title: MJ5_Emx73ctx_KO_S4

city: Lund

first: Johan

last: Jakobsson

address: Sölvegatan 17

institute: Lund University

description:
processed data file:
MM10_MJ5_Emx73ctx_KO_barcodes.tsv.gz
MM10_MJ5_Emx73ctx_KO_features.tsv.gz
MM10_MJ5_Emx73ctx_KO_matrix.mtx.gz

data_processing:
Unique mapping - RNAseq (STAR aligner (2.6.0))
Quantification of reads mapping to genes - RNAseq (featureCounts from Subread (1.6.3))
Quantification of reads mapping to TEs - RNAseq (featureCounts from Subread (1.6.3))
Quantification of reads mapping to ERVKs- RNAseq (featureCounts from Subread (1.6.3))
Quantification of reads mapping to full MMERVK10C - RNAseq (featureCounts from Subread (1.6.3))
Multi mapping - RNAseq (STAR aligner (2.6.0))
Quantification of reads mapping to genes and TE subfamilies - RNAseq (TEtranscripts from TEToolkit (2.0.3))
Base calling, demultiplexing and convertion to fastq - single cell (cellranger mkfastq (3.0))
Read quantification - single cell (cellranger count (3.0))
Unique mapping - Cut and run (Bowtie2 (2.3.4.2))
Subtraction of input signal - Cut and run (BamCompare from deeptools)
Compute mean of signals for the different guide RNAs - RNAseq (invitro CRISPR NPC)
computeMatrix for plotHeatmap of full length MMERVK10C elements (deeptools) - RNAseq (invitro CRISPR NPC) and Cut and Run
Genome_build: GRCm38.p6 vM19
Supplementary_files_format_and_content: counts matrix; binary count matrix for CR020_S9 and CR021_S10

extract_protocol:
In vitro CRISPR: Cells were detached and resuspended in basic culture media (media excluding growth factors) with propidium iodide (1:1,000; BD Biosciences) and strained (70 mm filters, BD Biosciences). RFP+ cells were FACS isolated at 4°C (reanalysis showed >99% purity) and pelleted at 1,500 rpm for 5 min, snap frozen on dry ice and stored at -80°C until RNA/DNA were isolated. For all in vivo CRISPR experiments animals were sacrificed after two months and analyzed either by IHC or nuclei isolation (see details below) followed by DNA- or RNA-seq. Emx animals (RNAseq): Animals were sacrificed at 3 months of age for either immunohistochemistry IHC or RNA sequencing. Emx animals (single cell): The nuclei isolation was performed according to Sodersten et al. (2018). The tissue was thawed and dissociated in ice-cold lysis buffer ((0.32 M sucrose, 5 mM CaCl2,  3 mM MgAc, 0.1 mM Na2EDTA, 10 mM Tris-HCl, pH 8.0, 1 mM DTT) using a 1 ml tissue douncer (Wheaton). The homogenate was carefully layered on top of a sucrose cushion (1.8  M sucrose, 3  mM MgAc, 10  mM Tris-HCl, pH 8.0, and 1 1 mM DTT) before centrifugation at 30,000 ×  g  for 2 hours and 15 min. Pelleted nuclei were softened for 10 minutes in 100 ml of nuclear storage buffer (15% sucrose, 10  mM Tris-_HCl, pH 7.2, 70  mM KCl, and 2  mM MgCl2) before resuspended in 300 ml of dilution buffer (10  mM Tris-_HCl, pH 7.2, 70  mM KCl, and 2  mM MgCl2) and run through a cell strainer (70 mm). Cells were run through the FACS (FACS Aria, BD Biosciences) at 4°C with low flowrate using a 100 mm nozzle (reanalysis showed >99% purity).
RNAseq: Total RNA was isolated from frozen cell/nuclei pellets and brain tissue using the RNeasy Mini Kit (Qiagen) and used for RNA-seq and qPCR (tissue pieces were run in the tissue lyser for 2 min, 30 Hz, prior to RNA isolation). Single cell: 8,500 nuclei per sample were sorted via FACS and loaded onto 10X Genomics Single Cell 3’ Chip along with the reverse transcription mastermix following the manufacturer’s protocol for the Chromium Single Cell 3′ Library (10X Genomics, PN-120233) to generate single-cell gel beads in emulsion. cDNA amplification was done as per the guidelines from 10x Genomics and sequencing libraries were generated with unique sample indices (SI) for each sample.
RNAseq libraries were generated using Illumina TruSeq Stranded mRNA library prep kit (poly-A selection) and sequenced on a NextSeq500 (PE 2 × 150bp). Cut and run (IgG and H3K9me3) library preparation was performed using the Hyper prep kit (KAPA biosystems) and sequenced on NextSeq500 2 x 75 bp. For the single cell sequencing, libraries for samples were multiplexed and sequenced on a Novaseq using a 150-cycle kit.

treatment_protocol: In vitro CRISPR: Transduced with a lentiviral vector expressing gRNAs (LV.gRNAs) designed to target either exon 3, 4 or 13 of Trim28 or to target lacZ as well as a nuclear RFP reporter gene (H2B-RFP). Lentiviruses were produced according to Zufferey et al  and titers were 10^9 TU/ml, which was determined using qRT-PCR. Cas9 mouse NPCs where transduced at a MOI 40 and allowed to expand for 10 days prior to FACS. In vivo CRISPR Trim28fl: AAV vectors expressing Cre were injected into the forebrain of adult floxed Trim28 animals. The production of AAV5 vectors has been described in detail elsewhere (Ulusoy et al 2009)  and titers were in the order of 1013 TU/ml, which was determined by qRT-PCR using taqman primers towards the ITR . Prior to injection, the vectors were diluted in PBS; the vectors containing the guide RNAs were diluted to 30% except upon co-injection of guides 3, 4, and 13 where the vectors were diluted to 10% each. Rosa26 Cas9 knock-in mice where anesthetized by isoflurane prior to the intra-striatal injections (coordinates from bregma: AP +0.9 mm, ML +1.8 mm, DV -2.7 mm) of 1 ml virus solution (0.1 µl/15 s). The needle was kept in place for additional 2 min post injection to avoid backflow. In vivo CRISPR Cas9: We used AAV vectors to express guides 3, 4 and 13 (targeting exon 3, 4 and 13 of Trim28) and H2B-RFP under Synapsin. The production of AAV5 vectors has been described in detail elsewhere (Ulusoy et al 2009)  and titers were in the order of 10^13 TU/ml, which was determined by qRT-PCR using taqman primers towards the ITR . Prior to injection, the vectors were diluted in PBS; the vectors containing the guide RNAs were diluted to 30% except upon co-injection of guides 3, 4, and 13 where the vectors were diluted to 10% each. Rosa26 Cas9 knock-in mice where anesthetized by isoflurane prior to the intra-striatal injections (coordinates from bregma: AP +0.9 mm, ML +1.8 mm, DV -2.7 mm) of 1 ml virus solution (0.1 µl/15 s). The needle was kept in place for additional 2 min post injection to avoid backflow. In vivo CRISPR Stop Cas9: We generated AAV-vectors expressing the gRNAs and a Cre-inducible H2B-RFP reporter, as well as an AAV-vector expressing Cre under the control of the neuron-specific Synapsin  (SYN) promoter. The production of AAV5 vectors has been described in detail elsewhere (Ulusoy et al 2009)  and titers were in the order of 10^13 TU/ml, which was determined by qRT-PCR using taqman primers towards the ITR . Prior to injection, the vectors were diluted in PBS; the vectors containing the guide RNAs were diluted to 30% except upon co-injection of guides 3, 4, and 13 where the vectors were diluted to 10% each. Rosa26 Cas9 knock-in mice where anesthetized by isoflurane prior to the intra-striatal injections (coordinates from bregma: AP +0.9 mm, ML +1.8 mm, DV -2.7 mm) of 1 ml virus solution (0.1 µl/15 s). The needle was kept in place for additional 2 min post injection to avoid backflow. Cut and Run (IgG and H3K9me3): The Cut & Run were performed according to Skene et al 2018 . In brief, 200.,000 mouse NPCs were washed, permeabilized and attached to ConA-coated magnetic beads (Bang Laboratories) before incubated with the H3K9me3 (1:50, ab8898,  Abcam) antibody at 4°C overnight. Cells were washed and incubated with pA-MNase fusion protein and digestion was activated by adding CaCl2  at 0°C. The digestion was stopped after 30 min and the target chromatin released from the insoluble nuclear chromatin before extracting the DNA. Emx animals (single cell): Animals were sacrificed by cervical dislocation and brains quickly removed. The desired regions were dissected and snap frozen on dry ice and stored at -80°C.

tag: experiment

value: Emx animals

tag: condition

value: KO

tag: genotype/variation

value: Male Emx1-Cre (+/-); Trim28-fl (+/-); gtRosa(+/-) were bred with Trim28-fl (+/+) females to generate animals in which one (Emx1-Cre +/-; Trim28-fl+/-) or both (Emx1-Cre +/-; Trim28-fl +/+) Trim28 alleles had been excised, used as control and Trim28-KO, respectively.


title: XHXCA

status: Public on Feb 24 2021

type: SRA

city: Nanjing

first: linhan

last: li

address: 168 Gushan Road, Dongshan Street, Jiangning District

country: China

institute: Affiliated Jiangning Hospital of Nanjing Medical University

library_source: transcriptomic

data_processing:
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm8 whole genome using bowtie v0.12.2 with parameters -q -p 4 -e 100 -y -a -m 10 --best --strata
Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using a protocol from Chepelev et al., Nucleic Acids Research, 2009. In short, exons from all isoforms of a gene were merged to create one meta-transcript. The number of reads falling in the exons of this meta-transcript were counted and normalized by the size of the meta-transcript and by the size of the library.
Genome_build: GRCh38
Supplementary_files_format_and_content: Matrix table with raw gene FPKM values for every gene and every sample
Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every sample

molecule: total RNA

organism: Homo sapiens

source_name: cervical cancer

extract_protocol:
Cervical cancer tissues and adjacent tissues were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent (Thermo). Illumina TruSeq Stranded Total RNA with Ribo-Zero (Cat# 20020612) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols

tag: disease state

value: cervical cancer

tag: age

tag: tissue


title: Mito screen MERS 1

city: Stanford

first: Julia

last: Belk

state: California

address: 450 Serra Mall

country: USA

institute: Stanford University

description:
CRISPR sgRNAs from gDNA
mito_counts_table.tsv
mito_mers_1

library_source: genomic

data_processing:
Adapter trimming was performed using fastp
Reads were mapped to the relevant sgRNA library using hisat2 and a custom genome (fasta) file for each sgRNA library.
BAM files were converted to counts tables using R package `Rsamtools`
Counts were depth normalized and then z-scored using negative controls
Genome_build: Designed sgRNA sequences were used to create a custom fasta file used for alignments.
Supplementary_files_format_and_content: Counts table for each sgRNA

molecule: genomic DNA

organism: Chlorocebus sabaeus

source_name: VeroE6 cells

growth_protocol: VeroE6 cells expressing Cas9v2 and their respective sgRNA pool were maintained in T150 flasks in 5% FBS.

extract_protocol:
gDNA was extracted from each sample using standard commercial kits (Zymo).
Lentivirally-integrated sgRNAs were PCR amplified from each gDNA sample and sequencing adapters and indices were added.
Targeted (amplicon) sequencing

treatment_protocol: Each culture was treated with the virus indicated in the Characteristics "infection" field above to select for resistance or sensitization sgRNAs.

tag: sgrna pool

value: Mito proteins

tag: cell line

value: VeroE6-Cas9 v2

tag: infection

value: MERS WT


title: MJ5_Emx65ctx_Ctl_S3

department: Wallenberg Neuroscience Center

country: Sweden

description:
processed data file:
MM10_MJ5_Emx65ctx_Ctl_barcodes.tsv.gz
MM10_MJ5_Emx65ctx_Ctl_features.tsv.gz
MM10_MJ5_Emx65ctx_Ctl_matrix.mtx.gz

organism: Mus musculus

growth_protocol: In vitro CRISPR: NPC cultures from Rosa26-Cas9 knock-in transgenic mice (embryonic day 13.5). The forebrain was mechanically dissociated and plated in gelatin coated flasks and maintained as a monolayer culture in NSA medium (Euromed, Euroclone) supplemented with N2 hormone mix, EGF (20 ng/ml; Gibco), bFGF (20 ng/ml; Gibco), 2 nM L-glutamine and 100 ug/ml Pen/Strep. Cells were then passaged 1:3-1:6 every 2-3 days using Accutase (Gibco).

value: Control


title: DAQZC

source_name: adjacent tissue

value: adjacent tissue


title: MJ5_Emx64ctx_Ctl_S2

description:
processed data file:
MM10_MJ5_Emx64ctx_Ctl_barcodes.tsv.gz
MM10_MJ5_Emx64ctx_Ctl_features.tsv.gz
MM10_MJ5_Emx64ctx_Ctl_matrix.mtx.gz


title: 3a-control_shCHD1_replicate 1

city: Winston-Salem

first: Douglas

middle: Scott

last: Lyles

state: NC

address: Medical Center Blvd.

department: Biochemistry

institute: Wake Forest School of Medicine

data_processing:
base-calling by Illumina Real Time Analysis RTA 2.0
alignment of reads by STAR sequence aligner v2.4.0
counts extracted by FeatureCounts v1.6.1
Genome_build: hg19 and NCBI Reference Sequence: NC_001560.1 for vesicular stomatitis virus
Supplementary_files_format_and_content: tab-delimited text file of raw counts for all samples

growth_protocol: PC3 cells were cultured in RPMI medium with 10% fetal calf serum

extract_protocol:
RNA was extracted using the RNeasy kit (Qiagen) from three independent samples of each cell line.
Total RNA was used to prepare cDNA libraries using the Illumina® TruSeq Stranded Total RNA with Ribo-Zero Gold Preparation kit (Illumina Inc.)

treatment_protocol: ShControl, shMAP3K7, shCHD1, and shMAP3K7/CHD1 cell lines were generated via transduction of PC3 cells with lentiviral vectors as previously described (Rodrigez et al. Cancer Res 2015;75:1021–34). Cells were mock-infected or infected with M51R mutant vesicular stomatitis virus at a multiplicity of 10 pfu/cell for 6 hours. Mock-infected and virus-infected cells were analyzed in separate experiments.

tag: cell type

value: prostate cancer cells

tag: virus


title: DAQCA


title: Mito screen SARS2 2

description:
CRISPR sgRNAs from gDNA
mito_counts_table.tsv
mito_sars2_2


title: 2c-shMAP3K7-Control_replicate 3


title: Mito screen SARS2 1

description:
CRISPR sgRNAs from gDNA
mito_counts_table.tsv
mito_sars2_1


title: 370second

type: RNA

city: Paris

first: Pierre

last: de la Grange

address: 29, rue du Faubourg Saint-Jacques

department: Paris Biotech Santé

country: France

institute: GenoSplice technology

hyb_protocol: Samples were hybridized using Affymetrix hybridization kit materials • Heat cocktails at 99° for 5 minutes, then 42° for 5 minutes centrifuge at max speed for 1 minute (N.B. this deviates from Affy SOP). • Transfer 200μl of hyb solution to each array, then tape holes and parafilm. • Hybridize 16 hours at 45° at 60rpm• Fluidics washing is FS450_0001

scan_protocol: Affymetrix Gene ChIP Scanner 3000 7G

data_processing: Data were normalized using Expression Console from Affymetrix (RMA-GENE analysis)

label: biotin

source_name: Healthy subject

label_protocol: Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)

extract_protocol:
Neutrophils were isolated (using Ficoll, Dextran and water to lyse the red cells) from whole blood of 5 healthy subjects, 5 patients with stable cirrhosis and 5 patients with ACLF.  RNA was then extracted and amplified
RNA was extracted using chloroform, isopropanol and ethanol

value: neutrophils

tag: stage of cirrhosis

value: No

value: Healthy control


title: 2b-shMAP3K7-Control_replicate 2


title: Mito screen EMCV 1

description:
CRISPR sgRNAs from gDNA
mito_counts_table.tsv
mito_emcv_1

value: EMCV


title: Mito screen HKU5 2

description:
CRISPR sgRNAs from gDNA
mito_counts_table.tsv
mito_hku5_2


title: 2a-shMAP3K7-Control_replicate 1


title: KYSE150_shAC007128.1_3 RNA-seq

city: Jinan

first: chuanxin

last: wang

address: 247 Beiyuan Street, Jinan 250033, Shandong, China,

institute: The Second Hospital of Shandong University

data_processing:
Basecalls performed using CASAVA version 1.8
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence using FASTX-Toolkit  version 0.0.13 and Perl version 5.8.8
mRNA-seq reads were aligned to the mm10 genome assembly using Tophat version 2.0.6 with parameters: -r 100 -I 50000  --max-segment-intron 50000 -a 10 -g 10
Gene abundance measurements were generationed and normalized  using Cufflink version 2.0.2.Linux_x86_64 ; The normalized standard were Fragments Per Kilobase of exon per Megabase of library size (FPKM)
Differential expression were analysed  using DEGseq function package in R program version 2.10.0
Genome_build: GRCh38.91
Supplementary_files_format_and_content: tab-delimited text files include normalized FPKM values and raw fragment counts for each Sample

growth_protocol: The esophageal squamous cell line (ESCC)KYSE150 was transfected with shCtrl under growth permissive conditions at 37 °C in RPMI 1640 supplemented with 10% fetal bovine serum (FBS).

