title: Dcaf1-deficien Tconv 1 [Wan-029_L5.D712]

city: Chapel Hill

first: Zengli

last: Guo

state: NC

address: 125 Mason Farm Road

department: Lineberger Comprehensive Cancer Center

institute: University of North Carolina at Chapel Hill

data_processing:
Reads (32-45 Million reads per sample) were analyzed with Salmon [version 0.9.1] (Patro et al., 2017) software to align and quantify the transcript expression.
R packages in Bioconductor, tximport and tximportData (Soneson et al., 2015) were used to aggregate transcript-level quantifications to the estimated counts at the gene level, with the R package biomaRt for gene and transcripts mapping. The option "lengthScaledTPM" for countsFromAbundance in tximport was used.
DESeq2 (Love et. al.,  2014) in R was further used to analyze gene differential expression and generate normalized expression data at gene level.
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files includer counts at gene level and normalized expression from DESeq2 analysis

source_name: Dcaf1-deficient_KO Tconv

growth_protocol: Primary Treg and Tconv cells isolated from indicated mice

extract_protocol:
Total RNA of FACS sorted Treg and Tconv cells from indicated mice was extracted by using RNA-easy mini kit (Qiagen).
RNA-seq libraries were generated and poly(A) enriched with 1 microgram of RNA as input using the TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA).

treatment_protocol: CD4+CD25+ Treg cells and CD4+ Tconv cells were sorted by FACS

tag: strain background

value: 2 months

tag: tissue

value: spleen


title: ChIP_WT_FastAndSlow_Neu_NIPBL_rep3_UT

status: Public on Oct 10 2020

type: SRA

city: La Jolla

first: Cornelis

last: Murre

state: CA

address: 9500 Gilman Drive

department: Molecular Biology

country: USA

institute: UCSD

description: Immunoprecipitated DNA

library_source: genomic

data_processing:
HiC analysis. HiC reads were processed using HiC-Pro analysis pipeline (Servant et al., 2015). In brief, reads were aligned to mm9 with Bowtie2 with 'very sensitive' mode and reads with mapping quality (MAPQ) > 1 were kept for downstream analysis. Hi-C statistics was summarized (Wingett et al. 2015) (Supplemental Table S1). After stringent data filtering, including removal of PCR duplicates, self-ligation, restriction ends, continuous genomic fragments and re-ligation events, valid contacts file was converted to HIC files using the hicpro2juicebox.sh script. The balanced and normalized contact matrix was visualized by Juicebox (Durand et al., 2016b). PCA analysis by HOMER, applied to the normalized interaction matrix, and PC1 values for each 25-kb bin with positive and negative values, corresponding to A and B compartments, respectively. Genomic regions that displayed a continuum of either positive or negative PC1 values were defined as PC1 domains (PDs). PDs with minimum size of 100kb were identified by findHiCCompartments.pl using default settings. We consider the absolute difference in PC1 value (> 0.4) and the PC1 value change from positive to negative or vice versa to identify PDs that switch compartments.
ChIP-Seq and ATAC-Seq analysis. Reads were aligned to mm9 with Bowtie2 and reads non-uniquely mapped were discarded. Data were analyzed with HOMER. Tag directory was made by makeTagDirectory under the default setting, using option -tbp 1 -fragLength 150. Genome browser visualizing files were made by makeUCSCfile under the default setting, using option -fragLegth 150, -strand both. Bigwig files was created from bedgraph using UCSC utility bedGraphToBigWig. Meta-gene profile was made by makeMetaGeneProfile.pl under the default setting, using option -fraglength 150, -pc 1, -gbin 100 (small genes less than 3kb were excluded).  Average enrichment plot was made by annotatePeaks.pl under the default setting, using option -fraglength 150, -pc 1, -hist 100. Heatmaps were created by Deeptools package in 'reference-Point' mode, using option -bin size 100 -missingDataAsZero -skipZeros. ChIP-Seq peaks were identified by findPeaks in 'factor' mode under the default setting against the input directory, using option -fraglength 150, -size 200. Peaks from separate experiments were considered co-bound if their peak centers were located within 100_bp of each other. Peaks were merged by mergePeaks.pl. Differential peaks were identified by getDifferentialPeaks under the default setting, using option -size 200. Genomic annotations of peaks were computed with annotatePeaks.pl under the default setting. To identify motifs enriched in peak regions over the background, HOMER's motif analysis (findMotifsGenome.pl) including known default motifs and de novo motifs was used with option -mis 0 -len 10,12 -size 200 -mask -p 12 -cache 50000. The background sequences used for motif analysis either from random genome sequences of the same size and normalized GC content or from peaks in comparing condition were indicated in the figure legends. Scatterplots were used for direct comparison of two conditions, either the gene RPKM or normalized tag counts were used for ChIP-/ATAC-Seq respectively.
GRO-Seq analysis. Reads were trimmed 20-bp off the 3' end of the sequence to improve mappability. Reads were aligned to mm9 with Bowtie2 (Langmead and Salzberg, 2012) and reads non-uniquely mapped were discarded. Data were analyzed with HOMER (Heinz et al., 2010). Tag directory was made by makeTagDirectory under the default setting, using option -tbp 3 -fragLength 150, in order to effectively remove the super spikes but still allow genes with high RPKM to "express themselves" in the following analysis. Sequencing-depth normalized bedGraph files for visualization in the UCSC Genome Browser were made by makeUCSCfile under the default setting, using option -norm 1e7, -fragLegth 150, -strand + or -strand -, respectively. Strand-specific bigwig files was created from bedgraph using utility bedGraphToBigWig. The RefFlat gene expression values were quantified by analyzeRepeats under the default setting, using option -condenseGenes -count genes -rpkm -strand separate. Genes with RPKM value less than 0.5 in all conditions were defined as not expressed (8733 genes were expressed). Functional enrichment calculations were performed using PANTHER web portal (http://www.pantherdb.org) applying 'Overrepresentation Test' using the Fisher's exact test and FDR correction method. Meta-gene profile was made by makeMetaGeneProfile.pl under the default setting, using option -fraglength 150, -pc 3, -gbin 100. Average distribution histogram was made by annotatePeaks.pl under the default setting, using option -fraglength 150, -pc 3, -hist 100. Heatmaps were created by Deeptools package in scale-region mode, -bin size 100.
Genome_build: mm9
Supplementary_files_format_and_content: Processed files include BED files (ChIP-Seq peak file, Hi-C PC1 domain positions), RPKM CSV files (GRO-Seq expression data), Bedgraph and Bigwig (ChIP-Seq, GRO-Seq and ATAC-Seq tracks, principle component values along the genome for combined Hi-C data), and tab-delimited txt files, including HIC files (filtered read pairs from Hi-C data processed using HiC-Pro)

molecule: genomic DNA

organism: Mus musculus

source_name: in-vitro differentiated neutrophils

growth_protocol: GM-CSF-dependent ECOMG cells were grown in RPMI 1640 with 10% FBS and penicillin-streptomycin-glutamine, by the addition of 1:100 conditioned media (about 10 ng/mL GM-CSF) isolated from a B16 melanoma cell line stably transfected with a murine GM-CSF construct. b-estradiol where applicable, was added to the medium at a final concentration of 1 mM from a 10,000 × stock in 100% ethanol. Differentiation of ECOMG cells into granulocytes was induced by withdrawal β-estradiol for 5 days. Adult C57BL/6 mice were purchased from Jackson Laboratories and maintained in a specific pathogen-free facility at the University of California, San Diego. Mice were used between 8-12 weeks of age. Both male and female mice were used for study. Primary neutrophils were isolated from bone marrow as described (Zhu et. al. 2017). Primary B and T cells from spleens were isolated using negative-selection kits, Miltenyi Biotec 130-090-862 and 130-095-130 on MACS LS columns, respectively.

extract_protocol:
The ChIP-Seq experiments were performed similarly as described (Lin YC, et al. 2012. Global changes in the nuclear positioning of genes and intra- and interdomain genomic interactions that orchestrate B cell fate. Nat Immunol 13: 1196-1204).
ChIP-Seq libraries were prepared with the NEBNext primer set and were selected by size by 8% PAGE and sequenced for 75 or 100 cycles on Illumina HiSeq 4000.

