440_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_B04_S400_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_H12_S480_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_C05_S413_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_E03_S435_comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-H09-S765-comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_A03_S387_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_B11_S407_comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_G02_S458_comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-E04-S724-comb

description: single cell RNA-seq


title: cy84-Primary-CD45-neg-D05-S1001-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-C04-S508-comb

description: single cell RNA-seq


title: cy80-CD45-neg-E07-S439-comb

description: single cell RNA-seq


title: cy80-CD45-neg-D05-S425-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-G12-S756-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-A10-S682-comb

description: single cell RNA-seq


title: cy84-Met-CD45-pos-F11-S935-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-E09-S537-comb

description: single cell RNA-seq


title: cy84-Met-CD45-pos-B08-S884-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-E12-S732-comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_C01_S409_comb

description: single cell RNA-seq


title: cy84-Met-CD45-pos-H05-S953-comb

description: single cell RNA-seq


title: cy80-CD45-neg-D08-S428-comb

description: single cell RNA-seq


title: cy80-CD45-neg-B08-S404-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-H10-S574-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-A07-S679-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-F11-S743-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-A04-S676-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-F07-S547-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-C03-S507-comb

description: single cell RNA-seq


title: cy80-CD45-neg-G07-S463-comb

description: single cell RNA-seq


title: cy80-CD45-neg-E06-S438-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-B11-S695-comb

description: single cell RNA-seq


title: cy84-Met-CD45-pos-A11-S875-comb

description: single cell RNA-seq


title: cy80-CD45-neg-F08-S452-comb

description: single cell RNA-seq


title: cy80-CD45-neg-F11-S455-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-D06-S522-comb

description: single cell RNA-seq


title: cy84-Met-CD45-pos-A04-S868-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-G01-S553-comb

description: single cell RNA-seq


title: cy84_Primary_CD45_pos_A09_S393_comb

description: single cell RNA-seq


title: cy80-CD45-neg-A03-S387-comb

description: single cell RNA-seq


title: cy84-Met-CD45-pos-G09-S945-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-E10-S730-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-G06-S750-comb

description: single cell RNA-seq


title: cy84-Met-CD45-pos-D06-S906-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-G02-S554-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-A08-S680-comb

description: single cell RNA-seq


title: cy84-Primary-CD45-neg-A07-S967-comb

description: single cell RNA-seq


title: cy84-Primary-CD45-neg-F05-S1025-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-B04-S688-comb

description: single cell RNA-seq


title: cy84-Met-CD45-pos-G07-S943-comb

description: single cell RNA-seq


title: cy78-CD45-neg-2-B07-S595-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-D06-S714-comb

description: single cell RNA-seq


title: cy78-CD45-neg-2-A08-S584-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-C07-S511-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-A03-S675-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-C03-S699-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-D12-S720-comb

description: single cell RNA-seq


title: cy84-Primary-CD45-neg-H12-S1056-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-F08-S740-comb

description: single cell RNA-seq


title: cy80-CD45-neg-D11-S431-comb

description: single cell RNA-seq


title: cy80-CD45-neg-F07-S451-comb

description: single cell RNA-seq


title: cy80-CD45-neg-B04-S400-comb

description: single cell RNA-seq


title: cy84-Met-CD45-pos-H04-S952-comb

description: single cell RNA-seq


title: cy84-Met-CD45-pos-A01-S865-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-C10-S706-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-A06-S486-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-A09-S681-comb

description: single cell RNA-seq


title: cy84-Met-CD45-pos-F04-S928-comb

description: single cell RNA-seq


title: cy84-Met-CD45-pos-C07-S895-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-F10-S742-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-H12-S576-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-H08-S764-comb

description: single cell RNA-seq


title: cy84-Primary-CD45-neg-H07-S1051-comb

description: single cell RNA-seq


title: cy84-Primary-CD45-neg-A12-S972-comb

description: single cell RNA-seq


title: cy84-Primary-CD45-neg-H02-S1046-comb

description: single cell RNA-seq


title: cy80-CD45-neg-D09-S429-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-B12-S504-comb

description: single cell RNA-seq


title: cy78-CD45-neg-2-A10-S586-comb

description: single cell RNA-seq


title: cy80-CD45-neg-H01-S469-comb

description: single cell RNA-seq


title: cy80-CD45-neg-F05-S449-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-G03-S747-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-B05-S689-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-D11-S719-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-G05-S749-comb

description: single cell RNA-seq


title: cy84-Met-CD45-pos-F10-S934-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-G08-S752-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-C05-S509-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-F11-S551-comb

description: single cell RNA-seq


title: cy80-CD45-neg-B12-S408-comb

description: single cell RNA-seq


title: cy80-CD45-neg-C07-S415-comb

description: single cell RNA-seq


title: cy80-CD45-neg-E01-S433-comb

description: single cell RNA-seq


title: cy78-CD45-neg-2-B05-S593-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-A11-S683-comb

