title: LiAg320_13

city: SEONGNAM

first: JAEHWI

last: SONG

address: SEONGNAM-DAERO 1342

department: LIFE SCIENCE

country: South Korea

institute: GACHON UNIVERSITY

data_processing:
Low quality reads were filtered according to the following criteria; reads contain more than 10% of skipped bases (marked as ‘N’s), reads contain more than 40% of bases whose quality scores are less than 20 and reads of which average quality scores of each read is less than 20. The whole filtering process was performed using the in-house scripts.
Filtered reads were mapped to the reference genome related to the species using the aligner TopHat (Trapnell C. et al, 2009).
Gene expression level was measured with Cufflinks v2.1.1 (Trapnell C. et al, 2010) using the gene annotation database of the species. To improve the accuracy of the measurement, multi-read-correction and frag-bias-correct options were applied. All other options were set to default values.
Differential expression analysis was performed by Cuffdiff (Trapnell C.et al, 2012). To enhance the analysis accuracy, multi-read-correction and frag-bias-correct options were applied. All other options were set to default values. DEGs were identified based on the qvalue threshold less than 0.05 for correcting errors caused by multiple-testing (Benjamini Y., and Hochberg Y., 1995).
GO database classifies genes according to the three categories of Biological Process(BP), Cellular Component(CC) and Molecular Function(MF) and provides information on the function of genes. To characterize the identified genes from DEG analysis, GO based trend test was carried out through the Fisher’s exact test (Fisher R. A., 1922).
Supplementary_files_format_and_content: xls, differential expressed gene (DEG) analysis
Supplementary_files_format_and_content: FPKM

source_name: human keratinocyte

growth_protocol: HaCaT keratinocyte cells, acquired from the American Type Culture Collection (ATCC, VA, USA), were cultured in high-glucose Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin in 5% CO2 under a humidified atmosphere at 37 ℃.

extract_protocol:
Total RNA was extracted from HaCaT cells using an Easy-spin Total RNA Extraction Kit (Intron Biotechnology, Korea) in accordance with the manufacturer’s instructions.
The libraries were prepared for 150bp paired-end sequencing using TruSeq RNA Sample Prep Kit (Illumina, CA, USA). Total 1μg of RNA molecules were purified and fragmented and synthesized as single-stranded cDNAs through random hexamer priming. By applying this as a template for second strand synthesis, double-stranded cDNA was prepared. After sequential process of end repair, A-tailing and adapter ligation, cDNA libraries were amplified with PCR (Polymerase Chain Reaction). Quality of these cDNA libraries was evaluated with the Agilent 2100 BioAnalyzer (Agilent, CA, USA). They were quantified with the KAPA library quantification kit (Kapa Biosystems, MA, USA) according to the manufacturer’s library quantification protocol. Following cluster amplification of denatured templates, sequencing was progressed as paired-end (2×150bp) using Illumina Novaseq6000 (Illumina, CA, USA).

treatment_protocol: HaCaT cells were seeded at 1 × 104 cells/well in 96-well plates and treated with Ag2S NPs and Li-doped Ag2S NPs at various concentrations for 24 and 48 hr.

value: keratinocyte

tag: treatment

value: treated with NPs for 24 hours

tag: concentration of nps

value: Li-doped Ag2S, 320microg/ml

tag: cell line

value: HaCaT


title: LiAg160_12

status: Public on Jul 25 2020

type: SRA

library_source: transcriptomic

molecule: total RNA

organism: Homo sapiens

tag: cell type

value: Li-doped Ag2S, 160microg/ml


title: LiAg320_15


title: CST-DsRed_2

city: Wageningen

first: Hieng-Ming

last: Ting

state: Gelderland

address: Droevendaalsesteeg 1

department: Laboratory of Plant Physiology

country: Netherlands

institute: Wageningen University

data_processing:
RNA libraries were sequenced via Illumina HiSeq 2000
The reads were assembled by using the available N. benthamiana genome sequence as template , which was carried out using the SOAPaligner/SOAP2 program with the default settings (Li et al., 2009).
The gene expression level was calculated using the RPKM method (Reads Per kb per Million reads) (Mortazavi et al., 2008).
Genome_build: N. benthamiana genome sequence (Bombarely et al., 2012)
Supplementary_files_format_and_content: Result is presented in txt table, which included the coverage of each gene, expression of gene(RPKM), total number of reads that aligned to gene, the description of gene. There are 5 columns in each xls file and meanings of each column as follows: GeneID                 GeneID        Uniq_reads_num(****)   number of reads which aligned to just one position of gene, the total uniq_reads show in the Parentheses of the front rank                               PS:Here we refer to all genes. we don't refer to the transcriptome.One gene can correspond to several transcript. The uniq_reads number result in                                 MapStatistics refer to the reads aligned to just one position of transciript        Length                 length of gene        Coverage               the coverage of gene, calculate method:the base number of all Uniq_reads in the gene/the base number of gene        RPKM                   expression, see the algorithm in help documentation of Analysis Report

organism: Nicotiana benthamiana

source_name: agroinfiltrated leaf

growth_protocol: N. benthamiana plants were grown in the greenhouse

extract_protocol:
RNA was extracted using the RNeasy® Plant Mini kit with on-column RNase-Free DNase digestion
RNA libraries were prepared for sequencing using standard Illumina protocols, performed by BGI, China

tag: tissue

value: leaf

tag: development stage

value: 5-week-old plant

tag: harvest time

value: seven days post agroinfiltration

tag: contain agrobaterium

value: CST+DsRed-RNAi


title: LiAg320_14


title: NW7

city: Pittsburgh

first: Mark

middle: Jay

last: Shlomchik

state: PA

address: W1052 Biomedical Science Tower 200 Lothrop St

department: Department of Immunology

country: USA

institute: University of Pittsburgh

data_processing:
RNA-Seq reads were aligned to hg19 with STAR with default parameters
HTSeq was used to obtain gene read counts.
DESeq2 was used to normalize the read counts for library size difference correction.
Genome_build: hg19
Supplementary_files_format_and_content: tab-delimited text file include read counts for each sample

source_name: ileum

growth_protocol: Human tissues were obtained from deceased organ donors at the time of organ acquisition for clinical transplantation as previously described, and through an approved research protocol and MTA with LiveOnNY, the organ procurement organization for the New York metropolitan area. The information on donors used in this study is presented in Supplemental Table 1. All donors were previously healthy, were free of cancer, and were serologically negative for Hepatitis B, C, and HIV. Isolation of tissues from organ donors does not qualify as "human subjects" research, as confirmed by the Columbia University IRB.

extract_protocol:
CD19+ CD27+ memory B cells (MBC) were sorted into 4 subsets defined by CD45RB and CD69 from spleen of four individual donors (D193 (D1), D256 (D2), D260 (D3), and D305 (D4), see Table S1). RNA was isolated from cell pellets using the RNeasy Mini or Micro Kit (Qiagen), and RNA concentration and quality was assessed using an Agileumnt 2100 Bioanalyzer instrument (Agileumnt Technologies).
Libraries were prepared using the Illumina TruSeq Total RNA kit with ribosomal depletion or the SMART-Seq v4 Ultra Low Input RNA Kit for sequencing and Illumina Nextera XT DNA Library preparation kit following the manufacturer's protocol

treatment_protocol: Tissue samples were maintained in cold saline and brought to the laboratory within 2-4hrs of organ procurement. Spleen and intestinal samples were processed using enzymatic and mechanical digestion resulting in high yields of live lymphocytes. Lymphocytes from blood samples were isolated by using centrifugation through a lymphopure gradient (BioLegend).

