title: NPKR_3

type: genomic

first: Long-Jun

last: Ding

address: 18 Shuangqing Road, Haidian District

department: Research Center for Eco-Environmental Sciences

institute: Chinese Academy of Sciences

description: replicate 3

hyb_protocol: Then DNA was dried in a SpeedVac (ThermoSavant, Milford, MA, USA) at 45°C for 45 minutes. The hybridization was carried out at 42°C for 16 hours on a MAUI hybridization station (BioMicro, Salt Lake City, UT, USA).

scan_protocol: After purification, GeoChip microarrays were scanned by a scanner (MS 200 Microarray Scanner, NimbieGen) using a laser power and photomultiplier tube (PMT) gain of 100% and 75%, respectively.

data_processing: Signal intensities were quantified and processed using the data analysis pipeline as previously described (He et al., 2010). Then processed GeoChip data were analyzed using the following steps: (i) remove the poor quality spots, which were flagged as 1 or 3 by ImaGene or with a signal to noise ratio (SNR) of less than 2.0; (ii) the data were then logarithmically transformed and divided by the mean value of each slide.; (iii) transform the data to the natural logarithmic form; and (iv) remove genes detected in only one out of three samples from the same treatment.

organism: soil metagenome

source_name: Soil microbes under different fertilization treatments in a paddy soil

label_protocol: As previously described (Yang et al., 2013), DNA samples were labeled with the fluorescent dye Cy-5 using a random priming method and purified using the QIA quick purification kit (Qiagen, Valencia, CA, USA).

extract_protocol: Soil genomic DNA was extracted by a freeze-grinding, SDS-based method (Zhou et al., 1996). To purify it, prepare a 0.5 % low melting point agarose gel in 1X TAE. Mix fresh DNA with sufficient loading buffer (20X) so the buffer is 2X. Run the DNA sample slowly (50V, about 8 h) to get good resolution and prevent gel deformation. Excise bands, using a long wave length UV wand to visualize the band, and place the slice (about 1 ml) in a 15 ml purple capped conical tube. Melt the agarose gel at 65 °C until the gel slice has completely melted. Add an equal volume of 65 °C water and mix. Cool the gel at RT for 2 min, add an equal volume of cold, buffer-saturated phenol, invert several times to mix the sample, put on ice for 5 min, and centrifuge at 8000 rpm for 5 min at RT. Transfer the aqueous layer (top layer) to a fresh tube. Back extract the organic phase with 0.8 volume of fresh TE (10 mM Tris-HCl, pH 8.0, 1 mM EDTA) to 1 volume of phenol, mix put on ice for 5 min, and re-spin (8000 rpm, 5 min). Remove the new aqueous layer and combine with the first round aqueous layer. Add an equal volume of chloroform:isoamyl alcohol [24:1 (v/v)] to the twice extracted aqueous phase and rotate 5 min. Centrifuge at 8000 rpm for 5 min at RT. Transfer the aqueous (top) layer to a new tube. Add an equal volume of sec-butanol, mix 5 min on rotator and then centrifuge at 8000 rpm for 5 min at RT. Remove the butanol layer (upper layer) and discard. Repeat the butanol extraction at least 3 times. Continue the butanol extractions until the aqueous volume has been reduced to about 400-500 μl. Add 2.0 volume of 100% ice cold ethanol and 0.1 volume of 3 M NaOAc (pH 5.2) prior to overnight incubation at -20°C. DNA was precipitated by centrifugation for 30 minutes at 12000 rpm. Then supernatant was decanted and washed with 1 ml of 95% ethanol. DNA was air-dried and dissolved in 50 µl of nuclease-free water. DNA quality and quantity were measured using NanoDrop ND-1000 Spectrophotometer (NanoDrop Technologies Inc., Wilmington, DE, USA).

treatment_protocol: In September 2010, soil samples were collected from all of the treatments. Ten soil cores at a depth of 0-15 cm and diameter of 1.5 cm were randomly taken from each field plot and mixed thoroughly to generate a soil sample representing the plot. Soil samples were kept on ice when transporting to laboratory, and sieved with 2 mm mesh to remove roots and stones. Soil samples were stored at -80°C before DNA extraction.

tag: fertilization treatments

value: combined application of rice straw plus chemical fertilizers nitrogen, phosphorus and potassium


title: sample008_AH42_R1

status: Public on Sep 19 2020

type: SRA

city: Madrid

first: Alejandro

last: Higuera-Matas

state: Madrid

address: Calle Juan del Rosal 10

department: Psychobiology

country: Spain

institute: Universidad Nacional de Educación a Distancia (UNED)

library_source: transcriptomic

data_processing:
We used the Chipster analysis suite (https://chipster.csc.fi/) to perform data processing and analysis. Briefly, data quality analysis of the obtained raw data (singleEnd, stranded) was performed on FASTQC and PRINSEQ; no low-quality bases, or very few, were detected at the end of the reads.  Reads were aligned to reference genome Rattus_norvergicus. Rnor_6.0.87 using TOPHAPT and the alignment of the counts per read was performed using HTSeq.  Differential gene expression analysis was performed using CUFFDIFF with replicates analysis to explore the differences in transcriptomic profiles between factor levels.
Genome_build: Rnor_6.0.87
Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every sample

molecule: total RNA

organism: Rattus norvegicus

source_name: Male_THC_nucleus accumbens shell

extract_protocol:
Samples were homogenized, and RNA extracted according to the protocol, tools and reagents provided by RNeasy Mini Kit (Qiagen).
Libraries were prepared according to the instructions of the NEBNext Ultra Directional RNA Library Prep kit for Illumina kit (New England Biolabs), as detailed in “Chapter 1: Protocol for use with NEBNext Poly(A) mRNA Magnetic Isolation Module”. The input yield of total RNA to start the protocol was 1 µg quantified by an Agilent 2100 Bioanalyzer using an RNA 6000 nano LabChip kit. We performed the library amplification included in the cited protocol using a PCR of 14 cycles. The obtained libraries were validated and quantified by an Agilent 2100 Bioanalyzer using a DNA7500 LabChip kit and an equimolecular pool of libraries were titrated by quantitative PCR using the “Kapa-SYBR FAST qPCR kit forLightCycler480” (Kapa BioSystems) and a reference standard for quantification.

