title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_D1_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_D12_ad010

status: Public on Dec 01 2020

type: SRA

city: La Jolla

first: Joseph

middle: R

last: Ecker

state: CA

address: 10010 North Torrey Pines Road

department: Genomic Analysis Laboratory

country: USA

institute: HHMI-Salk-Institute

library_source: genomic

data_processing:
Reads trimed with Cutadapt for adapters and low quality bases (Phred score >= 20)
The resulting files are mapped to in-silico bisulfite converted mm10 INTACT reference genome using Bismark.
The raw BAM file will be filtered using samtools to filter reads by MAPQ > 10 and use Picard to remove PCR duplicates
Tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls will be generated with in-house developing package using the filtered BAM files
Genome_build: mm10 INTACT
Supplementary_files_format_and_content: tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls. Columns are: 1. Chromosome, 2. Position, 3. Strand, 4. Cytosin Context, 5. methylated base call, 6. total mapped base call, 7. not used, place holder column

molecule: genomic DNA

organism: Mus musculus

source_name: C57BL/6J P56 Mouse Brain

growth_protocol: All experimental procedures using live animals were approved by the Salk Institute Animal Care and Use Committee. The knock-in mouse line, R26R-CAG-loxp-stop-loxp-Sun1-sfGFP-Myc (INTACT) was used for most experiments4 and they were maintained on a C57BL/6J background. 42-49 day old adult male and female INTACT mice were used for the retrograde labeling experiment.

extract_protocol:
Approximately two weeks after the AAVretro injection, brains were extracted from the 56-63 day old INTACT mice, immediately submerged in ice-cold slicing buffer (2.5mM KCl, 0.5mM CaCl2, 7mM MgCl2, 1.25mM NaH2PO4, 110mM sucrose, 10mM glucose and 25mM NaHCO3) that was bubbled with carbogen, and sliced into 0.6 mm coronal sections starting from the frontal pole. From each AAVretro-injected brain, the slices were kept in the ice-cold dissection buffer from which selected brain regions (Supplementary Table 1) were manually dissected under a fluorescent dissecting microscope (Olympus SZX16), following the Allen Mouse Common Coordinate Framework (CCF), Reference Atlas, Version 3 (2015) (Extended Data Fig. 1). The dissected brain tissues were transferred to prelabeled microcentrifuge tubes, immediately frozen in dry ice, and subsequently stored at -80oC.   For each dissected brain region, samples from 2 males and 2 females were pooled separately as biological replicates for nuclei preparation. The 2-mL glass tissue dounce homogenizer and pestles (Sigma-Aldrich D8938-1SET) were pre-chilled on ice. Nuclei were prepared using a modified protocol as reported by Lacar et al., 201622. In summary, the frozen brain tissues were transferred to the dounce homogenizer with 1 mL ice-cold NIM buffer (0.25M sucrose, 25mM KCl, 5mM MgCl2, 10mM Tris-HCl (pH7.4), 1mM DTT (Sigma 646563), 10μl of protease inhibitor (Sigma P8340)), with 0.1% Triton X-100 and 5μM Hoechst 33342 (Invitrogen H3570), and gently homogenized on ice with the pestle 10-15 times. The homogenate was transferred to pre-chilled microcentrifuge tubes and centrifuged at 1000 rcf for 8 min at 4oC to pellet the nuclei. The pellet was resuspended in 1 mL ice-cold NIM buffer, and again centrifuged at 1000 rcf for 8 min at 4oC. The pellet was then resuspended in 450 μL of ice-cold NSB buffer (0.25M sucrose, 5mM MgCl2, 10mM Tris-HCl (pH7.4), 1mM DTT, 9ul of Protease inhibitor), and filtered through 40μM cell strainer. The filtered nuclei suspension was incubated on ice for at least 30 minutes with 50μl of nuclease-free BSA for at least 10 minutes, then incubated with GFP antibody, Alexa Fluor 488 (Invitrogen, A-21311) and anti-NeuN antibody (EMD Millipore MAB377) conjugated with Alexa Fluor 647 (Invitrogen A20173). GFP+/NeuN+ single nuclei were isolated using fluorescence-activated nuclei sorting (FANS) on a BD Influx sorter with 100μm nozzle, and sorted into 384-well plates preloaded with 2μl of digestion buffer for snmC-seq215 (20 mL digestion buffer consists of 10 mL M-digestion buffer (2×, Zymo D5021-9), 1 ml Proteinase K (20 mg, Zymo D3001-2-20), 9 mL water, and 10 µL unmethylated lambda DNA (100 pg/µL, Promega, D1521)). The collected plates were incubated at 50oC for 20 minutes then stored at -20 oC.
The bisulfite conversion and library preparation were performed following the detailed snmC-seq2 protocol as previously described (Luo, C. et al., Nat. Commun. 2018, 9(1):3824.). The snmC-Seq2 libraries were sequenced on Illumina Novaseq 6000 using the S4 flow cell 2 x 150 bp mode.