tag: b cell type

value: MBC

tag: cd69 status

tag: cd45rb  status

tag: labels

tag: donor

value: ileum


title: LiAg160_11


title: NW6


title: T-cells Donor 5

city: Zuerich

first: Yannik

last: Severin

state: ZH

address: Otto-Stern-Weg 3

department: Biology

country: Switzerland

institute: ETH Zurich

data_processing:
Basecalls performed using CASAVA version 1.8
Illumina adapters and sequences of poor quality were removed using TrimGalore v.0.6.0 with options --nextera.  polyA and polyT sequences were removed with cutadapt v.2.0 using the parameters -b A{10} -b T{10}.
Reads were aligned to the human reference genome GRCh38, v93 (Ensembl) using STAR v. 2.5.3a with default parameters and the --quantMode GeneCounts flag to obtain read counts per gene.
Reads per gene were obtained using the --quantMode GeneCounts option in STAR. Ambiguous reads (spanning multiple genes) were not counted.
Genome_build: GRCh38, v.93

source_name: Immune cells

extract_protocol:
T-cells were isolated from healthy PMBCs via a column based extraction method (Miltenyi Biotec). RNA extraction of the isolated cells was performed with a Quick-RNA MiniPrep Kit by Zymo.
cDNA libraries were obtained according to protocols published by Picelli et al, 2014. Illumina library was obtained via tagmentation using Illumina Nextera Kit. All samples were sequenced in a single run on a NovaSeq6000 (single read, 100bp, depth 20 Mio reads per sample).

value: Blood

value: T-cells

tag: disease state

value: Healthy


title: Ag320_2_9

value: Ag2S, 320microg/ml


title: cpc-1KO+cpc-1WT-2%glu-3-AT_2

city: Dallas

first: xueliang

last: lyu

state: Texas

address: 6001 Forest Park Road

department: Physiology

institute: UT Southwestern Medical Center

data_processing:
Sequencing reads were mapped to reference sequences using tophat2 with parameters --min-intron-length 4 --max-intron-length 5000 --library-type fr-firststrand --max-multihits 20 --read-mismatches 3 --read-gap-length 3 --read-edit-dist 3 --mate-inner-dist 0 --mate-std-dev 50 --no-coverage-search.
Reads Per Kilobase Million (RPKM) were calculated using cufflinks with parameters --min-intron-length 4 --max-intron-length 5000 --library-type fr-firststrand --multi-read-correct --upper-quartile-norm --frag-bias-correct
Genome_build: The reference sequence is whole genome sequence (supercontigs) with gtf annotation file from NCBI database.
Supplementary_files_format_and_content: Tab-delimited text files include RPKM values for each sample.

organism: Neurospora crassa

source_name: mycelia

growth_protocol: Fresh conidia (one week post inoculation on slants) of the WT strain and the cpc-1 deletion mutant were cultured in 2% glucose medium (1 × Vogel’s, 2% glucose) with or without 3-AT, or 0.1% glucose medium (1 × Vogel’s, 0.1% glucose and 0.17% arginine) at room temperate under constant light.

extract_protocol:
Total RNA was extracted using Trizol reagents (Invitrogen) and treated with DNase (Turbo DNase, Ambion) accoding to standard RNA extraction protocal.
Libraries were constucted using Illumina TruSeq reagents. All RNA libraries were prepared for sequencing using standard Illumina protocols.

treatment_protocol: For the samples cultured in 2% glucose medium or 0.1% glucose medium, no special treatment was performed. For the samples treated with 3-AT, the cultures were treated with 5 mM 3-AT for 8 h before sample collection.

tag: strain

value: cpc-1KO + cpc-1WT

value: mycelia

tag: culture conditions


title: cpc-1KO+cpc-1WT-2%glu-3-AT_3


title: LiAg160_10

value: keratinocyte


title: NW5

value: naive


title: Ag320_2_8

value: treated with NPs for 24 hours


title: NW4

source_name: spleen

value: spleen


title: Ag320_2_7

value: HaCaT


title: Ag160_2_6

value: Ag2S, 160microg/ml


title: NW3


title: NW2


title: Cont_2_3

value: untreated


title: cpc-1KO+cpc-1WT-2%glu-3-AT_1


title: Ag160_2_5


title: Cont_2_2


title: Cont_2_1


title: Ag160_2_4


title: Tmem106b_KO/Grn_KO_05

type: RNA

city: Munich

first: Anja

last: Capell

state: Bavaria

address: Feodor-Lynen Str 17

department: Biomedical Center (BMC)

country: Germany

institute: Ludwig-Maximilians-University

description: nCounter® Neuroinflammation panel

data_processing: NanoString data were normalized and analyzed using nSolver™ software version 4.0 (NanoString Technologies, Inc.).  The geometric mean of the 13 on-chip housekeeping genes was used for normalization of reads.

organism: Mus musculus

source_name: total brain

growth_protocol: Housing conditions included standard pellet food and water provided ad libitum, 12-hour light-dark cycle at temperature of 22 °C with maximal 5 mice per cage and cage replacement once per week and regular health monitoring.

extract_protocol: Total RNA was isolated using Qiagen RNeasy Mini Kit according to the manufacturer's protocol

tag: genotype

tag: Sex

value: female

value: brain

tag: age

value: 4.5 months


title: Tmem106b_KO/Grn_KO_04

description: nCounter® Neuroinflammation panel

value: male


title: NW1

value: MBC

value: ileum


title: P8


title: Ajuba OE 1

city: Bern

first: Daniel

last: Sanchez-Taltavull

address: Murtenstrasse 35

department: Department of BioMedical Research

institute: University of Bern

data_processing:
Samples were aligned to the human reference genome GRCh38 with HISAT2
read counts were obtained from the BAM files with featureCounts
Genome_build: GRCh38
Supplementary_files_format_and_content: read counts matrix

growth_protocol: The cells were cultured in Dulbecco's Modified Eagle's Medium GlutaMAX (with 10% FBS, 100 μg/mL Penicillin/streptomycin (Life Technology)) at 37°C in a humidified incubator with 5% CO2. All lines have been tested and are negative for mycoplasma contamination using PCR Mycoplasma Test Kit (Promokine).

extract_protocol: We isolated RNA from SW480 cells using the ReliaPrep RNA cell 2100 BioAnalyzer. The quality and concentration were measured with the Agilent 2100 BioAnalyzer. The isolated RNA was then sent for sequencing according to the following parameters: paired-end with reads of 50 bp, TruSeq Stranded mRNA. The RNA was sequenced with a NovaSeq6000.

treatment_protocol: CRC cell line SW480 were transduced with two independent shRNAs targeting Ajuba and one lentiviral Ajuba Overexpressing construct. All experiments were carried out on cells at 25-50% confluence. Cells were transfected with lentiviral supernatant, containing shRNA targeting Ajuba or Ajuba OE construct in DMEM with 10% FBS. The viral supernatant was added onto the cultured cells in a total volume of 900 μl and incubated for 3h then centrifuged at 1400 rpm for 5 min, incubated for an additional 3h, then complete medium was added to a final volume of 2ml and incubated for 48h. Two different shAjuba viruses were purchased from MISSIONTM, Sigma-Aldrich.   • Clones shAjuba1: in the coding region NM-032876.4-1385s1c1   • Clone shAjuba2: in the non-coding region 2URT NM_032876.4-2786s1c1   In order to control for the lentiviral transduction, we transduced the colon cell lines with a scrambled control shRNA with random sequence   • Clone shScrambled MISSION® pLKO.1-puro Non-Mammalian shRNA Control Plasmid   In order to overexpress Ajuba, CRC cell line was transduced with lentivirus containing an Ajuba overexpressing construct   • Clone Ajuba OE Harvard PlasmID:Phage_CMV_C_FLAG_HA_IRES_PURO  As a control for the AJUBA overexpressed cells, CRC cell lines were transduced with the Ajuba overexpressing construct, where we previously clonally excised the exogenous Ajuba sequence. Stable cell lines were then negatively selected using 1.5 μg/mL puromycin (Life Technologies). The efficiency of the transduction was assessed by real-time qRCR and immunoblot.

