title: peripheral blood buffy coat, S1054


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E4_ad002

status: Public on Sep 16 2020

type: SRA

city: La Jolla

first: Joseph

middle: R

last: Ecker

state: CA

address: 10010 North Torrey Pines Road

department: Genomic Analysis Laboratory

country: USA

institute: HHMI-Salk-Institute

library_source: genomic

data_processing:
Library strategy: snmC-seq2
Reads trimed with Cutadapt for adapters and low quality bases (Phred score >= 20)
The resulting files are mapped to in-silico bisulfite converted mm10 reference genome using Bismark.
The raw BAM file will be filtered using samtools to filter reads by MAPQ > 10 and use Picard to remove PCR duplicates
Tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls will be generated with in-house developing package using the filtered BAM files
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls. Columns are: 1. Chromosome, 2. Position, 3. Strand, 4. Cytosin Context, 5. methylated base call, 6. total mapped base call, 7. not used, place holder column

molecule: genomic DNA

organism: Mus musculus

source_name: C57BL/6J Male P56 Mouse Brain

growth_protocol: All mice are C57BL/6J Male, brain dissected in P56

extract_protocol:
the brain of P56 male C57BL/6J mouse will be removed and cut into 600 microns thick coronal sections using a tissue matrix. Then each region will be microdissected from the coronal tissue sections based on identified landmarks and brain atlas coordinates, as defined in Allen brain atlas CCF3 (Avaliable at: http://atlas.brain-map.org). For each dissection region, the tissue from several littermate mice will be combined together into to separate replicate groups. The number of animals in a replicate depends on the size of the tissue. Nuclei preparation will be stained with the fluorescently labeled anti-NeuN body and nucleic acid stain Hoechst 33342.
Library prepared following protocol discribed in Luo, Chongyuan, Angeline Rivkin, Jingtian Zhou, Justin P. Sandoval, Laurie Kurihara, Jacinta Lucero, Rosa Castanon, et al. 2018. “Robust Single-Cell DNA Methylome Profiling with snmC-seq2.” Nature Communications 9 (1): 3824.

tag: brain region

value: DG

tag: Sex

value: male

tag: age

tag: strain


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E3_ad007


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E3_ad012


title: peripheral blood buffy coat, S1055

type: genomic

city: Singapore

first: Jonathan

middle: Yinhao

last: Huang

state: « choose one »

address: 30 Medical Drive

country: Singapore

institute: Singapore Institute for Clinical Sciences

hyb_protocol: Standard Illumina Protocol

scan_protocol: Standard Illumina Protocol

data_processing:
GenomeStudio; R/Rstudio
green (RAW_ID_Grn.idat) and red (RAW_ID_Red.idat) channels provided
[value definition] chip position, DNA extraction, and bisulfite conversion batch, and celltype-adjusted residuals provided

label: Cy3, Cy5

organism: Homo sapiens

source_name: peripheral blood buffy coat

label_protocol: Standard Illumina Protocol

extract_protocol: QIASymphony SP was used for automated purification of (buffy coat/cord tissue) DNA in combination with QIASymphony DSP DNA Midi Kit (Qiagen #937255) and DNA was bisulfite converted using the EZ DNA Methylation Kit™ (Zymo Research).

tag: tissue

value: peripheral blood buffy coat

tag: art treatment status (0 = spontaneous; 1 = assisted reproduction)

tag: maternal age at delivery (years)

tag: maternal ethnicity (0 = chinese; 1 = indian; 2 = malay)

tag: child sex (male = 0; female = 1)

tag: pre-pregnancy bmi (kg/m^2)


