title: 2008 TIV subject ID 80 at D0 post-vaccination


title: 2008 TIV subject ID 70 at D0 post-vaccination

status: Public on Jul 10 2011

type: RNA

city: SÃO PAULO

first: Helder

middle: I.

last: Nakaya

state: SÃO PAULO

address: AVENIDA PROFESSOR LINEU PRESTES, 580, Block 17

country: Brazil

institute: Universidade de São Paulo

hyb_protocol: Hybridization was performed on Human U133 Plus 2.0 Arrays (using GeneTitan platform, Affymetrix, or individual cartridges) for 16 h at 45 oC, and 60 r.p.m. in a Hybridization Oven 640 (Affymetrix), slides were washed and stained with a Fluidics Station 450 (Affymetrix).

scan_protocol: Scanning was performed on a 7th generation GeneChip Scanner 3000, and the Affymetrix GCOS software was used to perform image analysis and generate raw intensity data.

data_processing: RMA normalization was performed using Expression Console software (Affymetrix Inc, version 1.1).

label: biotin

molecule: total RNA

organism: Homo sapiens

source_name: Peripheral blood mononuclear cells of subjects vaccinated with Influenza vaccine at day 0 (before vaccination), and days 3 and 7 post-vaccination.

label_protocol: Total RNA sample quality was evaluated by spectrophotometer to determine quantity, protein contamination and organic solvent contamination, and an Agilent 2100 Bioanalyzer was used to check for RNA degradation. Two-round in vitro transcription amplification and labeling was performed starting with 50 ng intact, total RNA per sample, following the Affymetrix protocol.

extract_protocol: Following PMBC isolation from CPT, 2 x 10^6 cells were lysed in 1 ml of TRIzol and stored at -80C (Cat# 15596-026; Invitrogen Life Technologies). After all time points were collected for a subject, the samples were thawed, and the RNA isolation proceeded according to the manufacturer’s protocol.

tag: cell type

value: PBMC

tag: subject id

tag: time point

tag: vaccine

value: TIV (Fluarix, GlaxoSmithKline Biologicals)

tag: hai titer (day 0) - a/uruguay (h3n2)

tag: hai titer (day 28) - a/uruguay (h3n2)

tag: hai titer (day 0) - a/brisbane/59/2007 (h1n1)

tag: hai titer (day 28) - a/brisbane/59/2007 (h1n1)

tag: hai titer (day 0) - b/brisbane/3/2007

tag: hai titer (day 28) - b/brisbane/3/2007


title: 2008 TIV subject ID 74 at D3 post-vaccination


title: 2008 TIV subject ID 72 at D0 post-vaccination


title: 2008 TIV subject ID 72 at D7 post-vaccination


title: 2008 TIV subject ID 74 at D0 post-vaccination


title: 2008 TIV subject ID 83 at D7 post-vaccination


title: 2008 TIV subject ID 78 at D0 post-vaccination


title: 2008 TIV subject ID 73 at D7 post-vaccination


title: 2008 TIV subject ID 73 at D0 post-vaccination


title: 2008 TIV subject ID 68 at D3 post-vaccination


title: 2008 TIV subject ID 68 at D7 post-vaccination


title: 2008 TIV subject ID 68 at D0 post-vaccination


title: 2008 TIV subject ID 65 at D0 post-vaccination


title: 2008 TIV subject ID 63 at D7 post-vaccination


title: 2008 TIV subject ID 65 at D7 post-vaccination


title: 2008 TIV subject ID 53 at D3 post-vaccination


title: 2008 TIV subject ID 46 at D7 post-vaccination


title: 2008 TIV subject ID 51 at D3 post-vaccination


title: 2008 TIV subject ID 48 at D3 post-vaccination


title: 2008 TIV subject ID 53 at D0 post-vaccination


title: 2008 TIV subject ID 53 at D7 post-vaccination


title: 2008 TIV subject ID 65 at D3 post-vaccination


title: 2008 TIV subject ID 48 at D0 post-vaccination


title: 2008 TIV subject ID 47 at D7 post-vaccination


title: 2008 TIV subject ID 51 at D0 post-vaccination


title: 2008 TIV subject ID 46 at D3 post-vaccination


title: 2008 TIV subject ID 48 at D7 post-vaccination


title: 2008 TIV subject ID 46 at D0 post-vaccination


title: 2008 TIV subject ID 47 at D3 post-vaccination


title: 2008 TIV subject ID 44 at D0 post-vaccination


title: 2008 TIV subject ID 47 at D0 post-vaccination


title: pDC from influenza vaccinee (ID 55) at Day7 post-vaccination

hyb_protocol: Hybridization to Affymetrix HT Human Genome U133A Array (Affymetrix, Inc) and washing was performed on the Affymetrix GeneChip Array Station (GCAS) automation platform.

