title: m.NJD00101.P09


title: m.NJD00101.O22

status: Public on May 20 2021

type: SRA

city: Stockholm

first: Martin

last: Enge

address: CCK, Z4

department: Dep of Oncology-Pathology

country: Sweden

institute: Karolinska Institute

library_source: transcriptomic

data_processing:
Sequencing reads were trimmed, adapter sequences removed
Reads aligned to the mm10 reference assembly using STAR with default parameters
Duplicate reads were removed using picard
Transcript counts were obtained using HTSeq35 and mm10 UCSC exon/transcript annotations
Single cell profiles with the following features were deemed to be of poor quality and removed: 1) cells with less than a specified total number of valid counts in exonic regions (1.8x104 and 4x104 for the mouse gut and sorted multiplets dataset, respectively), 2) cells with low actin TPM, and 3) cells with high fractions of ERCC reads. To determine a cut-off for actin TPM, we used the normal distribution with empirical mean and standard deviation from log2 transformed actin TPM, which is normally distributed in successful experiments. The cut-off was set to the 0.01 quantile (eg. the lower 0.01 % of the bell curve). A similar strategy was used for fractions of ERCC reads, samples with a log2 transformed fraction of ERCC reads above the 0.99 % quantile were rejected.
Genome_build: mm10 (GRCm38)
Supplementary_files_format_and_content: htseq-format of raw counts

molecule: total RNA

organism: Mus musculus

source_name: colon

growth_protocol: Small intestine and colon were removed from C57BL/J wild-type mice and kept on ice in phosphate buffered saline (PBS). Lumina of colon and small intestine were washed three times with PBS and adipose tissue connected to the exterior of the small intestine or colon was removed. Colon and small intestine were opened longitudinally, and any remaining mucous on covering the epithelial layer was gently rubbed off. Tissues were washed once in PBS before being cut into 0.5 - 1 mm long fragments. Colon fragments were immersed in 10mM EDTA-PBS and incubated for 105 minutes on ice, with shaking every 15 minutes. Small intestinal fragments were similarly immersed in 10mM EDTA-PBS on ice and shaken. After 15 minutes, small intestinal fragments were allowed to settle at the bottom and the supernatant was discarded and replaced with new 10mM EDTA-PBS and shaken vigorously. This procedure was repeated 1-2 times, with supernatant fractions investigated through light microscopy before discarding, in order to enrich for crypts. Small intestinal fragments were then incubated on ice for 45-60 minutes for a total of 105 minutes. Following EDTA-PBS treatment fractions were triturated 10-15 times. Colon fractions were strained through a 100 um filter while small intestinal fractions were strained through a 70 um filter. Fractions were centrifuged at 300g for 5 minutes and dissociated using TrypLE Express (Invitrogen) at 37°C for 15-20 minutes. Enzymatic dissociation was supervised using light microscopy during regular intervals to obtain an appropriate amount of single cells and multiplets. Dead cells were removed using Dead Cell Removal Kit (Miltenyi Biotec) according to the manufacturer's protocol before staining for FACS sorting was performed.

extract_protocol:
For mouse intestine, cells were stained with FITC anti-CD326 and 7AAD (Sony Biotechnology) in order to monitor number of epithelial cells and select live cells. Additionally, paneth cells were enriched using Brilliant Violent anti-CD24. For spleen, cells were sorted with only 7AAD to remove dead cells, not other antibodies. Single cells were distinguished from multiplets using FSC-H and FSC-W gating and the gating scheme was visually confirmed via phase contrast microscopy (Fig. 1a). Cells were sorted into 384- or 96-well plates containing hypotonic lysis buffer using a SH800S FACS sorter (Sony). Plates were sealed immediately after sorting with microseal F foil (Biorad) and centrifuged at 4000g at 4°C for 5 min before being frozen on dry ice and stored at -80°C.
Single-cell and multiplet RNA-Seq libraries were generated as described. Briefly, single-cells collected in 384-well plates were lysed, followed by reverse transcription with template-switch using an LNA-modified template switch oligo to generate cDNA. After 21 cycles of pre-amplification, DNA was purified and analysed on an automated Fragment Analyzer (Advanced Analytical). Each cell’s cDNA fragment profile was individually inspected and only wells with successful amplification products (concentration higher than 0.06 ng/ul) and with no detectable RNA degradation were selected for final library preparation. Tagmentation assays and barcoded sequencing libraries were prepared using Nextera XT kit (Illumina) according to the manufacturer's instructions. Barcoded libraries were pooled and subjected to 75 bp paired-end sequencing on the Illumina NextSeq 500 instrument.

tag: strain

tag: tissue

value: colon

tag: facs cellnumber

value: Multiplet

tag: age (days)


