title: F8_V1_N4

description: Young (3 month old) treated with PBS


title: B1_V2_I6

status: Public on Mar 05 2020

type: SRA

city: Stanford

first: Michelle

middle: Berkeley

last: Chen

address: 318 Campus Drive

country: USA

institute: Stanford

description: Young (3 month old) treated with PBS

library_source: transcriptomic

data_processing:
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm8 whole genome using bowtie v0.12.2 with parameters -q -p 4 -e 100 -y -a -m 10 --best --strata
Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using a protocol from Chepelev et al., Nucleic Acids Research, 2009. In short, exons from all isoforms of a gene were merged to create one meta-transcript. The number of reads falling in the exons of this meta-transcript were counted and normalized by the size of the meta-transcript and by the size of the library.
Genome_build: mm10
Supplementary_files_format_and_content: raw count table, genes X cells

molecule: total RNA

organism: Mus musculus

extract_protocol:
Brain tissues were dissected using Miltenyi neural dissociation kit. Brain endothelial cells were isolated via FACS.
RNA libraries were prepared for sequencing using standard Illumina protocols

tag: cell type

value: primary murine brain endothelial cells


title: F8_V7_B15

description: Young (3 month old) treated with PBS


title: G10_V2_F9

description: Young (3 month old) treated with PBS


title: E4_V8_P18

description: Young (3 month old) treated with PBS


title: F4_V1_J4

description: Young (3 month old) treated with PBS


title: E7_V2_F8

description: Young (3 month old) treated with PBS


title: B11_V7_N12

description: Young (3 month old) treated with PBS


title: E7_V7_K14

description: Young (3 month old) treated with PBS


title: E4_V7_I14

description: Young (3 month old) treated with PBS


title: A5_V8_H16

description: Young (3 month old) treated with PBS


title: A5_V7_P11

description: Young (3 month old) treated with PBS


title: E4_V1_A4

description: Young (3 month old) treated with PBS


title: C5_V7_D13

description: Young (3 month old) treated with PBS


title: F11_V7_D15

description: Young (3 month old) treated with PBS


title: B10_V8_F17

description: Young (3 month old) treated with PBS


title: F11_V8_M19

description: Young (3 month old) treated with PBS


title: E2_V7_G14

description: Young (3 month old) treated with PBS


title: B10_V2_O6

description: Young (3 month old) treated with PBS


title: F11_V2_N8

description: Young (3 month old) treated with PBS


title: E2_V8_N18

description: Young (3 month old) treated with PBS


title: E1_V8_M18

description: Young (3 month old) treated with PBS


title: E1_V2_P7

description: Young (3 month old) treated with PBS


title: E2_V2_A8

description: Young (3 month old) treated with PBS


title: E1_V1_N3

description: Young (3 month old) treated with PBS


title: A7_V7_A12

description: Young (3 month old) treated with PBS


title: E3_V7_H14

description: Young (3 month old) treated with PBS


title: A7_V8_J16

description: Young (3 month old) treated with PBS


title: A4_V7_O11

description: Young (3 month old) treated with PBS


title: A11_V8_N16

description: Young (3 month old) treated with PBS


title: C5_V8_M17

description: Young (3 month old) treated with PBS


title: E3_V8_O18

description: Young (3 month old) treated with PBS


title: F10_V1_P4

description: Young (3 month old) treated with PBS


title: D1_V1_C3

description: Young (3 month old) treated with PBS


title: D1_V7_L13

description: Young (3 month old) treated with PBS


title: C1_V7_P12

description: Young (3 month old) treated with PBS


title: E1_W8_AMP_1_H21

description: Young (3 month old) treated with Aged (19 month old) plasma


title: F11_W2_AMP_1_H10


title: C1_V8_I17

description: Young (3 month old) treated with PBS


title: A11_V7_E12

description: Young (3 month old) treated with PBS


title: F11_Q3_AMP_C17


title: E1_A5_AMP_A4


title: E3_V1_P3

description: Young (3 month old) treated with PBS


title: F11_A5_AMP_G5


title: C1_V2_B7

description: Young (3 month old) treated with PBS


title: F10_V8_L19

description: Young (3 month old) treated with PBS


title: E1_V7_F14

description: Young (3 month old) treated with PBS


title: E1_W2_AMP_1_B9


title: C5_A5_AMP_M2


title: C5_W2_AMP_1_N7


title: F7_V1_M4

description: Young (3 month old) treated with PBS


title: D1_V8_B18

description: Young (3 month old) treated with PBS


title: C5_Q3_AMP_I14


title: G4_Q3_AMP_H17


title: G4_W8_AMP_1_C23


title: F7_W2_AMP_1_D10


title: F7_W8_AMP_1_J22


title: G4_W2_AMP_1_M10


title: F7_Q3_AMP_O16


title: H5_A5_AMP_I6


title: H5_Q3_AMP_E18


title: E6_A5_AMP_F4


title: G11_Q3_AMP_O17


title: G11_W8_AMP_1_J23


title: E6_W2_AMP_1_G9


title: H5_W8_AMP_1_P23


title: E9_W8_AMP_1_P21


title: E9_W2_AMP_1_J9


title: E6_Q3_AMP_B16


title: E9_A5_AMP_I4


title: G11_W2_AMP_1_D11


title: D10_W2_AMP_1_O8


title: E9_Q3_AMP_E16


title: D10_W8_AMP_1_E21


title: E6_W8_AMP_1_M21


title: D10_Q3_AMP_J15


title: D11_W8_AMP_1_F21


title: D11_A5_AMP_O3


title: G4_A5_AMP_L5


title: D11_Q3_AMP_K15


title: G11_A5_AMP_C6


title: E7_W2_AMP_1_H9


title: F9_W2_AMP_1_F10


title: E7_W8_AMP_1_N21


title: D11_W2_AMP_1_P8


title: F9_A5_AMP_E5


title: E10_W8_AMP_1_A22


title: E10_Q3_AMP_F16


title: E10_W2_AMP_1_K9


title: C6_W2_AMP_1_O7


title: C6_Q3_AMP_J14


title: G10_Q3_AMP_N17


title: G10_W2_AMP_1_C11


title: G8_Q3_AMP_L17


title: C11_W8_AMP_1_J20


title: D10_A5_AMP_N3


title: G10_W8_AMP_1_I23


title: H10_Q3_AMP_J18


title: C11_A5_AMP_C3


title: C11_W2_AMP_1_D8


title: E7_Q3_AMP_C16


title: H10_A5_AMP_N6


title: H10_W8_AMP_1_E24


title: H10_W2_AMP_1_N11


title: F9_Q3_AMP_A17


title: G6_W8_AMP_1_E23


title: G6_Q3_AMP_J17


title: F9_W8_AMP_1_L22


title: C6_A5_AMP_N2


title: G6_W2_AMP_1_O10


title: B12_W8_AMP_1_B20


title: G8_A5_AMP_P5


title: B12_A5_AMP_H2


title: G8_W2_AMP_1_A11


title: B12_Q3_AMP_D14


title: C9_W8_AMP_1_H20


title: C9_W2_AMP_1_B8


title: C9_A5_AMP_A3


title: C9_Q3_AMP_M14


title: G10_A5_AMP_B6


title: E2_V1_O3

description: Young (3 month old) treated with PBS


title: A9_Q3_AMP_E13


title: A10_Q3_AMP_F13


title: A9_A5_AMP_I1


title: A10_A5_AMP_J1


title: A10_W8_AMP_1_D19


title: A10_W2_AMP_1_K6


title: A9_W8_AMP_1_C19


title: E4_W2_AMP_1_E9


title: E4_A5_AMP_D4


title: H4_Q3_AMP_D18


title: E4_W8_AMP_1_K21


title: E4_Q3_AMP_P15


title: H4_W8_AMP_1_O23


title: H4_A5_AMP_H6


title: H4_W2_AMP_1_H11


title: H1_A5_AMP_E6


title: H1_W8_AMP_1_L23


title: G2_Q3_AMP_F17


title: G2_W8_AMP_1_A23


title: G2_W2_AMP_1_K10


title: A4_A5_AMP_D1


title: A4_W2_AMP_1_E6


title: A4_W8_AMP_1_N18


title: A4_Q3_AMP_P12


title: E5_W8_AMP_1_L21


title: G2_A5_AMP_J5


title: C10_W8_AMP_1_I20


title: C10_A5_AMP_B3


title: C10_W2_AMP_1_C8


title: D3_Q3_AMP_C15


title: D3_W8_AMP_1_N20


title: B3_Q3_AMP_K13


title: D3_W2_AMP_1_H8


title: B3_W8_AMP_1_I19


title: B3_W2_AMP_1_P6


title: B3_A5_AMP_O1


title: F2_W2_AMP_1_O9


title: F2_A5_AMP_N4


title: F1_W8_AMP_1_D22


title: D8_Q3_AMP_H15


title: E5_A5_AMP_E4


title: F2_W8_AMP_1_E22


title: F1_Q3_AMP_I16


title: E5_W2_AMP_1_F9


title: F1_A5_AMP_M4


title: F1_W2_AMP_1_N9


title: G1_W2_AMP_1_J10


title: G5_W2_AMP_1_N10


title: G1_W8_AMP_1_P22


title: G5_A5_AMP_M5


title: E8_W8_AMP_1_O21


title: E8_W2_AMP_1_I9


title: G5_W8_AMP_1_D23


title: D8_A5_AMP_L3


title: A7_Q3_AMP_C13


title: D9_W2_AMP_1_N8


title: D9_A5_AMP_M3


title: E8_A5_AMP_H4


title: A7_W8_AMP_1_A19


title: A7_A5_AMP_G1


title: C2_A5_AMP_J2


title: A3_W2_AMP_1_D6


title: A3_A5_AMP_C1


title: C2_Q3_AMP_F14


title: A3_Q3_AMP_O12


title: C2_W2_AMP_1_K7


title: G1_A5_AMP_I5


title: A3_W8_AMP_1_M18


title: H9_Q3_AMP_I18


title: G5_Q3_AMP_I17


title: H9_W2_AMP_1_M11


title: H9_W8_AMP_1_D24


title: G9_W8_AMP_1_H23


title: G9_Q3_AMP_M17


title: G9_W2_AMP_1_B11


title: B6_A5_AMP_B2


title: B6_Q3_AMP_N13


title: D4_W2_AMP_1_I8


title: D9_Q3_AMP_I15


title: D4_Q3_AMP_D15


title: D4_W8_AMP_1_O20


title: D9_W8_AMP_1_D21


title: D7_A5_AMP_K3


title: B6_W2_AMP_1_C7


title: D4_A5_AMP_H3


title: H5_W2_AMP_1_I11


title: A6_W2_AMP_1_G6


title: D7_Q3_AMP_G15


title: A6_W8_AMP_1_P18


title: B2_W8_AMP_1_H19


title: B2_Q3_AMP_J13


title: D7_W8_AMP_1_B21


title: A6_Q3_AMP_B13


title: A6_A5_AMP_F1


title: B2_A5_AMP_N1


title: B2_W2_AMP_1_O6


title: G9_A5_AMP_A6


title: H7_W8_AMP_1_B24


title: H7_W2_AMP_1_K11


title: F8_A5_AMP_D5


title: F8_W2_AMP_1_E10


title: F8_Q3_AMP_P16


title: F10_Q3_AMP_B17


title: F8_W8_AMP_1_K22


title: D7_W2_AMP_1_L8


title: B7_Q3_AMP_O13


title: B7_W2_AMP_1_D7


title: B7_A5_AMP_C2


title: B8_Q3_AMP_P13


title: B7_W8_AMP_1_M19


title: B8_W2_AMP_1_E7


title: B8_W8_AMP_1_N19


title: F10_A5_AMP_F5


title: A8_A5_AMP_H1


title: A8_Q3_AMP_D13


title: A8_W2_AMP_1_I6


title: A8_W8_AMP_1_B19


title: F10_W8_AMP_1_M22


title: H7_A5_AMP_K6


title: C8_Q3_AMP_L14


title: H3_W8_AMP_1_N23


title: C8_W8_AMP_1_G20


title: C8_A5_AMP_P2


title: H3_Q3_AMP_C18


title: H7_Q3_AMP_G18


title: H3_A5_AMP_G6


title: H3_W2_AMP_1_G11


title: G3_A5_AMP_K5


title: B8_A5_AMP_D2


title: G3_W2_AMP_1_L10


title: G3_W8_AMP_1_B23


title: G3_Q3_AMP_G17


title: C7_Q3_AMP_K14


title: F5_Q3_AMP_M16


title: F5_A5_AMP_A5


title: F3_W2_AMP_1_P9


title: C1_Q3_AMP_E14


title: F5_W8_AMP_1_H22


title: C1_A5_AMP_I2


title: C7_W2_AMP_1_P7


title: F5_W2_AMP_1_B10


title: F3_W8_AMP_1_F22


title: F3_Q3_AMP_K16


title: F3_A5_AMP_O4


title: B10_Q3_AMP_B14


title: B10_W2_AMP_1_G7


title: D12_Q3_AMP_L15


title: D12_W2_AMP_1_A9


title: D12_W8_AMP_1_G21


title: D12_A5_AMP_P3


title: C7_A5_AMP_O2


title: A11_Q3_AMP_G13


title: A11_W8_AMP_1_E19


title: A11_A5_AMP_K1


title: D2_W8_AMP_1_M20


title: D2_Q3_AMP_B15


title: D2_A5_AMP_F3


title: H11_W2_AMP_1_O11


title: A11_W2_AMP_1_L6


title: H11_W8_AMP_1_F24


title: H2_W8_AMP_1_M23


title: H11_Q3_AMP_K18


title: H2_Q3_AMP_B18


title: H2_W2_AMP_1_F11


title: A1_W2_AMP_1_B6


title: H2_A5_AMP_F6


title: B10_W8_AMP_1_P19


title: A1_A5_AMP_A1


title: G8_W8_AMP_1_G23


title: H11_A5_AMP_O6


title: D6_W8_AMP_1_A21


title: D6_A5_AMP_J3


title: A1_W8_AMP_1_K18


title: F4_Q3_AMP_L16


title: F4_A5_AMP_P4


title: D6_W2_AMP_1_K8


title: E2_W2_AMP_1_C9


title: E2_A5_AMP_B4


title: D3_A5_AMP_G3


title: E2_Q3_AMP_N15


title: B5_Q3_AMP_M13


title: E2_W8_AMP_1_I21


title: B5_W2_AMP_1_B7


title: B5_A5_AMP_A2


title: C3_Q3_AMP_G14


title: A5_W2_AMP_1_F6


title: B9_A5_AMP_E2


title: A5_A5_AMP_E1


title: C3_W8_AMP_1_E20


title: B5_W8_AMP_1_K19


title: A5_Q3_AMP_A13


title: B9_Q3_AMP_A14


title: B1_W8_AMP_1_G19


title: B1_Q3_AMP_I13


title: B9_W8_AMP_1_O19


title: B1_A5_AMP_M1


title: F4_W2_AMP_1_A10


title: B1_W2_AMP_1_N6


title: F6_A5_AMP_B5


title: A1_Q3_AMP_M12


title: F6_Q3_AMP_N16


title: F6_W2_AMP_1_C10


title: F6_W8_AMP_1_I22


title: A2_Q3_AMP_N12


title: A5_W8_AMP_1_O18


title: A2_A5_AMP_B1


title: A2_W2_AMP_1_C6


