title: Cerebral Cortex, mouse17K-A, 39 AMC55 F 712 C

description: 39 AMC55 F 712 C


title: Striatum, mouse17K-B, 33 AMC51 M 194_ad-lib

status: Public on Dec 28 2007

type: RNA

city: Baltimore

first: Supriyo

last: De

state: Maryland

address: 251 Bayview Blvd

department: Laboratory of Genetics and Genomics

country: USA

institute: NIA-IRP, NIH

description: 33 AMC51 M 194 A

hyb_protocol: Radiolabeled cDNA was denatured at 95 degrees C for 7 minutes and added to 12 mls of Invitrogen microhyb containing 10% dextran sulfate with Cot-1 DNA (Invitrogen) and Poly-A DNA oligos, (Sigma) added as blocking agents.  Arrays were hybridized overnight at 55 degrees C to mouse 17K-B cDNA filters.  The hybridized filters were quickly rinsed once and then washed twice at 65 degrees placed onto steel plates covered with plastic wrap and exposed to Kodak storage Phosphorimaging screens for 3-4 days.

scan_protocol: Imaging screens were scanned at 50um resolution in a Storm Phosphorimager.

data_processing: All data was extracted using ArrayPro Software and processed in Excel spreadsheets using Z normalization of each array.  Differently expressed genes were identified between the different sample groups by z-ratio analysis using an in-house microarray analysis program.

molecule: total RNA

organism: Mus musculus

source_name: Mus musculus Striatum

label_protocol: ~5 ug of total RNA was radiolabeled with 33P-dCTP using Superscript II from Invitrogen.  Labeled cDNA was separated from unincorporated probe and small nucleotides using a Bio-Rad size exclusion column, Bio-Spin 30 with SSC. 1ul of each sample was counted in a scintillation counter and the remainder was used for hybridization.

extract_protocol: Qiagen RNeasy Mini Kit protocol for animal tissues after disruption using a mini-bead beater-8 and 1.0mm glass beads.

treatment_protocol: 100% regular feed, ad libitum until age of sacrifice

tag: Strain

value: C57BL/6, Tissue:Striatum

tag: Gender

value: male, Age:194 days Diet:Ad lib


title: Striatum, mouse17K-B, 30 AMC198 F 21_ad-lib

description: 30 AMC198 F 21 A

value: female, Age:21 days Diet:Ad lib


title: Striatum, mouse17K-B, 29 AMC193 M 19_ad-lib

description: 29 AMC193 M 19 A

value: male, Age:19 days Diet:Ad lib


title: Striatum, mouse17K-B, 31 AMC31 M 194 C

description: 31 AMC31 M 194 C

treatment_protocol: 100% regular feed to 13 weeks of age, 90% fortified feed for 1 week, 75% fortified feed for 1 week, then 60% fortified feed until the age of sacrifice

value: male, Age:194 days Diet:Caloric restriction


title: Cerebral Cortex, mouse17K-A, 40 AMC118 M 719_ad-lib

description: 40 AMC118 M 719 A

hyb_protocol: Radiolabeled cDNA was denatured at 95 degrees C for 7 minutes and added to 12 mls of Invitrogen microhyb containing 10% dextran sulfate with Cot-1 DNA (Invitrogen) and Poly-A DNA oligos, (Sigma) added as blocking agents.  Arrays were hybridized overnight at 55 degrees C to mouse 17K-A cDNA filters.  The hybridized filters were quickly rinsed once and then washed twice at 65 degrees placed onto steel plates covered with plastic wrap and exposed to Kodak storage Phosphorimaging screens for 3-4 days.

