title: SCGC1373_mTEChi_EedZsGpos_K6


title: SCGC1373_mTEChi_EedZsGpos_K5

status: Public on Feb 19 2021

type: SRA

city: Oxford

first: Adam

last: Handel

address: John Radcliffe Hospital

department: Weatherall Institute of Molecular Medicine

country: United Kingdom

institute: University of Oxford

library_source: transcriptomic

data_processing:
QC was performed using FastQC and Trimmomatic
RNA-seq reads were aligned using HISAT (version 0.1.6) and ChIP-seq peaks were pre-aligned using BWA (version 0.5.9) and Stampy (--bamkeepgoodreads)
Single cell RNA-seq outliers were identified using robust PCA on alignment proportion, ERCC92 spike-in proportion, number of detectable genes, proportion of reads mapping to protein-coding genes, proportion of mitochondrial transcripts, proportion of ribosomal transcripts, 3’ to 5’ coverage bias, transcriptomic variance, cell-to-mean correlation, the proportion of the library accounted for by the top 500 transcripts and GC content
RNA-seq reads were assigned to genes using HTSeq (intersection non-empty)
ChIP-seq bigWig profiles were generated using MACS2 on deduplicated BAM files with MAPQ >10
Genome_build: mm10
Supplementary_files_format_and_content: Tab delimited files include counts for RNA-seq samples and bigWig files show ChIP/input signal for ChIP-seq samples

molecule: polyA RNA

organism: Mus musculus

source_name: mTEChi

extract_protocol:
Thymic lobes were cleaned from fatty tissues and incubated with Liberase and DNaseI (Roche Diagnostics; 200μg/ml and 30μg/ml, respectively; 45min, 37ºC) to obtain a cell suspension which was subsequently filtered through a nylon mesh (100micro pore size, Sefar Nitex) to remove debris. TEC were sorted and then RNA was either isolated from ~1,000 cells or single cells using the SMART-seq2 protocol. TEC for ChIP-seq were treated as detailed in Žuklys S et al. Nature Immunology 2016:17(10):1206-1215
RNA-seq libraries were prepared using the appropriate Illumina kit for SMART-seq2 or HiSeq2500 (ChIP-seq). Nextera adapters were used for single cell RNA-seq and ChIP-seq.

tag: strain

tag: sample type

value: single cell

tag: zsgreen status

value: positive


title: SCGC1373_mTEChi_EedZsGpos_K4


title: reanalysis blood_T_718_day-7

city: Vienna

first: Georg

last: Stary

address: Spitalgasse 23

department: Dermatology

country: Austria

institute: Medical University of Vienna

data_processing:
Single-end reads were trimmed for low quality bases and removal of low-quality reads using ReadTools
Trimmed reads were mapped to the Homo sapiens genome (GRCh38.92 assembly) using STAR (v2.5.3a) mapper
The reads mapped in multiple genomic locations were eliminated by using Samtools (v1.4)
Read counts for features (exons) were generated using the featureCounts function from the Subread package (v 1.22.1)
Genome_build: hg38
Supplementary_files_format_and_content: Raw gene count matrix was generated using featureCounts function from the Subread package (v 1.22.1) with Ensembl Known Gene models (version GRCh38.92) as reference annotations

molecule: total RNA

organism: Homo sapiens

source_name: T cells

growth_protocol: freshly isolated cells from human tissue

extract_protocol:
cells were isolated from heparinized blood using ficoll gradient and from skin biopsies using collagenase IV + mesh filtering
Illumina
SMART-seq

tag: tissue

value: blood

tag: time

value: Baseline


title: SCGC1373_mTEChi_EedZsGpos_K3


title: reanalysis blood_T_713_day-7


title: reanalysis blood_T_710_day-7


title: reanalysis blood_T_712_day-7


title: SCGC1373_mTEChi_EedZsGpos_K22


title: SCGC1373_mTEChi_EedZsGpos_K21


title: reanalysis blood_T_717_day-7


title: SCGC1373_mTEChi_EedZsGpos_K23


title: RNA_fPSCs_ROSAmTmG_P12

city: Beijing

first: Wei

last: Xie

address: Haidian District

department: School of Life Science

country: China

institute: Tsinghua University

description:
Bulk RNA-seq
RNA.FPKM.txt

data_processing:
Bulk RNA-seq: Low quality sequencing reads and adapters were removed by cutadapt v1.11, and then the clean reads were aligned to mouse cDNA database (Ensembl, mm9) using TopHat2 v2.1.1. FPKM (Fragments per Kilo-base per Million) was introduced to calculate gene expression value by Cufflinks v2.1.1.
ChIP-seq: Sequencing reads were aligned to mouse genome reference (mm9) using Bowtie2 (v. 2.2.2) with the parameters: --t --q --N 1 --L 25 after trimming adapters by Trim Galore. Multiple mapped reads and PCR duplicates were removed to generate uniquely mapped reads. After validating reproducibility, replicates were integrated for down-stream analysis. The reads per kilobase of transcript per million mapped reads (RPKM) was used to calculate the enrichment of histone modifications at the whole genome level.
MethylC-seq: After trimming low quality sequencing reads and adapters, the clean reads were mapped to mm9 by bs_seeker2 following the default parameters. Unconverted reads, multiple mapped reads and duplicate reads introduced by PCR were also removed.
Single-cell RNA-seq: After trimming adapters and low quality sequencing reads by Trim Galore, the single-cell RNA-seq data of paired-end reads was processed by the standard pipeline of Drop-seq tools-2.0.0.
Genome_build: mm9 (MGSCv37)
Supplementary_files_format_and_content: The bedgraph files for STAR ChIP-seq contain the RPKM value in 100bp bin across the mm9 genome. For STEM-seq, the bed files contain mCG/CG level within 100-bp windows across the whole genome. The RNA.FPKM.txt file of RNA-seq includes FPKM for each sample. scRNA_fPSCs_count.txt files contain the UMI counts of exons of Ensembl genes for 192 single cells.

source_name: stem cells

growth_protocol: Mouse ESCs were cultured on feeder-free dishes coated with 0.2% gelatin (Millipore, Cat# 901771) in the N2B27 medium supplemented with 1 mM PD035901 (Cat# P-9688), 3 mM Chir99021 (Cat# C-6556), and 1e3 units/ml hLIF (human Leukemia Inhibitory Factor, Millipore, Cat# ESG1107). EpiSCs were cultured on mouse MEFs (Embryonic Fibroblast cells) inactivated with Mitomycin C (Sigma-Aldrich, Cat# M0503) in N2B27 medium containing 15% KSR (Gibco, Cat# 10828028), 20 ng/ml Activin A (PeproTech, Cat# 100-18B), 12 ng/ml bFGF2 (R&D Systems, Cat# 233-FB) and 5 mM XAV939 (Sigma-Aldrich, Cat# X3004). The stabilized Epiblastoids (fPSCs) were cultured on Matrigel in N2B27 medium supplemented with 20 ng/ml Activin A, 12 ng/ml bFgf2, and 5 mM XAV939.

extract_protocol: Bulk RNA-seq: Total RNA was isolated from each cell line by RNA-zol. Genomic DNA was eliminated by the digestion of RQ1 DNase (Promega, Cat# M610A) for 1 h at 37 °C. 3ug total RNA was used to construct the RNA sequencing libraries though NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, E7530L) following the manufacturer’s manuals.

tag: genotype

value: WT

tag: cell type source

value: stem cells


title: scRNA_fPSCs (clones3-4)

description:
Single-cell RNA-seq
scRNA_fPSCs_clones3_4_count.txt
scRNA_fPSCs_barcode.csv

extract_protocol: Single-cell RNA-seq: Single cells were obtained from four fPSCs clones after digestion of TrypLE at 37°C for 5 min. Totally, 192 single cells (48 cells/clone) were transferred into the PCR tubes that contained 2.5 ul lysis buffer by mouth pipetting. And then the transcriptome library construction was performed using Smart-seq2 according to the protocol described previously.


title: RNA_fPSCs_CMT_P11

description:
Bulk RNA-seq
RNA.FPKM.txt


title: RNA_fPSCs_BVSC_P14

description:
Bulk RNA-seq
RNA.FPKM.txt


title: SCGC1373_mTEChi_EedZsGpos_K2


title: RNA_fPSCs_46C_P24

description:
Bulk RNA-seq
RNA.FPKM.txt


title: SCGC1373_mTEChi_EedZsGpos_K18


title: SCGC1373_mTEChi_EedZsGpos_K16


title: MethylC_fPSCs_P10_rep2

library_source: genomic

data_processing:
library strategy: MethylC-seq
STAR ChIP-seq datasets were mapped to the mouse mm9 reference genome using Bowtie2 (version 2.2.2) with the parameter:–t –q –N 1 –L 25. Multiple mapped reads and PCR duplicates were removed. After validating the reproducibility, replicates were pooled for downstream analysis.
RNA-seq datasets were mapped to the mouse mm9 reference genome by Tophat 2.1.1. Gene expression was then calculated according to refFlat database by cufflinks 2.1.1.
STEM-seq datasets were trimmed adaptors by Trim galore and then aligned to the mm9 reference genome though BSSeeker2 with the parameters: --bt2-p 8 --XS 0.2,3 --a CCCCCC --m 4. Multiple mapped and duplicated reads were also removed.
Genome_build: mm9
Supplementary_files_format_and_content: The bedgraph files for STAR ChIP-seq contains the RPKM value in 100bp bin across the mm9 genome. For STEM-seq, the bed files contains mCG/CG level within 100-bp windows across the whole genome. The txt file of RNA-seq contains FPKM for each sample.

molecule: genomic DNA

growth_protocol: The finalized medium for fPSCs was N2B27 medium supplemented with 20 ng/ml Activin A, 12 ng/ml bFgf2, and 5 uM XAV939.

extract_protocol:
The DNA extraction follows MethylC-seq protocol.
The MethylC-seq libraries were generated following the protocol as described previously.


title: MethylC_fPSCs_P10_rep1


title: ChIP_fPSCs_P10_H3K27me3_rep2

data_processing:
STAR ChIP-seq datasets were mapped to the mouse mm9 reference genome using Bowtie2 (version 2.2.2) with the parameter:–t –q –N 1 –L 25. Multiple mapped reads and PCR duplicates were removed. After validating the reproducibility, replicates were pooled for downstream analysis.
RNA-seq datasets were mapped to the mouse mm9 reference genome by Tophat 2.1.1. Gene expression was then calculated according to refFlat database by cufflinks 2.1.1.
STEM-seq datasets were trimmed adaptors by Trim galore and then aligned to the mm9 reference genome though BSSeeker2 with the parameters: --bt2-p 8 --XS 0.2,3 --a CCCCCC --m 4. Multiple mapped and duplicated reads were also removed.
Genome_build: mm9
Supplementary_files_format_and_content: The bedgraph files for STAR ChIP-seq contains the RPKM value in 100bp bin across the mm9 genome. For STEM-seq, the bed files contains mCG/CG level within 100-bp windows across the whole genome. The txt file of RNA-seq contains FPKM for each sample.

extract_protocol:
The DNA extraction follows STAR ChIP-seq protocol.
The STAR ChIP-seq libraries were generated following the protocol as described previously(Zhang et al., 2016).