extract_protocol:
Total RNA was extracted with AMBION AM1560 kit.
Following "TruSeq RNA Sample Preparation Guide".

treatment_protocol: The esophageal squamous cell line (ESCC)KYSE150 was transfected with shAC007128.1 under growth permissive conditions at 37 °C in RPMI 1640 supplemented with 10% fetal bovine serum (FBS).

value: esophageal squamous cell carcinoma (ESCC)


title: KYSE150_shAC007128.1_2 RNA-seq


title: KYSE150_shNC_3 RNA-seq


title: 1c-Control-Control_replicate 3


title: Mito screen rcVSV 2

description:
CRISPR sgRNAs from gDNA
mito_counts_table.tsv
mito_rcVSV_2


title: KYSE150_shAC007128.1_1 RNA-seq


title: Mito screen HKU5 1

description:
CRISPR sgRNAs from gDNA
mito_counts_table.tsv
mito_hku5_1


title: 1b-Control-Control_replicate 2


title: 1a-Control-Control_replicate 1


title: Mito screen Mock 2

description:
CRISPR sgRNAs from gDNA
mito_counts_table.tsv
mito_mock_2


title: Mito screen Mock 1

description:
CRISPR sgRNAs from gDNA
mito_counts_table.tsv
mito_mock_1


title: 3b-control-shCHD1_replicate 2


title: Expanded Interactome IAV 1

description:
CRISPR sgRNAs from gDNA
exp_counts_table.tsv
exp_iav_1

value: SARS-CoV-2 RNA interactome

value: IAV-WSN


title: KYSE150_shNC_2 RNA-seq


title: Antibody-derived tags from RAS mutant patient #3 [mut3_ADT]

city: Aurora

first: Brett

last: Stevens

state: CO

institute: University of Colorado

description: oligo-d(T) primed antibody-derived tag reads

data_processing:
scRNA-seq and CITE-seq UMI count matrices were generated using the kallisto-bustools pipeline.
The Seurat package in R was then used to aggregate the matrices, which were further filtered to exclude cells not passing the following QC measures: fewer than 20% of UMIs derived from mitochondia, greater than 200 genes detected, and fewer than 100,000 UMIs.
CITE-seq antibody-derived UMIs and scRNA-seq UMIs were normalized using Seurat's centered log-ratio (CLR) normalization and standard log normalization, respectively.
Hashed samples (wt2 and wt3) were demultiplexed using Seurat.
Genome_build: GRCh38
Supplementary_files_format_and_content: count_matrix.tsv contains UMI counts per gene per cell (mRNA and antibody-derived tags).

molecule: polyA RNA

growth_protocol: Freshly thawed patient specimen, no culture involved.

extract_protocol:
Cells were processed using the 10X Genomics Chromium platform to generate paired single-cell RNA-Seq and CITE-seq libraries.
10X Genomics 3' end library kit (v3)

treatment_protocol: No treatment involved.

tag: subject status

value: acute myeloid leukemia (AML) patient

tag: disease stage

value: Diagnosis

tag: patient group/id

value: Bone marrow

tag: cell populations

value: All subpopulations, no enrichment


title: zVAD, sample 4

first: Christer

last: Larsson

address: Medicon Village, Bldg 404:C3

department: Laboratory Medicine

institute: Lund university

description: ZVADD

data_processing:
Demultiplexing was done with bcl2fastq v2.18.0.12
Alignment was done with HISAT2
Expression count was done with StringTie
Genome_build: GRCh38
Supplementary_files_format_and_content: Expression count

source_name: MCF7 cells

growth_protocol: MCF7 cells were grown in 1640 RPMI medium supplemented with 10% FBS, 1 mM sodium pyruvate and penicillin+streptomycin

extract_protocol:
RNeasy Mini Kit (QIAgen)
TruSeq® Stranded mRNA Library Prep (20020594, Illumina)

treatment_protocol: Cells were treated with 10 uM LCL-161, 100 ng/ml TRAIL, and/or 20 uM zVAD-fmk as indicated

tag: agent

value: zVAD


title: Expanded Interactome EMCV 2

description:
CRISPR sgRNAs from gDNA
exp_counts_table.tsv
exp_emcv_2

value: VeroE6-Cas9 v2


title: MJ5_Emx60ctx_KO_S1

description:
processed data file:
MM10_MJ5_Emx60ctx_KO_barcodes.tsv.gz
MM10_MJ5_Emx60ctx_KO_features.tsv.gz
MM10_MJ5_Emx60ctx_KO_matrix.mtx.gz

tag: experiment

value: Emx animals

value: KO

value: Male Emx1-Cre (+/-); Trim28-fl (+/-); gtRosa(+/-) were bred with Trim28-fl (+/+) females to generate animals in which one (Emx1-Cre +/-; Trim28-fl+/-) or both (Emx1-Cre +/-; Trim28-fl +/+) Trim28 alleles had been excised, used as control and Trim28-KO, respectively.


title: Expanded Interactome EMCV 1

description:
CRISPR sgRNAs from gDNA
exp_counts_table.tsv
exp_emcv_1


title: Antibody-derived tags from RAS mutant patient #2 [mut2_ADT]


title: LCL-161+TRAIL, sample 4

description: LTD


title: Expanded Interactome T1015N 2

description:
CRISPR sgRNAs from gDNA
exp_counts_table.tsv
exp_t1015n_2

value: MERS T1015N


title: Gene expression from RAS mutant patient #3 [mut3_GEX]

description: oligo-d(T) primed mRNA reads

source_name: Gene expression from RAS mutant patient


title: Gene expression from RAS mutant patient #2 [mut2_GEX]

description: oligo-d(T) primed mRNA reads


title: LCL-161+TRAIL+zVAD, sample 3

description: LTZC


title: Control, sample 4

description: ConD

value: Control


title: Expanded Interactome T1015N 1

description:
CRISPR sgRNAs from gDNA
exp_counts_table.tsv
exp_t1015n_1


title: zVAD, sample 3

description: ZVADC


title: Expanded Interactome MERS 2

description:
CRISPR sgRNAs from gDNA
exp_counts_table.tsv
exp_mers_2

value: MERS WT


title: Expanded Interactome MERS 1

description:
CRISPR sgRNAs from gDNA
exp_counts_table.tsv
exp_mers_1


title: KYSE150_shNC_1 RNA-seq


title: LCL-161+TRAIL+zVAD, sample 4

description: LTZD


title: Hashtag oligos from hashed wt patients #2 and #3 [wt2_wt3_HTO]

description: oligo-d(T) primed hashtag oligo-derived tag reads

source_name: Hashtag oligos from hashed wt patients

value: wt2 and wt3


title: Gene expression from hashed wt patients #2 and #3 [wt2_wt3_GEX]

description: oligo-d(T) primed mRNA reads

source_name: Gene expression from hashed wt patients


title: Expanded Interactome SARS2 2

description:
CRISPR sgRNAs from gDNA
exp_counts_table.tsv
exp_sars2_2


title: Antibody-derived tags from hashed wt patients #2 and #3 [wt2_wt3_ADT]


title: Expanded Interactome SARS2 1

description:
CRISPR sgRNAs from gDNA
exp_counts_table.tsv
exp_sars2_1


title: Antibody-derived tags from wt patient #1 [wt1_ADT]

source_name: Antibody-derived tags from wt patient


title: Gene expression from wt patient #1 [wt1_GEX]

description: oligo-d(T) primed mRNA reads

source_name: Gene expression from wt patient


title: LCL-161+TRAIL+zVAD, sample 2

description: LTZB


title: zVAD, sample 2

description: ZVADB


title: Expanded Interactome HKU5 2

description:
CRISPR sgRNAs from gDNA
exp_counts_table.tsv
exp_hku5_2


title: MV4;11_AS-99 3µM_3

city: Ann Arbor

first: Fengbiao

last: Mao

state: Michigan

address: 1301 Catherine St

department: Department of Pathology

institute: University of Michigan

description:
139598 AS-99_3-3
processed data file: RNA-seq.Counts.txt
processed data file: RNA-seq.FPKM.txt

data_processing:
All RNA-seq data were mapped to the hg19 genome for human using Tophat2 (2.1.1), which was shown to be accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions. Then, duplicated reads for pair-end data were removed by SAMtools (v1.5).
For RNA-seq, the bigwig files for visualization in Integrative Genomics Viewer (IGV) were generated from BAM files by using “bamCoverage” from deepTools with parameters “--ignoreDuplicates --normalizeUsingRPKM --skipNonCoveredRegions --binSize 50”.
Aligned reads were assembled using Cufflinks (v2.2.1) and assembled transcriptome catalog were used as input for Cuffdiff2 to determine expression levels (FPKM, Fragments Per Kilobase per Million mapped reads) and differential expression between conditions using default options. Genes with p<0.01 and fold change (FC) >2 were considered to be differentially expressing genes.
Genome_build: hg19 (GRCh37)
Supplementary_files_format_and_content: RNA-seq.Counts.txt: Tab-delimited text file includes normalized read counts.
Supplementary_files_format_and_content: RNA-seq.FPKM.txt: Tab-delimited text file includes FPKM values.

source_name: MV4;11 leukemia cell line

growth_protocol: MV4;11 human leukemia cells were cultured in RPMI-1640 supplemented with 10% heat inactivated FBS and 1% Penicillin/ Streptomycin.

extract_protocol:
RNA isolation and DNase treatment were according to instructions of RNeasy Mini Kit, QIAGEN.
RNA libraries were prepared with the Illumina TruSeq protocol according to the manufacturer’s protocol for RNA-seq libraries.
Paired-end readings.

treatment_protocol: Cells were treated with 3 µM AS-99 or 0.25% DMSO for 6 days.

value: MV4;11 leukemia cell line

tag: treatment


title: Expanded Interactome HKU5 1

description:
CRISPR sgRNAs from gDNA
exp_counts_table.tsv
exp_hku5_1


title: MV4;11_AS-99 3µM_2

description:
139597 AS-99_3-2
processed data file: RNA-seq.Counts.txt
processed data file: RNA-seq.FPKM.txt


title: LCL-161+TRAIL, sample 2

description: LTB


title: MV4;11_AS-99 3µM_1

description:
139596 AS-99_3-1
processed data file: RNA-seq.Counts.txt
processed data file: RNA-seq.FPKM.txt


title: LCL-161+TRAIL+zVAD, sample 1

description: LTZA


title: Expanded Interactome rcVSV 2

description:
CRISPR sgRNAs from gDNA
exp_counts_table.tsv
exp_rcVSV_2


title: Expanded Interactome rcVSV 1

description:
CRISPR sgRNAs from gDNA
exp_counts_table.tsv
exp_rcVSV_1


title: MV4;11_DMSO_1

description:
139593 DMSO_1
processed data file: RNA-seq.Counts.txt
processed data file: RNA-seq.FPKM.txt

value: DMSO


title: Expanded Interactome Mock 2

description:
CRISPR sgRNAs from gDNA
exp_counts_table.tsv
exp_mock_2

value: SARS-CoV-2 RNA interactome


title: LCL-161+TRAIL, sample 3

description: LTC


title: Expanded Interactome Mock 1

description:
CRISPR sgRNAs from gDNA
exp_counts_table.tsv
exp_mock_1


title: Control, sample 3

description: ConC


title: LCL-161+TRAIL, sample 1

description: LTA


title: zVAD, sample 1

description: ZVADA


title: MV4;11_DMSO_3

description:
139595 DMSO_3
processed data file: RNA-seq.Counts.txt
processed data file: RNA-seq.FPKM.txt


title: C. bescii 5′ RNA-Seq (TSS) glucose2

status: Public on Feb 23 2021

city: Athens

first: Michael

middle: W

last: Adams

state: Georgia

address: 220 South Jackson Street

department: Department of Biochemistry and Molecular Biology

institute: University of Georgia

data_processing: This dataset contains only a raw data set (we dona`t have processed dataset because the raw data files were not high-throughput processed, rather they were analyzed in interactive browser (Rockhopper).

organism: Caldicellulosiruptor bescii DSM 6725

source_name: cell pellet

growth_protocol: 5' TSS was analyzed for Five different growth substrates (glucose, cellobiose, crystalline cellulose (Avicel), xylose, birchwood xylan (Sigma)). Growth experiments were performed in triplicate at 78°C as closed cultures without pH control (400 mL volume, shaken at 150 rpm).   Cells were harvested in mid-to-late exponential growth stage (0.5−1.5 × 108 cells mL−1). Cells were pelleted by centrifugation at 6,000 × g for 5 minutes, supernatant was decanted, and cell pellets were frozen in liquid nitrogen.

extract_protocol:
Frozen cell pellets collected from 200 ml samples drawn from each fermenter were resuspended in 2 mls of TRIzol (Invitrogen, CA) and lysed by sonication at 40% power (9W) on a Misonix 3000 sonicator (Farmingdale, NY).  Samples were sonicated for a total of 15 seconds (3 x 5 second pulse followed by 1 minute off).  Chloroform was added, and the sample was mixed and centrifuged.  The aqueous layer was removed and mixed 1:1 with 80% ethanol.  Then the mixture was processed on a RNeasy column (Qiagen Hilden, Germany) following the manufactures protocol and the on-column DNase digestion.  RNA was eluted off the column in 35 µL RNAse free H20.  The RNA was quantified using a Nanodrop 1000 instrument (ThermoScientific, Waltham, MA) and RNA quality was accessed using a Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA) and a Nano6000 chip kit.  Purified total RNA was depleted of ribosomal RNA using Ribo-Zero rRNA Removal kit for Gram Positive Bacteria (Epicentre-Illumina, WI) following the manufactures protocol.  The depleted sample was concentrated on a RNA Clean & Concentrator-5 (Zymo Research, CA) following the manufacturer’s protocol.
5'TSS was completed on RNASeq librareis.  Depleted RNA was used as the starting material for the Epicentre ScriptSeq™ v2 RNA-Seq Library Preparation kit (Illumina compatible) utilising Epicentres Fail Safe PCR Enzyme mix (Epicentre, WI) and following the manufacturer’s protocol. cDNA, tagged with ScriptSeq adaptors (1-12), was eluted with 20 µl of Buffer EB provided in the Min-Elute PCR purification kit (Qiagen) according to the ScriptSeq™ RNA-Seq Library preparation kit protocol. Twelve PCR cycles were used during library amplification and samples were purified using Agencourt AmPureXP magnetic beads (Beckman Coulter, IN )  and eluted with 20 µl of buffer EB. The final mRNA Seq library was quantified with a Qubit Fluorometer (Invitrogen, CA) and library quality was assessed with an Agilent Bioanalyzer DNA Chip (Agilent, CA). Samples were pooled and the concentration determined.  Pools of barcoded samples were sent to Hudson Alpha for SR50 sequencing run on an Illumina Hiseq 2500 platform.
Libraries were prepared to investigate the 5’ Transcriptional Start Site (TSS).

value: 5 g/L glucose


title: C. bescii 5′ RNA-Seq (TSS) xylan2

treatment_protocol: 5' TSS was analyzed for Five different growth substrates (glucose, cellobiose, crystalline cellulose (Avicel), xylose, birchwood xylan (Sigma)). Growth experiments were performed in triplicate at 78°C as closed cultures without pH control (400 mL volume, shaken at 150 rpm).   Cells were harvested in mid-to-late exponential growth stage (0.5−1.5 × 108 cells mL−1). Cells were pelleted by centrifugation at 6,000 × g for 5 minutes, supernatant was decanted, and cell pellets were frozen in liquid nitrogen.

value: 5 g/L xylan


title: MV4;11_DMSO_2

description:
139594 DMSO_2
processed data file: RNA-seq.Counts.txt
processed data file: RNA-seq.FPKM.txt


title: Control, sample 1

description: ConA


title: C. bescii 5′ RNA-Seq (TSS) cellobiose3

value: 5 g/L cellobiose


title: C. bescii 5′ RNA-Seq (TSS) xylose2

value: 5 g/L xylose


title: C. bescii 5′ RNA-Seq (TSS) cellobiose2


title: C. bescii 5′ RNA-Seq (TSS) glucose1


title: C. bescii RNA-Seq glucose 3

data_processing:
Raw reads were first assessed for sequencing quality using SolexaQA++ tool kits.
raw reads were trimmed by using BWA dynamic trimming algorithm in the SolexaQA++ tool kits to obtain the high-quality reads (phred score > 25 and length after trimming > 25 bp).
High-quality reads were aligned to the C. bescii DSM 6725 (NC_012034) genome using the Stampy aligner software with default parameters to generate Bam files.
Raw count table was generated from the bam files using BEDTools.
Raw count table was then used for statistical analysis to identify differential gene expression using the voom method.
Supplementary_files_format_and_content: Tab-delimited text file with raw read counts for each gene and each sample.
Supplementary_files_format_and_content: Excel table with log2fold change for average Voom log2 expression values (normalized by library size) for each gene and each pair of conditions.