source_name: Mus musculus Cerebral Cortex

value: C57BL/6, Tissue:Cerebral Cortex

value: male, Age:719 days Diet:Ad lib


title: Cerebral Cortex, mouse17K-A, 38 AMC86 F 476_ad-lib

description: 38 AMC86 F 476 A

value: female, Age:476 days Diet:Ad lib


title: Striatum, mouse17K-B, 28 AMC73 F 714_ad-lib

description: 28 AMC73 F 714 A

value: female, Age:714 days Diet:Ad lib


title: Striatum, mouse17K-B, 25 AMC208 M 673 C

description: 25 AMC208 M 673 C

value: male, Age:673 days Diet:Caloric restriction


title: Cerebral Cortex, mouse17K-A, 36 AMC28 F 499 C

description: 36 AMC28 F 499 C

value: female, Age:499 days Diet:Caloric restriction


title: Cerebral Cortex, mouse17K-A, 37 AMC125 M 481_ad-lib

description: 37 AMC125 M 481 A

value: male, Age:481 days Diet:Ad lib


title: Cerebral Cortex, mouse17K-A, 35 AMC101 M 485 C

description: 35 AMC101 M 485 C

value: male, Age:485 days Diet:Caloric restriction


title: Striatum, mouse17K-B, 27 AMC76 M 714_ad-lib

description: 01 AMC185 M  43 A

value: male, Age:714 days Diet:Ad lib


title: Cerebral Cortex, mouse17K-A, 41 AMC74 F 712_ad-lib

description: 41 AMC74 F 712 A

value: female, Age:712 days Diet:Ad lib


title: Striatum, mouse17K-B, 24 AMC48 F 499_ad-lib

description: 24 AMC48 F 499 A

value: female, Age:499 days Diet:Ad lib


title: Cerebral Cortex, mouse17K-A, 34 AMC53 F 194_ad-lib

description: 34 AMC53 F 194 A

value: female, Age:194 days Diet:Ad lib


title: Striatum, mouse17K-B, 23 AMC80 M 476_ad-lib

description: 23 AMC80 M 476 A

value: C57BL/6, Tissue:Striatum

value: male, Age:476 days Diet:Ad lib


title: Striatum, mouse17K-B, 32 AMC113 F 180 C

description: 32 AMC113 F 180 C

value: female, Age:180 days Diet:Caloric restriction


title: Striatum, mouse17K-B, 22 AMC27 F 498 C

description: 22 AMC27 F 498 C

value: female, Age:498 days Diet:Caloric restriction


title: Striatum, mouse17K-B, 21 AMC58 M 472 C

description: 21 AMC58 M 472 C

value: male, Age:472 days Diet:Caloric restriction


title: Striatum, mouse17K-B, 20 AMC52 F 193_ad-lib

description: 20 AMC52 F 193 A

value: female, Age:193 days Diet:Ad lib


title: Striatum, mouse17K-B, 17 AMC30 M 193 C

description: 17 AMC30 M 193 C

value: male, Age:193 days Diet:Caloric restriction


title: Striatum, mouse17K-B, 18 AMC34 F 194 C

description: 18 AMC34 F 194 C

value: female, Age:194 days Diet:Caloric restriction


title: Striatum, mouse17K-B, 16 AMC197 F 19_ad-lib

description: 16 AMC197 F 19 A

value: female, Age:19 days Diet:Ad lib


title: Striatum, mouse17K-B, 19 AMC50 M 193_ad-lib

description: 19 AMC50 M 193 A

value: male, Age:193 days Diet:Ad lib


title: Cerebral Cortex, mouse17K-A, 33 AMC51 M 194_ad-lib

description: 33 AMC51 M 194 A

value: C57BL/6, Tissue:Cerebral Cortex

value: male, Age:194 days Diet:Ad lib


title: Cerebral Cortex, mouse17K-A, 32 AMC113 F 180 C

description: 32 AMC113 F 180 C


title: Striatum, mouse17K-B, 14 AMC72 F 716_ad-lib

description: 14 AMC72 F 716 A

value: female, Age:716 days Diet:Ad lib


title: Striatum, mouse17K-B, 26 AMC25 F 743 C

description: 26 AMC25 F 743 C

value: female, Age:743 days Diet:Caloric restriction


title: Striatum, mouse17K-B, 15 AMC186 M 44_ad-lib

description: 15 AMC186 M 44 A

value: male, Age:44 days Diet:Ad lib


title: Striatum, mouse17K-B, 11 AMC207 M 673 C

description: 11 AMC207 M 673 C

value: male, Age:673 days Diet:Caloric restriction


title: Striatum, mouse17K-B, 13 AMC36 M 743_ad-lib

description: 13 AMC36 M 743 A

value: male, Age:743 days Diet:Ad lib


title: Striatum, mouse17K-B, 07 AMC57 M 474 C

description: 07 AMC57 M 474 C

value: C57BL/6, Tissue:Striatum

value: male, Age:474 days Diet:Caloric restriction


title: Cerebral Cortex, mouse17K-A, 31 AMC31 M 194 C

description: 31 AMC31 M 194 C

value: male, Age:194 days Diet:Caloric restriction


title: Striatum, mouse17K-B, 12 AMC1 F 736 C

description: 12 AMC1 F 736 C

value: female, Age:736 days Diet:Caloric restriction


title: Striatum, mouse17K-B, 09 AMC43 M 498_ad-lib

description: 09 AMC43 M 498 A

value: male, Age:498 days Diet:Ad lib


title: Striatum, mouse17K-B, 10 AMC46 F 498_ad-lib

description: 10 AMC46 F 498 A

value: female, Age:498 days Diet:Ad lib


title: Striatum, mouse17K-B, 08 AMC4 F 491 C

description: 08 AMC4 F 491 C

value: female, Age:491 days Diet:Caloric restriction


title: Striatum, mouse17K-B, 06 AMC23 F 186_ad-lib

description: 06 AMC23 F 186 A

value: female, Age:186 days Diet:Ad lib


title: Cerebral Cortex, mouse17K-A, 29 AMC193 M 19_ad-lib

description: 29 AMC193 M 19 A

value: male, Age:19 days Diet:Ad lib


title: Striatum, mouse17K-B, 05 AMC21 M 186_ad-lib

description: 05 AMC21 M 186 A

value: male, Age:186 days Diet:Ad lib


title: Cerebral Cortex, mouse17K-A, 30 AMC198 F 21_ad-lib

description: 30 AMC198 F 21 A

value: C57BL/6, Tissue:Cerebral Cortex

value: female, Age:21 days Diet:Ad lib


title: Cerebral Cortex, mouse17K-A, 27 AMC76 M 714_ad-lib

description: 01 AMC185 M  43 A

value: male, Age:714 days Diet:Ad lib


title: Cerebral Cortex, mouse17K-A, 25 AMC208 M 673 C

description: 25 AMC208 M 673 C


title: Striatum, mouse17K-B, 03 AMC7 M 186 C

description: 03 AMC7 M 186 C

value: male, Age:186 days Diet:Caloric restriction


title: Striatum, mouse17K-B, 02 AMC196 F 17_ad-lib

description: 02 AMC196 F 17 A

value: female, Age:17 days Diet:Ad lib


title: Striatum, mouse17K-B, 04 AMC33 F 193 C

description: 04 AMC33 F 193 C

value: female, Age:193 days Diet:Caloric restriction


title: Cerebral Cortex, mouse17K-A, 26 AMC25 F 743 C

description: 26 AMC25 F 743 C

value: female, Age:743 days Diet:Caloric restriction


title: Cerebral Cortex, mouse17K-A, 24 AMC48 F 499_ad-lib

description: 24 AMC48 F 499 A

value: female, Age:499 days Diet:Ad lib


title: Striatum, mouse17K-B, 01 AMC185 M 43_ad-lib

description: 01 AMC185 M 43 A

value: C57BL/6, Tissue:Striatum

value: male, Age:43 days Diet:Ad lib


title: Spinal Cord, mouse17K-B, 67 AMC174 F 704_ad-lib

description: 67 AMC174 F 704 A

source_name: Mus musculus Spinal Cord

value: C57BL/6, Tissue:Spinal Cord

value: female, Age:704 days Diet:Ad lib


title: Cerebral Cortex, mouse17K-A, 23 AMC80 M 476_ad-lib

description: 23 AMC80 M 476 A

value: male, Age:476 days Diet:Ad lib


title: Cerebral Cortex, mouse17K-A, 28 AMC73 F 714_ad-lib

description: 28 AMC73 F 714 A

value: female, Age:714 days Diet:Ad lib


title: Spinal Cord, mouse17K-B, 64 AMC131 F 479_ad-lib

description: 64 AMC131 F 479 A

value: female, Age:479 days Diet:Ad lib


title: Spinal Cord, mouse17K-B, 63 AMC170 M 495_ad-lib

description: 63 AMC170 M 495 A

value: male, Age:495 days Diet:Ad lib


title: Cerebral Cortex, mouse17K-A, 21 AMC58 M 472 C

description: 21 AMC58 M 472 C

value: male, Age:472 days Diet:Caloric restriction


title: Spinal Cord, mouse17K-B, 57 AMC244 F 33_ad-lib

description: 57 AMC244 F 33 A

value: female, Age:33 days Diet:Ad lib


title: Cerebral Cortex, mouse17K-A, 22 AMC27 F 498 C

description: 22 AMC27 F 498 C

value: C57BL/6, Tissue:Cerebral Cortex

value: female, Age:498 days Diet:Caloric restriction


title: Spinal Cord, mouse17K-B, 65 AMC150 F 703 C

description: 65 AMC150 F 703 C

value: female, Age:703 days Diet:Caloric restriction