treatment_protocol: In vitro differentiated neutrophils from ECOMG cells were used as a model system to study nuclear architecture in response to calcium influx in neutrophils. ECOMG cells were cultured and differentiated as described (Zhu et. al. 2017). For inhibitors, neutrophils were treated with 10uM TRP or 100uM DRB for 30min, or 2uM FK506 for 2h before activation. Primary B cells were treated with 2uM FK506 for 2h before activation. Inhibitors were kept in culture during activation time course. Acute degradation was induced by treating the cells with a final concentration of 500 nM dTAG-13 molecule Tocris). dTAG-13 was kept in culture during activation time course. All the time series experiments were performed by inducing protein degradation at the beginning of the time course and harvest the samples at the different time points. RPMI-1640 contains 0.42mM calcium. Additional CaCl2 was supplied to the medium during activation to a final concentration of 1mM. Neutrophils were activated either in fast activation condition (20uM A23187 for 15min), or in slow activation condition (5uM A23187 for 4h). These two activation conditions were used throughout the manuscript unless otherwise mentioned. The time series activation experiments were performed by activation neutrophils at the beginning of the time course and harvest the samples at the different time points.

tag: strain

tag: genotype

value: human E2A_ER_Pbx1b, wild type

tag: cell type

value: ECOMG derived neutrophils

tag: treatment

value: untreated

tag: chip antibody

value: NIPBL A301-779A  Bethyl Laboratries


title: SF3B1WT_n2_HEK

city: Paris

first: Alexandre

last: Houy

address: 26 rue d'Ulm

country: France

institute: Institut Curie

library_source: transcriptomic

data_processing:
fastq files were mapped on Human genome (hg19) using STAR (v2.5.3a) with default parameters and with –quandMode GeneCounts
Genome_build: hg19
Supplementary_files_format_and_content: tab delimited text files containing the number of reads per gene (_ReadsPerGene.out.tab). _ReadsPerGene.out.tab format: column 1: gene ID;column 2: counts for unstranded RNA-seq;column 3: counts for the 1st read strand aligned with RNA (htseq-count option -s yes);column 4: counts for the 2nd read strand aligned with RNA (htseq-count option -s reverse).
Supplementary_files_format_and_content: tab delimited text files containing the number of reads per splice junction (_SJ.out.tab).  _SJ.out.tab format: column 1: chromosome column 2: first base of the intron (1-based) column 3: last base of the intron (1-based) column 4: strand (0: undefined, 1: +, 2: -) column 5: intron motif: 0: non-canonical; 1: GT/AG, 2: CT/AC, 3: GC/AG, 4: CT/GC, 5: AT/AC, 6: GT/AT column 6: 0: unannotated, 1: annotated (only if splice junctions database is used) column 7: number of uniquely mapping reads crossing the junction column 8: number of multi-mapping reads crossing the junction column 9: maximum spliced alignment overhang.

molecule: total RNA

organism: Homo sapiens

source_name: HEK293T cells transfected with Wildtype SF3B1 expression vector (replicate n=2)

growth_protocol: HEK293T and HAP1 cell lines were cultured in DMEM supplemented with 10% fetal bovine serum.

extract_protocol:
Total RNA was extracted with NucleoSpin RNA kit (Macherey-Nagel). The quantity and quality of RNA was determined by spectrophotometry (NanoDrop Technologies). Five hundred nanograms of RNA was used as a template for cDNA synthesis with the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems).
Libraries were constructed using the TruSeq Stranded mRNA Sample Preparation Kit (Illumina) and sequenced on an Illumina HiSeq 2500 platform using a 100-bp paired-end sequencing strategy.

treatment_protocol: A point mutation in SUGP1 resulting in P636L amino-acid substitution was introduced using CRISPR/cas9-stimulated homology-directed repair to generate isogenic HAP1 cell lines. A second point mutation in ATP1A1 was simultaneously introduced conferring ouabain resistance. Cells were transfected with preformed Cas9 ribonucleoprotein complexes (RNPs) containing synthetic crRNAs specific for ATP1A1 and SUGP1 along with ssODNs-specified mutation at a 3:1 ratio (Integrated DNA Technologies, Inc, IDT). Sequence of gRNA: TGGTACGCATGTCCTCCTGG; donor sequence: TCCTACCACCACCCTCTCCTGTCGACTGAGCAAAGGCAGCCCAGGAGGACATGCGTACCAGGAGGTTGGGCCGGAAGCGGTAGGCCAGCATCATCCTCTTGCGGAACGCCTCATACTCGTCGTCCTCC. Cells were selected and expanded in the presence of ouabain (0.5µM) and verified by Sanger sequencing.  Plasmid transfections were carried out in cell lines using 500 ng of plasmid construct and Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer’s instructions. For siRNA-mediated knockdown, cells were transfected with 50nM of siRNA using lipofectamine RNAiMAX (Invitrogen) and the following siRNA: siSUGP1 (Cat.No. s33721, Ambion; and GeneSolution Cat.No. 1027416, Qiagen), or control siRNA (Cat.No. S103650318).

tag: cell line

tag: transfection


title: ChIP_WT_FastAndSlow_Neu_NIPBL_rep2_A23slow4h

description: Immunoprecipitated DNA

value: slow_treated with 5uM A23187 for 4h


title: Aged Tconv 2 [Wan-028_L3.D711]

source_name: wild type_Old Tconv

tag: age

value: > 18 months

tag: genotype/variation

value: wild type


title: ChIP_WT_FastAndSlow_Neu_NIPBL_rep3_A23fast15min

description: Immunoprecipitated DNA

value: fast_treated with 20uM A23187 for 15min


title: ChIP_WT_FastAndSlow_Neu_NIPBL_rep1_A23slow4h

description: Immunoprecipitated DNA


title: CEMBA190305_11F_1_CEMBA190305_11F_2_A10_ad002

first: Joseph

middle: R

last: Ecker

address: 10010 North Torrey Pines Road

department: Genomic Analysis Laboratory

institute: HHMI-Salk-Institute

data_processing:
Library strategy: snmC-seq2
Reads trimed with Cutadapt for adapters and low quality bases (Phred score >= 20)
The resulting files are mapped to in-silico bisulfite converted mm10 reference genome using Bismark.
The raw BAM file will be filtered using samtools to filter reads by MAPQ > 10 and use Picard to remove PCR duplicates
Tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls will be generated with in-house developing package using the filtered BAM files
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls. Columns are: 1. Chromosome, 2. Position, 3. Strand, 4. Cytosin Context, 5. methylated base call, 6. total mapped base call, 7. not used, place holder column

source_name: C57BL/6J Male P56 Mouse Brain

growth_protocol: All mice are C57BL/6J Male, brain dissected in P56

extract_protocol:
the brain of P56 male C57BL/6J mouse will be removed and cut into 600 microns thick coronal sections using a tissue matrix. Then each region will be microdissected from the coronal tissue sections based on identified landmarks and brain atlas coordinates, as defined in Allen brain atlas CCF3 (Avaliable at: http://atlas.brain-map.org). For each dissection region, the tissue from several littermate mice will be combined together into to separate replicate groups. The number of animals in a replicate depends on the size of the tissue. Nuclei preparation will be stained with the fluorescently labeled anti-NeuN body and nucleic acid stain Hoechst 33342.
Library prepared following protocol discribed in Luo, Chongyuan, Angeline Rivkin, Jingtian Zhou, Justin P. Sandoval, Laurie Kurihara, Jacinta Lucero, Rosa Castanon, et al. 2018. “Robust Single-Cell DNA Methylome Profiling with snmC-seq2.” Nature Communications 9 (1): 3824.