title: peripheral blood buffy coat, S1056


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E4_ad012


title: peripheral blood buffy coat, S1052


title: peripheral blood buffy coat, S1050


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E2_ad010


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E2_ad012


title: peripheral blood buffy coat, S1053


title: peripheral blood buffy coat, S1049


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E3_ad002


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E2_ad006


title: peripheral blood buffy coat, S1059


title: peripheral blood buffy coat, S1061


title: peripheral blood buffy coat, S1060


title: peripheral blood buffy coat, S1047


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E2_ad004


title: peripheral blood buffy coat, S1042


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E12_ad012


title: peripheral blood buffy coat, S1044


title: peripheral blood buffy coat, S1043


title: peripheral blood buffy coat, S1046


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E12_ad010


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E12_ad008


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E12_ad007


title: peripheral blood buffy coat, S1080


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E2_ad001


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E12_ad006


title: peripheral blood buffy coat, S1038


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E12_ad002


title: peripheral blood buffy coat, S1040


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E12_ad001


title: peripheral blood buffy coat, S1048


title: peripheral blood buffy coat, S1035


title: peripheral blood buffy coat, S1033


title: peripheral blood buffy coat, S1041


title: peripheral blood buffy coat, S1037


title: peripheral blood buffy coat, S1036


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E12_ad004


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E11_ad012


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E11_ad010


title: peripheral blood buffy coat, S1032


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E11_ad008


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E2_ad002


title: peripheral blood buffy coat, S1031


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E11_ad007


title: peripheral blood buffy coat, S1029


title: peripheral blood buffy coat, S1028


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E11_ad006


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E11_ad002


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E11_ad001


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E10_ad010


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E10_ad012


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E10_ad007


title: peripheral blood buffy coat, S1018


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E10_ad008


title: peripheral blood buffy coat, S1022


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E10_ad001


title: peripheral blood buffy coat, S1023


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E10_ad004


title: peripheral blood buffy coat, S1020


title: peripheral blood buffy coat, S1013


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E10_ad006


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E1_ad007


title: peripheral blood buffy coat, S1016


title: peripheral blood buffy coat, S1017


title: peripheral blood buffy coat, S1015


title: peripheral blood buffy coat, S1027


title: peripheral blood buffy coat, S1011


title: peripheral blood buffy coat, S1010


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E1_ad006


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E1_ad002


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D9_ad010


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D9_ad012


title: peripheral blood buffy coat, S1024


title: peripheral blood buffy coat, S1021


title: peripheral blood buffy coat, S1008


title: peripheral blood buffy coat, S1007


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D9_ad008


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D9_ad007


title: peripheral blood buffy coat, S1003


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D9_ad006


title: peripheral blood buffy coat, S1005


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E1_ad012


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D9_ad002


title: peripheral blood buffy coat, S1002


title: peripheral blood buffy coat, S1012


title: peripheral blood buffy coat, S1000


title: CEMBA190625_10F_3_CEMBA190625_10F_4_E1_ad001


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D9_ad001


title: peripheral blood buffy coat, S0999


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D8_ad010


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D8_ad012


title: peripheral blood buffy coat, S0998


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D8_ad008


title: peripheral blood buffy coat, S0995


title: peripheral blood buffy coat, S0996


title: peripheral blood buffy coat, S1009


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D8_ad006


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D8_ad007


title: peripheral blood buffy coat, S0994


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D8_ad004


title: peripheral blood buffy coat, S0993


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D9_ad004


title: peripheral blood buffy coat, S0992


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D8_ad001


title: peripheral blood buffy coat, S0989


title: peripheral blood buffy coat, S0991


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D8_ad002


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D7_ad008


title: peripheral blood buffy coat, S0987


title: peripheral blood buffy coat, S1001


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D7_ad012


title: peripheral blood buffy coat, S0985


title: peripheral blood buffy coat, S0988


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D7_ad004


title: peripheral blood buffy coat, S0983


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D7_ad006


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D7_ad002


title: peripheral blood buffy coat, S0984


title: peripheral blood buffy coat, S0982


title: peripheral blood buffy coat, S0980


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D7_ad007


title: peripheral blood buffy coat, S0979


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D6_ad010


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D6_ad007


title: peripheral blood buffy coat, S0977


title: peripheral blood buffy coat, S0978


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D6_ad004


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D6_ad002


title: peripheral blood buffy coat, S0974


title: peripheral blood buffy coat, S0973


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D5_ad012


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D5_ad008


title: peripheral blood buffy coat, S0971


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D5_ad007


title: peripheral blood buffy coat, S0969


title: peripheral blood buffy coat, S0972


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D5_ad004


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D5_ad006


title: peripheral blood buffy coat, S0966


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D7_ad001


title: peripheral blood buffy coat, S0965


title: peripheral blood buffy coat, S0963


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D6_ad012


title: peripheral blood buffy coat, S0964


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D5_ad001


title: peripheral blood buffy coat, S0962


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D4_ad010


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D4_ad006


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D4_ad008


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D4_ad007


title: peripheral blood buffy coat, S0961


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D4_ad004


title: peripheral blood buffy coat, S0959


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D3_ad012


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D3_ad010


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D4_ad001


title: peripheral blood buffy coat, S0958


title: peripheral blood buffy coat, S0967


title: peripheral blood buffy coat, S0956


title: peripheral blood buffy coat, S0954


title: peripheral blood buffy coat, S0955


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D3_ad007


title: peripheral blood buffy coat, S0953


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D3_ad006


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D3_ad002


title: peripheral blood buffy coat, S0951


title: peripheral blood buffy coat, S0952


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D2_ad012


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D3_ad001


title: peripheral blood buffy coat, S0950


title: peripheral blood buffy coat, S0949


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D2_ad010


title: peripheral blood buffy coat, S0947


title: peripheral blood buffy coat, S0960


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D2_ad004


title: peripheral blood buffy coat, S0946


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D2_ad002


title: peripheral blood buffy coat, S0945


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D2_ad008


title: peripheral blood buffy coat, S0942


title: peripheral blood buffy coat, S0943


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D12_ad010


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D2_ad001


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D12_ad012


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D3_ad008


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D3_ad004


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D12_ad004


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D12_ad006


title: peripheral blood buffy coat, S0940


title: peripheral blood buffy coat, S0941


title: peripheral blood buffy coat, S0939


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D12_ad008


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D12_ad007


title: peripheral blood buffy coat, S0937


title: peripheral blood buffy coat, S0938


title: peripheral blood buffy coat, S0935


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D11_ad007


title: peripheral blood buffy coat, S0936


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D11_ad008


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D11_ad012


title: peripheral blood buffy coat, S0933


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D12_ad001


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D11_ad010


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D11_ad006


title: peripheral blood buffy coat, S0944


title: peripheral blood buffy coat, S0932


title: peripheral blood buffy coat, S0931


title: peripheral blood buffy coat, S0934


title: peripheral blood buffy coat, S0929


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D10_ad010


title: peripheral blood buffy coat, S0930


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D11_ad001


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D11_ad002


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D10_ad012


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D10_ad008


title: peripheral blood buffy coat, S0927


title: peripheral blood buffy coat, S0926


title: peripheral blood buffy coat, S0928


title: peripheral blood buffy coat, S0923


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D10_ad004


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D10_ad002


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D10_ad006


title: peripheral blood buffy coat, S0924


title: CEMBA190625_10F_3_CEMBA190625_10F_4_D10_ad001


title: peripheral blood buffy coat, S0918


title: peripheral blood buffy coat, S0917


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C9_ad010


title: peripheral blood buffy coat, S0919


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C9_ad012


title: peripheral blood buffy coat, S0916


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C9_ad006


title: peripheral blood buffy coat, S0914


title: peripheral blood buffy coat, S0913


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C9_ad004


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C8_ad012


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C8_ad010


title: peripheral blood buffy coat, S0915


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C8_ad008


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C8_ad007


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C9_ad002


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C8_ad006


title: peripheral blood buffy coat, S0911


title: peripheral blood buffy coat, S0910


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C8_ad002


title: peripheral blood buffy coat, S0921


title: peripheral blood buffy coat, S0907


title: peripheral blood buffy coat, S0904


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C7_ad012


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C7_ad010


title: peripheral blood buffy coat, S0909


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C8_ad001


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C7_ad008


title: peripheral blood buffy coat, S0900


title: peripheral blood buffy coat, S0903


title: peripheral blood buffy coat, S0902


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C7_ad002


title: peripheral blood buffy coat, S0912


title: peripheral blood buffy coat, S0899


title: peripheral blood buffy coat, S0898


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C7_ad006


title: peripheral blood buffy coat, S0897


title: peripheral blood buffy coat, S0895


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C6_ad007


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C6_ad012


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C7_ad001


title: peripheral blood buffy coat, S0896


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C6_ad004


title: peripheral blood buffy coat, S0908


title: peripheral blood buffy coat, A0893


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C6_ad002


title: peripheral blood buffy coat, A0891


title: peripheral blood buffy coat, A0888


title: peripheral blood buffy coat, A0892


title: peripheral blood buffy coat, A0894


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C5_ad010


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C5_ad012


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C5_ad008


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C6_ad001


title: peripheral blood buffy coat, A0886


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C5_ad006


title: peripheral blood buffy coat, A0885


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C6_ad010


title: peripheral blood buffy coat, A0884


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C6_ad008


title: peripheral blood buffy coat, A0887


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C6_ad006


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C5_ad002


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C4_ad012


title: peripheral blood buffy coat, A0882


title: peripheral blood buffy coat, A0883


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C4_ad008


title: peripheral blood buffy coat, A0881


title: peripheral blood buffy coat, A0880


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C4_ad007


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C4_ad010


title: peripheral blood buffy coat, A0879


title: peripheral blood buffy coat, A0890


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C4_ad006


title: peripheral blood buffy coat, A0877


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C4_ad004


title: peripheral blood buffy coat, A0876


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C3_ad012


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C4_ad002


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C5_ad007


title: peripheral blood buffy coat, A0871


title: peripheral blood buffy coat, A0872


title: peripheral blood buffy coat, A0875


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C3_ad010


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C5_ad004


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C3_ad007


title: peripheral blood buffy coat, A0869


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C3_ad004


title: peripheral blood buffy coat, A0865


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C3_ad006


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C3_ad002


title: peripheral blood buffy coat, A0867


title: peripheral blood buffy coat, A0864


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C3_ad001


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C2_ad012


title: peripheral blood buffy coat, A0863


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C2_ad007


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C2_ad008


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C2_ad010


title: peripheral blood buffy coat, A0860


title: peripheral blood buffy coat, A0859


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C2_ad001


title: peripheral blood buffy coat, A0857


title: peripheral blood buffy coat, A0858


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C12_ad010


title: peripheral blood buffy coat, A0856


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C12_ad012


title: peripheral blood buffy coat, A0855


