title: TS139_12_74-3

status: Public on Oct 22 2020

city: Manchester

first: Tim

last: Somervaille

address: Wilmslow Rd

department: Manchester Institute

institute: Cancer Research UK

data_processing:
FASTQ files were aligned against the GRCh38/hg38 reference human genome using LAST after training the aligner with a subsample of 10,000 reads. Structural variant calling was performed using NanoSV (Stancu et al. 2017).
Genome_build: GRCh38
Supplementary_files_format_and_content: bigwig

source_name: Primary AML

growth_protocol: Cyropreserved primary cells were thawed and immediately used for DNA extraction

extract_protocol:
Genomic DNA was extracted from 5x106 cells using a QIAamp DNA mini kit (Qiagen, Manchester, UK).
1μg of genomic DNA was sheared with a g-TUBETM (Covaris, Inc., 520079) using 50μl sample volume and a 30s spin at 11,000rpm followed by inversion and a further 30s at 11,000rpm. Barcoded libraries were prepared from 45μl of fragmented DNA using a Ligation Sequencing Kit (Oxford Nanopore Technologies, SQK‐LSK108) according to a 1D Sequence Capture protocol with the following modifications: the pre-hybridisation PCR was replaced by a custom PCR with 6 cycles using Herculase II fusion DNA polymerase (Agilent). The subsequent AMPure XP purification was performed using a 0.6x bead to sample volume ratio. All purified product was used for the hybridization, which was performed using a SureSelect Target Enrichment System following the manufacturer’s protocol (https://www.agilent.com). The oligonucleotide library was designed using the online Agilent SureDesign service (https://earray.chem.agilent.com/suredesign/home.htm). The library targeted a 500kb region that included ABCB1 and upstream neighbouring genes (RUNDC3B, SLC25A40, DBF4 and ADAM22; chr7: 87,132,949-87,632,948 hg19). Following pull-down, the post-capture amplification was replaced by a custom PCR with 14 cycles as described above. Amplified libraries were quantified by Qubit (Invitrogen) and TapeStation 2200 (Agilent Technologies). An additional amplification was performed using 10 cycles of PCR and the product was quantified. Amplified libraries were again quantified by Qubit and 1μg of each library used for the End-prep. AMPure XP purification was performed using a 0.47x bead to sample ratio. Four end-prepped libraries were pooled into one adapter ligation reaction using 125ng of each, as measured by Tapestation. A 0.47x bead to sample ratio was used for the AMPure XP bead binding. Sequencing was performed on the MinION using the R9.4 Flowcell (Oxford Nanopore Technologies, FLO-MIN106) according to the manufacturer’s instructions. Nanopore sequencing of THP-1_R generated 0.92M reads with a mean read length of 1901bp and a maximum read length of 224.8kb. The coverage of the targeted region was 58.1x. Nanopore sequencing of relapsed AML generated an average of 1.28M reads per sample with a mean read length of 1484bp. Mean coverage of the targeted region was 45.3x.

value: Primary AML


title: TS139_16_191

type: SRA

middle: C

country: United Kingdom

library_source: genomic

molecule: genomic DNA

organism: Homo sapiens

tag: cell type


title: CD3+CD56- biological replicate 1

status: Public on Oct 23 2020

type: RNA

city: Naples

first: Mario

last: Galgani

address: via S. Pansini, 5

country: Italy

institute: IEOS Istituto per l'endocrinologia e l'oncologia sperimentale

description: Gene expression data from human lymphocytes

hyb_protocol: Hybridization is an integral part of the GeneChip Pico protocol (Cartridge Array Hybridization on the GeneChip¨ Instrument)

scan_protocol: The arrays were scanned on a GeneChip scanner 3000 7G

data_processing: Data was analyzed using the the Affymetrix Expression Console software. Normalization was done by RMA normalization

label: biotin

molecule: total RNA

source_name: CD3+CD56- lymphocyte subset flow sorted by PBMCs

label_protocol: The RNA was labeled using 2 ng and the GeneChip Pico Reagent kit and according to AffymetrixÕs protocol

growth_protocol: Cells were flow sorted and frozen immediately upon isolation

extract_protocol: The RNA was extracted according the RNNeasy Plus Micro Handbook 12/2014 and eluted using 20µl H2O

treatment_protocol: Cells were not treated in any way

value: T cell

tag: cell subset


title: TS139_15_THP1_pos_control


title: TS139_14_161-2


title: TS139_13_132


title: TS139_11_152


title: NK biological replicate 2

source_name: NK lymphocyte subset flow sorted by PBMCs

value: NK


title: NK biological replicate 3


title: CD8+ biological replicate 1

source_name: CD8+ lymphocyte subset flow sorted by PBMCs


title: CD3+CD56- biological replicate 3


title: TS139_09_162-2


title: TS139_10_105


title: TS139_08_99


title: TS139_07_124-11


title: TS139_06_149


title: TR3-56 biological replicate 3

source_name: TR3-56 lymphocyte subset flow sorted by PBMCs


title: Ctrl LSK cells Basal

city: BRONX

first: Shuxian

last: Dong

state: NY

address: 1300 morris park ave., Chanin 504

country: USA

institute: albert einstein college of medicine

description: gene expression data from Ctrl bone marrow LSK cells

hyb_protocol: 5.5 micrograms of cDNA from each sample was hybridized to ThermoFisher Scientific (Affymetrix) GeneChip Mouse Gene 2.0 ST Array (902118). Hybridization cocktail was made using the hybridization kit (ThermoFisher Scientific, 900454)

scan_protocol: array scans were performed according to the manufacturer's instructions using the high-resolution GeneArray Scanner 3000 7G (ThermoFisher Scientific, Santa Clara, CA,) (Affymetrix).

data_processing: The data were analyzed with the GeneChip Command Console Software from ThermoFisher Scientific (Affymetrix) default analysis settings.

organism: Mus musculus

source_name: LSK cells from Ctrl mice bone marrow at day0 after 5FU

label_protocol: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).

growth_protocol: LSK cells were freshly isolated from bone marrow by cell sorter acording to the surface marker (Lin-Scal+Ckit+) staining, cell pellete were flash frozen in liquid nitrogen and stored in -80 until RNA extraction

extract_protocol: Extraction was performed by RNeasy Micro kit (Qiagen) according to the manufacturer's protocols.

treatment_protocol: mice were injected with/without 150mg/kg 5FU and sacrificed at day8 after the injection.

tag: strain

tag: sample type

value: mice (male, 4m) bone marrow LSK


title: TS139_05_195


title: NK biological replicate 1


title: AMKO LSK cells 5FU

description: gene expression data from AMKO bone marrow LSK cells 8 days post 5FU injection

source_name: LSK cells from AMKO mice bone marrow at day8 after 5FU


title: TS139_03_128


title: TS139_04_246


title: TS139_01_110-9


title: TS139_02_165


title: TR3-56 biological replicate 1


title: TS96_8_380_T1


title: TS96_9_161_T30


title: TS77_THP1_R

growth_protocol: Cultured in RPMI 1640 medium (Sigma Aldrich) supplemented with 2mM L-Glutamine (Life Technologies) and 10% fetal bovine serum (Sigma Aldrich).

tag: cell line

tag: phenotype

value: Resistant


title: V_2

city: nanjing

first: liu

last: qingtao

state: jiangsu

address: zhongling road NO. 50

department: Institute of veterinary medicine

country: China

institute: jiangsu academy of agricultural sciences

description: miRNA.All.Counts.txt

library_source: transcriptomic

data_processing:
Reads filtering under criteria removing reads with 20% of the base quality lower than 13
Utilizing the BWA software, we mapped the clean data to miRBase 21.0 chicken miRNA database and chicken genome.
count counting
Genome_build: Gallus-5.0
Supplementary_files_format_and_content: TXT file contain counts for each sample

organism: Gallus gallus

source_name: H9N2 AIV infected chicken DCs

growth_protocol: bone marrow monocytes were cultured in 6-well plates at a concentration of 5 × 106/ml in RPMI-1640 (Wisent) complete medium containing 5% FBS (Wisent), 100 U/ml penicillin and 100 µg/ml streptomycin for 6 hours at 41°C in 5% CO2, and then non-adherent cells were removed by replacing with fresh complete medium containing 50 ng/ml chicken GM-CSF (Abcam, USA), and 10 ng/ml IL-4 (Kingfisher, USA). Half of the medium was replaced with pre-warmed complete medium containing GM-CSF and IL-4 on every second day until day 6. At day 7, BM-DCs were used for the infection of H9N2 AIV

extract_protocol:
Total RNA was extracted from cell or animal tissue by Trizol reagent (Invitrogen) separately. The RNA quality was checked by Bioanalyzer 2200 (Aligent) and kept at -80℃.The RNA with RIN >8.0 is right for cDNA library construction.
miRNA libraries were prepared for sequencing using standard Ion  Proton Protocols

treatment_protocol: The H9N2 influenza virus infected and mock infected cells were collected for RNA sequencing at 6 h post infection.

value: Bone marrow derived DCs

tag: infection

value: H9N2 AIV


title: V_3

description: miRNA.All.Counts.txt


title: TS96_497_T2


title: TR3-56 biological replicate 2


title: TS96_4_225_T15


title: TS96_54_T2


title: V_1

description: miRNA.All.Counts.txt


title: TS96_7_171_T3


title: M_1

description: miRNA.All.Counts.txt

source_name: Mock infected chicken DCs

value: Mock


title: TS96_3_53_T1


title: TS96_2_556_T2


title: TS96_5_14_T10


title: TS96_1_310_T2


title: M_3

description: miRNA.All.Counts.txt


title: TS96_10_338_T2


title: M_2

description: miRNA.All.Counts.txt


title: TS96_6_546_T6


title: si-SRSF2, rep2

city: Montpellier

first: Sylvie

last: Tuffery-Giraud

address: 641, av du Doyen Gaston Giraud

country: France

institute: Univ Montpellier

data_processing:
adapter sequences were removed with Cutadapt (v.1.3, default parameters)
quality-based trimming at the 3′ ends of reads was performed using the Qtrim tool (v.1.1, parameters: mean quality = 25, window size = 20, minimum read length = 40 nt)
Cleaned reads were mapped to the Human X chromosome reference sequence (hg19, UCSC) with STAR (v. 2.3, annotations from UCSC, parameters: -sjdbOverhang 29 -outFilterMismatchNoverLmax 0.05 -outSJfilterReads Unique, outFilterMultimapNmax 1)
Junctions were filtered to keep only those covered by a minimum of 5 reads in at least one out of the two biological replicates or 2/4 in the control condition.
The Percent-Spliced-In (PSI) was calculated for each splicing event using intron-centric metrics, with the SJPIPE pipeline, from the ipsa package (parameters: margin = 0, deltaSS = 0, mincount = 0, https://github.com/pervouchine/ipsa).
Genome_build: GRCh37 (hg19)
Supplementary_files_format_and_content: xlsx files include number of reads, psi5, psi3, cosi5, cosi3 values for each junction covered by a minimum of 5 reads in at least one out of the two biological replicates or 2/4 in the control condition.

source_name: human muscular cell line

growth_protocol: The C25cl48 cells were grown at 37C and 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) with GlutaMAX and 199 medium (4:1 ratio) (Life Technologies) supplemented with 20% calfserum (Eurobio), 5 ng/mL hEGF, 0.5 ng/ml bFGF, 50 µg/ml bovine fetuin (Life Technologies), 5 µg/ml Insulin and 0.2 µg/ml dexamethasone (Sigma–Aldrich). The differentiation for 3 days was induced by serum deprivation and elevation of insulin concentration to 10 μg/ml.

extract_protocol:
Total RNA was extracted using the RNeasy plus mini kit (Qiagen, Courtaboeuf, France). cDNA was prepared from 300 ng total RNA using 2.5 μM oligo dT and Superscript II (Thermo Fisher Scientific) as described by the manufacturer.The full-length DMD cDNA was amplified as a single long fragment (11.3 kb) by using the GoTaq Long PCR Master Mix (Promega, Charbonnières-les-Bains, France) and primers located in exon 1 (5′ -CTTTCCCCCTACAGGACTCAG-3′) and in the 3′ UTR (5′ -CCAAATCATCTGCCATGTGG-3′) of the muscle isoform (Dp427m) . LR-PCR reactions were purified using Qiaquick PCR purification kit (Qiagen) and quantified using the NanoDrop 2000 spectrophotometer (Thermo Fischer Scientific)
The library was prepared from 700 ng of purified PCR product fragmented by nebulization (2.1 bars of nitrogen for 1 min) and subjected to library preparation with the Rapid Library Preparation Kit (Roche) according to manufacturer’s instructions. The different samples were labeled via ligation of Multiplex Identifiers (MID) oligonucleotide adaptors to allow multiplexing. Three Multiplex Identifiers (MID)-tagged samples were pooled for simultaneous amplification and sequencing on the Roche GS Junior 454 sequencer.