tag: chip antibody


title: SCGC1373_mTEChi_EedZsGpos_K17


title: SCGC1373_mTEChi_EedZsGpos_K15


title: ChIP_fPSCs_P10_H3K27me3_rep1


title: SCGC1373_mTEChi_EedZsGpos_K14


title: ChIP_fPSCs_P10_H3K4me3_rep2

value: H3K4me3(home made)


title: SCGC1373_mTEChi_EedZsGpos_K24


title: SCGC1373_mTEChi_EedZsGpos_K13


title: ChIP_fPSCs_P10_H3K4me3_rep1


title: scRNA_fPSCs (clones1-2)

description:
Single-cell RNA-seq
scRNA_fPSCs_clones1_2_count.txt
scRNA_fPSCs_barcode.csv


title: RNA_E6.5_fPSCs_P10

extract_protocol:
The RNA extraction follows total RNA-seq protocol.
The RNA-seq libraries were generated following the total RNA-seq protocol.


title: SCGC1373_mTEChi_EedZsGpos_K12


title: SCGC1373_mTEChi_EedZsGpos_K10


title: SCGC1373_mTEChi_EedZsGpos_K11


title: SCGC1373_mTEChi_EedZsGpos_K20


title: RNA_E5.5_fPSCs_P10


title: RNA_fPSCs_P30


title: RNA_fPSCs_P20


title: SCGC1373_mTEChi_EedZsGpos_K1


title: RNA_fPSCs_P10


title: SCGC1373_mTEChi_EedZsGpos_J8


title: SCGC1373_mTEChi_EedZsGpos_J7


title: RNA_fPSCs_P1


title: SCGC1373_mTEChi_EedZsGpos_J9


title: SCGC1373_mTEChi_EedZsGpos_K19


title: RNA_Epiblastoids_day3

growth_protocol: Epiblastoids were embedded in Matrigel and  cultured in N2B27 medium supplemented with 1% KSR, 20 ng/ml Activin A and 12 ng/ml bFgf2.


title: SCGC1373_mTEChi_EedZsGpos_J6


title: RNA_Epiblastoids_day2


title: SCGC1373_mTEChi_EedZsGpos_J4


title: RNA_EpiSCs_rep2

growth_protocol: EpiSCs were cultured on mouse MEFs (Embryonic Fibroblast cells) inactivated with Mitomycin C (Sigma-Aldrich, Cat# M0503) in N2B27 medium contained 15% KSR (Gibco, Cat# 10828028), 20 ng/ml Activin A (PeproTech, Cat# 100-18B), 12 ng/ml bFGF2 (R&D Systems, Cat# 233-FB) and 5 uM XAV939 (Sigma-Aldrich, Cat# X3004).


title: SCGC1373_mTEChi_EedZsGpos_J3


title: RNA_EpiSCs_rep1


title: SCGC1373_mTEChi_EedZsGpos_J5


title: SCGC1373_mTEChi_EedZsGpos_J24


title: SCGC1373_mTEChi_EedZsGpos_J23


title: RNA_EpiLCs_rep2

growth_protocol: EpiLCs were cultured in N2B27 medium supplemented with 1% KSR, 20 ng/ml Activin A and 12 ng/ml bFgf2.


title: SCGC1373_mTEChi_EedZsGpos_J22


title: RNA_EpiLCs_rep1


title: RNA_mESCs_rep2

growth_protocol: Mouse ESCs were cultured on feeder-free dishes coated with 0.2% gelatin (Millipore, Cat# 901771) in the N2B27 2i/lif medium, which included N2B27 medium supplemented with 1 uM PD035901 (LC Laboratories, Cat# P-9688), 3 uM Chir99021 (LC Laboratories, Cat# C-6556), and 1000 units/ml hLIF (human Leukemia Inhibitory Factor, Millipore, Cat# ESG1107).


title: SCGC1373_mTEChi_EedZsGpos_J21


title: RNA_mESCs_rep1


title: SCGC1373_mTEChi_EedZsGpos_J2


title: SCGC1373_mTEChi_EedZsGpos_J20


title: SCGC1373_mTEChi_EedZsGpos_J19


title: MDCK infection rep1 - uninfected total RNA

city: Minneapolis

first: Wei-Shou

last: Hu

state: MN

address: 421 Washington Ave. SE

country: USA

institute: University of Minnesota

description:
PR8_infection_FPKM.xlsx
PR8 _t1_UI

data_processing:
All the raw RNA-Seq reads were first trimmed off adapters and removed low-quality bases by Trimmomatic and checked for quality using FASTQC
All the trimmed reads with a minimum read length of 38bp and average quality per base higher than 30 were mapped into the combined genome of host+IAV by STAR v2.5.3a
The expression level of all the genes, including host and viral genes, were quantified by Cufflinks
Genome_build: CanFam3.1 for MDCK cells, GRCh38.p13 for A549 cells, and NCBI Taxon ID 211044 for IAV PR/8/34
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each sample

organism: Canis lupus familiaris

source_name: MDCK cells

growth_protocol: Madin-Darby canine kidney (MDCK) and Human lung adenocarcinoma epithelial A549 cells were maintained in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% FBS. Recombinant A/Puerto Rico/8/34 (H1N1) was rescued by plasmid-based transfection, plaque purified and propagated in embryonic chicken eggs.  The single-cycle virus (Delta-HA and Delta-PB1) were created by replacing the coding sequence of HA and PB1 by mCherry and amplified in MDCK cells engineered to express HA and PB1, respectively. Viral titers were determined by plaque assay in MDCK cells.

extract_protocol:
MDCK total RNA were extracted using RNeasy Mini Kit (QIAGEN). A549 total RNA were extracted using TRIzol.
MDCK cDNA libraries were prepared using the Clontech Pico SMARTer Stranded Total RNA-Seq kit v2. For the third MDCK infection, the RNA was also prepped by Illumina TruSeq Stranded mRNA Rrep kit to isolate mRNA only. A549 cDNA libraries were prepared using the Stranded Total RNA v2 PicoMammalian kit (Takara).

treatment_protocol: MDCK cells were infected with IAV PR8 (MOI =0.5) and incubated at 4ºC for 30 minutes to allow for the virus to adsorb to the cell surface but not to enter the cytosol. Synchronized IAV infection was initiated by shifting the temperature to 37ºC. Infections with single-cycle viruses (Delta-HA and Delta-PB1) were performed in A549 cells in infection media at an MOI of 1. Infections were also synchronized at 4ºC for 30 minutes then incubated at 37ºC.

tag: cell type

value: immortalized kidney epithelial cells

tag: cell line

value: MDCK

tag: treatment

value: uninfected


title: SCGC1373_mTEChi_EedZsGpos_J17


title: RNA_Epiblastoids_day4


title: CEMBA190108_6A_3_CEMBA190108_6A_4_D12_ad010

city: La Jolla

first: Joseph

middle: R

last: Ecker

state: CA

address: 10010 North Torrey Pines Road

department: Genomic Analysis Laboratory

institute: HHMI-Salk-Institute

data_processing:
Library strategy: snmC-seq2
Reads trimed with Cutadapt for adapters and low quality bases (Phred score >= 20)
The resulting files are mapped to in-silico bisulfite converted mm10 reference genome using Bismark.
The raw BAM file will be filtered using samtools to filter reads by MAPQ > 10 and use Picard to remove PCR duplicates
Tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls will be generated with in-house developing package using the filtered BAM files
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls. Columns are: 1. Chromosome, 2. Position, 3. Strand, 4. Cytosin Context, 5. methylated base call, 6. total mapped base call, 7. not used, place holder column

source_name: C57BL/6J Male P56 Mouse Brain

growth_protocol: All mice are C57BL/6J Male, brain dissected in P56

extract_protocol:
the brain of P56 male C57BL/6J mouse will be removed and cut into 600 microns thick coronal sections using a tissue matrix. Then each region will be microdissected from the coronal tissue sections based on identified landmarks and brain atlas coordinates, as defined in Allen brain atlas CCF3 (Avaliable at: http://atlas.brain-map.org). For each dissection region, the tissue from several littermate mice will be combined together into to separate replicate groups. The number of animals in a replicate depends on the size of the tissue. Nuclei preparation will be stained with the fluorescently labeled anti-NeuN body and nucleic acid stain Hoechst 33342.
Library prepared following protocol discribed in Luo, Chongyuan, Angeline Rivkin, Jingtian Zhou, Justin P. Sandoval, Laurie Kurihara, Jacinta Lucero, Rosa Castanon, et al. 2018. “Robust Single-Cell DNA Methylome Profiling with snmC-seq2.” Nature Communications 9 (1): 3824.