tag: brain region

value: DG

tag: Sex

value: male


title: Young Tconv 2 [Wan-026_L5.D710]

source_name: wild type_Young Tconv


title: ChIP_WT_FastAndSlow_Neu_NIPBL_rep1_A23fast15min

description: Immunoprecipitated DNA


title: Dcaf1-deficien Tconv 2 [Wan-030_L5.D701]


title: ChIP_WT_FastAndSlow_Neu_NIPBL_rep2_UT

description: Immunoprecipitated DNA


title: Aged Tconv 1 [Wan-027_L5.D711]


title: CEMBA190305_11F_1_CEMBA190305_11F_2_A10_ad007


title: CEMBA190305_11F_1_CEMBA190305_11F_2_A10_ad006


title: CEMBA190305_11F_1_CEMBA190305_11F_2_A1_ad012


title: siCTRL_n1_HEK

source_name: HEK293T cells transfected with siRNA Control (replicate n=1)

value: transfected with siRNA Control


title: siSUGP1_n1_HEK

source_name: HEK293T cells transfected with siSUGP1 (replicate n=1)

value: transfected with siSUGP1


title: ChIP_WT_FastAndSlow_Neu_NIPBL_rep2_A23fast15min

description: Immunoprecipitated DNA


title: SF3B1mut_n1_HEK

source_name: HEK293T cells transfected with SF3B1-K700E expression vector (replicate n=1)


title: CEMBA190305_11F_1_CEMBA190305_11F_2_A10_ad004


title: Young Tconv 1 [Wan-025_L4.D709]


title: CEMBA190305_11F_1_CEMBA190305_11F_2_A1_ad008


title: SF3B1WT_n1_HEK

source_name: HEK293T cells transfected with Wildtype SF3B1 expression vector (replicate n=1)


title: HAP1mutSUGP1_n3

source_name: Mutant SUGP1 CRISPR HAP1 cells (replicate n=3)

value: Mutant SUGP1 CRISPR


title: ChIP_WT_FastAndSlow_Neu_NIPBL_rep1_UT

description: Immunoprecipitated DNA


title: Dcaf1-deficient Treg 1 [Wan-023_L4.D707]

source_name: Dcaf1-deficient_KO Treg


title: SARS-CoV-2 infected Vero Cells at day 2, replicate 2

city: Daejeon

first: Young Seok

last: Ju

address: Daehak-ro 293

country: South Korea

institute: KAIST

data_processing:
STAR ver. 2.6.1
RSEM ver. 1.3.1
Genome_build: Chlab1.1, NC_045512.2, yeast ENO2 cDNA (SGD:YHR174W), U13369.1
Supplementary_files_format_and_content: Matrix table with each colum indicating gene id, transcript id, gene length, gene effective length, raw count, TPM, and FPKM.

organism: Chlorocebus aethiops

source_name: kidney

extract_protocol:
RNeasy Plus Mini Kit
Truseq Stranded Total RNA Gold kit

value: Cell line

value: 48 hours after SARS-CoV-2 infection


title: HAP1_n3

source_name: Wild Type HAP1 cells (replicate n=3)

value: Wild Type


title: HAP1mutSUGP1_n2

source_name: Mutant SUGP1 CRISPR HAP1 cells (replicate n=2)


title: HiC_Neu_A23fast30min_rep1

description: genomic interactions

extract_protocol:
In situ Hi-C was performed using the MboI restriction enzyme as previously described without modifications  (Rao SSP, et al. 2014. A 3D map of the human genome at kilobase resolution reveals principles of chromatin looping. Cell 159: 1665-1680).
Hi-C libraries were prepared with the NEBNext primer set and were selected by size by 6% PAGE and sequenced for 100 cycles on Illumina HiSeq 4000 or 50 cycles on Illumina NovaSeq.

value: fast_treated with 20uM A23187 for 30min


title: SARS-CoV-2 infected Vero Cells at day 2, replicate 1


title: CEMBA190305_11F_1_CEMBA190305_11F_2_A1_ad010


title: CEMBA190305_11F_1_CEMBA190305_11F_2_A1_ad007


title: Dcaf1-deficien Treg 2 [Wan-024_L4.D708]

value: 2 months

value: spleen


title: Aged Treg 2 [Wan-022_L3.D710]

source_name: wild type_Old Treg


title: CEMBA190305_11F_1_CEMBA190305_11F_2_A1_ad006


title: SARS-CoV-2 infected Vero Cells at day 1, replicate 2

value: 24 hours after SARS-CoV-2 infection


title: Aged Treg 1 [Wan-021_L4.D706]


title: HAP1_n2

source_name: Wild Type HAP1 cells (replicate n=2)


title: SARS-CoV-2 infected Vero Cells at day 0 (control), replicate 1

value: 0 hours after SARS-CoV-2 infection


title: CEMBA190305_11F_1_CEMBA190305_11F_2_A1_ad001


title: Young Treg 1 [Wan-019_L4.D704]

source_name: wild type_Young Treg


title: Young Treg 2 [Wan-020_L4.D705]


title: C_7

status: Public on Oct 09 2020

city: Princeton

first: Ian

middle: Michael

last: Traniello

state: NJ

address: Princeton University

department: Lewis-Sigler Insitute for Genomic Biology

institute: Princeton University

data_processing:
All samples were processed using "CRISPResso2" (Clement et al. 2019) in "batch mode" using the following command:
/home/traniel2/anaconda3/envs/crispr/bin/CRISPRessoBatch --batch_settings batch.txt --amplicon_seq GGTCCACCTGGTCCTGATACTGatgcagttcggattctgcggtatcaatctaatgatggagagcgaagacgtggacgatctcaccgcgaacaccatcaccatgctcttcttcacgcacagcgtggtcaagctcgtttacttcgcggtcaggagtaaattgttctacagaacgcttggcatatggaacaatccgaacagccatcccctcttcgccgagagcaacgcgcgataccatcagatagccgtcaagaagatgaggatactcctgctggccgtcatagggaccacggtgctgtccgccatttcttggaccaccatcactttcattggtgactctgtgaaaaaggtcatcgaccctgtcaccaacgaaacGACCTACGTCGAGGTAATCCAA --guide_seq tgctcttcttcacgcacagc -p 4 --exclude_bp_from_right 22 --exclude_bp_from_left 22 --quantification_window_center -17 --min_frequency_alleles_around_cut_to_plot .5
>orco amplicon GGTCCACCTGGTCCTGATACTGatgcagttcggattctgcggtatcaatctaatgatggagagcgaagacgtggacgatctcaccgcgaacaccatcaccatgc-tcttcttcacgcacagcgtggtcaagctcgtttacttcgcggtcaggagtaaattgttctacagaacgcttggcatatggaacaatccgaacagccatcccctcttcgccgagagcaacgcgcgataccatcagatagccgtcaagaagatgaggatactcctgctggccgtcatagggaccacggtgctgtccgccatttcttggaccaccatcactttcattggtgactctgtgaaaaaggtcatcgaccctgtcaccaacgaaacGACCTACGTCGAGGTAATCCAA
dash is Cas9 cut site, capital letters are primers
guide seq: tgctcttcttcacgcacagc
Genome_build: HAv3.1
Supplementary_files_format_and_content: Pickle file contains info from all samples run in "batch" mode

organism: Apis mellifera

source_name: antenna

growth_protocol: Honey bee embryos were injected with either a CRISPR RNP complex targeting the orco gene or injection buffer (control). Bees were reared in the lab and dissected within 24 hours after eclosion.

extract_protocol:
For molecular genotyping, we have used a quick method of DNA extraction from the adults antennal using NucleoType Mouse PCR kit (Cat No. 743200)
For deep sequencing, the library was constructed using the protocol outlined in the Illumina 16S metagenomic library preparation. The first stage PCR was performed with the orco specific primers with overhang adapters at the 5’ end and second-stage PCR was performed with Nextera unique dual (UD) indexing kit. Primer sequences used for the first round of PCR were:    Apis_Orco_16770F2       5’TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGggtccacctggtcctgatactg    Apis_Orco_17163R2       5’GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGttggattacctcgacgtaggtc
* The library pool was quantitated by qPCR and sequenced on one MiSeq Micro flowcell for 251 cycles from each end of the fragments using a MiSeq 500-cycle sequencing kit version 2.
Fastq files were generated and demultiplexed with the bcl2fastq v2.20 Conversion Software (Illumina)
Phix DNA is used as a spike-in control for MiSeq runs. Most Phix reads are removed during processing but a few residual reads may remain. Phix genome is 3kb in length.