treatment_protocol: Two consecutive siRNA transfections at a 48-h interval were performed. The first day C25Cl48 cells were seeded at 1.4.105 cells per well in six well-plates and reverse transfected with 37.5 nM of individual siRNA for all tested RBPs except CELF1, MBNL1 and QKI (20 nM) using 7.5 μl Lipofectamine RNAiMax (Thermo Fischer Scientific, Illkirch, France) following the manufacturer’s instructions. The differentiation was induced for three days from the day of second siRNA treatment, then cells were harvested for protein and RNA isolation.

tag: sirna-treatment


title: si-SRSF1, rep1


title: si-SRSF1, rep2


title: si-TDP-43, rep1


title: si-TRA2[beta], rep1


title: si-SRSF2, rep1


title: si-PTBP1, rep2


title: si-TRA2[beta], rep2


title: si-PTBP1, rep1


title: si-QKI, rep1

value: si-QKI


title: si-RBM4, rep1


title: si-TDP-43, rep2


title: si-hnRNPA2, rep1


title: si-MBNL1, rep2


title: si-hnRNPA1, rep2


title: si-RBFOX1, rep2


title: si-RBFOX1, rep1


title: si-DAZAP1, rep1


title: si-QKI, rep2


title: si-hnRNPA1, rep1


title: si-DDX5/DDX17, rep2


title: si-hnRNPA2, rep2


title: si-DDX5/DDX17, rep1


title: si-MBNL1, rep1


title: si-CELF1, rep2


title: si-RBM4, rep2


title: si-RBFOX2, rep1


title: si-RBFOX2, rep2


title: MEF transdifferentiated with OCT4 and SOD2

city: Bangalore

first: Genotypic

last: technology

state: Karnataka

address: 259, Apoorva 4th cross,80 feet Road,RMV 2ND STAGE

country: India

institute: Genotypic Technology

description: Gene expression after 6 passages

hyb_protocol: Fragmentation of labeled cRNA and hybridization were done using the Gene Expression Hybridization kit of (Agilent Technologies, In situ Hybridization kit, Part Number 5190-0404). Hybridization was carried out in Agilent’s Surehyb Chambers at 65º C for 16 hours. The hybridized slides were washed using Agilent Gene Expression wash buffers (Agilent Technologies, Part Number 5188-5327)

scan_protocol: Scanned using Agilent Microarray Scanner (Agilent Technologies, Part Number G2600D).

data_processing: Data extraction from Images was done using Feature Extraction software  and Normalization of the data was done in GeneSpring GX  : 75th percentile shift  method and gene based analysis was performed

source_name: MEFs transdfferentiated with OCT4 and SOD2

label_protocol: The samples for Gene expression were labeled using Agilent Quick-Amp labeling Kit (p/n5190-0442). The total RNA were reverse transcribed at 40°C using oligo dT primer tagged to a T7 polymerase promoter and converted to double stranded cDNA. Synthesized double stranded cDNA were used as template for cRNA generation. cRNA was generated by in vitro transcription and the dye Cy3 CTP(Agilent) was incorporated during this step. The cDNA synthesis and in vitro transcription steps were carried out at 40°C. Labeled cRNA was cleaned up using Qiagen RNeasy columns (Qiagen, Cat No: 74106) and quality assessed for yields and specific activity using the Nanodrop ND-1000.

growth_protocol: Neurospehere were grown in NSC media and transdifferentiated cells in NSC media

extract_protocol: RNA was prepared by using RNA isolation kit (Takara Bioscience) and total RNA was isolated according to the manufacturer’s protocol. RNA was quantified by Nanodrop (ThermoScientific Corporation)

treatment_protocol: MEF transduced with OCT4 and SOD2


title: Neural progenitor obtained from P2 mouse brain

description: Gene expression of neurosphere after 14 days in culture

source_name: primary neural progenitors

value: Neurosphere

value: P2 C57/Bl6 mice


title: si-control, rep2

value: si-control


title: si-control, rep1


title: si-control, rep4


title: si-control, rep3


title: si-DAZAP1, rep2


title: si-CELF1, rep1


title: NZM53

city: Dunedin

first: Aniruddha

last: Chatterjee

state: Otago

address: 270 Great king Street

department: Pathology

country: New Zealand

institute: University of Otago

description:
NZM53
53C_53T_DMAP.csv

data_processing:
DNA from 4 RRBS were sequenced in Illumina HiSeq Machines, Base-calling perfromed using RTA software and demultiplexed to obtain individual Fastq files.
Alignment was done using Bismark program.
UNIX scripts and our in house developed tool DMAP (Chatterjee et al 2012, Nucleic Acids Research) was used for post processing of the data
DNA methylation analysis was perfromed using a fragment based approach using our own in house developed pipeline called DMAP (Stockwell et al Bioinformatics, 2014).
Genome_build: GRCh37 (hg19)
Supplementary_files_format_and_content: .txt files describing methylation status of each significant MspI freagmnets (that were filtered based on high coverage)

source_name: Cell line

extract_protocol:
Cells were grown under standard conditions (5% CO2, 37°C, humidified atmosphere). : DNA from the cell pellets were extracted using Pure link Genomic DNA mini kit (Invitrogen, Hilden, Germany) following the manufacturer’s protocol, with the modification of proteinase K treatment being performed overnight at 55°C.
Genomic DNA was digested with MspI (New England Biolabs, Ipswich, MA) followed by end repair and addition of 30 A overhangs. Methylated adaptors (Illumina, San Diego, CA) with a 30 T overhang were then ligated with the generated fragments. Following adaptor ligation, DNA fragments ranging from 40 to 220 bp (preligation size) were cut from a 3% (w/v) NuSieve GTG agarose gel (Lonza, Basel, Switzerland) and subsequently bisulphite modified using the EZ DNA methylation kit (Zymo Research, Irvine, CA). The final library was amplified by PCR. The resulting library was sequenced on an Illumina platform with a single-ended, 100bp run.

tag: disease

value: melanoma


title: NZM60

description:
NZM60
60C_60T_FE_DMAP.csv


title: MC38 B+ replicate3

city: San Diego

first: Peng

last: Wu

state: CA

address: 10550 North Torrey Pines Road

department: Molecular Medicine

institute: Scripps Research

data_processing:
Library strategy: TCR-seq
Our data processing is a proprietary process in which reads are trimmed for quality, paired and stitched, then subjected to mapping to a build of the IMGT database for V-, D-, J-, and C-gene references.
With TCR data, following mapping and the identification of CDR3s via conserved motifs, additional filtering steps include the removal of chimeric sequences, sequences that likely contain PCR and/or sequencing error, and then a collapse of reads based on sequence identity (which forms the Frequency or Copy numbers seen in iRweb).
With TCR data, reads that have a frequency of 1 following collapse are removed.
The FASTQ files have not been trimmed nor stitched.  The FASTA files have only been trimmed for quality, overlapped, and stitched.  Each sample will have a R1 and R2 (FASTQ) and a FASTA formatted, trimmed and stitched read (3 files per sample).
Genome_build: We do not use a genome build per se, but use sequences pulled from the IMGT database
Supplementary_files_format_and_content: cvs files compressed in zip file contains TCRbeta sequences and their frequencies of each sample and statistic analysis

source_name: CD8 tumor infiltrating lymphocytes

extract_protocol:
Total RNA were extracted from 10000 cells per sample using PicopureTM RNA isolation kit (Qiagen RNase-Free DNase set were used to digest trace DNA).
TCRbeta library was prepared by SMART filter process (iRepertoire). Details please see: https://irepertoire.com/technology/
 Illumina MiSeq v2

treatment_protocol: Biotin+ and Biotin- CD8 TILs from murine E0771 and MC38 tumor were collected by FACS. Then TILs were cultured in T cell medium containing 100 IU/mL IL2 for 24 hours.

value: CD8 TILs from MC38

tag: replicate

tag: biotin

value: biotin+


title: MC38 B- replicate3

value: biotin-


title: MC38 B+ replicate2


title: 53T

description:
53T
53C_53T_DMAP.csv

source_name: Tissue


title: MC38 B- replicate2


title: MEF_NT

city: Grand Rapids

first: Gerd

middle: P.

last: Pfeifer

state: Michigan

address: 333 Bostwick Ave. NE.

department: Center for Epigenetics

institute: Van Andel Research Institute

data_processing:
Library strategy: circle-damage-seq
fastq files containing sequencing reads were hard-clipped from the 3’ end to 2x50 nucleotide length using Trim_Galore (https://www.bioinformatics.babraham.ac.uk/projects/trim_galore/).
Trimmed reads were aligned using BWA-MEM with default parameters.
Duplicate alignments were marked and removed with the ‘removeDups’ SAMBLASTER option.
SAMtools was then used to remove reads with low mapping quality (MAPQ <20), and to sort reads by genomic coordinates.
Divergently aligned read pairs (pairs facing away from each other) with a single nucleotide gap between reads were selected by selecting SAM records with TLEN of 3. TLEN equals the distance between the mapped end of the template and the mapped start of the template. Records with TLEN=3 represent divergently aligned reads with 1 nucleotide between mates.
Genome_build: mm10 for MEF and hg19 for THLE-2
Supplementary_files_format_and_content: bed files with a single nucleotide gap base

source_name: Mouse embryonic fibroblasts

growth_protocol: MEF cells were cultured in DMEM high glucose (Invitrogen; Carlsbad, CA) containing 15% fetal bovine serum and used at passage number <5. THLE-2 cells derived from primary human liver epithelial cells were obtained from ATCC (Cat. CRL-2706) and grown in BEGM medium (Lonza; Walkersville, MD) supplemented with 10% fetal bovine serum, 5 ng/ml EGF and 70 ng/ml phosphoethanolamine on the culture plates precoated with a mixture of 0.01 mg/ml fibronectin, 0.03 mg/ml collagen type I and 0.01 mg/ml BSA.

extract_protocol:
After the irradiation or chemical treatment, genomic DNA was isolated using Quick-DNA Miniprep Plus Kit (Zymo Research; Irvine, CA) according to the manufacturer’s instruction manual.
Genomic DNAs were framgmeted to an averge length of 300 bp and circuliarized with T4 DNA ligase at 16°C overnight. Non-circularized linear DNA were removed with Plasmid-Safe ATP-Dependent DNAse treatment. CPD lesions and etheno-dA adducts were cleaved with combined treatment of DNA glycosylases, APE1, AP lyase and S1 nuclease to create double-strand breaks with ligatable ends. DNA was then A-tailed with Klenow fragment exo- (NEB) and then adaptor was ligated with NEBNext Ultra II Ligation Master Mix and followed by incubation with USER enzyme (NEB). The purified DNA libraries were amplified by PCR with NEBNext Ultra II Q5 Master mix and NEBNext i5 and i7 primers and used for DNA sequencing.

treatment_protocol: To induce CPD adducts, MEF cells were irradiated with 1000 J/m2 dose of UVB. To induce 1,N6-etheno-deoxyadenosine (etheno-dA) adducts in human cells, the THLE-2 liver cells were treated with 40 μM of 4-HNE (Cayman; Ann Arbor, MI) in PBS for 10 min at 37°C.

value: MEF

tag: treatment

tag: repair time


title: MC38 B- replicate1


title: E0771 B- replicate1

value: CD8 TILs from E0771


title: MC38 B+ replicate1


title: E0771 B- replicate2


title: E0771 B+ replicate2


title: E0771 B+ replicate1


title: E0771 B- replicate3


title: 60T

description:
60T
60C_60T_FE_DMAP.csv


title: MEF_UVB

value: 1000 J/m2 of UVB

value: 0 hr


title: E0771 B+ replicate3


title: B6_F_T_16 [OxBS-seq]

city: Cambridge

first: Anne

last: Ferguson-Smith

address: Downing Site

department: Genetics

institute: University of Cambridge

description: re-analysis of GSM2480767

data_processing:
FastQ trimming: trim_galore v0.6.0 (parameters: --paired --retain_unpaired)
Mapping: bismark v0.20.0 (default parameters)
Mapping quality filtering: samtools v1.3.1 (parameters: -q 10)
Deduplication: deduplicate_bismark v0.20.0 (parameters: --bam --paired --multiple)
Methylation call extraction: bismark_methylation_extractor v0.20.0 (parameters: -p --ignore_r2 3 --ignore_3prime_r2 1 --merge_non_CpG)
Methylation call collation: collate_bismark_methy_calls and combine_bismark_stranded_methy (from github.com/knowah/bsseq-tools - version as at commit 1e1882e)
Genome_build: mm10 (GRCm38)
Supplementary_files_format_and_content: methylation call counts: Contains read count of methylation calls for each covered CpG. Calls from both strands are combined into one entry. Columns: chromosome, CpG position (+ strand cytosine), unmethylated reads, methylated reads

source_name: CD4+CD62L+ T cells

growth_protocol: C57BL/6 mice at 6-8 weeks of age were used for spleen collection purposes.

