title: Subject 14, Hour 060

description: Gene expression profile from peripheral blood of subject 14, hour 060


title: Subject 15, Hour 045

status: Public on Jul 12 2011

type: RNA

city: Ann Arbor

first: Yongsheng

last: Huang

state: MI

address: 100 Washtenaw Ave

department: Bioinformatics

country: USA

institute: University of Michigan

description: Gene expression profile from peripheral blood of subject 15, hour 045

hyb_protocol: Following fragmentation, cRNA were hybridized for on GeneChip Human Genome U133A 2.0 Array.\

scan_protocol: GeneChips were scanned using the Affymetrix GeneArray Scanner.

data_processing: The data were analyzed with robust multi-array average (RMA) normalization using default background adjustment and quantile normalization settings. A custom Chip Definition File (CDF) version 10 for more accurate probe mapping to genome (Nucleic Acid Research 33 (20), e175)

label: biotin

molecule: total RNA

organism: Homo sapiens

label_protocol: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).

extract_protocol: RNA was extracted at Expression Analysis (Durham, NC) from whole blood using the PAXgene™ 96 Blood RNA Kit (PreAnalytiX, Valencia, CA) employing the manufacturer's recommended protocol. While whole blood RNA is initially extracted, a secondary procedure (B-globin reduction) was then employed to remove the contribution of red blood cell (RBC) RNA to the total RNA. A set of four peptide nucleic acid (PNA) oligomers whose sequences are complementary to the 3’ portions of the alpha and beta hemoglobin RNA transcripts were added to reduce globin RNA transcription due to RBC. The inhibition of globin cDNA synthesis dramatically reduces the relative amount of anti-sense, biotin-labeled cRNA corresponding to the hemoglobin transcripts.

tag: tissue

value: peripheral blood

value: Hour 045

value: Symptomatic


title: Subject 13, Hour 101

description: Gene expression profile from peripheral blood of subject 13, hour 101

value: Hour 101


title: Subject 14, Hour 108

description: Gene expression profile from peripheral blood of subject 14, hour 108

value: Hour 108

value: Asymptomatic


title: Subject 14, Hour 00

description: Gene expression profile from peripheral blood of subject 14, hour 00

source_name: peripheral blood of subject 14, hour 00

value: Hour 00


title: Subject 14, Hour 021

description: Gene expression profile from peripheral blood of subject 14, hour 021

value: Hour 021


title: Subject 14, Hour 005

description: Gene expression profile from peripheral blood of subject 14, hour 005

value: Hour 005


title: Subject 14, Hour 029

description: Gene expression profile from peripheral blood of subject 14, hour 029

value: Hour 029


title: Subject 13, Hour 093

description: Gene expression profile from peripheral blood of subject 13, hour 093

value: Hour 093


title: Subject 13, Hour 108

description: Gene expression profile from peripheral blood of subject 13, hour 108


title: Subject 13, Hour 060

description: Gene expression profile from peripheral blood of subject 13, hour 060

value: Hour 060


title: Subject 13, Hour 053

description: Gene expression profile from peripheral blood of subject 13, hour 053

value: Hour 053


title: Subject 13, Hour 045

description: Gene expression profile from peripheral blood of subject 13, hour 045


title: Subject 14, Baseline

description: Gene expression profile from peripheral blood of subject 14, baseline

value: Baseline


title: Subject 13, Hour 029

description: Gene expression profile from peripheral blood of subject 13, hour 029


title: Subject 13, Hour 021

description: Gene expression profile from peripheral blood of subject 13, hour 021


title: Subject 13, Hour 084

description: Gene expression profile from peripheral blood of subject 13, hour 084

value: Hour 084


title: Subject 13, Hour 00

description: Gene expression profile from peripheral blood of subject 13, hour 00

source_name: peripheral blood of subject 13, hour 00


title: Subject 12, Hour 108

description: Gene expression profile from peripheral blood of subject 12, hour 108


title: Subject 13, Hour 005

description: Gene expression profile from peripheral blood of subject 13, hour 005


title: Subject 13, Hour 077

description: Gene expression profile from peripheral blood of subject 13, hour 077

value: Hour 077


title: Subject 12, Hour 093

description: Gene expression profile from peripheral blood of subject 12, hour 093


title: Subject 12, Hour 101

description: Gene expression profile from peripheral blood of subject 12, hour 101


title: Subject 12, Hour 084

description: Gene expression profile from peripheral blood of subject 12, hour 084


title: Subject 12, Hour 060

description: Gene expression profile from peripheral blood of subject 12, hour 060


title: Subject 12, Hour 077

description: Gene expression profile from peripheral blood of subject 12, hour 077


title: Subject 12, Hour 069

description: Gene expression profile from peripheral blood of subject 12, hour 069

value: Hour 069


title: Subject 12, Hour 045

description: Gene expression profile from peripheral blood of subject 12, hour 045

value: peripheral blood


title: Subject 12, Hour 036

description: Gene expression profile from peripheral blood of subject 12, hour 036

value: Hour 036


title: Subject 12, Hour 029

description: Gene expression profile from peripheral blood of subject 12, hour 029

value: Symptomatic


title: Subject 12, Hour 012

description: Gene expression profile from peripheral blood of subject 12, hour 012

value: Hour 012


title: Subject 12, Hour 005

description: Gene expression profile from peripheral blood of subject 12, hour 005


title: Subject 12, Hour 021

description: Gene expression profile from peripheral blood of subject 12, hour 021


title: Subject 11, Hour 101

description: Gene expression profile from peripheral blood of subject 11, hour 101

value: Hour 101


title: Subject 11, Hour 084

description: Gene expression profile from peripheral blood of subject 11, hour 084

value: Asymptomatic


title: Subject 11, Hour 077

description: Gene expression profile from peripheral blood of subject 11, hour 077


title: Subject 11, Hour 108

description: Gene expression profile from peripheral blood of subject 11, hour 108

value: Hour 108


title: Subject 11, Hour 069

description: Gene expression profile from peripheral blood of subject 11, hour 069


title: Subject 11, Hour 060

description: Gene expression profile from peripheral blood of subject 11, hour 060


title: Subject 11, Hour 045

description: Gene expression profile from peripheral blood of subject 11, hour 045

value: Hour 045


title: Subject 11, Hour 053

description: Gene expression profile from peripheral blood of subject 11, hour 053


title: Subject 11, Hour 036

description: Gene expression profile from peripheral blood of subject 11, hour 036


title: Subject 11, Hour 021

description: Gene expression profile from peripheral blood of subject 11, hour 021

value: Hour 021


title: Subject 12, Hour 053

description: Gene expression profile from peripheral blood of subject 12, hour 053


title: SC.LT34F.BM#3

status: Public on Sep 07 2011

city: Boston

first: Liang

last: Yang

state: MA

address: 77 Avenue Louis Pasteur

department: Pathology

institute: Harvard Medical School

description: SC.LT34F.BM#3, CD34-FLK2- LT-HSC CD34-Flk2-Lin-c-kit+Sca1+

hyb_protocol: Affy 1.0 ST Hyb Kit

scan_protocol: Affymetrix GeneChip Scanner

data_processing: The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.

