title: 46188: M12_Nkx_Hi_patUPD

value: Nkx2.1 lineage

value: Hippocampus

value: pat


title: 46189: M12_Nkx_Hi_matUPD

status: Public on Jul 16 2020

type: SRA

city: Klosterneuburg

first: Florian

middle: M

last: Pauler

address: Am Campus 1

country: Austria

institute: Institute of Science and Technology Austria (IST Austria)

library_source: transcriptomic

data_processing:
Raw reads were delivered as BAM files and converted to fastq format using bamToFastq (bedtools suite v2.26.0) for alignment. Reads were aligned to mm10 and gencode vM16 annotation using STAR  with  --clip5pNbases 12 --outFilterMultimapNmax 1 --outSAMmode BAM --quantMode TranscriptomeSAM GeneCounts. The resulting BAM file was used to count unique reads aligning to the trascriptome, and the number of “raw” reads resulting in 10 million reads aligning to the transcriptome was determined. This number of reads was randomly extracted from the raw reads using DownsampleSam from the picard toolkit (v.2.16.0). These reads were used to count reads per gene, using STAR with --outFilterMultimapNmax 1 --outSAMtype None --outSAMmode None --quantMode GeneCounts --clip5pNbases 12 parameters.
Differential expression between maternal and paternal UPD cells in all cell types was calculated using DESeq2 v1.16.1. Results are given as supplementary file.
Genome_build: mm10/GRCm38
Supplementary_files_format_and_content: Read counts in transcripts as reported by STAR as tab delimited text
Supplementary_files_format_and_content: Excel spreadsheet with differential expression analysis between matUPD and patUPD sample as reported by DESeq2. Cell type given in column headers.

molecule: total RNA

organism: Mus musculus

growth_protocol: All mouse colonies were maintained in accordance with protocols approved by institutional animal care and use committee, institutional ethics committee and the preclinical core facility (PCF) at IST Austria. Experiments were performed under a license approved by the Austrian Federal Ministry of Science and Research in accordance with the Austrian and EU animal laws. Mouse lines used: MADM-7-GT (JAX stock # 021457), MADM-7-TG (JAX stock # 021458), MADM-11-GT (JAX stock # 013749), MADM-11-TG (JAX stock # 013751), MADM-12-GT (JAX stock # 021460), MADM-12-TG (JAX stock # 021461), Emx1-Cre (JAX stock # 005628), Nkx2.1-Cre (JAX stock # 008661)

extract_protocol:
Samples in isolation Buffer (IB; Lexogen) were processed according to the SPLIT kit instructions (Lexogen). All other samples were incubated for 30min at 37°C. Total volume was filled to 250µl using RNase-free H2O (Thermo Fisher Scientific) followed by addition of 750µl Trizol LS (Thermo Fisher Scientific). Samples were mixed by 5 times inverting. After a 5min incubation step at RT, the entire solution was transferred into a MaXtract tube (Qiagen). 200µl chloroform (Sigma-Aldrich) was added, followed by 3 times 5sec vortexing and 2min incubation at RT. Samples were centrifuged for 2min with 12000rpm at 18°C. Supernatant was transferred to a new tube and isopropanol (Sigma-Aldrich) was added in a 1:1 ratio. For better visibility of the RNA pellet 1µl GlycoBlue (Thermo Fisher Scientific) was added and entire solution was mixed by vortexing (3x 5sec). Samples were left for precipitation o/n at -20°C. After precipitation samples were centrifuged for 20min with 14000rpm at 4°C. Supernatant was removed and RNA pellet was washed with 70% ethanol, followed by a 5min centrifugation step (14000rpm at 4°C). RNA pellet was resuspended in an appropriate amount of RNase-free H2O. RNA quality was analyzed using Bioanalyzer RNA 6000 Nano (Agilent) and RNA 6000 Pico kit (Agilent) following the manufacturer’s instructions.
cDNA libraries were prepared using QuantSeq 3’mRNA library preparation kit (Lexogen) following the manufacturer’s instructions. In order to amplify libraries correctly RT-PCR was performed as indicated in the protocol using PCR Add-on kit (Lexogen). cDNA library size distribution was analyzed using Bioanalyzer DNA High Sensitivity kit (Agilent) according to the manufacturer’s instructions. cDNA library concentration was measured using Qubit. Libraries were pooled according to library length and concentration.

treatment_protocol: P0 animals were sacrificed and brain areas of interest (neocortex, hippocampus and olfactory bulb) were dissected. Single cell suspensions were prepared by using Papain containing L-cysteine and EDTA (vial 2, Worthington), DNase I (vial 3, Worthington), Ovomucoid protease inhibitor (vial 4, Worthington), EBSS (Thermo Fisher Scientific), DMEM/F12 (Thermo Fisher Scientific), FBS and HS. All vials from Worthington kit were reconstituted according to the manufacturer’s instructions using EBSS. The dissected brain areas were directly placed into Papain-DNase solution (20units/ml papain and 1000 units DNase). Entire litters were pooled in order to obtain sufficient material for RNA extraction. Enzymatic digestion was performed for 30min at 37°C in a shaking water bath. Next, solution 2 (EBSS containing 0.67mg Ovomucoid protease inhibitor and 166.7 U/ml DNase I) was added, the whole suspension was thoroughly mixed and centrifuged for 5min at 1000rpm at RT. Supernatant was removed and cell pellet was resuspended in solution 2. Trituration with p1000 pipette was performed to mechanically dissolve any remaining tissue parts. DMEM/F12 was added to the cell suspension as a washing solution, followed by a centrifugation step of 5min with 1500rpm at RT. Cells were resuspended in media (DMEM/F12 containing 10% FBS and 10% HS) and kept on ice until sorted. Right before sorting, cell suspension was filtered using a 40µm cell strainer. FACS was performed on a BD FACS Aria III using 100 nozzle and keeping sample and collection devices at 4°C. Duplet exclusion was performed to ensure sorting of true single cells. GFP+ (green), tdTomato+ (red), and GFP+/tdT+ (yellow) cells were collected. Cells were sorted directly into either Isolation Buffer (IB; Lexogen) if more than 2000 sorted cells/per color were expected or if samples yielded fewer labeled cells into custom made lysis buffer (30nM TRIS pH 8, 10nM EDTA pH 8, 1% SDS and 200 µg/µL Proteinase K).

tag: cell type

tag: tissue

tag: madm insertion

tag: strain

tag: color

value: green

tag: upd

value: mat

tag: age


title: 46187: M12_Nkx_Hi_wt

value: yellow

value: wt


title: 46190: M12_Nkx_Hi_wt


title: 46185: M12_Nkx_Hi_matUPD

value: red


title: 46186: M12_Nkx_Hi_patUPD


title: Ruvseq_Patient 746 CBCS_N746

type: RNA

city: Houston

first: Arjun

last: Bhattacharya

state: TX

address: 1155 Pressler Street

department: Epidemiology

country: USA

institute: University of Texas MD Anderson Cancer Center

hyb_protocol: 3 uL of Reporter CodeSet, 5 uL of hybridization buffer, up to 5 uL (25-100 ng) of sample RNA, and 2 uL of Capture ProbeSet was combined for the hybridization reaction. The reactions were incubated at 65 degrees C for at least 16 hours and at most 48 hours and temperature was ramped down to 4 degrees C for processing the following day. Hybridization was run on Prep Station Serial # 0910B0040, Prep Station Serial # 1602D0412. Details provided: https://www.nanostring.com/download_file/view/1808/3822

scan_protocol: NanoString cartridges were scanned using the nCounter MAX Analysis System (NanoString Digital Analyzer Serial # 1605C0377)

data_processing: The raw data were scaled by upper quantile normalization (Bullard et al., 2010, PMID 20167110). One dimension of unwanted technical variation was estimated using Remove Unwanted Variation (Gagnon-Bartsch et al., 2012, PMID 22101192) and removed from the variance-stabilized expression matrix using the limma package (Ritche et al., 2015, PMID 25605792).

organism: Homo sapiens

source_name: primary breast tumor

label_protocol: NanoString nCounter technology

growth_protocol: Samples were recruited by the Carolina Breast Cancer Study, a population-based cohort of breast cancer patients from North Carolina, up-sampled for African American women (Newman et al., 1995, PMID 7612904).

extract_protocol: A study pathologist reviewed hemtoxylin and eosin for each tumor, selected a representative tumor block, and selected tumor areas for coring. Ten 10 uM sections were cut from blocks after coring, and an additional section was obtained and reviewed for tumor cellularity (Troester et al., 2018, PMID 28859290). RNA was isolated from cores using Qiagen RNeasy formalin-fixed, paraffin-embedded kit and protocol (Troester et al., 2018, PMID 28859290).

treatment_protocol: Blood samples, medical record documentation, and paraffin-embedded tumor specimens are obtained for all breast cancer patients (Newman et al., 1995, PMID 7612904).

