title: CEMBA181002_5C_7_CEMBA181002_5C_8_G3_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G3_ad007

status: Public on Sep 13 2020

type: SRA

city: La Jolla

first: Joseph

middle: R

last: Ecker

state: CA

address: 10010 North Torrey Pines Road

department: Genomic Analysis Laboratory

country: USA

institute: HHMI-Salk-Institute

library_source: genomic

data_processing:
Library strategy: snmC-seq2
Reads trimed with Cutadapt for adapters and low quality bases (Phred score >= 20)
The resulting files are mapped to in-silico bisulfite converted mm10 reference genome using Bismark.
The raw BAM file will be filtered using samtools to filter reads by MAPQ > 10 and use Picard to remove PCR duplicates
Tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls will be generated with in-house developing package using the filtered BAM files
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls. Columns are: 1. Chromosome, 2. Position, 3. Strand, 4. Cytosin Context, 5. methylated base call, 6. total mapped base call, 7. not used, place holder column

molecule: genomic DNA

organism: Mus musculus

source_name: C57BL/6J Male P56 Mouse Brain

growth_protocol: All mice are C57BL/6J Male, brain dissected in P56

extract_protocol:
the brain of P56 male C57BL/6J mouse will be removed and cut into 600 microns thick coronal sections using a tissue matrix. Then each region will be microdissected from the coronal tissue sections based on identified landmarks and brain atlas coordinates, as defined in Allen brain atlas CCF3 (Avaliable at: http://atlas.brain-map.org). For each dissection region, the tissue from several littermate mice will be combined together into to separate replicate groups. The number of animals in a replicate depends on the size of the tissue. Nuclei preparation will be stained with the fluorescently labeled anti-NeuN body and nucleic acid stain Hoechst 33342.
Library prepared following protocol discribed in Luo, Chongyuan, Angeline Rivkin, Jingtian Zhou, Justin P. Sandoval, Laurie Kurihara, Jacinta Lucero, Rosa Castanon, et al. 2018. “Robust Single-Cell DNA Methylome Profiling with snmC-seq2.” Nature Communications 9 (1): 3824.

tag: brain region

value: SSs

tag: Sex

value: male

tag: age

tag: strain


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G3_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G3_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G2_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G5_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G2_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G2_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G2_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G4_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G2_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G2_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G11_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G12_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G12_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G11_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G12_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G12_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G11_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G11_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G10_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G11_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G10_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G10_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G10_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G10_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G10_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G10_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G2_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G1_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G1_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G1_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G1_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G1_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G12_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G11_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G1_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F9_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_G11_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F9_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F9_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F9_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F8_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F8_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F8_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F9_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F7_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F8_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F9_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F8_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F7_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F7_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F7_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F7_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F6_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F7_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F6_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F7_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F6_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F7_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F6_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F6_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F5_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F5_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F5_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F4_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F5_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F4_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F4_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F5_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F8_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F4_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F5_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F3_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F3_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F2_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F3_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F2_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F2_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F3_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F4_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F2_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F2_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F12_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F12_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F12_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F12_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F11_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F12_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F11_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F11_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F2_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F11_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F11_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F2_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F11_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F10_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F11_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F11_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F10_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F10_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F10_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F10_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F1_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F1_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F1_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F1_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E9_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F10_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E9_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E9_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E9_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E9_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E8_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E8_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E8_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E8_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E8_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E7_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E7_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E7_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E7_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_F10_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E7_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E7_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E6_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E6_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E5_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E6_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E5_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E5_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E8_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E8_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E6_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E6_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E5_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E4_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E5_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E4_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E5_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E4_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E3_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E3_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E7_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E3_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E4_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E3_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E3_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E2_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E3_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E6_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E2_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E2_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E2_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E2_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E2_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E12_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E2_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E3_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E12_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E12_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E12_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E12_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E12_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E11_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E11_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E12_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E11_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E11_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E11_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E6_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E10_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E10_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E10_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E10_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E10_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E10_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E11_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E1_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E1_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E1_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E1_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E1_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E1_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D9_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D9_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D9_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D9_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D9_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D8_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_E10_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D9_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D8_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D8_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D8_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D7_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D7_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D7_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D7_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D6_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D6_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D7_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D7_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D6_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D6_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D6_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D5_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D5_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D8_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D5_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D6_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D6_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D4_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D4_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D4_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D5_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D7_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D4_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D3_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D4_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D3_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D3_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D3_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D4_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D3_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D2_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D2_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D2_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D2_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D2_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D4_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D2_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D5_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D3_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D3_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D12_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D12_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D12_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D12_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D2_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D12_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D12_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D11_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D11_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D3_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D12_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D11_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D11_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D11_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D10_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D11_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D10_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D10_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D10_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D10_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D10_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D1_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D1_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D1_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C9_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D1_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C9_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C9_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C9_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C9_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C8_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C8_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C9_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C8_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D10_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C7_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C9_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C7_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C7_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C7_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C7_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C6_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C7_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C6_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D1_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C6_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C5_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C5_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C5_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C5_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_D1_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C5_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C6_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C4_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C5_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C4_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C5_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C4_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C4_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C3_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C3_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C3_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C3_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C3_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C2_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C6_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C5_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C2_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C2_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C2_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C12_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C2_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C2_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C3_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C12_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C12_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C12_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C12_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C11_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C3_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C11_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C11_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C11_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C10_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C1_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C11_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C10_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C10_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C10_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C10_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C1_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C1_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C1_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C1_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B9_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C1_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B9_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C12_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C12_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C11_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C11_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C10_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B9_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B8_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B7_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B8_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B7_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B8_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B7_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B8_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B7_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B8_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B6_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B7_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B7_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B9_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_C4_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B6_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B6_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B9_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B6_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B6_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B5_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B5_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B6_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B5_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B9_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B5_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B5_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B5_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B7_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B7_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B5_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B4_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B5_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B4_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B4_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B4_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B4_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B3_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B3_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B4_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B3_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B3_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B2_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B3_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B2_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B3_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B2_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B12_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B2_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B2_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B2_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B12_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B12_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B2_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B12_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B12_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B12_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B3_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B11_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B11_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B11_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B11_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B2_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B11_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B11_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B10_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B10_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B11_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B10_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B1_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B10_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B10_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B1_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B1_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B10_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B4_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B1_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B1_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A9_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A9_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B1_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A9_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A9_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A8_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A8_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A9_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_B12_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A7_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A7_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A7_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A8_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A7_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A7_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A6_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A7_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A7_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A6_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A6_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A8_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A5_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A6_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A6_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A5_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A6_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A5_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A5_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A4_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A5_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A5_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A5_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A4_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A4_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A4_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A6_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A4_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A6_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A3_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A4_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A4_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A3_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A3_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A3_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A3_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A2_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A2_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A3_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A2_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A2_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A2_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A2_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A2_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A12_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A12_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A11_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A12_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A11_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A12_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A11_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A11_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A3_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A11_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A10_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A10_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A10_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A10_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A10_ad002


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A1_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A1_ad008


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A1_ad010


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A10_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A1_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H9_ad007


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A10_ad006


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A1_ad004


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A1_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H9_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H9_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H9_ad001