extract_protocol:
AllPrep DNA/RNA Mini Kit (Qiagen) was used to perform simultaneous isolation of RNA and DNA from 10^6 sorted naïve CD4+ T cells and resting B cells.
Triplicate 1µg samples of genomic DNA isolated from either naive CD4+ T cells or resting B cells were fragmented using a Covaris sonication system (Covaris S2). Following fragmentation, the DNA samples were spiked with synthetic fragments of DNA pre-labelled by PCR with 5mC and 5hmC used in the analysis step to determine the bisulfite conversion efficiency of cytosine forms (unmodified, 5mC and 5hmC). Libraries were prepared using the TruSeq DNA library preparation kit v2 (Illumina, FC-121-2001) according to manufacturer’s instructions with some modifications. After ligation of TruSeq methylated adapters (ME-100-0010, Illumina) and AMPure® XP (Beckman) bead purification half of the sample was oxidized by treatment with KRuO4 (15 mM in 0.05 M NaOH) for 1 hr, performed as previously described

treatment_protocol: Magnetic-activated cell sorting (MACS). For T cells: CD4+ CD62L+ T Cell Isolation Kit II (Miltenyi Biotec). For B cells: CD43-Ly48 CD4-L3T4 Ter-119 B Cell Isolation Kit (Miltenyi Biotec).

tag: Sex

value: female

value: CD4+CD62L+ T cells


title: B6_F_T_14 [OxBS-seq]

description: re-analysis of GSM2480766


title: B6_M_T_8 [OxBS-seq]

description: re-analysis of GSM2480771

value: male


title: B6_M_T_6 [OxBS-seq]

description: re-analysis of GSM2480770


title: B6_M_T_7 [BS-seq]

description: re-analysis of GSM2480763


title: B6_M_B_4 [BS-seq]

description: re-analysis of GSM2480761

source_name: CD43- B cells

value: CD43- B cells


title: B6_M_B_3 [OxBS-seq]

description: re-analysis of GSM2480768


title: B6_M_T_5 [BS-seq]

description: re-analysis of GSM2480762


title: B6_F_T_15 [BS-seq]

description: re-analysis of GSM2480759


title: B6_M_B_1 [BS-seq]

description: re-analysis of GSM2480760


title: B6_F_T_13 [BS-seq]

description: re-analysis of GSM2480758


title: B6_F_B_11 [OxBS-seq]

description: re-analysis of GSM2480765


title: B6_F_B_12 [BS-seq]

description: re-analysis of GSM2480756


title: B6_M_B_2 [OxBS-seq]

description: re-analysis of GSM2480769


title: B6_F_B_9 [OxBS-seq]

description: re-analysis of GSM2480764


title: B6_F_B_10 [BS-seq]

description: re-analysis of GSM2480757


title: KO MGE Replicate 1

city: San Francisco

first: Corey

last: Harwell

address: 35 Medical Center way

department: Neurology

institute: University of California, San Francisco

data_processing:
Sequence reads were trimmed to remove possible adapter sequences and nucleotides with poor quality using Trimmomatic v.0.36
 The trimmed reads were mapped to the Mus musculus GRCm38 reference genome available on ENSEMBL using the STAR aligner v.2.5.2b
Unique gene hit counts were calculated by using featureCounts from the Subread package v.1.5.2. The hit counts were summarized and reported using the gene_id feature in the annotation file. Only unique reads that fell within exon regions were counted.
After extraction of gene hit counts, the gene hit counts table was used for downstream differential expression analysis. Using DESeq2, a comparison of gene expression between the customer-defined groups of samples was performed. The Wald test was used to generate p-values and log2 fold changes. Genes with an adjusted p-value < 0.05 and absolute log2 fold change > 1 were called as differentially expressed genes for each comparison.
Genome_build:  Mus musculus GRCm38 reference genome
Supplementary_files_format_and_content: raw counts
Supplementary_files_format_and_content: normalized counts

source_name: medial ganglionic eminence from E14 embryonic brains

extract_protocol:
The MGEs from embryonic brains were dissected out and flash frozen in liquid nitrogen and pooled in sets of 3 embryos (6 MGEs) for total RNA extraction
RNA libraries were prepared for sequencing using standard Illumina protocols

tag: mouse line

value: Nkx2.1-Cre; Prdm16fl/fl; Ai14

tag: tissue

value: MGE

tag: developmental stage


title: KO MGE Replicate 2


title: WT MGE Replicate 2


title: WT MGE Replicate 3


title: PRDM16 input replicate 2

data_processing:
ChIP-Seq reads generated from PRDM16 ChIP and input libraries were aligned to the mouse genome using bowtie2 with default parameters except for “-I 50 -X 750”
The read densities were calculated with the Gaussian kernel (bandwidth of 35 bp) using the R package SPP
Peaks were called using MACS2 (version 2.1.2) with “callpeak” and a p-value threshold of 0.05 followed by Irreproducible Discovery Rate (IDR) analysis with a threshold of <= 0.05
Genome_build: MM10
Supplementary_files_format_and_content: BIGWIG

extract_protocol:
PRDM16 ChIP-seq was carried out using dual chromatin crosslinking with PFA and EGS, followed by sonication and antibody precipitation.
For library preparation, genomic DNA was purified, end repaired, ligated with barcoded adaptors, amplified for 14 PCR cycles and purified using the Ovation Ultralow System V2 (NuGEN). Library fragments in the range of 100-800 bp were size-selected using agarose gel electrophoresis followed by DNA gel extraction and purification.

tag: mouse strain

value: CD-1 (strain code 022, Charles River)


title: WT MGE Replicate 1


title: PRDM16 ChIP-Seq replicate 1


title: KO MGE Replicate 3


title: PRDM16 ChIP-Seq replicate 2


title: PRDM16 input replicate 1


title: S_7_KO_DBA

city: Madrid

first: Manuel

middle: J

last: Gomez

state: Madrid

address: Melchor Fernández Almagro, 3

country: Spain

institute: CNIC

description:
NormCounts.txt
B_S7

data_processing:
Base-calling was produced by Illumina RTA Software v 1.13.48
Demultiplexed fastq files were generated from the files using Piccard tools
Illumina adapter contaminations were removed from reads using Cutadapt.
Preprocessed reads were mapped and quantified on the transcriptome using RSEM.
Counts were normalized using Voom and TMM, from R packages Limma and EdgeR, respectively.
Genome_build: GRCm38 assembly, Ensembl genebuild 91.
Supplementary_files_format_and_content: NormCounts.txt fiile contains ENSEMBL gene identifiers, gene symbols and normalized counts per million for each sample.

molecule: polyA RNA

source_name: Spinal cord

growth_protocol: RNA-Seq experiments were conducted with 4 month-old male mice.

extract_protocol:
Total RNA was isolated from snap-frozen quadriceps muscle and spinal cord paired samples (from the same animal), preserved at -80C. Samples were homogenized in Trizol and total RNA was purified on-column using the PureLinkTM RNA Mini Kit (Invitrogen, Waltham, MA) according to manufacturer’s protocol and including an on-column DNase digest (PureLink™ DNase Set DNAse set, Invitrogen, Waltham, MA).  The quality of the extracted RNA was evaluated using the Agilent 2100 Bioanalyzer and the Eukaryote Total RNA Nano chip (Agilent, Santa Clara, CA).
cDNA libraries were prepared for sequencing using NEBNext Ultra RNA Library preparation kit (New England Biolabs).
Libraries were sequenced (single-end mode and length 61 bp) on the Illumina HiSeq2500 system.

treatment_protocol: Treated mice: neonate mice at postnatal day 1-2  were treated by intravenous injection in the temporal vein of AAV9-sp7-8-hGAAco, under the control of a liver-muscle promoter (LiMP), to provide for transgenic expression of human GAA.

tag: genotype

value: Gaa KO B6;129;DBA2/J (Ltbp4D36/D36)

value: untreated

tag: age

value: 4 months

tag: gender

value: Male

tag: sample pair


title: S_8_KO_DBA

description:
NormCounts.txt
B_S8


title: S_22_KO_DBA_treated

description:
NormCounts.txt
B_S25

value: AAV encoding secreted human GAA


title: S_21_KO_DBA_treated

description:
NormCounts.txt
B_S24


title: S_24_KO_DBA_treated

description:
NormCounts.txt
B_S24


title: S_4_WT_DBA

description:
NormCounts.txt
B_S4

value: Gaa WT B6;129;DBA2/J (Ltbp4D36/D36)


title: S_5_WT_B6;129

description:
NormCounts.txt
A_S5

value: Gaa WT B6;129


title: S_1_WT_DBA

description:
NormCounts.txt
B_S1


title: S_3_KO_B6;129

description:
NormCounts.txt
A_S3

value: Gaa KO B6;129


title: S_6_WT_B6;129

description:
NormCounts.txt
A_S6


title: S_3_WT_DBA

description:
NormCounts.txt
B_S3


title: S_4_WT_B6;129

description:
NormCounts.txt
A_S4


title: QUAD_8_KO_DBA

description:
NormCounts.txt
B_Q8

source_name: Quadriceps muscle


title: S_1_KO_B6;129

description:
NormCounts.txt
A_S1


title: QUAD_24_KO_DBA_treated

description:
NormCounts.txt
B_Q24


title: QUAD_21_KO_DBA_treated

description:
NormCounts.txt
B_Q21


title: QUAD_22_KO_DBA_treated

description:
NormCounts.txt
B_Q22


title: QUAD_7_KO_DBA

description:
NormCounts.txt
B_Q7


title: S_6_KO_DBA

description:
NormCounts.txt
B_S6


title: S_2_KO_B6;129

description:
NormCounts.txt
A_S2


title: QUAD_4_WT_DBA

description:
NormCounts.txt
B_Q4


title: QUAD_3_WT_DBA

description:
NormCounts.txt
B_Q3


title: QUAD_6_KO_DBA

description:
NormCounts.txt
B_Q6


title: QUAD_1_WT_DBA

description:
NormCounts.txt
B_Q1


title: QUAD_2_KO_B6;129

description:
NormCounts.txt
A_Q2


title: QUAD_1_KO_B6;129

description:
NormCounts.txt
A_Q1


title: QUAD_3_KO_B6;129

description:
NormCounts.txt
A_Q3


title: QUAD_5_WT_B6;129

description:
NormCounts.txt
A_Q5


title: QUAD_4_WT_B6;129

description:
NormCounts.txt
A_Q4


title: B3

city: Bar Harbor

first: Michael

middle: C.

last: Saul

state: ME

address: 600 Main St

institute: The Jackson Laboratory

data_processing:
The resulting sequence files were converted into FASTQ format and demultiplexed using CASAVA v1.8.2.
Raw FASTQ files were trimmed using Trimmomatic v0.35 running on Java v1.8.0_72 in single end mode.
Trimmed files were aligned the GRCm38 mouse genome with Ensembl v75 annotation.
Alignment was performed with Tophat2 v2.1.0, which utilized Bowtie2 v2.2.6 and Samtools v1.3.
Reads were counted using htseq-count from the HTSeq Python framework v0.6.0 in Python v2.7.10.
Genome_build: GRCm38 with Ensembl v75 annotation
Supplementary_files_format_and_content: Tab-delimited text files containing Ensembl ID and counts in gene features.

source_name: 3D fibrin derived tumor repopulating cells

growth_protocol: Growth protocol described in depth in publication.

extract_protocol:
Total RNA was extracted and purified using Qiagen’s RNeasy Mini Kit (Qiagen catalog #217004) according to manufacturer’s protocol.
Stranded RNA-seq libraries were prepared with Illumina's TruSeq Stranded RNA Sample Prep kit. The libraries were pooled in equimolar concentration and the pool was quantitated by qPCR and sequenced on two lanes for 101 cycles on a HiSeq 2500 using a TruSeq SBS sequencing kit v1.

treatment_protocol: Treatment protocol described in depth in publication.