tag: brain region

value: ACA,PL,ILA

tag: Sex

value: male

tag: age


title: CEMBA190108_6A_3_CEMBA190108_6A_4_D12_ad008


title: SCGC1373_mTEChi_EedZsGpos_J18


title: CEMBA190108_6A_3_CEMBA190108_6A_4_D12_ad007


title: CEMBA190108_6A_3_CEMBA190108_6A_4_D12_ad004


title: CEMBA190108_6A_3_CEMBA190108_6A_4_D12_ad006


title: SCGC1373_mTEChi_EedZsGpos_J16


title: SCGC1373_mTEChi_EedZsGpos_J14


title: SCGC1373_mTEChi_EedZsGpos_J15


title: CEMBA190108_6A_3_CEMBA190108_6A_4_D12_ad002


title: SCGC1373_mTEChi_EedZsGpos_J13


title: CEMBA190108_6A_3_CEMBA190108_6A_4_D11_ad010


title: CEMBA190108_6A_3_CEMBA190108_6A_4_D12_ad001


title: SCGC1373_mTEChi_EedZsGpos_J11


title: CEMBA190108_6A_3_CEMBA190108_6A_4_D11_ad008


title: SCGC1373_mTEChi_EedZsGpos_J12


title: SCGC1373_mTEChi_EedZsGpos_J10


title: CEMBA190108_6A_3_CEMBA190108_6A_4_D11_ad007


title: CEMBA190108_6A_3_CEMBA190108_6A_4_D11_ad006


title: SCGC1373_mTEChi_EedZsGpos_J1


title: CEMBA190108_6A_3_CEMBA190108_6A_4_D11_ad004


title: SCGC1373_mTEChi_EedZsGpos_I8


title: CEMBA190108_6A_3_CEMBA190108_6A_4_D11_ad002


title: SCGC1373_mTEChi_EedZsGpos_I9


title: SCGC1373_mTEChi_EedZsGpos_I7


title: CEMBA190108_6A_3_CEMBA190108_6A_4_D11_ad001


title: CEMBA190108_6A_3_CEMBA190108_6A_4_D10_ad012


title: SCGC1373_mTEChi_EedZsGpos_I6


title: CEMBA190108_6A_3_CEMBA190108_6A_4_D10_ad006


title: SCGC1373_mTEChi_EedZsGpos_I3


title: CEMBA190108_6A_3_CEMBA190108_6A_4_D10_ad007


title: CEMBA190108_6A_3_CEMBA190108_6A_4_D10_ad008


title: SCGC1373_mTEChi_EedZsGpos_I24


title: CEMBA190108_6A_3_CEMBA190108_6A_4_D10_ad004


title: SCGC1373_mTEChi_EedZsGpos_I5


title: SCGC1373_mTEChi_EedZsGpos_I23


title: SCGC1373_mTEChi_EedZsGpos_I21


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C9_ad008


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C9_ad002


title: CEMBA190108_6A_3_CEMBA190108_6A_4_D10_ad002


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C9_ad006


title: CEMBA190108_6A_3_CEMBA190108_6A_4_D10_ad001


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C9_ad004


title: SCGC1373_mTEChi_EedZsGpos_I2


title: SCGC1373_mTEChi_EedZsGpos_I19


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C9_ad001


title: SCGC1373_mTEChi_EedZsGpos_I18


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C9_ad012


title: SCGC1373_mTEChi_EedZsGpos_I17


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C8_ad012


title: SCGC1373_mTEChi_EedZsGpos_I16


title: SCGC1373_mTEChi_EedZsGpos_I15


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C8_ad010


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C8_ad004


title: SCGC1373_mTEChi_EedZsGpos_I4


title: SCGC1373_mTEChi_EedZsGpos_I12


title: SCGC1373_mTEChi_EedZsGpos_I11


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C8_ad002


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C8_ad008


title: SCGC1373_mTEChi_EedZsGpos_I14


title: SCGC1373_mTEChi_EedZsGpos_I20


title: SCGC1373_mTEChi_EedZsGpos_I22


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C7_ad001


title: SCGC1373_mTEChi_EedZsGpos_I10


title: SCGC1373_mTEChi_EedZsGpos_I1


title: SCGC1373_mTEChi_EedZsGpos_H9


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C7_ad010


title: SCGC1373_mTEChi_EedZsGpos_H8


title: SCGC1373_mTEChi_EedZsGpos_H7


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C7_ad006


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C7_ad002


title: SCGC1373_mTEChi_EedZsGpos_I13


title: SCGC1373_mTEChi_EedZsGpos_H6


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C6_ad012


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C7_ad008


title: SCGC1373_mTEChi_EedZsGpos_H5


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C6_ad001


title: SCGC1373_mTEChi_EedZsGpos_H4


title: SCGC1373_mTEChi_EedZsGpos_H3


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C6_ad007


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C7_ad012


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C6_ad002


title: SCGC1373_mTEChi_EedZsGpos_H24


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C6_ad004


title: SCGC1373_mTEChi_EedZsGpos_H23


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C6_ad008


title: SCGC1373_mTEChi_EedZsGpos_H21


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C7_ad007


title: SCGC1373_mTEChi_EedZsGpos_H22


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C5_ad010


title: SCGC1373_mTEChi_EedZsGpos_H20


title: SCGC1373_mTEChi_EedZsGpos_H19


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C5_ad001


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C5_ad004


title: SCGC1373_mTEChi_EedZsGpos_H18


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C5_ad007


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C4_ad012


title: SCGC1373_mTEChi_EedZsGpos_H17


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C5_ad006


title: SCGC1373_mTEChi_EedZsGpos_H16


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C4_ad007


title: SCGC1373_mTEChi_EedZsGpos_H13


title: SCGC1373_mTEChi_EedZsGpos_H12


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C4_ad006


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C4_ad002


title: SCGC1373_mTEChi_EedZsGpos_H11


title: SCGC1373_mTEChi_EedZsGpos_H10


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C4_ad004


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C3_ad012


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C4_ad008


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C3_ad001


title: SCGC1373_mTEChi_EedZsGpos_H1


title: SCGC1373_mTEChi_EedZsGpos_G9


title: SCGC1373_mTEChi_EedZsGpos_H2


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C5_ad002


title: SCGC1373_mTEChi_EedZsGpos_G8


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C3_ad007


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C3_ad010


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C3_ad006


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C4_ad001


title: SCGC1373_mTEChi_EedZsGpos_G4


title: SCGC1373_mTEChi_EedZsGpos_H15


title: SCGC1373_mTEChi_EedZsGpos_H14


title: SCGC1373_mTEChi_EedZsGpos_G24


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C4_ad010


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C3_ad002


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C3_ad004


title: SCGC1373_mTEChi_EedZsGpos_G7


title: SCGC1373_mTEChi_EedZsGpos_G23


title: SCGC1373_mTEChi_EedZsGpos_G22


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C2_ad012


title: SCGC1373_mTEChi_EedZsGpos_G5


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C3_ad008


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C2_ad001


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C2_ad007


title: SCGC1373_mTEChi_EedZsGpos_G21


title: SCGC1373_mTEChi_EedZsGpos_G20


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C2_ad006


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C2_ad010


title: SCGC1373_mTEChi_EedZsGpos_G6


title: SCGC1373_mTEChi_EedZsGpos_G18


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C2_ad004


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C2_ad002


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C2_ad008


title: SCGC1373_mTEChi_EedZsGpos_G16


title: SCGC1373_mTEChi_EedZsGpos_G14


title: SCGC1373_mTEChi_EedZsGpos_G17


title: SCGC1373_mTEChi_EedZsGpos_G11


title: SCGC1373_mTEChi_EedZsGpos_G12


title: SCGC1373_mTEChi_EedZsGpos_G13


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C12_ad010


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C12_ad012


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C12_ad008


title: SCGC1373_mTEChi_EedZsGpos_G10


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C12_ad007


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C12_ad006


title: SCGC1373_mTEChi_EedZsGposBulk100_G3

value: small bulk


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C12_ad004


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C12_ad002


title: SCGC1373_mTEChi_EedZsGposBulk100_G2


title: SCGC1373_mTEChi_EedZsGneg_F9

value: negative


title: SCGC1373_mTEChi_EedZsGposBulk100_G1


title: SCGC1373_mTEChi_EedZsGpos_G19


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C11_ad012


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C11_ad010


title: SCGC1373_mTEChi_EedZsGpos_G15


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C11_ad007


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C11_ad008


title: SCGC1373_mTEChi_EedZsGneg_F7


title: SCGC1373_mTEChi_EedZsGneg_F8


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C11_ad006


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C11_ad004


title: SCGC1373_mTEChi_EedZsGneg_F6


title: SCGC1373_mTEChi_EedZsGneg_F4


title: SCGC1373_mTEChi_EedZsGneg_F5


title: SCGC1373_mTEChi_EedZsGneg_F3


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C11_ad002


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C11_ad001


title: SCGC1373_mTEChi_EedZsGneg_F24


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C10_ad012


title: SCGC1373_mTEChi_EedZsGneg_F23


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C12_ad001


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C10_ad010


title: SCGC1373_mTEChi_EedZsGneg_F22


title: SCGC1373_mTEChi_EedZsGneg_F21


title: SCGC1373_mTEChi_EedZsGneg_F2


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C10_ad002


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C10_ad008


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C10_ad004


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C10_ad006


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C10_ad007


title: SCGC1373_mTEChi_EedZsGneg_F19


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C10_ad001


title: SCGC1373_mTEChi_EedZsGneg_F17


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C1_ad001


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C1_ad007


title: SCGC1373_mTEChi_EedZsGneg_F14


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C1_ad010


title: SCGC1373_mTEChi_EedZsGneg_F13


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C1_ad006


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C1_ad004


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B9_ad012


title: SCGC1373_mTEChi_EedZsGneg_F10


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C1_ad002


title: SCGC1373_mTEChi_EedZsGneg_F20


title: SCGC1373_mTEChi_EedZsGneg_F1


title: SCGC1373_mTEChi_EedZsGneg_E9


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B9_ad001


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B9_ad007


title: SCGC1373_mTEChi_EedZsGneg_E8


title: SCGC1373_mTEChi_EedZsGneg_F18


title: SCGC1373_mTEChi_EedZsGneg_F16


title: SCGC1373_mTEChi_EedZsGneg_E7


title: CEMBA190108_6A_3_CEMBA190108_6A_4_C1_ad012


title: SCGC1373_mTEChi_EedZsGneg_F15


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B9_ad006


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B9_ad004


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B9_ad010


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B9_ad002


title: SCGC1373_mTEChi_EedZsGneg_E6


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B9_ad008


title: SCGC1373_mTEChi_EedZsGneg_F11


title: SCGC1373_mTEChi_EedZsGneg_E3


title: SCGC1373_mTEChi_EedZsGneg_E4


title: SCGC1373_mTEChi_EedZsGneg_E24


title: SCGC1373_mTEChi_EedZsGneg_F12


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B8_ad012


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B8_ad001


title: SCGC1373_mTEChi_EedZsGneg_E23


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B8_ad007


title: SCGC1373_mTEChi_EedZsGneg_E22


title: SCGC1373_mTEChi_EedZsGneg_E21


title: SCGC1373_mTEChi_EedZsGneg_E20


title: SCGC1373_mTEChi_EedZsGneg_E2


title: SCGC1373_mTEChi_EedZsGneg_E5


title: SCGC1373_mTEChi_EedZsGneg_E19


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B8_ad004


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B7_ad012


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B7_ad001


title: SCGC1373_mTEChi_EedZsGneg_E18


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B7_ad006


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B7_ad010


title: SCGC1373_mTEChi_EedZsGneg_E15


title: SCGC1373_mTEChi_EedZsGneg_E14


title: SCGC1373_mTEChi_EedZsGneg_E16


title: SCGC1373_mTEChi_EedZsGneg_E17


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B7_ad002


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B7_ad008


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B7_ad004


title: SCGC1373_mTEChi_EedZsGneg_E13


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B6_ad012


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B6_ad001


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B8_ad010


title: SCGC1373_mTEChi_EedZsGneg_E12


title: SCGC1373_mTEChi_EedZsGneg_E11


title: SCGC1373_mTEChi_EedZsGneg_E10


title: SCGC1373_mTEChi_EedZsGneg_E1


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B6_ad007


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B6_ad010


title: SCGC1373_mTEChi_EedZsGneg_D9


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B8_ad002


title: SCGC1373_mTEChi_EedZsGneg_D8


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B6_ad004


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B6_ad006


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B6_ad002


title: SCGC1373_mTEChi_EedZsGneg_D7


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B6_ad008


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B5_ad001


title: SCGC1373_mTEChi_EedZsGneg_D5


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B5_ad006


title: SCGC1373_mTEChi_EedZsGneg_D4


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B5_ad004


title: SCGC1373_mTEChi_EedZsGneg_D24


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B5_ad002


title: SCGC1373_mTEChi_EedZsGneg_D22


title: SCGC1373_mTEChi_EedZsGneg_D23


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B5_ad008


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B4_ad007


title: SCGC1373_mTEChi_EedZsGneg_D21


title: SCGC1373_mTEChi_EedZsGneg_D20


title: SCGC1373_mTEChi_EedZsGneg_D2


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B4_ad006


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B4_ad004


title: SCGC1373_mTEChi_EedZsGneg_D19


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B4_ad008


title: SCGC1373_mTEChi_EedZsGneg_D6


title: SCGC1373_mTEChi_EedZsGneg_D18


title: SCGC1373_mTEChi_EedZsGneg_D17


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B4_ad010


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B3_ad007


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B3_ad010