label: Biotin

organism: Mus musculus

source_name: Bone marrow

label_protocol: Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit

extract_protocol: Whole Bone Marrow from Legs+Hips+Arms crushed and filtered, Enrich cKit (Milteny CD117 beads) and AutoMax, double sorted

tag: age

value: 9 weeks

tag: exclusion markers

value: PI

tag: genetic background

tag: phenotype markers

value: Lineage- cKit+ Sca-1+ flk2- CD34-

tag: separation method

value: dissection/trimming

tag: Sex

value: male


title: Subject 11, Hour 012

description: Gene expression profile from peripheral blood of subject 11, hour 012


title: Subject 11, Hour 005

description: Gene expression profile from peripheral blood of subject 11, hour 005

value: Hour 005


title: Subject 12, Baseline

description: Gene expression profile from peripheral blood of subject 12, baseline

value: Baseline


title: Subject 12, Hour 00

description: Gene expression profile from peripheral blood of subject 12, hour 00

source_name: peripheral blood of subject 12, hour 00

value: Hour 00


title: Subject 14, Hour 012

description: Gene expression profile from peripheral blood of subject 14, hour 012

value: peripheral blood


title: Subject 11, Hour 093

description: Gene expression profile from peripheral blood of subject 11, hour 093

value: Hour 093


title: Subject 11, Hour 029

description: Gene expression profile from peripheral blood of subject 11, hour 029

value: Hour 029


title: Subject 10, Hour 108

description: Gene expression profile from peripheral blood of subject 10, hour 108

value: Symptomatic


title: Subject 10, Hour 101

description: Gene expression profile from peripheral blood of subject 10, hour 101

value: Hour 101


title: Subject 11, Baseline

description: Gene expression profile from peripheral blood of subject 11, baseline

value: Asymptomatic


title: Subject 10, Hour 060

description: Gene expression profile from peripheral blood of subject 10, hour 060

value: Hour 060


title: Subject 10, Hour 084

description: Gene expression profile from peripheral blood of subject 10, hour 084

value: Hour 084


title: Subject 10, Hour 077

description: Gene expression profile from peripheral blood of subject 10, hour 077

value: Hour 077


title: Subject 10, Hour 045

description: Gene expression profile from peripheral blood of subject 10, hour 045

value: Hour 045


title: Subject 10, Hour 093

description: Gene expression profile from peripheral blood of subject 10, hour 093


title: Subject 10, Hour 029

description: Gene expression profile from peripheral blood of subject 10, hour 029


title: Subject 10, Hour 053

description: Gene expression profile from peripheral blood of subject 10, hour 053

value: Hour 053


title: Subject 10, Hour 069

description: Gene expression profile from peripheral blood of subject 10, hour 069

value: Hour 069


title: Subject 10, Hour 012

description: Gene expression profile from peripheral blood of subject 10, hour 012

value: Hour 012


title: Subject 10, Hour 005

description: Gene expression profile from peripheral blood of subject 10, hour 005


title: Subject 09, Hour 084

description: Gene expression profile from peripheral blood of subject 09, hour 084


title: Subject 09, Hour 093

description: Gene expression profile from peripheral blood of subject 09, hour 093


title: Subject 09, Hour 101

description: Gene expression profile from peripheral blood of subject 09, hour 101


title: Subject 09, Hour 077

description: Gene expression profile from peripheral blood of subject 09, hour 077


title: Subject 09, Hour 069

description: Gene expression profile from peripheral blood of subject 09, hour 069


title: Subject 09, Hour 060

description: Gene expression profile from peripheral blood of subject 09, hour 060


title: Subject 10, Hour 036

description: Gene expression profile from peripheral blood of subject 10, hour 036

value: Hour 036


title: Subject 09, Hour 053

description: Gene expression profile from peripheral blood of subject 09, hour 053


title: Subject 10, Hour 021

description: Gene expression profile from peripheral blood of subject 10, hour 021

value: Hour 021


title: Subject 09, Hour 045

description: Gene expression profile from peripheral blood of subject 09, hour 045


title: Subject 09, Hour 029

description: Gene expression profile from peripheral blood of subject 09, hour 029


title: Subject 10, Hour 00

description: Gene expression profile from peripheral blood of subject 10, hour 00

source_name: peripheral blood of subject 10, hour 00


title: Subject 09, Hour 036

description: Gene expression profile from peripheral blood of subject 09, hour 036


title: Subject 10, Baseline

description: Gene expression profile from peripheral blood of subject 10, baseline

value: Baseline


title: Subject 09, Hour 021

description: Gene expression profile from peripheral blood of subject 09, hour 021


title: Subject 09, Hour 012

description: Gene expression profile from peripheral blood of subject 09, hour 012


title: Subject 09, Baseline

description: Gene expression profile from peripheral blood of subject 09, baseline


title: Subject 13, Hour 069

description: Gene expression profile from peripheral blood of subject 13, hour 069


title: Subject 08, Hour 093

description: Gene expression profile from peripheral blood of subject 08, hour 093


title: Subject 08, Hour 084

description: Gene expression profile from peripheral blood of subject 08, hour 084


title: Subject 08, Hour 108

description: Gene expression profile from peripheral blood of subject 08, hour 108

value: Hour 108


title: Subject 08, Hour 077

description: Gene expression profile from peripheral blood of subject 08, hour 077

value: peripheral blood


title: Subject 09, Hour 00

description: Gene expression profile from peripheral blood of subject 09, hour 00

