title: CEMBA190117_6A_3_CEMBA190117_6A_4_G6_ad002


title: CEMBA190117_6A_3_CEMBA190117_6A_4_G5_ad007

status: Public on Sep 29 2020

type: SRA

city: La Jolla

first: Joseph

middle: R

last: Ecker

state: CA

address: 10010 North Torrey Pines Road

department: Genomic Analysis Laboratory

country: USA

institute: HHMI-Salk-Institute

library_source: genomic

data_processing:
Library strategy: snmC-seq2
Reads trimed with Cutadapt for adapters and low quality bases (Phred score >= 20)
The resulting files are mapped to in-silico bisulfite converted mm10 reference genome using Bismark.
The raw BAM file will be filtered using samtools to filter reads by MAPQ > 10 and use Picard to remove PCR duplicates
Tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls will be generated with in-house developing package using the filtered BAM files
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls. Columns are: 1. Chromosome, 2. Position, 3. Strand, 4. Cytosin Context, 5. methylated base call, 6. total mapped base call, 7. not used, place holder column

molecule: genomic DNA

organism: Mus musculus

source_name: C57BL/6J Male P56 Mouse Brain

growth_protocol: All mice are C57BL/6J Male, brain dissected in P56

extract_protocol:
the brain of P56 male C57BL/6J mouse will be removed and cut into 600 microns thick coronal sections using a tissue matrix. Then each region will be microdissected from the coronal tissue sections based on identified landmarks and brain atlas coordinates, as defined in Allen brain atlas CCF3 (Avaliable at: http://atlas.brain-map.org). For each dissection region, the tissue from several littermate mice will be combined together into to separate replicate groups. The number of animals in a replicate depends on the size of the tissue. Nuclei preparation will be stained with the fluorescently labeled anti-NeuN body and nucleic acid stain Hoechst 33342.
Library prepared following protocol discribed in Luo, Chongyuan, Angeline Rivkin, Jingtian Zhou, Justin P. Sandoval, Laurie Kurihara, Jacinta Lucero, Rosa Castanon, et al. 2018. “Robust Single-Cell DNA Methylome Profiling with snmC-seq2.” Nature Communications 9 (1): 3824.

tag: brain region

value: ACA,PL,ILA

tag: Sex

value: male

tag: age

tag: strain


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title: CEMBA190117_6A_3_CEMBA190117_6A_4_A8_ad010


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A8_ad006


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A7_ad012


title: CEMBA190117_6A_3_CEMBA190117_6A_4_B10_ad002


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A8_ad004


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A7_ad010


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A7_ad007


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A7_ad006


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A7_ad002


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A7_ad001


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A7_ad008


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A6_ad001


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A6_ad010


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A6_ad006


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A8_ad008


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A6_ad004


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A6_ad007


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A6_ad012


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A8_ad002


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A6_ad008


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A5_ad007


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A4_ad012


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A5_ad012


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A4_ad001


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A5_ad010


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A4_ad007


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A9_ad008


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A4_ad006


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A4_ad004


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A4_ad002


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A4_ad008


title: CEMBA190117_6A_3_CEMBA190117_6A_4_B1_ad010


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A3_ad012


title: CEMBA190117_6A_3_CEMBA190117_6A_4_B1_ad007


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A3_ad001


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A3_ad007


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A3_ad006


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A3_ad004


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A3_ad010


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A6_ad002


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A2_ad012


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A3_ad008


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A5_ad001


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A2_ad007


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A2_ad001


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A2_ad006


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A2_ad008


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A12_ad012


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A2_ad002


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A2_ad004


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A12_ad008


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A12_ad010


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A12_ad006


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A11_ad007


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A11_ad006


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A11_ad008


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A11_ad004


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A3_ad002


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A11_ad002


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A11_ad010


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A10_ad007


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A10_ad006


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A10_ad012


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A11_ad001


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A10_ad002


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A10_ad008


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A10_ad001


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A1_ad010


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A12_ad007


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A1_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H9_ad012


title: CEMBA190117_6A_3_CEMBA190117_6A_4_B6_ad012


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A1_ad006


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A1_ad012


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A1_ad004


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A1_ad002


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A11_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H9_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H9_ad007


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A10_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H9_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H9_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H8_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H9_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H9_ad002


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A10_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H9_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H8_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H8_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H8_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H8_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H8_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H8_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H7_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H7_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H7_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H5_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H6_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H6_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H7_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H7_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H5_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H5_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H8_ad002


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A12_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H5_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H5_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H5_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H5_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H7_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H4_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H4_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H4_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H4_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H4_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H4_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H3_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H7_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H3_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H3_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H3_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H2_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H2_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H2_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H2_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H5_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H2_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H12_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H4_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H12_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H12_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H12_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H4_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H12_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H12_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H11_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H11_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H11_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H2_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H2_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H11_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H10_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H11_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H11_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H11_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H11_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H10_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H10_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H10_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H1_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H1_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H3_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H12_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H1_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H10_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H12_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H1_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H1_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H1_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H1_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H1_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G9_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G9_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G9_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G9_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G8_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G8_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G8_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G8_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G8_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G8_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G8_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_H10_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G7_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G7_ad001


title: MeOH L3 brain

city: LEUVEN

first: Stein

last: Aerts

address: Herestraat 49, bus 602

department: Center for Brain & Disease Research

country: Belgium

institute: KULeuven/VIB

library_source: transcriptomic

data_processing:
CellRanger
Demuxlet
Scater
Seurat
Genome_build: dm6
Supplementary_files_format_and_content: digital gene expression matrix, metadata

molecule: total RNA

organism: Drosophila melanogaster

source_name: L3 brain

extract_protocol:
Brain were dissected in PBS on ice, dissociated in collagenase A/dispase mix. Fixation was performed by adding ice cold MeOH dropwise while slowly mixing.
RNA libraries were prepared for sequencing using standard Illumina and 10x Genomics protocols

tag: fixation

value: MeOH


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G9_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G6_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G7_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G6_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G7_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G7_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G7_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G9_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G6_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G6_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G6_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G6_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G5_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G5_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G8_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G5_ad010


title: fresh L3 brain

value: DGRP-551 based hybrid

value: fresh


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G5_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G5_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G5_ad004


title: WT_Retina

city: Irvine

first: Les

last: Kallestad

address: 829 Health Sciences Rd

department: Ophthalmology

institute: University of California Irvine

data_processing:
cellranger
Genome_build: GRCm38
Supplementary_files_format_and_content: cellranger matrix output

source_name: Retina

extract_protocol:
Fresh retinatissues were harvested from 30 day old C57BL/6J mice according to published protocols. 3 retinas were pooled together to form one biological sample. Under a dissecting microscope, spring scissors were used to puncture the eye and remove the cornea, iris, and lens. The remaining eyecup had 4 radial incisions made every 90 degrees, resulting in a flat and open eye cup. The retina was then gently removed using curved tweezers and placed in a 1.5 ml microcentrifuge tube.
10x Genomic  3' mRNA scRNA  V2