title: SCGC1373_mTEChi_EedZsGneg_D3


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B3_ad001


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B3_ad006


title: SCGC1373_mTEChi_EedZsGneg_D14


title: SCGC1373_mTEChi_EedZsGneg_D13


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B3_ad012


title: SCGC1373_mTEChi_EedZsGneg_D15


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B3_ad004


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B4_ad012


title: SCGC1373_mTEChi_EedZsGneg_D12


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B3_ad002


title: SCGC1373_mTEChi_EedZsGneg_D11


title: SCGC1373_mTEChi_EedZsGneg_D10


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B3_ad008


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B2_ad012


title: SCGC1373_mTEChi_EedZsGneg_D1


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B2_ad007


title: SCGC1373_mTEChi_EedZsGneg_C9


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B2_ad001


title: SCGC1373_mTEChi_EedZsGneg_C8


title: SCGC1373_mTEChi_EedZsGneg_D16


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B2_ad010


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B2_ad006


title: SCGC1373_mTEChi_EedZsGneg_C5


title: SCGC1373_mTEChi_EedZsGneg_C6


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B2_ad004


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B2_ad002


title: SCGC1373_mTEChi_EedZsGneg_C4


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B12_ad012


title: SCGC1373_mTEChi_EedZsGneg_C23


title: SCGC1373_mTEChi_EedZsGneg_C24


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B12_ad008


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B12_ad010


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B12_ad007


title: SCGC1373_mTEChi_EedZsGneg_C3


title: SCGC1373_mTEChi_EedZsGneg_C21


title: SCGC1373_mTEChi_EedZsGneg_C22


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B12_ad004


title: SCGC1373_mTEChi_EedZsGneg_C20


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B12_ad002


title: SCGC1373_mTEChi_EedZsGneg_C2


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B12_ad006


title: SCGC1373_mTEChi_EedZsGneg_C19


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B12_ad001


title: SCGC1373_mTEChi_EedZsGneg_C7


title: SCGC1373_mTEChi_EedZsGneg_C18


title: SCGC1373_mTEChi_EedZsGneg_C17


title: SCGC1373_mTEChi_EedZsGneg_C16


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B11_ad012


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B11_ad010


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B11_ad008


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B11_ad007


title: SCGC1373_mTEChi_EedZsGneg_C15


title: SCGC1373_mTEChi_EedZsGneg_C13


title: SCGC1373_mTEChi_EedZsGneg_C14


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B11_ad006


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B11_ad002


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B11_ad004


title: SCGC1373_mTEChi_EedZsGneg_C12


title: SCGC1373_mTEChi_EedZsGneg_C11


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B11_ad001


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B10_ad012


title: SCGC1373_mTEChi_EedZsGneg_C10


title: SCGC1373_mTEChi_EedZsGneg_C1


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B10_ad008


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B10_ad010


title: SCGC1373_mTEChi_EedZsGnegBulk100_A3


title: SCGC1373_mTEChi_EedZsGnegBulk100_A2


title: SCGC1373_mTEChi_EedZsGnegBulk100_A1


title: SCGC1373_mTEChi_EedZsGneg_B9


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B10_ad007


title: SCGC1373_mTEChi_EedZsGneg_B8


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B10_ad004


title: SCGC1373_mTEChi_EedZsGneg_B7


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B10_ad002


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B1_ad012


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B1_ad001


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B10_ad001


title: SCGC1373_mTEChi_EedZsGneg_B6


title: SCGC1373_mTEChi_EedZsGneg_B5


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B1_ad007


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B1_ad010


title: SCGC1373_mTEChi_EedZsGneg_B3


title: SCGC1373_mTEChi_EedZsGneg_B24


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B1_ad008


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A9_ad001


title: SCGC1373_mTEChi_EedZsGneg_B22


title: SCGC1373_mTEChi_EedZsGneg_B23


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A9_ad012


title: CEMBA190108_6A_3_CEMBA190108_6A_4_B10_ad006


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A9_ad010


title: SCGC1373_mTEChi_EedZsGneg_B20


title: SCGC1373_mTEChi_EedZsGneg_B21


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A9_ad006


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A9_ad007


title: SCGC1373_mTEChi_EedZsGneg_B19


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A9_ad004


title: SCGC1373_mTEChi_EedZsGneg_B18


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A9_ad008


title: SCGC1373_mTEChi_EedZsGneg_B4


title: SCGC1373_mTEChi_EedZsGneg_B16


title: SCGC1373_mTEChi_EedZsGneg_B17


title: SCGC1373_mTEChi_EedZsGneg_B14


title: SCGC1373_mTEChi_EedZsGneg_B15


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A8_ad010


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A8_ad001


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A8_ad007


title: SCGC1373_mTEChi_EedZsGneg_B13


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A8_ad006


title: SCGC1373_mTEChi_EedZsGneg_B11


title: SCGC1373_mTEChi_EedZsGneg_B12


title: SCGC1373_mTEChi_EedZsGneg_B10


title: SCGC1373_mTEChi_EedZsGneg_B1


title: SCGC1373_mTEChi_EedZsGneg_B2


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A8_ad004


title: SCGC1373_mTEChi_EedZsGneg_A8


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A8_ad008


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A7_ad012


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A7_ad001


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A7_ad007


title: SCGC1373_mTEChi_EedZsGneg_A7


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A7_ad010


title: SCGC1373_mTEChi_EedZsGneg_A9


title: SCGC1373_mTEChi_EedZsGneg_A6


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A7_ad004


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A7_ad002


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A9_ad002


title: SCGC1373_mTEChi_EedZsGneg_A4


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A8_ad012


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A7_ad008


title: SCGC1373_mTEChi_EedZsGneg_A5


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A6_ad012


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A6_ad007


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A6_ad001


title: SCGC1373_mTEChi_EedZsGneg_A21


title: SCGC1373_mTEChi_EedZsGneg_A23


title: SCGC1373_mTEChi_EedZsGneg_A20


title: SCGC1373_mTEChi_EedZsGneg_A18


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A5_ad001


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A6_ad002


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A6_ad004


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A5_ad007


title: SCGC1373_mTEChi_EedZsGneg_A24


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A5_ad006


title: SCGC1373_mTEChi_EedZsGneg_A22


title: SCGC1373_mTEChi_EedZsGneg_A12


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A5_ad008


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A6_ad008


title: SCGC1373_mTEChi_EedZsGneg_A11


title: SCGC1373_mTEChi_EedZsGneg_A17


title: SCGC1373_mTEChi_EedZsGneg_A16


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A6_ad006


title: cTEC_WT_P9

source_name: cTEC

value: Eedflfl


title: SCGC1373_mTEChi_EedZsGneg_A15


title: cTEC_WT_P8


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A5_ad010


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A4_ad010


title: SCGC1373_mTEChi_EedZsGneg_A14


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A5_ad004


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A4_ad006


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A4_ad012


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A6_ad010


title: SCGC1373_mTEChi_EedZsGneg_A13


title: SCGC1373_mTEChi_EedZsGneg_A10


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A4_ad007


title: SCGC1373_mTEChi_EedZsGneg_A19


title: cTEC_WT_P4


title: cTEC_WT_P7


title: cTEC_WT_P21


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A4_ad001


title: cTEC_WT_P6


title: cTEC_WT_P5


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A4_ad002


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A3_ad001


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A3_ad012


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A3_ad004


title: cTEC_WT_P3


title: cTEC_WT_P24


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A3_ad010


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A4_ad004


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A3_ad008


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A3_ad007


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A2_ad001


title: cTEC_WT_P18


title: cTEC_WT_P23


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A3_ad006


title: cTEC_WT_P22


title: cTEC_WT_P15


title: cTEC_WT_P2


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A3_ad002


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A2_ad012


title: cTEC_WT_P17


title: cTEC_WT_P13


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A2_ad007


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A2_ad010


title: cTEC_WT_P19


title: cTEC_WT_P11


title: cTEC_WT_P10


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A2_ad006


title: cTEC_WT_P14


title: cTEC_WT_O9


title: cTEC_WT_P12


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A2_ad002


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A2_ad004


title: cTEC_WT_P1


title: cTEC_WT_O7


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A12_ad012


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A12_ad010


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A2_ad008


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A12_ad006


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A12_ad002


title: cTEC_WT_P16


title: cTEC_WT_O6


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A12_ad008


title: cTEC_WT_O8


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A12_ad004


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A11_ad012


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A12_ad001


title: cTEC_WT_O24


title: cTEC_WT_O5


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A11_ad010


title: cTEC_WT_O4


title: cTEC_WT_O22


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A12_ad007


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A11_ad007


title: cTEC_WT_O21


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A11_ad008


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A11_ad001


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A11_ad004


title: cTEC_WT_P20


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A11_ad006


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A10_ad012


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A10_ad006


title: cTEC_WT_O18


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A10_ad004


title: cTEC_WT_O3


title: cTEC_WT_O16


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A10_ad002


title: cTEC_WT_O17


title: cTEC_WT_O15


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A10_ad001


title: cTEC_WT_O23


title: cTEC_WT_O14


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A1_ad001


title: cTEC_WT_O13


title: cTEC_WT_O12


title: cTEC_WT_O11


title: cTEC_WT_O20


title: cTEC_WT_O10


title: cTEC_WT_O2


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A1_ad008


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A1_ad002


title: cTEC_WT_O19


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A10_ad010


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H9_ad001


title: cTEC_WT_N9


title: cTEC_WT_O1


title: cTEC_WT_N8


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H9_ad012


title: cTEC_WT_N6


title: cTEC_WT_N7


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H9_ad006


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H9_ad010


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A1_ad012


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H9_ad004


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H9_ad002


title: cTEC_WT_N4


title: cTEC_WT_N3


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H9_ad008


title: cTEC_WT_N24


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H8_ad012


title: cTEC_WT_N23


title: CEMBA190108_6A_3_CEMBA190108_6A_4_A1_ad010


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H8_ad001


title: cTEC_WT_N22


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H8_ad006


title: cTEC_WT_N21


title: cTEC_WT_N2


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H8_ad004


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H8_ad002


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H8_ad008


title: cTEC_WT_N20


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H7_ad012


title: cTEC_WT_N19


title: cTEC_WT_N17


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H7_ad001


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H7_ad007


title: cTEC_WT_N16


title: cTEC_WT_N15


title: cTEC_WT_N14


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H7_ad006


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H7_ad002


title: cTEC_WT_N13


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H7_ad010


title: cTEC_WT_N18


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H7_ad004


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H6_ad012


title: cTEC_WT_N10


title: cTEC_WT_N12


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H7_ad008


title: cTEC_WT_N11


title: cTEC_WT_N1


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H6_ad001


title: cTEC_WT_M9


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H6_ad006


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H6_ad010


title: cTEC_WT_M7


title: cTEC_WT_M4


title: cTEC_WT_M23


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H6_ad004


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H6_ad002


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H5_ad008


title: cTEC_WT_M5


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H5_ad004