title: A2_W8_AMP_1_L18


title: G7_W8_AMP_1_F23


title: B9_W2_AMP_1_F7


title: G7_W2_AMP_1_P10


title: G7_A5_AMP_O5


title: G7_Q3_AMP_K17


title: D1_Q3_AMP_A15


title: E3_A5_AMP_C4


title: D1_A5_AMP_E3


title: D1_W8_AMP_1_L20


title: D1_W2_AMP_1_F8


title: C12_Q3_AMP_P14


title: E3_W2_AMP_1_D9


title: C12_W2_AMP_1_E8


title: E3_W8_AMP_1_J21


title: C12_A5_AMP_D3


title: A12_W8_AMP_1_F19


title: G1_Q3_AMP_E17


title: A12_Q3_AMP_H13


title: A12_A5_AMP_L1


title: E11_W2_AMP_1_L9


title: A12_W2_AMP_1_M6


title: H8_Q3_AMP_H18


title: E11_Q3_AMP_G16


title: E11_W8_AMP_1_B22


title: H8_W8_AMP_1_C24


title: H8_A5_AMP_L6


title: B4_W8_AMP_1_J19


title: B4_W2_AMP_1_A7


title: B11_W8_AMP_1_A20


title: C4_W8_AMP_1_F20


title: B4_Q3_AMP_L13


title: C4_W2_AMP_1_M7


title: B11_A5_AMP_G2


title: B11_Q3_AMP_C14


title: H6_Q3_AMP_F18


title: H6_W8_AMP_1_A24


title: C4_A5_AMP_L2


title: H6_W2_AMP_1_J11


title: C4_Q3_AMP_H14


title: D5_Q3_AMP_E15


title: C12_W8_AMP_1_K20


title: D5_W2_AMP_1_J8


title: H8_W2_AMP_1_L11


title: m2_Aged_N10_new_384

description: Aged (19 month old)


title: D5_A5_AMP_I3


title: m2_Young_E10_new_384

description: Young (3 month old)


title: m2_Young_O7_new_384

description: Young (3 month old)


title: m2_Young_A19_new_384

description: Young (3 month old)


title: m2_Young_O8_new_384

description: Young (3 month old)


title: m2_Aged_I15_new_384

description: Aged (19 month old)


title: D5_W8_AMP_1_P20


title: m2_Aged_A20_new_384

description: Aged (19 month old)


title: m2_Aged_C17_new_384

description: Aged (19 month old)


title: m2_Aged_H15_new_384

description: Aged (19 month old)


title: B10_A5_AMP_F2


title: H6_A5_AMP_J6


title: m2_Aged_F8_new_384

description: Aged (19 month old)


title: m2_Aged_C16_new_384

description: Aged (19 month old)


title: m2_Young_F20_new_384

description: Young (3 month old)


title: m2_Young_O4_new_384

description: Young (3 month old)


title: m2_Young_B23_new_384

description: Young (3 month old)


title: m2_Aged_P10_new_384

description: Aged (19 month old)


title: m2_Aged_E9_new_384

description: Aged (19 month old)


title: m2_Aged_J15_new_384

description: Aged (19 month old)


title: B4_A5_AMP_P1


title: m2_Young_B22_new_384

description: Young (3 month old)


title: m2_Aged_C23_new_384

description: Aged (19 month old)


title: m2_Young_L15_new_384

description: Young (3 month old)


title: m2_Aged_J21_new_384

description: Aged (19 month old)


title: m2_Young_G15_new_384

description: Young (3 month old)


title: m2_Young_D22_new_384

description: Young (3 month old)


title: m2_Young_F17_new_384

description: Young (3 month old)


title: D6_Q3_AMP_F15


title: m2_Aged_J14_new_384

description: Aged (19 month old)


title: m2_Young_H15_new_384

description: Young (3 month old)


title: m2_Young_C6_new_384

description: Young (3 month old)


title: m2_Young_O21_new_384

description: Young (3 month old)


title: m2_Young_I4_new_384

description: Young (3 month old)


title: m2_Aged_H11_new_384

description: Aged (19 month old)


title: m2_Young_A15_new_384

description: Young (3 month old)


title: m2_Aged_N18_new_384

description: Aged (19 month old)


title: m2_Aged_K22_new_384

description: Aged (19 month old)


title: m2_Aged_G6_new_384

description: Aged (19 month old)


title: m2_Young_K20_new_384

description: Young (3 month old)


title: m2_Aged_J16_new_384

description: Aged (19 month old)


title: m2_Aged_D22_new_384

description: Aged (19 month old)


title: m2_Aged_L16_new_384

description: Aged (19 month old)


title: m2_Aged_O14_new_384

description: Aged (19 month old)


title: m2_Aged_C7_new_384

description: Aged (19 month old)


title: m2_Young_G14_new_384

description: Young (3 month old)


title: m2_Aged_G7_new_384

description: Aged (19 month old)


title: m2_Young_O23_new_384

description: Young (3 month old)


title: m2_Young_L21_new_384

description: Young (3 month old)


title: m2_Young_I15_new_384

description: Young (3 month old)


title: m2_Young_E13_new_384

description: Young (3 month old)


title: m2_Young_L14_new_384

description: Young (3 month old)


title: m2_Young_P13_new_384

description: Young (3 month old)


title: m2_Aged_E3_new_384

description: Aged (19 month old)


title: m2_Aged_E17_new_384

description: Aged (19 month old)


title: m2_Young_N13_new_384

description: Young (3 month old)


title: m2_Aged_B15_new_384

description: Aged (19 month old)


title: m2_Young_J16_new_384

description: Young (3 month old)


title: m2_Aged_O10_new_384

description: Aged (19 month old)


title: m2_Young_A7_new_384

description: Young (3 month old)


title: m2_Aged_D20_new_384

description: Aged (19 month old)


title: m2_Young_L23_new_384

description: Young (3 month old)


title: m2_Young_L18_new_384

description: Young (3 month old)


title: m2_Young_E14_new_384

description: Young (3 month old)


title: m2_Young_I22_new_384

description: Young (3 month old)


title: m2_Young_G9_new_384

description: Young (3 month old)


title: m2_Aged_N21_new_384

description: Aged (19 month old)


title: m2_Aged_D8_new_384

description: Aged (19 month old)


title: m2_Aged_K6_new_384

description: Aged (19 month old)


title: m2_Aged_K12_new_384

description: Aged (19 month old)


title: m2_Young_B9_new_384

description: Young (3 month old)


title: m2_Young_H21_new_384

description: Young (3 month old)


title: m2_Young_G22_new_384

description: Young (3 month old)


title: m2_Young_N12_new_384

description: Young (3 month old)


title: m2_Young_O5_new_384

description: Young (3 month old)


title: m2_Aged_J4_new_384

description: Aged (19 month old)


title: m2_Young_C19_new_384

description: Young (3 month old)


title: m2_Aged_O17_new_384

description: Aged (19 month old)


title: m2_Aged_L12_new_384

description: Aged (19 month old)


title: m2_Young_N10_new_384

description: Young (3 month old)


title: m2_Young_O6_new_384

description: Young (3 month old)


title: m2_Aged_E12_new_384

description: Aged (19 month old)


title: m2_Young_A6_new_384

description: Young (3 month old)


title: m2_Aged_C9_new_384

description: Aged (19 month old)


title: m2_Aged_L11_new_384

description: Aged (19 month old)


title: m2_Young_D10_new_384

description: Young (3 month old)


title: m2_Young_G16_new_384

description: Young (3 month old)


title: m2_Aged_O13_new_384

description: Aged (19 month old)


title: m2_Young_G21_new_384

description: Young (3 month old)


title: m2_Young_K19_new_384

description: Young (3 month old)


title: m2_Young_O10_new_384

description: Young (3 month old)


title: m2_Young_K11_new_384

description: Young (3 month old)


title: m2_Aged_I7_new_384

description: Aged (19 month old)


title: m2_Aged_O21_new_384

description: Aged (19 month old)


title: m2_Aged_B13_new_384

description: Aged (19 month old)


title: m2_Aged_K8_new_384

description: Aged (19 month old)


title: m2_Aged_L13_new_384

description: Aged (19 month old)


title: m2_Aged_O5_new_384

description: Aged (19 month old)


title: m2_Aged_P6_new_384

description: Aged (19 month old)


title: m2_Young_I11_new_384

description: Young (3 month old)


title: m2_Young_C10_new_384

description: Young (3 month old)


title: m2_Aged_D14_new_384

description: Aged (19 month old)


title: m2_Young_I8_new_384

description: Young (3 month old)


title: m2_Aged_I18_new_384

description: Aged (19 month old)


title: m2_Young_J8_new_384

description: Young (3 month old)


title: m2_Young_G4_new_384

description: Young (3 month old)


title: m2_Young_M21_new_384

description: Young (3 month old)


title: m2_Aged_C14_new_384

description: Aged (19 month old)


title: m2_Young_I18_new_384

description: Young (3 month old)


title: m2_Young_K17_new_384

description: Young (3 month old)


title: m2_Young_B15_new_384

description: Young (3 month old)


title: m2_Young_C13_new_384

description: Young (3 month old)


title: m2_Aged_D16_new_384

description: Aged (19 month old)


title: m2_Young_G17_new_384

description: Young (3 month old)


title: m2_Aged_M4_new_384

description: Aged (19 month old)


title: m2_Aged_K14_new_384

description: Aged (19 month old)


title: m2_Young_I20_new_384

description: Young (3 month old)


title: m2_Young_D23_new_384

description: Young (3 month old)


title: m2_Young_G19_new_384

description: Young (3 month old)


title: m2_Aged_C15_new_384

description: Aged (19 month old)


title: m2_Aged_H8_new_384

description: Aged (19 month old)


title: m2_Young_K16_new_384

description: Young (3 month old)


title: m2_Young_N22_new_384

description: Young (3 month old)


title: m2_Young_K22_new_384

description: Young (3 month old)


title: m2_Aged_N14_new_384

description: Aged (19 month old)


title: m2_Aged_L15_new_384

description: Aged (19 month old)


title: m2_Aged_N8_new_384

description: Aged (19 month old)


title: m2_Young_O15_new_384

description: Young (3 month old)


title: m2_Young_H18_new_384

description: Young (3 month old)


title: m2_Aged_B12_new_384

description: Aged (19 month old)


title: m2_Young_E22_new_384

description: Young (3 month old)


title: m2_Young_D20_new_384

description: Young (3 month old)


title: m2_Aged_D7_new_384

description: Aged (19 month old)


title: m2_Young_J10_new_384

description: Young (3 month old)


title: m2_Aged_B20_new_384

description: Aged (19 month old)


title: m2_Aged_J11_new_384

description: Aged (19 month old)


title: m2_Young_I12_new_384

description: Young (3 month old)


title: m2_Young_K6_new_384

description: Young (3 month old)


title: m2_Young_F21_new_384

description: Young (3 month old)


title: m2_Young_N18_new_384

description: Young (3 month old)


title: m2_Aged_B14_new_384

description: Aged (19 month old)


title: m2_Aged_N22_new_384

description: Aged (19 month old)


title: m2_Young_K23_new_384

description: Young (3 month old)


title: m2_Young_I19_new_384

description: Young (3 month old)


title: m2_Aged_A18_new_384

description: Aged (19 month old)


title: m2_Young_B17_new_384

description: Young (3 month old)


title: m2_Young_J11_new_384

description: Young (3 month old)


title: m2_Young_B19_new_384

description: Young (3 month old)


title: m2_Aged_A13_new_384

description: Aged (19 month old)


title: m2_Young_L20_new_384

description: Young (3 month old)


title: m2_Young_C12_new_384

description: Young (3 month old)


title: m2_Young_B12_new_384

description: Young (3 month old)


title: m2_Young_B20_new_384

description: Young (3 month old)


title: m2_Young_J21_new_384

description: Young (3 month old)


title: m2_Young_N14_new_384

description: Young (3 month old)


title: m2_Aged_K10_new_384

description: Aged (19 month old)


title: m2_Young_C16_new_384

description: Young (3 month old)


title: m2_Aged_N11_new_384

description: Aged (19 month old)


title: m2_Aged_G11_new_384

description: Aged (19 month old)


title: m2_Young_G10_new_384

description: Young (3 month old)


title: m2_Aged_A17_new_384

description: Aged (19 month old)