scan_protocol: The arrays were scanned on the GeneChip HT Array Plate Scanner (Affymetrix 00-0332).

source_name: pDC of subjects vaccinated with Influenza vaccine at day 0 (before vaccination) or at day 7 post-vaccination.

label_protocol: Total RNA was amplified using the WT-Ovation Pico RNA Amplification system (NuGEN) according to the manufacturer’s instructions. Next, cDNA was labeled using the Nugen FL-Ovation cDNA Biotin Module V2 kit, following the manufacturer's protocol.

growth_protocol: Frozen PBMCs from 6 TIV and 6 LAIV vaccinees at day 0 and day 7 post-immunization (24 samples) were thaw, washed with FACS buffer (PBS with 5% FBS) and counted. First, the following antibodies were used to separate ~2 x 106 PBMCs into a “DC” fraction (cell enriched by negative selection) and a “B cell and Monocyte” fraction (cell enriched by positive selection), accordingly to manufacturer’s instructions (Miltenyi Biotech, Auburn, CA): biotin anti-CD56 (clone;B159, BD Biosciences), biotin anti-CD3 (clone;UCHT1, BD Biosciences), biotin anti-CD16 (clone;3G8, BD Biosciences), anti-CD19 MicroBeads (Miltenyi Biotech, Auburn, CA), anti-CD14 MicroBeads (Miltenyi Biotech, Auburn, CA), anti-biotin MicroBeads (Miltenyi Biotech, Auburn, CA). Cells from “DC” fraction were washed 2x with FACS buffer (PBS with 5% FBS) and stained with FITC labeled lineage cocktail 1 (clones;SK7, 3G8, SJ25C1, L27, MφP9, NCAM16.2, BD Biosciences), PE labeled anti-CD123 (clone;9F5, BD Biosciences), PerCP labeled anti-HLA-DR (clone;L243(G46-6), BD Biosciences) and APC labeled anti-CD11c (clone;S-HCL-3, BD Biosciences). Cells from “B cell and Monocyte” fraction were washed 2x with FACS buffer (PBS with 5% FBS) and stained with PerCP labeled anti-HLA-DR (clone;L243(G46-6), BD Biosciences), FITC labeled anti-CD14 (clone; MφP9, BD Biosciences) and APC labeled anti-CD19 (clone;SJ25C1, BD Biosciences). Next, myeloid dendritic cells (lin-1- HLA-DR+ CD11Chigh CD123low) and plasmacytoid dendritic cells (lin-1- HLA-DR+ CD123high CD11Clow) were sorted from “DC” fraction, and B cells (HLA-DR+ CD19+) and monocytes (HLA-DR+ CD14+) sorted from “B cell and Monocyte” fraction using FACS Aria cell sorter (BD Biosciences). Following sorting, cells (~500 to ~50,000) were pelleted and resuspended in Trizol® reagent (Invitrogen, Carlsbad, CA).

extract_protocol: RNA extraction was performed using the Trizol® (Invitrogen, Life Technologies Corporation) according to the manufacturer's instructions. Concentrations of total RNA were determined using a Nanodrop spectrophotometer or via the Ribogreen RNA quantitation kit (Molecular Probes/Invitrogen). RNA purity was determined by Bioanalyzer 2100 traces (Agilent Technologies).