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H4_ad012


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H5_ad012


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H5_ad002


title: cTEC_WT_M19


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H4_ad001


title: cTEC_WT_M8


title: cTEC_WT_M18


title: cTEC_WT_M17


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H5_ad007


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H4_ad006


title: cTEC_WT_M3


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H5_ad001


title: cTEC_WT_M24


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H5_ad010


title: cTEC_WT_M22


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H5_ad006


title: cTEC_WT_M20


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H6_ad007


title: cTEC_WT_M15


title: cTEC_WT_M14


title: cTEC_WT_M2


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H3_ad006


title: cTEC_WT_M11


title: cTEC_WT_M10


title: cTEC_WT_M12


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H4_ad007


title: cTEC_WT_M21


title: cTEC_WT_M6


title: cTEC_WT_M1


title: cTEC_WT_M16


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H3_ad002


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H4_ad004


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H4_ad008


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H3_ad012


title: cTEC_WT_L9


title: cTEC_WT_L6


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H2_ad007


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H3_ad010


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H3_ad001


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H3_ad004


title: cTEC_WT_L3


title: cTEC_WT_L8


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H2_ad006


title: cTEC_WT_M13


title: cTEC_WT_L7


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H2_ad012


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H2_ad001


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H2_ad004


title: cTEC_WT_L23


title: cTEC_WT_L24


title: cTEC_WT_L22


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H3_ad008


title: cTEC_WT_L21


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H2_ad002


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H12_ad012


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H2_ad008


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H12_ad008


title: cTEC_WT_L20


title: cTEC_WT_L5


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H2_ad010


title: cTEC_WT_L4


title: cTEC_WT_L19


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H12_ad007


title: cTEC_WT_L18


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H12_ad006


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H12_ad002


title: cTEC_WT_L2


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H12_ad010


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H12_ad004


title: cTEC_WT_L17


title: cTEC_WT_L16


title: cTEC_WT_L14


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H12_ad001


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H11_ad007


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H11_ad006


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H11_ad004


title: cTEC_WT_L11


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H11_ad001


title: cTEC_WT_L1


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H10_ad012


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H10_ad010


title: cTEC_WT_K9


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H11_ad002


title: cTEC_WT_L15


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H11_ad008


title: cTEC_WT_L13


title: cTEC_WT_L12


title: cTEC_WT_L10


title: cTEC_WT_K8


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H10_ad007


title: cTEC_WT_K7


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H10_ad008


title: cTEC_WT_K6


title: cTEC_WT_K5


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H10_ad004


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H10_ad006


title: cTEC_WT_K4


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H10_ad002


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H10_ad001


title: cTEC_WT_K23


title: cTEC_WT_K21


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H1_ad007


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H1_ad001


title: cTEC_WT_K22


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H1_ad006


title: cTEC_WT_K3


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H1_ad010


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H1_ad004


title: cTEC_WT_K2


title: cTEC_WT_K20


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H1_ad008


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G9_ad012


title: cTEC_WT_K19


title: cTEC_WT_K18


title: cTEC_WT_K16


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G9_ad001


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G9_ad010


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G9_ad007


title: CEMBA190108_6A_1_CEMBA190108_6A_2_H1_ad012


title: cTEC_WT_K14


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G9_ad006


title: cTEC_WT_K13


title: cTEC_WT_K12


title: cTEC_WT_K11


title: cTEC_WT_K24


title: cTEC_WT_K10


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G9_ad002


title: cTEC_WT_J9


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G8_ad012


title: cTEC_WT_K17


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G9_ad008


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G8_ad007


title: cTEC_WT_K1


title: cTEC_WT_J7


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G8_ad006


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G8_ad010


title: cTEC_WT_J8


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G9_ad004


title: cTEC_WT_K15


title: cTEC_WT_J4


title: cTEC_WT_J5


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G8_ad002


title: cTEC_WT_J3


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G7_ad012


title: cTEC_WT_J24


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G7_ad007


title: cTEC_WT_J23


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G7_ad001


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G8_ad001


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G7_ad010


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G7_ad006


title: cTEC_WT_J21


title: cTEC_WT_J20


title: cTEC_WT_J19


title: cTEC_WT_J6


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G8_ad004


title: cTEC_WT_J2


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G7_ad002


title: cTEC_WT_J18


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G6_ad012


title: cTEC_WT_J17


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G6_ad010


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G6_ad007


title: cTEC_WT_J16


title: cTEC_WT_J22


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G8_ad008


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G6_ad004


title: cTEC_WT_J15


title: cTEC_WT_J14


title: cTEC_WT_J13


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G6_ad001


title: cTEC_WT_J12


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G7_ad004


title: cTEC_WT_J10


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G5_ad012


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G5_ad010


title: cTEC_WT_J1


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G6_ad008


title: cTEC_WT_J11


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G5_ad006


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G5_ad008


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G5_ad002


title: cTEC_WT_I9


title: cTEC_WT_I6


title: cTEC_WT_I8


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G6_ad006


title: cTEC_WT_I7


title: cTEC_WT_I5


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G4_ad012


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G4_ad006


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G4_ad001


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G4_ad010


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G4_ad007


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G6_ad002


title: cTEC_WT_I3


title: cTEC_WT_I23


title: cTEC_WT_I24


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G4_ad002


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G4_ad004


title: cTEC_WT_I21


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G4_ad008


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G3_ad012


title: cTEC_WT_I19


title: cTEC_WT_I20


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G3_ad007


title: cTEC_WT_I4


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G3_ad010


title: cTEC_WT_I2


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G3_ad006


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G3_ad004


title: cTEC_WT_I17


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G3_ad002


title: cTEC_WT_I18


title: cTEC_WT_I15


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G2_ad012


title: cTEC_WT_I16


title: cTEC_WT_I22


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G2_ad001


title: cTEC_WT_I12


title: cTEC_WT_I14


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G2_ad004


title: cTEC_WT_I13


title: cTEC_WT_I11


title: cTEC_WT_I10


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G2_ad002


title: cTEC_WT_I1


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G2_ad007


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G2_ad006


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G3_ad001


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G12_ad012


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G12_ad010


title: cTEC_WT_H7


title: cTEC_WT_H9


title: cTEC_WT_H6


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G12_ad007


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G12_ad006


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G12_ad004


title: cTEC_WT_H5


title: cTEC_WT_H3


title: cTEC_WT_H4


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G12_ad002


title: cTEC_WT_H24


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G11_ad012


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G11_ad010


title: cTEC_WT_H22


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G11_ad004


title: cTEC_WT_H21


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G11_ad006


title: cTEC_WT_H23


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G11_ad007


title: cTEC_WT_H8


title: cTEC_WT_H18


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G11_ad002


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G12_ad008


title: cTEC_WT_H20


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G10_ad012


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G11_ad001


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G10_ad010


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G10_ad008


title: cTEC_WT_H15


title: cTEC_WT_H16


title: cTEC_WT_H2


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G10_ad007


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G12_ad001


title: cTEC_WT_H17


title: cTEC_WT_H14


title: cTEC_WT_H11


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G10_ad006


title: cTEC_WT_H13


title: cTEC_WT_H19


title: cTEC_WT_H10


title: cTEC_WT_H12


title: cTEC_WT_H1


title: cTEC_WT_G9


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G10_ad001


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G10_ad002


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G1_ad010


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G1_ad004


title: cTEC_WT_G8


title: cTEC_WT_G7


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G1_ad006


title: cTEC_WT_G5


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G1_ad008


title: cTEC_WT_G4


title: cTEC_WT_G3


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F9_ad012


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F9_ad001


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F9_ad007


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G10_ad004


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F9_ad010


title: cTEC_WT_G24


title: cTEC_WT_G23


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F9_ad006


title: cTEC_WT_G22


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G1_ad012


title: CEMBA190108_6A_1_CEMBA190108_6A_2_G1_ad007


title: cTEC_WT_G21


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F9_ad004


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F9_ad002


title: cTEC_WT_G20


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F9_ad008


title: cTEC_WT_G2


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F8_ad012


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F8_ad001


title: cTEC_WT_G19


title: cTEC_WT_G18


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F8_ad010


title: cTEC_WT_G6


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F8_ad007


title: cTEC_WT_G16


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F8_ad004


title: cTEC_WT_G15


title: cTEC_WT_G17


title: cTEC_WT_G14


title: cTEC_WT_G13


title: cTEC_WT_G12


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F7_ad010


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F7_ad001


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F8_ad002


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F7_ad007


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F7_ad006


title: cTEC_WT_G1


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F7_ad004


title: cTEC_WT_F9


title: cTEC_WT_F8


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F7_ad008


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F6_ad012


title: cTEC_WT_F6


title: cTEC_WT_F7


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F6_ad007


title: cTEC_WT_F5


title: cTEC_WT_F24


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F6_ad006


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F8_ad008


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F6_ad010


title: cTEC_WT_F4


title: cTEC_WT_F3


title: cTEC_WT_F23


title: cTEC_WT_F22


title: cTEC_WT_G10


title: cTEC_WT_G11


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F6_ad008


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F6_ad004


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F5_ad012


title: cTEC_WT_F21


title: cTEC_WT_F20


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F5_ad007


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F7_ad002


title: cTEC_WT_F2


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F5_ad010