title: m2_Young_F22_new_384

description: Young (3 month old)


title: m2_Young_F11_new_384

description: Young (3 month old)


title: m2_Young_H20_new_384

description: Young (3 month old)


title: m2_Young_H17_new_384

description: Young (3 month old)


title: m2_Young_O19_new_384

description: Young (3 month old)


title: m2_Young_A13_new_384

description: Young (3 month old)


title: m2_Young_C22_new_384

description: Young (3 month old)


title: m2_Young_E19_new_384

description: Young (3 month old)


title: m2_Young_J22_new_384

description: Young (3 month old)


title: m2_Aged_B5_new_384

description: Aged (19 month old)


title: m2_Young_B14_new_384

description: Young (3 month old)


title: m2_Young_E23_new_384

description: Young (3 month old)


title: m2_Young_F18_new_384

description: Young (3 month old)


title: m2_Young_J6_new_384

description: Young (3 month old)


title: m2_Young_L17_new_384

description: Young (3 month old)


title: m2_Young_D11_new_384

description: Young (3 month old)


title: m2_Young_L16_new_384

description: Young (3 month old)


title: m2_Aged_E22_new_384

description: Aged (19 month old)


title: m2_Aged_H9_new_384

description: Aged (19 month old)


title: m2_Young_N8_new_384

description: Young (3 month old)


title: m2_Aged_F14_new_384

description: Aged (19 month old)


title: m2_Young_C7_new_384

description: Young (3 month old)


title: m2_Young_A16_new_384

description: Young (3 month old)


title: m2_Young_N21_new_384

description: Young (3 month old)


title: m2_Young_A18_new_384

description: Young (3 month old)


title: m2_Young_J17_new_384

description: Young (3 month old)


title: m2_Aged_L19_new_384

description: Aged (19 month old)


title: m2_Aged_J5_new_384

description: Aged (19 month old)


title: m2_Aged_J10_new_384

description: Aged (19 month old)


title: m2_Aged_A12_new_384

description: Aged (19 month old)


title: m2_Young_A9_new_384

description: Young (3 month old)


title: m2_Young_K21_new_384

description: Young (3 month old)


title: m2_Aged_B6_new_384

description: Aged (19 month old)


title: m2_Young_D21_new_384

description: Young (3 month old)


title: m2_Young_G13_new_384

description: Young (3 month old)


title: m2_Young_C8_new_384

description: Young (3 month old)


title: m2_Aged_D19_new_384

description: Aged (19 month old)


title: m2_Young_G11_new_384

description: Young (3 month old)


title: m2_Young_H16_new_384

description: Young (3 month old)


title: m2_Young_I23_new_384

description: Young (3 month old)


title: m2_Young_J20_new_384

description: Young (3 month old)


title: m2_Aged_C10_new_384

description: Aged (19 month old)


title: m2_Aged_H22_new_384

description: Aged (19 month old)


title: m2_Aged_K17_new_384

description: Aged (19 month old)


title: m2_Aged_C8_new_384

description: Aged (19 month old)


title: m2_Aged_E8_new_384

description: Aged (19 month old)


title: m2_Young_N19_new_384

description: Young (3 month old)


title: m2_Young_J13_new_384

description: Young (3 month old)


title: m2_Young_A8_new_384

description: Young (3 month old)


title: m2_Aged_I12_new_384

description: Aged (19 month old)


title: m2_Young_F6_new_384

description: Young (3 month old)


title: m2_Young_P19_new_384

description: Young (3 month old)


title: m2_Aged_G4_new_384

description: Aged (19 month old)


title: m2_Young_B21_new_384

description: Young (3 month old)


title: m2_Young_M6_new_384

description: Young (3 month old)


title: m2_Aged_A21_new_384

description: Aged (19 month old)


title: m2_Aged_M5_new_384

description: Aged (19 month old)


title: m2_Young_F23_new_384

description: Young (3 month old)


title: m2_Aged_D18_new_384

description: Aged (19 month old)


title: m2_Young_E9_new_384

description: Young (3 month old)


title: m2_Young_D17_new_384

description: Young (3 month old)


title: m2_Aged_F22_new_384

description: Aged (19 month old)


title: m2_Young_M3_new_384

description: Young (3 month old)


title: m2_Young_F14_new_384

description: Young (3 month old)


title: m2_Young_M23_new_384

description: Young (3 month old)


title: m2_Young_C21_new_384

description: Young (3 month old)


title: m2_Aged_H7_new_384

description: Aged (19 month old)


title: m2_Aged_J7_new_384

description: Aged (19 month old)


title: m2_Aged_L17_new_384

description: Aged (19 month old)


title: m2_Young_L9_new_384

description: Young (3 month old)


title: m2_Aged_E13_new_384

description: Aged (19 month old)


title: m2_Young_F7_new_384

description: Young (3 month old)


title: m2_Young_L10_new_384

description: Young (3 month old)


title: m2_Aged_K5_new_384

description: Aged (19 month old)


title: m2_Young_L5_new_384

description: Young (3 month old)


title: m2_Young_P5_new_384

description: Young (3 month old)


title: m2_Aged_H20_new_384

description: Aged (19 month old)


title: m2_Young_P20_new_384

description: Young (3 month old)


title: m2_Aged_H17_new_384

description: Aged (19 month old)


title: m2_Aged_G16_new_384

description: Aged (19 month old)


title: m2_Aged_J18_new_384

description: Aged (19 month old)


title: m2_Aged_E4_new_384

description: Aged (19 month old)


title: m2_Young_L6_new_384

description: Young (3 month old)


title: m2_Young_H10_new_384

description: Young (3 month old)


title: m2_Young_J12_new_384

description: Young (3 month old)


title: m2_Aged_I6_new_384

description: Aged (19 month old)


title: m2_Young_D7_new_384

description: Young (3 month old)


title: m2_Young_A21_new_384

description: Young (3 month old)


title: m2_Aged_K16_new_384

description: Aged (19 month old)


title: m2_Young_O20_new_384

description: Young (3 month old)


title: m2_Young_C23_new_384

description: Young (3 month old)


title: m2_Aged_K23_new_384

description: Aged (19 month old)


title: m2_Young_E5_new_384

description: Young (3 month old)


title: m2_Aged_E20_new_384

description: Aged (19 month old)


title: m2_Young_F12_new_384

description: Young (3 month old)


title: m2_Aged_P13_new_384

description: Aged (19 month old)


title: m2_Aged_A19_new_384

description: Aged (19 month old)


title: m2_Aged_B9_new_384

description: Aged (19 month old)


title: m2_Aged_J6_new_384

description: Aged (19 month old)


title: m2_Young_I17_new_384

description: Young (3 month old)


title: m2_Young_O13_new_384

description: Young (3 month old)


title: m2_Aged_I21_new_384

description: Aged (19 month old)


title: m2_Aged_C22_new_384

description: Aged (19 month old)


title: m2_Aged_F11_new_384

description: Aged (19 month old)


title: m2_Aged_L6_new_384

description: Aged (19 month old)


title: m2_Aged_I8_new_384

description: Aged (19 month old)


title: m2_Young_N9_new_384

description: Young (3 month old)


title: m2_Aged_N4_new_384

description: Aged (19 month old)


title: m2_Young_P21_new_384

description: Young (3 month old)


title: m2_Aged_K19_new_384

description: Aged (19 month old)


title: m2_Young_L4_new_384

description: Young (3 month old)


title: m2_Young_D5_new_384

description: Young (3 month old)


title: m2_Aged_P12_new_384

description: Aged (19 month old)


title: m2_Aged_L20_new_384

description: Aged (19 month old)


title: m2_Young_B7_new_384

description: Young (3 month old)


title: m2_Aged_F18_new_384

description: Aged (19 month old)


title: m2_Aged_B17_new_384

description: Aged (19 month old)


title: m2_Young_M5_new_384

description: Young (3 month old)


title: m2_Aged_L3_new_384

description: Aged (19 month old)


title: m2_Aged_J20_new_384

description: Aged (19 month old)


title: m2_Young_G6_new_384

description: Young (3 month old)


title: m2_Young_F4_new_384

description: Young (3 month old)


title: m2_Young_O14_new_384

description: Young (3 month old)


title: m2_Aged_H16_new_384

description: Aged (19 month old)


title: m2_Young_J5_new_384

description: Young (3 month old)


title: m2_Young_L3_new_384

description: Young (3 month old)


title: m2_Young_P15_new_384

description: Young (3 month old)


title: m2_Aged_F23_new_384

description: Aged (19 month old)


title: m2_Young_D19_new_384

description: Young (3 month old)


title: m2_Aged_F5_new_384

description: Aged (19 month old)


title: m2_Aged_D13_new_384

description: Aged (19 month old)


title: m2_Aged_A11_new_384

description: Aged (19 month old)


title: m2_Aged_G5_new_384

description: Aged (19 month old)


title: m2_Aged_G8_new_384

description: Aged (19 month old)


title: m2_Aged_L8_new_384

description: Aged (19 month old)


title: m2_Young_C11_new_384

description: Young (3 month old)


title: m2_Young_D6_new_384

description: Young (3 month old)


title: m2_Aged_I4_new_384

description: Aged (19 month old)


title: m2_Young_I5_new_384

description: Young (3 month old)


title: m2_Aged_J19_new_384

description: Aged (19 month old)


title: m2_Aged_L22_new_384

description: Aged (19 month old)


title: m2_Aged_P20_new_384

description: Aged (19 month old)


title: m2_Young_F5_new_384

description: Young (3 month old)


title: m2_Young_E18_new_384

description: Young (3 month old)


title: m2_Young_C20_new_384

description: Young (3 month old)


title: m2_Aged_J8_new_384

description: Aged (19 month old)


title: m2_Young_H23_new_384

description: Young (3 month old)


title: m2_Young_P7_new_384

description: Young (3 month old)


title: m2_Young_K8_new_384

description: Young (3 month old)


title: m2_Aged_O22_new_384

description: Aged (19 month old)


title: m2_Aged_J22_new_384

description: Aged (19 month old)


title: m2_Young_K18_new_384

description: Young (3 month old)


title: m2_Aged_P19_new_384

description: Aged (19 month old)


title: m2_Young_P12_new_384

description: Young (3 month old)


title: m2_Young_E20_new_384

description: Young (3 month old)


title: m2_Aged_O19_new_384

description: Aged (19 month old)


title: m2_Aged_M7_new_384

description: Aged (19 month old)


title: m2_Aged_M20_new_384

description: Aged (19 month old)


title: m2_Young_D15_new_384

description: Young (3 month old)


title: m2_Aged_K18_new_384

description: Aged (19 month old)


title: m2_Aged_J12_new_384

description: Aged (19 month old)


title: m2_Aged_E7_new_384

description: Aged (19 month old)


title: m2_Young_K7_new_384

description: Young (3 month old)


title: m2_Young_E21_new_384

description: Young (3 month old)


title: m2_Aged_L7_new_384

description: Aged (19 month old)


title: m2_Young_N11_new_384

description: Young (3 month old)


title: m2_Aged_D5_new_384

description: Aged (19 month old)


title: m2_Aged_K7_new_384

description: Aged (19 month old)


title: m2_Young_H6_new_384

description: Young (3 month old)


title: m2_Aged_O8_new_384

description: Aged (19 month old)


title: m2_Young_D4_new_384

description: Young (3 month old)


title: m2_Young_E11_new_384

description: Young (3 month old)


title: m2_Young_P16_new_384

description: Young (3 month old)


title: m2_Aged_N15_new_384

description: Aged (19 month old)


title: m2_Young_A22_new_384

description: Young (3 month old)


title: m2_Aged_O12_new_384

description: Aged (19 month old)


title: m2_Aged_I14_new_384

description: Aged (19 month old)


title: m2_Young_G23_new_384

description: Young (3 month old)


title: m2_Aged_O6_new_384

description: Aged (19 month old)


title: m2_Aged_O4_new_384

description: Aged (19 month old)


title: m2_Young_D13_new_384

description: Young (3 month old)


title: m2_Aged_G18_new_384

description: Aged (19 month old)


title: m2_Aged_L18_new_384

description: Aged (19 month old)


title: m2_Aged_F15_new_384

description: Aged (19 month old)


title: m2_Aged_B19_new_384

description: Aged (19 month old)


title: m2_Young_L22_new_384

description: Young (3 month old)


title: m2_Young_A12_new_384

description: Young (3 month old)


title: m2_Aged_H14_new_384

description: Aged (19 month old)


title: m2_Young_F3_new_384

description: Young (3 month old)


title: m2_Young_H5_new_384

description: Young (3 month old)


title: m2_Aged_J17_new_384

description: Aged (19 month old)


title: m2_Aged_N17_new_384

description: Aged (19 month old)


title: m2_Aged_A4_new_384

description: Aged (19 month old)


title: m2_Aged_I23_new_384

description: Aged (19 month old)


title: m2_Young_E8_new_384

description: Young (3 month old)


title: m2_Young_L19_new_384

description: Young (3 month old)


title: m2_Young_O18_new_384

description: Young (3 month old)


title: m2_Young_E7_new_384

description: Young (3 month old)


title: m2_Young_K13_new_384

description: Young (3 month old)


title: m2_Young_E16_new_384

description: Young (3 month old)


title: m2_Aged_D12_new_384

description: Aged (19 month old)


title: m2_Young_F13_new_384

description: Young (3 month old)


title: m2_Young_C4_new_384

description: Young (3 month old)


title: m2_Young_N23_new_384

description: Young (3 month old)


title: m2_Young_A11_new_384

description: Young (3 month old)


title: m2_Young_I6_new_384

description: Young (3 month old)


title: m2_Young_F16_new_384

description: Young (3 month old)


title: m2_Young_I7_new_384

description: Young (3 month old)


title: m2_Young_B5_new_384

description: Young (3 month old)