value: buffer-injected control

value: antenna

tag: development stage

value: adult


title: HAP1mutSUGP1_n1

source_name: Mutant SUGP1 CRISPR HAP1 cells (replicate n=1)


title: SARS-CoV-2 infected Vero Cells at day 0 (control), replicate 2


title: Chimp1_2_CNCCs

city: Jerusalem

first: David

last: Gokhman

address: Givat Ram, Safra Campus

country: Israel

institute: The Hebrew University of Jerusalem

data_processing:
Remove sequencing adapters with SeqPrep version 1.2
Map reads to GRCh38 and PanTro5 with STAR version 2.5.1b
Remove duplicate reads with Picard 2.18.27
Remove reads which exhibit mapping bias with WASP (Hornet)
Count allele specific and total reads
Genome_build: Human: GRCh38; Chimpanzee: PanTro5
Supplementary_files_format_and_content: Counts matrix with total and allele-specific raw read counts (rows are genes, there are 4 columns per sample: ref counts (aligned to reference genome), alt counts (aligned to alternative genome), no ASE (no segregating SNPs) and ambiguous (SNPs from both genome); matrix text files are provided for samples mapped on both the human and chimpanzee genomes; text files with sample meta data are also included for each data set
Supplementary_files_format_and_content: GRCh38 (1st sheet), panTro5 (2nd sheet)
Supplementary_files_format_and_content: Raw read counts per gene across all samples

molecule: polyA RNA

organism: Pan troglodytes

source_name: iPSC-derived CNCCs

growth_protocol: Three independent CNCC differentiation experiments were performed. In each one, human-chimpanzee hybrid iPSC (Hy1_30), parental human (Hu1), parental chimpanzee (Ch1) iPSC lines, and human embryonic stem cells (hESCs, control) were differentiated into CNCCs. Briefly, iPSCs and hESCs were incubated with 2mg/ml collagenase for ~30-50 min leading to detachment of colonies. Detached cells were plated as clusters of 100-200 cells in low-attachment petri dishes and cultured in the presence of CNCC differentiation medium consisting of 1:1 Neurobasal medium/DMEM F-12 medium (ThermoFisher Scientific), 0.5× B-27 supplement with Vitamin A (50× stock, GeminiBio), 0.5× N-2 supplement (100× stock, GeminiBio), 20 ng/ml bFGF (Peprotech), 20 ng/ml EGF (Sigma-Aldrich), 5 µg/ml bovine insulin (Sigma-Aldrich) and 1× Glutamax-I supplement (100× stock, ThermoFisher Scientific). Cells grown in CNCC differentiation medium grew as neural spheres/rosettes. For the first four days of differentiation, spheres were separated from cell debris by gentle centrifugation and re-plated into new petri dishes in fresh CNCC differentiation medium. After four days, the neural spheres were allowed to settle for three days to promote attachment to the culture plate surface. After the neural spheres began to attach to the plate, media was changed daily, and neural crest cells were allowed to migrate out of the neural rosettes for 4-5 days. Afterwards, neuroectodermal spheres were manually picked and removed from the culture dishes leaving behind emigrated neural crest cells, which were dissociated with 1x Accutase and passaged onto fibronectin (7.5µg/ml) (ThermoFisher Scientific) coated plates. The early migratory CNCCs were cultured in the presence of maintenance medium comprising of 1:1 Neurobasal medium/DMEM F-12 medium (Invitrogen), 0.5× B-27 supplement with Vitamin A (50× stock, GeminiBio), 0.5× N-2 supplement (100× stock, GeminiBio), 20 ng/ml bFGF (Peprotech), 20 ng/ml EGF (Sigma-Aldrich), 1 mg/ml bovine serum albumin, serum replacement grade (Gemini Bio-Products # 700-104P) and 1× Glutamax-I supplement (100× stock, ThermoFisher Scientific). The CNCCs were cultured on fibronectin coated dishes, with passaging every three days with 1x Accutase for additional two passages. Afterwards, medium was changed to BMP/ChIR medium by adding 3µM ChIRON 99021 (Selleck, CHIR-99021) and 50pg/ml BMP2 (Peprotech) to the maintenance medium, which increased cell proliferation and decreased migration.

extract_protocol:
Qiagen Rneasy Mini Kit with on-column DNAse digestion (Qiagen)
Illumina TruSeq Stranded mRNA library prep kit (Illumina)


title: HAP1_n1

source_name: Wild Type HAP1 cells (replicate n=1)


title: C_6


title: Chimp1_3_CNCCs


title: Chimp1_1_CNCCs


title: orco 73

value: Orco-KO


title: CEMBA190305_11F_1_CEMBA190305_11F_2_A1_ad004

source_name: C57BL/6J Male P56 Mouse Brain

value: DG


title: C_2


title: C_1


title: C_5


title: orco 71


title: C_3


title: orco 72


title: C_4


title: SARS-CoV-2 infected Vero Cells at day 1, replicate 1


title: orco 70


title: orco 68


title: orco 64


title: orco 67


title: orco 66


title: orco 62


title: orco 63


title: orco 65


title: orco 57


title: orco 60


title: orco 59


title: orco 58


title: orco 56


title: orco 52


title: orco 53


title: orco 54


title: orco 55


title: orco 51


title: orco 69


title: orco 50


title: orco 75


title: orco 76


title: orco 49


title: orco 48


title: orco 74


title: orco 47


title: orco 45


title: orco 43


title: orco 44


title: orco 61


title: orco 42


title: orco 40


title: orco 46


title: orco 41


title: orco 39


title: orco 38


title: orco 36


title: orco 33


title: orco 32


title: orco 31


title: orco 37


title: orco 34


title: orco 28


title: orco 26


title: orco 29


title: orco 30


title: orco 27


title: orco 24


title: orco 25


title: orco 22


title: orco 23


title: orco 21


title: orco 20


title: orco 19


title: orco 18


title: orco 17


title: orco 15


title: orco 16


title: orco 13


title: orco 14


title: orco 11


title: orco 10


title: orco 9


title: orco 35


title: orco 7


title: orco 8


title: orco 5


title: orco 3


title: orco 4


title: orco 1


title: Berberine+Thymol

city: Istanbul

first: Cemile

middle: Selin

last: Aksoy

address: Marmara Uni, Bioengineering Dep., No:404 Kadıköy

department: Bioengineering

country: Turkey

institute: Marmara University

data_processing:
The raw reads were filtered.Data filtering includes removing adapter sequences, contamination and low-quality reads from raw reads
Sequencing reads were aligned to the chromosomal region in the genome of S. aureus strain ATCC 25923 using the quantify and annotate short reads (QuasR) package in R
Differential expression analyses were performed in R version 3.5.0 R using the package DESeq2
Genome_build: RefSeq accession number NZ_CP009361.1
Supplementary_files_format_and_content: raw counts; log2 values of differentially expressed genes

organism: Staphylococcus aureus

source_name: Staphylococcus aureus

growth_protocol: Cells were grown in mueller hinton broth at 37°C and 180 rpm.

extract_protocol:
One-hour berberine&thymol and DMSO treated cells were placed in RNAprotect Bacteria Reagent (Qiagen, Germany) and incubated for 5 minutes at room temperature. Cell suspensions were centrifuged at 5,000xg for 10 minutes. Total RNA isolation was achieved using RNeasy Mini Kit (Qiagen, Germany) according to manufacturer’s protocol with mechanical disruption at 6 m/s for 120 seconds (FastPrep Instrument, MP Biomedicals, USA). At least two biological replicates were mixed in one sample tube for each condition prior to library preparation.
RNA libraries were prepared for sequencing using standard Illumina protocols

treatment_protocol: Cells were treated with 32 mg/ L of berberine chloride hydrate and 64 mg/L of thymol when OD600 reached 0.3±0.05.

