title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E5_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E4_AD007_indexed

status: Public on Aug 26 2020

type: SRA

city: La Jolla

first: Joseph

middle: R

last: Ecker

state: CA

address: 10010 North Torrey Pines Road

department: Genomic Analysis Laboratory

country: USA

institute: HHMI-Salk-Institute

library_source: genomic

data_processing:
Library strategy: snmC-seq2
Reads trimed with Cutadapt for adapters and low quality bases (Phred score >= 20)
The resulting files are mapped to in-silico bisulfite converted mm10 reference genome using Bismark.
The raw BAM file will be filtered using samtools to filter reads by MAPQ > 10 and use Picard to remove PCR duplicates
Tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls will be generated with in-house developing package using the filtered BAM files
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls. Columns are: 1. Chromosome, 2. Position, 3. Strand, 4. Cytosin Context, 5. methylated base call, 6. total mapped base call, 7. not used, place holder column

molecule: genomic DNA

organism: Mus musculus

source_name: C57BL/6J Male P56 Mouse Brain

growth_protocol: All mice are C57BL/6J Male, brain dissected in P56

extract_protocol:
the brain of P56 male C57BL/6J mouse will be removed and cut into 600 microns thick coronal sections using a tissue matrix. Then each region will be microdissected from the coronal tissue sections based on identified landmarks and brain atlas coordinates, as defined in Allen brain atlas CCF3 (Avaliable at: http://atlas.brain-map.org). For each dissection region, the tissue from several littermate mice will be combined together into to separate replicate groups. The number of animals in a replicate depends on the size of the tissue. Nuclei preparation will be stained with the fluorescently labeled anti-NeuN body and nucleic acid stain Hoechst 33342.
Library prepared following protocol discribed in Luo, Chongyuan, Angeline Rivkin, Jingtian Zhou, Justin P. Sandoval, Laurie Kurihara, Jacinta Lucero, Rosa Castanon, et al. 2018. “Robust Single-Cell DNA Methylome Profiling with snmC-seq2.” Nature Communications 9 (1): 3824.

tag: brain region

value: AI

tag: Sex

value: male

tag: age

tag: strain


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E4_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E5_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E4_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E3_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E3_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E5_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E3_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E3_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E3_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E2_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E4_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E1_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E1_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E1_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E1_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E2_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E2_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E3_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E1_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E2_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E5_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E2_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E1_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E1_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E12_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E1_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E12_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E11_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E12_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E12_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E2_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E3_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E11_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E11_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E12_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_F7_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E11_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E11_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E11_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E11_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E11_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E10_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E10_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E10_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D9_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E10_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D9_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E10_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D9_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D9_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D9_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D7_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E10_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D7_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D7_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D9_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_E10_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D8_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D8_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D8_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D8_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D7_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D8_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D9_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D8_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D7_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D8_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D5_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D7_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D6_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D6_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D6_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D6_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D5_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D6_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D6_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D4_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D9_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D4_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D5_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D3_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D5_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D3_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D3_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D4_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D4_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D5_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D7_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D7_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D6_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D3_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D5_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D3_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D4_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D1_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D4_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D2_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D3_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D2_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D2_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D2_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D2_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D2_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D2_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D3_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D1_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D2_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D1_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D1_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D1_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D1_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D12_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D1_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D12_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D12_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D1_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D12_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D11_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D11_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D11_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D11_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D12_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D10_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D10_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D11_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D11_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D10_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D10_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D10_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C9_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C9_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C9_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D12_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C8_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C8_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D11_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C7_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C7_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D10_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C9_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C8_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C7_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D10_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C8_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C7_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C7_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C9_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_D10_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C6_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C6_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C7_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C9_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C7_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C5_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C6_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C5_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C5_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C5_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C5_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C6_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C5_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C6_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C7_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C4_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C4_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C4_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C4_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C4_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C5_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C4_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C2_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C3_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C6_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C3_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C3_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C4_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C3_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C2_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C3_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C4_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C2_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C2_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C3_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C3_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C2_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C3_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C1_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C2_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C1_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C1_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C12_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C1_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C12_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C12_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C2_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C1_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C1_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C12_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C11_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C12_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C12_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C11_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C11_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C12_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C11_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C10_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C10_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C11_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C11_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C1_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C10_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C1_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B9_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C10_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B9_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B9_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B9_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B9_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B9_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C10_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B8_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B9_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B8_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B8_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B8_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C10_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B8_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B8_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B7_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B7_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B6_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B7_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B9_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_C10_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B6_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B7_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B6_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B6_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B7_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B6_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B8_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B6_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B6_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B5_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B6_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B8_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B5_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B7_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B3_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B4_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B5_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B5_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B4_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B4_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B5_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B5_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B4_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B5_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B4_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B4_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B3_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B3_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B2_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B7_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B5_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B3_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B2_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B2_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B2_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B2_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B1_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B3_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B1_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B2_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B1_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B1_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B12_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B4_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B1_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B12_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B12_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B12_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B11_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B12_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B12_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B11_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B11_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B11_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B11_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B12_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B11_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B11_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B11_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B2_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B10_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B10_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B1_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B10_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A9_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A9_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A9_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B10_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B2_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A9_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B12_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B10_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A9_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A9_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B10_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A8_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A8_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A8_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A8_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B10_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A7_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A7_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_B10_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A8_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A8_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A7_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A7_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A7_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A9_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A7_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A6_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A7_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A6_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A6_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A5_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A6_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A6_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A7_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A5_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A5_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A6_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A5_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A6_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A4_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A5_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A4_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A4_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A3_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A4_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A3_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A4_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A4_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A5_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A3_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A3_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A3_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A3_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A2_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A6_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A12_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A2_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A2_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A2_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A2_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A2_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A2_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A2_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A1_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A1_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A1_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A3_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A1_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A1_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A11_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A12_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A12_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A10_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A10_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A10_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A12_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A3_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A12_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A10_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A10_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A11_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A10_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A11_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G1_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F2_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A11_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A12_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_7_CEMBA180412_3D_8_G4_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_7_CEMBA180412_3D_8_D3_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_7_CEMBA180412_3D_8_A12_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_7_CEMBA180412_3D_8_A4_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_7_CEMBA180412_3D_8_A1_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_7_CEMBA180412_3D_8_A2_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H8_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_7_CEMBA180412_3D_8_A10_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_7_CEMBA180412_3D_8_A1_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_7_CEMBA180412_3D_8_A10_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180416_3D_5_CEMBA180416_3D_6_A1_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H9_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H9_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H9_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H8_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H9_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H8_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H8_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H8_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H7_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H7_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_7_CEMBA180412_3D_8_A2_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_7_CEMBA180412_3D_8_A11_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H7_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H7_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_7_CEMBA180412_3D_8_A11_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_7_CEMBA180412_3D_8_A12_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H7_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H6_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H6_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H6_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H6_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H6_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H5_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H5_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H5_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H9_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H7_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H6_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H4_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H4_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H5_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H4_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H5_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H4_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H5_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H4_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H3_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H3_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H3_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H3_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H3_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H3_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H2_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H2_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H2_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H2_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H1_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H2_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H2_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H1_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H1_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H1_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H12_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H12_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H12_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H10_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H12_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H10_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H11_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H10_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H10_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G9_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H11_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G9_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G9_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G9_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H10_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H12_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H1_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G9_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H1_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H12_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G8_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G8_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G8_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G9_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G9_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H11_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H12_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G7_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G7_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G7_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_H10_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G8_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G7_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G7_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G7_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G6_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G6_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G6_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G6_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G6_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G5_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G5_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G5_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G4_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G5_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G4_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G5_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G6_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G4_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G4_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G4_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G3_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G4_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G3_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G3_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G3_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G3_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G2_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G2_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G3_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G6_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G2_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G2_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G2_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G2_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G1_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G1_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G1_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G1_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G12_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G12_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G12_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G12_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G11_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G11_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G12_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G1_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G11_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G11_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G12_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G11_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G11_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G10_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G10_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G10_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G12_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G1_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G10_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F9_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F9_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F9_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F9_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F9_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G11_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G11_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F8_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F8_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F8_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F9_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F8_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F8_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F8_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F8_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F7_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F7_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F7_AD010_indexed