value: TRC


title: B1


title: QUAD_6_WT_B6;129

description:
NormCounts.txt
A_Q6


title: A3

source_name: Parental B16F1 cells

value: parental


title: At_d5rf seedling RNA-seq

city: Wuhan

first: Shaojuan

last: Zhang

state: Hubei

address: Shizishan Street, Hongshan District

department: College of Life Sciences and Technology

institute: Huazhong Agricultural University

data_processing:
RNA degradation and contamination was monitored on 1% agarose gels
RNA purity was checked using the NanoPhotometer®spectrophotometer(IMPLEN, CA, USA) .
RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the  Bioanalyzer 2100 system (Agilent Technologies, CA, USA)
Library preparation for Transcriptome sequencing A total amount of 1 µg RNA per sample was used as input material for the RNA sample preparations.Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s  recommendations and index codes were added to attribute sequences to each sample. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer(5X). First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase(RNase H). Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H. Remaining overhangs were  converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3’ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure were ligated to prepare for hybridization. In order to select cDNA fragments of preferentially 250~300 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then 3 µl USER Enzyme (NEB, USA)  was used with size-selected, adaptor-ligated cDNA at 37°C for 15 min followed by 5 min at 95 °C before PCR. Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system.
Clustering and sequencing (Novogene Experimental Department).The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumia) according to the manufacturer’s instructions. After cluster generation, the library preparations were sequenced on an Illumina Novaseq platform and 150 bp paired-end reads were generated.
Genome_build: http://plants.ensembl.org/Arabidopsis_thaliana/Info/Index
Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every sample

organism: Arabidopsis thaliana

source_name: Arabidopsis thaliana RNA

growth_protocol: all Arabidopsis materials were grown under long photoperiod conditions (16 h light/8 h dark) at 22°C. Seedlings were grown on Murashige and Skoog plates containing 3% Sucrose and 0.8% agar or pots containing mixture of vermiculite and peat soil (1:1 by volume).

extract_protocol:
10 day old Arabidopsis seedlings were transferred to liquid nitrogen,and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of
RNA libraries were prepared for sequencing using standard Illumina protocols

value: whole seeding

value: d5rf T-DNA insertion mutant


title: At_col_0 seedling RNA-seq

value: wild type


title: A1


title: PGP1 hiPSCs, transduction 3, TF induction 3, TRA-1-60 high

city: Boston

first: Alex

last: Ng

state: MA

address: 77 Avenue Louis Pasteur Rm 233

institute: Harvard Medical School

data_processing:
bcl2fastq 2.17.1.14 was used for base-calling
STAR aligner v2.5.2a was used for read alignment
Log2(TRA-1-60low/TRA-1-60high) scores were computed using DESeq2
Genome_build: hg19 and synthesized TF sequences
Supplementary_files_format_and_content: Log2(Differentiated/Stem Cells) corresponds to Log2(TRA-1-60low/TRA-1-60high) from DESeq2

source_name: Induced pluripotent stem cells

growth_protocol: Human induced pluripotent stem cells were cultured on Matrigel in mTeSR1. Doxycycline was added for 4 days to induce TF expression.

extract_protocol:
Genomic DNA was extracted using QIAGEN DNeasy Blood & Tissue Kit. TFs were amplified by quantitative PCR using universal primers.
1 µg of PCR product per sample was sheared to an average of 200bp on a Covaris sonicator E220 and used for library preparation using NEBNext Ultra DNA Library Prep. Libraries were sequenced on the Illumina NextSeq on High Output Mode following the manufacturer's protocols.
DNA-seq

treatment_protocol: Cells were dissociated and stained using PE TRA-1-60 antibody then sorted into TRA-1-60 low versus TRA-1-60 high populations.

tag: cell line

tag: facs antibody

value: PE anti-TRA-1-60 (BD Biosciences, 560193)

value: TF induction using doxycycline

tag: facs gate

value: TRA-1-60 high


title: PGP1 hiPSCs, transduction 3, TF induction 5, TRA-1-60 high


title: Set 2, SOX9-induced oligodendrocytes

data_processing:
bcl2fastq 2.17.1.14 was used for base-calling
10x Chromium pipeline was used for alignment.
Count matrices were generated using CellRanger v2.0.0.
Genome_build: hg19
Supplementary_files_format_and_content: Gene-barcode matrix

extract_protocol:
RNA was extracted according to 10x Genomics manufacturer protocols.
scRNA-seq libraries were prepared according to 10x Genomics manufacturer protocols.

treatment_protocol: Cells were dissociated, counted and for set 1, samples were combined for loading into 10x Genomics chip.

tag: cell state

value: SOX9-Induced oligodendrocytes

value: TF-induction using doxycycline


title: PGP1 hiPSCs, transduction 3, TF induction 6, TRA-1-60 high


title: Set 2, Control hiPSCs

value: Pluripotent stem cells

value: No TF induction


title: PGP1 hiPSCs, transduction 3, TF induction 4, TRA-1-60 high


title: atcyp18-1_normal_2nd

city: Daejeon

first: Seung Hee

last: Jo

address: 125, Gwahakro

country: South Korea

institute: KRIBB

description: 7_0_2_1LIB030340_GEN00107337_S44_L002_R2.fastq

data_processing:
Raw sequencing data were filtered using standard RNA-seq parameters (Illumina pipeline).
The transcriptome short reads (<25 bp) were filtered out using DynamicTrim and LengthSort of solexaQA package
The read counts were calculated by mapping the filtered reads that passed the pre-processing using HTSeq
The filtered reads were normalised using the DESeq library
he IR ratio was calculated by dividing the read depth of introns by the average read depth of two adjacent exons
The intron retention (IR) ratio was calculated by dividing the read depth of introns by the average read depth of two adjacent exons. AS occurred outside of IRs requiring junction reads, junction information was extracted using regtools
Genome_build: Araport 11
Supplementary_files_format_and_content: Xlsx was processed data

source_name: Arabidopsis seed

growth_protocol: Seeds were surface-sterilised, stratified at 4℃ for 2 days, plated onto 1/2 MS agar medium, and transferred into a growth chamber.

extract_protocol:
Total RNA was prepared from seeds using Trizol (TaKaRa, Tyoko, Japan).
mRNA collectd from seeds was fragmented and used as a template for synthesis of cDNA using random hexamers and reverse transcriptase. Second-strand cDNA was synthesized using DNA polymerase I (New England BioLabs, Ipswich, MA, USA) amd RNase H (Invitrogen, Carlsbad, CA, USA). The resultant cDNA fragments were purifed, end-reparided, polyA-tailed, and ligated to index adapters following the Illumina protocol.

treatment_protocol: Heat stress (45℃) was applied to seeds at 1 day after imbibition

value: Seed

value: Germination


title: PGP1 hiPSCs, transduction 3, TF induction 2, TRA-1-60 high


title: WT_heat_1st

description: W_2_1_1LIB030340_GEN00107337_S44_L002_R2.fastq


title: atcyp18-1_heat_2nd

description: 7_2_2_1LIB030340_GEN00107337_S44_L002_R2.fastq


title: atcyp18-1_normal_3rd

description: 7_0_3_1LIB030340_GEN00107337_S44_L002_R2.fastq


title: atcyp18-1_heat_1st

description: 7_2_1_1LIB030340_GEN00107337_S44_L002_R2.fastq


title: WT_heat_2nd

description: W_2_2_1LIB030340_GEN00107337_S44_L002_R2.fastq


title: WT_heat_3rd

description: W_2_3_1LIB030340_GEN00107337_S44_L002_R2.fastq


title: WT_normal_3rd

description: W_0_3_1LIB030340_GEN00107337_S44_L002_R2.fastq


title: atcyp18-1_heat_3rd

description: 7_2_3_1LIB030340_GEN00107337_S44_L002_R2.fastq


title: WT_normal_1st

description: W_0_1_1LIB030340_GEN00107337_S44_L002_R2.fastq


title: PGP1 hiPSCs, transduction 2, TF induction 5, TRA-1-60 high


title: atcyp18-1_normal_1st

description: 7_0_1_1LIB030340_GEN00107337_S44_L002_R2.fastq


title: PGP1 hiPSCs, transduction 2, TF induction 4, TRA-1-60 high


title: PGP1 hiPSCs, transduction 2, TF induction 1, TRA-1-60 high


title: WT_normal_2nd

description: W_0_2_1LIB030340_GEN00107337_S44_L002_R2.fastq


title: PGP1 hiPSCs, transduction 2, TF induction 3, TRA-1-60 high


title: PGP1 hiPSCs, transduction 2, TF induction 2, TRA-1-60 high


title: PGP1 hiPSCs, transduction 1, TF induction 6, TRA-1-60 high


title: PGP1 hiPSCs, transduction 1, TF induction 5, TRA-1-60 high


title: PGP1 hiPSCs, transduction 1, TF induction 4, TRA-1-60 high


title: Set 1, Mixture of unmodified hiPSCs, ETV2-induced endothelial cells and control NKX3-2-induced fibroblasts

description: Cells were cultured separately, dissociated and mixed prior to scRNAseq