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F6_ad001


title: cTEC_WT_F19


title: cTEC_WT_F18


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F5_ad006


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F5_ad002


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F5_ad004


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F5_ad008


title: cTEC_WT_F16


title: cTEC_WT_F14


title: cTEC_WT_F12


title: cTEC_WT_F13


title: cTEC_WT_F15


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F4_ad006


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F4_ad004


title: cTEC_WT_F11


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F4_ad001


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F4_ad007


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F4_ad002


title: cTEC_WT_F10


title: cTEC_WT_F1


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F4_ad008


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F3_ad012


title: cTEC_WT_E9


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F3_ad001


title: cTEC_WT_E8


title: cTEC_WT_E7


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F3_ad010


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F3_ad007


title: cTEC_WT_E6


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F3_ad006


title: cTEC_WT_E5


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F3_ad004


title: cTEC_WT_F17


title: cTEC_WT_E4


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F3_ad008


title: cTEC_WT_E3


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F2_ad012


title: cTEC_WT_E24


title: cTEC_WT_E22


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F2_ad007


title: cTEC_WT_E23


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F2_ad001


title: cTEC_WT_E21


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F2_ad010


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F2_ad006


title: cTEC_WT_E20


title: cTEC_WT_E2


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F2_ad002


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F2_ad004


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F2_ad008


title: cTEC_WT_E17


title: cTEC_WT_E18


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F12_ad012


title: cTEC_WT_E16


title: cTEC_WT_E19


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F12_ad008


title: cTEC_WT_E15


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F12_ad010


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F12_ad007


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F12_ad004


title: cTEC_WT_E13


title: cTEC_WT_E12


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F12_ad002


title: cTEC_WT_E14


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F12_ad001


title: cTEC_WT_E10


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F11_ad012


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F11_ad010


title: cTEC_WT_E1


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F11_ad008


title: cTEC_WT_D9


title: cTEC_WT_E11


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F11_ad007


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F11_ad006


title: cTEC_WT_D8


title: cTEC_WT_D7


title: cTEC_WT_D6


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F11_ad004


title: cTEC_WT_D5


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F11_ad002


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F10_ad012


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F10_ad010


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F10_ad008


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F10_ad006


title: cTEC_WT_D22


title: cTEC_WT_D24


title: cTEC_WT_D21


title: cTEC_WT_D23


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F10_ad004


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F1_ad001


title: cTEC_WT_D2


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F1_ad007


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F10_ad001


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F1_ad010


title: cTEC_WT_D17


title: cTEC_WT_D18


title: cTEC_WT_D19


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F1_ad006


title: cTEC_WT_D4


title: cTEC_WT_D15


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F1_ad004


title: cTEC_WT_D3


title: cTEC_WT_D14


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F1_ad002


title: cTEC_WT_D16


title: cTEC_WT_D12


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F1_ad008


title: cTEC_WT_D20


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E9_ad012


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F10_ad002


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F1_ad012


title: cTEC_WT_D11


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E9_ad007


title: cTEC_WT_D10


title: cTEC_WT_C9


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E9_ad004


title: cTEC_WT_C8


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E9_ad010


title: cTEC_WT_C7


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E9_ad008


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E8_ad012


title: cTEC_WT_D13


title: cTEC_WT_C6


title: cTEC_WT_C5


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E8_ad001


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E8_ad007


title: cTEC_WT_C4


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E8_ad010


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E8_ad004


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E9_ad001


title: cTEC_WT_C24


title: cTEC_WT_C23


title: cTEC_WT_D1


title: cTEC_WT_C3


title: cTEC_WT_C22


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E8_ad008


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E8_ad002


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E9_ad006


title: cTEC_WT_C20


title: cTEC_WT_C21


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E7_ad001


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E7_ad012


title: cTEC_WT_C2


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E7_ad007


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E7_ad010


title: cTEC_WT_C19


title: cTEC_WT_C18


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E8_ad006


title: cTEC_WT_C16


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E7_ad008


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E7_ad004


title: cTEC_WT_C15


title: cTEC_WT_C14


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E7_ad002


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E6_ad012


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E6_ad001


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E6_ad007


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E6_ad010


title: cTEC_WT_C11


title: cTEC_WT_C10


title: cTEC_WT_C13


title: cTEC_WT_C1


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E6_ad006


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E6_ad008


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E6_ad004


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E6_ad002


title: cTEC_WT_B9


title: CEMBA190108_6A_1_CEMBA190108_6A_2_F5_ad001


title: cTEC_WT_C17


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E5_ad012


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E7_ad006


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E5_ad007


title: cTEC_WT_B5


title: cTEC_WT_B6


title: cTEC_WT_B3


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E5_ad006


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E5_ad004


title: cTEC_WT_C12


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E5_ad008


title: cTEC_WT_B24


title: cTEC_WT_B4


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E5_ad010


title: cTEC_WT_B23


title: cTEC_WT_B8


title: cTEC_WT_B21


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E4_ad012


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E4_ad007


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E4_ad001


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E4_ad006


title: cTEC_WT_B22


title: cTEC_WT_B7


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E4_ad004


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E4_ad002


title: cTEC_WT_B20


title: cTEC_WT_B19


title: cTEC_WT_B2


title: cTEC_WT_B18


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E3_ad001


title: cTEC_WT_B17


title: cTEC_WT_B16


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E3_ad010


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E3_ad006


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E3_ad004


title: cTEC_WT_B14


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E3_ad002


title: cTEC_WT_B13


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E3_ad008


title: cTEC_WT_B10


title: cTEC_WT_B12


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E2_ad012


title: cTEC_WT_B11


title: cTEC_WT_B1


title: cTEC_WT_A9


title: cTEC_WT_A8


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E2_ad001


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E2_ad007


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E2_ad006


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E2_ad004


title: cTEC_WT_A7


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E12_ad012


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E3_ad007


title: cTEC_WT_A5


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E2_ad002


title: cTEC_WT_A3


title: cTEC_WT_A4


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E12_ad010


title: cTEC_WT_A6


title: cTEC_WT_B15


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E12_ad007


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E12_ad008


title: cTEC_WT_A23


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E12_ad004


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E12_ad002


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E12_ad001


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E11_ad010


title: cTEC_WT_A2


title: cTEC_WT_A21


title: cTEC_WT_A22


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E11_ad008


title: cTEC_WT_A20


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E2_ad010


title: cTEC_WT_A18


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E11_ad006


title: cTEC_WT_A16


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E11_ad007


title: cTEC_WT_A15


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E11_ad004


title: cTEC_WT_A24


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E11_ad002


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E10_ad012


title: cTEC_WT_A14


title: cTEC_WT_A13


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E11_ad001


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E10_ad010


title: cTEC_WT_A12


title: cTEC_WT_A10


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E10_ad007


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E10_ad004


title: cTEC_WT_A1


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E10_ad002


title: cTEC_Eed_P9


title: cTEC_WT_A19


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E10_ad008


title: cTEC_WT_A17


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E1_ad007


title: cTEC_Eed_P6


title: cTEC_Eed_P7


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E1_ad002


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E1_ad010


title: cTEC_Eed_P5


title: cTEC_Eed_P8


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E1_ad008


title: cTEC_Eed_P4


title: cTEC_Eed_P24


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D9_ad012


title: cTEC_Eed_P3


title: cTEC_WT_A11


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D9_ad007


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D9_ad001


title: cTEC_Eed_P23


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D9_ad010


title: cTEC_Eed_P21


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D9_ad006


title: cTEC_Eed_P20


title: cTEC_Eed_P22


title: CEMBA190108_6A_1_CEMBA190108_6A_2_E10_ad001


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D9_ad002


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D9_ad008


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D8_ad012


title: cTEC_Eed_P19


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D8_ad001


title: cTEC_Eed_P17


title: cTEC_Eed_P15


title: cTEC_Eed_P16


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D8_ad007


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D8_ad006


title: cTEC_Eed_P13


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D8_ad004


title: cTEC_Eed_P12


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D8_ad010


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D8_ad008


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D8_ad002


title: cTEC_Eed_P11


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D7_ad012


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D9_ad004


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D7_ad006


title: cTEC_Eed_P10


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D7_ad004


title: cTEC_Eed_O7


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D7_ad002


title: cTEC_Eed_O8


title: cTEC_Eed_O6


title: cTEC_Eed_P2


title: cTEC_Eed_O9


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D7_ad008


title: cTEC_Eed_P18


title: cTEC_Eed_P14


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D6_ad012


title: cTEC_Eed_O5


title: Chuong537: Ac WT dorsal dermis RNA-seq

city: Los Angeles

first: Hans I-Chen

last: Harn

address: 2011 Zonal Ave. HMR304

institute: University of Southern California

data_processing:
Low quality of sequencing bases were trimmed based on the Phred+33 quality score (> 20) from both of the 5'- and 3'-ends of reads. After trimming, reads are discarded, if they are shorter than 30bp, or have one or more ambiguous base.
The mouse mm10 reference genome, and RefSeq genome annotation downloaded from the UCSC Genome Browser on 5 June, 2019 were used for RNA-Seq analysis
The alignment, quantification, normalization, and differential expression analysis were performed by STAR 2.4.1d, htseq-count 0.6.0, TMM, and edgeR 3, respectively.
Genes with count-per-million (CPM) values above 1 in at least two samples were retained, and genes with no or low expression levels were discarded.
Genome_build: UCSC mm10
Supplementary_files_format_and_content: The tab-delimited text files: 25 files with count values of 24500 genes for each sample produced by htseq-count

organism: Acomys cahirinus

source_name: dorsal dermis

extract_protocol:
TRIzol
Illumina TruSeq RNA sample Prep kit v2 / Illumina TruSeq Stranded mRNA library prep kit

value: dorsal dermis

tag: post-wound day (pwd)