description: single cell RNA-seq


title: cy80-CD45-neg-C01-S409-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-D04-S520-comb

description: single cell RNA-seq


title: cy84-Met-CD45-pos-A02-S866-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-H04-S760-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-G10-S754-comb

description: single cell RNA-seq


title: cy80-CD45-neg-A06-S390-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-C06-S510-comb

description: single cell RNA-seq


title: cy84-Primary-CD45-neg-A10-S970-comb

description: single cell RNA-seq


title: cy80-CD45-neg-G08-S464-comb

description: single cell RNA-seq


title: cy84-Met-CD45-pos-B04-S880-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-F03-S735-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-C04-S700-comb

description: single cell RNA-seq


title: cy84-Met-CD45-pos-G01-S937-comb

description: single cell RNA-seq


title: cy80-CD45-neg-C04-S412-comb

description: single cell RNA-seq


title: cy80-CD45-neg-E10-S442-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-G01-S745-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-B10-S694-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-F04-S736-comb

description: single cell RNA-seq


title: cy78-CD45-neg-2-A05-S581-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-C01-S505-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-F01-S733-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-H05-S569-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-F05-S737-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-A06-S678-comb

description: single cell RNA-seq


title: cy78-CD45-neg-2-C07-S607-comb

description: single cell RNA-seq


title: cy78-CD45-neg-2-B11-S599-comb

description: single cell RNA-seq


title: cy80-CD45-neg-C05-S413-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-H02-S758-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-A11-S491-comb

description: single cell RNA-seq


title: cy80-CD45-neg-E02-S434-comb

description: single cell RNA-seq


title: cy84-Primary-CD45-neg-E05-S1013-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-F09-S741-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-E07-S727-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-G12-S564-comb

description: single cell RNA-seq


title: cy84-Met-CD45-pos-H09-S957-comb

description: single cell RNA-seq


title: cy78-CD45-neg-2-B10-S598-comb

description: single cell RNA-seq


title: cy80-CD45-neg-H07-S475-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-H03-S567-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-B06-S498-comb

description: single cell RNA-seq


title: cy80-CD45-neg-D01-S421-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-E06-S726-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-D01-S517-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-B01-S685-comb

description: single cell RNA-seq


title: cy80-CD45-neg-D04-S424-comb

description: single cell RNA-seq


title: cy80-CD45-neg-E09-S441-comb

description: single cell RNA-seq


title: cy80-CD45-neg-A11-S395-comb

description: single cell RNA-seq


title: cy78-CD45-neg-2-A06-S582-comb

description: single cell RNA-seq


title: cy78-CD45-neg-2-A12-S588-comb

description: single cell RNA-seq


title: cy84-Met-CD45-pos-B02-S878-comb

description: single cell RNA-seq


title: cy80-CD45-neg-F10-S454-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-F07-S739-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-A12-S684-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-B03-S687-comb

description: single cell RNA-seq


title: cy80-CD45-neg-G02-S458-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-C01-S697-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-D12-S528-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-B11-S503-comb

description: single cell RNA-seq


title: cy80-CD45-neg-F04-S448-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-F12-S744-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-A05-S677-comb

description: single cell RNA-seq


title: cy80-CD45-neg-C12-S420-comb

description: single cell RNA-seq


title: cy80-CD45-neg-A12-S396-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-F05-S545-comb

description: single cell RNA-seq


title: cy84-Primary-CD45-neg-A03-S963-comb

description: single cell RNA-seq


title: cy84-Met-CD45-pos-B07-S883-comb

description: single cell RNA-seq


title: cy80-CD45-neg-B05-S401-comb

description: single cell RNA-seq


title: cy80-CD45-neg-H03-S471-comb

description: single cell RNA-seq


title: cy80-CD45-neg-A10-S394-comb

description: single cell RNA-seq


title: cy80-CD45-neg-G01-S457-comb

description: single cell RNA-seq


title: cy80-CD45-neg-E04-S436-comb

description: single cell RNA-seq


title: cy80-CD45-neg-D07-S427-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-H02-S566-comb

description: single cell RNA-seq


title: cy84-Primary-CD45-neg-G07-S1039-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-B08-S692-comb

description: single cell RNA-seq


title: cy80-CD45-neg-C08-S416-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-C09-S705-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-D09-S717-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-D09-S525-comb

description: single cell RNA-seq


title: cy78-CD45-neg-2-C05-S605-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-G04-S556-comb

description: single cell RNA-seq


title: cy84-Primary-CD45-neg-E12-S1020-comb

description: single cell RNA-seq


title: cy84-Primary-CD45-neg-G03-S1035-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-D05-S521-comb

description: single cell RNA-seq


title: cy80-CD45-neg-D02-S422-comb

description: single cell RNA-seq


title: cy80-CD45-neg-D03-S423-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-D08-S716-comb

description: single cell RNA-seq


title: cy80-CD45-neg-F12-S456-comb

description: single cell RNA-seq


title: cy80-CD45-neg-A05-S389-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-F08-S548-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-B08-S500-comb