source_name: Induced pluripotent stem cells and derivatives

value: Mixture of pluripotent stem cells and TF-induced differentiated cells

value: Combination of uninduced and doxycycline-induced


title: PGP1 hiPSCs, transduction 3, TF induction 1, TRA-1-60 high


title: PGP1 hiPSCs, transduction 2, TF induction 6, TRA-1-60 high


title: PGP1 hiPSCs, transduction 1, TF induction 2, TRA-1-60 high


title: PGP1 hiPSCs, transduction 3, TF induction 5, TRA-1-60 low

value: TRA-1-60 low


title: PGP1 hiPSCs, transduction 1, TF induction 1, TRA-1-60 high


title: PGP1 hiPSCs, transduction 3, TF induction 4, TRA-1-60 low


title: PGP1 hiPSCs, transduction 1, TF induction 3, TRA-1-60 high


title: PGP1 hiPSCs, transduction 3, TF induction 3, TRA-1-60 low


title: PGP1 hiPSCs, transduction 2, TF induction 6, TRA-1-60 low


title: PGP1 hiPSCs, transduction 2, TF induction 2, TRA-1-60 low


title: PGP1 hiPSCs, transduction 3, TF induction 1, TRA-1-60 low


title: PGP1 hiPSCs, transduction 1, TF induction 2, TRA-1-60 low


title: PGP1 hiPSCs, transduction 1, TF induction 3, TRA-1-60 low


title: PGP1 hiPSCs, transduction 3, TF induction 6, TRA-1-60 low


title: PGP1 hiPSCs, transduction 2, TF induction 5, TRA-1-60 low


title: PGP1 hiPSCs, transduction 1, TF induction 6, TRA-1-60 low


title: PGP1 hiPSCs, transduction 1, TF induction 1, TRA-1-60 low


title: PGP1 hiPSCs, transduction 2, TF induction 3, TRA-1-60 low


title: PGP1 hiPSCs, transduction 1, TF induction 5, TRA-1-60 low


title: PGP1 hiPSCs, transduction 3, TF induction 2, TRA-1-60 low


title: PGP1 hiPSCs, transduction 2, TF induction 4, TRA-1-60 low


title: PGP1 hiPSCs, transduction 1, TF induction 4, TRA-1-60 low


title: PGP1 hiPSCs, transduction 2, TF induction 1, TRA-1-60 low


title: CRTD5 hiPSCs, transduction 3, TF induction 4, TRA-1-60 low


title: CRTD5 hiPSCs, transduction 3, TF induction 6, TRA-1-60 high


title: CRTD5 hiPSCs, transduction 3, TF induction 4, TRA-1-60 high


title: CRTD5 hiPSCs, transduction 3, TF induction 5, TRA-1-60 high


title: CRTD5 hiPSCs, transduction 3, TF induction 5, TRA-1-60 low


title: CRTD5 hiPSCs, transduction 3, TF induction 3, TRA-1-60 low


title: CRTD5 hiPSCs, transduction 3, TF induction 2, TRA-1-60 high


title: CRTD5 hiPSCs, transduction 3, TF induction 3, TRA-1-60 high


title: CRTD5 hiPSCs, transduction 3, TF induction 6, TRA-1-60 low


title: CRTD5 hiPSCs, transduction 3, TF induction 2, TRA-1-60 low


title: CRTD5 hiPSCs, transduction 3, TF induction 1, TRA-1-60 low


title: CRTD5 hiPSCs, transduction 2, TF induction 6, TRA-1-60 low


title: CRTD5 hiPSCs, transduction 3, TF induction 1, TRA-1-60 high


title: CRTD5 hiPSCs, transduction 2, TF induction 5, TRA-1-60 low


title: CRTD5 hiPSCs, transduction 2, TF induction 4, TRA-1-60 high


title: CRTD5 hiPSCs, transduction 2, TF induction 5, TRA-1-60 high


title: CRTD5 hiPSCs, transduction 2, TF induction 4, TRA-1-60 low


title: CRTD5 hiPSCs, transduction 1, TF induction 6, TRA-1-60 high


title: CRTD5 hiPSCs, transduction 2, TF induction 3, TRA-1-60 low


title: CRTD5 hiPSCs, transduction 2, TF induction 2, TRA-1-60 low


title: CRTD5 hiPSCs, transduction 2, TF induction 1, TRA-1-60 high


title: CRTD5 hiPSCs, transduction 2, TF induction 3, TRA-1-60 high


title: CRTD5 hiPSCs, transduction 2, TF induction 2, TRA-1-60 high


title: CRTD5 hiPSCs, transduction 2, TF induction 6, TRA-1-60 high


title: CRTD5 hiPSCs, transduction 1, TF induction 6, TRA-1-60 low


title: CRTD5 hiPSCs, transduction 1, TF induction 5, TRA-1-60 low


title: CRTD5 hiPSCs, transduction 1, TF induction 4, TRA-1-60 high


title: CRTD5 hiPSCs, transduction 1, TF induction 3, TRA-1-60 low


title: CRTD5 hiPSCs, transduction 1, TF induction 4, TRA-1-60 low


title: CRTD5 hiPSCs, transduction 1, TF induction 5, TRA-1-60 high


title: CRTD5 hiPSCs, transduction 1, TF induction 1, TRA-1-60 high


title: CRTD5 hiPSCs, transduction 2, TF induction 1, TRA-1-60 low


title: CRTD5 hiPSCs, transduction 1, TF induction 2, TRA-1-60 high


title: CRTD5 hiPSCs, transduction 1, TF induction 3, TRA-1-60 high


title: CRTD5 hiPSCs, transduction 1, TF induction 1, TRA-1-60 low


title: ATCC-DYS0100 hiPSCs, transduction 3, TF induction 5, TRA-1-60 high


title: ATCC-DYS0100 hiPSCs, transduction 2, TF induction 5, TRA-1-60 high


title: ATCC-DYS0100 hiPSCs, transduction 3, TF induction 2, TRA-1-60 high


title: ATCC-DYS0100 hiPSCs, transduction 3, TF induction 3, TRA-1-60 high


title: ATCC-DYS0100 hiPSCs, transduction 3, TF induction 1, TRA-1-60 high


title: ATCC-DYS0100 hiPSCs, transduction 3, TF induction 4, TRA-1-60 high


title: ATCC-DYS0100 hiPSCs, transduction 3, TF induction 6, TRA-1-60 high


title: ATCC-DYS0100 hiPSCs, transduction 2, TF induction 6, TRA-1-60 high


title: ATCC-DYS0100 hiPSCs, transduction 2, TF induction 3, TRA-1-60 high


title: ATCC-DYS0100 hiPSCs, transduction 2, TF induction 2, TRA-1-60 high


title: CRTD5 hiPSCs, transduction 1, TF induction 2, TRA-1-60 low


title: ATCC-DYS0100 hiPSCs, transduction 1, TF induction 5, TRA-1-60 high


title: ATCC-DYS0100 hiPSCs, transduction 1, TF induction 2, TRA-1-60 high


title: ATCC-DYS0100 hiPSCs, transduction 1, TF induction 6, TRA-1-60 high


title: ATCC-DYS0100 hiPSCs, transduction 1, TF induction 4, TRA-1-60 high


title: ATCC-DYS0100 hiPSCs, transduction 1, TF induction 3, TRA-1-60 high


title: ATCC-DYS0100 hiPSCs, transduction 1, TF induction 1, TRA-1-60 high


title: ATCC-DYS0100 hiPSCs, transduction 3, TF induction 6, TRA-1-60 low


title: ATCC-DYS0100 hiPSCs, transduction 2, TF induction 4, TRA-1-60 high


title: ATCC-DYS0100 hiPSCs, transduction 3, TF induction 5, TRA-1-60 low


title: ATCC-DYS0100 hiPSCs, transduction 2, TF induction 5, TRA-1-60 low


title: ATCC-DYS0100 hiPSCs, transduction 3, TF induction 1, TRA-1-60 low


title: ATCC-DYS0100 hiPSCs, transduction 3, TF induction 4, TRA-1-60 low


title: ATCC-DYS0100 hiPSCs, transduction 2, TF induction 4, TRA-1-60 low


title: ATCC-DYS0100 hiPSCs, transduction 2, TF induction 6, TRA-1-60 low


title: ATCC-DYS0100 hiPSCs, transduction 3, TF induction 2, TRA-1-60 low


title: ATCC-DYS0100 hiPSCs, transduction 2, TF induction 2, TRA-1-60 low


title: ATCC-DYS0100 hiPSCs, transduction 3, TF induction 3, TRA-1-60 low


title: ATCC-DYS0100 hiPSCs, transduction 2, TF induction 3, TRA-1-60 low


title: Set 3, SOX9-induced oligodendrocytes, replicate 3

data_processing:
bcl2fastq 2.17.1.14 was used for base-calling
STAR aligner v2.5.2a was used for read alignment
Log2 fold changes (dox/no dox) were computed using DESeq2
Genome_build: hg19
Supplementary_files_format_and_content: Expression matrix containing log2(dox/no dox) values

extract_protocol:
Total RNA was purified using the Direct-zol RNA Miniprep kit (Zymo Research).
1 µg RNA was used for Poly(A) isolation using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, E7490L) and polyA mRNA was preppared for sequencing using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, E7420L).

treatment_protocol: Cells were dissociated, lysed using TRIzol and stored at -20C.

value: SOX9-induced oligodendrocytes


title: ATCC-DYS0100 hiPSCs, transduction 1, TF induction 1, TRA-1-60 low


title: ATCC-DYS0100 hiPSCs, transduction 1, TF induction 5, TRA-1-60 low


title: ATCC-DYS0100 hiPSCs, transduction 1, TF induction 6, TRA-1-60 low


title: ATCC-DYS0100 hiPSCs, transduction 1, TF induction 4, TRA-1-60 low


title: ATCC-DYS0100 hiPSCs, transduction 1, TF induction 2, TRA-1-60 low


title: ATCC-DYS0100 hiPSCs, transduction 2, TF induction 1, TRA-1-60 low


title: Set 3, control hiPSCs, replicate 3


title: Set 3, control hiPSCs, replicate 2


title: Set 2, NKX3-1-induced fibroblasts, replicate 3

value: NKX3-1-induced fibroblasts


title: Set 3, control hiPSCs, replicate 1


title: Set 2, NKX3-1-induced fibroblasts, replicate 1


title: Set 2, control hiPSCs, replicate 2


title: Set 1, ETV2-induced vascular endothelial cells, replicate 3

value: ETV2-induced vascular endothelial cells


title: Set 1, ATOH-induced neurons, replicate 2

value: ATOH1-induced neurons


title: ATCC-DYS0100 hiPSCs, transduction 1, TF induction 3, TRA-1-60 low


title: Set 1, ATOH-induced neurons, replicate 1


title: Set 1, ETV2-induced vascular endothelial cells, replicate 2


title: Set 2, control hiPSCs, replicate 1


title: Set 1, control hiPSCs, replicate 2


title: Set 3, SOX9-induced oligodendrocytes, replicate 2


title: Set 1, control hiPSCs, replicate 3


title: Set 3, SOX9-induced oligodendrocytes, replicate 1


title: VZA01803D_P23

city: Solna

first: Vasilios

last: Zachariadis

address: Visionsgatan 4

department: Oncology-Pathology

country: Sweden

institute: Karolinska Institutet

data_processing:
Library strategy: DNTR-Seq
https://github.com/EngeLab/DNTRseq
Genome_build: hg38
Supplementary_files_format_and_content: mRNA read counts summarized over genes; DNA read counts summarized over genomic bins

source_name: DNTR-seq DNA library

growth_protocol: HCT116 and A375 cells were cultured in DMEM medium supplemented with 10% FBS and 1% Penicillin/strepotomycin at 37°C, 5% CO2 and humidified atmosphere.

extract_protocol:
Individual live cells were FACS-sorted into 384-well plates with lysis buffer, snap-frozen and stored at -80 degrees C.
After splitting each cell, separate mRNA- (*R suffix) and DNA-libraries (*D suffix) were constructed. As comparison, a set of cells had mRNA libraries prepared with Smart-Seq2 (no suffix).
Barcoded libraries were sequenced paired-end (37bp x 2) on an Illumina NextSeq.

treatment_protocol: Cells were untreated, treated with etoposide (ETO) or X-ray at increasing concentrations and harvested at different timepoints.

tag: cell id

tag: cell type

value: colorectal carcinoma

value: X-ray 3Gy

tag: time point

tag: cell count


title: Set 2, control hiPSCs, replicate 3

tag: treatment


title: VZA01803D_P24


title: VZA01803D_P22


title: VZA01803D_P21


title: Set 2, NKX3-1-induced fibroblasts, replicate 2

value: TF induction using doxycycline


title: VZA01803D_P17


title: VZA01803D_P20


title: VZA01803D_P14


title: VZA01803D_P18


title: VZA01803D_P15


title: VZA01803D_P16


title: VZA01803D_P11


title: Set 1, ETV2-induced vascular endothelial cells, replicate 1


title: ATCC-DYS0100 hiPSCs, transduction 2, TF induction 1, TRA-1-60 high

value: PE anti-TRA-1-60 (BD Biosciences, 560193)