tag: cell line

value: Colon adenocarcinoma cell line

tag: passages

value: 10-18 passages

tag: treatment

value: lentiviral construct  Ajuba  OE sequence


title: Ajuba OE 2

tag: cell type


title: Tmem106b_KO/Grn_KO_03

description: nCounter® Neuroinflammation panel

tag: tissue


title: Ajuba OE 3


title: Control OE 3

value: lentiviral construct without exogenous Ajuba sequence


title: P7


title: Tmem106b_KO/Grn_KO_01

description: nCounter® Neuroinflammation panel


title: Tmem106b_KO/Grn_KO_02

description: nCounter® Neuroinflammation panel


title: Control OE 2


title: P6


title: shAjuba 2.3

value: lentiviral shRNA targeting non-coding region of Ajuba


title: Control OE 1


title: Grn_KO_04

description: nCounter® Neuroinflammation panel


title: shAjuba 2.2


title: P5


title: shAjuba 2.1


title: shAjuba 1.3

value: lentiviral shRNA targeting coding region of Ajuba


title: Grn_KO_03

description: nCounter® Neuroinflammation panel


title: shAjuba1.2


title: shAjuba1.1


title: P3

value: naive


title: Grn_KO_02

description: nCounter® Neuroinflammation panel


title: shScrambled2

value: lentirival shRNA with random sequence


title: P4

value: spleen


title: Parental 2

value: unmodified cell line


title: Grn_KO_01

description: nCounter® Neuroinflammation panel


title: shScrambled1


title: Tmem106b_KO_04

description: nCounter® Neuroinflammation panel


title: Parental 1


title: P1


title: Tmem106b_KO/Grn_KO_17

description: nCounter® Neuropathology panel

data_processing: NanoString data were normalized and analyzed using nSolver™ software version 4.0 (NanoString Technologies, Inc.).  The geometric mean of the 10 on-chip housekeeping genes was used for normalization of reads.

tag: gender


title: Tmem106b_KO/Grn_KO_18

description: nCounter® Neuropathology panel


title: Tmem106b_KO/Grn_KO_16

description: nCounter® Neuropathology panel


title: Grn_KO_15

description: nCounter® Neuropathology panel


title: Grn_KO_14

description: nCounter® Neuropathology panel


title: Tmem106b_KO_01

description: nCounter® Neuroinflammation panel


title: WT_08

description: nCounter® Neuroinflammation panel

value: WT


title: Grn_KO_13

description: nCounter® Neuropathology panel

value: female


title: P2

tag: b cell type


title: Tmem106b_KO_11

description: nCounter® Neuropathology panel

value: brain


title: WT_07

description: nCounter® Neuroinflammation panel

value: 4.5 months


title: Tmem106b_KO_10

description: nCounter® Neuropathology panel

value: male


title: Tmem106b_KO_03

description: nCounter® Neuroinflammation panel


title: WT_06

description: nCounter® Neuroinflammation panel


title: WT_02

description: nCounter® Neuropathology panel


title: WT_01

description: nCounter® Neuropathology panel


title: Tmem106b_KO_02

description: nCounter® Neuroinflammation panel


title: WT_05

description: nCounter® Neuroinflammation panel


title: C2C12_D2_B1KD-3

city: Sendai

first: Mitsuyo

last: Matsumoto

address: 2-1 Seiryo-machi Aoba-ku

department: Biochemistry

country: Japan

institute: Tohoku university school of medicine

description:
US80703200_257480912556_S01_GE1_107_Sep09_2_4.txt
Muscle
Gene expression of day 2 after induced differentiation in siBACH1-transfected C2C12 cells.

hyb_protocol: Cy3-labelled cRNA was fragmented at 60°C for 30 minutes in a reaction volume of 25 ul containing 1x Agilent fragmentation buffer and 2x Agilent blocking agent following the manufacturer's instructions. On completion of the fragmentation reaction, 25 ul of 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to Agilent Whole Mouse Genome Oligo Microarrays (Agilent-074809) for 17 hours at 65°C in a rotating Agilent hybridization oven. After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent) and 1 minute with 37°C GE Wash buffer 2 (Agilent), then dried immediately by brief centrifugation.

scan_protocol: Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner using one color scan setting for 8x60k array slides.

data_processing: The scanned images were analyzed with Feature Extraction Software 9.1 (Agilent) using default parameters (protocol GE1_107_Sep9 and Grid: 028005_D_F_20101029) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.

source_name: C2C12, Mouse C3H muscle myoblast, day2, siBach1, rep3

label_protocol: Total RNA was labeled with indocarbocyanine through the use of a CyDye post-labeling Reactive Dye Pack (Amersham).

growth_protocol: Cells were cultured at 37℃ in 5% CO2 in high-glucose Dulbecco’s modified Eagle medium (DMEM, Sigma) containing 10% fetal bovine serum and 1% penicillin/streptomycin (10,000 units of penicillin per ml, 10,000 μg of streptomycin per ml) (GIBCO). C2C12 cells were passaged before confluence. C2C12 cells were induced to differentiate into myotubes by replacing in the DMEM with 2% horse serum (HS) when the cells reached confluence.

extract_protocol: RNA was isolated with an RNeasy mini kit (Qiagen) according to the manufacturer's protocol.

treatment_protocol: C2C12 cells were treated with DMEM with 2% horse serum (HS)  when the cells reached the confluence to induction of differentiation, that is, 24 hours post-transfection with siRNA.

value: Myoblast cell line

tag: sirna

tag: time point

value: Day2 differentiation


title: Bulk_LG_Normal_96hAEL

city: Seoul

first: Jin-Wu

last: Nam

address: Seongdong-Gu Hangdang-dong

department: Department of Life Science

institute: Hanyang University

data_processing:
Low-quality reads were filtered using Sickle (version 1.33) when Phred base quality ≤ 20 or read length ≤ 20, and quality was examined using FastQC (v0.11.8).
The remaining reads were mapped to Drosophila reference genome (BDGP 6.02, September 2014) using STAR (version 2.5.3a) with default parameters.
Alignment quantification was performed using featureCounts (version 1.6.0) with <-t exon> and <-g gene_id> parameters.
Genome_build: BDGP 6.02 (September 2014)
Supplementary_files_format_and_content: Tab-delimited text files include read counts for each sample.

organism: Drosophila melanogaster

growth_protocol: To synchronize larval stages, one hundred adult flies were kept on grape-juice agar plate for two hours. Hatched larvae were discarded at 23 hours after egg laying (AEL), and those at 24 hours AEL were collected and reared on normal corn-meal yeast media.

extract_protocol:
Intact LGs were collected from larvae at corredponing time points. Instead of Papain solution, Trizol (MRC, TR118) is added to LGs and performed RNA extraction. More than 1ug of total RNA were prepared for each experiment.
cDNA library was constructed by 5’ and 3’ adapter ligation and loaded into a flow cell where fragments are captured into library adapters. Each fragment is then amplified through bridge amplification. After cluster generation finished, templates are sequenced by Illumina Trueseq.

value: Oregon-R

tag: condition

value: steady state

tag: hours after egg laying (h ael)

value: 96 h AEL

value: Lymph gland


title: Bulk_LG_Normal_120hAEL

value: 120 h AEL


title: WT_04

description: nCounter® Neuroinflammation panel


title: Tmem106b_KO_09

description: nCounter® Neuropathology panel


title: WT_03

description: nCounter® Neuropathology panel


title: C2C12_D2_C-3

description:
US80703200_257480912556_S01_GE1_107_Sep09_2_2.txt
Muscle
Gene expression of day 2 after induced differentiation in siControl-transfected C2C12 cells.