treatment_protocol: The adolescent chronic Δ9_THC treatment was administered every other day from PND 28 to PND 44. Rats received an intraperitoneal injection (2 mL/kg) of Δ9_THC at a dose of 3 mg/kg or vehicle (2 mL/kg). At PND 90, animals were deeply anaesthetised with isoflurane and sacrificed by decapitation. Brains were extracted and, with the help of a brain matrix, 1 mm thick coronal slices were obtained between 2.28 mm and 1.08 mm, approximately, anterior from bregma. With the help of two dissecting lancet-shaped needles, the NAcb (shell division)  was dissected according to the Paxinos and Watson atlas. The tissue samples were snap-frozen with dry ice and stored at -70º C.

tag: strain

value: Wistar

tag: Sex

value: Male

tag: treatment

value: THC

tag: tissue

value: Nucleus accumbens shell (bulk tissue)


title: P14W1_P3

city: Beijing

first: Shu

last: Zhang

state: Beijing

address: Peking University, No.5 Yiheyuan Road Haidian District

country: China

institute: BIOPIC

data_processing:
Illumina CASAVA version 1.8 were used to the basecalling.
Raw data sets were processed as previously described (Dong et al., 2018; Li et al., 2017; Zhong et al., 2018). Cells were split by the first 8-bp barcode in Reads2, and then the next 8-bp UMIs in Reads2 were added to the header in Reads1. After trimming the template switch oligo (TSO) and polyA sequences and removing the short reads (length<37 bp) and low-quality reads (N>10%) in Reads1, the clean reads were aligned by Tophat (version 2.0.12) to the hg19 genome downloaded from UCSC. Then, HTSeq was applied to count the uniquely mapped reads (Anders et al., 2015), and the number of different UMIs for each gene in each cell was considered the transcriptional count.
Genome_build: hg19
Supplementary_files_format_and_content: .csv files which include the count values of genes.

molecule: polyA RNA

organism: Homo sapiens

source_name: fetal pituitary cells

extract_protocol:
We used a mouth pipette to randomly pick single cells and used a modified STRT-seq protocol to construct an scRNA-seq library as previously described (Dong et al., 2018; Li et al., 2017; Zhong et al., 2018).
The cells were lysed in a lysis buffer with a 8-nt cell barcode and 8-nt UMI. Then, mRNA was reverse transcribed into cDNA with SuperScript™ II Reverse Transcriptase (18064014, Invitrogen). After preamplification, the samples with different cell barcodes were pooled together, and the mixture was labeled with a biotin modification by 4 cycles of PCR. Then, the full-length cDNA was sheared into fragments with approximately 300-bp lengths by Covaris (S2), and the 3’ cDNA was enriched by Dynabeads MyOne Streptavidin C1 (65002, Invitrogen) to construct a library according to the Kapa Hyper Prep Kit protocol (KK8505, Kapa Biosystems). Cleaned libraries were sequenced as paired-end 150-base reads on Illumina platform.

tag: cell type

value: fetal pituitary cells

tag: week post fertilization

tag: gender

value: female


title: L_NPKR_3

description: replicate 3

molecule: genomic DNA

value: combined application of reduced rice straw plus reduced chemical fertilizers nitrogen, phosphorus and potassium


title: sample007_AH53_R1


title: sample006_AH37_R1


title: NPKR_1

description: replicate 1


title: sample004_AH56_R1

source_name: Male_Vehicle_nucleus accumbens shell

value: Vehicle


title: p53 high [PATX 133]

city: Smithville

first: Bin

last: Liu

state: TX

address: 1808 Park Road 1C

department: Epigenetics and Molecular Carcinogenesis

country: USA

institute: University of Texas MD Anderson Cancer Center

description:
processed data file: PDX.rawreadcount.xlsx
MDA-PATX133_ATTACTCG_BC906JANXX

data_processing:
mapping with Tophat or Star
HT-seq count to count raw reads
DESeq2 for DE analysis
Genome_build: GRCm38/mm10
Supplementary_files_format_and_content: excel file

source_name: pancreatic cancer PDX_p53 high

extract_protocol: RNA purification- qiagen micro kit

tag: strain background

value: heterotopic pancreatic cancer tumors implanted in immunodeficient mice

tag: tissue type

value: pancreatic cancer PDX

tag: genotype/variation

value: p53 high


title: L_NPKR_1

description: replicate 1


title: NPR_3

description: replicate 3

value: combined application of rice straw plus chemical fertilizers nitrogen and phosphorus


title: p53 high [PATX 124]

description:
processed data file: PDX.rawreadcount.xlsx
MDA-PATX124_TCTCGCGC_AC8YYWANXX


title: p53 high [PATX 126]

description:
processed data file: PDX.rawreadcount.xlsx
PDX-PDAC-126-F3-R11_TCTCGCGC-AGGCGAAG_BC9WL7ANXX