source_name: peripheral blood of subject 09, hour 00

value: Hour 00


title: Subject 08, Hour 060

description: Gene expression profile from peripheral blood of subject 08, hour 060


title: Subject 08, Hour 00

description: Gene expression profile from peripheral blood of subject 08, hour 00

source_name: peripheral blood of subject 08, hour 00


title: Subject 07, Hour 108

description: Gene expression profile from peripheral blood of subject 07, hour 108


title: Subject 08, Hour 005

description: Gene expression profile from peripheral blood of subject 08, hour 005

value: Hour 005


title: Subject 08, Baseline

description: Gene expression profile from peripheral blood of subject 08, baseline

value: Symptomatic


title: Subject 08, Hour 029

description: Gene expression profile from peripheral blood of subject 08, hour 029

value: Hour 029


title: Subject 08, Hour 012

description: Gene expression profile from peripheral blood of subject 08, hour 012


title: Subject 08, Hour 069

description: Gene expression profile from peripheral blood of subject 08, hour 069


title: Subject 07, Hour 084

description: Gene expression profile from peripheral blood of subject 07, hour 084


title: Subject 08, Hour 053

description: Gene expression profile from peripheral blood of subject 08, hour 053


title: Subject 07, Hour 101

description: Gene expression profile from peripheral blood of subject 07, hour 101

value: Hour 101


title: Subject 09, Hour 108

description: Gene expression profile from peripheral blood of subject 09, hour 108

value: Asymptomatic


title: Subject 08, Hour 036

description: Gene expression profile from peripheral blood of subject 08, hour 036


title: Subject 08, Hour 101

description: Gene expression profile from peripheral blood of subject 08, hour 101


title: Subject 07, Hour 093

description: Gene expression profile from peripheral blood of subject 07, hour 093

value: Hour 093


title: Subject 08, Hour 045

description: Gene expression profile from peripheral blood of subject 08, hour 045


title: Subject 07, Hour 060

description: Gene expression profile from peripheral blood of subject 07, hour 060

value: Hour 060


title: Subject 07, Hour 053

description: Gene expression profile from peripheral blood of subject 07, hour 053


title: Subject 07, Hour 045

description: Gene expression profile from peripheral blood of subject 07, hour 045

value: Hour 045


title: Subject 07, Hour 021

description: Gene expression profile from peripheral blood of subject 07, hour 021


title: Subject 07, Hour 012

description: Gene expression profile from peripheral blood of subject 07, hour 012


title: Subject 07, Hour 029

description: Gene expression profile from peripheral blood of subject 07, hour 029


title: Subject 07, Hour 005

description: Gene expression profile from peripheral blood of subject 07, hour 005


title: Subject 07, Hour 00

description: Gene expression profile from peripheral blood of subject 07, hour 00

source_name: peripheral blood of subject 07, hour 00


title: Subject 07, Hour 036

description: Gene expression profile from peripheral blood of subject 07, hour 036


title: Subject 07, Baseline

description: Gene expression profile from peripheral blood of subject 07, baseline


title: Subject 07, Hour 077

description: Gene expression profile from peripheral blood of subject 07, hour 077

value: Hour 077


title: Subject 07, Hour 069

description: Gene expression profile from peripheral blood of subject 07, hour 069

value: Hour 069


title: Subject 11, Hour 00

description: Gene expression profile from peripheral blood of subject 11, hour 00

source_name: peripheral blood of subject 11, hour 00


title: Subject 06, Hour 101

description: Gene expression profile from peripheral blood of subject 06, hour 101


title: Subject 06, Hour 108

description: Gene expression profile from peripheral blood of subject 06, hour 108


title: Subject 06, Hour 036

description: Gene expression profile from peripheral blood of subject 06, hour 036


title: Subject 06, Hour 060

description: Gene expression profile from peripheral blood of subject 06, hour 060


title: Subject 06, Hour 093

description: Gene expression profile from peripheral blood of subject 06, hour 093


title: Subject 06, Hour 077

description: Gene expression profile from peripheral blood of subject 06, hour 077


title: Subject 06, Hour 069

description: Gene expression profile from peripheral blood of subject 06, hour 069


title: Subject 05, Hour 108

description: Gene expression profile from peripheral blood of subject 05, hour 108


title: Subject 06, Hour 029

description: Gene expression profile from peripheral blood of subject 06, hour 029


title: Subject 06, Hour 012

description: Gene expression profile from peripheral blood of subject 06, hour 012

value: Hour 012


title: Subject 06, Baseline

description: Gene expression profile from peripheral blood of subject 06, baseline


title: Subject 05, Hour 069

description: Gene expression profile from peripheral blood of subject 05, hour 069


title: Subject 13, Hour 012

description: Gene expression profile from peripheral blood of subject 13, hour 012


title: Subject 06, Hour 00

description: Gene expression profile from peripheral blood of subject 06, hour 00

source_name: peripheral blood of subject 06, hour 00


title: Subject 06, Hour 005

description: Gene expression profile from peripheral blood of subject 06, hour 005


title: Subject 06, Hour 021

description: Gene expression profile from peripheral blood of subject 06, hour 021


title: Subject 05, Hour 077

description: Gene expression profile from peripheral blood of subject 05, hour 077


title: Subject 05, Hour 036

description: Gene expression profile from peripheral blood of subject 05, hour 036

value: Hour 036


title: Subject 06, Hour 045

description: Gene expression profile from peripheral blood of subject 06, hour 045


title: Subject 05, Hour 045

description: Gene expression profile from peripheral blood of subject 05, hour 045


title: Subject 05, Hour 029

description: Gene expression profile from peripheral blood of subject 05, hour 029


title: Subject 05, Hour 012

description: Gene expression profile from peripheral blood of subject 05, hour 012


title: Subject 06, Hour 084

description: Gene expression profile from peripheral blood of subject 06, hour 084

value: Hour 084


title: Subject 05, Hour 021

description: Gene expression profile from peripheral blood of subject 05, hour 021

value: Hour 021


title: Subject 05, Hour 005

description: Gene expression profile from peripheral blood of subject 05, hour 005


title: Subject 05, Hour 084

description: Gene expression profile from peripheral blood of subject 05, hour 084