growth_protocol: Five different growth substrates were used (glucose, cellobiose, crystalline cellulose (Avicel), xylose, birchwood xylan (Sigma)). Growth experiments were performed in triplicate at 78°C as closed cultures without pH control (400 mL volume, shaken at 150 rpm).

extract_protocol:
Frozen cell pellets collected from 200 ml samples drawn from each fermenter were resuspended in 2 mls of TRIzol (Invitrogen, CA) and lysed by sonication at 40% power (9W) on a Misonix 3000 sonicator (Farmingdale, NY).  Samples were sonicated for a total of 15 seconds (3 x 5 second pulse followed by 1 minute off).  Chloroform was added, and the sample was mixed and centrifuged.  The aqueous layer was removed and mixed 1:1 with 80% ethanol.  Then the mixture was processed on a RNeasy column (Qiagen Hilden, Germany) following the manufactures protocol and the on-column DNase digestion.  RNA was eluted off the column in 35 µL RNAse free H20.  The RNA was quantified using a Nanodrop 1000 instrument (ThermoScientific, Waltham, MA) and RNA quality was accessed using a Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA) and a Nano6000 chip kit.  Purified total RNA was depleted of ribosomal RNA using Ribo-Zero rRNA Removal kit for Gram Positive Bacteria (Epicentre-Illumina, WI) following the manufactures protocol.  The depleted sample was concentrated on a RNA Clean & Concentrator-5 (Zymo Research, CA) following the manufacturer’s protocol.
Depleted RNA was used as the starting material for the Epicentre ScriptSeq™ v2 RNA-Seq Library Preparation kit (Illumina compatible) utilising Epicentres Fail Safe PCR Enzyme mix (Epicentre, WI) and following the manufacturer’s protocol. cDNA, tagged with ScriptSeq adaptors (1-12), was eluted with 20 µl of Buffer EB provided in the Min-Elute PCR purification kit (Qiagen) according to the ScriptSeq™ RNA-Seq Library preparation kit protocol. Twelve PCR cycles were used during library amplification and samples were purified using Agencourt AmPureXP magnetic beads (Beckman Coulter, IN )  and eluted with 20 µl of buffer EB. The final mRNA Seq library was quantified with a Qubit Fluorometer (Invitrogen, CA) and library quality was assessed with an Agilent Bioanalyzer DNA Chip (Agilent, CA). Samples were pooled and the concentration determined.  Pools of barcoded samples were sent to Hudson Alpha for SR50 sequencing run on an Illumina Hiseq 2500 platform.
Epicentre (Illumina) ScriptSeq mRNA-Seq


title: C. bescii 5′ RNA-Seq (TSS) avicel1

value: 5 g/L avicel


title: C. bescii RNA-Seq glucose 2


title: C. bescii 5′ RNA-Seq (TSS) xylose3


title: C. bescii RNA-Seq glucose 1


title: C. bescii RNA-Seq xylose 3


title: C. bescii RNA-Seq xylan 3


title: C. bescii RNA-Seq xylan 2


title: C. bescii RNA-Seq cellobiose 1


title: Control, sample 2

description: ConB


title: C. bescii RNA-Seq cellobiose 2


title: C. bescii RNA-Seq xylan 1


title: C. bescii RNA-Seq cellobiose 3


title: C. bescii 5′ RNA-Seq (TSS) xylan1


title: C. bescii RNA-Seq xylose 2


title: C. bescii RNA-Seq avicel 2


title: C. bescii RNA-Seq avicel 3


title: C. bescii RNA-Seq avicel 1


title: C. bescii RNA-Seq xylose 1


title: Donor 4-CD8+CD161- gene full

city: Houston

first: vanaja

last: konduri

state: Texas

address: One Baylor Plaza

department: Pathology&Immunology

institute: Baylor College of Medicine

description:
Donor 4-CD8+CD161-
RMA expression value derived from Transcriptome Analysis Console v3.0

hyb_protocol: Samples were hybridized with GeneChip Human WT Pico-Clariom D Exon Arrays (Affymetrix) and scanned at the Sequencing and Microarray Facility at the University of Texas MDAnderson Cancer Center.

scan_protocol: Array scanning was performed according to the manufacturer's instruction (Affymetrix)

data_processing: Raw data were processed with Transcriptome Analysis Console v3.0 (Affymetrix).  Data analysis and statistical evaluations were performed with R version 3.4.3. Class comparison paired T test was used with an FDR of 0.05% to 0.1%.

source_name: Peripheral blood PBMC

label_protocol: Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).

extract_protocol: RNA was isolated from the cells with the RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions. Inclusion of 10 mL/L of the RNase inhibitor NucleoGuard (AmpTec) in the lysis buffer improved RNA yield and recoveries of long transcripts. All samples were treated with TURBO DNA-free (Ambion) to remove trace amounts of genomic DNA.

treatment_protocol: CD8+CD161+ and CD8+CD161- T cells were magnetically sorted from healthy donor peripheral blood.

tag: cells

value: CD8+CD161- T cells

value: PDAC antigen-loaded autologous DC


title: Donor 2-CD8+CD161- gene full

description:
Donor 2-CD8+CD161-
RMA expression value derived from Transcriptome Analysis Console v3.0


title: Donor 1-CD8+CD161+ gene full

description:
Donor 1-CD8+CD161+
RMA expression value derived from Transcriptome Analysis Console v3.0

value: CD8+CD161+ T cells


title: LWX

city: Changsha

first: Kangtao

last: Wang

state: Hunan

address: No. 87 Xiangya Road, Kaifu District

department: Gastrointestinal Surgery

institute: Central South University

hyb_protocol: 0.60 ug of Cy3-labelled cRNA was fragmented at 60°C for 30 minutes in a reaction volume of 25 ul containing 1x Agilent fragmentation buffer and 2x Agilent blocking agent following the manufacturers instructions. On completion of the fragmentation reaction, 25 ul of 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to Agilent SurePrint G3 Human Gene Expression Microarray 8x60K Ver.2.0 for 17 hours at 65°C in a rotating Agilent hybridization oven. After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent) and 1 minute with 37°C GE Wash buffer 2 (Agilent), then dried immediately by brief centrifugation.

scan_protocol: Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2565CA) using one color scan setting for 8x60K array slides (Scan Area 61x21.6 mm, Scan resolution 3um, Dye channel is set to Green PMT is set to 100%).

data_processing: The scanned images were analyzed with Feature Extraction Software 10.7.1.1 (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities.

source_name: Paracancerous tissue without intravascular cancer thrombus

label_protocol: Cyanine-3 (Cy3) labeled cRNA was prepared from 0.1ug Total RNA using the Low Input Quick Amp Labeling Kit(Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA). Dye incorporation and cRNA yield were checked with the NanoDrop ND-2000 Spectrophotometer.

growth_protocol: living tissue

extract_protocol: Cells were dissoluved in TRIZOL, and total RNA were extracted with standerd protcol. RNA was further purified with Rnasy kit.

treatment_protocol: surgery

value: Paracancerous tissue of colon cancer

tag: gender

value: female


title: HZL_C

source_name: Colon cancer tissue with intravascular cancer thrombus

value: Colon cancer tissue

value: male

tag: age


title: WHF_C

source_name: Colon cancer tissue without intravascular cancer thrombus

tag: tissue


title: WCX

source_name: Paracancerous tissue with intravascular cancer thrombus


title: LJB


title: Donor 5-CD8+CD161- gene full

description:
Donor 5-CD8+CD161-
RMA expression value derived from Transcriptome Analysis Console v3.0


title: M19: hamstring tendon, patient 19

city: Auckland

first: Dorit

last: Naot

address: Private Bag 92019

department: Medicine

country: New Zealand

institute: University of Auckland

description: Gene expression data in RNA from hamstring tendon obtained from a patient under 20 years of age

hyb_protocol: cRNA samples were hybridized to GeneChip® PrimeView™ Human Gene Expression Arrays (Affymetrix). GeneChips were washed and stained in the Affymetrix Fluidics Station.

scan_protocol: The GeneChips were scanned using Affymetrix GeneChip Scanner 3000.

data_processing: The data were analyzed with Transcriptome Analysis Console 4.0.2 (Affymetrix, Inc., Thermo Fisher Scientific), using the default settings.

source_name: hamstring tendon, patient 19

label_protocol: Biotinylated, fragmented cRNA was prepared from 300 ng of total RNA using the GeneChip® 3′ IVT Express Kit (Affymetrix).

extract_protocol: Tendon tissue was ground in a mortar and pestle and homogenized using a rotor-stator homogeniser. RNA was extracted using the miRNeasy Mini-prep kit with in-column DNase digestion (Qiagen)

treatment_protocol: Hamstring tendon samples (superfluous tissue from surgeries) were stored in RNAlater for 24 hours and then in -80° until use.

value: hamstring tendon

value: under 20 y


title: Donor 5-CD8+CD161+ gene full

description:
Donor 5-CD8+CD161+
RMA expression value derived from Transcriptome Analysis Console v3.0


title: Donor 6-CD8+CD161+ gene full

description:
Donor 6-CD8+CD161+
RMA expression value derived from Transcriptome Analysis Console v3.0


title: Donor 1-CD8+CD161- gene full

description:
Donor 1-CD8+CD161-
RMA expression value derived from Transcriptome Analysis Console v3.0


title: LJB_C


title: HZL


title: Donor 2-CD8+CD161+ gene full

description:
Donor 2-CD8+CD161+
RMA expression value derived from Transcriptome Analysis Console v3.0


title: Donor 6-CD8+CD161- gene full

description:
Donor 6-CD8+CD161-
RMA expression value derived from Transcriptome Analysis Console v3.0


title: GSX_C


title: LWX_C


title: GSX


title: Donor 3-CD8+CD161- gene full

description:
Donor 3-CD8+CD161-
RMA expression value derived from Transcriptome Analysis Console v3.0


title: WHF


title: Donor 3-CD8+CD161+ gene full

description:
Donor 3-CD8+CD161+
RMA expression value derived from Transcriptome Analysis Console v3.0


title: WCX_C


title: M14: hamstring tendon, patient 14

description: Gene expression data in RNA from hamstring tendon obtained from a patient over 20 years of age

source_name: hamstring tendon, patient 14

value: over 20 y


title: Donor 4-CD8+CD161+ gene full

description:
Donor 4-CD8+CD161+
RMA expression value derived from Transcriptome Analysis Console v3.0


title: M15: hamstring tendon, patient 15

source_name: hamstring tendon, patient 15


title: M16: hamstring tendon, patient 16

source_name: hamstring tendon, patient 16


title: M18: hamstring tendon, patient 18

source_name: hamstring tendon, patient 18


title: M9: hamstring tendon, patient 9

source_name: hamstring tendon, patient 9


title: M12: hamstring tendon, patient 12

source_name: hamstring tendon, patient 12


title: M10: hamstring tendon, patient 10

source_name: hamstring tendon, patient 10


title: E14 HSC rep4

city: Beijing

first: Di

last: Liu

address: No. 45 West Street, Beijing

institute: Xuanwu Hospital, Capital Medical University

data_processing:
aligned to the mouse reference genome (mm10, downloaded from  the UCSC genome browser) using Bismark tools with default parameters
Genome_build: mm10
Supplementary_files_format_and_content: bismark cov data

source_name: E14 HSC

extract_protocol:
C57BL/6 background mice were breed in Specific Pathogen Free (SPF) condition and mice experiments were approved by the ethics committee of the Laboratory Animal Center of Academy of Military Medical Sciences. E10.0 and E11.0 Embryos were staged by somite pair (sp) counting according to 31–35 sp and 41–45 sp separately.
bisulfite conversion was performed on cell lysates using MethylCode Bisulphite Kit (Invitrogen), after bisulphite conversion, the converted templates were conducted by the standard protocol. The final quality-insured libraries were quantified using Qubit ds DNA high sensitivity dye (Invitrogen) and then sequenced on NOVA PE 150 sequencer.

value: Fetal liver

tag: developmental stage

tag: strain


title: Adult BM HSC rep2

source_name: Adult BM HSC

value: Bone Marrow

value: Adult


title: Adult BM HSC rep1


title: E14 HSC rep2


title: E14 HSC rep1


title: E14 HSC rep3


title: T1_preHSC rep1

value: AGM


title: T2_preHSC rep3


title: T1_preHSC rep4


title: T1_preHSC rep3


title: T2_preHSC rep1


title: T1_preHSC rep2


title: M17: hamstring tendon, patient 17

source_name: hamstring tendon, patient 17


title: T2_preHSC rep2


title: hemogenic endothelial cell rep3

source_name: hemogenic endothelial cell


title: hemogenic endothelial cell rep2


title: M7: hamstring tendon, patient 7

source_name: hamstring tendon, patient 7


title: M6: hamstring tendon, patient 6

source_name: hamstring tendon, patient 6


title: M2: hamstring tendon, patient 2

source_name: hamstring tendon, patient 2


title: M8: hamstring tendon, patient 8

source_name: hamstring tendon, patient 8


title: M4: hamstring tendon, patient 4

source_name: hamstring tendon, patient 4


title: M13: hamstring tendon, patient 13

source_name: hamstring tendon, patient 13


title: M1: hamstring tendon, patient 1

source_name: hamstring tendon, patient 1


title: M3: hamstring tendon, patient 3

source_name: hamstring tendon, patient 3


title: M11: hamstring tendon, patient 11

source_name: hamstring tendon, patient 11


title: M5: hamstring tendon, patient 5

source_name: hamstring tendon, patient 5


title: hemogenic endothelial cell rep1


title: arterial endothelial cell rep2

source_name: arterial endothelial cell


title: arterial endothelial cell rep1


title: WT Rep 2

city: Chapel Hill

first: Zachary

last: Carico

address: 250 Bell Tower Dr, CB7100

department: Integrative Biological and Genome Sciences program

institute: University of North Carolina

data_processing:
Reads were aligned and gene counts determined using STAR (v2.5).
Genome_build: mm10
Supplementary_files_format_and_content: Raw feature counts in tab-delimited table.

source_name: Mouse embryonic stem cells v6.5

growth_protocol: V6.5 murine embryonic stem cells (mESCs) (male) were grown in media supplemented with FBS and LIF under standard conditions, as previously described (Dowen et al, 2014)

extract_protocol:
Cells were resuspended in 1ml Trizol (Invitrogen, 15596018). Chloroform (Sigma Aldrich, C2432) was added for phase separation. RNA was purified using the Zymo RNA Clean and Concentrator Kit (Zymo, R1013)
Libraries were commercially prepared by Novogene (Sacramento, CA) using in-house library prep protocols.

tag: genotype

value: Wild Type

value: mESC v6.5


title: R586W clone 2 rep2

tag: cell type


title: R586W clone 2 rep1


title: WT Rep 1


title: R586W clone 1

data_processing:
Initial data processing, including merging of replicates, alignment, filtering, and generation of Hi-C contact maps, was perfomed using the Juicer wrapper from the Lieberman-Aiden lab.
Contact domains were called from merged, Knight-Ruiz balanced contact maps at 10kb resolution using the Arrowhead algorithm with default parameters.
Loops were called from merged, Knight-Ruiz balanced contact maps at 5kb, 10kb, and 25kb resolution using the HiCCUPs algorithm with default parameters except: -p 8,4,2 -i 14,10,6
Genome_build: mm10
Supplementary_files_format_and_content: Hi-C matrices are provided in .hic format. Filtered Hi-C contacts are provided in .txt format. Loop and domain calls are provided in .bedpe format.

growth_protocol: V6.5 murine embryonic stem cells (mESCs) (male) were grown under standard conditions, as previously described (Dowen et al, 2014)

extract_protocol:
Hi-C libraries were prepared using the Arima Hi-C kit (Arima Genomics) according to manufacturer's specifications, with one modification. Treatment with DNA ligase was performed for one hour instead of the recommended 15 minutes.
Dual-indexed Illumina sequencing libraries were prepared using Kapa Hyper Prep (Kapa Biosciences, cat.#KK8502) according to specifications provided by Arima Genomics, with one modification. Ligation of sequencing adapters was allowed to proceed for 1 hour instead of the recommended 15 minutes.

treatment_protocol: ESCs growing in exponential phase were placed in single cell suspension in 1x PBS with 1% formaldehyde. After 2 minutes, crosslinking was quenched with addition of glycine to 150mM


title: WT Rep 3


title: R586W clone 1 rep2


title: R586W clone 1 rep3


title: H3K27me3 R586W clone 2 rep 1

data_processing:
Alignment:  reads were aligned to a hybrid mm10+hg38 genome with  bowtie v 1.2.2 with parameters -v 2 -p 24 -S -m 1 --best --strata
PCR Duplicate Removal: duplicates removed with samtools v 1.9 fixmate -m followed by markdup -r -s
Peak Calling: peaks were called using MACS (v 2016-02-15) with parameters -f BED -g mm -q 0.01
Coverage File Generation: bigwig files were generated using bedtools (v 2.26) genomecov with parameters -bga -g mm10.chrom.sizes followed by ucsctools (v 320) bedGraphToBigWig
Genome_build: mm10
Supplementary_files_format_and_content: For each ChIPped factor, a Z-score-normalized BigWig coverage track of merged replicates is provided for viewing in UCSC genome browser.