title: Cerebral Cortex, mouse17K-A, 17 AMC30 M 193 C

description: 17 AMC30 M 193 C

value: male, Age:193 days Diet:Caloric restriction


title: Spinal Cord, mouse17K-B, 61 AMC209 M 482 C

description: 61 AMC209 M 482 C

value: male, Age:482 days Diet:Caloric restriction


title: Spinal Cord, mouse17K-B, 66 AMC163 M 733_ad-lib

description: 66 AMC163 M 733 A

value: male, Age:733 days Diet:Ad lib


title: Spinal Cord, mouse17K-B, 52 AMC130 F 481_ad-lib

description: 52 AMC130 F 481 A

value: female, Age:481 days Diet:Ad lib


title: Cerebral Cortex, mouse17K-A, 18 AMC34 F 194 C

description: 18 AMC34 F 194 C

value: female, Age:194 days Diet:Caloric restriction


title: Spinal Cord, mouse17K-B, 54 AMC119 M 730_ad-lib

description: 54 AMC119 M 730 A

value: male, Age:730 days Diet:Ad lib


title: Spinal Cord, mouse17K-B, 50 AMC217 M 177 C

description: 50 AMC217 M 177 C

value: C57BL/6, Tissue:Spinal Cord

value: male, Age:177 days Diet:Caloric restriction


title: Spinal Cord, mouse17K-B, 56 AMC239 M 40_ad-lib

description: 56 AMC239 M 40 A

value: male, Age:40 days Diet:Ad lib


title: Spinal Cord, mouse17K-B, 59 AMC135 M 174_ad-lib

description: 59 AMC135 M 174 A

value: male, Age:174 days Diet:Ad lib


title: Spinal Cord, mouse17K-B, 55 AMC167 F 733_ad-lib

description: 55 AMC167 F 733 A

value: female, Age:733 days Diet:Ad lib


title: Spinal Cord, mouse17K-B, 53 AMC56 F 714 C

description: 53 AMC56 F 714 C

value: female, Age:714 days Diet:Caloric restriction


title: Spinal Cord, mouse17K-B, 48 AMC104 F 485 C

description: 48 AMC104 F 485 C

value: female, Age:485 days Diet:Caloric restriction


title: Spinal Cord, mouse17K-B, 51 AMC127 M 485_ad-lib

description: 51 AMC127 M 485 A

value: male, Age:485 days Diet:Ad lib


title: Cerebral Cortex, mouse17K-A, 13 AMC36 M 743_ad-lib

description: 13 AMC36 M 743 A

value: C57BL/6, Tissue:Cerebral Cortex

value: male, Age:743 days Diet:Ad lib


title: Cerebral Cortex, mouse17K-A, 16 AMC197 F 19_ad-lib

description: 16 AMC197 F 19 A

value: female, Age:19 days Diet:Ad lib


title: Spinal Cord, mouse17K-B, 60 AMC137 F 180_ad-lib

description: 60 AMC137 F 180 A

value: female, Age:180 days Diet:Ad lib


title: Spinal Cord, mouse17K-B, 49 AMC245 M 673 C

description: 49 AMC245 M 673 C

value: male, Age:673 days Diet:Caloric restriction


title: Cerebral Cortex, mouse17K-A, 15 AMC186 M 44_ad-lib

description: 15 AMC186 M 44 A

value: male, Age:44 days Diet:Ad lib


title: Cerebral Cortex, mouse17K-A, 14 AMC72 F 716_ad-lib

description: 14 AMC72 F 716 A

value: female, Age:716 days Diet:Ad lib


title: Spinal Cord, mouse17K-B, 47 AMC148 M 495 C

description: 47 AMC148 M 495 C

value: male, Age:495 days Diet:Caloric restriction


title: Spinal Cord, mouse17K-B, 46 AMC92 F 169_ad-lib

description: 46 AMC92 F 169 A

value: female, Age:169 days Diet:Ad lib


title: Cerebral Cortex, mouse17K-A, 20 AMC52 F 193_ad-lib

description: 20 AMC52 F 193 A

value: female, Age:193 days Diet:Ad lib


title: Spinal Cord, mouse17K-B, 58 AMC158 F 190 C

description: 58 AMC158 F 190 C

value: C57BL/6, Tissue:Spinal Cord

value: female, Age:190 days Diet:Caloric restriction


title: Cerebral Cortex, mouse17K-A, 12 AMC1 F 736 C

description: 12 AMC1 F 736 C

value: female, Age:736 days Diet:Caloric restriction


title: Cerebral Cortex, mouse17K-A, 11 AMC207 M 673 C

description: 11 AMC207 M 673 C


title: Spinal Cord, mouse17K-B, 42 AMC194 M 17_ad-lib

description: 42 AMC194 M 17 A

value: male, Age:17 days Diet:Ad lib


title: Spinal Cord, mouse17K-B, 43 AMC241 F 40_ad-lib

description: 43 AMC241 F 40 A

value: female, Age:40 days Diet:Ad lib


title: Spinal Cord, mouse17K-B, 44 AMC115 F 174 C

description: 44 AMC115 F 174 C

value: female, Age:174 days Diet:Caloric restriction


title: Spinal Cord, mouse17K-B, 41 AMC74 F 712_ad-lib

description: 41 AMC74 F 712 A

value: female, Age:712 days Diet:Ad lib


title: Spinal Cord, mouse17K-B, 45 AMC133 M 180_ad-lib

description: 45 AMC133 M 180 A

value: male, Age:180 days Diet:Ad lib


title: Spinal Cord, mouse17K-B, 39 AMC55 F 712 C

description: 39 AMC55 F 712 C

value: female, Age:712 days Diet:Caloric restriction


title: Spinal Cord, mouse17K-B, 38 AMC86 F 476_ad-lib

description: 38 AMC86 F 476 A

value: female, Age:476 days Diet:Ad lib


title: Spinal Cord, mouse17K-B, 37 AMC125 M 481_ad-lib

description: 37 AMC125 M 481 A

value: male, Age:481 days Diet:Ad lib


title: Spinal Cord, mouse17K-B, 40 AMC118 M 719_ad-lib

description: 40 AMC118 M 719 A

value: male, Age:719 days Diet:Ad lib


title: Spinal Cord, mouse17K-B, 36 AMC28 F 499 C

description: 36 AMC28 F 499 C

value: female, Age:499 days Diet:Caloric restriction


title: Cerebral Cortex, mouse17K-A, 09 AMC43 M 498_ad-lib

description: 09 AMC43 M 498 A

value: C57BL/6, Tissue:Cerebral Cortex

value: male, Age:498 days Diet:Ad lib


title: Cerebral Cortex, mouse17K-A, 10 AMC46 F 498_ad-lib

description: 10 AMC46 F 498 A

value: female, Age:498 days Diet:Ad lib


title: Spinal Cord, mouse17K-B, 35 AMC101 M 485 C

description: 35 AMC101 M 485 C

value: male, Age:485 days Diet:Caloric restriction


title: Spinal Cord, mouse17K-B, 62 AMC152 F 495 C

description: 62 AMC152 F 495 C

value: C57BL/6, Tissue:Spinal Cord

value: female, Age:495 days Diet:Caloric restriction


title: Spinal Cord, mouse17K-B, 34 AMC53 F 194_ad-lib

description: 34 AMC53 F 194 A

value: female, Age:194 days Diet:Ad lib


title: Spinal Cord, mouse17K-B, 33 AMC51 M 194_ad-lib

description: 33 AMC51 M 194 A

value: male, Age:194 days Diet:Ad lib


title: Cerebral Cortex, mouse17K-A, 19 AMC50 M 193_ad-lib

description: 19 AMC50 M 193 A

value: male, Age:193 days Diet:Ad lib


title: Cerebral Cortex, mouse17K-A, 06 AMC23 F 186_ad-lib

description: 06 AMC23 F 186 A

value: female, Age:186 days Diet:Ad lib


title: Spinal Cord, mouse17K-B, 32 AMC113 F 180 C

description: 32 AMC113 F 180 C

value: female, Age:180 days Diet:Caloric restriction


title: Spinal Cord, mouse17K-B, 30 AMC198 F 21_ad-lib

description: 30 AMC198 F 21 A

value: female, Age:21 days Diet:Ad lib


title: Spinal Cord, mouse17K-B, 31 AMC31 M 194 C

description: 31 AMC31 M 194 C

value: male, Age:194 days Diet:Caloric restriction


title: Spinal Cord, mouse17K-B, 28 AMC73 F 714_ad-lib

description: 28 AMC73 F 714 A

value: female, Age:714 days Diet:Ad lib


title: Cerebral Cortex, mouse17K-A, 07 AMC57 M 474 C

description: 07 AMC57 M 474 C

value: male, Age:474 days Diet:Caloric restriction


title: Spinal Cord, mouse17K-B, 27 AMC76 M 714_ad-lib

description: 01 AMC185 M  43 A

value: male, Age:714 days Diet:Ad lib


title: Cerebral Cortex, mouse17K-A, 05 AMC21 M 186_ad-lib

description: 05 AMC21 M 186 A

value: male, Age:186 days Diet:Ad lib


title: Cerebral Cortex, mouse17K-A, 02 AMC196 F 17_ad-lib

description: 02 AMC196 F 17 A

value: C57BL/6, Tissue:Cerebral Cortex

value: female, Age:17 days Diet:Ad lib


title: Spinal Cord, mouse17K-B, 29 AMC193 M 19_ad-lib

description: 29 AMC193 M 19 A

value: male, Age:19 days Diet:Ad lib


title: Cerebral Cortex, mouse17K-A, 03 AMC7 M 186 C

description: 03 AMC7 M 186 C

value: male, Age:186 days Diet:Caloric restriction