title: Subject 05, Hour 101

description: Gene expression profile from peripheral blood of subject 05, hour 101


title: Subject 05, Hour 093

description: Gene expression profile from peripheral blood of subject 05, hour 093


title: Subject 05, Hour 00

description: Gene expression profile from peripheral blood of subject 05, hour 00

source_name: peripheral blood of subject 05, hour 00


title: Subject 04, Hour 108

description: Gene expression profile from peripheral blood of subject 04, hour 108

value: Hour 108


title: Subject 05, Baseline

description: Gene expression profile from peripheral blood of subject 05, baseline

value: peripheral blood

value: Baseline


title: Subject 04, Hour 101

description: Gene expression profile from peripheral blood of subject 04, hour 101


title: Subject 06, Hour 053

description: Gene expression profile from peripheral blood of subject 06, hour 053

value: Hour 053


title: Subject 05, Hour 060

description: Gene expression profile from peripheral blood of subject 05, hour 060

value: Symptomatic


title: Subject 05, Hour 053

description: Gene expression profile from peripheral blood of subject 05, hour 053


title: Subject 04, Hour 093

description: Gene expression profile from peripheral blood of subject 04, hour 093


title: Subject 04, Hour 084

description: Gene expression profile from peripheral blood of subject 04, hour 084


title: Subject 04, Hour 069

description: Gene expression profile from peripheral blood of subject 04, hour 069


title: Subject 04, Hour 012

description: Gene expression profile from peripheral blood of subject 04, hour 012


title: Subject 04, Hour 053

description: Gene expression profile from peripheral blood of subject 04, hour 053


title: Subject 04, Hour 077

description: Gene expression profile from peripheral blood of subject 04, hour 077


title: Subject 04, Hour 021

description: Gene expression profile from peripheral blood of subject 04, hour 021


title: Subject 04, Hour 036

description: Gene expression profile from peripheral blood of subject 04, hour 036


title: Subject 04, Baseline

description: Gene expression profile from peripheral blood of subject 04, baseline


title: Subject 03, Hour 101

description: Gene expression profile from peripheral blood of subject 03, hour 101


title: Subject 03, Hour 108

description: Gene expression profile from peripheral blood of subject 03, hour 108


title: Subject 04, Hour 00

description: Gene expression profile from peripheral blood of subject 04, hour 00

source_name: peripheral blood of subject 04, hour 00

value: Hour 00


title: Subject 03, Hour 093

description: Gene expression profile from peripheral blood of subject 03, hour 093


title: Subject 03, Hour 084

description: Gene expression profile from peripheral blood of subject 03, hour 084


title: Subject 03, Hour 069

description: Gene expression profile from peripheral blood of subject 03, hour 069


title: Subject 03, Hour 077

description: Gene expression profile from peripheral blood of subject 03, hour 077


title: Subject 03, Hour 045

description: Gene expression profile from peripheral blood of subject 03, hour 045


title: Subject 04, Hour 060

description: Gene expression profile from peripheral blood of subject 04, hour 060


title: Subject 03, Hour 060

description: Gene expression profile from peripheral blood of subject 03, hour 060


title: Subject 03, Hour 053

description: Gene expression profile from peripheral blood of subject 03, hour 053


title: Subject 04, Hour 045

description: Gene expression profile from peripheral blood of subject 04, hour 045


title: Subject 03, Hour 036

description: Gene expression profile from peripheral blood of subject 03, hour 036


title: Subject 04, Hour 005

description: Gene expression profile from peripheral blood of subject 04, hour 005

value: Hour 005


title: Subject 03, Hour 021

description: Gene expression profile from peripheral blood of subject 03, hour 021

value: Asymptomatic


title: Subject 03, Hour 012

description: Gene expression profile from peripheral blood of subject 03, hour 012


title: Subject 03, Hour 005

description: Gene expression profile from peripheral blood of subject 03, hour 005


title: Subject 03, Hour 029

description: Gene expression profile from peripheral blood of subject 03, hour 029

value: Hour 029


title: Subject 03, Baseline

description: Gene expression profile from peripheral blood of subject 03, baseline


title: Subject 03, Hour 00

description: Gene expression profile from peripheral blood of subject 03, hour 00

source_name: peripheral blood of subject 03, hour 00


title: Subject 02, Hour 101

description: Gene expression profile from peripheral blood of subject 02, hour 101

value: Hour 101


title: Subject 02, Hour 108

description: Gene expression profile from peripheral blood of subject 02, hour 108


title: Subject 02, Hour 093

description: Gene expression profile from peripheral blood of subject 02, hour 093

value: Hour 093


title: Subject 02, Hour 084

description: Gene expression profile from peripheral blood of subject 02, hour 084


title: Subject 02, Hour 077

description: Gene expression profile from peripheral blood of subject 02, hour 077

value: Hour 077


title: Subject 02, Hour 021

description: Gene expression profile from peripheral blood of subject 02, hour 021


title: Subject 02, Hour 029

description: Gene expression profile from peripheral blood of subject 02, hour 029


title: Subject 02, Hour 069

description: Gene expression profile from peripheral blood of subject 02, hour 069

value: Hour 069


title: Subject 02, Hour 060

description: Gene expression profile from peripheral blood of subject 02, hour 060

value: Hour 060


title: Subject 02, Hour 005

description: Gene expression profile from peripheral blood of subject 02, hour 005


title: Subject 02, Hour 045

description: Gene expression profile from peripheral blood of subject 02, hour 045

value: Hour 045


title: Subject 02, Hour 036

description: Gene expression profile from peripheral blood of subject 02, hour 036

value: Hour 036


title: Subject 02, Baseline

description: Gene expression profile from peripheral blood of subject 02, baseline


title: Subject 01, Hour 093

description: Gene expression profile from peripheral blood of subject 01, hour 093


title: Subject 02, Hour 00

description: Gene expression profile from peripheral blood of subject 02, hour 00

source_name: peripheral blood of subject 02, hour 00


title: Subject 01, Hour 060

description: Gene expression profile from peripheral blood of subject 01, hour 060


title: Subject 01, Hour 108

description: Gene expression profile from peripheral blood of subject 01, hour 108


title: Subject 01, Hour 084

description: Gene expression profile from peripheral blood of subject 01, hour 084