value: pDC

value: FluMist,  MedImmune


title: 2008 TIV subject ID 43 at D0 post-vaccination


title: 2008 TIV subject ID 63 at D0 post-vaccination


title: 2008 TIV subject ID 42 at D7 post-vaccination


title: 2008 TIV subject ID 42 at D3 post-vaccination


title: 2008 TIV subject ID 43 at D3 post-vaccination


title: 2008 TIV subject ID 44 at D7 post-vaccination


title: 2008 TIV subject ID 43 at D7 post-vaccination


title: 2008 TIV subject ID 44 at D3 post-vaccination


title: 2008 TIV subject ID 4 at D3 post-vaccination


title: Monocytes from influenza vaccinee (ID 55) at Day7 post-vaccination

source_name: Monocytes of subjects vaccinated with Influenza vaccine at day 0 (before vaccination) or at day 7 post-vaccination.

value: Monocytes


title: 2008 TIV subject ID 42 at D0 post-vaccination


title: pDC from influenza vaccinee (ID 55) at Day0 post-vaccination


title: 2008 TIV subject ID 4 at D0 post-vaccination


title: Bcells from influenza vaccinee (ID 55) at Day0 post-vaccination

source_name: Bcells of subjects vaccinated with Influenza vaccine at day 0 (before vaccination) or at day 7 post-vaccination.

value: Bcells


title: pDC from influenza vaccinee (ID 53) at Day7 post-vaccination


title: 2008 TIV subject ID 39 at D7 post-vaccination


title: mDC from influenza vaccinee (ID 55) at Day7 post-vaccination

source_name: mDC of subjects vaccinated with Influenza vaccine at day 0 (before vaccination) or at day 7 post-vaccination.

value: mDC


title: Bcells from influenza vaccinee (ID 55) at Day7 post-vaccination


title: 2008 TIV subject ID 39 at D0 post-vaccination


title: 2008 TIV subject ID 38 at D3 post-vaccination


title: 2008 TIV subject ID 38 at D7 post-vaccination


title: mDC from influenza vaccinee (ID 55) at Day0 post-vaccination


title: Bcells from influenza vaccinee (ID 53) at Day7 post-vaccination


title: mDC from influenza vaccinee (ID 53) at Day7 post-vaccination


title: mDC from influenza vaccinee (ID 53) at Day0 post-vaccination


title: pDC from influenza vaccinee (ID 53) at Day0 post-vaccination


title: Monocytes from influenza vaccinee (ID 53) at Day0 post-vaccination


title: 2008 TIV subject ID 35 at D7 post-vaccination


title: 2008 TIV subject ID 39 at D3 post-vaccination


title: 2008 TIV subject ID 38 at D0 post-vaccination


title: 2008 TIV subject ID 32 at D7 post-vaccination


title: 2008 TIV subject ID 35 at D3 post-vaccination


title: 2008 TIV subject ID 32 at D3 post-vaccination


title: 2008 TIV subject ID 32 at D0 post-vaccination


title: 2008 TIV subject ID 35 at D0 post-vaccination


title: Bcells from influenza vaccinee (ID 53) at Day0 post-vaccination


title: Monocytes from influenza vaccinee (ID 52) at Day0 post-vaccination


title: mDC from influenza vaccinee (ID 52) at Day7 post-vaccination


title: 2008 TIV subject ID 3 at D3 post-vaccination


title: 2008 TIV subject ID 3 at D7 post-vaccination


title: 2008 TIV subject ID 29 at D3 post-vaccination


title: Bcells from influenza vaccinee (ID 52) at Day7 post-vaccination


title: mDC from influenza vaccinee (ID 52) at Day0 post-vaccination


title: pDC from influenza vaccinee (ID 52) at Day7 post-vaccination


title: 2008 TIV subject ID 29 at D7 post-vaccination


title: Monocytes from influenza vaccinee (ID 52) at Day7 post-vaccination


title: Monocytes from influenza vaccinee (ID 48) at Day7 post-vaccination


title: pDC from influenza vaccinee (ID 52) at Day0 post-vaccination


title: 2008 TIV subject ID 3 at D0 post-vaccination


title: 2008 TIV subject ID 16 at D0 post-vaccination


title: 2008 TIV subject ID 2 at D7 post-vaccination


title: pDC from influenza vaccinee (ID 48) at Day7 post-vaccination


title: Bcells from influenza vaccinee (ID 48) at Day7 post-vaccination


title: Bcells from influenza vaccinee (ID 52) at Day0 post-vaccination


title: mDC from influenza vaccinee (ID 48) at Day7 post-vaccination


title: pDC from influenza vaccinee (ID 48) at Day0 post-vaccination


title: 2008 TIV subject ID 29 at D0 post-vaccination


title: 2008 TIV subject ID 2 at D0 post-vaccination


title: 2008 TIV subject ID 16 at D3 post-vaccination


title: 2008 LAIV subject ID 82 at D7 post-vaccination

value: LAIV (FluMist,  MedImmune)

