title: 10_21scP0

city: La Jolla

first: Tatiana

last: Kisseleva

address: 9500 Gilman Dr

department: School of Medicine

institute: UCSD

data_processing:
Base-calling was performed using Illumina Real Time Analysis RTA 1.18.64 with default parameters
The single-end reads that passed Illumina filters were filtered for reads aligning to tRNA, rRNA, adapter sequences, and spike-in controls. The reads were then aligned to hg19 reference genome using TopHat (v 1.4.1).  DUST scores were calculated with PRINSEQ Lite (v 0.20.3) and low-complexity reads (DUST > 4) were removed from the BAM files.  The alignment results were parsed via the SAMtools to generate SAM files. Read counts to each genomic feature were obtained with the htseq-count program (v 0.6.0)  using the “union” option. After removing absent features (zero counts in all samples), the raw counts were then imported to R/Bioconductor package DESeq2 (v 1.6.3) to identify differentially expressed genes among samples.
Genome_build: hg19
Supplementary_files_format_and_content: DESeq normalized

source_name: primary human HSCs

growth_protocol: Primary human HSCs were cultured in Dulbecco’s modified Eagle’s medium (DMEM Life Technologies, 11965-092) supplemented with 10% FBS (Corning, 35-011-CV) and 1% Antibiotic-Antymycotic (Life Technologies #15240-062).

extract_protocol:
For RNA-seq Total RNA was isolated using Purelink RNA Mini Kit (Life Technologies)
For RNA-seq Illumina Stranded Total RNA (Illumina 20020597)

treatment_protocol: Primary hHSCs (0.15 x 106 cells/well) were cultured in DMEM (Life Technologies) + 10% FBS (Corning) + 1% Antibiotic-Antiymycotic (Life Technologies), then serum starved for 24 h, and stimulated ± TGFβ1 (5 ng/ml, 24 h), TGF-βRI inhibitor SB431542 (10µM), IL-6 (10ng/ml), PDGF (10ng/ml) for 24 h, and harvested in TRIzol for RNA extraction

tag: cell type

value: Hepatic stellate cells isolated from human liver

tag: disease stage

value: ALD


title: 6_14scP0

status: Public on Apr 10 2020

type: SRA

country: USA

library_source: transcriptomic

molecule: polyA RNA

organism: Homo sapiens


title: 14_26scP1

value: Normal


title: 9_21scP0


title: 15_34scP0


title: 11_23scP0


title: 12_23scP0


title: AR_VCAP_Control_Rep1

city: Berlin

first: Bernard

last: Haendler

address: Muellerstrasse 178

department: Preclinical Research

country: Germany

institute: Bayer AG

library_source: genomic

data_processing:
Illumina bcl2fastq v2.17 was used to convert bcl to fastqs.
Sequencing reads were mapped to human genome hg19 using the BWA aligner with default settings.
Duplicate reads were marked with Picard tools and filtered out together with multi-mapping reads (MAPQ score below 30).
Narrow peaks were called by MACS2 with default parameters and a q-value cutoff of 0.05.
Human genome blacklisted regions (ENCODE consortium) were excluded.
Genome_build: hg19
Supplementary_files_format_and_content: narrowpeak files with genome regions

molecule: genomic DNA

source_name: VCaP (prostate)

growth_protocol: Cells were routinely grown in the recommended medium in the presence of 10% (v/v) fetal bovine serum (Life Technologies, Carlsbad, CA) at 37°C and 5% CO2. Authentication was performed by the DSMZ using short tandem repeat DNA typing analysis.

extract_protocol:
For chromatin immunoprecipitation experiments with subsequent sequencing (ChIP-seq), three replicates of eight million cells were seeded in 15-cm plates and treated with 2 µM darolutamide plus 1 nM R1881 for 22 h, subsequently fixed for ten minutes with 1% formaldehyde and processed as described previously (Baumgart et al. Nucleic Acids Res. 2017 Jul 27;45(13):7722-7735)
Library preparation was done as described by the manufacturer (MicroPlex Library Preparation Kit v2; Diagenode SA, Seraing, Belgium). The libraries were sequenced on a HiSeq2500 Illumina machine with 50 base pair, single-end reads to an average depth of 25–30 million reads per sample.

treatment_protocol: Cells were stimulated with the synthetic androgen R1881 at a concentration of 1 nM after two days of starvation in medium supplemented with 10% charcoal-stripped fetal calf serum (cFCS). Darolutamide was added at a final concentration of 2 µM and the cells were harvested 22 h post-treatment.

tag: chip antibody

value: AR

tag: cell line

value: VCaP

tag: treatment

value: Control


title: AR_VCAP_Control_Rep3


title: AR_VCAP_Daro_Rep2

value: Darolutamide


title: 13_26scP1


title: AR_VCAP_Control_Rep2


title: 5_14scP0


title: 4_13scP0


title: 3_13scP0


title: 2_02scp2


title: My-La_HiChIP

city: Manchester

first: Paul

last: Martin

state: Greater Manchester

address: Oxford Road

department: Centre for Genetics and Genomics Versus Arthritis

country: United Kingdom

institute: Univeristy of Manchester

description:
This Sample represents 1 replicate
HiChIP, My-La CD8+ T lymphocyte cells, replicate 1

data_processing:
Library strategy: HiChIP
CHi-C: Fastq files were filtered and mapped to the hg19 genome by the Hi-C User Pipeline (HiCUP) v0.5.8. The filtered files for two biological replicates were simultaneously run through the Capture Hi-C Analysis of Genomic Organisation (CHiCAGO) v1.1.8, with a score threshold of 5. Interactions were limited to same-chromosome (cis) and to psoriasis-specific loci.
Hi-C: Fastq files were filtered and adapters removed using fastp v0.19.4. Reads were mapped to the GRCh38 genome with Hi-C Pro v2.11.0 using default settings. Hi-C interaction matrices were normalised within Hi-C Pro using iterative correction and eigenvector decomposition (ICE). Topologically associating domains (TADs) were then called in TADtool software using insulation score with the normalised Hi-C contact matrices, binned with 40 kb resolution. For comparison, TADs were lifted over to the hg19 genome using UCSC LiftOver.
Hi-ChIP: Fastq files were filtered and the adapters were removed using fastp v0.19.4. The reads were then mapped to the GRCh38 genome with Hi-C Pro v2.11.0, using default settings. Genome-wide anchors for H3K27ac were called in hichipper v0.7.3 using the self-circle and dangling end reads for the first replicate, extended for 147 bp. For interactions, two replicates were merged and loops were identified using FitHiChIP using the following settings: Coverage normalisation, stringent background with merging enabled, peaks generated from HiChIP data using the included tool and 5kb bin size.
RNA-seq: Reads were quality trimmed using Trimmomatic v0.38 using a sliding window of 5 with a mean minimum quality of 20. Adapters and poly A/poly G tails were removed using Cutadapt v1.18 and then UMIs were extracted from the 5’ of the reads using UMI-tools v0.5.5. Reads were then mapped using STAR v2.5.3a on the GRCh38 genome with GENCODE annotation v29. Reads were de-duplicated using UMIs with UMI-tools and then counted using HTSeq v0.11.2. Count matrixes were analysed in R 3.5.1 and normalisation and differential expression analysis was conducted using DESeq2 v1.22.2.
Genome_build: hg19
Supplementary_files_format_and_content: TADs.bed.gz: TAD coordinates (chromosome, start, end)
Supplementary_files_format_and_content: Ps_bait_fragments.bed.gz: HindIII fragments tagged by psoriasis-associated GWAS SNPs (chromsosome, start, end, SNP)
Supplementary_files_format_and_content: CHi-C_interactions.txt.gz: Significant CHi-C interacting HindIII fragments involving psoriasis loci (bait chromosome, bait start, bait end, other-end chromosome, other-end start, other-end end, CHiCAGO score, SNP)
Supplementary_files_format_and_content: HiChIP_9q31_interactions.txt.gz: Significant HiChIP interactions originating from the H3K27ac peak at KLF4 in 9q31 (peak chromosome, peak start, peak end, other-end chromosome, other-end start, other-end end, Hichipper score)
Supplementary_files_format_and_content: combined_RNA-seq_counts.csv: Raw RNA-seq counts for all experimental samples
Supplementary_files_format_and_content: 27ac.anchors.bed.gz: Standard bed files
Supplementary_files_format_and_content: HiChIP_interactions.txt.gz: FitHiChIP interaction files (with header)

source_name: My-La CD8+ T lymphocyte cells

growth_protocol: My-La cells were cultured in RPMI 1640 medium supplemented with 10% AB human serum, 100 U/mL recombinant human IL-2 and 1% penicillin-streptomycin in 25cm2 vented culture flasks at 37C/5% CO2

extract_protocol:
Cells were thawed on ice and re-suspended in 50ml ice-cold lysis buffer (10mM Tris-HCl pH 8, 10mM NaCl, 0.2% Igepal CA-630, one protease inhibitor cocktail tablet). Cells were lysed for 30 minutes at 4C with rotation. The nuclei were permeabilised and the chromatin was digested with MboI. Ends were marked with biotin followed by in-nucleus ligation, shearing by sonication and immunoprecipitation with an antibody for H3K27ac (Abcam ab4729). De-crosslinking, biotin pulldown and tagmentation was conducted according to the previously established HiChIP protocol (Mumbach et al., 2016)
Sequencing library was generated with 10 cycles of PCR using Nextera dual index primers and Phusion HF 2X (NEB)

treatment_protocol: Cells were fixed in 1% formaldehyde for 10 minutes at room temperature, Fixation was quenched by the addition of cold 1M glycine to a final concentration of 0.125M. Pelleted cells were frozen on dry ice and stored at -80C.