extract_protocol:
Cells were crosslinked in 1% formaldehyde for 5 minutes, and lysed using sequential treatment with hypotonic and detergent-containing buffers. Chromatin was sheared to 200-1000bp using a Covaris sonicator.
Kapa Hyper Prep library kit was used according to manufacturer's instructions.

treatment_protocol: mESCs were crosslinked in 1% formaldehyde (ThermoFisher, 28906) for 5 minutes in PBS and quenched by adding glycine. Cells were lysed using Lysis Buffers 1 and 2, and recovered nuclei resuspended in 1ml Covaris Sonication Buffer (50mM Tris pH 7.5, 10mM EDTA, 0.1% SDS). Nuclei were sheared for 12 minutes using a Covaris E220 water bath sonicator with device settings of duty factor 5, PIP/W of 140, and 200 cycles per burst. Insoluble material was cleared by spinning sonicated lysates for 10 minutes at 21,000rcf. 10µg  antibody  was incubated for 8 hours with 30µl of Dynabeads in PBS, after which excess antibodies were washed from the beads. 10^7 cell equivalents of sheared chromatin were incubated overnight with the antibody-bound beads in 1 ml Sonication Buffer 1, washed with Wash Buffers 1, 2, and 3, and crosslinks were reversed overnight. DNA was purified using a ChIP DNA Clean and Concentrator Kit (Zymo, D5201) according to manufacturer’s instructions. Sequencing libraries were prepared using a Hyper Prep kit (Kapa Biosciences, KK8502) according to manufacturer’s instructions, and 50bp single-end sequencing reads were collected using the Illumina NovaSeq sequencing platform and reagents.

value: SMC1A-R586W clone 2

tag: antibody

value: abcam ab6002


title: H3K27me3 WT rep 1


title: WT rep1


title: H3K27me3 R586W clone 1 rep 1

value: SMC1A-R586W clone 1


title: H3K27me3 R586W clone 2 rep 2


title: H3K27me3 R586W clone 1 rep 2


title: H3K27ac R586W clone 2 rep 2

value: Active Motif 39135


title: H3K27ac R586W clone 1 rep 1


title: H3K27ac R586W clone 2 rep 1


title: R586W clone 2 rep3


title: H3K27ac WT rep 2


title: H3K27ac WT rep 1


title: H3K27ac R586W clone 1 rep 2


title: RAD21 R586W clone 2 rep 2

value: Bethyl A300-080A


title: RAD21 R586W clone 1 rep 1


title: RAD21 R586W clone 1 rep 2


title: RAD21 WT rep 1


title: RAD21 WT rep 2


title: R586W clone 1 rep1


title: PIT_P5-PIT_P6-B7-AD010

city: La Jolla

first: Joseph

middle: R

last: Ecker

state: CA

address: 10010 North Torrey Pines Road

department: Genomic Analysis Laboratory

institute: HHMI-Salk-Institute

data_processing:
Library strategy: snmC-seq2
Reads trimed with Cutadapt for adapters and low quality bases (Phred score >= 20)
The resulting files are mapped to in-silico bisulfite converted mm10 reference genome using Bismark.
The raw BAM file will be filtered using samtools to filter reads by MAPQ > 10 and use Picard to remove PCR duplicates
Tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls will be generated with in-house developing package using the filtered BAM files
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls. Columns are: 1. Chromosome, 2. Position, 3. Strand, 4. Cytosin Context, 5. methylated base call, 6. total mapped base call, 7. not used, place holder column

source_name: C57BL/6J Male P56 Mouse Brain

growth_protocol: All mice are C57BL/6 Male, brain dissected in P56

extract_protocol:
The snmC-seq2 protocol was applied to individual nuclei isolated from pituitary pooled from 30 adult male C57BL/6 mice
Library prepared following protocol discribed in Luo, Chongyuan, Angeline Rivkin, Jingtian Zhou, Justin P. Sandoval, Laurie Kurihara, Jacinta Lucero, Rosa Castanon, et al. 2018. “Robust Single-Cell DNA Methylome Profiling with snmC-seq2.” Nature Communications 9 (1): 3824.