tag: hai titer (day 0) - a/south dakota/6/2007 (h1n1)

tag: hai titer (day 28) - a/south dakota/6/2007 (h1n1)

tag: hai titer (day 0) - a/uruguay/716/2007  nymc x-175c (h3n2)

tag: hai titer (day 28) - a/uruguay/716/2007  nymc x-175c (h3n2)

tag: hai titer (day 0) - b/florida 4/2006

tag: hai titer (day 28) - b/florida 4/2006


title: 2008 TIV subject ID 16 at D7 post-vaccination


title: Monocytes from influenza vaccinee (ID 48) at Day0 post-vaccination


title: mDC from influenza vaccinee (ID 48) at Day0 post-vaccination


title: Bcells from influenza vaccinee (ID 48) at Day0 post-vaccination


title: Monocytes from influenza vaccinee (ID 47) at Day7 post-vaccination


title: 2008 TIV subject ID 63 at D3 post-vaccination


title: 2008 LAIV subject ID 79 at D7 post-vaccination


title: mDC from influenza vaccinee (ID 47) at Day7 post-vaccination


title: pDC from influenza vaccinee (ID 47) at Day7 post-vaccination


title: 2008 LAIV subject ID 81 at D0 post-vaccination


title: 2008 LAIV subject ID 82 at D0 post-vaccination


title: mDC from influenza vaccinee (ID 47) at Day0 post-vaccination


title: 2008 LAIV subject ID 82 at D3 post-vaccination


title: 2008 LAIV subject ID 81 at D7 post-vaccination


title: Bcells from influenza vaccinee (ID 43) at Day0 post-vaccination


title: Monocytes from influenza vaccinee (ID 55) at Day0 post-vaccination


title: Monocytes from influenza vaccinee (ID 41) at Day7 post-vaccination


title: pDC from influenza vaccinee (ID 41) at Day7 post-vaccination


title: Bcells from influenza vaccinee (ID 43) at Day7 post-vaccination


title: 2008 LAIV subject ID 79 at D3 post-vaccination


title: 2008 LAIV subject ID 77 at D7 post-vaccination


title: 2008 LAIV subject ID 81 at D3 post-vaccination


title: Monocytes from influenza vaccinee (ID 47) at Day0 post-vaccination


title: pDC from influenza vaccinee (ID 47) at Day0 post-vaccination


title: 2008 LAIV subject ID 77 at D3 post-vaccination


title: 2008 LAIV subject ID 79 at D0 post-vaccination


title: 2008 LAIV subject ID 76 at D3 post-vaccination


title: 2008 LAIV subject ID 76 at D0 post-vaccination


title: mDC from influenza vaccinee (ID 41) at Day7 post-vaccination


title: 2008 LAIV subject ID 76 at D7 post-vaccination


title: 2008 LAIV subject ID 77 at D0 post-vaccination


title: Bcells from influenza vaccinee (ID 41) at Day7 post-vaccination


title: pDC from influenza vaccinee (ID 41) at Day0 post-vaccination


title: 2008 LAIV subject ID 66 at D7 post-vaccination


title: Monocytes from influenza vaccinee (ID 4) at Day7 post-vaccination


title: Bcells from influenza vaccinee (ID 41) at Day0 post-vaccination


title: Bcells from influenza vaccinee (ID 4) at Day7 post-vaccination


title: mDC from influenza vaccinee (ID 41) at Day0 post-vaccination


title: 2008 LAIV subject ID 66 at D3 post-vaccination


title: mDC from influenza vaccinee (ID 4) at Day0 post-vaccination


title: Monocytes from influenza vaccinee (ID 4) at Day0 post-vaccination


title: Monocytes from influenza vaccinee (ID 39) at Day7 post-vaccination


title: 2008 LAIV subject ID 61 at D3 post-vaccination


title: 2008 LAIV subject ID 61 at D0 post-vaccination


title: 2008 LAIV subject ID 71 at D0 post-vaccination


title: Monocytes from influenza vaccinee (ID 41) at Day0 post-vaccination


title: 2008 LAIV subject ID 66 at D0 post-vaccination


title: Bcells from influenza vaccinee (ID 4) at Day0 post-vaccination


title: pDC from influenza vaccinee (ID 4) at Day0 post-vaccination


title: pDC from influenza vaccinee (ID 39) at Day7 post-vaccination


title: 2008 LAIV subject ID 71 at D7 post-vaccination


title: 2008 LAIV subject ID 62 at D7 post-vaccination


title: 2008 LAIV subject ID 60 at D7 post-vaccination


title: 2008 LAIV subject ID 61 at D7 post-vaccination


title: pDC from influenza vaccinee (ID 4) at Day7 post-vaccination


title: 2008 LAIV subject ID 62 at D3 post-vaccination


title: 2008 LAIV subject ID 62 at D0 post-vaccination