value: primary breast tumor

tag: study phase

tag: estrogen receptor status

value: Positive

tag: pam50 molecular subtype

value: LumA


title: Ruvseq_Patient 742 CBCS_N742


title: Ruvseq_Patient 741 CBCS_N741


title: Ruvseq_Patient 745 CBCS_N745


title: Ruvseq_Patient 743 CBCS_N743

value: Basal


title: Ruvseq_Patient 756 CBCS_N756


title: 43790: M11_Emx_Hi_patUPD

value: Emx1 lineage


title: Ruvseq_Patient 737 CBCS_N737


title: Ruvseq_Patient 738 CBCS_N738


title: Ruvseq_Patient 736 CBCS_N736

value: LumB


title: 43788: M11_Emx_Hi_matUPD


title: 43787: M11_Emx_Hi_patUPD


title: 43789: M11_Emx_Hi_wt


title: Ruvseq_Patient 735 CBCS_N735


title: Ruvseq_Patient 733 CBCS_N733


title: 43786: M11_Emx_Hi_wt


title: 43792: M11_Emx_Hi_wt


title: Ruvseq_Patient 740 CBCS_N740


title: 43785: M11_Emx_Hi_patUPD


title: Ruvseq_Patient 739 CBCS_N739


title: 43791: M11_Emx_Hi_matUPD


title: 43784: M11_Emx_Hi_matUPD


title: Ruvseq_Patient 732 CBCS_N732


title: Ruvseq_Patient 734 CBCS_N734


title: 43782: M11_Emx_Hi_patUPD


title: 43781: M11_Emx_Hi_matUPD


title: 43783: M11_Emx_Hi_wt


title: Ruvseq_Patient 730 CBCS_N730


title: 42833: M11_Emx_OB_wt

value: Olfactory bulb


title: Ruvseq_Patient 729 CBCS_N729

value: Normal


title: Ruvseq_Patient 731 CBCS_N731


title: Ruvseq_Patient 726 CBCS_N726


title: Ruvseq_Patient 728 CBCS_N728


title: 42831: M11_Emx_OB_patUPD


title: Ruvseq_Patient 727 CBCS_N727


title: 42832: M11_Emx_OB_matUPD


title: Ruvseq_Patient 725 CBCS_N725


title: Ruvseq_Patient 761 CBCS_N761


title: 42830: M11_Emx_OB_wt


title: 42829: M11_Emx_OB_matUPD


title: Ruvseq_Patient 723 CBCS_N723


title: 42828: M11_Emx_OB_patUPD


title: Ruvseq_Patient 722 CBCS_N722


title: Ruvseq_Patient 724 CBCS_N724


title: Ruvseq_Patient 721 CBCS_N721


title: 42827: M11_Emx_OB_wt


title: Ruvseq_Patient 744 CBCS_N744


title: 42826: M11_Emx_OB_patUPD


title: Ruvseq_Patient 720 CBCS_N720


title: 42825: M11_Emx_OB_matUPD


title: 42824: M11_Emx_OB_wt


title: Ruvseq_Patient 719 CBCS_N719


title: 42823: M11_Emx_OB_patUPD


title: 42820: M7_Emx_OB_matUPD


title: 42822: M11_Emx_OB_matUPD


title: 42821: M7_Emx_OB_wt


title: Ruvseq_Patient 718 CBCS_N718


title: Ruvseq_Patient 716 CBCS_N716


title: 42819: M7_Emx_OB_patUPD


title: Ruvseq_Patient 715 CBCS_N715


title: Ruvseq_Patient 714 CBCS_N714


title: 42818: M7_Emx_OB_wt


title: 42816: M7_Emx_OB_patUPD


title: Ruvseq_Patient 712 CBCS_N712


title: 42817: M7_Emx_OB_matUPD


title: Ruvseq_Patient 713 CBCS_N713


title: Ruvseq_Patient 711 CBCS_N711


title: Ruvseq_Patient 710 CBCS_N710


title: 42814: M7_Emx_OB_patUPD


title: Ruvseq_Patient 709 CBCS_N709


title: Ruvseq_Patient 708 CBCS_N708


title: Ruvseq_Patient 707 CBCS_N707


title: Ruvseq_Patient 706 CBCS_N706


title: Ruvseq_Patient 705 CBCS_N705


title: Ruvseq_Patient 717 CBCS_N717


title: 42811: M7_Emx_OB_patUPD


title: 37789: M12_Nkx_Cx_wt

value: Neocortex


title: 42810: M7_Emx_OB_matUPD


title: Ruvseq_Patient 704 CBCS_N704


title: Ruvseq_Patient 703 CBCS_N703


title: Ruvseq_Patient 702 CBCS_N702


title: 37787: M12_Nkx_Cx_patUPD

value: Nkx2.1 lineage


title: 42815: M7_Emx_OB_wt


title: Ruvseq_Patient 701 CBCS_N701


title: 37785: M12_Nkx_Cx_matUPD


title: Ruvseq_Patient 700 CBCS_N700


title: 37788: M12_Nkx_Cx_matUPD


title: 37786: M12_Nkx_Cx_wt


title: 37783: M12_Nkx_Cx_wt


title: Ruvseq_Patient 699 CBCS_N699


title: 37784: M12_Nkx_Cx_patUPD


title: 42813: M7_Emx_OB_matUPD


title: 42812: M7_Emx_OB_wt


title: 37782: M12_Nkx_Cx_patUPD


title: Ruvseq_Patient 697 CBCS_N697


title: Ruvseq_Patient 698 CBCS_N698


title: 37781: M12_Nkx_Cx_matUPD


title: Ruvseq_Patient 695 CBCS_N695


title: Ruvseq_Patient 696 CBCS_N696


title: Ruvseq_Patient 694 CBCS_N694


title: 37779: M12_Nkx_Cx_patUPD


title: Ruvseq_Patient 693 CBCS_N693


title: 37778: M12_Nkx_Cx_matUPD


title: 37776: M12_Emx_Hi_matUPD

value: Hippocampus


title: Ruvseq_Patient 691 CBCS_N691


title: 37775: M12_Emx_Hi_patUPD

value: pat


title: Ruvseq_Patient 690 CBCS_N690


title: Ruvseq_Patient 689 CBCS_N689


title: 37773: M12_Emx_Hi_matUPD

value: green


title: 37774: M12_Emx_Hi_wt


title: Ruvseq_Patient 688 CBCS_N688


title: Ruvseq_Patient 685 CBCS_N685


title: Ruvseq_Patient 686 CBCS_N686


title: 37780: M12_Nkx_Cx_wt


title: 37772: M12_Emx_Hi_patUPD


title: 37771: M12_Emx_Hi_wt


title: Ruvseq_Patient 684 CBCS_N684


title: 37741: M12_Emx_Hi_matUPD

value: mat


title: Ruvseq_Patient 683 CBCS_N683


title: Ruvseq_Patient 681 CBCS_N681


title: 37740: M12_Emx_Hi_wt

value: yellow


title: 37739: M12_Emx_Hi_patUPD


title: 37737: M12_Emx_Cx_wt

value: wt


title: 37777: M12_Emx_Hi_wt


title: 37738: M12_Emx_Hi_matUPD

value: red


title: Ruvseq_Patient 682 CBCS_N682

value: primary breast tumor


title: Ruvseq_Patient 678 CBCS_N678

value: Negative


title: 37736: M12_Emx_Cx_matUPD


title: Ruvseq_Patient 680 CBCS_N680

value: Positive


title: 37735: M12_Emx_Cx_patUPD


title: 37732: M12_Emx_Cx_patUPD


title: 37734: M12_Emx_Cx_wt


title: 37733: M12_Emx_Cx_matUPD


title: Ruvseq_Patient 676 CBCS_N676


title: Ruvseq_Patient 674 CBCS_N674

value: Basal


title: 37731: M12_Emx_Cx_wt

value: Emx1 lineage


title: 37730: M12_Emx_Cx_patUPD


title: Ruvseq_Patient 675 CBCS_N675


title: 37729: M12_Emx_Cx_matUPD


title: Ruvseq_Patient 672 CBCS_N672


title: Ruvseq_Patient 679 CBCS_N679


title: Ruvseq_Patient 671 CBCS_N671


title: 37728: M12_Emx_Cx_wt


title: Ruvseq_Patient 669 CBCS_N669


title: 37727: M12_Emx_Cx_patUPD


title: Ruvseq_Patient 687 CBCS_N687

value: LumB


title: 37726: M12_Emx_Cx_matUPD


title: 35015: M7_Nkx_Hi_wt


title: Ruvseq_Patient 670 CBCS_N670


title: Ruvseq_Patient 667 CBCS_N667


title: Ruvseq_Patient 677 CBCS_N677


title: Ruvseq_Patient 666 CBCS_N666


title: 35013: M7_Nkx_Hi_matUPD


title: Ruvseq_Patient 665 CBCS_N665


title: 35011: M7_Nkx_Cx_wt


title: 35010: M7_Nkx_Cx_patUPD


title: Ruvseq_Patient 664 CBCS_N664


title: 35014: M7_Nkx_Hi_patUPD


title: Ruvseq_Patient 663 CBCS_N663


title: Ruvseq_Patient 662 CBCS_N662


title: Ruvseq_Patient 668 CBCS_N668


title: 35006: M7_Nkx_Hi_matUPD


title: Ruvseq_Patient 660 CBCS_N660


title: Ruvseq_Patient 661 CBCS_N661


title: 35005: M7_Nkx_Hi_patUPD


title: 35009: M7_Nkx_Cx_matUPD


title: 35003: M7_Nkx_Hi_wt


title: 35002: M7_Nkx_Hi_patUPD


title: 35001: M7_Nkx_Hi_matUPD


title: Ruvseq_Patient 659 CBCS_N659


title: Ruvseq_Patient 656 CBCS_N656


title: 34999: M7_Nkx_Cx_wt


title: Ruvseq_Patient 655 CBCS_N655


title: 34997: M7_Nkx_Cx_matUPD


title: Ruvseq_Patient 654 CBCS_N654


title: 35007: M7_Nkx_Hi_wt


title: 34995: M7_Nkx_Hi_wt


title: Ruvseq_Patient 653 CBCS_N653


title: Ruvseq_Patient 652 CBCS_N652


title: 34994: M7_Nkx_Hi_matUPD


title: 34998: M7_Nkx_Cx_patUPD


title: Ruvseq_Patient 651 CBCS_N651


title: 34993: M7_Nkx_Hi_patUPD


title: Ruvseq_Patient 657 CBCS_N657


title: 34991: M7_Nkx_Cx_wt


title: 34990: M7_Nkx_Cx_matUPD


title: Ruvseq_Patient 649 CBCS_N649

value: LumA


title: 33044: M7_Emx_Hi_wt


title: Ruvseq_Patient 648 CBCS_N648


title: Ruvseq_Patient 647 CBCS_N647


title: Ruvseq_Patient 650 CBCS_N650


title: 33043: M7_Emx_Hi_matUPD


title: Ruvseq_Patient 645 CBCS_N645


title: 33042: M7_Emx_Hi_patUPD


title: Ruvseq_Patient 646 CBCS_N646


title: Ruvseq_Patient 644 CBCS_N644

value: Normal


title: 33039: M7_Emx_Hi_matUPD


title: Ruvseq_Patient 643 CBCS_N643


title: 33035: M7_Emx_Hi_patUPD


title: 33036: M7_Emx_Hi_wt


title: Ruvseq_Patient 640 CBCS_N640


title: 33032: M7_Emx_Hi_wt


title: Ruvseq_Patient 638 CBCS_N638


title: Ruvseq_Patient 639 CBCS_N639


title: Ruvseq_Patient 641 CBCS_N641


title: Ruvseq_Patient 636 CBCS_N636


title: Ruvseq_Patient 637 CBCS_N637


title: Ruvseq_Patient 635 CBCS_N635


title: 33028: M11_Nkx_Hi_wt


title: 33027: M11_Nkx_Hi_patUPD


title: 33026: M11_Nkx_Hi_matUPD


title: Ruvseq_Patient 633 CBCS_N633


title: 33038: M7_Emx_Hi_patUPD


title: Ruvseq_Patient 631 CBCS_N631


title: Ruvseq_Patient 642 CBCS_N642


title: 33024: M11_Nkx_Hi_wt


title: Ruvseq_Patient 630 CBCS_N630


title: 33023: M11_Nkx_Hi_patUPD


title: 33031: M7_Emx_Hi_patUPD


title: Ruvseq_Patient 673 CBCS_N673


title: 37770: M12_Emx_Hi_patUPD


title: 33030: M7_Emx_Hi_matUPD


title: Ruvseq_Patient 628 CBCS_N628


title: Ruvseq_Patient 629 CBCS_N629


title: 33019: M11_Nkx_Hi_matUPD


title: Ruvseq_Patient 634 CBCS_N634


title: Ruvseq_Patient 627 CBCS_N627


title: 33040: M7_Emx_Hi_wt


title: Ruvseq_Patient 632 CBCS_N632


title: 33016: M11_Nkx_Hi_wt


title: Ruvseq_Patient 626 CBCS_N626


title: Ruvseq_Patient 625 CBCS_N625


title: Ruvseq_Patient 692 CBCS_N692


title: 33014: M11_Nkx_Hi_patUPD


title: 33015: M11_Nkx_Hi_matUPD


title: Ruvseq_Patient 623 CBCS_N623


title: 32274: M7_Emx_Cx_wt

value: Neocortex


title: Ruvseq_Patient 622 CBCS_N622


title: 33022: M11_Nkx_Hi_matUPD

value: Nkx2.1 lineage


title: Ruvseq_Patient 621 CBCS_N621


title: 33020: M11_Nkx_Hi_wt

value: Hippocampus


title: 32273: M7_Emx_Cx_matUPD


title: 33018: M11_Nkx_Hi_patUPD

value: pat


title: Ruvseq_Patient 620 CBCS_N620


title: 32272: M7_Emx_Cx_patUPD


title: 32270: M7_Emx_Cx_wt


title: 32269: M7_Emx_Cx_matUPD

value: green


title: Ruvseq_Patient 618 CBCS_N618


title: 32268: M7_Emx_Cx_patUPD


title: Ruvseq_Patient 624 CBCS_N624


title: 32266: M7_Emx_Cx_wt


title: 32264: M7_Emx_Cx_matUPD

value: mat


title: 32265: M7_Emx_Cx_patUPD


title: Ruvseq_Patient 616 CBCS_N616


title: Ruvseq_Patient 617 CBCS_N617


title: Ruvseq_Patient 615 CBCS_N615


title: 32261: M7_Emx_Cx_patUPD


title: Ruvseq_Patient 614 CBCS_N614


title: Ruvseq_Patient 613 CBCS_N613


title: Ruvseq_Patient 612 CBCS_N612


title: 32260: M7_Emx_Cx_matUPD


title: Ruvseq_Patient 611 CBCS_N611


title: 32258: M11_Nkx_Cx_wt

value: yellow


title: 33034: M7_Emx_Hi_matUPD


title: Ruvseq_Patient 610 CBCS_N610


title: 32257: M11_Nkx_Cx_patUPD


title: 32256: M11_Nkx_Cx_matUPD


title: Ruvseq_Patient 609 CBCS_N609


title: Ruvseq_Patient 608 CBCS_N608


title: Ruvseq_Patient 619 CBCS_N619


title: 32254: M11_Nkx_Cx_wt

value: wt


title: Ruvseq_Patient 607 CBCS_N607


title: 32262: M7_Emx_Cx_wt


title: 32252: M11_Nkx_Cx_patUPD

value: red


title: Ruvseq_Patient 606 CBCS_N606

value: primary breast tumor


title: 32253: M11_Nkx_Cx_matUPD


title: Ruvseq_Patient 605 CBCS_N605

value: Negative


title: 32250: M11_Nkx_Cx_wt


title: Ruvseq_Patient 604 CBCS_N604


title: 32249: M11_Nkx_Cx_patUPD


title: 32248: M11_Nkx_Cx_matUPD


title: Ruvseq_Patient 603 CBCS_N603


title: Ruvseq_Patient 601 CBCS_N601


title: 32246: M11_Nkx_Cx_wt


title: 32245: M11_Nkx_Cx_matUPD


title: 32244: M11_Nkx_Cx_patUPD


title: Ruvseq_Patient 602 CBCS_N602


title: 27774: M11_Emx_Cx_wt


title: 27773: M11_Emx_Cx_matUPD


title: Ruvseq_Patient 599 CBCS_N599


title: 27770: M11_Emx_Cx_wt


title: 27772: M11_Emx_Cx_patUPD


title: Ruvseq_Patient 597 CBCS_N597


title: 27769: M11_Emx_Cx_matUPD


title: Ruvseq_Patient 596 CBCS_N596


title: 34989: M7_Nkx_Cx_patUPD


title: 27768: M11_Emx_Cx_patUPD


title: Ruvseq_Patient 595 CBCS_N595


title: Ruvseq_Patient 594 CBCS_N594


title: Ruvseq_Patient 658 CBCS_N658


title: 27766: M11_Emx_Cx_wt


title: Ruvseq_Patient 593 CBCS_N593


title: 27765: M11_Emx_Cx_patUPD


title: Ruvseq_Patient 592 CBCS_N592


title: Ruvseq_Patient 600 CBCS_N600


title: 27764: M11_Emx_Cx_matUPD


title: 27762: M11_Emx_Cx_wt


title: Ruvseq_Patient 591 CBCS_N591


title: 27761: M11_Emx_Cx_patUPD


title: 27760: M11_Emx_Cx_matUPD


title: Ruvseq_Patient 589 CBCS_N589


title: Ruvseq_Patient 598 CBCS_N598


title: Ruvseq_Patient 587 CBCS_N587


title: 113277: M7_astrocyte_P14_pat

data_processing:
Raw reads were delivered as BAM files and converted to fastq format using bamToFastq (bedtools suite v2.26.0) for alignment. Reads within transcripts were counted using STAR_2.5.0c with an index prepared from GRCm38 and Gencode M16 gene annotation. Note that for optimal representation of the long non-coding RNA Meg3 the spliced ENSMUST00000143836.7 transcript was modified to represent a single exon transcript. Parameters used for STAR alignment: --outFilterMultimapNmax 1 --outSAMstrandField intronMotif --outFilterIntronMotifs RemoveNoncanonical --outSAMtype BAM SortedByCoordinate --quantMode GeneCounts.
Raw counts from STAR alignment were used to analyse the data with DESeq2 (v1.26.0)
Genome_build: mm10/GRCm38
Supplementary_files_format_and_content: Read counts as reported by STAR as tab delimited text

growth_protocol: All mouse colonies were maintained in accordance with protocols approved by institutional animal care and use committee, institutional ethics committee and the preclinical core facility (PCF) at IST Austria. Experiments were performed under a license approved by the Austrian Federal Ministry of Science and Research in accordance with the Austrian and EU animal laws. Mouse lines used: MADM-7-GT (JAX stock # 021457), MADM-7-TG (JAX stock # 021458), Emx1-Cre (JAX stock # 005628), XGFAP-LacZ (JAX stock #003487)

extract_protocol:
Up to 400 cells were collected at 4°C and transferred into 96 well plates (Bio-Rad). Cells were sorted in 4µl lysis buffer (0.2% Triton X-100, 2U/µl RNase Inhibitor [Clonetech]). 96 well plates were kept on dry ice for sample transfer and stored at -80°C until further processing.
SMART-Seq2 libraries were prepared from cells (VBC GmbH, Vienna)

treatment_protocol: Animals were sacrificed and neocortices were dissected. Note that cortices of single P0 and P14 animals were processed as individual replicates. Single cell suspensions were prepared by using Papain containing L-cysteine and EDTA (vial 2, Worthington), DNase I (vial 3, Worthington), Ovomucoid protease inhibitor (vial 4, Worthington), EBSS (Thermo Fisher Scientific), DMEM/F12 (Thermo Fisher Scientific), FBS and HS. All vials from Worthington kit were reconstituted according to the manufacturer’s instructions using EBSS. The dissected brain areas were directly placed into Papain-DNase solution (20units/ml papain and 1000 units DNase). Enzymatic digestion was performed for 30min at 37°C in a shaking water bath. Next, solution 2 (EBSS containing 0.67mg Ovomucoid protease inhibitor and 166.7 U/ml DNase I) was added, the whole suspension was thoroughly mixed and centrifuged for 5min at 1000rpm at RT. Supernatant was removed and cell pellet was resuspended in solution 2. Trituration with p1000 pipette was performed to mechanically dissolve any remaining tissue parts. DMEM/F12 was added to the cell suspension as a washing solution, followed by a centrifugation step of 8min with 1500rpm at RT. The LacZ staining was performed using FACS Blue LacZ betaGalactosidase Detection Kit (Abcam). LacZ substrate stock solution was prepared according to the instruction manual. To generate the staining solution LacZ substrate was diluted 1:50 with DMEM/F12 (Thermo Fisher Scientific). Cell pellet was resuspended with 100µl of LacZ staining solution and incubated for 25min at 37°C on a thermoshaker. Staining reaction was stopped by adding 100µl of media (DMEM/F12 containing 10% FBS and 10% HS) followed by a centrifugation step (1500rpm for 8min). Supernatant was removed and cells were resuspended in media. Samples were kept in the dark at room temperature until sorting was started.