value: Pituitary

tag: Sex


title: PIT_P1-PIT_P2-D4-AD007


title: PIT_P1-PIT_P2-F4-AD012


title: PIT_P7-PIT_P8-A7-AD008


title: PIT_P1-PIT_P2-H11-AD008


title: PIT_P3-PIT_P4-B4-AD002


title: PIT_P5-PIT_P6-E6-AD008


title: PIT_P7-PIT_P8-B8-AD010


title: PIT_P7-PIT_P8-H9-AD012


title: PIT_P5-PIT_P6-D9-AD012


title: PIT_P1-PIT_P2-D9-AD002


title: PIT_P1-PIT_P2-A3-AD006


title: PIT_P7-PIT_P8-A11-AD006


title: PIT_P5-PIT_P6-D8-AD008


title: PIT_P1-PIT_P2-A9-AD002


title: PIT_P7-PIT_P8-A5-AD010


title: PIT_P7-PIT_P8-D6-AD006


title: PIT_P1-PIT_P2-H4-AD012


title: PIT_P1-PIT_P2-A6-AD006


title: PIT_P5-PIT_P6-D12-AD006


title: PIT_P5-PIT_P6-B8-AD002


title: PIT_P3-PIT_P4-F6-AD001


title: PIT_P1-PIT_P2-F3-AD007


title: RAD21 R586W clone 2 rep 1


title: PIT_P1-PIT_P2-G4-AD001


title: PIT_P1-PIT_P2-E9-AD008


title: PIT_P1-PIT_P2-D7-AD007


title: PIT_P1-PIT_P2-C7-AD012


title: PIT_P3-PIT_P4-D10-AD004


title: PIT_P3-PIT_P4-F8-AD001


title: PIT_P5-PIT_P6-A8-AD012


title: PIT_P3-PIT_P4-D11-AD008


title: PIT_P5-PIT_P6-E11-AD004


title: PIT_P3-PIT_P4-C10-AD008


title: PIT_P1-PIT_P2-A9-AD006


title: PIT_P1-PIT_P2-E12-AD001


title: PIT_P7-PIT_P8-H10-AD012


title: PIT_P1-PIT_P2-F4-AD010


title: PIT_P3-PIT_P4-C8-AD012


title: PIT_P3-PIT_P4-D7-AD006


title: PIT_P7-PIT_P8-C5-AD002


title: PIT_P3-PIT_P4-D4-AD001


title: PIT_P1-PIT_P2-H6-AD008


title: PIT_P1-PIT_P2-G10-AD010


title: PIT_P1-PIT_P2-F6-AD006


title: PIT_P5-PIT_P6-D11-AD007


title: PIT_P3-PIT_P4-A12-AD008


title: PIT_P5-PIT_P6-A7-AD010


title: PIT_P1-PIT_P2-E2-AD002


title: PIT_P1-PIT_P2-A2-AD002


title: PIT_P7-PIT_P8-D6-AD007


title: PIT_P3-PIT_P4-B5-AD006


title: PIT_P3-PIT_P4-F8-AD008


title: PIT_P3-PIT_P4-E2-AD001


title: PIT_P5-PIT_P6-D12-AD007


title: PIT_P5-PIT_P6-G12-AD006


title: PIT_P3-PIT_P4-H1-AD012


title: PIT_P7-PIT_P8-F6-AD010


title: PIT_P7-PIT_P8-D12-AD012


title: PIT_P7-PIT_P8-E4-AD008


title: PIT_P1-PIT_P2-C11-AD001


title: PIT_P3-PIT_P4-D7-AD002


title: PIT_P3-PIT_P4-C2-AD002


title: PIT_P5-PIT_P6-B11-AD010


title: PIT_P3-PIT_P4-G9-AD010


title: PIT_P7-PIT_P8-B5-AD006


title: PIT_P5-PIT_P6-C11-AD012


title: PIT_P7-PIT_P8-B7-AD004


title: PIT_P7-PIT_P8-D11-AD008


title: PIT_P7-PIT_P8-G4-AD002


title: PIT_P1-PIT_P2-G10-AD007


title: PIT_P5-PIT_P6-C4-AD004


title: PIT_P3-PIT_P4-C8-AD004


title: PIT_P3-PIT_P4-D1-AD007


title: PIT_P3-PIT_P4-F9-AD010


title: PIT_P1-PIT_P2-G1-AD008


title: PIT_P5-PIT_P6-B2-AD007


title: PIT_P1-PIT_P2-G11-AD008


title: PIT_P1-PIT_P2-H5-AD006


title: PIT_P3-PIT_P4-C8-AD010


title: PIT_P5-PIT_P6-A10-AD002


title: PIT_P1-PIT_P2-D6-AD004


title: PIT_P3-PIT_P4-F3-AD007


title: PIT_P3-PIT_P4-A1-AD001


title: PIT_P1-PIT_P2-E1-AD008


title: PIT_P3-PIT_P4-B9-AD006


title: PIT_P1-PIT_P2-F7-AD008


title: PIT_P7-PIT_P8-H7-AD008


title: PIT_P5-PIT_P6-G2-AD001


title: PIT_P3-PIT_P4-D6-AD004


title: PIT_P3-PIT_P4-A5-AD010


title: PIT_P7-PIT_P8-A1-AD006


title: PIT_P1-PIT_P2-D2-AD002


title: PIT_P1-PIT_P2-D1-AD001


title: PIT_P3-PIT_P4-H12-AD010


title: PIT_P7-PIT_P8-H12-AD004


title: PIT_P1-PIT_P2-G9-AD008


title: PIT_P1-PIT_P2-D3-AD008


title: PIT_P7-PIT_P8-F5-AD002


title: PIT_P3-PIT_P4-G4-AD008


title: PIT_P3-PIT_P4-A8-AD007


title: PIT_P3-PIT_P4-D4-AD002


title: PIT_P5-PIT_P6-A8-AD010


title: PIT_P3-PIT_P4-B10-AD012


title: PIT_P5-PIT_P6-F5-AD012


title: PIT_P1-PIT_P2-H1-AD012


title: PIT_P5-PIT_P6-A4-AD004


title: PIT_P7-PIT_P8-E6-AD008


title: PIT_P1-PIT_P2-H8-AD008


title: PIT_P3-PIT_P4-C11-AD007


title: PIT_P1-PIT_P2-H12-AD007


title: PIT_P3-PIT_P4-A7-AD001


title: PIT_P5-PIT_P6-A5-AD002


title: PIT_P7-PIT_P8-D5-AD004


title: PIT_P1-PIT_P2-F2-AD008


title: PIT_P7-PIT_P8-G11-AD008


title: PIT_P5-PIT_P6-H12-AD012


title: PIT_P3-PIT_P4-E8-AD007


title: PIT_P1-PIT_P2-B4-AD012


title: PIT_P1-PIT_P2-H3-AD007


title: PIT_P1-PIT_P2-E2-AD004


title: PIT_P1-PIT_P2-B6-AD008


title: PIT_P1-PIT_P2-C3-AD007


title: PIT_P7-PIT_P8-B10-AD004


title: PIT_P7-PIT_P8-H6-AD010


title: PIT_P3-PIT_P4-B3-AD002


title: PIT_P7-PIT_P8-C6-AD004


title: PIT_P3-PIT_P4-E1-AD001


title: PIT_P3-PIT_P4-D2-AD002


title: PIT_P7-PIT_P8-F2-AD012


title: PIT_P5-PIT_P6-B1-AD007


title: PIT_P3-PIT_P4-G9-AD006


title: PIT_P5-PIT_P6-A7-AD012


title: PIT_P3-PIT_P4-A12-AD001


title: PIT_P5-PIT_P6-A8-AD006


title: PIT_P1-PIT_P2-A4-AD001


title: PIT_P7-PIT_P8-B3-AD006


title: PIT_P5-PIT_P6-E8-AD008


title: PIT_P5-PIT_P6-A12-AD006


title: PIT_P5-PIT_P6-E1-AD010


title: PIT_P5-PIT_P6-C7-AD008


title: PIT_P7-PIT_P8-G4-AD010


title: PIT_P1-PIT_P2-F1-AD002


title: PIT_P5-PIT_P6-B6-AD006


title: PIT_P3-PIT_P4-A1-AD008


title: PIT_P1-PIT_P2-A10-AD010


title: PIT_P5-PIT_P6-B9-AD007


title: PIT_P7-PIT_P8-H3-AD004


title: PIT_P3-PIT_P4-D10-AD010


title: PIT_P5-PIT_P6-B10-AD006


title: PIT_P7-PIT_P8-G9-AD006


title: PIT_P7-PIT_P8-G2-AD008


title: PIT_P1-PIT_P2-C1-AD004


title: PIT_P7-PIT_P8-G5-AD002


title: PIT_P3-PIT_P4-A10-AD006


title: PIT_P7-PIT_P8-B11-AD010


title: PIT_P5-PIT_P6-A7-AD006


title: PIT_P3-PIT_P4-B2-AD004


title: PIT_P3-PIT_P4-A11-AD004


title: PIT_P5-PIT_P6-A5-AD008


title: PIT_P7-PIT_P8-E2-AD004


title: PIT_P1-PIT_P2-H4-AD004


title: PIT_P5-PIT_P6-E3-AD008


title: PIT_P3-PIT_P4-E9-AD004


title: PIT_P7-PIT_P8-A2-AD012


title: PIT_P3-PIT_P4-B1-AD004


title: PIT_P7-PIT_P8-D9-AD008


title: PIT_P3-PIT_P4-H6-AD001


title: PIT_P3-PIT_P4-F4-AD004


title: PIT_P1-PIT_P2-E10-AD008


title: PIT_P1-PIT_P2-D8-AD002


title: PIT_P5-PIT_P6-H8-AD010


title: PIT_P1-PIT_P2-C8-AD002


title: PIT_P3-PIT_P4-G2-AD006


title: PIT_P7-PIT_P8-D9-AD006


title: PIT_P5-PIT_P6-A4-AD010


title: PIT_P5-PIT_P6-B8-AD010


title: PIT_P1-PIT_P2-B2-AD007


title: PIT_P3-PIT_P4-G11-AD010


title: PIT_P5-PIT_P6-B6-AD001


title: PIT_P5-PIT_P6-G9-AD006


title: PIT_P7-PIT_P8-E5-AD007


title: PIT_P3-PIT_P4-E7-AD010


title: PIT_P1-PIT_P2-B4-AD007


title: PIT_P7-PIT_P8-G1-AD010


title: PIT_P1-PIT_P2-D7-AD001


title: PIT_P3-PIT_P4-E1-AD008


title: PIT_P5-PIT_P6-D7-AD010


title: PIT_P1-PIT_P2-G10-AD004


title: PIT_P1-PIT_P2-A12-AD004


title: PIT_P3-PIT_P4-D2-AD007


title: PIT_P5-PIT_P6-G3-AD010


title: PIT_P1-PIT_P2-E10-AD002


title: PIT_P7-PIT_P8-A6-AD001


title: PIT_P7-PIT_P8-D6-AD004


title: PIT_P5-PIT_P6-G6-AD010


title: PIT_P1-PIT_P2-D3-AD004


title: PIT_P7-PIT_P8-D7-AD002


title: PIT_P1-PIT_P2-G4-AD004


title: PIT_P5-PIT_P6-C8-AD006


title: PIT_P1-PIT_P2-F12-AD008


title: PIT_P3-PIT_P4-D4-AD008


title: PIT_P1-PIT_P2-D3-AD006


title: PIT_P7-PIT_P8-C7-AD004


title: PIT_P3-PIT_P4-C8-AD007


title: PIT_P1-PIT_P2-H7-AD012


title: PIT_P3-PIT_P4-G6-AD007


title: PIT_P3-PIT_P4-A1-AD007


title: PIT_P5-PIT_P6-B6-AD002


title: PIT_P1-PIT_P2-G4-AD002


title: PIT_P1-PIT_P2-D8-AD012


title: PIT_P7-PIT_P8-A11-AD001


title: PIT_P7-PIT_P8-G12-AD004


title: PIT_P5-PIT_P6-G10-AD010


title: PIT_P1-PIT_P2-D9-AD004


title: PIT_P5-PIT_P6-A7-AD008


title: PIT_P7-PIT_P8-B4-AD010


title: PIT_P1-PIT_P2-D9-AD007


title: PIT_P1-PIT_P2-E2-AD012


title: PIT_P5-PIT_P6-B5-AD008


title: PIT_P1-PIT_P2-G6-AD012


title: PIT_P5-PIT_P6-F2-AD007


title: PIT_P5-PIT_P6-A11-AD002


title: PIT_P5-PIT_P6-F8-AD008


title: PIT_P3-PIT_P4-B9-AD004


title: PIT_P5-PIT_P6-E11-AD006


title: PIT_P1-PIT_P2-B1-AD012


title: PIT_P5-PIT_P6-D1-AD008


title: PIT_P5-PIT_P6-D10-AD002


title: PIT_P3-PIT_P4-A6-AD006


title: PIT_P3-PIT_P4-F9-AD002


title: PIT_P3-PIT_P4-C8-AD002


title: PIT_P5-PIT_P6-H6-AD001


title: PIT_P5-PIT_P6-C12-AD006


title: PIT_P1-PIT_P2-F3-AD006


title: PIT_P5-PIT_P6-G6-AD002


title: PIT_P7-PIT_P8-C10-AD007


title: PIT_P1-PIT_P2-G7-AD004


title: PIT_P7-PIT_P8-A3-AD006


title: PIT_P3-PIT_P4-G9-AD008


title: PIT_P7-PIT_P8-A8-AD012


title: PIT_P7-PIT_P8-C4-AD007


title: PIT_P5-PIT_P6-A5-AD010


title: PIT_P1-PIT_P2-B5-AD006


title: PIT_P3-PIT_P4-E9-AD002


title: PIT_P3-PIT_P4-E2-AD006


title: PIT_P1-PIT_P2-A9-AD004


title: PIT_P1-PIT_P2-G9-AD004


title: PIT_P7-PIT_P8-C1-AD007


title: PIT_P5-PIT_P6-H8-AD002


title: PIT_P5-PIT_P6-E11-AD012


title: PIT_P7-PIT_P8-D3-AD012


title: PIT_P5-PIT_P6-D9-AD008


title: PIT_P3-PIT_P4-F12-AD001


title: PIT_P1-PIT_P2-C12-AD010


title: PIT_P5-PIT_P6-A4-AD006


title: PIT_P7-PIT_P8-G5-AD004


title: PIT_P3-PIT_P4-G4-AD012


title: PIT_P5-PIT_P6-C9-AD001


title: PIT_P7-PIT_P8-B7-AD012


title: PIT_P3-PIT_P4-H11-AD004


title: PIT_P7-PIT_P8-A11-AD008


title: PIT_P1-PIT_P2-B2-AD008


title: PIT_P1-PIT_P2-B8-AD008


title: PIT_P5-PIT_P6-B4-AD007


title: PIT_P3-PIT_P4-F7-AD001


title: PIT_P3-PIT_P4-B8-AD007


title: PIT_P7-PIT_P8-H12-AD007


title: PIT_P3-PIT_P4-G8-AD001


title: PIT_P5-PIT_P6-F3-AD008


title: PIT_P5-PIT_P6-C2-AD012


title: PIT_P7-PIT_P8-B5-AD010


title: PIT_P3-PIT_P4-H1-AD007


title: PIT_P7-PIT_P8-G9-AD002


title: PIT_P1-PIT_P2-F8-AD002


title: PIT_P5-PIT_P6-F2-AD010


title: PIT_P3-PIT_P4-E3-AD001


title: PIT_P5-PIT_P6-B5-AD007


title: PIT_P1-PIT_P2-B9-AD006


title: PIT_P7-PIT_P8-F1-AD008


title: PIT_P7-PIT_P8-D3-AD002


title: PIT_P1-PIT_P2-D8-AD008


title: PIT_P7-PIT_P8-C2-AD008


title: PIT_P5-PIT_P6-B11-AD008


title: PIT_P1-PIT_P2-H8-AD010


title: PIT_P5-PIT_P6-G8-AD006


title: PIT_P3-PIT_P4-B11-AD008


title: PIT_P3-PIT_P4-H7-AD010


title: PIT_P1-PIT_P2-A4-AD010


title: PIT_P1-PIT_P2-B6-AD002


title: PIT_P3-PIT_P4-B1-AD006


title: PIT_P5-PIT_P6-H11-AD008


title: PIT_P7-PIT_P8-B4-AD002


title: PIT_P3-PIT_P4-G12-AD006


title: PIT_P1-PIT_P2-D4-AD001


title: PIT_P7-PIT_P8-B11-AD007


title: PIT_P3-PIT_P4-D4-AD010


title: PIT_P1-PIT_P2-B10-AD004


title: PIT_P7-PIT_P8-B3-AD012


title: PIT_P1-PIT_P2-C9-AD006


title: PIT_P7-PIT_P8-D7-AD008


title: PIT_P1-PIT_P2-H10-AD004


title: PIT_P3-PIT_P4-B4-AD012


title: PIT_P3-PIT_P4-H6-AD012


title: PIT_P3-PIT_P4-H9-AD010


title: PIT_P5-PIT_P6-B7-AD007


title: PIT_P3-PIT_P4-F7-AD002


title: PIT_P3-PIT_P4-E8-AD006


title: PIT_P7-PIT_P8-H12-AD010


title: PIT_P1-PIT_P2-G3-AD001


title: PIT_P5-PIT_P6-C6-AD010


title: PIT_P7-PIT_P8-G8-AD004


title: PIT_P1-PIT_P2-B12-AD007


title: PIT_P1-PIT_P2-A10-AD002


title: PIT_P3-PIT_P4-D10-AD012


title: PIT_P1-PIT_P2-F11-AD004


title: PIT_P1-PIT_P2-F8-AD001


title: PIT_P3-PIT_P4-G5-AD006


title: PIT_P1-PIT_P2-A2-AD012


title: PIT_P7-PIT_P8-H6-AD001


title: PIT_P3-PIT_P4-C4-AD012


title: PIT_P7-PIT_P8-D11-AD012


title: PIT_P7-PIT_P8-H4-AD006


title: PIT_P7-PIT_P8-C8-AD012


title: PIT_P7-PIT_P8-C2-AD007


title: PIT_P3-PIT_P4-H6-AD002


title: PIT_P1-PIT_P2-C1-AD006


title: PIT_P1-PIT_P2-C4-AD002


title: PIT_P7-PIT_P8-C7-AD012


title: PIT_P3-PIT_P4-D5-AD008


title: PIT_P5-PIT_P6-F12-AD002


title: PIT_P3-PIT_P4-F8-AD004


title: PIT_P5-PIT_P6-D9-AD004


title: PIT_P7-PIT_P8-H7-AD006


title: PIT_P1-PIT_P2-A10-AD008


title: PIT_P1-PIT_P2-E9-AD001


title: PIT_P5-PIT_P6-E7-AD012


title: PIT_P1-PIT_P2-D2-AD004


title: PIT_P3-PIT_P4-A5-AD012


title: PIT_P7-PIT_P8-G5-AD012


title: PIT_P7-PIT_P8-C3-AD007


title: PIT_P3-PIT_P4-E7-AD012


title: PIT_P5-PIT_P6-C3-AD012


title: PIT_P3-PIT_P4-G11-AD008


title: PIT_P1-PIT_P2-C2-AD008


title: PIT_P5-PIT_P6-B9-AD010


title: PIT_P7-PIT_P8-A8-AD001


title: PIT_P5-PIT_P6-C6-AD006


title: PIT_P1-PIT_P2-H7-AD001


title: PIT_P7-PIT_P8-G11-AD012


title: PIT_P7-PIT_P8-E11-AD010


title: PIT_P7-PIT_P8-D2-AD010


title: PIT_P1-PIT_P2-A3-AD008


title: PIT_P3-PIT_P4-H8-AD006


title: PIT_P7-PIT_P8-F1-AD004


title: PIT_P5-PIT_P6-A4-AD008


title: PIT_P1-PIT_P2-F4-AD007


title: PIT_P7-PIT_P8-G2-AD012


title: PIT_P3-PIT_P4-F4-AD008


title: PIT_P7-PIT_P8-H12-AD012


title: PIT_P7-PIT_P8-G9-AD010


title: PIT_P3-PIT_P4-C2-AD006


title: PIT_P7-PIT_P8-C8-AD006