source_name: C2C12, Mouse C3H muscle myoblast, day2, siControl, rep3

value: siControl


title: 13_kontrola_TF

city: Kraków

first: Agnieszka

middle: Małgorzata

last: Domka

address: Gronostajowa 7a

department: Małopolska Centre of Biotechnology

country: Poland

institute: Jagiellonian University

data_processing:
trimming low quality bases and the adaptor sequence was removed to obtain high-quality clean reads using cutadapt version 1.8.1.
The pre-processed high-quality reads were mapped to A. thaliana genome with HISAT2 version 2.1.0 using the default parameters.
The resulted output of HISAT2 was employed to determine the differentially expressed genes using Cuffdiff tool in Cufflinks version 2.2.1 pipeline with default parameters.
We considered up- and down-regulated, those genes having p-value < 0.05 and they were used for downstream functional analysis. The DEGs selected were annotated using A. thaliana genome.
Genome_build: Arabidopsis thaliana TAIR10.1

organism: Noccaea goesingensis

extract_protocol:
Total RNA Mini Kit (Bio-Rad, USA) was used for total RNA extraction from roots (from 5 plants per sample). RNA digestion was performed with DNase (DNA free kit, Ambion Bioscience, USA) and RNA purity and quantity were analyzed by Biospec-Nano (SHIMADZU, JP). The integrity of RNA was assessed with the Agilent 2100 Bioanalyzer (USA) and RNA 6000 Nano Kit (Agilent, DE).
Total RNA-seq Kit v2 (ThermoFisher Scientific, USA) was utilized for whole transcriptome libraries preparation. Poly(A) RNA selection was performed with 1000 ng of total RNA with the use of Dynabeads mRNA DIRECT Micro Kit (ThermoFisher Scientific, USA) following manufacturer’s protocol. Next, the poly(A) RNA was fragmented with RNase III, purified and checked for the quality with Agilent 2100 Bioanalyzer (Agilent Technologies, USA) and the RNA 6000 Pico Kit (Agilent Technologies, USA).

treatment_protocol: Seedlings were inoculated in vitro, in MSR medium and after 10 days transplanted to pot cultures to sterile substrate from mine dump "Bolesław" mixed with sterile sand in a 2:1 v:v ratio. Multipots were placed in 23x45x18 cm plexiglass chambers covered with polyamide gauze. Plants were grown for 28 days in a Panasonic MLR-352H-PE, JPN chamber with a 16h photoperiod, 100-120 μmol·m-2·s-1 PAR,  21/17°C day/night temperature and 50% humidity.

tag: ecotype

value: non-metallicolous

value: untreated (control)

value: roots


title: Bulk_LG_Normal_72hAEL

tag: strain

value: 72 h AEL


title: C2C12_D0_B1KD-3

description:
US80703200_257480912556_S01_GE1_107_Sep09_2_3.txt
Muscle
Gene expression without induced differentiation in siBACH1-transfected C2C12 cells.

source_name: C2C12, Mouse C3H muscle myoblast, day0, siBach1, rep3

value: Day0 differentiation


title: C2C12_D0_C-3

description:
US80703200_257480912556_S01_GE1_107_Sep09_2_1.txt
Muscle
Gene expression without induced differentiation in siControl-transfected C2C12 cells.

source_name: C2C12, Mouse C3H muscle myoblast, day0, siControl, rep3


title: 12_kontrola_TF


title: RNA-seq high light and low temperature stress rep2

city: Daejeon

first: Byung-Kwan

last: Cho

address: 291 Daehak-ro

institute: KAIST

data_processing:
Basecalls performed using CASAVA version 1.4
All sequencing reads were mapped to E. coli MG1655 reference genome (NC_000911.1, NC_005229.1, NC_005230.1, NC_005231.1, NC_005232.1) using CLC Genomics Workbench5 with the length fraction of 0.7, the similarity of 0.9, mismatch score -2, insertion score -3, and deletion score -3.
To identify differentially expressed genomic region independently to current annotation, we detected transfrag that are contiguously transcribed regions above specified cutoff. In order to reduce possible over fragmentation, we merged nearby transfrags into a transfrag if inter-distance is less than 20 nt and then discarded transfrags that are shorter than 20 nt.
The expression levels were calculated by DESeq (http://genomebiology.com/2010/11/10/R106) based upon the reads count with strand-specific manner.
Supplementary_files_format_and_content: gff file is generated by in-house script (refer article for detail method)

organism: Synechocystis sp. PCC 6803

growth_protocol: Glucose-tolerant Synechocystis sp. PCC6803 cells were grown photoautotrophically at 30°C under illumination from fluorescent lamps at 20 PPF in BG-11 medium, with aeration by air that contained 1% CO2. They were cultivated in 1 L flask containing 400 mL BG11 medium in a shaking incubator.

extract_protocol:
Harvested Synechocystis cells were centrifuged and resuspended with lysis buffer, 20 mM Tris-HCl (pH 7.5), 140 mM NaCl, 5 mM MgCl2, and 1% Triton-X of 300 ul. Further, the resuspended cells were dropped into a mortar packed with liquid nitrogen, and grinded with a pestle. Then the lysate was centrifuged and for efficient RNA extraction from cells, the supernatant was further chemically lysed with QIAzol lysis reagent (Qiagen, Hilden, Germany) of 700 µl for 5 min at room temperature according to miRNeasy mini kit manual (Qiagen). The lysate was incubated with chloroform of 140 µl for 2-3 min at 4 ℃ and centrifuged for 15 min at 3000 rpm. The supernatant was mixed with 1.5 fold 100% ethanol and the RNA transcript was purified through RNeasy mini spin column (Qiagen). To confirm the quality of extracted RNAs, total RNA was visualized using agarose gel electrophoresis. rRNA from total RNA was removed using the RiboZeroTM magnetic kit for bacteria (Epicenter, Madison, WI) according to the manufacturer’s manual. The removal of rRNA was verified using Experion automated electrophoresis system (Biorad, Hercules, CA).
The library preparation for transciptome analysis was achieved using ScriptSeq™ v2 RNA-Seq Library Preparation Kit (Epicentre, Madison, WI) according to the manumacturer’s manual. In brief, rRNA-depleted RNA of 50 ng was fragmented and reverse transcribed to cDNA using six random-sequence primers containing a tagging sequence at their 5′ ends. Then cDNA was tagged at 3′ end using the Terminal-Tagging Oligo (TTO). The cDNA tagged at both its 5’- and 3’-end is purified by Agencourt AMPure XP system (BeckmanCoulter, Brea, CA) and amplified by 10-cycle PCR using PCR primers that anneal to the tagging sequences and contain the adaptor sequences required for cluster generation and index code. Excess nucleotides and PCR primers are removed from the library using the AMPure XP System. The quality of DNA library and its concentration was calculated using ExperionTM system and Qubit®2.0 fluorometer (Invitrogen, Carlsbad, CA). Finally, validated DNAs were sequenced using Mi-Seq (Illumina, San Diego, CA) and Miseq® V2 reagent kit of 50 cycles according to manufacturer’s manual.

treatment_protocol: At the exponential phase of growth (when the optical density at 730 nm of the culture was 0.8), cells were treated with various stress conditions. Cells were exposed at low temperature, at high light intensity or both. Low temperature conditions were 24C for 30 min, and high light intensity conditions were 300 PPF for 30 min. All experiments were performed as triplicates.


title: C2C12_D0_B1KD-2

description:
US80703200_257480912555_S01_GE1_107_Sep09_2_3.txt
Muscle
Gene expression without induced differentiation in siBACH1-transfected C2C12 cells.