tag: tissue

value: My-La CD8+ T lymphocyte cell line

tag: stimulation

tag: biomaterial provider

tag: restriction enzyme

value: MboI


title: control_7096

city: Ramat Gan

first: Tzipi

last: Braun

address: Derech Sheba 2

country: Israel

institute: Sheba Medical Center at Tel Hashomer

data_processing:
Reads were quantified by kallisto version 42.5, using Gencode v24 as the reference genome
Estimated counts were normalized to Reads per Million (RPM) as an output.
Data was filtered to includ 14,778 protein-coding mRNA genes with RPM above 3 in 20% of the samples.
Genome_build: Gencode v24
Supplementary_files_format_and_content: tab-delimited text files include RPM values for each Sample

molecule: total RNA

source_name: Duodenum FFPE

extract_protocol:
RNA was isolated from formalin-fixed, paraffin-embedded (FFPE) duodenal biopsies tissue sections obtained during diagnostic endoscopy using the Qiagen AllPrep RNA/DNA FFPE Kit
Lexogen QuantSeq 3' mRNA-Seq libraries and single-end was performed

treatment_protocol: Archived stored formalin-fixed paraffin-embedded (FFPE) tissue was used

tag: diagosis

value: control

tag: age

tag: gender

value: M

value: normal

tag: group

value: training

tag: location

value: Duodenum


title: 16_34scP0


title: control_7095

value: F


title: AR_VCAP_Daro_Rep1


title: control_7091


title: HaCaT_unstimulated_HiChIP

description:
This Sample represents 1 replicate
HiChIP, HaCaT Keratinocyte cells, unstimulated, replicate 1

source_name: HaCaT Keratinocyte cells

growth_protocol: HaCaT cells were cultured in high glucose DMEM supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin in 75cm2 vented culture flasks at 37C/5% CO2

value: HaCaT Keratinocyte cell line


title: control_7092

value: abnormal


title: My-La_HiC

description:
This Sample represents 1 replicate
Hi-C, My-La CD8+ T lymphocyte cells, replicate 1

data_processing:
CHi-C: Fastq files were filtered and mapped to the hg19 genome by the Hi-C User Pipeline (HiCUP) v0.5.8. The filtered files for two biological replicates were simultaneously run through the Capture Hi-C Analysis of Genomic Organisation (CHiCAGO) v1.1.8, with a score threshold of 5. Interactions were limited to same-chromosome (cis) and to psoriasis-specific loci.
Hi-C: Fastq files were filtered and adapters removed using fastp v0.19.4. Reads were mapped to the GRCh38 genome with Hi-C Pro v2.11.0 using default settings. Hi-C interaction matrices were normalised within Hi-C Pro using iterative correction and eigenvector decomposition (ICE). Topologically associating domains (TADs) were then called in TADtool software using insulation score with the normalised Hi-C contact matrices, binned with 40 kb resolution. For comparison, TADs were lifted over to the hg19 genome using UCSC LiftOver.
Hi-ChIP: Fastq files were filtered and the adapters were removed using fastp v0.19.4. The reads were then mapped to the GRCh38 genome with Hi-C Pro v2.11.0, using default settings. Genome-wide anchors for H3K27ac were called in hichipper v0.7.3 using the self-circle and dangling end reads for the first replicate, extended for 147 bp. For interactions, two replicates were merged and loops were identified using FitHiChIP using the following settings: Coverage normalisation, stringent background with merging enabled, peaks generated from HiChIP data using the included tool and 5kb bin size.
RNA-seq: Reads were quality trimmed using Trimmomatic v0.38 using a sliding window of 5 with a mean minimum quality of 20. Adapters and poly A/poly G tails were removed using Cutadapt v1.18 and then UMIs were extracted from the 5’ of the reads using UMI-tools v0.5.5. Reads were then mapped using STAR v2.5.3a on the GRCh38 genome with GENCODE annotation v29. Reads were de-duplicated using UMIs with UMI-tools and then counted using HTSeq v0.11.2. Count matrixes were analysed in R 3.5.1 and normalisation and differential expression analysis was conducted using DESeq2 v1.22.2.
Genome_build: hg19
Supplementary_files_format_and_content: TADs.bed.gz: TAD coordinates (chromosome, start, end)
Supplementary_files_format_and_content: Ps_bait_fragments.bed.gz: HindIII fragments tagged by psoriasis-associated GWAS SNPs (chromsosome, start, end, SNP)
Supplementary_files_format_and_content: CHi-C_interactions.txt.gz: Significant CHi-C interacting HindIII fragments involving psoriasis loci (bait chromosome, bait start, bait end, other-end chromosome, other-end start, other-end end, CHiCAGO score, SNP)
Supplementary_files_format_and_content: HiChIP_9q31_interactions.txt.gz: Significant HiChIP interactions originating from the H3K27ac peak at KLF4 in 9q31 (peak chromosome, peak start, peak end, other-end chromosome, other-end start, other-end end, Hichipper score)
Supplementary_files_format_and_content: combined_RNA-seq_counts.csv: Raw RNA-seq counts for all experimental samples
Supplementary_files_format_and_content: 27ac.anchors.bed.gz: Standard bed files
Supplementary_files_format_and_content: HiChIP_interactions.txt.gz: FitHiChIP interaction files (with header)

extract_protocol:
Cells were thawed on ice and re-suspended in 50ml ice-cold lysis buffer (10mM Tris-HCl pH 8, 10mM NaCl, 0.2% Igepal CA-630, one protease inhibitor cocktail tablet). Cells were lysed on ice for a total of 30 min, with 2x10 strokes of a dounce homogeniser with a 5 minute break between douncing. The nuclei were pelleted and permeabilised and the chromatin was digested with HindIII. Ends were marked with biotin followed by in-nucleus ligation, decrosslinking and biotin pulldown according to the previously established Hi-C protocol (Dryden et al., 2014; P. Martin et al., 2015)
Sequencing libraries were generated with 6-8 cycles of PCR using Truseq single indexes (Illumina) and Phusion polymerase (NEB)

treatment_protocol: Cells were fixed in 2% formaldehyde for 10 minutes at room temperature, Fixation was quenched by the addition of cold 1M glycine to a final concentration of 0.125M. Pelleted cells were frozen on dry ice and stored at -80C.

value: HindIII


title: HaCaT_stimulated_HiChIP

description:
This Sample represents 1 replicate
HiChIP, HaCaT Keratinocyte cells, stimulated, replicate 1

growth_protocol: HaCaT cells were cultured in high glucose DMEM supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin in 75cm2 vented culture flasks at 37C/5% CO2. Cells were stimulated with 100 ng/mL recombinant human IFN-γ (285-IF-100; R&D Systems) for 8 hours.

value: 100 ng/mL recombinant human IFN-γ for 8 hours


title: control_7093


title: control_7090


title: control_7089


title: control_7087

value: validation


title: control_7084


title: control_7085


title: control_7024


title: control_7086


title: celiac_7094

value: celiac


title: control_7018


title: 1_02scp2

value: Hepatic stellate cells isolated from human liver

value: Normal


title: control_7023


title: control_7083


title: control_7013


title: control_7017


title: control_7015


title: control_7016


title: control_7014


title: celiac_7077


title: celiac_7079


title: celiac_7078


title: celiac_7080


title: celiac_7076


title: celiac_7074


title: celiac_7081


title: celiac_7082


title: control_7088


title: celiac_7072


title: celiac_7070


title: celiac_7073


title: celiac_7069


title: celiac_7066


title: celiac_7067


title: celiac_7065


title: celiac_7064


title: celiac_7022


title: celiac_7063


title: celiac_7058


title: celiac_7021


title: celiac_7020


title: celiac_7019


title: celiac_7012


title: celiac_7075


title: celiac_7009


title: celiac_7011


title: celiac_7002


title: celiac_7004


title: celiac_7071


title: celiac_7003


title: P386N_S36S43

city: Madrid

first: Carlos

last: Rodriguez Antolin

address: Paseo de la Castellana

department: Genetics

country: Spain

institute: Hospital La Paz Institute for Health Research

description: rsem-all.genes.results.txt

data_processing:
TRIMMOMATIC (v.036) was used to eliminate the remnants from the adaptors and discard those low quality sequences
Reads were mapped and quantified on the transcriptome using RSEM (version 1.3.0)
Genome_build: GRCh37
Supplementary_files_format_and_content: Tab-delimited text files include RSEM expected counts for each sample and gene

source_name: Lung Normal Sample

extract_protocol:
DNA (500 ng) from the resistant and the sensitive (used as reference) subtypes of each cell line were double-digested with RsaI and AluI for 2h at 37ºC
Poly(A)+ mRNA fraction was isolated from total RNA and cDNA libraries were obtained following Illumina ́s recommendations. Poly(A)+ RNA was isolated on poly-T oligo-attached magnetic beads and chemically fragmented prior to reverse transcription and cDNA generation. The cDNA fragments then go through an end repair process, the addition of a single ‘A’ base to the 3’ end and then ligation of the adapters. Finally, the products are purified and enriched with PCR to create the indexed final double stranded cDNA library.

value: Lung

tag: dkk1 level

tag: condition


title: P416N_S38S45

description: rsem-all.genes.results.txt


title: P394T_S37S44

description: rsem-all.genes.results.txt

source_name: Lung Tumor Sample

value: upregulated

value: Tumor


title: P325T_S33S40

description: rsem-all.genes.results.txt


title: P323N_S32S39

description: rsem-all.genes.results.txt


title: P316T_S31S38

description: rsem-all.genes.results.txt

value: downregulated


title: P378T_S34S41

description: rsem-all.genes.results.txt


title: P313T_S30S37

description: rsem-all.genes.results.txt


title: P298N_S28S35

description: rsem-all.genes.results.txt


title: P296T_S27S34

description: rsem-all.genes.results.txt


title: P300T_S29S36

description: rsem-all.genes.results.txt


title: P286T_S25S32

description: rsem-all.genes.results.txt


title: celiac_7008


title: RNA-seq_EProg_C

city: Padova

first: Oriana

last: Romano

address: Via U. Bassi 58/B

department: Molecular Medicine

country: Italy

institute: University of Padova

data_processing:
Reads quality was checked using FastQC. Raw reads were mapped to the human reference genome (hg19 build) using STAR.
Raw gene counts were obtained using HTSeq, with stranded option and Ensembl gene annotation (GRCh37 Release 82).
Counts per million (CPM) and FPKM (Fragments Per Kilobase Million) values were obtained using edgeR. Only genes with a CPM ≥ 1 in at least 3 samples were retained for subsequent analyses.
Genome_build: hg19
Supplementary_files_format_and_content: FPKM expression matrix

source_name: erythroid progenitors (E-Prog)

growth_protocol: Human CD34+ cells were purified from pooled umbilical cord blood samples obtained from several healthy donors. CD34+ hematopoietic stem/progenitor cells (HSPC) were harvested after 36 hours of culture in expansion medium. Erythroid differentiation was performed as described in Roselli et al. (Roselli et al., 2010). CD36+ erythroid progenitors (E-Prog) were selected after 5 days of culture. Differentiated erythroid precursors (E-Prec) were collected after 11 days of culture.

extract_protocol:
Total RNA was extracted from 1-2x106 HSPC, E-Prog and E-Prec (3 biological replicates for each stage) using RNeasy Micro kit (QIAGEN). RNA quality was assessed using the Agilent High Sensitivity RNA ScreenTape System (Agilent Technologies): RNA Integrity Number (RIN) was > 8 for all the samples.
Libraries were prepared starting with 1 µg of total RNA using TruSeq Stranded mRNA kit (Illumina) and 100 bp paired-end sequences were generated on the HiSeq 2500 instrument (Illumina).