title: 2008 LAIV subject ID 60 at D3 post-vaccination


title: mDC from influenza vaccinee (ID 4) at Day7 post-vaccination


title: 2008 LAIV subject ID 60 at D0 post-vaccination


title: Monocytes from influenza vaccinee (ID 53) at Day7 post-vaccination


title: 2008 LAIV subject ID 71 at D3 post-vaccination


title: mDC from influenza vaccinee (ID 39) at Day7 post-vaccination


title: Monocytes from influenza vaccinee (ID 39) at Day0 post-vaccination


title: Bcells from influenza vaccinee (ID 39) at Day7 post-vaccination


title: pDC from influenza vaccinee (ID 39) at Day0 post-vaccination


title: 2008 LAIV subject ID 59 at D0 post-vaccination


title: 2008 LAIV subject ID 59 at D3 post-vaccination


title: 2008 LAIV subject ID 56 at D3 post-vaccination


title: 2008 LAIV subject ID 56 at D7 post-vaccination


title: Bcells from influenza vaccinee (ID 39) at Day0 post-vaccination


title: 2008 LAIV subject ID 55 at D0 post-vaccination


title: mDC from influenza vaccinee (ID 39) at Day0 post-vaccination


title: Monocytes from influenza vaccinee (ID 33) at Day7 post-vaccination


title: mDC from influenza vaccinee (ID 33) at Day7 post-vaccination


title: mDC from influenza vaccinee (ID 33) at Day0 post-vaccination


title: pDC from influenza vaccinee (ID 26) at Day7 post-vaccination


title: pDC from influenza vaccinee (ID 33) at Day0 post-vaccination


title: 2008 LAIV subject ID 59 at D7 post-vaccination


title: Monocytes from influenza vaccinee (ID 33) at Day0 post-vaccination


title: 2008 LAIV subject ID 55 at D3 post-vaccination


title: pDC from influenza vaccinee (ID 33) at Day7 post-vaccination


title: 2008 LAIV subject ID 55 at D7 post-vaccination


title: 2008 LAIV subject ID 52 at D3 post-vaccination


title: Monocytes from influenza vaccinee (ID 26) at Day7 post-vaccination


title: 2008 LAIV subject ID 50 at D3 post-vaccination


title: 2008 LAIV subject ID 56 at D0 post-vaccination


title: 2008 LAIV subject ID 52 at D0 post-vaccination


title: 2008 LAIV subject ID 52 at D7 post-vaccination


title: mDC from influenza vaccinee (ID 26) at Day7 post-vaccination


title: 2008 LAIV subject ID 50 at D7 post-vaccination


title: 2008 LAIV subject ID 50 at D0 post-vaccination


title: Monocytes from influenza vaccinee (ID 25) at Day7 post-vaccination


title: Bcells from influenza vaccinee (ID 26) at Day7 post-vaccination


title: Bcells from influenza vaccinee (ID 26) at Day0 post-vaccination


title: pDC from influenza vaccinee (ID 25) at Day7 post-vaccination


title: Monocytes from influenza vaccinee (ID 26) at Day0 post-vaccination


title: 2008 LAIV subject ID 49 at D3 post-vaccination


title: pDC from influenza vaccinee (ID 26) at Day0 post-vaccination


title: Bcells from influenza vaccinee (ID 25) at Day7 post-vaccination


title: Bcells from influenza vaccinee (ID 25) at Day0 post-vaccination


title: pDC from influenza vaccinee (ID 25) at Day0 post-vaccination


title: mDC from influenza vaccinee (ID 26) at Day0 post-vaccination


title: 2008 LAIV subject ID 45 at D0 post-vaccination


title: 2008 LAIV subject ID 49 at D0 post-vaccination


title: 2008 LAIV subject ID 41 at D0 post-vaccination


title: Monocytes from influenza vaccinee (ID 25) at Day0 post-vaccination


title: 2008 LAIV subject ID 45 at D3 post-vaccination


title: 2008 LAIV subject ID 41 at D7 post-vaccination


title: 2008 LAIV subject ID 40 at D3 post-vaccination


title: 2008 LAIV subject ID 33 at D0 post-vaccination


title: 2008 LAIV subject ID 33 at D7 post-vaccination


title: 2008 LAIV subject ID 40 at D0 post-vaccination


title: 2008 LAIV subject ID 49 at D7 post-vaccination


title: 2008 LAIV subject ID 33 at D3 post-vaccination


title: 2008 LAIV subject ID 40 at D7 post-vaccination


title: 2007 TIV subject ID 39 at D3 post-vaccination

tag: hai titer (day 0) - a/ solomon islands/3/2006 (h1n1)

tag: hai titer (day 28) - a/ solomon islands/3/2006 (h1n1)

tag: hai titer (day 0) - a/wisconsin/67/2005 (h3n2)

tag: hai titer (day 28) - a/wisconsin/67/2005 (h3n2)