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G4_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G7_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G4_ad001


title: MS2-after 5 Non Responder

city: Ramat Gan

first: Nissan

last: Yissachar

address: Bar-Ilan University

country: Israel

institute: Bar-Ilan University

description:
Countsoutput_MS2.xlsx
MS2_Sample 26-MS2_N427

data_processing:
Reads were trimmed using cutadapt
After trimming, the reads were mapped to the Mouse genome (GRCm38) using STAR  v2.4.2a (using options alignEndsType EndToEnd and outFilterMismatchNoverLmax set to 0.04)
Counting proceeded over genes annotated in Ensembl release 92, using htseq-count (version 0.6.1p1)
Normalization and differential expression was performed using DESeq2 (Version 1.10.1)
Genome_build: GRCm38
Supplementary_files_format_and_content: Processed files include raw counts and DESeq2 normalized counts.

source_name: Colon

extract_protocol:
Colon tissue fragments (3 mm) were stored in RNAlater overnight at 4 °C, homogenized using a bead beater, and bulk RNA was extracted (using RNeasy Plus Universal Mini Qiagen kit). The Illumina TruSeq stranded mRNA library prep kit (#20020594) was used for library generation, and next generation sequencing was performed in the Bar-Ilan University scientific equipment center using the Illumina NextSeq platform (NextSeq 500 High Output v2 kit (#FC-404-2005)).
RNA libraries were prepared for sequencing using standard Illumina protocols

tag: treatment

value: after PA intake

tag: phenotype

value: non responder

tag: tissue

value: Colon

value: 14 days old

tag: disease state

value: multiple sclerosis


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G4_ad007


title: MS2-before 3 Non Responder

description:
Countsoutput_MS2.xlsx
MS2_Sample 23-MS2_N425

value: before PA intake


title: MS2-after 4 Non Responder

description:
Countsoutput_MS2.xlsx
MS2_Sample 25-MS2_N426


title: MS2-before 2 Non Responder

description:
Countsoutput_MS2.xlsx
MS2_Sample 22-MS2_N424


title: MS2-after 6 Responder

description:
Countsoutput_MS2.xlsx
MS2_Sample 21-MS2_N423

value: responder


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G4_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G4_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G4_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G4_ad002


title: MS2-after 5 Responder

description:
Countsoutput_MS2.xlsx
MS2_Sample 20-MS2_N422


title: MS2-before 5 Responder

description:
Countsoutput_MS2.xlsx
MS2_Sample 16-MS2_N420


title: MS2-after 4 Responder

description:
Countsoutput_MS2.xlsx
MS2_Sample 15-MS2_N419


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G3_ad007


title: MS2-after 3 Responder

description:
Countsoutput_MS2.xlsx
MS2_Sample 14-MS2_N418


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G3_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G5_ad008


title: MS2-before 3 Responder

description:
Countsoutput_MS2.xlsx
MS2_Sample 11-MS2_N416


title: MS2-after 2 Non Responder

description:
Countsoutput_MS2.xlsx
MS2_Sample 9-MS2_N414


title: MS2-before 4 Responder

description:
Countsoutput_MS2.xlsx
MS2_Sample 12-MS2_N417


title: MS2-after 3 Non Responder

description:
Countsoutput_MS2.xlsx
MS2_Sample 10-MS2_N415


title: MS2-before 1 Non Responder

description:
Countsoutput_MS2.xlsx
MS2_Sample 7-MS2_N412


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G3_ad010


title: MS2-after 2 Responder

description:
Countsoutput_MS2.xlsx
MS2_Sample 5-MS2_N411


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G2_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G2_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G3_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G3_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G3_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G2_ad010


title: MS2-after 1 Responder

description:
Countsoutput_MS2.xlsx
MS2_Sample 4-MS2_N410


title: MS2-before 6 Responder

description:
Countsoutput_MS2.xlsx
MS2_Sample 17-MS2_N421


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G2_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G2_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G2_ad006


title: MS2-before 2 Responder

description:
Countsoutput_MS2.xlsx
MS2_Sample 3-MS2_N409


title: MS2-after 1 Non Responder

description:
Countsoutput_MS2.xlsx
MS2_Sample 8-MS2_N413


title: MS2-before 1 Responder

description:
Countsoutput_MS2.xlsx
MS2_Sample 2-MS2_N408


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G3_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G2_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G12_ad012


title: MS1-after 3 Non Responder

description:
Countsoutput_MS1.xlsx
MS1_Sample 16

value: 13 days old


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G12_ad010


title: MS1-before 4 Non Responder

description:
Countsoutput_MS1.xlsx
MS1_Sample 14


title: MS1-after 4 Non Responder

description:
Countsoutput_MS1.xlsx
MS1_Sample 15


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G12_ad008


title: MS1-before 3 Non Responder

description:
Countsoutput_MS1.xlsx
MS1_Sample 13


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G12_ad007


title: MS1-after 2 Non Responder

description:
Countsoutput_MS1.xlsx
MS1_Sample 12


title: MS1-after 1 Non Responder

description:
Countsoutput_MS1.xlsx
MS1_Sample 11


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G12_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G12_ad004


title: MS1-before 2 Non Responder

description:
Countsoutput_MS1.xlsx
MS1_Sample 10


title: MS1-before 1 Non Responder

description:
Countsoutput_MS1.xlsx
MS1_Sample 9


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G12_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G11_ad012


title: MS1-before 4 Responder

description:
Countsoutput_MS1.xlsx
MS1_Sample 8


title: MS1-before 1 Responder

description:
Countsoutput_MS1.xlsx
MS1_Sample 5


title: MS1-before 3 Responder

description:
Countsoutput_MS1.xlsx
MS1_Sample 7


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G11_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G11_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G11_ad010


title: MS1-after 3 Responder

description:
Countsoutput_MS1.xlsx
MS1_Sample 3


title: MS1-before 2 Responder

description:
Countsoutput_MS1.xlsx
MS1_Sample 6


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G11_ad008


title: MS1-after 4 Responder

description:
Countsoutput_MS1.xlsx
MS1_Sample 4


title: MS1-after 2 Responder

description:
Countsoutput_MS1.xlsx
MS1_Sample 2


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G11_ad004


title: MS1-after 1 Responder

description:
Countsoutput_MS1.xlsx
MS1_Sample 1


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G11_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G10_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G10_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G10_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G10_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G10_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G12_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G10_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G1_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G1_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G1_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G1_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G1_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G1_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G1_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F9_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F9_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F9_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F9_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F8_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F8_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F9_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F9_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F8_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F8_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F8_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F9_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F8_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G10_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F7_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F8_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F7_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F7_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F7_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F7_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F7_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_G1_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F7_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F6_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F6_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F6_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F6_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F5_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F5_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F5_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F4_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F4_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F4_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F5_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F5_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F4_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F4_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F3_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F4_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F5_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F3_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F3_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F7_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F2_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F2_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F4_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F4_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F3_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F3_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F3_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F2_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F2_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F2_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F2_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F12_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F3_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F12_ad012


title: CEMBA190117_6A_3_CEMBA190117_6A_4_A5_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F12_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F12_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F12_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F12_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F11_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F10_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F11_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F10_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F11_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F11_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F10_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F11_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F11_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F11_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F10_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F1_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F10_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F1_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F1_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F1_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F1_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F2_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F1_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F2_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E9_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E9_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E9_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E9_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F10_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F10_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F10_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E9_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E9_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E8_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E7_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E8_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E7_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E8_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E7_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E7_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E7_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E8_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E7_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E7_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E6_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E7_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E6_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E6_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E6_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E6_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E6_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E6_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E6_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_F1_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E5_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E5_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E5_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E5_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E8_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E8_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E8_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E5_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E5_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E5_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E4_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E4_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E4_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E4_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E4_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E4_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E4_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E3_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E4_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E3_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E3_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E3_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E3_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E3_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E2_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E3_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E2_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E2_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E2_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E2_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E3_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E12_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E2_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E12_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E12_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E12_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E12_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E11_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E11_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E12_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E11_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E2_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E10_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E10_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E11_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E10_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E10_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E10_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E10_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E1_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E1_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E12_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E1_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E1_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E1_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E11_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E11_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D9_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D9_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D9_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E10_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E10_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D9_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D9_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D9_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D9_ad002


title: Col-0 28 rep3

city: Austin

first: Enamul

last: Huq

state: TX

address: Department of MBS, NHB 2.606, Stop A5000, 100 East 24th St.