title: peripheral blood buffy coat, A0854


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C12_ad007


title: peripheral blood buffy coat, A0868


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C12_ad006


title: peripheral blood buffy coat, A0852


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C12_ad001


title: peripheral blood buffy coat, A0853


title: peripheral blood buffy coat, A0851


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C11_ad010


title: peripheral blood buffy coat, A0862


title: peripheral blood buffy coat, A0847


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C11_ad007


title: peripheral blood buffy coat, A0861


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C11_ad006


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C2_ad006


title: peripheral blood buffy coat, A0845


title: peripheral blood buffy coat, A0846


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C11_ad004


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C11_ad002


title: peripheral blood buffy coat, A0844


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C12_ad008


title: peripheral blood buffy coat, A0843


title: peripheral blood buffy coat, A0842


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C11_ad001


title: peripheral blood buffy coat, A0841


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C10_ad012


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C10_ad008


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C10_ad006


title: peripheral blood buffy coat, A0839


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C10_ad001


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C10_ad002


title: peripheral blood buffy coat, A0840


title: peripheral blood buffy coat, A0837


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C1_ad012


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C11_ad008


title: peripheral blood buffy coat, A0848


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C1_ad010


title: peripheral blood buffy coat, A0832


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C1_ad008


title: peripheral blood buffy coat, A0833


title: peripheral blood buffy coat, A0831


title: peripheral blood buffy coat, A0830


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C1_ad006


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C1_ad002


title: peripheral blood buffy coat, A0829


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C1_ad004


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C1_ad001


title: peripheral blood buffy coat, A0828


title: peripheral blood buffy coat, A0827


title: peripheral blood buffy coat, A0826


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B9_ad010


title: peripheral blood buffy coat, A0825


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B9_ad008


title: peripheral blood buffy coat, A0838


title: peripheral blood buffy coat, S0818


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B9_ad004


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B9_ad007


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B9_ad001


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B9_ad002


title: peripheral blood buffy coat, A0824


title: peripheral blood buffy coat, A0822


title: CEMBA190625_10F_3_CEMBA190625_10F_4_C1_ad007


title: peripheral blood buffy coat, S0817


title: peripheral blood buffy coat, S0815


title: peripheral blood buffy coat, S0816


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B8_ad012


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B8_ad007


title: peripheral blood buffy coat, S0814


title: peripheral blood buffy coat, S0812


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B8_ad006


title: peripheral blood buffy coat, S0813


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B8_ad004


title: peripheral blood buffy coat, S0821


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B8_ad002


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B8_ad001


title: peripheral blood buffy coat, S0809


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B7_ad012


title: peripheral blood buffy coat, S0807


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B7_ad008


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B9_ad006


title: peripheral blood buffy coat, S0806


title: peripheral blood buffy coat, S0804


title: peripheral blood buffy coat, S0803


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B7_ad007


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B7_ad004


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B7_ad002


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B8_ad008


title: peripheral blood buffy coat, S0799


title: peripheral blood buffy coat, S0802


title: peripheral blood buffy coat, S0800


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B6_ad012


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B6_ad010


title: peripheral blood buffy coat, S0798


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B6_ad008


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B7_ad001


title: peripheral blood buffy coat, S0811


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B6_ad006


title: peripheral blood buffy coat, S0796


title: peripheral blood buffy coat, S0795


title: peripheral blood buffy coat, S0794


title: peripheral blood buffy coat, S0808


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B6_ad002


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B6_ad007


title: peripheral blood buffy coat, S0792


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B7_ad010


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B5_ad012


title: peripheral blood buffy coat, S0791


title: peripheral blood buffy coat, S0790


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B5_ad010


title: peripheral blood buffy coat, S0789


title: peripheral blood buffy coat, S0788


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B5_ad008


title: peripheral blood buffy coat, S0787


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B5_ad006


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B5_ad007


title: peripheral blood buffy coat, S0786


title: peripheral blood buffy coat, S0784


title: peripheral blood buffy coat, S0782


title: peripheral blood buffy coat, S0785


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B5_ad002


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B5_ad001


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B4_ad012


title: peripheral blood buffy coat, S0781


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B4_ad010


title: peripheral blood buffy coat, S0778


title: peripheral blood buffy coat, S0780


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B4_ad008


title: peripheral blood buffy coat, S0777


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B4_ad006


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B4_ad004


title: peripheral blood buffy coat, S0776


title: peripheral blood buffy coat, S0774


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B4_ad001


title: peripheral blood buffy coat, S0773


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B3_ad012


title: peripheral blood buffy coat, S0772


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B3_ad010


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B3_ad008


title: peripheral blood buffy coat, S0771


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B5_ad004


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B3_ad007


title: peripheral blood buffy coat, S0770


title: peripheral blood buffy coat, S0769


title: peripheral blood buffy coat, S0767


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B3_ad002


title: peripheral blood buffy coat, S0766


title: peripheral blood buffy coat, S0768


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B3_ad004


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B2_ad012


title: peripheral blood buffy coat, S0763


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B3_ad001


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B2_ad008


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B4_ad002


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B2_ad007


title: peripheral blood buffy coat, S0765


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B2_ad010


title: peripheral blood buffy coat, S0762


title: peripheral blood buffy coat, S0758


title: peripheral blood buffy coat, S0757


title: peripheral blood buffy coat, S0756


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B12_ad012


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B2_ad001


title: peripheral blood buffy coat, S0755


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B3_ad006


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B12_ad006


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B12_ad004


title: peripheral blood buffy coat, S0752


title: peripheral blood buffy coat, S0753


title: peripheral blood buffy coat, S0754


title: peripheral blood buffy coat, S0749


title: peripheral blood buffy coat, S0751


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B11_ad012


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B11_ad010


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B12_ad002


title: peripheral blood buffy coat, S0760


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B11_ad007


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B2_ad002


title: peripheral blood buffy coat, S0747


title: peripheral blood buffy coat, S0748


title: peripheral blood buffy coat, S0742


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B11_ad001


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B10_ad010


title: peripheral blood buffy coat, S0745


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B11_ad004


title: peripheral blood buffy coat, S0739


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B10_ad006


title: peripheral blood buffy coat, S0743


title: peripheral blood buffy coat, S0737


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B11_ad002


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B10_ad008


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B12_ad007


title: peripheral blood buffy coat, S0735


title: peripheral blood buffy coat, S0733


title: peripheral blood buffy coat, S0732


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B10_ad002


title: peripheral blood buffy coat, S0738


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B10_ad001


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B10_ad004


title: peripheral blood buffy coat, S0731


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B1_ad012


title: peripheral blood buffy coat, S0729


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B1_ad010


title: peripheral blood buffy coat, S0728


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B1_ad008


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B11_ad006


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B1_ad007


title: peripheral blood buffy coat, S0724


title: peripheral blood buffy coat, S0726


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B1_ad006


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B1_ad004


title: peripheral blood buffy coat, S0723


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B1_ad002


title: CEMBA190625_10F_3_CEMBA190625_10F_4_B1_ad001


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A9_ad010


title: peripheral blood buffy coat, S0721


title: peripheral blood buffy coat, S0722


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A9_ad008


title: peripheral blood buffy coat, S0715


title: peripheral blood buffy coat, S0720


title: peripheral blood buffy coat, S0719


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A9_ad012


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A9_ad006


title: peripheral blood buffy coat, S0713


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A8_ad008


title: peripheral blood buffy coat, S0709


title: peripheral blood buffy coat, S0711


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A8_ad012


title: peripheral blood buffy coat, S0708


title: peripheral blood buffy coat, S0712


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A8_ad004


title: peripheral blood buffy coat, S0707


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A8_ad007


title: peripheral blood buffy coat, S0705


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A8_ad001


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A7_ad012


title: peripheral blood buffy coat, S0704


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A7_ad008


title: peripheral blood buffy coat, S0702


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A7_ad010


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A7_ad007


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A7_ad006


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A9_ad007


title: peripheral blood buffy coat, S0700


title: peripheral blood buffy coat, S0698


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A7_ad004


title: peripheral blood buffy coat, S0714


title: peripheral blood buffy coat, S0699


title: peripheral blood buffy coat, S0697


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A7_ad002


title: peripheral blood buffy coat, S0696


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A7_ad001


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A6_ad012


title: peripheral blood buffy coat, S0695


title: peripheral blood buffy coat, S0694


title: peripheral blood buffy coat, S0692


title: peripheral blood buffy coat, S0691


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A6_ad006


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A6_ad007


title: peripheral blood buffy coat, S0688


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A6_ad004


title: peripheral blood buffy coat, S0690


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A6_ad010


title: peripheral blood buffy coat, S0686


title: peripheral blood buffy coat, S0701


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A5_ad012


title: peripheral blood buffy coat, S0684


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A5_ad010


title: peripheral blood buffy coat, S0683


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A5_ad008


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A5_ad007


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A5_ad004


title: peripheral blood buffy coat, S0680


title: peripheral blood buffy coat, S0679


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A5_ad006


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A6_ad008


title: peripheral blood buffy coat, S0681


title: peripheral blood buffy coat, S0677


title: peripheral blood buffy coat, S0678


title: peripheral blood buffy coat, S0675


title: peripheral blood buffy coat, S0674


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A4_ad008


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A4_ad006


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A5_ad002


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A6_ad002


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A4_ad002


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A6_ad001


title: peripheral blood buffy coat, S0672


title: peripheral blood buffy coat, S0671


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A4_ad004


title: peripheral blood buffy coat, S0669


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A4_ad001


title: peripheral blood buffy coat, S0668


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A3_ad012


title: peripheral blood buffy coat, S0682


title: peripheral blood buffy coat, S0667


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A3_ad010


title: peripheral blood buffy coat, S0665


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A3_ad008


title: peripheral blood buffy coat, S0666


title: peripheral blood buffy coat, S0664


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A3_ad004


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A3_ad007


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A3_ad002


title: peripheral blood buffy coat, S0661


title: peripheral blood buffy coat, S0659


title: peripheral blood buffy coat, S0657


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A4_ad012


title: peripheral blood buffy coat, S0662


title: peripheral blood buffy coat, S0656


title: peripheral blood buffy coat, S0655


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A4_ad010


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A2_ad007


title: peripheral blood buffy coat, S0652


title: peripheral blood buffy coat, S0660


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A2_ad001


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A2_ad002


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A2_ad010


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A3_ad001


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A12_ad010


title: peripheral blood buffy coat, S0651


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A2_ad004


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A12_ad008


title: peripheral blood buffy coat, S0648


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A12_ad007


title: peripheral blood buffy coat, S0647


title: peripheral blood buffy coat, S0646


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A2_ad012


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A12_ad006


title: peripheral blood buffy coat, S0645


title: peripheral blood buffy coat, S0644


title: peripheral blood buffy coat, S0643


title: peripheral blood buffy coat, S0642


title: peripheral blood buffy coat, S0653