tag: hai titer (day 0) - b/malaysia/2506/2004

tag: hai titer (day 28) - b/malaysia/2506/2004


title: 2008 LAIV subject ID 41 at D3 post-vaccination

tag: cell type

tag: subject id

tag: time point

tag: vaccine


title: 2007 TIV subject ID 39 at D7 post-vaccination


title: 2007 TIV subject ID 39 at D0 post-vaccination


title: 2007 TIV subject ID 35 at D7 post-vaccination


title: 2008 LAIV subject ID 26 at D7 post-vaccination


title: 2008 LAIV subject ID 26 at D0 post-vaccination


title: 2008 LAIV subject ID 26 at D3 post-vaccination


title: 2007 TIV subject ID 32 at D7 post-vaccination


title: 2008 LAIV subject ID 25 at D3 post-vaccination


title: 2007 TIV subject ID 32 at D3 post-vaccination


title: 2008 LAIV subject ID 25 at D7 post-vaccination


title: 2007 TIV subject ID 29 at D3 post-vaccination


title: 2008 LAIV subject ID 24 at D0 post-vaccination


title: 2008 LAIV subject ID 24 at D3 post-vaccination


title: 2007 TIV subject ID 35 at D0 post-vaccination


title: 2008 LAIV subject ID 25 at D0 post-vaccination


title: 2007 TIV subject ID 29 at D7 post-vaccination


title: 2007 TIV subject ID 32 at D0 post-vaccination


title: 2008 LAIV subject ID 14 at D7 post-vaccination


title: 2008 LAIV subject ID 22 at D7 post-vaccination


title: 2008 LAIV subject ID 14 at D3 post-vaccination


title: 2007 TIV subject ID 23 at D7 post-vaccination


title: 2007 TIV subject ID 21 at D7 post-vaccination


title: 2007 TIV subject ID 23 at D3 post-vaccination


title: 2008 LAIV subject ID 24 at D7 post-vaccination


title: 2007 TIV subject ID 35 at D3 post-vaccination


title: 2008 LAIV subject ID 14 at D0 post-vaccination


title: 2007 TIV subject ID 21 at D3 post-vaccination


title: 2008 LAIV subject ID 22 at D3 post-vaccination


title: 2008 LAIV subject ID 22 at D0 post-vaccination


title: 2007 TIV subject ID 18 at D7 post-vaccination


title: 2007 TIV subject ID 23 at D0 post-vaccination


title: 2007 TIV subject ID 21 at D0 post-vaccination


title: 2008 LAIV subject ID 6 at D3 post-vaccination


title: 2007 TIV subject ID 18 at D3 post-vaccination


title: 2008 LAIV subject ID 6 at D7 post-vaccination


title: 2007 TIV subject ID 18 at D0 post-vaccination


title: 2007 TIV subject ID 16 at D0 post-vaccination


title: 2008 LAIV subject ID 6 at D0 post-vaccination


title: 2008 LAIV subject ID 5 at D3 post-vaccination


title: 2008 LAIV subject ID 1 at D3 post-vaccination


title: 2007 TIV subject ID 12 at D0 post-vaccination