value: Berberine+Thymol


title: Hi-C_Forelimb-R1

city: Berlin

first: Francisca

last: Martinez Real

address: Ihnestrasse 73

country: Germany

institute: Max Planck Institute for Molecular Genetics

data_processing:
Raw reads from sequenced Hi-C libraries were processed using the Juicer pipeline v1.5.6., which was set up with the short read aligner BWA 0.7.17. The two replicates were processed independently and combined afterwards by merging the filtered and deduplicated read-pairs.
Genome_build: talOcc4
Supplementary_files_format_and_content: hic

organism: Talpa occidentalis

source_name: Forelimb

extract_protocol:
Five million cells were used to generate each library of Hi-C as previously described (Rao 2014). Two independent libraries from two different pool of individuals were generated following the in-situ Hi-C protocol from Rao 2014 without modifications.
Whole fixed gonads were sonicated with a Bioruptor (45 cycles 30sec on, 30 sec off at high power) in the shearing buffer from the kit. 5 mg of chromatin was used in each immunoprecipitation with 1mg of the following specific histones antibodies: anti-H3K4me3, Millipore, cat. No. 07-473; anti-H3K4me1, Diagenode, cat. No. C15410037; anti-H3K27ac, Diagenode, cat. No. C15410174; anti-H3K27me3, Millipore, cat. No. 07-449. Libraries were prepared using the Nextera adaptors and sequenced

treatment_protocol: Forelimb buds from mole embryos of around 18 days of gestation were disaggregated with trypsine, filter with a cell strainer to obtain single cell suspension and fixed in 2% formaldehyde.

value: Wild type

tag: developmental stage

value: Embryonic stage 18

value: Forelimb


title: DMSO

value: DMSO


title: Hi-C_Forelimb-R2


title: ATAC-TestisPortion-P7-Wt-To-R1-L7299_S3

data_processing:
Reads of each ATAC-seq sample were mapped using STAR v2.6.1d. In order to force an unspliced end-to-end alignment of the 50bp reads and to report only unique alignments the following options were applied: --alignEndsType EndToEnd   alignIntronMax 1   outFilterMatchNminOverLread 0.94 --outFilterMismatchNmax 5 --outFilterMultimapNmax 1.
The data was segmented into 5 states via EpiCseg (Mammana et al., 2015).  If a state was supported by <50 regions the respective genomic regions were excluded from segmentation and EpiCseg was rerun.
Genome_build: talOcc4, mm9

source_name: Testicular part of the ovotestis

extract_protocol:
50.000 cells were used for each reaction of tagmentation and protocol was performed as previously described (Buenrostro JD et al., 2015).
Libraries were prepared using the Nextera adaptors and sequenced

treatment_protocol: Gonads from P7 mole animals and from E13.5 mouse embryos were disaggregated in PBS using a disruptor and filter through a cell strainer to get single cell suspension.

tag: embryonic stage

value: Post-partum day 7


title: ATAC-TestisPortion-P7-Wt-To-R2-L7300_S4


title: ATAC-Testis-P7-Wt-To-R2-L7280_S2

source_name: Testis


title: ATAC-Testis-P7-Wt-To-R1-L7279_S1


title: 4C-FGF9-Forebrain-E15-Wt-To

data_processing:
Raw reads of 4C-seq experiments were pre-processed and mapped to the reference genome talOcc4 using BWA (Li, 2009). Subsequently all reads were summarized and coverage normalized (RPM) for each fragment created by neighboring restriction enzyme sites.
For data visualization, the viewpoint and flanking fragments 1.5kb up- and downstream were removed and a window of 10 fragments was used to smooth the data.
Genome_build: talOcc4

source_name: Forebrain

extract_protocol:
4C-seq libraries were prepared following the published protocol (van de Werken et al., 2012). NlaIII was used for the first digestion, and DpnII was used in the second digestion. For each viewpoint, a total of 1.6 mg of each library was amplified by PCR with the following primers: Sall1-4C-Fwd:TCAGTGGGCTGACATTTTA, Sall1-4C-Rev: TCAGTGGGCTGACATTTTA, Fgf9-4C-Fwd:CAAAAGAAACTTCCAAAACC, Fgf9-4C-Rev:CAAAAGAAACTTCCAAAACC
Samples were sequenced with Ilumina Hi-Seq technology according to standard procedures.
4C-seq

treatment_protocol: Embryonic tissues, including limbs and forebrain, were disaggregated with trypsine, filter with a cell strainer to get single cell suspension and fixed in 2% FA.

value: Embryonic day 15

tag: viewpoint

value: FGF9 TSS


title: orco 12


title: H3K27me3-TestisPortion-P7-Wt-To-R2-L6093

description: To_Histones_segmentation_TestisPortion.bed

data_processing:
Each ChIPseq sample was mapped to the talOcc4 genome using STAR v2.6.1d (Dobin et al., 2013). In order to force an unspliced end-to-end alignment of the 50bp reads and to report only unique alignments the following options were applied: --alignEndsType EndToEnd   alignIntronMax 1   outFilterMatchNminOverLread 0.94 --outFilterMismatchNmax 5 --outFilterMultimapNmax 1.
Histone modification data was segmented genome-wide and classified into 7 modification states by applying the tool EpiCseg (Mammana et al., 2015).  If a state was supported by <50 regions the respective genomic regions were excluded from segmentation and EpiCseg was rerun.
Genome_build: talOcc4, mm9

extract_protocol:
Chromatin immunoprecipitations were performed using the iDeal ChIP-seq kit for Histones (Cat. No. C01010051) according guidelines.
Whole fixed gonads were sonicated with a Bioruptor (45 cycles 30sec on, 30 sec off at high power) in the shearing buffer from the kit. 5 mg of chromatin was used in each immunoprecipitation with 1mg of the following specific histones antibodies: anti-H3K4me3, Millipore, cat. No. 07-473; anti-H3K4me1, Diagenode, cat. No. C15410037; anti-H3K27ac, Diagenode, cat. No. C15410174; anti-H3K27me3, Millipore, cat. No. 07-449. Libraries were prepared using the Nextera adaptors and sequenced

treatment_protocol: Gonads from P7 mole animals and from E13.5 mouse embryos were fixed at 1% FA, snap freeze and store at -80°C.


title: H3K27me3-TestisPortion-P7-Wt-To-R1-L6092


title: H3K27me3-Testis-P7-Wt-To-R2-L6081


title: H3K27me3-OvaryPortion-P7-Wt-To-R2-L6087

description: To_Histones_segmentation_OvaryPortion.bed

source_name: Ovarian part of the ovotestis


title: H3K27me3-OvaryPortion-P7-Wt-To-R1-L6086


title: H3K27me3-Testis-P7-Wt-To-R1-L6080


title: orco 6


title: RNA-Testis_Adult_To-R2

data_processing:
For all samples 50bp paired-end reads were mapped against the respective genome (talOcc4, mm9) using STAR v2.6.1d (Dobin et al., 2013) with the following options: --chimSegmentMin 10   alignIntronMin 20   outFilterMismatchNoverReadLmax 0.05 --outFilterMismatchNmax 10 --outFilterMultimapNmax 5.
Genome_build: talOcc4, mm9

extract_protocol:
RNA was extracted from the gonads using the RNeasy kit from Qiagen
RNA libraries were prepared for sequencing using standard Illumina protocols

treatment_protocol: Gonads were disected rapidly after the animal was sacrified and then preserved in RNA later until the extraction

value: Adult


title: RNA-TestisPart_Adult_To-R2


title: RNA-OvaryPart_Adult_To-R1


title: orco 2


title: RNA-OvaryPart_Adult_To-R2


title: RNA-Testis_Adult_To-R1


title: RNA-TestisPart_Adult_To-R1


title: RNA-Testis_P7_To-R2


title: RNA-Testis_P7_To-R1


title: RNA-Testis_P7_To-R3


title: RNA-TestisPart_P7_To-R2


title: RNA-TestisPart_P7_To-R3


title: ATAC-OvaryPortion-P7-Wt-To-R2-L7298_S2


title: ATAC-OvaryPortion-P7-Wt-To-R1-L7297_S1


title: RNA-OvaryPart_P7_To-R3


title: RNA-TestisPart_P7_To-R1


title: RNA-OvaryPart_P7_To-R1


title: RNA-OvaryPart_P7_To-R2


title: RNA-Testis_E15_To-R2


title: RNA-Ovotestis_E15_To-R1

source_name: Ovotestis


title: RNA-Ovotestis_E15_To-R2


title: 4C-FGF9-Hindlimb-E15-Wt-To

source_name: Hindlimb


title: HiC from adult ovotestis

data_processing:
Raw reads from sequenced Hi-C libraries were processed using the Juicer pipeline v1.5.6., which was set up with the short read aligner BWA 0.7.17. The four replicates were processed independently and combined afterwards by merging the filtered and deduplicated read-pairs.
Genome_build: talOcc4

extract_protocol:
Five million cells were used to generate each library of Hi-C as previously described (Rao 2014).
One library with four technical replicates were generated following the in-situ Hi-C protocol from Rao 2014 without modifications.