title: m.NJD00101.O15


title: m.NJD00101.P03


title: m.NJD00101.O17


title: m.NJD00101.O21


title: m.NJD00101.O20


title: m.NJD00101.O09


title: m.NJD00101.O08


title: m.NJD00101.P16


title: m.NJD00101.O07


title: m.NJD00101.O06


title: m.NJD00101.O03


title: m.NJD00101.O05


title: m.NJD00101.O02


title: m.NJD00101.N24


title: m.NJD00101.O04


title: m.NJD00101.N22


title: m.NJD00101.N13


title: m.NJD00101.N09


title: m.NJD00101.N10


title: m.NJD00101.N08


title: m.NJD00101.N21


title: m.NJD00101.N11


title: m.NJD00101.N16


title: m.NJD00101.N04


title: m.NJD00101.N06


title: m.NJD00101.N03


title: m.NJD00101.N23


title: m.NJD00101.N02


title: m.NJD00101.N19


title: m.NJD00101.M23


title: m.NJD00101.N07


title: m.NJD00101.M22


title: m.NJD00101.M21


title: m.NJD00101.N14


title: m.NJD00101.M24


title: m.NJD00101.M20


title: m.NJD00101.N18


title: m.NJD00101.N20


title: m.NJD00101.M19


title: m.NJD00101.N05


title: m.NJD00101.M17


title: m.NJD00101.M16


title: m.NJD00101.M13


title: m.NJD00101.M11


title: m.NJD00101.M09


title: m.NJD00101.M08


title: m.NJD00101.L24


title: m.NJD00101.M01


title: m.NJD00101.L21


title: m.NJD00101.L19


title: m.NJD00101.L20


title: m.NJD00101.M15


title: m.NJD00101.M06


title: m.NJD00101.L14


title: m.NJD00101.L13


title: m.NJD00101.M07


title: m.NJD00101.L17


title: m.NJD00101.L16


title: m.NJD00101.L15


title: m.NJD00101.L11


title: m.NJD00101.L10


title: m.NJD00101.L12


title: m.NJD00101.L08


title: m.NJD00101.L09


title: m.NJD00101.L06


title: m.NJD00101.L04


title: m.NJD00101.M14


title: m.NJD00101.L23


title: m.NJD00101.L18


title: m.NJD00101.L01


title: m.NJD00101.L07


title: m.NJD00101.L03


title: m.NJD00101.L22


title: m.NJD00101.K24


title: m.NJD00101.K14


title: m.NJD00101.K13


title: m.NJD00101.K20


title: m.NJD00101.K18


title: m.NJD00101.K17


title: m.NJD00101.K16


title: m.NJD00101.K19


title: m.NJD00101.K10


title: m.NJD00101.K12


title: m.NJD00101.K06


title: m.NJD00101.K07


title: m.NJD00101.K08


title: m.NJD00101.L05


title: m.NJD00101.K04


title: m.NJD00101.K05


title: m.NJD00101.K23


title: m.NJD00101.J21


title: m.NJD00101.K01


title: m.NJD00101.J22


title: m.NJD00101.K03


title: m.NJD00101.J24


title: m.NJD00101.J23


title: m.NJD00101.K22


title: m.NJD00101.J20


title: m.NJD00101.J18


title: m.NJD00101.J19


title: m.NJD00101.J17


title: m.NJD00101.K09


title: m.NJD00101.J05


title: m.NJD00101.I24


title: m.NJD00101.J09


title: m.NJD00101.J15


title: m.NJD00101.J06


title: m.NJD00101.J11


title: m.NJD00101.J14


title: m.NJD00101.J08


title: m.NJD00101.J07


title: m.NJD00101.J02


title: m.NJD00101.J10


title: m.NJD00101.J04


title: m.NJD00101.J16


title: m.NJD00101.J13


title: m.NJD00101.J03


title: m.NJD00101.I22


title: m.NJD00101.I19


title: m.NJD00101.I21


title: m.NJD00101.I20


title: m.NJD00101.I18


title: m.NJD00101.I15


title: m.NJD00101.I16


title: m.NJD00101.I17


title: m.NJD00101.J12


title: m.NJD00101.J01


title: m.NJD00101.I10


title: m.NJD00101.I14


title: m.NJD00101.I12


title: m.NJD00101.I13


title: m.NJD00101.I05


title: m.NJD00101.I04


title: m.NJD00101.I02


title: m.NJD00101.I07


title: m.NJD00101.H24


title: m.NJD00101.I03


title: m.NJD00101.I01


title: m.NJD00101.H18


title: m.NJD00101.H16


title: m.NJD00101.H10


title: m.NJD00101.I09


title: m.NJD00101.H13


title: m.NJD00101.H14


title: m.NJD00101.H21


title: m.NJD00101.I11


title: m.NJD00101.H20


title: m.NJD00101.H23


title: m.NJD00101.H19


title: m.NJD00101.I08


title: m.NJD00101.H11


title: m.NJD00101.H12


title: m.NJD00101.H17


title: m.NJD00101.H07


title: m.NJD00101.H15


title: m.NJD00101.H09


title: m.NJD00101.H05


title: m.NJD00101.I06


title: m.NJD00101.H06


title: m.NJD00101.H04


title: m.NJD00101.H03


title: m.NJD00101.H08


title: m.NJD00101.G24


title: m.NJD00101.G12


title: m.NJD00101.G23


title: m.NJD00101.G14


title: m.NJD00101.G19


title: m.NJD00101.G20


title: m.NJD00101.G16


title: m.NJD00101.G13


title: m.NJD00101.G11


title: m.NJD00101.G21


title: m.NJD00101.G15


title: m.NJD00101.H02


title: m.NJD00101.H01


title: m.NJD00101.G18


title: m.NJD00101.G09


title: m.NJD00101.G17


title: m.NJD00101.G02


title: m.NJD00101.G06


title: m.NJD00101.G05


title: m.NJD00101.G07


title: m.NJD00101.G10


title: m.NJD00101.G01


title: m.NJD00101.F24


title: m.NJD00101.F22


title: m.NJD00101.F21


title: m.NJD00101.G22


title: m.NJD00101.G08


title: m.NJD00101.F19


title: m.NJD00101.F18


title: m.NJD00101.F17


title: m.NJD00101.F16


title: m.NJD00101.F12


title: m.NJD00101.F13


title: m.NJD00101.F15


title: m.NJD00101.F06


title: m.NJD00101.F08


title: m.NJD00101.F07


title: m.NJD00101.E24


title: m.NJD00101.F02


title: m.NJD00101.E23


title: m.NJD00101.F11


title: m.NJD00101.E22


title: m.NJD00101.E19


title: m.NJD00101.E20


title: m.NJD00101.F04


title: m.NJD00101.F10


title: m.NJD00101.E21


title: m.NJD00101.F20


title: m.NJD00101.E14


title: m.NJD00101.E15


title: m.NJD00101.E13


title: m.NJD00101.E12


title: m.NJD00101.F14


title: m.NJD00101.E10


title: m.NJD00101.F09


title: m.NJD00101.E11


title: m.NJD00101.F05


title: m.NJD00101.E08


title: m.NJD00101.F03


title: m.NJD00101.E07


title: m.NJD00101.E03


title: m.NJD00101.D21


title: m.NJD00101.D18


title: m.NJD00101.D17


title: m.NJD00101.D19


title: m.NJD00101.D16


title: m.NJD00101.D23


title: m.NJD00101.E04


title: m.NJD00101.D14


title: m.NJD00101.E18


title: m.NJD00101.D13


title: m.NJD00101.D12


title: m.NJD00101.D11


title: m.NJD00101.D15


title: m.NJD00101.D10


title: m.NJD00101.D09


title: m.NJD00101.D08


title: m.NJD00101.D07


title: m.NJD00101.E09


title: m.NJD00101.D05


title: m.NJD00101.D06


title: m.NJD00101.D03


title: m.NJD00101.D04


title: m.NJD00101.D02


title: m.NJD00101.D01


title: m.NJD00101.D24


title: m.NJD00101.E01


title: m.NJD00101.C24


title: m.NJD00101.C20


title: m.NJD00101.D20


title: m.NJD00101.C19


title: m.NJD00101.C18


title: m.NJD00101.C15


title: m.NJD00101.C12


title: m.NJD00101.C14


title: m.NJD00101.C16


title: m.NJD00101.C13


title: m.NJD00101.C11


title: m.NJD00101.C17


title: m.NJD00101.C10


title: m.NJD00101.C09


title: m.NJD00101.C04


title: m.NJD00101.C07


title: m.NJD00101.C02


title: m.NJD00101.C03


title: m.NJD00101.C01


title: m.NJD00101.B24


title: m.NJD00101.B22


title: m.NJD00101.B23


title: m.NJD00101.B21


title: m.NJD00101.B20


title: m.NJD00101.C22


title: m.NJD00101.B18


title: m.NJD00101.B17


title: m.NJD00101.B16


title: m.NJD00101.B12


title: m.NJD00101.B13


title: m.NJD00101.B14


title: m.NJD00101.C05


title: m.NJD00101.B03


title: m.NJD00101.B15


title: m.NJD00101.A24


title: m.NJD00101.B01


title: m.NJD00101.B08


title: m.NJD00101.B06


title: m.NJD00101.A19


title: m.NJD00101.B07


title: m.NJD00101.A20


title: m.NJD00101.B02


title: m.NJD00101.A18


title: m.NJD00101.B05


title: m.NJD00101.A21


title: m.NJD00101.B04


title: m.NJD00101.A22


title: m.NJD00101.A17


title: m.NJD00101.A16


title: m.NJD00101.A12


title: m.NJD00101.A13


title: m.NJD00101.A14


title: m.NJD00101.A09


title: m.NJD00101.A10


title: m.NJD00101.A08


title: m.NJD00101.A07


title: m.NJD00101.A06


title: m.NJD00101.A05


title: m.NJD00101.A03


title: m.NJD00101.A02


title: m.NJD00102.P23


title: m.NJD00102.P22


title: m.NJD00102.P20


title: m.NJD00102.P24


title: m.NJD00102.P16


title: m.NJD00101.A01