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A12_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H9_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H9_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H9_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H8_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H8_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H8_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H8_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H7_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H8_ad012


title: CEMBA181002_5C_7_CEMBA181002_5C_8_A1_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H7_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H8_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H7_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H7_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H7_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H7_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H7_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H6_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H6_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H6_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H6_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H6_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H6_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H5_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H5_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H5_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H5_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H4_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H4_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H5_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H4_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H4_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H4_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H4_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H4_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H3_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H3_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H3_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H3_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H3_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H2_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H2_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H2_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H2_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H2_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H2_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H12_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H12_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H12_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H11_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H12_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H10_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H10_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H3_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H10_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H10_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H10_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G9_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G9_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G9_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G8_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H12_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G8_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G8_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G8_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H11_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G7_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G8_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H11_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G7_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G7_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G7_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G7_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G6_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G7_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H11_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G7_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H10_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G7_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G9_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H11_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G6_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G6_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G6_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G6_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G6_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G5_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G5_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G5_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G5_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G4_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G5_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G5_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G4_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G4_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G6_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G3_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G3_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G3_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G12_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G4_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G3_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G3_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G6_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G4_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G12_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G12_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G12_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G11_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G12_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G12_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G12_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G12_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G11_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_H12_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G5_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G11_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G10_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G11_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G10_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G10_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G10_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G3_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G1_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G10_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G1_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G1_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F9_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G1_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G1_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F9_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F9_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F9_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F9_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F8_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G1_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F9_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F8_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F7_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F7_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F8_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F7_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F7_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F6_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F6_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F6_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F7_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F7_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_G1_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F6_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F5_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F5_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F6_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F8_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F5_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F5_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F4_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F8_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F5_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F5_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F4_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F4_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F4_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F7_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F6_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F3_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F3_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F3_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F4_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F3_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F3_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F2_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F2_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F2_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F2_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F5_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F2_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F6_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F12_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F12_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F12_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F11_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F12_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F12_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F11_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F3_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F3_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F11_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F10_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F11_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F10_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F11_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F10_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F10_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F10_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F10_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F10_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F1_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F1_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F1_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F1_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F1_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F12_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F1_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E9_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E9_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E9_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F11_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E9_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E8_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E8_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E8_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F10_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E8_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E8_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E9_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_F12_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E7_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E7_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E7_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E8_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E7_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E6_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E6_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E6_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E6_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E6_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E7_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E6_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E6_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E7_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E5_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E5_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E7_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E5_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E5_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E4_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E5_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E4_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E4_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E4_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E4_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E4_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E7_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E3_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E4_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E3_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E3_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E3_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E3_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E3_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E3_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E2_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E2_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E5_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E12_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E2_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E2_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E2_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E2_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E12_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E12_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E12_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E12_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E11_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E11_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E11_ad008


title: h11_1: PGCs_11hpf_repeat1

city: Shanghai

first: XiaoYuan

last: Zhang

address: 320 Yueyang road

country: China

institute: Shanghai Institute of Biochemistry and Cell Biology,CAS

library_source: transcriptomic

data_processing:
Raw reads were pre-processed by FASTX-Toolkit version 0.0.14-1
Reads were mapped to danRer11 using bowtie version 1.0.0-5
miRNAs were quantified based on miRBase version 22.0
piRNAs were quantified using proTRAC version 2.2.0
Genome_build: danRer11
Supplementary_files_format_and_content: tab-delimited text file includes read number and RPM of miRNA expression for each sample …

molecule: total RNA

organism: Danio rerio

source_name: PGCs

extract_protocol:
PGCs were picked out by green fluoresence
small RNA-seq library was constructed by microscale small RNA high-throughput sequencing method.

tag: cell type

value: primordial germ cells

value: AB

tag: tissue

value: gonad


title: h11_2: PGCs_11hpf_repeat2


title: h24_2: PGCs_24hpf_repeat2


title: h24_1: PGCs_24hpf_repeat1


title: h6_2: PGCs_6hpf_repeat2


title: P3_11c_43

city: Cambridge

first: Davide

last: Chiarugi

address: Box 289, Addenbrooke’s Hospital

department: Metabolic Research Laboratories

country: United Kingdom

institute: WT-MRC Institute of Metabolic Science

description: genomic DNA from Suspension HeLa-Cas9 cells transfected with Brunello library P3_11c_43

data_processing:
Library strategy: DNA Sequencing
The fastq files were combined based on PCR replicates and were processed using MaGECK to generate sgRNA counts. Brunello sgRNA library was used as the reference for counting
Supplementary_files_format_and_content: Data table and Brunello sgRNA Library provided in MS Excel format

organism: Homo sapiens

extract_protocol:
DNA purification using Gentra Puregene cell kit, 1-2 x 10^7 protocol
Crispr Screening via PCR of sgRNAs according to protocol by Doench et al., DOI: 10.1038/nbt.3437

treatment_protocol: Hela-Cas9 cells were transfected with Brunello library

tag: cell line

tag: sort id

value: Sort 3, positive

tag: library description

value: Galectin-3 positive population after sort 3 (highest 1 %), sample c

tag: days post sort

tag: collection replicate

tag: pcr replicate


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E11_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E11_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E11_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E10_ad012


title: h6_1: PGCs_6hpf_repeat1


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E10_ad008


title: P3_11c_31

description: genomic DNA from Suspension HeLa-Cas9 cells transfected with Brunello library P3_11c_31


title: P3_11c_19

description: genomic DNA from Suspension HeLa-Cas9 cells transfected with Brunello library P3_11c_19


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E12_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E10_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E12_ad006


title: BJ2

first: Jian

last: Bing

address: Songhu Road, Yangpu District

department: School of Life Sciences

institute: Fudan University

data_processing:
Illumina OLB_1.9.4 were used for basecalling
Filter dirty raw reads by removing reads with adaptors and low quality, the obtained clean reads were mapped to reference sequences using SOAPaligner/soap2 according to Li et al., Bioinformatics, 2009. Mismatches no more than 2 bases were allowed in the alignment.
The gene expression level is calculated by using FPKM method (Reads Per kb per Million reads) according to Mortazavi, et a., Nature Methods, 2008, and the formula is shown as follows: FPKM=106C/(NL/103). C is the number of reads that uniquely aligned to a gene, N is total number of reads that uniquely aligned to all genes, and L is the number of bases on a gene .
Genome_build: Candida auris genome
Supplementary_files_format_and_content: text files containing FPKM values for each Sample

organism: Candidozyma auris

source_name: Candida auris

growth_protocol: YPD medium and cultured at 30 oC for 12h.

extract_protocol:
Cells were lysed by Mini-beadbeater and total RNA was harvested using GeneJET RNA Purification Kit (Thermo Scientific #K0731, #K0732)
RNA libraries were prepared for sequencing using standard Illumina protocols

tag: genotype

value: Wild type


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E10_ad007


title: P3_11c_07

description: genomic DNA from Suspension HeLa-Cas9 cells transfected with Brunello library P3_11c_07


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E10_ad004


title: BJ3


title: BJ1


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E10_ad002


title: P3_11b_90

description: genomic DNA from Suspension HeLa-Cas9 cells transfected with Brunello library P3_11b_90

value: Galectin-3 positive population after sort 3 (highest 1 %), sample b


title: B724-3

value: Diploid


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E10_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E1_ad012


title: P3_11b_78

description: genomic DNA from Suspension HeLa-Cas9 cells transfected with Brunello library P3_11b_78


title: B724-2


title: P3_11b_66

description: genomic DNA from Suspension HeLa-Cas9 cells transfected with Brunello library P3_11b_66


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E1_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E1_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E10_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E1_ad006


title: B476-3

value: Haploid


title: B724-1


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E1_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E1_ad010


title: P3_11b_54

description: genomic DNA from Suspension HeLa-Cas9 cells transfected with Brunello library P3_11b_54


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E1_ad002


title: P3_11a_42

description: genomic DNA from Suspension HeLa-Cas9 cells transfected with Brunello library P3_11a_42

value: Galectin-3 positive population after sort 3 (highest 1 %), sample a


title: B476-2


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D9_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D9_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_E1_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D9_ad007


title: B476-1


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D9_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D9_ad006


title: P3_11a_18

description: genomic DNA from Suspension HeLa-Cas9 cells transfected with Brunello library P3_11a_18


