title: SMY2240_YAP6_t45

source_name: SMY2240 including YAP6 at the t45 minute timepoint


title: SMY2240_YAP6_t15

status: Public on Jan 03 2020

type: RNA

city: South San Francisco

first: Scott

last: McIsaac

state: CA

address: 1170 Veterans Blvd.

country: USA

institute: Calico Life Sciences LLC

hyb_protocol: 8x15k Microarray hybridization: Set up 60°C heat block; Prepare 10x Blocking Agent: Add 1250 µL of nuclease-free water to vial containing 10x lyophilized Gene Expression Blocking Agent (part #5188-5281); Vortex and spin down; Prepare hybridization samples; For 8x15k (8-pack) microarrays, mix the following components: 450 ng Cy3-amplified cRNA, 450 ng Cy5-amplified cRNA, 7.5 µL 10X Gene Expression Blocking Agent, bring volume to 36 µL with water, and then add 1.5 µL 25x fragmentation buffer; Incubate at 60°C for 30 minutes to fragment the RNA; Add 37.5 µL of 2x Hi-RPM Hybridization Buffer to strip tube and leave on ice; Add fragmented RNA directly to Hi-RPM Buffer to stop the reaction; Mix well by pipetting up and down (avoid bubbles and do not vortex); Put sample on ice and load onto array as soon as possible; Put clean gasket onto metal hybridization chamber; Add 45 µL of hybridization onto gasket; Place array (Agilent facing down) onto gasket and close the hybridization chamber; Incubate on rotisserie at 65°C for 17 hr

scan_protocol: Labeled RNA was hybridized to Agilent 8x15k microarrays, which were then washed, scanned, and processed using the Agilent Feature Extraction software with default settings and loess dye bias correction.

data_processing: Each microarray has some number of spots (usually 2) for each gene. All microarrays for the GEV experiments had identical probes, likewise for the ZEV experiments. For each spot, we computed ratio = max(red, C) / max(green, C), where C=2 in arbitrary units. This minimum value application only affects 0.3% of spots. Typical genes have red and green channel measurements above 200. These (red, green, ratio) values foreach spot serve as the raw data. For each dataset, we aggregated the data across individual spots. Specifically, for each timecourse, time point, and gene, we aggregated the spot values and measured the minimum value, maximum value, median value, and standard deviation of the values. In the usual case of 2 spots, the median value is equivalent to mean and standard deviation is equivalent to (max-min)/sqrt(2). At this stage we corrected the most extreme outlier observations. First, for a given sample, we examined the case where, for a given gene, the ratio of the spot values, was larger than four. Since the spots values do not agree, we interpolated the value with the geometric mean across bracketing time points (or neighboring time point in the case of first or last time point). The second class of outliers is where the median ratio moves by a factor of at least four between two time points, and then by a factor of four in the opposite direction for the next time point.  In these cases we again replaced the central point with the geometric mean of the bracketing points. These corrections apply to less than 0.2% of the data. In processing gene expression microarrays, crosstalk between red and green channels can occur. When the red channel fluorescence is much larger than the green channel fluorescence, there can be leakage of signal, and the green channel measurement is affected. To identify instances of this occurring,  we first computed the green channel ratio relative to the time zero measurement.  We then measured the 30% quantile of this value within each timecourse (for time points after t = 0), as well as the 30% quantile of the log-ratio.  We then flagged timecourses where the green ratio quantile exceeded a factor of eight, and the log-ratio quantile exceeds a two-fold change.  These represent cases where the green channel is increasing a great deal when it should be constant. For these rare cases, we repaired the red to green ratios by duplicating the time zero green channel across the full timecourse.  This affects about two dozen TF-gene timecourses (out of more than a million). The GEV and ZEV systems both have characteristic gene expression signatures, including a mild stress response. Previously, it has been shown that Singular Value Decomposition (SVD) is one way to remove such signals. Here, we used a slightly different approach. First, we computed the median time series for each gene in each class of experiments (GEV and ZEV).  We then subtracted this median time series, leaving a normalized log-ratio.  Because a given gene is not directly or indirectly affected by a transcription factor in most experiments, the median is an accurate reflection of any background time-dependent behavior.  We refer to the dataset where outliers are removed and the GEV/ZEV signal is removed as the cleaned dataset.

molecule: total RNA

organism: Saccharomyces cerevisiae

source_name: SMY2240 including YAP6 at the t15 minute timepoint

label_protocol: Crude RNA was purified using either the QIAGEN RNeasy kit or RNAClean Ampure XP beads. 200 ng of cleaned RNA was used as an input to generate dye-labeled cRNA using the Agilent Quick-Amp Labeling Kit. 1) Prepare labeling reaction: Get clean PCR strip tubes ready; Dilute RNA to 57.2 ng/µL; Add 3.5 µl of RNA (200 ng total) to PCR tube; Make T7 Primer Mix (0.8 µL T7, 1 µL water);Add 1.8 µl of T7 Primer Mix to each tube. Each tube now has 5.3 µl; Denature the template and primer by incubating at 65°C for 10 min; Put reactions on ice for 5 min; 2) cDNA synthesis: Heat 5x First Strand Buffer 80°C for 3-4 minutes, vortex, do a quick spin, and keep at room temperature; Make cDNA master mix (2µL 5x First Strand Buffer, 1 µL 0.1M DTT, 0.5 µL 10 mM dNTP mix, 1.2 µL Affinity Script RNase Block Mix); Briefly spin sample and master mix tubes; Add 4.7 µL of cDNA Master Mix to sample tubes and mix by pipetting up and down. Each tube now has 10 µL; Incubate at 40°C for 2 hours; Incubate at 70°C for 15 minutes (inactivates the AffinityScript enzyme); If you don’t continue to next step, freeze samples -80°C; Ice for 5 min. 3) Labeled cRNA synthesis: Keep T7 RNA polymerase (red cap) on ice. Master Mix = 0.75 µL Water, 3.2 µL 5x Transcription Buffer, 0.6 µL 0.1M DTT, 1 µL NTP Mix, .21µL T7 RNA polymerase Blend; 0.24 µL Cy3-CTP or C5-CTP; Add 6 µL of Transcrption Master Mix with Dye to the cDNA. Each tube now contains a total volume of 16 µL; Incubate samples at 40°C for 2 hours. 4) SPRI cleanup of cRNA: Before beginning: Bring RNAClean XP to RT, Make up enough 80% ethanol for >2 200 µL washes per reaction, Set 96 well thermomixer to 37°C; Add 48 µL (3X) of RNAClean XP beads to each reaction and incubate for 10 minutes; Place plate on magnet and allow supernatant to clear (~5 minutes); Remove supernatant carefully, avoiding beads, and discard; Add 200 µL of 80% ethanol to each well and incubate for 30 seconds; Remove ethanol and repeat for a total of two washes; Remove ethanol, cover plate, and spin down for ~1 minute; Place plate back on magnet and use a p20 to remove residual ethanol; Dry beads at 37°C until beads no longer move around when placed on magnet (~4 minutes); Add 42.5 of RNAse-free water and resuspend beads, then incubate for 2-3 minutes; Place plate on magnet and transfer 40 µL to a new well before placing on ice

growth_protocol: All experiments were performed in chemostats (continuous cultures). We chose chemostats, in part, because the steady-state condition of chemostat cultures is a particularly useful feature for mathematical modeling. Under steady-state conditions, the levels of molecules and activities of process are not changing at a culture-wide level. Cells were grown under continuous culturing conditions in 500 mL vessels as previously described with minor adjustments (Saldanha AJ, Brauer MJ, Botstein D. Nutritional homeostasis in batch and steady-state culture of yeast. Mol Biol Cell. 2004;15: 4089–4104). Cultures were aerated with 6 L/min of humidified air at 30C, maintained at 300 mL, and stirred with a magnetic impeller at 400 RPM. For the majority of experiments, cultures were maintained with minimal medium under phosphate limitation (20 mg/L). Nitrogen-limited cultures were maintained at 40 mg/L ammonium sulfate. Methionine-limited cultures (McIsaac et al, 2012) were maintained at 7.5 mg/L methionine. Growth rates were maintained from 0.15-0.17 vol/hour.  Batch growth in the chemostat vessels was initiated from a 1:60 dilution of a saturated overnight culture prior to turning on the chemostat pumps. Cells were grown to steady state, as determined by culture density, prior to the addition of 1 micromolar beta-estradiol to the culture and subsequent sampling. Chemostat experiments were performed with either the Infors Sixfors or Multifors systems.

extract_protocol: RNA was prepared using a standard acid phenol extraction. Lysis buffer: 600 µL of 0.5M EDTA, 1.5 mL of 10% SDS, 300 µL 1M Tris, 27.6 mL Rnase-free water.

treatment_protocol: The t = 0 was taken (between 3 and 5 mL of culture) and vacuum filtered. Estradiol was then added the continuous culture and sampled at various timepoints.