title: m.NJD00102.P19


title: m.NJD00102.P13


title: m.NJD00102.P21


title: m.NJD00102.P14


title: m.NJD00102.P18


title: m.NJD00102.P10


title: m.NJD00102.P11


title: m.NJD00102.P09


title: m.NJD00102.P17


title: m.NJD00102.P12


title: m.NJD00102.P08


title: m.NJD00102.P05


title: m.NJD00102.P06


title: m.NJD00102.P07


title: m.NJD00102.P03


title: m.NJD00102.O24


title: m.NJD00102.P04


title: m.NJD00101.A11


title: m.NJD00102.P02


title: m.NJD00102.O23


title: m.NJD00102.O22


title: m.NJD00102.O19


title: m.NJD00102.O20


title: m.NJD00102.O18


title: m.NJD00102.O14


title: m.NJD00102.O17


title: m.NJD00102.O16


title: m.NJD00102.O15


title: m.NJD00102.O12


title: m.NJD00102.O13


title: m.NJD00102.O10


title: m.NJD00102.O04


title: m.NJD00102.O11


title: m.NJD00102.O07


title: m.NJD00102.O08


title: m.NJD00102.O09


title: m.NJD00102.O01


title: m.NJD00102.O05


title: m.NJD00102.N21


title: m.NJD00102.N20


title: m.NJD00102.N19


title: m.NJD00102.N18


title: m.NJD00102.N15


title: m.NJD00102.N23


title: m.NJD00102.N24


title: m.NJD00102.N14


title: m.NJD00102.N13


title: m.NJD00102.N10


title: m.NJD00102.N16


title: m.NJD00102.N09


title: m.NJD00102.O06


title: m.NJD00102.N08


title: m.NJD00102.O03


title: m.NJD00102.N07


title: m.NJD00102.N05


title: m.NJD00102.N12


title: m.NJD00102.O02


title: m.NJD00102.N11


title: m.NJD00102.N03


title: m.NJD00102.N02


title: m.NJD00102.N01


title: m.NJD00102.M20


title: m.NJD00102.M16


title: m.NJD00102.M18


title: m.NJD00102.M24


title: m.NJD00102.M14


title: m.NJD00102.M12


title: m.NJD00102.M11


title: m.NJD00102.M15


title: m.NJD00102.M09


title: m.NJD00102.M07


title: m.NJD00102.M06


title: m.NJD00102.M02


title: m.NJD00102.N06


title: m.NJD00102.M01


title: m.NJD00102.L22


title: m.NJD00102.L21


title: m.NJD00102.N04


title: m.NJD00102.L24


title: m.NJD00102.L20


title: m.NJD00102.M22


title: m.NJD00102.L17


title: m.NJD00102.L14


title: m.NJD00102.L13


title: m.NJD00102.L15


title: m.NJD00102.L11


title: m.NJD00102.L12


title: m.NJD00102.M03


title: m.NJD00102.L08


title: m.NJD00102.L07


title: m.NJD00102.L10


title: m.NJD00102.L04


title: m.NJD00102.L02


title: m.NJD00102.L05


title: m.NJD00102.L03


title: m.NJD00102.L01


title: m.NJD00102.K23


title: m.NJD00102.L23


title: m.NJD00102.K24


title: m.NJD00102.K22


title: m.NJD00102.K21


title: m.NJD00102.L16


title: m.NJD00102.L19


title: m.NJD00102.K16


title: m.NJD00102.K17


title: m.NJD00102.K15


title: m.NJD00102.K18


title: m.NJD00102.K20


title: m.NJD00102.K14


title: m.NJD00102.K12


title: m.NJD00102.K09


title: m.NJD00102.L09


title: m.NJD00102.K08


title: m.NJD00102.K06


title: m.NJD00102.K13


title: m.NJD00102.K03


title: m.NJD00102.J23


title: m.NJD00102.J24


title: m.NJD00102.K01


title: m.NJD00102.J22


title: m.NJD00102.J21


title: m.NJD00102.K04


title: m.NJD00102.K02


title: m.NJD00102.J18


title: m.NJD00102.J16


title: m.NJD00102.J20


title: m.NJD00102.J17


title: m.NJD00102.J15


title: m.NJD00102.J13


title: m.NJD00102.J14


title: m.NJD00102.J11


title: m.NJD00102.J08


title: m.NJD00102.J07


title: m.NJD00102.K07


title: m.NJD00102.J10


title: m.NJD00102.I24


title: m.NJD00102.J06


title: m.NJD00102.I23


title: m.NJD00102.J05


title: m.NJD00102.J01


title: m.NJD00102.J03


title: m.NJD00102.I20


title: m.NJD00102.J04


title: m.NJD00102.I15


title: m.NJD00102.I16


title: m.NJD00102.I14


title: m.NJD00102.I12


title: m.NJD00102.I18


title: m.NJD00102.I10


title: m.NJD00102.I11


title: m.NJD00102.I17


title: m.NJD00102.I07


title: m.NJD00102.I06


title: m.NJD00102.I04


title: m.NJD00102.I08


title: m.NJD00102.I01


title: m.NJD00102.I03


title: m.NJD00102.I02


title: m.NJD00102.H23


title: m.NJD00102.I22


title: m.NJD00102.I19


title: m.NJD00102.H22


title: m.NJD00102.H21


title: m.NJD00102.H17


title: m.NJD00102.H19


title: m.NJD00102.H15


title: m.NJD00102.H20


title: m.NJD00102.H18


title: m.NJD00102.H14


title: m.NJD00102.H13


title: m.NJD00102.I09


title: m.NJD00102.H11


title: m.NJD00102.H07


title: m.NJD00102.H12


title: m.NJD00102.H10


title: m.NJD00102.H06


title: m.NJD00102.H09


title: m.NJD00102.H02


title: m.NJD00102.H05


title: m.NJD00102.H03


title: m.NJD00102.G24


title: m.NJD00102.H01


title: m.NJD00102.G23


title: m.NJD00102.G22


title: m.NJD00102.G21


title: m.NJD00102.G20


title: m.NJD00102.H16


title: m.NJD00102.G19


title: m.NJD00102.G18


title: m.NJD00102.G17


title: m.NJD00102.G16


title: m.NJD00102.G10


title: m.NJD00102.G12


title: m.NJD00102.G14


title: m.NJD00102.G11


title: m.NJD00102.H08


title: m.NJD00102.G09


title: m.NJD00102.G07


title: m.NJD00102.G06


title: m.NJD00102.G04


title: m.NJD00102.F22


title: m.NJD00102.F24


title: m.NJD00102.G02


title: m.NJD00102.F19


title: m.NJD00102.F14


title: m.NJD00102.F11


title: m.NJD00102.F13


title: m.NJD00102.F18


title: m.NJD00102.F12


title: m.NJD00102.F16


title: m.NJD00102.F07


title: m.NJD00102.F09


title: m.NJD00102.F08


title: m.NJD00102.F10


title: m.NJD00102.G01


title: m.NJD00102.E20


title: m.NJD00102.F06


title: m.NJD00102.F02


title: m.NJD00102.E22


title: m.NJD00102.E21


title: m.NJD00102.F04


title: m.NJD00102.E19


title: m.NJD00102.E23


title: m.NJD00102.F15


title: m.NJD00102.F20


title: m.NJD00102.F17


title: m.NJD00102.F03


title: m.NJD00102.E18


title: m.NJD00102.E16


title: m.NJD00102.E14


title: m.NJD00102.E12


title: m.NJD00102.E07


title: m.NJD00102.E13


title: m.NJD00102.E03


title: m.NJD00102.E04


title: m.NJD00102.E08


title: m.NJD00102.E06


title: m.NJD00102.E05


title: m.NJD00102.E02


title: m.NJD00102.E15


title: m.NJD00102.E24


title: m.NJD00102.D23


title: m.NJD00102.D20


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title: m.NJD00102.E17


title: m.NJD00102.D18


title: m.NJD00102.D19


title: m.NJD00102.D22


title: m.NJD00102.D17


title: m.NJD00102.D24


title: m.NJD00102.D15


title: m.NJD00102.D16


title: m.NJD00102.D13


title: m.NJD00102.D12


title: m.NJD00102.D14


title: m.NJD00102.E11


title: m.NJD00102.D10


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title: m.NJD00102.D09


title: m.NJD00102.D07


title: m.NJD00102.K10


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title: m.NJD00102.D04


title: m.NJD00102.D06


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title: m.NJD00102.D02


title: m.NJD00102.C22


title: m.NJD00102.D11


title: m.NJD00102.C24


title: m.NJD00102.C18


title: m.NJD00102.C14


title: m.NJD00102.C12


title: m.NJD00102.C21


title: m.NJD00102.C23


title: m.NJD00102.C16


title: m.NJD00102.C20


title: m.NJD00102.C09


title: m.NJD00102.C10


title: m.NJD00102.C07


title: m.NJD00102.C15


title: m.NJD00102.C17


title: m.NJD00102.C11


title: m.NJD00102.C06


title: m.NJD00102.C08


title: m.NJD00102.C05


title: m.NJD00102.C04


title: m.NJD00102.C03


title: m.NJD00102.C02


title: m.NJD00102.C01


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title: m.NJD00102.B23


title: m.NJD00102.B24


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title: m.NJD00102.B21


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title: m.NJD00102.B17


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title: m.NJD00102.B16


title: m.NJD00102.B15


title: m.NJD00102.B13


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title: m.NJD00102.B11


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title: m.NJD00102.B06