description: single cell RNA-seq


title: cy80-CD45-neg-B06-S402-comb

description: single cell RNA-seq


title: cy80-CD45-neg-G12-S468-comb

description: single cell RNA-seq


title: cy84-Met-CD45-pos-H10-S958-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-G09-S753-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-D07-S715-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-E05-S725-comb

description: single cell RNA-seq


title: cy84-Met-CD45-pos-B05-S881-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-F10-S550-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-C10-S514-comb

description: single cell RNA-seq


title: cy80-CD45-neg-F06-S450-comb

description: single cell RNA-seq


title: cy84-Met-CD45-pos-D03-S903-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-G07-S751-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-H08-S572-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-H04-S568-comb

description: single cell RNA-seq


title: cy84-Met-CD45-pos-F09-S933-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-A04-S484-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-F02-S542-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-D02-S710-comb

description: single cell RNA-seq


title: cy80-CD45-neg-B11-S407-comb

description: single cell RNA-seq


title: cy84-Met-CD45-pos-G05-S941-comb

description: single cell RNA-seq


title: cy80-CD45-neg-H11-S479-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-A03-S483-comb

description: single cell RNA-seq


title: cy80-CD45-neg-A07-S391-comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-B02-S494-comb

description: single cell RNA-seq


title: cy78-CD45-neg-3-G02-S746-comb

description: single cell RNA-seq


title: CY88_3_G06_S654_comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-H07-S571-comb

description: single cell RNA-seq


title: CY88_5_D01_S709_comb

description: single cell RNA-seq


title: CY88_5_H07_S763_comb

description: single cell RNA-seq


title: CY88_3_F04_S640_comb

description: single cell RNA-seq


title: CY88_5_F10_S742_comb

description: single cell RNA-seq


title: CY88_3_E11_S635_comb

description: single cell RNA-seq


title: CY88_5_G11_S755_comb

description: single cell RNA-seq


title: CY88_5_C03_S699_comb

description: single cell RNA-seq


title: CY88_5_G10_S754_comb

description: single cell RNA-seq


title: CY88_5_H11_S767_comb

description: single cell RNA-seq


title: CY88_5_E10_S730_comb

description: single cell RNA-seq


title: CY88_3_A01_S577_comb

description: single cell RNA-seq


title: CY88_3_C02_S602_comb

description: single cell RNA-seq


title: cy78-CD45-neg-1-F06-S546-comb

description: single cell RNA-seq


title: CY88_5_H01_S757_comb

description: single cell RNA-seq


title: CY88_5_C04_S700_comb

description: single cell RNA-seq


title: CY88_5_E08_S728_comb

description: single cell RNA-seq


title: CY88_5_C07_S703_comb

description: single cell RNA-seq


title: CY88_5_C10_S706_comb

description: single cell RNA-seq


title: CY88_3_C09_S609_comb

description: single cell RNA-seq


title: CY88_5_B05_S689_comb

description: single cell RNA-seq


title: cy78-CD45-neg-2-C08-S608-comb

description: single cell RNA-seq


title: CY88_5_B09_S693_comb

description: single cell RNA-seq


title: CY88_3_H03_S663_comb

description: single cell RNA-seq


title: CY88_5_B04_S688_comb

description: single cell RNA-seq


title: CY88_5_G12_S756_comb

description: single cell RNA-seq


title: CY88_5_A10_S682_comb

description: single cell RNA-seq


title: VCaP-siUBE2I-ChIPseq-Pol2-HS-rep2

status: Public on Jul 02 2015

city: Kuopio

first: Einari

middle: A

last: Niskanen

address: Yliopistonranta 8

department: School of Medicine

country: Finland

institute: University of Eastern Finland

description:
ChIP-seq
processed data file: VCaP_siUBE2I_Pol2_HS.bedGraph.gz

library_source: genomic

data_processing:
The quality of raw reads was analyzed by FastQC and FASTX-toolkit was used for preprocessing raw reads (removal of artifacts, trimming reads, quality filtering and collapsing identical reads).
Reads were aligned against human genome hg19 with Bowtie software (0.12.7) with the command line: -v 1 -k 1 -m 1 -f -S --best hg19.
BedGraph files were done using HOMER software (V 4.6; http://homer.salk.edu/homer/) by combining two replicates and normalizing total number of reads to 10 million. GROseq signal is divided between plus- and minus-strand signals in separate files.
Genome_build: hg19
Supplementary_files_format_and_content: ChIP-seq signal intensities as bedGraph files.

molecule: genomic DNA

source_name: VCaP cells

growth_protocol: VCaP cells were grown in DMEM medium supplemented with 10% feta bovine serum and 1% penicillin/streptomycin antibiotics at 37 degree C temperature incubator with 5% CO2.