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D9_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D9_ad004


title: WT2 minus Dox, NPCs, day2, rep3

city: Rehovot

first: Igor

last: Ulitsky

address: Hertzl St.

country: Israel

institute: Weizmann Institute of Science

description: Day2 Rescue WTmDox 3

data_processing:
Quantification using RSEM
Alignment to the mm9 mouse genome using STAR2
Preparation of bigWig files using wigToBigWIg
Genome_build: mm9
Supplementary_files_format_and_content: RSEM quantification files and bigWig read coverage

molecule: polyA RNA

source_name: NPCs

growth_protocol: R1 mouse ES cells were grown on a feeder layer of irradiated mouse embryonic fibroblasts (MEFs) on 0.1% gelatin-coated cell culture dishes. Culture medium consisted of DMEM (Gibco), 15% ES-grade fetal calf serum (FCS, Biological Industries), 1X Glutamax (Gibco), 1 mM sodium pyruvate (Gibco), 0.1 mM nonessential amino acids (Gibco), 0.1 mM β-mercaptoethanol (Sigma), 100 U/ml penicillin and 0.1 mg/ml streptomycin (Biological Industries), and 1000 U/ml LIF. Neuronal differentiation was performed as previously described (Ying et al., 2003). Pluripotent stem cells were first grown in the absence of MEFs for two passages and then seeded on gelatin-coated plates at a density of 2.1*104/cm2 in N2B27 medium: 1:1 mixture of DMEM/F12 (Sigma) supplemented with N2 (Gibco), and Neurobasal medium (Gibco) supplemented with B27 (Gibco), 1X Glutamax (Gibco) ), 0.1 mM β-mercaptoethanol (Sigma), 100 U/ml penicillin, and 0.1 mg/ml streptomycin (Biological industries). After four days under these conditions, the cells were dissociated and re-plated on plates coated with Poly-D-Lysine (Sigma) and Laminin (Sigma), at a density of 3.7*104/cm2 in N2B27 medium supplemented with 20ng/ml FGF2 (Peprotech). After 24 hr, FGF2 was removed from medium and cells were grown for three additional days.

extract_protocol:
Total RNA was extracted from cells, using the Trizol reagent (TRI, Sigma-Aldrich) according to the manufacturer’s protocol.
Lexogen  SENSE-mRNA-Seq

treatment_protocol: Short sequences (21nt) complementary to target genes were synthetized as 58nt single-stranded oligos as forward oligo: 5’-CCGG—21nt sense—CTCGAG—21nt antisense—TTTTTG-3’, and reverse oligo: 5’-AATTCAAAAA—21nt sense—CTCGAG—21nt antisense-3’. Forward and reverse oligos were phosphorylated and annealed to each other by the addition of T4 ligation buffer (NEB) and T4 PNK (NEB) and incubation at 37°C for 30 minutes followed by incubation at 95°C for five minutes and gradual cooling to room temperature at a rate of 5ºC/min. The annealed oligo pairs were ligated to pLKO.1 vector (Sigma # SHC001) which was digested by AgeI and EcoRI. Ligated plasmids were transformed into NEB stable competent bacteria. A plasmid containing an shRNA not targeting a mammalian sequence (Sigma #SHC002) was used as negative control.  To generate Reno m/m ES cells we deleted the promoter and first exon of Reno (a total of 631bp) by transfection of pCas9_GFP plasmid (Addgene #44719) and two gRNA plasmids. To generate Bahcc1 m/m cells we created short deletions containing the start codon of Bahcc1, thereby inhibiting its translation, by transfection of pCas9_GFP plasmid (Addgene #44719) and one gRNA plasmid targeting Bahcc1 start codon.

value: NPCs

tag: time

value: day 2


title: Renonm/m2 plus Dox, NPCs, day2, rep3

description: Day2 Rescue KOpDox 3


title: P2_8a_88

description: genomic DNA from Suspension HeLa-Cas9 cells transfected with Brunello library P2_8a_88

value: Sort 2, positive

value: Galectin-3 positive population after sort 2 (highest 2 %), sample a


title: P3_11a_06

description: genomic DNA from Suspension HeLa-Cas9 cells transfected with Brunello library P3_11a_06


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D9_ad008


title: P2_8a_76

description: genomic DNA from Suspension HeLa-Cas9 cells transfected with Brunello library P2_8a_76


title: Renonm/m2 minus Dox, NPCs, day2, rep2

description: Day2 Rescue KOmDox 2


title: P2_8a_64

description: genomic DNA from Suspension HeLa-Cas9 cells transfected with Brunello library P2_8a_64


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D8_ad010


title: Renonm/m2 plus Dox, NPCs, day2, rep2

description: Day2 Rescue KOpDox 2


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D8_ad012


title: WT2 plus Dox, NPCs, day2, rep3

description: Day2 Rescue WTpDox 3


title: P3_11a_30

description: genomic DNA from Suspension HeLa-Cas9 cells transfected with Brunello library P3_11a_30


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D8_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D7_ad007


title: P1_8a_86

description: genomic DNA from Suspension HeLa-Cas9 cells transfected with Brunello library P1_8a_86

value: Sort 1, positive

value: Galectin-3 positive population after sort 1 (highest 10 %), sample a


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D7_ad012

value: SSs

value: male


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D7_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D7_ad008


title: P1_8a_74

description: genomic DNA from Suspension HeLa-Cas9 cells transfected with Brunello library P1_8a_74


title: P2_8a_52

description: genomic DNA from Suspension HeLa-Cas9 cells transfected with Brunello library P2_8a_52


title: WT2 plus Dox, NPCs, day2, rep2

description: Day2 Rescue WTpDox 2


title: WT2 minus Dox, NPCs, day2, rep2

description: Day2 Rescue WTmDox 2


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D7_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D6_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D6_ad010


title: P1_8a_62

description: genomic DNA from Suspension HeLa-Cas9 cells transfected with Brunello library P1_8a_62


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D6_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D6_ad007


title: P1_8a_50

description: genomic DNA from Suspension HeLa-Cas9 cells transfected with Brunello library P1_8a_50


title: Renonm/m2 minus Dox, NPCs, day2, rep1

description: Day2 Rescue KOmDox 1


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D6_ad004


title: WT2 minus Dox, NPCs, day2, rep1

description: Day2 Rescue WTmDox 1


title: Renonm/m2 plus Dox, NPCs, day2, rep1

description: Day2 Rescue KOpDox 1


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D6_ad002


title: P1_0a_38

description: genomic DNA from Suspension HeLa-Cas9 cells transfected with Brunello library P1_0a_38


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D6_ad008


title: P1_0a_26

description: genomic DNA from Suspension HeLa-Cas9 cells transfected with Brunello library P1_0a_26


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D5_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D8_ad002


title: WT2 plus Dox, NPCs, day2, rep1

description: Day2 Rescue WTpDox 1


title: Reno Gapmer1, NPCs, day 2, rep3

description: Day2 Gapmers Reno1 3


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D5_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D5_ad004


title: P1_0a_14

description: genomic DNA from Suspension HeLa-Cas9 cells transfected with Brunello library P1_0a_14


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D5_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D5_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D4_ad012


title: Scrambled Gapmer, NPCs, day 2, rep3

description: Day2 Gapmers Scr 3


title: U1_0a_48

description: genomic DNA from Suspension HeLa-Cas9 cells transfected with Brunello library U1_0a_48

value: Sort 1, unsorted control

value: Unsorted population, collected after sort 1


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D4_ad007


title: Reno Gapmer2, NPCs, day 2, rep2

description: Day2 Gapmers Reno2 2


title: U1_0a_37

description: genomic DNA from Suspension HeLa-Cas9 cells transfected with Brunello library U1_0a_37