value: TRA-1-60 high


title: Set 1, ATOH-induced neurons, replicate 3


title: VZA01803D_P05


title: VZA01803D_P09


title: VZA01803D_P08


title: VZA01803D_P10


title: VZA01803D_P19


title: VZA01803D_P03


title: VZA01803D_P04


title: VZA01803D_P02


title: VZA01803D_P01


title: VZA01803D_P13


title: VZA01803D_O20


title: VZA01803D_O14


title: VZA01803D_O19


title: VZA01803D_O24


title: VZA01803D_O18


title: VZA01803D_O16


title: VZA01803D_O22


title: VZA01803D_P12


title: VZA01803D_O13


title: VZA01803D_O17


title: VZA01803D_O12


title: VZA01803D_O15


title: VZA01803D_O11


title: VZA01803D_O04


title: VZA01803D_O05


title: VZA01803D_O10


title: VZA01803D_O06


title: VZA01803D_O09


title: VZA01803D_O21


title: VZA01803D_O23


title: VZA01803D_O02


title: VZA01803D_O08


title: VZA01803D_P07


title: VZA01803D_O03


title: VZA01803D_O01


title: VZA01803D_N24


title: VZA01803D_N21


title: VZA01803D_N23


title: VZA01803D_N18


title: VZA01803D_N19


title: VZA01803D_N16


title: VZA01803D_N20


title: VZA01803D_O07


title: VZA01803D_N22


title: VZA01803D_N14


title: VZA01803D_N15


title: VZA01803D_N12


title: VZA01803D_N09


title: VZA01803D_N10


title: VZA01803D_N13


title: VZA01803D_N17


title: VZA01803D_N06


title: VZA01803D_N05


title: VZA01803D_N04


title: VZA01803D_N11


title: VZA01803D_N07


title: VZA01803D_N01


title: VZA01803D_M23


title: VZA01803D_M24


title: VZA01803D_N03


title: VZA01803D_N02


title: VZA01803D_N08


title: VZA01803D_M22


title: VZA01803D_M21


title: VZA01803D_M15


title: VZA01803D_M16


title: VZA01803D_M18


title: VZA01803D_M13


title: VZA01803D_M14


title: VZA01803D_M09


title: VZA01803D_M11


title: VZA01803D_M17


title: VZA01803D_M07


title: VZA01803D_M06


title: VZA01803D_M19


title: VZA01803D_M12


title: VZA01803D_M04


title: VZA01803D_M05


title: VZA01803D_M10


title: VZA01803D_L23


title: VZA01803D_M02


title: VZA01803D_L22


title: VZA01803D_L21


title: VZA01803D_L20


title: VZA01803D_M01


title: VZA01803D_L19


title: VZA01803D_M03


title: VZA01803D_M20


title: VZA01803D_L17


title: VZA01803D_L24


title: Set 1, control hiPSCs, replicate 1


title: VZA01803D_M08


title: VZA01803D_L16


title: VZA01803D_L12


title: VZA01803D_L15


title: VZA01803D_L10


title: VZA01803D_L11


title: VZA01803D_L08


title: VZA01803D_L13


title: VZA01803D_L07


title: VZA01803D_L04


title: VZA01803D_L06


title: VZA01803D_L05


title: VZA01803D_L18


title: VZA01803D_L03


title: VZA01803D_K24


title: VZA01803D_K19


title: VZA01803D_K22


title: VZA01803D_L14


title: VZA01803D_L01


title: VZA01803D_K21


title: VZA01803D_K20


title: VZA01803D_K18


title: VZA01803D_L09


title: VZA01803D_K17


title: VZA01803D_K16


title: VZA01803D_K15


title: VZA01803D_K14


title: VZA01803D_K23


title: VZA01803D_K13


title: VZA01803D_K12


title: VZA01803D_K09


title: VZA01803D_K11


title: VZA01803D_K07


title: VZA01803D_L02


title: VZA01803D_K03


title: VZA01803D_K08


title: VZA01803D_K01


title: VZA01803D_K04


title: VZA01803D_J24


title: VZA01803D_K10


title: VZA01803D_J23


title: VZA01803D_K02


title: VZA01803D_K05


title: VZA01803D_J22


title: VZA01803D_J21


title: VZA01803D_J16


title: VZA01803D_J20


title: VZA01803D_J18


title: VZA01803D_J14


title: VZA01803D_J12


title: VZA01803D_J13


title: VZA01803D_J11


title: VZA01803D_J09


title: VZA01803D_J19


title: VZA01803D_J08


title: VZA01803D_J10


title: VZA01803D_K06


title: VZA01803D_J07


title: VZA01803D_J06


title: VZA01803D_P06


title: VZA01803D_J01


title: VZA01803D_I24


title: VZA01803D_J02


title: VZA01803D_J03


title: VZA01803D_J15


title: VZA01803D_I17


title: VZA01803D_I21


title: VZA01803D_I20


title: VZA01803D_J04


title: VZA01803D_I23


title: VZA01803D_I13


title: VZA01803D_I16


title: VZA01803D_I15


title: VZA01803D_I11


title: VZA01803D_I09


title: VZA01803D_I10


title: VZA01803D_I07


title: VZA01803D_I06


title: VZA01803D_I12


title: VZA01803D_I08


title: VZA01803D_I05


title: VZA01803D_I04


title: VZA01803D_I22


title: VZA01803D_I02


title: VZA01803D_I03


title: VZA01803D_I18


title: VZA01803D_I14


title: VZA01803D_H23


title: VZA01803D_I19


title: VZA01803D_H24


title: VZA01803D_I01


title: VZA01803D_H22


title: VZA01803D_H16


title: VZA01803D_H18


title: VZA01803D_H17


title: VZA01803D_H20


title: VZA01803D_H15


title: VZA01803D_H21


title: VZA01803D_H19


title: VZA01803D_H11


title: VZA01803D_H09


title: VZA01803D_H07


title: VZA01803D_H10


title: VZA01803D_H08


title: VZA01803D_H05


title: VZA01803D_H06


title: VZA01803D_H04


title: VZA01803D_H01


title: VZA01803D_H02


title: VZA01803D_G24


title: VZA01803D_H03


title: VZA01803D_G23


title: VZA01803D_G22


title: VZA01803D_J17


title: VZA01803D_G20


title: VZA01803D_G19


title: VZA01803D_H13


title: VZA01803D_G13


title: VZA01803D_H12


title: VZA01803D_H14


title: VZA01803D_G14


title: VZA01803D_G17


title: VZA01803D_G18


title: VZA01803D_J05


title: VZA01803D_G12


title: VZA01803D_G10


title: VZA01803D_G09


title: VZA01803D_G08


title: VZA01803D_G16


title: VZA01803D_G06


title: VZA01803D_G07


title: VZA01803D_G04


title: VZA01803D_G05


title: VZA01803D_G03


title: VZA01803D_G01


title: VZA01803D_G02


title: VZA01803D_G15


title: VZA01803D_F23


title: VZA01803D_F24


title: VZA01803D_F22


title: VZA01803D_F20


title: VZA01803D_F21


title: VZA01803D_G21


title: VZA01803D_F19


title: VZA01803D_F18


title: VZA01803D_F16


title: VZA01803D_G11


title: VZA01803D_F17


title: VZA01803D_F12


title: VZA01803D_F14


title: VZA01803D_F15


title: VZA01803D_F11


title: VZA01803D_F07


title: VZA01803D_F08


title: VZA01803D_F09


title: VZA01803D_F10


title: VZA01803D_F04


title: VZA01803D_F03


title: VZA01803D_F01


title: VZA01803D_F02


title: VZA01803D_E21


title: VZA01803D_E20


title: VZA01803D_E19


title: VZA01803D_E22


title: VZA01803D_E23


title: VZA01803D_E24


title: VZA01803D_E16


title: VZA01803D_E17


title: VZA01803D_E13


title: VZA01803D_E12


title: VZA01803D_F13


title: VZA01803D_E15


title: VZA01803D_E14


title: VZA01803D_E18


title: VZA01803D_E10


title: VZA01803D_E11


title: VZA01803D_E06


title: VZA01803D_E04


title: VZA01803D_E07


title: VZA01803D_F05


title: VZA01803D_E08


title: VZA01803D_F06


title: VZA01803D_E03


title: VZA01803D_E01


title: VZA01803D_E02


title: VZA01803D_D21


title: VZA01803D_D23


title: VZA01803D_D20


title: VZA01803D_D19


title: VZA01803D_D18


title: VZA01803D_D16


title: VZA01803D_D17


title: VZA01803D_D13


title: VZA01803D_D14


title: VZA01803D_D11


title: VZA01803D_D12


title: VZA01803D_E09


title: VZA01803D_D10


title: VZA01803D_D08


title: VZA01803D_D06


title: VZA01803D_E05


title: VZA01803D_D07


title: VZA01803D_D05


title: VZA01803D_D02


title: VZA01803D_D01


title: VZA01803D_D04


title: VZA01803D_D22


title: VZA01803D_C23


title: VZA01803D_C24


title: VZA01803D_D24


title: VZA01803D_C21


title: VZA01803D_C22


title: VZA01803D_D15


title: VZA01803D_C18


title: VZA01803D_C20


title: VZA01803D_C19


title: VZA01803D_D09


title: VZA01803D_C14


title: VZA01803D_C17


title: VZA01803D_C16


title: VZA01803D_C13


title: VZA01803D_C12


title: VZA01803D_C10


title: VZA01803D_C11


title: VZA01803D_C09


title: VZA01803D_C07


title: VZA01803D_C08


title: VZA01803D_C06


title: VZA01803D_D03


title: VZA01803D_C05


title: VZA01803D_C04


title: VZA01803D_C02


title: VZA01803D_B24


title: VZA01803D_C01


title: VZA01803D_B20


title: VZA01803D_B21


title: VZA01803D_B19


title: VZA01803D_B17


title: VZA01803D_B22


title: VZA01803D_B15


title: VZA01803D_C15


title: VZA01803D_B16


title: VZA01803D_B23


title: VZA01803D_B14


title: VZA01803D_B13


title: VZA01803D_B12


title: VZA01803D_B11


title: VZA01803D_B09


title: VZA01803D_C03


title: VZA01803D_B06


title: VZA01803D_B07


title: VZA01803D_B08


title: VZA01803D_B05


title: VZA01803D_B02


title: VZA01803D_B04


title: VZA01803D_B03


title: VZA01803D_B01


title: VZA01803D_A23


title: VZA01803D_A24


title: VZA01803D_B18


title: VZA01803D_A22


title: VZA01803D_A21


title: VZA01803D_A20


title: VZA01803D_A15


title: VZA01803D_A17


title: VZA01803D_A18


title: VZA01803D_B10


title: VZA01803D_A14


title: VZA01803D_A12


title: VZA01803D_A16


title: VZA01803D_A07


title: VZA01803D_A11


title: VZA01803D_A13


title: VZA01803D_A06


title: VZA01803D_A09


title: VZA01803D_A05


title: VZA01801D_P24

value: X-ray 5Gy


title: VZA01801D_P22


title: VZA01803D_A01


title: VZA01803D_A02


title: VZA01801D_P23


title: VZA01803D_A04


title: VZA01803D_A10


title: VZA01801D_P21


title: VZA01801D_P19


title: VZA01801D_P20


title: VZA01801D_P18


title: VZA01801D_P17


title: VZA01801D_P14


title: VZA01801D_P15


title: VZA01801D_P13


title: VZA01801D_P11


title: VZA01803D_A08


title: VZA01801D_P09


title: VZA01801D_P10


title: VZA01801D_P12


title: VZA01803D_A03


title: VZA01801D_P07

value: X-ray 1Gy


title: VZA01801D_P08


title: VZA01801D_P06


title: VZA01801D_P03


title: VZA01801D_P05


title: VZA01801D_P02


title: VZA01801D_O24


title: VZA01801D_O20


title: VZA01801D_O23


title: VZA01801D_O21


title: VZA01803D_A19


title: VZA01801D_O22


title: VZA01801D_O16


title: VZA01801D_O19


title: VZA01801D_O17


title: VZA01801D_O18


title: VZA01801D_O15


title: VZA01801D_P16


title: VZA01801D_P01


title: VZA01801D_O07


title: VZA01801D_O10


title: VZA01801D_O08


title: VZA01801D_O14


title: VZA01801D_O06


title: VZA01801D_O12


title: VZA01801D_O13


title: VZA01801D_O11


title: VZA01801D_O09


title: VZA01801D_P04


title: VZA01801D_O05


title: VZA01801D_O01


title: VZA01801D_O02


title: VZA01801D_O04


title: VZA01801D_N24


title: VZA01801D_N23


title: VZA01801D_N22


title: VZA01801D_N16


title: VZA01801D_N18


title: VZA01801D_N20


title: VZA01801D_N11


title: VZA01801D_N19


title: VZA01801D_N17


title: VZA01801D_N12


title: VZA01801D_N15


title: VZA01801D_N13


title: VZA01801D_N10


title: VZA01801D_N07


title: VZA01801D_N06


title: VZA01801D_N08


title: VZA01801D_N02


title: VZA01801D_M24


title: VZA01801D_N03


title: VZA01801D_N01


title: VZA01801D_M23


title: VZA01801D_M22


title: VZA01801D_M18


title: VZA01801D_M21


title: VZA01801D_M19


title: VZA01801D_O03


title: VZA01801D_N05


title: VZA01801D_M14


title: VZA01801D_M16


title: VZA01801D_M17


title: VZA01801D_M15


title: VZA01801D_N04


title: VZA01801D_N14


title: VZA01801D_N09


title: VZA01801D_M11


title: VZA01801D_M10


title: VZA01801D_M13


title: VZA01801D_M05


title: VZA01801D_M07


title: VZA01801D_M08