tag: strain

tag: background


title: SMY2240_YAP6_t20

source_name: SMY2240 including YAP6 at the t20 minute timepoint


title: SMY2240_YAP6_t10

source_name: SMY2240 including YAP6 at the t10 minute timepoint


title: SMY2263_SOK2_t30

source_name: SMY2263 including SOK2 at the t30 minute timepoint


title: SMY2240_YAP6_t0

source_name: SMY2240 including YAP6 at the t0 minute timepoint


title: SMY2237_WHI5_t45

source_name: SMY2237 including WHI5 at the t45 minute timepoint


title: SMY2237_WHI5_t30

source_name: SMY2237 including WHI5 at the t30 minute timepoint


title: SMY2237_WHI5_t10

source_name: SMY2237 including WHI5 at the t10 minute timepoint


title: SMY2240_YAP6_t30

source_name: SMY2240 including YAP6 at the t30 minute timepoint


title: SMY2152_GRX4_t90

source_name: SMY2152 including GRX4 at the t90 minute timepoint


title: SMY2237_WHI5_t90

source_name: SMY2237 including WHI5 at the t90 minute timepoint


title: SMY2237_WHI5_t5

source_name: SMY2237 including WHI5 at the t5 minute timepoint


title: SMY2237_WHI5_t0

source_name: SMY2237 including WHI5 at the t0 minute timepoint


title: SMY2237_WHI5_t20

source_name: SMY2237 including WHI5 at the t20 minute timepoint


title: SMY2240_YAP6_t5

source_name: SMY2240 including YAP6 at the t5 minute timepoint


title: SMY2237_WHI5_t15

source_name: SMY2237 including WHI5 at the t15 minute timepoint


title: SMY2152_GRX4_t5

source_name: SMY2152 including GRX4 at the t5 minute timepoint


title: SMY2152_GRX4_t0

source_name: SMY2152 including GRX4 at the t0 minute timepoint


title: SMY2152_GRX4_t10

source_name: SMY2152 including GRX4 at the t10 minute timepoint


title: SMY2152_GRX4_t15

source_name: SMY2152 including GRX4 at the t15 minute timepoint


title: SMY2151_GRX3_t45

source_name: SMY2151 including GRX3 at the t45 minute timepoint


title: SMY2152_GRX4_t20

source_name: SMY2152 including GRX4 at the t20 minute timepoint


title: SMY2151_GRX3_t30

source_name: SMY2151 including GRX3 at the t30 minute timepoint


title: SMY2151_GRX3_t20

source_name: SMY2151 including GRX3 at the t20 minute timepoint


title: SMY2151_GRX3_t90

source_name: SMY2151 including GRX3 at the t90 minute timepoint


title: SMY2151_GRX3_t15

source_name: SMY2151 including GRX3 at the t15 minute timepoint


title: SMY2152_GRX4_t45

source_name: SMY2152 including GRX4 at the t45 minute timepoint


title: SMY2151_GRX3_t10

source_name: SMY2151 including GRX3 at the t10 minute timepoint


title: SMY2151_GRX3_t0

source_name: SMY2151 including GRX3 at the t0 minute timepoint


title: SMY2151_GRX3_t5

source_name: SMY2151 including GRX3 at the t5 minute timepoint


title: SMY2110_RTG1_t90

source_name: SMY2110 including RTG1 at the t90 minute timepoint


title: SMY2110_RTG1_t30

source_name: SMY2110 including RTG1 at the t30 minute timepoint


title: SMY2110_RTG1_t20

source_name: SMY2110 including RTG1 at the t20 minute timepoint


title: SMY2110_RTG1_t45

source_name: SMY2110 including RTG1 at the t45 minute timepoint


title: SMY2152_GRX4_t30

source_name: SMY2152 including GRX4 at the t30 minute timepoint


title: SMY2110_RTG1_t5

source_name: SMY2110 including RTG1 at the t5 minute timepoint


title: SMY2104_RGT1_t90

source_name: SMY2104 including RGT1 at the t90 minute timepoint


title: SMY2110_RTG1_t15

source_name: SMY2110 including RTG1 at the t15 minute timepoint


title: SMY2110_RTG1_t10

source_name: SMY2110 including RTG1 at the t10 minute timepoint


title: SMY2104_RGT1_t5

source_name: SMY2104 including RGT1 at the t5 minute timepoint


title: SMY2104_RGT1_t0

source_name: SMY2104 including RGT1 at the t0 minute timepoint


title: SMY2104_RGT1_t20

source_name: SMY2104 including RGT1 at the t20 minute timepoint


title: SMY2118_STE12_t45

source_name: SMY2118 including STE12 at the t45 minute timepoint


title: SMY2104_RGT1_t10

source_name: SMY2104 including RGT1 at the t10 minute timepoint


title: SMY2104_RGT1_t45

source_name: SMY2104 including RGT1 at the t45 minute timepoint


title: SMY2118_STE12_t90

source_name: SMY2118 including STE12 at the t90 minute timepoint


title: SMY2110_RTG1_t0

source_name: SMY2110 including RTG1 at the t0 minute timepoint


title: SMY2104_RGT1_t30

source_name: SMY2104 including RGT1 at the t30 minute timepoint


title: SMY2118_STE12_t15

source_name: SMY2118 including STE12 at the t15 minute timepoint


title: SMY2118_STE12_t10

source_name: SMY2118 including STE12 at the t10 minute timepoint


title: SMY2118_STE12_t5

source_name: SMY2118 including STE12 at the t5 minute timepoint


title: SMY2104_RGT1_t15

source_name: SMY2104 including RGT1 at the t15 minute timepoint


title: SMY2118_STE12_t20

source_name: SMY2118 including STE12 at the t20 minute timepoint


title: SMY2224_STB1_t90

source_name: SMY2224 including STB1 at the t90 minute timepoint


title: SMY2224_STB1_t30

source_name: SMY2224 including STB1 at the t30 minute timepoint


title: SMY2118_STE12_t30

source_name: SMY2118 including STE12 at the t30 minute timepoint


title: SMY2224_STB1_t45

source_name: SMY2224 including STB1 at the t45 minute timepoint


title: SMY2224_STB1_t20

source_name: SMY2224 including STB1 at the t20 minute timepoint


title: SMY2225_SUB1_t15

source_name: SMY2225 including SUB1 at the t15 minute timepoint


title: SMY2225_SUB1_t10

source_name: SMY2225 including SUB1 at the t10 minute timepoint


title: SMY2225_SUB1_t30

source_name: SMY2225 including SUB1 at the t30 minute timepoint


title: SMY2225_SUB1_t0

source_name: SMY2225 including SUB1 at the t0 minute timepoint


title: SMY2225_SUB1_t5

source_name: SMY2225 including SUB1 at the t5 minute timepoint


title: SMY2225_SUB1_t20

source_name: SMY2225 including SUB1 at the t20 minute timepoint


title: SMY2224_STB1_t5

source_name: SMY2224 including STB1 at the t5 minute timepoint


title: SMY2224_STB1_t0

source_name: SMY2224 including STB1 at the t0 minute timepoint


title: SMY2224_STB1_t10

source_name: SMY2224 including STB1 at the t10 minute timepoint


title: SMY2223_SSN2_t30

source_name: SMY2223 including SSN2 at the t30 minute timepoint


title: SMY2223_SSN2_t90

source_name: SMY2223 including SSN2 at the t90 minute timepoint


title: SMY2223_SSN2_t20

source_name: SMY2223 including SSN2 at the t20 minute timepoint


title: SMY2224_STB1_t15

source_name: SMY2224 including STB1 at the t15 minute timepoint


title: SMY2225_SUB1_t45

source_name: SMY2225 including SUB1 at the t45 minute timepoint


title: SMY2223_SSN2_t15

source_name: SMY2223 including SSN2 at the t15 minute timepoint


title: SMY2222_SRB8_t90

source_name: SMY2222 including SRB8 at the t90 minute timepoint


title: SMY2223_SSN2_t0

source_name: SMY2223 including SSN2 at the t0 minute timepoint


title: SMY2223_SSN2_t10

source_name: SMY2223 including SSN2 at the t10 minute timepoint


title: SMY2223_SSN2_t45

source_name: SMY2223 including SSN2 at the t45 minute timepoint


title: SMY2223_SSN2_t5

source_name: SMY2223 including SSN2 at the t5 minute timepoint


title: SMY2222_SRB8_t45

source_name: SMY2222 including SRB8 at the t45 minute timepoint


title: SMY2222_SRB8_t30

source_name: SMY2222 including SRB8 at the t30 minute timepoint


title: SMY2222_SRB8_t10

source_name: SMY2222 including SRB8 at the t10 minute timepoint


title: SMY2222_SRB8_t15

source_name: SMY2222 including SRB8 at the t15 minute timepoint


title: SMY2069_GCR1_t5

source_name: SMY2069 including GCR1 at the t5 minute timepoint


title: SMY2222_SRB8_t5

source_name: SMY2222 including SRB8 at the t5 minute timepoint


title: SMY2222_SRB8_t0

source_name: SMY2222 including SRB8 at the t0 minute timepoint


title: SMY2220_SPT8_t20

source_name: SMY2220 including SPT8 at the t20 minute timepoint


title: SMY2221_SRB5_t30

source_name: SMY2221 including SRB5 at the t30 minute timepoint


title: SMY2221_SRB5_t10

source_name: SMY2221 including SRB5 at the t10 minute timepoint


title: SMY2221_SRB5_t15

source_name: SMY2221 including SRB5 at the t15 minute timepoint


title: SMY2221_SRB5_t5

source_name: SMY2221 including SRB5 at the t5 minute timepoint


title: SMY2220_SPT8_t45

source_name: SMY2220 including SPT8 at the t45 minute timepoint


title: SMY2220_SPT8_t90

source_name: SMY2220 including SPT8 at the t90 minute timepoint


title: SMY2221_SRB5_t20

source_name: SMY2221 including SRB5 at the t20 minute timepoint


title: SMY2220_SPT8_t10

source_name: SMY2220 including SPT8 at the t10 minute timepoint


title: SMY2222_SRB8_t20

source_name: SMY2222 including SRB8 at the t20 minute timepoint


title: SMY2225_SUB1_t90

source_name: SMY2225 including SUB1 at the t90 minute timepoint


title: SMY2118_STE12_t0

source_name: SMY2118 including STE12 at the t0 minute timepoint


title: SMY2219_SPT3_t30

source_name: SMY2219 including SPT3 at the t30 minute timepoint


title: SMY2219_SPT3_t20

source_name: SMY2219 including SPT3 at the t20 minute timepoint


title: SMY2219_SPT3_t45

source_name: SMY2219 including SPT3 at the t45 minute timepoint


title: SMY2220_SPT8_t0

source_name: SMY2220 including SPT8 at the t0 minute timepoint


title: SMY2219_SPT3_t15

source_name: SMY2219 including SPT3 at the t15 minute timepoint


title: SMY2219_SPT3_t0

source_name: SMY2219 including SPT3 at the t0 minute timepoint


title: SMY2221_SRB5_t0

source_name: SMY2221 including SRB5 at the t0 minute timepoint


title: SMY2219_SPT3_t5

source_name: SMY2219 including SPT3 at the t5 minute timepoint


title: SMY2219_SPT3_t10

source_name: SMY2219 including SPT3 at the t10 minute timepoint


title: SMY2217_SNF5_t90

source_name: SMY2217 including SNF5 at the t90 minute timepoint


title: SMY2217_SNF5_t30

source_name: SMY2217 including SNF5 at the t30 minute timepoint


title: SMY2221_SRB5_t90

source_name: SMY2221 including SRB5 at the t90 minute timepoint


title: SMY2220_SPT8_t5

source_name: SMY2220 including SPT8 at the t5 minute timepoint


title: SMY2219_SPT3_t90

source_name: SMY2219 including SPT3 at the t90 minute timepoint


title: SMY2263_SOK2_t5

source_name: SMY2263 including SOK2 at the t5 minute timepoint


title: SMY2217_SNF5_t15

source_name: SMY2217 including SNF5 at the t15 minute timepoint


title: SMY2217_SNF5_t10

source_name: SMY2217 including SNF5 at the t10 minute timepoint


title: SMY2217_SNF5_t5

source_name: SMY2217 including SNF5 at the t5 minute timepoint


title: SMY2216_SNF2_t45

source_name: SMY2216 including SNF2 at the t45 minute timepoint


title: SMY2217_SNF5_t0

source_name: SMY2217 including SNF5 at the t0 minute timepoint


title: SMY2216_SNF2_t90

source_name: SMY2216 including SNF2 at the t90 minute timepoint


title: SMY2216_SNF2_t30

source_name: SMY2216 including SNF2 at the t30 minute timepoint


title: SMY2216_SNF2_t15

source_name: SMY2216 including SNF2 at the t15 minute timepoint


title: SMY2216_SNF2_t20

source_name: SMY2216 including SNF2 at the t20 minute timepoint


title: SMY2217_SNF5_t20

source_name: SMY2217 including SNF5 at the t20 minute timepoint


title: SMY2216_SNF2_t10

source_name: SMY2216 including SNF2 at the t10 minute timepoint


title: SMY2215_SIP3_t45

source_name: SMY2215 including SIP3 at the t45 minute timepoint


title: SMY2216_SNF2_t5

source_name: SMY2216 including SNF2 at the t5 minute timepoint


title: SMY2215_SIP3_t90

source_name: SMY2215 including SIP3 at the t90 minute timepoint


title: SMY2215_SIP3_t20

source_name: SMY2215 including SIP3 at the t20 minute timepoint


title: SMY2214_SIN3_t30

source_name: SMY2214 including SIN3 at the t30 minute timepoint


title: SMY2215_SIP3_t15

source_name: SMY2215 including SIP3 at the t15 minute timepoint


title: SMY2215_SIP3_t30

source_name: SMY2215 including SIP3 at the t30 minute timepoint


title: SMY2214_SIN3_t90

source_name: SMY2214 including SIN3 at the t90 minute timepoint


title: SMY2215_SIP3_t10

source_name: SMY2215 including SIP3 at the t10 minute timepoint


title: SMY2214_SIN3_t45

source_name: SMY2214 including SIN3 at the t45 minute timepoint


title: SMY2215_SIP3_t0

source_name: SMY2215 including SIP3 at the t0 minute timepoint


title: SMY2214_SIN3_t20

source_name: SMY2214 including SIN3 at the t20 minute timepoint


title: SMY2214_SIN3_t10

source_name: SMY2214 including SIN3 at the t10 minute timepoint


title: SMY2216_SNF2_t0

source_name: SMY2216 including SNF2 at the t0 minute timepoint


title: SMY2214_SIN3_t15

source_name: SMY2214 including SIN3 at the t15 minute timepoint


title: SMY2214_SIN3_t5

source_name: SMY2214 including SIN3 at the t5 minute timepoint


title: SMY2220_SPT8_t30

source_name: SMY2220 including SPT8 at the t30 minute timepoint


title: SMY2214_SIN3_t0

source_name: SMY2214 including SIN3 at the t0 minute timepoint


title: SMY2236_VHR1_t10

source_name: SMY2236 including VHR1 at the t10 minute timepoint


title: SMY2236_VHR1_t90

source_name: SMY2236 including VHR1 at the t90 minute timepoint


title: SMY2236_VHR1_t45

source_name: SMY2236 including VHR1 at the t45 minute timepoint