title: Cerebral Cortex, mouse17K-A, 08 AMC4 F 491 C

description: 08 AMC4 F 491 C

value: female, Age:491 days Diet:Caloric restriction


title: Spinal Cord, mouse17K-B, 25 AMC208 M 673 C

description: 25 AMC208 M 673 C

value: C57BL/6, Tissue:Spinal Cord

value: male, Age:673 days Diet:Caloric restriction


title: Spinal Cord, mouse17K-B, 23 AMC80 M 476_ad-lib

description: 23 AMC80 M 476 A

value: male, Age:476 days Diet:Ad lib


title: Spinal Cord, mouse17K-B, 21 AMC58 M 472 C

description: 21 AMC58 M 472 C

value: male, Age:472 days Diet:Caloric restriction


title: Spinal Cord, mouse17K-B, 17 AMC30 M 193 C

description: 17 AMC30 M 193 C

value: male, Age:193 days Diet:Caloric restriction


title: Spinal Cord, mouse17K-B, 18 AMC34 F 194 C

description: 18 AMC34 F 194 C

value: female, Age:194 days Diet:Caloric restriction


title: Cerebral Cortex, mouse17K-A, 04 AMC33 F 193 C

description: 04 AMC33 F 193 C

value: female, Age:193 days Diet:Caloric restriction


title: Cerebral Cortex, mouse17K-A, 01 AMC185 M 43_ad-lib

description: 01 AMC185 M 43 A

value: male, Age:43 days Diet:Ad lib


title: Spinal Cord, mouse17K-B, 26 AMC25 F 743 C

description: 26 AMC25 F 743 C

value: female, Age:743 days Diet:Caloric restriction


title: Spinal Cord, mouse17K-B, 24 AMC48 F 499_ad-lib

description: 24 AMC48 F 499 A

value: female, Age:499 days Diet:Ad lib


title: Spinal Cord, mouse17K-B, 20 AMC52 F 193_ad-lib

description: 20 AMC52 F 193 A

value: female, Age:193 days Diet:Ad lib


title: Spinal Cord, mouse17K-B, 19 AMC50 M 193_ad-lib

description: 19 AMC50 M 193 A

value: male, Age:193 days Diet:Ad lib


title: Spinal Cord, mouse17K-B, 22 AMC27 F 498 C

description: 22 AMC27 F 498 C

value: female, Age:498 days Diet:Caloric restriction


title: RE-Th2-1b

status: Public on Jul 07 2007

hyb_protocol: cDNA probe prepared from each cell type were hybridized with two cDNA filters at 43°C overnight in MicroHyb solution with 1 ng/ml poly dA, 1 mg/ml denatured mouse Cot-1, and 200 mg/ml denatured salmon sperm DNA (InVitroge).  The filters were then washed with SSC (2X) with 1% SDS for 30 minutes at 65°C and SSC (0.5X) with 1% SDS for 30 minutes at 65°C, and exposed to phosphorimaging screen for 2 days.

scan_protocol: Imaging screens were scanned at 50um resolution in a Typhoon 9410 Imager

data_processing: Images were processed by using the Array-Pro Analyzer 4.0 software (Media Cybernetics).  In brief, the spots on the image of the microarray were located, the intensity of the spots were quantitated with subtraction of the background.  The resulting intensity values were normalized filterwide with the median intensity value.  Statistical comparisons between two subsets of cells were conducted by the analysis of variance (ANOVA) with false discovery rate (FDR) modification (Benjamin, J. R. Stat. Soc. Ser. B 57 (1995), pp. 289–300).  The cDNA clones were considered as significantly different between two subsets of cells if they meet three criteria:  1) the p values of FDR > 0.01, 2) the intensity changes > 2.0 fold, and 3) the normalized intensity of clone from the highly expressed cell > 1.0 to ensure the detection.

source_name: Mus musculus thymus

label_protocol: 5 to 15 ug of total RNAs isolated from each cell type using SuperScript II TM RNase H-Reverse Teranscriptase (InVitrogen, Carlsbad, California) in the presence of 100 uCi a-33P-dCTP (PerkinElmer Life Sciences) and 500 ng oligo (dT)12-18 primers (InVitrogen).

growth_protocol: Naïve CD4+ T cells were subjected to 4 days of in-vitro stimulation with antigen presenting cells (APC) under Th2 conditions generating Th2 effector cells. After an additional 3 days culture in the absence of APC, those effector cells become rested expressing a phenotype similar to memory cells (CD44 hi, CD62L lo, CD45RB lo and CD25-).

extract_protocol: total RNA were isolated from cryopreseved CD4+ T cells using STAT60 (TEL-TEST, Inc., Friendwood, TX)

tag: CD4+ Th2 resting effector T cells from spleen of AND TcR transgenic/green fluorescence protein (GFP) transgenic mice


title: E-Th2-2b

growth_protocol: Naïve CD4+ T cells were subjected to 4 days of in-vitro stimulation with antigen presenting cells (APC) under Th2 conditions generating Th2 effector cells.

tag: CD4+ Th2 effector T cells from spleen of AND TcR transgenic/green fluorescence protein (GFP) transgenic mice


title: M-Th2-2b

growth_protocol: Naïve CD4+ T cells were subjected to 4 days of in-vitro stimulation with antigen presenting cells (APC) under Th2 conditions generating Th2 effector cells. After an additional 3 days culture in the absence of APC, those effector cells become rested expressing a phenotype similar to memory cells (CD44 hi, CD62L lo, CD45RB lo and CD25-). These rested effector cells were adaptively transferred into thymectomized, lethally irradiated, and T cell depleted bone marrow reconstituted mice and memory cells were isolated after 4-12 weeks by flow sort.

tag: CD4+ Th2 memory T cells from spleen of AND TcR transgenic/green fluorescence protein (GFP) transgenic mice


title: M-Th2-1b


title: E-Th2-3a


title: RE-Th2-3b


title: RE-Th2-2b


title: RE-Th2-3a

growth_protocol: Naïve CD4+ T cells were subjected to 4 days of in-vitro stimulation with antigen presenting cells (APC) under Th2 conditions generating Th2effector cells. After an additional 3 days culture in the absence of APC, those effector cells become rested expressing a phenotype similar to memory cells (CD44 hi, CD62L lo, CD45RB lo and CD25-).


title: M-Th2-3b


title: E-Th2-3b


title: M-Th2-2a


title: M-Th1-2b

growth_protocol: Naïve CD4+ T cells were subjected to 4 days of in-vitro stimulation with antigen presenting cells (APC) under Th1 conditions generating Th1 effector cells. After an additional 3 days culture in the absence of APC, those effector cells become rested expressing a phenotype similar to memory cells (CD44 hi, CD62L lo, CD45RB lo and CD25-).  These rested effector cells were adaptively transferred into thymectomized, lethally irradiated, and T cell depleted bone marrow reconstituted mice and memory cells were isolated after 4-12 weeks by flow sort.

tag: CD4+ Th1 memory T cells from spleen of AND TcR transgenic/green fluorescence protein (GFP) transgenic mice


title: M-Th2-1a


title: M-Th1-3a


title: E-Th2-1a


title: M-Th2-3a


title: RE-Th2-2a


title: E-Th2-1b


title: RE-Th1-3b

growth_protocol: Naïve CD4+ T cells were subjected to 4 days of in-vitro stimulation with antigen presenting cells (APC) under Th1 conditions generating Th1 effector cells. After an additional 3 days culture in the absence of APC, those effector cells become rested expressing a phenotype similar to memory cells (CD44 hi, CD62L lo, CD45RB lo and CD25-).

tag: CD4+ Th1 resting effector T cells from spleen of AND TcR transgenic/green fluorescence protein (GFP) transgenic mice


title: M-Th1-1a


title: M-Th1-2a


title: M-Th1-3b


title: RE-Th1-3a


title: M-Th1-1b


title: RE-Th1-2b


title: RE-Th1-2a


title: RE-Th1-1b


title: E-Th1-3b

growth_protocol: Naïve CD4+ T cells were subjected to 4 days of in-vitro stimulation with antigen presenting cells (APC) under Th1 conditions generating Th1 effector cells..