title: m2_Aged_O15_new_384

description: Aged (19 month old)


title: m2_Aged_F13_new_384

description: Aged (19 month old)


title: m2_Young_G20_new_384

description: Young (3 month old)


title: m2_Young_F8_new_384

description: Young (3 month old)


title: m2_Aged_G14_new_384

description: Aged (19 month old)


title: m2_Young_K10_new_384

description: Young (3 month old)


title: m2_Aged_G22_new_384

description: Aged (19 month old)


title: m2_Aged_N6_new_384

description: Aged (19 month old)


title: m2_Young_M22_new_384

description: Young (3 month old)


title: m2_Young_D8_new_384

description: Young (3 month old)


title: m2_Young_N16_new_384

description: Young (3 month old)


title: m2_Aged_H13_new_384

description: Aged (19 month old)


title: m2_Aged_F12_new_384

description: Aged (19 month old)


title: m2_Aged_E6_new_384

description: Aged (19 month old)


title: m2_Aged_P18_new_384

description: Aged (19 month old)


title: m2_Young_E3_new_384

description: Young (3 month old)


title: m2_Young_A14_new_384

description: Young (3 month old)


title: m2_Aged_K9_new_384

description: Aged (19 month old)


title: m2_Young_A23_new_384

description: Young (3 month old)


title: m2_Aged_O9_new_384

description: Aged (19 month old)


title: m2_Aged_N3_new_384

description: Aged (19 month old)


title: m2_Young_J4_new_384

description: Young (3 month old)


title: m2_Young_P11_new_384

description: Young (3 month old)


title: m2_Aged_J3_new_384

description: Aged (19 month old)


title: m2_Aged_H6_new_384

description: Aged (19 month old)


title: m2_Aged_L10_new_384

description: Aged (19 month old)


title: m2_Aged_D6_new_384

description: Aged (19 month old)


title: m2_Aged_M8_new_384

description: Aged (19 month old)


title: m2_Aged_D17_new_384

description: Aged (19 month old)


title: m2_Young_E12_new_384

description: Young (3 month old)


title: m2_Aged_P22_new_384

description: Aged (19 month old)


title: m2_Young_O22_new_384

description: Young (3 month old)


title: m2_Aged_M9_new_384

description: Aged (19 month old)


title: m2_Aged_O18_new_384

description: Aged (19 month old)


title: m2_Aged_M6_new_384

description: Aged (19 month old)


title: m2_Young_B18_new_384

description: Young (3 month old)


title: m2_Young_K5_new_384

description: Young (3 month old)


title: m2_Young_K4_new_384

description: Young (3 month old)


title: m2_Young_G18_new_384

description: Young (3 month old)


title: m2_Aged_H19_new_384

description: Aged (19 month old)


title: m2_Aged_F6_new_384

description: Aged (19 month old)


title: m2_Young_K3_new_384

description: Young (3 month old)


title: m2_Aged_E16_new_384

description: Aged (19 month old)


title: m2_Young_H7_new_384

description: Young (3 month old)


title: m2_Young_N15_new_384

description: Young (3 month old)


title: m2_Aged_E23_new_384

description: Aged (19 month old)


title: m2_Aged_I9_new_384

description: Aged (19 month old)


title: m2_Aged_D10_new_384

description: Aged (19 month old)


title: m2_Young_A20_new_384

description: Young (3 month old)


title: m1_Young_N19_new_384

description: Young (3 month old)


title: m2_Young_M20_new_384

description: Young (3 month old)


title: m2_Young_D16_new_384

description: Young (3 month old)


title: m2_Young_J18_new_384

description: Young (3 month old)


title: m1_Young_E6_new_384

description: Young (3 month old)


title: m1_Young_I16_new_384

description: Young (3 month old)


title: m1_Young_O20_new_384

description: Young (3 month old)


title: m1_Young_D8_new_384

description: Young (3 month old)


title: m1_Aged_I17_new_384

description: Aged (19 month old)


title: m1_Aged_E24_new_384

description: Aged (19 month old)


title: m1_Young_C10_new_384

description: Young (3 month old)


title: m1_Young_F22_new_384

description: Young (3 month old)


title: m1_Young_J6_new_384

description: Young (3 month old)


title: m1_Aged_B19_new_384

description: Aged (19 month old)


title: m1_Young_O14_new_384

description: Young (3 month old)


title: m1_Young_A20_new_384

description: Young (3 month old)


title: m1_Aged_P3_new_384

description: Aged (19 month old)


title: m1_Aged_F6_new_384

description: Aged (19 month old)


title: m1_Young_H22_new_384

description: Young (3 month old)


title: m1_Young_I6_new_384

description: Young (3 month old)


title: m1_Aged_O16_new_384

description: Aged (19 month old)


title: m1_Young_N16_new_384

description: Young (3 month old)


title: m1_Aged_I14_new_384

description: Aged (19 month old)


title: m1_Young_K15_new_384

description: Young (3 month old)


title: m2_Aged_E14_new_384

description: Aged (19 month old)


title: m1_Aged_P24_new_384

description: Aged (19 month old)


title: m1_Young_C17_new_384

description: Young (3 month old)


title: m1_Young_M11_new_384

description: Young (3 month old)


title: m1_Young_N13_new_384

description: Young (3 month old)


title: m1_Young_A17_new_384

description: Young (3 month old)


title: m1_Aged_K18_new_384

description: Aged (19 month old)


title: m1_Young_D14_new_384

description: Young (3 month old)


title: m1_Aged_H8_new_384

description: Aged (19 month old)


title: m1_Young_K21_new_384

description: Young (3 month old)


title: m1_Young_C14_new_384

description: Young (3 month old)


title: m1_Aged_D15_new_384

description: Aged (19 month old)


title: m1_Young_A4_new_384

description: Young (3 month old)


title: m1_Young_J16_new_384

description: Young (3 month old)


title: m1_Young_C11_new_384

description: Young (3 month old)


title: m1_Aged_C9_new_384

description: Aged (19 month old)


title: m1_Young_H15_new_384

description: Young (3 month old)


title: m1_Young_N15_new_384

description: Young (3 month old)


title: m1_Young_B12_new_384

description: Young (3 month old)


title: m1_Young_M17_new_384

description: Young (3 month old)


title: m1_Aged_G4_new_384

description: Aged (19 month old)


title: m1_Young_L11_new_384

description: Young (3 month old)


title: m1_Young_L22_new_384

description: Young (3 month old)


title: m1_Aged_H15_new_384

description: Aged (19 month old)


title: m1_Young_J10_new_384

description: Young (3 month old)


title: m1_Young_J20_new_384

description: Young (3 month old)


title: m1_Young_N14_new_384

description: Young (3 month old)


title: m1_Young_F10_new_384

description: Young (3 month old)


title: m1_Young_A16_new_384

description: Young (3 month old)


title: m1_Young_I18_new_384

description: Young (3 month old)


title: m1_Young_C8_new_384

description: Young (3 month old)


title: m1_Young_P7_new_384

description: Young (3 month old)


title: m1_Young_M21_new_384

description: Young (3 month old)


title: m1_Young_B8_new_384

description: Young (3 month old)


title: m1_Young_I20_new_384

description: Young (3 month old)


title: m1_Aged_C10_new_384

description: Aged (19 month old)


title: m1_Young_O19_new_384

description: Young (3 month old)


title: m1_Young_G14_new_384

description: Young (3 month old)


title: m1_Young_B6_new_384

description: Young (3 month old)


title: m1_Young_M22_new_384

description: Young (3 month old)


title: m1_Aged_L21_new_384

description: Aged (19 month old)


title: m1_Young_I10_new_384

description: Young (3 month old)


title: m1_Young_F19_new_384

description: Young (3 month old)


title: m1_Young_K14_new_384

description: Young (3 month old)


title: m1_Young_A8_new_384

description: Young (3 month old)


title: m1_Young_O17_new_384

description: Young (3 month old)


title: m1_Young_O7_new_384

description: Young (3 month old)


title: m1_Young_E17_new_384

description: Young (3 month old)


title: m1_Aged_G18_new_384

description: Aged (19 month old)


title: m1_Young_H13_new_384

description: Young (3 month old)


title: m1_Young_K11_new_384

description: Young (3 month old)


title: m1_Aged_G12_new_384

description: Aged (19 month old)


title: m1_Young_H12_new_384

description: Young (3 month old)


title: m1_Young_F14_new_384

description: Young (3 month old)


title: m1_Young_N12_new_384

description: Young (3 month old)


title: m1_Young_K22_new_384

description: Young (3 month old)


title: m1_Young_C15_new_384

description: Young (3 month old)


title: m1_Young_J19_new_384

description: Young (3 month old)


title: m1_Aged_K7_new_384

description: Aged (19 month old)


title: m1_Young_B5_new_384

description: Young (3 month old)


title: m1_Young_D20_new_384

description: Young (3 month old)


title: m1_Young_D16_new_384

description: Young (3 month old)


title: m1_Young_M12_new_384

description: Young (3 month old)


title: m1_Aged_F4_new_384

description: Aged (19 month old)


title: m1_Young_F11_new_384

description: Young (3 month old)


title: m1_Young_G19_new_384

description: Young (3 month old)


title: m1_Young_O10_new_384

description: Young (3 month old)


title: m1_Young_C22_new_384

description: Young (3 month old)


title: m1_Young_D13_new_384

description: Young (3 month old)


title: m1_Aged_H19_new_384

description: Aged (19 month old)


title: m1_Young_L21_new_384

description: Young (3 month old)


title: m1_Aged_B12_new_384

description: Aged (19 month old)


title: m1_Young_C16_new_384

description: Young (3 month old)


title: m1_Young_A13_new_384

description: Young (3 month old)


title: m1_Young_J22_new_384

description: Young (3 month old)


title: m1_Young_D18_new_384

description: Young (3 month old)


title: m1_Young_D15_new_384

description: Young (3 month old)


title: m1_Aged_F20_new_384

description: Aged (19 month old)


title: m1_Young_K17_new_384

description: Young (3 month old)


title: m1_Young_G20_new_384

description: Young (3 month old)


title: m1_Young_C21_new_384

description: Young (3 month old)


title: m1_Young_B17_new_384

description: Young (3 month old)


title: m1_Young_B9_new_384

description: Young (3 month old)


title: m1_Young_O15_new_384

description: Young (3 month old)


title: m1_Young_A9_new_384

description: Young (3 month old)


title: m1_Young_N21_new_384

description: Young (3 month old)


title: m1_Young_M6_new_384

description: Young (3 month old)


title: m1_Young_D11_new_384

description: Young (3 month old)


title: m1_Young_M20_new_384

description: Young (3 month old)


title: m1_Young_C19_new_384

description: Young (3 month old)


title: m1_Young_L19_new_384

description: Young (3 month old)


title: m1_Young_B11_new_384

description: Young (3 month old)


title: m1_Young_O16_new_384

description: Young (3 month old)


title: m1_Young_J21_new_384

description: Young (3 month old)


title: m1_Young_B15_new_384

description: Young (3 month old)


title: m1_Young_I21_new_384

description: Young (3 month old)


title: m1_Young_M5_new_384

description: Young (3 month old)


title: m1_Young_N22_new_384

description: Young (3 month old)


title: m1_Young_G10_new_384

description: Young (3 month old)


title: m1_Aged_M5_new_384

description: Aged (19 month old)


title: m1_Aged_J15_new_384

description: Aged (19 month old)


title: m1_Young_K9_new_384

description: Young (3 month old)


title: m1_Young_C9_new_384

description: Young (3 month old)


title: m1_Young_J12_new_384

description: Young (3 month old)


title: m1_Aged_D9_new_384

description: Aged (19 month old)


title: m1_Young_G16_new_384

description: Young (3 month old)


title: m1_Young_L15_new_384

description: Young (3 month old)


title: m1_Aged_N12_new_384

description: Aged (19 month old)


title: m1_Young_A14_new_384

description: Young (3 month old)


title: m1_Young_N7_new_384

description: Young (3 month old)


title: m1_Young_B14_new_384

description: Young (3 month old)


title: m1_Young_G18_new_384

description: Young (3 month old)


title: m1_Aged_O4_new_384

description: Aged (19 month old)


title: m1_Aged_L20_new_384

description: Aged (19 month old)


title: m1_Aged_D6_new_384

description: Aged (19 month old)


title: m1_Aged_C4_new_384

description: Aged (19 month old)


title: m1_Young_G9_new_384

description: Young (3 month old)


title: m1_Aged_B21_new_384

description: Aged (19 month old)


title: m1_Young_H19_new_384

description: Young (3 month old)


title: m1_Aged_I18_new_384

description: Aged (19 month old)


title: m1_Young_D17_new_384

description: Young (3 month old)


title: m1_Aged_E21_new_384

description: Aged (19 month old)


title: m1_Young_L4_new_384

description: Young (3 month old)


title: m1_Young_K6_new_384

description: Young (3 month old)


title: m1_Young_I8_new_384

description: Young (3 month old)


title: m1_Aged_L9_new_384

description: Aged (19 month old)


title: m1_Young_C20_new_384

description: Young (3 month old)


title: m1_Aged_M15_new_384

description: Aged (19 month old)


title: m1_Aged_O19_new_384

description: Aged (19 month old)


title: m1_Aged_K20_new_384

description: Aged (19 month old)


title: m1_Young_J9_new_384

description: Young (3 month old)


title: m1_Aged_J10_new_384

description: Aged (19 month old)


title: m1_Aged_C21_new_384

description: Aged (19 month old)


title: m1_Aged_I24_new_384

description: Aged (19 month old)


title: m1_Young_C13_new_384

description: Young (3 month old)


title: m1_Young_P21_new_384

description: Young (3 month old)


title: 129-Mbnl1KO_Thymus_A01243

city: Las Vegas

first: Łukasz

middle: J.

last: Sznajder

state: Nevada

address: 4505 S. Maryland Pkwy.