treatment_protocol: To label neurons projecting to regions of interest, injections of rAAV2-retro-Cre (produced by Salk Vector Core or Vigene, 2x10^12 to 1x10^13 viral genomes/ml, produced with capsid from Addgene plasmid #81070 packaging pAAV-EF1a-Cre from Addgene plasmid #55636) were made into both hemispheres of the INTACT mice. Animals were anesthetized with either ketamine/xylazine or isoflurane, placed in a stereotaxic frame, and 0.1 to 0.5 microliters of AAV was injected by pressure into stereotaxic coordinates corresponding to the desired projection target.

tag: strain

tag: age

tag: tissue

value: brain

tag: source

value: AI

tag: target

value: VISp

tag: gender

value: female


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_D12_ad007

value: male


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_D12_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_D12_ad004

value: AUD


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_D3_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_D12_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_D1_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_D11_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_D11_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_D11_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_D11_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_D11_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_D10_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_D11_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_D10_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_C9_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_D1_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_C9_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_C9_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_D10_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_D10_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_D10_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_D10_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_C8_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_C9_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_C8_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_C8_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_C8_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_C8_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_C8_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_D12_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_D11_ad012


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title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_C8_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_C7_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_C7_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_C7_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_C6_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_C7_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_C6_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_C9_ad006


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title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_C5_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_C5_ad007


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title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_C5_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_C4_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_C4_ad004


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title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_C3_ad007


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title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_C2_ad010


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title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_C1_ad007


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title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B9_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_C10_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_C10_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_C10_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B9_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_C10_ad001


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title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B9_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B9_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B8_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_C12_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B8_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B8_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B8_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B8_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B8_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B7_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B7_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B8_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B7_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B7_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B7_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_C10_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B7_ad001


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title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B6_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B6_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B9_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B5_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B6_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B5_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B5_ad007


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title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B4_ad008


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title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B4_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B7_ad010


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title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B3_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B3_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B3_ad002


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title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B3_ad001


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title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B2_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B2_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B5_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B2_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B1_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B1_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B1_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B1_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B1_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B4_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B1_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B1_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B12_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B12_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B12_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B12_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B12_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B12_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B12_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B11_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B11_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B2_ad004


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title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B11_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B11_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B2_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B10_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B10_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B10_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B10_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B10_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A8_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A9_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A8_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A9_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A8_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A8_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A8_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B10_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A7_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A7_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A7_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A7_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A7_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A7_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A6_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_B10_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A6_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A6_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A6_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A5_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A6_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A5_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A9_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A5_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A5_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A5_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A4_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A5_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A4_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A4_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A4_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A9_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A3_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A3_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A3_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A3_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A3_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A2_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A2_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A2_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A2_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A2_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A2_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A2_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A1_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A1_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A1_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A1_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A1_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A1_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A12_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A12_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A12_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A12_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A3_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A12_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A3_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A12_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A11_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A11_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A11_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A11_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A12_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A11_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A3_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A11_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A10_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A10_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A10_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A10_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A11_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H9_ad006

value: SSp

value: VTA


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A10_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H8_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H8_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H8_ad008