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D3_ad012


title: U1_0a_36

description: genomic DNA from Suspension HeLa-Cas9 cells transfected with Brunello library U1_0a_36


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D4_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D4_ad006


title: Scrambled Gapmer, NPCs, day 2, rep2

description: Day2 Gapmers Scr 2


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D3_ad001


title: Reno Gapmer2, NPCs, day 2, rep1

description: Day2 Gapmers Reno2 1


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D3_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D3_ad004


title: P1_0a_02

description: genomic DNA from Suspension HeLa-Cas9 cells transfected with Brunello library P1_0a_02


title: Bahcc1 shRNAs, NPCs, day 2, rep2

description: Day2 shRNAs Bahcc1 1+2 2


title: U1_0a_24

description: genomic DNA from Suspension HeLa-Cas9 cells transfected with Brunello library U1_0a_24


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D3_ad010


title: Reno Gapmer1, NPCs, day 2, rep1

description: Day2 Gapmers Reno1 1


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D4_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D4_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D3_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D3_ad008


title: U1_0a_13

description: genomic DNA from Suspension HeLa-Cas9 cells transfected with Brunello library U1_0a_13


title: U1_0a_12

description: genomic DNA from Suspension HeLa-Cas9 cells transfected with Brunello library U1_0a_12


title: Reno Gapmer1, NPCs, day 2, rep2

description: Day2 Gapmers Reno1 2


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D2_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D2_ad012


title: U1_0a_01

description: genomic DNA from Suspension HeLa-Cas9 cells transfected with Brunello library U1_0a_01


title: Reno shRNAs, NPCs, day 2, rep2

description: Day2 shRNAs Reno 1+2 2


title: Reno shRNAs, NPCs, day 2, rep1

description: Day2 shRNAs Reno 1+2 1


title: NT shRNA, NPCs, day 2, rep2

description: Day2 shRNAs control 2


title: Sham operation group rats_9A

city: Baise

first: huang

last: qing

address: No.18, Zhongshan 2nd Road, Youjiang District

institute: Youjiang Medical University for Nationalities

data_processing:
Reads obtained from the sequencing machines includes raw reads containing adapters or low quality bases which will affect the following assembly and analysis. Thus, to get high quality clean reads, reads were further filtered by fastp (version 0.18.0). The parameters were as follows:1) removing reads containing adapters;2) removing reads containing more than 10% of unknown nucleotides (N);3) removing low quality reads containing more than 50% of low quality (Q-value≤20) bases.
Short reads alignment tool Bowtie2 (version 2.2.8) was used for mapping reads to ribosome RNA (rRNA) database. The rRNA mapped reads then will be removed. The remaining clean reads were further used in assembly and gene abundance calculation.
An index of the reference genome was built, and paired-end clean reads were mapped to the reference genome using HISAT2. 2.4 with “-rna-strandness RF” and other parameters set as a default.
The mapped reads of each sample were assembled by using StringTie v1.3.1 in a reference-based approach. For each transcription region, a FPKM (fragment per kilobase of transcript per million mapped reads) value was calculated to quantify its expression abundance and variations, using StringTie software.

organism: Rattus norvegicus

source_name: Brain tissue

extract_protocol:
Total RNA was extracted using Trizol reagent kit (Invitrogen, Carlsbad, CA,USA) according to the manufacturer’s protocol. RNA quality was assessed on an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA) and checked using RNase free agarose gel electrophoresis. After total RNA was extracted, eukaryotic mRNA was enriched by Oligo(dT) beads, while prokaryotic mRNA was enriched by removing rRNA by Ribo-ZeroTM Magnetic Kit (Epicentre, Madison, WI, USA). Then the enriched mRNA was fragmented into short fragments using fragmentation buffer and reverse transcripted into cDNA with random primers. Second-strand cDNA were synthesized by DNA polymerase I, RNase H, dNTP and buffer. Then the cDNA fragments were purified with QiaQuick PCR extraction kit(Qiagen, Venlo, The Netherlands), end repaired, poly(A) added, and ligated to Illumina sequencing adapters. The ligation products were size selected by agarose gel electrophoresis, PCR amplified, and sequenced using Illumina HiSeq2500 by Gene Denovo Biotechnology Co. (Guangzhou, China)
RNA libraries were prepared for sequencing using standard Illumina protocols

value: brain

tag: treatment

value: Sham operation group


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D2_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D2_ad002


title: U1_0a_25

description: genomic DNA from Suspension HeLa-Cas9 cells transfected with Brunello library U1_0a_25


title: Sham operation group rats_4B


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D2_ad010


title: NT shRNA, NPCs, day 2, rep1

description: Day2 shRNAs control 1


title: Panax notoginseng for MCAO in rats_7A

value: Panax notoginseng for MCAO


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D2_ad008


title: Bahcc1 shRNAs, NPCs, day 2, rep1

description: Day2 shRNAs Bahcc1 1+2 1


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D12_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D12_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D12_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D12_ad004


title: 2081L_S71

city: Corvallis

first: Andrey

last: Morgun

state: OR

address: 1601 SW Jefferson Way

institute: Oregon State University

description:
2081L_S71
processed data file: rel_mill.txt

data_processing:
De-multiplexed sequencing reads were generated using Illumina bcl2fastq (released version 2.18.0.12) allowing no mismatches in the index read.
Sequences were processed to remove adapter, polyA and low-quality bases by BBTools (https://jgi.doe.gov/data-and-tools/bbtools/).
Reads were aligned to mouse genome and transcriptome using Tophat (v2.1.1).
Number of reads per million for mouse genes were counted using HTSeq (v 0.6.0) and normalized per million.
Genome_build: ENSEMBL NCBIM37
Supplementary_files_format_and_content: rel_mill.txt: Tab-delimited text file includes relativized counts per million values for each Sample.

extract_protocol:
RNA was extracted using an OMNI Bead Ruptor and 2.8 mm ceramic beads (OMNI International) in RLT buffer followed by Qiashredder and RNeasy kit using Qiacube (Qiagen) automated extraction according to manufacturer’s specifications. Total RNA was quantified using Quant-iT RNA Assay Kit (Thermo Fisher Scientific). Complementary DNA was prepared using qScript reverse transcription kit (Bio-Rad) and qPCR was performed using Perfecta SYBR mix (Quantabio) and StepOne Plus Real Time PCR system and software (Applied Biosystems).
RNA libraries were prepared with QuantSeq 3’mRNA-Seq Library Prep Kit (Lexogen) for the Apollo 324 NGS Library Prep System.
Illumina NextSeq 500; 75 bp single-end read.