title: s18: Liver - M_PxrWT_ATB - Rep6

type: RNA

city: Toulouse

first: Sandrine

last: Ellero-Simatos

address: 180 chemin de Tournefeuille

country: France

institute: INRAe

hyb_protocol: 600 ng of Cy3-labelled cRNA (specific activity >6 pmol Cy3/µg cRNA) was fragmented at 60°C for 30 minutes in a reaction volume of 25 µl containing 10x Agilent fragmentation buffer and 25x Agilent blocking agent following the manufacturer's instructions (Agilent Technologies, Santa Clara, CA). On completion of the fragmentation reaction, 25 µl of 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to SurePrint G3 Mouse GE v2 microarray (8X60K, Design 074809  enclosed in Agilent SureHyb-enabled hybridization chambers for 17 hours at 65°C in a rotating Agilent hybridization oven. After hybridization, microarrays were washed sequentially in Wash buffer 1 (Agilent Technologies, 1 min) and Wash buffer 2 (Agilent Technologies, 37°C, 1 min).

scan_protocol: Slides were scanned immediately after washing on a Agilent G2505C Microarray Scanner with Agilent Scan Control A.8.5.1 software

data_processing: The scanned images were analyzed with Feature Extraction Software 10.10.1.1 (Agilent Technologies, Santa Clara, CA) using default parameters (protocol GE1_1010_Sep10 and Grid: 074809_D_F_20150624). All subsequent data analyses were done under R (www.r-project.org) using packages of Bioconductor (www.bioconductor.org). Raw data (median of pixels intensity) were imported into R using the read.maimages function from the limma package with the following weight function (assigning a weight of 1 or 0 to each spot): myfunw<-function(x) {okType<-x$ControlType==0; okFoundGreen<-x$gIsFound==1; okPos=x$gIsPosAndSignif==1; okWellAbove<- x$gIsWellAboveBG==1; as.numeric(okType & okFoundGreen & okPos & okWellAbove);} We selected the spots with a minimal weight of 1 for 39 out of 48 microarrays or with a minimal weight of 4 per group from at least one experimental group. At this step, 36041 spots out of 62976 were selected.  The mean signal of the 24 first samples, 16 next and 8 last ones were substracted to individuals signals to correct the batch effect of the 3 serials (characteristics:WashBatch) observed during the microarray washing procedure. Data were then stored in an ExpressionSet object and normalized by the qsmooth method using the qsmooth function from the qsmooth R library. Replicated probes on the array (identical ProbeName) were resolved by taking the median normalized signal of each set of replicated probes. The resulting matrix has 33383 rows each corresponding to a unique ProbeName (provided as data Matrix).

molecule: total RNA

source_name: Liver

label_protocol: For each sample, Cyanine-3 (Cy3) labeled cRNA was prepared from 200 ng of total RNA using the One-Color Quick Amp Labeling kit (Agilent Technologies, Santa Clara, CA) according to the manufacturer's instructions, followed by Agencourt RNAClean XP (Agencourt Bioscience Corporation, Beverly, Massachusetts) purification. Dye incorporation and cRNA yield were checked using Dropsense™ 96 UV/VIS droplet reader (Trinean, Belgium).

growth_protocol: All mice were housed at 21–23°C on a 12-h light (ZT0–ZT12)/ 12-h dark (ZT12–ZT24) cycle and allowed free access to the diet (Teklad Global 18% Protein Rodent Diet) and tap water. ZT stands for Zeitgeber time; ZT0 is defined as the time when the lights are turned on. Pxr-/- animals (backcrossed on the C57Bl/6J background) were originally engineered in Pr. Meyer’s laboratory (Staudinger et al., 2001) and have been bred for 10 years in our animal facility.