department: Chemistry and Biochemistry

institute: University of Nevada-Las Vegas (UNLV)

description: rRNA depleted total RNA

data_processing:
Basecalling was done using Illumina Real-Time Analysis (RTA) and demultiplexed fastq files were obtained from BaseSpace with BaseMount.
STAR was used to map reads to the reference genome.
Salmon and DEseq2 were used to quantify and normalize gene expression followed by differential analysis.
rMATS turbo was used for differential alternative splicing.
IRFinder was used for retained intron quantification.
Genome_build: mm10 and hg38.
Supplementary_files_format_and_content: Sznajder_Supplementary_Data.xlsx contains STAR statistics as well as DESeq2, rMATS and IRFinder results.

source_name: Thymus

extract_protocol:
Total RNA from DNA/RNA Shield Blood Collection Tubes was isolated by using Quick-DNA/RNA Blood Tube Kit with DNase treatment according to manufacturer’s provided protocol (Zymo Research). Total RNA from PBMCs was isolated by using TRIzol Reagent and the Direct-zol RNA MiniPrep Kit with DNase treatment according to manufacturer’s provided protocol (Zymo Research). Total RNA from mouse thymus was isolated using PolyTron (Kinematica) homogenization in TRI Reagent (Sigma) followed by treatment with the RNeasy Kit (Qiagen) according to the manufacturer’s instructions. RNA was quantitated on a Nanodrop, Quant-iT RiboGreen RNA Assay Kit (Thermo Fisher) and/or Qubit RNA BR Assay Kit (Thermo Fisher Scientific).
Thymic RNA-seq (2 × 50 paired-end reads) were performed by The New York Genome Center from total RNA. For human PBMC and whole blood strand-specific, rRNA- and globin- (only whole blood) depleted RNA-seq libraries were prepared using the KAPA Stranded RNA-seq Kit with RiboErase HMR Globin (Kapa Biosystems) per manufacturer’s instructions, except for the use of custom Illumina-compatible index primers to allow multiplexing. Library size distribution was assessed using the High Sensitivity NGS Fragment Analysis Kit (DNF-747) on a Fragment Analyzer (Agilent) and 2 × 76 paired-end sequencing was performed using an Illumina NextSeq 500.

tag: condition

value: Mbnl1 knockout

tag: strain

tag: Sex

value: Male

tag: sampling age

value: Postnatal day 63


title: 129-Mbnl1KO_Thymus_A01242

description: rRNA depleted total RNA


title: m1_Young_G4_new_384

description: Young (3 month old)


title: 129-Mbnl1KO_Thymus_A01241

description: rRNA depleted total RNA


title: m1_Young_L9_new_384

description: Young (3 month old)


title: RNAseq_KPG_rep3

city: West Haven

first: Francesc

last: Lopez

state: CT

department: Department of Genetics

institute: Yale University

data_processing:
Basecalls performed using CASAVA version 1.8.2
For each read, the first 6 and the last nucleotides were trimmed to the point where the Phred score of an examined base fell below 20 using in-house scripts. If, after trimming, the read was shorter than 45 bp, the whole read was discarded.
Mapping with HISAT2 v2.1
Gene counts were produced with StringTie v1.3.3b and the Python script “prepDE.py” provided in the package
DESeq2 was used for differential expression analysis
Genome_build: mm10
Supplementary_files_format_and_content: txt (Gene Counts per each sample, and Deseq2 results)
Supplementary_files_format_and_content: normalized counts

source_name: Murine cell line

growth_protocol: Cells were grown in monolayer in RPMI + 10% FBS + Penicillin-streptomycin and amphotericin at 37ºC, 5% CO2 for 3 days

extract_protocol:
Total RNA was extracted using Rneasy Qiagen Kit with on-column Dnase treatment.
RNA libraries were prepared for sequencing using standard Illumina protocols

tag: tissue

value: Lung

tag: cell line

value: KPG

tag: genotype

value: KrasG12D p53-/- Gata6-/-


title: m1_Aged_I20_new_384

description: Aged (19 month old)


title: RNAseq_KPG_rep1


title: m1_Young_D22_new_384

description: Young (3 month old)


title: RNAseq_KPG_rep2


title: RNAseq_KP_rep2

value: SPC-Cre KP

value: KrasG12D p53-/-


title: RNAseq_KP_rep3


title: RNAseq_KP_rep1


title: 129-WT_Thymus_AJAX14

description: rRNA depleted total RNA

value: Wild type


title: 129-WT_Thymus_AJAX15

description: rRNA depleted total RNA


title: 129-WT_Thymus_AJAX16

description: rRNA depleted total RNA


title: m1_Young_E7_new_384

description: Young (3 month old)


title: m1_Young_M7_new_384

description: Young (3 month old)


title: m1_Aged_J9_new_384

description: Aged (19 month old)


title: m1_Young_F3_new_384

description: Young (3 month old)


title: m1_Aged_C24_new_384

description: Aged (19 month old)


title: m1_Aged_G14_new_384

description: Aged (19 month old)


title: m1_Young_M15_new_384

description: Young (3 month old)


title: XY_IVD_ag9-1_S5_L001

city: Fukuoka

first: Nobuhiko

last: Hamazaki

state: Fukuoka

address: Maidashi 3-1-1, Higashi-ku,

department: Department of Developmental Stem Cell Biology

country: Japan

institute: Kyushu University

data_processing:
RNA-seq reads were processed with the FASTX tool kit(Schmieder and Edwards, 2011) to remove short (<20 bp) and low quality (quality score <20) reads, followed by trimming of the adaptor sequence.
Processed reads were mapped to the mouse mm10 genome using STAR(Dobin et al., 2013).
For the counting of uniquely and multiply mapped reads on RefSeq mRNAs, featureCount(Liao, Smyth and Shi, 2014). was used with parameter -O -s 0 or -M -O -s 0, respectively.
Since we noticed that some of the libraries showed abnormally high levels of Interleukin 2 (Il2) transcript presumably because of contamination, we therefore excluded Il2 for further analysis.
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include TPM values for each Sample.

molecule: polyA RNA

growth_protocol: The ESCs used in all experiments were maintained under a 2i+LIF condition. EpiLCs, PGCLCs and oocytes were differentiated from ESCs as described previously (Hayashi et al., 2013, Hikabe et al., 2016), purified by FACSAriaTM II (BD Bioscience).

extract_protocol:
Non-directional and directional RNA-seq library was constructed, as described previously (Hikabe et al., 2016). Briefly, poly(A)+ RNAs were purified from 200-40,000 cells or oocytes (ESCs, EpiLCs and d6PGCLCs: 10,000; IVDi ag3-13: 200-40,000), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). SC-positive oocytes were collected using a FACS Aria II (BD Bioscience). Biologically duplicated (ESCs, EpiLCs, agg-9, agg-11 and agg-13) or triplicated (PGCLCs, agg-3, agg-5 and agg-7) were prepared.
Purified RNAs were subjected to library construction using a NEBNext Ultra non-Directional RNA Library Prep Kit for Illumina (NEB) for ESCs, EpiLCs, PGCLCs and agg3-7 and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) for agg9-13. cDNAs were enriched by 12-cycle PCR. Sequencing of the libraries was performed with a HiSeq 2500 (Illumina).

tag: genotype/variation

value: XY

value: Re-aggregated XY PGCLCs with female E12.5 gonadal somatic cells for 9 days


title: m1_Aged_G11_new_384

description: Aged (19 month old)


title: XY_IVD_ag9-2_S6_L001


title: m1_Young_L14_new_384

description: Young (3 month old)


title: XY_IVD_ag13-2_S18_L002

value: Re-aggregated XY PGCLCs with female E12.5 gonadal somatic cells for 13 days


title: XY_IVD_ag13-1_S17_L002


title: m1_Aged_M20_new_384

description: Aged (19 month old)


title: m1_Young_B21_new_384

description: Young (3 month old)


title: m1_Young_A21_new_384

description: Young (3 month old)


title: XY_IVD_ag11-2_S12_L001

value: Re-aggregated XY PGCLCs with female E12.5 gonadal somatic cells for 11 days


title: m1_Aged_F21_new_384

description: Aged (19 month old)


title: m1_Aged_D19_new_384

description: Aged (19 month old)


title: XX_IVD_ag9-2_S26_L002

value: XX

value: Re-aggregated XX PGCLCs with female E12.5 gonadal somatic cells for 9 days


title: m1_Aged_O11_new_384

description: Aged (19 month old)


title: m1_Young_C6_new_384

description: Young (3 month old)


title: XX_IVD_ag9-1_S1_L001


title: XX_IVD_ag13-1_S13_L001

value: Re-aggregated XX PGCLCs with female E12.5 gonadal somatic cells for 13 days


title: XX_IVD_ag11-2_S8_L001

value: Re-aggregated XX PGCLCs with female E12.5 gonadal somatic cells for 11 days


title: m1_Young_E18_new_384

description: Young (3 month old)

value: primary murine brain endothelial cells


title: XX_IVD_ag11-1_S7_L001


title: XO_IVD_ag9-2_S4_L001

value: XO

value: Re-aggregated XO PGCLCs with female E12.5 gonadal somatic cells for 9 days


title: XY_IVD_ag11-1_S11_L001


title: m1_Young_M9_new_384

description: Young (3 month old)


title: m1_Young_K12_new_384

description: Young (3 month old)


title: XO_IVD_ag13-2_S16_L002

value: Re-aggregated XO PGCLCs with female E12.5 gonadal somatic cells for 13 days


title: XO_IVD_ag9-1_S3_L001


title: m1_Young_P9_new_384

description: Young (3 month old)


title: XO_IVD_ag11-2_S10_L001

value: Re-aggregated XO PGCLCs with female E12.5 gonadal somatic cells for 11 days


title: m1_Young_M18_new_384

description: Young (3 month old)


title: XX_IVD_ag13-2_S14_L002


title: XY_d6PGCLC-3_S18_L002

value: PGCLCs induced from XY EpiLCs for 6 days


title: m1_Young_H21_new_384

description: Young (3 month old)


title: m1_Aged_L5_new_384

description: Aged (19 month old)


title: XO_IVD_ag13-1_S15_L002


title: m1_Young_K5_new_384

description: Young (3 month old)


title: XO_IVD_ag11-1_S9_L001


title: XY_d6PGCLC-1_S16_L002


title: m1_Young_J17_new_384

description: Young (3 month old)


title: m1_Young_G15_new_384

description: Young (3 month old)


title: m1_Young_A12_new_384

description: Young (3 month old)


title: XY_IVD_ag7-2_S44_L004

value: Re-aggregated XY PGCLCs with female E12.5 gonadal somatic cells for 7 days


title: XY_d2EpiLC-1_S9_L001

value: EpiLCs induced from XY ESCs for 2days


title: XY_IVD_ag7-1_S43_L004


title: XY_IVD_ag7-3_S45_L004


title: XY_d6PGCLC-2_S17_L002


title: XY_IVD_ag5-3_S36_L003

value: Re-aggregated XY PGCLCs with female E12.5 gonadal somatic cells for 5 days


title: m1_Aged_K12_new_384

description: Aged (19 month old)


title: m1_Aged_B3_new_384

description: Aged (19 month old)


title: XY_d2EpiLC-2_S10_L001

value: XY


title: m1_Aged_J16_new_384

description: Aged (19 month old)


title: m1_Aged_D21_new_384

description: Aged (19 month old)


title: XY_IVD_ag3-3_S27_L003

value: Re-aggregated XY PGCLCs with female E12.5 gonadal somatic cells for 3 days


title: XY_IVD_ag5-2_S35_L003


title: XY_IVD_ag5-1_S34_L003


title: m1_Young_H20_new_384

description: Young (3 month old)


title: m1_Aged_G10_new_384

description: Aged (19 month old)


title: m1_Young_L5_new_384

description: Young (3 month old)


title: m1_Aged_K14_new_384

description: Aged (19 month old)


title: XY_IVD_ag3-2_S26_L003


title: m1_Aged_J13_new_384

description: Aged (19 month old)


title: m1_Aged_P21_new_384

description: Aged (19 month old)


title: m1_Aged_K8_new_384

description: Aged (19 month old)


title: m1_Young_I22_new_384

description: Young (3 month old)


title: XX_d6PGCLC-3_S15_L002

value: PGCLCs induced from XX EpiLCs for 6 days


title: XX_d6PGCLC-1_S13_L002


title: XY_ESC-1_S3_L001

value: Mouse XY ESCs cultured in 2i+LIF medium.


title: XX_d2EpliLC-2_S8_L001

value: XX

value: EpiLCs induced from XX ESCs for 2days


title: m1_Aged_N20_new_384

description: Aged (19 month old)


title: XX_d2EpliLC-1_S7_L001


title: XX_d6PGCLC-2_S14_L002


title: m1_Aged_B7_new_384

description: Aged (19 month old)


title: XX_IVD_ag7-2_S41_L004

value: Re-aggregated XX PGCLCs with female E12.5 gonadal somatic cells for 7 days


title: m1_Aged_M19_new_384

description: Aged (19 month old)


title: XY_ESC-2_S4_L001


title: m1_Aged_E20_new_384

description: Aged (19 month old)


title: XY_IVD_ag3-1_S25_L003


title: m1_Young_F4_new_384

description: Young (3 month old)


title: m1_Aged_F18_new_384

description: Aged (19 month old)


title: XX_IVD_ag5-3_S33_L003

value: Re-aggregated XX PGCLCs with female E12.5 gonadal somatic cells for 5 days


title: XX_IVD_ag7-1_S40_L004


title: XX_IVD_ag5-1_S31_L003


title: m1_Aged_L7_new_384

description: Aged (19 month old)

value: primary murine brain endothelial cells


title: XX_IVD_ag5-2_S32_L003


title: m1_Aged_G24_new_384

description: Aged (19 month old)


title: m1_Aged_F12_new_384

description: Aged (19 month old)


title: m1_Young_P5_new_384

description: Young (3 month old)


title: XX_IVD_ag3-2_S23_L002

value: Re-aggregated XX PGCLCs with female E12.5 gonadal somatic cells for 3 days


title: XX_IVD_ag3-3_S24_L002


title: XX_IVD_ag3-1_S22_L002


title: m1_Young_B18_new_384

description: Young (3 month old)


title: XX_ESC-2_S2_L001

value: Mouse XX ESCs cultured in 2i+LIF medium.