tag: mouse id

tag: diet

tag: group

value: Liver

tag: liver

tag: ileum

tag: expt

value: Experiment 3

tag: numb


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D12_ad006

tag: brain region

tag: Sex

tag: age

tag: strain


title: 2083L_S72

description:
2083L_S72
processed data file: rel_mill.txt

tag: tissue


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D2_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D12_ad002


title: Panax notoginseng for MCAO in rats_6A


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D2_ad007


title: Sham operation group rats_5B


title: Middle cerebral artery occlusion in rats_7A

value: Middle cerebral artery occlusion


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D12_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D10_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D10_ad010


title: Middle cerebral artery occlusion in rats_6B


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D11_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D10_ad007


title: 2074L_S66

description:
2074L_S66
processed data file: rel_mill.txt


title: 2078L_S69

description:
2078L_S69
processed data file: rel_mill.txt


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D10_ad004


title: Middle cerebral artery occlusion in rats_3B


title: 2075L_S67

description:
2075L_S67
processed data file: rel_mill.txt


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D10_ad002


title: Exit - Ungated

first: Celine

last: Labouesse

address: Puddicombe Way

department: Cambridge Stem Cell Institute

institute: University of Cambridge

description:
24 single cells
counts_SLX15496.txt

data_processing:
Read trimming: Trim Galore v 0.4.4_dev for Trimming of reads to remove Nextera Adapter sequence: 'CTGTCTCTTATA' and remove low-quality reads & too short reads
Read alignment: GSNAP version 2015-09-29 for Alignment of genome to genome build mm10
Read mapping: HTSeq; Genes were annotated using Ensembl release 81 and read counts were quantified using HTSeq
Quality control: R packages: scran
Excluding cell with total counts < 1.3 e6  Excluding cells with total features < 7000  Excluding cells with %ERCC <= 25 %
Count normalisation: R package; DESeq2, Cyclone (scran)
Used package "cyclone" for cell cycle phase assignment; Used DESeq2 for normalisation of raw counts and subsequent analysis
Genome_build: mm10
Supplementary_files_format_and_content: counts_SLX15495.txt: raw counts (output from mapping, prior to quality control) for plate SLX15495 in a tab-separated txt format; rows are Ensembl Gene Ids, columns are single cells (given by barcodes)
Supplementary_files_format_and_content: counts_SLX15496.txt:raw counts (output from mapping, prior to quality control) for plate SLX15496 in a tab-separated txt format; rows are Ensembl Gene Ids, columns are single cells (given by barcodes)

source_name: embryonic stem cells

growth_protocol: mES cells grown in 2i prior to sort, and in 2i or N2B27 after size-based sorting on laminin-coated plates.

extract_protocol: Following SmartSeq2 protocol for single-cell RNAseq

treatment_protocol: Size-based cell sorting: gate for small cells (exit of mitosis and G1) was defined using forward and side scatter. An ungated population was used as control. Relation between size gate and cell cycle was confirmed in an independent experiment using cells expressing fluorescent reporters of cell cycle phase (FUCCI)

tag: cell type

value: embryonic stem cells

tag: medium

tag: sorting gate

value: ungated


title: 2080L_S70

description:
2080L_S70
processed data file: rel_mill.txt


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D1_ad012


title: Exit - Small

description:
24 single cells
counts_SLX15496.txt

value: small cell


title: Panax notoginseng for MCAO in rats_3A


title: 2073L_S65

description:
2073L_S65
processed data file: rel_mill.txt


title: 2071L_S64

description:
2071L_S64
processed data file: rel_mill.txt

value: HFHS+PBS


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D1_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D10_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D1_ad002


title: Naïve - Ungated

description:
24 single cells
counts_SLX15495.txt


title: 2070L_S63

description:
2070L_S63
processed data file: rel_mill.txt


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C9_ad012


title: Naïve - Small

description:
24 single cells
counts_SLX15495.txt


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D1_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D1_ad001


title: 2076L_S68

description:
2076L_S68
processed data file: rel_mill.txt


title: 2069L_S62

description:
2069L_S62
processed data file: rel_mill.txt


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C9_ad010


title: 2066L_S61

description:
2066L_S61
processed data file: rel_mill.txt


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D3_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_D10_ad006


title: Stat3_shLrrc31-2_Input

city: Singapore

first: Chadi

last: El Farran

address: 61 Biopolis Drive

country: Singapore

institute: Institute of Molecular and Cell Biology

data_processing:
The libraries were mapped using STAR aligner to mm9 genome, allowing up to 3 mismatches in each read and discarding reads that map to more than one locus. The options –alignIntronMax and –alignEndsType were set to 1 and EndToEnd respectively.
Tag Directories were created using makeTagDirectory of HOMER
Peaks were called using findPeaks module of HOMER
Genome_build: mm9
Supplementary_files_format_and_content: Tab-delimited text file containing information regarding the binding sites of Stat3

source_name: E14 mESCs

growth_protocol: E14 mouse ES cells were cultured on gelatin (0.1%) coated plates in mESC medium at 37°C with 5% CO2. The medium composition: 15% heat-inactivated ES-qualified fetal bovine serum (GIBCO), 2mM L-glutamine (GIBCO), 0.1mM nonessential amino acid (NEAA, GIBCO), 5000U/ml penicillin/streptomycin (GIBCO), 0.055mM β-mercaptoethanol (GIBCO) and 1000U/ml home-made leukemia inhibitory factor in Dulbecco’s modified Eagles’ medium (DMEM, Hyclone).

extract_protocol:
ChIP was performed as described previously (Loh et al., 2006). Briefly, ten million cells were cross-linked with 1% formaldehyde for 10 minutes at room temperature; formaldehyde was then quenched by the addition of 200 mM glycine and cells were washed once with ice-cold PBS.  Cell lysis was carried out with a lysis buffer containing 10 mM Tris-Cl (pH 8), 100 mM NaCl, 10 mM EDTA, 0.25% Triton X-100 and protease inhibitor cocktail (Roche). Cells were then resuspended in 1% SDS lysis buffer containing 50 mM HEPES-KOH (pH 7.5), 150 mM NaCl, 1% SDS, 2 mM EDTA, 1% Triton X-100, 0.1% NaDOC and protease inhibitor cocktail. The suspension was nutated for 15 minutes in the cold room before spinning down to collect the chromatin pellet. The pellet was then washed two times with 0.1% SDS lysis buffer containing 50 mM HEPES-KOH (pH 7.5), 150 mM NaCl, 0.1% SDS, 2 mM EDTA, 1% Triton X-100, 0.1% NaDOC, 1 mM PMSF and protease inhibitor cocktail. Sonication was conducted on the ice, using the Branson Sonifier 250 (11 cycles, power amplitude of 35%, 30 sec pulses on with 59.9 s pulses off). The chromatin solution was clarified by centrifugation at 20,000 g at 4°C for 45 minutes and then pre-cleared with Dynabeads protein A (Life Technologies) for 2 hours at 4°C. The pre-cleared chromatin sample was incubated with 50 μl of Dynabeads protein A loaded with 5 μg antibody overnight at 4°C. The beads were washed three times with 0.1% SDS lysis buffer, once with 0.1% SDS lysis buffer/0.35 M NaCl, once with 10 mM Tris-Cl (pH 8)/1 mM EDTA/0.5% NP40/0.25% LiCl/0.5% NaDOC, and once with TE buffer (pH8.0). The immunoprecipitated material was eluted from the beads by heating for 45 minutes at 68°C in 50 mM Tris-Cl (pH 7.5), 10 mM EDTA, 1% SDS. To reverse the crosslinks, samples were incubated with 1.5 μg/ml Pronase at 42°C for 2 hr followed by 67°C for 6 hr. The samples were then extracted with phenol-chloroform-isoamyl alcohol (25:24:1) followed by chloroform, ethanol precipitated in the presence of glycogen, and re-suspended in TE buffer.
ChIP-seq libraries were prepared following previously published protocol (Yang et al., 2015). Briefly, ChIP DNA was end-repaired. Then poly-A tail was added followed by ligation with adaptors. Finally, the fragments were amplified using 12 cycles of PCR. The amplified ChIP DNA was purified using Ampure beads. The concentration and size range of ChIP-seq library was measured by Agilent 2000 bioanalyzer kit

treatment_protocol: Dharmacon siDESIGN center was used for the design of the shRNA sequences (http://dharmacon.gelifesciences.com/design-center/). shRNA sequences were ordered as DNA oligos from IDT and cloned into pSUPER-puro plasmid. mESCs were trypsinized for 5 minutes at 37 °C, counted by hemocytometer and 200,000 cells seeded into one well of 6 well plates. mESCs were transfected with 2.5 μg of shRNA construct and 5 μl of Lipofectamine 2000 (Thermofisher). 1 μg/ml of puromycin was added to the mES medium 24 hours after transfection. All the knockdown experiments were carried out in biological triplicates.