extract_protocol:
Nuclei from approximately 10 million formaldehyde crosslinked (1%; 10min at room temperature for VCaP cells, and 8min. at room temperature for K562 cells)  cells were isolated and chromatin was fragmented using sonicator (bioruptor). Lysates were cleared and protein-DNA complexes were isolated using target specific antibodies and protein-A/G magnetic beads.
DNA fragments were purified and libraries were prepared accorcing to standard Illumina protocol. Samples were sequenced at BGI Tech Solutions (Shenzhen, China) or at the Gene Core in EMBL (Heidelberg, Germany).

treatment_protocol:
Heat shock for ChIP-seq samples was done at 43 degree C incubator for 30min.
Silencing for VCaP cells was done by transfecting siRNA for UBE2I (siUBE2I samples), PIAS1 (siPIAS1 samples) or non-targeting control siRNA (siNON samples) using lipofectamine RNAiMAX transfection reagent (life technologies).
RNA polymerase II inhibitors TRP (triptolide; 1 micromolar) and DRB (5,6-Dichlorobenzimidazole 1-β-D-ribofuranoside, 100 micromolar) were applied to VCaP cells 3h before collecting.

tag: cell line

value: VCaP

value: heat shock (30min at 43 degree C)

value: UBE2I silenced (siUBE2I transfected)

tag: chip antibody

value: anti-Pol2, clone:8WG16 (Covance, catalog# MMS-126R, lot# E12CF00481)


title: CY88_3_E06_S630_comb

description: single cell RNA-seq


title: VCaP-siUBE2I-ChIPseq-Pol2-HS-rep1


title: VCaP-siUBE2I-ChIPseq-Pol2-C-rep1

description:
ChIP-seq
processed data file: VCaP_siUBE2I_Pol2_C.bedGraph.gz

value: control (37 degree C)


title: CY88_5_H02_S758_comb

description: single cell RNA-seq


title: VCaP-siUBE2I-ChIPseq-Pol2-C-rep2


title: H3K4me3

status: Public on May 26 2015

city: Worcester

first: Troy

middle: W.

last: Whitfield

address: 55 Lake Ave. N.

country: USA

institute: University of Massachusetts Medical School

data_processing:
Base calls and sequence reads were generated by Illumina CASAVA software (version 1.6, Illumina).
Reads were mapped to the human genome (GRCh37, hg19) using bowtie (version 0.12.8) with up to two mismatches allowed.
Peak calling was performed on pooled replicates using MACS (version 2.0.10.20131216) with default settings and retaining peaks with a p < 10^{-20} threshold.
Genome_build: hg19
Supplementary_files_format_and_content: Bed files correspond to summits (+/- 50 bp) for peaks with p < 10^{-20}.

growth_protocol: Kasumi-1 cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA) and maintained in RPMI-1640 media supplemented with 20% FBS. Cells were expanded every 3-4 days in T75 tissue culture flasks. Kasumi-1 cells growing in log phase (1-1.5 million cells/ml) were fixed with 1% formaldehyde for 10 minutes at room temperature and subsequently quenched with 0.25 M glycine. Approximately 30 million cells were used for each ChIP-Seq library.

extract_protocol:
Kasumi-1 cells growing in log phase were fixed with 1% formaldehyde for 10 minutes at room temperature and subsequently quenched with 0.25 M glycine. After washes in PBS, cells were flash frozen in liquid nitrogen. Thawed pellets were resuspended in Buffer A (50 mM HEPES, 140 mM NaCl, 1 mM EDTA, 10% glycerol, 0.5% NP-40, 0.25% Triton X-100) and rotated at 4ºC for 10 minutes. Following centrifugation, pellets were resuspended in Buffer B (10 mM Tris-HCl, 200 mM NaCl, 1 mM EDTA, 1 mM EGTA) and rotated at 4ºC for 10 minutes. Pellets from centrifuged samples were resuspended in Buffer C (10 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, 1 mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine). Samples were aliquoted, 3 ml per tube, and sonicated to a fragment size of 100-500 base pairs using a 3.2 mm sonication probe (QSonica, Newtown, CT). Sheared chromatin (from approximately 30 million cells) was immunoprecipitated with antibodies overnight. All ChIP experiments included either normal goat or rabbit IgG (EMD Millipore, Billerica, MA) as controls. Antibody-lysate complexes were mixed with Protein G Dynabeads (Life Technologies, Grand Island, NY) for 2 hours. For AML1, ETO, H3K4me3, and H3K27me3 immunoprecipitations, beads were washed once with IP buffer, 3 times with RIPA buffer (50 mM HEPES, 500 mM LiCl, 1 mM EDTA, 0.5% NP-40, 0.25% sodium deoxycholate), once with PBS, and once with TE buffer. For N-CoR and p300 pulldowns, beads were washed once with IP buffer, once with high salt buffer (2 mM EDTA, 20 mM Tris-HCl, 500 mM NaCl), 1 time with RIPA buffer (50 mM HEPES, 250 mM LiCl, 1 mM EDTA, 0.5% NP-40, 0.25% sodium deoxycholate), once with PBS, and once with TE buffer. Protein-DNA complexes were extracted from beads at 37ºC in elution buffer (10 mM EDTA, 50 mM Tris-HCl, 1% SDS). For reverse crosslinking, supernatants from centrifuged samples were rotated overnight at 60ºC. RNAse and proteinase K treated samples were extracted with phenol:chloroform:isoamyl alcohol. Precipitated DNA was resuspended in 10 mM Tris-HCl, quantified with a Qubit fluorometer (Life Technologies, Grand Island, NY).
The Illumina protocol (Illumina, Inc., San Diego, CA) for ChIP-seq library generation was used with slight modifications. Approximately 5-10 ng chromatin was end-repaired (EpiCentre Biotechnologies, Madison, WI). Material was then A-tailed and ligated with adapters for single end deep sequencing (Illumina, Inc., San Diego, CA). Adapter modified DNA was size-selected, 300-400 base pair (bp) range, and then amplified using the Phusion polymerase (New England Biolabs, Ipswich, MA). Amplified ChIP libraries were size selected and sequenced on an Illumina GAIIx Genome Analyzer (Illumina, Inc., San Diego, CA) at the UMass Medical School Deep Sequencing Core Facility (Worcester, MA).