title: SMY2236_VHR1_t15

source_name: SMY2236 including VHR1 at the t15 minute timepoint


title: SMY2236_VHR1_t30

source_name: SMY2236 including VHR1 at the t30 minute timepoint


title: SMY2215_SIP3_t5

source_name: SMY2215 including SIP3 at the t5 minute timepoint


title: SMY2236_VHR1_t0

source_name: SMY2236 including VHR1 at the t0 minute timepoint


title: SMY2235_URE2_t90

source_name: SMY2235 including URE2 at the t90 minute timepoint


title: SMY2234_UME1_t45

source_name: SMY2234 including UME1 at the t45 minute timepoint


title: SMY2235_URE2_t45

source_name: SMY2235 including URE2 at the t45 minute timepoint


title: SMY2236_VHR1_t5

source_name: SMY2236 including VHR1 at the t5 minute timepoint


title: SMY2234_UME1_t30

source_name: SMY2234 including UME1 at the t30 minute timepoint


title: SMY2234_UME1_t90

source_name: SMY2234 including UME1 at the t90 minute timepoint


title: SMY2235_URE2_t15

source_name: SMY2235 including URE2 at the t15 minute timepoint


title: SMY2234_UME1_t20

source_name: SMY2234 including UME1 at the t20 minute timepoint


title: SMY2235_URE2_t0

source_name: SMY2235 including URE2 at the t0 minute timepoint


title: SMY2235_URE2_t5

source_name: SMY2235 including URE2 at the t5 minute timepoint


title: SMY2235_URE2_t10

source_name: SMY2235 including URE2 at the t10 minute timepoint


title: SMY2235_URE2_t30

source_name: SMY2235 including URE2 at the t30 minute timepoint


title: SMY2235_URE2_t20

source_name: SMY2235 including URE2 at the t20 minute timepoint


title: SMY2234_UME1_t15

source_name: SMY2234 including UME1 at the t15 minute timepoint


title: SMY2234_UME1_t10

source_name: SMY2234 including UME1 at the t10 minute timepoint


title: SMY2234_UME1_t5

source_name: SMY2234 including UME1 at the t5 minute timepoint


title: SMY2234_UME1_t0

source_name: SMY2234 including UME1 at the t0 minute timepoint


title: SMY2236_VHR1_t20

source_name: SMY2236 including VHR1 at the t20 minute timepoint


title: SMY2233_UAF30_t90

source_name: SMY2233 including UAF30 at the t90 minute timepoint


title: SMY2233_UAF30_t20

source_name: SMY2233 including UAF30 at the t20 minute timepoint


title: SMY2233_UAF30_t15

source_name: SMY2233 including UAF30 at the t15 minute timepoint


title: SMY2233_UAF30_t45

source_name: SMY2233 including UAF30 at the t45 minute timepoint


title: SMY2233_UAF30_t10

source_name: SMY2233 including UAF30 at the t10 minute timepoint


title: SMY2233_UAF30_t5

source_name: SMY2233 including UAF30 at the t5 minute timepoint


title: SMY2233_UAF30_t30

source_name: SMY2233 including UAF30 at the t30 minute timepoint


title: SMY2233_UAF30_t0

source_name: SMY2233 including UAF30 at the t0 minute timepoint


title: SMY2232_TYE7_t45

source_name: SMY2232 including TYE7 at the t45 minute timepoint


title: SMY2232_TYE7_t90

source_name: SMY2232 including TYE7 at the t90 minute timepoint


title: SMY2220_SPT8_t15

source_name: SMY2220 including SPT8 at the t15 minute timepoint


title: SMY2232_TYE7_t5

source_name: SMY2232 including TYE7 at the t5 minute timepoint


title: SMY2232_TYE7_t10

source_name: SMY2232 including TYE7 at the t10 minute timepoint


title: SMY2230_TOG1_t90

source_name: SMY2230 including TOG1 at the t90 minute timepoint


title: SMY2230_TOG1_t30

source_name: SMY2230 including TOG1 at the t30 minute timepoint


title: SMY2230_TOG1_t45

source_name: SMY2230 including TOG1 at the t45 minute timepoint


title: SMY2232_TYE7_t0

source_name: SMY2232 including TYE7 at the t0 minute timepoint


title: SMY2232_TYE7_t30

source_name: SMY2232 including TYE7 at the t30 minute timepoint


title: SMY2230_TOG1_t10

source_name: SMY2230 including TOG1 at the t10 minute timepoint


title: SMY2230_TOG1_t15

source_name: SMY2230 including TOG1 at the t15 minute timepoint


title: SMY2230_TOG1_t5

source_name: SMY2230 including TOG1 at the t5 minute timepoint


title: SMY2230_TOG1_t0

source_name: SMY2230 including TOG1 at the t0 minute timepoint


title: SMY2230_TOG1_t20

source_name: SMY2230 including TOG1 at the t20 minute timepoint


title: SMY2232_TYE7_t15

source_name: SMY2232 including TYE7 at the t15 minute timepoint


title: SMY2229_THI3_t90

source_name: SMY2229 including THI3 at the t90 minute timepoint


title: SMY2229_THI3_t45

source_name: SMY2229 including THI3 at the t45 minute timepoint


title: SMY2232_TYE7_t20

source_name: SMY2232 including TYE7 at the t20 minute timepoint


title: SMY2229_THI3_t30

source_name: SMY2229 including THI3 at the t30 minute timepoint


title: SMY2229_THI3_t20

source_name: SMY2229 including THI3 at the t20 minute timepoint


title: SMY2229_THI3_t15

source_name: SMY2229 including THI3 at the t15 minute timepoint


title: SMY2229_THI3_t5

source_name: SMY2229 including THI3 at the t5 minute timepoint


title: SMY2228_TFB5_t30

source_name: SMY2228 including TFB5 at the t30 minute timepoint


title: SMY2229_THI3_t10

source_name: SMY2229 including THI3 at the t10 minute timepoint


title: SMY2228_TFB5_t45

source_name: SMY2228 including TFB5 at the t45 minute timepoint


title: SMY2228_TFB5_t20

source_name: SMY2228 including TFB5 at the t20 minute timepoint


title: SMY2228_TFB5_t15

source_name: SMY2228 including TFB5 at the t15 minute timepoint


title: SMY2228_TFB5_t10

source_name: SMY2228 including TFB5 at the t10 minute timepoint


title: SMY2226_SWI6_t45

source_name: SMY2226 including SWI6 at the t45 minute timepoint


title: SMY2226_SWI6_t20

source_name: SMY2226 including SWI6 at the t20 minute timepoint


title: SMY2227_TAF2_t90

source_name: SMY2227 including TAF2 at the t90 minute timepoint


title: SMY2228_TFB5_t90

source_name: SMY2228 including TFB5 at the t90 minute timepoint


title: SMY2226_SWI6_t30

source_name: SMY2226 including SWI6 at the t30 minute timepoint


title: SMY2228_TFB5_t5

source_name: SMY2228 including TFB5 at the t5 minute timepoint


title: SMY2229_THI3_t0

source_name: SMY2229 including THI3 at the t0 minute timepoint


title: SMY2227_TAF2_t15

source_name: SMY2227 including TAF2 at the t15 minute timepoint


title: SMY2226_SWI6_t90

source_name: SMY2226 including SWI6 at the t90 minute timepoint


title: SMY2227_TAF2_t0

source_name: SMY2227 including TAF2 at the t0 minute timepoint


title: SMY2227_TAF2_t5

source_name: SMY2227 including TAF2 at the t5 minute timepoint


title: SMY2226_SWI6_t15

source_name: SMY2226 including SWI6 at the t15 minute timepoint


title: SMY2227_TAF2_t20

source_name: SMY2227 including TAF2 at the t20 minute timepoint


title: SMY2227_TAF2_t30

source_name: SMY2227 including TAF2 at the t30 minute timepoint


title: SMY2228_TFB5_t0

source_name: SMY2228 including TFB5 at the t0 minute timepoint


title: SMY2226_SWI6_t5

source_name: SMY2226 including SWI6 at the t5 minute timepoint


title: SMY2206_RDR1_t45

source_name: SMY2206 including RDR1 at the t45 minute timepoint


title: SMY2206_RDR1_t90

source_name: SMY2206 including RDR1 at the t90 minute timepoint


title: SMY2226_SWI6_t0

source_name: SMY2226 including SWI6 at the t0 minute timepoint


title: SMY2206_RDR1_t15

source_name: SMY2206 including RDR1 at the t15 minute timepoint


title: SMY2206_RDR1_t10

source_name: SMY2206 including RDR1 at the t10 minute timepoint


title: SMY2226_SWI6_t10

source_name: SMY2226 including SWI6 at the t10 minute timepoint


title: SMY2206_RDR1_t5

source_name: SMY2206 including RDR1 at the t5 minute timepoint


title: SMY2206_RDR1_t0

source_name: SMY2206 including RDR1 at the t0 minute timepoint


title: SMY2206_RDR1_t30

source_name: SMY2206 including RDR1 at the t30 minute timepoint


title: SMY2197_LEO1_t30

source_name: SMY2197 including LEO1 at the t30 minute timepoint


title: SMY2197_LEO1_t45

source_name: SMY2197 including LEO1 at the t45 minute timepoint


title: SMY2197_LEO1_t20

source_name: SMY2197 including LEO1 at the t20 minute timepoint


title: SMY2197_LEO1_t15

source_name: SMY2197 including LEO1 at the t15 minute timepoint


title: SMY2197_LEO1_t5

source_name: SMY2197 including LEO1 at the t5 minute timepoint


title: SMY2197_LEO1_t10

source_name: SMY2197 including LEO1 at the t10 minute timepoint


title: SMY2197_LEO1_t0

source_name: SMY2197 including LEO1 at the t0 minute timepoint


title: SMY2204_PGD1_t90

source_name: SMY2204 including PGD1 at the t90 minute timepoint


title: SMY2204_PGD1_t10

source_name: SMY2204 including PGD1 at the t10 minute timepoint


title: SMY2204_PGD1_t20

source_name: SMY2204 including PGD1 at the t20 minute timepoint


title: SMY2204_PGD1_t5

source_name: SMY2204 including PGD1 at the t5 minute timepoint


title: SMY2204_PGD1_t30

source_name: SMY2204 including PGD1 at the t30 minute timepoint


title: SMY2100_PIP2_t90

source_name: SMY2100 including PIP2 at the t90 minute timepoint


title: SMY2100_PIP2_t10

source_name: SMY2100 including PIP2 at the t10 minute timepoint


title: SMY2100_PIP2_t15

source_name: SMY2100 including PIP2 at the t15 minute timepoint


title: SMY2100_PIP2_t45

source_name: SMY2100 including PIP2 at the t45 minute timepoint


title: SMY2096_PDR1_t45

source_name: SMY2096 including PDR1 at the t45 minute timepoint


title: SMY2204_PGD1_t45

source_name: SMY2204 including PGD1 at the t45 minute timepoint


title: SMY2204_PGD1_t0

source_name: SMY2204 including PGD1 at the t0 minute timepoint


title: SMY2100_PIP2_t5

source_name: SMY2100 including PIP2 at the t5 minute timepoint


title: SMY2096_PDR1_t30

source_name: SMY2096 including PDR1 at the t30 minute timepoint


title: SMY2096_PDR1_t90

source_name: SMY2096 including PDR1 at the t90 minute timepoint


title: SMY2204_PGD1_t15

source_name: SMY2204 including PGD1 at the t15 minute timepoint


title: SMY2227_TAF2_t10

source_name: SMY2227 including TAF2 at the t10 minute timepoint


title: SMY2100_PIP2_t0

source_name: SMY2100 including PIP2 at the t0 minute timepoint


title: SMY2096_PDR1_t20

source_name: SMY2096 including PDR1 at the t20 minute timepoint


title: SMY2096_PDR1_t10

source_name: SMY2096 including PDR1 at the t10 minute timepoint


title: SMY2096_PDR1_t5

source_name: SMY2096 including PDR1 at the t5 minute timepoint


title: SMY2096_PDR1_t15

source_name: SMY2096 including PDR1 at the t15 minute timepoint


title: SMY2094_NRG1_t15

source_name: SMY2094 including NRG1 at the t15 minute timepoint


title: SMY2094_NRG1_t20

source_name: SMY2094 including NRG1 at the t20 minute timepoint


title: SMY2094_NRG1_t10

source_name: SMY2094 including NRG1 at the t10 minute timepoint


title: SMY2094_NRG1_t30

source_name: SMY2094 including NRG1 at the t30 minute timepoint


title: SMY2094_NRG1_t45

source_name: SMY2094 including NRG1 at the t45 minute timepoint


title: SMY2094_NRG1_t0

source_name: SMY2094 including NRG1 at the t0 minute timepoint


title: SMY2092_MSS11_t90

source_name: SMY2092 including MSS11 at the t90 minute timepoint


title: SMY2094_NRG1_t90

source_name: SMY2094 including NRG1 at the t90 minute timepoint


title: SMY2096_PDR1_t0

source_name: SMY2096 including PDR1 at the t0 minute timepoint


title: SMY2094_NRG1_t5

source_name: SMY2094 including NRG1 at the t5 minute timepoint


title: SMY2092_MSS11_t45

source_name: SMY2092 including MSS11 at the t45 minute timepoint


title: SMY2088_MIG3_t90

source_name: SMY2088 including MIG3 at the t90 minute timepoint


title: SMY2092_MSS11_t20

source_name: SMY2092 including MSS11 at the t20 minute timepoint


title: SMY2092_MSS11_t10

source_name: SMY2092 including MSS11 at the t10 minute timepoint


title: SMY2092_MSS11_t0

source_name: SMY2092 including MSS11 at the t0 minute timepoint


title: SMY2092_MSS11_t5

source_name: SMY2092 including MSS11 at the t5 minute timepoint


title: SMY2088_MIG3_t45

source_name: SMY2088 including MIG3 at the t45 minute timepoint


title: SMY2092_MSS11_t15

source_name: SMY2092 including MSS11 at the t15 minute timepoint


title: SMY2088_MIG3_t10

source_name: SMY2088 including MIG3 at the t10 minute timepoint


title: SMY2088_MIG3_t15

source_name: SMY2088 including MIG3 at the t15 minute timepoint


title: SMY2088_MIG3_t30

source_name: SMY2088 including MIG3 at the t30 minute timepoint


title: SMY2088_MIG3_t20

source_name: SMY2088 including MIG3 at the t20 minute timepoint


title: SMY2092_MSS11_t30

source_name: SMY2092 including MSS11 at the t30 minute timepoint


title: SMY2197_LEO1_t90

source_name: SMY2197 including LEO1 at the t90 minute timepoint


title: SMY2061_CHA4_t90

source_name: SMY2061 including CHA4 at the t90 minute timepoint


title: SMY2088_MIG3_t5

source_name: SMY2088 including MIG3 at the t5 minute timepoint


title: SMY2061_CHA4_t30

source_name: SMY2061 including CHA4 at the t30 minute timepoint


title: SMY2206_RDR1_t20

source_name: SMY2206 including RDR1 at the t20 minute timepoint


title: SMY2060_CAT8_t90

source_name: SMY2060 including CAT8 at the t90 minute timepoint


title: SMY2088_MIG3_t0

source_name: SMY2088 including MIG3 at the t0 minute timepoint