value: astrocyte

value: R


title: Ruvseq_Patient 586 CBCS_N586


title: 113274: M7_astrocyte_P14_mat

value: G


title: 113273: M7_astrocyte_P14_pat


title: Ruvseq_Patient 585 CBCS_N585

value: Basal


title: 113269: M7_astrocyte_P14_pat


title: Ruvseq_Patient 584 CBCS_N584


title: Ruvseq_Patient 582 CBCS_N582


title: Ruvseq_Patient 583 CBCS_N583


title: 113266: M7_astrocyte_P14_mat


title: 113265: M7_astrocyte_P14_pat


title: 113262: M7_astrocyte_P14_mat


title: Ruvseq_Patient 590 CBCS_N590


title: Ruvseq_Patient 581 CBCS_N581


title: Ruvseq_Patient 580 CBCS_N580


title: 113258: M7_astrocyte_P14_mat


title: 113257: M7_astrocyte_P14_pat


title: Ruvseq_Patient 579 CBCS_N579


title: Ruvseq_Patient 588 CBCS_N588


title: Ruvseq_Patient 578 CBCS_N578

value: LumB


title: 113261: M7_astrocyte_P14_pat


title: 113237: M7_neuron_P0_pat

value: neuron


title: Ruvseq_Patient 574 CBCS_N574


title: 113270: M7_astrocyte_P14_mat

value: Neocortex


title: Ruvseq_Patient 576 CBCS_N576


title: Ruvseq_Patient 577 CBCS_N577


title: 113235: M7_astrocyte_P0_mat


title: 113236: M7_astrocyte_P0_pat


title: Ruvseq_Patient 573 CBCS_N573

value: LumA


title: 113234: M7_neuron_P0_mat


title: Ruvseq_Patient 572 CBCS_N572


title: Ruvseq_Patient 575 CBCS_N575


title: Ruvseq_Patient 571 CBCS_N571


title: Ruvseq_Patient 570 CBCS_N570


title: 113233: M7_neuron_P0_pat


title: Ruvseq_Patient 569 CBCS_N569


title: 113231: M7_astrocyte_P0_mat


title: 113232: M7_astrocyte_P0_pat


title: 113230: M7_neuron_P0_mat


title: 113254: M7_astrocyte_P14_mat


title: Ruvseq_Patient 568 CBCS_N568


title: Ruvseq_Patient 567 CBCS_N567


title: Ruvseq_Patient 566 CBCS_N566


title: 113228: M7_astrocyte_P0_pat


title: 113227: M7_astrocyte_P0_mat


title: 113226: M7_neuron_P0_mat


title: Ruvseq_Patient 565 CBCS_N565


title: 113225: M7_neuron_P0_pat


title: Ruvseq_Patient 563 CBCS_N563


title: Ruvseq_Patient 562 CBCS_N562


title: 113223: M7_astrocyte_P0_mat


title: 113224: M7_astrocyte_P0_pat


title: Ruvseq_Patient 560 CBCS_N560


title: Ruvseq_Patient 559 CBCS_N559


title: Ruvseq_Patient 558 CBCS_N558


title: Ruvseq_Patient 557 CBCS_N557


title: 113229: M7_neuron_P0_pat


title: Ruvseq_Patient 556 CBCS_N556


title: Cast_C57/Bl6_Cx_Emx_2

data_processing:
Twenty million reads (counting from the start of each BAM file) were aligned with STAR_2.5.0c using the following parameters: --clip5pNbases 3, --outFilterMultimapNmax 1, --runThreadN 2, --outSAMtype BAM, SortedByCoordinate, --limitBAMsortRAM 3000000000, --quantMode GeneCounts. NOTE: for paired end sequencings only reverse reads were used.
Allelic expression: BAM files were separated by strand using the “separate_BAM_strand.pl” from the Allelome.PRO package (PMID: ) using the ‘++,--‘ strand rule. The resulting BAM files were analyzed using Allelome.PRO with standard parameters (allelic ratio cutoff: 0.7, FDR: 1%). SNP data was obtained from ftp://ftp-mouse.sanger.ac.uk (mgp.v3.snps.rsIDdbSNPv137.vcf.bz2). As an annotation Gencode M16 was used.
Genome_build: mm10/GRCm38
Supplementary_files_format_and_content: Read counts in transcripts as reported by STAR as tab delimited text
Supplementary_files_format_and_content: zip container with allelic expression quantification for each tissue/cell type as reported by Allelome.PRO

growth_protocol: All mouse colonies were maintained in accordance with protocols approved by institutional animal care and use committee, institutional ethics committee and the preclinical core facility (PCF) at IST Austria. Experiments were performed under a license approved by the Austrian Federal Ministry of Science and Research in accordance with the Austrian and EU animal laws. Mouse lines used: Emx1-Cre REF (JAX stock # 005628) REF; Nkx2.1-Cre (JAX stock # 008661); Z/EG (JAX stock # 004178); Ai14 (JAX stock # 007914); FVB (JAX stock # 001800); CAST/EiJ (JAX stock # 000928) and C57Bl/6J (JAX stock # 000664)

extract_protocol:
Whole tissue samples were processed according to the Trizol LS protocol. All other samples were incubated for 30min at 37°C. Total volume was filled to 250µl using RNase-free H2O (Thermo Fisher Scientific) followed by addition of 750µl Trizol LS (Thermo Fisher Scientific). Samples were mixed by 5 times inverting. After a 5min incubation step at RT, the entire solution was transferred into a MaXtract tube (Qiagen). 200µl chloroform (Sigma-Aldrich) was added, followed by 3 times 5sec vortexing and 2min incubation at RT. Samples were centrifuged for 2min with 12000rpm at 18°C. Supernatant was transferred to a new tube and isopropanol (Sigma-Aldrich) was added in a 1:1 ratio. For better visibility of the RNA pellet 1µl GlycoBlue (Thermo Fisher Scientific) was added and entire solution was mixed by vortexing (3x 5sec). Samples were left for precipitation o/n at -20°C. After precipitation samples were centrifuged for 20min with 14000rpm at 4°C. Supernatant was removed and RNA pellet was washed with 70% ethanol, followed by a 5min centrifugation step (14000rpm at 4°C). RNA pellet was resuspended in an appropriate amount of RNase-free H2O. RNA quality was analyzed using Bioanalyzer RNA 6000 Nano (Agilent) and RNA 6000 Pico kit (Agilent) following the manufacturer’s instructions.
500ng of total RNA were treated with DNase1 (Applied Biosystems) and purified using Ampure RNAClean XP beads. 200ng of the purified RNA was used for library preparation using the Illumina TruSeq Stranded Total RNA LT - (with Ribo-Zero TM Gold) - according to the manufacturer’s protocol. 7-40ng of total RNA from FACS sorted cells were used for library preparation with the Clonetech SMARTer Stranded Total RNA Sample Prep Kit – Low Input Mammalian (with Ribogone), according to the manufacturer’s protocol with 19 cycles of library amplification. If the concentration of the total RNA sample was too low, RNA was immobilized on Agencourt RNAclean XP beads according to the manufacturer’s protocol. After washing and drying of the beads the RNA was eluted in 5yl water and the whole volume (including beads) was used in the RiboGone reaction.

treatment_protocol: P0 animals were sacrificed and brain areas of interest (neocortex, hippocampus and olfactory bulb) were dissected. Whole tissue samples were directly placed in TriZol LS. Single cell suspensions were prepared by using Papain containing L-cysteine and EDTA (vial 2, Worthington), DNase I (vial 3, Worthington), Ovomucoid protease inhibitor (vial 4, Worthington), EBSS (Thermo Fisher Scientific), DMEM/F12 (Thermo Fisher Scientific), FBS and HS. All vials from Worthington kit were reconstituted according to the manufacturer’s instructions using EBSS. The dissected brain areas were directly placed into Papain-DNase solution (20units/ml papain and 1000 units DNase). Entire litters were pooled in order to obtain sufficient material for RNA extraction. Enzymatic digestion was performed for 30min at 37°C in a shaking water bath. Next, solution 2 (EBSS containing 0.67mg Ovomucoid protease inhibitor and 166.7 U/ml DNase I) was added, the whole suspension was thoroughly mixed and centrifuged for 5min at 1000rpm at RT. Supernatant was removed and cell pellet was resuspended in solution 2. Trituration with p1000 pipette was performed to mechanically dissolve any remaining tissue parts. DMEM/F12 was added to the cell suspension as a washing solution, followed by a centrifugation step of 5min with 1500rpm at RT. Cells were resuspended in media (DMEM/F12 containing 10% FBS and 10% HS) and kept on ice until sorted. Right before sorting, cell suspension was filtered using a 40µm cell strainer. FACS was performed on a BD FACS Aria III using 100 nozzle and keeping sample and collection devices at 4°C. Duplet exclusion was performed to ensure sorting of true single cells. Cells were sorted directly into either Isolation Buffer (IB; Lexogen) if more than 2000 sorted cells/per color were expected or if samples yielded fewer labeled cells into custom made lysis buffer (30nM TRIS pH 8, 10nM EDTA pH 8, 1% SDS and 200 µg/µL Proteinase K).

value: neurons from Emx1 lineage

value: neocortex

tag: mother strain

value: Cast

tag: father strain

tag: cre-driver

tag: inducible marker

value: eGFP

tag: sequencing kit


title: Cast_C57/Bl6_Hi_Emx_2

value: hippocampus

value: SMARTer Stranded Total RNA Sample Prep Kit


title: Cast_C57/Bl6_OB_Emx_1

value: olfactory bulb


title: Cast_C57/Bl6_Hi_Emx_1


title: Ruvseq_Patient 555 CBCS_N555

value: primary breast tumor


title: Ruvseq_Patient 554 CBCS_N554

value: Negative


title: Cast_C57/Bl6_Cx_Emx_1


title: Ruvseq_Patient 553 CBCS_N553


title: Ruvseq_Patient 564 CBCS_N564


title: Cast_C57/Bl6_OB_Emx_2


title: Ruvseq_Patient 561 CBCS_N561


title: C57/Bl6_Cast_Hi_Emx_2


title: 113222: M7_neuron_P0_mat

value: mat


title: C57/Bl6_Cast_Cx_Emx_2


title: Ruvseq_Patient 551 CBCS_N551


title: C57/Bl6_Cast_Cx_Emx_1


title: C57/Bl6_Cast_OB_Emx_1


title: C57/Bl6_Cast_Hi_Emx_1


title: Ruvseq_Patient 550 CBCS_N550


title: C57/Bl6_Cast_Hi_Nkx_2

value: neurons from Nkx2.1 lineage

value: tdTomato


title: Ruvseq_Patient 549 CBCS_N549


title: Ruvseq_Patient 548 CBCS_N548

value: Basal


title: Ruvseq_Patient 552 CBCS_N552


title: Ruvseq_Patient 547 CBCS_N547


title: Ruvseq_Patient 546 CBCS_N546


title: C57/Bl6_Cast_Hi_Nkx_1


title: Ruvseq_Patient 544 CBCS_N544


title: Ruvseq_Patient 545 CBCS_N545


title: Cast_C57/Bl6_Hi_Nkx_1


title: C57/Bl6_Cast_OB_Emx_2


title: Ruvseq_Patient 541 CBCS_N541


title: Ruvseq_Patient 542 CBCS_N542


title: Ruvseq_Patient 543 CBCS_N543


title: C57/Bl6_Cast_Cx_Nkx_1


title: C57/Bl6_Cast_Cx_Nkx_2


title: Ruvseq_Patient 539 CBCS_N539


title: Cast_C57/Bl6_Cx_Nkx_2


title: Ruvseq_Patient 540 CBCS_N540


title: Cast_C57/Bl6_Cx_Nkx_1


title: Cast_FVB_OB_whole_tissue_1

value: whole tissue

value: FVB

value: TruSeq


title: Cast_FVB_OB_whole_tissue_2


title: Cast_FVB_Hi_whole_tissue_1


title: Cast_FVB_Hi_whole_tissue_2


title: Ruvseq_Patient 537 CBCS_N537

value: LumA


title: Cast_FVB_Cx_whole_tissue_2


title: Ruvseq_Patient 538 CBCS_N538


title: Cast_C57/Bl6_Hi_Nkx_2


title: Cast_FVB_Cx_whole_tissue_1


title: FVB_Cast_OB_whole_tissue_1


title: FVB_Cast_OB_whole_tissue_2


title: Ruvseq_Patient 536 CBCS_N536


title: FVB_Cast_Hi_whole_tissue_2


title: Ruvseq_Patient 535 CBCS_N535


title: Ruvseq_Patient 532 CBCS_N532


title: Ruvseq_Patient 534 CBCS_N534


title: Ruvseq_Patient 531 CBCS_N531


title: FVB_Cast_Cx_whole_tissue_2


title: FVB_Cast_Hi_whole_tissue_1


title: Ruvseq_Patient 529 CBCS_N529


title: S17447_M7_Emx_Cx_P0_wt

data_processing:
Raw reads were delivered as BAM files and converted to fastq format using bamToFastq (bedtools suite v2.26.0) for alignment. Reads within transcripts were counted using STAR_2.5.0c with an index prepared from GRCm38 and Gencode M16 gene annotation. Note that for optimal representation of the long non-coding RNA Meg3 the spliced ENSMUST00000143836.7 transcript was modified to represent a single exon transcript. Parameters used for STAR alignment: --outFilterMultimapNmax 1 --outSAMstrandField intronMotif --outFilterIntronMotifs RemoveNoncanonical --outSAMtype BAM SortedByCoordinate --quantMode GeneCounts.
Raw counts from STAR alignment were used to analyse the data with Seurat (v3.1.4)
Genome_build: mm10/GRCm38
Supplementary_files_format_and_content: Read counts in transcripts as reported by STAR as tab delimited text

growth_protocol: All mouse colonies were maintained in accordance with protocols approved by institutional animal care and use committee, institutional ethics committee and the preclinical core facility (PCF) at IST Austria. Experiments were performed under a license approved by the Austrian Federal Ministry of Science and Research in accordance with the Austrian and EU animal laws. Mouse lines used: Emx1-Cre (JAX stock # 005628); MADM-7-GT (JAX stock # 021457), MADM-7-TG (JAX stock # 021458)

extract_protocol:
Single cells were sorted into 96 well plates (Bio-Rad) containing 4µl lysis buffer (0.2% Triton X-100, 2U/µl RNase Inhibitor [Clonetech]) per well.
sorted cells were directly processed without RNA extraction using Smart-Seq2 (VBCF GmbH Vienna or Biomedical Sequencing Facility (BSF) at CeMM Vienna)

treatment_protocol: Animals were sacrificed and brain areas of interest were dissected. Single cell suspensions were prepared by using Papain containing L-cysteine and EDTA (vial 2, Worthington), DNase I (vial 3, Worthington), Ovomucoid protease inhibitor (vial 4, Worthington), EBSS (Thermo Fisher Scientific), DMEM/F12 (Thermo Fisher Scientific), FBS and HS. All vials from Worthington kit were reconstituted according to the manufacturer’s instructions using EBSS. The dissected brain areas were directly placed into Papain-DNase solution (20units/ml papain and 1000 units DNase). Enzymatic digestion was performed for 30min at 37°C in a shaking water bath. Next, solution 2 (EBSS containing 0.67mg Ovomucoid protease inhibitor and 166.7 U/ml DNase I) was added, the whole suspension was thoroughly mixed and centrifuged for 5min at 1000rpm at RT. Supernatant was removed and cell pellet was resuspended in solution 2. Trituration with p1000 pipette was performed to mechanically dissolve any remaining tissue parts. DMEM/F12 was added to the cell suspension as a washing solution, followed by a centrifugation step of 5min with 1500rpm at RT. Cells were resuspended in media (DMEM/F12 containing 10% FBS and 10% HS) and kept on ice until sorted. Right before sorting, cell suspension was filtered using a 40µm cell strainer. FACS was performed on a BD FACS Aria III using 100 nozzle and keeping sample and collection devices at 4°C. Duplet exclusion was performed to ensure sorting of true single cells. GFP+ (green), tdTomato+ (red), and GFP+/tdTomato+ (yellow) cells were collected.