institute: The University of Texas at Austin

data_processing:
FastQC
Bowtie2
Tophat
Htseq
EdgeR

organism: Arabidopsis thaliana

source_name: seedlings

growth_protocol: Total two genotypes; Col-0, spaQ plants were used for mRNA sequencing analysis.

extract_protocol:
Total RNA was isolated using sigma plant total RNA kit(Sigma, Cat.# STRN250).
3'Tag-Seq method article was used to generate TagSeq library according to the manufacturer’s instruction. (doi: 10.1111/1755-0998.12529)
3'Tag-RNA-seq

treatment_protocol: The seeds were surface sterilized and plated on the MS media without sucrose, cold stratified for three days in dark to induce germination. After three days, seeds were further treated 5 days in 22degC with white light (25 µmole/s2/m2). After 5 days, seedlings were either treated with 4 hour of continuous white light (25 µmole/s2/m2) in 22.

tag: temperature

tag: developmental stage

value: 6 days old seedlings

tag: genotype


title: spaQ 28 rep1

value: spaQ


title: spaQ 28 rep2


title: spaQ 28 rep3


title: Col-0 28 rep1


title: spaQ 22 rep3


title: Col-0 28 rep2


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D8_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_E1_ad007


title: spaQ 22 rep2


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D8_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D9_ad008


title: spaQ 22 rep1


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D8_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D8_ad007


title: Col-0 22 rep3


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D8_ad002


title: Col-0 22 rep2


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D8_ad008


title: Col-0 22 rep1


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D7_ad012


title: input DNA

city: Beijing

first: Fan

last: Zhao

address: Qinghuayuan

country: China

institute: Tsinghua University

data_processing:
ChIP-seq reads were aligned to the hg38 genome using Bowtie2
sam files were sorted and converted to bam files using samtools
Peak calling using MACS2 callpeak with the following setting: -f BAMPE, for chip-seq data of histone using paramater -- broad --broad-cutoff 0.1
Genome_build: hg38
Supplementary_files_format_and_content: bigwig files were generated using bamCoverage of deeptools

organism: Homo sapiens

source_name: Primary human embryonic kidney cells

extract_protocol:
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody.
Libraries were prepared according to Illumina's instructions accompanying the VAHTS Universal DNA Library Prep Kit (ND607). Briefly, DNA was subjected to end preparation, adapter ligation and library amplification.

treatment_protocol: HEK293 cells were transiently transfected with Flag-Spindlin1 using Liposome3000

tag: cell line

tag: cell type

value: Human embryonic kidney 293 cells

tag: chip antibody


title: FLAG-Spindlin1_ChIPSeq

value: FLAG (Sigma, A8592)


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D7_ad007

value: ACA,PL,ILA


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D7_ad001

value: male


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D7_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D7_ad010


title: H3K4me3_ChIPSeq

value: H3K4me3 (Millipore, 07-473)


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D7_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D6_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D6_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D6_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D6_ad006


title: Control rep1 for siNKX2-2 or siTCF4 RNAseq

city: Shenzhen

first: Yueyuan

last: Zheng

state: China

address: Zhenyuan Road, 628

institute: The Seventh Affiliated Hospital of Sun Yat-sen University

description:
A673_NC_batch2_rep1
processed data file: A673_siNKX22_vs_control_DEG.txt
processed data file: A673_siTCF4_vs_control_DEG.txt

data_processing:
For ChIP-seq data, raw reads were aligned to hg19 reference genome using bowtie 2 aligner (version 2.3.4.3) followed by removal of PCR duplicates with Picard MarkDuplicates. ChIP-seq peaks were called using MACS (Model-Based Analysis of ChIP-seq, version 2.1.2) with default parameters.
Reads were extended in the 5’ to 3’ direction by the estimated fragment length and normalized at -log10 of the Poisson p-value of IP file compared to expected background counts by MACS2 bdgcmp command.
BigWig files were generated by bedGraphToBigWig tool (https://genome.ucsc.edu/) and visualized in Integrative Genomics Viewer (http://software.broadinstitute.org/software/igv/). Motif identification and comparison were performed with HOMER using findMotifsGenome program.
For RNAseq data, we aligned 150bp paired-end reads to hg19 (UCSC) genome using HISAT2 (version 2.0.4) and counted reads with htseq-count program (version 0.11.2). Differentially expressed genes were identified by DESeq2 package with adjusted P value < 0.1 and absolute log2 (Fold change) > 0.5.
Genome_build: hg19 (GRCh37)
Supplementary_files_format_and_content: bigwig files representing signal normalized to per million reads.
Supplementary_files_format_and_content: narrowPeak files
Supplementary_files_format_and_content: *DEG.txt: Tab-delimited text files

source_name: Ewing sarcoma cell line

growth_protocol: A673 cells were grown in Dulbecco’s Modified Eagle Medium (DMEM) containing 10% fetal bovine serum (FBS), 100 U/ml penicillin and 100 mg/ml streptomycin, and kept in a humidified incubator at 37 °C with 5% CO2.

extract_protocol:
RNAs were isolated using miRNeasy RNA isolation kit (Qiagen). RNA sequencing libraries were prepared using TruSeq Library Prep Kit (Illumina) according to the manufacturer’s protocol, and were sequenced on HiSeq sequencer (Illumina).
A673 cell lysates were clarified from sonicated nuclei and TF-DNA complexes were isolated with following antibody: Anti-KLF15 (Proteintech, 66185-1-Ig), anti-TCF4 (Abcam, ab223073), anti-NKX2-2 (Proteintech, 13013-1-AP). Immunoprecipitated DNA was extracted with the Min-Elute PCR purification kit (Qiagen), followed by DNA library preparation using standard Illumina protocol and sequencing on HiSeq 4000 platform (Illumina).

treatment_protocol: A673 cells were transfected with siRNA targeting KLF15, TCF4 or NKX2-2 for 24 hours using Lipofectamine RNAiMAX transfection reagent (Invitrogen).

value: Ewing sarcoma cell line

tag: sirna

value: Control


title: mPBS1-4

city: Rochester

first: Xuewei

last: Wang

address: 200 1st street

institute: Mayo Clinic

data_processing:
Base calling by CASAVA
Alignment by STAR
Gene count by FeatureCount
Genome_build: mm10
Supplementary_files_format_and_content: merged_gene_count.csv

source_name: mPBS1_skeletal muscle

growth_protocol: 7-week old male or female C57BL6  mice were studied following injection of tumor cells (LLC1) or vehicle (PBS).