value: ACA


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H8_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H9_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H8_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H7_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H7_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H6_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H6_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H6_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H6_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H7_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H5_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P5_190607_P6_A10_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H5_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H5_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H5_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H4_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H4_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H5_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H4_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H4_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H4_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H3_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H3_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H2_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H9_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H3_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H2_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H2_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H1_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H6_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H1_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H1_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H12_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H1_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H12_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H2_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H12_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H12_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H12_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H12_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H11_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H11_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H11_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H10_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H10_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H10_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H10_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H10_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G9_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G9_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G9_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G9_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H2_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H11_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G9_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G9_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G8_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G6_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G7_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G8_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G7_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G7_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G6_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G6_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G8_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G8_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G6_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G7_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_H10_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G6_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G6_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G5_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G5_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G5_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G4_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G4_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G5_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G4_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G4_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G3_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G3_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G8_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G3_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G3_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G3_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G3_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G2_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G2_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G6_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G2_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G12_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G1_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G12_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G12_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G11_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G4_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G11_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G11_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G11_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G11_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G10_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G10_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_F9_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G12_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_F8_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_F8_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G1_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_F9_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_F8_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_F9_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_F9_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G1_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_F7_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_F7_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G1_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_F7_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_F7_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_F6_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_F6_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_F6_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_G10_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_F6_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_F5_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P3_190607_P4_F5_ad012


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value: MOp


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title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_B11_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_B10_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_B10_ad004


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_B10_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A9_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_B10_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_B10_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A9_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A9_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A8_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_B11_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A6_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A7_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A5_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A6_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A5_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A5_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A3_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A4_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A3_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A3_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A2_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A1_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A1_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A12_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A12_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A2_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A12_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A8_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A8_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A8_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A11_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A11_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A11_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A4_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A11_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A10_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A10_ad008


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A11_ad002


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_C8_ad010


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A10_ad007


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A11_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A10_ad012


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A10_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A2_ad008


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H9_ad004

value: MY


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_A12_ad007


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H9_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H9_ad002


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H8_ad006


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_D11_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H8_ad002


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H6_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H7_ad008


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H5_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H7_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H5_ad008


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H5_ad007


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H5_ad006


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H5_ad001


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H4_ad008


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H4_ad002


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H3_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H4_ad001


title: 190607_CEMBA_mm_P56_P63_RS2_190607_P1_190607_P2_D11_ad008


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H3_ad008


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H3_ad007


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H2_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H2_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H3_ad001


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H8_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H1_ad007


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H2_ad007


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H1_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H7_ad006


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H1_ad002


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H12_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H12_ad006


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H12_ad004


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H12_ad002


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H11_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H11_ad007


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H11_ad008


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H10_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H11_ad004


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H10_ad006


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H10_ad007


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G9_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G9_ad007


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_H10_ad008


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G9_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G9_ad004


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G8_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G8_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G8_ad008


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G8_ad007


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G8_ad006


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G5_ad008


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G5_ad002


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G5_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G5_ad007


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G8_ad004


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G8_ad001


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G7_ad007


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G7_ad006


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G7_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G8_ad002


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G4_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G4_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G3_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G5_ad001


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G6_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G6_ad002


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G3_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G2_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G2_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G2_ad008


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G2_ad007


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G2_ad006


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G1_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G1_ad007


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G6_ad008


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G7_ad004


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G12_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G3_ad006


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G4_ad008


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G12_ad007


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_F9_ad001


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G10_ad001


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G12_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G10_ad007


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G10_ad006


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G10_ad008


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G11_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_G11_ad006


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_F9_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_F8_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_F6_ad008


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title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_F7_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_F7_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_F8_ad006


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_F8_ad002


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_F6_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_F5_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_F5_ad007


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_F4_ad006


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_F4_ad010


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title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_F4_ad001


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title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_F3_ad008


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_F3_ad012


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title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_F3_ad002


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title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_F12_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_F1_ad007


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title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_F12_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_F4_ad012


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title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E9_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_F10_ad004


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_F2_ad008


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_F11_ad008


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_F1_ad008


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_F10_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_F1_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E9_ad008


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title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E9_ad004


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E9_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E8_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E9_ad006


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title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E8_ad001


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title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E7_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E6_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E7_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E6_ad002


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E6_ad001


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title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E5_ad002


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title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E4_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E3_ad007


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E2_ad007


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E3_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E3_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E2_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E11_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E12_ad004


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E4_ad002


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E11_ad004


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E1_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E2_ad001


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D9_ad002


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E2_ad006


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D9_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E1_ad001