title: VZA01801D_M04


title: VZA01801D_M02


title: VZA01801D_M03


title: VZA01801D_L24


title: VZA01801D_L23


title: VZA01801D_L20


title: VZA01801D_L21


title: VZA01801D_L19


title: VZA01801D_L22


title: VZA01801D_L18


title: VZA01801D_L15


title: VZA01801D_L13


title: VZA01801D_L12


title: VZA01801D_L14


title: VZA01801D_L07


title: VZA01801D_L17


title: VZA01801D_L09


title: VZA01801D_M09


title: VZA01801D_L16


title: VZA01801D_L10


title: VZA01801D_L08


title: VZA01801D_L11


title: VZA01801D_M01


title: VZA01801D_L06


title: VZA01801D_L05


title: VZA01801D_M06


title: VZA01801D_L03


title: VZA01801D_L02


title: VZA01801D_K21


title: VZA01801D_K19


title: VZA01801D_K22


title: VZA01801D_L04


title: VZA01801D_K23


title: VZA01801D_N21


title: VZA01801D_K18


title: VZA01801D_L01


title: VZA01801D_K24


title: VZA01801D_K20


title: VZA01801D_K15


title: VZA01801D_K16


title: VZA01801D_K13


title: VZA01801D_K10


title: VZA01801D_K12


title: VZA01801D_K09


title: VZA01801D_K08


title: VZA01801D_K03


title: VZA01801D_K07


title: VZA01801D_K05


title: VZA01801D_K01


title: VZA01801D_J24


title: VZA01801D_K06


title: VZA01801D_K02


title: VZA01801D_J23


title: VZA01801D_J22


title: VZA01801D_K04


title: VZA01801D_J21


title: VZA01801D_K17


title: VZA01801D_J17


title: VZA01801D_K14


title: VZA01801D_J19


title: VZA01801D_J20


title: VZA01801D_J14


title: VZA01801D_J18


title: VZA01801D_J15


title: VZA01801D_K11


title: VZA01801D_J12


title: VZA01801D_J11


title: VZA01801D_J08


title: VZA01801D_J10


title: VZA01801D_J09


title: VZA01801D_J07


title: VZA01801D_J04


title: VZA01801D_J03


title: VZA01801D_J05


title: VZA01801D_J02


title: VZA01801D_J01


title: VZA01801D_I24


title: VZA01801D_I23


title: VZA01801D_I21


title: VZA01801D_I22


title: VZA01801D_I20


title: VZA01801D_M20


title: VZA01801D_J16


title: VZA01801D_J13


title: VZA01801D_I19


title: VZA01801D_I15


title: VZA01801D_I14


title: VZA01801D_I13


title: VZA01801D_I12


title: VZA01801D_I16


title: VZA01801D_I17


title: VZA01801D_I09


title: VZA01801D_I18


title: VZA01801D_I11


title: VZA01801D_I08


title: VZA01801D_I10


title: VZA01801D_J06


title: VZA01801D_I04


title: VZA01801D_I03


title: VZA01801D_I07


title: VZA01801D_I06


title: VZA01801D_I01


title: VZA01801D_I05


title: VZA01801D_I02


title: VZA01801D_H24


title: VZA01801D_H22


title: VZA01801D_H23


title: VZA01801D_H20


title: VZA01801D_H19


title: VZA01801D_H17


title: VZA01801D_H15


title: VZA01801D_H16


title: VZA01801D_H13


title: VZA01801D_H12


title: VZA01801D_H14


title: VZA01801D_H11


title: VZA01801D_H09


title: VZA01801D_H10


title: VZA01801D_H08


title: VZA01801D_H06


title: VZA01801D_H07


title: VZA01801D_H04


title: VZA01801D_H03


title: VZA01801D_H01


title: VZA01801D_H02


title: VZA01801D_H21


title: VZA01801D_G21


title: VZA01801D_G22


title: VZA01801D_G24


title: VZA01801D_H18


title: VZA01801D_G20


title: VZA01801D_G18


title: VZA01801D_G19


title: VZA01801D_G14


title: VZA01801D_G13


title: VZA01801D_G17


title: VZA01801D_G12


title: VZA01801D_G11


title: VZA01801D_G10


title: VZA01801D_G09


title: VZA01801D_M12


title: VZA01801D_H05


title: VZA01801D_G08


title: VZA01801D_G07


title: VZA01801D_G06


title: VZA01801D_G05


title: VZA01801D_G04


title: VZA01801D_G03


title: VZA01801D_G23


title: VZA01801D_G02


title: VZA01801D_F24


title: VZA01801D_F23


title: VZA01801D_G01


title: VZA01801D_F22


title: VZA01801D_F20


title: VZA01801D_F21


title: VZA01801D_F19


title: VZA01801D_F17


title: VZA01801D_G16


title: VZA01801D_G15


title: VZA01801D_F18


title: VZA01801D_F13


title: VZA01801D_F14


title: VZA01801D_F11


title: VZA01801D_F12


title: VZA01801D_F06


title: VZA01801D_F05


title: VZA01801D_F04


title: VZA01801D_F02


title: VZA01801D_F01


title: VZA01801D_E23


title: VZA01801D_F03


title: VZA01801D_E18


title: VZA01801D_E17


title: VZA01801D_F16


title: VZA01801D_E19


title: VZA01801D_E20


title: VZA01801D_E15


title: VZA01801D_E16


title: VZA01801D_F15


title: VZA01801D_E11


title: VZA01801D_F10


title: VZA01801D_F09


title: VZA01801D_E13


title: VZA01801D_F08


title: VZA01801D_E12


title: VZA01801D_E09


title: VZA01801D_F07


title: VZA01801D_E10


title: VZA01801D_E08


title: VZA01801D_E07


title: VZA01801D_E06


title: VZA01801D_E04


title: VZA01801D_E05


title: VZA01801D_E24


title: VZA01801D_E22


title: VZA01801D_E21


title: VZA01801D_E03


title: VZA01801D_E02


title: VZA01801D_E14


title: VZA01801D_D20


title: VZA01801D_D23


title: VZA01801D_D21


title: VZA01801D_D24


title: VZA01801D_D19


title: VZA01801D_E01


title: VZA01801D_D22


title: VZA01801D_D18


title: VZA01801D_D15


title: VZA01801D_D17


title: VZA01801D_D14


title: VZA01801D_D16


title: VZA01801D_D13


title: VZA01801D_D12


title: VZA01801D_D11


title: VZA01801D_D09


title: VZA01801D_D10


title: VZA01801D_D05


title: VZA01801D_D08


title: VZA01801D_D07


title: VZA01801D_D06


title: VZA01801D_D04


title: VZA01801D_D03


title: VZA01801D_D01


title: VZA01801D_C24


title: VZA01801D_C23


title: VZA01801D_C21


title: VZA01801D_C20


title: VZA01801D_C19


title: VZA01801D_C17


title: VZA01801D_C15


title: VZA01801D_C18


title: VZA01801D_C14


title: VZA01801D_C12


title: VZA01801D_C11


title: VZA01801D_C10


title: VZA01801D_C09


title: VZA01801D_C06


title: VZA01801D_C08


title: VZA01801D_C07


title: VZA01801D_C03


title: VZA01801D_C05


title: VZA01801D_C02


title: VZA01801D_C04


title: VZA01801D_D02


title: VZA01801D_C01


title: VZA01801D_C22


title: VZA01801D_B24


title: VZA01801D_B21


title: VZA01801D_B23


title: VZA01801D_B19


title: VZA01801D_B20


title: VZA01801D_B18


title: VZA01801D_B22


title: VZA01801D_C16


title: VZA01801D_B16


title: VZA01801D_B15


title: VZA01801D_C13


title: VZA01801D_B14


title: VZA01801D_B12


title: VZA01801D_B13


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title: VZA01801D_B11


title: VZA01801D_B07


title: VZA01801D_B06


title: VZA01801D_B17


title: VZA01801D_B05


title: VZA01801D_B03


title: VZA01801D_B10


title: VZA01801D_B01


title: VZA01801D_B04


title: VZA01801D_A22


title: VZA01801D_A23


title: VZA01801D_A21


title: VZA01801D_A24


title: VZA01801D_A20


title: VZA01801D_A19


title: VZA01801D_A14


title: VZA01801D_A16


title: VZA01801D_A13


title: VZA01801D_A15


title: VZA01801D_A18


title: VZA01801D_A12


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title: VZA01801D_A17


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title: VZA01801D_A11


title: VZA01801D_A08


title: VZA01801D_A05


title: VZA01801D_A07


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title: VZA01801D_A02


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title: VZA01713D_L06


title: VZA01713D_M05


title: VZA01713D_L07


title: VZA01713D_L05


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title: VZA01713D_L03


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title: VZA01712D_J23


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title: VZA01712D_J18


title: VZA01712D_J12


title: VZA01712D_K06


title: VZA01712D_J10


title: VZA01712D_J11


title: VZA01712D_J09


title: VZA01712D_M04


title: VZA01712D_J13


title: VZA01712D_J06


title: VZA01712D_J07


title: VZA01712D_J08


title: VZA01712D_J04


title: VZA01712D_J21


title: VZA01712D_J05


title: VZA01712D_J16


title: VZA01712D_J02


title: VZA01712D_I24


title: VZA01712D_J03


title: VZA01712D_I23


title: VZA01712D_I22


title: VZA01712D_J19


title: VZA01712D_I21


title: VZA01712D_I19


title: VZA01712D_J14


title: VZA01712D_I16


title: VZA01712D_I18


title: VZA01712D_I15


title: VZA01712D_I20


title: VZA01712D_I17


title: VZA01712D_I12


title: VZA01712D_I13


title: VZA01712D_I09


title: VZA01712D_I10


title: VZA01712D_I08


title: VZA01712D_I07


title: VZA01712D_J01


title: VZA01712D_I05


title: VZA01712D_I02


title: VZA01712D_I03


title: VZA01712D_I01


title: VZA01712D_H21


title: VZA01712D_H17


title: VZA01712D_H22


title: VZA01712D_I14


title: VZA01712D_H16


title: VZA01712D_H15


title: VZA01712D_H18


title: VZA01712D_I06


title: VZA01712D_H14


title: VZA01712D_H13


title: VZA01712D_H11


title: VZA01712D_I11


title: VZA01712D_I04


title: VZA01712D_H09


title: VZA01712D_H10


title: VZA01712D_H08


title: VZA01712D_H12


title: VZA01712D_H07


title: VZA01712D_H24


title: VZA01712D_H05


title: VZA01712D_H06


title: VZA01712D_H04


title: VZA01712D_H23


title: VZA01712D_H03


title: VZA01712D_H02


title: VZA01712D_H01


title: VZA01712D_H20


title: VZA01712D_H19


title: VZA01712D_G22


title: VZA01712D_G21


title: VZA01712D_G20


title: VZA01712D_G24


title: VZA01712D_G23


title: VZA01712D_G16


title: VZA01712D_G19


title: VZA01712D_G14


title: VZA01712D_G17


title: VZA01712D_G18


title: VZA01712D_G13


title: VZA01712D_G12


title: VZA01712D_G10


title: VZA01712D_G09


title: VZA01712D_G15


title: VZA01712D_G11


title: VZA01712D_G08


title: VZA01712D_G05


title: VZA01712D_G04


title: VZA01712D_G06


title: VZA01712D_G03


title: VZA01712D_G07


title: VZA01712D_G02


title: VZA01712D_G01


title: VZA01712D_F24


title: VZA01712D_F23


title: VZA01712D_F19


title: VZA01712D_F20


title: VZA01712D_F18


title: VZA01712D_F22


title: VZA01712D_F21


title: VZA01712D_F16


title: VZA01712D_F14


title: VZA01712D_F15


title: VZA01712D_F12


title: VZA01712D_F11


title: VZA01712D_F13


title: VZA01712D_F09


title: VZA01712D_F10


title: VZA01712D_F07


title: VZA01712D_F08


title: VZA01712D_F06


title: VZA01712D_F05


title: VZA01712D_F04


title: VZA01712D_F02


title: VZA01712D_E23


title: VZA01712D_F01


title: VZA01712D_F03


title: VZA01712D_E21


title: VZA01712D_E24


title: VZA01712D_E20


title: VZA01712D_E22


title: VZA01712D_F17


title: VZA01712D_E19


title: VZA01712D_E17


title: VZA01712D_E18


title: VZA01712D_E15


title: VZA01712D_E12


title: VZA01712D_E16


title: VZA01712D_E14


title: VZA01712D_E11


title: VZA01712D_E13


title: VZA01712D_E10


title: VZA01712D_E09


title: VZA01712D_E08


title: VZA01712D_E06


title: VZA01712D_E07


title: VZA01712D_E05


title: VZA01712D_E04


title: VZA01712D_E03


title: VZA01712D_E02


title: VZA01712D_D24


title: VZA01712D_E01


title: VZA01712D_D21


title: VZA01712D_D20


title: VZA01712D_D18


title: VZA01712D_D23


title: VZA01712D_D19


title: VZA01712D_D17


title: VZA01712D_D16


title: VZA01712D_D14


title: VZA01712D_D11


title: VZA01712D_D15


title: VZA01712D_D12


title: VZA01712D_D10


title: VZA01712D_D09