extract_protocol:
RNA was prepared using RNA/protein spin columns (Clontech).
RNA libraries were prepared with a TruSeq RNA Sample Prep Kit version 2 (Illumina). Reverse transcription and adaptor ligation steps were performed manually. Poly(A) mRNA was purified from total RNA using oligo(dT) magnetic beads. RNA was treated with RNase-free DNase during preparation of RNAs using the Nucleospin RNA/Protein kit (Clontech). Purified mRNA was fragmented at 95 °C for 8 min, eluted from the beads, and primed for first strand cDNA synthesis. The RNA fragments were then copied into first strand cDNA using SuperScript III reverse transcriptase and random primers (Invitrogen). Second strand cDNA synthesis was performed using DNA polymerase I and RNase H. The double-stranded cDNA was purified using a single AMPure XP bead (Agencourt) clean-up step. The cDNA ends were repaired and phosphorylated using Klenow, T4 polymerase, and T4 polynucleotide kinase followed by AMPure XP bead clean-up. The blunt-ended cDNAs were modified to include a single 3′-adenylate (A) residue using Klenow exo− (3′–5′ exo minus). Paired end DNA adaptors (Illumina) with a single “T” base overhang at the 3′-end were immediately ligated to the “A-tailed” cDNA population. Unique indexes, included in the standard TruSeq Kits (12-Set A and 12-Set B) were incorporated at the adaptor ligation step for multiplex sample loading on the flow cells. The adaptor-modified DNA fragments were enriched by 12 cycles of PCR using primers included in the Illumina Sample Prep Kit. The concentration and size distribution of the libraries were determined on an Agilent Bioanalyzer DNA 1000 chip (Santa Clara, CA).

treatment_protocol: Following subcutaneous injection of tumor cells or vehicle, tumor cells were allowed to grow for 3 weeks.  The weight of the mice was assessed periodically.

value: subcutaneous injections of vehicle

value: gastrocnemius muscle


title: A549, parental

city: Gyeonggi-do

first: Hyonchol

last: Jang

address: 323, Ilsan-ro, Ilsandong-gu, Goyang-si

country: South Korea

institute: National Cancer Center

data_processing:
bcl2fastq v1.8.4 package was used for basecalling.
Trimmomatic program is used to remove adapter sequences and bases with base quality lower than three from the ends. Also using sliding window method, bases of reads that does not qualify for window size 4, and mean quality 15 are trimmed. Afterwards, reads with length shorter than 36bp are dropped to produce trimmed data.
Sequenced reads were mapped to GRCh38 whole genome by using HISAT2 version 2.1.0 with parameter -p 20 -dta --rna-strandness RF --novel-splicesite-outfile -x -1 -2
Fragment Per Kilobase of exon per Megabase of library size (FPKM) were calculated by using StringTie version 2.1.3b, with parameter --rf -p 20 -l -o -G -A -C -e
Genome_build: GRCh38
Supplementary_files_format_and_content: tab-delimited text file includes Read_Count values, FPKM values, TPM values for each Sample

molecule: polyA RNA

source_name: Lung cancer cell  (control)

extract_protocol:
RNAs were extracted from A549 cells. Poly(A) mRNA using the Illumina TruSeq RNA Sample Prep Kit, according to the manufacturer’s instructions.
Libraries were prepared using Illumina TruSeq mRNA Sample Prep kit, according to the manufacturer's protocol.

value: Lung

value: control


title: A549, cisplatin-resistant (CR)

source_name: Lung cancer cells that survived 7 days after 3 days of cisplatin treatment

value: cisplatin


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D6_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D6_ad008


title: Control rep2 for siNKX2-2 or siTCF4 RNAseq

description:
A673_NC_batch2_rep2
processed data file: A673_siNKX22_vs_control_DEG.txt
processed data file: A673_siTCF4_vs_control_DEG.txt


title: A673 siTCF4 rep2 RNAseq

description:
A673_siTCF4_rep2
processed data file: A673_siTCF4_vs_control_DEG.txt


title: mPBS1-2


title: mPBS2-5

source_name: mPBS2_skeletal muscle


title: A673 siTCF4 rep1 RNAseq

description:
A673_siTCF4_rep1
processed data file: A673_siTCF4_vs_control_DEG.txt


title: H3K9me3_ChIPSeq

value: H3K9me3 (Abcam, ab8898)


title: mPBS1-1


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D5_ad012


title: mPBS2-4


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D7_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D5_ad001


title: mPBS1-3


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D5_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D7_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D5_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D6_ad007


title: mLLC2-5

source_name: mLLC2_skeletal muscle

value: subcutaneous injections of LLC1 lung adenocarcinoma cells


title: A673 siNKX2-2 rep2 RNAseq

description:
A673_siNKX2-2_rep2
processed data file: A673_siNKX22_vs_control_DEG.txt


title: mLLC2-4


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D5_ad006


title: A673 siNKX2-2 rep1 RNAseq

description:
A673_siNKX2-2_rep1
processed data file: A673_siNKX22_vs_control_DEG.txt


title: Control rep2 for siKLF15 RNAseq

description:
A673_NC_batch1_rep2
processed data file: A673_siKLF15_vs_control_DEG.txt


title: mLLC2-2


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D4_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D5_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D5_ad004


title: Control rep1 for siKLF15 RNAseq

description:
A673_NC_batch1_rep1
processed data file: A673_siKLF15_vs_control_DEG.txt


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D4_ad012


title: A673 siKLF15 rep2 RNAseq

description:
A673_siKLF15_rep2
processed data file: A673_siKLF15_vs_control_DEG.txt


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D4_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D4_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D4_ad004


title: A673 siKLF15 rep1 RNAseq

description:
A673_siKLF15_rep1
processed data file: A673_siKLF15_vs_control_DEG.txt


title: mLLC1-5

source_name: mLLC1_skeletal muscle


title: mLLC2-1


title: mLLC1-4


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D4_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D4_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D3_ad012


title: Old HFD 4 Methylseq

city: New York

first: Donald

middle: K

last: Scott

state: New York

address: 1428 Madison Avenue

department: Diabetes Obesity and Metabolism Institute

institute: MSSM

description: Methylseq

data_processing:
>> RNA-Seq <<
Reads were aligned to the mm10 mouse reference using STAR aligner (v2.4.2a)
Quantification of genes annotated in Gencode vM5 was performed using featureCounts (v1.4.3)
Quantification of transcripts used Kalisto
QC were collected with Picard (v1.83) and RSeQC
Normalization of feature counts and differential expression analysis was done using the DESeq2 package
Genome_build: mm10
Supplementary_files_format_and_content: count and DEseq2 tab-delimited text files
>> Methyl-seq<<
Trim raw reads for quality Package: Trimmomatic Tool: illuminaclip
Map sequencing reads to mm10 Package: BSMAP Tool: BSMAP
Calculate percent methylation at CpGs Package: BSMAP Tool: methratio.py
Split by strand Package: BEDtools Tool: Split
Merge & sort by strand Package: BEDtools Tool: Merge
Remove Duplicates Package: Picard Tool: MarkDuplicates
Merge strands Package: BEDtools Tool: Merge
Filter for properly paired reads Package: Picard Tool: MarkDuplicates
Clip overlapping reads Package: BSMAP Tool: methratio.py
Genome_build: mm10
Supplementary_files_format_and_content: compressed tab-delimited methyl call txt files