title: sample003_AH32_R1


title: L_NPKR_2

description: replicate 2


title: NPKR_2

description: replicate 2


title: NR_3

description: replicate 3

value: combined application of rice straw plus chemical fertilizers nitrogen


title: p53 high [PATX 112]

description:
processed data file: PDX.rawreadcount.xlsx
MDA-PATX112_TCTCGCGC_AC8W8WANXX


title: NPR_2

description: replicate 2


title: NR_2

description: replicate 2


title: R_3

description: replicate 3

value: rice straw incorporation


title: NR_1

description: replicate 1


title: P15W1_G_B


title: P14W1_P1


title: p53 high [PATX 107]

description:
processed data file: PDX.rawreadcount.xlsx
MDA-PATX107_TCCGCGAA_AC8YYWANXX


title: P14W1_P2


title: sample002_AH48_R1


title: P10W2_G5


title: p53 high [PATX 102]

description:
processed data file: PDX.rawreadcount.xlsx
MDA-PATX102_ATTCAGAA_BC906JANXX


title: sample005_AH38_R1


title: P10W2_G6


title: R_2

description: replicate 2


title: NPR_1

description: replicate 1


title: sample001_AH25_R1


title: P10W2_G4


title: P10W2_G3


title: p53 high [PATX 100]

description:
processed data file: PDX.rawreadcount.xlsx
PDX-PDAC-100-F4-R11_TCCGCGAA-GTACTGAC_BC9WL7ANXX


title: p53 high [PATX 70]

description:
processed data file: PDX.rawreadcount.xlsx
PDX-PDAC-070-F3-R11_CGGCTATG-CAGGACGT_BC9W4UANXX


title: p53 high [PATX 81]

description:
processed data file: PDX.rawreadcount.xlsx
PDX-PDAC-081-F5-R11_TCCGCGAA-AGGCGAAG_BC9W4UANXX


title: P10W2_G2


title: Control_3

description: replicate 3

value: control without fertilizer


title: p53 high [PATX 55]

description:
processed data file: PDX.rawreadcount.xlsx
MDA-PATX55_ATTCAGAA_AC8YYWANXX


title: Control_2

description: replicate 2


title: P10W2_G1


title: p53 high [PATX 59]

description:
processed data file: PDX.rawreadcount.xlsx
MDA-PATX59_CGCTCATT_AC8YYWANXX


title: R_1

description: replicate 1


title: P10W1_M3


title: p53 high [PATX 50]

description:
processed data file: PDX.rawreadcount.xlsx
MDA-PATX50_TAATGCGC_BC906JANXX


title: p53 high [PATX 46]

description:
processed data file: PDX.rawreadcount.xlsx
PDX-PDAC-046-F4-R11_CGGCTATG-CCTATCCT_BC9W4UANXX


title: P09W1_G4

value: male


title: P10W1_M1


title: n2_LK56_B

type: RNA

city: Houston

first: Natalia

middle: V

last: Kirienko

address: 6100 Main St. MS140

department: BioSciences

institute: Rice University

hyb_protocol: Affymetrix standard protocol

scan_protocol: Affymetrix standard protocol

data_processing: Data were analyzed with GC-RMA in the R programming language, using the Bioconductor package gcrma. Analysis was performed using default settings.

label: biotin

organism: Caenorhabditis elegans

source_name: N2, S Basal (liquid), LK56, 8h

label_protocol: Affymetrix standard protocol

growth_protocol: N2 worms were synchronized by hypochlorite treatment. Arrested L1s were plated on 10 cm NGM plates seeded with E. coli OP50 and grown 56 h at  22°C until they reached young adult stage.

extract_protocol: RNA was extracted using TRI Reagent (Molecular Research Center) according to manufacturer's instructions and purified using an RNeasy column (Qiagen).

treatment_protocol: Animals were grown until young adult stage. Then they were washed off the plates into 15 mL conicals, rinsed 2 times in S Basal, supplemented with E. coli OP50 for foos, and dispensed into 6-well plates, 6,000 worms/well. LK molecules at 100 uM final or corresponding amount of DMSO solvent control were added to wells.

tag: media

value: S Basal

value: Whole organism


title: n2_LK38_C

source_name: N2, S Basal (liquid), LK38, 8h


title: n2_LK56_C


title: P09W1_G3


title: Control_1

description: replicate 1


title: S3_rep3

city: Lund

first: Robert

last: Carlsson

address: Sölvegatan 17

department: IKVL

country: Sweden

institute: Lund University

description: RMA normalized

hyb_protocol: Samples were hybridized with GeneChip Human Exon 1.0 ST Arrays (Affymetrix) at the SciBlue facility at Lund University.

scan_protocol: Array scanning was performed according to the manufacturer's instruction (Affymetrix)

data_processing: Data was RMA normalized and analysed in R3.6.3

label: Biotin Allonamide Triphosphate

source_name: FACS sorted cells

label_protocol: Labelling reaction at 37 degrees Biotin Allonamide Triphosphate 996pg

growth_protocol: FACS sorted cells from blood samples of patients

extract_protocol: Total RNA was extracted from human monocytes and G-MDSCs from healthy donors, and from sepsis and breast cancer patients using Trizol. The RNA samples were then treated with DNAse I. RNA integrity was evaluated using Bioanalyzer Pica-kit

treatment_protocol: no treatment

tag: origin cells

value: Cells of blood from sepsis patient

value: unknown


title: BC_rep3

description: RMA normalized

value: Cells of blood from breast cancer


title: BC_rep1

description: RMA normalized


title: S2_rep2

description: RMA normalized


title: BC_rep2

description: RMA normalized


title: n2_LK56_A


title: S1_rep1

description: RMA normalized


title: HCMo_rep2

description: RMA normalized

value: Control blood cells


title: n2_LK35_C

source_name: N2, S Basal (liquid), LK35, 8h


title: p53 high [PATX 43]