treatment_protocol: Ovostestis from adult individuals were disaggregated with trypsine, filter with a cell strainer to obtain single cell suspension and fixed in 2% formaldehyde.

value: Gonads


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H9_ad010

value: CA

value: male


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H8_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H8_ad010


title: yahB_2

first: Ye

last: Gao

address: 9500 Gilman Dr.

data_processing:
The base calling was done using Illumina’s Real-Time Analysis (RTA) v2.7.7; data was converted to fastq and demultiplexed using Illumina’s bcl2fastq2 Conversion Software v2.20;
Sequence reads generated from ChIP-exo were mapped onto the each reference genome using bowtie with default options to generate SAM output files
MACE program was used to define peak candidates from biological duplicates for each experimental condition with sequence depth normalization.
To reduce false-positive peaks, manual curation was performed based on the signal-to-noise(S/N) ratio value.
The calculation of S/N ratio resembles the way to calculate ChIP-chip peak intensity where IP signal was divided by Mock signal. Then, each peak was assigned to the nearest gene.
Genome_build: MG1655 =  NC_000913.2
Supplementary_files_format_and_content: Tab-delimited text files in gff format which has 8 columns: sequence id, source(empty), feature (+/- strand), start position, end position, intensity score, strand(+/-), frame(.), attribute(.).

organism: Escherichia coli

source_name: bacteria cells

growth_protocol: glycerol stocks of E. coli myc-tagged strains were inoculated into M9 minimal media with 0.2% (w/v) glucose or other medium. The culture was incubated at 37 degree overnight with agitation, and then was used to inoculate the fresh media (1/200 dilution). The volume of the fresh media was 150 mL for each biological replicate. The fresh culture was incubated at 37 degree with agitation to the mid-log phase (OD600 ≈ 0.5).

extract_protocol:
Lysates were clarified from sonicated uncharacterized transcripton factors-DNA complexes were isolated with myc antibody.
ChIP-exo experiment was performed following the procedures:  to identify each TF candidate binding maps in vivo, we isolated the DNA bound to each TF candidate from formaldehyde cross-linked E. coli cells by chromatin immunoprecipitation (ChIP) with the specific antibodies that specifically recognizes myc tag (9E10, Santa Cruz Biotechnology), and Dynabeads Pan Mouse IgG magnetic beads (Invitrogen) followed by stringent washings as described previously. ChIP materials (chromatin-beads) were used to perform on-bead enzymatic reactions of the ChIP-exo method. Briefly, the sheared DNA of chromatin-beads was repaired by the NEBNext End Repair Module (New England Biolabs) followed by the addition of a single dA overhang and ligation of the first adaptor (5’-phosphorylated) using dA-Tailing Module (New England Biolabs) and NEBNext Quick Ligation Module (New England Biolabs), respectively. Nick repair was performed by using PreCR Repair Mix (New England Biolabs). Lambda exonuclease- and RecJf exonuclease-treated chromatin was eluted from the beads and the protein-DNA cross-link was reversed by overnight incubation at 65oC. RNAs- and Proteins-removed DNA samples were used to perform primer extension and second adaptor ligation with following modifications. The DNA samples incubated for primer extension as described previously were treated with dA-Tailing Module (New England Biolabs) and NEBNext Quick Ligation Module (New England Biolabs) for second adaptor ligation. The DNA sample purified by GeneRead Size Selection Kit (Qiagen) was enriched by polymerase chain reaction (PCR) using Phusion High-Fidelity DNA Polymerase (New England Biolabs). The amplified DNA samples were purified again by GeneRead Size Selection Kit (Qiagen) and quantified using Qubit dsDNA HS Assay Kit (Life Technologies). Quality of the DNA sample was checked by running Agilent High Sensitivity DNA Kit using Agilent 2100 Bioanalyzer (Agilent) before sequenced using HiSeq (Illumina) in accordance with the manufacturer’s instructions. Each modified step was also performed in accordance with the manufacturer’s instructions. ChIP-exo experiments were performed in biological duplicate.
ChIP-exo

treatment_protocol: For regulatory condition, M9 minimal media was supplemented with 1 mL trace element solution (100X) containing 1 g EDTA, 29 mg ZnSO4.7H2O, 198 mg MnCl2.4H2O, 254 mg CoCl2.6H2O, 13.4 mg CuCl2, and 147 mg CaCl2.

tag: myc-tagging

value: YahB myc-tagging cells

tag: chip-exo antibody

value: c-myc(9E10) mouse monoclonal IgG1,sc-40, lot#J2413, Santa Cruz Biotechnology


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H9_ad002

tag: strain


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H9_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H8_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H8_ad006