title: 2007 TIV subject ID 16 at D3 post-vaccination


title: 2008 LAIV subject ID 5 at D7 post-vaccination


title: 2008 LAIV subject ID 1 at D0 post-vaccination


title: 2007 TIV subject ID 29 at D0 post-vaccination


title: 2008 LAIV subject ID 5 at D0 post-vaccination


title: 2007 TIV subject ID 12 at D7 post-vaccination


title: 2007 TIV subject ID 16 at D7 post-vaccination


title: 2007 TIV subject ID 12 at D3 post-vaccination


title: 2008 LAIV subject ID 1 at D7 post-vaccination


title: HC11

status: Public on Jun 09 2010

city: Bethesda

first: Sai

last: Kanaparthi

state: MD

address: 7500 Old Georgetown Rd

department: BiG

country: USA

institute: Immune Tolerance Network

description: Gene expression data from Healthy Control 11

hyb_protocol: Hybridization was performed using 5 micrograms of biotinylated target, which was incubated with the GeneChip® Human Gene 1.0 ST array (Affymetrix) at 45°C for 16–20 hours. Following hybridization, non-specifically bound material was removed by washing and detection of specifically bound target was performed using the GeneChip® Hybridization, Wash and Stain kit, and the GeneChip® Fluidics Station 450 (Affymetrix).

scan_protocol: The arrays were scanned using the GeneChip® Scanner 3000 7G (Affymetrix) and raw data was extracted from the scanned images and analyzed with the Affymetrix GeneChip Operation System (GCOS, Affymetrix).

data_processing: Microarray data background adjustment, normalization, and summarization were performed using the robust multichip average method.

source_name: Healthy Control 11

label_protocol: Biotin-labelled target for the microarray experiment was prepared using 100 ng of total RNA and cDNA was synthesized using the GeneChip® WT (Whole Transcript) Sense Target Labelling and Control Reagents kit as described by the manufacturer (Affymetrix).  The sense cDNA was then fragmented by UDG (uracil DNA glycosylase) and APE 1 (apurinic/apyrimidic endonuclease 1) and biotin-labelled with TdT (terminal deoxynucleotidyl transferase) using the GeneChip® WT Terminal labelling kit (Affymetrix, Santa Clara, USA). Hybridization was performed using 5 micrograms of biotinylated target, which was incubated with the GeneChip® Human Gene 1.0 ST array (Affymetrix) at 45°C for 16–20 hours.

extract_protocol: RNA was extracted from whole blood collected in ABI Tempus tubes.  Extraction was performed using ABI6100 instrument.

treatment_protocol: Participants in this study received no protocol related treatment.