title: VZA01712D_D06


title: VZA01712D_D01


title: VZA01712D_C24


title: VZA01712D_C22


title: VZA01712D_D05


title: VZA01712D_D08


title: VZA01712D_C23


title: VZA01712D_C21


title: VZA01712D_D03


title: VZA01712D_D02


title: VZA01712D_C20


title: VZA01712D_C14


title: VZA01712D_C17


title: VZA01712D_C15


title: VZA01712D_C13


title: VZA01712D_C16


title: VZA01712D_C18


title: VZA01712D_C19


title: VZA01712D_D13


title: VZA01712D_D22


title: VZA01712D_C12


title: VZA01712D_D07


title: VZA01712D_C10


title: VZA01712D_C08


title: VZA01712D_D04


title: VZA01712D_C09


title: VZA01712D_C07


title: VZA01712D_C06


title: VZA01712D_C05


title: VZA01712D_C03


title: VZA01712D_C01


title: VZA01712D_B21


title: VZA01712D_B24


title: VZA01712D_B23


title: VZA01712D_B19


title: VZA01712D_B20


title: VZA01712D_B22


title: VZA01712D_B18


title: VZA01712D_B16


title: VZA01712D_C11


title: VZA01712D_B15


title: VZA01712D_B11


title: VZA01712D_B14


title: VZA01712D_B17


title: VZA01712D_B09


title: VZA01712D_B08


title: VZA01712D_B04


title: VZA01712D_C04


title: VZA01712D_B02


title: VZA01712D_B01


title: VZA01712D_B12


title: VZA01712D_B10


title: VZA01712D_A22


title: VZA01712D_B06


title: VZA01712D_B05


title: VZA01712D_B07


title: VZA01712D_B03


title: VZA01712D_B13


title: VZA01712D_A18


title: VZA01712D_A17


title: VZA01712D_A16


title: VZA01712D_A15


title: VZA01712D_A14


title: VZA01712D_A13


title: VZA01712D_A11


title: VZA01712D_A24


title: VZA01712D_C02


title: VZA01712D_A23


title: VZA01712D_A20


title: VZA01712D_A08


title: VZA01712D_A10


title: VZA01712D_A06


title: VZA01712D_A12


title: VZA01712D_A04


title: VZA01712D_A19


title: VZA01712D_A07


title: VZA01712D_A02


title: VZA01712D_A01


title: VZA01707D_P20

value: ETO 30uM


title: VZA01707D_P19


title: VZA01712D_A05


title: VZA01707D_P22

value: ETO 100uM


title: VZA01712D_A03


title: VZA01712D_A21


title: VZA01707D_P18

value: ETO 3uM


title: VZA01707D_P21


title: VZA01707D_P09

value: ETO 10uM


title: VZA01707D_P08


title: VZA01707D_P17


title: VZA01707D_P04


title: VZA01707D_O21


title: VZA01712D_A09


title: VZA01707D_P16

value: ETO 1uM


title: VZA01707D_P15


title: VZA01707D_P13


title: VZA01707D_O22


title: VZA01707D_O19


title: VZA01707D_P12


title: VZA01707D_O16


title: VZA01707D_P11


title: VZA01707D_O18


title: VZA01707D_O14


title: VZA01707D_O12


title: VZA01707D_O11


title: VZA01707D_P07


title: VZA01707D_P06


title: VZA01707D_P03


title: VZA01707D_P05


title: VZA01707D_P10


title: VZA01707D_P02


title: VZA01707D_P01


title: VZA01707D_O07


title: VZA01707D_O05


title: VZA01707D_O02


title: VZA01707D_O04


title: VZA01707D_O03


title: VZA01707D_N22


title: VZA01707D_O20


title: VZA01707D_N19


title: VZA01707D_P14


title: VZA01707D_N20


title: VZA01707D_N17


title: VZA01707D_O15


title: VZA01707D_O17


title: VZA01707D_O13


title: VZA01707D_O10


title: VZA01707D_N16


title: VZA01707D_N14


title: VZA01707D_N13


title: VZA01707D_O09


title: VZA01707D_N08


title: VZA01707D_O06


title: VZA01707D_O01


title: VZA01707D_O08


title: VZA01707D_N18


title: VZA01707D_N04


title: VZA01707D_M21


title: VZA01707D_M20


title: VZA01707D_M15


title: VZA01707D_N11


title: VZA01707D_N12


title: VZA01707D_N09


title: VZA01707D_M11


title: VZA01707D_N10


title: VZA01707D_M14


title: VZA01707D_N15


title: VZA01707D_N06


title: VZA01707D_N05


title: VZA01707D_N07


title: VZA01707D_M10


title: VZA01707D_M08


title: VZA01707D_N21


title: VZA01707D_M04


title: VZA01707D_N02


title: VZA01707D_N01


title: VZA01707D_M19


title: VZA01707D_M18


title: VZA01707D_M22


title: VZA01707D_M17


title: VZA01707D_M13


title: VZA01707D_M16


title: VZA01707D_L22


title: VZA01707D_L21


title: VZA01707D_L20


title: VZA01707D_N03


title: VZA01707D_M06


title: VZA01707D_M07


title: VZA01707D_M09


title: VZA01707D_M05


title: VZA01707D_L15


title: VZA01707D_L14


title: VZA01707D_M12


title: VZA01707D_M03


title: VZA01707D_M01


title: VZA01707D_L17


title: VZA01707D_L18


title: VZA01707D_L16


title: VZA01707D_L07


title: VZA01707D_L05


title: VZA01707D_L04


title: VZA01707D_L12


title: VZA01707D_L02


title: VZA01707D_L10


title: VZA01707D_L09


title: VZA01707D_K20


title: VZA01707D_L13


title: VZA01707D_K19


title: VZA01707D_L11


title: VZA01707D_M02


title: VZA01707D_L19


title: VZA01707D_K08


title: VZA01707D_K12


title: VZA01707D_K06


title: VZA01707D_K13


title: VZA01707D_L03


title: VZA01707D_K22


title: VZA01707D_L01


title: VZA01707D_K21


title: VZA01707D_K15


title: VZA01707D_K18


title: VZA01707D_L08


title: VZA01707D_J19


title: VZA01707D_K16


title: VZA01707D_K14


title: VZA01707D_L06


title: VZA01707D_K11


title: VZA01707D_K04


title: VZA01707D_K05


title: VZA01707D_K10


title: VZA01707D_K09


title: VZA01707D_J13


title: VZA01707D_J11


title: VZA01707D_K07


title: VZA01707D_J22


title: VZA01707D_K01


title: VZA01707D_K02


title: VZA01707D_J09


title: VZA01707D_J21


title: VZA01707D_K03


title: VZA01707D_J06


title: VZA01707D_J05


title: VZA01707D_J08


title: VZA01707D_J04


title: VZA01707D_J20


title: VZA01707D_K17


title: VZA01707D_J18


title: VZA01707D_I18


title: VZA01707D_I10


title: VZA01707D_I09


title: VZA01707D_I05


title: VZA01707D_I03


title: VZA01707D_J17


title: VZA01707D_I02


title: VZA01707D_J16


title: VZA01707D_J15


title: VZA01707D_H22


title: VZA01707D_J12


title: VZA01707D_H19


title: VZA01707D_J10


title: VZA01707D_J14


title: VZA01707D_J07


title: VZA01707D_H18


title: VZA01707D_J03


title: VZA01707D_J01


title: VZA01707D_I21


title: VZA01707D_I20


title: VZA01707D_J02


title: VZA01707D_H14


title: VZA01707D_I19


title: VZA01707D_I17


title: VZA01707D_H11


title: VZA01707D_I16


title: VZA01707D_H13


title: VZA01707D_H10


title: VZA01707D_I15


title: VZA01707D_I14


title: VZA01707D_I13


title: VZA01707D_I22


title: VZA01707D_H07


title: VZA01707D_I11


title: VZA01707D_I08


title: VZA01707D_H05


title: VZA01707D_I07


title: VZA01707D_H01


title: VZA01707D_I06


title: VZA01707D_G19


title: VZA01707D_I04


title: VZA01707D_I01


title: VZA01707D_G18


title: VZA01707D_G16


title: VZA01707D_H21


title: VZA01707D_H20


title: VZA01707D_H17


title: VZA01707D_H16


title: VZA01707D_H15


title: VZA01707D_G10


title: VZA01707D_H09


title: VZA01707D_H12


title: VZA01707D_G05


title: VZA01707D_G03


title: VZA01707D_H06


title: VZA01707D_G01


title: VZA01707D_H08


title: VZA01707D_H02


title: VZA01707D_H03


title: VZA01707D_F21


title: VZA01707D_F22


title: VZA01707D_H04


title: VZA01707D_F19


title: VZA01707D_G22


title: VZA01707D_G21


title: VZA01707D_G20


title: VZA01707D_G13


title: VZA01707D_F17


title: VZA01707D_G17


title: VZA01707D_G15


title: VZA01707D_G12


title: VZA01707D_G09


title: VZA01707D_G14


title: VZA01707D_F14


title: VZA01707D_G08


title: VZA01707D_F11


title: VZA01707D_F12


title: VZA01707D_F10


title: VZA01707D_G07


title: VZA01707D_G06


title: VZA01707D_F06


title: VZA01707D_F02


title: VZA01707D_F08


title: VZA01707D_G04


title: VZA01707D_G02


title: VZA01707D_G11


title: VZA01707D_F20


title: VZA01707D_E22


title: VZA01707D_F16


title: VZA01707D_F18


title: VZA01707D_F15


title: VZA01707D_E16


title: VZA01707D_E14


title: VZA01707D_F13


title: VZA01707D_F07


title: VZA01707D_F05


title: VZA01707D_F04


title: VZA01707D_E08


title: VZA01707D_F03


title: VZA01707D_E07


title: VZA01707D_E04


title: VZA01707D_E05


title: VZA01707D_F01


title: VZA01707D_F09


title: VZA01707D_E02


title: VZA01707D_E21


title: VZA01707D_D21


title: VZA01707D_D20


title: VZA01707D_E20


title: VZA01707D_E19


title: VZA01707D_D19


title: VZA01707D_E17


title: VZA01707D_E18


title: VZA01707D_D18


title: VZA01707D_E15


title: VZA01707D_D16


title: VZA01707D_D14


title: VZA01707D_E03


title: VZA01707D_E13


title: VZA01707D_E12


title: VZA01707D_D08


title: VZA01707D_D12


title: VZA01707D_E11


title: VZA01707D_D05


title: VZA01707D_E10


title: VZA01707D_E09


title: VZA01707D_D02


title: VZA01707D_I12


title: VZA01707D_D17


title: VZA01707D_E06


title: VZA01707D_C20


title: VZA01707D_E01


title: VZA01707D_C18


title: VZA01707D_D22


title: VZA01707D_C17


title: VZA01707D_C16


title: VZA01707D_D15


title: VZA01707D_C13


title: VZA01707D_D11


title: VZA01707D_C11


title: VZA01707D_C12


title: VZA01707D_D13


title: VZA01707D_C22


title: VZA01707D_D07


title: VZA01707D_D04


title: VZA01707D_D06


title: VZA01707D_C04


title: VZA01707D_D03


title: VZA01707D_C05


title: VZA01707D_C21


title: VZA01707D_B22


title: VZA01707D_D10


title: VZA01707D_C19


title: VZA01707D_D09


title: VZA01707D_C15


title: VZA01707D_C10


title: VZA01707D_B13


title: VZA01707D_B12


title: VZA01707D_C09


title: VZA01707D_B11


title: VZA01707D_D01


title: VZA01707D_C08


title: VZA01707D_B10


title: VZA01707D_C07


title: VZA01707D_C06


title: VZA01707D_C03


title: VZA01707D_C02


title: VZA01707D_C01


title: VZA01707D_B03


title: VZA01707D_B20


title: VZA01707D_B21


title: VZA01707D_C14


title: VZA01707D_B19


title: VZA01707D_B18


title: VZA01707D_B16


title: VZA01707D_B17


title: VZA01707D_B15


title: VZA01707D_A21


title: VZA01707D_A19


title: VZA01707D_A16


title: VZA01707D_A15


title: VZA01707D_A13


title: VZA01707D_B02


title: VZA01707D_B14


title: VZA01707D_A10


title: VZA01707D_B09


title: VZA01707D_B06


title: VZA01707D_B05


title: VZA01707D_B08


title: VZA01707D_B07


title: VZA01707D_A08


title: VZA01707D_B04


title: VZA01707D_A06


title: VZA01707D_A01


title: VZA01707D_A02


title: VZA01707D_B01


title: VZA01707D_A22


title: VZA01707D_A20


title: VZA01707D_A18


title: VZA01707D_A17


title: VZA01706D_P20


title: VZA01706D_P18


title: VZA01707D_A14


title: VZA01706D_P17


title: VZA01707D_A12


title: VZA01706D_P16


title: VZA01706D_P12


title: VZA01707D_A07


title: VZA01707D_A09


title: VZA01707D_A04


title: VZA01707D_A05


title: VZA01706D_P23


title: VZA01706D_P22


title: VZA01706D_P24


title: VZA01707D_A03


title: VZA01706D_P08


title: VZA01707D_A11


title: VZA01706D_P03


title: VZA01706D_P14


title: VZA01706D_O21


title: VZA01706D_P11


title: VZA01706D_P09


title: VZA01706D_P19


title: VZA01706D_P13


title: VZA01706D_P15


title: VZA01706D_O20


title: VZA01706D_P21


title: VZA01706D_P06


title: VZA01706D_P04


title: VZA01706D_P05


title: VZA01706D_P02


title: VZA01706D_O17


title: VZA01706D_O15


title: VZA01706D_O13


title: VZA01706D_O12


title: VZA01706D_O22


title: VZA01706D_P01