extract_protocol: Following euthanasia by cervical dislocation, liver tissue was weighed, snap-frozen in liquid nitrogen and stored at -80°C until used. Total RNA was extracted using TRI Reagent® (Molecular Research Center, Cincinnati, Ohio, USA). RNAs were quantified using Nanodrop (NanoDropTM 1000; Thermo Scientific).

treatment_protocol: Pxr+/+ and Pxr-/- littermate mice were derived from Pxr+/- dams and separated by sex and genotype at 5 weeks. Mice were randomly allocated to the different experimental groups (n=8 in 2 cages per group). Three antibiotics were dissolved in tap water and were provided in the animal bottles for 2 weeks: ampicillin (1g/L), neomycin (1 g/L) and vancomycin (0.5 g/L). The antibiotic solutions were prepared fresh every 3 days.  Mice were sacrificed at ZT6.

tag: gender

value: M

tag: genotype

value: PxrWT

tag: tissue

value: Liver

tag: treatment

value: ATB

tag: washbatch


title: s25: Liver - M_PxrKO_CTRL - Rep6

value: PxrKO

value: CTRL


title: s59: Liver - F_PxrKO_CTRL - Rep5

value: F


title: s9: Liver - M_PxrWT_CTRL - Rep6


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F7_AD008_indexed


title: s66: Liver - F_PxrKO_ATB - Rep4


title: s40: Liver - F_PxrWT_CTRL - Rep5


title: s50: Liver - F_PxrWT_ATB - Rep6


title: s24: Liver - M_PxrKO_CTRL - Rep5


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F7_AD004_indexed


title: s8: Liver - M_PxrWT_CTRL - Rep5


title: s60: Liver - F_PxrKO_CTRL - Rep6


title: s6: Liver - M_PxrWT_CTRL - Rep4


title: s49: Liver - F_PxrWT_ATB - Rep5


title: s41: Liver - F_PxrWT_CTRL - Rep6


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F7_AD006_indexed


title: s68: Liver - F_PxrKO_ATB - Rep5


title: s15: Liver - M_PxrWT_ATB - Rep4


title: s16: Liver - M_PxrWT_ATB - Rep5


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F6_AD001_indexed


title: s33: Liver - M_PxrKO_ATB - Rep5


title: s39: Liver - F_PxrWT_CTRL - Rep4


title: s45: Liver - F_PxrWT_ATB - Rep2


title: s34: Liver - M_PxrKO_ATB - Rep6


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F6_AD012_indexed


title: s57: Liver - F_PxrKO_CTRL - Rep4


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F7_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F6_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F6_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F5_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F6_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F6_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F5_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F6_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F7_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F5_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F5_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F5_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_G8_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F4_AD010_indexed


title: s23: Liver - M_PxrKO_CTRL - Rep4


title: s65: Liver - F_PxrKO_ATB - Rep3


title: s38: Liver - F_PxrWT_CTRL - Rep3


title: s70: Liver - F_PxrKO_ATB - Rep6


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F4_AD004_indexed


title: s1: Liver - M_PxrWT_CTRL - Rep1


title: s14: Liver - M_PxrWT_ATB - Rep3


title: s55: Liver - F_PxrKO_CTRL - Rep3


title: s64: Liver - F_PxrKO_ATB - Rep2


title: s46: Liver - F_PxrWT_ATB - Rep3


title: s22: Liver - M_PxrKO_CTRL - Rep3


title: s28: Liver - M_PxrKO_ATB - Rep2


title: s27: Liver - M_PxrKO_ATB - Rep1


title: s12: Liver - M_PxrWT_ATB - Rep2


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F4_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F5_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F3_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F4_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F3_AD004_indexed


title: s36: Liver - F_PxrWT_CTRL - Rep2


title: s54: Liver - F_PxrKO_CTRL - Rep2


title: s48: Liver - F_PxrWT_ATB - Rep4


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F4_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F4_AD001_indexed


title: s2: Liver - M_PxrWT_CTRL - Rep2


title: s21: Liver - M_PxrKO_CTRL - Rep2


title: s62: Liver - F_PxrKO_ATB - Rep1


title: s53: Liver - F_PxrKO_CTRL - Rep1


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F3_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F3_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F2_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F2_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F5_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F3_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F3_AD010_indexed


title: MM.1S BR-SI2-Input

city: Tianjin

first: Liu

last: Zhiqiang

address: 22 Qxiangtai Rd

country: China

institute: Tianjin Medical University

data_processing:
Basecalls performed using HiSeqX
ChIP-seq reads were aligned to the hg19 genome assembly using BWA version 0.7.13 with the following configurations -q 5 -l 32 -k 2
Data were filtered using Picard-tools version 1.126
peaks were called using macs2 version 2.1.0 with the following setting: -pvalue 0.01 --keep-dup all --extsize -1 --shift 0
Genome_build: hg19
Supplementary_files_format_and_content: bigwig files were generated using macs2; Scores represent an average read coverage
Supplementary_files_format_and_content: narrowpeak files

organism: Homo sapiens

growth_protocol: MM.1S BR cell was cultured in RPMI-1640 medium with 10% FBS.

extract_protocol:
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody.
Libraries were prepared according to the TruePrep® DNA Library Prep Kit V2 for Illumina (TD503-02). Briefly, TruePrep Tagment Enzyme Mix (TTEMix) contains transposase and two kinds of adapters (Adapter 1 and Adapter 2) with equal molar. Input DNA are fragmented and linked with adapters on both ends just by mixing with TTE Mix, followed by a 10-minute incubation at 55℃. The tagged DNA fragments can be further amplified with two pairs of primers N5 (N5XX) / N7 (N7XX) and P5 / P7 (PCR Primer Mix, PPM).  After adapter ligation DNA was PCR amplified with Illumina primers for 15 cycles and library fragments of ~250 bp (insert plus adaptor and PCR primer sequences) were band isolated from an agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Illumina HiSeq X following the manufacturer's protocols.