title: PIT_P3-PIT_P4-A7-AD007


title: PIT_P3-PIT_P4-F7-AD007


title: PIT_P3-PIT_P4-E12-AD006


title: PIT_P7-PIT_P8-E9-AD001


title: PIT_P7-PIT_P8-D10-AD007


title: PIT_P1-PIT_P2-G2-AD006


title: PIT_P7-PIT_P8-D4-AD004


title: PIT_P7-PIT_P8-F1-AD007


title: PIT_P3-PIT_P4-G10-AD010


title: PIT_P7-PIT_P8-D9-AD012


title: PIT_P5-PIT_P6-F8-AD002


title: PIT_P7-PIT_P8-A1-AD012


title: PIT_P7-PIT_P8-H1-AD007


title: PIT_P7-PIT_P8-D1-AD004


title: PIT_P3-PIT_P4-H3-AD007


title: PIT_P3-PIT_P4-A6-AD001


title: PIT_P3-PIT_P4-C7-AD004


title: PIT_P3-PIT_P4-D8-AD007


title: PIT_P3-PIT_P4-C1-AD006


title: PIT_P7-PIT_P8-G4-AD007


title: PIT_P7-PIT_P8-F1-AD001


title: PIT_P7-PIT_P8-A7-AD007


title: PIT_P7-PIT_P8-A12-AD012


title: PIT_P7-PIT_P8-C7-AD007


title: PIT_P3-PIT_P4-E4-AD007


title: PIT_P3-PIT_P4-F2-AD004


title: PIT_P1-PIT_P2-E7-AD002


title: PIT_P1-PIT_P2-G5-AD006


title: PIT_P5-PIT_P6-F10-AD008


title: PIT_P7-PIT_P8-A3-AD010


title: PIT_P7-PIT_P8-G10-AD008


title: PIT_P3-PIT_P4-F9-AD012


title: PIT_P5-PIT_P6-B4-AD010


title: PIT_P3-PIT_P4-D3-AD012


title: PIT_P7-PIT_P8-C7-AD001


title: PIT_P1-PIT_P2-H10-AD001


title: PIT_P5-PIT_P6-C3-AD007


title: PIT_P7-PIT_P8-B11-AD004


title: PIT_P5-PIT_P6-E7-AD007


title: PIT_P3-PIT_P4-B4-AD004


title: PIT_P1-PIT_P2-B4-AD008


title: PIT_P3-PIT_P4-C12-AD010


title: PIT_P7-PIT_P8-B11-AD001


title: PIT_P3-PIT_P4-D10-AD007


title: PIT_P1-PIT_P2-D12-AD002


title: PIT_P1-PIT_P2-B5-AD002


title: PIT_P1-PIT_P2-D7-AD002


title: PIT_P3-PIT_P4-H1-AD002


title: PIT_P3-PIT_P4-G8-AD006


title: PIT_P1-PIT_P2-E10-AD001


title: PIT_P7-PIT_P8-C6-AD006


title: PIT_P3-PIT_P4-A7-AD004


title: PIT_P3-PIT_P4-E8-AD004


title: PIT_P7-PIT_P8-G5-AD001


title: PIT_P1-PIT_P2-B3-AD012


title: PIT_P5-PIT_P6-B11-AD006


title: PIT_P7-PIT_P8-D4-AD002


title: PIT_P3-PIT_P4-D9-AD010


title: PIT_P1-PIT_P2-F5-AD001


title: PIT_P7-PIT_P8-E2-AD006


title: PIT_P5-PIT_P6-A1-AD007


title: PIT_P3-PIT_P4-C11-AD010


title: PIT_P7-PIT_P8-C3-AD012


title: PIT_P1-PIT_P2-B3-AD001


title: PIT_P1-PIT_P2-A8-AD002


title: PIT_P1-PIT_P2-B3-AD010


title: PIT_P5-PIT_P6-F1-AD012


title: PIT_P1-PIT_P2-D7-AD012


title: PIT_P5-PIT_P6-C4-AD001


title: PIT_P5-PIT_P6-G7-AD007


title: PIT_P5-PIT_P6-F7-AD010


title: PIT_P1-PIT_P2-E5-AD010


title: PIT_P5-PIT_P6-C1-AD012


title: PIT_P1-PIT_P2-G12-AD001


title: PIT_P5-PIT_P6-C2-AD004


title: PIT_P5-PIT_P6-D1-AD010


title: PIT_P7-PIT_P8-H7-AD010


title: PIT_P1-PIT_P2-C2-AD007


title: PIT_P5-PIT_P6-C7-AD012


title: PIT_P1-PIT_P2-D12-AD012


title: PIT_P3-PIT_P4-E12-AD008


title: PIT_P5-PIT_P6-D7-AD012


title: PIT_P7-PIT_P8-C4-AD008


title: PIT_P7-PIT_P8-C4-AD012


title: PIT_P3-PIT_P4-H5-AD008


title: PIT_P7-PIT_P8-D8-AD001


title: PIT_P1-PIT_P2-B6-AD001


title: PIT_P5-PIT_P6-D7-AD008


title: PIT_P1-PIT_P2-E8-AD006


title: PIT_P5-PIT_P6-H9-AD006


title: PIT_P5-PIT_P6-C3-AD001


title: PIT_P5-PIT_P6-H7-AD002


title: PIT_P1-PIT_P2-G7-AD008


title: PIT_P3-PIT_P4-F10-AD010


title: PIT_P3-PIT_P4-A9-AD002


title: PIT_P3-PIT_P4-E11-AD007


title: PIT_P1-PIT_P2-H10-AD010


title: PIT_P1-PIT_P2-F2-AD002


title: PIT_P3-PIT_P4-C3-AD010


title: PIT_P1-PIT_P2-H4-AD006


title: PIT_P7-PIT_P8-H1-AD001


title: PIT_P7-PIT_P8-G10-AD007


title: PIT_P5-PIT_P6-B2-AD002


title: PIT_P3-PIT_P4-C7-AD006


title: PIT_P1-PIT_P2-G12-AD008


title: PIT_P7-PIT_P8-D6-AD012


title: PIT_P1-PIT_P2-B7-AD001


title: PIT_P1-PIT_P2-E12-AD010


title: PIT_P1-PIT_P2-F10-AD010


title: PIT_P3-PIT_P4-F10-AD008


title: PIT_P3-PIT_P4-A7-AD006


title: PIT_P5-PIT_P6-D9-AD006


title: PIT_P5-PIT_P6-C7-AD010


title: PIT_P5-PIT_P6-F6-AD001


title: PIT_P1-PIT_P2-E3-AD002


title: PIT_P7-PIT_P8-E10-AD004


title: PIT_P1-PIT_P2-D12-AD001


title: PIT_P5-PIT_P6-D3-AD008


title: PIT_P7-PIT_P8-D7-AD007


title: PIT_P3-PIT_P4-E6-AD007


title: PIT_P3-PIT_P4-E9-AD006


title: PIT_P1-PIT_P2-B7-AD010


title: PIT_P3-PIT_P4-A2-AD010


title: PIT_P5-PIT_P6-G9-AD002


title: PIT_P7-PIT_P8-C6-AD007


title: PIT_P7-PIT_P8-C12-AD006


title: PIT_P5-PIT_P6-B7-AD008


title: PIT_P7-PIT_P8-A6-AD007


title: PIT_P1-PIT_P2-D12-AD004


title: PIT_P5-PIT_P6-B5-AD012


title: PIT_P1-PIT_P2-B2-AD002


title: PIT_P5-PIT_P6-B7-AD006


title: PIT_P3-PIT_P4-D4-AD012


title: PIT_P7-PIT_P8-E1-AD001


title: PIT_P1-PIT_P2-C5-AD007


title: PIT_P7-PIT_P8-F9-AD001


title: PIT_P5-PIT_P6-H8-AD006


title: PIT_P3-PIT_P4-A5-AD008


title: PIT_P5-PIT_P6-A11-AD010


title: PIT_P1-PIT_P2-H8-AD006


title: PIT_P5-PIT_P6-H4-AD008


title: PIT_P5-PIT_P6-E3-AD010


title: PIT_P1-PIT_P2-A11-AD010


title: PIT_P7-PIT_P8-H11-AD008


title: PIT_P1-PIT_P2-C2-AD012


title: PIT_P7-PIT_P8-E5-AD008


title: PIT_P7-PIT_P8-H5-AD007


title: PIT_P1-PIT_P2-C4-AD006


title: PIT_P1-PIT_P2-H9-AD012


title: PIT_P5-PIT_P6-G8-AD001


title: PIT_P3-PIT_P4-F7-AD006


title: PIT_P1-PIT_P2-F2-AD006


title: PIT_P3-PIT_P4-F3-AD004


title: PIT_P3-PIT_P4-G5-AD002


title: PIT_P7-PIT_P8-D12-AD006


title: PIT_P1-PIT_P2-H7-AD004


title: PIT_P5-PIT_P6-A12-AD001


title: PIT_P1-PIT_P2-A4-AD012


title: PIT_P3-PIT_P4-C10-AD006


title: PIT_P3-PIT_P4-E5-AD012


title: PIT_P7-PIT_P8-H2-AD007


title: PIT_P5-PIT_P6-F11-AD001


title: PIT_P3-PIT_P4-D5-AD001


title: PIT_P1-PIT_P2-E8-AD008


title: PIT_P3-PIT_P4-H10-AD010


title: PIT_P5-PIT_P6-B4-AD012


title: PIT_P7-PIT_P8-D12-AD002


title: PIT_P1-PIT_P2-H7-AD007


title: PIT_P1-PIT_P2-H1-AD004


title: PIT_P1-PIT_P2-G11-AD001


title: PIT_P7-PIT_P8-A11-AD007


title: PIT_P3-PIT_P4-G1-AD002


title: PIT_P5-PIT_P6-B12-AD002


title: PIT_P3-PIT_P4-D2-AD004


title: PIT_P3-PIT_P4-H12-AD008


title: PIT_P7-PIT_P8-E7-AD001


title: PIT_P5-PIT_P6-E4-AD006


title: PIT_P5-PIT_P6-C6-AD004


title: PIT_P1-PIT_P2-H9-AD007


title: PIT_P5-PIT_P6-H1-AD002


title: PIT_P5-PIT_P6-A3-AD006


title: PIT_P3-PIT_P4-B12-AD008


title: PIT_P7-PIT_P8-C6-AD001


title: PIT_P3-PIT_P4-G1-AD004


title: PIT_P5-PIT_P6-F7-AD008


title: PIT_P7-PIT_P8-F10-AD001


title: PIT_P5-PIT_P6-F9-AD012


title: PIT_P5-PIT_P6-G5-AD008


title: PIT_P3-PIT_P4-B2-AD012


title: PIT_P3-PIT_P4-B7-AD010


title: PIT_P7-PIT_P8-F12-AD004


title: PIT_P3-PIT_P4-D12-AD002


title: PIT_P3-PIT_P4-G9-AD002


title: PIT_P5-PIT_P6-A6-AD001


title: PIT_P5-PIT_P6-A11-AD008


title: PIT_P3-PIT_P4-C7-AD008


title: PIT_P3-PIT_P4-A10-AD012


title: PIT_P3-PIT_P4-C9-AD007


title: PIT_P1-PIT_P2-G7-AD007


title: PIT_P3-PIT_P4-F10-AD001


title: PIT_P1-PIT_P2-A3-AD004


title: PIT_P7-PIT_P8-F11-AD006


title: PIT_P5-PIT_P6-D12-AD002


title: PIT_P3-PIT_P4-G12-AD004


title: PIT_P1-PIT_P2-C10-AD012


title: PIT_P7-PIT_P8-E1-AD010


title: PIT_P1-PIT_P2-G6-AD008


title: PIT_P7-PIT_P8-B12-AD006


title: PIT_P3-PIT_P4-E2-AD002


title: PIT_P7-PIT_P8-D12-AD007


title: PIT_P3-PIT_P4-E10-AD001


title: PIT_P3-PIT_P4-C11-AD004


title: PIT_P5-PIT_P6-H10-AD002


title: PIT_P3-PIT_P4-H10-AD008


title: PIT_P3-PIT_P4-B11-AD002


title: PIT_P5-PIT_P6-A12-AD002


title: PIT_P1-PIT_P2-H5-AD010


title: PIT_P5-PIT_P6-F10-AD006


title: PIT_P3-PIT_P4-D8-AD008


title: PIT_P5-PIT_P6-G7-AD002


title: PIT_P5-PIT_P6-E9-AD012


title: PIT_P1-PIT_P2-D9-AD006


title: PIT_P1-PIT_P2-F6-AD012


title: PIT_P3-PIT_P4-F2-AD012


title: PIT_P7-PIT_P8-A7-AD012


title: PIT_P3-PIT_P4-C6-AD007


title: PIT_P1-PIT_P2-F5-AD002


title: PIT_P7-PIT_P8-F12-AD007


title: PIT_P3-PIT_P4-E9-AD010


title: PIT_P5-PIT_P6-C11-AD010


title: PIT_P5-PIT_P6-H12-AD001


title: PIT_P5-PIT_P6-B8-AD007


title: PIT_P5-PIT_P6-C8-AD008


title: PIT_P1-PIT_P2-G2-AD004


title: PIT_P1-PIT_P2-A10-AD001


title: PIT_P5-PIT_P6-A3-AD008


title: PIT_P3-PIT_P4-D9-AD012


title: PIT_P3-PIT_P4-B7-AD002


title: PIT_P7-PIT_P8-F3-AD010


title: PIT_P5-PIT_P6-H9-AD008


title: PIT_P7-PIT_P8-C1-AD008


title: PIT_P3-PIT_P4-F5-AD004


title: PIT_P1-PIT_P2-E9-AD012


title: PIT_P3-PIT_P4-E8-AD008


title: PIT_P5-PIT_P6-G6-AD007


title: PIT_P3-PIT_P4-A8-AD010


title: PIT_P1-PIT_P2-G10-AD001


title: PIT_P5-PIT_P6-G8-AD008


title: PIT_P1-PIT_P2-B12-AD010


title: PIT_P5-PIT_P6-H7-AD007


title: PIT_P5-PIT_P6-H11-AD002


title: PIT_P1-PIT_P2-D4-AD006


title: PIT_P3-PIT_P4-F7-AD010


title: PIT_P7-PIT_P8-D8-AD007


title: PIT_P3-PIT_P4-A9-AD008


title: PIT_P5-PIT_P6-G9-AD007


title: PIT_P3-PIT_P4-B8-AD004


title: PIT_P7-PIT_P8-B11-AD012


title: PIT_P3-PIT_P4-H5-AD006


title: PIT_P5-PIT_P6-D5-AD007


title: PIT_P7-PIT_P8-F4-AD006


title: PIT_P5-PIT_P6-G3-AD008


title: PIT_P3-PIT_P4-F9-AD004


title: PIT_P3-PIT_P4-D1-AD004


title: PIT_P3-PIT_P4-C4-AD010


title: PIT_P3-PIT_P4-G8-AD004


title: PIT_P7-PIT_P8-F9-AD004


title: PIT_P3-PIT_P4-E10-AD008


title: PIT_P1-PIT_P2-C5-AD004


title: PIT_P1-PIT_P2-G9-AD010


title: PIT_P3-PIT_P4-D5-AD010


title: PIT_P3-PIT_P4-E11-AD004


title: PIT_P5-PIT_P6-E4-AD001


title: PIT_P1-PIT_P2-A3-AD001


title: PIT_P7-PIT_P8-A8-AD010


title: PIT_P1-PIT_P2-B1-AD008


title: PIT_P1-PIT_P2-G4-AD006


title: PIT_P7-PIT_P8-G1-AD002


title: PIT_P7-PIT_P8-G1-AD008


title: PIT_P3-PIT_P4-C10-AD012


title: PIT_P5-PIT_P6-E7-AD008


title: PIT_P5-PIT_P6-C10-AD001


title: PIT_P3-PIT_P4-F8-AD007


title: PIT_P1-PIT_P2-F9-AD002


title: PIT_P5-PIT_P6-A10-AD008


title: PIT_P1-PIT_P2-F7-AD002


title: PIT_P3-PIT_P4-G9-AD012


title: PIT_P3-PIT_P4-H3-AD008


title: PIT_P5-PIT_P6-G2-AD010


title: PIT_P7-PIT_P8-E7-AD002


title: PIT_P1-PIT_P2-A12-AD006


title: PIT_P1-PIT_P2-E4-AD010


title: PIT_P3-PIT_P4-E12-AD010


title: PIT_P3-PIT_P4-F11-AD008


title: PIT_P3-PIT_P4-E7-AD008


title: PIT_P1-PIT_P2-H3-AD012


title: PIT_P3-PIT_P4-H9-AD002


title: PIT_P5-PIT_P6-F11-AD012


title: PIT_P5-PIT_P6-A10-AD004


title: PIT_P7-PIT_P8-C5-AD008


title: PIT_P1-PIT_P2-A8-AD010


title: PIT_P1-PIT_P2-G3-AD010


title: PIT_P1-PIT_P2-B9-AD004


title: PIT_P3-PIT_P4-H7-AD001


title: PIT_P1-PIT_P2-C8-AD004


title: PIT_P5-PIT_P6-G6-AD001


title: PIT_P3-PIT_P4-H7-AD004


title: PIT_P3-PIT_P4-F4-AD012


title: PIT_P1-PIT_P2-G8-AD006


title: PIT_P5-PIT_P6-G7-AD008


title: PIT_P3-PIT_P4-B11-AD001


title: PIT_P1-PIT_P2-G5-AD004


title: PIT_P1-PIT_P2-A7-AD001


title: PIT_P1-PIT_P2-G7-AD012


title: PIT_P7-PIT_P8-A4-AD010


title: PIT_P7-PIT_P8-E1-AD002


title: PIT_P1-PIT_P2-A10-AD004


title: PIT_P7-PIT_P8-G9-AD008


title: PIT_P1-PIT_P2-A10-AD012


title: PIT_P1-PIT_P2-B6-AD012


title: PIT_P7-PIT_P8-D10-AD002


title: PIT_P7-PIT_P8-C2-AD006


title: PIT_P3-PIT_P4-G7-AD012


title: PIT_P7-PIT_P8-D9-AD007


title: PIT_P7-PIT_P8-B12-AD002


title: PIT_P1-PIT_P2-H3-AD002


title: PIT_P1-PIT_P2-E4-AD008


title: PIT_P7-PIT_P8-E9-AD012


title: PIT_P1-PIT_P2-B2-AD012


title: PIT_P3-PIT_P4-B1-AD008


title: PIT_P3-PIT_P4-H7-AD012


title: PIT_P3-PIT_P4-B9-AD010


title: PIT_P5-PIT_P6-D5-AD008


title: PIT_P3-PIT_P4-A2-AD012


title: PIT_P3-PIT_P4-G12-AD007


title: PIT_P5-PIT_P6-H2-AD004


title: PIT_P3-PIT_P4-C12-AD007


title: PIT_P5-PIT_P6-H2-AD008


title: PIT_P5-PIT_P6-B2-AD008


title: PIT_P7-PIT_P8-C10-AD006


title: PIT_P1-PIT_P2-H10-AD006


title: PIT_P1-PIT_P2-D2-AD012


title: PIT_P5-PIT_P6-E6-AD002


title: PIT_P1-PIT_P2-H6-AD007


title: PIT_P7-PIT_P8-A7-AD002


title: PIT_P7-PIT_P8-F9-AD010


title: PIT_P3-PIT_P4-E6-AD008


title: PIT_P3-PIT_P4-B10-AD007


title: PIT_P3-PIT_P4-F1-AD007


title: PIT_P5-PIT_P6-B11-AD002


title: PIT_P5-PIT_P6-B2-AD004


title: PIT_P5-PIT_P6-E8-AD006


title: PIT_P5-PIT_P6-F2-AD006


title: PIT_P7-PIT_P8-E4-AD012


title: PIT_P7-PIT_P8-G12-AD008


title: PIT_P1-PIT_P2-H8-AD002


title: PIT_P1-PIT_P2-D2-AD007


title: PIT_P1-PIT_P2-G4-AD007


title: PIT_P3-PIT_P4-B7-AD004


title: PIT_P5-PIT_P6-H11-AD001


title: PIT_P3-PIT_P4-E10-AD010


title: PIT_P1-PIT_P2-F10-AD004