value: human HSPC-derived early erythroid progenitors

value: Human umbilical cord blood


title: RNA-seq_EPrec_A

source_name: erythroid precursors (E-Prec)

value: human HSPC-derived late erythroid precursors


title: celiac_7006

value: training

value: Duodenum


title: RNA-seq_EPrec_C


title: H2Bk15ac_VCAP_R1881_Rep2

value: VCaP


title: P385N_S35S42

description: rsem-all.genes.results.txt


title: Smad7_rep3

city: Zurich

first: giancarlo

last: russo

state: Schweiz

address: winterthurerstrasse 190

department: Functional Genomics Center Zurich

country: Switzerland

institute: ETH/University of Zurich

data_processing:
bcl2Fastq v2.19 software used for demultiplexing + basecalling.
reads trimmed with trimmomatic, options: ILLUMINACLIP:adapters.fa:1:20:7:5:true AVGQUAL:10 MINLEN:20
reads mapped with STAR  v2.5.4b, options: --sjdbOverhang 150 --twopassMode None --runThreadN 8 --outFilterType BySJout --outFilterMatchNmin 30 --outFilterMismatchNmax 10 --outFilterMismatchNoverLmax 0.05 --outMultimapperOrder Random --alignSJDBoverhangMin 1 --alignSJoverhangMin 8 --alignIntronMax 1000000 --alignMatesGapMax 1000000 --outFilterMultimapNmax 50 --chimSegmentMin 15 --chimJunctionOverhangMin 15 --chimScoreMin 15 --chimScoreSeparation 10 --outSAMstrandField intronMotif --outSAMattributes All --outStd BAM_Unsorted --outSAMtype BAM Unsorted
count: FeatureCounts (Bioconductor), options: allowMultiOverlap = true; countPrimaryAlignmentsOnly= true; minFeatureOverlap=10; minMapQuality= 10 ; keepMultiHits=true
differential expression analysis: EdgeR, options: normMethod = TMM; backgroundExpression =10; testMethod = glm; deTest =QL
Genome_build: Homo Sapiens/UCSC/hg19
Supplementary_files_format_and_content: tad-delimited, raw counts

source_name: human melanoma

extract_protocol:
Total RNA were extracted by using QIAGEN RNA isolation kit and  TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
TruSeq RNA stranded

tag: genotype

value: NRAS Q61R

tag: source

value: skin


title: RNA-seq_EProg_B

tag: cell type


title: RNA-seq_HSPC_C

value: human CD34+ hematopoietic stem/progenitor cells (HSPC)


title: RNA-seq_HSPC_A

source_name: hematopoietic stem/progenitor cells (HSPC)


title: RNA-seq_EProg_A


title: RNA-seq_HSPC_B


title: Smad7_rep1


title: ctrl_rep3

value: si-Contol


title: LAPC4_R1881_DMSO_8h_rep10

data_processing:
Illumina bcl2fastq v2.17 was used to convert bcl to fastqs.
RNA-seq reads were aligned to GRCh38 using STAR aligner.
featureCounts from subread package was used to quantify reads in genes
Counts data was further analyzed in R, DESeq2 was used for differential gene expression analysis.
Genome_build: hg38
Supplementary_files_format_and_content: tab-delimited text file includes counts in genes (obtained with featureCounts) for all samples

source_name: LAPC-4 (prostate)

extract_protocol:
Cells were lyzed and RNA was isolated using RNeasy columns with on-column DNA digestion, as described by the manufacturer (Qiagen, Hilden, Germany). RNA integrity was measured and samples with values above 8 were further processed.
Libraries were prepared using Illumina TruSeq Stranded mRNA Kit and sequenced on Illumina HiSeq2500 HTv4 (single-end, dual-indexing, 50 Cycles).

treatment_protocol: Cells were stimulated with the synthetic androgen R1881 at a concentration of 1 nM after two days of starvation in medium supplemented with 10% charcoal-stripped fetal calf serum (cFCS). Darolutamide was added at a final concentration of 500 nM (low) or 2 µM (high), and the cells were harvested 8 h or 22 h post-treatment.

tag: agent 1

tag: concentration 1

tag: agent 2

value: DMSO

tag: duration


title: LAPC4_R1881_DMSO_8h_rep9

tag: cell line


title: LAPC4_R1881_DMSO_8h_rep7


title: LAPC4_R1881_DMSO_8h_rep6


title: LAPC4_R1881_DMSO_8h_rep4


title: LAPC4_R1881_DMSO_8h_rep5


title: RNA-seq_EPrec_B

tag: tissue


title: LAPC4_R1881_DMSO_8h_rep2


title: LAPC4_R1881_DMSO_8h_rep8


title: LAPC4_R1881_DMSO_8h_rep3


title: LAPC4_R1881_DMSO_22h_rep10


title: LAPC4_R1881_DMSO_22h_rep4


title: LAPC4_R1881_DMSO_22h_rep2


title: LAPC4_R1881_DMSO_22h_rep7


title: LAPC4_R1881_DMSO_22h_rep5


title: LAPC4_R1881_DMSO_22h_rep3


title: ctrl_rep2

tag: treatment


title: ctrl_rep1


title: LAPC4_R1881_DMSO_22h_rep1


title: LAPC4_R1881_DMSO_8h_rep1


title: LAPC4_R1881_Daro_2uM_8h_rep2

value: Daro

tag: concentration 2


title: LAPC4_R1881_Daro_2uM_8h_rep5


title: LAPC4_R1881_DMSO_22h_rep6


title: LAPC4_R1881_Daro_2uM_8h_rep4


title: Smad7_rep2


title: LAPC4_R1881_Daro_2uM_8h_rep3


title: LAPC4_R1881_Daro_2uM_22h_rep5


title: LAPC4_R1881_Daro_2uM_8h_rep1


title: LAPC4_R1881_DMSO_22h_rep8


title: LAPC4_R1881_DMSO_22h_rep9


title: P292T_S26S33

description: rsem-all.genes.results.txt


title: hAAC_IFNg_stimulated_donor5

type: RNA

first: Karsten

last: Jürchott

address: Luisenstraße

department: Rahel Hirsch Center

institute: BIH at Charité

hyb_protocol: 12.5 µg fragmented aRNA was used for hybridisation on the chip for 16 h at 45 °C.

scan_protocol: Finally, the chips were washed, stained and scanned using the Affymetrix Gene Chip Scanner 3000. Affymetrix GeneChip Operating Software (GCOS) 1.4 was used to generate CEL data files.

data_processing: CEL files were further processed in R using the package affy. Data were normalized using quantile normalization and intensities were summarized and log2-transformed using the rma algorithm implemented in the affy-package.

label: biotin

source_name: atrial appendage-derived cells

label_protocol: RNA samples of unstimulated and IFNγ pre-stimulated hAACs were prepared with GeneChip® 3′ IVT Express Kit and GeneChip® Hybridisation, Wash and Stain Kit (Affymetrix) according to the manufacturer’s instructions. In brief, 250 ng total RNA was used for cDNA synthesis and subsequent in vitro transcription (IVT) to amplified RNA (aRNA).

growth_protocol: The CD90low human atrial appendage-derived cell (hAAC) population (from three different donors respectively) was grown under standard culture conditions (37°C in a 21% O2 and 5% CO2 atmosphere) at a density of 6000 cells/cm² in complete medium (cIDH) consisting of equal amounts of IMDM/DMEM/Ham’s F12 (IDH; all Biochrom, Berlin, Germany) and supplemented with 5% male heat-inactivated human AB serum (Sigma-Aldrich, St. Louis, MO, USA), 100 U/mL penicillin and 100 µg/mL streptomycin (Gibco® Life Technologies, Grand Island, NY, USA), 20ng/mL basic fibroblast growth factor and 10ng/mL epithelial growth factor (both from Preprotech, Hamburg, Germany) for expansion and subsequent harvest with 0.05% trypsin/0.02% EDTA (Biochrom).

extract_protocol: Cells were harvested and lysed with TriReagent (Sigma) according to the protocol of the manufacturer. 133 µl Brom-Chlor-Propan was added to 1ml cell suspension and vortexed for 15 minutes at 1350 rpm. After centrifugation (60 minutes with 17000g/13300rpm) the supernatants were removed and applied to  Rneasy Mini columns for further purification.

treatment_protocol: Three different hAACs donors were reseeded into T25 cell culture flasks (Falcon, BD Biosciences, San Jose, CA, USA) at a density of 6000 cells/cm² in cIDH medium. After attachment over-night medium was exchanged with cIDH that was either supplemented with 100 ng/mL of interferon-gamma (IFNγ; Miltenyi Biotec, Bergisch Gladbach, Germany) or left unstimulated and cells were incubated for 48 hours at standard culture conditions.

tag: donor

value: IFNγ-stimulated

value: atrial appendage-derived cells


title: hAAC_non_treated_donor1

value: non-treated


title: hAAC_IFNg_stimulated_donor1


title: hAAC_non_treated_donor4


title: hAAC_IFNg_stimulated_donor4


title: eL3_EAD_NR_ey>Delta,COX7aRNAi_rep1

city: London

first: Sebastian

last: Sorge

address: 1 Midland Road

institute: The Francis Crick Institute

hyb_protocol: Samples were hybridized for 16 hours at 45°C (at 60rpm) on Drosophila Genome 2.0 Arrays. Arrays were washed and stained in the Affymetrix Fluidics Station 450 (Affymetrix GeneChip® Hybridization, Wash, and Stain Kit, catalogue number 900720).

scan_protocol: Arrays were scanned with a GeneChip Scanner 3000 7G.

data_processing: CEL files were interpreted with Affymetrix Expression Console (Build 1.4.1.46) using RMA normalisation (default settings). CHP files were used for gene expression analysis with Affymetrix Transcriptome Analysis Console (Version 3.0.0.466).

organism: Drosophila melanogaster

source_name: Early L3 (150h AEL) larval eye-antenna disc.

label_protocol: 50ng total RNA was used for in vitro amplification using Affymetrix GeneChip® 3’ IVT PLUS Reagent Kit according to the manufacturers specifications.

growth_protocol: Fly crosses were set up in small cages and females allowed to lay eggs on petri dishes with NR food (protein restricted, 20g fresh yeast per litre) for 2 to 3 hours. Larvae were then grown in the dishes until they reached the appropriate early L3 stage (150h AEL).

extract_protocol: For RNA extraction, samples were thawed and centrifuged at 12000rpm for 20' at 4°C. 1ml supernatant was transferred to a new tube containing 1ml 100% Ethanol. The mixture was then run stepwise through a ZymoResearch Direct-zol MicroPrep column, following the manufacturers protocol, including on-column DNAseI digest. Total RNA was eluted in 8µl pure water.

treatment_protocol: Larvae were dissected under a dissecting microscope, eye-antenna discs manually isolated and transferred to a tube with PBS and 0.001% Tween-20 on ice. When 20 to 30 eye-antenna discs (depending on size) were collected, supernatant was carefully removed and presence of discs in tube confirmed under the microscope. 1ml TRIzol was added and continously vortexed to allow fast and efficient lysis. Samples were then frozen at -80°C.


title: eL3_EAD_ey>Delta_rep2

source_name: Early L3 (96h AEL) larval eye-antenna disc.

label_protocol: 50ng total RNA was used for in vitro amplification using Affymetrix GeneChip® 3’ IVT Express Reagent Kit according to the manufacturers specifications.

growth_protocol: Fly crosses were set up in small cages and females allowed to lay eggs on petri dishes with standard (cornmeal, molasses, yeast extract) fly food for 2 to 3 hours. Larvae were then grown in the dishes until they reached the appropriate early L3 stage (96h AEL).