value: Hour 084


title: Subject 01, Hour 036

description: Gene expression profile from peripheral blood of subject 01, hour 036


title: Subject 01, Hour 029

description: Gene expression profile from peripheral blood of subject 01, hour 029


title: Subject 01, Hour 045

description: Gene expression profile from peripheral blood of subject 01, hour 045


title: Subject 01, Hour 077

description: Gene expression profile from peripheral blood of subject 01, hour 077


title: Subject 01, Hour 053

description: Gene expression profile from peripheral blood of subject 01, hour 053


title: Subject 01, Hour 069

description: Gene expression profile from peripheral blood of subject 01, hour 069


title: Subject 01, Hour 012

description: Gene expression profile from peripheral blood of subject 01, hour 012

value: Hour 012


title: Subject 01, Hour 005

description: Gene expression profile from peripheral blood of subject 01, hour 005

value: peripheral blood


title: Subject 01, Hour 101

description: Gene expression profile from peripheral blood of subject 01, hour 101


title: Subject 01, Hour 021

description: Gene expression profile from peripheral blood of subject 01, hour 021

value: Hour 021


title: Subject 01, Hour 00

description: Gene expression profile from peripheral blood of subject 01, hour 00

source_name: peripheral blood of subject 01, hour 00


title: Subject 02, Hour 053

description: Gene expression profile from peripheral blood of subject 02, hour 053

value: Hour 053


title: Subject 01, Baseline

description: Gene expression profile from peripheral blood of subject 01, baseline

value: Baseline

value: Symptomatic


title: GN.BM#2

status: Public on Oct 08 2010

description: GN.BM#2, PMN from BM

source_name: femur

extract_protocol: Flushed BM with syringe

value: 6 weeks

value: CD11b+ Ly6-G+

value: dissection/trimming

value: male


title: GN.BM#1

description: GN.BM#1, PMN from BM


title: GN.BM#3

description: GN.BM#3, PMN from BM


title: GN.BM#4

description: GN.BM#4, PMN from BM


title: DF Patient 8

status: Public on Sep 01 2010

city: Pittsburgh

first: Ernesto

last: Marques

state: PA

address: 9022 BST3 3501 Fifth Avenue

department: Infectious Diseases and Microbiology

institute: University of Pittsburgh

description: Gene Expression Data from PBMCs

hyb_protocol: Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on U133 Plus 2.0 DNA Chip . GeneChips were washed and stained in the Affymetrix Fluidics Station 450.

scan_protocol: Genechips were scanned with high resolution Affymetrix GeneChip Scanner 3,000 with autoloader

data_processing: Patient data quality-control, statistical analysis, and plotting were carried out using Affymetrix MAS 5.0 software and the open source R statistical package, version 2.5.0 and add-on libraries, in particular the BioConductor library, version 1.16

source_name: PBMCs from DF patient

label_protocol: 10-100ug of total RNA was used for cRNA synthesis through two-cycle target labeling kit (Affymetrix) according to the manual manufacturer.

growth_protocol: Blood samples from dengue patients (DF and DHF) as well as ND patients enrolled in this study were collected in heparin vacutainer tubes (BD Vacutainer) and within 2 hours from the collection, PBMC samples were separated by gradient density using Ficoll-Paque (Amersham Biosciences)

extract_protocol: Cells were lysed with Trizol (Invitrogen) for total RNA extraction through chloroform extraction and isopropyl alcohol precipitation following manufacturer’s protocol

treatment_protocol: 4 millions PBMC cryopreserved in 10% (v/v) Dimethyl sulfoxide (DMSO; Sigma-Aldrich) in inactivated fetal bovine sera (FBS; Hyclone) were used for RNA extraction

tag: gender

value: female

tag: days of symptoms

tag: igm

value: Pos

tag: igg

value: Neg

tag: pcr/virus isolation


title: DF Patient 5

description: Gene Expression Data from PBMCs


title: DHF Patient 7

description: Gene Expression Data from PBMCs

source_name: PBMCs from DHF patient


title: DHF Patient 5

description: Gene Expression Data from PBMCs


title: ND Patient 7

description: Gene Expression Data from PBMCs

source_name: PBMCs from ND patient


title: ND Patient 2

description: Gene Expression Data from PBMCs


title: DHF Patient 1

description: Gene Expression Data from PBMCs


title: DHF Patient 3

description: Gene Expression Data from PBMCs


title: ND Patient 8

description: Gene Expression Data from PBMCs


title: DHF Patient 9

description: Gene Expression Data from PBMCs


title: DF Patient 6

description: Gene Expression Data from PBMCs


title: DHF Patient 6

description: Gene Expression Data from PBMCs


title: DF Patient 4

description: Gene Expression Data from PBMCs


title: ND Patient 1

description: Gene Expression Data from PBMCs


title: DHF Patient 10

description: Gene Expression Data from PBMCs


title: ND Patient 4

description: Gene Expression Data from PBMCs


title: DHF Patient 8

description: Gene Expression Data from PBMCs


title: DHF Patient 4

description: Gene Expression Data from PBMCs


title: DF Patient 2

description: Gene Expression Data from PBMCs


title: ND Patient 3

description: Gene Expression Data from PBMCs


title: DF Patient 7

description: Gene Expression Data from PBMCs


title: ND Patient 6

description: Gene Expression Data from PBMCs


title: DHF Patient 2

description: Gene Expression Data from PBMCs


title: Subject 02, Hour 012

description: Gene expression profile from peripheral blood of subject 02, hour 012

value: Asymptomatic


title: DF Patient 1

description: Gene Expression Data from PBMCs


title: DF Patient 3

description: Gene Expression Data from PBMCs


title: ND Patient 5

description: Gene Expression Data from PBMCs


title: Subject 09, Hour 005

description: Gene expression profile from peripheral blood of subject 09, hour 005

value: Hour 005


title: Influenza A H3N2 challenge sample at baseline_Flu015

status: Public on Aug 06 2009

city: Durham

first: Geoffrey

middle: S

last: Ginsburg

state: NC

address: 470 Research Drive, Room 254, box 90141

department: Center for Genomic Medicine

institute: Duke Institute for Genome Sciences & Policy

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.symptomatic_baseline15

hyb_protocol: 20 ug of antisense cRNA was hybridized for 16+ hr at 45C on GeneChip Human U133A array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Midi_euk2v3_450 protocol

scan_protocol: GeneChips were scanned using the Affymetrix GeneArray Scanner 3000 7G

data_processing:
analysis - Retrospectively, infected versus noninfected individuals were determined by respiratory viral symptom score and confirmed by viral culture and/or PCR of nasal washes. Time of peak symptoms was selected for analysis.  Attack rate was approximately 50% for each challenge, meaning half of the subjects in each separate challenge (influenza, RSV or rhinovirus) developed symptoms of  infection.
The data were analyzed with GeneChip Operating System (GCOS) using Affymetrix default analysis settings and global scaling to a target intensity of 500 as normalization method.