source_name: C2C12, Mouse C3H muscle myoblast, day0, siBach1, rep2


title: RNA-seq high light and low temperature stress rep1


title: Col-D rep1

city: Shanghai

first: weiwei

last: Qi

address: No 99 Shangda Road

country: China

institute: Shanghai  University

data_processing:
Illumina Casava1.8 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to TAIR 10 whole genome using bowtie0.12.5  with parameters default
Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using a protocol from Mortazavi et al,Nature methods 2008.Replicate sample's reads were merged to count.
Genome_build: TAIR 10
Supplementary_files_format_and_content: tab-delimited text files include RPKM values for each Sample

organism: Arabidopsis thaliana

source_name: seedling

growth_protocol: Seeds were germinated on 1/2 MS plates plus 1% sucrose and placed at 4°C for 4 days and then tranferred to white light for 12 h before placed in darkness or red light for another 4 days

extract_protocol:
RNA was extracted with RNAprep plant kit from TIANGEN, followed by DNase I (Takara) treatment. Quality control was performed with Agilent Bioanalyser. mRNA was purifed with Dynabeads® Oligo (dT)25（Life).
RNA libraries were prepared for sequencing using standard Illumina protocols

value: WT

tag: light condition

value: Dark

tag: growth time

value: 4.5 days


title: 8_kontrola_TR

value: metallicolous


title: C2C12_D2_B1KD-2

description:
US80703200_257480912555_S01_GE1_107_Sep09_2_4.txt
Muscle
Gene expression of day 2 after induced differentiation in siBACH1-transfected C2C12 cells.

source_name: C2C12, Mouse C3H muscle myoblast, day2, siBach1, rep2

value: Myoblast cell line


title: C2C12_D0_C-2

description:
US80703200_257480912555_S01_GE1_107_Sep09_2_1.txt
Muscle
Gene expression without induced differentiation in siControl-transfected C2C12 cells.

source_name: C2C12, Mouse C3H muscle myoblast, day0, siControl, rep2


title: 7_kontrola_TR


title: RNA-seq low temperature stress rep1

source_name: low temperature stress rep1


title: RNA-seq high light stress rep2

source_name: high light stress rep2


title: Sa_J1074/R2_talc_production_phase_B

city: Bielefeld

first: Christian

last: Ruckert-Reed

address: Sequenz 1

department: Medical School OWL

institute: Bielefeld University

data_processing:
Basecalls were performed using RTA v 1.18.66.3
Sequenced reads were mapped to the CP059254.1 whole genome using bowtie2 v2.3.2 with parameters -X 600
Raw read counts per CDS were calculated using ReadXplorer v.2.2.3 with addition of one pseudocount per CDS
Background correction was performed by substraction of the median read count derived from the non-transcribed strand over all CDS, adjusetd for feature length
Read cound normalization and data analysis were performed using DESeq2
Genome_build: CP059254.1
Supplementary_files_format_and_content: tab-delimited text files contain normalized read counts derived via DESeq2 for each CDS
Supplementary_files_format_and_content: Matrix table with background corrected raw gene counts for every CDS and every sample

organism: Streptomyces albidoflavus

source_name: cell culture grown in SGG with added talc microparticles

growth_protocol: Liquid SGG medium was used for pre- and main cultures for pamamycin production and contained per liter: 10 g soluble starch (Sigma-Aldrich), 10 g glycerol, 2.5 g corn steep powder (Sigma-Aldrich), 5 g bacto peptone (Becton & Dickinson), 2 g yeast extract (Becton & Dickinson), 1 g sodium chloride, and 21 g MOPS. The pH of the medium was adjusted to 7.2, using 6 M NaOH. One loop of spores was scratched from a five-day old plate culture and used to inoculate a liquid pre-culture, which was grown overnight in a 500 mL baffled shake flask with 50 mL medium and 30 g soda-lime glass beads (5 mm, Sigma-Aldrich). When the pre-culture reached the late exponential phase, an appropriate amount of cells was collected (8500 x g, room temperature, 5 min), resuspended in 10 mL fresh medium, and used to inoculate the main-culture (50 mL medium in 500 mL baffled shake flasks). All cultivation experiments were conducted in triplicate on a rotary shaker (28 °C, 230 rpm, 75% relative humidity, 5 cm shaking diameter, Multitron, Infors AG, Bottmingen, Switzerland).

extract_protocol:
RNA was extracted with the Qiagen RNA Mini kit (Qiagen, Germany) according to the manufacturer’s instructions. Residual DNA was removed by digestion with 10 U RNase-free DNase I (Thermo Scientific) for 1 h in the presence of RiboLock RNase inhibitor (Thermo Scientific). After DNA digestion, the RNA was again purified with the same kit. RNA quality was checked by Trinean Xpose (Gentbrugge, Belgium) and the Agilent RNA 6000 Nano Kit on an Agilent 2100 Bioanalyzer (Agilent Technologies, Böblingen, Germany).
Ribosomal RNA molecules were removed from total RNA with the Ribo-Zero rRNA Removal Kit (Illumina, San Diego, USA) and removal of rRNA was checked with the Agilent RNA 6000 Pico Kit on an Agilent 2100 Bioanalyzer. Libraries of cDNA were prepared with the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, USA), and the resulting cDNA was sequenced paired end on an Illumina MiSeq system using 75 bp read length.

treatment_protocol: The control culture was grown in pure SGG medium. For the experiment culture, talc microparticles (hydrous magnesium silicate, 3MgO∙4SiO2∙H2O, 10 µm, Sigma-Aldrich) were resuspended in 50 mM Na-acetate buffer (pH 6.5), autoclaved at 121°C for 20 min, and added to the sterile medium prior to inoculation. Samples were taken during growth phase (5h after inoculation) and main production phase (21h after inoculation).

value: talc:grown in liquied medium with added talc microparticles

tag: time


title: RNA-seq high light stress rep1

source_name: high light stress rep1


title: Sa_J1074/R2_talc_production_phase_A

value: production phase (21h after inoculation)


title: Sa_J1074/R2_talc_production_phase_C


title: Sa_J1074/R2_talc_growth_phase_C

value: growth phase (5h after inoculation)


title: Sa_J1074/R2_talc_growth_phase_B


title: Sa_J1074/R2_talc_growth_phase_A


title: 10_kontrola_TR


title: RNA-seq control rep2

source_name: control rep2


title: 11_kontrola_TF


title: Sa_J1074/R2_control_production_phase_C

source_name: cell culture grown in pure SGG


title: C2C12_D0_B1KD-1

description:
US80703200_257480912555_S01_GE1_107_Sep09_1_3.txt
Muscle
Gene expression without induced differentiation in siBACH1-transfected C2C12 cells.

source_name: C2C12, Mouse C3H muscle myoblast, day0, siBach1, rep1


title: 11_kontrola_Ni_TR

value: Ni treated


title: C2C12_D2-C-1

description:
US80703200_257480912555_S01_GE1_107_Sep09_1_2.txt
Muscle
Gene expression of day 2 after induced differentiation in siControl-transfected C2C12 cells.

source_name: C2C12, Mouse C3H muscle myoblast, day2, siControl, rep1

value: Day2 differentiation


title: RNA-seq control rep1

source_name: control rep1


title: C2C12_D2_C-2

description:
US80703200_257480912555_S01_GE1_107_Sep09_2_2.txt
Muscle
Gene expression of day 2 after induced differentiation in siControl-transfected C2C12 cells.

source_name: C2C12, Mouse C3H muscle myoblast, day2, siControl, rep2


title: C2C12_D2_B1KD-1

description:
US80703200_257480912555_S01_GE1_107_Sep09_1_4.txt
Muscle
Gene expression of day 2 after induced differentiation in siBACH1-transfected C2C12 cells.

source_name: C2C12, Mouse C3H muscle myoblast, day2, siBach1, rep1


title: C2C12_D0_C-1

description:
US80703200_257480912555_S01_GE1_107_Sep09_1_1.txt
Muscle
Gene expression without induced differentiation in siControl-transfected C2C12 cells.