treatment_protocol: MM.1S BR cell was 25nM SI-2 treated for 24 hours.

tag: cell line

tag: cell type

value: B lymphoblast

tag: tumor

value: 42 years black female immunoglobulin A lambda myeloma

tag: chip antibody

value: treated with SI-2 for 24 hours


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F2_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F2_AD001_indexed


title: MM.1S BR-SI2- H3K36me2-IP

value: treated with SI2 for 24 hours


title: MM.1S BR-H3K36me2-IP

value: untreated


title: MM.1S BR-SI2-Pol II-IP

value: Rpb1 NTD(Cell signaling technology, 14958, lot:1)


title: MM.1S BR-Pol II-IP


title: DipG3

city: Nanjing

first: Minmin

last: Fan

state: Jiangsu

address: 138 Xianlin Avenue

institute: Nanjing University of Chinese Medicine

library_source: transcriptomic

data_processing:
Raw data (raw reads) were processed using Trimmomatic. The reads containing poly-N and the low quality reads were removed to obtain the clean reads. Then the clean reads were mapped to reference genome using hisat2.
FPKM value of each gene was calculated using cufflinks, and the read counts of each gene were obtained by htsep-count.
DEGs were identified using the DESeq (2012) R package functions estimateSizeFactors an nbinomTest.
Genome_build: GRCh38.p12
Supplementary_files_format_and_content: Text files include FPKM values for each Sample.

source_name: MDA-MB-231_diptoindonesin G

growth_protocol: MDA-MB-231 cells were cultured in DMEM medium containing 10% fetal bovine serum.

extract_protocol:
Total RNA was extracted using the mirVana miRNA Isolation Kit (Ambion) following the manufacturer’s protocol.
The libraries were constructed using TruSeq Stranded mRNA LTSample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions.

treatment_protocol: The cells were incubated for 24 hours in the absence or presence of 15 μM diptoindonesin G.

value: triple negative breast cancer cell line

value: diptoindonesin G (15 μM)


title: s43: Liver - F_PxrWT_ATB - Rep1


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F2_AD007_indexed

value: AI


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F1_AD012_indexed

value: male


title: DipG2


title: s11: Liver - M_PxrWT_ATB - Rep1

value: M


title: s35: Liver - F_PxrWT_CTRL - Rep1

value: PxrWT


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F1_AD004_indexed


title: s3: Liver - M_PxrWT_CTRL - Rep3

value: Liver


title: MM.1S BR-Input


title: DipG1


title: NC3


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F1_AD008_indexed


title: s30: Liver - M_PxrKO_ATB - Rep3

value: ATB


title: NC2


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F1_AD002_indexed


title: NC1


title: CTRL2_CT0.4_D8

city: Stanford

first: Zhuoqing

last: Fang

state: California

address: 300 Pasteur Drive

institute: Stanford

data_processing:
Basecalls performed using CASAVA version 1.7
Sequences are aligned to transcriptome with Salmon (v0.8.2)
Transcripts Per Million were calculated by Salmon(v0.8.2)by defalut parameters
Genome_build: hg38
Supplementary_files_format_and_content: comma seprated file(.csv) include TPM values for all samples

molecule: polyA RNA

source_name: Neural progenitor cells

extract_protocol:
Total RNA was isolated from cells collected at different time points using TRIzol Reagent (Invitrogen). Isolated RNA was enriched by Poly(A) tails and fragmented. Sequencing libraries were prepared according to Illumina manufacturer instruction. Paired-end RNA-seq of 2x100 base pair reads were sequenced on Illumina Hiseq2000 platform or 2x150 base pair reads were sequenced on Hiseq X-ten
RNA libraries were prepared for sequencing using standard Illumina protocols

value: human ES-derived neural progenitor cells

value: wild type


title: CTRL2_CT1.0_D8


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F12_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F1_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F12_AD008_indexed


title: CTRL1_CT1.0_D8


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F12_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F12_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F12_AD001_indexed


title: CTRL2_IWP2_D8


title: CTRL1_IWP2_D8


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F11_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F11_AD004_indexed


title: CTRL2_CT1.0_D4


title: CTRL2_CT0.4_D4


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F1_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F11_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F11_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F11_AD002_indexed


title: CTRL2_IWP2_D4


title: CTRL1_IWP2_D4


title: CTRL1_CT1.0_D4


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F10_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F10_AD008_indexed


title: S1KO48_CT1.0_D8


title: S1KO26_CT1.0_D8


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F10_AD006_indexed


title: CTRL1_CT0.4_D8


title: S1KO48_CT0.4_D8


title: S1KO26_CT0.4_D8


title: S1KO48_IWP2_D8


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E9_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F10_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_F10_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E9_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E9_AD012_indexed


title: S1KO48_CT1.0_D4


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E8_AD012_indexed


title: S1KO26_IWP2_D8


title: S1KO48_CT0.4_D4


title: CTRL1_CT0.4_D4


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E9_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E8_AD008_indexed


title: S1KO48_IWP2_D4


title: S1KO26_IWP2_D4


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E7_AD008_indexed


title: S1KO48_ES

source_name: Human embryonic stem cell

value: human embryonic stem cell


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E8_AD010_indexed


title: S1KO26_CT0.4_D4


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E8_AD006_indexed


title: S1KO26_ES


title: CTRL2_ES (SOX1)


title: CTRL1_ES (SOX1)