title: yqhC_2

value: YqhC myc-tagging cells


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H9_ad006


title: yahB_1


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H7_ad008


title: yqhC_1


title: yiaU_1

value: YiaU myc-tagging cells


title: yiaU_2


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H7_ad010


title: RNA-Testis_E15_To-R1

tag: genotype


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H7_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H7_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H7_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H6_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H6_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H6_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H7_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H7_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H6_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H9_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H6_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H6_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H5_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H2_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H6_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H3_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H3_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H2_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H11_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H5_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H5_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H3_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H5_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H2_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H2_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H11_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H3_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H2_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H2_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H11_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H11_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H11_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H11_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H2_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H11_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H10_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H10_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H11_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H3_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H5_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H2_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H10_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H10_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G9_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H10_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G9_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H10_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G9_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G8_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G9_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G7_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G8_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G8_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G8_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G7_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G7_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G7_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G7_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G7_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G6_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G8_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G7_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G6_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_H10_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G6_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G6_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G9_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G6_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G9_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G9_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G6_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G6_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G5_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G5_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G4_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G4_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G5_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G5_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G4_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G4_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G6_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G3_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G7_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G5_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G3_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G3_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G4_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G3_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G3_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G2_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G3_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G2_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G2_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G2_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G2_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G5_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G5_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G3_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G2_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G12_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G12_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G12_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G11_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G12_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G4_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G5_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G3_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G11_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G11_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G11_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G11_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G11_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G11_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G2_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G12_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G12_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G10_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G10_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G2_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G12_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G10_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G10_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G10_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G1_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G11_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G1_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G1_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G1_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G1_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G1_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G1_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F9_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F9_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F9_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F9_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F8_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F9_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F9_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F8_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F8_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F9_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F8_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F8_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F9_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F8_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G10_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F8_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F7_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F7_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F7_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F8_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F7_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F7_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F6_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F6_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F7_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F6_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F6_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F6_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F7_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F6_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F6_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F5_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F5_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F5_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G12_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F5_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F5_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F4_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F4_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F4_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F5_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F5_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F4_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F4_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F4_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F4_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F4_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F3_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F3_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F3_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F3_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F3_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F2_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F3_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F3_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F2_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F2_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F2_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F12_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F3_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F12_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F12_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F2_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F12_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F12_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F11_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F11_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F11_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F11_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F12_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F12_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F11_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F2_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F11_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F11_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F10_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F10_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F2_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F2_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F10_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F10_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F10_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F1_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F10_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F1_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E9_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E9_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F1_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E9_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F1_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E8_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E9_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E8_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E8_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E8_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E8_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E8_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E7_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E8_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E7_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E7_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E7_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E7_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_F1_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E7_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E9_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E6_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E9_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E8_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E6_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E5_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E5_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E6_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E6_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E5_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E5_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E6_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E5_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E5_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E4_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E7_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E4_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E5_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E4_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E4_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E4_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E4_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E3_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E3_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E3_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E4_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E3_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E3_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E3_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_G10_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E2_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E2_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E2_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E4_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E2_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E3_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E11_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E2_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E2_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E11_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E11_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E11_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E11_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E11_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E10_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E10_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E10_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E10_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E1_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E10_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E10_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E10_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E3_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E1_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E1_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E2_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E1_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E11_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E1_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D9_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E1_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D9_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D9_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D9_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D9_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D9_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D9_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D9_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D8_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D8_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D8_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D8_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D8_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D7_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E1_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D6_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D8_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D7_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D6_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D6_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D7_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D7_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D6_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D6_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D6_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D5_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D6_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D8_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_E1_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D5_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D5_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D5_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D5_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D4_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D4_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D4_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D4_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D4_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D3_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D3_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D3_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D3_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D3_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D4_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D3_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D2_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D3_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D2_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D2_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D2_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D2_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D12_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D2_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D12_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D4_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D5_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D5_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D12_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D12_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D12_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D3_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D4_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D12_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D12_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D11_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D11_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D11_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D11_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D10_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D11_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D10_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D10_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D11_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D10_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D10_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D1_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D10_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D10_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D1_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D1_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D1_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D1_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D1_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C9_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C9_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D11_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D12_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C9_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C9_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C9_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C9_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C8_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C8_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C8_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_D10_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C8_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C8_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C7_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C7_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C6_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C6_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C6_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C6_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C6_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C7_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C8_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C5_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C5_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C5_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C4_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C4_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C4_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C5_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C7_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C5_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C4_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C4_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C3_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C7_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C7_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C3_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C3_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C3_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C2_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C5_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C2_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C2_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C3_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C2_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C2_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C11_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C2_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C2_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C4_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C4_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C11_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C11_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C11_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C11_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C11_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C11_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C3_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C3_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C10_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C3_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C10_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C10_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C10_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C10_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C10_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C10_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C1_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C1_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C1_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C2_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C1_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C1_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C1_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C1_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_C1_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B9_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B9_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B9_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B9_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B8_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B8_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B9_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B8_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B8_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B8_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B9_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B8_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B9_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B8_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B7_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B7_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B7_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B7_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B7_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B7_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B7_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B7_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B6_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B6_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B6_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B6_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B6_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B6_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B6_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B6_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B5_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B5_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B5_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B5_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B5_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B4_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B4_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B5_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B5_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B4_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B4_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B4_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B4_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B3_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B4_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B3_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B3_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B3_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B2_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B3_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B3_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B3_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B2_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B2_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B12_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B12_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B2_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B2_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B12_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B2_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B12_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B12_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B12_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B11_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B10_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B10_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B11_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B11_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B11_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B12_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B12_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B11_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B10_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B2_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B10_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B10_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B1_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B10_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B1_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B1_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B1_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A9_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A9_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A9_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B1_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A9_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B11_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A9_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A8_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A8_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A8_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A8_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A8_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A8_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A7_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A7_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A7_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A7_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A7_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A6_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A6_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A7_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A7_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A6_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B1_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A6_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B1_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A7_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_B1_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A6_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A6_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A5_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A5_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A5_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A5_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A6_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A5_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A4_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A4_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A4_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A3_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A3_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A3_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A3_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A3_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A3_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A2_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A2_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A2_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A2_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A2_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A12_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A12_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A12_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A5_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A11_ad012


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A11_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A10_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A11_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A11_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A11_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A4_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A11_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A4_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A10_ad002


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A3_ad010


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A10_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A10_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A10_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A10_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A10_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A1_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A1_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A1_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A1_ad004


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A1_ad001


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A1_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H9_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H9_ad007


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A10_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H9_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H9_ad008


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A11_ad008


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H9_ad006


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H9_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H8_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H7_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H7_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H8_ad006


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H7_ad008


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H8_ad008


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H7_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H7_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H7_ad006


title: CEMBA190305_11E_3_CEMBA190305_11E_4_A1_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H7_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H6_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H6_ad008


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H7_ad001


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H6_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H6_ad006


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H6_ad001


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H5_ad008


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H6_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H5_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H5_ad001


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H5_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H8_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H4_ad008


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H4_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H4_ad006


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H4_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H4_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H4_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H4_ad001


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H3_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H2_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H3_ad008


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H2_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H3_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H3_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H2_ad008


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H3_ad001


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H2_ad006


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H2_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H12_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H12_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H5_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H12_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H12_ad001


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H12_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H12_ad006


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H11_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H12_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H11_ad008


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H11_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H11_ad006


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H11_ad001


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H11_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H11_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H10_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H10_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H10_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H10_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H10_ad008


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H10_ad001


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H2_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H2_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H2_ad001


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H10_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H1_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H1_ad008


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H1_ad006


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H1_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H1_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H1_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G9_ad008


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G9_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G8_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G9_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G9_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G8_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G8_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G8_ad001


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G8_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H10_ad006


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G7_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G7_ad008


title: CEMBA190305_11E_1_CEMBA190305_11E_2_H1_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G7_ad006


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G7_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G6_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G6_ad008


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G6_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G7_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G6_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G7_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G9_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G9_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G6_ad001


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G6_ad006


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G6_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G5_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G5_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G7_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G5_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G5_ad006


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G4_ad008


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G5_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G4_ad006


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G4_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G4_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G3_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G3_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G3_ad008


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G3_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G3_ad006


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G3_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G3_ad001


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G3_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G2_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G2_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G5_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G2_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G12_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G2_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G2_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G12_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G11_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G4_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G12_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G11_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G11_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G11_ad008


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G11_ad001


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G11_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G10_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G10_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G10_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G10_ad006


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G1_ad008


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G1_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G1_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G2_ad006


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G10_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G12_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G12_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G1_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G12_ad008


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G1_ad006


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G12_ad006


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G1_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G1_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G1_ad001


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F9_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F9_ad006


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F9_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F9_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F9_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F9_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G11_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F9_ad001


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F9_ad008


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G10_ad001


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F8_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F8_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F8_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F8_ad006


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F8_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F8_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F7_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F8_ad008


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F7_ad008


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F7_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F7_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F6_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F7_ad001


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F7_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F6_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F6_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F7_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F7_ad006


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F5_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F5_ad008


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F6_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F5_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F6_ad006


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F5_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F6_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F5_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F6_ad001


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F5_ad001


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F5_ad006


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F5_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F4_ad008


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F4_ad006


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F4_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F4_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F4_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F4_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F4_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F4_ad001


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F3_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F3_ad008


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F3_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F3_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F3_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F3_ad001


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F2_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F2_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_G10_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F12_ad008


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F2_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F12_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F12_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F12_ad001


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F2_ad001


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F12_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F12_ad006


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F2_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F2_ad008


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F12_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F12_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F11_ad001


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F11_ad006


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F11_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F10_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F11_ad008


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F10_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F10_ad008


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F10_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F10_ad001


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F10_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F2_ad006


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F2_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F1_ad012


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F10_ad006


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F11_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F1_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F1_ad006


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F1_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_E9_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F1_ad001


title: CEMBA190305_11E_1_CEMBA190305_11E_2_E9_ad007


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F1_ad008


title: Wild type crown root tip RNA-seq repeat2

city: Wuhan

first: shaoli

last: zhou

state: Hubei

address: shizishan street

department: National Key Laboratory of Crop Genetic Improvement

country: China

institute: Huazhong Agricultural University

description: Sample 4

data_processing:
Tophat (version 2.0.13) was used to align the clean tags filtered by Trimmomatic (version 0.32) to the reference genome of Niponbarre (version 7.0). Cufflink (version 2.2.1) suite was used for identification of differentially expressed genes.
Genome_build: RGAP version 7.0
Supplementary_files_format_and_content: tab-delimited text file containing differentially expressed gene information

organism: Oryza sativa Japonica Group

source_name: crown root tip

growth_protocol: Rice (Oryza sativa spp japonica) material for transformation of OsMAL1 RNAi vector was used in this study is from ‘ZhongHua11’ (ZH11) background. Seeds were surface-sterilized and geminated on MS medium (0.8% agar and 3% (w/v) sucrose) at 28℃ (in light) and 24℃ (in dark) with a 14-h-light/10-h-dark cycle. After germinated on agar medium for 5 days, RNA of root tips was  was isolated.