title: SMY2060_CAT8_t30

source_name: SMY2060 including CAT8 at the t30 minute timepoint


title: SMY2061_CHA4_t5

source_name: SMY2061 including CHA4 at the t5 minute timepoint


title: SMY2061_CHA4_t20

source_name: SMY2061 including CHA4 at the t20 minute timepoint


title: SMY2060_CAT8_t20

source_name: SMY2060 including CAT8 at the t20 minute timepoint


title: SMY2060_CAT8_t45

source_name: SMY2060 including CAT8 at the t45 minute timepoint


title: SMY2058_BAS1_t30

source_name: SMY2058 including BAS1 at the t30 minute timepoint


title: SMY2061_CHA4_t45

source_name: SMY2061 including CHA4 at the t45 minute timepoint


title: SMY2058_BAS1_t45

source_name: SMY2058 including BAS1 at the t45 minute timepoint


title: SMY2061_CHA4_t0

source_name: SMY2061 including CHA4 at the t0 minute timepoint


title: SMY2060_CAT8_t10

source_name: SMY2060 including CAT8 at the t10 minute timepoint


title: SMY2061_CHA4_t15

source_name: SMY2061 including CHA4 at the t15 minute timepoint


title: SMY2058_BAS1_t10

source_name: SMY2058 including BAS1 at the t10 minute timepoint


title: SMY2058_BAS1_t20

source_name: SMY2058 including BAS1 at the t20 minute timepoint


title: SMY2058_BAS1_t0

source_name: SMY2058 including BAS1 at the t0 minute timepoint


title: SMY2058_BAS1_t5

source_name: SMY2058 including BAS1 at the t5 minute timepoint


title: SMY2060_CAT8_t5

source_name: SMY2060 including CAT8 at the t5 minute timepoint


title: SMY2060_CAT8_t15

source_name: SMY2060 including CAT8 at the t15 minute timepoint


title: SMY2052_AFT2_t45

source_name: SMY2052 including AFT2 at the t45 minute timepoint


title: SMY2060_CAT8_t0

source_name: SMY2060 including CAT8 at the t0 minute timepoint


title: SMY2213_SFP1_t90

source_name: SMY2213 including SFP1 at the t90 minute timepoint


title: SMY2052_AFT2_t30

source_name: SMY2052 including AFT2 at the t30 minute timepoint


title: SMY2052_AFT2_t90

source_name: SMY2052 including AFT2 at the t90 minute timepoint


title: SMY2052_AFT2_t15

source_name: SMY2052 including AFT2 at the t15 minute timepoint


title: SMY2213_SFP1_t15

source_name: SMY2213 including SFP1 at the t15 minute timepoint


title: SMY2052_AFT2_t10

source_name: SMY2052 including AFT2 at the t10 minute timepoint


title: SMY2213_SFP1_t30

source_name: SMY2213 including SFP1 at the t30 minute timepoint


title: SMY2213_SFP1_t20

source_name: SMY2213 including SFP1 at the t20 minute timepoint


title: SMY2213_SFP1_t10

source_name: SMY2213 including SFP1 at the t10 minute timepoint


title: SMY2213_SFP1_t0

source_name: SMY2213 including SFP1 at the t0 minute timepoint


title: SMY2052_AFT2_t20

source_name: SMY2052 including AFT2 at the t20 minute timepoint


title: SMY2213_SFP1_t45

source_name: SMY2213 including SFP1 at the t45 minute timepoint


title: SMY2212_RSF2_t90

source_name: SMY2212 including RSF2 at the t90 minute timepoint


title: SMY2052_AFT2_t0

source_name: SMY2052 including AFT2 at the t0 minute timepoint


title: SMY2052_AFT2_t5

source_name: SMY2052 including AFT2 at the t5 minute timepoint


title: SMY2212_RSF2_t0

source_name: SMY2212 including RSF2 at the t0 minute timepoint


title: SMY2212_RSF2_t15

source_name: SMY2212 including RSF2 at the t15 minute timepoint


title: SMY2212_RSF2_t45

source_name: SMY2212 including RSF2 at the t45 minute timepoint


title: SMY2212_RSF2_t10

source_name: SMY2212 including RSF2 at the t10 minute timepoint


title: SMY2211_RRN10_t45

source_name: SMY2211 including RRN10 at the t45 minute timepoint


title: SMY2211_RRN10_t90

source_name: SMY2211 including RRN10 at the t90 minute timepoint


title: SMY2100_PIP2_t30

source_name: SMY2100 including PIP2 at the t30 minute timepoint


title: SMY2213_SFP1_t5

source_name: SMY2213 including SFP1 at the t5 minute timepoint


title: SMY2211_RRN10_t20

source_name: SMY2211 including RRN10 at the t20 minute timepoint


title: SMY2211_RRN10_t30

source_name: SMY2211 including RRN10 at the t30 minute timepoint


title: SMY2211_RRN10_t0

source_name: SMY2211 including RRN10 at the t0 minute timepoint


title: SMY2210_RIM101_t30

source_name: SMY2210 including RIM101 at the t30 minute timepoint


title: SMY2211_RRN10_t15

source_name: SMY2211 including RRN10 at the t15 minute timepoint


title: SMY2211_RRN10_t5

source_name: SMY2211 including RRN10 at the t5 minute timepoint


title: SMY2210_RIM101_t90

source_name: SMY2210 including RIM101 at the t90 minute timepoint


title: SMY2210_RIM101_t45

source_name: SMY2210 including RIM101 at the t45 minute timepoint


title: SMY2210_RIM101_t20

source_name: SMY2210 including RIM101 at the t20 minute timepoint


title: SMY2210_RIM101_t15

source_name: SMY2210 including RIM101 at the t15 minute timepoint


title: SMY2209_RGR1_t30

source_name: SMY2209 including RGR1 at the t30 minute timepoint


title: SMY2209_RGR1_t45

source_name: SMY2209 including RGR1 at the t45 minute timepoint


title: SMY2209_RGR1_t15

source_name: SMY2209 including RGR1 at the t15 minute timepoint


title: SMY2209_RGR1_t10

source_name: SMY2209 including RGR1 at the t10 minute timepoint


title: SMY2210_RIM101_t0

source_name: SMY2210 including RIM101 at the t0 minute timepoint


title: SMY2210_RIM101_t5

source_name: SMY2210 including RIM101 at the t5 minute timepoint


title: SMY2209_RGR1_t5

source_name: SMY2209 including RGR1 at the t5 minute timepoint


title: SMY2209_RGR1_t20

source_name: SMY2209 including RGR1 at the t20 minute timepoint


title: SMY2210_RIM101_t10

source_name: SMY2210 including RIM101 at the t10 minute timepoint


title: SMY2208_RGM1_t45

source_name: SMY2208 including RGM1 at the t45 minute timepoint


title: SMY2211_RRN10_t10

source_name: SMY2211 including RRN10 at the t10 minute timepoint


title: SMY2208_RGM1_t90

source_name: SMY2208 including RGM1 at the t90 minute timepoint


title: SMY2209_RGR1_t0

source_name: SMY2209 including RGR1 at the t0 minute timepoint


title: SMY2209_RGR1_t90

source_name: SMY2209 including RGR1 at the t90 minute timepoint


title: SMY2208_RGM1_t30

source_name: SMY2208 including RGM1 at the t30 minute timepoint


title: SMY2208_RGM1_t20

source_name: SMY2208 including RGM1 at the t20 minute timepoint


title: SMY2212_RSF2_t5

source_name: SMY2212 including RSF2 at the t5 minute timepoint


title: SMY2208_RGM1_t15

source_name: SMY2208 including RGM1 at the t15 minute timepoint


title: SMY2208_RGM1_t5

source_name: SMY2208 including RGM1 at the t5 minute timepoint


title: SMY2205_PHO2_t15

source_name: SMY2205 including PHO2 at the t15 minute timepoint


title: SMY2205_PHO2_t20

source_name: SMY2205 including PHO2 at the t20 minute timepoint


title: SMY2192_HFI1_t90

source_name: SMY2192 including HFI1 at the t90 minute timepoint


title: SMY2205_PHO2_t90

source_name: SMY2205 including PHO2 at the t90 minute timepoint


title: SMY2192_HFI1_t45

source_name: SMY2192 including HFI1 at the t45 minute timepoint


title: SMY2208_RGM1_t0

source_name: SMY2208 including RGM1 at the t0 minute timepoint


title: SMY2208_RGM1_t10

source_name: SMY2208 including RGM1 at the t10 minute timepoint


title: SMY2205_PHO2_t30

source_name: SMY2205 including PHO2 at the t30 minute timepoint


title: SMY2205_PHO2_t10

source_name: SMY2205 including PHO2 at the t10 minute timepoint


title: SMY2061_CHA4_t10

source_name: SMY2061 including CHA4 at the t10 minute timepoint


title: SMY2205_PHO2_t0

source_name: SMY2205 including PHO2 at the t0 minute timepoint


title: SMY2205_PHO2_t5

source_name: SMY2205 including PHO2 at the t5 minute timepoint


title: SMY2192_HFI1_t5

source_name: SMY2192 including HFI1 at the t5 minute timepoint


title: SMY2205_PHO2_t45

source_name: SMY2205 including PHO2 at the t45 minute timepoint


title: SMY2192_HFI1_t20

source_name: SMY2192 including HFI1 at the t20 minute timepoint


title: SMY2192_HFI1_t15

source_name: SMY2192 including HFI1 at the t15 minute timepoint


title: SMY2192_HFI1_t30

source_name: SMY2192 including HFI1 at the t30 minute timepoint


title: SMY2192_HFI1_t10

source_name: SMY2192 including HFI1 at the t10 minute timepoint


title: SMY2173_PAF1_t45

source_name: SMY2173 including PAF1 at the t45 minute timepoint


title: SMY2173_PAF1_t90

source_name: SMY2173 including PAF1 at the t90 minute timepoint


title: SMY2173_PAF1_t15

source_name: SMY2173 including PAF1 at the t15 minute timepoint


title: SMY2173_PAF1_t30

source_name: SMY2173 including PAF1 at the t30 minute timepoint


title: SMY2212_RSF2_t20

source_name: SMY2212 including RSF2 at the t20 minute timepoint


title: SMY2173_PAF1_t20

source_name: SMY2173 including PAF1 at the t20 minute timepoint


title: SMY2173_PAF1_t0

source_name: SMY2173 including PAF1 at the t0 minute timepoint


title: SMY2173_PAF1_t10

source_name: SMY2173 including PAF1 at the t10 minute timepoint


title: SMY2173_PAF1_t5

source_name: SMY2173 including PAF1 at the t5 minute timepoint


title: SMY2192_HFI1_t0

source_name: SMY2192 including HFI1 at the t0 minute timepoint


title: SMY2203_NRM1_t90

source_name: SMY2203 including NRM1 at the t90 minute timepoint


title: SMY2203_NRM1_t5

source_name: SMY2203 including NRM1 at the t5 minute timepoint


title: SMY2203_NRM1_t0

source_name: SMY2203 including NRM1 at the t0 minute timepoint


title: SMY2202_MTF1_t10

source_name: SMY2202 including MTF1 at the t10 minute timepoint


title: SMY2203_NRM1_t30

source_name: SMY2203 including NRM1 at the t30 minute timepoint


title: SMY2202_MTF1_t90

source_name: SMY2202 including MTF1 at the t90 minute timepoint


title: SMY2203_NRM1_t10

source_name: SMY2203 including NRM1 at the t10 minute timepoint


title: SMY2203_NRM1_t20

source_name: SMY2203 including NRM1 at the t20 minute timepoint


title: SMY2203_NRM1_t15

source_name: SMY2203 including NRM1 at the t15 minute timepoint


title: SMY2196_KSS1_t45

source_name: SMY2196 including KSS1 at the t45 minute timepoint


title: SMY2196_KSS1_t90

source_name: SMY2196 including KSS1 at the t90 minute timepoint


title: SMY2196_KSS1_t20

source_name: SMY2196 including KSS1 at the t20 minute timepoint


title: SMY2202_MTF1_t0

source_name: SMY2202 including MTF1 at the t0 minute timepoint


title: SMY2196_KSS1_t30

source_name: SMY2196 including KSS1 at the t30 minute timepoint


title: SMY2202_MTF1_t20

source_name: SMY2202 including MTF1 at the t20 minute timepoint


title: SMY2196_KSS1_t10

source_name: SMY2196 including KSS1 at the t10 minute timepoint


title: SMY2196_KSS1_t15

source_name: SMY2196 including KSS1 at the t15 minute timepoint


title: SMY2202_MTF1_t30

source_name: SMY2202 including MTF1 at the t30 minute timepoint


title: SMY2203_NRM1_t45

source_name: SMY2203 including NRM1 at the t45 minute timepoint


title: SMY2195_HMS1_t90

source_name: SMY2195 including HMS1 at the t90 minute timepoint


title: SMY2196_KSS1_t5

source_name: SMY2196 including KSS1 at the t5 minute timepoint


title: SMY2195_HMS1_t45

source_name: SMY2195 including HMS1 at the t45 minute timepoint


title: SMY2196_KSS1_t0

source_name: SMY2196 including KSS1 at the t0 minute timepoint


title: SMY2202_MTF1_t5

source_name: SMY2202 including MTF1 at the t5 minute timepoint


title: SMY2195_HMS1_t30

source_name: SMY2195 including HMS1 at the t30 minute timepoint


title: SMY2195_HMS1_t15

source_name: SMY2195 including HMS1 at the t15 minute timepoint


title: SMY2202_MTF1_t45

source_name: SMY2202 including MTF1 at the t45 minute timepoint


title: SMY2202_MTF1_t15

source_name: SMY2202 including MTF1 at the t15 minute timepoint


title: SMY2195_HMS1_t20

source_name: SMY2195 including HMS1 at the t20 minute timepoint


title: SMY2195_HMS1_t10

source_name: SMY2195 including HMS1 at the t10 minute timepoint


title: SMY2194_HIR3_t15

source_name: SMY2194 including HIR3 at the t15 minute timepoint


title: SMY2194_HIR3_t45

source_name: SMY2194 including HIR3 at the t45 minute timepoint


title: SMY2194_HIR3_t90

source_name: SMY2194 including HIR3 at the t90 minute timepoint


title: SMY2194_HIR3_t20

source_name: SMY2194 including HIR3 at the t20 minute timepoint


title: SMY2194_HIR3_t30

source_name: SMY2194 including HIR3 at the t30 minute timepoint


title: SMY2194_HIR3_t5

source_name: SMY2194 including HIR3 at the t5 minute timepoint


title: SMY2195_HMS1_t0

source_name: SMY2195 including HMS1 at the t0 minute timepoint


title: SMY2193_HIR1_t10

source_name: SMY2193 including HIR1 at the t10 minute timepoint


title: SMY2193_HIR1_t20

source_name: SMY2193 including HIR1 at the t20 minute timepoint


title: SMY2193_HIR1_t15

source_name: SMY2193 including HIR1 at the t15 minute timepoint


title: SMY2193_HIR1_t5

source_name: SMY2193 including HIR1 at the t5 minute timepoint


title: SMY2194_HIR3_t10

source_name: SMY2194 including HIR3 at the t10 minute timepoint


title: SMY2191_GTS1_t15

source_name: SMY2191 including GTS1 at the t15 minute timepoint


title: SMY2193_HIR1_t0