description:
processed data file: PDX.rawreadcount.xlsx
PDX-PDAC-043-F5-R11_CGGCTATG-ATAGAGGC_BC9W4UANXX


title: n2_LK38_B


title: n2_LK38_A


title: P10W1_M2

value: fetal pituitary cells

value: female


title: P09W1_G2


title: HCMo_rep1

description: RMA normalized


title: n2_LK35_B


title: P08W2_P5


title: n2_LK35_A


title: n2_LK34_C

source_name: N2, S Basal (liquid), LK34, 8h


title: HCN_rep3

description: RMA normalized


title: HCMo_rep3

description: RMA normalized


title: p53 high [PATX 45]

description:
processed data file: PDX.rawreadcount.xlsx
MDA-PATX45_CGGCTATG_AC8YYWANXX


title: P08W2_P3


title: Control non-swarming mosquito Replicate 2

city: Shanghai

first: Sibao

last: Wang

address: 300 Feng Lin Road

department: CAS Center for Excellence in Molecular Plant Science/ Institute of Plant Physiology and Ecology

description: Biological replicate 2 of 3,non-swarming mosquito

hyb_protocol: Probe hybridization to the microarray slides was performed with 2 μg cRNA probes.Hybridization buffer to stop fragmentation. Samples were applied to microarrays enclosed in A4 mixer chambers.After hybridization, slides were washed sequential

scan_protocol: Microarray slides were scanned using an Axon GenePix 4200AL scanner at 10-μm pixel size (Axon Instruments, Union City, California, USA) The scanned images were analyzed using feature extraction software (Agilent)

data_processing: gProcessedSignal and rProcessedSignal from Agilent feature extraction output files were imported into Partek and quantile normalized and log2 transformed.

organism: Anopheles gambiae

source_name: laboratory mosquito

label_protocol: Cy5-labeled control and Cy3-labeled experimental cRNA probes were generated from 200 ng of RNA using Agilent Technologies Low Input Quick Amp Labeling Kit according to the manufacturer's instructions.

extract_protocol:
Mosquitoes, mostly males, were collected in swarms using sweeping net during dusk. The indoor resting males with antennal fibrillae becoming erect were collected in inhabited houses using vacuum aspiration just prior to swarming time.
Total RNA extracted using Trizol following manufacturer's instructions, and CTAB extraction to Remove Melanin

value: head

organism: Anopheles coluzzii

source_name: field mosquito

tag: group

value: non-swarming


title: P08W2_P1


title: P08W2_P4


title: KPwmC tumor 4 [WM6902]

description:
processed data file: raw.count.xls
WM_6902_tumor_CGATGT

organism: Mus musculus

source_name: pancreatic tumor_KPwmC

value: moribund tumor

value: pancreatic tumor

value: KPwmC


title: P08W1_2


title: n2_LK34_B


title: p53 high [PATX 32]

description:
processed data file: PDX.rawreadcount.xlsx
PDX-PDAC-032-F4-R11_TAATGCGC-GTACTGAC_BC9W4UANXX

value: heterotopic pancreatic cancer tumors implanted in immunodeficient mice

value: pancreatic cancer PDX

value: p53 high


title: swarming mosquito Replicate 3

description: Biological replicate 3 of 3,swarming mosquito

value: swarming


title: Control non-swarming mosquito Replicate 3

description: Biological replicate 3 of 3,non-swarming mosquito


title: HCN_rep2

description: RMA normalized


title: KPwmC tumor 3 [WM6804]

description:
processed data file: raw.count.xls
WM_6804_tumor_TTAGGC


title: HCN_rep1

description: RMA normalized


title: n2_LK32_C

source_name: N2, S Basal (liquid), LK32, 8h

value: S Basal


title: P08W2_P2


title: P08W1_1

value: male


title: Control non-swarming mosquito Replicate 1

description: Biological replicate 1 of 3,non-swarming mosquito


title: KPflC tumor 4 [FL7952]

description:
processed data file: raw.count.xls
FL_7952_tumor_CAGATC

source_name: pancreatic tumor_KPflC

value: KPflC


title: swarming mosquito Replicate 2

description: Biological replicate 2 of 3,swarming mosquito


title: KPflC tumor 3 [FL7782]

description:
processed data file: raw.count.xls
FL_7782_tumor_ACTTGA


title: n2_LK32_B

value: Whole organism


title: KPwmC tumor 1 [WM6844]

description:
processed data file: raw.count.xls
6844_tumor_ATCACG


title: KPflC tumor 2 [FL8488]

description:
processed data file: raw.count.xls
FL_8488_tumor_GCCAAT


title: KPwmC tumor 2 [WM7021]

description:
processed data file: raw.count.xls
WM_7021_tumor_TGACCA


title: P07W1_2


title: swarming mosquito Replicate 1

description: Biological replicate 1 of 3,swarming mosquito


title: n2_LK34_A


title: mRIf1 siRNA 72 h

city: Setagaya

first: Naoko

last: Yoshizawa-Sugata

state: Tokyo

address: 2-1-6 Kami-kitazawa

department: Research Center for Genome & Medical Sciences

country: Japan

institute: Tokyo Metropolitan Institute of Medical Science

description: Gene expression in mRif1 siRNA-transfected E14tg2a cells, 72 h

hyb_protocol: 1.65µg of the labelled samples were hybridized to the Mouse Gene Expression v2 Microarray Slides for 17 hours at 65ºC at 10 rpm in a rotating hybridization oven.