value: ey-Gal4, UAS-Delta/UAS-GFP


title: eL3_EAD_NR_ey>COX7aRNAi_rep2

value: ey-Gal4/UAS-COX7a-RNAi (v106661)


title: eL3_EAD_ey>Notch[intra2]_rep1


title: eL3_EAD_ey>Delta_rep1


title: eL3_EAD_ey>Delta,ATF4RNAi_rep1


title: eL3_EAD_NR_ey>GFP_rep1


title: eL3_EAD_ey>COX7aRNAi_rep2


title: eL3_EAD_NR_ey>ATF4RNAi_rep2


title: eL3_EAD_NR_ey>COX7aRNAi,ATF4RNAi_rep1


title: eL3_EAD_ey>Delta,COX7aRNAi_rep1


title: eL3_EAD_NR_ey>COX7aRNAi,ATF4RNAi_rep2


title: eL3_EAD_NR_ey>Delta,COX7aRNAi,ATF4RNAi_rep2

value: ey-Gal4, UAS-Delta/UAS-COX7a-RNAi (v106661);ATF4-RNAi (BL-25985)/+


title: eL3_EAD_ey>Notch[intra2]_rep2


title: eL3_EAD_NR_ey>Delta,COX7aRNAi_rep2


title: eL3_EAD_NR_ey>Delta,ATF4RNAi_rep2


title: eL3_EAD_NR_ey>Delta,GFP_rep2


title: eL3_EAD_NR_ey>COX7aRNAi_rep1


title: eL3_EAD_NR_ey>GFP_rep2


title: eL3_EAD_NR_ey>Delta,GFP_rep1


title: eL3_EAD_NR_ey>Delta,COX7aRNAi,ATF4RNAi_rep1


title: eL3_EAD_NR_ey>ATF4RNAi_rep1


title: eL3_EAD_ey>COX7aRNAi_rep1


title: eL3_EAD_ey>Delta,COX7aRNAi_rep2


title: eL3_EAD_ey>GFP_rep2


title: eL3_EAD_ey>GFP_rep1


title: D18,  biological rep3

city: Dubna

first: Mikhail

last: Zarubin

address: Russia, Moscow region, Dubna, Juliot Curie, 20

country: Russia

institute: Joint Institute for Nuclear Research

description: Gene expression data from  3-day-old adult D.melanogaster

hyb_protocol: Hybridisation was performed with GeneChip™ Hybridization, Wash and Stain Kit according to the manufacturers' instructions. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.

scan_protocol: GeneChips were scanned using the GeneChip™ Scanner 3000 7G

data_processing: The data were analyzed using the Transcriptom analysis console software version 4.0.1.

source_name: Drosophila adult fly

label_protocol: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250 ng total RNA (according GeneChip™ 3' IVT PLUS Reagent Kit Protocol)

growth_protocol: The strains were cultured on a standard cornmeal-molasses medium with antibiotic and maintained at 25 °C

tag: strain


title: D18,  biological rep2

extract_protocol: The strains were cultured on a standard cornmeal-molasses medium with antibiotic and maintained at 25 °C


title: D18,  biological rep1


title: BAF3 RET rep3

city: Shanghai

first: Min

last: Huang

address: 555, Zuchongzhi Road

country: China

institute: Shanghai Institute of Materia Medica

data_processing:
Index of the reference genome was built using STAR and paired-end clean reads were aligned to the reference genome using STAR (v2.5.1b)
HTSeq v0.6.0 was used to count the reads numbers mapped to each gene. And then FPKM of each gene was calculated based on the length of the gene and reads count mapped to this gene
Genome_build: GRCm38
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample ...

organism: Mus musculus

source_name: BAF3 RET rep3

growth_protocol: BAF3 cells were maintained in RPMI-1640 supplemented with 10% FBS.

extract_protocol:
Total RNA was extracted with TRIzol reagent following the recommendations of the manufacturer.
RNA libraries were prepared for sequencing using standard Illumina protocols

value: Pro-B cells

value: CCDC6-RET fusion


title: BAF3 RET rep1

source_name: BAF3 RET rep1


title: BAF3 FGFR rep3

source_name: BAF3 FGFR rep3

value: TEL-FGFR1 fusion


title: BAF3 MET rep2

source_name: BAF3 MET rep2

value: TPR-MET fusion


title: BAF3 MET rep1

source_name: BAF3 MET rep1


title: BAF3 FGFR rep1

source_name: BAF3 FGFR rep1


title: BAF3 EGFR rep3

source_name: BAF3 EGFR rep3

value: EGFR L858R T790M


title: BAF3 WT rep1

source_name: BAF3 WT rep1

value: wild type


title: BAF3 EGFR rep2

source_name: BAF3 EGFR rep2


title: BAF3 WT rep3

source_name: BAF3 WT rep3


title: BAF3 EGFR rep1

source_name: BAF3 EGFR rep1


title: BAF3 MET rep3

source_name: BAF3 MET rep3


title: BAF3 WT rep2

source_name: BAF3 WT rep2


title: Canton-S,  biological rep2

value: Canton-S


title: Canton-S,  biological rep1


title: BAF3 RET rep2

source_name: BAF3 RET rep2


title: BAF3 FGFR rep2

source_name: BAF3 FGFR rep2


title: Canton-S,  biological rep3


title: Blood, Non-smoker_457

type: genomic

city: Hangzhou

first: Yi

last: Xu

state: Zhejiang

address: No. 17, Dazhi Road, old Zhejiang, Shangcheng District

department: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases

institute: The First Affiliated Hospital, Zhejiang University School of Medicine

hyb_protocol: RA1 resuspended DNA was loaded onto BeadChips arrays. After overnight incubation at 48 °C, single-base extension and allele-specific staining was performed on a Teflow chamber rack system (Tecan, Maennedorf, Switzerland).

scan_protocol: All samples were genotyped using the Infinium Human Exome-12 v1.1 BeadChip (Illumina, Inc., San Diego, CA, USA) according to the manufacturer’s protocol.

data_processing:
After genotyping, we performed the following quality control analyses on all genotyped SNPs. First, among 242 901 genotyped variants, we excluded all insertions and deletions to ensure that all base pair positions were unique and referred to the same variant. Second, any SNP with a call rate of <95% was excluded. Third, we removed those SNPs that were not in Hardy–Weinberg equilibrium at a p value of < 1.0 × 10−6. Finally, we used a 5% minor allele frequency (MAF) as a threshold to define rare and common variants, and any SNP with an MAF of <0.05 was excluded from the analysis.
[value definition]  Genotype: AA,AB,BB,or NC (no call)

label: cy3, cy5

source_name: Blood

label_protocol: The concentration of each DNA sample was first measured using the QuantiT™ dsDNA assay kit (Life Technologies, Carlsbad, CA) and then 50 DNA samples were pooled in equimolar amounts, as suggested by the manufacturers.

extract_protocol: Genomic DNA was extracted from EDTA-treated peripheral venous blood of each subject using the Qiagen DNA purification kit. All DNA samples were treated with RNase A to remove potential contaminating RNA, and DNA quality and the concentration of each sample was determined by the A260/A280 absorbance ratio.

tag: ethnicity

value: African-American

tag: age

tag: gender

value: Female

tag: cpd

tag: hsi

tag: ftnd

value: Non-smoker

value: Blood


title: Blood, Non-smoker_455


title: Blood, Non-smoker_452


title: Blood, Smoker_454

value: Smoker


title: Blood, Smoker_453


title: Blood, Non-smoker_460


title: Blood, Non-smoker_459


title: Blood, Non-smoker_451


title: Blood, Non-smoker_450


title: Blood, Non-smoker_458

value: Male


title: Blood, Non-smoker_445


title: Blood, Non-smoker_442


title: Blood, Non-smoker_447


title: Pressure-treated-1 mRNA

first: Shen

last: si

address: No. 500 Chengdu North Road

institute: Shanghai Changzheng hospital

description: human hepatocellular carcinoma cell line HepG2, pressurized, 15mmHg, 24h

hyb_protocol: The labeled/amplified RNAs and labeled cRNAs QC were purified by RNeasy Mini Kit (Qiagen p/n 74104). Each slide was hybridized with cyanine 3-labeled RNA using Agilent Gene Expression Hybridization Kit (Agilent p/n 5188-5242) in hybridization Oven (Agilent p/n G2545A) at 65°C for 17 hours according to the manufacturer’s instructions, hybridization section.

scan_protocol: After hybridization, slides were washed in staining dishes (Thermo Shandon p/n 121) with Gene Expression Wash Buffer 1 (Agilent p/n 5188-5325) and Gene Expression Wash Buffer 2 (Agilent p/n 5188-5326). Slides were scanned by Agilent Microarray Scanner (Agilent p/n G2505C) and Feature Extraction software Agilent Feature Extraction with default settings.

data_processing: The scanned images were analyzed with Feature Extraction Software 10.7.1.1 (Agilent) .And then using Software Genespring(version13.1, Agilent Technologies) to analysis.

label_protocol: Quick Amp Labeling Kit, One-Color (Agilent p/n 5190-2305) was used to prepare label reaction followed the manufacturer’s instructions, labeling section.

growth_protocol: For 3 dimensional (3D) culture the cells were resuspended in 1XAccutase enzymes (Innovative Cell Technologies, Santiago, American), and the concentration of the cell suspension was adjusted to 6×106cells/ml.Then 37.6ul cell suspension,22.8ul water, 6.2ul 10×CBpH5.5 and 13.4ul Mal-Dextran were gently mixed and the mixture was fully mixed with 20ul PEG-link. After 3 times of speed blow, the mixture was rested for 5min.After the hydrogel solidified, a proper amount of DMEM containing 10% FBS was added and cultured for 4h.Then replace the culture medium for new before the pressure test began.

extract_protocol: Total RNA was quantified by NanoDrop ND-2000 (Thermo Science) and detected by Agilent Bioanalyzer 2100 (Agilent Technology).

treatment_protocol: Pressure loading of 3D cultured cells was performed (30mmHg, 24h) using the compression system Flexcell 5000C (Flexcell International,McKeesport,PA;see their Web site at www.flexcelllint.com for a description). Three dimensional cell gel was placed in Flexcell BioPress compression culture 6-well plates,and set pressure parameters on the computer,including compressive strength(1.995kpa/15mmHg) and intervention time(24h). The cells in gel were detached by 1XAccutase enzymes, and centrifuged 10min at 1000rpm. Then the cells were collected for follow-up experiments.