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E1_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E12_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E12_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E11_ad006


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E11_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E12_ad007


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D8_ad007


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E4_ad008


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D8_ad006


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E4_ad006


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E11_ad001


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D9_ad001


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D8_ad008


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D8_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D6_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D7_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D8_ad002


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D7_ad007


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D7_ad001


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D3_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D9_ad008


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D4_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D3_ad008


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D5_ad001


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D3_ad007


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D3_ad006


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D7_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D5_ad008


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D6_ad008


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D4_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D5_ad007


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D2_ad006


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D2_ad008


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D4_ad002


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D2_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D2_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D2_ad002


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D1_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D5_ad006


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D1_ad002


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D1_ad006


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D7_ad006


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D12_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D12_ad001


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D11_ad004


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D11_ad006


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D12_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D1_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D10_ad002


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D10_ad004


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C8_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D10_ad001


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C8_ad002


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C8_ad004


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C7_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C8_ad001


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C7_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C7_ad007


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C6_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C6_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C7_ad004


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C6_ad007


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C6_ad002


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C5_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_D10_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C4_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C4_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C4_ad007


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C8_ad007


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C5_ad001


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C3_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C4_ad001


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C3_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C2_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C3_ad001


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C2_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C2_ad007


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C8_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C5_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C5_ad008


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C1_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C1_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C12_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C11_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C12_ad007


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C12_ad001


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C11_ad012


title: HCT116_WT_RRBS

city: Bethesda

first: Yaqiang

last: Cao

state: Maryland

address: 9000 Rockville Pike

department: System Biology Center

institute: NHLBI

data_processing:
RRBS reads were mapped to human genome hg38 by bismark (v0.19.0) . Only unique mapped reads were kept and CpGs with >= 5 reads mapped were used for further analysis.  Paired-end 2 replicates RNA-seq reads for HeLa wild type and LSH KO cells were mapped to hg38 by STAR (v2.4.0d) and then quantified by Cuffdiff (v2.2.1) . Differentially expressed genes (DEGs) were required p-value<=1e-2 and fold change >=2 as reported by Cuffdiff.
Supplementary_files_format_and_content: The RRBS processed data were the output of bismark tab-delimited text file for each sample. For RNA-seq data the cuffdiff output differential expressed genes  with name of gene_exp.diff were used.
Genome_build: hg38

organism: Homo sapiens

growth_protocol: Human cervical cancer cell line (HeLa) was maintained in DMEM medium (Gibco) containing 10% fetal calf serum (Gemini), 100 U/mL penicillin and 100μg/mL streptomycin (Milipore). Human colon cancer cells (HCT116) was maintained in McCoy’s 5A medium (Gibco) containing 10% fetal calf serum (Gemini), 100 U/mL penicillin and 100μg/mL streptomycin (Milipore). The cultured cells were maintained at 37 °C in a humidified incubator with 5% CO2.

extract_protocol: To prepare genomic DNA for RRBS analysis, cells were resuspended with cell lysis buffer (10mM Tris pH 7.5, 10 mM EDTA, 10 mM NaCl, 0.5% sarcosyl, 0.1 mg/mL RNase (CWBIO)) and incubated at 37°C overnight. Then, protease K was added to a final concentration of 0.2 mg/mL and incubated at 65°C for 24 hr. Genomic DNA was extracted by phenol/chloroform and ethanol precipitated. The DNA were dissolve in ddH2O.Total RNA for RNA-seq analysis was extracted from cells using RNAiso Plus Reagent (Takara) according to the manufacturer’s instructions.

treatment_protocol: No treatment.

tag: cell line

tag: genotype/variation

value: wild type


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C11_ad008


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C10_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C11_ad001


title: HeLa_WT_RRBS

source_name: HeLa

value: HeLa


title: HCT116_LSH_KO_RRBS

value: LSH KO


title: HeLa_LSH_WT_rep2_RNA-seq

library_source: transcriptomic

molecule: polyA RNA


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C10_ad010


title: HeLa_LSH_WT_rep1_RNA-seq


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C10_ad008


title: HeLa_LSH_KO_rep2_RNA-seq


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C12_ad008


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_B9_ad006


title: HeLa_LSH_KO_rep1_RNA-seq


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_C10_ad007


title: shLAMC2

city: London

first: Matthew

middle: Peter

last: Caley

state: london

address: Centre for Cutaneous Research, BLizard Building, Newark Street

country: United Kingdom

institute: QMUL

data_processing:
FastQC
Tophat2
Cufflinks
CuffDuff
Genome_build: HG19
Supplementary_files_format_and_content: Tab Delimited file including FPKMs for each sample