value: Emx1 lineage

value: Neocortex

value: wt


title: Ruvseq_Patient 528 CBCS_N528


title: FVB_Cast_Cx_whole_tissue_1

value: neocortex

value: Cast


title: Ruvseq_Patient 527 CBCS_N527


title: Ruvseq_Patient 526 CBCS_N526


title: Ruvseq_Patient 525 CBCS_N525


title: S17444_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 533 CBCS_N533

value: LumB


title: S17443_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 524 CBCS_N524


title: S17445_M7_Emx_Cx_P0_wt


title: S17448_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 523 CBCS_N523


title: S17441_M7_Emx_Cx_P0_wt


title: S17442_M7_Emx_Cx_P0_wt


title: S17440_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 521 CBCS_N521


title: Ruvseq_Patient 522 CBCS_N522


title: Ruvseq_Patient 520 CBCS_N520


title: Ruvseq_Patient 519 CBCS_N519


title: Ruvseq_Patient 518 CBCS_N518


title: S17439_M7_Emx_Cx_P0_wt


title: S17438_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 516 CBCS_N516


title: S17446_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 515 CBCS_N515


title: Ruvseq_Patient 530 CBCS_N530


title: Ruvseq_Patient 513 CBCS_N513

value: Normal


title: S17435_M7_Emx_Cx_P0_wt


title: S17436_M7_Emx_Cx_P0_wt


title: S17437_M7_Emx_Cx_P0_wt


title: S17434_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 512 CBCS_N512

value: primary breast tumor


title: S17433_M7_Emx_Cx_P0_wt


title: S17432_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 511 CBCS_N511

value: Negative


title: S17431_M7_Emx_Cx_P0_wt


title: S17430_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 510 CBCS_N510


title: S17429_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 509 CBCS_N509


title: Ruvseq_Patient 517 CBCS_N517


title: S17426_M7_Emx_Cx_P0_wt


title: S17428_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 508 CBCS_N508


title: Ruvseq_Patient 505 CBCS_N505


title: Ruvseq_Patient 503 CBCS_N503


title: Ruvseq_Patient 504 CBCS_N504


title: Ruvseq_Patient 514 CBCS_N514


title: S17427_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 506 CBCS_N506


title: S17424_M7_Emx_Cx_P0_wt


title: S17423_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 502 CBCS_N502


title: S17422_M7_Emx_Cx_P0_wt


title: S17420_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 501 CBCS_N501


title: S17421_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 499 CBCS_N499


title: S17419_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 507 CBCS_N507


title: Ruvseq_Patient 500 CBCS_N500


title: Ruvseq_Patient 498 CBCS_N498


title: S17417_M7_Emx_Cx_P0_wt


title: S17418_M7_Emx_Cx_P0_wt


title: S17416_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 497 CBCS_N497


title: Ruvseq_Patient 495 CBCS_N495


title: Ruvseq_Patient 494 CBCS_N494


title: S17415_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 496 CBCS_N496


title: S17414_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 493 CBCS_N493


title: S17412_M7_Emx_Cx_P0_wt


title: S17413_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 491 CBCS_N491


title: S17410_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 489 CBCS_N489


title: Ruvseq_Patient 490 CBCS_N490


title: Ruvseq_Patient 488 CBCS_N488


title: S17409_M7_Emx_Cx_P0_wt


title: S17407_M7_Emx_Cx_P0_wt


title: S17406_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 487 CBCS_N487


title: Ruvseq_Patient 486 CBCS_N486


title: Ruvseq_Patient 485 CBCS_N485


title: S17403_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 483 CBCS_N483


title: S17402_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 484 CBCS_N484


title: Ruvseq_Patient 481 CBCS_N481


title: Ruvseq_Patient 492 CBCS_N492


title: Ruvseq_Patient 482 CBCS_N482


title: S17411_M7_Emx_Cx_P0_wt


title: S17400_M7_Emx_Cx_P0_mat

value: mat


title: Ruvseq_Patient 478 CBCS_N478


title: S17399_M7_Emx_Cx_P0_mat


title: Ruvseq_Patient 479 CBCS_N479


title: Ruvseq_Patient 480 CBCS_N480


title: Ruvseq_Patient 477 CBCS_N477


title: S17408_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 476 CBCS_N476


title: S17398_M7_Emx_Cx_P0_mat


title: Ruvseq_Patient 475 CBCS_N475


title: S17396_M7_Emx_Cx_P0_mat


title: S17405_M7_Emx_Cx_P0_wt


title: S17404_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 474 CBCS_N474


title: S17395_M7_Emx_Cx_P0_mat


title: S17401_M7_Emx_Cx_P0_wt


title: S17393_M7_Emx_Cx_P0_mat


title: Ruvseq_Patient 472 CBCS_N472


title: Ruvseq_Patient 473 CBCS_N473


title: S17392_M7_Emx_Cx_P0_mat


title: S17390_M7_Emx_Cx_P0_mat


title: Ruvseq_Patient 471 CBCS_N471


title: S17391_M7_Emx_Cx_P0_mat


title: S17389_M7_Emx_Cx_P0_mat


title: S17387_M7_Emx_Cx_P0_mat


title: S17397_M7_Emx_Cx_P0_mat


title: S17386_M7_Emx_Cx_P0_mat


title: Ruvseq_Patient 469 CBCS_N469


title: Ruvseq_Patient 464 CBCS_N464


title: Ruvseq_Patient 467 CBCS_N467


title: Ruvseq_Patient 468 CBCS_N468


title: Ruvseq_Patient 466 CBCS_N466


title: Ruvseq_Patient 465 CBCS_N465


title: S17383_M7_Emx_Cx_P0_mat


title: Ruvseq_Patient 462 CBCS_N462


title: S17382_M7_Emx_Cx_P0_mat


title: Ruvseq_Patient 463 CBCS_N463


title: S17385_M7_Emx_Cx_P0_mat


title: Ruvseq_Patient 461 CBCS_N461


title: S17394_M7_Emx_Cx_P0_mat


title: Ruvseq_Patient 470 CBCS_N470


title: Ruvseq_Patient 460 CBCS_N460


title: S17381_M7_Emx_Cx_P0_mat


title: S17388_M7_Emx_Cx_P0_mat


title: S17379_M7_Emx_Cx_P0_mat


title: S17380_M7_Emx_Cx_P0_mat


title: S17378_M7_Emx_Cx_P0_mat


title: Ruvseq_Patient 458 CBCS_N458


title: Ruvseq_Patient 459 CBCS_N459


title: Ruvseq_Patient 456 CBCS_N456


title: S17384_M7_Emx_Cx_P0_mat


title: Ruvseq_Patient 457 CBCS_N457


title: Ruvseq_Patient 455 CBCS_N455


title: Ruvseq_Patient 454 CBCS_N454


title: S17377_M7_Emx_Cx_P0_mat


title: S17376_M7_Emx_Cx_P0_mat


title: S17375_M7_Emx_Cx_P0_mat


title: S17374_M7_Emx_Cx_P0_mat


title: S17373_M7_Emx_Cx_P0_mat


title: Ruvseq_Patient 453 CBCS_N453


title: Ruvseq_Patient 452 CBCS_N452


title: S17372_M7_Emx_Cx_P0_mat


title: S17371_M7_Emx_Cx_P0_mat


title: S17370_M7_Emx_Cx_P0_mat


title: Ruvseq_Patient 450 CBCS_N450


title: Ruvseq_Patient 451 CBCS_N451


title: S17369_M7_Emx_Cx_P0_mat


title: S17368_M7_Emx_Cx_P0_mat


title: Ruvseq_Patient 449 CBCS_N449


title: S17367_M7_Emx_Cx_P0_mat


title: Ruvseq_Patient 448 CBCS_N448


title: S17365_M7_Emx_Cx_P0_mat


title: S17366_M7_Emx_Cx_P0_mat


title: Ruvseq_Patient 446 CBCS_N446


title: S17364_M7_Emx_Cx_P0_mat


title: Ruvseq_Patient 447 CBCS_N447


title: Ruvseq_Patient 444 CBCS_N444


title: Ruvseq_Patient 445 CBCS_N445


title: Ruvseq_Patient 443 CBCS_N443


title: S17363_M7_Emx_Cx_P0_mat


title: S17361_M7_Emx_Cx_P0_mat


title: S17360_M7_Emx_Cx_P0_mat


title: Ruvseq_Patient 441 CBCS_N441


title: Ruvseq_Patient 439 CBCS_N439


title: Ruvseq_Patient 440 CBCS_N440


title: S17359_M7_Emx_Cx_P0_mat


title: S17358_M7_Emx_Cx_P0_mat


title: S17357_M7_Emx_Cx_P0_mat


title: S17355_M7_Emx_Cx_P0_mat


title: S17356_M7_Emx_Cx_P0_mat


title: Ruvseq_Patient 442 CBCS_N442


title: S17352_M7_Emx_Cx_P0_pat

value: pat


title: Ruvseq_Patient 435 CBCS_N435


title: Ruvseq_Patient 438 CBCS_N438


title: S17354_M7_Emx_Cx_P0_mat


title: Ruvseq_Patient 434 CBCS_N434


title: Ruvseq_Patient 436 CBCS_N436


title: Ruvseq_Patient 437 CBCS_N437


title: S17353_M7_Emx_Cx_P0_mat


title: S17351_M7_Emx_Cx_P0_pat


title: S17362_M7_Emx_Cx_P0_mat


title: Ruvseq_Patient 432 CBCS_N432


title: Ruvseq_Patient 433 CBCS_N433


title: Ruvseq_Patient 429 CBCS_N429


title: Ruvseq_Patient 430 CBCS_N430


title: Ruvseq_Patient 431 CBCS_N431


title: Ruvseq_Patient 428 CBCS_N428


title: S17349_M7_Emx_Cx_P0_pat


title: S17347_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 427 CBCS_N427


title: S17345_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 425 CBCS_N425


title: Ruvseq_Patient 423 CBCS_N423


title: S17344_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 424 CBCS_N424


title: Ruvseq_Patient 422 CBCS_N422


title: S17350_M7_Emx_Cx_P0_pat


title: S17342_M7_Emx_Cx_P0_pat


title: S17343_M7_Emx_Cx_P0_pat


title: S17341_M7_Emx_Cx_P0_pat


title: S17340_M7_Emx_Cx_P0_pat


title: S17339_M7_Emx_Cx_P0_pat


title: S17338_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 421 CBCS_N421


title: Ruvseq_Patient 420 CBCS_N420


title: Ruvseq_Patient 426 CBCS_N426


title: S17348_M7_Emx_Cx_P0_pat


title: S17346_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 416 CBCS_N416


title: Ruvseq_Patient 419 CBCS_N419


title: S17337_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 418 CBCS_N418


title: S17336_M7_Emx_Cx_P0_pat


title: S17335_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 415 CBCS_N415


title: Ruvseq_Patient 413 CBCS_N413


title: Ruvseq_Patient 414 CBCS_N414


title: Ruvseq_Patient 412 CBCS_N412


title: S17334_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 411 CBCS_N411


title: S17333_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 417 CBCS_N417


title: S17331_M7_Emx_Cx_P0_pat


title: S17332_M7_Emx_Cx_P0_pat


title: S17330_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 410 CBCS_N410


title: S17327_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 408 CBCS_N408


title: S17326_M7_Emx_Cx_P0_pat


title: S17325_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 409 CBCS_N409


title: Ruvseq_Patient 406 CBCS_N406


title: Ruvseq_Patient 407 CBCS_N407


title: S17324_M7_Emx_Cx_P0_pat


title: S17322_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 405 CBCS_N405


title: Ruvseq_Patient 402 CBCS_N402


title: S17323_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 401 CBCS_N401


title: Ruvseq_Patient 403 CBCS_N403


title: Ruvseq_Patient 404 CBCS_N404


title: Ruvseq_Patient 399 CBCS_N399


title: Ruvseq_Patient 400 CBCS_N400


title: S17321_M7_Emx_Cx_P0_pat


title: S17320_M7_Emx_Cx_P0_pat


title: S17329_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 398 CBCS_N398


title: S17328_M7_Emx_Cx_P0_pat


title: S17319_M7_Emx_Cx_P0_pat


title: S17318_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 397 CBCS_N397


title: S17317_M7_Emx_Cx_P0_pat


title: S17316_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 396 CBCS_N396


title: Ruvseq_Patient 395 CBCS_N395


title: S17314_M7_Emx_Cx_P0_pat


title: S17315_M7_Emx_Cx_P0_pat


title: S17313_M7_Emx_Cx_P0_pat


title: S17312_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 394 CBCS_N394


title: Ruvseq_Patient 392 CBCS_N392


title: Ruvseq_Patient 391 CBCS_N391


title: Ruvseq_Patient 390 CBCS_N390


title: S17309_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 389 CBCS_N389


title: Ruvseq_Patient 387 CBCS_N387


title: S17308_M7_Emx_Cx_P0_pat


title: S17307_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 388 CBCS_N388


title: Ruvseq_Patient 386 CBCS_N386


title: Ruvseq_Patient 385 CBCS_N385


title: S17306_M7_Emx_Cx_P0_pat


title: S17305_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 384 CBCS_N384


title: Ruvseq_Patient 383 CBCS_N383


title: S17304_M7_Emx_Cx_P0_wt


title: S17303_M7_Emx_Cx_P0_wt


title: S17302_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 393 CBCS_N393


title: S17310_M7_Emx_Cx_P0_pat


title: S17300_M7_Emx_Cx_P0_wt


title: S17311_M7_Emx_Cx_P0_pat


title: S17298_M7_Emx_Cx_P0_wt


title: S17301_M7_Emx_Cx_P0_wt


title: S17297_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 382 CBCS_N382


title: Ruvseq_Patient 381 CBCS_N381


title: Ruvseq_Patient 379 CBCS_N379


title: S17296_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 380 CBCS_N380


title: Ruvseq_Patient 377 CBCS_N377


title: Ruvseq_Patient 375 CBCS_N375


title: Ruvseq_Patient 374 CBCS_N374


title: S17295_M7_Emx_Cx_P0_wt


title: S17294_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 373 CBCS_N373


title: Ruvseq_Patient 376 CBCS_N376


title: S17293_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 371 CBCS_N371


title: Ruvseq_Patient 372 CBCS_N372


title: S17299_M7_Emx_Cx_P0_wt


title: S17292_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 370 CBCS_N370


title: S17291_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 378 CBCS_N378


title: S17290_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 369 CBCS_N369


title: S17289_M7_Emx_Cx_P0_wt


title: S17287_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 368 CBCS_N368


title: S17286_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 367 CBCS_N367


title: Ruvseq_Patient 365 CBCS_N365


title: S17285_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 366 CBCS_N366


title: Ruvseq_Patient 364 CBCS_N364


title: S17284_M7_Emx_Cx_P0_wt


title: S17283_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 362 CBCS_N362


title: Ruvseq_Patient 363 CBCS_N363


title: S17282_M7_Emx_Cx_P0_wt


title: S17281_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 361 CBCS_N361


title: Ruvseq_Patient 360 CBCS_N360


title: Ruvseq_Patient 358 CBCS_N358


title: S17279_M7_Emx_Cx_P0_wt


title: S17278_M7_Emx_Cx_P0_wt


title: S17277_M7_Emx_Cx_P0_wt


title: S17288_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 356 CBCS_N356


title: S17276_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 355 CBCS_N355