scan_protocol: The slides were scanned  immediately after washing on SureScan Microarray Scanner (Agilent Technologies) using one color scan setting for 4x44k array slides (Scan resolution 5 um, Dye channel is set to Green and Green PMT is set to 100%).

data_processing: Data was obtained using the Agilent Feature Extraction software (v11.5.1.1) with set to default for all parameters and analyzed with GeneSpring GX software.

source_name: E14, 72hr, mRif1 KD

label_protocol: Cy3-labeled cRNA was prepared from 0.2µg RNA using a Low Input Quick Amp Labeling Kit.

growth_protocol: Cells were maintained in the ES medium consisting of DMEM supplemented with 15% fetal bovine serum (Gibco), 4 mM L-glutamine, 0.1 mM 2-mercaptoethanol, 0.1 mM non-essential amino acids, 1 mM sodium pyruvate, and mouse leukemia inhibitory factor (LIF; ESGRO, Millipore; 1 x 103 U/ mL).

extract_protocol: RNA was prepared using RNeasy micro kit (QIAGEN) and  re-purified.

treatment_protocol: Cells were transfected with control siRNA, mRif1 sRNA or untreated, for 48 or 72 h.

value: E14tg2a cells


title: P07W1_1


title: environmental_sample_pool_A

status: Public on Sep 18 2020

city: New York

first: Leopoldo

middle: Nicolas

last: Segal

state: NY

address: 462 First Avenue NBV 6N5-6N8

department: Medicine

institute: NYU School of Medicine

description:
ribosomal DNA
The SRR for this sample (SRR4298944) contains data for the forward and reverse strand as well as the barcode information in fastq format. The three separate fastq files can be obtained with the command: fastq-dump --split-files SRR4298944.

library_source: genomic

data_processing:
Library strategy: DNA-seq
demultiplexing and filtering of short (<150 nt) and low quality reads. Barcode information is available in the file "Sample_barcode_metadata.txt" on the series record.
de novo clustering of the sequences into operational taxonomic units (OTUs) with the UCLUST program using a 97% similarity threshold
taxonomical assignment of each OTU by running the RDP Classifier(8) at 80% bootstrap confidence on a selected representative sequence from each OTU;
alignment of representative sequences using PyNAST(9) with the Greengenes core-set alignment template
phylogenetic tree reconstruction from the representative sequences for each OTUs using the FASSTTREE program
Bray Curtis distance calculations
Supplementary_files_format_and_content: read abundance for OTUs. The file Sample_barcode_metadata.txt.gz on the series record contains the barcode information and sample source for all raw data contained in the multiplexed raw data file.

organism: uncultured bacterium

source_name: environmental samples that include metal working fluid and air from a machine facility

extract_protocol:
ion exchange column (Qiagen)
Amplicon library preparation was performed using an automated platform (Biomek 4000) using a custom liquid handling method. For each sample, the V4 region of the bacterial 16S rRNA gene was amplified in duplicate reactions, using primer set 515F/806R, which nearly universally amplifies bacterial and archaeal 16S rRNA genes.1,2 Each unique barcoded amplicon was generated in pairs of 25μl reactions with the following reaction conditions: 11μl PCR-grade H2O, 10μl Hot MasterMix (5 Prime Cat# 2200410), 2μl of forward and reversed barcoded primer (5μM) and 2μl template DNA. Reactions were run on a C1000 Touch Thermal Cycler (Bio-Rad) with the following cycling conditions: initial denaturing at 94°C for 3 min followed by 35 cycles of denaturation at 94°C for 45 seconds, annealing at 58°C for 1 minute, and extension at 72 C for 90 seconds, with a final extension of 10 min at 72°C. Amplicons were quantified using Agilent 2200 TapeStation system and pooled. Purification was then performed using Ampure XT (Beckman Coulter Cat# A63882) as per the manufacturer instructions

tag: linkerprimersequence

value: CCGGACTACHVGGGTWTCTAAT


title: mRIf1 siRNA 48 h

description: Gene expression in mRif1 siRNA-transfected E14tg2a cells, 48 h

source_name: E14, 48hr, mRif1 KD


title: n2_DMSO_C

source_name: N2, S Basal (liquid), DMSO, 8h

value: DMSO


title: environmental_sample_pool_B

description:
ribosomal DNA
The SRR for this sample (SRR4298946) contains data for the forward and reverse strand as well as the barcode information in fastq format. The three separate fastq files can be obtained with the command: fastq-dump --split-files SRR4298946.

source_name: environmental samples that include metal working fluid and air from a machine facility and lung tissue samples


title: KPflC tumor 1 [FL7699]

description:
processed data file: raw.count.xls
FL_7699_tumor_ACAGTG


title: n2_LK32_A


title: n2_DMSO_A


title: southern1_24_a

city: Leuven

first: Janne

last: Swaegers

address: Charles Deberiotstraat 32

country: Belgium

institute: KU Leuven

data_processing:
Raw reads were visually inspected with FastQC version 0.11.5
Trimming using Trimmomatic version 0.36 for adapter sequences
The trimmed reads were de novo assembled with Trinity version 2.2.0 using default parameters (kmer length = 25)
Duplicate transcripts were removed by clustering the assembly using CD-HIT-EST version 4.6.6 at 95% sequence similarity
Repeatmasker version 4.0.6 was used to remove transcripts containing ribosomal RNA
Only transcripts with at least one read per million in at least two samples were kept
Read abundance was estimated using featureCounts in the Subread package version 1.5.1
Genome_build: de novo, assembly.fasta
Supplementary_files_format_and_content: counts.txt is a count matrix with every column containing the counts of a sample for every transcript