source_name: SMY2193 including HIR1 at the t0 minute timepoint


title: SMY2193_HIR1_t30

source_name: SMY2193 including HIR1 at the t30 minute timepoint


title: SMY2193_HIR1_t90

source_name: SMY2193 including HIR1 at the t90 minute timepoint


title: SMY2191_GTS1_t30

source_name: SMY2191 including GTS1 at the t30 minute timepoint


title: SMY2193_HIR1_t45

source_name: SMY2193 including HIR1 at the t45 minute timepoint


title: SMY2191_GTS1_t90

source_name: SMY2191 including GTS1 at the t90 minute timepoint


title: SMY2191_GTS1_t45

source_name: SMY2191 including GTS1 at the t45 minute timepoint


title: SMY2191_GTS1_t10

source_name: SMY2191 including GTS1 at the t10 minute timepoint


title: SMY2194_HIR3_t0

source_name: SMY2194 including HIR3 at the t0 minute timepoint


title: SMY2195_HMS1_t5

source_name: SMY2195 including HMS1 at the t5 minute timepoint


title: SMY2191_GTS1_t5

source_name: SMY2191 including GTS1 at the t5 minute timepoint


title: SMY2190_GCN5_t90

source_name: SMY2190 including GCN5 at the t90 minute timepoint


title: SMY2217_SNF5_t45

source_name: SMY2217 including SNF5 at the t45 minute timepoint


title: SMY2191_GTS1_t0

source_name: SMY2191 including GTS1 at the t0 minute timepoint


title: SMY2190_GCN5_t45

source_name: SMY2190 including GCN5 at the t45 minute timepoint


title: SMY2191_GTS1_t20

source_name: SMY2191 including GTS1 at the t20 minute timepoint


title: SMY2190_GCN5_t30

source_name: SMY2190 including GCN5 at the t30 minute timepoint


title: SMY2190_GCN5_t15

source_name: SMY2190 including GCN5 at the t15 minute timepoint


title: SMY2189_GAT2_t45

source_name: SMY2189 including GAT2 at the t45 minute timepoint


title: SMY2190_GCN5_t5

source_name: SMY2190 including GCN5 at the t5 minute timepoint


title: SMY2189_GAT2_t20

source_name: SMY2189 including GAT2 at the t20 minute timepoint


title: SMY2189_GAT2_t90

source_name: SMY2189 including GAT2 at the t90 minute timepoint


title: SMY2189_GAT2_t30

source_name: SMY2189 including GAT2 at the t30 minute timepoint


title: SMY2190_GCN5_t20

source_name: SMY2190 including GCN5 at the t20 minute timepoint


title: SMY2109_RPN4_t15

source_name: SMY2109 including RPN4 at the t15 minute timepoint


title: SMY2189_GAT2_t15

source_name: SMY2189 including GAT2 at the t15 minute timepoint


title: SMY2109_RPN4_t45

source_name: SMY2109 including RPN4 at the t45 minute timepoint


title: SMY2109_RPN4_t20

source_name: SMY2109 including RPN4 at the t20 minute timepoint


title: SMY2109_RPN4_t30

source_name: SMY2109 including RPN4 at the t30 minute timepoint


title: SMY2109_RPN4_t90

source_name: SMY2109 including RPN4 at the t90 minute timepoint


title: SMY2189_GAT2_t5

source_name: SMY2189 including GAT2 at the t5 minute timepoint


title: SMY2189_GAT2_t0

source_name: SMY2189 including GAT2 at the t0 minute timepoint


title: SMY2190_GCN5_t10

source_name: SMY2190 including GCN5 at the t10 minute timepoint


title: SMY2109_RPN4_t10

source_name: SMY2109 including RPN4 at the t10 minute timepoint


title: SMY2109_RPN4_t5

source_name: SMY2109 including RPN4 at the t5 minute timepoint


title: SMY2187_FAP1_t90

source_name: SMY2187 including FAP1 at the t90 minute timepoint


title: SMY2189_GAT2_t10

source_name: SMY2189 including GAT2 at the t10 minute timepoint


title: SMY2109_RPN4_t0

source_name: SMY2109 including RPN4 at the t0 minute timepoint


title: SMY2190_GCN5_t0

source_name: SMY2190 including GCN5 at the t0 minute timepoint


title: SMY2187_FAP1_t45

source_name: SMY2187 including FAP1 at the t45 minute timepoint


title: SMY2187_FAP1_t0

source_name: SMY2187 including FAP1 at the t0 minute timepoint


title: SMY2187_FAP1_t10

source_name: SMY2187 including FAP1 at the t10 minute timepoint


title: SMY2187_FAP1_t15

source_name: SMY2187 including FAP1 at the t15 minute timepoint


title: SMY2187_FAP1_t5

source_name: SMY2187 including FAP1 at the t5 minute timepoint


title: SMY2187_FAP1_t30

source_name: SMY2187 including FAP1 at the t30 minute timepoint


title: SMY2186_ERT1_t90

source_name: SMY2186 including ERT1 at the t90 minute timepoint


title: SMY2186_ERT1_t5

source_name: SMY2186 including ERT1 at the t5 minute timepoint


title: SMY2186_ERT1_t10

source_name: SMY2186 including ERT1 at the t10 minute timepoint


title: SMY2186_ERT1_t30

source_name: SMY2186 including ERT1 at the t30 minute timepoint


title: SMY2186_ERT1_t0

source_name: SMY2186 including ERT1 at the t0 minute timepoint


title: SMY2186_ERT1_t20

source_name: SMY2186 including ERT1 at the t20 minute timepoint


title: SMY2186_ERT1_t15

source_name: SMY2186 including ERT1 at the t15 minute timepoint


title: SMY2185_CTR9_t90

source_name: SMY2185 including CTR9 at the t90 minute timepoint


title: SMY2185_CTR9_t45

source_name: SMY2185 including CTR9 at the t45 minute timepoint


title: SMY2185_CTR9_t10

source_name: SMY2185 including CTR9 at the t10 minute timepoint


title: SMY2185_CTR9_t20

source_name: SMY2185 including CTR9 at the t20 minute timepoint


title: SMY2186_ERT1_t45

source_name: SMY2186 including ERT1 at the t45 minute timepoint


title: SMY2185_CTR9_t30

source_name: SMY2185 including CTR9 at the t30 minute timepoint


title: SMY2187_FAP1_t20

source_name: SMY2187 including FAP1 at the t20 minute timepoint


title: SMY2185_CTR9_t15

source_name: SMY2185 including CTR9 at the t15 minute timepoint


title: SMY2184_CTI6_t90

source_name: SMY2184 including CTI6 at the t90 minute timepoint


title: SMY2185_CTR9_t5

source_name: SMY2185 including CTR9 at the t5 minute timepoint


title: SMY2184_CTI6_t45

source_name: SMY2184 including CTI6 at the t45 minute timepoint


title: SMY2184_CTI6_t10

source_name: SMY2184 including CTI6 at the t10 minute timepoint


title: SMY2185_CTR9_t0

source_name: SMY2185 including CTR9 at the t0 minute timepoint


title: SMY2184_CTI6_t15

source_name: SMY2184 including CTI6 at the t15 minute timepoint


title: SMY2184_CTI6_t30

source_name: SMY2184 including CTI6 at the t30 minute timepoint


title: SMY2183_CRF1_t30

source_name: SMY2183 including CRF1 at the t30 minute timepoint


title: SMY2183_CRF1_t20

source_name: SMY2183 including CRF1 at the t20 minute timepoint


title: SMY2183_CRF1_t90

source_name: SMY2183 including CRF1 at the t90 minute timepoint


title: SMY2183_CRF1_t45

source_name: SMY2183 including CRF1 at the t45 minute timepoint


title: SMY2184_CTI6_t0

source_name: SMY2184 including CTI6 at the t0 minute timepoint


title: SMY2183_CRF1_t15

source_name: SMY2183 including CRF1 at the t15 minute timepoint


title: SMY2183_CRF1_t10

source_name: SMY2183 including CRF1 at the t10 minute timepoint


title: SMY2183_CRF1_t5

source_name: SMY2183 including CRF1 at the t5 minute timepoint


title: SMY2183_CRF1_t0

source_name: SMY2183 including CRF1 at the t0 minute timepoint


title: SMY2182_CAM1_t15

source_name: SMY2182 including CAM1 at the t15 minute timepoint


title: SMY2182_CAM1_t20

source_name: SMY2182 including CAM1 at the t20 minute timepoint


title: SMY2182_CAM1_t30

source_name: SMY2182 including CAM1 at the t30 minute timepoint


title: SMY2182_CAM1_t10

source_name: SMY2182 including CAM1 at the t10 minute timepoint


title: SMY2182_CAM1_t5

source_name: SMY2182 including CAM1 at the t5 minute timepoint


title: SMY2182_CAM1_t0

source_name: SMY2182 including CAM1 at the t0 minute timepoint


title: SMY2181_BMH1_t30

source_name: SMY2181 including BMH1 at the t30 minute timepoint


title: SMY2181_BMH1_t45

source_name: SMY2181 including BMH1 at the t45 minute timepoint


title: SMY2182_CAM1_t90

source_name: SMY2182 including CAM1 at the t90 minute timepoint


title: SMY2181_BMH1_t90

source_name: SMY2181 including BMH1 at the t90 minute timepoint


title: SMY2182_CAM1_t45

source_name: SMY2182 including CAM1 at the t45 minute timepoint


title: SMY2181_BMH1_t10

source_name: SMY2181 including BMH1 at the t10 minute timepoint


title: SMY2181_BMH1_t20

source_name: SMY2181 including BMH1 at the t20 minute timepoint


title: SMY2181_BMH1_t15

source_name: SMY2181 including BMH1 at the t15 minute timepoint


title: SMY2181_BMH1_t5

source_name: SMY2181 including BMH1 at the t5 minute timepoint


title: SMY2180_BDF2_t45

source_name: SMY2180 including BDF2 at the t45 minute timepoint


title: SMY2180_BDF2_t90

source_name: SMY2180 including BDF2 at the t90 minute timepoint


title: SMY2180_BDF2_t5

source_name: SMY2180 including BDF2 at the t5 minute timepoint


title: SMY2180_BDF2_t20

source_name: SMY2180 including BDF2 at the t20 minute timepoint


title: SMY2180_BDF2_t15

source_name: SMY2180 including BDF2 at the t15 minute timepoint


title: SMY2180_BDF2_t10

source_name: SMY2180 including BDF2 at the t10 minute timepoint


title: SMY2179_ARO80_t90

source_name: SMY2179 including ARO80 at the t90 minute timepoint


title: SMY2179_ARO80_t5

source_name: SMY2179 including ARO80 at the t5 minute timepoint


title: SMY2179_ARO80_t30

source_name: SMY2179 including ARO80 at the t30 minute timepoint


title: SMY2179_ARO80_t20

source_name: SMY2179 including ARO80 at the t20 minute timepoint


title: SMY2181_BMH1_t0

source_name: SMY2181 including BMH1 at the t0 minute timepoint


title: SMY2179_ARO80_t45

source_name: SMY2179 including ARO80 at the t45 minute timepoint


title: SMY2180_BDF2_t30

source_name: SMY2180 including BDF2 at the t30 minute timepoint


title: SMY2179_ARO80_t15

source_name: SMY2179 including ARO80 at the t15 minute timepoint


title: SMY2184_CTI6_t20

source_name: SMY2184 including CTI6 at the t20 minute timepoint


title: SMY2174_PHD1_t45

source_name: SMY2174 including PHD1 at the t45 minute timepoint


title: SMY2174_PHD1_t20

source_name: SMY2174 including PHD1 at the t20 minute timepoint


title: SMY2174_PHD1_t90

source_name: SMY2174 including PHD1 at the t90 minute timepoint


title: SMY2174_PHD1_t0

source_name: SMY2174 including PHD1 at the t0 minute timepoint


title: SMY2174_PHD1_t15

source_name: SMY2174 including PHD1 at the t15 minute timepoint


title: SMY2174_PHD1_t30

source_name: SMY2174 including PHD1 at the t30 minute timepoint


title: SMY2166_SRB2_t45

source_name: SMY2166 including SRB2 at the t45 minute timepoint


title: SMY2166_SRB2_t20

source_name: SMY2166 including SRB2 at the t20 minute timepoint


title: SMY2166_SRB2_t30

source_name: SMY2166 including SRB2 at the t30 minute timepoint


title: SMY2179_ARO80_t0

source_name: SMY2179 including ARO80 at the t0 minute timepoint


title: SMY2179_ARO80_t10

source_name: SMY2179 including ARO80 at the t10 minute timepoint


title: SMY2174_PHD1_t10

source_name: SMY2174 including PHD1 at the t10 minute timepoint


title: SMY2166_SRB2_t15

source_name: SMY2166 including SRB2 at the t15 minute timepoint


title: SMY2180_BDF2_t0

source_name: SMY2180 including BDF2 at the t0 minute timepoint


title: SMY2166_SRB2_t5

source_name: SMY2166 including SRB2 at the t5 minute timepoint


title: SMY2166_SRB2_t0

source_name: SMY2166 including SRB2 at the t0 minute timepoint


title: SMY2164_RTG2_t90

source_name: SMY2164 including RTG2 at the t90 minute timepoint


title: SMY2174_PHD1_t5

source_name: SMY2174 including PHD1 at the t5 minute timepoint


title: SMY2166_SRB2_t10

source_name: SMY2166 including SRB2 at the t10 minute timepoint


title: SMY2164_RTG2_t45

source_name: SMY2164 including RTG2 at the t45 minute timepoint


title: SMY2164_RTG2_t15

source_name: SMY2164 including RTG2 at the t15 minute timepoint


title: SMY2164_RTG2_t5

source_name: SMY2164 including RTG2 at the t5 minute timepoint


title: SMY2164_RTG2_t20

source_name: SMY2164 including RTG2 at the t20 minute timepoint


title: SMY2166_SRB2_t90

source_name: SMY2166 including SRB2 at the t90 minute timepoint


title: SMY2164_RTG2_t10

source_name: SMY2164 including RTG2 at the t10 minute timepoint


title: SMY2164_RTG2_t30

source_name: SMY2164 including RTG2 at the t30 minute timepoint


title: SMY2164_RTG2_t0

source_name: SMY2164 including RTG2 at the t0 minute timepoint


title: SMY2178_ADA2_t20

source_name: SMY2178 including ADA2 at the t20 minute timepoint


title: SMY2178_ADA2_t100

source_name: SMY2178 including ADA2 at the t100 minute timepoint


title: SMY2178_ADA2_t15

source_name: SMY2178 including ADA2 at the t15 minute timepoint


title: SMY2178_ADA2_t10

source_name: SMY2178 including ADA2 at the t10 minute timepoint


title: SMY2178_ADA2_t5

source_name: SMY2178 including ADA2 at the t5 minute timepoint


title: SMY2176_SGF29_t45

source_name: SMY2176 including SGF29 at the t45 minute timepoint


title: SMY2176_SGF29_t100

source_name: SMY2176 including SGF29 at the t100 minute timepoint


title: SMY2178_ADA2_t45

source_name: SMY2178 including ADA2 at the t45 minute timepoint


title: SMY2177_SPT4_t20

source_name: SMY2177 including SPT4 at the t20 minute timepoint


title: SMY2177_SPT4_t45

source_name: SMY2177 including SPT4 at the t45 minute timepoint


title: SMY2177_SPT4_t0