title: Ruvseq_Patient 354 CBCS_N354


title: Ruvseq_Patient 353 CBCS_N353


title: S17275_M7_Emx_Cx_P0_wt


title: S17274_M7_Emx_Cx_P0_wt


title: S17273_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 352 CBCS_N352


title: S17272_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 351 CBCS_N351


title: S17271_M7_Emx_Cx_P0_wt


title: S17270_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 350 CBCS_N350


title: Ruvseq_Patient 359 CBCS_N359


title: S17269_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 349 CBCS_N349


title: S17280_M7_Emx_Cx_P0_wt


title: S17268_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 348 CBCS_N348


title: S17267_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 347 CBCS_N347


title: Ruvseq_Patient 357 CBCS_N357


title: Ruvseq_Patient 346 CBCS_N346


title: Ruvseq_Patient 345 CBCS_N345

value: Weak Positive / Borderline


title: S17266_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 344 CBCS_N344

value: Unknown


title: S17265_M7_Emx_Cx_P0_wt


title: S17263_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 342 CBCS_N342


title: Ruvseq_Patient 341 CBCS_N341


title: S17264_M7_Emx_Cx_P0_wt


title: S17262_M7_Emx_Cx_P0_wt


title: S17261_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 343 CBCS_N343


title: Ruvseq_Patient 340 CBCS_N340

value: Positive


title: Ruvseq_Patient 339 CBCS_N339


title: S17260_M7_Emx_Cx_P0_wt


title: S17259_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 338 CBCS_N338


title: S17258_M7_Emx_Cx_P0_wt


title: Ruvseq_Patient 337 CBCS_N337


title: Ruvseq_Patient 336 CBCS_N336


title: S17256_M7_Emx_Cx_P0_pat


title: S17257_M7_Emx_Cx_P0_wt


title: S17255_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 334 CBCS_N334


title: Ruvseq_Patient 335 CBCS_N335


title: Ruvseq_Patient 333 CBCS_N333


title: S17253_M7_Emx_Cx_P0_pat


title: S17254_M7_Emx_Cx_P0_pat


title: S17252_M7_Emx_Cx_P0_pat


title: S17251_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 330 CBCS_N330


title: Ruvseq_Patient 331 CBCS_N331


title: Ruvseq_Patient 329 CBCS_N329


title: S17250_M7_Emx_Cx_P0_pat


title: S17248_M7_Emx_Cx_P0_pat


title: S17247_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 328 CBCS_N328


title: Ruvseq_Patient 326 CBCS_N326


title: S17246_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 325 CBCS_N325


title: S17244_M7_Emx_Cx_P0_pat


title: S17243_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 332 CBCS_N332


title: S17242_M7_Emx_Cx_P0_pat


title: S17241_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 321 CBCS_N321


title: Ruvseq_Patient 323 CBCS_N323

value: Basal


title: S17240_M7_Emx_Cx_P0_pat


title: S17239_M7_Emx_Cx_P0_pat


title: S17249_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 319 CBCS_N319


title: Ruvseq_Patient 318 CBCS_N318


title: Ruvseq_Patient 317 CBCS_N317


title: S17238_M7_Emx_Cx_P0_pat


title: S17237_M7_Emx_Cx_P0_pat


title: S17245_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 316 CBCS_N316


title: Ruvseq_Patient 327 CBCS_N327


title: Ruvseq_Patient 315 CBCS_N315


title: S17236_M7_Emx_Cx_P0_pat


title: S17235_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 324 CBCS_N324


title: Ruvseq_Patient 314 CBCS_N314


title: S17234_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 322 CBCS_N322


title: S17233_M7_Emx_Cx_P0_pat


title: S17232_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 320 CBCS_N320


title: S17231_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 311 CBCS_N311


title: Ruvseq_Patient 310 CBCS_N310


title: S17230_M7_Emx_Cx_P0_pat


title: S17229_M7_Emx_Cx_P0_pat


title: S17228_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 308 CBCS_N308


title: Ruvseq_Patient 307 CBCS_N307


title: Ruvseq_Patient 306 CBCS_N306


title: S17226_M7_Emx_Cx_P0_pat


title: S17225_M7_Emx_Cx_P0_pat


title: S17224_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 305 CBCS_N305


title: Ruvseq_Patient 313 CBCS_N313


title: Ruvseq_Patient 312 CBCS_N312


title: S17222_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 303 CBCS_N303


title: S17221_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 309 CBCS_N309


title: S17223_M7_Emx_Cx_P0_pat


title: S17220_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 299 CBCS_N299


title: Ruvseq_Patient 300 CBCS_N300


title: S17219_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 298 CBCS_N298


title: Ruvseq_Patient 301 CBCS_N301


title: S17218_M7_Emx_Cx_P0_pat


title: S17227_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 297 CBCS_N297


title: S17217_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 296 CBCS_N296


title: Ruvseq_Patient 304 CBCS_N304


title: S17216_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 295 CBCS_N295


title: S17215_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 302 CBCS_N302


title: S17214_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 294 CBCS_N294


title: Ruvseq_Patient 292 CBCS_N292


title: Ruvseq_Patient 293 CBCS_N293


title: S17213_M7_Emx_Cx_P0_pat


title: S17212_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 291 CBCS_N291


title: S17211_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 290 CBCS_N290


title: S17210_M7_Emx_Cx_P0_pat


title: Ruvseq_Patient 288 CBCS_N288


title: Ruvseq_Patient 289 CBCS_N289


title: S17209_M7_Emx_Cx_P0_pat


title: S17208_M7_Emx_Cx_P0_mat


title: S17207_M7_Emx_Cx_P0_mat


title: S17206_M7_Emx_Cx_P0_mat


title: Ruvseq_Patient 287 CBCS_N287


title: S17205_M7_Emx_Cx_P0_mat


title: S17203_M7_Emx_Cx_P0_mat


title: S17204_M7_Emx_Cx_P0_mat


title: Ruvseq_Patient 286 CBCS_N286


title: Ruvseq_Patient 284 CBCS_N284


title: Ruvseq_Patient 285 CBCS_N285


title: Ruvseq_Patient 283 CBCS_N283


title: Ruvseq_Patient 282 CBCS_N282


title: S17202_M7_Emx_Cx_P0_mat


title: Ruvseq_Patient 281 CBCS_N281


title: S17201_M7_Emx_Cx_P0_mat


title: S17200_M7_Emx_Cx_P0_mat


title: Ruvseq_Patient 279 CBCS_N279


title: S17199_M7_Emx_Cx_P0_mat


title: S17198_M7_Emx_Cx_P0_mat


title: S17197_M7_Emx_Cx_P0_mat


title: Ruvseq_Patient 276 CBCS_N276


title: Ruvseq_Patient 275 CBCS_N275


title: Ruvseq_Patient 278 CBCS_N278


title: S17196_M7_Emx_Cx_P0_mat


title: S17195_M7_Emx_Cx_P0_mat


title: Ruvseq_Patient 274 CBCS_N274


title: S17194_M7_Emx_Cx_P0_mat


title: S17193_M7_Emx_Cx_P0_mat


title: S17192_M7_Emx_Cx_P0_mat


title: Ruvseq_Patient 280 CBCS_N280


title: Ruvseq_Patient 273 CBCS_N273


title: S17188_M7_Emx_Cx_P0_mat


title: S17191_M7_Emx_Cx_P0_mat


title: Ruvseq_Patient 271 CBCS_N271


title: S17189_M7_Emx_Cx_P0_mat


title: Ruvseq_Patient 272 CBCS_N272


title: Ruvseq_Patient 269 CBCS_N269


title: Ruvseq_Patient 267 CBCS_N267


title: Ruvseq_Patient 270 CBCS_N270


title: Ruvseq_Patient 277 CBCS_N277


title: Ruvseq_Patient 266 CBCS_N266


title: S17187_M7_Emx_Cx_P0_mat


title: S17190_M7_Emx_Cx_P0_mat


title: Ruvseq_Patient 264 CBCS_N264


title: Ruvseq_Patient 265 CBCS_N265


title: Ruvseq_Patient 263 CBCS_N263


title: Ruvseq_Patient 262 CBCS_N262


title: Ruvseq_Patient 268 CBCS_N268


title: Ruvseq_Patient 259 CBCS_N259


title: Ruvseq_Patient 260 CBCS_N260


title: 82442: M7_Emx_OB_patUPD

data_processing:
Raw reads were delivered as BAM files and converted to fastq format using bamToFastq (bedtools suite v2.26.0) for alignment. Reads within transcripts were counted using STAR_2.5.0c with an index prepared from GRCm38 and Gencode M16 gene annotation. Note that for optimal representation of the long non-coding RNA Meg3 the spliced ENSMUST00000143836.7 transcript was modified to represent a single exon transcript. Parameters used for STAR alignment: --outFilterMultimapNmax 1 --clip5pNbases 12 --quantMode GeneCounts
Differential expression between all possible pairwise comparisons of UPD states in all cell types was calculated using DESeq2 v1.16.1. Results are given as supplementary file.
Genome_build: mm10/GRCm38
Supplementary_files_format_and_content: Read counts in transcripts as reported by STAR as tab delimited text
Supplementary_files_format_and_content: Excel spreadsheet with combined differential expression analysis as reported by DESeq2. One tab per cell type.

growth_protocol: All mouse colonies were maintained in accordance with protocols approved by institutional animal care and use committee, institutional ethics committee and the preclinical core facility (PCF) at IST Austria. Experiments were performed under a license approved by the Austrian Federal Ministry of Science and Research in accordance with the Austrian and EU animal laws. Mouse lines used: MADM-7-GT (JAX stock # 021457), MADM-7-TG (JAX stock # 021458), Emx1-Cre (JAX stock # 005628), Nkx2.1-Cre (JAX stock # 008661)

extract_protocol:
Samples in isolation Buffer (IB; Lexogen) were processed according to the SPLIT kit instructions (Lexogen). All other samples were incubated for 30min at 37°C. Total volume was filled to 250µl using RNase-free H2O (Thermo Fisher Scientific) followed by addition of 750µl Trizol LS (Thermo Fisher Scientific). Samples were mixed by 5 times inverting. After a 5min incubation step at RT, the entire solution was transferred into a MaXtract tube (Qiagen). 200µl chloroform (Sigma-Aldrich) was added, followed by 3 times 5sec vortexing and 2min incubation at RT. Samples were centrifuged for 2min with 12000rpm at 18°C. Supernatant was transferred to a new tube and isopropanol (Sigma-Aldrich) was added in a 1:1 ratio. For better visibility of the RNA pellet 1µl GlycoBlue (Thermo Fisher Scientific) was added and entire solution was mixed by vortexing (3x 5sec). Samples were left for precipitation o/n at -20°C. After precipitation samples were centrifuged for 20min with 14000rpm at 4°C. Supernatant was removed and RNA pellet was washed with 70% ethanol, followed by a 5min centrifugation step (14000rpm at 4°C). RNA pellet was resuspended in an appropriate amount of RNase-free H2O. RNA quality was analyzed using Bioanalyzer RNA 6000 Nano (Agilent) and RNA 6000 Pico kit (Agilent) following the manufacturer’s instructions.
SMART-Seq2 libraries were prepared from purified RNA (VBC GmbH, Vienna)

treatment_protocol: P0 animals were sacrificed and brain areas of interest (neocortex, hippocampus and olfactory bulb) were dissected. Single cell suspensions were prepared by using Papain containing L-cysteine and EDTA (vial 2, Worthington), DNase I (vial 3, Worthington), Ovomucoid protease inhibitor (vial 4, Worthington), EBSS (Thermo Fisher Scientific), DMEM/F12 (Thermo Fisher Scientific), FBS and HS. All vials from Worthington kit were reconstituted according to the manufacturer’s instructions using EBSS. The dissected brain areas were directly placed into Papain-DNase solution (20units/ml papain and 1000 units DNase). Entire litters were pooled in order to obtain sufficient material for RNA extraction. Enzymatic digestion was performed for 30min at 37°C in a shaking water bath. Next, solution 2 (EBSS containing 0.67mg Ovomucoid protease inhibitor and 166.7 U/ml DNase I) was added, the whole suspension was thoroughly mixed and centrifuged for 5min at 1000rpm at RT. Supernatant was removed and cell pellet was resuspended in solution 2. Trituration with p1000 pipette was performed to mechanically dissolve any remaining tissue parts. DMEM/F12 was added to the cell suspension as a washing solution, followed by a centrifugation step of 5min with 1500rpm at RT. Cells were resuspended in media (DMEM/F12 containing 10% FBS and 10% HS) and kept on ice until sorted. Right before sorting, cell suspension was filtered using a 40µm cell strainer. FACS was performed on a BD FACS Aria III using 100 nozzle and keeping sample and collection devices at 4°C. Duplet exclusion was performed to ensure sorting of true single cells. GFP+ (green), tdTomato+ (red), and GFP+/tdTomato+ (yellow) cells were collected. Cells were sorted directly into either Isolation Buffer (IB; Lexogen) if more than 2000 sorted cells/per color were expected or if samples yielded fewer labeled cells into custom made lysis buffer (30nM TRIS pH 8, 10nM EDTA pH 8, 1% SDS and 200 µg/µL Proteinase K).