tag: CD4+ Th1 effector T cells from spleen of AND TcR transgenic/green fluorescence protein (GFP) transgenic mice


title: RE-Th1-1a


title: E-Th1-2a


title: E-Th1-1b


title: E-Th1-1a


title: N-3b

growth_protocol: Naïve CD4+ T cells were isolated from spleen of AND TcR transgenic/green fluorescence protein (GFP) transgenic mice (Kaye et al., Nature 1989;341:746, Wright et al, Blood 2001;97:2278) that recognize a peptide of pigeon cytochrome C in the context of I-Ek and express CD44lo, CD62Lhi, CD45RBhi, and CD25-.

tag: CD4+ naive T cells from spleen of AND TcR transgenic/green fluorescence protein (GFP) transgenic mice


title: E-Th1-3a


title: N-2a


title: N-3a


title: N-1a


title: N-1b


title: N-2b


title: E-Th1-2b


title: GFP control 2

status: Public on Jul 01 2007

hyb_protocol: Radiolabeled cDNA was denatured at 95 degrees C for 7 minutes and added to 12 mls of Invitrogen microhyb containing 10% dextran sulfate with Cot-1 DNA (Invitrogen) and Poly-A DNA oligos, (Sigma) added as blocking agents.  Arrays were hybridized overnight at 55 degrees C to human MGC cDNA filters.  The hybridized filters were quickly rinsed once and then washed twice at 65 degrees placed onto steel plates covered with plastic wrap and exposed to Kodak storage Phosphorimaging screens for 3-4 days.

organism: Homo sapiens

source_name: Homo sapiens

growth_protocol: SH-SY5Y human neuroblastoma cell were grown in Advanced-Dulbecco's modified Eagle's medium (Gibco life Technologies, Gaithersburg, MD), supplemented with 5% fetal Clone III bovine serum (HyClone, Logan,UT), 2 mM L-glutamine, 100U/ml penicillin G sodium, and 100 ug/ml streptomycin sulfate.  Cell cultures were maintained in 37°C in a humidified 5% CO2/95% air atmosphere.

extract_protocol: Qiagen RNeasy Mini Kit protocol

treatment_protocol: Transfected SH-SY5Y cells were selected in the presence of 500 ug/ml genetecin. On post-transfection day 7, total RNA from GFP vector and DN-TRF2 transfected cells was prepared for transcriptome analyses using the NIA MGC1 9,600 human gene array.  Samples were collected from three independent experiments and were processed in parallel.

tag: Cell line

value: SH-SY5Y from ATCC, Vector:pEGF-C2, cDNA insert:none


title: GFP control 1


title: GFP control 3


title: DN-TRF2-1

treatment_protocol: Transfected SH-SY5Y cells were selected in the presence of 500 ug/ml genetecin. On post-transfection day 7, total RNA from GFP vector and DN-TRF2 transfected cells was prepared for transcriptome analyses using the NIA MGC1 9,600 human gene array.  Samples were collected from three independent experiments and were processed in parallel.  cDNA insertion reference: Science. 1999 Feb 26;283(5406):1321-5.

value: SH-SY5Y from ATCC, Vector:pIRES2-EGFP, cDNA insert:dominant-negative form of TRF2