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A11_ad012


title: peripheral blood buffy coat, S0640


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A11_ad010


title: peripheral blood buffy coat, S0638


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A12_ad012


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A11_ad008


title: peripheral blood buffy coat, S0636


title: peripheral blood buffy coat, S0637


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A11_ad007


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A11_ad006


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A11_ad002


title: peripheral blood buffy coat, S0649


title: peripheral blood buffy coat, S0635


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A11_ad001


title: peripheral blood buffy coat, S0634


title: peripheral blood buffy coat, S0632


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A12_ad004


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A10_ad008


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A10_ad010


title: peripheral blood buffy coat, S0631


title: peripheral blood buffy coat, S0630


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A12_ad001


title: peripheral blood buffy coat, S0628


title: peripheral blood buffy coat, S0629


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A10_ad004


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A10_ad006


title: peripheral blood buffy coat, S0624


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A10_ad007


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A10_ad002


title: peripheral blood buffy coat, S0626


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A1_ad012


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A11_ad004


title: peripheral blood buffy coat, S0623


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A1_ad010


title: peripheral blood buffy coat, S0621


title: peripheral blood buffy coat, S0620


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A1_ad007


title: peripheral blood buffy coat, S0622


title: peripheral blood buffy coat, S0619


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A1_ad006


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A1_ad004


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A1_ad002


title: peripheral blood buffy coat, S0618


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A10_ad012


title: CEMBA190625_10F_3_CEMBA190625_10F_4_A1_ad001


title: peripheral blood buffy coat, S0617


title: peripheral blood buffy coat, S0615


title: peripheral blood buffy coat, S0616


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H9_ad008


title: peripheral blood buffy coat, S0614


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H9_ad012


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H9_ad007


title: peripheral blood buffy coat, S0613


title: peripheral blood buffy coat, S0611


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H9_ad004


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H9_ad006


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H9_ad001


title: peripheral blood buffy coat, S0612


title: peripheral blood buffy coat, S0609


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H9_ad002


title: peripheral blood buffy coat, S0610


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H8_ad007


title: peripheral blood buffy coat, S0608


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H8_ad006


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H8_ad004


title: peripheral blood buffy coat, S0604


title: peripheral blood buffy coat, S0605


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H8_ad012


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H9_ad010


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H8_ad002


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H8_ad001


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H7_ad012


title: peripheral blood buffy coat, S0601


title: peripheral blood buffy coat, S0603


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H7_ad008


title: peripheral blood buffy coat, S0600


title: peripheral blood buffy coat, S0599


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H8_ad008


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H7_ad004


title: peripheral blood buffy coat, S0607


title: peripheral blood buffy coat, S0595


title: peripheral blood buffy coat, S0594


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H7_ad007


title: peripheral blood buffy coat, S0597


title: peripheral blood buffy coat, S0606


title: peripheral blood buffy coat, S0598


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H7_ad006


title: peripheral blood buffy coat, S0593


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H7_ad001


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H6_ad007


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H6_ad012


title: peripheral blood buffy coat, S0592


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H6_ad010


title: peripheral blood buffy coat, S0602


title: peripheral blood buffy coat, S0591


title: peripheral blood buffy coat, S0590


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H6_ad001


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H6_ad002


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H6_ad004


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H5_ad012


title: peripheral blood buffy coat, S0587


title: peripheral blood buffy coat, S0586


title: peripheral blood buffy coat, S0588


title: peripheral blood buffy coat, S0584


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H5_ad008


title: peripheral blood buffy coat, S0583


title: peripheral blood buffy coat, S0582


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H5_ad006


title: peripheral blood buffy coat, S0577


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H6_ad006


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H4_ad010


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H4_ad007


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H5_ad001


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H4_ad012


title: peripheral blood buffy coat, S0575


title: peripheral blood buffy coat, S0580


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H5_ad007


title: peripheral blood buffy coat, S0574


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H4_ad004


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H4_ad006


title: peripheral blood buffy coat, S0573


title: peripheral blood buffy coat, S0572


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H4_ad002


title: peripheral blood buffy coat, S0571


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H3_ad012


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H3_ad010


title: peripheral blood buffy coat, S0566


title: peripheral blood buffy coat, S0570


title: peripheral blood buffy coat, S0562


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H4_ad001


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H3_ad008


title: peripheral blood buffy coat, S0565


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H3_ad006


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H3_ad007


title: peripheral blood buffy coat, S0581


title: peripheral blood buffy coat, S0556


title: peripheral blood buffy coat, S0557


title: peripheral blood buffy coat, S0554


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H2_ad012


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H2_ad008


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H3_ad004


title: peripheral blood buffy coat, S0551


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H2_ad006


title: peripheral blood buffy coat, S0548


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H2_ad004


title: peripheral blood buffy coat, S0547


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H2_ad002


title: peripheral blood buffy coat, S0546


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H2_ad001


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H12_ad012


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H12_ad010


title: peripheral blood buffy coat, S0543


title: peripheral blood buffy coat, S0544


title: peripheral blood buffy coat, S0545


title: peripheral blood buffy coat, S0541


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H12_ad002


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H12_ad007


title: peripheral blood buffy coat, S0537


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H11_ad012


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H3_ad002


title: peripheral blood buffy coat, S0553


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H11_ad007


title: peripheral blood buffy coat, S0538


title: peripheral blood buffy coat, S0550


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H12_ad001


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H11_ad006


title: peripheral blood buffy coat, S0535


title: peripheral blood buffy coat, S0549


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H11_ad002


title: peripheral blood buffy coat, S0534


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H11_ad001


title: peripheral blood buffy coat, S0531


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H10_ad010


title: peripheral blood buffy coat, S0530


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H10_ad008


title: peripheral blood buffy coat, S0540


title: peripheral blood buffy coat, S0529


title: peripheral blood buffy coat, S0525


title: peripheral blood buffy coat, S0526


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H10_ad007


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H10_ad006


title: peripheral blood buffy coat, S0524


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H10_ad004


title: peripheral blood buffy coat, S0536


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H10_ad002


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H1_ad010


title: peripheral blood buffy coat, S0516


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H10_ad001


title: peripheral blood buffy coat, S0520


title: peripheral blood buffy coat, S0515


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H1_ad012


title: peripheral blood buffy coat, S0523


title: peripheral blood buffy coat, S0514


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H2_ad007


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H1_ad007


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H1_ad006


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H1_ad008


title: peripheral blood buffy coat, S0510


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H1_ad004


title: peripheral blood buffy coat, S0508


title: peripheral blood buffy coat, S0507


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H1_ad002


title: peripheral blood buffy coat, S0506


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G9_ad012


title: peripheral blood buffy coat, S0505


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G9_ad008


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G9_ad010


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G9_ad007


title: peripheral blood buffy coat, S0521


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G9_ad006


title: peripheral blood buffy coat, S0501


title: peripheral blood buffy coat, S0502


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G9_ad002


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G9_ad004


title: peripheral blood buffy coat, S0499


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G9_ad001


title: peripheral blood buffy coat, S0500


title: peripheral blood buffy coat, S0498


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G8_ad012


title: peripheral blood buffy coat, S0496


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G8_ad008


title: peripheral blood buffy coat, S0493


title: peripheral blood buffy coat, S0495


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G8_ad007


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G8_ad006


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G8_ad010


title: peripheral blood buffy coat, S0491


title: peripheral blood buffy coat, S0487


title: peripheral blood buffy coat, S0503


title: peripheral blood buffy coat, S0504


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G8_ad002


title: peripheral blood buffy coat, S0485


title: peripheral blood buffy coat, S0483


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G8_ad001


title: peripheral blood buffy coat, S0482


title: peripheral blood buffy coat, S0484


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G7_ad012


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G7_ad010


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G7_ad008


title: peripheral blood buffy coat, S0479


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G7_ad007


title: peripheral blood buffy coat, S0473


title: peripheral blood buffy coat, S0475


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G7_ad006


title: peripheral blood buffy coat, S0474


title: peripheral blood buffy coat, S0478


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G7_ad004


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G7_ad002


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G7_ad001


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G6_ad008


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G8_ad004


title: peripheral blood buffy coat, S0470


title: peripheral blood buffy coat, S0468


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G6_ad007


title: peripheral blood buffy coat, S0464


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G6_ad004


title: peripheral blood buffy coat, S0469


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G6_ad006


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G6_ad002


title: peripheral blood buffy coat, S0467


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G6_ad001


title: peripheral blood buffy coat, S0460


title: peripheral blood buffy coat, S0461


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G5_ad007


title: peripheral blood buffy coat, S0457


title: peripheral blood buffy coat, S0456


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G5_ad004


title: peripheral blood buffy coat, S0472


title: peripheral blood buffy coat, S0458


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G5_ad002


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G5_ad006


title: peripheral blood buffy coat, S0455


title: peripheral blood buffy coat, S0452


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G5_ad001


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G4_ad012


title: peripheral blood buffy coat, S0451


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G4_ad010


title: peripheral blood buffy coat, S0454


title: peripheral blood buffy coat, S0450


title: peripheral blood buffy coat, S0449


title: peripheral blood buffy coat, S0463


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G5_ad012


title: peripheral blood buffy coat, S0462


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G4_ad008


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G5_ad010


title: peripheral blood buffy coat, S0447


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G4_ad004


title: peripheral blood buffy coat, S0448


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G4_ad007


title: peripheral blood buffy coat, S0446


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G4_ad002


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G4_ad006


title: peripheral blood buffy coat, S0444


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G4_ad001


title: peripheral blood buffy coat, S0445


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G3_ad012


title: peripheral blood buffy coat, S0441


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G6_ad012


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G3_ad007


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G3_ad010


title: peripheral blood buffy coat, S0440


title: peripheral blood buffy coat, S0438


title: peripheral blood buffy coat, S0435


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G3_ad002


title: peripheral blood buffy coat, S0433


title: peripheral blood buffy coat, S0432


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G2_ad010


title: peripheral blood buffy coat, S0431


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G2_ad007


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G2_ad006


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G2_ad004


title: peripheral blood buffy coat, S0423


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G2_ad002


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G12_ad012


title: peripheral blood buffy coat, S0425


title: peripheral blood buffy coat, S0422


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G12_ad007


title: peripheral blood buffy coat, S0421


title: peripheral blood buffy coat, S0420


title: peripheral blood buffy coat, S0418


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G12_ad001


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G11_ad012


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G12_ad004


title: peripheral blood buffy coat, S0416


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G3_ad001


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G3_ad006


title: peripheral blood buffy coat, S0414


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G11_ad008


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G11_ad010


title: peripheral blood buffy coat, S0430