tag: tissue origin

value: peripheral blood

tag: cell type

value: myeloblast

tag: passages

tag: chip antibody vendor

value: Abcam

tag: chip antibody cat. #

tag: chip antibody lot #


title: VCaP-siNON-ChIPseq-Pol2-C-rep1

description:
ChIP-seq
processed data file: VCaP_siNON_Pol2_C.bedGraph.gz

value: control for siUBE2I (control siRNA transfected)


title: VCaP-siNON-ChIPseq-Pol2-HS-rep2

description:
ChIP-seq
processed data file: VCaP_siNON_Pol2_HS.bedGraph.gz


title: E13.5_Male_PGC_KO_PBAT

status: Public on Sep 01 2014

city: Vancouver

first: Matthew

last: Lorincz

state: BC

address: 2350 Health Sciences Mall

department: Medical Genetics

country: Canada

institute: University of British Columbia

data_processing:
Alignment of PBAT data and methylation calling were performed using Bismark (Krueger and Andrews 2011).
Sample_processed_data_files_format_and_content: BigWig files having % of methylation.

organism: Mus musculus

source_name: primordial germ cells

extract_protocol:
Primordial germ cells (SSEA1+) and Somatic gonadal cells (SSEA-) were isolated from the gonads of E13.5 embryos and purified by FACS. Cells were sorted directly in nuclear isolation buffer containing RNAse inhibitor and protease inhibitor cocktail. For RNA-seq libraries, RNA was extracted using TriReagent, and dsCDNA was synthesized using Superscript III and Klenow fragment, respectively. For ChIP-seq libraries, DNA was extracted from immunoprecipitated DNA-protein complexes using phenol:chloroform:isoamyl alcohol 25:24:1.
Adaptor ligation based library construction for paired-end Illumina sequencing.

value: SSEA1+ primordial germ cells

tag: dev. stage

tag: genotype

value: Setdb1 delta/- (KO)

tag: Sex

value: male


title: VCaP-siNON-ChIPseq-Pol2-HS-rep1


title: VCaP-siNON-ChIPseq-Pol2-C-rep2


title: E13.5_Female_PGC_Het_H3K9me3

description: index: CGATTA

data_processing:
Alignment (ChIP-seq): Sequence reads were mapped to mm9 (NCBI 37) using BWA (Li and Durbin 2009) and default parameters.
Wigs (ChIP-seq): Reads having identical coordinates were collapsed into a single read and reads with mapQ >=5 passed to FindPeaks 3.1 (Fejes et al., 2008) (with a fixed directional read extension of 150bp) for  visualization.
Sample_processed_data_files_format_and_content: BigWig files having raw data.
Sample_processed_data_files_format_and_content: RPKMs for Ensembl gene promoters (TSS +/- 1KB) and different TE families.

value: Setdb1 flox/- (HET)

value: female

tag: antibody

value: H3K9me3 (Active Motif #39161)


title: E13.5_Female_PGC_KO_H3K9me3

description: index: ACATCG


title: E13.5_Female_PGC_Het_H3K27me3

description: index: CATTCA

value: H3K27me3 (Diagenode #pAb-069-050)