source_name: SMY2177 including SPT4 at the t0 minute timepoint


title: SMY2178_ADA2_t30

source_name: SMY2178 including ADA2 at the t30 minute timepoint


title: SMY2177_SPT4_t10

source_name: SMY2177 including SPT4 at the t10 minute timepoint


title: SMY2177_SPT4_t30

source_name: SMY2177 including SPT4 at the t30 minute timepoint


title: SMY2177_SPT4_t90

source_name: SMY2177 including SPT4 at the t90 minute timepoint


title: SMY2177_SPT4_t5

source_name: SMY2177 including SPT4 at the t5 minute timepoint


title: SMY2177_SPT4_t15

source_name: SMY2177 including SPT4 at the t15 minute timepoint


title: SMY2178_ADA2_t0

source_name: SMY2178 including ADA2 at the t0 minute timepoint


title: SMY2176_SGF29_t15

source_name: SMY2176 including SGF29 at the t15 minute timepoint


title: SMY2176_SGF29_t30

source_name: SMY2176 including SGF29 at the t30 minute timepoint


title: SMY2176_SGF29_t20

source_name: SMY2176 including SGF29 at the t20 minute timepoint


title: SMY2176_SGF29_t0

source_name: SMY2176 including SGF29 at the t0 minute timepoint


title: SMY2175_RTF1_t30

source_name: SMY2175 including RTF1 at the t30 minute timepoint


title: SMY2175_RTF1_t45

source_name: SMY2175 including RTF1 at the t45 minute timepoint


title: SMY2175_RTF1_t90

source_name: SMY2175 including RTF1 at the t90 minute timepoint


title: SMY2176_SGF29_t5

source_name: SMY2176 including SGF29 at the t5 minute timepoint


title: SMY2176_SGF29_t10

source_name: SMY2176 including SGF29 at the t10 minute timepoint


title: SMY2175_RTF1_t0

source_name: SMY2175 including RTF1 at the t0 minute timepoint


title: SMY2171_GCR2_t90

source_name: SMY2171 including GCR2 at the t90 minute timepoint


title: SMY2171_GCR2_t45

source_name: SMY2171 including GCR2 at the t45 minute timepoint


title: SMY2175_RTF1_t20

source_name: SMY2175 including RTF1 at the t20 minute timepoint


title: SMY2175_RTF1_t15

source_name: SMY2175 including RTF1 at the t15 minute timepoint


title: SMY2171_GCR2_t30

source_name: SMY2171 including GCR2 at the t30 minute timepoint


title: SMY2171_GCR2_t20

source_name: SMY2171 including GCR2 at the t20 minute timepoint


title: SMY2171_GCR2_t10

source_name: SMY2171 including GCR2 at the t10 minute timepoint


title: SMY2171_GCR2_t15

source_name: SMY2171 including GCR2 at the t15 minute timepoint


title: SMY2184_CTI6_t5

source_name: SMY2184 including CTI6 at the t5 minute timepoint


title: SMY2175_RTF1_t5

source_name: SMY2175 including RTF1 at the t5 minute timepoint


title: SMY2169_CUP9_t30

source_name: SMY2169 including CUP9 at the t30 minute timepoint


title: SMY2169_CUP9_t5

source_name: SMY2169 including CUP9 at the t5 minute timepoint


title: SMY2169_CUP9_t10

source_name: SMY2169 including CUP9 at the t10 minute timepoint


title: SMY2171_GCR2_t0

source_name: SMY2171 including GCR2 at the t0 minute timepoint


title: SMY2175_RTF1_t10

source_name: SMY2175 including RTF1 at the t10 minute timepoint


title: SMY2169_CUP9_t15

source_name: SMY2169 including CUP9 at the t15 minute timepoint


title: SMY2169_CUP9_t0

source_name: SMY2169 including CUP9 at the t0 minute timepoint


title: SMY2168_SUT2_t90

source_name: SMY2168 including SUT2 at the t90 minute timepoint


title: SMY2169_CUP9_t45

source_name: SMY2169 including CUP9 at the t45 minute timepoint


title: SMY2168_SUT2_t15

source_name: SMY2168 including SUT2 at the t15 minute timepoint


title: SMY2169_CUP9_t90

source_name: SMY2169 including CUP9 at the t90 minute timepoint


title: SMY2168_SUT2_t10

source_name: SMY2168 including SUT2 at the t10 minute timepoint


title: SMY2169_CUP9_t20

source_name: SMY2169 including CUP9 at the t20 minute timepoint


title: SMY2168_SUT2_t45

source_name: SMY2168 including SUT2 at the t45 minute timepoint


title: SMY2167_SUT1_t90

source_name: SMY2167 including SUT1 at the t90 minute timepoint


title: SMY2168_SUT2_t20

source_name: SMY2168 including SUT2 at the t20 minute timepoint


title: SMY2171_GCR2_t5

source_name: SMY2171 including GCR2 at the t5 minute timepoint


title: SMY2168_SUT2_t0

source_name: SMY2168 including SUT2 at the t0 minute timepoint


title: SMY2167_SUT1_t20

source_name: SMY2167 including SUT1 at the t20 minute timepoint


title: SMY2167_SUT1_t15

source_name: SMY2167 including SUT1 at the t15 minute timepoint


title: SMY2168_SUT2_t5

source_name: SMY2168 including SUT2 at the t5 minute timepoint


title: SMY2168_SUT2_t30

source_name: SMY2168 including SUT2 at the t30 minute timepoint


title: SMY2167_SUT1_t10

source_name: SMY2167 including SUT1 at the t10 minute timepoint


title: SMY2165_SIN4_t90

source_name: SMY2165 including SIN4 at the t90 minute timepoint


title: SMY2167_SUT1_t45

source_name: SMY2167 including SUT1 at the t45 minute timepoint


title: SMY2167_SUT1_t0

source_name: SMY2167 including SUT1 at the t0 minute timepoint


title: SMY2167_SUT1_t30

source_name: SMY2167 including SUT1 at the t30 minute timepoint


title: SMY2163_RPD3_t20

source_name: SMY2163 including RPD3 at the t20 minute timepoint


title: SMY2163_RPD3_t45

source_name: SMY2163 including RPD3 at the t45 minute timepoint


title: SMY2163_RPD3_t90

source_name: SMY2163 including RPD3 at the t90 minute timepoint


title: SMY2165_SIN4_t10

source_name: SMY2165 including SIN4 at the t10 minute timepoint


title: SMY2167_SUT1_t5

source_name: SMY2167 including SUT1 at the t5 minute timepoint


title: SMY2165_SIN4_t15

source_name: SMY2165 including SIN4 at the t15 minute timepoint


title: SMY2188_FHL1_t90

source_name: SMY2188 including FHL1 at the t90 minute timepoint


title: SMY2165_SIN4_t20

source_name: SMY2165 including SIN4 at the t20 minute timepoint


title: SMY2163_RPD3_t30

source_name: SMY2163 including RPD3 at the t30 minute timepoint


title: SMY2188_FHL1_t0

source_name: SMY2188 including FHL1 at the t0 minute timepoint


title: SMY2188_FHL1_t30

source_name: SMY2188 including FHL1 at the t30 minute timepoint


title: SMY2188_FHL1_t45

source_name: SMY2188 including FHL1 at the t45 minute timepoint


title: SMY2165_SIN4_t0

source_name: SMY2165 including SIN4 at the t0 minute timepoint


title: SMY2165_SIN4_t30

source_name: SMY2165 including SIN4 at the t30 minute timepoint


title: SMY2188_FHL1_t15

source_name: SMY2188 including FHL1 at the t15 minute timepoint


title: SMY2188_FHL1_t20

source_name: SMY2188 including FHL1 at the t20 minute timepoint


title: SMY2188_FHL1_t10

source_name: SMY2188 including FHL1 at the t10 minute timepoint


title: SMY2165_SIN4_t5

source_name: SMY2165 including SIN4 at the t5 minute timepoint


title: SMY2165_SIN4_t45

source_name: SMY2165 including SIN4 at the t45 minute timepoint


title: SMY2163_RPD3_t15

source_name: SMY2163 including RPD3 at the t15 minute timepoint


title: SMY2163_RPD3_t10

source_name: SMY2163 including RPD3 at the t10 minute timepoint


title: SMY2188_FHL1_t5

source_name: SMY2188 including FHL1 at the t5 minute timepoint


title: SMY2163_RPD3_t0

source_name: SMY2163 including RPD3 at the t0 minute timepoint


title: SMY2163_RPD3_t5

source_name: SMY2163 including RPD3 at the t5 minute timepoint


title: SMY2162_ROX3_t90

source_name: SMY2162 including ROX3 at the t90 minute timepoint


title: SMY2162_ROX3_t30

source_name: SMY2162 including ROX3 at the t30 minute timepoint


title: SMY2162_ROX3_t20

source_name: SMY2162 including ROX3 at the t20 minute timepoint


title: SMY2162_ROX3_t15

source_name: SMY2162 including ROX3 at the t15 minute timepoint


title: SMY2162_ROX3_t10

source_name: SMY2162 including ROX3 at the t10 minute timepoint


title: SMY2162_ROX3_t45

source_name: SMY2162 including ROX3 at the t45 minute timepoint


title: SMY2162_ROX3_t5

source_name: SMY2162 including ROX3 at the t5 minute timepoint


title: SMY2161_OAF3_t20

source_name: SMY2161 including OAF3 at the t20 minute timepoint


title: SMY2162_ROX3_t0

source_name: SMY2162 including ROX3 at the t0 minute timepoint


title: SMY2161_OAF3_t90

source_name: SMY2161 including OAF3 at the t90 minute timepoint


title: SMY2161_OAF3_t30

source_name: SMY2161 including OAF3 at the t30 minute timepoint


title: SMY2159_MED2_t30

source_name: SMY2159 including MED2 at the t30 minute timepoint


title: SMY2161_OAF3_t45

source_name: SMY2161 including OAF3 at the t45 minute timepoint


title: SMY2159_MED2_t20

source_name: SMY2159 including MED2 at the t20 minute timepoint


title: SMY2161_OAF3_t5

source_name: SMY2161 including OAF3 at the t5 minute timepoint


title: SMY2159_MED2_t5

source_name: SMY2159 including MED2 at the t5 minute timepoint


title: SMY2161_OAF3_t0

source_name: SMY2161 including OAF3 at the t0 minute timepoint


title: SMY2159_MED2_t10

source_name: SMY2159 including MED2 at the t10 minute timepoint


title: SMY2157_LOT6_t45

source_name: SMY2157 including LOT6 at the t45 minute timepoint


title: SMY2159_MED2_t90

source_name: SMY2159 including MED2 at the t90 minute timepoint


title: SMY2157_LOT6_t10

source_name: SMY2157 including LOT6 at the t10 minute timepoint


title: SMY2159_MED2_t15

source_name: SMY2159 including MED2 at the t15 minute timepoint


title: SMY2157_LOT6_t20

source_name: SMY2157 including LOT6 at the t20 minute timepoint


title: SMY2157_LOT6_t30

source_name: SMY2157 including LOT6 at the t30 minute timepoint


title: SMY2157_LOT6_t5

source_name: SMY2157 including LOT6 at the t5 minute timepoint


title: SMY2159_MED2_t0

source_name: SMY2159 including MED2 at the t0 minute timepoint


title: SMY2157_LOT6_t15

source_name: SMY2157 including LOT6 at the t15 minute timepoint


title: SMY2157_LOT6_t0

source_name: SMY2157 including LOT6 at the t0 minute timepoint


title: SMY2161_OAF3_t15

source_name: SMY2161 including OAF3 at the t15 minute timepoint


title: SMY2155_HMRA1_t90

source_name: SMY2155 including HMRA1 at the t90 minute timepoint


title: SMY2155_HMRA1_t45

source_name: SMY2155 including HMRA1 at the t45 minute timepoint


title: SMY2161_OAF3_t10

source_name: SMY2161 including OAF3 at the t10 minute timepoint


title: SMY2155_HMRA1_t30

source_name: SMY2155 including HMRA1 at the t30 minute timepoint


title: SMY2155_HMRA1_t15

source_name: SMY2155 including HMRA1 at the t15 minute timepoint


title: SMY2155_HMRA1_t10

source_name: SMY2155 including HMRA1 at the t10 minute timepoint


title: SMY2155_HMRA1_t20

source_name: SMY2155 including HMRA1 at the t20 minute timepoint


title: SMY2155_HMRA1_t0

source_name: SMY2155 including HMRA1 at the t0 minute timepoint


title: SMY2147_GAL11_t15

source_name: SMY2147 including GAL11 at the t15 minute timepoint


title: SMY2147_GAL11_t45

source_name: SMY2147 including GAL11 at the t45 minute timepoint


title: SMY2147_GAL11_t10

source_name: SMY2147 including GAL11 at the t10 minute timepoint


title: SMY2147_GAL11_t20

source_name: SMY2147 including GAL11 at the t20 minute timepoint


title: SMY2124_TEA1_t90

source_name: SMY2124 including TEA1 at the t90 minute timepoint


title: SMY2147_GAL11_t0

source_name: SMY2147 including GAL11 at the t0 minute timepoint


title: SMY2147_GAL11_t5

source_name: SMY2147 including GAL11 at the t5 minute timepoint


title: SMY2154_HIR2_t15

source_name: SMY2154 including HIR2 at the t15 minute timepoint


title: SMY2154_HIR2_t0

source_name: SMY2154 including HIR2 at the t0 minute timepoint


title: SMY2147_GAL11_t30

source_name: SMY2147 including GAL11 at the t30 minute timepoint


title: SMY2154_HIR2_t5

source_name: SMY2154 including HIR2 at the t5 minute timepoint


title: SMY2154_HIR2_t10

source_name: SMY2154 including HIR2 at the t10 minute timepoint


title: SMY2124_TEA1_t20

source_name: SMY2124 including TEA1 at the t20 minute timepoint


title: SMY2154_HIR2_t90

source_name: SMY2154 including HIR2 at the t90 minute timepoint


title: SMY2124_TEA1_t45

source_name: SMY2124 including TEA1 at the t45 minute timepoint


title: SMY2154_HIR2_t45

source_name: SMY2154 including HIR2 at the t45 minute timepoint


title: SMY2154_HIR2_t30

source_name: SMY2154 including HIR2 at the t30 minute timepoint


title: SMY2154_HIR2_t20

source_name: SMY2154 including HIR2 at the t20 minute timepoint


title: SMY2124_TEA1_t30

source_name: SMY2124 including TEA1 at the t30 minute timepoint


title: SMY2124_TEA1_t15

source_name: SMY2124 including TEA1 at the t15 minute timepoint


title: SMY2124_TEA1_t10

source_name: SMY2124 including TEA1 at the t10 minute timepoint


title: SMY2155_HMRA1_t5

source_name: SMY2155 including HMRA1 at the t5 minute timepoint


title: SMY2147_GAL11_t90

source_name: SMY2147 including GAL11 at the t90 minute timepoint


title: SMY2124_TEA1_t5

source_name: SMY2124 including TEA1 at the t5 minute timepoint


title: SMY2124_TEA1_t0

source_name: SMY2124 including TEA1 at the t0 minute timepoint


title: SMY2207_RDS2_t90

source_name: SMY2207 including RDS2 at the t90 minute timepoint


title: SMY2207_RDS2_t45

source_name: SMY2207 including RDS2 at the t45 minute timepoint


title: SMY2207_RDS2_t15