value: Olfactory bulb

value: red


title: 82456: M7_Emx_Hi_matUPD

value: Emx1 lineage

value: Hippocampus


title: 82447: M7_Emx_OB_wt

value: yellow

value: wt


title: 82445: M7_Emx_OB_matUPD


title: 82454: M7_Emx_Cx_matUPD

value: Neocortex


title: 82455: M7_Emx_Cx_patUPD

value: green


title: 82444: M7_Emx_OB_wt


title: 82443: M7_Emx_OB_matUPD


title: 82440: M7_Emx_OB_matUPD


title: Ruvseq_Patient 254 CBCS_N254

value: LumA


title: Ruvseq_Patient 256 CBCS_N256

value: primary breast tumor


title: 82438: M7_Emx_OB_wt


title: 82439: M7_Emx_OB_patUPD


title: Ruvseq_Patient 257 CBCS_N257

value: LumB


title: Ruvseq_Patient 252 CBCS_N252

value: Normal


title: Ruvseq_Patient 253 CBCS_N253


title: Ruvseq_Patient 251 CBCS_N251


title: 82437: M7_Emx_OB_patUPD

value: pat


title: 82446: M7_Emx_OB_patUPD


title: 82436: M7_Emx_OB_matUPD

value: mat


title: Ruvseq_Patient 250 CBCS_N250


title: 82441: M7_Emx_OB_wt


title: Ruvseq_Patient 261 CBCS_N261


title: Ruvseq_Patient 258 CBCS_N258


title: 82435: M7_Emx_OB_wt


title: 82434: M7_Emx_OB_patUPD


title: Ruvseq_Patient 255 CBCS_N255


title: Ruvseq_Patient 248 CBCS_N248


title: Ruvseq_Patient 249 CBCS_N249


title: 82432: M7_Nkx_Hi_wt

value: Nkx2.1 lineage


title: 82433: M7_Emx_OB_matUPD


title: 82430: M7_Nkx_Hi_matUPD


title: 82431: M7_Nkx_Hi_patUPD


title: 82428: M7_Nkx_Hi_patUPD


title: 82429: M7_Nkx_Hi_wt


title: Ruvseq_Patient 245 CBCS_N245

value: Positive


title: Ruvseq_Patient 244 CBCS_N244


title: 82424: M7_Nkx_Hi_patUPD


title: Ruvseq_Patient 246 CBCS_N246


title: 82425: M7_Nkx_Hi_matUPD


title: Ruvseq_Patient 247 CBCS_N247


title: 82426: M7_Nkx_Hi_wt


title: Ruvseq_Patient 243 CBCS_N243


title: Ruvseq_Patient 238 CBCS_N238


title: Ruvseq_Patient 237 CBCS_N237


title: Ruvseq_Patient 240 CBCS_N240


title: Ruvseq_Patient 241 CBCS_N241


title: Ruvseq_Patient 239 CBCS_N239


title: Ruvseq_Patient 236 CBCS_N236


title: 82423: M7_Nkx_Hi_wt


title: 82422: M7_Nkx_Hi_matUPD


title: Ruvseq_Patient 242 CBCS_N242


title: Ruvseq_Patient 235 CBCS_N235


title: 82421: M7_Nkx_Hi_patUPD


title: Ruvseq_Patient 234 CBCS_N234

value: Basal


title: Ruvseq_Patient 233 CBCS_N233


title: 82427: M7_Nkx_Hi_matUPD


title: 82418: M7_Nkx_Cx_matUPD


title: Ruvseq_Patient 232 CBCS_N232


title: 82416: M7_Nkx_Cx_matUPD


title: Ruvseq_Patient 230 CBCS_N230


title: Ruvseq_Patient 231 CBCS_N231


title: 82414: M7_Nkx_Cx_wt


title: Ruvseq_Patient 229 CBCS_N229


title: 82415: M7_Nkx_Cx_patUPD


title: 82412: M7_Nkx_Cx_matUPD


title: Ruvseq_Patient 226 CBCS_N226


title: Ruvseq_Patient 228 CBCS_N228


title: 82413: M7_Nkx_Cx_patUPD


title: 82410: M7_Nkx_Cx_matUPD


title: 82411: M7_Nkx_Cx_wt


title: Ruvseq_Patient 227 CBCS_N227


title: 82409: M7_Nkx_Cx_patUPD


title: 82420: M7_Nkx_Cx_wt


title: Ruvseq_Patient 222 CBCS_N222


title: Ruvseq_Patient 225 CBCS_N225


title: Ruvseq_Patient 223 CBCS_N223


title: Ruvseq_Patient 224 CBCS_N224


title: 82407: M7_Emx_Hi_matUPD


title: 82419: M7_Nkx_Cx_patUPD


title: 82417: M7_Nkx_Cx_wt


title: Ruvseq_Patient 221 CBCS_N221


title: Ruvseq_Patient 220 CBCS_N220


title: 82406: M7_Emx_Hi_patUPD


title: Ruvseq_Patient 219 CBCS_N219


title: 82405: M7_Emx_Hi_wt


title: 82404: M7_Emx_Hi_matUPD


title: Ruvseq_Patient 218 CBCS_N218


title: 82403: M7_Emx_Hi_patUPD


title: 82402: M7_Emx_Hi_wt


title: 82400: M7_Emx_Hi_matUPD


title: 82401: M7_Emx_Hi_patUPD


title: 82408: M7_Emx_Hi_wt


title: 82398: M7_Emx_Hi_patUPD


title: Ruvseq_Patient 216 CBCS_N216


title: 82397: M7_Emx_Hi_matUPD


title: Ruvseq_Patient 215 CBCS_N215


title: 82399: M7_Emx_Hi_wt


title: 82395: M7_Emx_Cx_matUPD


title: 82394: M7_Emx_Cx_patUPD


title: Ruvseq_Patient 213 CBCS_N213


title: Ruvseq_Patient 214 CBCS_N214


title: Ruvseq_Patient 212 CBCS_N212


title: Ruvseq_Patient 210 CBCS_N210


title: Ruvseq_Patient 209 CBCS_N209


title: Ruvseq_Patient 211 CBCS_N211


title: Ruvseq_Patient 208 CBCS_N208


title: 82392: M7_Emx_Cx_matUPD


title: 82393: M7_Emx_Cx_wt


title: Ruvseq_Patient 207 CBCS_N207


title: Ruvseq_Patient 205 CBCS_N205


title: 82391: M7_Emx_Cx_patUPD


title: 82390: M7_Emx_Cx_wt


title: Ruvseq_Patient 217 CBCS_N217


title: Ruvseq_Patient 204 CBCS_N204


title: 82389: M7_Emx_Cx_patUPD


title: 82388: M7_Emx_Cx_matUPD


title: 82396: M7_Emx_Cx_wt


title: Ruvseq_Patient 203 CBCS_N203


title: Ruvseq_Patient 206 CBCS_N206


title: 82386: M7_Emx_Cx_patUPD


title: Ruvseq_Patient 202 CBCS_N202


title: Ruvseq_Patient 201 CBCS_N201


title: Ruvseq_Patient 200 CBCS_N200


title: Ruvseq_Patient 198 CBCS_N198


title: Ruvseq_Patient 196 CBCS_N196


title: Ruvseq_Patient 197 CBCS_N197


title: Ruvseq_Patient 195 CBCS_N195


title: S17425_M7_Emx_Cx_P0_wt


title: CB_73018

data_processing:
Raw reads were delivered as BAM files and converted to fastq format using bamToFastq (bedtools suite v2.26.0) for alignment. Reads within transcripts were counted using STAR_2.5.0c with an index prepared from GRCm38 and Gencode M16 gene annotation. Note that for optimal representation of the long non-coding RNA Meg3 the spliced ENSMUST00000143836.7 transcript was modified to represent a single exon transcript. Parameters used for STAR alignment: --outFilterMultimapNmax 1 --outSAMstrandField intronMotif --outFilterIntronMotifs RemoveNoncanonical --outSAMtype BAM SortedByCoordinate --quantMode GeneCounts
FPKM values were calculated from BAM files using cufflinks v2.2.1. Note that cells with <1M reads were excluded from this analysis.
Genome_build: mm10/GRCm38
Supplementary_files_format_and_content: Read counts in transcripts as reported by STAR as tab delimited text

growth_protocol: All mouse colonies were maintained in accordance with protocols approved by institutional animal care and use committee, institutional ethics committee and the preclinical core facility (PCF) at IST Austria. Experiments were performed under a license approved by the Austrian Federal Ministry of Science and Research in accordance with the Austrian and EU animal laws. Mouse lines used: Emx1-Cre REF (JAX stock # 005628); Z/EG (JAX stock # 004178); CAST/EiJ (JAX stock # 000928); C57Bl/6J (JAX stock # 000664)

extract_protocol:
Single cells were sorted into 96 well plates (Bio-Rad) containing 4µl lysis buffer (0.2% Triton X-100, 2U/µl RNase Inhibitor [Clonetech]) per well.
sorted cells were directly processed without RNA extraction using  Smart-Seq2 (VBCF GmbH Vienna)

treatment_protocol: Animals were sacrificed and brain areas of interest were dissected. Single cell suspensions were prepared by using Papain containing L-cysteine and EDTA (vial 2, Worthington), DNase I (vial 3, Worthington), Ovomucoid protease inhibitor (vial 4, Worthington), EBSS (Thermo Fisher Scientific), DMEM/F12 (Thermo Fisher Scientific), FBS and HS. All vials from Worthington kit were reconstituted according to the manufacturer’s instructions using EBSS. The dissected brain areas were directly placed into Papain-DNase solution (20units/ml papain and 1000 units DNase). Individual animals were used for each experiment. Enzymatic digestion was performed for 30min at 37°C in a shaking water bath. Next, solution 2 (EBSS containing 0.67mg Ovomucoid protease inhibitor and 166.7 U/ml DNase I) was added, the whole suspension was thoroughly mixed and centrifuged for 5min at 1000rpm at RT. Supernatant was removed and cell pellet was resuspended in solution 2. Trituration with p1000 pipette was performed to mechanically dissolve any remaining tissue parts. DMEM/F12 was added to the cell suspension as a washing solution, followed by a centrifugation step of 5min with 1500rpm at RT. Cells were resuspended in media (DMEM/F12 containing 10% FBS and 10% HS) and kept on ice until sorted. Right before sorting, cell suspension was filtered using a 40µm cell strainer. FACS was performed on a BD FACS Aria III using 100 nozzle and keeping sample and collection devices at 4°C. Duplet exclusion was performed to ensure sorting of true single cells. GFP+ (green) cells were collected.