tag: region

tag: quality score

tag: rna-seq read type

value: Non strand-specific single-end 75bp


title: Chuong548: Ac WT dorsal dermis RNA-seq


title: cTEC_Eed_O4

tag: strain

tag: sample type

value: single cell


title: cTEC_Eed_O3

tag: zsgreen status


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D6_ad001


title: Chuong547: Ac WT dorsal dermis RNA-seq

tag: tissue


title: cTEC_Eed_O23


title: cTEC_Eed_O24


title: cTEC_Eed_P1


title: cTEC_Eed_O21


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D7_ad001


title: Chuong543: Ac WT dorsal dermis RNA-seq

tag: genotype


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D6_ad007


title: cTEC_Eed_O20


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D6_ad010


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D6_ad006


title: cTEC_Eed_O18


title: cTEC_Eed_O19


title: Chuong533: Ac WT dorsal dermis RNA-seq


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D6_ad004


title: cTEC_Eed_O17


title: cTEC_Eed_O2


title: Chuong534: Ac WT dorsal dermis RNA-seq


title: Chuong538: Ac WT dorsal epidermis RNA-seq

source_name: dorsal epidermis

value: dorsal epidermis


title: Chuong546: Ac WT dorsal epidermis RNA-seq


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D6_ad002


title: cTEC_Eed_O16


title: cTEC_Eed_O15


title: cTEC_Eed_O14


title: Chuong545: Ac WT dorsal epidermis RNA-seq


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D6_ad008


title: cTEC_Eed_O12


title: cTEC_Eed_O13


title: cTEC_Eed_O22


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D5_ad012


title: Chuong542: Ac WT dorsal epidermis RNA-seq


title: cTEC_Eed_O11


title: cTEC_Eed_O10


title: cTEC_Eed_O1


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D5_ad001


title: cTEC_Eed_N9


title: Chuong541: Ac WT dorsal epidermis RNA-seq


title: Chuong536: Ac WT dorsal epidermis RNA-seq


title: cTEC_Eed_N8


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D5_ad007


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D5_ad010


title: cTEC_Eed_N3


title: cTEC_Eed_N6


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D5_ad006


title: cTEC_Eed_N5


title: cTEC_Eed_N7


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D5_ad004


title: cTEC_Eed_N4


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D4_ad012


title: cTEC_Eed_N24


title: cTEC_Eed_N20


title: cTEC_Eed_N19


title: cTEC_Eed_N23


title: cTEC_Eed_N2


title: cTEC_Eed_N22


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D4_ad007


title: cTEC_Eed_N21


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D4_ad010


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D4_ad001


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D4_ad006


title: cTEC_Eed_N17


title: cTEC_Eed_N16


title: cTEC_Eed_N14


title: cTEC_Eed_N15


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D4_ad002


title: cTEC_Eed_N18


title: cTEC_Eed_N13


title: cTEC_Eed_N12


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D4_ad008


title: cTEC_Eed_N11


title: cTEC_Eed_N10


title: cTEC_Eed_N1


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D3_ad007


title: cTEC_Eed_M8


title: cTEC_Eed_M9


title: cTEC_Eed_M7


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D3_ad010


title: cTEC_Eed_M6


title: cTEC_Eed_M3


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D3_ad012


title: cTEC_Eed_M23


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D3_ad004


title: cTEC_Eed_M5


title: cTEC_Eed_M4


title: cTEC_Eed_M21


title: cTEC_Eed_M20


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D3_ad002


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D2_ad008


title: cTEC_Eed_M2


title: cTEC_Eed_M22


title: cTEC_Eed_M19


title: cTEC_Eed_M17


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D2_ad006


title: cTEC_Eed_M16


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D2_ad012


title: cTEC_Eed_M15


title: cTEC_Eed_M14


title: cTEC_Eed_M12


title: cTEC_Eed_M13


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D3_ad006


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D12_ad010


title: cTEC_Eed_M10


title: cTEC_Eed_M24


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D12_ad008


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D3_ad008


title: cTEC_Eed_L9


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D12_ad007


title: cTEC_Eed_M1


title: cTEC_Eed_M18


title: cTEC_Eed_L7


title: cTEC_Eed_L4


title: cTEC_Eed_L8


title: cTEC_Eed_L6


title: cTEC_Eed_L3


title: cTEC_Eed_L24


title: cTEC_Eed_L5


title: cTEC_Eed_L22


title: cTEC_Eed_L23


title: cTEC_Eed_L21


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D12_ad012


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D12_ad001


title: cTEC_Eed_M11


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D10_ad007


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D10_ad008


title: cTEC_Eed_L18


title: cTEC_Eed_L19


title: cTEC_Eed_L2


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D10_ad012


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D12_ad006


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D12_ad004


title: cTEC_Eed_L17


title: cTEC_Eed_L15


title: cTEC_Eed_L14


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D10_ad006


title: cTEC_Eed_L16


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D12_ad002


title: cTEC_Eed_L13


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D10_ad004


title: cTEC_Eed_L1


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D10_ad002


title: cTEC_Eed_K9


title: cTEC_Eed_K7


title: cTEC_Eed_L20


title: cTEC_Eed_L10


title: cTEC_Eed_K8


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D1_ad007


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D10_ad001


title: cTEC_Eed_K6


title: cTEC_Eed_K5


title: cTEC_Eed_K4


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D1_ad010


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D1_ad004


title: cTEC_Eed_K23


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D1_ad002


title: cTEC_Eed_K3


title: cTEC_Eed_K22


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D1_ad008


title: cTEC_Eed_K2


title: cTEC_Eed_K20


title: cTEC_Eed_K21


title: CEMBA190108_6A_1_CEMBA190108_6A_2_D1_ad012


title: cTEC_Eed_K19


title: cTEC_Eed_L11


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C9_ad001


title: cTEC_Eed_K15


title: cTEC_Eed_K14


title: cTEC_Eed_K16


title: cTEC_Eed_K17


title: cTEC_Eed_K18


title: cTEC_Eed_K12


title: cTEC_Eed_K13


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C9_ad006


title: cTEC_Eed_K11


title: cTEC_Eed_K10


title: cTEC_Eed_K24


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C9_ad010


title: cTEC_Eed_J9


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C9_ad002


title: cTEC_Eed_K1


title: cTEC_Eed_J8


title: cTEC_Eed_J7


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C8_ad007


title: cTEC_Eed_J5


title: cTEC_Eed_J3


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C9_ad007


title: cTEC_Eed_J4


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C8_ad012


title: cTEC_Eed_J6


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C8_ad010


title: cTEC_Eed_J23


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C8_ad006


title: cTEC_Eed_J24


title: cTEC_Eed_J22


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C9_ad004


title: cTEC_Eed_J21


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C8_ad004


title: cTEC_Eed_J19


title: cTEC_Eed_J20


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C8_ad002


title: cTEC_Eed_J18


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C8_ad008


title: cTEC_Eed_J17


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C9_ad008


title: cTEC_Eed_J2


title: cTEC_Eed_J15


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C7_ad001


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C7_ad012


title: cTEC_Eed_J13


title: cTEC_Eed_J14


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C7_ad010


title: cTEC_Eed_J10


title: cTEC_Eed_J11


title: cTEC_Eed_I9


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C7_ad006


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C7_ad004


title: cTEC_Eed_J1


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C7_ad002


title: cTEC_Eed_I8


title: cTEC_Eed_I7


title: cTEC_Eed_I6


title: cTEC_Eed_I5


title: cTEC_Eed_I4


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C6_ad012


title: cTEC_Eed_I24


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C6_ad001


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C6_ad007


title: cTEC_Eed_I22


title: cTEC_Eed_I21


title: cTEC_Eed_J16


title: cTEC_Eed_I3


title: cTEC_Eed_I23


title: cTEC_Eed_J12


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C6_ad010


title: cTEC_Eed_I20


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C6_ad006


title: cTEC_Eed_I18


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C6_ad004


title: cTEC_Eed_I17


title: cTEC_Eed_I2


title: cTEC_Eed_I14


title: cTEC_Eed_I16


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C6_ad008


title: cTEC_Eed_I13


title: cTEC_Eed_I11


title: cTEC_Eed_I15


title: cTEC_Eed_I1


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C5_ad001


title: cTEC_Eed_H8


title: cTEC_Eed_H9


title: cTEC_Eed_H7


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C5_ad004


title: cTEC_Eed_I10


title: cTEC_Eed_I19


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C5_ad002


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C5_ad006


title: cTEC_Eed_H4


title: cTEC_Eed_H6


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C6_ad002


title: cTEC_Eed_H23


title: cTEC_Eed_H3


title: cTEC_Eed_H24


title: cTEC_Eed_H21


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C4_ad002


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C4_ad010


title: cTEC_Eed_H20


title: cTEC_Eed_H2


title: cTEC_Eed_H22


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C5_ad010


title: cTEC_Eed_I12


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C4_ad008


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C4_ad007


title: cTEC_Eed_H5


title: cTEC_Eed_H19


title: cTEC_Eed_H18


title: cTEC_Eed_H13


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C4_ad012


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C4_ad006


title: cTEC_Eed_H14


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C4_ad001


title: cTEC_Eed_H16


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C3_ad012


title: cTEC_Eed_H15


title: cTEC_Eed_L12


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C5_ad008


title: cTEC_Eed_G9


title: cTEC_Eed_G8


title: cTEC_Eed_H12


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C3_ad001


title: cTEC_Eed_G7


title: cTEC_Eed_H11


title: cTEC_Eed_G6


title: cTEC_Eed_G5


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C3_ad007


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C3_ad010


title: cTEC_Eed_H10


title: cTEC_Eed_G3


title: cTEC_Eed_G4


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C3_ad006


title: cTEC_Eed_G22


title: cTEC_Eed_H1


title: cTEC_Eed_H17


title: cTEC_Eed_G23


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C3_ad008


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C3_ad004


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C2_ad012


title: cTEC_Eed_G21


title: cTEC_Eed_G14


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C3_ad002


title: 10-hours Transmigrated neutrophils from healthy donor 6 (HD6_LTB4)

city: Atlanta

first: Diego

last: Moncada

state: Georgia

address: 201 Dowman Dr

department: Pediatrics

institute: Emory University

data_processing:
Hisat2 was used to align next-generation sequencing reads to the reference genome.
SAMtools was used to sort BAM files.
FeatureCounts from the Subread package was used to generate read counts expressed per gene.
Genome_build: GRCh38
Supplementary_files_format_and_content: *__counts.txt: Tab-delimited text files with raw gene counts for every gene and every sample.

source_name: Blood

growth_protocol: We used Alvetex (Reprocell) 200 μm-thick inert 3D scaffolds with >90% porosity (pore sizes of 36–40 μm, with interconnects of 12–14 μm). In brief, inserts were activated with 70% ethanol, coated overnight at 37ºC with rat-tail collagen I (3 mg/mL, Sigma) and seeded with H441 cells at 2.5×10^5 cells per 12-well insert. Cells were first grown in submerged cultures with DMEM/F12 supplemented with 10% heat-inactivated serum, penicillin, and streptomycin. After 2 days, cells were supplemented basally with serum-free DMEM/F12 with 10% Ultroser G (Pall Life Sciences) to establish ALI. Cultures were grown for 2 weeks at ALI and supplemented basally with fresh medium every 48 hours. For TM experiments, the ALI cultures were placed with the apical compartment exposed to RPMI, leukotriene B4 (LTB4, 100 nM) or airway supernatant (ASN) from CF patients Sputum. Then, 0.5–1 ×10^6 blood neutrophils were loaded onto the 200 μm-thick basal compartment of the Alvetex scaffold (situated upside), and allowed to migrate at 37°C at 5% CO2 through the collagen and epithelial layers into the apical compartment (situated downside, and bathed with either control medium with chemoattractant, or ASN) doi: 10.1002/JLB.5HI1117-454RR. Finally, using the in vitro model, airway neutrophils (transmigrated neutrophils) were collected after 1, 2, 4 and 6 hour of transmigration towards CFASN.

extract_protocol:
RNA was isolated from purified blood (>98% purity) and transmigrated (LTB4 or CFASN, >98% purity) neutrophils using the Nucleospin RNA isolation kit (Clontech, Takara Bio) following manufacturer protocol. RNA quality and concentration were quantified using a Bioanalyzer (Agilent Technologies, Inc.) and only samples with RIN>8.0 were used for sequencing.
Libraries were prepared using the TruSeq RNA Single Indexes Set B kit (Illumina) and AmPure beads (Beckman) and sequenced as paired end (100bp) by high seq rapid run (Illumina) with an aim of ~20 million reads per sample.

treatment_protocol: In this model, naïve blood neutrophils are migrated through a small airway epithelium grown at air-liquid interface toward CF airway fluid supernatant (CFASN, which corresponds to sputum sequentially centrifuged to remove cells and bacteria) or leukotriene B4 (LTB4, transmigration control), placed apically. Specifically, we transmigrated neutrophils in the model for 10 hours into CFASN and LTB4 followed by RNA extraction.

tag: diagnosis

value: Healthy

value: Neutrophils

tag: condition

value: 10 hours transmigration toward LTB4

tag: donor


title: cTEC_Eed_G18


title: cTEC_Eed_G19


title: cTEC_Eed_G2


title: cTEC_Eed_G16


title: cTEC_Eed_G15


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C2_ad001


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C2_ad004


title: cTEC_Eed_G10


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C2_ad010


title: 10-hours Transmigrated neutrophils from healthy donor 1 (HD1_LTB4)

tag: cell type


title: cTEC_Eed_G13


title: cTEC_Eed_G12


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C2_ad002


title: cTEC_Eed_G17


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C2_ad007


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C2_ad006


title: 10-hours Transmigrated neutrophils from healthy donor 5 (HD5_LTB4)

tag: treatment


title: 10-hours Transmigrated neutrophils from healthy donor 6 (HD6_CFASN)

value: CFASN

value: 10 hours transmigration toward CFASN


title: cTEC_Eed_G11


title: 10-hours Transmigrated neutrophils from healthy donor 3 (HD3_ LTB4)


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C12_ad012

tag: brain region


title: cTEC_Eed_G24


title: 10-hours Transmigrated neutrophils from healthy donor 2 (HD2_LTB4)


title: cTEC_Eed_G20


title: cTEC_Eed_F7


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C12_ad010

tag: Sex


title: cTEC_Eed_G1


title: 10-hours Transmigrated neutrophils from healthy donor 2 (HD2_CFASN)


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C2_ad008

tag: age


title: cTEC_Eed_F9


title: cTEC_Eed_F5


title: cTEC_Eed_F8


title: Blood neutrophils from healthy donor 6 (HD6_Blood)

value: Untreated

value: Baseline no transmigration


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C12_ad001


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C12_ad002


title: 10-hours Transmigrated neutrophils from healthy donor 3 (HD3_CFASN)


title: cTEC_Eed_F3


title: cTEC_Eed_F6


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C12_ad004


title: 10-hours Transmigrated neutrophils from healthy donor 1 (HD1_CFASN)


title: 10-hours Transmigrated neutrophils from healthy donor 5 (HD5_CFASN)


title: cTEC_Eed_F4


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C12_ad008


title: Blood neutrophils from healthy donor 5 (HD5_Blood)


title: cTEC_Eed_F24


title: Blood neutrophils from healthy donor 2 (HD2_Blood)


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C11_ad008


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C11_ad012


title: Blood neutrophils from healthy donor 1 (HD1_Blood)


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C11_ad010


title: cTEC_Eed_F22


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C11_ad007


title: cTEC_Eed_F23


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C11_ad006


title: 2-hour Transmigrated neutrophils from healthy donor 3 towards LTB4 (HD3_2hr)

data_processing:
Hisat2 was used to align next-generation sequencing reads to the reference genome.
SAMtools was used to sort BAM files.
FeatureCounts from the Subread package was used to generate read counts expressed per gene.
Genome_build: GRCh38
Supplementary_files_format_and_content: *_counts.txt: Tab-delimited text files include raw gene counts for every gene.
Supplementary_files_format_and_content: Transcriptional_Blockage_CFASN_Neutrophils.csv: Comma-separated matrix table with raw gene counts for every gene and every sample.

growth_protocol: We used Alvetex (Reprocell) 200 μm-thick inert 3D scaffolds with >90% porosity (pore sizes of 36–40 μm, with interconnects of 12–14 μm). In brief, inserts were activated with 70% ethanol, coated overnight at 37ºC with rat-tail collagen I (3 mg/mL, Sigma) and seeded with H441 cells at 2.5×10^5 cells per 12-well insert. Cells were first grown in submerged cultures with DMEM/F12 supplemented with 10% heat-inactivated serum, penicillin, and streptomycin. After 2 days, cells were supplemented basally with serum-free DMEM/F12 with 10% Ultroser G (Pall Life Sciences) to establish ALI. Cultures were grown for 2 weeks at ALI and supplemented basally with fresh medium every 48 hours. For TM experiments, the ALI cultures were placed with the apical compartment exposed to RPMI, leukotriene B4 (LTB4, 100 nM) or airway supernatant (ASN) from CF patients Sputum. Then, 0.5–1 ×10^6 blood neutrophils were loaded onto the 200 μm-thick basal compartment of the Alvetex scaffold (situated upside), and allowed to migrate at 37°C at 5% CO2 through the collagen and epithelial layers into the apical compartment (situated downside, and bathed with either control medium with chemoattractant, or ASN) doi: 10.1002/JLB.5HI1117-454RR. Finally, Using the in vitro model, airway neutrophils (transmigrated neutrophils)were collected after 1, 2, 4 and 6 hour of transmigration towards CFASN.

extract_protocol:
RNA was isolated from purified blood (>98% purity) and transmigrated (LTB4 or CFASN, >98% purity) neutrophils using the Nucleospin RNA isolation kit (Clontech, Takara Bio) following manufacturer protocol. RNA quality and concentration were quantified using a Bioanalyzer (Agilent technologies, Inc.) and only samples with RIN>8.0 were used for sequencing.
Libraries were prepared using the TruSeq RNA Single Indexes Set B kit (Illumina) and AmPure beads (Beckman) and sequenced as paired end (100bp) by high seq rapid run (Illumina) with an aim of ~20 million reads per sample.