tag: cell type

value: human hepatocellular carcinoma


title: Blood, Smoker_448


title: Blood, Smoker_443


title: Blood, Non-smoker_446


title: Blood, Non-smoker_449


title: Blood, Smoker_441


title: Pressure-treated-2 mRNA

source_name: human hepatocellular carcinoma cell line HepG2, pressurized, 15mmHg, 24h


title: Control-2 mRNA

description: Gene expression after cultured for 24h

source_name: human hepatocellular carcinoma cell line HepG2


title: Control-1 mRNA

description: Gene expression after cultured for 24h


title: Blood, Non-smoker_439


title: Blood, Non-smoker_440


title: Blood, Non-smoker_456


title: Blood, Non-smoker_437


title: Blood, Smoker_438


title: Blood, Non-smoker_436


title: Blood, Non-smoker_444


title: Blood, Non-smoker_433


title: Blood, Smoker_432


title: Blood, Non-smoker_430


title: Blood, Smoker_431


title: Blood, Smoker_435


title: Blood, Non-smoker_429


title: Blood, Non-smoker_428


title: Blood, Non-smoker_418


title: Blood, Smoker_426


title: Blood, Non-smoker_421


title: Blood, Smoker_423


title: Blood, Non-smoker_425


title: Blood, Smoker_424


title: Blood, Non-smoker_427


title: Blood, Non-smoker_422


title: Blood, Non-smoker_419


title: Blood, Non-smoker_420


title: Blood, Smoker_415


title: Blood, Non-smoker_411


title: Blood, Non-smoker_417


title: Blood, Non-smoker_414


title: Blood, Smoker_416


title: Blood, Non-smoker_412


title: Blood, Non-smoker_434


title: Blood, Non-smoker_409


title: Blood, Non-smoker_410


title: Blood, Non-smoker_406


title: Blood, Smoker_408


title: Blood, Non-smoker_407


title: Blood, Non-smoker_404


title: Blood, Non-smoker_405


title: Blood, Non-smoker_403


title: Blood, Non-smoker_401


title: Blood, Non-smoker_402


title: Blood, Smoker_413


title: Blood, Non-smoker_399


title: Blood, Non-smoker_396


title: Blood, Non-smoker_397


title: Blood, Non-smoker_392


title: Blood, Non-smoker_400


title: Blood, Non-smoker_387


title: Blood, Non-smoker_394


title: Blood, Non-smoker_393


title: Blood, Smoker_398


title: Blood, Non-smoker_385


title: Blood, Non-smoker_389


title: Blood, Non-smoker_383


title: Blood, Non-smoker_381


title: Blood, Non-smoker_388


title: Blood, Smoker_384


title: Blood, Smoker_386


title: Blood, Smoker_382


title: Blood, Non-smoker_380


title: Blood, Non-smoker_390


title: Blood, Smoker_378


title: Blood, Smoker_374


title: Blood, Non-smoker_373


title: Blood, Smoker_376


title: Blood, Smoker_379


title: Blood, Non-smoker_375


title: Blood, Non-smoker_368


title: Blood, Smoker_364


title: Blood, Non-smoker_367


title: Blood, Non-smoker_377


title: Blood, Smoker_395


title: Blood, Non-smoker_391


title: Blood, Non-smoker_369


title: Blood, Non-smoker_355


title: Blood, Smoker_358


title: Blood, Non-smoker_357


title: Blood, Non-smoker_365


title: Blood, Non-smoker_360


title: Blood, Non-smoker_372


title: Blood, Non-smoker_361


title: Blood, Non-smoker_366


title: Blood, Smoker_362


title: Blood, Non-smoker_363


title: Blood, Non-smoker_356


title: Blood, Non-smoker_359


title: Blood, Non-smoker_353


title: Blood, Non-smoker_352


title: Blood, Non-smoker_351


title: Blood, Non-smoker_350


title: Blood, Non-smoker_370


title: Blood, Non-smoker_354


title: Blood, Non-smoker_371


title: Blood, Smoker_348


title: Blood, Non-smoker_347


title: Blood, Smoker_349


title: Blood, Non-smoker_346


title: Blood, Smoker_344


title: Blood, Non-smoker_345


title: Blood, Non-smoker_341


title: Blood, Non-smoker_342


title: Blood, Non-smoker_336


title: Blood, Smoker_333


title: Blood, Non-smoker_339


title: Blood, Non-smoker_334


title: Blood, Non-smoker_335


title: Blood, Smoker_330


title: Blood, Non-smoker_332


title: Blood, Non-smoker_331


title: Blood, Non-smoker_338


title: Blood, Non-smoker_328


title: Blood, Smoker_327


title: Blood, Non-smoker_326


title: Blood, Non-smoker_343


title: Blood, Smoker_325


title: Blood, Non-smoker_324


title: Blood, Smoker_323


title: Blood, Non-smoker_322


title: Blood, Non-smoker_321


title: Blood, Smoker_320


title: Blood, Non-smoker_318


title: Blood, Non-smoker_319


title: Blood, Non-smoker_340


title: Blood, Smoker_317


title: Blood, Smoker_329


title: Blood, Non-smoker_314


title: Blood, Smoker_315


title: Blood, Non-smoker_316


title: Blood, Smoker_312


title: Blood, Non-smoker_309


title: Blood, Smoker_310


title: Blood, Non-smoker_305


title: hAAC_non_treated_donor5

source_name: atrial appendage-derived cells

value: non-treated

value: atrial appendage-derived cells


title: Blood, Non-smoker_337


title: Blood, Non-smoker_308


title: Blood, Non-smoker_303


title: Blood, Smoker_307


title: Blood, Smoker_300


title: Blood, Smoker_301


title: Blood, Non-smoker_299


title: Blood, Smoker_302


title: Blood, Non-smoker_294


title: Blood, Smoker_295


title: Blood, Non-smoker_296


title: Blood, Non-smoker_292


title: Blood, Smoker_297


title: Blood, Smoker_291


title: Blood, Smoker_293


title: Blood, Non-smoker_290


title: Blood, Non-smoker_306


title: Blood, Non-smoker_289


title: Blood, Smoker_288


title: Blood, Non-smoker_298


title: Blood, Non-smoker_287


title: Blood, Smoker_286


title: Blood, Non-smoker_281


title: Blood, Smoker_282


title: Blood, Smoker_283


title: Blood, Smoker_284


title: Blood, Non-smoker_278


title: Blood, Non-smoker_277


title: Blood, Non-smoker_279


title: Blood, Non-smoker_280


title: Blood, Non-smoker_275


title: Blood, Smoker_276


title: Blood, Non-smoker_273


title: Blood, Smoker_274


title: Blood, Smoker_272


title: Blood, Smoker_268


title: Blood, Smoker_271


title: Blood, Non-smoker_269


title: Blood, Non-smoker_267


title: Blood, Smoker_264


title: Blood, Non-smoker_285


title: Blood, Non-smoker_266


title: Blood, Non-smoker_262


title: Blood, Non-smoker_261


title: Blood, Non-smoker_260


title: Blood, Non-smoker_263


title: Blood, Smoker_259


title: Blood, Smoker_304


title: Blood, Non-smoker_256


title: Blood, Non-smoker_255


title: Blood, Non-smoker_258


title: Blood, Non-smoker_254


title: Blood, Non-smoker_252


title: Blood, Non-smoker_249


title: Blood, Non-smoker_257


title: Blood, Non-smoker_253


title: Blood, Non-smoker_251


title: Blood, Smoker_270


title: Blood, Non-smoker_247


title: Blood, Non-smoker_265


title: Blood, Non-smoker_246


title: Blood, Non-smoker_250


title: Blood, Smoker_244


title: Blood, Non-smoker_242


title: Blood, Smoker_241


title: Blood, Smoker_240


title: Blood, Smoker_238


title: Blood, Smoker_237


title: Blood, Smoker_235


title: Blood, Non-smoker_248


title: Blood, Smoker_233


title: Blood, Smoker_231


title: Blood, Smoker_230


title: Blood, Smoker_228


title: Blood, Smoker_232


title: Blood, Smoker_229


title: Blood, Non-smoker_245


title: Blood, Smoker_224


title: Blood, Smoker_225


title: Blood, Smoker_243


title: Blood, Smoker_222


title: Blood, Smoker_227


title: Blood, Smoker_226


title: Blood, Smoker_223


title: Blood, Smoker_218


title: Blood, Smoker_219


title: Blood, Smoker_239


title: Blood, Smoker_221


title: Blood, Smoker_217


title: Blood, Smoker_236


title: Blood, Smoker_216


title: Blood, Smoker_311


title: Blood, Smoker_234


title: Blood, Smoker_215


title: Blood, Smoker_214


title: Blood, Smoker_213


title: Blood, Smoker_212


title: Blood, Smoker_211


title: Blood, Smoker_210


title: Blood, Smoker_209


title: Blood, Smoker_206


title: Blood, Smoker_203


title: Blood, Smoker_205


title: Blood, Smoker_204


title: Blood, Smoker_201


title: Blood, Smoker_202


title: Blood, Smoker_207


title: Blood, Smoker_199


title: Blood, Smoker_197


title: Blood, Smoker_208


title: Blood, Smoker_195


title: Blood, Smoker_194


title: Blood, Smoker_193


title: Blood, Smoker_196


title: Blood, Smoker_192


title: Blood, Smoker_191


title: Blood, Smoker_189


title: Blood, Smoker_190


title: Blood, Smoker_187


title: Blood, Smoker_188


title: Blood, Smoker_185


title: Blood, Smoker_186


title: Blood, Non-smoker_184


title: Blood, Non-smoker_183


title: Blood, Non-smoker_182


title: Blood, Non-smoker_181


title: Blood, Smoker_200


title: Blood, Smoker_198


title: Blood, Non-smoker_179


title: Blood, Smoker_175


title: Blood, Smoker_173


title: Blood, Smoker_174


title: Blood, Smoker_176


title: Blood, Smoker_177


title: Blood, Smoker_172


title: Blood, Smoker_168


title: Blood, Smoker_171


title: Blood, Non-smoker_313


title: Blood, Smoker_166


title: Blood, Smoker_169


title: Blood, Smoker_167


title: Blood, Smoker_170


title: Blood, Smoker_165


title: Blood, Smoker_159


title: Blood, Smoker_162


title: Blood, Smoker_164


title: Blood, Smoker_160


title: Blood, Smoker_161


title: Blood, Smoker_158


title: Blood, Smoker_163


title: Blood, Smoker_155


title: Blood, Smoker_157


title: Blood, Smoker_153


title: Blood, Non-smoker_180


title: Blood, Smoker_156


title: Blood, Smoker_152


title: Blood, Smoker_154


title: Blood, Smoker_178


title: Blood, Smoker_149


title: Blood, Smoker_148


title: Blood, Smoker_150


title: Blood, Smoker_145


title: Blood, Smoker_146


title: Blood, Non-smoker_147


title: Blood, Smoker_134


title: Blood, Non-smoker_132


title: Blood, Non-smoker_144


title: Blood, Smoker_143


title: Blood, Smoker_142


title: Blood, Non-smoker_138


title: Blood, Smoker_136


title: Blood, Non-smoker_133


title: Blood, Smoker_140


title: Blood, Non-smoker_141


title: Blood, Smoker_137


title: Blood, Smoker_129


title: Blood, Smoker_130


title: Blood, Smoker_131


title: Blood, Smoker_151


title: Blood, Smoker_125


title: Blood, Smoker_127


title: Blood, Smoker_126


title: Blood, Non-smoker_128


title: Blood, Smoker_124


title: Blood, Smoker_135


title: Blood, Non-smoker_139


title: Blood, Smoker_123


title: Blood, Smoker_220


title: Blood, Smoker_121


title: Blood, Smoker_117


title: Blood, Non-smoker_119


title: Blood, Smoker_120


title: Blood, Smoker_118


title: Blood, Smoker_112


title: Blood, Non-smoker_114


title: Blood, Smoker_113


title: Blood, Smoker_110


title: Blood, Smoker_115


title: Blood, Smoker_106


title: Blood, Smoker_107


title: Blood, Non-smoker_122


title: Blood, Non-smoker_111


title: Blood, Smoker_108


title: Blood, Non-smoker_116


title: Blood, Smoker_105


title: Blood, Smoker_109


title: Blood, Smoker_102


title: Blood, Smoker_103


title: Blood, Smoker_101


title: Blood, Smoker_100


title: Blood, Smoker_98


title: Blood, Smoker_96


title: Blood, Smoker_97


title: Blood, Smoker_93


title: Blood, Smoker_95


title: Blood, Non-smoker_94


title: Blood, Smoker_90


title: Blood, Smoker_91


title: Blood, Non-smoker_89


title: Blood, Smoker_92


title: Blood, Smoker_88


title: Blood, Ex-smoker_85

value: Ex-smoker


title: Blood, Smoker_86


title: Blood, Smoker_104


title: Blood, Smoker_99


title: Blood, Smoker_84


title: Blood, Smoker_87


title: Blood, Smoker_76


title: Blood, Smoker_83


title: Blood, Smoker_79


title: Blood, Smoker_80


title: Blood, Smoker_81


title: Blood, Smoker_78


title: Blood, Smoker_77


title: Blood, Non-smoker_74


title: Blood, Non-smoker_75


title: Blood, Smoker_72


title: Blood, Smoker_70


title: Blood, Non-smoker_71


title: Blood, Smoker_69


title: Blood, Smoker_65


title: Blood, Smoker_67


title: Blood, Smoker_68


title: Blood, Smoker_73


title: Blood, Smoker_66


title: Blood, Smoker_82


title: Blood, Smoker_64


title: Blood, Smoker_63


title: Blood, Ex-smoker_61


title: Blood, Ex-smoker_57


title: Blood, Smoker_58


title: Blood, Smoker_60


title: Blood, Smoker_59


title: Blood, Smoker_56


title: Blood, Smoker_55


title: Blood, Smoker_53


title: Blood, Smoker_51


title: Blood, Smoker_54


title: Blood, Smoker_49


title: Blood, Smoker_50


title: Blood, Smoker_52


title: Blood, Smoker_47


title: Blood, Smoker_44


title: Blood, Smoker_46


title: Blood, Smoker_45


title: Blood, Non-smoker_43


title: Blood, Smoker_42


title: Blood, Smoker_37


title: Blood, Smoker_41


title: Blood, Smoker_40


title: Blood, Smoker_38


title: Blood, Smoker_36


title: Blood, Smoker_62


title: Blood, Smoker_33


title: Blood, Smoker_35


title: Blood, Smoker_32


title: Blood, Smoker_34


title: Blood, Smoker_39


title: Blood, Smoker_48


title: Blood, Smoker_31


title: Blood, Smoker_29


title: Blood, Smoker_28


title: Blood, Smoker_26


title: Blood, Smoker_30


title: Blood, Smoker_25


title: Blood, Smoker_27


title: Blood, Smoker_21


title: Blood, Smoker_22


title: Blood, Smoker_19


title: Blood, Smoker_24


title: Blood, Smoker_15


title: Blood, Non-smoker_16


title: Blood, Smoker_11


title: Blood, Smoker_12


title: Blood, Smoker_17


title: Blood, Smoker_13


title: Blood, Smoker_14


title: Blood, Smoker_10


title: Blood, Non-smoker_8


title: Blood, Smoker_5


title: Blood, Smoker_7


title: Blood, Smoker_9


title: Blood, Smoker_6


title: Blood, Smoker_4


title: Blood, Smoker_3


title: Blood, Smoker_2


title: Blood, Smoker_23


title: Blood, Smoker_1


title: Blood, Smoker_20


title: Blood, Non-smoker_18


title: CR6

city: BUSAN

first: Kyungbin

last: Kim

address: 179, Gudeok-ro

country: South Korea

institute: Pusan National University Hospital

hyb_protocol: Hybridizations were carried out by combining 5µl of each RNA sample with 8µl nCounter Reporter probes in hybridization buffer and 2µl nCounter Capture probes (total reaction volume of 15µl), and allowed to incubate overnight at 65°C for 16–30 hours. Excess probes were removed using a two-step magnetic bead-based purification on the nCounter Prep Station (nanoString Technologies).

scan_protocol: Amount of specific target molecules were quantified on the nCounter Digital Analyzer by counting the individual fluorescent barcodes and assessing the target molecules. For each assay, a high-density scan encompassing 280 fields of view was performed. The data was collected using the nCounter Digital Analyzer, after taking images of the immobilized fluorescent reporters in the sample cartridge with a CCD camera.

data_processing: mRNA data analysis, including measurement of fold change, was performed using the nSolver™ software analysis (freely available from nanoString Technologies). The mRNA profiling data was normalized using housekeeping genes.

source_name: epithelial ovarian cancer

growth_protocol: fresh EOC tissue stored at the Nationa Biobank

extract_protocol: Total RNA was extracted using RNeasy mini prep kit (Qiagen, Valencia, CA, USA). The RNA yield and purity were assessed using a DS 11 Spectrophotometer (Denovix Inc, DE, USA).