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E8_AD002_indexed


title: S1KO26_CT1.0_D4


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E8_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E7_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E8_AD007_indexed


title: B5_Expression

city: Guangzhou

first: Yuxia

last: Zhang

state: Guangdong

address: 9 Jinshui Rd, Tianhe District

institute: Guangzhou Women and Children's Medical Center

description: Single cell 5' expression library

data_processing:
Samples were demultiplexed using Illumina bcl2fasq software
The Cell Ranger v2.1.1 pipeline (10x Genomics) was utilized to align reads to the transcriptome and summarize counts
The unique molecular identifier (UMI) count matrix was converted to a Seurat object using the R package Seurat v2.3.4. During this process, only cells with at least 200 genes detected and genes expressed in at least 3 cells were retained. The raw UMI counts matrix was then normalized using the Seurat’s NormalizeData function, in which the number of UMIs of each gene was divided by the sum of total UMIs per cell and multiplied by 10000, then transformed to log-scale (In(UMI-per-10000+1)).
hg38
tab-delimited raw gene expression matrix (UMI counts)
tab-delimited normalized gene expression matrix (In(UMIs-per-10000+1))

source_name: BALF

extract_protocol:
The BALF samples were filtered with double-layer gauze and wash tiwce with PBS containing 0.5% FBS and 0.5mM EDTA. Then the cell pellets were mixed and stained with mutiple antibody combinations (CD45 PE dazzle594, CD14 FITC, CD16 FITC, CD3 BV711) for 30 mins before wash with PBS containing 0.5% FBS and 0.5mM EDTA. Immune cells were sorted by FACS Aria Sorp system.
Single cell solution (viability >90%, 1000 cells/uL) was prepared and partitioned into nanoliter-scale Gel Bead-In-EMulsions (GEMs). This would result with expected mRNA barcoding for ~5000 single-cells and multiplet rate of ~3.9%. After reverse transcription, GEMs were broken and barcoded-cDNA purified with Silane magnetic beads and Solid Phase Reversible Immobilization (SPRI) beads. Barcoded-cDNA was amplified by PCR (98ºC for 45s; [98ºC for 20s, 67ºC for 30s, 72ºC for 1 min] x 14; 72ºC for 1 min).The cDNA was splited into two subsamples subjected to library construction according to the instructions of Chromium Single Cell 5' library and TCR- or BCR-enriched library,respectively.

tag: patient origin


title: B5_BCR

description: Single cell 5' expression library

data_processing:
Samples were demultiplexed using Illumina bcl2fasq software
The fastq files were token to peform V(D)J sequence assembly and paired clonotype calling with the cellranger vdj pipeline  (Cell Ranger v2.1.1,10x Genomics).cellranger vdj used the Chromium cellular barcodes and UMIs to assemble  V(D)J transcripts cell-by-cell.
hg38
5prime_vloupe_TCR.vloupe or 5prime_vloupe_BCR.vloupe


title: T5_TCR

description: Single cell 5' expression library


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E7_AD006_indexed


title: T5_Expression

description: Single cell 5' expression library


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E9_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E6_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E6_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E7_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E6_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E9_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E5_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E5_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E5_AD004_indexed


title: s31: Liver - M_PxrKO_ATB - Rep4

value: PxrKO


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E6_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E4_AD004_indexed


title: ENR_STD5

city: Dresden

first: Gerd

last: Kempermann

state: Saxony

address: Tatzberg 41

country: Germany

institute: German Center for Neurodegenerative Diseases (DZNE)

description: Methylated cytosines converted to uracil by treatment with sodium bisulfite

data_processing:
Trimming: Fastq reads were trimmed using Trim Galore 0.4.0 and the function Cutadapt 1.8.1. To remove cytosines that were filled in during ends preparation, an additional 2 bp were cut off from every sequence with detected adapter.
Alignment: Trimmed reads were mapped against mm10 using Bismark 0.16.1
Base-calling: MethylKit 1.5.2.
Genome_build: mm10
Supplementary_files_format_and_content: Methylation percentages of cytosines for individual samples. Txt file exported from Methylkit.

growth_protocol: Mice housed in 12:12 light:dark cycle under SPF conditions and given ad libitum access to mouse chow and water.

extract_protocol:
Micro-dissection of the hippocampal dentate gyrus
Genomic DNA was isolated using the QIAamp DNA Micro Kit (QIAGEN). RRBS libraries were prepared using the Premium RRBS Kit (Diagenode) and purified twice using Agencourt AMPure XP beads (Beckman Coulter; 1X bead volume). Quality and concentration of RRBS libraries were determined using the High Sensitivity NGS Fragment Analysis Kit (Advanced Analytical) and a fragment analyzer with capillary size of 33 cm (Advanced Analytical).

treatment_protocol: Mice were housed in either standard cages or in an enriched environment.

value: Dentate gyrus

value: 7.5 months

value: Female

tag: housing

value: Three months of environmental enrichment followed by three months of standard housing


title: ENR_STD2


title: ENR_STD4


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E5_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E5_AD010_indexed


title: ENR_STD3


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E4_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E7_AD007_indexed


title: ENR_STD1


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E4_AD001_indexed


title: ENR5

value: Environmental enrichment


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E3_AD010_indexed


title: ENR4


title: ENR3


title: ENR2


title: ENR1


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E3_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E3_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E3_AD008_indexed


title: STD5

value: Standard housing


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E3_AD006_indexed


title: STD4


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E3_AD002_indexed


title: STD2


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E4_AD002_indexed


title: STD1


title: STD3


title: M2_IL17AF

city: Iowa City

first: Nitin

last: Karandikar

state: Iowa

address: C673 General Hospital

department: Pathology

institute: University of Iowa

data_processing:
Single-end 50 bp sequencing was performed on the Illumina HiSeq 2000. Basecalls were converted into FASTQs using the Illumina bcl2fastq and alignment was performed using kallisto  with the GRCh38 human genome build.
Genome_build: GRCh38
Supplementary_files_format_and_content: tab-delimited tsv files including lengths, counts and transcripts per million (TPM) for mRNA transcripts