value: Cast

tag: Sex

value: female


title: CB_73017

value: Emx1 lineage


title: CB_73019


title: CB_73016


title: Ruvseq_Patient 194 CBCS_N194


title: CB_73015


title: CB_73014


title: Ruvseq_Patient 193 CBCS_N193


title: CB_73020


title: 82387: M7_Emx_Cx_wt


title: CB_73013


title: Ruvseq_Patient 192 CBCS_N192


title: CB_73012


title: CB_73011


title: CB_73010


title: Ruvseq_Patient 191 CBCS_N191


title: 82385: M7_Emx_Cx_matUPD

value: red


title: Ruvseq_Patient 190 CBCS_N190


title: Ruvseq_Patient 199 CBCS_N199


title: Ruvseq_Patient 189 CBCS_N189


title: CB_73008


title: CB_73009


title: Ruvseq_Patient 188 CBCS_N188


title: Ruvseq_Patient 187 CBCS_N187


title: Ruvseq_Patient 186 CBCS_N186


title: Ruvseq_Patient 185 CBCS_N185


title: CB_73007


title: CB_73005


title: CB_73004


title: CB_73002


title: CB_73001


title: Ruvseq_Patient 183 CBCS_N183


title: Ruvseq_Patient 181 CBCS_N181


title: Ruvseq_Patient 184 CBCS_N184


title: Ruvseq_Patient 182 CBCS_N182


title: CB_72998


title: CB_72999


title: CB_73006


title: Ruvseq_Patient 178 CBCS_N178


title: CB_73000


title: Ruvseq_Patient 177 CBCS_N177


title: CB_72994


title: CB_72996


title: CB_72995


title: CB_72997


title: Ruvseq_Patient 175 CBCS_N175


title: Ruvseq_Patient 174 CBCS_N174


title: Ruvseq_Patient 179 CBCS_N179


title: CB_72993


title: Ruvseq_Patient 173 CBCS_N173


title: Ruvseq_Patient 172 CBCS_N172


title: Ruvseq_Patient 176 CBCS_N176


title: Ruvseq_Patient 171 CBCS_N171


title: CB_73003


title: CB_72991


title: Ruvseq_Patient 170 CBCS_N170


title: Ruvseq_Patient 169 CBCS_N169

value: Negative


title: CB_72989


title: CB_72990


title: CB_72987


title: CB_72986


title: Ruvseq_Patient 168 CBCS_N168


title: Ruvseq_Patient 166 CBCS_N166


title: CB_72985


title: Ruvseq_Patient 167 CBCS_N167


title: Ruvseq_Patient 164 CBCS_N164


title: Ruvseq_Patient 163 CBCS_N163


title: Ruvseq_Patient 165 CBCS_N165


title: CB_72992


title: CB_72983


title: CB_72979


title: CB_72981


title: Ruvseq_Patient 162 CBCS_N162


title: Ruvseq_Patient 159 CBCS_N159


title: Ruvseq_Patient 156 CBCS_N156


title: Ruvseq_Patient 180 CBCS_N180


title: CB_72978


title: CB_72984


title: Ruvseq_Patient 160 CBCS_N160


title: CB_72977


title: Ruvseq_Patient 155 CBCS_N155


title: Ruvseq_Patient 158 CBCS_N158


title: CB_72976


title: CB_72975


title: Ruvseq_Patient 157 CBCS_N157


title: CB_72973


title: CB_72982


title: Ruvseq_Patient 153 CBCS_N153


title: CB_72980


title: Ruvseq_Patient 151 CBCS_N151


title: CB_72988


title: Ruvseq_Patient 150 CBCS_N150


title: Ruvseq_Patient 161 CBCS_N161


title: CB_72972


title: CB_72971


title: CB_72970


title: Ruvseq_Patient 149 CBCS_N149


title: Ruvseq_Patient 147 CBCS_N147


title: Ruvseq_Patient 148 CBCS_N148


title: CB_72968


title: Ruvseq_Patient 146 CBCS_N146


title: Ruvseq_Patient 145 CBCS_N145


title: CB_72974


title: CB_72965


title: CB_72966


title: Ruvseq_Patient 144 CBCS_N144


title: Ruvseq_Patient 154 CBCS_N154


title: CB_72964


title: Ruvseq_Patient 152 CBCS_N152


title: Ruvseq_Patient 141 CBCS_N141


title: Ruvseq_Patient 142 CBCS_N142


title: CB_72962


title: Ruvseq_Patient 143 CBCS_N143


title: CB_72963


title: Ruvseq_Patient 140 CBCS_N140


title: Ruvseq_Patient 139 CBCS_N139


title: CB_72969


title: CB_72961


title: CB_72958


title: CB_72959


title: CB_72967


title: Ruvseq_Patient 138 CBCS_N138


title: Ruvseq_Patient 136 CBCS_N136


title: CB_72957


title: Ruvseq_Patient 137 CBCS_N137


title: CB_72956


title: CB_72955


title: CB_72954


title: CB_72953


title: CB_72952


title: Ruvseq_Patient 135 CBCS_N135


title: CB_72951


title: Ruvseq_Patient 132 CBCS_N132


title: Ruvseq_Patient 133 CBCS_N133


title: Ruvseq_Patient 134 CBCS_N134


title: CB_72960


title: CB_72950


title: Ruvseq_Patient 131 CBCS_N131


title: Ruvseq_Patient 128 CBCS_N128


title: Ruvseq_Patient 129 CBCS_N129


title: Ruvseq_Patient 130 CBCS_N130


title: CB_72948


title: CB_72947


title: Ruvseq_Patient 127 CBCS_N127


title: CB_72949


title: CB_72945


title: CB_72946


title: Ruvseq_Patient 126 CBCS_N126


title: CB_72944


title: Ruvseq_Patient 124 CBCS_N124


title: Ruvseq_Patient 123 CBCS_N123


title: Ruvseq_Patient 121 CBCS_N121


title: Ruvseq_Patient 122 CBCS_N122


title: CB_72943


title: CB_72942


title: CB_72941


title: Ruvseq_Patient 120 CBCS_N120


title: Ruvseq_Patient 119 CBCS_N119


title: Ruvseq_Patient 118 CBCS_N118


title: Ruvseq_Patient 117 CBCS_N117


title: CB_72939


title: CB_72940


title: Ruvseq_Patient 115 CBCS_N115


title: Ruvseq_Patient 116 CBCS_N116


title: CB_72937


title: CB_72936


title: CB_72935


title: Ruvseq_Patient 125 CBCS_N125


title: CB_72938


title: CB_72933


title: CB_72932


title: CB_72934


title: Ruvseq_Patient 113 CBCS_N113


title: Ruvseq_Patient 111 CBCS_N111


title: Ruvseq_Patient 112 CBCS_N112


title: Ruvseq_Patient 110 CBCS_N110


title: CB_72930


title: CB_72931


title: CB_72929


title: Ruvseq_Patient 108 CBCS_N108


title: Ruvseq_Patient 107 CBCS_N107


title: Ruvseq_Patient 109 CBCS_N109


title: Ruvseq_Patient 106 CBCS_N106


title: Ruvseq_Patient 114 CBCS_N114


title: BC_72915

value: male


title: BC_72914

value: Neocortex


title: Ruvseq_Patient 103 CBCS_N103


title: BC_72913


title: BC_72912


title: Ruvseq_Patient 104 CBCS_N104


title: Ruvseq_Patient 102 CBCS_N102


title: BC_72910


title: BC_72909


title: Ruvseq_Patient 99 CBCS_N99


title: Ruvseq_Patient 101 CBCS_N101


title: Ruvseq_Patient 100 CBCS_N100


title: BC_72908


title: BC_72906


title: BC_72907


title: BC_72905


title: Ruvseq_Patient 97 CBCS_N97


title: Ruvseq_Patient 96 CBCS_N96


title: CB_72928


title: BC_72904


title: Ruvseq_Patient 95 CBCS_N95


title: Ruvseq_Patient 94 CBCS_N94


title: Ruvseq_Patient 105 CBCS_N105


title: BC_72911


title: Ruvseq_Patient 93 CBCS_N93


title: Ruvseq_Patient 92 CBCS_N92


title: Ruvseq_Patient 98 CBCS_N98


title: Ruvseq_Patient 88 CBCS_N88


title: Ruvseq_Patient 89 CBCS_N89


title: Ruvseq_Patient 90 CBCS_N90


title: BC_72898


title: BC_72897


title: BC_72900


title: BC_72901


title: BC_72896


title: BC_72895


title: Ruvseq_Patient 91 CBCS_N91


title: BC_72894


title: BC_72899


title: Ruvseq_Patient 87 CBCS_N87


title: Ruvseq_Patient 86 CBCS_N86


title: BC_72893


title: BC_72903


title: Ruvseq_Patient 84 CBCS_N84


title: Ruvseq_Patient 83 CBCS_N83


title: BC_72902


title: Ruvseq_Patient 82 CBCS_N82


title: Ruvseq_Patient 85 CBCS_N85


title: Ruvseq_Patient 80 CBCS_N80


title: BC_72890


title: BC_72892


title: BC_72891


title: Ruvseq_Patient 79 CBCS_N79


title: BC_72889


title: BC_72887


title: BC_72888


title: BC_72884


title: Ruvseq_Patient 78 CBCS_N78


title: BC_72885


title: Ruvseq_Patient 77 CBCS_N77


title: Ruvseq_Patient 76 CBCS_N76


title: BC_72882


title: BC_72881


title: Ruvseq_Patient 74 CBCS_N74


title: Ruvseq_Patient 75 CBCS_N75


title: BC_72880


title: Ruvseq_Patient 81 CBCS_N81


title: BC_72883


title: Ruvseq_Patient 73 CBCS_N73


title: BC_72878


title: BC_72879


title: BC_72877


title: Ruvseq_Patient 71 CBCS_N71


title: Ruvseq_Patient 68 CBCS_N68


title: BC_72875


title: Ruvseq_Patient 69 CBCS_N69


title: BC_72874


title: Ruvseq_Patient 70 CBCS_N70


title: Ruvseq_Patient 67 CBCS_N67


title: Ruvseq_Patient 64 CBCS_N64


title: BC_72876


title: BC_72886


title: Ruvseq_Patient 65 CBCS_N65


title: Ruvseq_Patient 66 CBCS_N66


title: BC_72873


title: BC_72872


title: Ruvseq_Patient 63 CBCS_N63


title: BC_72871


title: Ruvseq_Patient 72 CBCS_N72


title: Ruvseq_Patient 62 CBCS_N62


title: Ruvseq_Patient 61 CBCS_N61


title: BC_72868


title: BC_72870


title: Ruvseq_Patient 60 CBCS_N60


title: Ruvseq_Patient 59 CBCS_N59


title: BC_72867


title: BC_72866


title: Ruvseq_Patient 58 CBCS_N58


title: Ruvseq_Patient 57 CBCS_N57


title: BC_72865


title: Ruvseq_Patient 56 CBCS_N56


title: BC_72864


title: Ruvseq_Patient 53 CBCS_N53


title: BC_72862


title: BC_72861


title: Ruvseq_Patient 52 CBCS_N52


title: Ruvseq_Patient 54 CBCS_N54


title: Ruvseq_Patient 51 CBCS_N51


title: BC_72859


title: BC_72858


title: BC_72860


title: Ruvseq_Patient 50 CBCS_N50

value: Weak Positive / Borderline


title: BC_72857


title: BC_72869


title: Ruvseq_Patient 49 CBCS_N49

value: Unknown

value: LumA


title: Ruvseq_Patient 47 CBCS_N47

value: primary breast tumor


title: Ruvseq_Patient 48 CBCS_N48


title: Ruvseq_Patient 46 CBCS_N46

value: Normal


title: BC_72855


title: BC_72854


title: BC_72856


title: Ruvseq_Patient 55 CBCS_N55


title: Ruvseq_Patient 45 CBCS_N45


title: BC_72863


title: BC_72853


title: Ruvseq_Patient 44 CBCS_N44


title: Ruvseq_Patient 43 CBCS_N43

value: LumB


title: Ruvseq_Patient 42 CBCS_N42


title: BC_72852


title: BC_72851


title: Ruvseq_Patient 41 CBCS_N41


title: Ruvseq_Patient 40 CBCS_N40


title: Ruvseq_Patient 38 CBCS_N38


title: BC_72848


title: BC_72849


title: BC_72846


title: BC_72847


title: BC_72845


title: Ruvseq_Patient 37 CBCS_N37


title: Ruvseq_Patient 36 CBCS_N36


title: Ruvseq_Patient 34 CBCS_N34


title: BC_72843


title: Ruvseq_Patient 35 CBCS_N35


title: BC_72844


title: BC_72842


title: BC_72841


title: Ruvseq_Patient 33 CBCS_N33


title: BC_72839


title: BC_72840


title: Ruvseq_Patient 30 CBCS_N30


title: BC_72850


title: BC_72837


title: BC_72838


title: Ruvseq_Patient 39 CBCS_N39


title: Ruvseq_Patient 29 CBCS_N29


title: Ruvseq_Patient 27 CBCS_N27


title: Ruvseq_Patient 28 CBCS_N28


title: Ruvseq_Patient 25 CBCS_N25


title: Ruvseq_Patient 26 CBCS_N26


title: BC_72834


title: BC_72835


title: BC_72832


title: Ruvseq_Patient 23 CBCS_N23


title: Ruvseq_Patient 24 CBCS_N24


title: Ruvseq_Patient 32 CBCS_N32


title: Ruvseq_Patient 31 CBCS_N31


title: Ruvseq_Patient 22 CBCS_N22


title: BC_72830


title: Ruvseq_Patient 18 CBCS_N18


title: Ruvseq_Patient 21 CBCS_N21


title: Ruvseq_Patient 19 CBCS_N19


title: BC_72831


title: BC_72829


title: BC_72828


title: BC_72836


title: Ruvseq_Patient 20 CBCS_N20


title: BC_72827


title: BC_72826


title: BC_72824


title: BC_72833


title: Ruvseq_Patient 17 CBCS_N17


title: Ruvseq_Patient 15 CBCS_N15


title: BC_72823


title: BC_72719


title: Ruvseq_Patient 16 CBCS_N16


title: Ruvseq_Patient 14 CBCS_N14


title: Ruvseq_Patient 13 CBCS_N13


title: BC_72718


title: Ruvseq_Patient 11 CBCS_N11


title: Ruvseq_Patient 12 CBCS_N12


title: Ruvseq_Patient 10 CBCS_N10


title: BC_72717


title: BC_72715


title: Ruvseq_Patient 9 CBCS_N9


title: BC_72716


title: Ruvseq_Patient 8 CBCS_N8


title: BC_72714


title: BC_72712


title: BC_72711


title: BC_72713


title: Ruvseq_Patient 7 CBCS_N7


title: Ruvseq_Patient 5 CBCS_N5


title: BC_72709


title: Ruvseq_Patient 4 CBCS_N4


title: BC_72825


title: BC_72707


title: BC_72706


title: Ruvseq_Patient 2 CBCS_N2


title: BC_72705


title: Ruvseq_Patient 1 CBCS_N1


title: Ruvseq_Patient 6 CBCS_N6


title: BC_72710


title: Ruvseq_Patient 3 CBCS_N3


title: Patient 1294 CBCS_N1294

data_processing: The raw data were scaled with the geometric mean of 6 positive control genes (provided on standard NanoString nCounter chips) and geometric mean of 11 housekeeping genes (labelled as Housekeeping genes in raw data). Details provided: https://www.nanostring.com/download_file/view/1168/3779