tag: phenotype

value: chemoresistant

tag: histology

value: high grade serous carcinoma


title: CR5


title: CR2


title: CS5

value: chemosensitive


title: CR4


title: CR1


title: CS1


title: Blood, Non-smoker_3184


title: Blood, Non-smoker_3181


title: Blood, Smoker_3182


title: Blood, Smoker_3186


title: CS2


title: Blood, Smoker_3180


title: Blood, Smoker_3187


title: Blood, Non-smoker_3179


title: CS6


title: Blood, Non-smoker_3178


title: Blood, Smoker_3177


title: Blood, Smoker_3188


title: CS3


title: Blood, Non-smoker_3183


title: Blood, Smoker_3175


title: Blood, Non-smoker_3174


title: Blood, Smoker_3172


title: Blood, Non-smoker_3173


title: CR3


title: Blood, Non-smoker_3171


title: Blood, Non-smoker_3185


title: CS4


title: Blood, Smoker_3170


title: Blood, Smoker_3176


title: Blood, Smoker_3169


title: Blood, Smoker_3168


title: Blood, Smoker_3167


title: Blood, Smoker_3160


title: Blood, Smoker_3155


title: Blood, Smoker_3158


title: Blood, Smoker_3163


title: Blood, Smoker_3165


title: Blood, Smoker_3161


title: Blood, Smoker_3159


title: Blood, Smoker_3164


title: Blood, Smoker_3157


title: Blood, Smoker_3152


title: Blood, Smoker_3162


title: Blood, Smoker_3150


title: Blood, Smoker_3147


title: Blood, Non-smoker_3148


title: Blood, Smoker_3151


title: Blood, Non-smoker_3146


title: Blood, Non-smoker_3144


title: Blood, Non-smoker_3149


title: Blood, Non-smoker_3145


title: Blood, Smoker_3156


title: Blood, Smoker_3154


title: Blood, Non-smoker_3143


title: Blood, Non-smoker_3142


title: Blood, Non-smoker_3139


title: Blood, Non-smoker_3140


title: Blood, Smoker_3166


title: Blood, Smoker_3153


title: Blood, Non-smoker_3137


title: Blood, Smoker_3136


title: Blood, Non-smoker_3133


title: Blood, Non-smoker_3135


title: Blood, Smoker_3129


title: Blood, Non-smoker_3131


title: Blood, Non-smoker_3132


title: Blood, Non-smoker_3127


title: Blood, Non-smoker_3130


title: Blood, Non-smoker_3128


title: Blood, Non-smoker_3124


title: Blood, Smoker_3123


title: Blood, Non-smoker_3119


title: Blood, Non-smoker_3122


title: Blood, Smoker_3120


title: Blood, Smoker_3121


title: Blood, Smoker_3117


title: Blood, Smoker_3116


title: Blood, Smoker_3141


title: Blood, Non-smoker_3125


title: Blood, Smoker_3112


title: Blood, Non-smoker_3118


title: Blood, Smoker_3138


title: Blood, Smoker_3115


title: Blood, Non-smoker_3114


title: Blood, Smoker_3110


title: Blood, Non-smoker_3111


title: Blood, Non-smoker_3134


title: Blood, Non-smoker_3126


title: Blood, Non-smoker_3107


title: Blood, Non-smoker_3106


title: Blood, Smoker_3099


title: Blood, Non-smoker_3103


title: Blood, Smoker_3100


title: Blood, Non-smoker_3102


title: Blood, Smoker_3104


title: Blood, Non-smoker_3108


title: Blood, Smoker_3101


title: Blood, Non-smoker_3113


title: Blood, Smoker_3098


title: Blood, Smoker_3097


title: Blood, Non-smoker_3091


title: Blood, Non-smoker_3093


title: Blood, Non-smoker_3095


title: Blood, Smoker_3094


title: Blood, Smoker_3090


title: Blood, Non-smoker_3092


title: Blood, Smoker_3088


title: Blood, Non-smoker_3087


title: Blood, Non-smoker_3085


title: Blood, Non-smoker_3086


title: Blood, Non-smoker_3084


title: Blood, Non-smoker_3109


title: Blood, Non-smoker_3083


title: Blood, Smoker_3082


title: Blood, Non-smoker_3105


title: Blood, Smoker_3081


title: Blood, Non-smoker_3080


title: Blood, Non-smoker_3078


title: Blood, Non-smoker_3096


title: Blood, Non-smoker_3077


title: Blood, Non-smoker_3079


title: Blood, Non-smoker_3075


title: Blood, Smoker_3074


title: Blood, Smoker_3070


title: Blood, Non-smoker_3073


title: Blood, Non-smoker_3069


title: Blood, Non-smoker_3067


title: Blood, Smoker_3071


title: Blood, Non-smoker_3065


title: Blood, Non-smoker_3062


title: Blood, Non-smoker_3060


title: Blood, Non-smoker_3063


title: Blood, Non-smoker_3066


title: Blood, Non-smoker_3059


title: Blood, Non-smoker_3057


title: Blood, Non-smoker_3061


title: Blood, Smoker_3058


title: Blood, Non-smoker_3056


title: Blood, Smoker_3054


title: Blood, Smoker_3055


title: Blood, Smoker_3053


title: Blood, Smoker_3072


title: Blood, Non-smoker_3076


title: Blood, Non-smoker_3064


title: Blood, Non-smoker_3048


title: Blood, Smoker_3051


title: Blood, Non-smoker_3049


title: Blood, Non-smoker_3046


title: Blood, Non-smoker_3043


title: Blood, Non-smoker_3050


title: Blood, Smoker_3044


title: Blood, Smoker_3040


title: Blood, Non-smoker_3045


title: Blood, Smoker_3039


title: Blood, Non-smoker_3037


title: Blood, Non-smoker_3035


title: Blood, Non-smoker_3042


title: Blood, Non-smoker_3032


title: Blood, Non-smoker_3033


title: Blood, Non-smoker_3041


title: Blood, Non-smoker_3034


title: Blood, Non-smoker_3036


title: Blood, Non-smoker_3031


title: Blood, Non-smoker_3089


title: Blood, Non-smoker_3038


title: Blood, Smoker_3030


title: Blood, Non-smoker_3029


title: Blood, Smoker_3052


title: Blood, Smoker_3028


title: Blood, Non-smoker_3047


title: Blood, Smoker_3027


title: Blood, Smoker_3026


title: Blood, Non-smoker_3025


title: Blood, Non-smoker_3024


title: Blood, Smoker_3023


title: Blood, Non-smoker_3022


title: Blood, Non-smoker_3021


title: Blood, Smoker_3017


title: Blood, Non-smoker_3020


title: Blood, Non-smoker_3019


title: Blood, Non-smoker_3068


title: Blood, Smoker_3016


title: Blood, Smoker_3013


title: Blood, Non-smoker_3015


title: Blood, Smoker_3014


title: Blood, Smoker_3010


title: Blood, Smoker_3011


title: Blood, Non-smoker_3007


title: Blood, Non-smoker_3006


title: Blood, Non-smoker_3008


title: Blood, Smoker_3005


title: Blood, Smoker_3009


title: Blood, Non-smoker_3004


title: Blood, Smoker_3003


title: Blood, Smoker_3002


title: Blood, Non-smoker_3000


title: Blood, Non-smoker_3001


title: Blood, Non-smoker_2998


title: Blood, Non-smoker_2999


title: Blood, Smoker_2996


title: Blood, Non-smoker_3018


title: Blood, Non-smoker_2993


title: Blood, Non-smoker_2992


title: Blood, Non-smoker_2994


title: Blood, Smoker_2989


title: Blood, Non-smoker_2990


title: Blood, Non-smoker_2988


title: Blood, Smoker_2987


title: Blood, Smoker_3012


title: Blood, Smoker_2986


title: Blood, Smoker_2985


title: Blood, Non-smoker_2983


title: Blood, Non-smoker_2984


title: Blood, Smoker_2982


title: Blood, Smoker_2981


title: Blood, Non-smoker_2976


title: Blood, Smoker_2980


title: Blood, Smoker_2978


title: Blood, Smoker_2997


title: Blood, Non-smoker_2973


title: Blood, Non-smoker_2977


title: Blood, Smoker_2974


title: Blood, Non-smoker_2970


title: Blood, Non-smoker_2995


title: Blood, Non-smoker_2971


title: Blood, Smoker_2968


title: Blood, Non-smoker_2975


title: Blood, Non-smoker_2969


title: Blood, Smoker_2979


title: Blood, Non-smoker_2991


title: Blood, Smoker_2966


title: Blood, Smoker_2965


title: Blood, Smoker_2964


title: Blood, Non-smoker_2972


title: Blood, Smoker_2963


title: Blood, Non-smoker_2962


title: Blood, Non-smoker_2960


title: Blood, Smoker_2958


title: Blood, Non-smoker_2961


title: Blood, Smoker_2957


title: Blood, Non-smoker_2959


title: Blood, Smoker_2955


title: Blood, Smoker_2954


title: Blood, Non-smoker_2953


title: Blood, Smoker_2967


title: Blood, Non-smoker_2942


title: Blood, Non-smoker_2941


title: Blood, Smoker_2951


title: Blood, Non-smoker_2950


title: Blood, Non-smoker_2944


title: Blood, Smoker_2952


title: Blood, Non-smoker_2940


title: Blood, Non-smoker_2945


title: Blood, Smoker_2939


title: Blood, Smoker_2943


title: Blood, Non-smoker_2946


title: Blood, Non-smoker_2949


title: Blood, Non-smoker_2933


title: Blood, Smoker_2947


title: Blood, Smoker_2937


title: Blood, Smoker_2936


title: Blood, Non-smoker_2948


title: Blood, Non-smoker_2935


title: Blood, Non-smoker_2932


title: Blood, Non-smoker_2927


title: Blood, Non-smoker_2930