source_name: CD4+CD25- T-cells

growth_protocol: Ex vivo bulk CD4+CD25- T-cells were thawed in RPMI 1640 (Corning 10-040-CV) with DNase at 10KU/ml (Sigma D4513-1vl) and then resuspended at 1x10e6 cells/ml in X-VIVO 15 serum-free media (Lonza, 04-418Q)

extract_protocol: Samples were submitted to the University of Chicago Genomics facility for RNA extraction, quality assessment and sequencing

treatment_protocol: Cells were incubated 60 minutes in vitro in the presence of media alone (controls), or 10 ng/ml of recombinant human cytokine IL17A (eBioscience, 34-8179-82), IL17F (R&DSystems, 1335-IL-025/CF) or IL17A + IL17F.  Conditions were activated with 1 ug/ml each of fixed anti-CD3 (eBioscience, 16-0037-85) and anti-CD28 (eBioscience, 16-0289-85) and incubated for 48 hours.  Cultured cells were pelletized, snap frozen in liquid nitrogen, and stored at -80 C for shipment to Chicago for rna extraction and sequencing

tag: condition

value: ILL17A 10 ng/ml +IL17F 10 ng/ml

tag: sample


title: M3_C

value: No cytokine added control


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E2_AD002_indexed

tag: brain region

value: AI

value: male


title: M3_IL17F

value: IL17F 10 ng/ml


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E4_AD007_indexed

tag: Sex


title: M3_IL17AF


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E3_AD001_indexed

tag: age


title: M2_IL17F


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E2_AD010_indexed

tag: strain


title: M1_IL17AF


title: M1_IL17F


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E3_AD004_indexed


title: M2_IL17A

value: IL17A 10 ng/ml


title: M2_C


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E2_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E2_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E12_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E12_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E1_AD008_indexed


title: M1_IL17A


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E1_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E1_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E2_AD008_indexed


title: M3_IL17A


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E2_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E12_AD008_indexed


title: M1_C


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E1_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E12_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E12_AD010_indexed


title: NB4_Input_1

city: Shanghai

first: Yun

last: Tan

state: Shanghai

address: Room 1109, Building 11, 197 Ruijin Er Rd

department: Shanghai Institute of Hematology

institute: Ruijin Hospital, Shanghai Jiao Tong University School of Medicine

data_processing:
Sequencing reads were aligned to hg38 by Bowtie2 (version 2.2.9). The uniquely mapped and non-redundant reads were left to conduct peak calling.
ChIP-seq peak regions were called using MACS (version 1.4.2) at a p-value cut off of 10e-10. NB4_Input1 was used as control for NB4_ChIP_seq_HDAC1, NB4_ChIP_seq_P300 and NB4_ChIP_seq_PML_RARA, while NB4_Input2 was used as control for NB4_ChIP_seq_H3K27ac
Genome_build: hg38
Supplementary_files_format_and_content: peak.xls files were generated by MACS software

source_name: Acute promyelocytic leukemia cells

growth_protocol: NB4 cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) in a humidified atmosphere with 5% CO2 at 37°C.

extract_protocol:
Cells were washed once with PBS buffer and 3 times with lysis buffer (10 mM Tris-HCl, PH 7.5, 10 mM NaCl, 3 mM MgCl2, 0.5% NP-40, 1 mM PMSF). The pellets were then re-suspended in ChIP buffer (Active motif) containing 1 mM PMSF and proteinase inhibitor cocktail (Sigma). Chromatin shearing was performed using a Biorupter Pico ultrasonicator device (Diagenode) with 30s ON then 30s OFF for 30 cycles. Solubilized chromatin was used for immunoprecipitation by incubating overnight at 4 °C with 5 μg antibodies against PML/RARα fusion protein. The immunoprecipitation reaction was transferred to the Protein G agarose Column (53039, Active Motif) and incubated for 2 hours at 4 °C, then each column was washed 4 times with 900 μL Wash Buffer AM1 (Active motif). The ChIPed sample was eluted using 100 μL 37 °C Elution Buffer AM4 (Active Motif). Cross-linking was reversed by adding 5 μL Proteinase K and the sample was incubated overnight at 65 °C. Input and ChIPed DNA were purified using a DNA purification column (Active Motif).
DNA libraries were prepared with the MicroPlex Library Preparation Kit (Diagenode) according to the manufacturer’s instructions.

treatment_protocol: NB4 cells were cross-linked with 1% formaldehyde in Phosphate Buffered Saline (PBS) buffer for 10min at room temperature, and the fixation reactions were quenched by adding glycine to a final concentration of 0.125 M.

tag: cell lne

value: Acute promyelocytic leukemia (APL) patient-derived cell line


title: NB4_ChIP_seq_P300

value: anti-P300 (Santa Cruz Biotechnology, sc-585x, Lot J2214)


title: NB4_ChIP_seq_H3K27ac


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E11_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E12_AD007_indexed


title: NB4_ChIP_seq_HDAC1

value: anti-HDAC1 (Millipore, 06-720, Lot 2571576)


title: NB4_ChIP_seq_PML_RARA

value: anti-PML/RARa (customized by ABclonal Biotechnology)


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E1_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E10_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E10_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E11_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D9_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D9_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E11_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E10_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E10_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E11_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D8_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D9_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D8_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D8_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D8_AD007_indexed


title: NB4_Input_2


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D8_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E10_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D7_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D9_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D8_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D7_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_E10_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D9_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D7_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D7_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D6_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D6_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D7_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D6_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D7_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D6_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D6_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D6_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D5_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D5_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D7_AD007_indexed


title: Male6_DMSO input

city: St. Louis

first: Zongtai

last: Qi

state: Missouri

address: 4515 Mckinley Ave

department: Genetics

institute: Washington University in St Louis

data_processing:
ChIP-seq reads were aligned to the murine genome build mm10 using Bowtie2  (version 2.3.4.3) and only uniquely aligning reads were used  for downstream analyses.
Regions of enrichment of H3K27ac over background were calculated using the MACS version (2.1.0) peak finding algorithm. An adjusted p-value threshold of enrichment of 0.01 was used for all data sets. The resulting peak files were used as inputs for DiffBind (version 3.5) to derive consensus peak sets.
The differential enrichment of H3K7Ac signals between male and female analysis was carried out with Diffbind using DESeq2 (method = DBA_DESEQ2) with libraries normalized to total library size.