title: E-Th2-2a


title: DN-TRF2-3


title: DN-TRF2-2


title: RE-Th2-1a


title: Thymus, mouse17K-A, 66 AMC163 M 733_ad-lib

status: Public on May 18 2007

description: 66 AMC163 M 733 A

value: C57BL/6, Tissue:thymus

value: male, Age:733 days Diet:Ad lib


title: Thymus, mouse17K-A, 67 AMC174 F 704_ad-lib

description: 67 AMC174 F 704 A

value: female, Age:704 days Diet:Ad lib


title: Thymus, mouse17K-A, 63 AMC170 M 495_ad-lib

description: 63 AMC170 M 495 A

value: male, Age:495 days Diet:Ad lib


title: Thymus, mouse17K-A, 64 AMC131 F 479_ad-lib

description: 64 AMC131 F 479 A

value: female, Age:479 days Diet:Ad lib


title: Thymus, mouse17K-A, 61 AMC209 M 482 C

description: 61 AMC209 M 482 C

value: male, Age:482 days Diet:Caloric restriction


title: Thymus, mouse17K-A, 60 AMC137 F 180_ad-lib

description: 60 AMC137 F 180 A

value: female, Age:180 days Diet:Ad lib


title: Thymus, mouse17K-A, 65 AMC150 F 703 C

description: 65 AMC150 F 703 C

value: female, Age:703 days Diet:Caloric restriction


title: Thymus, mouse17K-A, 57 AMC244 F 33_ad-lib

description: 57 AMC244 F 33 A

value: female, Age:33 days Diet:Ad lib


title: Thymus, mouse17K-A, 56 AMC239 M 40_ad-lib

description: 56 AMC239 M 40 A

value: male, Age:40 days Diet:Ad lib


title: Thymus, mouse17K-A, 54 AMC119 M 730_ad-lib

description: 54 AMC119 M 730 A

value: male, Age:730 days Diet:Ad lib


title: Thymus, mouse17K-A, 55 AMC167 F 733_ad-lib

description: 55 AMC167 F 733 A

value: female, Age:733 days Diet:Ad lib


title: Thymus, mouse17K-A, 58 AMC158 F 190 C

description: 58 AMC158 F 190 C

value: female, Age:190 days Diet:Caloric restriction


title: Thymus, mouse17K-A, 49 AMC245 M 673 C

description: 49 AMC245 M 673 C

value: male, Age:673 days Diet:Caloric restriction


title: Thymus, mouse17K-A, 50 AMC217 M 177 C

description: 50 AMC217 M 177 C

value: male, Age:177 days Diet:Caloric restriction


title: Thymus, mouse17K-A, 62 AMC152 F 495 C

description: 62 AMC152 F 495 C

value: female, Age:495 days Diet:Caloric restriction


title: Thymus, mouse17K-A, 52 AMC130 F 481_ad-lib

description: 52 AMC130 F 481 A

value: female, Age:481 days Diet:Ad lib


title: Thymus, mouse17K-A, 51 AMC127 M 485_ad-lib

description: 51 AMC127 M 485 A

value: C57BL/6, Tissue:thymus

value: male, Age:485 days Diet:Ad lib


title: Thymus, mouse17K-A, 53 AMC56 F 714 C

description: 53 AMC56 F 714 C

value: female, Age:714 days Diet:Caloric restriction


title: Thymus, mouse17K-A, 48 AMC104 F 485 C

description: 48 AMC104 F 485 C

value: female, Age:485 days Diet:Caloric restriction


title: Thymus, mouse17K-A, 41 AMC74 F 712_ad-lib

description: 41 AMC74 F 712 A

value: female, Age:712 days Diet:Ad lib


title: Thymus, mouse17K-A, 37 AMC125 M 481_ad-lib

description: 37 AMC125 M 481 A

value: male, Age:481 days Diet:Ad lib


title: Thymus, mouse17K-A, 38 AMC86 F 476_ad-lib

description: 38 AMC86 F 476 A

value: female, Age:476 days Diet:Ad lib


title: Thymus, mouse17K-A, 46 AMC92 F 169_ad-lib

description: 46 AMC92 F 169 A

value: female, Age:169 days Diet:Ad lib


title: Thymus, mouse17K-A, 42 AMC194 M 17_ad-lib

description: 42 AMC194 M 17 A

value: male, Age:17 days Diet:Ad lib


title: Thymus, mouse17K-A, 40 AMC118 M 719_ad-lib

description: 40 AMC118 M 719 A

value: male, Age:719 days Diet:Ad lib


title: Thymus, mouse17K-A, 45 AMC133 M 180_ad-lib

description: 45 AMC133 M 180 A

value: male, Age:180 days Diet:Ad lib


title: Thymus, mouse17K-A, 47 AMC148 M 495 C

description: 47 AMC148 M 495 C

value: male, Age:495 days Diet:Caloric restriction


title: Thymus, mouse17K-A, 39 AMC55 F 712 C

description: 39 AMC55 F 712 C

value: female, Age:712 days Diet:Caloric restriction


title: Thymus, mouse17K-A, 44 AMC115 F 174 C

description: 44 AMC115 F 174 C

value: female, Age:174 days Diet:Caloric restriction


title: Thymus, mouse17K-A, 43 AMC241 F 40_ad-lib

description: 43 AMC241 F 40 A

value: female, Age:40 days Diet:Ad lib


title: Thymus, mouse17K-A, 33 AMC51 M 194_ad-lib

description: 33 AMC51 M 194 A

value: male, Age:194 days Diet:Ad lib


title: Thymus, mouse17K-A, 36 AMC28 F 499 C

description: 36 AMC28 F 499 C

value: female, Age:499 days Diet:Caloric restriction


title: Thymus, mouse17K-A, 34 AMC53 F 194_ad-lib

description: 34 AMC53 F 194 A

value: C57BL/6, Tissue:thymus

value: female, Age:194 days Diet:Ad lib


title: Thymus, mouse17K-A, 35 AMC101 M 485 C

description: 35 AMC101 M 485 C

value: male, Age:485 days Diet:Caloric restriction


title: Thymus, mouse17K-A, 31 AMC31 M 194 C

description: 31 AMC31 M 194 C

value: male, Age:194 days Diet:Caloric restriction


title: Thymus, mouse17K-A, 32 AMC113 F 180 C

description: 32 AMC113 F 180 C

value: female, Age:180 days Diet:Caloric restriction


title: Thymus, mouse17K-B, 67 AMC174 F 704_ad-lib

description: 67 AMC174 F 704 A

value: female, Age:704 days Diet:Ad lib


title: Thymus, mouse17K-A, 30 AMC198 F 21_ad-lib

description: 30 AMC198 F 21 A

value: female, Age:21 days Diet:Ad lib


title: Thymus, mouse17K-B, 65 AMC150 F 703 C

description: 65 AMC150 F 703 C

value: female, Age:703 days Diet:Caloric restriction


title: Thymus, mouse17K-B, 66 AMC163 M 733_ad-lib

description: 66 AMC163 M 733 A

value: male, Age:733 days Diet:Ad lib


title: Thymus, mouse17K-B, 64 AMC131 F 479_ad-lib

description: 64 AMC131 F 479 A

value: female, Age:479 days Diet:Ad lib


title: Thymus, mouse17K-A, 29 AMC193 M 19_ad-lib

description: 29 AMC193 M 19 A

value: male, Age:19 days Diet:Ad lib


title: Thymus, mouse17K-A, 24 AMC48 F 499_ad-lib

description: 24 AMC48 F 499 A

value: female, Age:499 days Diet:Ad lib


title: Thymus, mouse17K-A, 25 AMC208 M 673 C

description: 25 AMC208 M 673 C

value: male, Age:673 days Diet:Caloric restriction


title: Thymus, mouse17K-A, 27 AMC76 M 714_ad-lib

description: 01 AMC185 M  43 A

value: male, Age:714 days Diet:Ad lib


title: Thymus, mouse17K-A, 26 AMC25 F 743 C

description: 26 AMC25 F 743 C

value: female, Age:743 days Diet:Caloric restriction


title: Thymus, mouse17K-A, 22 AMC27 F 498 C

description: 22 AMC27 F 498 C

value: female, Age:498 days Diet:Caloric restriction


title: Thymus, mouse17K-B, 63 AMC170 M 495_ad-lib

description: 63 AMC170 M 495 A

value: male, Age:495 days Diet:Ad lib


title: Thymus, mouse17K-B, 61 AMC209 M 482 C

description: 61 AMC209 M 482 C

value: C57BL/6, Tissue:thymus

value: male, Age:482 days Diet:Caloric restriction


title: Thymus, mouse17K-B, 59 AMC135 M 174_ad-lib

description: 59 AMC135 M 174 A

value: male, Age:174 days Diet:Ad lib


title: Thymus, mouse17K-B, 62 AMC152 F 495 C

description: 62 AMC152 F 495 C

value: female, Age:495 days Diet:Caloric restriction


title: Thymus, mouse17K-B, 58 AMC158 F 190 C

description: 58 AMC158 F 190 C

value: female, Age:190 days Diet:Caloric restriction


title: Thymus, mouse17K-B, 60 AMC137 F 180_ad-lib

description: 60 AMC137 F 180 A

value: female, Age:180 days Diet:Ad lib


title: Thymus, mouse17K-A, 20 AMC52 F 193_ad-lib

description: 20 AMC52 F 193 A

value: female, Age:193 days Diet:Ad lib


title: Thymus, mouse17K-A, 21 AMC58 M 472 C

description: 21 AMC58 M 472 C

value: male, Age:472 days Diet:Caloric restriction


title: Thymus, mouse17K-A, 28 AMC73 F 714_ad-lib