title: E13.5_Female_PGC_KO_H3K27me3

description: index: AAGCTA


title: E13.5_Male_PGC_Het_H3K9me3

description: index: CTGATC


title: E13.5_Male_PGC_Het_PBAT


title: E13.5_Male_PGC_KO_H3K27me3

description: index: CTTTTG


title: E13.5_Male_PGC_Het_H3K27me3

description: index: GGGGTT


title: CY88_5_D04_S712_comb

description: single cell RNA-seq


title: E13.5_Female_PGC_KO_RNAseq_rep2

description: index: GGAACT

data_processing:
Alignment (RNA-seq): Sequence reads were aligned to the mouse reference genome mm9 (NCBI 37) using BWA (Li and Durbin 2009), with annotated exon-exon junctions compiled from Ensembl, RefSeq and UCSC gene annotation sources, as described (Morin et al., 2010; Shah et al., 2009). Sequence reads that could be uniquely assigned a position in the transcript resource (exon-exon junctions) were computationally repositioned to the genomic mm9 coordinates and a single merged bam file (Li et al., 2009) generated for downstream analyses.
Wigs (RNA-seq): The Samtools pileup utility (Li et al., 2009) and FindPeaks 3.1 (Fejes et al., 2008) was used to generate data tracks for visualization (wig and bigWig (Kent et al., 2010)) in the UCSC browser. Repbase (Jurka et al., 2005), a comprehensive database of repetitive elements, was used for alignment of reads to repetitive elements.
Sample_processed_data_files_format_and_content: BigWig files having raw data.
Sample_processed_data_files_format_and_content: RPKMs for all protein coding ensemble genes and different TE families.

molecule: total RNA


title: E13.5_Male_PGC_WT_pan-H3

description: index: GCGTGG

value: wildtype (C57BL/6)

value: pan-H3 (Sigma #I8140)


title: E13.5_Female_PGC_KO_RNAseq_rep1

description: index: GGGGTT


title: E13.5_Male_Soma_WT_H3K9me3

description: index: AGCGCT

source_name: somatic gonadal cells

value: SSEA1- gonadal somatic cells


title: H3K27me3

value: EMD Millipore


title: Control

value: input


title: E13.5_Female_PGC_Het2_RNAseq_rep2

description: index: CGATTA

value: Setdb1 flox/- (HET2)


title: E13.5_Female_PGC_Het2_RNAseq_rep1

description: index: AGCATC


title: E13.5_Female_PGC_Het1_RNAseq_rep2

description: index: AAGCTA

value: Setdb1 delta/+ (HET1)


title: E13.5_Female_PGC_Het1_RNAseq_rep1

description: index: AGCTAG


title: E13.5_Male_PGC_KO_RNAseq_rep1

description: index: TGTTGG


title: E13.5_Female_PGC_WT_RNAseq_rep1

description: index: GCCGGT

value: Setdb1 flox/+ (WT)


title: E13.5_Male_PGC_KO_H3K9me3

description: index: AGCGCT


title: E13.5_Male_PGC_KO_RNAseq_rep2

description: index: GGGGGT


title: E13.5_Male_PGC_Het2_RNAseq_rep2

description: index: CTGATC


title: E13.5_Male_Soma_C57BL/6_RNAseq

description: index: AGCATC

data_processing:
Alignment (RNA-seq): Sequence reads were aligned to the mouse reference genome mm9 (NCBI 37) using BWA (Li and Durbin 2009), with annotated exon-exon junctions compiled from Ensembl, RefSeq and UCSC gene annotation sources, as described (Morin et al., 2010; Shah et al., 2009). Sequence reads that could be uniquely assigned a position in the transcript resource (exon-exon junctions) were computationally repositioned to the genomic mm9 coordinates and a single merged bam file (Li et al., 2009) generated for downstream analyses.
Wigs (RNA-seq): The Samtools pileup utility (Li et al., 2009) and FindPeaks 3.1 (Fejes et al., 2008) was used to generate data tracks for visualization (wig and bigWig (Kent et al., 2010)) in the UCSC browser. Repbase (Jurka et al., 2005), a comprehensive database of repetitive elements, was used for alignment of reads to repetitive elements.
Sample_processed_data_files_format_and_content: BigWig files having raw data.
Sample_processed_data_files_format_and_content: RPKMs for all protein coding ensemble genes.


title: E13.5_Male_PGC_WT_RNAseq_rep1

description: index: AGCTAG


title: E13.5_Male_PGC_Het1_RNAseq_rep2

description: index: CTTTTG


title: E13.5_Male_PGC_Het2_RNAseq_rep1

description: index: AGCGCT


title: E13.5_Male_PGC_C57BL/6_RNAseq

description: index: AGCTAG


title: Stanford_ChipSeq_ES-E14_Input_std (superseded by GSE136425)

status: Public on Sep 13 2012

city: Stanford

first: ENCODE

last: DCC

state: CA

address: 300 Pasteur Dr

institute: ENCODE DCC

description:
****************************
GSM1003746 record metadata submitted on Sep 13 2012 may be obsolete, up to date experimental metadata was resubmitted by ENCODE DCC and is available at GSE136425
****************************

extract_protocol: Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=mm9&g=wgEncodeSydhHist

tag: lab

value: Stanford-m

tag: lab description

value: Snyder - Stanford University

tag: datatype

value: ChipSeq

tag: datatype description

value: Chromatin IP Sequencing

tag: cell

tag: cell organism

value: mouse

tag: cell description

value: mouse embryonic stem cell line E14

tag: cell sex

value: M

value: Input

tag: antibody description

value: Control signal which may be subtracted from experimental raw signal before peaks are called.