title: PIT_P5-PIT_P6-F8-AD001


title: PIT_P5-PIT_P6-C2-AD010


title: PIT_P7-PIT_P8-H6-AD008


title: PIT_P5-PIT_P6-F9-AD002


title: PIT_P7-PIT_P8-C3-AD004


title: PIT_P1-PIT_P2-D5-AD001


title: PIT_P3-PIT_P4-D5-AD007


title: PIT_P7-PIT_P8-A1-AD004


title: PIT_P1-PIT_P2-C10-AD008


title: PIT_P3-PIT_P4-B7-AD012


title: PIT_P5-PIT_P6-D3-AD002


title: PIT_P1-PIT_P2-G6-AD007


title: PIT_P3-PIT_P4-A8-AD012


title: PIT_P7-PIT_P8-H9-AD008


title: PIT_P1-PIT_P2-B12-AD002


title: PIT_P7-PIT_P8-G11-AD007


title: PIT_P7-PIT_P8-G12-AD010


title: PIT_P3-PIT_P4-E4-AD001


title: PIT_P7-PIT_P8-F8-AD012


title: PIT_P7-PIT_P8-E4-AD004


title: PIT_P1-PIT_P2-E9-AD010


title: PIT_P7-PIT_P8-H12-AD008


title: PIT_P1-PIT_P2-G6-AD010


title: PIT_P7-PIT_P8-H11-AD001


title: PIT_P7-PIT_P8-F3-AD002


title: PIT_P3-PIT_P4-B5-AD001


title: PIT_P7-PIT_P8-C12-AD007


title: PIT_P5-PIT_P6-F2-AD002


title: PIT_P5-PIT_P6-E4-AD012


title: PIT_P1-PIT_P2-G3-AD002


title: PIT_P1-PIT_P2-D7-AD010


title: PIT_P3-PIT_P4-G2-AD002


title: PIT_P7-PIT_P8-D1-AD002


title: PIT_P3-PIT_P4-D6-AD012


title: PIT_P3-PIT_P4-F5-AD006


title: PIT_P5-PIT_P6-E6-AD004


title: PIT_P3-PIT_P4-E3-AD004


title: PIT_P5-PIT_P6-G9-AD012


title: PIT_P1-PIT_P2-A5-AD004


title: PIT_P7-PIT_P8-B11-AD006


title: PIT_P7-PIT_P8-A5-AD002


title: PIT_P1-PIT_P2-C6-AD001


title: PIT_P7-PIT_P8-F1-AD002


title: PIT_P1-PIT_P2-B3-AD002


title: PIT_P5-PIT_P6-H12-AD002


title: PIT_P7-PIT_P8-C2-AD004


title: PIT_P7-PIT_P8-D9-AD002


title: PIT_P3-PIT_P4-F9-AD007


title: PIT_P3-PIT_P4-E9-AD008


title: PIT_P1-PIT_P2-F12-AD006


title: PIT_P1-PIT_P2-C6-AD012


title: PIT_P1-PIT_P2-C12-AD001


title: PIT_P5-PIT_P6-G10-AD004


title: PIT_P1-PIT_P2-C6-AD008


title: PIT_P1-PIT_P2-C5-AD006


title: PIT_P7-PIT_P8-H6-AD007


title: PIT_P1-PIT_P2-F11-AD001


title: PIT_P5-PIT_P6-A11-AD001


title: PIT_P7-PIT_P8-F6-AD008


title: PIT_P5-PIT_P6-B7-AD001


title: PIT_P3-PIT_P4-F2-AD002


title: PIT_P1-PIT_P2-H10-AD008


title: PIT_P7-PIT_P8-B8-AD012


title: PIT_P5-PIT_P6-F4-AD001


title: PIT_P3-PIT_P4-D2-AD008


title: PIT_P5-PIT_P6-H6-AD004


title: PIT_P1-PIT_P2-H7-AD008


title: PIT_P7-PIT_P8-A4-AD002


title: PIT_P7-PIT_P8-G1-AD007


title: PIT_P3-PIT_P4-G7-AD006


title: PIT_P5-PIT_P6-G2-AD006


title: PIT_P5-PIT_P6-H7-AD012


title: PIT_P1-PIT_P2-F9-AD012


title: PIT_P1-PIT_P2-B1-AD007


title: PIT_P5-PIT_P6-H5-AD007


title: PIT_P3-PIT_P4-A9-AD007


title: PIT_P1-PIT_P2-E4-AD012


title: PIT_P5-PIT_P6-G6-AD004


title: PIT_P7-PIT_P8-C7-AD006


title: PIT_P7-PIT_P8-B1-AD004


title: PIT_P7-PIT_P8-B5-AD004


title: PIT_P7-PIT_P8-G8-AD007


title: PIT_P1-PIT_P2-E12-AD002


title: PIT_P5-PIT_P6-G2-AD012


title: PIT_P3-PIT_P4-C4-AD008


title: PIT_P1-PIT_P2-H5-AD001


title: PIT_P3-PIT_P4-C4-AD007


title: PIT_P5-PIT_P6-E2-AD012


title: PIT_P7-PIT_P8-H5-AD002


title: PIT_P3-PIT_P4-B5-AD007


title: PIT_P7-PIT_P8-C12-AD002


title: PIT_P1-PIT_P2-F10-AD012


title: PIT_P7-PIT_P8-D2-AD006


title: PIT_P5-PIT_P6-A8-AD008


title: PIT_P3-PIT_P4-F5-AD001


title: PIT_P5-PIT_P6-C1-AD008


title: PIT_P1-PIT_P2-C11-AD002


title: PIT_P3-PIT_P4-D5-AD002


title: PIT_P1-PIT_P2-H2-AD004


title: PIT_P5-PIT_P6-F11-AD007


title: PIT_P1-PIT_P2-G3-AD006


title: PIT_P5-PIT_P6-E10-AD008


title: PIT_P5-PIT_P6-E12-AD006


title: PIT_P7-PIT_P8-G2-AD002


title: PIT_P5-PIT_P6-E5-AD008


title: PIT_P5-PIT_P6-E9-AD001


title: PIT_P1-PIT_P2-G10-AD002


title: PIT_P1-PIT_P2-C3-AD006


title: PIT_P5-PIT_P6-D11-AD012


title: PIT_P5-PIT_P6-E4-AD008


title: PIT_P3-PIT_P4-A3-AD006


title: PIT_P1-PIT_P2-D1-AD004


title: PIT_P7-PIT_P8-B9-AD008


title: PIT_P7-PIT_P8-D4-AD012


title: PIT_P3-PIT_P4-F2-AD007


title: PIT_P1-PIT_P2-D1-AD002


title: PIT_P5-PIT_P6-C5-AD001


title: PIT_P1-PIT_P2-H6-AD012


title: PIT_P1-PIT_P2-C12-AD002


title: PIT_P3-PIT_P4-H4-AD001


title: PIT_P3-PIT_P4-G4-AD010


title: PIT_P5-PIT_P6-C10-AD004


title: PIT_P3-PIT_P4-A4-AD006


title: PIT_P7-PIT_P8-H10-AD001


title: PIT_P7-PIT_P8-E12-AD001


title: PIT_P7-PIT_P8-H1-AD002


title: PIT_P7-PIT_P8-E11-AD001


title: PIT_P5-PIT_P6-H10-AD012


title: PIT_P5-PIT_P6-G7-AD006


title: PIT_P1-PIT_P2-F7-AD010


title: PIT_P1-PIT_P2-F10-AD007


title: PIT_P7-PIT_P8-C9-AD002


title: PIT_P1-PIT_P2-E1-AD012


title: PIT_P3-PIT_P4-A1-AD006


title: PIT_P7-PIT_P8-A8-AD007


title: PIT_P5-PIT_P6-E12-AD004


title: PIT_P7-PIT_P8-A12-AD007


title: PIT_P3-PIT_P4-G1-AD007


title: PIT_P1-PIT_P2-F11-AD010


title: PIT_P3-PIT_P4-A11-AD002


title: PIT_P3-PIT_P4-A2-AD004


title: PIT_P3-PIT_P4-E4-AD002


title: PIT_P5-PIT_P6-C5-AD008


title: PIT_P7-PIT_P8-E10-AD012


title: PIT_P3-PIT_P4-C11-AD002


title: PIT_P1-PIT_P2-E4-AD002


title: PIT_P5-PIT_P6-C2-AD001


title: PIT_P1-PIT_P2-F9-AD008


title: PIT_P1-PIT_P2-G5-AD008


title: PIT_P3-PIT_P4-F12-AD006


title: PIT_P3-PIT_P4-F11-AD006


title: PIT_P5-PIT_P6-D2-AD002


title: PIT_P3-PIT_P4-C7-AD001


title: PIT_P5-PIT_P6-D11-AD010


title: PIT_P1-PIT_P2-E7-AD012


title: PIT_P1-PIT_P2-C12-AD012


title: PIT_P7-PIT_P8-G3-AD008


title: PIT_P5-PIT_P6-D6-AD008


title: PIT_P3-PIT_P4-D11-AD006


title: PIT_P3-PIT_P4-B11-AD006


title: PIT_P5-PIT_P6-F11-AD010


title: PIT_P7-PIT_P8-D11-AD006


title: PIT_P7-PIT_P8-D7-AD001


title: PIT_P5-PIT_P6-F3-AD012


title: PIT_P7-PIT_P8-B6-AD008


title: PIT_P7-PIT_P8-H8-AD004


title: PIT_P3-PIT_P4-A3-AD004


title: PIT_P7-PIT_P8-C9-AD012


title: PIT_P7-PIT_P8-E9-AD008


title: PIT_P5-PIT_P6-D6-AD006


title: PIT_P1-PIT_P2-F3-AD010


title: PIT_P5-PIT_P6-H4-AD010


title: PIT_P3-PIT_P4-C7-AD002


title: PIT_P3-PIT_P4-C7-AD012


title: PIT_P3-PIT_P4-G7-AD004


title: PIT_P7-PIT_P8-F10-AD010


title: PIT_P3-PIT_P4-A4-AD007


title: PIT_P3-PIT_P4-A5-AD004


title: PIT_P5-PIT_P6-G3-AD002


title: PIT_P1-PIT_P2-G1-AD012


title: PIT_P7-PIT_P8-D5-AD006


title: PIT_P1-PIT_P2-E2-AD010


title: PIT_P7-PIT_P8-B7-AD001


title: PIT_P3-PIT_P4-F4-AD007


title: PIT_P5-PIT_P6-C7-AD002


title: PIT_P7-PIT_P8-B5-AD007


title: PIT_P7-PIT_P8-A11-AD002


title: PIT_P1-PIT_P2-A11-AD004


title: PIT_P5-PIT_P6-A3-AD002


title: PIT_P7-PIT_P8-G6-AD008


title: PIT_P5-PIT_P6-A10-AD012


title: PIT_P5-PIT_P6-B10-AD010


title: PIT_P1-PIT_P2-A1-AD008


title: PIT_P5-PIT_P6-G2-AD004


title: PIT_P1-PIT_P2-A1-AD007


title: PIT_P5-PIT_P6-G10-AD012


title: PIT_P3-PIT_P4-C12-AD002


title: PIT_P5-PIT_P6-F6-AD012


title: PIT_P1-PIT_P2-D6-AD010


title: PIT_P3-PIT_P4-E11-AD012


title: PIT_P5-PIT_P6-E9-AD002


title: PIT_P3-PIT_P4-A6-AD008


title: PIT_P3-PIT_P4-B9-AD007


title: PIT_P3-PIT_P4-G1-AD006


title: PIT_P5-PIT_P6-A1-AD006


title: PIT_P5-PIT_P6-E12-AD008


title: PIT_P7-PIT_P8-A5-AD004


title: PIT_P3-PIT_P4-G11-AD004


title: PIT_P1-PIT_P2-C8-AD006


title: PIT_P5-PIT_P6-H10-AD010


title: PIT_P7-PIT_P8-B6-AD006


title: PIT_P7-PIT_P8-D8-AD008


title: PIT_P3-PIT_P4-A10-AD010


title: PIT_P1-PIT_P2-F9-AD007


title: PIT_P5-PIT_P6-E2-AD006


title: PIT_P7-PIT_P8-G12-AD001


title: PIT_P7-PIT_P8-H7-AD004


title: PIT_P1-PIT_P2-F7-AD007


title: PIT_P7-PIT_P8-A3-AD007


title: PIT_P7-PIT_P8-E7-AD010


title: PIT_P1-PIT_P2-D6-AD006


title: PIT_P3-PIT_P4-H8-AD004


title: PIT_P5-PIT_P6-G5-AD006


title: PIT_P7-PIT_P8-H10-AD002


title: PIT_P5-PIT_P6-E5-AD004


title: PIT_P5-PIT_P6-G9-AD004


title: PIT_P5-PIT_P6-F3-AD006


title: PIT_P7-PIT_P8-F9-AD002


title: PIT_P7-PIT_P8-C3-AD008


title: PIT_P1-PIT_P2-F8-AD008


title: PIT_P7-PIT_P8-D12-AD008


title: PIT_P3-PIT_P4-A4-AD004


title: PIT_P7-PIT_P8-G12-AD012


title: PIT_P1-PIT_P2-A9-AD001


title: PIT_P3-PIT_P4-B3-AD012


title: PIT_P7-PIT_P8-H2-AD008


title: PIT_P7-PIT_P8-C11-AD001


title: PIT_P1-PIT_P2-D6-AD012


title: PIT_P1-PIT_P2-D9-AD001


title: PIT_P1-PIT_P2-C4-AD007


title: PIT_P7-PIT_P8-A7-AD006


title: PIT_P7-PIT_P8-C8-AD008


title: PIT_P1-PIT_P2-C1-AD008


title: PIT_P7-PIT_P8-C2-AD001


title: PIT_P3-PIT_P4-G10-AD008


title: PIT_P3-PIT_P4-G2-AD001


title: PIT_P5-PIT_P6-H10-AD004


title: PIT_P3-PIT_P4-B4-AD010


title: PIT_P7-PIT_P8-H1-AD008


title: PIT_P3-PIT_P4-C6-AD010


title: PIT_P7-PIT_P8-F8-AD008


title: PIT_P1-PIT_P2-C5-AD012


title: PIT_P1-PIT_P2-B11-AD008


title: PIT_P3-PIT_P4-B12-AD001


title: PIT_P3-PIT_P4-C2-AD007


title: PIT_P1-PIT_P2-D9-AD012


title: PIT_P7-PIT_P8-G2-AD004


title: PIT_P7-PIT_P8-A5-AD012


title: PIT_P5-PIT_P6-E6-AD001


title: PIT_P3-PIT_P4-D4-AD007


title: PIT_P5-PIT_P6-B11-AD012


title: PIT_P7-PIT_P8-A1-AD007


title: PIT_P3-PIT_P4-D2-AD010


title: PIT_P7-PIT_P8-A4-AD007


title: PIT_P1-PIT_P2-B12-AD001


title: PIT_P3-PIT_P4-H1-AD001


title: PIT_P7-PIT_P8-E5-AD012


title: PIT_P5-PIT_P6-F5-AD002


title: PIT_P3-PIT_P4-F11-AD012


title: PIT_P3-PIT_P4-H10-AD006


title: PIT_P5-PIT_P6-A2-AD001


title: PIT_P7-PIT_P8-G7-AD004


title: PIT_P3-PIT_P4-D12-AD006


title: PIT_P5-PIT_P6-H11-AD007


title: PIT_P3-PIT_P4-G9-AD007


title: PIT_P1-PIT_P2-C5-AD001


title: PIT_P3-PIT_P4-G2-AD007


title: PIT_P3-PIT_P4-A12-AD010


title: PIT_P3-PIT_P4-E11-AD002


title: PIT_P7-PIT_P8-G3-AD004


title: PIT_P7-PIT_P8-D3-AD001


title: PIT_P5-PIT_P6-C3-AD002


title: PIT_P3-PIT_P4-B11-AD012


title: PIT_P5-PIT_P6-F10-AD007


title: PIT_P3-PIT_P4-F9-AD006


title: PIT_P3-PIT_P4-H1-AD008


title: PIT_P1-PIT_P2-F7-AD004


title: PIT_P3-PIT_P4-C3-AD001


title: PIT_P1-PIT_P2-E2-AD008


title: PIT_P3-PIT_P4-D3-AD001


title: PIT_P3-PIT_P4-A1-AD010


title: PIT_P3-PIT_P4-C10-AD010


title: PIT_P7-PIT_P8-E7-AD004


title: PIT_P3-PIT_P4-E2-AD008


title: PIT_P1-PIT_P2-C11-AD007


title: PIT_P3-PIT_P4-F2-AD010


title: PIT_P3-PIT_P4-H12-AD001


title: PIT_P7-PIT_P8-C10-AD008


title: PIT_P7-PIT_P8-E8-AD002


title: PIT_P3-PIT_P4-D11-AD002


title: PIT_P5-PIT_P6-A1-AD004


title: PIT_P5-PIT_P6-F5-AD001


title: PIT_P7-PIT_P8-H5-AD010


title: PIT_P1-PIT_P2-H5-AD008


title: PIT_P7-PIT_P8-H2-AD001


title: PIT_P3-PIT_P4-G2-AD008


title: PIT_P1-PIT_P2-D10-AD010


title: PIT_P1-PIT_P2-G2-AD008


title: PIT_P3-PIT_P4-D2-AD012


title: PIT_P3-PIT_P4-H10-AD012


title: PIT_P5-PIT_P6-B4-AD002


title: PIT_P1-PIT_P2-E5-AD006


title: PIT_P5-PIT_P6-D3-AD004


title: PIT_P1-PIT_P2-E7-AD010


title: PIT_P7-PIT_P8-F4-AD010


title: PIT_P3-PIT_P4-E1-AD002


title: PIT_P7-PIT_P8-E1-AD004


title: PIT_P7-PIT_P8-H4-AD004


title: PIT_P5-PIT_P6-H1-AD010


title: PIT_P7-PIT_P8-F3-AD008


title: PIT_P7-PIT_P8-D7-AD012


title: PIT_P7-PIT_P8-H4-AD007


title: PIT_P1-PIT_P2-F11-AD002


title: PIT_P3-PIT_P4-A10-AD001


title: PIT_P7-PIT_P8-G3-AD010


title: PIT_P7-PIT_P8-A4-AD001


title: PIT_P5-PIT_P6-G10-AD001


title: PIT_P1-PIT_P2-C9-AD002


title: PIT_P7-PIT_P8-A2-AD007


title: PIT_P5-PIT_P6-D5-AD002


title: PIT_P7-PIT_P8-B7-AD010


title: PIT_P1-PIT_P2-E11-AD010


title: PIT_P3-PIT_P4-E6-AD012


title: PIT_P1-PIT_P2-F5-AD010


title: PIT_P7-PIT_P8-B8-AD002


title: PIT_P3-PIT_P4-F10-AD012


title: PIT_P5-PIT_P6-F7-AD012


title: PIT_P1-PIT_P2-C10-AD006


title: PIT_P3-PIT_P4-D9-AD002


title: PIT_P7-PIT_P8-A10-AD008


title: PIT_P7-PIT_P8-A6-AD010


title: PIT_P3-PIT_P4-E6-AD001


title: PIT_P5-PIT_P6-B12-AD007


title: PIT_P3-PIT_P4-G4-AD002


title: PIT_P7-PIT_P8-F12-AD002


title: PIT_P3-PIT_P4-C9-AD008


title: PIT_P5-PIT_P6-G1-AD004


title: PIT_P7-PIT_P8-A1-AD002


title: PIT_P3-PIT_P4-A3-AD001


title: PIT_P3-PIT_P4-A9-AD004


title: PIT_P7-PIT_P8-A9-AD007


title: PIT_P1-PIT_P2-C7-AD010


title: PIT_P7-PIT_P8-B5-AD002


title: PIT_P7-PIT_P8-G4-AD008


title: PIT_P3-PIT_P4-G9-AD004


title: PIT_P1-PIT_P2-D10-AD008


title: PIT_P7-PIT_P8-H5-AD004


title: PIT_P3-PIT_P4-G5-AD008


title: PIT_P1-PIT_P2-A6-AD012


title: PIT_P1-PIT_P2-G5-AD001


title: PIT_P3-PIT_P4-B4-AD007


title: PIT_P7-PIT_P8-C9-AD007


title: PIT_P3-PIT_P4-E8-AD002