title: m.NJD00102.A24


title: m.NJD00102.A19


title: m.NJD00102.A20


title: m.NJD00102.B01


title: m.NJD00102.A17


title: m.NJD00102.A18


title: m.NJD00102.A13


title: m.NJD00102.A16


title: m.NJD00102.A14


title: m.NJD00102.A11


title: m.NJD00102.A12


title: m.NJD00102.A10


title: m.NJD00102.A09


title: m.NJD00102.A07


title: m.NJD00102.A08


title: m.NJD00102.A05


title: m.NJD00102.A03


title: m.NJD00103.P24


title: m.NJD00102.A04


title: m.NJD00103.P20


title: m.NJD00103.P19


title: m.NJD00102.A02


title: m.NJD00103.P23


title: m.NJD00103.P22


title: m.NJD00102.A15


title: m.NJD00103.P17


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title: m.NJD00103.P18


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title: m.NJD00103.P16


title: m.NJD00103.P09


title: m.NJD00103.P13


title: m.NJD00103.P12


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title: m.NJD00103.P15


title: m.NJD00103.P10


title: m.NJD00103.P11


title: m.NJD00103.P05


title: m.NJD00103.O23


title: m.NJD00103.O22


title: m.NJD00103.O16


title: m.NJD00103.O21


title: m.NJD00103.O19


title: m.NJD00103.P02


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title: m.NJD00103.O12


title: m.NJD00103.O11


title: m.NJD00103.O10


title: m.NJD00103.O24


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title: m.NJD00103.O08


title: m.NJD00103.O18


title: m.NJD00103.O17


title: m.NJD00103.O15


title: m.NJD00103.O06


title: m.NJD00103.O01


title: m.NJD00103.O05


title: m.NJD00103.O03


title: m.NJD00103.N21


title: m.NJD00103.N24


title: m.NJD00103.N22


title: m.NJD00103.N20


title: m.NJD00103.O04


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title: m.NJD00103.O20


title: m.NJD00103.O13


title: m.NJD00103.N18


title: m.NJD00103.N17


title: m.NJD00103.N14


title: m.NJD00103.N15


title: m.NJD00103.N13


title: m.NJD00103.N12


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title: m.NJD00103.N06


title: m.NJD00103.N07


title: m.NJD00103.N04


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title: m.NJD00103.N01


title: m.NJD00103.O02


title: m.NJD00103.M23


title: m.NJD00103.N16


title: m.NJD00103.M15


title: m.NJD00103.M17


title: m.NJD00103.M13


title: m.NJD00103.M14


title: m.NJD00103.M21


title: m.NJD00103.M11


title: m.NJD00103.N10


title: m.NJD00103.M16


title: m.NJD00103.M12


title: m.NJD00103.N08


title: m.NJD00103.M10


title: m.NJD00103.M08


title: m.NJD00103.M05


title: m.NJD00103.M07


title: m.NJD00103.M06


title: m.NJD00103.N09


title: m.NJD00103.M03


title: m.NJD00103.M01


title: m.NJD00103.L23


title: m.NJD00103.L24


title: m.NJD00103.L21


title: m.NJD00103.L20


title: m.NJD00103.M24


title: m.NJD00103.L19


title: m.NJD00103.M20


title: m.NJD00103.M18


title: m.NJD00103.M22


title: m.NJD00103.M09


title: m.NJD00103.L17


title: m.NJD00103.L16


title: m.NJD00103.L15


title: m.NJD00103.L13


title: m.NJD00103.L08


title: m.NJD00103.L10


title: m.NJD00103.L04


title: m.NJD00103.K24


title: m.NJD00103.L05


title: m.NJD00103.L07


title: m.NJD00103.L03


title: m.NJD00103.L01


title: m.NJD00103.K23


title: m.NJD00103.L09


title: m.NJD00103.L12


title: m.NJD00103.L11


title: m.NJD00103.L02


title: m.NJD00103.K19


title: m.NJD00103.K20


title: m.NJD00103.K18


title: m.NJD00103.K15


title: m.NJD00103.K16


title: m.NJD00103.K12


title: m.NJD00103.K14


title: m.NJD00103.K13


title: m.NJD00103.K11


title: m.NJD00103.K08


title: m.NJD00103.K09


title: m.NJD00103.K07


title: m.NJD00103.K06


title: m.NJD00103.K05


title: m.NJD00103.K04


title: m.NJD00103.K17


title: m.NJD00103.K03


title: m.NJD00103.K01


title: m.NJD00103.J24


title: m.NJD00103.J23


title: m.NJD00103.J18


title: m.NJD00103.J20


title: m.NJD00103.J16


title: m.NJD00103.J17


title: m.NJD00103.J10


title: m.NJD00103.J13


title: m.NJD00103.J14


title: m.NJD00103.J09


title: m.NJD00103.J11


title: m.NJD00103.J04


title: m.NJD00103.J07


title: m.NJD00103.J03


title: m.NJD00103.I24


title: m.NJD00103.J08


title: m.NJD00103.J22


title: m.NJD00103.I23


title: m.NJD00103.I22


title: m.NJD00103.J06


title: m.NJD00103.I21


title: m.NJD00103.I20


title: m.NJD00103.I18


title: m.NJD00103.I16


title: m.NJD00103.I17


title: m.NJD00103.J19


title: m.NJD00103.I13


title: m.NJD00103.J12


title: m.NJD00103.I19


title: m.NJD00103.I11


title: m.NJD00103.I09


title: m.NJD00103.I05


title: m.NJD00103.H09


title: m.NJD00103.H13


title: m.NJD00103.H20


title: m.NJD00103.H16


title: m.NJD00103.H23


title: m.NJD00103.I07


title: m.NJD00103.I12


title: m.NJD00103.I10


title: m.NJD00103.H18


title: m.NJD00103.H10


title: m.NJD00103.I15


title: m.NJD00103.H12


title: m.NJD00103.H17


title: m.NJD00103.H15


title: m.NJD00103.I04


title: m.NJD00103.H14


title: m.NJD00103.I02


title: m.NJD00103.H19


title: m.NJD00103.I03


title: m.NJD00103.H06


title: m.NJD00103.H07


title: m.NJD00103.H03


title: m.NJD00103.H05


title: m.NJD00103.H04


title: m.NJD00103.H02


title: m.NJD00103.H21


title: m.NJD00103.H01


title: m.NJD00103.G24


title: m.NJD00103.G23


title: m.NJD00103.G22


title: m.NJD00103.G21


title: m.NJD00103.G18


title: m.NJD00103.G15


title: m.NJD00103.G19


title: m.NJD00103.G16


title: m.NJD00103.G14


title: m.NJD00103.G13


title: m.NJD00103.G10


title: m.NJD00103.G07


title: m.NJD00103.G06


title: m.NJD00103.G12


title: m.NJD00103.G11


title: m.NJD00103.G04


title: m.NJD00103.G05


title: m.NJD00103.G01


title: m.NJD00103.F20


title: m.NJD00103.F22


title: m.NJD00103.F24


title: m.NJD00103.F18


title: m.NJD00103.F19


title: m.NJD00103.F17


title: m.NJD00103.F16


title: m.NJD00103.F15


title: m.NJD00103.F14


title: m.NJD00103.G17


title: m.NJD00103.F13


title: m.NJD00103.G08


title: m.NJD00103.F12


title: m.NJD00103.F10


title: m.NJD00103.G03


title: m.NJD00103.G02


title: m.NJD00103.F11


title: m.NJD00103.F06


title: m.NJD00103.F05


title: m.NJD00103.F07


title: m.NJD00103.E22


title: m.NJD00103.F02


title: m.NJD00103.F03


title: m.NJD00103.F01


title: m.NJD00103.E20


title: m.NJD00103.E21


title: m.NJD00103.E19


title: m.NJD00103.E18


title: m.NJD00103.E17


title: m.NJD00103.E12


title: m.NJD00103.D23


title: m.NJD00103.E09


title: m.NJD00103.D24


title: m.NJD00103.E11


title: m.NJD00103.D21


title: m.NJD00103.E03


title: m.NJD00103.E02


title: m.NJD00103.D19


title: m.NJD00103.E24


title: m.NJD00103.E23


title: m.NJD00103.F08


title: m.NJD00103.D14


title: m.NJD00103.D12


title: m.NJD00103.D13


title: m.NJD00103.E15


title: m.NJD00103.E01


title: m.NJD00103.E08


title: m.NJD00103.E14


title: m.NJD00103.D20


title: m.NJD00103.E07


title: m.NJD00103.E10


title: m.NJD00103.E05


title: m.NJD00103.D18


title: m.NJD00103.D10


title: m.NJD00103.D09


title: m.NJD00103.E13


title: m.NJD00103.D22


title: m.NJD00103.D17


title: m.NJD00103.D08


title: m.NJD00103.D06


title: m.NJD00103.C14


title: m.NJD00103.C22


title: m.NJD00103.C03


title: m.NJD00103.C05


title: m.NJD00103.C11


title: m.NJD00103.C19


title: m.NJD00103.C12


title: m.NJD00103.C08


title: m.NJD00103.C10


title: m.NJD00103.C24


title: m.NJD00103.C17


title: m.NJD00103.C09


title: m.NJD00103.C23


title: m.NJD00103.C15


title: m.NJD00103.C02


title: m.NJD00103.D02


title: m.NJD00103.C13


title: m.NJD00103.C04


title: m.NJD00103.D05


title: m.NJD00103.D07


title: m.NJD00103.C07


title: m.NJD00103.D04


title: m.NJD00103.C18