tag: genotype/variation

value: shLrrc31 Construct 2

tag: antibody

value: E14 mESCs


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C9_ad006


title: 1998L_S60

description:
1998L_S60
processed data file: rel_mill.txt

tag: mouse id

value: Experiment 1


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C9_ad002

value: SSs


title: Stat3_shStat3_Input


title: Stat3_shLrrc31-1_Input

value: shLrrc31 Construct 1


title: 1997L_S59

description:
1997L_S59
processed data file: rel_mill.txt

tag: diet


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C9_ad008

value: male


title: Stat3_shEV_Input

value: WT


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C8_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C9_ad001


title: Stat3_shLrrc31-2

value: Anti-STAT3 (ab31370)


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C8_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C8_ad012


title: Stat3_shLrrc31-1


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C7_ad012


title: 1995L_S57

description:
1995L_S57
processed data file: rel_mill.txt

tag: group


title: 1993L_S56

description:
1993L_S56
processed data file: rel_mill.txt

tag: liver


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C7_ad006


title: Stat3_shStat3


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C7_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C7_ad004


title: 1991L_S54

description:
1991L_S54
processed data file: rel_mill.txt

tag: ileum


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C7_ad008


title: Stat3_shEV


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C9_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C6_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C8_ad010


title: 1996L_S58

description:
1996L_S58
processed data file: rel_mill.txt

tag: expt


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C6_ad004


title: 1990L_S53

description:
1990L_S53
processed data file: rel_mill.txt

tag: numb


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C6_ad002

tag: brain region


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C6_ad008

tag: Sex


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C7_ad001

tag: age


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C5_ad001

tag: strain


title: 1986L_S50

description:
1986L_S50
processed data file: rel_mill.txt

tag: tissue


title: 1988L_S51

description:
1988L_S51
processed data file: rel_mill.txt


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C5_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C5_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C5_ad004


title: 1985L_S49

description:
1985L_S49
processed data file: rel_mill.txt


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C6_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C4_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C5_ad008


title: 1992L_S55

description:
1992L_S55
processed data file: rel_mill.txt


title: 2081i_S29

description:
2081i_S29
processed data file: rel_mill.txt

value: Ileum


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C4_ad006


title: 2083i_S30

description:
2083i_S30
processed data file: rel_mill.txt


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C4_ad007


title: SW-1990_sh-PRLR_biological rep3

type: RNA

first: PeiQi

last: Huang

address: 800 Dongchuan Road

institute: Shanghai Cancer Institute

description: Gene expression data from biological replicate 3 of SW1990 cells infected with shRNA targeting PRLR

hyb_protocol: Array hybridization and wash was performed using GeneChip® Hybridization, Wash and Stain Kit (Cat#900720, Affymetrix, Santa Clara, CA, US)in Hybridization Oven 645 (Cat#00-0331-220V, Affymetrix, Santa Clara, CA, US)and Fluidics Station 450 (Cat#00-0079, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions.

scan_protocol: Slides were scanned by GeneChip® Scanner 3000 (Cat#00-00212, Affymetrix, Santa Clara, CA, US) and Command Console Software 4.0 (Affymetrix, Santa Clara, CA, US) with default settings.

data_processing: Raw data were normalized by MAS 5.0/RMA algorithm, Affy packages in R.

label: biotin

label_protocol: Total RNA were amplified, labeled and purified by using GeneChip  3' IVT PLUS Reagent Kit (Cat#902416, Affymetrix, Santa Clara, CA, US) / Ovation FFPE WTA System (Cat#3403， NuGEN, San Carlos, CA, US) / Ovation® Pico WTA System V2(Cat#3302， NuGEN, San Carlos, CA, US)and FL-Ovation™ cDNA Biotin Module V2(Cat#4200， NuGEN, San Carlos, CA, US) followed the manufacturer’s instructions to obtain biotin labeled cRNA.

growth_protocol: Cells were cultured accroding to American Type Culture Collection (ATCC, Manassas, VA) protocols.

extract_protocol: Trizol extraction of total RNA was performed according to the manufacturer's instructions.

treatment_protocol: Wildtype SW-1990 cells were infected with reconstructed lentivirus expressing shRNA and treated with 1 μg/ml puromycin for more than 7 days.

value: pancreas; Derived from metastatic site: spleen

tag: cell line

tag: treatment

value: shRNA targeting PRLR


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C4_ad002


title: SW-1990_sh-PRLR_biological rep1

description: Gene expression data from biological replicate 1 of SW1990 cells infected with shRNA targeting PRLR


title: SW-1990_sh-Ctrl_biological rep3

description: Gene expression data from biological replicate 3 of SW1990 cells infected with scrambles shRNA

value: scrambled shRNA


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C4_ad012


title: 1989L_S52

description:
1989L_S52
processed data file: rel_mill.txt

value: Liver


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C4_ad004


title: SW-1990_sh-PRLR_biological rep2

description: Gene expression data from biological replicate 2 of SW1990 cells infected with shRNA targeting PRLR


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C4_ad008


title: 2080i_S28

description:
2080i_S28
processed data file: rel_mill.txt

value: Experiment 3


title: LD_7

city: Valenzano-Bari

first: Sharon

middle: Natasha

last: Cox

department: Renal Lab

country: Italy

institute: Schena Foundation

hyb_protocol: Standard Illumina hybridization protocol.

scan_protocol: Standard Illumina scanning protocol.

data_processing: The data were normalised using quantile normalisation with Illumina GenomeStudio.

source_name: LD

label_protocol: Biotinylated cDNA was prepared with the Illumina WG-DASL asay.

extract_protocol: RNA was extracted with QIAGEN miRNeasy FFPE kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.

value: f

tag: subject status

value: living donors (LD)

value: FFPE_Kidney_biopsy specimen


title: LD_6


title: 2078i_S27

description:
2078i_S27
processed data file: rel_mill.txt


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C3_ad012


title: 2076i_S26

description:
2076i_S26
processed data file: rel_mill.txt


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C3_ad008


title: 2074i_S24

description:
2074i_S24
processed data file: rel_mill.txt


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C2_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C2_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C2_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C3_ad007


title: 2075i_S25

description:
2075i_S25
processed data file: rel_mill.txt


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C3_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C2_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C2_ad008


title: 2073i_S23

description:
2073i_S23
processed data file: rel_mill.txt


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C12_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C2_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C12_ad010


title: SW-1990_sh-Ctrl_biological rep2

description: Gene expression data from biological replicate 2 of SW1990 cells infected with scrambles shRNA


title: 2071i_S22

description:
2071i_S22
processed data file: rel_mill.txt

value: HFHS+PBS


title: LD_4

value: M


title: LD_2


title: LD_3


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C2_ad002


title: LD_1


title: non IgAN GN_22

source_name: non IgAN GN

value: non-IgAN glomerulonephritis (non-IgAN GN


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C12_ad008

value: SSs


title: 2070i_S21

description:
2070i_S21
processed data file: rel_mill.txt


title: non IgAN GN_21

value: m


title: LD_5


title: 2069i_S20

description:
2069i_S20
processed data file: rel_mill.txt


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C12_ad007

value: male


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C12_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C12_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C12_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C11_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C11_ad010


title: 1998i_S18

description:
1998i_S18
processed data file: rel_mill.txt

value: Experiment 1


title: 2066i_S19

description:
2066i_S19
processed data file: rel_mill.txt


title: 1997i_S17

description:
1997i_S17
processed data file: rel_mill.txt


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C11_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C11_ad006