tag: treatment

tag: treatment description

value: No special treatment or protocol applies

tag: control

value: std

tag: control description

value: Standard input signal for most experiments.

tag: age

tag: age description

value: Embryonic day 0 (stem cell)

tag: controlid

tag: replicate

tag: strain

tag: strain description

value: The mice have yellow coats and late onset severe vacuolation in brain (700+ days). Inbred strain used as a control group to compare with null PrP mice.


title: E13.5_Male_PGC_Het1_RNAseq_rep1

description: index: CTGGGT

tag: cell type

value: SSEA1+ primordial germ cells

tag: dev. stage

tag: genotype

tag: Sex

value: male


title: USC_ChipSeq_GM08714_Input_UCDavis (superseded by GSE136428)

status: Public on May 22 2012

description:
****************************
GSM935607 record metadata submitted on May 22 2012 may be obsolete, up to date experimental metadata was resubmitted by ENCODE DCC and is available at GSE136428
****************************

extract_protocol: Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeSydhTfbs

tag: lab

value: USC

tag: lab description

value: Farnham - University of Southern California

tag: datatype

tag: datatype description

tag: cell

tag: cell organism

value: human

tag: cell description

value: lymphoblastoid cell line, Instability of heterochromatin of chromosomes 1, 9, and 16 with variable combined immunodeficiency; dysmorphic facial features, developmental delay, malabsorption, and recurrent infections; see GM08747 Fibroblast; donor subject is a compound heterozygote:one allele has a G>A transition at nucleotide 1807 (1807G>A) of the DNMT3B gene resulting in an Ala to Thr substitution at codon 603 [Ala603Thr (A603T)], the mutation occurring in a region between motifs I and IV within the catalytic domain of DNMT3B; the second allele has a G>A transition within intron 22 located 11 nucleotides 5-prime of the normal splice acceptor site [IVS22AS,G>A,-11] resulting in the generation of a novel splice acceptor site and a 9-bp insertion in the processed RNA. This results in the insertion of 3 amino acids (serine, threonine, and proline) at codon 744 (744ins3). The insertion was within the conserved region of the catalytic domain, which is likely to be disrupted by the insertion of a proline residue. This mutation was de novo.

tag: cell lineage

value: mesoderm

value: F

tag: antibody

value: UCDavis

value: Input library was prepared at UC Davis.


title: Stanford_ChipSeq_CH12_Input_IgG-rab (superseded by GSE136427)

status: Public on Apr 11 2012

description:
****************************
GSM912917 record metadata submitted on Apr 11 2012 may be obsolete, up to date experimental metadata was resubmitted by ENCODE DCC and is available at GSE136427
****************************

value: Stanford-m

value: Snyder - Stanford University

value: ChipSeq

value: Chromatin IP Sequencing

value: mouse

value: B-cell lymphoma (GM12878 analog)

tag: cell sex

value: Input

tag: antibody description

tag: treatment

tag: treatment description

tag: control

value: IgG-rab

tag: control description

value: Input signal from Normal Rabbit IgG ChIP-seq.

tag: age

value: immortalized

tag: age description

value: Immortal cells

tag: controlid

value: Derived by inbreeding from selected F2 progeny of B10.A X B10.129


title: USC_ChipSeq_HEK293_Input_UCDavis (superseded by GSE136429)

description:
****************************
GSM935586 record metadata submitted on May 22 2012 may be obsolete, up to date experimental metadata was resubmitted by ENCODE DCC and is available at GSE136429
****************************

value: immortalized cell line

tag: lab

value: USC

tag: lab description

value: Farnham - University of Southern California

tag: line

value: human

value: embryonic kidney, cells contain Adenovirus 5 DNA (PMID:11967234)

tag: cell karyotype

value: normal

value: U

value: No special treatment or protocol applies

value: Control signal which may be subtracted from experimental raw signal before peaks are called.


title: ChIP-Seq analysis of WCE in human Mobilized CD34 cells; DNA_Lib 253

status: Public on Dec 28 2011

city: Cambridge

first: BROAD

last: INSTITUTE

state: MA

institute: Broad Institute

description:
sample_term_id: CL_0000837
assay_term_id: OBI_0000716
nucleic_acid_term_id: SO_0000352
Design description: Human Chromatin IP REMC Sequencing on Illumina
Library name: DNA_Lib 253
EDACC Genboree Experiment Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FBroad%2FEXPERIMENT%2FEDACC.8640
EDACC Genboree Sample Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FUniversity%20of%20Washington%2FSAMPLE%2FEDACC.2397
****************
For data usage terms and conditions, please refer to:
http://www.drugabuse.gov/funding/funding-opportunities/nih-common-fund/epigenomics-data-access-policies
****************

data_processing:
**********************************************************************