title: Blood, Non-smoker_2931


title: Blood, Smoker_2928


title: Blood, Smoker_2929


title: Blood, Smoker_2956


title: Blood, Non-smoker_2926


title: Blood, Non-smoker_2938


title: Blood, Non-smoker_2934


title: Blood, Non-smoker_2925


title: Blood, Non-smoker_2922


title: Blood, Non-smoker_2923


title: Blood, Smoker_2924


title: Blood, Non-smoker_2911


title: Blood, Non-smoker_2920


title: Blood, Non-smoker_2921


title: Blood, Smoker_2915


title: Blood, Non-smoker_2916


title: Blood, Non-smoker_2918


title: Blood, Non-smoker_2910


title: Blood, Non-smoker_2906


title: Blood, Non-smoker_2913


title: Blood, Non-smoker_2912


title: Blood, Non-smoker_2908


title: Blood, Non-smoker_2900


title: Blood, Non-smoker_2905


title: Blood, Non-smoker_2914


title: Blood, Non-smoker_2907


title: Blood, Non-smoker_2901


title: Blood, Non-smoker_2904


title: Blood, Non-smoker_2902


title: Blood, Non-smoker_2899


title: Blood, Non-smoker_2896


title: Blood, Non-smoker_2919


title: Blood, Non-smoker_2897


title: Blood, Non-smoker_2895


title: Blood, Non-smoker_2909


title: Blood, Non-smoker_2892


title: Blood, Non-smoker_2903


title: Blood, Non-smoker_2917


title: Blood, Non-smoker_2886


title: Blood, Non-smoker_2887


title: Blood, Non-smoker_2894


title: Blood, Non-smoker_2889


title: Blood, Non-smoker_2888


title: Blood, Smoker_2893


title: Blood, Smoker_2891


title: Blood, Smoker_2890


title: Blood, Smoker_2885


title: Blood, Smoker_2884


title: Blood, Non-smoker_2898


title: Blood, Non-smoker_2883


title: Blood, Non-smoker_2882


title: Blood, Non-smoker_2881


title: Blood, Non-smoker_2879


title: Blood, Non-smoker_2878


title: Blood, Non-smoker_2880


title: Blood, Non-smoker_2875


title: Blood, Smoker_2873


title: Blood, Smoker_2877


title: Blood, Non-smoker_2872


title: Blood, Non-smoker_2871


title: Blood, Non-smoker_2870


title: Blood, Non-smoker_2868


title: Blood, Non-smoker_2866


title: Blood, Non-smoker_2867


title: Blood, Smoker_2865


title: Blood, Non-smoker_2874


title: Blood, Smoker_2861


title: Blood, Non-smoker_2863


title: Blood, Non-smoker_2859


title: Blood, Non-smoker_2858


title: Blood, Non-smoker_2860


title: Blood, Smoker_2864


title: Blood, Smoker_2853


title: Blood, Non-smoker_2857


title: Blood, Non-smoker_2855


title: Blood, Non-smoker_2862


title: Blood, Non-smoker_2852


title: Blood, Non-smoker_2849


title: Blood, Non-smoker_2850


title: Blood, Non-smoker_2856


title: Blood, Non-smoker_2854


title: Blood, Non-smoker_2844


title: Blood, Non-smoker_2845


title: Blood, Non-smoker_2843


title: Blood, Non-smoker_2851


title: Blood, Non-smoker_2869


title: Blood, Smoker_2842


title: Blood, Non-smoker_2846


title: Blood, Smoker_2848


title: Blood, Non-smoker_2841


title: Blood, Smoker_2840


title: Blood, Smoker_2876


title: Blood, Non-smoker_2839


title: Blood, Non-smoker_2838


title: Blood, Non-smoker_2837


title: Blood, Non-smoker_2835


title: Blood, Smoker_2847


title: Blood, Non-smoker_2832


title: Blood, Non-smoker_2828


title: Blood, Non-smoker_2830


title: Blood, Non-smoker_2834


title: Blood, Non-smoker_2829


title: Blood, Non-smoker_2827


title: Blood, Non-smoker_2833


title: Blood, Non-smoker_2818


title: Blood, Smoker_2823


title: Blood, Non-smoker_2819


title: Blood, Non-smoker_2820


title: Blood, Non-smoker_2817


title: Blood, Non-smoker_2815


title: Blood, Non-smoker_2836


title: Blood, Smoker_2825


title: Blood, Smoker_2822


title: Blood, Non-smoker_2826


title: Blood, Non-smoker_2813


title: Blood, Non-smoker_2821


title: Blood, Non-smoker_2810


title: Blood, Non-smoker_2812


title: Blood, Smoker_2811


title: Blood, Smoker_2809


title: Blood, Non-smoker_2808


title: Blood, Smoker_2831


title: Blood, Smoker_2805


title: Blood, Non-smoker_2806


title: Blood, Non-smoker_2800


title: Blood, Non-smoker_2799


title: Blood, Non-smoker_2798


title: Blood, Non-smoker_2814


title: Blood, Non-smoker_2802


title: Blood, Smoker_2788


title: Blood, Smoker_2790


title: Blood, Smoker_2787


title: Blood, Non-smoker_2796


title: Blood, Smoker_2793


title: Blood, Smoker_2791


title: Blood, Smoker_2786


title: Blood, Smoker_2785


title: Blood, Smoker_2784


title: Blood, Non-smoker_2782


title: Blood, Non-smoker_2781


title: Blood, Non-smoker_2780


title: Blood, Non-smoker_2797


title: Blood, Non-smoker_2779


title: Blood, Non-smoker_2778


title: Blood, Non-smoker_2792


title: Blood, Non-smoker_2789


title: Blood, Non-smoker_2775


title: Blood, Smoker_2771


title: Blood, Smoker_2770


title: Blood, Smoker_2773


title: Blood, Smoker_2769


title: Blood, Smoker_2804


title: Blood, Non-smoker_2767


title: Blood, Smoker_2772


title: Blood, Non-smoker_2768


title: Blood, Non-smoker_2766


title: Blood, Non-smoker_2765


title: Blood, Smoker_2776


title: Blood, Non-smoker_2760


title: Blood, Smoker_2761


title: Blood, Smoker_2762


title: Blood, Non-smoker_2763


title: Blood, Non-smoker_2774


title: Blood, Non-smoker_2764


title: Blood, Smoker_2758


title: Blood, Non-smoker_2757


title: Blood, Non-smoker_2756


title: Blood, Non-smoker_2807


title: Blood, Non-smoker_2755


title: Blood, Smoker_2753


title: Blood, Non-smoker_2751


title: Blood, Smoker_2752


title: Blood, Non-smoker_2749


title: Blood, Non-smoker_2748


title: Blood, Non-smoker_2750


title: Blood, Non-smoker_2747


title: Blood, Non-smoker_2746


title: Blood, Non-smoker_2759


title: Blood, Non-smoker_2744


title: Blood, Non-smoker_2745


title: Blood, Smoker_2742


title: Blood, Smoker_2743


title: Blood, Non-smoker_2741


title: Blood, Non-smoker_2740


title: Blood, Smoker_2738


title: Blood, Smoker_2739


title: Blood, Non-smoker_2777


title: Blood, Smoker_2754


title: Blood, Smoker_2737


title: Blood, Non-smoker_2736


title: Blood, Non-smoker_2734


title: Blood, Non-smoker_2733


title: Blood, Smoker_2735


title: Blood, Smoker_2730


title: Blood, Smoker_2731


title: Blood, Smoker_2732


title: Blood, Non-smoker_2794


title: Blood, Non-smoker_2728


title: Blood, Non-smoker_2727


title: Blood, Non-smoker_2726


title: Blood, Non-smoker_2724


title: Blood, Smoker_2720


title: Blood, Smoker_2721


title: Blood, Smoker_2725


title: Blood, Smoker_2719


title: Blood, Non-smoker_2723


title: Blood, Smoker_2722


title: Blood, Smoker_2717


title: Blood, Non-smoker_2729


title: Blood, Non-smoker_2716


title: Blood, Smoker_2718


title: Blood, Non-smoker_2714


title: Blood, Smoker_2715


title: Blood, Non-smoker_2712


title: Blood, Non-smoker_2713


title: Blood, Smoker_2783


title: Blood, Non-smoker_2704


title: Blood, Smoker_2711


title: Blood, Smoker_2710


title: Blood, Non-smoker_2703


title: Blood, Non-smoker_2708


title: Blood, Non-smoker_2706


title: Blood, Non-smoker_2709


title: Blood, Smoker_2705


title: Blood, Non-smoker_2702


title: Blood, Non-smoker_2699


title: Blood, Smoker_2701


title: Blood, Non-smoker_2700


title: Blood, Smoker_2698


title: Blood, Smoker_2803


title: Blood, Non-smoker_2795


title: Blood, Non-smoker_2707


title: Blood, Smoker_2697


title: Blood, Non-smoker_2695


title: Blood, Non-smoker_2688


title: Blood, Smoker_2692


title: Blood, Smoker_2693


title: Blood, Smoker_2691


title: Blood, Smoker_2690


title: Blood, Non-smoker_2689


title: Blood, Smoker_2686


title: Blood, Smoker_2684


title: Blood, Non-smoker_2685


title: Blood, Smoker_2681


title: Blood, Smoker_2682


title: Blood, Smoker_2683


title: Blood, Smoker_2696


title: Blood, Non-smoker_2678


title: Blood, Non-smoker_2674


title: Blood, Non-smoker_2676


title: Blood, Non-smoker_2675


title: Blood, Non-smoker_2677


title: Blood, Non-smoker_2673


title: Blood, Smoker_2680


title: Blood, Non-smoker_2671


title: Blood, Smoker_2694


title: Blood, Non-smoker_2670


title: Blood, Smoker_2816


title: Blood, Non-smoker_2687


title: Blood, Non-smoker_2679


title: Blood, Smoker_2669