title: PIT_P7-PIT_P8-H1-AD010


title: PIT_P5-PIT_P6-D10-AD006


title: PIT_P5-PIT_P6-G6-AD006


title: PIT_P5-PIT_P6-F10-AD012


title: PIT_P1-PIT_P2-H12-AD012


title: PIT_P7-PIT_P8-C5-AD001


title: PIT_P3-PIT_P4-A3-AD008


title: PIT_P7-PIT_P8-B2-AD006


title: PIT_P3-PIT_P4-E4-AD012


title: PIT_P7-PIT_P8-H5-AD001


title: PIT_P5-PIT_P6-D8-AD002


title: PIT_P5-PIT_P6-A12-AD010


title: PIT_P5-PIT_P6-F11-AD006


title: PIT_P5-PIT_P6-B1-AD001


title: PIT_P1-PIT_P2-F2-AD007


title: PIT_P7-PIT_P8-B3-AD004


title: PIT_P3-PIT_P4-C8-AD001


title: PIT_P1-PIT_P2-H5-AD002


title: PIT_P7-PIT_P8-G10-AD001


title: PIT_P1-PIT_P2-A10-AD006


title: PIT_P7-PIT_P8-G10-AD012


title: PIT_P7-PIT_P8-F4-AD004


title: PIT_P1-PIT_P2-D12-AD010


title: PIT_P1-PIT_P2-C1-AD012


title: PIT_P1-PIT_P2-E10-AD010


title: PIT_P7-PIT_P8-G11-AD010


title: PIT_P7-PIT_P8-G1-AD001


title: PIT_P5-PIT_P6-C11-AD008


title: PIT_P1-PIT_P2-D6-AD002


title: PIT_P3-PIT_P4-A10-AD002


title: PIT_P1-PIT_P2-D8-AD007


title: PIT_P3-PIT_P4-D5-AD006


title: PIT_P3-PIT_P4-F12-AD012


title: PIT_P5-PIT_P6-H2-AD001


title: PIT_P5-PIT_P6-G3-AD007


title: PIT_P1-PIT_P2-A6-AD004


title: PIT_P3-PIT_P4-A11-AD007


title: PIT_P1-PIT_P2-B8-AD006


title: PIT_P1-PIT_P2-C12-AD008


title: PIT_P5-PIT_P6-D10-AD010


title: PIT_P3-PIT_P4-F1-AD001


title: PIT_P5-PIT_P6-G12-AD004


title: PIT_P3-PIT_P4-C12-AD012


title: PIT_P3-PIT_P4-G8-AD008


title: PIT_P3-PIT_P4-E3-AD012


title: PIT_P7-PIT_P8-C7-AD008


title: PIT_P5-PIT_P6-C11-AD004


title: PIT_P3-PIT_P4-F3-AD006


title: PIT_P1-PIT_P2-C9-AD012


title: PIT_P1-PIT_P2-G11-AD012


title: PIT_P5-PIT_P6-D5-AD012


title: PIT_P3-PIT_P4-D12-AD007


title: PIT_P1-PIT_P2-F8-AD006


title: PIT_P7-PIT_P8-D4-AD006


title: PIT_P7-PIT_P8-F3-AD012


title: PIT_P5-PIT_P6-D4-AD010


title: PIT_P7-PIT_P8-F7-AD010


title: PIT_P3-PIT_P4-C1-AD008


title: PIT_P5-PIT_P6-D7-AD004


title: PIT_P3-PIT_P4-H9-AD008


title: PIT_P1-PIT_P2-H2-AD001


title: PIT_P5-PIT_P6-H12-AD008


title: PIT_P5-PIT_P6-H6-AD010


title: PIT_P3-PIT_P4-H9-AD006


title: PIT_P7-PIT_P8-H3-AD006


title: PIT_P5-PIT_P6-D3-AD006


title: PIT_P3-PIT_P4-F11-AD002


title: PIT_P5-PIT_P6-B11-AD004


title: PIT_P1-PIT_P2-B1-AD010


title: PIT_P7-PIT_P8-F2-AD002


title: PIT_P1-PIT_P2-F11-AD007


title: PIT_P7-PIT_P8-G1-AD004


title: PIT_P3-PIT_P4-H3-AD001


title: PIT_P3-PIT_P4-F6-AD010


title: PIT_P7-PIT_P8-H7-AD002


title: PIT_P5-PIT_P6-G1-AD007


title: PIT_P1-PIT_P2-H5-AD012


title: PIT_P3-PIT_P4-G11-AD006


title: PIT_P1-PIT_P2-E11-AD001


title: PIT_P3-PIT_P4-H8-AD001


title: PIT_P1-PIT_P2-F7-AD001


title: PIT_P1-PIT_P2-E6-AD007


title: PIT_P1-PIT_P2-B12-AD004


title: PIT_P5-PIT_P6-E2-AD008


title: PIT_P3-PIT_P4-C11-AD008


title: PIT_P3-PIT_P4-H6-AD008


title: PIT_P7-PIT_P8-G1-AD006


title: PIT_P3-PIT_P4-H3-AD006


title: PIT_P5-PIT_P6-E4-AD010


title: PIT_P3-PIT_P4-A7-AD010


title: PIT_P1-PIT_P2-C9-AD010


title: PIT_P5-PIT_P6-C9-AD008


title: PIT_P3-PIT_P4-G11-AD001


title: PIT_P1-PIT_P2-G7-AD002


title: PIT_P1-PIT_P2-G10-AD012


title: PIT_P3-PIT_P4-C4-AD001


title: PIT_P3-PIT_P4-B4-AD001


title: PIT_P5-PIT_P6-H6-AD006


title: PIT_P1-PIT_P2-A7-AD010


title: PIT_P7-PIT_P8-D9-AD010


title: PIT_P7-PIT_P8-F11-AD012


title: PIT_P1-PIT_P2-B10-AD002


title: PIT_P7-PIT_P8-C8-AD007


title: PIT_P5-PIT_P6-F1-AD007


title: PIT_P5-PIT_P6-A9-AD001


title: PIT_P5-PIT_P6-H11-AD010


title: PIT_P3-PIT_P4-C1-AD007


title: PIT_P1-PIT_P2-F5-AD008


title: PIT_P7-PIT_P8-G4-AD001


title: PIT_P7-PIT_P8-D10-AD008


title: PIT_P1-PIT_P2-D3-AD001


title: PIT_P5-PIT_P6-B7-AD004


title: PIT_P3-PIT_P4-A12-AD006


title: PIT_P5-PIT_P6-A6-AD008


title: PIT_P5-PIT_P6-C5-AD002


title: PIT_P3-PIT_P4-D12-AD010


title: PIT_P1-PIT_P2-C4-AD012


title: PIT_P1-PIT_P2-A12-AD002


title: PIT_P3-PIT_P4-F5-AD007


title: PIT_P3-PIT_P4-C9-AD002


title: PIT_P1-PIT_P2-E9-AD002


title: PIT_P1-PIT_P2-G1-AD002


title: PIT_P3-PIT_P4-F5-AD012


title: PIT_P5-PIT_P6-D6-AD004


title: PIT_P1-PIT_P2-H1-AD006


title: PIT_P3-PIT_P4-G1-AD001


title: PIT_P1-PIT_P2-G8-AD001


title: PIT_P3-PIT_P4-F6-AD012


title: PIT_P1-PIT_P2-B12-AD006


title: PIT_P7-PIT_P8-C1-AD010


title: PIT_P5-PIT_P6-G5-AD001


title: PIT_P5-PIT_P6-B9-AD004


title: PIT_P7-PIT_P8-E7-AD012


title: PIT_P3-PIT_P4-E9-AD012


title: PIT_P7-PIT_P8-E4-AD001


title: PIT_P1-PIT_P2-D10-AD001


title: PIT_P1-PIT_P2-C8-AD010


title: PIT_P7-PIT_P8-A9-AD001


title: PIT_P7-PIT_P8-E4-AD002


title: PIT_P7-PIT_P8-E2-AD008


title: PIT_P5-PIT_P6-G3-AD012


title: PIT_P1-PIT_P2-H3-AD004


title: PIT_P5-PIT_P6-H4-AD004


title: PIT_P3-PIT_P4-H4-AD007


title: PIT_P7-PIT_P8-C4-AD001


title: PIT_P7-PIT_P8-G10-AD002


title: PIT_P5-PIT_P6-A9-AD004


title: PIT_P1-PIT_P2-D2-AD006


title: PIT_P3-PIT_P4-A2-AD006


title: PIT_P1-PIT_P2-F6-AD002


title: PIT_P7-PIT_P8-D12-AD010


title: PIT_P5-PIT_P6-C4-AD012


title: PIT_P1-PIT_P2-D7-AD004


title: PIT_P5-PIT_P6-B12-AD001


title: PIT_P7-PIT_P8-E2-AD001


title: PIT_P7-PIT_P8-H10-AD008


title: PIT_P1-PIT_P2-A7-AD006


title: PIT_P7-PIT_P8-F5-AD007


title: PIT_P5-PIT_P6-A2-AD006


title: PIT_P5-PIT_P6-G7-AD001


title: PIT_P5-PIT_P6-D8-AD006


title: PIT_P5-PIT_P6-B2-AD012


title: PIT_P7-PIT_P8-G7-AD008


title: PIT_P5-PIT_P6-E8-AD010


title: PIT_P5-PIT_P6-D8-AD012


title: PIT_P3-PIT_P4-D3-AD006


title: PIT_P3-PIT_P4-F1-AD002


title: PIT_P7-PIT_P8-C1-AD002


title: PIT_P7-PIT_P8-F8-AD010


title: PIT_P3-PIT_P4-G3-AD008


title: PIT_P5-PIT_P6-G8-AD004


title: PIT_P7-PIT_P8-E12-AD004


title: PIT_P3-PIT_P4-F2-AD008


title: PIT_P7-PIT_P8-C9-AD001


title: PIT_P1-PIT_P2-H12-AD004


title: PIT_P7-PIT_P8-A3-AD012


title: PIT_P3-PIT_P4-B5-AD002


title: PIT_P7-PIT_P8-F3-AD001


title: PIT_P3-PIT_P4-E6-AD010


title: PIT_P5-PIT_P6-E2-AD002


title: PIT_P3-PIT_P4-D2-AD001


title: PIT_P3-PIT_P4-F10-AD006


title: PIT_P3-PIT_P4-E5-AD006


title: PIT_P7-PIT_P8-B12-AD010


title: PIT_P7-PIT_P8-B9-AD001


title: PIT_P1-PIT_P2-B5-AD001


title: PIT_P5-PIT_P6-D8-AD004


title: PIT_P1-PIT_P2-A12-AD008


title: PIT_P7-PIT_P8-F11-AD008


title: PIT_P3-PIT_P4-G3-AD010


title: PIT_P3-PIT_P4-H10-AD002


title: PIT_P3-PIT_P4-A11-AD001


title: PIT_P7-PIT_P8-F6-AD001


title: PIT_P3-PIT_P4-C12-AD004


title: PIT_P1-PIT_P2-D10-AD004


title: PIT_P3-PIT_P4-G3-AD007


title: PIT_P7-PIT_P8-G2-AD006


title: PIT_P7-PIT_P8-H5-AD012


title: PIT_P5-PIT_P6-B3-AD001


title: PIT_P1-PIT_P2-F9-AD006


title: PIT_P3-PIT_P4-C6-AD004


title: PIT_P7-PIT_P8-H6-AD004


title: PIT_P3-PIT_P4-E5-AD004


title: PIT_P3-PIT_P4-F4-AD010


title: PIT_P1-PIT_P2-D3-AD002


title: PIT_P5-PIT_P6-H11-AD004


title: PIT_P3-PIT_P4-C1-AD010


title: PIT_P5-PIT_P6-F1-AD001


title: PIT_P3-PIT_P4-D3-AD007


title: PIT_P7-PIT_P8-C3-AD006


title: PIT_P3-PIT_P4-C10-AD007


title: PIT_P5-PIT_P6-A6-AD012


title: PIT_P7-PIT_P8-F4-AD012


title: PIT_P1-PIT_P2-F3-AD008


title: PIT_P1-PIT_P2-B10-AD007


title: PIT_P7-PIT_P8-E8-AD006


title: PIT_P7-PIT_P8-H4-AD010


title: PIT_P3-PIT_P4-B6-AD008


title: PIT_P3-PIT_P4-E10-AD007


title: PIT_P3-PIT_P4-G7-AD008


title: PIT_P1-PIT_P2-E3-AD006


title: PIT_P5-PIT_P6-H9-AD004


title: PIT_P1-PIT_P2-C1-AD007


title: PIT_P1-PIT_P2-H6-AD010


title: PIT_P7-PIT_P8-G10-AD004


title: PIT_P3-PIT_P4-H9-AD012


title: PIT_P1-PIT_P2-E7-AD008


title: PIT_P1-PIT_P2-H8-AD001


title: PIT_P1-PIT_P2-A12-AD001


title: PIT_P7-PIT_P8-D10-AD004


title: PIT_P5-PIT_P6-F4-AD002


title: PIT_P7-PIT_P8-E7-AD007


title: PIT_P3-PIT_P4-D3-AD002


title: PIT_P5-PIT_P6-D9-AD007


title: PIT_P7-PIT_P8-F2-AD006


title: PIT_P1-PIT_P2-B12-AD012


title: PIT_P7-PIT_P8-G8-AD008


title: PIT_P5-PIT_P6-F10-AD010


title: PIT_P7-PIT_P8-F12-AD012


title: PIT_P5-PIT_P6-H5-AD006


title: PIT_P1-PIT_P2-B10-AD006


title: PIT_P7-PIT_P8-H12-AD006


title: PIT_P5-PIT_P6-D7-AD006


title: PIT_P5-PIT_P6-F4-AD012


title: PIT_P1-PIT_P2-D2-AD001


title: PIT_P3-PIT_P4-A10-AD008


title: PIT_P1-PIT_P2-H7-AD006


title: PIT_P5-PIT_P6-D12-AD004


title: PIT_P3-PIT_P4-H11-AD006


title: PIT_P3-PIT_P4-G10-AD012


title: PIT_P5-PIT_P6-C10-AD008


title: PIT_P3-PIT_P4-H3-AD012


title: PIT_P1-PIT_P2-G12-AD010


title: PIT_P3-PIT_P4-F4-AD006


title: PIT_P1-PIT_P2-E11-AD012


title: PIT_P5-PIT_P6-D4-AD007


title: PIT_P1-PIT_P2-E8-AD004


title: PIT_P7-PIT_P8-H11-AD004


title: PIT_P1-PIT_P2-E1-AD001


title: PIT_P5-PIT_P6-C6-AD002


title: PIT_P5-PIT_P6-E6-AD012


title: PIT_P5-PIT_P6-G7-AD012


title: PIT_P7-PIT_P8-G2-AD001


title: PIT_P3-PIT_P4-G3-AD006


title: PIT_P1-PIT_P2-D12-AD008


title: PIT_P1-PIT_P2-A2-AD007


title: PIT_P7-PIT_P8-G9-AD001


title: PIT_P3-PIT_P4-H8-AD002


title: PIT_P5-PIT_P6-C10-AD012


title: PIT_P1-PIT_P2-G2-AD002


title: PIT_P1-PIT_P2-B7-AD012


title: PIT_P1-PIT_P2-C8-AD007


title: PIT_P7-PIT_P8-H3-AD007


title: PIT_P3-PIT_P4-D8-AD001


title: PIT_P3-PIT_P4-A3-AD012


title: PIT_P3-PIT_P4-D3-AD004


title: PIT_P5-PIT_P6-B1-AD004


title: PIT_P3-PIT_P4-E11-AD010


title: PIT_P1-PIT_P2-D11-AD012


title: PIT_P3-PIT_P4-B6-AD004


title: PIT_P3-PIT_P4-E12-AD001


title: PIT_P5-PIT_P6-F7-AD001


title: PIT_P5-PIT_P6-A4-AD002


title: PIT_P7-PIT_P8-B4-AD008


title: PIT_P7-PIT_P8-D11-AD001


title: PIT_P3-PIT_P4-H2-AD007


title: PIT_P5-PIT_P6-H11-AD012


title: PIT_P3-PIT_P4-C6-AD002


title: PIT_P3-PIT_P4-D11-AD010


title: PIT_P5-PIT_P6-C8-AD001


title: PIT_P1-PIT_P2-A9-AD007


title: PIT_P5-PIT_P6-F5-AD006


title: PIT_P5-PIT_P6-H3-AD004


title: PIT_P7-PIT_P8-B6-AD001


title: PIT_P5-PIT_P6-F5-AD008


title: PIT_P1-PIT_P2-G12-AD002


title: PIT_P3-PIT_P4-F1-AD006


title: PIT_P5-PIT_P6-G1-AD010


title: PIT_P5-PIT_P6-E9-AD004


title: PIT_P5-PIT_P6-H4-AD002


title: PIT_P7-PIT_P8-H11-AD006


title: PIT_P7-PIT_P8-D2-AD007


title: PIT_P7-PIT_P8-A4-AD008


title: PIT_P3-PIT_P4-G11-AD002


title: PIT_P3-PIT_P4-D6-AD007


title: PIT_P7-PIT_P8-H11-AD010


title: PIT_P1-PIT_P2-F7-AD006


title: PIT_P3-PIT_P4-G7-AD002


title: PIT_P5-PIT_P6-F7-AD004


title: PIT_P5-PIT_P6-G4-AD012


title: PIT_P1-PIT_P2-C6-AD006


title: PIT_P1-PIT_P2-F3-AD004


title: PIT_P3-PIT_P4-D11-AD007


title: PIT_P3-PIT_P4-G12-AD008


title: PIT_P5-PIT_P6-C7-AD007


title: PIT_P3-PIT_P4-E7-AD001


title: PIT_P7-PIT_P8-G7-AD006


title: PIT_P3-PIT_P4-A8-AD001


title: PIT_P3-PIT_P4-C3-AD006


title: PIT_P5-PIT_P6-C5-AD012


title: PIT_P5-PIT_P6-B12-AD004


title: PIT_P3-PIT_P4-E1-AD010


title: PIT_P3-PIT_P4-F1-AD008


title: PIT_P7-PIT_P8-E3-AD010


title: PIT_P3-PIT_P4-F10-AD007


title: PIT_P1-PIT_P2-D5-AD007


title: PIT_P3-PIT_P4-G12-AD002


title: PIT_P3-PIT_P4-D7-AD010


title: PIT_P3-PIT_P4-H7-AD008


title: PIT_P3-PIT_P4-B1-AD012


title: PIT_P3-PIT_P4-D8-AD004


title: PIT_P5-PIT_P6-A8-AD002


title: PIT_P1-PIT_P2-E7-AD001


title: PIT_P3-PIT_P4-G5-AD007


title: PIT_P5-PIT_P6-B10-AD012


title: PIT_P7-PIT_P8-D2-AD012


title: PIT_P3-PIT_P4-F6-AD006


title: PIT_P7-PIT_P8-E5-AD010


title: PIT_P5-PIT_P6-D6-AD002


title: PIT_P3-PIT_P4-E12-AD004


title: PIT_P1-PIT_P2-H11-AD012


title: PIT_P7-PIT_P8-G2-AD010


title: PIT_P1-PIT_P2-A2-AD010


title: PIT_P7-PIT_P8-B8-AD008


title: PIT_P7-PIT_P8-E5-AD004


title: PIT_P5-PIT_P6-H4-AD006


title: PIT_P3-PIT_P4-H10-AD007


title: PIT_P1-PIT_P2-E5-AD004


title: PIT_P7-PIT_P8-C3-AD001


title: PIT_P1-PIT_P2-A7-AD004


title: PIT_P5-PIT_P6-B5-AD006


title: PIT_P5-PIT_P6-D4-AD001


title: PIT_P7-PIT_P8-F9-AD007


title: PIT_P1-PIT_P2-A5-AD002


title: PIT_P5-PIT_P6-F9-AD007


title: PIT_P1-PIT_P2-D4-AD012


title: PIT_P5-PIT_P6-E1-AD008


title: PIT_P3-PIT_P4-C2-AD001


title: PIT_P1-PIT_P2-H8-AD007


title: PIT_P5-PIT_P6-D9-AD001


title: PIT_P7-PIT_P8-H11-AD002


title: PIT_P5-PIT_P6-H1-AD012