source_name: Islets

growth_protocol: C57/BL6J mice

extract_protocol: Islets were isolated, DNA was isolated,

treatment_protocol: Male mice 8 weeks or 52 weeks old were put on diets of 41%  or 13.1 % kcal from fat diet for 1 week

tag: age group

value: Old

tag: diet

value: high fat diet

value: Islets

value: 1 year

value: wild type


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D3_ad001

tag: brain region

value: ACA,PL,ILA

tag: Sex


title: Old HFD 2 Methylseq

description: Methylseq

tag: strain

tag: age


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D3_ad007

value: male


title: fPBS2-2

source_name: fPBS2_skeletal muscle

value: female

tag: treatment


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D3_ad006


title: Old HFD 1 Methylseq

description: Methylseq


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D3_ad010


title: Old SD 4 Methylseq

description: Methylseq

value: standard diet


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D3_ad004


title: Old SD 3 Methylseq

description: Methylseq


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D3_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D2_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D3_ad008


title: fPBS1-4

source_name: fPBS1_skeletal muscle


title: fPBS1-3


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D2_ad010


title: fPBS2-1


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D2_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D2_ad007


title: Old SD 1 Methylseq

description: Methylseq


title: fPBS2-3


title: Young HFD 4 Methylseq

description: Methylseq

value: Young

value: 8-10 weeks


title: fLLC2-5

source_name: fLLC2_skeletal muscle

value: gastrocnemius muscle


title: fLLC2-4


title: Young HFD 3 Methylseq

description: Methylseq


title: fLLC2-3


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D2_ad008

source_name: C57BL/6J Male P56 Mouse Brain


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D12_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D2_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D12_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D12_ad012


title: Young HFD 2 Methylseq

description: Methylseq


title: Old SD 2 Methylseq

description: Methylseq


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D12_ad006


title: fLLC2-1


title: Young HFD 1 Methylseq

description: Methylseq


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D12_ad004


title: Young RD 4 Methylseq

description: Methylseq


title: fLLC1-5

source_name: fLLC1_skeletal muscle


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D12_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D11_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D11_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D12_ad002


title: Young RD 3 Methylseq

description: Methylseq


title: fLLC1-4


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D11_ad007


title: Young RD 2 Methylseq

description: Methylseq


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D11_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D2_ad004


title: DKMR55_II_WT_20hr_rep3

city: Marseille

first: Rudy

last: AUSSEL

address: Parc Scientifique et Technologique de Luminy

country: France

institute: CIML

description:
Sample 53
SAMP37_to_SAMP54_featureCounts_table

data_processing:
Image analysis and base calling were performed using RTA 2.7.3 and bcl2fastq 2.17.1.14.
fastq files were aligned to mouse reference genome GRCm38 using STAR aligner.
BAM files resulting from the alignments were summarized to genes using the featureCounts algorithm
Expression data was normalized using the VST function in the DESeq2 package in R
Differentially expressed genes (³1-fold and £5% false discovery rate) were identified by linear modeling using the DESeq2 package
Genome_build: mm10 (GRCm38)
Supplementary_files_format_and_content: tab-delimited text files with counts for each Sample from featureCounts

extract_protocol:
Total RNA  was extracted by using RNeasy mini plus kit (QIAGEN)
RNA-Seq libraries were generated from 250 ng of total RNA using TruSeq Stranded mRNA Library Prep Kit and TruSeq RNA Single Indexes kits A and B (Illumina, San Diego, CA), according to manufacturer's instructions. Briefly, following purification with poly-T oligo attached magnetic beads, the mRNA was fragmented using divalent cations at 94oC for 2 minutes. The cleaved RNA fragments were copied into first strand cDNA using reverse transcriptase and random primers. Strand specificity was achieved by replacing dTTP with dUTP during second strand cDNA synthesis using DNA Polymerase I and RNase H. Following addition of a single 'A' base and subsequent ligation of the adapter on double stranded cDNA fragments, the products were purified and enriched with PCR (30 sec at 98oC; [10 sec at 98oC, 30 sec at 60oC, 30 sec at 72oC] x 12 cycles; 5 min at 72oC) to create the cDNA library. Surplus PCR primers were further removed by purification using AMPure XP beads (Beckman-Coulter, Villepinte, France) and the final cDNA libraries were checked for quality and quantified using capillary electrophoresis.

treatment_protocol: Purified naïve CD4+ T cells were stimulated with plate-bound anti-CD3 (3 microgram/mL) plus anti-CD28 (1 microgram/mL) for 4 h and 20 h. Total RNA of untreated samples was extracted immediately after purification.

tag: background strain

value: wild-type (hDTR+)

value: anti-CD3 and anti-CD28 stimulation for 20hr

value: lymph nodes and spleens

value: CD4+ T cells from lymph nodes and spleens


title: Young RD 1 Methylseq

description: Methylseq

tag: tissue


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D11_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D11_ad004


title: DKMR56_II_LatKO_20hr_rep3

description:
Sample 54
SAMP37_to_SAMP54_featureCounts_table

value: Lat-/- (hDTR-)


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D11_ad001


title: DKMR53_II_WT_4hr_rep3

description:
Sample 51
SAMP37_to_SAMP54_featureCounts_table

value: anti-CD3 and anti-CD28 stimulation for 4hr


title: DKMR54_II_LatKO_4hr_rep3

description:
Sample 52
SAMP37_to_SAMP54_featureCounts_table


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D10_ad008


title: DKMR52_II_LatKO_unstim_rep3

description:
Sample 50
SAMP37_to_SAMP54_featureCounts_table

value: untreated


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D10_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D10_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D10_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D10_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D11_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D10_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D10_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D10_ad004


title: DKMR51_II_WT_unstim_rep3

description:
Sample 49
SAMP37_to_SAMP54_featureCounts_table


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D1_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D1_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D1_ad007


title: DKMR48_II_LatKO_4hr_rep2

description:
Sample 46
SAMP37_to_SAMP54_featureCounts_table


title: DKMR50_II_LatKO_20hr_rep2

description:
Sample 48
SAMP37_to_SAMP54_featureCounts_table


title: DKMR47_II_WT_4hr_rep2

description:
Sample 45
SAMP37_to_SAMP54_featureCounts_table


title: DKMR49_II_WT_20hr_rep2

description:
Sample 47
SAMP37_to_SAMP54_featureCounts_table


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D1_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D1_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C9_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C9_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C9_ad010


title: DKMR45_II_WT_unstim_rep2

description:
Sample 43
SAMP37_to_SAMP54_featureCounts_table


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C9_ad012


title: DKMR44_II_LatKO_20hr_rep1

description:
Sample 42
SAMP37_to_SAMP54_featureCounts_table


title: CEMBA190117_6A_1_CEMBA190117_6A_2_D1_ad006


title: MOLM14, shCTRL, 4

type: RNA

city: Strassen

first: Tony

last: KAOMA

address: 1 A-B Rue Thomas Edison

department: Quantitative Biology Unit

country: Luxembourg

institute: Luxembourg Institute of Health

hyb_protocol: cRNA products were randomly fragmented and labeled prior hybridization onto Affymetrix GeneChips.