title: m.NJD00103.C20


title: m.NJD00103.B24


title: m.NJD00103.C16


title: m.NJD00103.B22


title: m.NJD00103.B21


title: m.NJD00103.D01


title: m.NJD00103.B20


title: m.NJD00103.B18


title: m.NJD00103.B19


title: m.NJD00103.B12


title: m.NJD00103.B15


title: m.NJD00103.B11


title: m.NJD00103.B13


title: m.NJD00103.B14


title: m.NJD00103.B17


title: m.NJD00103.B07


title: m.NJD00103.B02


title: m.NJD00103.A22


title: m.NJD00103.A19


title: m.NJD00103.A23


title: m.NJD00103.A16


title: m.NJD00103.A18


title: m.NJD00103.A15


title: m.NJD00103.A24


title: m.NJD00103.B09


title: m.NJD00103.B08


title: m.NJD00103.B16


title: m.NJD00103.B03


title: m.NJD00103.A20


title: m.NJD00103.A13


title: m.NJD00103.A14


title: m.NJD00103.B10


title: m.NJD00103.B04


title: m.NJD00103.A21


title: m.NJD00103.A17


title: m.NJD00103.A11


title: m.NJD00103.B06


title: m.NJD00103.A10


title: m.NJD00103.A05


title: m.NJD00103.A04


title: m.NJD00103.A09


title: m.NJD00103.A03


title: m.NJD00103.A01


title: m.NJD00103.A07


title: m.NJD00103.A08


title: m.NJD00104.P24


title: m.NJD00104.P18


title: m.NJD00103.A06


title: m.NJD00104.P23


title: m.NJD00104.P22


title: m.NJD00104.P06


title: m.NJD00104.P15


title: m.NJD00104.P14


title: m.NJD00104.P13


title: m.NJD00104.P09


title: m.NJD00104.P05


title: m.NJD00104.P07


title: m.NJD00104.P17


title: m.NJD00104.P08


title: m.NJD00104.P16


title: m.NJD00104.P20


title: m.NJD00104.P11


title: m.NJD00104.P02


title: m.NJD00104.P03


title: m.NJD00104.P04


title: m.NJD00104.P12


title: m.NJD00104.P19


title: m.NJD00104.P01


title: m.NJD00104.O24


title: m.NJD00104.O21


title: m.NJD00104.O22


title: m.NJD00104.O18


title: m.NJD00104.O17


title: m.NJD00104.O16


title: m.NJD00104.O15


title: m.NJD00104.O14


title: m.NJD00104.O06


title: m.NJD00104.O07


title: m.NJD00104.O10


title: m.NJD00104.O13


title: m.NJD00104.O08


title: m.NJD00104.O11


title: m.NJD00104.O12


title: m.NJD00104.N20


title: m.NJD00104.O02


title: m.NJD00104.N17


title: m.NJD00104.N18


title: m.NJD00104.N21


title: m.NJD00104.N23


title: m.NJD00104.N15


title: m.NJD00104.O20


title: m.NJD00104.O19


title: m.NJD00104.N13


title: m.NJD00104.N10


title: m.NJD00104.N11


title: m.NJD00104.O09


title: m.NJD00104.O01


title: m.NJD00104.N12


title: m.NJD00104.N24


title: m.NJD00104.N08


title: m.NJD00104.N09


title: m.NJD00104.N19


title: m.NJD00104.O04


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title: m.NJD00104.M22


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title: m.NJD00104.M13


title: m.NJD00104.M21


title: m.NJD00104.M20


title: m.NJD00104.N03


title: m.NJD00104.M17


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title: m.NJD00104.M23


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title: m.NJD00104.N01


title: m.NJD00104.M18


title: m.NJD00104.M06


title: m.NJD00104.N06


title: m.NJD00104.M05


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title: m.NJD00104.M03


title: m.NJD00104.L21


title: m.NJD00104.L24


title: m.NJD00104.M02


title: m.NJD00104.L23


title: m.NJD00104.L22


title: m.NJD00104.M01


title: m.NJD00104.L20


title: m.NJD00104.L19


title: m.NJD00104.M24


title: m.NJD00104.L18


title: m.NJD00104.M11


title: m.NJD00104.M10


title: m.NJD00104.L16


title: m.NJD00104.L17


title: m.NJD00104.L15


title: m.NJD00104.L14


title: m.NJD00104.L12


title: m.NJD00104.L11


title: m.NJD00104.L10


title: m.NJD00104.L05


title: m.NJD00104.L07


title: m.NJD00104.L13


title: m.NJD00104.L03


title: m.NJD00104.L04


title: m.NJD00104.L01


title: m.NJD00104.L08


title: m.NJD00104.L09


title: m.NJD00104.K24


title: m.NJD00104.K23


title: m.NJD00104.K22


title: m.NJD00104.K21


title: m.NJD00104.K19


title: m.NJD00104.K18


title: m.NJD00104.K10


title: m.NJD00104.K11


title: m.NJD00104.K09


title: m.NJD00104.K12


title: m.NJD00104.K15


title: m.NJD00104.K14


title: m.NJD00104.K07


title: m.NJD00104.K08


title: m.NJD00104.K05


title: m.NJD00104.K06


title: m.NJD00104.K02


title: m.NJD00104.K03


title: m.NJD00104.J23


title: m.NJD00104.J22


title: m.NJD00104.J19


title: m.NJD00104.J21


title: m.NJD00104.K20


title: m.NJD00104.K01


title: m.NJD00104.J17


title: m.NJD00104.J15


title: m.NJD00104.J13


title: m.NJD00104.J16


title: m.NJD00104.J12


title: m.NJD00104.J10


title: m.NJD00104.J24


title: m.NJD00104.J14


title: m.NJD00104.J08


title: m.NJD00104.J06


title: m.NJD00104.I23


title: m.NJD00104.I21


title: m.NJD00104.J01


title: m.NJD00104.I24


title: m.NJD00104.J04


title: m.NJD00104.J03


title: m.NJD00104.J07


title: m.NJD00104.I22


title: m.NJD00104.I17


title: m.NJD00104.I18


title: m.NJD00104.I19


title: m.NJD00104.I16


title: m.NJD00104.J11


title: m.NJD00104.I15


title: m.NJD00104.I11


title: m.NJD00104.I12


title: m.NJD00104.I10


title: m.NJD00104.J09


title: m.NJD00104.I09


title: m.NJD00104.I14


title: m.NJD00104.I05


title: m.NJD00104.I13


title: m.NJD00104.I04


title: m.NJD00104.I07


title: m.NJD00104.I06


title: m.NJD00104.I20


title: m.NJD00104.I02


title: m.NJD00104.H24


title: m.NJD00104.I01


title: m.NJD00104.H18


title: m.NJD00104.H23


title: m.NJD00104.H20


title: m.NJD00104.H22


title: m.NJD00104.H19


title: m.NJD00104.H21


title: m.NJD00104.H13


title: m.NJD00104.H16


title: m.NJD00104.I08


title: m.NJD00104.H12


title: m.NJD00104.H08


title: m.NJD00104.H05


title: m.NJD00104.H10


title: m.NJD00104.H07


title: m.NJD00104.H03


title: m.NJD00104.H04


title: m.NJD00104.H02


title: m.NJD00104.G22


title: m.NJD00104.G23


title: m.NJD00104.H01


title: m.NJD00104.G24


title: m.NJD00104.G20


title: m.NJD00104.G21


title: m.NJD00104.G19


title: m.NJD00104.G16


title: m.NJD00104.G15


title: m.NJD00104.G14


title: m.NJD00104.G12


title: m.NJD00104.H09


title: m.NJD00104.G11


title: m.NJD00104.G10


title: m.NJD00104.G08


title: m.NJD00104.G06


title: m.NJD00104.G02


title: m.NJD00104.F24


title: m.NJD00104.F22


title: m.NJD00104.F23


title: m.NJD00104.G03


title: m.NJD00104.F21


title: m.NJD00104.F20


title: m.NJD00104.F18


title: m.NJD00104.G04


title: m.NJD00104.F16


title: m.NJD00104.F19


title: m.NJD00104.F13


title: m.NJD00104.F14


title: m.NJD00104.F12


title: m.NJD00104.F09


title: m.NJD00104.F10


title: m.NJD00104.G17


title: m.NJD00104.F07


title: m.NJD00104.F06


title: m.NJD00104.G09


title: m.NJD00104.F03


title: m.NJD00104.F04


title: m.NJD00104.F02


title: m.NJD00104.F01


title: m.NJD00104.E24


title: m.NJD00104.E22


title: m.NJD00104.F17


title: m.NJD00104.E21


title: m.NJD00104.E20


title: m.NJD00104.F15


title: m.NJD00104.E16


title: m.NJD00104.E19


title: m.NJD00104.E10


title: m.NJD00104.E08


title: m.NJD00104.E03


title: m.NJD00104.E09


title: m.NJD00104.E13


title: m.NJD00104.E11


title: m.NJD00104.D23


title: m.NJD00104.E04


title: m.NJD00104.F05


title: m.NJD00104.E07


title: m.NJD00104.D22


title: m.NJD00104.E01


title: m.NJD00104.D20


title: m.NJD00104.D18


title: m.NJD00104.D17


title: m.NJD00104.D15


title: m.NJD00104.E18


title: m.NJD00104.E17


title: m.NJD00104.D11


title: m.NJD00104.D13


title: m.NJD00104.E12


title: m.NJD00104.D12


title: m.NJD00104.D10


title: m.NJD00104.D09


title: m.NJD00104.D08


title: m.NJD00104.D07


title: m.NJD00104.E06


title: m.NJD00104.E02


title: m.NJD00104.D03


title: m.NJD00104.D05


title: m.NJD00104.D04


title: m.NJD00104.C22