organism: Ischnura elegans

source_name: whole body F0 larva

growth_protocol: Larvae were fed twice per day with Artemia nauplii ad libitum for six days per week. Once the larvae moulted into the final instar, they were fed daily

extract_protocol:
RNA was extracted from six larvae per combination of temperature and population using Qiagen’s RNeasy Plus Universal Mini Kit and standardized instructions of the manufacturer.
The pooled RNA samples were sent to BGI, Hong Kong where 160 bp libraries were constructed using the TruSeq technology. Next, the libraries were 101 bp Paired-End sequenced on a Hiseq4000 platform

treatment_protocol: Per population, larvae from 10 females (total of 60 females, 1756 larvae) were used for the rearing experiment. For the current study we used a subset of 72 male larvae (from 8-9 mothers per population). After hatching larvae were randomly divided over two temperature treatments, 20°C and 24°C and reared individually at this temperature up to the final larval stage in the laboratory

value: whole body

value: 24 degrees C

tag: population


title: southern1_24_b


title: southern2_24_b


title: southern3_20_a

value: 20 degrees C


title: northern3_24_b


title: southern3_24_a


title: southern2_24_a


title: southern2_20_a


title: southern3_20_b


title: southern1_20_b


title: southern1_20_a


title: southern3_24_b


title: southern2_20_b


title: northern3_24_a


title: n2_DMSO_B


title: control siRNA 72 h

description: Gene expression in control siRNA-transfected E14tg2a cells, 72 h

source_name: E14, 72hr, control


title: northern2_24_b


title: Non-treated

description: Gene expression in non-treated E14tg2a cells

source_name: E14, 0hr, non-treated


title: northern2_20_a


title: northern1_24_b


title: northern2_24_a


title: northern2_20_b


title: northern3_20_a


title: scRNA-Seq of fresh mouse gbm cells

city: Zhejiang

first: Chong

address: NO.866 of Yuhang Tong Road

institute: Medical College of Zhejiang University

data_processing:
Sequenced reads were aligned to reference genome by cellranger.
The matrix was separated according to the cell type judgment result obtained by the cellranger.
Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using a protocol from Chepelev et al., Nucleic Acids Research, 2009. In short, exons from all isoforms of a gene were merged to create one meta-transcript. The number of reads falling in the exons of this meta-transcript were counted and normalized by the size of the meta-transcript and by the size of the library.
To select high quality cells for further analysis: 1) Removed genes detected in < 3 cells. 2) Filted cells with unique molecular identified (UMI) ≤ 150 or ≥ 4500 (UMI ≤ 200 or ≥ 6,500 were removed in mouse cell line sample; UMI ≤ 200 or ≥ 7,000 were removed in human cell line sample). 3) Removed cells that have ≥ 20% mitochondrial counts (cells that have ≥ 15% mitochondrial counts were filtered in cell line sample). Normalization was performed using the function “LogNormalize” of the R package Seurat.
Genome_build: mm10, hg19, GRCh38

source_name: Brain

growth_protocol: The mouse and human GBM cell lines were maintained in human GBM complete media, which contained Neurobasal (Gibco, #21103-049); Glutamine (Gibco, #21051-024), 1x; Pen/Strep (HyClone, #SV30010), 1x; Na Pyruvate (Gibco, #11360-070), 1x; Gentamycin (Sigma, #46305), 50 µg/mL; Trace Elements B (Cellgro, #99-175-CI), 1x; d-Biotin (Sigma, #B4639), 10 ng/mL; SATO 10 µl/mL; Transferrin (Sigma, #T-1147), 100 µg/mL; BSA (Sigma, #A-4161), 100 µg/mL; Putrescine (Sigma, #P-5780), 16 µg/mL; Progesterone (Sigma, #P8783), 60 ng/mL; Sodium selenite (Sigma, #S5261), 40 ng/mL; Insulin (Sigma, 3I6634), 5 µl/mL; B-27(without Vitamin) (Gibco, #12587-010), 20 µl/mL supplemented with growth factors as indicated. The concentration of growth factors was used as following: EGF (50 ng/ml) /FGF (20 ng/ml) or PDGFAA (20 ng/ml). 1/2 media with fresh growth factors were regularly replaced every 4-5 days.

extract_protocol:
Primary tumor tissue or cell line were resuspended to single cell suspension for further experiment.
scRNA libraries were prepared for sequencing using standard Illumina protocols

value: brain tumor

tag: genotype


title: northern1_24_a


title: northern1_20_b


title: Th1 CAR-T cell rep1

first: Lintao

state: Texas

address: 6550 FANNIN ST, RI-SMITH8-053

department: Cancer

institute: Houston Methodist Research Insititute

description:
Th1_1
Th1_1.gene.fpkm.xls

data_processing:
The reads mapped to rRNA were removed.
The sequencing reads which containing low-quality, adaptor-polluted and high content of unknown base reads were processed to be removed.
Fragements Per Kilobase of exon per Megabase of library size (FPKM) were calculated using a protocol from Chepelev et al., Nucleic Acids Research, 2009. In short, exons from all isoforms of a gene were merged to create one meta-transcript. The number of reads falling in the exons of this meta-transcript were counted and normalized by the size of the meta-transcript and by the size of the library.
Clean reads were mapped to reference genome using HISAT2.
Genome_build: hg19
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample …

source_name: Human T cell

growth_protocol: T cells were cultured in T Cell Expansion media (ThermoFisher) supplemented with 100 U/mL penicillin, 100 mg/mL streptomycin sulfate and 2 mM GlutaMAX, and stimulated with Human T-Activator CD3/CD28 Dynabeads (ThermoFisher) at a ratio of 3:1 (beads to cells). Twenty-four hours after activation, T cells were transduced with lentivirus. Th1 CAR-T cells were cultured under Th1 condition ( 100 IU/mL of  IL2),  Th9 CAR-T cells were cultured under Th9 condition  ( 10 ng/ml of IL4 ,  1 ng/ml of TGF-β, 10 μg/ml of anti-IFN-γ antibody, and 100 IU/mL of  IL2)

extract_protocol:
RNeasy Mini Kit (Qiagen)
RNA libraries were prepared for sequencing using standard Illumina protocols

value: Human T cell


title: Th9 CAR-T cell rep1

description:
Th9_1
Th9_1.gene.fpkm.xls


title: northern3_20_b


title: Th1 CAR-T cell rep3

description:
Th1_3
Th1_3.gene.fpkm.xls


title: Th9 CAR-T cell rep3

description:
Th9_3
Th9_3.gene.fpkm.xls


title: Th9 CAR-T cell rep2

description:
Th9_2
Th9_2.gene.fpkm.xls


title: Th1 CAR-T cell rep2

description:
Th1_2
Th1_2.gene.fpkm.xls


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H9_ad008

city: La Jolla

first: Joseph

middle: R

last: Ecker

state: CA

address: 10010 North Torrey Pines Road

department: Genomic Analysis Laboratory

institute: HHMI-Salk-Institute

data_processing:
Library strategy: snmC-seq2
Reads trimed with Cutadapt for adapters and low quality bases (Phred score >= 20)
The resulting files are mapped to in-silico bisulfite converted mm10 reference genome using Bismark.
The raw BAM file will be filtered using samtools to filter reads by MAPQ > 10 and use Picard to remove PCR duplicates
Tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls will be generated with in-house developing package using the filtered BAM files
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls. Columns are: 1. Chromosome, 2. Position, 3. Strand, 4. Cytosin Context, 5. methylated base call, 6. total mapped base call, 7. not used, place holder column

source_name: C57BL/6J Male P56 Mouse Brain

growth_protocol: All mice are C57BL/6J Male, brain dissected in P56

extract_protocol:
the brain of P56 male C57BL/6J mouse will be removed and cut into 600 microns thick coronal sections using a tissue matrix. Then each region will be microdissected from the coronal tissue sections based on identified landmarks and brain atlas coordinates, as defined in Allen brain atlas CCF3 (Avaliable at: http://atlas.brain-map.org). For each dissection region, the tissue from several littermate mice will be combined together into to separate replicate groups. The number of animals in a replicate depends on the size of the tissue. Nuclei preparation will be stained with the fluorescently labeled anti-NeuN body and nucleic acid stain Hoechst 33342.
Library prepared following protocol discribed in Luo, Chongyuan, Angeline Rivkin, Jingtian Zhou, Justin P. Sandoval, Laurie Kurihara, Jacinta Lucero, Rosa Castanon, et al. 2018. “Robust Single-Cell DNA Methylome Profiling with snmC-seq2.” Nature Communications 9 (1): 3824.