molecule: total RNA

extract_protocol: RNA libraries were prepared for sequencing using standard Illumina protocols

value: cSCC cell line SCCIC1

tag: treatment

tag: genotype

value: LAMC2 knockdown


title: shLAMA3

value: LAMA3 knockdown


title: shLAMB3

value: LAMB3 knockdown


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_B8_ad006

value: brain


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_B9_ad004


title: shC

value: control


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_B7_ad010

value: female


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_B7_ad008


title: HeLa_LSH_KO_RRBS


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_B7_ad006


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_B7_ad007


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_B7_ad004

value: male


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_B6_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_B7_ad002


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_B8_ad007


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_B6_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_E8_ad004


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_B5_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_B8_ad001


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_B6_ad006


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_B5_ad010


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_B4_ad007


title: mets participant 9, adipose tissue, pre meal 0 hours, soy meal intervention

type: RNA

city: Notting Hill

first: Aimee

last: Dordevic

state: VIC

address: Level 1, 264 Ferntree Gully Rd

department: Nutrition, Dietetics & Food

country: Australia

institute: Monash University

hyb_protocol: Labelled cRNA samples were hybridized to Illumina Human WG-6 v3 arrays overnight

scan_protocol: Washed and acanned on the Illumina iScan, then the resulting image files were analysed using Illumina Beadstudio software to annotate the genes and generate quantitative expression scores with mean, standard deviation and statistical evaluation of detection reliability averaged across the 30-50 built in technical replicates for each transcript.

data_processing: Analysed using Illumina Beadstudio software; Data were pre-processed using the functions of the R/Bioconductor packages lumi and limma in ArrayAnalysis (http://arrayanalysis.org/), background was subtracted and data were quantile normalised.

label: biotin

label_protocol: According to Illumina protocol; RNA was reverse transcribed using a T7-promoter-oligo (dT) primer, followed by an in vitro transcription reaction with biotin-labeled nucleotides

extract_protocol: RNA was extracted using miRNeasy Mini Kit following the manufactures protocol, and on-column DNase treatment (Qiagen) was performed.

treatment_protocol: A randomised, crossover, single meal study. Participants were provided with a standarised evening meal, fasted overnight, then presented at the laboratory between 7-8am. The participants rested for 30 minutes then an adipose tissue sample was collected from the lateral periumbilical region of the subcutaneous abdominal area. Adipose tissue from each biopsy was washed in ice-cold phosphate-buffered saline (PBS) to eliminate blood and immediately frozen and stored in liquid nitrogen. Participants consumed either a dairy-based or a soy-based, high-fat breakfast within 15 minutes. Postprandial adipose tissue biopsies were collected at  4 hours.

tag: disease

value: metabolic syndrome

value: adipose

tag: Sex


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_B4_ad010

value: SSp


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_B4_ad002


title: mets participant 9, adipose tissue, post meal 4 hours, soy meal intervention


title: mets participant 8, adipose tissue, post meal 4 hours, soy meal intervention


title: mets participant 9, adipose tissue, pre meal 0 hours, dairy meal intervention


title: mets participant 9, adipose tissue, post meal 4 hours, dairy meal intervention


title: mets participant 8, adipose tissue, pre meal 0 hours, soy meal intervention


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_B3_ad012


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_B4_ad001


title: mets participant 7, adipose tissue, post meal 4 hours, soy meal intervention


title: mets participant 7, adipose tissue, pre meal 0 hours, soy meal intervention


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_B5_ad008


title: mets participant 6, adipose tissue, post meal 4 hours, soy meal intervention


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_B3_ad007


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_B3_ad010


title: mets participant 6, adipose tissue, pre meal 0 hours, dairy meal intervention


title: 190425_CEMBA_mm_P53_P63_RS2_190425_P1_190425_P2_B2_ad010