description: 28 AMC73 F 714 A

value: female, Age:714 days Diet:Ad lib


title: Thymus, mouse17K-B, 54 AMC119 M 730_ad-lib

description: 54 AMC119 M 730 A

value: male, Age:730 days Diet:Ad lib


title: Thymus, mouse17K-A, 19 AMC50 M 193_ad-lib

description: 19 AMC50 M 193 A

value: male, Age:193 days Diet:Ad lib


title: Thymus, mouse17K-B, 56 AMC239 M 40_ad-lib

description: 56 AMC239 M 40 A

value: male, Age:40 days Diet:Ad lib


title: Thymus, mouse17K-B, 53 AMC56 F 714 C

description: 53 AMC56 F 714 C

value: female, Age:714 days Diet:Caloric restriction


title: Thymus, mouse17K-B, 51 AMC127 M 485_ad-lib

description: 51 AMC127 M 485 A

value: male, Age:485 days Diet:Ad lib


title: Thymus, mouse17K-B, 52 AMC130 F 481_ad-lib

description: 52 AMC130 F 481 A

value: female, Age:481 days Diet:Ad lib


title: Thymus, mouse17K-B, 55 AMC167 F 733_ad-lib

description: 55 AMC167 F 733 A

value: female, Age:733 days Diet:Ad lib


title: Thymus, mouse17K-A, 23 AMC80 M 476_ad-lib

description: 23 AMC80 M 476 A

value: male, Age:476 days Diet:Ad lib


title: Thymus, mouse17K-B, 48 AMC104 F 485 C

description: 48 AMC104 F 485 C

value: C57BL/6, Tissue:thymus

value: female, Age:485 days Diet:Caloric restriction


title: Thymus, mouse17K-B, 49 AMC245 M 673 C

description: 49 AMC245 M 673 C

value: male, Age:673 days Diet:Caloric restriction


title: Thymus, mouse17K-B, 50 AMC217 M 177 C

description: 50 AMC217 M 177 C

value: male, Age:177 days Diet:Caloric restriction


title: Thymus, mouse17K-A, 18 AMC34 F 194 C

description: 18 AMC34 F 194 C

value: female, Age:194 days Diet:Caloric restriction


title: Thymus, mouse17K-B, 47 AMC148 M 495 C

description: 47 AMC148 M 495 C

value: male, Age:495 days Diet:Caloric restriction


title: Thymus, mouse17K-A, 15 AMC186 M 44_ad-lib

description: 15 AMC186 M 44 A

value: male, Age:44 days Diet:Ad lib


title: Thymus, mouse17K-A, 16 AMC197 F 19_ad-lib

description: 16 AMC197 F 19 A

value: female, Age:19 days Diet:Ad lib


title: Thymus, mouse17K-B, 43 AMC241 F 40_ad-lib

description: 43 AMC241 F 40 A

value: female, Age:40 days Diet:Ad lib


title: Thymus, mouse17K-B, 40 AMC118 M 719_ad-lib

description: 40 AMC118 M 719 A

value: male, Age:719 days Diet:Ad lib


title: Thymus, mouse17K-A, 11 AMC207 M 673 C

description: 11 AMC207 M 673 C


title: Thymus, mouse17K-A, 12 AMC1 F 736 C

description: 12 AMC1 F 736 C

value: female, Age:736 days Diet:Caloric restriction


title: Thymus, mouse17K-B, 45 AMC133 M 180_ad-lib

description: 45 AMC133 M 180 A

value: male, Age:180 days Diet:Ad lib


title: Thymus, mouse17K-B, 44 AMC115 F 174 C

description: 44 AMC115 F 174 C

value: female, Age:174 days Diet:Caloric restriction


title: Thymus, mouse17K-A, 17 AMC30 M 193 C

description: 17 AMC30 M 193 C

value: male, Age:193 days Diet:Caloric restriction


title: Thymus, mouse17K-B, 42 AMC194 M 17_ad-lib

description: 42 AMC194 M 17 A

value: male, Age:17 days Diet:Ad lib


title: Thymus, mouse17K-A, 13 AMC36 M 743_ad-lib

description: 13 AMC36 M 743 A

value: male, Age:743 days Diet:Ad lib


title: Thymus, mouse17K-B, 41 AMC74 F 712_ad-lib

description: 41 AMC74 F 712 A

value: female, Age:712 days Diet:Ad lib


title: Thymus, mouse17K-A, 14 AMC72 F 716_ad-lib

description: 14 AMC72 F 716 A

value: C57BL/6, Tissue:thymus

value: female, Age:716 days Diet:Ad lib


title: Thymus, mouse17K-A, 10 AMC46 F 498_ad-lib

description: 10 AMC46 F 498 A

value: female, Age:498 days Diet:Ad lib


title: Thymus, mouse17K-B, 39 AMC55 F 712 C

description: 39 AMC55 F 712 C

value: female, Age:712 days Diet:Caloric restriction


title: Thymus, mouse17K-B, 37 AMC125 M 481_ad-lib

description: 37 AMC125 M 481 A

value: male, Age:481 days Diet:Ad lib


title: Thymus, mouse17K-B, 46 AMC92 F 169_ad-lib

description: 46 AMC92 F 169 A

value: female, Age:169 days Diet:Ad lib


title: Thymus, mouse17K-B, 57 AMC244 F 33_ad-lib

description: 57 AMC244 F 33 A

value: female, Age:33 days Diet:Ad lib


title: Thymus, mouse17K-B, 38 AMC86 F 476_ad-lib

description: 38 AMC86 F 476 A

value: female, Age:476 days Diet:Ad lib


title: Thymus, mouse17K-B, 36 AMC28 F 499 C

description: 36 AMC28 F 499 C

value: female, Age:499 days Diet:Caloric restriction


title: Thymus, mouse17K-B, 34 AMC53 F 194_ad-lib

description: 34 AMC53 F 194 A

value: female, Age:194 days Diet:Ad lib


title: Thymus, mouse17K-B, 35 AMC101 M 485 C

description: 35 AMC101 M 485 C

value: male, Age:485 days Diet:Caloric restriction


title: Thymus, mouse17K-A, 09 AMC43 M 498_ad-lib

description: 09 AMC43 M 498 A

value: male, Age:498 days Diet:Ad lib


title: Thymus, mouse17K-A, 08 AMC4 F 491 C

description: 08 AMC4 F 491 C

value: female, Age:491 days Diet:Caloric restriction


title: Thymus, mouse17K-A, 06 AMC23 F 186_ad-lib

description: 06 AMC23 F 186 A

value: female, Age:186 days Diet:Ad lib


title: Thymus, mouse17K-A, 05 AMC21 M 186_ad-lib

description: 05 AMC21 M 186 A

value: male, Age:186 days Diet:Ad lib


title: Thymus, mouse17K-B, 28 AMC73 F 714_ad-lib

description: 28 AMC73 F 714 A

value: female, Age:714 days Diet:Ad lib


title: Thymus, mouse17K-A, 04 AMC33 F 193 C

description: 04 AMC33 F 193 C

value: female, Age:193 days Diet:Caloric restriction


title: Thymus, mouse17K-B, 23 AMC80 M 476_ad-lib

description: 23 AMC80 M 476 A

value: C57BL/6, Tissue:thymus

value: male, Age:476 days Diet:Ad lib


title: Thymus, mouse17K-B, 22 AMC27 F 498 C

description: 22 AMC27 F 498 C

value: female, Age:498 days Diet:Caloric restriction


title: Thymus, mouse17K-B, 24 AMC48 F 499_ad-lib

description: 24 AMC48 F 499 A

value: female, Age:499 days Diet:Ad lib


title: Thymus, mouse17K-A, 03 AMC7 M 186 C

description: 03 AMC7 M 186 C

value: male, Age:186 days Diet:Caloric restriction


title: Thymus, mouse17K-B, 20 AMC52 F 193_ad-lib

description: 20 AMC52 F 193 A

value: female, Age:193 days Diet:Ad lib


title: Thymus, mouse17K-B, 18 AMC34 F 194 C

description: 18 AMC34 F 194 C

value: female, Age:194 days Diet:Caloric restriction


title: Thymus, mouse17K-B, 19 AMC50 M 193_ad-lib

description: 19 AMC50 M 193 A

value: male, Age:193 days Diet:Ad lib


title: Thymus, mouse17K-B, 31 AMC31 M 194 C

description: 31 AMC31 M 194 C

value: male, Age:194 days Diet:Caloric restriction


title: Thymus, mouse17K-B, 21 AMC58 M 472 C

description: 21 AMC58 M 472 C

value: male, Age:472 days Diet:Caloric restriction


title: Thymus, mouse17K-A, 07 AMC57 M 474 C

description: 07 AMC57 M 474 C

value: male, Age:474 days Diet:Caloric restriction


title: Thymus, mouse17K-B, 17 AMC30 M 193 C

description: 17 AMC30 M 193 C

value: male, Age:193 days Diet:Caloric restriction


title: Thymus, mouse17K-B, 29 AMC193 M 19_ad-lib

description: 29 AMC193 M 19 A

value: male, Age:19 days Diet:Ad lib


title: Thymus, mouse17K-A, 02 AMC196 F 17_ad-lib

description: 02 AMC196 F 17 A

value: female, Age:17 days Diet:Ad lib


title: Thymus, mouse17K-B, 16 AMC197 F 19_ad-lib

description: 16 AMC197 F 19 A

value: female, Age:19 days Diet:Ad lib


title: Thymus, mouse17K-A, 01 AMC185 M 43_ad-lib

description: 01 AMC185 M 43 A

value: male, Age:43 days Diet:Ad lib


title: Thymus, mouse17K-B, 33 AMC51 M 194_ad-lib

description: 33 AMC51 M 194 A

value: male, Age:194 days Diet:Ad lib