title: BC_72704


title: Patient 1292 CBCS_N1292


title: Patient 1291 CBCS_N1291


title: BC_72702


title: Patient 1290 CBCS_N1290


title: BC_72701


title: Patient 1289 CBCS_N1289


title: Patient 1288 CBCS_N1288


title: BC_72699


title: BC_72708


title: BC_72698


title: Patient 1286 CBCS_N1286


title: Patient 1287 CBCS_N1287


title: BC_72697


title: BC_72696


title: Patient 1293 CBCS_N1293


title: Patient 1285 CBCS_N1285


title: BC_72695


title: BC_72694


title: Patient 1284 CBCS_N1284


title: Patient 1281 CBCS_N1281


title: BC_72703


title: Patient 1282 CBCS_N1282


title: BC_72691


title: Patient 1280 CBCS_N1280


title: BC_72693


title: Patient 1278 CBCS_N1278


title: BC_72700


title: Patient 1279 CBCS_N1279


title: Patient 1277 CBCS_N1277


title: BC_72689


title: Patient 1276 CBCS_N1276


title: Patient 1275 CBCS_N1275


title: BC_72687


title: Patient 1274 CBCS_N1274


title: Patient 1283 CBCS_N1283


title: BC_72686


title: Patient 1273 CBCS_N1273


title: Patient 1272 CBCS_N1272


title: BC_72685


title: BC_72684


title: BC_72683


title: BC_72692


title: Patient 1271 CBCS_N1271


title: Patient 1270 CBCS_N1270


title: BC_72690


title: Patient 1269 CBCS_N1269


title: BC_72682


title: Patient 1267 CBCS_N1267


title: BC_72681


title: BC_72679


title: Patient 1268 CBCS_N1268


title: Patient 1266 CBCS_N1266


title: BC_72678


title: Patient 1265 CBCS_N1265


title: BC_72677


title: BC_72688


title: BC_72676


title: Patient 1264 CBCS_N1264


title: BC_72673


title: BC_72674


title: Patient 1263 CBCS_N1263


title: Patient 1262 CBCS_N1262


title: BC_72675


title: Patient 1261 CBCS_N1261


title: Patient 1260 CBCS_N1260


title: Patient 1259 CBCS_N1259


title: BC_72671


title: BC_72680


title: BC_72670


title: Patient 1258 CBCS_N1258


title: BC_72668


title: BC_72669


title: Patient 1257 CBCS_N1257


title: Patient 1256 CBCS_N1256


title: Patient 1254 CBCS_N1254


title: Patient 1255 CBCS_N1255


title: BC_72666


title: BC_72664


title: BC_72665


title: BC_72663


title: Patient 1219 CBCS_N1219


title: BC_72667


title: BC_72662


title: Patient 1218 CBCS_N1218


title: BC_72672


title: BC_72661


title: Patient 1217 CBCS_N1217


title: BC_72659


title: BC_72660


title: Patient 1216 CBCS_N1216


title: Patient 1215 CBCS_N1215


title: BC_72657


title: Patient 1214 CBCS_N1214


title: BC_72658


title: Patient 1213 CBCS_N1213


title: Patient 1212 CBCS_N1212


title: Patient 1211 CBCS_N1211


title: Patient 1210 CBCS_N1210


title: BC_72656


title: BC_72655


title: BC_72654


title: Patient 1209 CBCS_N1209


title: Patient 1208 CBCS_N1208


title: BC_72653


title: BC_72652


title: BC_72651


title: BC_72650


title: Patient 1206 CBCS_N1206


title: Patient 1207 CBCS_N1207


title: Patient 1205 CBCS_N1205


title: BC_72649


title: BC_72648


title: Patient 1190 CBCS_N1190


title: BC_72647


title: BC_72646


title: Patient 1191 CBCS_N1191


title: BC_72645


title: BC_72642


title: Patient 1203 CBCS_N1203


title: Patient 1187 CBCS_N1187


title: BC_72643


title: BC_72641


title: BC_72644


title: Patient 1201 CBCS_N1201


title: Patient 1200 CBCS_N1200


title: Patient 1253 CBCS_N1253


title: Patient 1204 CBCS_N1204


title: Patient 1199 CBCS_N1199


title: BC_72640


title: BC_72638


title: Patient 1189 CBCS_N1189


title: BC_72639


title: BC_72636


title: Patient 1185 CBCS_N1185


title: Patient 1188 CBCS_N1188


title: Patient 1186 CBCS_N1186


title: BC_72634


title: Patient 1202 CBCS_N1202


title: Patient 1196 CBCS_N1196


title: BC_72633


title: BC_72631


title: BC_72632


title: Patient 1194 CBCS_N1194


title: Patient 1195 CBCS_N1195


title: BC_72630


title: Patient 1193 CBCS_N1193


title: Patient 1192 CBCS_N1192


title: Patient 1252 CBCS_N1252


title: BC_72629


title: BC_72628


title: Patient 1198 CBCS_N1198


title: Patient 752 CBCS_N752


title: Patient 751 CBCS_N751


title: Patient 1197 CBCS_N1197


title: BC_72637


title: BC_72635


title: Patient 750 CBCS_N750


title: Patient 749 CBCS_N749


title: CB_72575


title: CB_72573


title: CB_72574


title: CB_72572


title: BC_72627


title: Patient 748 CBCS_N748


title: Patient 829 CBCS_N829


title: Patient 830 CBCS_N830


title: Patient 746 CBCS_N746


title: CB_72571


title: Patient 747 CBCS_N747


title: CB_72569


title: CB_72568


title: CB_72567


title: CB_72570


title: CB_72566


title: CB_72565


title: Patient 827 CBCS_N827


title: CB_72563


title: CB_72564


title: Patient 743 CBCS_N743


title: Patient 744 CBCS_N744


title: CB_72560


title: CB_72559


title: CB_72562


title: CB_72561


title: Patient 740 CBCS_N740


title: CB_72558


title: Patient 824 CBCS_N824


title: CB_72556


title: Patient 741 CBCS_N741


title: Patient 822 CBCS_N822


title: CB_72557


title: Patient 742 CBCS_N742


title: Patient 821 CBCS_N821


title: CB_72554


title: Patient 828 CBCS_N828


title: Patient 739 CBCS_N739


title: CB_72555


title: Patient 819 CBCS_N819


title: Patient 823 CBCS_N823


title: Patient 826 CBCS_N826


title: CB_72552


title: Patient 745 CBCS_N745


title: Patient 825 CBCS_N825


title: CB_72551


title: CB_72553


title: Patient 738 CBCS_N738


title: Patient 820 CBCS_N820


title: CB_72550


title: CB_72548


title: Patient 735 CBCS_N735


title: Patient 737 CBCS_N737


title: Patient 736 CBCS_N736


title: CB_72545


title: CB_72546


title: Patient 734 CBCS_N734


title: Patient 733 CBCS_N733


title: Patient 818 CBCS_N818


title: Patient 732 CBCS_N732


title: CB_72547


title: CB_72544


title: CB_72542


title: CB_72543


title: CB_72540


title: CB_72538


title: CB_72541


title: CB_72539


title: CB_72537


title: Patient 731 CBCS_N731


title: Patient 816 CBCS_N816


title: CB_72536


title: Patient 730 CBCS_N730


title: Patient 727 CBCS_N727


title: CB_72535


title: Patient 813 CBCS_N813


title: Patient 726 CBCS_N726


title: CB_72533


title: Patient 729 CBCS_N729


title: CB_72534


title: CB_72532


title: Patient 728 CBCS_N728


title: CB_72549


title: Patient 817 CBCS_N817


title: CB_72531


title: Patient 815 CBCS_N815


title: Patient 811 CBCS_N811


title: Patient 810 CBCS_N810


title: Patient 814 CBCS_N814


title: CB_72530


title: Patient 725 CBCS_N725


title: Patient 812 CBCS_N812


title: Patient 806 CBCS_N806


title: CB_72529


title: CB_72527


title: CB_72526


title: Patient 724 CBCS_N724


title: Patient 723 CBCS_N723


title: CB_72521


title: CB_72523


title: Patient 809 CBCS_N809


title: CB_72522


title: Patient 805 CBCS_N805


title: Patient 808 CBCS_N808


title: CB_72520


title: Patient 722 CBCS_N722


title: Patient 720 CBCS_N720


title: Patient 719 CBCS_N719


title: CB_72519


title: Patient 721 CBCS_N721


title: CB_72518


title: Patient 718 CBCS_N718


title: Patient 804 CBCS_N804


title: CB_72528


title: CB_72516


title: CB_72515


title: CB_72525


title: CB_72517


title: Patient 802 CBCS_N802


title: CB_72524


title: CB_72514


title: Patient 717 CBCS_N717


title: CB_72513


title: Patient 807 CBCS_N807


title: Patient 715 CBCS_N715


title: Patient 801 CBCS_N801


title: CB_72511


title: CB_72510


title: CB_72509


title: Patient 803 CBCS_N803


title: Patient 714 CBCS_N714


title: Patient 713 CBCS_N713


title: CB_72508


title: Patient 716 CBCS_N716


title: CB_72507


title: Patient 712 CBCS_N712


title: CB_72506


title: Patient 796 CBCS_N796


title: Patient 798 CBCS_N798


title: CB_72504


title: Patient 800 CBCS_N800


title: CB_72505


title: CB_72501


title: CB_72512


title: CB_72502


title: Patient 795 CBCS_N795


title: CB_72503


title: Patient 793 CBCS_N793


title: CB_72499


title: Patient 711 CBCS_N711


title: Patient 799 CBCS_N799


title: Patient 794 CBCS_N794


title: CB_72500


title: CB_72497


title: CB_72498


title: Patient 792 CBCS_N792


title: CB_72496


title: Patient 791 CBCS_N791


title: CB_72494


title: Patient 797 CBCS_N797


title: CB_72493


title: Patient 710 CBCS_N710


title: Patient 790 CBCS_N790


title: CB_72491


title: Patient 708 CBCS_N708


title: Patient 707 CBCS_N707


title: CB_72492


title: CB_72490


title: CB_72489


title: Patient 704 CBCS_N704


title: CB_72487


title: CB_72488


title: CB_72495


title: CB_72485


title: CB_72484


title: CB_72486


title: Patient 789 CBCS_N789


title: Patient 705 CBCS_N705


title: CB_72483


title: Patient 709 CBCS_N709


title: CB_64754


title: Patient 701 CBCS_N701


title: Patient 788 CBCS_N788


title: CB_64752


title: CB_64751


title: Patient 700 CBCS_N700


title: Patient 703 CBCS_N703


title: Patient 706 CBCS_N706


title: Patient 786 CBCS_N786


title: Patient 698 CBCS_N698


title: Patient 782 CBCS_N782


title: Patient 697 CBCS_N697


title: CB_64749


title: Patient 787 CBCS_N787


title: Patient 785 CBCS_N785


title: CB_64755


title: CB_64746


title: Patient 702 CBCS_N702


title: CB_64745


title: Patient 784 CBCS_N784


title: Patient 783 CBCS_N783


title: CB_64744


title: CB_64753


title: CB_64748


title: Patient 696 CBCS_N696


title: Patient 699 CBCS_N699


title: Patient 694 CBCS_N694


title: CB_64743


title: CB_64742


title: CB_64741


title: Patient 780 CBCS_N780


title: CB_64739


title: CB_64750


title: CB_64738


title: Patient 695 CBCS_N695


title: Patient 778 CBCS_N778


title: CB_64747


title: CB_64740


title: Patient 781 CBCS_N781


title: Patient 777 CBCS_N777


title: Patient 693 CBCS_N693


title: Patient 779 CBCS_N779


title: Patient 692 CBCS_N692


title: CB_64736


title: Patient 776 CBCS_N776


title: Patient 691 CBCS_N691


title: CB_64737


title: CB_64735


title: CB_64734


title: CB_64733


title: CB_64730


title: Patient 773 CBCS_N773


title: Patient 775 CBCS_N775


title: CB_64731


title: CB_64732


title: Patient 770 CBCS_N770


title: Patient 774 CBCS_N774


title: Patient 772 CBCS_N772


title: CB_64729


title: Patient 769 CBCS_N769


title: Patient 771 CBCS_N771


title: CB_64728


title: Patient 1251 CBCS_N1251


title: Patient 689 CBCS_N689


title: CB_64726


title: CB_64725


title: Patient 687 CBCS_N687


title: CB_64727


title: Patient 690 CBCS_N690


title: CB_64724


title: Patient 688 CBCS_N688


title: CB_64723


title: CB_64720


title: CB_64719


title: CB_64718


title: Patient 764 CBCS_N764


title: CB_64722


title: Patient 763 CBCS_N763


title: CB_64717


title: CB_64716


title: CB_64721


title: CB_64713


title: CB_64714


title: Patient 765 CBCS_N765


title: Patient 767 CBCS_N767


title: Patient 768 CBCS_N768


title: Patient 686 CBCS_N686


title: Patient 762 CBCS_N762


title: CB_64712


title: Patient 1184 CBCS_N1184


title: Patient 685 CBCS_N685


title: Patient 761 CBCS_N761


title: CB_64711


title: CB_64710


title: Patient 1183 CBCS_N1183


title: Patient 1180 CBCS_N1180


title: Patient 1179 CBCS_N1179


title: Patient 766 CBCS_N766


title: CB_64708


title: CB_64715


title: Patient 1181 CBCS_N1181


title: CB_64706


title: CB_64707


title: CB_64705


title: CB_64704


title: Patient 1178 CBCS_N1178


title: Patient 1182 CBCS_N1182


title: Patient 1177 CBCS_N1177


title: CB_64709


title: Patient 1176 CBCS_N1176


title: CB_64703


title: CB_64701


title: CB_64700


title: Patient 1172 CBCS_N1172


title: Patient 1175 CBCS_N1175


title: CB_64697


title: CB_64698


title: CB_64699


title: Patient 1170 CBCS_N1170


title: Patient 1173 CBCS_N1173


title: CB_64695


title: CB_64696


title: CB_64694


title: Patient 1169 CBCS_N1169


title: CB_64693


title: Patient 1171 CBCS_N1171


title: Patient 684 CBCS_N684


title: Patient 1167 CBCS_N1167


title: Patient 1174 CBCS_N1174


title: CB_64702


title: Patient 1165 CBCS_N1165


title: CB_64691


title: CB_64690


title: Patient 1164 CBCS_N1164


title: Patient 1166 CBCS_N1166


title: CB_64692


title: CB_64689


title: Patient 1163 CBCS_N1163


title: CB_64688


title: Patient 679 CBCS_N679


title: CB_64687


title: Patient 1159 CBCS_N1159


title: Patient 1162 CBCS_N1162


title: Patient 1168 CBCS_N1168


title: CB_64685


title: CB_64684


title: CB_64686


title: CB_64683


title: Patient 1158 CBCS_N1158


title: CB_64682


title: Patient 1157 CBCS_N1157


title: Patient 1161 CBCS_N1161


title: CB_64681


title: Patient 1160 CBCS_N1160


title: CB_64680


title: Patient 1154 CBCS_N1154


title: CB_64679


title: Patient 1155 CBCS_N1155


title: CB_64678


title: Patient 1150 CBCS_N1150


title: Patient 1153 CBCS_N1153


title: CB_64676


title: CB_64675


title: Patient 1151 CBCS_N1151


title: Patient 1148 CBCS_N1148


title: Patient 1152 CBCS_N1152


title: CB_64674


title: Patient 1149 CBCS_N1149


title: CB_64672


title: Patient 1147 CBCS_N1147


title: Patient 1156 CBCS_N1156


title: Patient 898 CBCS_N898


title: CB_64677


title: CB_64667


title: CB_64670


title: CB_64668


title: CB_64671


title: CB_64666


title: CB_64669


title: Patient 1146 CBCS_N1146


title: Patient 1145 CBCS_N1145


title: Patient 896 CBCS_N896


title: Patient 897 CBCS_N897


title: Patient 894 CBCS_N894


title: CB_64640


title: CB_64663


title: CB_64665


title: Patient 1144 CBCS_N1144


title: Patient 890 CBCS_N890


title: Patient 895 CBCS_N895


title: Patient 891 CBCS_N891


title: CB_64638


title: CB_64673


title: CB_64664


title: Patient 893 CBCS_N893


title: CB_64639


title: Patient 887 CBCS_N887


title: CB_64637


title: Patient 889 CBCS_N889


title: Patient 892 CBCS_N892


title: CB_64634


title: CB_64635


title: CB_64636


title: CB_64631


title: CB_64630


title: CB_64632


title: CB_64633


title: Patient 888 CBCS_N888


title: Patient 883 CBCS_N883


title: Patient 760 CBCS_N760


title: Patient 886 CBCS_N886


title: CB_64629


title: CB_64627


title: Patient 885 CBCS_N885


title: CB_64626


title: Patient 1250 CBCS_N1250


title: CB_64628


title: Patient 1249 CBCS_N1249


title: Patient 759 CBCS_N759


title: Patient 879 CBCS_N879


title: CB_64625


title: Patient 880 CBCS_N880


title: CB_64623


title: CB_64622


title: CB_64624


title: Patient 878 CBCS_N878


title: Patient 881 CBCS_N881


title: Patient 876 CBCS_N876


title: Patient 884 CBCS_N884


title: Patient 877 CBCS_N877


title: CB_64621


title: Patient 1248 CBCS_N1248


title: CB_64619


title: CB_64618


title: CB_64617


title: CB_64614


title: Patient 1246 CBCS_N1246


title: Patient 1247 CBCS_N1247


title: Patient 875 CBCS_N875


title: CB_64613


title: Patient 882 CBCS_N882


title: CB_64612


title: CB_64616


title: CB_64610


title: Patient 874 CBCS_N874


title: Patient 757 CBCS_N757


title: Patient 758 CBCS_N758


title: Patient 756 CBCS_N756


title: CB_64611


title: CB_64620


title: CB_64609


title: CB_64607


title: CB_64608


title: CB_64606


title: Patient 873 CBCS_N873


title: Patient 1245 CBCS_N1245


title: Patient 868 CBCS_N868


title: Patient 870 CBCS_N870


title: Patient 871 CBCS_N871


title: CB_64615


title: Patient 867 CBCS_N867


title: CB_64605


title: Patient 866 CBCS_N866


title: CB_64604


title: CB_64603


title: Patient 872 CBCS_N872


title: CB_64602


title: Patient 865 CBCS_N865


title: CB_64601


title: CB_64600


title: Patient 864 CBCS_N864


title: Patient 861 CBCS_N861


title: Patient 863 CBCS_N863


title: CB_64599


title: Patient 860 CBCS_N860


title: Patient 862 CBCS_N862


title: Patient 858 CBCS_N858


title: Patient 869 CBCS_N869


title: CB_64597


title: CB_64596


title: CB_64594


title: Patient 857 CBCS_N857


title: Patient 859 CBCS_N859


title: CB_64592


title: Patient 855 CBCS_N855


title: Patient 856 CBCS_N856


title: CB_64591


title: Patient 755 CBCS_N755


title: CB_64590


title: Patient 754 CBCS_N754


title: CB_64598


title: CB_64593


title: Patient 854 CBCS_N854


title: CB_64588


title: CB_64589


title: CB_64595


title: CB_64587


title: Patient 852 CBCS_N852


title: CB_64586


title: Patient 850 CBCS_N850


title: CB_64585


title: CB_64583


title: CB_64582


title: CB_64584


title: CB_64581


title: CB_64580


title: Patient 849 CBCS_N849


title: Patient 846 CBCS_N846


title: Patient 847 CBCS_N847


title: CB_64579


title: Patient 753 CBCS_N753


title: CB_64578


title: CB_64577


title: Patient 843 CBCS_N843


title: Patient 845 CBCS_N845


title: Patient 842 CBCS_N842


title: Patient 844 CBCS_N844


title: CB_64576


title: Patient 839 CBCS_N839


title: Patient 841 CBCS_N841


title: Patient 851 CBCS_N851


title: Patient 848 CBCS_N848


title: Patient 838 CBCS_N838


title: CB_64574


title: CB_64570


title: CB_64571


title: Patient 840 CBCS_N840


title: CB_64572


title: Patient 833 CBCS_N833


title: Patient 836 CBCS_N836


title: CB_64569


title: CB_64568


title: CB_64567


title: Patient 832 CBCS_N832


title: CB_64575


title: Patient 831 CBCS_N831


title: CB_64565


title: CB_64573


title: CB_64563


title: Patient 1142 CBCS_N1142


title: Patient 835 CBCS_N835


title: Patient 834 CBCS_N834


title: CB_64561


title: CB_64564


title: Patient 1143 CBCS_N1143


title: Patient 837 CBCS_N837


title: CB_64560


title: Patient 1138 CBCS_N1138


title: CB_64559


title: CB_64558


title: Patient 1137 CBCS_N1137


title: Patient 1139 CBCS_N1139


title: Patient 680 CBCS_N680


title: CB_64557


title: CB_64566


title: CB_64556


title: CB_64555


title: Patient 1133 CBCS_N1133


title: Patient 1141 CBCS_N1141


title: Patient 1136 CBCS_N1136


title: CB_64553


title: CB_64562


title: CB_64554


title: Patient 1140 CBCS_N1140


title: Patient 1130 CBCS_N1130


title: Patient 1132 CBCS_N1132


title: CB_64552


title: CB_64551


title: CB_64550


title: Patient 1135 CBCS_N1135


title: Patient 1134 CBCS_N1134


title: Patient 1126 CBCS_N1126


title: Patient 1125 CBCS_N1125


title: CB_64549


title: Patient 1123 CBCS_N1123


title: Patient 1127 CBCS_N1127


title: Patient 1131 CBCS_N1131


title: Patient 1128 CBCS_N1128


title: BC_64545


title: BC_64544


title: CB_64548


title: Patient 1129 CBCS_N1129


title: BC_64543


title: Patient 683 CBCS_N683


title: BC_64542


title: BC_64540


title: BC_64541


title: BC_64539


title: BC_64538


title: Patient 1124 CBCS_N1124


title: BC_64537


title: Patient 1122 CBCS_N1122


title: Patient 1120 CBCS_N1120


title: BC_64536


title: Patient 1117 CBCS_N1117


title: Patient 1118 CBCS_N1118


title: Patient 1119 CBCS_N1119


title: BC_64535


title: BC_64533


title: Patient 1113 CBCS_N1113


title: BC_64534


title: Patient 853 CBCS_N853


title: Patient 682 CBCS_N682


title: Patient 1111 CBCS_N1111


title: BC_64532