title: Blood, Smoker_2664


title: Blood, Smoker_2666


title: Blood, Non-smoker_2665


title: Blood, Smoker_2667


title: Blood, Smoker_2661


title: Blood, Non-smoker_2662


title: Blood, Smoker_2659


title: Blood, Non-smoker_2668


title: Blood, Smoker_2660


title: Blood, Non-smoker_2657


title: Blood, Smoker_2656


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title: Blood, Non-smoker_2654


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title: Blood, Non-smoker_2649


title: Blood, Non-smoker_2647


title: Blood, Non-smoker_2648


title: Blood, Non-smoker_2646


title: Blood, Smoker_2645


title: Blood, Non-smoker_2663


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title: Blood, Non-smoker_1976


title: Blood, Non-smoker_1963


title: Blood, Non-smoker_1975


title: Blood, Non-smoker_1960


title: Blood, Smoker_1990


title: Blood, Non-smoker_1971


title: Blood, Smoker_1969


title: Blood, Smoker_1970


title: Blood, Non-smoker_1959


title: Blood, Smoker_1968


title: Blood, Smoker_1967


title: Blood, Non-smoker_1966


title: Blood, Smoker_1955


title: Blood, Smoker_1954


title: Blood, Non-smoker_1961


title: Blood, Non-smoker_1958


title: Blood, Non-smoker_1953


title: Blood, Smoker_1956


title: Blood, Smoker_1952


title: Blood, Non-smoker_1951


title: Blood, Smoker_1950


title: Blood, Non-smoker_1947


title: Blood, Non-smoker_1949


title: Blood, Non-smoker_1948


title: Blood, Smoker_1964


title: Blood, Non-smoker_1962


title: Blood, Smoker_1946


title: Blood, Smoker_1945


title: Blood, Non-smoker_1944


title: Blood, Smoker_1943


title: Blood, Non-smoker_1940


title: Blood, Non-smoker_1942


title: Blood, Non-smoker_1941


title: Blood, Non-smoker_1939


title: Blood, Non-smoker_1938


title: Blood, Non-smoker_1936


title: Blood, Non-smoker_1935


title: Blood, Non-smoker_1957


title: Blood, Smoker_1934


title: Blood, Non-smoker_1983


title: Blood, Smoker_1933


title: Blood, Smoker_1932


title: Blood, Smoker_1931


title: Blood, Smoker_1930


title: Blood, Smoker_1928


title: Blood, Non-smoker_1926


title: Blood, Smoker_1929


title: Blood, Non-smoker_1925


title: Blood, Smoker_1921


title: Blood, Non-smoker_1922


title: Blood, Non-smoker_1920


title: Blood, Non-smoker_1923


title: Blood, Non-smoker_1918


title: Blood, Non-smoker_1924


title: Blood, Smoker_1919


title: Blood, Non-smoker_1916


title: Blood, Non-smoker_1917


title: Blood, Non-smoker_1915


title: Blood, Non-smoker_1912


title: Blood, Smoker_1937


title: Blood, Non-smoker_1913


title: Blood, Non-smoker_1914


title: Blood, Non-smoker_1911


title: Blood, Non-smoker_1908


title: Blood, Non-smoker_1909


title: Blood, Non-smoker_1907


title: Blood, Smoker_1905


title: Blood, Non-smoker_1904


title: Blood, Smoker_1903


title: Blood, Non-smoker_1906


title: Blood, Non-smoker_1927


title: Blood, Smoker_1902


title: Blood, Smoker_1898


title: Blood, Smoker_1901


title: Blood, Non-smoker_1897


title: Blood, Smoker_1894


title: Blood, Non-smoker_1899


title: Blood, Non-smoker_1896


title: Blood, Non-smoker_1900


title: Blood, Non-smoker_1893


title: Blood, Smoker_1889


title: Blood, Smoker_1890


title: Blood, Smoker_1892


title: Blood, Non-smoker_1887


title: Blood, Smoker_1895


title: Blood, Non-smoker_1910


title: Blood, Non-smoker_1888


title: Blood, Non-smoker_1885


title: Blood, Non-smoker_1884


title: Blood, Non-smoker_1886


title: Blood, Non-smoker_1882


title: Blood, Smoker_1881


title: Blood, Smoker_1879


title: Blood, Non-smoker_1878


title: Blood, Smoker_1876


title: Blood, Smoker_1875


title: Blood, Non-smoker_1877


title: Blood, Smoker_1873


title: Blood, Non-smoker_1874


title: Blood, Non-smoker_1872


title: Blood, Non-smoker_1871


title: Blood, Non-smoker_1870


title: Blood, Smoker_1867


title: Blood, Smoker_1891


title: Blood, Smoker_1866


title: Blood, Non-smoker_1869


title: Blood, Non-smoker_1868


title: Blood, Smoker_1865


title: Blood, Smoker_1883


title: Blood, Non-smoker_1861


title: Blood, Non-smoker_1863


title: Blood, Non-smoker_1856


title: Blood, Smoker_1864


title: Blood, Non-smoker_1860


title: Blood, Smoker_1859


title: Blood, Smoker_1855


title: Blood, Smoker_1880


title: Blood, Smoker_1854


title: Blood, Non-smoker_1857


title: Blood, Non-smoker_1852


title: Blood, Smoker_1849


title: Blood, Smoker_1848


title: Blood, Smoker_1851


title: Blood, Smoker_1846


title: Blood, Non-smoker_1843


title: Blood, Smoker_1842


title: Blood, Smoker_1841


title: Blood, Non-smoker_1862


title: Blood, Non-smoker_1839


title: Blood, Non-smoker_1858


title: Blood, Smoker_1838


title: Blood, Smoker_1836


title: Blood, Smoker_1837


title: Blood, Non-smoker_1853


title: Blood, Non-smoker_1834


title: Blood, Smoker_1830


title: Blood, Non-smoker_1832


title: Blood, Non-smoker_1833


title: Blood, Non-smoker_1829


title: Blood, Smoker_1850


title: Blood, Smoker_1828


title: Blood, Non-smoker_1826


title: Blood, Smoker_1847


title: Blood, Non-smoker_1823


title: Blood, Non-smoker_1825


title: Blood, Non-smoker_1844


title: Blood, Non-smoker_1822


title: Blood, Smoker_1821


title: Blood, Smoker_1820


title: Blood, Non-smoker_1819


title: Blood, Non-smoker_1840


title: Blood, Non-smoker_1818


title: Blood, Smoker_1817


title: Blood, Smoker_1814


title: Blood, Non-smoker_1835


title: Blood, Smoker_1815


title: Blood, Smoker_1816


title: Blood, Smoker_1831


title: Blood, Non-smoker_1813


title: Blood, Smoker_1845


title: Blood, Non-smoker_1810


title: Blood, Smoker_1827


title: Blood, Non-smoker_1807


title: Blood, Smoker_1811


title: Blood, Smoker_1812


title: Blood, Non-smoker_1806


title: Blood, Smoker_1809


title: Blood, Non-smoker_1804


title: Blood, Smoker_1824


title: Blood, Non-smoker_1803


title: Blood, Smoker_1805


title: Blood, Smoker_1802


title: Blood, Smoker_1801


title: Blood, Smoker_1800


title: Blood, Smoker_1797


title: Blood, Non-smoker_1798


title: Blood, Non-smoker_1799


title: Blood, Smoker_1794


title: Blood, Non-smoker_1796


title: Blood, Smoker_1795


title: Blood, Non-smoker_1793


title: Blood, Smoker_1791


title: Blood, Smoker_1788


title: Blood, Smoker_1790


title: Blood, Smoker_1787


title: Blood, Non-smoker_1789


title: Blood, Smoker_1808


title: Blood, Non-smoker_1784


title: Blood, Smoker_1780


title: Blood, Non-smoker_1782


title: Blood, Smoker_1783


title: Blood, Smoker_1779


title: Blood, Non-smoker_1778


title: Blood, Smoker_1777


title: Blood, Smoker_1781


title: Blood, Non-smoker_1785


title: Blood, Smoker_1776


title: Blood, Non-smoker_1775


title: Blood, Non-smoker_1774


title: Blood, Non-smoker_1773


title: Blood, Smoker_1792


title: Blood, Non-smoker_1770


title: Blood, Smoker_1771


title: Blood, Non-smoker_1786


title: Blood, Non-smoker_1769


title: Blood, Smoker_1768


title: Blood, Smoker_1767


title: Blood, Non-smoker_1766


title: Blood, Smoker_1765


title: Blood, Smoker_1764


title: Blood, Smoker_1762


title: Blood, Non-smoker_1763


title: Blood, Smoker_1760


title: Blood, Smoker_1761


title: Blood, Non-smoker_1759


title: Blood, Smoker_1758


title: Blood, Non-smoker_1756


title: Blood, Smoker_1752


title: Blood, Smoker_1757


title: Blood, Non-smoker_1750


title: Blood, Non-smoker_1772


title: Blood, Smoker_1751


title: Blood, Non-smoker_1754


title: Blood, Smoker_1748


title: Blood, Non-smoker_1749


title: Blood, Smoker_1747


title: Blood, Non-smoker_1746


title: Blood, Non-smoker_1744


title: Blood, Non-smoker_1740


title: Blood, Smoker_1741


title: Blood, Smoker_1742


title: Blood, Smoker_1745


title: Blood, Smoker_1743


title: Blood, Smoker_1739


title: Blood, Smoker_1738


title: Blood, Smoker_1737


title: Blood, Smoker_1734


title: Blood, Non-smoker_1735


title: Blood, Non-smoker_1753


title: Blood, Smoker_1731


title: Blood, Smoker_1733


title: Blood, Smoker_1732


title: Blood, Non-smoker_1755