title: m1_Aged_C5_new_384

description: Aged (19 month old)


title: XX_ESC-1_S1_L001


title: m1_Young_P6_new_384

description: Young (3 month old)


title: XO_d6PGCLC-2_S20_L002

value: XO

value: PGCLCs induced from XO EpiLCs for 6 days


title: m1_Aged_K9_new_384

description: Aged (19 month old)


title: m1_Young_M14_new_384

description: Young (3 month old)


title: m1_Aged_K5_new_384

description: Aged (19 month old)


title: m1_Aged_J4_new_384

description: Aged (19 month old)


title: XO_d6PGCLC-1_S19_L002


title: m1_Young_O3_new_384

description: Young (3 month old)


title: XO_d6PGCLC-3_S21_L002


title: m1_Aged_A4_new_384

description: Aged (19 month old)


title: XO_IVD_ag7-2_S47_L004

value: Re-aggregated XO PGCLCs with female E12.5 gonadal somatic cells for 7 days


title: XO_d2EpiLC-1_S11_L001

value: EpiLCs induced from XO ESCs for 2days


title: XO_IVD_ag7-1_S46_L004


title: m1_Aged_M14_new_384

description: Aged (19 month old)


title: m1_Aged_B6_new_384

description: Aged (19 month old)


title: XO_d2EpiLC-2_S12_L001


title: m1_Young_B10_new_384

description: Young (3 month old)


title: XO_IVD_ag5-3_S39_L004

value: Re-aggregated XO PGCLCs with female E12.5 gonadal somatic cells for 5 days


title: m1_Aged_I19_new_384

description: Aged (19 month old)


title: XO_IVD_ag5-2_S38_L004


title: m1_Young_P18_new_384

description: Young (3 month old)


title: m1_Young_F21_new_384

description: Young (3 month old)


title: m1_Young_F18_new_384

description: Young (3 month old)


title: XO_IVD_ag3-3_S30_L003

value: Re-aggregated XO PGCLCs with female E12.5 gonadal somatic cells for 3 days


title: XO_IVD_ag5-1_S37_L004


title: XO_IVD_ag3-2_S29_L003


title: m1_Aged_F24_new_384

description: Aged (19 month old)


title: XO_IVD_ag3-1_S28_L003


title: m1_Young_P22_new_384

description: Young (3 month old)


title: m1_Aged_P19_new_384

description: Aged (19 month old)


title: m1_Aged_P22_new_384

description: Aged (19 month old)


title: m1_Aged_E16_new_384

description: Aged (19 month old)


title: XO_IVD_ag7-3_S48_L004


title: m1_Young_L16_new_384

description: Young (3 month old)


title: XO_ESC-1_S5_L001

value: Mouse XO ESCs cultured in 2i+LIF medium.


title: m1_Aged_E19_new_384

description: Aged (19 month old)


title: m1_Young_P16_new_384

description: Young (3 month old)


title: m1_Young_K18_new_384

description: Young (3 month old)


title: m1_Aged_C12_new_384

description: Aged (19 month old)


title: m1_Young_A10_new_384

description: Young (3 month old)


title: m1_Aged_E3_new_384

description: Aged (19 month old)


title: m1_Aged_I8_new_384

description: Aged (19 month old)


title: m1_Aged_G8_new_384

description: Aged (19 month old)


title: m1_Aged_O21_new_384

description: Aged (19 month old)


title: m1_Aged_L17_new_384

description: Aged (19 month old)


title: m1_Young_F15_new_384

description: Young (3 month old)


title: m1_Aged_G6_new_384

description: Aged (19 month old)


title: m1_Young_D19_new_384

description: Young (3 month old)


title: m1_Aged_B5_new_384

description: Aged (19 month old)


title: m1_Aged_D14_new_384

description: Aged (19 month old)


title: m1_Young_I4_new_384

description: Young (3 month old)


title: m1_Young_G11_new_384

description: Young (3 month old)


title: XO_ESC-2_S6_L001


title: m1_Aged_F15_new_384

description: Aged (19 month old)


title: m1_Young_L10_new_384

description: Young (3 month old)


title: m1_Young_N10_new_384

description: Young (3 month old)


title: m1_Young_E10_new_384

description: Young (3 month old)


title: m1_Aged_H17_new_384

description: Aged (19 month old)


title: m1_Young_M8_new_384

description: Young (3 month old)


title: m1_Young_K20_new_384

description: Young (3 month old)


title: m1_Young_E8_new_384

description: Young (3 month old)


title: m1_Young_N11_new_384

description: Young (3 month old)


title: m1_Young_D5_new_384

description: Young (3 month old)


title: m1_Aged_G21_new_384

description: Aged (19 month old)


title: m1_Young_J4_new_384

description: Young (3 month old)


title: m1_Young_B20_new_384

description: Young (3 month old)


title: m1_Young_F17_new_384

description: Young (3 month old)


title: m1_Aged_G5_new_384

description: Aged (19 month old)


title: m1_Aged_H21_new_384

description: Aged (19 month old)


title: m1_Young_F20_new_384

description: Young (3 month old)


title: m1_Aged_D16_new_384

description: Aged (19 month old)


title: m1_Young_L12_new_384

description: Young (3 month old)


title: m1_Young_L8_new_384

description: Young (3 month old)


title: m1_Aged_H20_new_384

description: Aged (19 month old)


title: m1_Aged_M16_new_384

description: Aged (19 month old)


title: m1_Young_H9_new_384

description: Young (3 month old)


title: m1_Young_K10_new_384

description: Young (3 month old)


title: m1_Young_H14_new_384

description: Young (3 month old)


title: m1_Aged_J18_new_384

description: Aged (19 month old)


title: m1_Young_A23_new_384

description: Young (3 month old)


title: m1_Aged_G7_new_384

description: Aged (19 month old)


title: m1_Young_L18_new_384

description: Young (3 month old)


title: m1_Young_G8_new_384

description: Young (3 month old)


title: m1_Young_B19_new_384

description: Young (3 month old)


title: m1_Aged_M13_new_384

description: Aged (19 month old)


title: m1_Young_I13_new_384

description: Young (3 month old)


title: m1_Aged_K16_new_384

description: Aged (19 month old)


title: m1_Aged_F14_new_384

description: Aged (19 month old)


title: m1_Young_H11_new_384

description: Young (3 month old)


title: m1_Young_M19_new_384

description: Young (3 month old)


title: m1_Aged_D17_new_384

description: Aged (19 month old)


title: m1_Young_O8_new_384

description: Young (3 month old)


title: m1_Young_C18_new_384

description: Young (3 month old)


title: m1_Young_I12_new_384

description: Young (3 month old)


title: m1_Aged_G19_new_384

description: Aged (19 month old)


title: m1_Aged_C18_new_384

description: Aged (19 month old)


title: m1_Aged_L18_new_384

description: Aged (19 month old)


title: m1_Aged_O3_new_384

description: Aged (19 month old)


title: m1_Young_O11_new_384

description: Young (3 month old)


title: m1_Aged_G15_new_384

description: Aged (19 month old)


title: m1_Aged_L3_new_384

description: Aged (19 month old)


title: m1_Young_E9_new_384

description: Young (3 month old)


title: m1_Young_A22_new_384

description: Young (3 month old)


title: Atopic wheezer rep13 nasal UNPAIRED

city: Crawley, Perth

first: Emma

last: de Jong

state: WA

address: 35 Stirling Hwy

country: Australia

institute: University of Western Australia

description: Sample name: AF098

data_processing:
Image analysis was performed in real time by the HiSeq Control Software (HCS) v2.2.68 and Real Time Analysis (RTA) v1.18.66.3, running on the instrument computer.
the Illumina bcl2fastq 2.17.1.14 pipeline was used to generate the sequence data.
Read libraries were mapped to the GRCh37 (hg19) Human genome using HISAT2 (v2.0.4)
Gene-level quantitation (counts) of HISAT2 alignments was performed using SummarizeOverlaps
Genome_build: hg19
Supplementary_files_format_and_content: genecounts.csv file contains a matrix of raw gene counts (unfiltered and un-normalised data)

organism: Homo sapiens

source_name: Nasal epithelium

extract_protocol:
RNA was extracted using the Ambion Purelink® RNA mini kit (Thermo Scientific) per the manufacturer’s instructions.
RNA libraries were prepared for sequencing using standard Illumina protocols

treatment_protocol: Approximately one million AECs were fractionated out from the initially collected samples, pelleted and resuspended in 350 µL RLT buffer containing 1% (v/v) ß-mercaptoethanol (QIAGEN) and stored at -80°C until all participants were successfully recruited and samples obtained.

tag: diagnosis

value: Atopic asthmatic

value: Nasal epithelium

tag: gender

value: F

tag: age (yrs)


title: m1_Aged_I12_new_384

description: Aged (19 month old)


title: Atopic wheezer rep14 nasal UNPAIRED

description: Sample name: AF103

value: M


title: m1_Young_N9_new_384

description: Young (3 month old)


title: m1_Young_L3_new_384

description: Young (3 month old)


title: m1_Aged_C20_new_384

description: Aged (19 month old)


title: Atopic wheezer rep12 nasal UNPAIRED

description: Sample name: AF092


title: m1_Aged_F3_new_384

description: Aged (19 month old)


title: Atopic wheezer rep11 nasal UNPAIRED

description: Sample name: AF085


title: m1_Young_O13_new_384

description: Young (3 month old)


title: Atopic wheezer rep10 nasal

description: Sample name: AF078


title: m1_Aged_C19_new_384

description: Aged (19 month old)


title: Atopic wheezer rep10 tracheal

description: Sample name: AF079

source_name: Bronchial epithelium

value: Bronchial epithelium


title: m1_Aged_K21_new_384

description: Aged (19 month old)


title: m1_Young_A19_new_384

description: Young (3 month old)


title: m1_Young_F9_new_384

description: Young (3 month old)


title: m1_Young_D21_new_384

description: Young (3 month old)


title: m1_Young_E16_new_384

description: Young (3 month old)


title: Atopic wheezer rep11 tracheal UNPAIRED

description: Sample name: AF086


title: m1_Aged_B20_new_384

description: Aged (19 month old)


title: m1_Aged_C11_new_384

description: Aged (19 month old)


title: m3_Young_J18_new_384

description: Young (3 month old)


title: Atopic wheezer rep9 nasal

description: Sample name: AF071


title: Atopic wheezer rep8 tracheal

description: Sample name: AF064


title: Atopic wheezer rep9 tracheal

description: Sample name: AF072


title: m3_Aged_O23_new_384

description: Aged (19 month old)


title: Atopic wheezer rep7 tracheal

description: Sample name: AF056


title: Atopic wheezer rep7 nasal

description: Sample name: AF055


title: Atopic wheezer rep8 nasal

description: Sample name: AF063


title: m3_Young_M5_new_384

description: Young (3 month old)


title: m3_Young_N23_new_384

description: Young (3 month old)


title: m3_Aged_K21_new_384

description: Aged (19 month old)


title: m3_Young_G7_new_384

description: Young (3 month old)


title: Atopic wheezer rep6 nasal

description: Sample name: AF047


title: Atopic wheezer rep4 tracheal

description: Sample name: AF032


title: Atopic wheezer rep4 nasal

description: Sample name: AF031


title: m3_Aged_O5_new_384

description: Aged (19 month old)


title: Atopic wheezer rep3 tracheal

description: Sample name: AF024


title: Atopic wheezer rep5 tracheal

description: Sample name: AF040


title: m3_Aged_I4_new_384

description: Aged (19 month old)


title: Atopic wheezer rep6 tracheal

description: Sample name: AF048


title: Atopic wheezer rep5 nasal

description: Sample name: AF039


title: Atopic wheezer rep3 nasal

description: Sample name: AF023


title: m3_Aged_G5_new_384

description: Aged (19 month old)


title: m3_Young_G15_new_384

description: Young (3 month old)


title: m3_Young_O4_new_384

description: Young (3 month old)


title: m3_Aged_O22_new_384

description: Aged (19 month old)


title: XX_IVD_ag7-3_S42_L004

value: XX

value: Re-aggregated XX PGCLCs with female E12.5 gonadal somatic cells for 7 days


title: m3_Aged_D19_new_384

description: Aged (19 month old)

value: primary murine brain endothelial cells


title: Atopic non-wheezer rep15 tracheal UNPAIRED

description: Sample name: AF107

value: Atopic non-asthmatic


title: m3_Aged_A17_new_384

description: Aged (19 month old)


title: Atopic wheezer rep2 tracheal

description: Sample name: AF016


title: m3_Aged_O3_new_384

description: Aged (19 month old)


title: m3_Aged_A19_new_384

description: Aged (19 month old)


title: m3_Young_I5_new_384

description: Young (3 month old)


title: Atopic non-wheezer rep12 tracheal

description: Sample name: AF090


title: Atopic non-wheezer rep12 nasal

description: Sample name: AF089


title: m3_Aged_P13_new_384

description: Aged (19 month old)


title: m3_Young_K15_new_384

description: Young (3 month old)


title: m3_Aged_M2_new_384

description: Aged (19 month old)


title: Atopic wheezer rep2 nasal

description: Sample name: AF015


title: Atopic non-wheezer rep11 tracheal

description: Sample name: AF083


title: Atopic wheezer rep1 tracheal

description: Sample name: AF008


title: m3_Aged_C15_new_384

description: Aged (19 month old)