title: non IgAN GN_19


title: 1996i_S16

description:
1996i_S16
processed data file: rel_mill.txt


title: non IgAN GN_18


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C11_ad004


title: non IgAN GN_20


title: non IgAN GN_16


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C11_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C10_ad008


title: 1995i_S15

description:
1995i_S15
processed data file: rel_mill.txt


title: 1993i_S14

description:
1993i_S14
processed data file: rel_mill.txt


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C10_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C10_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C11_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C10_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C11_ad008


title: 1992i_S13

description:
1992i_S13
processed data file: rel_mill.txt


title: 1991i_S12

description:
1991i_S12
processed data file: rel_mill.txt


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C10_ad002


title: non IgAN GN_13


title: non IgAN GN_14


title: non IgAN GN_15


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C1_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C1_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C1_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C1_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C10_ad004


title: 1989i_S10

description:
1989i_S10
processed data file: rel_mill.txt


title: non IgAN GN_12


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C1_ad002


title: 1988i_S9

description:
1988i_S9
processed data file: rel_mill.txt


title: 1987i_S8

description:
1987i_S8
processed data file: rel_mill.txt


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B9_ad001


title: non IgAN GN_11


title: non IgAN GN_9


title: 1990i_S11

description:
1990i_S11
processed data file: rel_mill.txt


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C1_ad008


title: 1986i_S7

description:
1986i_S7
processed data file: rel_mill.txt


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B9_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B9_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B9_ad007


title: 1985i_S6

description:
1985i_S6
processed data file: rel_mill.txt


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B8_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B8_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_C1_ad006


title: Bahcc1m/m2, NPCs, day 2, rep2

description: Bahcc1 m/m2 2 day 2

growth_protocol: R1 mouse ES cells were grown on a feeder layer of irradiated mouse embryonic fibroblasts (MEFs) on 0.1% gelatin-coated cell culture dishes. Culture medium consisted of DMEM (Gibco), 15% ES-grade fetal calf serum (FCS, Biological Industries), 1X Glutamax (Gibco), 1 mM sodium pyruvate (Gibco), 0.1 mM nonessential amino acids (Gibco), 0.1 mM β-mercaptoethanol (Sigma), 100 U/ml penicillin and 0.1 mg/ml streptomycin (Biological Industries), and 1000 U/ml LIF. Neuronal differentiation was performed as previously described (Ying et al., 2003). Pluripotent stem cells were first grown in the absence of MEFs for two passages and then seeded on gelatin-coated plates at a density of 2.1*104/cm2 in N2B27 medium: 1:1 mixture of DMEM/F12 (Sigma) supplemented with N2 (Gibco), and Neurobasal medium (Gibco) supplemented with B27 (Gibco), 1X Glutamax (Gibco) ), 0.1 mM β-mercaptoethanol (Sigma), 100 U/ml penicillin, and 0.1 mg/ml streptomycin (Biological industries). After four days under these conditions, the cells were dissociated and re-plated on plates coated with Poly-D-Lysine (Sigma) and Laminin (Sigma), at a density of 3.7*10^4/cm2 in N2B27 medium supplemented with 20ng/ml FGF2 (Peprotech). After 24 hr, FGF2 was removed from medium and cells were grown for three additional days.

extract_protocol:
ATAC-seq was performed as previously described (Buenrostro et al., 2013) with minor adjustment for application to tissue material. Six fresh L4-L6 DRGs were extracted in 100 µl ice cold cell lysis buffer (10mM Tris-HCl pH 7.4, 10mM NaCl, 3mM MgCl2, 0.5% IGEPAL CA-630) for 5 minutes on ice, then a 21g needle on a 1 mL syringe was used to shear the tissue through the needle 5 times.
As in Buenrstro et al. 2013

tag: cell type

value: NPCs

tag: genotype

value: WT

value: Bahcc1m/m2, day 2


title: non IgAN GN_10


title: non IgAN GN_7

value: F


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B8_ad008


title: non IgAN GN_6


title: non IgAN GN_8


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B8_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B8_ad006


title: Bahcc1m/m2, NPCs, day 2, rep1

description: Bahcc1 m/m2 1 day 2


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B8_ad002


title: non IgAN GN_17


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B8_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B7_ad012


title: non IgAN GN_5


title: Bahcc1m/m1, NPCs, day 2, rep2

description: Bahcc1 m/m1 2 day 2

value: Bahcc1m/m1, day 2


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B7_ad006


title: non IgAN GN_3


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B7_ad007


title: Renom/m2, NPCs, day 2, rep2

description: Reno m/m2 2 day 2

value: Renom/m2, day 2


title: Bahcc1m/m1, NPCs, day 2, rep1

description: Bahcc1 m/m1 1 day 2


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B7_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B7_ad008


title: Renom/m2, NPCs, day 2, rep1

description: Reno m/m2 1 day 2


title: non IgAN GN_4


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B6_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B6_ad001


title: non IgAN GN_2


title: Renom/m1, NPCs, day 2, rep1

description: Reno m/m1 1 day 2

value: Renom/m1, day 2


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B6_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B6_ad007


title: MIXED_lesions_IgAN_10

value: IgAN patient

tag: igan patient grpup


title: MIXED_lesions_IgAN_9


title: non IgAN GN_1


title: MIXED_lesions_IgAN_8


title: WT2, NPCs, day 2, rep2

description: WT2 2 day 2

value: WT2, day 2


title: Renom/m1, NPCs, day 2, rep2

description: Reno m/m1 2 day 2


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B6_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B6_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B6_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B7_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B5_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B5_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B5_ad004


title: WT2, NPCs, day 2, rep1

description: WT2 1 day 2


title: WT1, NPCs, day 2, rep2

description: WT1 2 day 2

value: WT1, day 2


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B5_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B5_ad001


title: WT1, NPCs, day 2, rep1

description: WT1 1 day 2


title: MIXED_lesions_IgAN_7

value: FFPE_Kidney_biopsy specimen


title: SW-1990_sh-Ctrl_biological rep1

description: Gene expression data from biological replicate 1 of SW1990 cells infected with scrambles shRNA

value: pancreas; Derived from metastatic site: spleen

value: scrambled shRNA


title: Bahcc1m/m2, NPCs, day 4, rep2

description: Bahcc1 m/m2 2

extract_protocol:
Total RNA was extracted from cells, using the Trizol reagent (TRI, Sigma-Aldrich) according to the manufacturer’s protocol.
TruSeq stranded mRNA kit

value: Bahcc1m/m2, day 4


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B5_ad008

value: SSs


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B5_ad002

value: male


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B4_ad012


title: Bahcc1m/m2, NPCs, day 4, rep1

description: Bahcc1 m/m2 1


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B4_ad007


title: MIXED_lesions_IgAN_6


title: MIXED_lesions_IgAN_3

value: M


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B4_ad010


title: MIXED_lesions_IgAN_4


title: MIXED_lesions_IgAN_5


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B4_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B4_ad002


title: Bahcc1m/m1, NPCs, day 4, rep1

description: Bahcc1 m/m1 1

value: NPCs

value: Bahcc1m/m1, day 4


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B4_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B3_ad001


title: Renom/m2, NPCs, day 4, rep2

description: Reno m/m2 2

value: WT

value: Renom/m2, day 4


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B3_ad012


title: MIXED_lesions_IgAN_2


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B3_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B4_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B3_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B3_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B3_ad004