title: Thymus, mouse17K-B, 30 AMC198 F 21_ad-lib

description: 30 AMC198 F 21 A

value: C57BL/6, Tissue:thymus

value: female, Age:21 days Diet:Ad lib


title: Thymus, mouse17K-B, 25 AMC208 M 673 C

description: 25 AMC208 M 673 C

value: male, Age:673 days Diet:Caloric restriction


title: Thymus, mouse17K-B, 26 AMC25 F 743 C

description: 26 AMC25 F 743 C

value: female, Age:743 days Diet:Caloric restriction


title: Thymus, mouse17K-B, 27 AMC76 M 714_ad-lib

description: 01 AMC185 M  43 A

value: male, Age:714 days Diet:Ad lib


title: Thymus, mouse17K-B, 14 AMC72 F 716_ad-lib

description: 14 AMC72 F 716 A

value: female, Age:716 days Diet:Ad lib


title: Thymus, mouse17K-B, 32 AMC113 F 180 C

description: 32 AMC113 F 180 C

value: female, Age:180 days Diet:Caloric restriction


title: Thymus, mouse17K-B, 13 AMC36 M 743_ad-lib

description: 13 AMC36 M 743 A

value: male, Age:743 days Diet:Ad lib


title: Thymus, mouse17K-B, 12 AMC1 F 736 C

description: 12 AMC1 F 736 C

value: female, Age:736 days Diet:Caloric restriction


title: Thymus, mouse17K-B, 11 AMC207 M 673 C

description: 11 AMC207 M 673 C


title: Thymus, mouse17K-B, 10 AMC46 F 498_ad-lib

description: 10 AMC46 F 498 A

value: female, Age:498 days Diet:Ad lib


title: Thymus, mouse17K-B, 08 AMC4 F 491 C

description: 08 AMC4 F 491 C

value: female, Age:491 days Diet:Caloric restriction


title: Thymus, mouse17K-B, 04 AMC33 F 193 C

description: 04 AMC33 F 193 C

value: female, Age:193 days Diet:Caloric restriction


title: Thymus, mouse17K-B, 09 AMC43 M 498_ad-lib

description: 09 AMC43 M 498 A

value: male, Age:498 days Diet:Ad lib


title: Thymus, mouse17K-B, 07 AMC57 M 474 C

description: 07 AMC57 M 474 C

value: male, Age:474 days Diet:Caloric restriction


title: Hippocampus, mouse17K-A, 30 AMC198 F 21_ad-lib

description: 30 AMC198 F 21 A

source_name: Mus musculus Hippocampus

value: C57BL/6, Tissue:Hippocampus


title: Thymus, mouse17K-B, 06 AMC23 F 186_ad-lib

description: 06 AMC23 F 186 A

value: female, Age:186 days Diet:Ad lib


title: Thymus, mouse17K-B, 05 AMC21 M 186_ad-lib

description: 05 AMC21 M 186 A

value: male, Age:186 days Diet:Ad lib


title: Thymus, mouse17K-B, 03 AMC7 M 186 C

description: 03 AMC7 M 186 C

value: C57BL/6, Tissue:thymus

value: male, Age:186 days Diet:Caloric restriction


title: Thymus, mouse17K-B, 02 AMC196 F 17_ad-lib

description: 02 AMC196 F 17 A

value: female, Age:17 days Diet:Ad lib


title: Thymus, mouse17K-A, 59 AMC135 M 174_ad-lib

description: 59 AMC135 M 174 A

value: male, Age:174 days Diet:Ad lib


title: Thymus, mouse17K-B, 01 AMC185 M 43_ad-lib

description: 01 AMC185 M 43 A

value: male, Age:43 days Diet:Ad lib


title: liver High Calorie Diet 2

status: Public on Nov 03 2006

description: liver  Control High Calorie Diet 2

hyb_protocol: 17 hour incubation following Agilent protocols using 750ng of both Cy3 and Cy5 labeled material, In Situ Hybridization Kit reagents and control probes followed by a 10 minute room temperature wash in 6X SSC,0.005% tritonX-102 and 5 minutes at 4 degrees C in 0.1X SSC, 0.005% Triton X-102. The arrays were dried in a Nitrogen stream and scanned.

scan_protocol: Arrays were scanned using an Agilent Scanner G2565AA .

data_processing:
Data from both channels was extracted using the Agilent Feature Extractor Software, Version 7.5.  Due to potential variation of the Cy-5 dye we prefer to analyze the green channel alone and use single channel data.  Results for control probes were first removed, multiple repeats of any probe were averaged, then each array was z-normalized.  Differently expressed genes were identified between the 3 sample groups and pathway enrichment analysis was conducted using Diane 6.0, an in-house microarray analysis program.

Both the green channel and red channel raw data are available in the Supplementary file at the foot of the Sample record.

source_name: High Calorie, Ad Lib, Liver

label_protocol: Agilent low RNA Input Linear Amplification Kit using Cyanine-3-CTP

treatment_protocol: Mice were fed AIN-93G modified to provide 60% of calories from fat, Ad Lib

tag: C57BL/6NIA Mice, 18months of age, High Calorie, Ad Lib, Liver

source_name: Standard Diet, Ad Lib, Liver, Pools of 5 samples

label_protocol: Agilent low RNA Input Linear Amplification Kit using Cyanine-5-CTP

extract_protocol: Total RNA was extracted with the Qiagen RNEasy Columns and 5 samples were pooled

treatment_protocol: Mice were fed AIN-93G Standard Diet, Ad Lib

tag: C57BL/6NIA Mice, 18months of age, Standard Diet, Ad Lib, Liver pools


title: liver Control Standard Diet 4

description: liver  Control Standard Diet 4

source_name: Normal Diet, Ad Lib, Liver

extract_protocol: Total RNA was extracted with the Qiagen RNEasy Columns

tag: C57BL/6NIA Mice, 18months of age, Standard Diet, Ad Lib, Liver


title: liver Control Standard Diet 3

description: liver  Control Standard Diet 3


title: liver High Calorie Diet 1

description: liver  Control High Calorie Diet 1

source_name: High Calorie Ad Lib, Liver


title: liver High Calorie Diet 3

description: liver  Control High Calorie Diet 3


title: liver Control Standard Diet 5

description: liver  Control Standard Diet 5


title: liver Control Standard Diet 2

description: liver  Control Standard Diet 2


title: liver High Calorie Plus Resveratrol Diet 4

description: liver  Control High Calorie Plus Resveratrol Diet 4

source_name: High Calorie, 0.04% Resveratrol, Ad Lib, Liver

treatment_protocol: Mice were fed AIN-93G modified to provide 60% of calories from fat, plus 0.04% resveratrol, Ad Lib

tag: C57BL/6NIA Mice, 18months of age, High Calorie, Plus Resveratrol, Ad Lib, Liver


title: liver High Calorie Diet 5

description: liver  Control High Calorie Diet 5


title: liver High Calorie Plus Resveratrol Diet 3

description: liver  Control High Calorie Plus Resveratrol Diet 3


title: liver High Calorie Diet 4

description: liver  Control High Calorie Diet 4


title: liver High Calorie Plus Resveratrol Diet 1

description: liver  Control High Calorie Plus Resveratrol Diet 1


title: liver Control Standard Diet 1

description: liver  Control Standard Diet 1


title: liver High Calorie Plus Resveratrol Diet 2

description: liver  Control High Calorie Plus Resveratrol Diet 2


title: AgeMapMouse#194 M 17 A - array b

status: Public on Nov 24 2004

description:
AgeMapMouse#194, Male, sacrificed at 17 days (1st age group) diet ad libidum.
Keywords = Liver
Keywords = caloric restriction
Keywords = aging

source_name: Liver tissue


title: AgeMapMouse#217 M 177 C - array B

description:
AgeMapMouse#217, Male, sacrificed at 177 days (2nd age group) diet caloric restriction.
Keywords = Liver
Keywords = caloric restriction
Keywords = aging


title: AgeMapMouse#135 M 174 A - array B

description:
AgeMapMouse#135, Male, sacrificed at 174 days (2nd age group) diet ad libidum.
Keywords = Liver
Keywords = caloric restriction
Keywords = aging

source_name: liver tissue


title: AgeMapMouse#135 M 174 A - array A


title: AgeMapMouse#194 M 17 A - array A


title: AgeMapMouse#217 M 177 C - array A


title: Thymus, mouse17K-B, 15 AMC186 M 44_ad-lib

description: 15 AMC186 M 44 A

value: male, Age:44 days Diet:Ad lib