source_name: SMY2207 including RDS2 at the t15 minute timepoint


title: SMY2207_RDS2_t10

source_name: SMY2207 including RDS2 at the t10 minute timepoint


title: SMY2207_RDS2_t20

source_name: SMY2207 including RDS2 at the t20 minute timepoint


title: SMY2207_RDS2_t0

source_name: SMY2207 including RDS2 at the t0 minute timepoint


title: SMY2170_DIG1_t90

source_name: SMY2170 including DIG1 at the t90 minute timepoint


title: SMY2207_RDS2_t5

source_name: SMY2207 including RDS2 at the t5 minute timepoint


title: SMY2170_DIG1_t10

source_name: SMY2170 including DIG1 at the t10 minute timepoint


title: SMY2170_DIG1_t5

source_name: SMY2170 including DIG1 at the t5 minute timepoint


title: SMY2160_MGA1_t15

source_name: SMY2160 including MGA1 at the t15 minute timepoint


title: SMY2170_DIG1_t0

source_name: SMY2170 including DIG1 at the t0 minute timepoint


title: SMY2159_MED2_t45

source_name: SMY2159 including MED2 at the t45 minute timepoint


title: SMY2170_DIG1_t15

source_name: SMY2170 including DIG1 at the t15 minute timepoint


title: SMY2160_MGA1_t30

source_name: SMY2160 including MGA1 at the t30 minute timepoint


title: SMY2160_MGA1_t45

source_name: SMY2160 including MGA1 at the t45 minute timepoint


title: SMY2160_MGA1_t20

source_name: SMY2160 including MGA1 at the t20 minute timepoint


title: SMY2170_DIG1_t20

source_name: SMY2170 including DIG1 at the t20 minute timepoint


title: SMY2160_MGA1_t0

source_name: SMY2160 including MGA1 at the t0 minute timepoint


title: SMY2160_MGA1_t5

source_name: SMY2160 including MGA1 at the t5 minute timepoint


title: SMY2153_HAA1_t45

source_name: SMY2153 including HAA1 at the t45 minute timepoint


title: SMY2207_RDS2_t30

source_name: SMY2207 including RDS2 at the t30 minute timepoint


title: SMY2160_MGA1_t90

source_name: SMY2160 including MGA1 at the t90 minute timepoint


title: SMY2153_HAA1_t30

source_name: SMY2153 including HAA1 at the t30 minute timepoint


title: SMY2153_HAA1_t90

source_name: SMY2153 including HAA1 at the t90 minute timepoint


title: SMY2170_DIG1_t30

source_name: SMY2170 including DIG1 at the t30 minute timepoint


title: SMY2153_HAA1_t15

source_name: SMY2153 including HAA1 at the t15 minute timepoint


title: SMY2157_LOT6_t90

source_name: SMY2157 including LOT6 at the t90 minute timepoint


title: SMY2160_MGA1_t10

source_name: SMY2160 including MGA1 at the t10 minute timepoint


title: SMY2150_GAT4_t90

source_name: SMY2150 including GAT4 at the t90 minute timepoint


title: SMY2150_GAT4_t20

source_name: SMY2150 including GAT4 at the t20 minute timepoint


title: SMY2150_GAT4_t45

source_name: SMY2150 including GAT4 at the t45 minute timepoint


title: SMY2150_GAT4_t30

source_name: SMY2150 including GAT4 at the t30 minute timepoint


title: SMY2153_HAA1_t5

source_name: SMY2153 including HAA1 at the t5 minute timepoint


title: SMY2150_GAT4_t10

source_name: SMY2150 including GAT4 at the t10 minute timepoint


title: SMY2150_GAT4_t15

source_name: SMY2150 including GAT4 at the t15 minute timepoint


title: SMY2149_GAT3_t90

source_name: SMY2149 including GAT3 at the t90 minute timepoint


title: SMY2149_GAT3_t20

source_name: SMY2149 including GAT3 at the t20 minute timepoint


title: SMY2149_GAT3_t45

source_name: SMY2149 including GAT3 at the t45 minute timepoint


title: SMY2150_GAT4_t5

source_name: SMY2150 including GAT4 at the t5 minute timepoint


title: SMY2150_GAT4_t0

source_name: SMY2150 including GAT4 at the t0 minute timepoint


title: SMY2148_GAL80_t45

source_name: SMY2148 including GAL80 at the t45 minute timepoint


title: SMY2149_GAT3_t30

source_name: SMY2149 including GAT3 at the t30 minute timepoint


title: SMY2149_GAT3_t15

source_name: SMY2149 including GAT3 at the t15 minute timepoint


title: SMY2148_GAL80_t90

source_name: SMY2148 including GAL80 at the t90 minute timepoint


title: SMY2149_GAT3_t0

source_name: SMY2149 including GAT3 at the t0 minute timepoint


title: SMY2148_GAL80_t0

source_name: SMY2148 including GAL80 at the t0 minute timepoint


title: SMY2149_GAT3_t10

source_name: SMY2149 including GAT3 at the t10 minute timepoint


title: SMY2148_GAL80_t5

source_name: SMY2148 including GAL80 at the t5 minute timepoint


title: SMY2148_GAL80_t10

source_name: SMY2148 including GAL80 at the t10 minute timepoint


title: SMY2148_GAL80_t15

source_name: SMY2148 including GAL80 at the t15 minute timepoint


title: SMY2153_HAA1_t10

source_name: SMY2153 including HAA1 at the t10 minute timepoint


title: SMY2146_DST1_t90

source_name: SMY2146 including DST1 at the t90 minute timepoint


title: SMY2149_GAT3_t5

source_name: SMY2149 including GAT3 at the t5 minute timepoint


title: SMY2148_GAL80_t30

source_name: SMY2148 including GAL80 at the t30 minute timepoint


title: SMY2148_GAL80_t20

source_name: SMY2148 including GAL80 at the t20 minute timepoint


title: SMY2146_DST1_t45

source_name: SMY2146 including DST1 at the t45 minute timepoint


title: SMY2153_HAA1_t0

source_name: SMY2153 including HAA1 at the t0 minute timepoint


title: SMY2146_DST1_t30

source_name: SMY2146 including DST1 at the t30 minute timepoint


title: SMY2146_DST1_t20

source_name: SMY2146 including DST1 at the t20 minute timepoint


title: SMY2146_DST1_t5

source_name: SMY2146 including DST1 at the t5 minute timepoint


title: SMY2146_DST1_t10

source_name: SMY2146 including DST1 at the t10 minute timepoint


title: SMY2145_DIG2_t45

source_name: SMY2145 including DIG2 at the t45 minute timepoint


title: SMY2146_DST1_t0

source_name: SMY2146 including DST1 at the t0 minute timepoint


title: SMY2145_DIG2_t10

source_name: SMY2145 including DIG2 at the t10 minute timepoint


title: SMY2143_CSE2_t0

source_name: SMY2143 including CSE2 at the t0 minute timepoint


title: SMY2145_DIG2_t30

source_name: SMY2145 including DIG2 at the t30 minute timepoint


title: SMY2145_DIG2_t20

source_name: SMY2145 including DIG2 at the t20 minute timepoint


title: SMY2143_CSE2_t5

source_name: SMY2143 including CSE2 at the t5 minute timepoint


title: SMY2143_CSE2_t10

source_name: SMY2143 including CSE2 at the t10 minute timepoint


title: SMY2145_DIG2_t5

source_name: SMY2145 including DIG2 at the t5 minute timepoint


title: SMY2143_CSE2_t30

source_name: SMY2143 including CSE2 at the t30 minute timepoint


title: SMY2143_CSE2_t15

source_name: SMY2143 including CSE2 at the t15 minute timepoint


title: SMY2141_CDC73_t90

source_name: SMY2141 including CDC73 at the t90 minute timepoint


title: SMY2143_CSE2_t20

source_name: SMY2143 including CSE2 at the t20 minute timepoint


title: SMY2145_DIG2_t90

source_name: SMY2145 including DIG2 at the t90 minute timepoint


title: SMY2145_DIG2_t0

source_name: SMY2145 including DIG2 at the t0 minute timepoint


title: SMY2141_CDC73_t20

source_name: SMY2141 including CDC73 at the t20 minute timepoint


title: SMY2141_CDC73_t15

source_name: SMY2141 including CDC73 at the t15 minute timepoint


title: SMY2141_CDC73_t10

source_name: SMY2141 including CDC73 at the t10 minute timepoint


title: SMY2143_CSE2_t90

source_name: SMY2143 including CSE2 at the t90 minute timepoint


title: SMY2143_CSE2_t45

source_name: SMY2143 including CSE2 at the t45 minute timepoint


title: SMY2101_PPR1_t90

source_name: SMY2101 including PPR1 at the t90 minute timepoint


title: SMY2141_CDC73_t0

source_name: SMY2141 including CDC73 at the t0 minute timepoint


title: SMY2141_CDC73_t5

source_name: SMY2141 including CDC73 at the t5 minute timepoint


title: SMY2141_CDC73_t30

source_name: SMY2141 including CDC73 at the t30 minute timepoint


title: SMY2141_CDC73_t45

source_name: SMY2141 including CDC73 at the t45 minute timepoint


title: SMY2145_DIG2_t15

source_name: SMY2145 including DIG2 at the t15 minute timepoint


title: SMY2101_PPR1_t30

source_name: SMY2101 including PPR1 at the t30 minute timepoint


title: SMY2101_PPR1_t20

source_name: SMY2101 including PPR1 at the t20 minute timepoint


title: SMY2101_PPR1_t45

source_name: SMY2101 including PPR1 at the t45 minute timepoint


title: SMY2101_PPR1_t15

source_name: SMY2101 including PPR1 at the t15 minute timepoint


title: SMY2101_PPR1_t10

source_name: SMY2101 including PPR1 at the t10 minute timepoint


title: SMY2156_HOT1_t45

source_name: SMY2156 including HOT1 at the t45 minute timepoint


title: SMY2156_HOT1_t30

source_name: SMY2156 including HOT1 at the t30 minute timepoint


title: SMY2170_DIG1_t45

source_name: SMY2170 including DIG1 at the t45 minute timepoint


title: SMY2156_HOT1_t90

source_name: SMY2156 including HOT1 at the t90 minute timepoint


title: SMY2132_YRR1_t90

source_name: SMY2132 including YRR1 at the t90 minute timepoint


title: SMY2156_HOT1_t0

source_name: SMY2156 including HOT1 at the t0 minute timepoint


title: SMY2156_HOT1_t15

source_name: SMY2156 including HOT1 at the t15 minute timepoint


title: SMY2156_HOT1_t20

source_name: SMY2156 including HOT1 at the t20 minute timepoint


title: SMY2156_HOT1_t5

source_name: SMY2156 including HOT1 at the t5 minute timepoint


title: SMY2132_YRR1_t30

source_name: SMY2132 including YRR1 at the t30 minute timepoint


title: SMY2132_YRR1_t0

source_name: SMY2132 including YRR1 at the t0 minute timepoint


title: SMY2101_PPR1_t0

source_name: SMY2101 including PPR1 at the t0 minute timepoint


title: SMY2101_PPR1_t5

source_name: SMY2101 including PPR1 at the t5 minute timepoint


title: SMY2132_YRR1_t5

source_name: SMY2132 including YRR1 at the t5 minute timepoint


title: SMY2127_UME6_t20

source_name: SMY2127 including UME6 at the t20 minute timepoint


title: SMY2127_UME6_t30

source_name: SMY2127 including UME6 at the t30 minute timepoint


title: SMY2127_UME6_t45

source_name: SMY2127 including UME6 at the t45 minute timepoint


title: SMY2132_YRR1_t15

source_name: SMY2132 including YRR1 at the t15 minute timepoint


title: SMY2125_TEC1_t90

source_name: SMY2125 including TEC1 at the t90 minute timepoint


title: SMY2132_YRR1_t10

source_name: SMY2132 including YRR1 at the t10 minute timepoint


title: SMY2127_UME6_t5

source_name: SMY2127 including UME6 at the t5 minute timepoint


title: SMY2127_UME6_t10

source_name: SMY2127 including UME6 at the t10 minute timepoint


title: SMY2127_UME6_t0

source_name: SMY2127 including UME6 at the t0 minute timepoint


title: SMY2156_HOT1_t10

source_name: SMY2156 including HOT1 at the t10 minute timepoint


title: SMY2125_TEC1_t45

source_name: SMY2125 including TEC1 at the t45 minute timepoint


title: SMY2132_YRR1_t45

source_name: SMY2132 including YRR1 at the t45 minute timepoint


title: SMY2132_YRR1_t20

source_name: SMY2132 including YRR1 at the t20 minute timepoint


title: SMY2125_TEC1_t30

source_name: SMY2125 including TEC1 at the t30 minute timepoint


title: SMY2125_TEC1_t20

source_name: SMY2125 including TEC1 at the t20 minute timepoint


title: SMY2125_TEC1_t15

source_name: SMY2125 including TEC1 at the t15 minute timepoint


title: SMY2125_TEC1_t5

source_name: SMY2125 including TEC1 at the t5 minute timepoint


title: SMY2125_TEC1_t0

source_name: SMY2125 including TEC1 at the t0 minute timepoint


title: SMY2123_SWI5_t90

source_name: SMY2123 including SWI5 at the t90 minute timepoint


title: SMY2123_SWI5_t15

source_name: SMY2123 including SWI5 at the t15 minute timepoint


title: SMY2123_SWI5_t45

source_name: SMY2123 including SWI5 at the t45 minute timepoint


title: SMY2123_SWI5_t20

source_name: SMY2123 including SWI5 at the t20 minute timepoint


title: SMY2123_SWI5_t30

source_name: SMY2123 including SWI5 at the t30 minute timepoint


title: SMY2123_SWI5_t10

source_name: SMY2123 including SWI5 at the t10 minute timepoint


title: SMY2123_SWI5_t5

source_name: SMY2123 including SWI5 at the t5 minute timepoint


title: SMY2116_SMP1_t20

source_name: SMY2116 including SMP1 at the t20 minute timepoint


title: SMY2116_SMP1_t30

source_name: SMY2116 including SMP1 at the t30 minute timepoint


title: SMY2116_SMP1_t90

source_name: SMY2116 including SMP1 at the t90 minute timepoint


title: SMY2116_SMP1_t15

source_name: SMY2116 including SMP1 at the t15 minute timepoint


title: SMY2123_SWI5_t0

source_name: SMY2123 including SWI5 at the t0 minute timepoint


title: SMY2116_SMP1_t10

source_name: SMY2116 including SMP1 at the t10 minute timepoint


title: SMY2115_SKO1_t45

source_name: SMY2115 including SKO1 at the t45 minute timepoint


title: SMY2125_TEC1_t10

source_name: SMY2125 including TEC1 at the t10 minute timepoint


title: SMY2116_SMP1_t5

source_name: SMY2116 including SMP1 at the t5 minute timepoint


title: SMY2116_SMP1_t0

source_name: SMY2116 including SMP1 at the t0 minute timepoint


title: SMY2115_SKO1_t90

source_name: SMY2115 including SKO1 at the t90 minute timepoint


title: SMY2115_SKO1_t30

source_name: SMY2115 including SKO1 at the t30 minute timepoint


title: SMY2115_SKO1_t10

source_name: SMY2115 including SKO1 at the t10 minute timepoint


title: SMY2112_SFL1_t90