title: Atopic wheezer rep1 nasal

description: Sample name: AF007


title: Atopic non-wheezer rep10 tracheal

description: Sample name: AF076


title: m3_Aged_H9_new_384

description: Aged (19 month old)


title: m3_Young_H22_new_384

description: Young (3 month old)


title: Atopic non-wheezer rep14 nasal UNPAIRED

description: Sample name: AF106


title: m3_Aged_C8_new_384

description: Aged (19 month old)


title: Atopic non-wheezer rep13 tracheal

description: Sample name: AF102


title: Atopic non-wheezer rep9 tracheal

description: Sample name: AF068


title: Atopic non-wheezer rep13 nasal

description: Sample name: AF101


title: m3_Young_C10_new_384

description: Young (3 month old)


title: Atopic non-wheezer rep9 nasal

description: Sample name: AF067


title: Atopic non-wheezer rep14 tracheal UNPAIRED

description: Sample name: AF096


title: m3_Aged_D15_new_384

description: Aged (19 month old)


title: m3_Young_G13_new_384

description: Young (3 month old)


title: m3_Aged_C4_new_384

description: Aged (19 month old)


title: m3_Young_I8_new_384

description: Young (3 month old)


title: Atopic non-wheezer rep11 nasal

description: Sample name: AF082


title: Atopic non-wheezer rep8 tracheal

description: Sample name: AF060


title: Atopic non-wheezer rep8 nasal

description: Sample name: AF059


title: Atopic non-wheezer rep7 nasal

description: Sample name: AF051


title: Atopic non-wheezer rep7 tracheal

description: Sample name: AF052


title: m3_Aged_L4_new_384

description: Aged (19 month old)


title: m3_Young_E19_new_384

description: Young (3 month old)


title: m3_Young_C6_new_384

description: Young (3 month old)


title: m3_Young_A20_new_384

description: Young (3 month old)


title: m3_Aged_N12_new_384

description: Aged (19 month old)


title: Atopic non-wheezer rep10 nasal

description: Sample name: AF075


title: Atopic non-wheezer rep6 nasal

description: Sample name: AF043


title: m3_Young_J6_new_384

description: Young (3 month old)


title: Atopic non-wheezer rep5 nasal

description: Sample name: AF035


title: m3_Young_F23_new_384

description: Young (3 month old)


title: m3_Young_E22_new_384

description: Young (3 month old)


title: Atopic non-wheezer rep5 tracheal

description: Sample name: AF036


title: Atopic non-wheezer rep4 nasal

description: Sample name: AF027


title: Atopic non-wheezer rep3 tracheal

description: Sample name: AF020


title: Atopic non-wheezer rep4 tracheal

description: Sample name: AF028


title: m3_Aged_H7_new_384

description: Aged (19 month old)


title: Atopic non-wheezer rep3 nasal

description: Sample name: AF019


title: m3_Aged_B18_new_384

description: Aged (19 month old)


title: m3_Aged_I16_new_384

description: Aged (19 month old)


title: Atopic non-wheezer rep2 tracheal

description: Sample name: AF012


title: m3_Aged_B21_new_384

description: Aged (19 month old)


title: Atopic non-wheezer rep2 nasal

description: Sample name: AF011


title: Atopic non-wheezer rep1 tracheal

description: Sample name: AF004


title: m3_Aged_O17_new_384

description: Aged (19 month old)


title: m3_Aged_E7_new_384

description: Aged (19 month old)


title: m3_Aged_J18_new_384

description: Aged (19 month old)


title: Non-atopic wheezer rep13 nasal UNPAIRED

description: Sample name: AF097

value: Non-atopic asthmatic


title: Atopic non-wheezer rep1 nasal

description: Sample name: AF003


title: m3_Aged_G9_new_384

description: Aged (19 month old)


title: Non-atopic wheezer rep11 nasal UNPAIRED

description: Sample name: AF084


title: Atopic non-wheezer rep6 tracheal

description: Sample name: AF044


title: m3_Aged_C14_new_384

description: Aged (19 month old)


title: m3_Young_L15_new_384

description: Young (3 month old)


title: m3_Aged_D4_new_384

description: Aged (19 month old)


title: Non-atopic wheezer rep10 nasal UNPAIRED

description: Sample name: AF077


title: m3_Aged_J4_new_384

description: Aged (19 month old)


title: Non-atopic wheezer rep9 nasal UNPAIRED

description: Sample name: AF069


title: m3_Young_L12_new_384

description: Young (3 month old)


title: Non-atopic wheezer rep8 tracheal UNPAIRED

description: Sample name: AF070


title: m3_Aged_M6_new_384

description: Aged (19 month old)


title: Non-atopic wheezer rep7 tracheal UNPAIRED

description: Sample name: AF062


title: Non-atopic wheezer rep12 nasal UNPAIRED

description: Sample name: AF091


title: m3_Young_I16_new_384

description: Young (3 month old)


title: Non-atopic wheezer rep7 nasal UNPAIRED

description: Sample name: AF053


title: m3_Young_N16_new_384

description: Young (3 month old)


title: Non-atopic wheezer rep6 tracheal UNPAIRED

description: Sample name: AF054


title: m3_Aged_N14_new_384

description: Aged (19 month old)


title: m3_Young_F22_new_384

description: Young (3 month old)


title: Non-atopic wheezer rep6 nasal UNPAIRED

description: Sample name: AF037


title: m3_Aged_H23_new_384

description: Aged (19 month old)


title: m3_Aged_E20_new_384

description: Aged (19 month old)


title: m3_Young_F20_new_384

description: Young (3 month old)


title: Non-atopic wheezer rep8 nasal UNPAIRED

description: Sample name: AF061


title: Non-atopic wheezer rep3 tracheal

description: Sample name: AF022


title: Non-atopic wheezer rep4 tracheal

description: Sample name: AF030


title: m3_Aged_O6_new_384

description: Aged (19 month old)


title: Non-atopic wheezer rep4 nasal

description: Sample name: AF029


title: m3_Aged_C13_new_384

description: Aged (19 month old)


title: Non-atopic wheezer rep5 tracheal

description: Sample name: AF046


title: m3_Young_L14_new_384

description: Young (3 month old)


title: m3_Aged_F2_new_384

description: Aged (19 month old)


title: Non-atopic wheezer rep2 nasal

description: Sample name: AF013


title: Non-atopic wheezer rep1 tracheal

description: Sample name: AF006


title: Non-atopic wheezer rep2 tracheal

description: Sample name: AF014


title: m3_Aged_F19_new_384

description: Aged (19 month old)


title: m3_Aged_H3_new_384

description: Aged (19 month old)


title: Non-atopic wheezer rep3 nasal

description: Sample name: AF021


title: Non-atopic non-wheezer rep15 tracheal UNPAIRED

description: Sample name: AF108

value: Non-atopic non-asthmatic


title: Non-atopic wheezer rep5 nasal

description: Sample name: AF045


title: Non-atopic non-wheezer rep14 tracheal UNPAIRED

description: Sample name: AF094


title: Non-atopic non-wheezer rep13 nasal

description: Sample name: AF104


title: m3_Aged_J14_new_384

description: Aged (19 month old)


title: Non-atopic non-wheezer rep14 nasal UNPAIRED

description: Sample name: AF080


title: Non-atopic non-wheezer rep12 tracheal

description: Sample name: AF100


title: m3_Aged_L16_new_384

description: Aged (19 month old)


title: Non-atopic non-wheezer rep13 tracheal

description: Sample name: AF105


title: m3_Young_H21_new_384

description: Young (3 month old)


title: m3_Aged_E12_new_384

description: Aged (19 month old)


title: m3_Aged_H22_new_384

description: Aged (19 month old)


title: Non-atopic non-wheezer rep12 nasal

description: Sample name: AF099


title: m3_Aged_B11_new_384

description: Aged (19 month old)


title: Non-atopic non-wheezer rep11 tracheal

description: Sample name: AF088


title: Non-atopic non-wheezer rep10 tracheal

description: Sample name: AF074


title: Non-atopic non-wheezer rep11 nasal

description: Sample name: AF087


title: m3_Young_A21_new_384

description: Young (3 month old)


title: m3_Young_A23_new_384

description: Young (3 month old)


title: m3_Aged_A14_new_384

description: Aged (19 month old)


title: Non-atopic wheezer rep1 nasal

description: Sample name: AF005


title: Non-atopic non-wheezer rep9 tracheal

description: Sample name: AF066


title: m3_Young_N22_new_384

description: Young (3 month old)


title: m3_Young_K12_new_384

description: Young (3 month old)


title: m3_Young_L5_new_384

description: Young (3 month old)


title: Non-atopic non-wheezer rep10 nasal

description: Sample name: AF073


title: m3_Young_N14_new_384

description: Young (3 month old)


title: Non-atopic non-wheezer rep9 nasal

description: Sample name: AF065


title: Non-atopic non-wheezer rep8 tracheal

description: Sample name: AF058


title: m3_Young_I15_new_384

description: Young (3 month old)


title: Non-atopic non-wheezer rep8 nasal

description: Sample name: AF057


title: m3_Aged_E22_new_384

description: Aged (19 month old)


title: m3_Aged_I12_new_384

description: Aged (19 month old)


title: m3_Young_N13_new_384

description: Young (3 month old)


title: OreR_Input_rep3

city: Princeton

first: Stanislav

middle: Y

last: Shvartsman

state: NJ

address: Carl Icahn Lab, Washington Road

department: Chemical and Biological Engineering

institute: Princeton University/ Lewis-Sigler Institute for Integrative Genomics

library_source: genomic

data_processing:
Reads were trimmed for adaptors using Galaxy: TrimGalore!
Reads were mapped to the drosophila dm6 genome using Galaxy: BWA-MEM, Version: 0.7.17-r1188, -t 6 -v 1
SAMtools view (Galaxy Version 1.9) was used to remove reads with a quality score lower than 20.
PicardMarkDuplicates (Galaxy version  2.18.2.1) was used to remove duplicates from each sample
Replicate BAM files were merged into a single BAM files using SAMtools
Peaks were called using MACS2 (Zhang et al. 2008), parameters: -q 0.01 --keep-dup all –nomodel –extsize 145.  Peak calling control was OreR ChIP replicates.  we only considered peaks with a log2(fold change) of 5 or greater
Other analysis was done using several R packages including: GenomicRanges and ChIPSeeker.  Many peaks called by MACS2 had low read counts and we did not trust these in our analysis as true Cic targets.  Therefore, we only considered peaks with a log2(fold change) of 5 or greater.  Additionally, we only kept peaks in chromosomes: 2R, 2L, 3R, 3L, 4, and X.  Lastly, we removed peaks found in the dsor1 and rl coding regions as our lab consistently clones with these genes, and they were suspected to be contamination.
Genome_build: dm6
Supplementary_files_format_and_content: bigWig files were generated using R.  Coverage of reads across 10 bp windows of the genome were determined using the coverage function in the GenomicRanges package. Scores represent counts per million total read counts in that condition

molecule: genomic DNA

organism: Drosophila melanogaster

source_name: Drosophila melanogaster embryo

growth_protocol: Embryos were collected for 3-4 hours in the dark.  After fixation, embryos that were at NC 14 were selected from the general pool.  Stage was determined based on nuclear density and membrane length

extract_protocol:
Embryos were sonicated 7 times, 20 seconds each, at 20% output.  Cic-DNA complexes were isolated using magnetic protein G dynabead and GFP antibody.  DNA was uncrosslinked from protein and purified.
Purified ChIP and input DNA were prepared for Illumina sequencing using the NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645S).  The primers used for multiplexing the library came from the NEBNext Multiplex Oligos for Illumina Kit (E7335S).

treatment_protocol: Embryos were dechorionated in bleach in red light (keeps optogenetic constructs turned off) for 2 minutes.  Embryos were exposed to blue light for varying lengths of time (dependent on condition).  They were then fixed in a mixture of paraformaldehyde, PBS/0.5% TritonX-100, and heptane for 15 minutes

value: embryo

tag: chip-antibody

tag: embryonic stage

value: early nuclear cycle 14


title: m3_Aged_B6_new_384

description: Aged (19 month old)


title: Non-atopic non-wheezer rep7 tracheal

description: Sample name: AF050


title: m3_Aged_D17_new_384

description: Aged (19 month old)


title: m3_Young_C4_new_384

description: Young (3 month old)


title: OreR_Input_rep1


title: m3_Aged_M7_new_384

description: Aged (19 month old)


title: OreR_ChIP_rep2

value: GFP (Millipore, AB3080)


title: m3_Aged_B16_new_384

description: Aged (19 month old)


title: m3_Aged_I6_new_384

description: Aged (19 month old)


title: Non-atopic non-wheezer rep7 nasal

description: Sample name: AF049


title: Non-atopic non-wheezer rep6 nasal

description: Sample name: AF041


title: Non-atopic non-wheezer rep6 tracheal

description: Sample name: AF042


title: OreR_ChIP_rep3


title: m3_Young_E6_new_384

description: Young (3 month old)


title: m3_Aged_E3_new_384

description: Aged (19 month old)


title: m3_Aged_P22_new_384

description: Aged (19 month old)


title: OreR_ChIP_rep1


title: Non-atopic non-wheezer rep5 nasal

description: Sample name: AF033


title: m3_Aged_P8_new_384

description: Aged (19 month old)


title: m3_Young_N7_new_384

description: Young (3 month old)


title: Non-atopic non-wheezer rep5 tracheal

description: Sample name: AF034


title: Non-atopic non-wheezer rep4 tracheal

description: Sample name: AF026


title: m3_Young_C17_new_384

description: Young (3 month old)


title: OreR_Input_rep2


title: m3_Aged_K5_new_384

description: Aged (19 month old)


title: CicsfGFP_TorD_Input_rep1


title: CicsfGFP_TorD_ChIP_rep3


title: CicsfGFP_TorD_Input_rep2


title: m3_Aged_H18_new_384

description: Aged (19 month old)


title: m3_Aged_P19_new_384

description: Aged (19 month old)


title: CicsfGFP_TorD_Input_rep3


title: m3_Young_H16_new_384

description: Young (3 month old)


title: Non-atopic non-wheezer rep3 tracheal

description: Sample name: AF018


title: Non-atopic non-wheezer rep3 nasal

description: Sample name: AF017


title: m3_Young_O10_new_384

description: Young (3 month old)


title: m3_Young_P6_new_384

description: Young (3 month old)


title: Non-atopic non-wheezer rep2 tracheal

description: Sample name: AF010


title: Non-atopic non-wheezer rep4 nasal

description: Sample name: AF025


title: CicsfGFP_TorD_ChIP_rep2


title: m3_Young_N5_new_384

description: Young (3 month old)


title: CicsfGFP_OptoSOS_30min_Input_rep3

description: The Gal4 used in this study was MTD gal4 (maternal triple driver).