extract_protocol:
The totle RNA of rice root tip (3-5mm) was isolated using TRIzol (Invitrogen) according to the manufacturer’s instructions, purified with phenol chloroform mixture, and precipitated by 2 volume alcohol.
RNA libraries were prepared according to the Illumina RNA-seq library construction protocol strictly.  Briefly, 4 μg of total RNAs was used for mRNA purification. After first and second strand cDNA systhesis, index primers was added. Followed with low cycle PCR amplyfication and double-strand DNA purification.

tag: tissue

value: 5 day-after-germination crown root tip


title: CEMBA190305_11E_1_CEMBA190305_11E_2_E9_ad008


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F1_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F10_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_E9_ad006


title: Wild type crown root tip RNA-seq repeat1

description: Sample 3


title: MAL RNAi crown root tip RNA-seq repeat2

description: Sample 2


title: CEMBA190305_11E_1_CEMBA190305_11E_2_E9_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_E9_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_E9_ad001


title: CEMBA190305_11E_1_CEMBA190305_11E_2_E8_ad012


title: MAL RNAi crown root tip RNA-seq repeat1

description: Sample 1


title: muscle_WGBS_rep2

city: Darlinghurst

first: Ozren

last: Bogdanovic

state: NSW

address: 384 Victoria Street

department: Genomics and Epigenetics

country: Australia

institute: Garvan Institute of Medical Research

description: wild type

data_processing:
Reads were trimmed Trimmomatic (ILLUMINACLIP:TruSeq3-SE.fa:2:30:10 SLIDINGWINDOW:5:20 LEADING:3 TRAILING:3 CROP:140 HEADCROP:5) (Bolger et al. 2014), and mapped using WALT (-m 5 -t 20 -N 10000000) (Chen et al. 2016) onto the bisulfite-converted GRCz11 reference (UCSC) containing λ added as separate chromosomes. The resulting alignments in BAM format were processed with CGmapTools (Guo et al. 2018) (convert bam2cgmap) to obtain methylation calls. ATCGmap files were parsed to discard the CH positions that showed evidence of a CG position in the reads discordant with the reference genome CH annotation.
Genome_build: GRCz11
Supplementary_files_format_and_content: bigwig files for visualization with UCSC genome browser containing methylation value as mC/C

organism: Danio rerio

source_name: Adult

growth_protocol: Adult wild type (AB-Tubingen) Danio rerio (zebrafish) were bred in a 1 male:1 female ratio. Embryos were collected 0 hours post-fertilisation (hpf) and incubated in 1X E3 medium (0.03% NaCl, 0.005% CaCl2, 0.0013% KCl, 99.9557% H2O, 0.008% H14MgO11S) for 24 hours at 28.5°C.

extract_protocol:
For WGBS, 500ng of zebrafish gDNA and spiked in 0.025ng of unmethylated lambda phage DNA (Promega, Madison, WI, USA) was sonicated to an average insert size of 300bp. Sonicated DNA was end repaired followed by ligation of adapters to DNA overnight using NEXTFLEX Bisulfite-Seq Barcodes (PerkinElmer, Waltham, MA, USA). DNA was bisulfite converted using EZ DNA Methylation Gold Kit (Zymo Research,Irvine,CA,USA ) according to manufacturer instructions. Library amplification was performed with KAPA HiFi HotStart Uracil+ DNA polymerase (Kapa Biosystems, Woburn, MA), using 8 cycles of amplification. Library concentration was quantified through qPCR using KAPA Library Quantification Kit (Sigma-Aldrich, St. Louis, MO, USA) according to manufacturer instructions. The combined libraries with 15% PhiX spike-in was sequenced on the Illumina HiSeq 10X platform using 150bp paired-end sequencing (high output mode).
embryos/tissues were snap frozen in liquid nitrogen and stored at -80°C prior to DNA extraction. Genomic DNA (gDNA) was extracted using the QIAGEN DNeasy Blood & Tissue Kit (QIAGEN, Chadstone, VIC, Australia) according to manufacturer instructions in two biological replicates.

treatment_protocol: KO microinjection mixtures where made by combining 1000 ng of gRNA with 1 μl EnGene CAS9 NLS, S Pyogenes  (New England BioLabs, Ipswich, MA, USA) and 1.5 μl KCl (1M).  500pL of micoinejection mixtures were injected into single cell stage embryos.

value: muscle

value: Mixed AB and Tubingen

value: wild type


title: CEMBA190305_11E_1_CEMBA190305_11E_2_E8_ad008


title: CEMBA190305_11E_1_CEMBA190305_11E_2_E8_ad007


title: skin_WGBS_rep1

description: wild type

value: skin


title: intestine_WGBS_rep2

description: wild type

value: intestine


title: CEMBA190305_11E_1_CEMBA190305_11E_2_F1_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_E9_ad012


title: muscle_WGBS_rep1

description: wild type


title: CEMBA190305_11E_1_CEMBA190305_11E_2_E8_ad006


title: intestine_WGBS_rep1

description: wild type


title: CEMBA190305_11E_1_CEMBA190305_11E_2_E8_ad010


title: heart_WGBS_rep2

description: wild type

value: heart


title: CEMBA190305_11E_1_CEMBA190305_11E_2_E8_ad001


title: CEMBA190305_11E_1_CEMBA190305_11E_2_E8_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_E8_ad004


title: CEMBA190305_11E_1_CEMBA190305_11E_2_E7_ad012


title: heart_WGBS_rep1

description: wild type


title: dnmt3ba_cKO_72hpf_WGBS_rep1

description: CRISPR-Cas9 injected F0 zebrafish with mosaic KO levels

source_name: 72hpf embryos

value: whole embryos

value: dnmt3ba CRIPSR-CAS9 injected F0


title: CEMBA190305_11E_1_CEMBA190305_11E_2_E7_ad010


title: CEMBA190305_11E_1_CEMBA190305_11E_2_E7_ad008


title: WT_72hpf_WGBS_rep2

description: control


title: CEMBA190305_11E_1_CEMBA190305_11E_2_E7_ad006


title: skin_WGBS_rep2

description: wild type


title: CEMBA190305_11E_1_CEMBA190305_11E_2_E7_ad002


title: CEMBA190305_11E_1_CEMBA190305_11E_2_E7_ad004


title: WT_72hpf_WGBS_rep1

description: control


title: dnmt3bb3_cKO_48hpf_RRBS_rep1

source_name: 48hpf embryos

extract_protocol:
RRBS library construction was performed using the restriction enzyme BccI and SspI (New England BioLabs, Ipswich, MA, USA) and 0.025ng of unmethylated lambda phage DNA (Promega, Madison, WI, USA) was used as a spike-in control to determine the efficiency of bisufite conversion. Briefly, 500ng of zebrafish gDNA was digested for 2 hours with 10U BccI and 10U SspI (New England BioLabs, Ipswich, MA, USA). The 5’ overhangs of the digested DNA were filled in and A-tailed using klenow fragment exo-  (New England BioLabs, Ipswich, MA, USA) before ligation of adapters to DNA overnight using NEXTFLEX Bisulfite-Seq Barcodes (PerkinElmer, Waltham, MA, USA). DNA was bisulfite converted using EZ DNA Methylation Gold Kit (Zymo Research,Irvine,CA,USA ) according to manufacturer instructions. Library amplification was performed with KAPA HiFi HotStart Uracil+ DNA polymerase (Kapa Biosystems, Woburn, MA), using 13 cycles of amplification. Library concentration was quantified through qPCR using KAPA Library Quantification Kit (Sigma-Aldrich, St. Louis, MO, USA) according to manufacturer instructions. The combined libraries with 15% PhiX spike-in was sequenced on the Illumina HiSeq 10X platform using 150bp paired-end sequencing (high output mode).
embryos/tissues were snap frozen in liquid nitrogen and stored at -80°C prior to DNA extraction. Genomic DNA (gDNA) was extracted using the QIAGEN DNeasy Blood & Tissue Kit (QIAGEN, Chadstone, VIC, Australia) according to manufacturer instructions in two biological replicates.

value: dnmt3bb.3 CRIPSR-CAS9 injected F0


title: CEMBA190305_11E_1_CEMBA190305_11E_2_E6_ad010