title: m.NJD00104.D06


title: m.NJD00104.D02


title: m.NJD00104.C24


title: m.NJD00104.C19


title: m.NJD00104.C20


title: m.NJD00104.C17


title: m.NJD00104.C21


title: m.NJD00104.C18


title: m.NJD00104.C15


title: m.NJD00104.C16


title: m.NJD00104.C14


title: m.NJD00104.C12


title: m.NJD00104.C11


title: m.NJD00104.C10


title: m.NJD00104.C08


title: m.NJD00104.C06


title: m.NJD00104.C07


title: m.NJD00104.C09


title: m.NJD00104.C05


title: m.NJD00104.C04


title: m.NJD00104.B24


title: m.NJD00104.C01


title: m.NJD00104.C23


title: m.NJD00104.B23


title: m.NJD00104.C03


title: m.NJD00104.C02


title: m.NJD00104.B17


title: m.NJD00104.B20


title: m.NJD00104.B22


title: m.NJD00104.B21


title: m.NJD00104.B18


title: m.NJD00104.B14


title: m.NJD00104.B16


title: m.NJD00104.B13


title: m.NJD00104.B15


title: m.NJD00104.B09


title: m.NJD00104.B12


title: m.NJD00104.B10


title: m.NJD00104.B07


title: m.NJD00104.B06


title: m.NJD00104.B08


title: m.NJD00104.B05


title: m.NJD00104.A19


title: m.NJD00104.B03


title: m.NJD00104.A18


title: m.NJD00104.A23


title: m.NJD00104.A16


title: m.NJD00104.A17


title: m.NJD00104.A13


title: m.NJD00104.A12


title: m.NJD00104.A11


title: m.NJD00104.A10


title: m.NJD00104.A09


title: m.NJD00104.A08


title: m.NJD00104.A06


title: m.NJD00104.A07


title: m.NJD00104.B11


title: m.NJD00104.A03


title: m.NJD00104.A05


title: m.NJD00104.A02


title: m.NJD00104.A01


title: s.NJA01901.P24

value: Singlet


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title: s.NJA01901.P22


title: s.NJA01901.P21


title: s.NJA01901.P18


title: s.NJA01901.P19


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title: s.NJA01901.P17


title: s.NJA01901.P13


title: s.NJA01901.P20


title: s.NJA01901.P08


title: s.NJA01901.P12


title: s.NJA01901.P14


title: s.NJA01901.P07


title: s.NJA01901.P09


title: s.NJA01901.P10


title: s.NJA01901.P11


title: s.NJA01901.P05


title: s.NJA01901.P04


title: s.NJA01901.P06


title: s.NJA01901.P03


title: s.NJA01901.P15


title: s.NJA01901.P01


title: s.NJA01901.P02


title: s.NJA01901.O19


title: s.NJA01901.O18


title: s.NJA01901.O20


title: s.NJA01901.O21


title: s.NJA01901.O23


title: s.NJA01901.O22


title: s.NJA01901.O14


title: s.NJA01901.O13


title: s.NJA01901.O16


title: s.NJA01901.O12


title: s.NJA01901.O24


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title: s.NJA01901.O09


title: s.NJA01901.O07


title: s.NJA01901.O08


title: s.NJA01901.O06


title: s.NJA01901.O02


title: s.NJA01901.N24


title: s.NJA01901.O04


title: s.NJA01901.N23


title: s.NJA01901.N22


title: s.NJA01901.O17


title: s.NJA01901.N21


title: s.NJA01901.N20


title: s.NJA01901.O15


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title: s.NJA01901.O11


title: s.NJA01901.N15


title: s.NJA01901.N16


title: s.NJA01901.N17


title: s.NJA01901.N13


title: s.NJA01901.O03


title: s.NJA01901.N12


title: s.NJA01901.N11


title: s.NJA01901.O01


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title: s.NJA01901.N06


title: s.NJA01901.N04


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title: s.NJA01901.M24


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title: s.NJA01901.M22


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title: s.NJA01901.M20


title: s.NJA01901.M21


title: s.NJA01901.N14


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title: s.NJA01901.N09


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title: s.NJA01901.M13


title: s.NJA01901.N07


title: s.NJA01901.N08


title: s.NJA01901.M12


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title: s.NJA01901.M11


title: s.NJA01901.M08


title: s.NJA01901.M07


title: s.NJA01901.M06


title: s.NJA01901.N01


title: s.NJA01901.M05


title: s.NJA01901.M04


title: s.NJA01901.M01


title: s.NJA01901.M02


title: s.NJA01901.M03


title: s.NJA01901.L23


title: s.NJA01901.L24


title: s.NJA01901.L22


title: s.NJA01901.L20


title: s.NJA01901.L21


title: s.NJA01901.L19


title: s.NJA01901.M15


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title: s.NJA01901.L18


title: s.NJA01901.L16


title: s.NJA01901.L14


title: s.NJA01901.L15


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title: s.NJA01901.L12


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title: s.NJA01901.L11


title: s.NJA01901.L08


title: s.NJA01901.L07


title: s.NJA01901.L04


title: s.NJA01901.L10


title: s.NJA01901.L06


title: s.NJA01901.L02


title: s.NJA01901.L01


title: s.NJA01901.K24


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title: s.NJA01901.K22


title: s.NJA01901.K23


title: s.NJA01901.K18


title: s.NJA01901.K19


title: s.NJA01901.K20


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title: s.NJA01901.K15


title: s.NJA01901.K13


title: s.NJA01901.K14


title: s.NJA01901.K16


title: s.NJA01901.K21


title: s.NJA01901.K10


title: s.NJA01901.K12


title: s.NJA01901.K09


title: s.NJA01901.K08


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title: s.NJA01901.K11


title: s.NJA01901.K06


title: s.NJA01901.K05


title: s.NJA01901.K02


title: s.NJA01901.K03


title: s.NJA01901.K07


title: s.NJA01901.K01


title: s.NJA01901.K04


title: s.NJA01901.J21


title: s.NJA01901.J24


title: s.NJA01901.J23


title: s.NJA01901.J20


title: s.NJA01901.J18


title: s.NJA01901.J16


title: s.NJA01901.J13


title: s.NJA01901.J14


title: s.NJA01901.J12


title: s.NJA01901.J11


title: s.NJA01901.J10


title: s.NJA01901.J09


title: s.NJA01901.J06


title: s.NJA01901.J05


title: s.NJA01901.J08


title: s.NJA01901.J03


title: s.NJA01901.J07


title: s.NJA01901.J02


title: s.NJA01901.J01


title: s.NJA01901.J04


title: s.NJA01901.J22


title: s.NJA01901.J19


title: s.NJA01901.I23


title: s.NJA01901.I22


title: s.NJA01901.I21


title: s.NJA01901.I18


title: s.NJA01901.I17


title: s.NJA01901.I24


title: s.NJA01901.I19


title: s.NJA01901.I20


title: s.NJA01901.I14


title: s.NJA01901.I13


title: s.NJA01901.I15


title: s.NJA01901.J17


title: s.NJA01901.I09


title: s.NJA01901.I11


title: s.NJA01901.I10


title: s.NJA01901.I08


title: s.NJA01901.I06


title: s.NJA01901.I07


title: s.NJA01901.I03


title: s.NJA01901.I04


title: s.NJA01901.I01


title: s.NJA01901.I02


title: s.NJA01901.I05


title: s.NJA01901.H23


title: s.NJA01901.H24


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title: s.NJA01901.H21


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title: s.NJA01901.I12


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title: s.NJA01901.H16


title: s.NJA01901.H09


title: s.NJA01901.H11


title: s.NJA01901.H14


title: s.NJA01901.H15


title: s.NJA01901.H13


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title: s.NJA01901.H06


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title: s.NJA01901.G24


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title: s.NJA01901.G23


title: s.NJA01901.G21


title: s.NJA01901.G20


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title: s.NJA01901.G13


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title: s.NJA01901.G12


title: s.NJA01901.G08


title: s.NJA01901.G10


title: s.NJA01901.H03


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title: s.NJA01901.G05


title: s.NJA01901.G06


title: s.NJA01901.G04


title: s.NJA01901.G03


title: s.NJA01901.H02


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title: s.NJA01901.F15


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title: s.NJA01901.F24