source_name: Astrocytes

growth_protocol: Cells were grown in DMEM/F12 media supplemented with 10% FBS and 1% penicillin-streptomycin. These astrocytes serve as a model of GBM and we refer to them as male and female GBM astrocytes.

extract_protocol:
Briefly, cells were fixed in 1% formaldehyde prepared in DMEM/F12 and incubated for 10 minutes at room temperature with gentle rocking.  Fixation was quenched by the addition of glycine to a final concentration of 125 mM and incubation for 10 minutes at room temperature with gentle rocking. Cells were then washed with cold PBS and harvested by scraping in 5 ml of cold PBS.  Cells were transferred to 15 mL conical tubes and pelleted with a 5-minute spin (1,000g) at 4ºC. Sonication was performed on an Epishear probe-in sonicator (Active Motif) at 40% amplitude for 8 cycles of 30 seconds with 30 seconds of rest between each cycle.
ChIP-seq libraries were constructed as previously described, with an antibody that recognizes H3K27Ac (Active Motif, 39133, rabbit polyclonal). ChIP-seq libraries were constructed with sample-specific barcodes, and pooled before sequencing on a HiSeq 2500 (Illumina).

treatment_protocol: GBM cells were treated with 0.05% DMSO or 500 nM JQ1 for 24 hours. Following treatment, cells were then prepared for various downstream analyses including ChIP-seq for H3K27ac.

tag: antibody

value: Male

tag: genotype

value: null for Nf1 and p53

value: astrocytes

value: DMSO


title: Female6_DMSO input

value: Female

tag: treatment


title: Male6_JQ1 input


title: Female6_JQ1 input


title: Female6_JQ1 rep3


title: Female6_JQ1 rep2


title: Female6_DMSO rep3


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D5_AD002_indexed


title: Female6_DMSO rep2


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D6_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D5_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D4_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D4_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D4_AD012_indexed


title: Female6_DMSO rep1


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D3_AD010_indexed


title: Male6_JQ1 rep2


title: Male6_JQ1 rep1


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D5_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D4_AD004_indexed


title: Male6_JQ1 rep3


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D3_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D3_AD008_indexed


title: Male6_DMSO rep2


title: Female6_JQ1 rep1


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D5_AD001_indexed


title: Male6_DMSO rep1


title: Female6_cpi_rep1

data_processing:
RNA-seq data sets were aligned to the transcriptome and the whole-genome with STAR (version 2.7.0)
Genes or exons were filtered for just those that were expressed. The read-count tables for annotated genes in the mm10 gene transfer format (.GTF) file were derived from uniquely aligned reads using HTSeq (version 0.11.1)
The raw counts for each gene were converted into FKBP or TPM for comparisons of gene expression levels between samples.

extract_protocol:
Total RNA was isolated from male and female GBM cells that were treated with DMSO (0.05%) or JQ1 (500 nM for 24 hours) or CPI0610 (1µM for 24 hours) using the RNeasy Mini Kit from QIAGEN (Hilden, Germany), following the kit protocol. PolyA Selection was performed to create RNA Seq libraries. Cell mRNA was extracted from total RNA using a Dynal mRNA Direct kit. The quantity of RNA was measured using a spectrophotometer (NanoDrop 2000c; Thermo Scientific). Samples with an RNA concentration A260/A280 ≥1.8 ng/μl, and purity A230/A260 ≥ 2.0 ng/μl were selected. The Agilent 2100 Bioanalyzer was used to determine the RNA integrity number. The degradation level was identified using the RNA 6000 Nano LabChip kit (Agilent).
Samples with RNA integrity number > 9.8 were further processed using TruSeq mRNA Library Preparation Kit (Illumina) and then sequenced on a HiSeq 25000 (Illumina).

treatment_protocol: GBM cells were treated with 0.05% DMSO or 500 nM JQ1 for 24 hours. Following treatment, cells were then prepared for various downstream analyses including RNA-seq.

value: Astrocytes


title: Female6_cpi_rep3


title: Female6_cpi_rep2


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D3_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D2_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D2_AD004_indexed


title: Female6_dmso_2_rep3


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D2_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D3_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D2_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D2_AD002_indexed


title: Female6_dmso_2_rep1


title: Male6_cpi_rep3


title: Female6_dmso_2_rep2


title: Male6_DMSO rep3


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D2_AD001_indexed


title: Male6_cpi_rep2


title: Male6_cpi_rep1


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D12_AD008_indexed


title: Male6_dmso_2_rep2


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D12_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D12_AD012_indexed


title: Male6_dmso_2_rep1


title: Male6_dmso_2_rep3


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D4_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D12_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D1_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D12_AD002_indexed


title: Female6_jq1_rep2


title: Female6_jq1_rep3


title: Female6_dmso_1_rep3


title: Female6_jq1_rep1


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D12_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D11_AD012_indexed


title: Female6_dmso_1_rep2


title: Male6_jq1_rep3


title: Male6_jq1_rep2


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D11_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D11_AD007_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D11_AD008_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D10_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D11_AD004_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D12_AD004_indexed


title: Male6_jq1_rep1


title: Male6_dmso_1_rep1


title: Male6_dmso_1_rep2


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D11_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D10_AD006_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D11_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D10_AD010_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D10_AD008_indexed