title: peripheral blood buffy coat, S0410


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G11_ad006


title: peripheral blood buffy coat, S0412


title: peripheral blood buffy coat, S0409


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G11_ad004


title: peripheral blood buffy coat, S0408


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G11_ad002


title: peripheral blood buffy coat, S0403


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G10_ad010


title: peripheral blood buffy coat, S0417


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G12_ad002


title: peripheral blood buffy coat, S0402


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G10_ad006


title: peripheral blood buffy coat, S0401


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G10_ad008


title: peripheral blood buffy coat, S0415


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G10_ad004


title: CEMBA190625_10F_1_CEMBA190625_10F_2_H11_ad004


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G10_ad002


title: peripheral blood buffy coat, S0397


title: peripheral blood buffy coat, S0399


title: peripheral blood buffy coat, S0393


title: peripheral blood buffy coat, S0394


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G10_ad001


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G10_ad012


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G1_ad012


title: peripheral blood buffy coat, S0392


title: peripheral blood buffy coat, S0390


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G11_ad001


title: peripheral blood buffy coat, S0407


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G1_ad002


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G1_ad007


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G1_ad010


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G1_ad008


title: peripheral blood buffy coat, S0388


title: peripheral blood buffy coat, S0387


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F9_ad012


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G1_ad001


title: peripheral blood buffy coat, S0391


title: peripheral blood buffy coat, S0389


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F9_ad010


title: peripheral blood buffy coat, S0385


title: peripheral blood buffy coat, S0382


title: peripheral blood buffy coat, S0383


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F9_ad006


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F9_ad007


title: peripheral blood buffy coat, S0384


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F9_ad002


title: peripheral blood buffy coat, S0380


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F8_ad012


title: CEMBA190625_10F_1_CEMBA190625_10F_2_G11_ad007


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F9_ad001


title: peripheral blood buffy coat, S0377


title: peripheral blood buffy coat, S0379


title: peripheral blood buffy coat, S0376


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F8_ad010


title: peripheral blood buffy coat, S0375


title: peripheral blood buffy coat, S0374


title: peripheral blood buffy coat, S0386


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F8_ad007


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F8_ad006


title: peripheral blood buffy coat, S0372


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F8_ad002


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F8_ad004


title: peripheral blood buffy coat, S0381


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F7_ad012


title: peripheral blood buffy coat, S0367


title: peripheral blood buffy coat, S0368


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F7_ad010


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F7_ad007


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F7_ad004


title: peripheral blood buffy coat, S0363


title: peripheral blood buffy coat, S0361


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F7_ad002


title: peripheral blood buffy coat, S0362


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F7_ad006


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F6_ad012


title: peripheral blood buffy coat, S0359


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F9_ad008


title: peripheral blood buffy coat, S0357


title: peripheral blood buffy coat, S0369


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F6_ad010


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F6_ad007


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F8_ad001


title: peripheral blood buffy coat, S0356


title: peripheral blood buffy coat, S0371


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F6_ad006


title: peripheral blood buffy coat, S0354


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F6_ad002


title: peripheral blood buffy coat, S0350


title: peripheral blood buffy coat, S0351


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F5_ad012


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F9_ad004


title: peripheral blood buffy coat, S0347


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F5_ad007


title: peripheral blood buffy coat, S0348


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F5_ad006


title: peripheral blood buffy coat, S0345


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F5_ad004


title: peripheral blood buffy coat, S0344


title: peripheral blood buffy coat, S0346


title: peripheral blood buffy coat, S0343


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F4_ad012


title: peripheral blood buffy coat, S0342


title: peripheral blood buffy coat, S0340


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F5_ad008


title: peripheral blood buffy coat, S0339


title: peripheral blood buffy coat, S0358


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F5_ad001


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F4_ad008


title: peripheral blood buffy coat, S0338


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F4_ad006


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F3_ad012


title: peripheral blood buffy coat, S0337


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F3_ad008


title: peripheral blood buffy coat, S0336


title: peripheral blood buffy coat, S0335


title: peripheral blood buffy coat, S0370


title: peripheral blood buffy coat, S0333


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F3_ad002


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F3_ad001


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F4_ad010


title: peripheral blood buffy coat, S0331


title: peripheral blood buffy coat, S0332


title: peripheral blood buffy coat, S0341


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F2_ad010


title: peripheral blood buffy coat, S0330


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F2_ad008


title: peripheral blood buffy coat, S0327


title: peripheral blood buffy coat, S0328


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F2_ad006


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F2_ad004


title: peripheral blood buffy coat, S0326


title: peripheral blood buffy coat, S0324


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F2_ad002


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F4_ad007


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F8_ad008


title: peripheral blood buffy coat, S0320


title: peripheral blood buffy coat, S0322


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F2_ad012


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F12_ad010


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F3_ad004


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F12_ad006


title: peripheral blood buffy coat, S0319


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F12_ad004


title: peripheral blood buffy coat, S0316


title: peripheral blood buffy coat, S0317


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F12_ad002


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F11_ad012


title: peripheral blood buffy coat, S0315


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F12_ad001


title: peripheral blood buffy coat, S0313


title: peripheral blood buffy coat, S0310


title: peripheral blood buffy coat, S0312


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F12_ad012


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F11_ad007


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F11_ad008


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F11_ad010


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F11_ad006


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F11_ad004


title: peripheral blood buffy coat, S0309


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F11_ad002


title: peripheral blood buffy coat, S0307


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F11_ad001


title: peripheral blood buffy coat, S0306


title: peripheral blood buffy coat, S0305


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F10_ad012


title: peripheral blood buffy coat, S0303


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F10_ad010


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F10_ad008


title: peripheral blood buffy coat, S0302


title: peripheral blood buffy coat, S0301


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F10_ad007


title: peripheral blood buffy coat, S0318


title: peripheral blood buffy coat, S0300


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F10_ad004


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F10_ad006


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F10_ad002


title: peripheral blood buffy coat, S0298


title: peripheral blood buffy coat, S0308


title: peripheral blood buffy coat, S0296


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F1_ad010


title: peripheral blood buffy coat, S0294


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F1_ad012


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F1_ad008


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F10_ad001


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F1_ad006


title: peripheral blood buffy coat, S0295


title: peripheral blood buffy coat, S0293


title: peripheral blood buffy coat, S0292


title: peripheral blood buffy coat, S0288


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F1_ad004


title: peripheral blood buffy coat, S0299


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E9_ad012


title: peripheral blood buffy coat, S0286


title: peripheral blood buffy coat, S0287


title: SIN3A_KD rep3

city: Hangzhou

first: Kun

last: Zhang

address: 866 Yuhangtang Rd

country: China

institute: Zhejiang University

library_source: transcriptomic

data_processing:
Reads were trimmed to remove the bases with low quality and adapter sequences
The trimmed reads were assigned directly to transcripts and counted with Salmon software
Gene expression level was quantified and normolized by Salmon(v0.14.1)
Differentially expressed genes between NC and SIN3A_KD group were selected by DESeq2
Genome_build: Sscrofa11.1
Supplementary_files_format_and_content: Text files include normalized counts by DESeq2 for each sample

molecule: polyA RNA

organism: Sus scrofa

source_name: embryo

extract_protocol:
Total RNA extraction was performed using Picopure RNA isolation kit (Life Technologies, Grand Island, NY, USA) based on the manufacturer's manual. Before RNA isolation, equal amount of GFP and RFP mRNA were added to each group as a spike-in control.
mRNAs separation was achieved using oligo(dT)25 beads. Sequencing libraries were constructed with NEB Next Ultra RNA Library Prep Kit for Illumina (New England Biolabs) according to the instruction. In brief, mRNAs were fragmented followed by reverse transcription. The cDNA library underwent end repair, poly(A)-tailing, adaptor ligation, and PCR amplification for 12–15 cycles to prepare sequencing libraries.

value: embryo

tag: developmental stage

value: 2-cell embryos (32 h after parthenogenetic activation)


title: NC rep3


title: NC rep2


title: SIN3A_KD rep2


title: SIN3A_KD rep1


title: peripheral blood buffy coat, S0297


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E9_ad010


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F6_ad001


title: peripheral blood buffy coat, S0285


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E9_ad008


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E9_ad007


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F1_ad002


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E9_ad004


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E9_ad002


title: SL rep2

city: Hong Kong

first: Tingting

last: Xiao

address: Kadoorie Building, Pokfulam Road, Hong kong Island

institute: University of Hong Kong

data_processing:
First, sequencing adapters were removed from sequencing reads using cutadapt v1.9. Secondly, low quality reads were trimmed by fqtrim v0.95 using a sliding-window algorithm. Thirdly, reads were aligned to the host genome using bowtie2 v2.2.0 to remove host contamination. Once quality-filtered reads were obtained, they were de novo assembled to construct the Metatrascriptome for each sample by Trinityv2.2.0.
All Metatrascriptome contigs of all samples were clustered by CD-HIT v4.6.1 to obtain unigenes. Unigene abundance for a certain sample were estimated by TPM based on the number of aligned reads by bowtie2 v2.2.0. The lowest common ancestor taxonomy of unigenes were obtained by aligning them against the NCBI NR database by DIAMOND v 0.7.12. Similarly, the functional annotation (GO, KEGG, eggnog, CAZy, CARD, PHI) of unigenes were obtained. Based on the taxonomic and functional annotation of unigenes, along with the abundance profile of unigenes, the differential analysis were carried out at each taxonomic or functional or gene-wise level by Fisher's exact test (non-replicated groups) or Kruskal Wallis test (replicated groups).

molecule: total RNA

organism:
Levilactobacillus brevis
Streptococcus thermophilus ASCC 1275

source_name: Fermented milk

extract_protocol:
Total RNA was isolated and purified using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer's procedure. The RNA amount and purity of each sample was quantified using NanoDrop ND-1000 (NanoDrop, Wilmington, DE, USA). The RNA integrity was assessed by Agilent 2100 with RIN number >7.0.
Approximately 5 ug of total RNA was used to deplete ribosomal RNA according to the manuscript of the Ribo-Zero™ rRNA Removal Kit (Illumina, San Diego, USA). After removing ribosomal RNAs, the left RNAs were fragmented into small pieces using divalent cations under high temperature. Then the cleaved RNA fragments were reverse-transcribed to create the cDNA,which were next used to synthesise U-labeled second-stranded DNAs with E. coli DNA polymerase I, RNase H and dUTP.. An A-base is then added to the blunt ends of each strand, preparing them for ligation to the indexed adapters. Each adapter contains a T-base overhang for ligating the adapter to the A-tailed fragmented DNA. Single- or dual-index adapters are ligated to the fragments, and size selection was performed with AMPureXP beads. After the heat-labile UDG enzyme treatment of the U-labeled second-stranded DNAs,The ligated products are amplified with PCR by the following conditions: initial denaturation at 95℃ for 3 min; 8cycles of denaturation at 98℃ for 15 sec, annealing at 60℃ for 15 sec, and extension at 72℃ for 30 sec; and then final extension at 72℃ for 5 min. The average insert size for the final cDNA library was 300 bp (±50 bp).

tag: fermentation time

tag: treatment

value: lactic acid bacteria


title: S rep2

organism: Streptococcus thermophilus ASCC 1275


title: SL rep3


title: S rep3


title: S rep1


title: peripheral blood buffy coat, S0281


title: SL rep1


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F1_ad007


title: peripheral blood buffy coat, S0279


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E9_ad001


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E8_ad012


title: HCT116-WT-1

city: Ann Arbor

first: Armand

last: Bankhead

state: MI

address: 1415 Washington Heights

department: Biostatistics

institute: University of Michigan

data_processing:
Reads were mapped to GRCh38 using STAR v2.5.2
Gene quantifications were calculated using Cufflinks v2.2.1 to quantify refGene annotations. Gene read counts calculated using featureCounts v1.6.1.
Genome_build: GRCh38
Supplementary_files_format_and_content: tab-delimited text files include FPKM values and read counts per gene for each Sample

growth_protocol: HCT116 (ATCC, CCL-247) and GSTO1 KO cells were cultured in RPMI 1640 supplemented with 10% fetal bovine serum. U-87 MG (generously provided by Dr. Alan L. Epstein (University of Southern California, Los Angeles, CA)) Cells were kept at 37 ̊C in a humidified atmosphere of 5% CO2. All cell lines were maintained in culture under 30 passages and tested regularly for Mycoplasma contamination using PlasmoTest.

extract_protocol:
Cells were lysed with TRIzol® Reagent (ThermoFisher Scientific, Waltham, MA) at room temperature. RNA was further purified with DirectZol kit (Zymo Research, Irvine, CA).
RNA quality was assessed using the TapeStation (Agilent Technologies, Santa Clara, CA). Samples with RINs (RNA Integrity Numbers) of 8 or greater were prepared with TruSeq Stranded mRNA Library Prep (Illumina) per the supplier’s protocol with 1μg of RNA and 12 cycles of PCR amplification. Libraries were checked for size on the TapeStation and quantified using the Kapa Biosystems library quantification kit (Illumina). The libraries were barcoded, pooled and sequenced using 50bp paired-end 50bp (U-87 MG, University of Michigan DNA Sequencing Core, MI) and single-end 50bp (HCT116, University of Michigan DNA Sequencing Core, MI) sequencing.