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title: s.NJA01901.E24


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title: s.NJA01901.E20


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title: s.NJA01901.E12


title: s.NJA01901.E11


title: s.NJA01901.E10


title: s.NJA01901.E08


title: s.NJA01901.E09


title: s.NJA01901.E07


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title: s.NJA01901.E04


title: s.NJA01901.E06


title: s.NJA01901.E02


title: s.NJA01901.E03


title: s.NJA01901.D24


title: s.NJA01901.E01


title: s.NJA01901.D23


title: s.NJA01901.D22


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title: s.NJA01901.D02


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title: s.NJA01901.B22


title: s.NJA01901.C09


title: s.NJA01901.B24


title: s.NJA01901.C06


title: s.NJA01901.C08


title: s.NJA01901.B19


title: s.NJA01901.B17


title: s.NJA01901.B20


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title: s.NJA01901.B06


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title: s.NJA02001.M13


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title: s.NJA02001.M11


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title: s.NJA02001.M07


title: m.NJA02001.L24


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title: m.NJA02001.L21


title: m.NJA02001.L23


title: s.NJA02001.M02


title: m.NJA02001.L22


title: m.NJA02001.L20


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title: s.NJA02001.L16


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title: s.NJA02001.L12


title: s.NJA02001.L11


title: s.NJA02001.L09


title: s.NJA02001.L07


title: s.NJA02001.L08


title: s.NJA02001.L06


title: s.NJA02001.L05


title: s.NJA02001.L04


title: s.NJA02001.L03


title: s.NJA02001.L01


title: s.NJA02001.L02


title: m.NJA02001.K23


title: m.NJA02001.K24


title: m.NJA02001.K22


title: m.NJA02001.K21


title: m.NJA02001.K19


title: s.NJA02001.K17


title: s.NJA02001.K16


title: s.NJA02001.K14


title: s.NJA02001.K13


title: s.NJA02001.K11


title: s.NJA02001.K10


title: m.NJA02001.K20


title: s.NJA02001.K09


title: s.NJA02001.K07


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title: s.NJA02001.B12


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title: s.NJA02001.A02


title: s.NJA02001.A04


title: s.NJA02001.A01


title: m.NJA02001.A21


title: Tie1-PC-rep4

city: Heidelberg

first: Jingjing

last: Shi

state: Baden-Wuerttemberg

address: Im Neuenheimer Feld 280

department: Vascular Oncology and Metastasis

country: Germany

institute: German Cancer Research Center

data_processing:
Illumina output files were demultiplexed with bcl2fastq2 Conversion Software v2.20 (Illumina).
Adapter Sequences were removed by Cutadapt and the trimmed output were aligned to the transcriptome index of the GRCm38.92 (Ensembl) using RNA STAR (Galaxy) and gene expression were measured by featureCounts
Transcripts per Million (TPM) was calculated for gene expression
Genome_build: mm10
Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every sample

source_name: liver endothelial cell

extract_protocol:
RNA extracton from PicoPure RNA Isolation kit (Thermo Fisher Scientific) with on-column DNase I (Qiagen) genomic DNA removal following manufacturer's instructions.
Library was prepared TruSeq Stranded mRNA library Prep kit (Illumina, Cat 20020594) following manufacturer's instructions.

treatment_protocol: Tie1  treatment

tag: treatment

value: liver

tag: cell type

value: liver endothelial cell

tag: subpopulation (zone)

value: peri-central


title: Tie1-CV-rep4

value: central vein


title: Tie1-PP-rep4

value: peri-portal


title: Tie1-PN-rep4

value: portal node


title: m.NJA02001.A23


title: WT-CV-rep4

value: control


title: WT-PC-rep4


title: Tie1-PN-rep3


title: s.NJA02001.A11


title: WT-PP-rep4


title: Tie1-CV-rep3


title: s.NJA02001.A09


title: Tie1-PC-rep3


title: Tie1-PP-rep3


title: WT-CV-rep3


title: WT-PN-rep3


title: WT-PP-rep3


title: Tie1-CV-rep2


title: s.NJA02001.A05


title: Tie1-PC-rep2


title: WT-PC-rep3


title: s.NJA02001.A03


title: Tie1-PP-rep2


title: Tie1-PN-rep2


title: WT-PC-rep2


title: WT-CV-rep2


title: WT-PN-rep2


title: Tie1-CV-rep1


title: Tie1-PN-rep1


title: Tie1-PP-rep1


title: WT-CV-rep1


title: WT-PP-rep2


title: WT-PC-rep1


title: WT-PP-rep1


title: WT-PN-rep4


title: WT-PN-rep1


title: Tie1-PC-rep1


title: m.NJA01303.P24


title: s.NJA01303.P18


title: s.NJA01303.P16


title: s.NJA01303.P15


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title: s.NJA01303.O04


title: s.NJA01303.O05


title: s.NJA01303.O03


title: m.NJA01303.N19


title: s.NJA01303.O02


title: m.NJA01303.N22


title: s.NJA01303.N17


title: m.NJA01303.N24


title: m.NJA01303.N21


title: m.NJA01303.N20


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title: s.NJA01303.C04


title: s.NJA01303.C03


title: s.NJA01303.C16


title: s.NJA01303.C02


title: s.NJA01303.C01


title: m.NJA01303.B24


title: s.NJA01303.B15


title: s.NJA01303.B18


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title: m.NJA01303.A24


title: m.NJA01303.A22


title: m.NJA01303.A20


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title: Sg3

city: Guangzhou

first: Tingjun

last: Liu

address: 56 Lingyuanxi Road

department: Hospital of Stomatology, Guanghua School of Stomatology

country: China

institute: Sun Yat-sen University

data_processing:
The raw paired end reads were trimmed and quality controlled by Trimmomatic with parameters (SLIDINGWINDOW:4:15 MINLEN:75) (version 0.36 http://www.usadellab.org/cms/uploads/supplementary/Trimmomatic). Then clean reads were separately aligned to reference genome with orientation mode using Rockhopper (http://cs.wellesley.edu/~btjaden/Rockhopper/) software. Rockhopper was a comprehensive and user-friendly system for computational analysis of bacterial RNA-seq data. As input, Rockhopper takes RNA sequencing reads generated by high-throughput sequencing technology. This software was used to calculate gene expression levels with default parameters.
To identify DEGs (differential expression genes) between the two different samples, the expression level for each transcript was calculated using the fragments per kilobase of read per million mapped reads (RPKM) method. edgeR (https://bioconductor.org/packages/release/bioc/html/edgeR.html) was used for differential expression analysis. The DEGs between two samples were selected using the following criteria: i) the logarithmic of fold change was greater than 2 and the false discovery rate (FDR) should be less than 0.05.
To understand the functions of the differential expressed genes, GO functional enrichment and KEGG pathway analysis were carried out by Goatools (https://github.com/tanghaibao/Goatools) and KOBAS (http://kobas.cbi.pku.edu.cn/home.do) respectively. DEGs were significantly enriched in GO terms and metabolic pathways when their Bonferroni-corrected P-value was less than 0.05.
Genome_build: Beta_vulgaris.GCF_000511025.2_RefBeet-1.2.2
Supplementary_files_format_and_content: tab-delimited text files include counts values for each Sample: values for S. gordonii DL1 in mono-species group (Sg) as well as in coaggregation group (CAB) were reported in the processed file "CAB(Sg)_vs_Sg.genes.read.txt"; values for F. nucleatum subsp. polymorphum (ATCC10953) in mono-species group (Fnp) as well as in the coaggregation group (CAB) were reported in the processed file "CAB(Fnp)_vs_Fnp.genes.read.txt".