source_name: SMY2112 including SFL1 at the t90 minute timepoint


title: SMY2116_SMP1_t45

source_name: SMY2116 including SMP1 at the t45 minute timepoint


title: SMY2112_SFL1_t45

source_name: SMY2112 including SFL1 at the t45 minute timepoint


title: SMY2112_SFL1_t30

source_name: SMY2112 including SFL1 at the t30 minute timepoint


title: SMY2115_SKO1_t15

source_name: SMY2115 including SKO1 at the t15 minute timepoint


title: SMY2115_SKO1_t20

source_name: SMY2115 including SKO1 at the t20 minute timepoint


title: SMY2112_SFL1_t10

source_name: SMY2112 including SFL1 at the t10 minute timepoint


title: SMY2112_SFL1_t15

source_name: SMY2112 including SFL1 at the t15 minute timepoint


title: SMY2112_SFL1_t20

source_name: SMY2112 including SFL1 at the t20 minute timepoint


title: SMY2115_SKO1_t0

source_name: SMY2115 including SKO1 at the t0 minute timepoint


title: SMY2112_SFL1_t5

source_name: SMY2112 including SFL1 at the t5 minute timepoint


title: SMY2111_RTG3_t90

source_name: SMY2111 including RTG3 at the t90 minute timepoint


title: SMY2111_RTG3_t45

source_name: SMY2111 including RTG3 at the t45 minute timepoint


title: SMY2078_IME1_t20

source_name: SMY2078 including IME1 at the t20 minute timepoint


title: SMY2111_RTG3_t5

source_name: SMY2111 including RTG3 at the t5 minute timepoint


title: SMY2111_RTG3_t0

source_name: SMY2111 including RTG3 at the t0 minute timepoint


title: SMY2078_IME1_t15

source_name: SMY2078 including IME1 at the t15 minute timepoint


title: SMY2111_RTG3_t20

source_name: SMY2111 including RTG3 at the t20 minute timepoint


title: SMY2076_HAP5_t90

source_name: SMY2076 including HAP5 at the t90 minute timepoint


title: SMY2111_RTG3_t15

source_name: SMY2111 including RTG3 at the t15 minute timepoint


title: SMY2078_IME1_t0

source_name: SMY2078 including IME1 at the t0 minute timepoint


title: SMY2078_IME1_t5

source_name: SMY2078 including IME1 at the t5 minute timepoint


title: SMY2111_RTG3_t10

source_name: SMY2111 including RTG3 at the t10 minute timepoint


title: SMY2078_IME1_t45

source_name: SMY2078 including IME1 at the t45 minute timepoint


title: SMY2078_IME1_t10

source_name: SMY2078 including IME1 at the t10 minute timepoint


title: SMY2078_IME1_t30

source_name: SMY2078 including IME1 at the t30 minute timepoint


title: SMY2112_SFL1_t0

source_name: SMY2112 including SFL1 at the t0 minute timepoint


title: SMY2111_RTG3_t30

source_name: SMY2111 including RTG3 at the t30 minute timepoint


title: SMY2076_HAP5_t30

source_name: SMY2076 including HAP5 at the t30 minute timepoint


title: SMY2078_IME1_t90

source_name: SMY2078 including IME1 at the t90 minute timepoint


title: SMY2076_HAP5_t20

source_name: SMY2076 including HAP5 at the t20 minute timepoint


title: SMY2075_HAP4_t45

source_name: SMY2075 including HAP4 at the t45 minute timepoint


title: SMY2075_HAP4_t30

source_name: SMY2075 including HAP4 at the t30 minute timepoint


title: SMY2076_HAP5_t10

source_name: SMY2076 including HAP5 at the t10 minute timepoint


title: SMY2076_HAP5_t15

source_name: SMY2076 including HAP5 at the t15 minute timepoint


title: SMY2075_HAP4_t90

source_name: SMY2075 including HAP4 at the t90 minute timepoint


title: SMY2075_HAP4_t20

source_name: SMY2075 including HAP4 at the t20 minute timepoint


title: SMY2076_HAP5_t0

source_name: SMY2076 including HAP5 at the t0 minute timepoint


title: SMY2127_UME6_t90

source_name: SMY2127 including UME6 at the t90 minute timepoint


title: SMY2075_HAP4_t15

source_name: SMY2075 including HAP4 at the t15 minute timepoint


title: SMY2075_HAP4_t10

source_name: SMY2075 including HAP4 at the t10 minute timepoint


title: SMY2074_HAP3_t90

source_name: SMY2074 including HAP3 at the t90 minute timepoint


title: SMY2115_SKO1_t5

source_name: SMY2115 including SKO1 at the t5 minute timepoint


title: SMY2075_HAP4_t0

source_name: SMY2075 including HAP4 at the t0 minute timepoint


title: SMY2075_HAP4_t5

source_name: SMY2075 including HAP4 at the t5 minute timepoint


title: SMY2074_HAP3_t20

source_name: SMY2074 including HAP3 at the t20 minute timepoint


title: SMY2074_HAP3_t45

source_name: SMY2074 including HAP3 at the t45 minute timepoint


title: SMY2074_HAP3_t30

source_name: SMY2074 including HAP3 at the t30 minute timepoint


title: SMY2076_HAP5_t5

source_name: SMY2076 including HAP5 at the t5 minute timepoint


title: SMY2074_HAP3_t15

source_name: SMY2074 including HAP3 at the t15 minute timepoint


title: SMY2074_HAP3_t0

source_name: SMY2074 including HAP3 at the t0 minute timepoint


title: SMY2073_HAP2_t45

source_name: SMY2073 including HAP2 at the t45 minute timepoint


title: SMY2073_HAP2_t30

source_name: SMY2073 including HAP2 at the t30 minute timepoint


title: SMY2073_HAP2_t20

source_name: SMY2073 including HAP2 at the t20 minute timepoint


title: SMY2073_HAP2_t90

source_name: SMY2073 including HAP2 at the t90 minute timepoint


title: mTeSR1_matrigel_H9 [WAM528A20_S29]

type: SRA

city: Biberach an der Riss

first: Gregorio

last: Alanis-Lobato

address: Birkendorfer Str 65

department: Global Computational Biology and Digital Sciences

country: Germany

institute: Boehringer Ingelheim Pharma

description:
mTeSR1_matrigel_H9
hESC cultured in mTeSR1 medium

library_source: transcriptomic

data_processing:
Read quality control was performed using FastQC v0.11.6 and results integrated with multiqc v0.9. Quality and adapter trimming with Trim Galore! v0.5.0 were applied to samples that did not pass the quality controls (mainly due to adapter contamination).
Mapping was performed against the human reference transcriptome GRCh38 using salmon v0.11.3 to generate *_quant.sf files.
Transcript IDs were mapped to gene IDs and official gene symbols using biomaRt v2.36.1.
Gene-level summarisation of counts was carried out with tximport v1.8.0.
Genome_build: Human reference transcriptome GRCh38
Supplementary_files_format_and_content: Quantification files produced by salmon v0.11.3 (*.sf) and gene-level summarisation of counts from tximport v1.8.0 in R's list format.

molecule: polyA RNA

organism: Homo sapiens

source_name: Human embryonic stem cell

growth_protocol: For culture in mTeSR1 medium (StemCell Technologies), cells were generally maintained on Matrigel-coated (BD Biosciences) tissue culture plates. Matrigel coating was performed for one hour at room temperature (RT) per the manufacturer’s instructions. Cells were passaged as clumps using ReLeSR (Stemcell Technologies). ReLeSR was added to wells for 30 seconds at RT then aspirated, then plates were incubated for 5 minutes at 37°C, quenched with mTeSR1 and lightly tapped to dislodge clumps of the desired size. Cells were also adapted to plates coated with 0.5 μg/cm2 Laminin 511 (Biolamina LN-511, Takara iMatrix-511 T303). Laminin coating was performed either overnight at 4°C or for one hour at 37°C per manufacturer’s instructions. Cells on Laminin-511 were also passaged with ReLeSR with a 7-minute incubation at 37°C, or dissociated to single cell level with Accumax (Fisher Scientific), with a 10 minute incubation at 37°C. For culture in AI medium, cells were maintained on plates coated with 0.5 μg/cm2 Laminin 511. Early passage newly-derived hESCs in AI medium were passaged by manual picking. Established cells in AI medium were also split using various dissociation reagents. For ReLeSR, cells were passaged similar to cells in mTeSR1 on Laminin. For Accutase (StemCell Technologies) or AccuMax, dissociation reagent was added to wells for 5 – 10 minutes, the well contents were collected and pelleted, and the dissociation reagent removed. Cells were resuspended in AI medium with or without 10 μM ROCK inhibitor (Y27632, Tocris) for plating. For Gentle Cell Dissociation Reagent (StemCell Technologies), reagent was added to wells for 6 minutes, then aspirated and AI medium added. Well contents were collected and disaggregated with a 5 ml stripette. For 0.5 mM EDTA (Sigma), reagent was added for 5 minutes then aspirated, AI medium added and cells disaggregated. For salt-free PBS (Gibco, Life Technologies), reagent was added for 6 minutes, aspirated, then AI medium added and cells disaggregated.

extract_protocol:
cDNA was generated from single cells using the SMART-Seq V4 Ultra Low Input Kit (Clontech 634898) as previously described (Blakeley et al. 2015). cDNA was amplified by adding 25 μl 2X SeqAmp PCR buffer, 1 μl PCR Primer II A (12 μM), 1 μl SeqAmp DNA polymerase, 3 μl nuclease-free water, and incubated on a PCR machine at 95°C for 1 min, 23 cycles of 98°C for 10 s, 65°C for 30 s and 68°C for 3 min, before a final extension for 10 min at 72°C. Amplified cDNA was purified using 50 μl AMPure XP beads (Beckman Coulter) per manufacturer’s instructions. Sample tubes were mixed well and incubated at RT for 8 min to bind the DNA to the beads. Tubes were put on a magnet until the supernatant ran clear so that it could be removed and discarded. The beads were washed twice with 200 μl 80% ethanol. Any remaining ethanol was removed and beads allowed to dry, then resuspended in 12 μl of elution buffer. Tubes were returned to the magnet and the clear supernatant containing the cDNA was collected in a new tube and stored at −80°C until library preparation. cDNA quality was assessed by High Sensitivity DNA assay on an Agilent 2100 Bioanalyser, with good quality cDNA showing a broad peak from 300 to 9000 bp. cDNA concentration was measured using Qubit dsDNA HS kit (Life Technologies).
In preparation for library generation, 10 μl of cDNA sample and 32 μl purification buffer were added to a Covaris microTUBE AFA Fiber Pre-Slit Snap Cap. cDNA was sheared to a 200-500 bp range using an E220 focused-ultrasonicator (Covaris) for 2 mins at Peak Incident power 175 W, Duty Factor 10%, 200 cycles per burst, water level 5. Libraries were prepared using a Low Input Library Prep Kit HT (Clontech, 634900) per manufacturer’s instructions. Library quality and DNA concentration was assessed by Bioanalyser and Qubit. For library purification, 75 μl of AMPure XP beads was added to each collection tube containing the genomic DNA and treated as described above.

tag: cell type

value: pluripotent stem cell

tag: cell line

tag: growth condition


title: mTeSR1_matrigel_H9 [WAM528A19_S27]


title: mTeSR1_matrigel_H9 [WAM528A18_S26]


title: SMY2073_HAP2_t10

source_name: SMY2073 including HAP2 at the t10 minute timepoint


title: SMY2074_HAP3_t10

source_name: SMY2074 including HAP3 at the t10 minute timepoint


title: SMY2074_HAP3_t5

source_name: SMY2074 including HAP3 at the t5 minute timepoint


title: SMY2073_HAP2_t0

source_name: SMY2073 including HAP2 at the t0 minute timepoint


title: mTeSR1_laminin_H9 [WAM528A8_S35]

description:
mTeSR1_laminin_H9
hESC cultured in mTeSR1 medium over laminin-coated plates

value: mTeSR1 laminin


title: mTeSR1_matrigel_H9 [WAM528A16_S24]


title: mTeSR1_laminin_H9 [WAM528A9_S36]


title: SMY2071_GSM1_t45

source_name: SMY2071 including GSM1 at the t45 minute timepoint


title: mTeSR1_laminin_H1 [WAM528A5_S32]

description:
mTeSR1_laminin_H1
hESC cultured in mTeSR1 medium over laminin-coated plates


title: mTeSR1_laminin_H9 [WAM528A10_S18]


title: mTeSR1_matrigel_H9 [WAM528A17_S25]


title: mTeSR1_laminin_H9 [WAM528A7_S34]


title: mTeSR1_laminin_H1 [WAM528A4_S31]


title: mTeSR1_laminin_H9 [WAM528A6_S33]


title: SMY2071_GSM1_t20

source_name: SMY2071 including GSM1 at the t20 minute timepoint


title: SMY2071_GSM1_t10

source_name: SMY2071 including GSM1 at the t10 minute timepoint


title: SMY2071_GSM1_t5

source_name: SMY2071 including GSM1 at the t5 minute timepoint


title: mTeSR1_laminin_H1 [WAM528A2_S28]


title: SMY2067_GAL4_t45

source_name: SMY2067 including GAL4 at the t45 minute timepoint


title: mTeSR1_laminin_H1 [WAM528A3_S30]


title: mTeSR1_laminin_H1 [WAM528A1_S17]


title: SMY2073_HAP2_t5

source_name: SMY2073 including HAP2 at the t5 minute timepoint


title: SMY2067_GAL4_t30

source_name: SMY2067 including GAL4 at the t30 minute timepoint


title: SMY2071_GSM1_t15

source_name: SMY2071 including GSM1 at the t15 minute timepoint


title: SMY2071_GSM1_t90

source_name: SMY2071 including GSM1 at the t90 minute timepoint


title: SMY2067_GAL4_t90

source_name: SMY2067 including GAL4 at the t90 minute timepoint


title: SMY2071_GSM1_t30

source_name: SMY2071 including GSM1 at the t30 minute timepoint


title: SMY2071_GSM1_t0

source_name: SMY2071 including GSM1 at the t0 minute timepoint


title: AI_CH3 [WAM528A15_S23]

description:
AI_CH3
hESC cultured in Activin and IGF1 (AI) medium

source_name: Human embryonic stem cell

value: AI


title: SMY2067_GAL4_t20

source_name: SMY2067 including GAL4 at the t20 minute timepoint


title: SMY2067_GAL4_t15

source_name: SMY2067 including GAL4 at the t15 minute timepoint


title: AI_CH3 [WAM528A14_S22]


title: SMY2067_GAL4_t5

source_name: SMY2067 including GAL4 at the t5 minute timepoint