treatment_protocol: GSTO1 knockout cancer cell lines were generated using the Edit-R CRISPR-Cas9 system from Dharmacon (GE Healthcare, Lafayette, CO). Edit-R Lentiviral hEF1α-Blast-Cas9 lentiviral particles (generously provided by Phillip L. Palmbos, University of Michigan, MI) were transduced into U-87 MG, HCT116 and A172 cells and stable Cas9 expressing lines were generated by blasticidin selection. Cells were co-transfected with tracrRNA and GSTO1-targeting crRNA (CM-011950-01-0002) specific for exon 2 or non-targeting crRNA control (U-002000-05). Individual colonies were further selected and screened for loss of GSTO1 expression. Targeted mutations were confirmed using Sanger sequencing.

tag: cell line


title: HCT116-WT-3


title: HCT116-GSTO1_KO-2


title: peripheral blood buffy coat, S0283


title: HCT116-GSTO1_KO-3


title: HCT116-GSTO1_KO-1


title: peripheral blood buffy coat, S0278


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E8_ad010


title: SK-N-AS [ATAC-Seq]

city: Heidelberg

first: Carl

last: Herrmann

address: INF 267

department: Medical Faculty

country: Germany

institute: University Heidelberg

data_processing:
Reads were trimmed using the Trimgalore
Genome alignment was done using Bowtie2 with standard parameter
Duplicates were removed as well as reads mapping multiple times
Signal was scaled using the Reads Per Kilobase per Million mapped reads (RPKM) normalization and converted into a bigwig track using the bamCompare tool of the deepTools2 suite
Peaks were called using the callpeak mode in MACS
Genome_build: hg19
Supplementary_files_format_and_content: bigwig files containing ATAC-seq signal intensity
Supplementary_files_format_and_content: macs2 narrowpeak files containing peaks called for every sample

source_name: Neuroblastoma cell lines

growth_protocol: Neuroblastoma cell lines were cultured in RPMI1640 medium at 37°C with 5 % CO2 and supplemented with 1 % penicillin/streptomycin and 10% FCS (fetal calf serum).

extract_protocol:
For cell lysis, cells were resuspended in cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630) as described previously by Buenrostro et al..
Library preparation was performed by using Nextera PCR primer and NEBNext High-Fidelity 2x PCR Master Mix. Samples were amplified for 11 cycles in total according to the manufacturer’s protocol. Libraries were sequenced using a HiSeq 2000 V4 platform with 50bp single read.

value: SK-N-AS [ATAC-Seq]


title: peripheral blood buffy coat, S0282


title: KELLY [ATAC-Seq]

value: KELLY [ATAC-Seq]


title: SK-N-AS [HiChIP]

data_processing:
Library strategy: HiChIP
HiChIP reads were aligned using HiC-pro with the default configuration.
Valid pairs were analyzed using step 4 and 5 of Mango (Phanstiel et al.). An FDR of 0.05 was applied to all samples.
Genome_build: hg19 (GRCh37)
Supplementary_files_format_and_content: Bedpe files containing interacting regions (inferred by Mango). The columns are: chromosome1, start1, end1, chromosome2, start2, end2, PETs supporting the interaction, the adjusted P-value of the interaction.

source_name: Neuroblastoma cell line

growth_protocol: Neuroblastoma cell lines were cultured in RPMI1640 medium at 37°C with 5% CO2 and supplemented with 1% penicillin/streptomycin and 10% FCS (fetal calf serum).

extract_protocol:
For cell lysis, cross-linked samples were incubated in Hi-C lysis buffer (10 mM Tris-HCl (pH 7.5), 10 mM NaCl, 0.2% NP-40 and protease inhibitor) at 4°C for 30 min and rotating as described previously by Mumbach et al. Sonication was performed using the Covaris LE220 using the sonication parameters: Duty cycle of 15%, PIP of 350W and cycle per burst of 200 for 4 minutes in total.
Library preparation was performed using Illumina Nextera primer (Illumina Nextera Index Kit 24 Indexes – 96 samples) and Nextera PCR master mix (Illumina Nextera DNA Library Prep Kit 24 samples) and reaction was performed for 8 cycles in total according to the manufacturer’s protocol.

value: SK-N-AS

tag: cell type

value: Neuroblastoma cell line


title: peripheral blood buffy coat, S0277


title: NC rep1


title: peripheral blood buffy coat, S0280


title: peripheral blood buffy coat, S0366


title: HCT116-WT-2


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E8_ad008


title: CLB-GA [HiChIP]

value: CLB-GA


title: peripheral blood buffy coat, S0275


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E8_ad006


title: peripheral blood buffy coat, S0273


title: peripheral blood buffy coat, S0276


title: peripheral blood buffy coat, S0272


title: NSP054-PT01

data_processing:
Reads were trimmed using the Trimgalore
Genome alignment was done using Bowtie2 with standard parameter
Duplicates were removed as well as reads mapping multiple times
Input and corresponding IP datasets were scaled using the SES method and converted into a bigwig track using the bamCompare tool of the deepTools2 suite
Peaks were called using the callpeak mode in MACS
Genome_build: hg19
Supplementary_files_format_and_content: bigwig files containing H3K27ac signal intensity

source_name: Neuroblastoma primary samples

extract_protocol:
For cell lysis, cross-linked samples were incubated 30 min on ice in 950 µL RIPA I (10 mM Tris-HCl pH 8.0, 1 mM EDTA pH 8.0, 140 mM NaCl, 0.2 % SDS, 0.1 % DOC) supplemented with protease inhibitors. Sonication was performed using Bioruptor Plus sonication device (30-60 min each with 30s on and 30s off intervals). After centrifugation (20.000g, 10min and 4°C), supernatant was used for immunoprecipitation.
Library preparation was performed using the NEBNext Ultra DNA Library Prep Kit (New England Biolabs) according to the manufacturer’s protocol. This includes the steps of end repair, a-base addition, adapter ligation and amplification. Finally, samples were mixed in equal molar ratios and sequenced on an Illumina sequencing platform.

value: Neuroblastoma primary samples

tag: type

value: Tumor

tag: relapse

value: No

tag: gender

value: M

value: NonAmp

tag: Stage


title: NSP052-PT01


title: NSP050-PT01

value: Amp


title: NSP051-PT01


title: NSP056-PT01


title: NSP053-PT01


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E8_ad002

tag: brain region

value: DG

tag: Sex

tag: age


title: NSP049-PT01

tag: tissue

value: F


title: CEMBA190625_10F_1_CEMBA190625_10F_2_F7_ad001

value: male

tag: strain


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E8_ad004


title: NSP048-PT01


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E8_ad001


title: peripheral blood buffy coat, S0271

value: peripheral blood buffy coat

tag: art treatment status (0 = spontaneous; 1 = assisted reproduction)

tag: maternal age at delivery (years)

tag: maternal ethnicity (0 = chinese; 1 = indian; 2 = malay)


title: peripheral blood buffy coat, S0269

tag: child sex (male = 0; female = 1)

tag: pre-pregnancy bmi (kg/m^2)


title: NSP047-PT01


title: NSP046-PT01


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E7_ad012


title: NSP055-PT01


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E8_ad007


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E7_ad008


title: NSP045-PT01


title: peripheral blood buffy coat, S0265


title: NSP044-PT01


title: reprogrammed1

city: Philadelphia

first: Feng

last: Li

state: PA

address: 3501 civic center blvd

institute: Children's hospital of philadelphia

data_processing:
Zebracall version 0.5.0.13875 used for basecalling.
use Bowtie2 (-q --phred64 --sensitive --dpad 0 --gbar99999999 --mp 1,1 --np 1 --score-min L,0,-0.1 -p 16 -k 200) to map clean reads to reference gene and use HISAT (-p 8 --phred64 --sensitive -I 1 -X 1000) to reference genome.
Fragments Per Kilobase of transcript per Million mapped reads (FPKM) method is used in calculated expression level following standard protocol.
Genome_build: Release 6 plus ISO1 MT
Supplementary_files_format_and_content: microsoft offfic .xls   including gene ID, transcript ID and FPKM value for each

organism: Drosophila melanogaster

source_name: the central nervous systems of drosophila larvae

extract_protocol:
The central nervous systems of larvae were dissected out and underwent RNA purification with Quick-RNA miniprep plus kit (Zymo).
Integrity of eluted RNA was analyzed by NanoDrop (Thermo Scientific) and 2100 Agilent Bioanalyzer (Agilent) prior to subsequent experiments. Approximately 1 μg total RNA for each genotype was used. Oligo (dT) magnetic beads were used to select mRNA, then cDNA was synthesized from mRNA fragments by random N6 primers, followed by a second strand cDNA synthesis. After end-repair and adaptor ligation, the double stranded DNA was denatured and circularized on single strand to form the final library.