source_name: C2C12, Mouse C3H muscle myoblast, day0, siControl, rep1


title: Sa_J1074/R2_control_production_phase_A


title: RNA-seq low temperature stress rep2

source_name: low temperature stress rep2


title: HePG2_S3

city: Hangzhou

first: Xiujun

last: Cai

state: Zhejiang

address: 3 East Qingchun Road

department: Department of General Surgery

institute: Sir Run-Run Shaw Hospital, Zhejiang University School of Medicine

description: circRNA expression under Sorafenib treatment

hyb_protocol: The labeled cRNAs were hybridized onto the Arraystar Human circRNA Array (8x15K, Arraystar).

scan_protocol: After having washed the slides, the arrays were scanned by the Agilent Scanner G2505C.

data_processing: Agilent Feature Extraction software (version 11.0.1.1) was used to analyze acquired array images. Quantile normalization and subsequent data processing were performed using the R software package. Differentially expressed circRNAs with statistical significance between two groups were identified through Volcano Plot filtering. Differentially expressed circRNAs between two samples were identified through Fold Change filtering. Hierarchical Clustering was performed to show the distinguishable circRNAs expression pattern among samples.

source_name: Cultured cells under 48hrs of 7μM Sorafenib treatment

label_protocol: The enriched circular RNAs were amplified and transcribed into fluorescent cRNA utilizing a random priming method (Arraystar Super RNA Labeling Kit; Arraystar).

growth_protocol: All the cell lines were cultured in Dulbecco's Modified Eagle's Media (Invitrogen, Grand Island, NY, USA) supplemented with 10% FBS (v/v), penicillin (25 units/ml), streptomycin (25 g/ml), 1% L-glutamine, and 10% fetal bovine serum (FBS), in a 5% (v/v) CO2 humidified incubator at 37℃.

extract_protocol: For RNA extraction, total RNAs were isolated using Trizol reagent (Invitrogen, Grand Island, NY). Total RNA from each sample was quantified using the NanoDrop ND-1000. The sample preparation and microarray hybridization were performed based on the Arraystar’s standard protocols. Briefly, total RNAs were digested with Rnase R (Epicentre, Inc.) to remove linear RNAs and enrich circular RNAs.

treatment_protocol: Under 48hrs of 7μM Sorafenib treatment

tag: sorafenib response

value: resistant


title: HePG2_S2

tag: cell line


title: 6_kontrola_TR


title: M1_SARS-CoV-2 infection_2hr

city: St. Louis

first: Siyuan

last: Ding

state: MO

address: 425 S. Euclid Ave., MPRB, Rm 9220

department: Molecular Microbiology

institute: Washington University in St. Louis

description: Sample 4

data_processing:
DNBseq
SE 50bp
20 M Clean reads per sample
The SE reads were aligned to the hg19 build using Bowtie2 to map clean reads to reference gene and using HISAT2 to reference genome with the following parameters: --phred64 --sensitive -I 1 -X 1000. Reads were counted using Subread and differential gene expression analysis was performed using DESeq2BGI data was analyzed using DESeq2.
Genome_build: Homo_sapiens GCF_0000001405.38_GRCh38.p12
Supplementary_files_format_and_content: Complete gene expression.txt (RPKMs, read counts)

source_name: M1_SARS-CoV-2 infection_2h

extract_protocol:
Trizol extraction and RNA quality control by Agilent 2100 Bioanalyzer
DNBseq RNA-Seq Quantification Library

value: macrophages

tag: infection


title: Sa_J1074/R2_control_growth_phase_C

tag: treatment


title: 14_kontrola_Ni_TF

value: non-metallicolous


title: HePG2_S1


title: M1_mock

description: Sample 3

tag: cell type

value: control


title: DC_SARS-CoV-2 infection_2hr

description: Sample 2

source_name: DC_SARS-CoV-2 infection_2h

value: dendritic cells


title: 2_kontrola_Ni_TF

tag: tissue

value: roots


title: DC_mock

description: Sample 1


title: Sa_J1074/R2_control_growth_phase_A


title: 1_kontrola_Ni_TF


title: Sa_J1074/R2_control_growth_phase_B


title: 1o_kontrola_Ni_TR


title: HePG2_2

value: sensitive


title: HePG2_1


title: HePG2_3


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S025

status: Public on Jul 24 2020

city: Jerusalem

first: Matan

last: Hofree

address: The Edmond J. Safra, Givat Ram Campus

department: School of Computer Science and Engineering | The Lautenberg Center for Immunology and Cancer Research

country: Israel

institute: The Hebrew University of Jerusalem

data_processing:
bcl2fastq was used to extract invidual cell read files
Transcript/gene counts estimated using RSEM, using STAR for alignment
Picard-Tools CollectRNASeqMetrics is applied to each bam
QC filtering
Sequencing depth normalization
log2(transcripts/100K + 1)
Genome_build: Gencode GRCm38.p5 (M15)
Supplementary_files_format_and_content: Raw count matrices are in HDF5 Feature Barcode Matrix Format, and metadata files are comma-delimited text:
mmLungPlate_fQC_dSp_normTPM.h5 (hdf5, (i,j,v, sparse matrix), Log2 (transcript/100K+1))
mmLungPlate_fQC_dSp_rawCount.h5 (hdf5, (i,j,v, sparse matrix), Raw counts, after depth normalization.)
mmLungPlate_fQC_dSp_rawCountOrig.h5 (hdf5, (i,j,v, sparse matrix), Raw counts )
mmLungPlate_fQC_dZ_QCstat_smpTable.csv (csv, QC table)
mmLungPlate_fQC_dZ_annot_smpTable.csv (csv, sample annotations)
mmLungPlate_fQC_geneTable.csv (csv, gene ids)
mmLungPlate_fQC_smpTable.csv (csv, sample ids)

source_name: Lung tissue, AT2 cells

growth_protocol: We used C57BL/6 x Sv129 mixed background mice from the following previously published strains: KrasLSL-G12D (Jackson et al., 2001), Trp53flox/flox (Marino et al., 2000), Rosa26LSL-tdTomato (Madisen et al., 2010), and Rosa26LSL-Luciferase (Safran et al., 2003).