scan_protocol: GeneChips were scanned using GeneChip Scanner 3000

data_processing: CEL files were preprocessed into R software with rma function of oligo package

label: biotin

source_name: MOLM14 transfected with a scrambled shRNA , technical replicate 4

label_protocol: 100 ng of total RNAs were reverse transcribed into cDNA, then transcribed into cRNAs and labeled into biotinylated cRNA using the GeneChip WT PLUS Reagent kit (Affymetrix) according to the manufacturer’s standard protocols (P/N 703174 Rev.2 )

growth_protocol: Human MOLM14 AML cel lines was maintained in Roswell Park Memorial Institute (RPMI) 1640 supplemented with 10% fetal bovine serum (Invitrogen, Carlsbad, CA, USA) in the presence of 100 units per ml of penicillin and 100 μg/ml of streptomycin, and were incubated at 37°C with 5% CO2. The cultured cells were split every 2–3 days and maintained in an exponential growth phase.

extract_protocol: Total RNAs were extracted from AML clones expressing MOLM-14 sh-CTL and MOLM14-shCD39 gene using the Qiagen kit according to the manufacturer’s instructions

treatment_protocol: treated with doxycycline during 72 hours

tag: shRNA

value: shCTRL


title: MOLM14, shCTRL, 2

source_name: MOLM14 transfected with a scrambled shRNA , technical replicate 2


title: DKMR43_II_WT_20hr_rep1

description:
Sample 41
SAMP37_to_SAMP54_featureCounts_table


title: MOLM14, shCTRL, 3

source_name: MOLM14 transfected with a scrambled shRNA , technical replicate 3


title: DKMR46_II_LatKO_unstim_rep2

description:
Sample 44
SAMP37_to_SAMP54_featureCounts_table


title: MOLM14, shCD39, 3

source_name: MOLM14 transfected with shRNA against ENTPD1/CD39, technical replicate 3


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C9_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C9_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C9_ad004


title: MOLM14, shCTRL, 1

source_name: MOLM14 transfected with a scrambled shRNA , technical replicate 1


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C9_ad008


title: MOLM14, shCD39, 1

source_name: MOLM14 transfected with shRNA against ENTPD1/CD39, technical replicate 1


title: DKMR42_II_LatKO_4hr_rep1

description:
Sample 40
SAMP37_to_SAMP54_featureCounts_table


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C8_ad012


title: DKMR41_II_WT_4hr_rep1

description:
Sample 39
SAMP37_to_SAMP54_featureCounts_table


title: Pancreatic_islets_wild-type_mice_rep3

city: Neuherberg

first: Johannes

last: Beckers

address: Ingolstaedter Landstr. 1

department: Institute of Experimental Genetics

country: Germany

institute: Helmholtz Zentrum Muenchen

description: Gene expression data from isolated and cultured pancreatic islets from wild-type mice, biological replicate 3

hyb_protocol: According to the Affymetrix expression protocol (HWS kit)

data_processing: RMA probeset summary with standard settings of the Transcriptome Analysis Console (4.0.1.36; Thermofisher Scientific)

source_name: Isolated and cultured pancreatic islets from wild-type mice, biological replicate 3

label_protocol: Ovation PicoSL WTA System V2 and Encore Biotin Module (Nugen)

growth_protocol: Animals were housed under spf conditions

extract_protocol: RNeasy Plus Micro kit (Qiagen)

treatment_protocol: Pancreatic islets were isolated and cultured overnight in islet medium containing RPMI solution with 11.1 mM glucose, 10% v/v FBS and 1% v/v penicillin/streptomycin.

value: 10 weeks

tag: gender

value: WT


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C8_ad007

value: ACA,PL,ILA


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C8_ad010

value: male


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C8_ad006


title: DKMR40_II_LatKO_unstim_rep1

description:
Sample 38
SAMP37_to_SAMP54_featureCounts_table


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C8_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C8_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C8_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C8_ad001


title: MOLM14, shCD39, 4

source_name: MOLM14 transfected with shRNA against ENTPD1/CD39, technical replicate 4


title: Pancreatic_islets_Pax6-Leca2_mice_rep3

description: Gene expression data from isolated and cultured pancreatic islets from homozygous Pax6_Leca2 mice, biological replicate 3

scan_protocol: According to the Affymetrix expression protocol

source_name: Isolated and cultured pancreatic islets from homozygous Pax6_Leca2 mice, biological replicate 3


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C7_ad012


title: Pancreatic_islets_Pax6-Leca2_mice_rep4

description: Gene expression data from isolated and cultured pancreatic islets from homozygous Pax6_Leca2 mice, biological replicate 4

source_name: Isolated and cultured pancreatic islets from homozygous Pax6_Leca2 mice, biological replicate 4


title: MOLM14, shCD39, 2

source_name: MOLM14 transfected with shRNA against ENTPD1/CD39, technical replicate 2


title: Pancreatic_islets_wild-type_mice_rep4

description: Gene expression data from isolated and cultured pancreatic islets from wild-type mice, biological replicate 4

source_name: Isolated and cultured pancreatic islets from wild-type mice, biological replicate 4


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C7_ad001


title: DKMR35_I_dKO_20hr_rep3

description:
Sample 34
SAMP1_to_SAMP36_featureCounts_table


title: DKMR36_I_CD5KO_20hr_rep3

description:
Sample 35
SAMP1_to_SAMP36_featureCounts_table


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C7_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C7_ad002


title: DKMR34_I_WT_20hr_rep3

description:
Sample 33
SAMP1_to_SAMP36_featureCounts_table

value: wild-type


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C7_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C7_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C7_ad008


title: DKMR32_I_CD6KO_4hr_rep3

description:
Sample 32
SAMP1_to_SAMP36_featureCounts_table


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C6_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C6_ad007


title: Pancreatic_islets_wild-type_mice_rep1

description: Gene expression data from isolated and cultured pancreatic islets from wild-type mice, biological replicate 1

source_name: Isolated and cultured pancreatic islets from wild-type mice, biological replicate 1


title: Pancreatic_islets_Pax6-Leca2_mice_rep2

description: Gene expression data from isolated and cultured pancreatic islets from homozygous Pax6_Leca2 mice, biological replicate 2

source_name: Isolated and cultured pancreatic islets from homozygous Pax6_Leca2 mice, biological replicate 2


title: DKMR39_II_WT_unstim_rep1

description:
Sample 37
SAMP37_to_SAMP54_featureCounts_table


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C6_ad002


title: DKMR37_I_CD6KO_20hr_rep3

description:
Sample 36
SAMP1_to_SAMP36_featureCounts_table


title: DKMR31_I_CD5KO_4hr_rep3

description:
Sample 31
SAMP1_to_SAMP36_featureCounts_table


title: DKMR30_I_dKO_4hr_rep3

description:
Sample 30
SAMP1_to_SAMP36_featureCounts_table


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C5_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C5_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C6_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C5_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C5_ad010


title: Pancreatic_islets_Pax6-Leca2_mice_rep1

description: Gene expression data from isolated and cultured pancreatic islets from homozygous Pax6_Leca2 mice, biological replicate 1

source_name: Isolated and cultured pancreatic islets from homozygous Pax6_Leca2 mice, biological replicate 1


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C5_ad004

source_name: C57BL/6J Male P56 Mouse Brain


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C5_ad002


title: Pancreatic_islets_wild-type_mice_rep2

description: Gene expression data from isolated and cultured pancreatic islets from wild-type mice, biological replicate 2

source_name: Isolated and cultured pancreatic islets from wild-type mice, biological replicate 2