source_name: RNA from Peripheral human blood from adult participating in influenza A N3N2 challenge study

label_protocol: Biotinylated antisense strand cRNA was prepared according to the standard Affymetrix protocol from 2.5 ug total RNA utilizing peptide nucleic acids complimentary to globin mRNA to block amplification of those transcripts(GeneChip® 1 cycle labeling kit with Globin Reduction protocol (Affymetrix) Data was Generated at Expression Analysis, Inc (Durham, NC))

extract_protocol:
inoculation protocol - Healthy adult volunteers were inoculated intranasally with either influenza H3N2, rhinovirus or respiratory syncytial virus.  Symptoms were recorded at standard intervals and a time course of peripheral blood was collected in RNA PaxGene tubes.
Total RNA extracted from PaxGene Blood tubes as per the manufacturer's protocol.

tag: subject id

tag: timepoint

value: baseline

tag: symptom group


title: Influenza A H3N2 challenge sample at T_Flu017

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.asymptomatic_peak17

value: T - peak symptoms


title: Influenza A H3N2 challenge sample at T_Flu014

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.asymptomatic_peak14


title: Influenza A H3N2 challenge sample at baseline_Flu017

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.asymptomatic_baseline17


title: Influenza A H3N2 challenge sample at T_Flu016

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.asymptomatic_peak16


title: Influenza A H3N2 challenge sample at T_Flu012

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.symptomatic_peak12


title: Influenza A H3N2 challenge sample at T_Flu015

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.symptomatic_peak15


title: Influenza A H3N2 challenge sample at T_Flu010

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.symptomatic_peak10


title: Influenza A H3N2 challenge sample at baseline_Flu014

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.asymptomatic_baseline14


title: Influenza A H3N2 challenge sample at baseline_Flu012

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.symptomatic_baseline12


title: Influenza A H3N2 challenge sample at T_Flu007

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.symptomatic_peak07


title: Influenza A H3N2 challenge sample at baseline_Flu013

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.symptomatic_baseline13


title: Influenza A H3N2 challenge sample at baseline_Flu007

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.symptomatic_baseline07


title: Influenza A H3N2 challenge sample at T_Flu008

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.symptomatic_peak08


title: Influenza A H3N2 challenge sample at T_Flu013

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.symptomatic_peak13


title: Influenza A H3N2 challenge sample at T_Flu011

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.asymptomatic_peak11


title: Influenza A H3N2 challenge sample at T_Flu009

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.asymptomatic_peak09


title: Influenza A H3N2 challenge sample at baseline_Flu011

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.asymptomatic_baseline11


title: Influenza A H3N2 challenge sample at baseline_Flu016

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.asymptomatic_baseline16


title: Influenza A H3N2 challenge sample at T_Flu004

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.asymptomatic_peak04


title: Influenza A H3N2 challenge sample at baseline_Flu009

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.asymptomatic_baseline09


title: Influenza A H3N2 challenge sample at baseline_Flu005

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.symptomatic_baseline05


title: Flow sorted normal MEP cells, 2

status: Public on Feb 21 2010

city: Lund

first: Thoas

middle: Anna

address: Lund University Hospital

country: Sweden

institute: Dept of Clinical Genetics

hyb_protocol: As per manufacturer (Affymetrix)

scan_protocol: As per manufacturer (Affymetrix)

data_processing: Data were analyzed using Bioconductor and normalization was performed using gcRMA within Biocinductor

source_name: Normal MEP cells

label_protocol: RNA was amplified using the MessageAmp™ II-Biotin Enhanced Single Round aRNA Amplification (Ambion)

extract_protocol: Flow sorted cells from adult bone marrow. Trizol extraction of total RNA was performed according to the manufacturer's instructions.

value: normal flow sorted bone marrow

tag: cell type

value: Normal MEP cells


title: Influenza A H3N2 challenge sample at baseline_Flu010

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.symptomatic_baseline10


title: Influenza A H3N2 challenge sample at baseline_Flu008

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.symptomatic_baseline08


title: Influenza A H3N2 challenge sample at T_Flu006

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.symptomatic_peak06


title: Flow sorted normal MEP cells, 1


title: Influenza A H3N2 challenge sample at baseline_Flu006

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.symptomatic_baseline06


title: Influenza A H3N2 challenge sample at T_Flu005

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.symptomatic_peak05


title: Influenza A H3N2 challenge sample at T_Flu002

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.asymptomatic_peak02


title: Influenza A H3N2 challenge sample at baseline_Flu003

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.asymptomatic_baseline03


title: Influenza A H3N2 challenge sample at baseline_Flu004

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.asymptomatic_baseline04


title: Influenza A H3N2 challenge sample at baseline_Flu001

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.symptomatic_baseline01


title: Influenza A H3N2 challenge sample at baseline_Flu002

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.asymptomatic_baseline02


title: respiratory syncytial virus challenge sample at T_RSV015

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.asymptomatic_peak15

source_name: RNA from peripheral human blood from adult participating in respiratory syncytial virus challenge study


title: Influenza A H3N2 challenge sample at T_Flu003

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.asymptomatic_peak03


title: respiratory syncytial virus challenge sample at T_RSV018

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.asymptomatic_peak18


title: respiratory syncytial virus challenge sample at T_RSV012

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.symptomatic_peak12


title: respiratory syncytial virus challenge sample at baseline_RSV020

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.symptomatic_baseline20


title: respiratory syncytial virus challenge sample at baseline_RSV018

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.asymptomatic_baseline18


title: Influenza A H3N2 challenge sample at T_Flu001

description:
Gene expression data from adult human volunteer challenged with influenza A H3N2.
flu.symptomatic_peak01