title: CicsfGFP_TorD_ChIP_rep1


title: m3_Young_K5_new_384

description: Young (3 month old)


title: m3_Young_J10_new_384

description: Young (3 month old)


title: CicsfGFP_OptoSOS_30min_Input_rep2


title: Non-atopic non-wheezer rep2 nasal

description: Sample name: AF009


title: m3_Aged_E15_new_384

description: Aged (19 month old)


title: m3_Aged_F21_new_384

description: Aged (19 month old)


title: CicsfGFP_OptoSOS_30min_Input_rep1


title: m3_Young_I17_new_384

description: Young (3 month old)


title: Non-atopic non-wheezer rep1 tracheal

description: Sample name: AF002


title: CicsfGFP_OptoSOS_30min_ChIP_rep3


title: m3_Aged_M22_new_384

description: Aged (19 month old)


title: m3_Young_F21_new_384

description: Young (3 month old)


title: CicsfGFP_OptoSOS_30min_ChIP_rep2


title: m3_Aged_H19_new_384

description: Aged (19 month old)


title: m3_Aged_E23_new_384

description: Aged (19 month old)


title: m3_Aged_N20_new_384

description: Aged (19 month old)


title: m3_Aged_N18_new_384

description: Aged (19 month old)


title: m3_Young_G19_new_384

description: Young (3 month old)


title: Non-atopic non-wheezer rep1 nasal

description: Sample name: AF001


title: m3_Aged_D20_new_384

description: Aged (19 month old)


title: CicsfGFP_OptoSOS_5min_Input_rep1


title: m3_Young_N20_new_384

description: Young (3 month old)


title: dental mesenchyme, CKO12638

city: Los Angeles

first: Shuo

state: California

address: 2250 Alcazar Street, Clinical Science Annex, Room 103, CLINICAL SCIENCE ANNEX ROOM 103

institute: Center for Craniofacial Molecular Biology, University of Southern California

data_processing:
Illumina Casava1.8 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome and transcriptome using STAR 2.6.1d, and normalized using Upper quartile.
Lognormal with shrinkage mean value were calculated using Gene-specific analysis by Partek flow (Partek Inc., USA).
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include lognormal with shrinkage mean value for each feature in individual sample.

source_name: Incisor

growth_protocol: Mice were housed in pathogen-free conditions.

extract_protocol:
The proximal region of the incisor was collected for RNA isolation with an RNeasy Micro Kit (QIAGEN).
The quality of RNA samples was evaluated using an Agilent 2100 Bioanalyzer and all samples used for sequencing had RNA integrity (RIN) numbers >9.0. cDNA library preparation and sequencing were performed by DNA Link, Inc. Single-end reads with 75 cycles were performed on NextSeq500 for three pairs of samples.

treatment_protocol: Tamoxifen was dissolved in corn oil  at 20 mg/ml and injected intraperitoneally at a dosage of 1.5mg/10g body weight daily for 3 consecutive days at the age of 1 month old.

tag: age

value: 5-week old

value: dental mesenchyme


title: dental mesenchyme, CKO12636


title: dental mesenchyme, WT12635


title: m3_Aged_D6_new_384

description: Aged (19 month old)


title: dental mesenchyme, WT12632


title: dental mesenchyme, CKO12637


title: m3_Young_D19_new_384

description: Young (3 month old)


title: m3_Aged_J17_new_384

description: Aged (19 month old)


title: m3_Aged_O19_new_384

description: Aged (19 month old)


title: m3_Aged_G20_new_384

description: Aged (19 month old)


title: CicsfGFP_OptoSOS_30min_ChIP_rep1


title: m3_Aged_N11_new_384

description: Aged (19 month old)


title: m3_Young_L18_new_384

description: Young (3 month old)


title: m3_Aged_O8_new_384

description: Aged (19 month old)


title: CicsfGFP_OptoSOS_5min_Input_rep3


title: dental mesenchyme, WT12639


title: m3_Young_B9_new_384

description: Young (3 month old)


title: m3_Young_E12_new_384

description: Young (3 month old)


title: CicsfGFP_OptoSOS_5min_ChIP_rep3


title: CicsfGFP_OptoSOS_5min_Input_rep2


title: m3_Aged_B23_new_384

description: Aged (19 month old)


title: CicsfGFP_OptoSOS_1min_Input_rep2


title: m3_Aged_E11_new_384

description: Aged (19 month old)


title: m3_Aged_K6_new_384

description: Aged (19 month old)


title: m3_Young_A12_new_384

description: Young (3 month old)


title: m3_Aged_F5_new_384

description: Aged (19 month old)


title: m3_Aged_B17_new_384

description: Aged (19 month old)


title: m3_Aged_L17_new_384

description: Aged (19 month old)


title: ORGN-Lgr5-plate10-labeled

city: Utrecht

first: Nico

last: Battich

state: Utrecht

address: Uppsalalaan 8

country: Netherlands

institute: Hubrecht Institute

description: 5-ethymyluridin labeling

data_processing:
Library strategy: scEU-Seq
Read 2 of Sequencing liberties were using STAR 2.5 with default parameters. If the length of the poly-T track in read 1 was less than 19 bases, the read was discarded.
Genome_build: ensemble release 90 of the homo sapiens GRCh38 genome, extended with ERCC92 spike-ins and ensemble release 90 of the mus musculus GRCm38 genome, extended with ERCC92 spike-ins
Supplementary_files_format_and_content: The number of UMI count per gene was obtained by pooling all reads mapping to the same gene and having the same cell barcode as previously (Grün, Dominic, Lennart Kester, and Alexander Van Oudenaarden. "Validation of noise models for single-cell transcriptomics." Nature methods 11.6 (2014): 637) using only uniquely mapped reads. The minimal number of UMI detected in a cell for the RPE1-FUCCI cell-cycle analysis was of 2,300, while the threshold used for murine in intestinal organoid cells was of 1,000. The UMI for a given gene in a cell was considered to be unspliced if at least one base of any read belonging to that UMI mapped outside of the exons of the gene.

source_name: Intestinal organoids treated with DSMO for 120 min, with EU for 120 minutes, or EU for ~22 hours with a U chase 0, 45 or 360 minutes

growth_protocol: RPE1-FUCCI cells were cultured in Dulbecco's modified Eagle's medium (DMEM)/F12, supplemented with GlutaMAX (Gibco), FBS (Gibco), and penicillin/streptomycin (Gibco), following standard procedures. Organoids were expanded in medium termed ENR, consisting of Advanced Dulbecco’s modified Eagle’s medium/F12 (Advanced DMEM) with HEPES (10mM, Sigma), penicillin/streptomycin (1x, Thermofisher) and Glutamax. Advanced DMEM was supplemented with 1x B27 (Thermofisher), 1 mM N-acetylcysteine (Sigma), 50 ng/ml murine recombinant epidermal growth factor (PeproTech), R-spondin 1 conditioned medium (5% of final volume) and Noggin conditioned medium (5% of final volume) to generate ENR medium. To generate conditioned media, HEK293T cells were stably transfected with Rspo1-Fc (gift from Calvin Kuo, Stanford University) or transient transfection with mouse Noggin-Fc expression vector and grown for 1 week in Advanced DMEM supplemented with penicillin/streptomycin, and Glutamax. Organoids were plated in Reduced Growth Factor Basement Membrane Matrix (BME) Type 2 (Trevigen)

extract_protocol:
After incubation with EU, cells were dissociated, fixed with paraformaldehyde, and azide-biotin was clicked in situ after cell permeabilization to mark EU-labeled RNA. We then sorted single cells in 384-well plates and performed first strand synthesis with poly-T primers containing a cell barcode, a unique molecular identifier, a 5’ sequencing adapter, and the T7 promoter. Cells were pooled and streptavidin magnetic beads were used to separate EU labeled and unlabeled mRNA/cDNA hybrids. Library preparation was performed for both fractions separately.
Runs contain the 6-nucleotide long UMI in the first nucleotides of Read 1, followed by the 8-nucleotide long cell-specific barcode. Illumina TruSeq adapaters

treatment_protocol: Cells were incubated with the EU and/or DMSO for the desired time (e.g. 60 min). The final concentration of EU was 200 µM for RPE1-FUCCI chase experiments, 400 µM for RPE1-FUCCI pulse experiments, 166 µM for chase experiments in organoids and 1.6 mM for pulse experiments in Organoids. The U chase phase of the was done with 400 µM U. Cells were dissociated using TrypLE enzyme mix (Gibco) and mechanical sheering if required.

value: Murine intestinal organoids, Lgr5DTR-eGFP line

tag: genetic tag


title: m3_Young_O20_new_384

description: Young (3 month old)


title: ORGN-Lgr5-plate14-labeled

description: 5-ethymyluridin labeling


title: m3_Aged_A21_new_384

description: Aged (19 month old)


title: CicsfGFP_OptoSOS_5min_ChIP_rep2


title: CicsfGFP_OptoSOS_1min_ChIP_rep2


title: ORGN-Lgr5-plate12-labeled

description: 5-ethymyluridin labeling


title: CicsfGFP_OptoSOS_5min_ChIP_rep1


title: ORGN-Lgr5-plate13-labeled

description: 5-ethymyluridin labeling


title: CicsfGFP_OptoSOS_1min_ChIP_rep1


title: CicsfGFP_OptoSOS_NoLight_Input_rep3


title: CicsfGFP_OptoSOS_1min_Input_rep1


title: ORGN-Lgr5-plate11-labeled

description: 5-ethymyluridin labeling


title: m3_Young_D7_new_384

description: Young (3 month old)


title: CicsfGFP_OptoSOS_NoLight_Input_rep2


title: m3_Young_D22_new_384

description: Young (3 month old)


title: m3_Young_O22_new_384

description: Young (3 month old)


title: ORGN-Lgr5-plate05-labeled

description: 5-ethymyluridin labeling


title: ORGN-Lgr5-plate09-labeled

description: 5-ethymyluridin labeling


title: CicsfGFP_OptoSOS_NoLight_ChIP_rep2


title: m3_Young_O13_new_384

description: Young (3 month old)


title: m3_Aged_C6_new_384

description: Aged (19 month old)


title: ORGN-Lgr5-plate07-labeled

description: 5-ethymyluridin labeling


title: CicsfGFP_OptoSOS_NoLight_Input_rep1


title: ORGN-Lgr5-plate04-labeled

description: 5-ethymyluridin labeling


title: m3_Aged_I14_new_384

description: Aged (19 month old)


title: m3_Young_E23_new_384

description: Young (3 month old)


title: m3_Young_A17_new_384

description: Young (3 month old)


title: ORGN-Lgr5-plate03-labeled

description: 5-ethymyluridin labeling


title: CicsfGFP_OptoSOS_NoLight_ChIP_rep3


title: ORGN-Lgr5-plate08-labeled

description: 5-ethymyluridin labeling


title: m3_Aged_C2_new_384

description: Aged (19 month old)


title: m3_Aged_F10_new_384

description: Aged (19 month old)


title: m3_Aged_D9_new_384

description: Aged (19 month old)


title: m3_Young_N21_new_384

description: Young (3 month old)


title: m3_Young_P9_new_384

description: Young (3 month old)


title: m3_Aged_C23_new_384

description: Aged (19 month old)


title: CicsfGFP_OptoSOS_NoLight_ChIP_rep1


title: ORGN-Lgr5-plate06-labeled

description: 5-ethymyluridin labeling


title: m3_Young_E4_new_384

description: Young (3 month old)


title: m3_Aged_E10_new_384

description: Aged (19 month old)


title: m3_Aged_H15_new_384

description: Aged (19 month old)


title: ORGN-Lgr5-plate13-unlabeled

description: 5-ethymyluridin labeling


title: ORGN-Lgr5-plate02-labeled

description: 5-ethymyluridin labeling


title: m3_Young_B18_new_384

description: Young (3 month old)


title: CicsfGFP_Input_rep2


title: ORGN-Lgr5-plate01-labeled

description: 5-ethymyluridin labeling


title: m3_Aged_F13_new_384

description: Aged (19 month old)


title: CicsfGFP_Input_rep3


title: m3_Aged_C21_new_384

description: Aged (19 month old)


title: m3_Young_B16_new_384

description: Young (3 month old)


title: CicsfGFP_Input_rep1


title: CicsfGFP_ChIP_rep3


title: m3_Aged_P14_new_384

description: Aged (19 month old)


title: ORGN-Lgr5-plate14-unlabeled

description: 5-ethymyluridin labeling


title: ORGN-Lgr5-plate12-unlabeled

description: 5-ethymyluridin labeling


title: ORGN-Lgr5-plate11-unlabeled

description: 5-ethymyluridin labeling


title: CicsfGFP_ChIP_rep2


title: m3_Aged_K19_new_384

description: Aged (19 month old)


title: m3_Young_B17_new_384

description: Young (3 month old)