ANALYSIS FILE NAME: GSM772950_BI.Mobilized_CD34_Primary_Cells.Input.RO_01536.bed
ANALYSIS CENTER: EDACC
ANALYSIS ALIAS: DNA_Lib 253-DNA_Lib 253-DNA_Lib 253-DNA_Lib 253-DNA_Lib 253.hg19.level.1.release.5
ANALYSIS TITLE: Mapping of Mobilized CD34 Primary Cells ChIP-Seq Input Data
ANALYSIS DESCRIPTION: Illumina reads produced by ChIP-Seq Input on Mobilized CD34 Primary Cells, Donor RO 01536, were mapped to the human genome using Pash.
ANALYSIS TYPE: REFERENCE_ALIGNMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.10616
DATA_ANALYSIS_LEVEL: 1
EXPERIMENT_TYPE: ChIP-Seq
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19
SOFTWARE: Pash
SOFTWARE_VERSION: 3.0
MAXIMUM_ALIGNMENT_LENGTH: Read length
MISMATCHES_ALLOWED: 10% of read length
ALIGNMENTS_ALLOWED: 1
TREATMENT_OF_MULTIPLE_ALIGNMENTS: If a read maps to more than 1 position it is removed from consideration.
TREATMENT_OF_IDENTICAL_ALIGNMENTS_OF_MULTIPLE_READS: If multiple reads map to the same start position on the + strand or stop position on the - strand, only a single read is retained.
ALIGNMENT_POSTPROCESSING: None
READ_EXTENSION: 200bp
RELEASE_NUMBER: Human Epigenome Atlas 5

QUALITY SCORES:
NUMBER_OF_MAPPED_READS: 48,101,377
NUMBER_OF_Input_EXPERIMENTS_SCORED_IN_THIS_RELEASE: 56
FINDPEAKS_SCORE: 0.0047
FINDPEAKS_PERCENTILE: 34
HOTSPOT_SCORE: 0.0029
HOTSPOT_PERCENTILE: 5
IROC_SCORE: 1.0
IROC_PERCENTILE: 100
POISSON_SCORE: 0.018
POISSON_PERCENTILE: 3
MAXIMUM_REPLICATE_CORRELATION: 0.79

**********************************************************************

ANALYSIS FILE NAME: GSM772950_BI.Mobilized_CD34_Primary_Cells.Input.RO_01536.wig
ANALYSIS CENTER: EDACC
ANALYSIS ALIAS: DNA_Lib 253-DNA_Lib 253-DNA_Lib 253-DNA_Lib 253-DNA_Lib 253.hg19.level.2.release.5
ANALYSIS TITLE: Raw Signal Density Graphs of Mobilized CD34 Primary Cells ChIP-Seq Input Data
ANALYSIS DESCRIPTION: Illumina ChIP-Seq Input read mappings from Mobilized CD34 Primary Cells, Donor RO 01536, were processed into density graphs of raw signal representing the aligned read density.
ANALYSIS TYPE: ABUNDANCE_MEASUREMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.10880
DATA_ANALYSIS_LEVEL: 2
EXPERIMENT_TYPE: ChIP-Seq
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19
SOFTWARE: In house programs and scripts
SOFTWARE_VERSION: NA
READ_EXTENSION: 200bp
TREATMENT_OF_IDENTICAL_ALIGNMENTS_OF_MULTIPLE_READS: If multiple reads map to the same start position on the + strand or stop position on the - strand, only a single read is retained.
GENOMIC_WINDOW: 20bp
TREATMENT_OF_REGIONS_PRONE_TO_MULTIPLE_ALIGNMENTS: None
RELEASE_NUMBER: Human Epigenome Atlas 5
BROWSER_TRACK_NAME: MCD34 Input 36 53
BROWSER_TRACK_DESCRIPTION: BI Mobilized CD34 Primary Cells ChIP-Seq Input Donor RO 01536 Library DNA_Lib 253 EA Release 5

QUALITY SCORES:
NUMBER_OF_MAPPED_READS: 48,101,377
NUMBER_OF_Input_EXPERIMENTS_SCORED_IN_THIS_RELEASE: 56
FINDPEAKS_SCORE: 0.0047
FINDPEAKS_PERCENTILE: 34
HOTSPOT_SCORE: 0.0029
HOTSPOT_PERCENTILE: 5
IROC_SCORE: 1.0
IROC_PERCENTILE: 100
POISSON_SCORE: 0.018
POISSON_PERCENTILE: 3
MAXIMUM_REPLICATE_CORRELATION: 0.79

**********************************************************************

source_name: flow sorted CD34+ cells from cord blood; DNA_Lib 253

extract_protocol: Library construction protocol: Single read - Illumina

tag: sample alias

tag: sample common name

value: CD34 Primary Cells

tag: molecule

value: genomic DNA

tag: disease

value: FHCRC HEIMFELD

value: Primary Cell

tag: markers

value: 27 years

value: Female

value: Hispanic

value: standard

value: cell/nuclei lysis

value: ChIP-Seq Input

value: Branson