title: Renom/m1, NPCs, day 4, rep2

description: Reno m/m1 2

value: Renom/m1, day 4


title: Bahcc1m/m1, NPCs, day 4, rep2

description: Bahcc1 m/m1 2


title: chronic_lesion_IgAN_11

value: F


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B2_ad012


title: MIXED_lesions_IgAN_1


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B2_ad001


title: WT2, NPCs, day 4, rep1

description: WT2 1

value: WT2, day 4


title: chronic_lesion_IgAN_9

value: IgAN patient


title: chronic_lesion_IgAN_10


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B2_ad007


title: chronic_lesion_IgAN_8

value: m


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B2_ad006


title: Renom/m2, NPCs, day 4, rep1

description: Reno m/m2 1


title: chronic_lesion_IgAN_7


title: Renom/m1, NPCs, day 4, rep1

description: Reno m/m1 1


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B2_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B2_ad010


title: WT2, NPCs, day 4, rep2

description: WT2 2


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B2_ad002


title: WT1, NPCs, day 4, rep2

description: WT1 2

value: WT1, day 4


title: chronic_lesion_IgAN_5

value: FFPE_Kidney_biopsy specimen


title: WT1, NPCs, day 4, rep1

description: WT1 1


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B12_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B12_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B12_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B12_ad012


title: chronic_lesion_IgAN_12


title: Cox10as1 shRNA1, neurons, day 8, rep2

description: Cox10as1 shRNA1 2

source_name: Neurons

value: Neurons

value: Cox10as1 shRNA1, day 8


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B2_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B11_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B12_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B12_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B11_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B12_ad001


title: Cox10as1 shRNA2, neurons, day 8, rep2

description: Cox10as1 shRNA2 2

value: Cox10as1 shRNA2, day 8


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B11_ad008

value: SSs


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B11_ad002

value: male


title: Cox10as1 shRNA2, neurons, day 8, rep1

description: Cox10as1 shRNA2 1


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B11_ad004


title: chronic_lesion_IgAN_6

value: f


title: chronic_lesion_IgAN_4

value: M


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B11_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B11_ad001


title: chronic_lesion_IgAN_2


title: chronic_lesion_IgAN_3


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B10_ad010


title: active_lesion_IgAN_6


title: Cox10as1 shRNA1, neurons, day 8, rep1

description: Cox10as1 shRNA1 1


title: lnc-Nr2f1 shRNA2, neurons, day 8, rep2

description: lnc-Nr2f1 shRNA2 2

value: lnc-Nr2f1 shRNA2, day 8


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B10_ad012


title: lnc-Nr2f1 shRNA1, neurons, day 8, rep2

description: lnc-Nr2f1 shRNA1 2

value: WT

value: lnc-Nr2f1 shRNA1, day 8


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B10_ad008


title: active_lesion_IgAN_8


title: lnc-Nr2f1 shRNA1, neurons, day 8, rep1

description: lnc-Nr2f1 shRNA1 1


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B10_ad007


title: active_lesion_IgAN_5


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B10_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B10_ad001


title: active_lesion_IgAN_7


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B1_ad012


title: Reno shRNA1, neurons, day 8, rep2

description: Reno shRNA1 2

value: Reno shRNA1, day 8


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B1_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B1_ad007


title: active_lesion_IgAN_3


title: active_lesion_IgAN_2


title: active_lesion_IgAN_4


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B1_ad004


title: Reno shRNA2, neurons, day 8, rep2

description: Reno shRNA2 2

value: Reno shRNA2, day 8


title: CEMBA181002_5C_5_CEMBA181002_5C_6_B1_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A9_ad012


title: Reno shRNA1, neurons, day 8, rep1

description: Reno shRNA1 1


title: lnc-Nr2f1 shRNA2, neurons, day 8, rep1

description: lnc-Nr2f1 shRNA2 1


title: NT shRNA, neurons, day 8, rep3

description: sh-NT neurons 3

value: NT shRNA, day 8


title: minimal_lesion_IgAN_22

value: IgAN patient


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A9_ad007


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A9_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A9_ad010


title: Reno shRNA2, neurons, day 8, rep1

description: Reno shRNA2 1


title: NT shRNA, neurons, day 8, rep2

description: sh-NT neurons 2


title: active_lesion_IgAN_1

value: F


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A9_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A9_ad004


title: Neurons, differentiation day8

description: Neurons

value: differentiation day8


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A9_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A9_ad002


title: chronic_lesion_IgAN_1

value: FFPE_Kidney_biopsy specimen


title: NPCs, differentiation day 4

description: NPCs

value: NPCs

value: differentiation day 4


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A8_ad008


title: minimal_lesion_IgAN_19


title: ES cells

description: ES cells

source_name: ES cells

value: ES cells


title: NT shRNA, neurons, day 8, rep1

description: sh-NT neurons 1

value: Neurons


title: minimal_lesion_IgAN_21


title: SSK41 CABIN1 KD IgM Stimulated replicate 2

city: Miami

first: Marco

last: Magistri

state: FL

address: 1501 NW 10th Ave

department: Psychiatry

institute: University of Miami

data_processing:
Paired-ends reads were trimmed off the adaptor sequences using a custom script.
Trimmed reads were aligned to the human genome reference hg19 using STAR aligner
Transcriptome for each sample was assembled using Cufflinks, which also provided transcripts abundance in fragments per kilobase of exon per million fragments mapped (FPKM)
Differential gene expression analysis was performed using DEseq2
Genome_build: hg19
Supplementary_files_format_and_content: Excel file including RPKM values for each Sample

source_name: B cell marginal zone lymphoma

growth_protocol: For cells stimulation, SSK41 cells were placed in serum free media and then stimulated with anti-IgM antibody

extract_protocol:
RNA was extracted using Trizol
1mg of RNA was used to prepare poly-A selected directional libraries using the KAPA Library Prep Kit

treatment_protocol: Cells were infected with lentivirus expressing either shRNA control or CABIN1-specific shRNA

tag: shRNA treatment

value: PLL3.7 CABIN1 shRNA

tag: stimulation

value: anti-IgM antibody


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A7_ad012

value: SSs

value: male


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A7_ad010


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A7_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A7_ad004


title: SSK41 CABIN1 KD IgM Stimulated replicate 3


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A7_ad007


title: minimal_lesion_IgAN_15


title: minimal_lesion_IgAN_18


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A7_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A8_ad012


title: minimal_lesion_IgAN_20

value: M


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A6_ad012


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A7_ad008


title: minimal_lesion_IgAN_16


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A7_ad002


title: SSK41 CABIN1 KD replicate 2


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A8_ad006


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A8_ad002


title: minimal_lesion_IgAN_13


title: minimal_lesion_IgAN_17


title: minimal_lesion_IgAN_12


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A6_ad007


title: minimal_lesion_IgAN_14


title: minimal_lesion_IgAN_11


title: SSK41 CABIN1 KD replicate 3


title: SSK41 CABIN1 KD replicate 1


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A6_ad006


title: SSK41 WT IgM Stimulated replicate 3

value: PLL3.7 scramble shRNA


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A6_ad008


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A5_ad001


title: minimal_lesion_IgAN_10


title: SSK41 WT replicate 3


title: SSK41 WT IgM Stimulated replicate 2


title: SSK41 WT IgM Stimulated replicate 1


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A6_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A5_ad002


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A5_ad010


title: SSK41 WT replicate 2


title: SSK41 CABIN1 KD IgM Stimulated replicate 1


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A6_ad001


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A5_ad008


title: minimal_lesion_IgAN_9


title: minimal_lesion_IgAN_8


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A4_ad007


title: SSK41 WT replicate 1


title: minimal_lesion_IgAN_7


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A4_ad010


title: minimal_lesion_IgAN_6


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A6_ad004


title: CEMBA181002_5C_5_CEMBA181002_5C_6_A5_ad007