tag: brain region

value: CA

tag: age


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H9_ad002

tag: Sex

tag: strain


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H9_ad010


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H8_ad006


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H9_ad012


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H9_ad007


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H9_ad004


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H8_ad001


title: northern1_20_a

tag: tissue

tag: treatment


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H9_ad006


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H8_ad012


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H8_ad010


title: P09W1_G1

source_name: fetal pituitary cells

tag: cell type

value: fetal pituitary cells

tag: week post fertilization


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H7_ad010


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H8_ad004


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H7_ad012


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H8_ad002


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H8_ad008


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H7_ad006


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H6_ad001


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H7_ad002


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H6_ad012


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H6_ad010


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H7_ad008


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H7_ad001


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H6_ad006


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H5_ad012


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H6_ad002


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H6_ad007


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H5_ad010


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H4_ad004


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H4_ad006


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H6_ad004


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H6_ad008


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H5_ad004


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H5_ad002


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H7_ad007


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H4_ad012


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H5_ad001


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H5_ad008


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H4_ad008


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H3_ad004


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H4_ad002


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H3_ad010


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H3_ad008


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H3_ad006


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H7_ad004


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H2_ad012


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H5_ad007


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H12_ad010


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H2_ad006


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H2_ad008


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H2_ad001


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H2_ad004


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H12_ad006


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H2_ad002


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H11_ad008


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H11_ad006


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H11_ad004


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H11_ad001


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H12_ad012


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H11_ad007


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H10_ad010


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H10_ad008


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H12_ad002


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H12_ad008


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H12_ad004


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H11_ad012


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H11_ad010


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H3_ad002


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H12_ad007


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H10_ad004


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H10_ad012


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H10_ad006


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H10_ad007


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H1_ad006


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G9_ad002


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G9_ad006


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G9_ad004


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H10_ad001


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G8_ad007


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G9_ad012


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G8_ad012


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G8_ad004


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H1_ad001


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H1_ad007


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H1_ad004


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H1_ad008


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H1_ad002


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H11_ad002


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H1_ad010


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G8_ad010


title: CEMBA190711_8E_3_CEMBA190711_8E_4_H1_ad012


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G8_ad001


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G9_ad008


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G7_ad001


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G9_ad010


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G9_ad001


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G8_ad008


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G7_ad004


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G7_ad006


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G7_ad012


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G8_ad002


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G7_ad002


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G7_ad007


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G8_ad006


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G6_ad006


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G6_ad002


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G6_ad004


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G6_ad012


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G6_ad007


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G5_ad010


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G5_ad012


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G6_ad008


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G5_ad007


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G5_ad001


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G4_ad008


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G5_ad002


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G4_ad006


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G5_ad008


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G6_ad010


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G3_ad010


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G5_ad004


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G7_ad010


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G3_ad001


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G3_ad006


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G3_ad007


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G2_ad012


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G4_ad002


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G3_ad008


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G2_ad001


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G3_ad002


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G3_ad012


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G2_ad010


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G2_ad007


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G7_ad008


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G3_ad004


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G2_ad004


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G2_ad002


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G12_ad012


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G2_ad008


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G12_ad008


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G12_ad007


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G2_ad006


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G12_ad002


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G12_ad004


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G11_ad002


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G10_ad010


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G11_ad001


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G11_ad008


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G11_ad012


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G12_ad001


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G11_ad006


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G1_ad006


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G10_ad001


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G10_ad002


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G10_ad007


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G1_ad002


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G11_ad004


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G1_ad012


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G10_ad006


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G10_ad004


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G1_ad007


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G11_ad007


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G1_ad008


title: CEMBA190711_8E_3_CEMBA190711_8E_4_F9_ad012


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G11_ad010


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G1_ad004


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G1_ad001


title: CEMBA190711_8E_3_CEMBA190711_8E_4_F9_ad010


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G10_ad008


title: CEMBA190711_8E_3_CEMBA190711_8E_4_G12_ad006


title: CEMBA190711_8E_3_CEMBA190711_8E_4_F9_ad001


title: CEMBA190711_8E_3_CEMBA190711_8E_4_F9_ad007


title: CEMBA190711_8E_3_CEMBA190711_8E_4_F9_ad004


title: CEMBA190711_8E_3_CEMBA190711_8E_4_F9_ad008


title: CEMBA190711_8E_3_CEMBA190711_8E_4_F8_ad010


title: CEMBA190711_8E_3_CEMBA190711_8E_4_F8_ad006


title: CEMBA190711_8E_3_CEMBA190711_8E_4_F8_ad002


title: CEMBA190711_8E_3_CEMBA190711_8E_4_F8_ad008


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title: CEMBA190711_8E_1_CEMBA190711_8E_2_C3_ad012


title: CEMBA190711_8E_1_CEMBA190711_8E_2_C12_ad004


title: CEMBA190711_8E_1_CEMBA190711_8E_2_C12_ad001


title: CEMBA190711_8E_1_CEMBA190711_8E_2_C4_ad004


title: CEMBA190711_8E_1_CEMBA190711_8E_2_C11_ad012


title: CEMBA190711_8E_1_CEMBA190711_8E_2_C11_ad006


title: CEMBA190711_8E_1_CEMBA190711_8E_2_C11_ad007


title: CEMBA190711_8E_1_CEMBA190711_8E_2_C11_ad004


title: CEMBA190711_8E_1_CEMBA190711_8E_2_C11_ad002


title: CEMBA190711_8E_1_CEMBA190711_8E_2_C10_ad012


title: CEMBA190711_8E_1_CEMBA190711_8E_2_C11_ad008


title: CEMBA190711_8E_1_CEMBA190711_8E_2_C10_ad010


title: CEMBA190711_8E_1_CEMBA190711_8E_2_C10_ad008


title: CEMBA190711_8E_1_CEMBA190711_8E_2_C10_ad001


title: CEMBA190711_8E_1_CEMBA190711_8E_2_C10_ad002


title: CEMBA190711_8E_1_CEMBA190711_8E_2_C10_ad007


title: CEMBA190711_8E_1_CEMBA190711_8E_2_C10_ad006


title: CEMBA190711_8E_1_CEMBA190711_8E_2_C1_ad001


title: CEMBA190711_8E_1_CEMBA190711_8E_2_C1_ad007


title: CEMBA190711_8E_1_CEMBA190711_8E_2_C1_ad006


title: CEMBA190711_8E_1_CEMBA190711_8E_2_C1_ad002


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B9_ad001


title: CEMBA190711_8E_1_CEMBA190711_8E_2_C1_ad004


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B9_ad012


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B9_ad007


title: CEMBA190711_8E_1_CEMBA190711_8E_2_C1_ad010


title: CEMBA190711_8E_1_CEMBA190711_8E_2_C11_ad010


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B9_ad004


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B9_ad006


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B8_ad012


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B9_ad010


title: CEMBA190711_8E_1_CEMBA190711_8E_2_C1_ad008


title: CEMBA190711_8E_1_CEMBA190711_8E_2_C1_ad012


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B8_ad007


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B8_ad001


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B8_ad006


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B7_ad001


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B8_ad004


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B8_ad002


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B9_ad008


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B7_ad012


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B8_ad008


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B7_ad004


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B8_ad010


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B7_ad010


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B7_ad007


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B7_ad006


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B6_ad001


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B6_ad007


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B6_ad010


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B6_ad006


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B6_ad008


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B6_ad002


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B6_ad004


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B5_ad012


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B5_ad010


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B5_ad007


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B5_ad001


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B5_ad004


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B5_ad008


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B5_ad006


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B5_ad002


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B4_ad006


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B4_ad012


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B4_ad001


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B6_ad012


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B4_ad002


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B4_ad008


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B4_ad004


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B3_ad007


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B2_ad007


title: CEMBA190711_8E_1_CEMBA190711_8E_2_B2_ad010