extract_protocol:
Mice with LUAD tumors were euthanized at 2, 12, 20, or 30 weeks following tumor induction. We chose these time points because they reflect key stages in LUAD progression: atypical adenomatous hyperplasia (AAH) (KT and KPT at 2 weeks), adenoma (KT at 12 and 30 weeks), adenoma-to-LUAD transition (KPT at 12 weeks) and LUAD (KPT at 20 and 30 weeks). We micro-dissected large KPT tumors individually at 20 and 30 weeks, whereas all other samples were harvested by dissociating entire lungs containing mixtures of neoplasias in various stages of tumor progression. Following euthanasia, mice were perfused with S-MEM (Gibco, catalog #11380037) through the right ventricle of the heart. Dissected lungs or microdissected tumors were dissociated either with protease and DNAse solution in the Lung Dissociation Kit (Miltenyi Biotech, catalog #130-095-927) followed by mechanical dissociation using gentleMACS “C” columns (Miltenyi Biotech, catalog #130-093-237) according to the manufacturer’s instructions (Tammela et al., 2017), or by a mixture of Dispase II (Gibco, catalog #17105-041, final concentration 0.6 U/ml), Collagenase Type IV (Thermo Fisher Scientific, catalog #17104019; final concentration 0.083 U/ml), and DNase I (Sigma-Aldrich, catalog #69182-3; final concentration 10 U/ml) in S-MEM solution containing Gentamicin (Gibco, catalog #15750-060, final concentration 20 mg/ml) at 37°C for 30 min (Table S6). The dissociated cells were filtered using a 100 mm strainer and spun at 300 g for 5 min at room temperature. The supernatant was removed by aspiration and red blood cell lysis was performed using ACK (Thermo Fisher Scientific, catalog #A1049201). Cells were then washed with media and pelleted at 300 g for 5 min at 4°C. The supernatant was removed, and the pellet resuspended in Fluorescence-Activated Cell Sorting (FACS) buffer media (200 mM EDTA with 250 ml heat-inactivated FBS in PBS) before being passed through a 40 mm strainer and counted for use in FACS below.
Cells were dissociated as above, stained with DAPI and live cells were sorted as described above into 96 well plates containing 5 ml of TCL Buffer (Qiagen, catalog #1031576) with 1% beta-mercaptoethanol. Plates were processed by a modified SMART-Seq2 protocol (Picelli et al., 2013), with the following modifications: RNA from single cells was first purified with Agencourt RNAClean XP beads (Beckman Coulter, catalog #A63881) using Bravo Automated Liquid Handling Platform prior to oligo-dT primed reverse transcription with Maxima reverse transcriptase (Thermo Fischer, catalog #EP0752) and locked TSO oligonucleotide (Exiqon, custom made), which was followed by a 21 cycle PCR amplification using KAPA HiFi HotStart ReadyMix (KAPA Biosystems, catalog #KK2601). The WTA product was purified using Agencourt AMPure XP beads (Beckman-Coulter, catalog #A63881) and a Bravo Automated Liquid Handling Platform. Libraries were tagmented using the Nextera XT Library Prep kit (Illumina, catalog #FC-131-1096) with custom barcode adapters. Libraries from 384 cells with unique barcodes were combined and sequenced using a NextSeq 500 sequencer (Illumina, catalog #FC-404-2005) at the Broad Genomics Platform.
SmartSeq2

treatment_protocol: Tumors were induced in K or KP mice with 0.5-2.5 x 10^8 PFU of AdSPC-Cre (Sutherland et al., 2011) or 2 x 10^8 PFU of AdCMV-FlpO (Iowa).

tag: timepoint

tag: breed

tag: tumor

tag: plate

tag: sack_timepoint (weeks)

tag: age (weeks)