value: 96 hours after eggs laying

tag: genotype

value: repo-Gal4>Egfr(constutively activated), PI3K92E(constutively activated)


title: NSP043-PT01


title: peripheral blood buffy coat, S0268


title: NSP042-PT01


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E7_ad006


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E7_ad004


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E7_ad007


title: peripheral blood buffy coat, S0263


title: reprogrammed2


title: control1


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E7_ad002


title: control2


title: NSP040-PT01


title: peripheral blood buffy coat, S0262


title: NSP041-PT01


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E7_ad001


title: peripheral blood buffy coat, S0260


title: Splenocyte_HyPR_1000probes_8

city: Stanford

first: Jesse

middle: M

last: Engreitz

address: Biomedical Innovation Building Rm 3700

institute: Stanford University

description: HyPR-seq probes against spleen markers

data_processing:
Library strategy: HyPR-seq
Filter reads with fastp
Extract cell barcodes with UMI-Tools
Map reads to custom index with Bowtie
Convert to bam files with SAMTools
Generate count tables with UMI-Tools
Genome_build: hg19
Supplementary_files_format_and_content: HyPR-seq: count tables (cells by probes or cells by genes) for all cells that passed QC

molecule: other

source_name: Murine Spleen

growth_protocol:
K562: The immortalized myelogenous leukemia K562 cell line was obtained from ATCC (ATCC, CCL-243). Cells were maintained in RPMI 1640 Medium (Corning, 10-040-CM) supplemented with 10% fetal bovine serum (Life Technologies, 16140071) and 1% penicillin-streptomycin (Life Technologies, 15140163). Cell lines were cultured at 37°C with 5% CO₂ and were subcultured twice a week by aspirating off all cell culture medium except 0.5 ml in a T-75 flask and 1 ml in a T-175 flask, and refreshing the flasks with 13 ml and 49 ml of medium respectively.
THP1: The human monocyte THP1 cell line was obtained from ATCC (ATCC, TIB-202). Cells were maintained in RPMI 1640 Medium (Corning, 10-040-CM) supplemented with 10% fetal bovine serum (Life Technologies, 16140071) and 1% penicillin-streptomycin (Life Technologies Inc., 15140163). Cell lines were cultured at 37°C with 5% CO₂ and were subcultured twice a week by aspirating off all cell culture medium except 0.5 ml in a T-75 flask and 1 ml in a T-175 flask, and refreshing the flasks with 13 ml and 49 ml of medium respectively.

extract_protocol:
For HyPR-Seq, cells are harvested in 1X phosphate buffered saline (PBS) at 350g for 5 mins at 4°C in a swinging bucket rotor. Cells were fixed in 4% Formaldehyde solution (4% formaldehyde in 1XPBS and 0.1% Tween 20) for one hour at room temperature at a concentration of 1 million cells per ml. The fixed cells are then washed twice with of 1X PBS containing 0.2% Tween 20 (1X PBST) at 850g for 5 mins at 4°C. The washed cells were then permeabilized in 70% ice cold ethanol at a concentration of 1 million cells per ml and stored at 4°C for 10 mins. The permeabilized cells are then harvested and washed twice with 1XPBST (850g for 5 mins). The cells were then transferred to 2ml V-bottom tubes for subsequent probe hybridization with up to 5 million (cells per hybridization reaction. The cells were resuspended in 0.5mls of probe hybridization buffer (5X SSC, 30% formamide, 0.1% Tween 20) and was incubated at 37°C for 5 min. Probes mix was prepared at a concentration of 20nM per probe in 2mls of probe hybridization buffer. The prehybridized cells are harvested at 850g for 5 mins at room temperature, resuspended in the probes mix and incubated overnight at 37°C in a hybridization oven (VWR Cat# 10055-006). The probe hybridized cells were harvested (850g for 5 mins) and washed with probe hybridization buffer with a 10min incubation of cells in buffer at 37oC before the spin. These wash and incubation steps were done a total of four times. After the last spin, the cells were incubated in a 75nM H1 hairpin mix. To prepare the H1 hairpin mix for one hybridization reaction, 5ul of 3uM H1 hairpin was snap cooled (heat at 95°C for 90 seconds and cooled to room temperature for 30 min) and resuspended in 200ul of 5XSSCT buffer (5XSSC and 0.1% Tween 20). The cells were resuspended in 200ul of H1 hairpin mix was incubated at 37°C for one hour in the hybridization oven. After the H1 hairpin hybridization, the cells are washed twice with 5XSSCT at room temperature (850g for 5 mins). The cells are then resuspended in a 75nM “readout” oligo mix. To prepare the “readout” mix for one hybridization reaction, 5ul of 3uM of “readout” oligo was snap cooled as detailed above and resuspended in 200ul of 5XSSCT buffer. The cells are resuspended in the oligo mix and incubated 37°C for one hour. After oligo incubation, the cells were harvested and washed in 5X SSCT three times (850g for 5 mins). After the last spin the cells were suspended in and washed 1X T4 Ligase reaction buffer (NEB #B0202S). Finally, the cells were resuspended in Ligase buffer (1X T4 ligase buffer, 1:50 dilution of T4 DNA ligase NEB #M0202S) and incubated at room temperature for one hour. Following this final incubation, cells were washed three times in 1XPBST and filtered through a 20micron filter (20um pluriStrainer cat. 43-0020-01). The cells were then counted using a hemocytometer (Fisher Scientific SKU#DHCF015) and checked for single cell suspension before proceeding to droplet generation. For long term storage of the cells, add RNase inhibitor to the cell suspension (NEB M0314S).
To generate emulsions, we first combined 2X Evagreen supermix (Biorad cat 186-4033), 500nM indexing primer (Table S3), 2,000 cells and 20,000 barcoded beads (Chemgenes) in a total of 20ul per reaction. Once the PCR mix is made, emulsions were generated using QX200 Digital Droplet Generator (BioRad #1864002) as per manufacturer’s instructions. Briefly, the droplet generation cartridge (Biorad #186-4007) was inserted into the holder (Biorad 186-3051) and 20ul of the prepared PCR mix was added to the middle sample wells as per instructions. 70ul of the droplet generating oil (Biorad, 186-4005) was added into the bottom row and the gasket (Biorad 186-4007) was placed over the filled cartridge and placed in the droplet generator. Once the droplets were formed, the cartridge containing the emulsions was placed under the UV lamp (6.5 J/cm2 at 365 nm) about 3-8cms away from the bulb for 5min. After UV exposure, ~50ul of droplets per well were transferred to 96 well plates (Eppendorf 951020362) and sealed with foil (Biorad #181-4040) using a plate sealer (PX1 Plate Sealer 181-4000) for droplet PCR amplification (Eppendorf Mastercycler Pro E90030010). The following cycling conditions were used: Denaturation: 94°C for 30 sec; Cycling: 30 cycles of 94°C for 5 sec, 64°C for 30 sec, and 72°C for 30 sec; Final extension: 72°C for 5 min. All PCR cycling steps were performed with 50% ramp rate (2°C/min). To break the emulsions, one to four PCR wells were combined in a tube and equal volumes of 100% PFO were added (~150l). The tubes were vortexed for 5 sec to ensure complete emulsion breakage and spun at 1000g for 1min. The top aqueous layer was carefully removed and cleaned with 1.8X SPRI beads according to manufacturer’s instructions. The amplicon libraries were loaded on a gel to determine size (206bp) and to ensure no primer dimers remained. The libraries were quantified by Qubit before proceeding to sequencing.

treatment_protocol: THP1 cells were stimulated using cell culture medium with LPS at a final concentration of 0.5 µg/ml, diluted from a 5 mg/ml LPS stock (Millipore Sigma, L3024), for 20 hours.

tag: data source

value: primary tissue

value: Spleen

tag: timepoint

tag: stimulation

tag: probe number


title: Splenocyte_HyPR_1000probes_7

description: HyPR-seq probes against spleen markers

tag: cell type


title: NSP039-PT01

tag: type

tag: relapse

tag: gender

tag: Stage


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E6_ad012

tag: brain region

tag: Sex

tag: age


title: NSP038-PT01

tag: tissue


title: peripheral blood buffy coat, S0264

tag: art treatment status (0 = spontaneous; 1 = assisted reproduction)

tag: maternal age at delivery (years)

tag: maternal ethnicity (0 = chinese; 1 = indian; 2 = malay)


title: Splenocyte_HyPR_1000probes_5

description: HyPR-seq probes against spleen markers

tag: genotype


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E6_ad010

tag: strain


title: peripheral blood buffy coat, S0259

tag: child sex (male = 0; female = 1)

tag: pre-pregnancy bmi (kg/m^2)


title: peripheral blood buffy coat, S0258


title: NSP037-PT01


title: Splenocyte_HyPR_1000probes_4

description: HyPR-seq probes against spleen markers

tag: data source

tag: timepoint

tag: stimulation

tag: probe number


title: NSP036-PT01

tag: type

tag: relapse

tag: gender


title: peripheral blood buffy coat, S0261


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E6_ad006

tag: brain region

tag: Sex


title: peripheral blood buffy coat, S0255

tag: tissue


title: Splenocyte_HyPR_1000probes_6

description: HyPR-seq probes against spleen markers

tag: cell type


title: NSP034-PT01

tag: Stage


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E5_ad012

tag: age


title: Splenocyte_HyPR_1000probes_3

description: HyPR-seq probes against spleen markers

tag: genotype


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E6_ad008

tag: strain


title: Splenocyte_HyPR_1000probes_2

description: HyPR-seq probes against spleen markers


title: peripheral blood buffy coat, S0256

tag: art treatment status (0 = spontaneous; 1 = assisted reproduction)

tag: maternal age at delivery (years)

tag: maternal ethnicity (0 = chinese; 1 = indian; 2 = malay)

tag: child sex (male = 0; female = 1)