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C5_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C4_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C4_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C4_ad010


title: DKMR26_I_dKO_unstim_rep3

description:
Sample 26
SAMP1_to_SAMP36_featureCounts_table


title: DKMR25_I_WT_unstim_rep3

description:
Sample 25
SAMP1_to_SAMP36_featureCounts_table


title: DKMR29_I_WT_4hr_rep3

description:
Sample 29
SAMP1_to_SAMP36_featureCounts_table


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C4_ad008


title: DKMR28_I_CD6KO_unstim_rep3

description:
Sample 28
SAMP1_to_SAMP36_featureCounts_table


title: PV_terminal_PrD_1

city: Zurich

first: Claudia

last: Scheckel

state: NY

address: Schmelzbergstrasse 12

department: Institute of Neuropathology

country: Switzerland

institute: University Hospital Zurich

description: GeneCounts.txt

data_processing:
Library strategy: Ribo-seq
filtering (min:0-13:20,mean:14-39:20)
clipping of 3'linker sequence (GTGTCAGTCACTTCCAGCGG)
collapsing of exact sequences
removal of 5'linker (14nt)
alignment with STAR
Genome_build: Ensembl GRCm38.p5
Supplementary_files_format_and_content: Txt file containing uniquely and unambigously mapped reads per gene (column 3 of GeneCounts output of STAR)

source_name: hemisphere

growth_protocol: Dissected flash-frozen brain regions or hemispheres from control or prion diseased mice

extract_protocol:
Partial RNase digestion (MNase treatment) and isolation of ribosome protected fragments (GFP immunoprecipitation followed by ultracentrifugation)
CIP treatment, 3'linker ligation, reverse transcription, circularization and PCR ampflication

treatment_protocol: Intraperitoneal inoculation of non-infectious brain homogenate or prions

tag: cre driver

value: PV

tag: time point

value: terminal

value: prions

value: F

tag: multiplex index

value: ATCG


title: PV_terminal_PrD_3

description: GeneCounts.txt

value: M

value: GATC


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C3_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C5_ad007


title: PV_terminal_ctrl_3

description: GeneCounts.txt

value: non-infectious brain homogenate

value: AGCT


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C3_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C3_ad001


title: DKMR27_I_CD5KO_unstim_rep3

description:
Sample 27
SAMP1_to_SAMP36_featureCounts_table

tag: genotype

value: lymph nodes and spleens

tag: cell type

value: CD4+ T cells from lymph nodes and spleens


title: PV_terminal_ctrl_2

description: GeneCounts.txt

value: TACG


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C4_ad002


title: DKMR23_I_CD5KO_20hr_rep2

description:
Sample 23
SAMP1_to_SAMP36_featureCounts_table

value: anti-CD3 and anti-CD28 stimulation for 20hr


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C3_ad010


title: fPBS1-1

source_name: fPBS1_skeletal muscle

value: female

value: subcutaneous injections of vehicle

value: gastrocnemius muscle


title: PV_terminal_ctrl_1

description: GeneCounts.txt

value: GCTA


title: DKMR21_I_WT_20hr_rep2

description:
Sample 21
SAMP1_to_SAMP36_featureCounts_table

value: wild-type


title: PV_terminal_PrD_2

description: GeneCounts.txt

value: PV

value: terminal

value: TCGA


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C3_ad004

value: ACA,PL,ILA

value: male


title: DKMR24_I_CD6KO_20hr_rep2

description:
Sample 24
SAMP1_to_SAMP36_featureCounts_table


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C4_ad012


title: DKMR22_I_dKO_20hr_rep2

description:
Sample 22
SAMP1_to_SAMP36_featureCounts_table


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C3_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C3_ad008


title: PV_8wpi_PrD_3

description: GeneCounts.txt

value: prions

value: F

value: CTAG


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C2_ad008


title: PV_8wpi_PrD_2

description: GeneCounts.txt

value: M

value: GATC


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C2_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C2_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C2_ad007


title: PV_8wpi_ctrl_3

description: GeneCounts.txt

value: non-infectious brain homogenate


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C2_ad006


title: DKMR19_I_CD5KO_4hr_rep2

description:
Sample 19
SAMP1_to_SAMP36_featureCounts_table

value: anti-CD3 and anti-CD28 stimulation for 4hr

value: lymph nodes and spleens

value: CD4+ T cells from lymph nodes and spleens


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C2_ad012


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C2_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C12_ad012


title: DKMR18_I_dKO_4hr_rep2

description:
Sample 18
SAMP1_to_SAMP36_featureCounts_table


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C12_ad007


title: DKMR16_I_CD6KO_unstim_rep2

description:
Sample 16
SAMP1_to_SAMP36_featureCounts_table

value: untreated


title: PV_8wpi_ctrl_1

description: GeneCounts.txt

value: ATCG


title: PV_4wpi_PrD_3

description: GeneCounts.txt


title: DKMR14_I_dKO_unstim_rep2

description:
Sample 14
SAMP1_to_SAMP36_featureCounts_table


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C12_ad010


title: DKMR20_I_CD6KO_4hr_rep2

description:
Sample 20
SAMP1_to_SAMP36_featureCounts_table


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C12_ad008


title: PV_4wpi_PrD_2

description: GeneCounts.txt


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C12_ad004


title: DKMR13_I_WT_unstim_rep2

description:
Sample 13
SAMP1_to_SAMP36_featureCounts_table

value: wild-type


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C12_ad001


title: DKMR17_I_WT_4hr_rep2

description:
Sample 17
SAMP1_to_SAMP36_featureCounts_table


title: PV_8wpi_ctrl_2

description: GeneCounts.txt

value: PV

value: GCTA


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C11_ad012


title: DKMR12_I_CD6KO_20hr_rep1

description:
Sample 12
SAMP1_to_SAMP36_featureCounts_table

value: anti-CD3 and anti-CD28 stimulation for 20hr


title: PV_4wpi_PrD_1

description: GeneCounts.txt

value: AGCT


title: PV_4wpi_ctrl_3

description: GeneCounts.txt


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C11_ad008

value: ACA,PL,ILA

value: male


title: DKMR11_I_CD5KO_20hr_rep1

description:
Sample 11
SAMP1_to_SAMP36_featureCounts_table


title: PV_4wpi_ctrl_2

description: GeneCounts.txt

value: F

value: TAGC


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C11_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C11_ad006


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C12_ad006


title: DKMR10_I_dKO_20hr_rep1

description:
Sample 10
SAMP1_to_SAMP36_featureCounts_table


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C11_ad004


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C11_ad002


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C10_ad012


title: PV_4wpi_ctrl_1

description: GeneCounts.txt

value: M

value: CGAT


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C12_ad002


title: PV_2wpi_PrD_3

description: GeneCounts.txt

value: prions


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C11_ad001


title: PV_2wpi_PrD_2

description: GeneCounts.txt

value: GATC


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C10_ad010


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C10_ad007


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C10_ad008


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C11_ad010


title: PV_2wpi_PrD_1

description: GeneCounts.txt

value: CTAG


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C10_ad006


title: PV_2wpi_ctrl_3

description: GeneCounts.txt

value: non-infectious brain homogenate

value: TCGA


title: DKMR09_I_WT_20hr_rep1

description:
Sample 9
SAMP1_to_SAMP36_featureCounts_table

value: lymph nodes and spleens

value: CD4+ T cells from lymph nodes and spleens


title: DKMR08_I_CD6KO_4hr_rep1

description:
Sample 8
SAMP1_to_SAMP36_featureCounts_table

value: anti-CD3 and anti-CD28 stimulation for 4hr


title: DKMR07_I_CD5KO_4hr_rep1

description:
Sample 7
SAMP1_to_SAMP36_featureCounts_table


title: DKMR05_I_WT_4hr_rep1

description:
Sample 5
SAMP1_to_SAMP36_featureCounts_table

value: wild-type


title: HT_HL_End_3

city: Beaucouze

first: Julia

last: Buitink

address: 42 rue Georges Morel

department: BAP

institute: INRAE

data_processing:
Clean reads were mapped to the reference genome tomato SL4.0 using SALMON to calculate gene expression level
Genome_build: SL4.0
Supplementary_files_format_and_content: tab-delimited text file with count values for every gene and every sample