title: Female6_dmso_1_rep1


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D10_AD007_indexed


title: memory P14 T cells 12 days post-lymphocytic choriomeningitis virus infection

city: Minneapolis

first: Todd

middle: P

last: Knutson

state: MN

address: 117 Pleasant St SE

department: Minnesota Supercomputing Institute

institute: University of Minnesota

description:
scRNAseq_Day12_P14
single cell mRNA
experiment_2_singlecell_rnaseq_day12_day60_norm_counts.txt
experiment_2_singlecell_rnaseq_day12_day60_raw_counts.txt

data_processing:
Illumina bcl2fastq (bulk RNAseq) or 10X Genomics Cell Ranger (scRNAseq) software was used to demultiplex samples and generate fastq files.
Read mapping was performed via Hisat2 (Bulk RNAseq) or Cell Ranger count (scRNAseq) using the mouse reference genome (mm10).
Gene level quantification was completed using Subread featureCounts (bulk RNASeq) or Cell Ranger count software.
Raw counts data was imported into R for downstream processing, including normalization, principal components analysis, differential gene expression testing, single cell clustering, etc.
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include raw or normalized read count values for each sample

source_name: CD8-positive T cell precursors

growth_protocol: Mice were infected with LCMV-Arm intraperitoneally and enrichment of antigen-specific CD8 T cell precursors or LCMV-specific CD8 T cells at memory time points was performed.

extract_protocol:
RNA was isolated using Qiagen RNeasy plus mini kit (Bulk RNAseq) or cells were processed by FACS and supplied as input to the 10 Genomics single cell protocol (scRNAseq).
Sequencing libraries were created using TruSeq Stranded mRNA Library Prep Kit (Bulk RNA-seq) or the Chromium Single Cell 3’ Gene Expression Kit v2 (scRNA-seq) and were prepared for sequencing using standard Illumina protocols.
Bulk RNAseq and (single cell) scRNAseq

tag: tissue

value: spleen

tag: timepoint

value: 12 days post infection

tag: cell type (subset)

value: memory T cells


title: lymphocytic choriomeningitis virus infected naive T cells replicate 1

description:
LCMV.Naive.1
bulk mRNA
experiment_1_bulk_rnaseq_norm_counts.txt
experiment_1_bulk_rnaseq_raw_counts.txt

value: 90 days post infection

value: naive T cells


title: lymphocytic choriomeningitis virus infected naive T cells replicate 3

description:
LCMV.Naive.3
bulk mRNA
experiment_1_bulk_rnaseq_norm_counts.txt
experiment_1_bulk_rnaseq_raw_counts.txt


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D11_AD002_indexed

value: AI

value: male


title: lymphocytic choriomeningitis virus infected naive T cells replicate 2

description:
LCMV.Naive.2
bulk mRNA
experiment_1_bulk_rnaseq_norm_counts.txt
experiment_1_bulk_rnaseq_raw_counts.txt


title: murine cytomegalovirus infected long-lived effector cells replicate 2

description:
mCMV.LLEC.2
bulk mRNA
experiment_1_bulk_rnaseq_norm_counts.txt
experiment_1_bulk_rnaseq_raw_counts.txt

value: long-lived effector cells


title: murine cytomegalovirus infected long-lived effector cells replicate 1

description:
mCMV.LLEC.1
bulk mRNA
experiment_1_bulk_rnaseq_norm_counts.txt
experiment_1_bulk_rnaseq_raw_counts.txt


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D10_AD001_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_C9_AD010_indexed


title: murine cytomegalovirus infected long-lived effector cells replicate 3

description:
mCMV.LLEC.3
bulk mRNA
experiment_1_bulk_rnaseq_norm_counts.txt
experiment_1_bulk_rnaseq_raw_counts.txt


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_C9_AD008_indexed


title: lymphocytic choriomeningitis virus infected long-lived effector T cells replicate 2

description:
LCMV.LLEC.2
bulk mRNA
experiment_1_bulk_rnaseq_norm_counts.txt
experiment_1_bulk_rnaseq_raw_counts.txt

value: long-lived effector T cells


title: Male6_dmso_1_rep3

tag: cell type


title: memory P14 T cells 60 days post-lymphocytic choriomeningitis virus infection

description:
scRNAseq_Day60
single cell mRNA
experiment_2_singlecell_rnaseq_day12_day60_norm_counts.txt
experiment_2_singlecell_rnaseq_day12_day60_raw_counts.txt
experiment_2_singlecell_rnaseq_day60_norm_counts.txt
experiment_2_singlecell_rnaseq_day60_raw_counts.txt

value: 60 days post infection


title: lymphocytic choriomeningitis virus infected long-lived effector T cells replicate 3

description:
LCMV.LLEC.3
bulk mRNA
experiment_1_bulk_rnaseq_norm_counts.txt
experiment_1_bulk_rnaseq_raw_counts.txt


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_D10_AD002_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_C9_AD007_indexed


title: lymphocytic choriomeningitis virus infected long-lived effector T cells replicate 1

description:
LCMV.LLEC.1
bulk mRNA
experiment_1_bulk_rnaseq_norm_counts.txt
experiment_1_bulk_rnaseq_raw_counts.txt


title: lymphocytic choriomeningitis virus infected conventional effector memory T cells replicate 3

description:
LCMV.ConvMem.3
bulk mRNA
experiment_1_bulk_rnaseq_norm_counts.txt
experiment_1_bulk_rnaseq_raw_counts.txt

value: conventional effector memory T cells


title: lymphocytic choriomeningitis virus infected conventional effector memory T cells replicate 2

description:
LCMV.ConvMem.2
bulk mRNA
experiment_1_bulk_rnaseq_norm_counts.txt
experiment_1_bulk_rnaseq_raw_counts.txt


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_C8_AD012_indexed


title: 180612_CEMBA_mm_P56_P63_3D_CEMBA180412_3D_9_CEMBA180412_3D_10_C9_AD004_indexed