title: respiratory syncytial virus challenge sample at baseline_RSV015

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.asymptomatic_baseline15


title: respiratory syncytial virus challenge sample at T_RSV013

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.asymptomatic_peak13


title: respiratory syncytial virus challenge sample at baseline_RSV017

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.asymptomatic_baseline17


title: respiratory syncytial virus challenge sample at baseline_RSV012

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.symptomatic_baseline12


title: respiratory syncytial virus challenge sample at baseline_RSV013

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.asymptomatic_baseline13


title: respiratory syncytial virus challenge sample at T_RSV017

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.asymptomatic_peak17


title: respiratory syncytial virus challenge sample at baseline_RSV014

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.symptomatic_baseline14


title: respiratory syncytial virus challenge sample at baseline_RSV016

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.asymptomatic_baseline16


title: respiratory syncytial virus challenge sample at T_RSV019

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.asymptomatic_peak19


title: respiratory syncytial virus challenge sample at T_RSV011

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.symptomatic_peak11


title: respiratory syncytial virus challenge sample at T_RSV020

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.symptomatic_peak20


title: respiratory syncytial virus challenge sample at baseline_RSV019

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.asymptomatic_baseline19


title: respiratory syncytial virus challenge sample at baseline_RSV011

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.symptomatic_baseline11


title: respiratory syncytial virus challenge sample at baseline_RSV010

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.asymptomatic_baseline10


title: respiratory syncytial virus challenge sample at T_RSV010

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.asymptomatic_peak10


title: respiratory syncytial virus challenge sample at T_RSV009

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.asymptomatic_peak09


title: respiratory syncytial virus challenge sample at T_RSV014

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.symptomatic_peak14


title: respiratory syncytial virus challenge sample at baseline_RSV009

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.asymptomatic_baseline09


title: respiratory syncytial virus challenge sample at T_RSV016

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.asymptomatic_peak16


title: respiratory syncytial virus challenge sample at T_RSV008

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.asymptomatic_peak08


title: respiratory syncytial virus challenge sample at baseline_RSV008

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.asymptomatic_baseline08


title: respiratory syncytial virus challenge sample at T_RSV005

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.asymptomatic_peak05


title: respiratory syncytial virus challenge sample at baseline_RSV007

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.symptomatic_baseline07


title: respiratory syncytial virus challenge sample at T_RSV004

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.asymptomatic_peak04


title: respiratory syncytial virus challenge sample at baseline_RSV005

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.asymptomatic_baseline05


title: respiratory syncytial virus challenge sample at baseline_RSV006

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.symptomatic_baseline06


title: respiratory syncytial virus challenge sample at baseline_RSV004

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.asymptomatic_baseline04


title: respiratory syncytial virus challenge sample at T_RSV006

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.symptomatic_peak06


title: respiratory syncytial virus challenge sample at baseline_RSV003

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.symptomatic_baseline03


title: rhinovirus challenge sample at T_Rhino020

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.symptomatic_peak20

source_name: RNA from peripheral human blood from adult participating in rhinovirus challenge study


title: respiratory syncytial virus challenge sample at T_RSV003

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.symptomatic_peak03


title: rhinovirus challenge sample at T_Rhino019

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.symptomatic_peak19


title: respiratory syncytial virus challenge sample at T_RSV001

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.symptomatic_peak01


title: rhinovirus challenge sample at baseline_Rhino019

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.symptomatic_baseline19


title: respiratory syncytial virus challenge sample at T_RSV002

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.symptomatic_peak02


title: rhinovirus challenge sample at baseline_Rhino018

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.asymptomatic_baseline18


title: respiratory syncytial virus challenge sample at baseline_RSV001

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.symptomatic_baseline01


title: respiratory syncytial virus challenge sample at baseline_RSV002

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.symptomatic_baseline02


title: rhinovirus challenge sample at T_Rhino018

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.asymptomatic_peak18


title: rhinovirus challenge sample at baseline_Rhino015

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.symptomatic_baseline15


title: rhinovirus challenge sample at baseline_Rhino020

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.symptomatic_baseline20


title: respiratory syncytial virus challenge sample at T_RSV007

description:
Gene expression data from adult human volunteer challenged with respiratory syncytial virus (RSV).
rsv.symptomatic_peak07