organism: Solanum lycopersicum

source_name: High temperature_High light_endosperm

growth_protocol: Plants were grown under controlled greenhouse conditions in 10 L pots containing substrate (Irish peat, perlite, coconut fiber; 50/40/10; v/v/v), watered with a nutrient solution and supplemented with 16 h of 250 µmol m2 s-1 light. The day and night temperatures were maintained at > 23°C/20°C. Breaker fruits (i.e 63 DAF) were excised and transfered to growth chambers for 7 days under 4 different environments : standard temperature (ST, 23°C days/ 20°C night) + high light (HL, 16h 300µE) ; standard temperature (ST) + dim light (DL, 16h 25µE); high temperature (HT, 32°C day/26°C night) + high light (HL) or high temperature (HT) + dim light (DL).

extract_protocol:
Total RNAs were extracted from the frozen grinded seed tissues using the NucleoSpin® RNA Plant and Fungi kit (Macherey-Nagel, Düren, Germany), according to the manufacturer instructions (protocol 5.1) without the incubation step at 56°C using the following recommended sample type: alfalfa seed for embryo and potato tuber for the endosperm
Samples were sent to BGI, Hong Kong, for library preparation. Libraries were constructed following a custom protocol from samples that passed quality controls (mass > 2µg, concentration>80ng/microl, OD260/280 ~= 2.00, OD260/230 ~= 2.20, RIN>6.5, 28S/18S<1.0, baseline smooth). After mRNA enrichment, RNA was fragmented and reverse transcribed to double-strand cDNA (dscDNA) by N6 random primer. The synthesized cDNA was subjected to end-repair and then was 3’ adenylated. Adaptors were ligated to the ends of these 3’ adenylated cDNA fragments. The ligation products were purified and many rounds of PCR amplification were performed to enrich the purified cDNA template using PCR primer, splint oligo and DNA ligase, followed by sequencing on BGISEQ-500 platform, generating an average 20M reads of 50bp per sample

treatment_protocol: Seeds were manually extracted from the equatorial section of the fruit. The embryo and endosperm were hand-dissected, immediately frozen in liquid nitrogen and stored at -80°C prior to RNA isolation.

value: Moneymaker

tag: age (daf)

tag: temperature

value: High temperature

tag: light

value: High light

value: endosperm


title: HT_HL_End_2


title: PV_2wpi_ctrl_2

description: GeneCounts.txt

value: PV


title: HT_HL_End_1


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C10_ad002

tag: brain region

value: ACA,PL,ILA

value: male


title: PV_2wpi_ctrl_1

description: GeneCounts.txt

value: F

value: CGAT


title: DKMR04_I_CD6KO_unstim_rep1

description:
Sample 4
SAMP1_to_SAMP36_featureCounts_table

value: untreated


title: HT_DL_End_3

source_name: High temperature_Dim light_endosperm

value: Dim light


title: DKMR03_I_CD5KO_unstim_rep1

description:
Sample 3
SAMP1_to_SAMP36_featureCounts_table


title: PV_24wpi_PrD_3

description: GeneCounts.txt

value: prions

value: M


title: HT_DL_End_2


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C10_ad001

tag: Sex


title: DKMR06_I_dKO_4hr_rep1

description:
Sample 6
SAMP1_to_SAMP36_featureCounts_table

value: lymph nodes and spleens


title: DKMR02_I_dKO_unstim_rep1

description:
Sample 2
SAMP1_to_SAMP36_featureCounts_table

value: CD4+ T cells from lymph nodes and spleens


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C1_ad012

tag: strain


title: PV_24wpi_PrD_2

description: GeneCounts.txt

value: GCTA


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C1_ad010

tag: age


title: HT_DL_End_1


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C10_ad004


title: PV_24wpi_ctrl_3

description: GeneCounts.txt

tag: treatment

value: non-infectious brain homogenate

value: AGCT


title: HT_HL_Em_3

source_name: High temperature_High light_embryo

value: Moneymaker

value: High temperature

value: embryo


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C1_ad006


title: HT_HL_Em_2

value: High light


title: PV_24wpi_ctrl_2

description: GeneCounts.txt

value: PV

value: GATC


title: PV_24wpi_PrD_1

description: GeneCounts.txt

value: F

value: ATCG


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C1_ad008

value: ACA,PL,ILA

value: male


title: HT_HL_Em_1


title: CEMBA190117_6A_1_CEMBA190117_6A_2_B9_ad012


title: HT_DL_Em_3

source_name: High temperature_Dim light_embryo

value: Dim light


title: HT_DL_Em_4


title: PV_16wpi_PrD_2

description: GeneCounts.txt

value: prions

value: CGAT


title: PV_16wpi_PrD_3

description: GeneCounts.txt

value: TAGC


title: HT_DL_Em_2


title: PV_16wpi_PrD_1

description: GeneCounts.txt

value: M


title: DKMR01_I_WT_unstim_rep1

description:
Sample 1
SAMP1_to_SAMP36_featureCounts_table

tag: background strain

value: wild-type

value: untreated

value: lymph nodes and spleens

value: CD4+ T cells from lymph nodes and spleens


title: CEMBA190117_6A_1_CEMBA190117_6A_2_B9_ad007


title: HT_DL_Em_1

value: Moneymaker

value: High temperature

tag: tissue

value: embryo


title: CEMBA190117_6A_1_CEMBA190117_6A_2_B9_ad010


title: ST_HL_End_3

value: Standard temperature

value: High light

value: endosperm


title: CEMBA190117_6A_1_CEMBA190117_6A_2_C1_ad002

value: ACA,PL,ILA


title: PV_16wpi_ctrl_3

description: GeneCounts.txt

value: PV

value: non-infectious brain homogenate

value: F

value: TACG


title: CEMBA190117_6A_1_CEMBA190117_6A_2_B9_ad006

value: male


title: ST_HL_End_2


title: CEMBA190117_6A_1_CEMBA190117_6A_2_B9_ad004


title: PV_16wpi_ctrl_1

description: GeneCounts.txt

value: AGCT


title: CEMBA190117_6A_1_CEMBA190117_6A_2_B9_ad002


title: PV_16wpi_ctrl_2

description: GeneCounts.txt

value: M

value: TCGA


title: ST_DL_End_3

value: Dim light


title: noCre_hippocampus_PrD_14

description: GeneCounts.txt

source_name: hippocampus


title: ST_HL_End_1


title: PV_24wpi_ctrl_1

description: GeneCounts.txt

value: CTAG


title: CEMBA190117_6A_1_CEMBA190117_6A_2_B9_ad001


title: CEMBA190117_6A_1_CEMBA190117_6A_2_B9_ad008