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E5_ad010


title: peripheral blood buffy coat, S0253

tag: pre-pregnancy bmi (kg/m^2)


title: Splenocyte_HyPR_250probes_7

description: HyPR-seq probes against spleen markers

tag: data source

tag: timepoint

tag: stimulation

tag: probe number


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E5_ad008

tag: brain region


title: peripheral blood buffy coat, S0251


title: NSP032-RT03

tag: type

tag: relapse

value: Yes

tag: gender


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E5_ad007

tag: Sex


title: Splenocyte_HyPR_250probes_6

description: HyPR-seq probes against spleen markers

tag: cell type


title: peripheral blood buffy coat, S0249

tag: tissue


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E5_ad006

tag: age

tag: strain


title: NSP032-RT02

tag: Stage


title: Splenocyte_HyPR_250probes_5

description: HyPR-seq probes against spleen markers

tag: genotype


title: peripheral blood buffy coat, S0257

tag: art treatment status (0 = spontaneous; 1 = assisted reproduction)

tag: maternal age at delivery (years)

tag: maternal ethnicity (0 = chinese; 1 = indian; 2 = malay)

tag: child sex (male = 0; female = 1)


title: NSP032-RT01


title: NSP032-RM01

value: Metastasis


title: NSP035-PT01


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E5_ad004


title: Splenocyte_HyPR_250probes_4

description: HyPR-seq probes against spleen markers

tag: data source

tag: timepoint

tag: stimulation


title: Splenocyte_HyPR_1000probes_1

description: HyPR-seq probes against spleen markers

tag: probe number


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E5_ad001

tag: brain region


title: NSP032-PT01

tag: type

tag: relapse

tag: gender


title: peripheral blood buffy coat, S0244

tag: tissue

tag: pre-pregnancy bmi (kg/m^2)


title: NSP033-PT01


title: peripheral blood buffy coat, S0246


title: Splenocyte_HyPR_250probes_3

description: HyPR-seq probes against spleen markers

tag: cell type


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E4_ad012

tag: Sex

tag: age

tag: strain


title: peripheral blood buffy coat, S0243

tag: art treatment status (0 = spontaneous; 1 = assisted reproduction)

tag: maternal age at delivery (years)


title: NSP031-PT01

tag: Stage


title: Splenocyte_HyPR_250probes_2

description: HyPR-seq probes against spleen markers

tag: data source

tag: genotype


title: NSP030-PT01


title: peripheral blood buffy coat, S0242

tag: maternal ethnicity (0 = chinese; 1 = indian; 2 = malay)

tag: child sex (male = 0; female = 1)


title: Splenocyte_HyPR_250probes_1

description: HyPR-seq probes against spleen markers

tag: timepoint

tag: stimulation

tag: probe number


title: NSP029-PT01

tag: type

tag: relapse

tag: gender


title: Kidney_DKD_2

description: HyPR-seq probes against murine kidney cell type markers

source_name: Murine Kidney

tag: cell type

value: Kidney

value: ob/ob


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E4_ad007

tag: brain region

tag: Sex


title: peripheral blood buffy coat, S0248

tag: tissue

tag: pre-pregnancy bmi (kg/m^2)


title: peripheral blood buffy coat, S0241

tag: art treatment status (0 = spontaneous; 1 = assisted reproduction)

tag: maternal age at delivery (years)


title: peripheral blood buffy coat, S0240


title: Kidney_DKD_1

tag: data source


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E4_ad004

tag: age

tag: strain


title: Kidney_WT_2

tag: genotype

value: wt/wt


title: NSP028-PT01

value: Neuroblastoma primary samples

value: Tumor

value: No

tag: Stage


title: peripheral blood buffy coat, S0239

tag: maternal ethnicity (0 = chinese; 1 = indian; 2 = malay)

tag: child sex (male = 0; female = 1)


title: NSP027-PT01

tag: type

value: NonAmp


title: NSP026-PT01

tag: relapse

value: M


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E4_ad002

value: DG


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E4_ad001

value: male


title: peripheral blood buffy coat, S0238

value: peripheral blood buffy coat


title: Kidney_WT_1

tag: timepoint

tag: stimulation

tag: probe number


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E3_ad012

tag: brain region

tag: Sex


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E4_ad008


title: NSP025-PT01

tag: tissue

tag: gender

value: F


title: NSP024-PT01

value: Amp


title: peripheral blood buffy coat, S0234

tag: art treatment status (0 = spontaneous; 1 = assisted reproduction)

tag: maternal age at delivery (years)

tag: pre-pregnancy bmi (kg/m^2)


title: peripheral blood buffy coat, S0233


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E3_ad007

tag: age

tag: strain


title: NSP022-PT01

tag: Stage


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E3_ad006


title: peripheral blood buffy coat, S0232

tag: maternal ethnicity (0 = chinese; 1 = indian; 2 = malay)


title: peripheral blood buffy coat, S0231

tag: child sex (male = 0; female = 1)


title: NSP021-PT01

tag: type


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E2_ad012


title: peripheral blood buffy coat, S0235


title: peripheral blood buffy coat, S0229


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E2_ad010


title: NSP020-PT01

tag: relapse


title: peripheral blood buffy coat, S0226


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E3_ad010


title: peripheral blood buffy coat, S0225


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E2_ad008


title: NSP018-PT01


title: NSP019-PT01


title: NSP023-PT01


title: Fibroblastic reticular cells 15

type: RNA

city: Rennes

first: Patricia

last: Amé-Thomas

address: 2 Avenue du Pr Léon Bernard

department: Faculté de Médecine

country: France

institute: INSERM UMR1236

description: FRCLs were obtained from TSCs stimulated with lymphotoxin-alpha1béta2 and tumor necrosis factor-alpha

hyb_protocol: Following fragmentation, cARN were hybridized on GeneChip Human U133Plus2 arrays

scan_protocol: GeneChips were scanned using the Genechip Scanner Affymetrix 3000

data_processing: Raw data (.CEL Intensity files) were extracted from the scanned images using the Affymetrix GeneChip® Command Console (AGCC). Normalization was performed by using the RMA algorithm using GC content from the Partek Genomics Suite software (Partek, St Louis, MO).  Dataset was then filtering by eliminating PS with no expression in all dataset and/or no gene symbol annotations.

label: biotin

source_name: In vitro expanded stroma cells

label_protocol: Biotinylated cRNA were amplified according to the small sample labeling protocol (Affymetrix, Santa Clara, CA)

growth_protocol: Stromal cells were obtained as previously described (Amé-Thomas P, Blood 2007).

extract_protocol: RNA extraction was  performed using RNEasy mini kit (Qiagen, Valencia, CA) according to the manufacturer's instructions.

treatment_protocol: Stromal cells were expanded from tonsil mononuclear cells in aphaMEM+10%FBS

tag: status

value: Stimulated

value: Tonsil


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E2_ad007


title: Tonsil stromal cells 15

description: TSCs were expanded from tonsil mononuclear cells in aphaMEM+10%FBS

value: Unstimulated


title: Tonsil stromal cells 6


title: peripheral blood buffy coat, S0224


title: NSP017-PT01


title: NSP016-PT01


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E3_ad002


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E2_ad002


title: Fibroblastic reticular cells 5


title: Fibroblastic reticular cells 1


title: peripheral blood buffy coat, S0228


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E2_ad001


title: NSP014-PT01


title: peripheral blood buffy coat, S0221


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E12_ad012


title: peripheral blood buffy coat, S0220


title: NSP013-PT01


title: peripheral blood buffy coat, S0217


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E12_ad010


title: NSP012-PT01


title: peripheral blood buffy coat, S0215


title: peripheral blood buffy coat, S0216


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E12_ad007


title: NSP010-PT01


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E12_ad006


title: NSP011-PT01


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E12_ad001


title: peripheral blood buffy coat, S0213


title: Tonsil stromal cells 1


title: NSP009-RM01

value: Metastasis

value: Yes


title: NSP008-RT01


title: NSP015-PT01


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E11_ad012


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E11_ad010


title: peripheral blood buffy coat, S0205


title: Dead cell sgRNA sequencing from KSHV infected cells

city: Seattle

first: Michael

last: Lagunoff

state: WA

address: 750 Republican St.

department: Microbiology

institute: University of Washington

description:
KSHV_Dead
processed data file: Dead_Cell_CRISPR_Screen.xlsx

data_processing:
Deconvolution
Read mapping and counting to library file to generate list of sgRNA counts per sample using MAGeCK.
Testing using MAGeCK to identify sgRNAs which are significantly depleted or enriched between sample populations.
Genome_build: Mapping was to an included library file, not a genome.
Supplementary_files_format_and_content: Count files and test result files are provided in Excel Tables.

source_name: TIME cells

growth_protocol: Grown in EBM2 with Bullet kit from Lonza, details in methods.

extract_protocol:
Qiagen Blood and Cell Culture DNA Maxi Kit.
PCR using primers flanking sgRNA encoding sequence in the CRISPR Lentivirus library, followed by gel purification.
Read 1 followed by index reading with a custom indexing primer.

treatment_protocol: CRISPR library transduced, followed by puromycin selection, then KSHV infection where indicated. Passaged for 8 days.

tag: cell line

value: TIME

tag: treatment

value: KSHV Infected

tag: cell status

value: Dead


title: NSP007-RM01

value: Neuroblastoma primary samples


title: Live cell sgRNA sequencing from KSHV infected cells

description:
KSHV_Live
processed data file: Live_Cell_CRISPR_Screen.xlsx

value: Live


title: NSP006-RM01

value: NonAmp


title: peripheral blood buffy coat, S0204


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E11_ad008

tag: brain region

tag: Sex


title: Dead cell sgRNA sequencing from uninfected cells

description:
Mock_Dead
processed data file: Dead_Cell_CRISPR_Screen.xlsx

value: Uninfected


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E11_ad007

value: DG


title: NSP005-RT01

tag: tissue

value: Tumor

tag: gender

value: M


title: Live cell sgRNA sequencing from uninfected cells

description:
Mock_Live
processed data file: Live_Cell_CRISPR_Screen.xlsx


title: CEMBA190625_10F_1_CEMBA190625_10F_2_E11_ad006

value: male


title: peripheral blood buffy coat, S0209

value: peripheral blood buffy coat

tag: art treatment status (0 = spontaneous; 1 = assisted reproduction)

tag: maternal age at delivery (years)


title: peripheral blood buffy coat, S0202

tag: pre-pregnancy bmi (kg/m^2)


title: peripheral blood buffy coat, S0203


title: NSP004-RT01

value: Amp