title: rhinovirus challenge sample at T_Rhino017

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.asymptomatic_peak17


title: rhinovirus challenge sample at T_Rhino014

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.asymptomatic_peak14


title: rhinovirus challenge sample at T_Rhino012

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.asymptomatic_peak12


title: rhinovirus challenge sample at T_Rhino015

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.symptomatic_peak15


title: rhinovirus challenge sample at T_Rhino016

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.symptomatic_peak16


title: rhinovirus challenge sample at baseline_Rhino014

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.asymptomatic_baseline14


title: rhinovirus challenge sample at baseline_Rhino011

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.symptomatic_baseline11


title: rhinovirus challenge sample at T_Rhino013

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.asymptomatic_peak13


title: rhinovirus challenge sample at baseline_Rhino013

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.asymptomatic_baseline13


title: rhinovirus challenge sample at baseline_Rhino017

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.asymptomatic_baseline17


title: rhinovirus challenge sample at baseline_Rhino010

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.asymptomatic_baseline10


title: rhinovirus challenge sample at T_Rhino009

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.symptomatic_peak09


title: rhinovirus challenge sample at baseline_Rhino008

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.asymptomatic_baseline08


title: rhinovirus challenge sample at T_Rhino007

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.symptomatic_peak07


title: rhinovirus challenge sample at baseline_Rhino009

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.symptomatic_baseline09


title: rhinovirus challenge sample at baseline_Rhino012

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.asymptomatic_baseline12


title: rhinovirus challenge sample at T_Rhino010

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.asymptomatic_peak10


title: rhinovirus challenge sample at baseline_Rhino005

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.asymptomatic_baseline05


title: rhinovirus challenge sample at T_Rhino008

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.asymptomatic_peak08


title: rhinovirus challenge sample at T_Rhino005

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.asymptomatic_peak05


title: rhinovirus challenge sample at T_Rhino002

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.asymptomatic_peak02


title: rhinovirus challenge sample at T_Rhino006

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.symptomatic_peak06


title: rhinovirus challenge sample at T_Rhino004

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.symptomatic_peak04


title: rhinovirus challenge sample at baseline_Rhino007

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.symptomatic_baseline07


title: rhinovirus challenge sample at T_Rhino011

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.symptomatic_peak11


title: rhinovirus challenge sample at baseline_Rhino006

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.symptomatic_baseline06


title: rhinovirus challenge sample at baseline_Rhino003

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.symptomatic_baseline03


title: rhinovirus challenge sample at baseline_Rhino002

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.asymptomatic_baseline02


title: rhinovirus challenge sample at baseline_Rhino001

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.asymptomatic_baseline01


title: rhinovirus challenge sample at T_Rhino001

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.asymptomatic_peak01


title: rhinovirus challenge sample at baseline_Rhino004

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.symptomatic_baseline04


title: PBMC_InfluenzaA_INF536

status: Public on Mar 16 2007

city: Dallas

first: Damien

last: Chaussabel

state: TX

address: 3434 Live Oak

institute: Baylor Institute for Immunology Research

description: The subject was infected with Influenza A.

scan_protocol: GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 System.

data_processing: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.

label_protocol: Biotinylated cRNA were prepared according to the standard Affymetrix protocol.

extract_protocol: RNeasy mini

tag: Age

value: 2y, when sample taken

tag: Gender

value: F

tag: Race

value: Hispanic

tag: Illness

value: Fever

tag: Treatment

tag: Pathogen

value: Influenza A


title: PBMC_S.aureus_MRSA_INF380

description: The subject was infected with S. aureus, MRSA.

source_name: PBMC_S. aureus

value: 7.5y, when sample taken

value: M

value: Black

value: Osteomyelitis/Suppurative Arthritis

value: Clindamycin

value: S. aureus, MRSA


title: rhinovirus challenge sample at T_Rhino003

description:
Gene expression data from adult human volunteer challenged with rhinovirus
rhino.symptomatic_peak03

value: Symptomatic


title: PBMC_S.aureus_MRSA_INF369

value: 14y, when sample taken

value: Disseminated

value: Vancomycin/Rifampin


title: PBMC_InfluenzaA_INF547

value: 1.3y, when sample taken

value: Encephalitis

value: Ceftriaxone/Oseltamivir


title: PBMC_InfluenzaA_INF540

value: 0.08y, when sample taken

value: Ampicillin/Gentamicin


title: Donor 2, fresh monocytes

status: Public on Dec 31 2008

city: New York

first: Janice

last: Chen

state: NY

address: 535 East 70th St, 4th floor Research

department: Research

institute: Hospital for Special Surgery

description: Donor 2, fresh monocytes

hyb_protocol: Samples were hybridized to Affymetrix HG U133 Plus 2.0 GeneChip using Affymetrix fluidics station according to the instructions of the manufacturer.

scan_protocol: Affymetrix Gene-Array Scanner

data_processing: Data was processed using the Affymetrix Microarray Suite. Further data analysis was performed using GeneSpring software.

source_name: Donor 2, fresh monocytes

label_protocol: Labeled cRNA was prepared with the Affymetrix GeneChip IVT Labeling Kit.

extract_protocol: Total RNA was extracted using TRIzol Reagent (Invitrogen), followed by clean-up with the RNeasy Mini Kit (Qiagen).

treatment_protocol: No treatment; RNA was extracted from cells immediately upon isolation of CD14+ human monocytes from peripheral blood.

tag: Peripheral blood from healthy human donor


title: PBMC_S.aureus_MRSA_INF374

value: 1.75y, when sample taken

value: Bacteremia/Suppurative arthritis

value: Vancomycin


title: PBMC_InfluenzaB_INF533

description: The subject was infected with Influenza B.

value: 11y, when sample taken

value: M

value: Caucasian

value: Fever

value: Influenza B


title: PBMC_InfluenzaA_INF517

value: 0.5y, when sample taken

value: F

value: Hispanic

value: Pneumonia

value: Influenza A


title: PBMC_InfluenzaB_INF549

value: 13y, when sample taken

value: Ceftriaxone/Vancomycin/Oseltamivir


title: PBMC_S.aureus_MSSA_INF308

description: The subject was infected with S. aureus, MSSA.

value: 12y, when sample taken

value: Black

value: Disseminated/Pneumonia

value: Oxacillin/Clindamycin

value: S. aureus, MSSA


title: PBMC_S.aureus_MRSA_INF372

value: 14y, when sample taken

value: Osteomyelitis/Bacteremia

value: Vancomycin/Rifampin

value: S. aureus, MRSA


title: PBMC_InfluenzaA_INF532

value: 0.1y, when sample taken

value: Fever

value: Ampicillin/Gentamicin


title: PBMC_S.aureus_MSSA_INF305

value: 4.5y, when sample taken

value: F

value: Hispanic

value: Cefazolin


title: PBMC_S.pneumoniae_INF287_j2141124

description: The subject was infected with S. pneumoniae.

source_name: PBMC_S. pneumoniae

value: 3y, when sample taken

value: Caucasian

value: Pneumonia/Bacteremia

value: Ceftriaxone/Vancomycin

value: S. pneumoniae


title: PBMC_S.pneumoniae_INF265_j6492221

value: 2 y, when sample taken

value: Pneumonia/Empyema


title: Donor 1, fresh monocytes

description: Donor 1, fresh monocytes

source_name: Donor 1, fresh monocytes

growth_protocol: RNA was extracted from cells immediately upon isolation of CD14+ human monocytes from peripheral blood.


title: PBMC_InfluenzaA_INF524

value: 6y, when sample taken

value: M

value: Influenza A


title: PBMC_S.pneumoniae_INF277_j0627842

value: 16y, when sample taken


title: PBMC_S.pneumoniae_INF096_j5808206

value: 16 m, when sample taken

value: Ceftriaxone/Azithromycin


title: PBMC_S.pneumoniae_INF289_j5197307


title: PBMC_InfluenzaB_INF567

description: The subject was infected with Influenza B .

value: 2y, when sample taken

value: Fever/URI

value: Influenza B


title: PBMC_InfluenzaA_INF553

value: 18m, when sample taken

value: F

value: Oseltamivir


title: PBMC_S.pneumoniae_INF268_j2754553

value: 3y, when sample taken

value: Hispanic

value: Ceftriaxone/Clindamycin