tag: Sex

value: female


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S021

tag: strain


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S028


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S035


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S022


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S027


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S019


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S017


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S031


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S032


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S026


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S033


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S018


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S038


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S023


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S036


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S034


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S037


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S030


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S015


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S016


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S024


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S014


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S029


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S012


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S020


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S010


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S011


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S013


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S009


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S094


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S090


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S096


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S089


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S003


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S088


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S091


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S005


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S093


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S085


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S092


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S002


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S001


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S086


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S082


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S008


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S006


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S007


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S080


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S079


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S083


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S081


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S078


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S077


title: Single-cell RNAseq of lung tumor: T_0w_ND_f2_T0_P1_S004


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S076


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S095


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S087


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S084


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S075


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S072


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S073


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S069


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S068


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S066


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S065


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S067


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S058


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S063


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S064


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S057


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S056


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S062


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S053


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S055


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S061


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S054


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S051


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S050


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S048


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S049


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S071


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S045


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S047


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S070


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S060


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S046


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S059


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S044


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S043


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S040


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S041


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S042


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S052


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S038


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S039


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S036


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S037


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S033


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S030


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S029


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S035


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S028


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S031


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S027


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S025


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S024


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S026


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S022


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S020


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S021


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S023


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S019


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S018


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S017


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S016


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S034


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S015


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S032


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S013


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S014


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S010


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S009


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S011


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S008


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S006


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S004


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S007


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S005


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S001


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S002


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S003


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S288


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S285


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S287


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S284


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S283


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S282


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S280


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S286


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S012


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S281


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S279


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S278


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S276


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S275


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S273


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S274


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S277


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S272


title: Single-cell RNAseq of lung tumor: T_0w_ND_f1_T0_P1_S074


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S271


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S269


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S267


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S266


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S264


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S262


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S261


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S263


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S258


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S259


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S254


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S257


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S256


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S253


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S255


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S250


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S251


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S249


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S247


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S248


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S270


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S252


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S246


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S245


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S244


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S268


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S241


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S239


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S238


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S265


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S243


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S260


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S235


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S242


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S240


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S237


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S234


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S236


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S233


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S231


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S232


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S228


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S230


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S227


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S229


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S226


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S224


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S220


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S219


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S218


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S221


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S225


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S223


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S212


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S211


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S222


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S207


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S204


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S215


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S214


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S205


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S209


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S216


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S206


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S203


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S210


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S202


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S213


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S208


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S217


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S200


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S198


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S197


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S201


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S196


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S195


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S194


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S193


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S192


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S190


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S189


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S191


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S188


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S186


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S185


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S187


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S183


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S184


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S176


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S177


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S175


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S179


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S182


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S181


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S180


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S178


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S173


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S172


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S169


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S171


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S174


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S168


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S167


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S170


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S166


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S165


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S163


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S162


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S160


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S158


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S159


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S157


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S154


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S152


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S149


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S151


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S148


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S153


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S150


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S146


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S142


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S143


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S144


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S145


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P2_S199


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S139


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S164


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S140


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S138


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S135


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S136


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S137


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S161


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S155


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S133


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S134


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S156


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S147


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S131


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S132


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S130


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S141


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S128


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S126


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S125


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S123


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S120


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S122


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S121


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S118


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S119


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S117


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S114


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S112


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S110


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S113


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S107


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S106


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S105


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S111


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S124


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S116


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S115


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S108


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S109


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S103


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S104


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S127


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S102


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S100


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S101


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S099


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S098


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S384


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S383


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S380


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S378


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S372


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S375


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S377


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S376


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S373


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S371


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S366


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S370


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S369


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S365


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S368


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S367


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S379


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S374


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S360


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S362


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S364


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S361


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S363


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S097


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S357


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S382


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S381


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S359


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S356


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S355


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T9_P1_S129


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S354


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S352


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S348


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S346


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S347


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S344


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S345


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S349


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S340


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S358


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S351


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S335


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S334


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S333


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S341


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S337


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S338


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S331


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S329


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S330


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S328


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S343


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S336


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S326


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S325


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S353


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S350


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S342


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S339


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S322


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S320


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S323


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S321


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S318


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S319


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S316


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S317


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S313


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S312


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S332


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S314


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S309


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S310


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S327


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S306


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S311


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S305


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S303


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S308


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S302


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S304


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S299


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S324


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S301


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S300


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S298


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S315


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S296


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S297


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S307


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S293


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S290


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S295


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S291


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S294


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S382


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S289


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S383


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S384


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S380


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S379


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S377


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S378


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S376


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S375


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S374


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S373


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S370


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S369


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S371


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S366


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P3_S292


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S365


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S364


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S372


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S381


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S368


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S367


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S357


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S359


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S360


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S363


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S362


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S344


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S345


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S361


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S347


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S356


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S343


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S355


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S354


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S351


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S349


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S346


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S342


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S350


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S348


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S353


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S352


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S358


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S339


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S338


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S341


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S340


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S337


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S334


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S336


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S332


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S331


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S329


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S326


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S327


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S323


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S330


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S324


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S322


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S328


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S320


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S316


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S314


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S319


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S321


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S315


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S335


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S317


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S313


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S312


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S311


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S309


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S310


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S307


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S308


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S306


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S333


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S305


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S325


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S318


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S302


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S303


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S301


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S300


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S298


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S297


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S294


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S295


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S299


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S296


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S289


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S291


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S293


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S287


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S286


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S288


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S292


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S290


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S284


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S285


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S283


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S282


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T7_P1_S304


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S280


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S281


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S278


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S279


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S277


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S276


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S275


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S274


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S273


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S271


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S272


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S266


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S268


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S264


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S269


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S267


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S263


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S262


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S260


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S258


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S261


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S254


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S257


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S255


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S259


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S270


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S256


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S248


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S251


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S247


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S245


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S253


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S249


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S252


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S241


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S265


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S242


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S246


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S250


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S244


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S240


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S243


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S239


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S237


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S238


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S235


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S234


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S231


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S233


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S236


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S228


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S229


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S226


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S227


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S232


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S230


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S225


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S223


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S221


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S215


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S219


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S213


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S209


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S216


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S224


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S218


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S208


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S222


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S210


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S212


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S202


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S206


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S201


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S214


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S211


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S200


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S198


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S196


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S205


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S195


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S199


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S220


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S217


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S194


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S193


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S094


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S095


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S092


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S207


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S204


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S096


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S087


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S086


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S203


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S088


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S089


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S084


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P2_S197


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S081


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S080


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S083


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S090


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S091


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S082


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S079


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S085


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S078


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S074


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S075


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S076


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S077


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S073


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S093


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S072


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S070


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S069


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S068


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S071


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S067


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S061


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S057


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S062


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S066


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S063


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S064


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S059


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S060


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S058


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S054


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S055


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S052


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S053


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S051


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S050


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S048


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S049


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S046


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S044


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S047


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S043


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S065


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S042


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S040


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S039


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S037


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S038


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S056


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S036


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S035


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S033


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S029


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S034


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S032


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S030


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S031


title: Single-cell RNAseq of lung tumor: KP_30w_ND_m3_T1_P1_S041


title: A549 Putative CSCs 2, silencing, biological rep2

city: Kepala Batas

first: Badrul

middle: Hisham

last: Yahaya

state: Penang

address: Bertam, Kepala Batas

department: Advanced Medical & Dental Institute

country: Malaysia

institute: Advanced Medical and Dental Institute

description:
Gene expression data from A549 putative CSCs phenotype CD166+CD44+ cells with RARβ gene silencing
A549 CD166+CD44+ RARβ silencing_2

hyb_protocol: Following fragmentation, labelled cRNA were hybridized for 16 hours at 45°C on GeneChip Human Gene 2.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station using the FS450_0002 fluidics protocol .

scan_protocol: GeneChips were scanned using Applied Biosystems™ GeneChip™ Scanner 3000 7G

data_processing: The scanned images were imported into Transcriptome Analysis Console v4.0 software (Thermo Scientific) for array QC. RMA normalization was performed on the samples to generate the quality control (QC) metrics. Microarray data analysis was performed using Affymetrix Transcriptomic Analysis Console (TAC) software (Thermo Scientific).

label: biotin

source_name: A549 cells CD166+CD44+ with RARβ silencing

label_protocol: 100ng of total RNA was reverse transcribed to produce cDNA/mRNA hybrid molecule, which was subsequently used as a template to create double stranded cDNA. This double-stranded cDNA was then amplified via in vitro transcription (IVT) to produce cRNA. In vitro transcription (IVT) generated cRNA was then purified and subjected to 2nd-cycle single-stranded sense cDNA synthesis which was later fragmented, labeled, and hybridized to GeneChip

growth_protocol: Cell (parental, putative CSCs and non-CSCs) with or without silencing were cultured in RPMI 1640 medium supplemented with 10% FBS and 1% antibiotics.

extract_protocol: Total RNA was extracted form cells using Qiagen RNeasy extraction kit according to the manufacturer's instructions.

treatment_protocol: The gene silencing was performed by transfecting Lentiviral carrying shRNA for human RARβ gene (NM_020143). Diluted virus was added into cultured cells and incubated at 37°C  for 6 hours. Then, 1ml of culture medium containing serum was added to each well. Transduced cells was cultured for another 72 hours. After 72 hours, cells were examined under a fluorescence microscope by observing the green fluorescence emitted by the green fluorescent protein (GFP) in the lentivirus particles. GFP+ cell was sorted for RNA extraction.

value: Adenocarcinoma lung cancer cell line


title: A549 Putative non-CSCs 2, silencing, biological rep1

description:
Gene expression data from A549 putative CSCs phenotype CD166-CD44- cells with RARβ gene silencing
A549 CD166-CD44- RARβ silencing_1

source_name: A549 cells CD166-CD44- with RARβ silencing


title: A549 Putative CSCs 1, normal, biological rep1

description:
Gene expression data from A549 putative CSCs phenotype CD166+EpCAM+ cells normal
A549 CD166+EpCAM+ non-silencing_1

source_name: A549 cells CD166+EpCAM+ normal


title: A549 Putative non-CSCs 2, normal, biological rep1

description:
Gene expression data from A549 putative non-CSCs phenotype CD166-CD44- cells normal
A549 CD166-CD44- non-silencing_1

source_name: A549 cells CD166-CD44- normal


title: A549 Putative CSCs 2, normal, biological rep1

description:
Gene expression data from A549 putative CSCs phenotype CD166+CD44+ cells normal
A549 CD166+CD44+ non-silencing_1

source_name: A549 cells CD166+CD44+ normal


title: A549 Putative non-CSCs 1, normal, biological rep2

description:
Gene expression data from A549 putative non-CSCs phenotype CD166-EpCAM- cells normal
A549 CD166-EpCAM- non-silencing_2

source_name: A549 cells CD166-EpCAM- normal


title: A549 Putative non-CSCs 2, normal, biological rep2

description:
Gene expression data from A549 putative non-CSCs phenotype CD166-CD44- cells normal
A549 CD166-CD44- non-silencing_2


title: A549 Putative non-CSCs 2, silencing, biological rep2

description:
Gene expression data from A549 putative CSCs phenotype CD166-CD44- cells with RARβ gene silencing
A549 CD166-CD44- RARβ silencing_2


title: A549 Putative non-CSCs 1, silencing, biological rep1

description:
Gene expression data from A549 putative CSCs phenotype CD166-EpCAM- cells with RARβ gene silencing
A549 CD166-EpCAM- RARβ silencing_1

source_name: A549 cells CD166-EpCAM- with RARβ silencing


title: A549 Putative CSCs 2, silencing, biological rep1

description:
Gene expression data from A549 putative CSCs phenotype CD166+CD44+ cells with RARβ gene silencing
A549 CD166+ECD44+ RARβ silencing_1


title: A549 Parental cells, normal, biological rep1

description:
Gene expression data from A549 parental cells normal
A549 Parental non-silencing_1

source_name: A549 cells


title: A549 Putative CSCs 1, normal, biological rep2

description:
Gene expression data from A549 putative CSCs phenotype CD166+EpCAM+ cells normal
A549 CD166+EpCAM+ non-silencing_2


title: A549 Putative CSCs 1, silencing, biological rep2

description:
Gene expression data from A549 putative CSCs phenotype CD166+EpCAM+ cells with RARβ gene silencing
A549 CD166+EpCAM+ RARβ silencing_2

source_name: A549 cells CD166+EpCAM+ with RARβ silencing


title: A549 Putative CSCs 1, silencing, biological rep1

description:
Gene expression data from A549 putative CSCs phenotype CD166+EpCAM+ cells with RARβ gene silencing
A549 CD166+EpCAM+ RARβ silencing_1


title: A549 Parental cells, silencing, biological rep2

description:
Gene expression data from A549 parental cells with RARβ gene silencing
A549 Parental RARβ silencing_2

source_name: A549 cells with RARβ silencing


title: A549 Parental cells, silencing, biological rep1

description:
Gene expression data from A549 parental cells with RARβ gene silencing
A549 Parental RARβ silencing_1


title: A549 Putative non-CSCs 1, normal, biological rep1

description:
Gene expression data from A549 putative non-CSCs phenotype CD166-EpCAM- cells normal
A549 CD166-EpCAM- non-silencing_1