tag: tissue

value: Blood

tag: phenotype

value: Healthy Control


title: HC10

description: Gene expression data from Healthy Control 10

source_name: Healthy Control 10


title: HC12

description: Gene expression data from Healthy Control 12

source_name: Healthy Control 12


title: HC1

description: Gene expression data from Healthy Control 1

source_name: Healthy Control 1


title: HC3

description: Gene expression data from Healthy Control 3

source_name: Healthy Control 3


title: HC6

description: Gene expression data from Healthy Control 6

source_name: Healthy Control 6


title: SI25

description: Gene expression data from Standard Immunotherapy 25

source_name: Standard Immunotherapy 25

value: Standard Immunotherapy


title: SI27

description: Gene expression data from Standard Immunotherapy 27

source_name: Standard Immunotherapy 27


title: SI24

description: Gene expression data from Standard Immunotherapy 24

source_name: Standard Immunotherapy 24


title: HC8

description: Gene expression data from Healthy Control 8

source_name: Healthy Control 8


title: HC4

description: Gene expression data from Healthy Control 4

source_name: Healthy Control 4


title: HC2

description: Gene expression data from Healthy Control 2

source_name: Healthy Control 2


title: HC9

description: Gene expression data from Healthy Control 9

source_name: Healthy Control 9


title: HC7

description: Gene expression data from Healthy Control 7

source_name: Healthy Control 7


title: SI15

description: Gene expression data from Standard Immunotherapy 15

source_name: Standard Immunotherapy 15


title: SI16

description: Gene expression data from Standard Immunotherapy 16

source_name: Standard Immunotherapy 16


title: SI14

description: Gene expression data from Standard Immunotherapy 14

source_name: Standard Immunotherapy 14


title: SI11

description: Gene expression data from Standard Immunotherapy 11

source_name: Standard Immunotherapy 11


title: SI20

description: Gene expression data from Standard Immunotherapy 20

source_name: Standard Immunotherapy 20


title: SI21

description: Gene expression data from Standard Immunotherapy 21

source_name: Standard Immunotherapy 21


title: SI22

description: Gene expression data from Standard Immunotherapy 22

source_name: Standard Immunotherapy 22


title: SI17

description: Gene expression data from Standard Immunotherapy 17

source_name: Standard Immunotherapy 17


title: SI10

description: Gene expression data from Standard Immunotherapy 10

source_name: Standard Immunotherapy 10


title: SI26

description: Gene expression data from Standard Immunotherapy 26

source_name: Standard Immunotherapy 26


title: HC5

description: Gene expression data from Healthy Control 5

source_name: Healthy Control 5


title: SI18

description: Gene expression data from Standard Immunotherapy 18

source_name: Standard Immunotherapy 18


title: SI12

description: Gene expression data from Standard Immunotherapy 12

source_name: Standard Immunotherapy 12


title: SI13

description: Gene expression data from Standard Immunotherapy 13

source_name: Standard Immunotherapy 13


title: SI7

description: Gene expression data from Standard Immunotherapy 7

source_name: Standard Immunotherapy 7


title: SI23

description: Gene expression data from Standard Immunotherapy 23

source_name: Standard Immunotherapy 23


title: SI9

description: Gene expression data from Standard Immunotherapy 9

source_name: Standard Immunotherapy 9


title: TOL19

description: Gene expression data from Tolerant 19

value: Tolerant


title: SI8

description: Gene expression data from Standard Immunotherapy 8

source_name: Standard Immunotherapy 8


title: SI19

description: Gene expression data from Standard Immunotherapy 19

source_name: Standard Immunotherapy 19


title: TOL18

description: Gene expression data from Tolerant 18


title: SI5

description: Gene expression data from Standard Immunotherapy 5

source_name: Standard Immunotherapy 5


title: SI4

description: Gene expression data from Standard Immunotherapy 4

source_name: Standard Immunotherapy 4


title: SI6

description: Gene expression data from Standard Immunotherapy 6

source_name: Standard Immunotherapy 6


title: TOL15

description: Gene expression data from Tolerant 15


title: TOL16

description: Gene expression data from Tolerant 16


title: TOL14

description: Gene expression data from Tolerant 14


title: SI2

description: Gene expression data from Standard Immunotherapy 2

source_name: Standard Immunotherapy 2


title: SI3

description: Gene expression data from Standard Immunotherapy 3

source_name: Standard Immunotherapy 3


title: TOL13

description: Gene expression data from Tolerant 13


title: TOL12

description: Gene expression data from Tolerant 12


title: SI1

description: Gene expression data from Standard Immunotherapy 1

source_name: Standard Immunotherapy 1


title: TOL11

description: Gene expression data from Tolerant 11


title: TOL7

description: Gene expression data from Tolerant 7


title: TOL10

description: Gene expression data from Tolerant 10


title: TOL6

description: Gene expression data from Tolerant 6


title: TOL9

description: Gene expression data from Tolerant 9


title: TOL2

description: Gene expression data from Tolerant 2


title: TOL3

description: Gene expression data from Tolerant 3


title: TOL4

description: Gene expression data from Tolerant 4


title: TOL17

description: Gene expression data from Tolerant 17


title: TOL8

description: Gene expression data from Tolerant 8


title: TOL1

description: Gene expression data from Tolerant 1


title: TOL5

description: Gene expression data from Tolerant 5