treatment_protocol: In this model, naïve blood neutrophils are migrated through a small airway epithelium grown at air-liquid interface toward CF airway fluid supernatant (CFASN, which corresponds to sputum sequentially centrifuged to remove cells and bacteria) or leukotriene B4 (LTB4, transmigration control), placed apically. Specifically, we transmigrated neutrophils in the model for 2 hours into CFASN and then treated them with the RNA polymerase II and III-specific transcriptional blocker Alpha-amanitin.

value: 2 hours transmigration


title: 2-hour Transmigrated neutrophils from healthy donor 2 towards LTB4 (HD2_2hr)


title: cTEC_Eed_F21


title: Neutrophils from healthy donor 3 (HD3_8hr) incubated for 8 hours with (plus) Alpha-Amanitin

value: CFASN / plus alpha-amanitin

value: 8 hours incubation plus alpha amanitin


title: cTEC_Eed_F20


title: cTEC_Eed_F2


title: cTEC_Eed_F19


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C11_ad004

value: ACA,PL,ILA

value: male


title: Neutrophils from healthy donor 2 (HD2_8hr) incubated for 8 hours without (minus) Alpha-Amanitin

value: CFASN / minus alpha-amanitin

value: 8 hours incubation minus alpha amanitin


title: Neutrophils from healthy donor 3 (HD3_8hr) incubated for 8 hours without (minus) Alpha-Amanitin


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C11_ad001


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C11_ad002


title: cTEC_Eed_F18

value: negative


title: Neutrophils from healthy donor 1 (HD1_8hr) incubated for 8 hours without (minus) Alpha-Amanitin


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C10_ad008


title: 2-hours Transmigrated neutrophils from healthy donor 3 (HD3_2hr)


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C10_ad006


title: 2-hours Transmigrated neutrophils from healthy donor 2 (HD2_2hr)


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C10_ad007


title: cTEC_Eed_F16

value: single cell


title: cTEC_Eed_F15


title: cTEC_Eed_F17


title: cTEC_Eed_F14


title: 2-hours Transmigrated neutrophils from healthy donor 1 (HD1_2hr)


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C10_ad004


title: cTEC_Eed_F13


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C10_ad002


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C10_ad001


title: Calu3_NoEnrich

city: West Lafayette

first: Majid

last: Kazemian

state: Indiana

address: 201 S. University Street

institute: Purdue university

data_processing:
Sequencing reads were aligned as single end to the chimeric genome of human (hg38) and SARS-CoV2 (NC_045512.2) using STAR aligner (v2.7.7a) to get HVC junctions.
The gene expression was calculated by RSEM (v1.3.3).
Genome_build: hg38+SARS-CoV2(NC_045512.2) chimeric genome
Supplementary_files_format_and_content: *.genes.results.txt: Tab-delimited text files; RSEM genes.results files.

source_name: Calu-3 cells infected with SARS-CoV2 (USA-WA1/2020)

growth_protocol: Human adenocarcinomic lung epithelial (Calu-3) cells (ATCC, HTB-55) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, GIBCO) supplemented with 10% Fetal Bovine Serum (FBS, Corning), HEPES, non-essential amino-acids, L-glutamine and 1X Antibiotic-Antimycotic solution (Gibco). All cells were maintained at 37°C and 5% CO2.

extract_protocol:
Infected cells were lysed in TRIzol (Invitrogen) and RNA was extracted using Direct-zol RNA Miniprep kit (Zymo Research) as per manufacturer’s instructions.
The VSP pool contained ~30 oligonucleotides that span all SARS-CoV2 genes (at least one oligo per gene and nearly 1 oligo per 1kb of the genome). Given our goal to additionally enrich for potential HVC events, we used two approaches, enrichment methods 1 (5’->3’ host-virus chimeras) and enrichment method 2 (5’->3’ virus-host chimeras). First strand cDNA synthesis reaction: To capture and enrich for viral, virus-host or host-virus transcripts, we first set up a 20 µl reverse transcription (RT) reaction using 100 ng of mRNA isolated from SARS-CoV2 infected using Superscript III reverse transcriptase. We used 2 pmol T7-P5-VSP oligo pool (for enrichment 1) or 50 pmol of T7-P5-Oligo dT (for enrichment 2) as “gene-specific primer” for RT reaction. After combining all the components as per the recommended protocol (Thermo Fisher, Catalog# 18080044 and 18064014), we incubated the entire reaction mixture at 25°C for 15 minutes followed by 50°C for 30 minutes for SSIII. Next, we inactivated the reaction by heating at 70°C for 15 minutes. To remove RNA/complementary RNA to the cDNA, we added 1 µl of PureLink™ RNase A (20 mg/mL) (Invitrogen Catalog# 12091021) and 1 µl (5 units) of RNase H (abmgood catalog# E018) and incubated at 37°C for 1 hour. We then purified cDNA using 1X Mag-Bind TotalPure NGS (Omega Bio-tek, Catalog# M1378-01) as per manufacturer’s instructions and eluted the cDNA in 15 µl of sterile water. To further remove the excess single-stranded short oligos, we treated the purified RT reaction with 1 µl of Exonuclease I (NEB, Catalog# M0293L) at 37°C for 30 minutes. Next, we added excess sterile water to sample to get a total volume of 40 µl. The reaction was them purified with 1X Mag-Bind TotalPure NGS beads and eluted in 15 ul of sterile water. Second strand cDNA synthesis and In vitro Transcription: Following this, we performed second strand synthesis using the NEBNext Ultra II Non-Directional RNA second strand synthesis module as per the suggested protocol (NEB, Catalog# E6111L). The synthesized DNA was purified via 1X Mag-Bind TotalPure NGS beads and eluted in ~12 ul of sterile water. 10 ul of this was then used as an input for T7 polymerase mediated In vitro Transcription (IVT) using the NEB HiScribe T7 High Yield RNA Synthesis Kit (NEB, # E2040S). Briefly, all the components were mixed as mentioned in the kit protocol and incubated at 37°C (lid at 50°C) for 16 hours. The reaction was eluted in 20 µl of sterile water after a round of 1X Mag-Bind TotalPure NGS bead cleanup. This newly transcribed RNA was quantified using a Nanodrop and to improve the hybridization kinetics and enhance signal, 500ng of the amplified RNA was fragmented by RNA Fragmentation reagent in a total reaction volume of 10 µl as per specifications (Thermo Fisher, Catalog# AM8740). Final reverse transcription and PCR enrichment of the library: Next, to generate final enriched libraries, we performed reverse transcription of the fragmented RNA with 50 pmol of P7-N6 for enrichment (1) and 2 pmol of the P7-VSP primer pool for enrichment (2), respectively, using SSIII reverse transcriptase as per the steps mentioned above. After RT, the reaction mixture was purified using 1X Mag-Bind TotalPure NGS beads and eluted in 20 µl of sterile water. 5 µl of this RT reaction was saved for running on a Bioanalyzer and to perform a qRT-PCR validation assay. The remaining 15 µl was used to PCR amplify the library by using high-fidelity Q5 DNA Polymerase (NEB, Catalog# M0491L) for 16 cycles using Universal primer and unique indices (NEB Catalog# E7335L, E7500L) in a total of in a 50 µl reaction volume. Finally, the amplified and enriched library was purified using the 0.8X Mag-Bind TotalPure NGS beads, quantified by Bioanalyzer/Tape station and then sequenced using Illumina platform.

treatment_protocol: SARS coronavirus 2 (SARS-CoV-2), isolate USA-WA1/2020 (NR-52281) was obtained from Bei resources and was propagated in Vero E6 cells in DMEM supplemented with 2% FBS, 4.5 g/L D-glucose, 4 mM L-glutamine, 10 mM Non-Essential Amino Acids, 1 mM Sodium Pyruvate and 10 mM HEPES. Infectious titers of SARS-CoV-2 were determined using TCID50 method (Reed and Muench). Fifty thousand Calu-3 were seeded in 48 well plate and allowed to form 80% confluent monolayer. SARS-CoV-2 virus was pre-treated with porcine trypsin (10ug/ml) for 15 minutes at 37 degrees. Cells were then infected with the pre-treated virus prep at a multiplicity of infection (MOI) of 10 for 1h in culture media (final concentration of trypsin on cells was 2ug/ml). After absorption, virus inoculum was removed and replaced with fresh culture media. 48hrs post infection, cells were harvested and RNA was isolated.

tag: cell line

value: lung adenocarcinoma cells

tag: infection

value: SARS-CoV2 (USA-WA1/2020)

tag: moi

tag: hvc enrichment method


title: cTEC_Eed_F12

tag: strain


title: Calu3_Enriched_VH

value: 5'->3' virus-host chimeras


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C1_ad012


title: cTEC_Eed_F11

tag: sample type


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C1_ad010


title: cTEC_Eed_F10

tag: zsgreen status


title: cTEC_Eed_F1


title: cTEC_Eed_E9


title: CEMBA190108_6A_1_CEMBA190108_6A_2_C1_ad008


title: Len +EZH2i +AZA

city: Aarhus C

first: Theresa

last: Jakobsen

address: Høegh-Guldbergs Gade 10

department: Department of Biomedicine

country: Denmark

institute: Aarhus University

data_processing:
Sequencing data were quality filtered (Phred score 20) and adapter trimmed using Trim Galore.
Filtered and trimmed sequencing reads were mapped to the human genome (HG19) using TopHat2.
CircRNA expression levels (sequencing reads aligning across particular backsplicing junctions) were quantified based on a stringent version of the find_circ bioinformatics algorithm 33, normalized to the total number of raw reads and communicated as reads per million (RPM).
Host gene expression levels were calculated as the average linear reads corresponding to the splice donor and splice acceptor sites of each circRNA in question, normalized to the total number of raw reads and communicated as reads per million (RPM).
Genome_build: HG19
Supplementary_files_format_and_content: tab-delimited text files include Reads Per Million (RPM) and Circular-To-Linear ratio (CTL)

source_name: Multiple myeloma cell line

growth_protocol: Cells were grown in suspension at 37ºC and 5% CO2, and was maintained in RPMI-1640 GlutaMAX medium supplemented with 10% fetal bovine serum, 1% penicillin-streptomycin, 1% sodium pyruvate and 0.05 mmol β-ME.

extract_protocol:
Total RNA were isolated from the cell lines using the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen) according to the manufacturer’s protocol
One microgram total RNA was rRNA depleted using the Ribo-Zero rRNA Removal Kit (Human, Mouse, Rat) (Epicentre Biotechnologies, Madison, WI, USA) according to the manufacturer's instructions followed by a purification step using AMPure XP Beads (Beckman Coulter, Brea, CA, USA). Sequencing libraries were generated using the ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre) according to the manufacturer's instructions using 12 PCR cycles.

treatment_protocol: The Len res and pom res cells were established by continuous culture of NCI-H929 cells in the presence of low-dose lenalidomide or pomalidomide for 4–6 months 32. The pom res and len res cells were treated with different combinations of epi-drugs: the DNA methyl transferase inhibitor (DNMTi), 5-azacytidine, the histone deacetylase inhibitor (HDACi), Panobinostat, the Enhancer of zeste homolog 2 inhibitor (EZH2i), EPZ-6438, and a combination of DNMTi and EZH2i, for 48 hours.

tag: tissue

value: bone marrow

value: B lymphocyte

value: Multiple myeloma cell line

value: treated with low-dose lenalidomide for 6 months + EPZ-6438 + 5-azacytidine for 48h


title: CEMBA190108_6A_1_CEMBA190108_6A_2_B9_ad012


title: Pom +EZH2i

value: treated with low-dose pomalidomide for 6 months + EPZ-6438 for 48h