organism: Streptococcus gordonii str. Challis substr. CH1

source_name: single species

growth_protocol: S. gordonii DL1 was grown in Brain Heart Infusion (BHI) broth (Difco, USA). F. nucleatum subsp. polymorphum (ATCC10953) was grown in BHI broth supplemented with 0.5% yeast extract (Difco, USA), 1 μg/mL vitamin K (Sigma-Aldrich, USA), and 5 μg/ml hemin (Sigma-Aldrich, USA). Both bacterial strains were grown under anaerobic conditions (85% N2, 10% H2, 5% CO2) at 37 °C.

extract_protocol:
Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (±50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq™ 6000 at the (lc-bio, China) following the vendor's recommended protocol.
RNA libraries were prepared for sequencing using standard Illumina protocols

treatment_protocol: Intergeneric coaggregation was performed in coaggregation buffer (CAB) (150 mM NaCl, 1 mM Tris pH 8, 0.1 mM CaCl2, 0.1 mM MgCl2) as previously described [37]. The optical density (OD600) of F. nucleatum subsp. polymorphum after 24-hour culture reached 0.85 (~109 CFU), and the OD600 of S. gordonii DL1 after 12-hour culture was assessed to be 0.7 (~109 CFU). Bacterial cells of each species were harvested, washed twice, and resuspended in CAB to a final concentration of ~2×109 cells/ml (OD600~1.0). Equal numbers of cells from each species were pooled together in a new sterile reaction tube. The reaction tube was vortexed for 20 seconds to maximize bacterial physical contact and incubated anaerobically at 37℃ for 30 min for coaggregation.

tag: growth protocol

value: monoculture

tag: gram status

value: Gram-positive species


title: Sg2


title: m.NJA01501.O20


title: s.NJA01501.P11


title: s.NJA01501.O18


title: s.NJA01501.O17


title: C_HC_632_M_367

city: Nouzilly

first: Vincent

last: Coustham

address: INRAE Centre Val de Loire

country: France

institute: INRAE

data_processing:
Image analysis and base calling were performed using RTA 2.7.3 and bcl2fastq 2.17.1.14.
Sequenced reads were mapped to Coturnix japonica 2.0 genome using STAR with default arguments
Reads were counted using FeatureCounts with arguments -T 6 -s 2 -t exon -g gene and the annotation file GCF_001577835.1_Coturnix_japonica_2.0_genomic.gff
Biostatistical analysis was performed using the Bioconductor edgeR package (version 3.24.3); p-values were adjusted by controlling the false positive rate below 0.05 with a Benjamini-Hochberg correction
Genome_build: Coturnix japonica 2.0, GCA_001577835.1
Supplementary_files_format_and_content: tab-delimited text files including normalized counts and adjusted p-values
Supplementary_files_format_and_content: processed counts for all samples: S19041.processed.counts.txt
Supplementary_files_format_and_content: edgeR analysis for FCHC vs FCRT comparison: FCHCvsFCRT.complete.txt
Supplementary_files_format_and_content: edgeR analysis for FCHC vs FTMHC comparison: FCHCvsFTMHC.complete.txt
Supplementary_files_format_and_content: edgeR analysis for FCRT vs FTMRT comparison: FCRTvsFTMRT.complete.txt
Supplementary_files_format_and_content: edgeR analysis for FTMHC vs FTMRT comparison: FTMHCvsFTMRT.complete.txt
Supplementary_files_format_and_content: edgeR analysis for MCHC vs MCRT comparison: MCHCvsMCRT.complete.txt
Supplementary_files_format_and_content: edgeR analysis for MCHC vs MTMHC comparison: MCHCvsMTMHC.complete.txt
Supplementary_files_format_and_content: edgeR analysis for MCRT vs MTMRT comparison: MCRTvsMTMRT.complete.txt
Supplementary_files_format_and_content: edgeR analysis for MTMHC vs MTMRT comparison: MTMHCvsMTMRT.complete.txt

molecule: polyA RNA

organism: Coturnix japonica

source_name: Total RNA samples produced with Qiagen Allprep mini kit.

extract_protocol:
RNA extraction was performed using All Prep RNA/DNA Mini kit (Qiagen), according to the manufacturer’s instruction. RNA concentration was obtained on a Nanodrop ND-1000 UV-Vis Spectrophotometer. RNA integrity was verified by on an Agilent 2100 bioanalyzer with a RNA Nano kit (Agilent), according to the manufacturer’s instruction. All sample RIN was above 8.5.
RNA-Seq libraries were generated from 500ng of total RNA using TruSeq Stranded mRNA Library Prep Kit and TruSeq RNA Single Indexes kits A and B (Illumina, San Diego, CA), according to manufacturer's instructions. Briefly, following purification with poly-T oligo attached magnetic beads, the mRNA was fragmented using divalent cations at 94oC for 2 minutes. The cleaved RNA fragments were copied into first strand cDNA using reverse transcriptase and random primers. Strand specificity was achieved by replacing dTTP with dUTP during second strand cDNA synthesis using DNA Polymerase I and RNase H. Following addition of a single 'A' base and subsequent ligation of the adapter on double stranded cDNA fragments, the products were purified and enriched with PCR (30 sec at 98oC; [10 sec at 98oC, 30 sec at 60oC, 30 sec at 72oC] x 12 cycles; 5 min at 72oC) to create the cDNA library. Surplus PCR primers were further removed by purification using AMPure XP beads (Beckman-Coulter, Villepinte, France) and the final cDNA libraries were checked for quality and quantified using capillary electrophoresis.

treatment_protocol: Eggs of Cons DD Japanese quail line were dispatched between two embryonic treatments: Control incubation (C) or Thermo-Manipulated incubation (TM). C eggs were maintained at 37.8°C and 56% relative humidity (RH) during the whole incubation period. TM eggs were incubated at 39.5°C and 65% RH for 12 h/d from I0 to I13 and incubated at 37.8°C 56 % RH the rest of the time. At D35, half of the quails (same proportion of C and TM animals) was kept at room temperature (RT) while the other half was submitted to a heat challenge (HC - 36°C for 7 hours).

value: hypothalamus

value: Cons DD

tag: Sex

value: Male

tag: incubation condition

value: Control

tag: challenge condition (at age day 35)

value: Heat challenge

tag: age

value: post natal day 35


title: m.NJA01501.O23

value: Multiplet


title: C_HC_642_M_362


title: C_HC_754_M_267


title: s.NJA01501.O16

value: Singlet


title: s.NJA01501.O15


title: s.NJA01501.P15


title: C_HC_871_M_252


title: C_HC_611_M_262


title: C_HC_872_F_347

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title: s.NJA01501.O13


title: s.NJA01501.O14


title: C_HC_785_F_332


title: s.NJA01501.O12


title: C_HC_869_F_247


title: s.NJA01501.O09


title: Sg1


title: C_HC_895_M_372


title: s.NJA01501.O10


title: C_HC_858_F_327


title: C_HC_896_F_227


title: C_RT_668_M_172

value: Room temperature


title: s.NJA01501.O08


title: C_RT_894_M_167


title: s.NJA01501.O07


title: C_RT_879_M_72


title: C_RT_877_M_162


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title: s.NJA01501.O04


title: s.NJA01501.O03


title: s.NJA01501.O02


title: C_RT_893_M_67


title: C_RT_691_F_147


title: C_RT_741_M_62


title: m.NJA01501.N24


title: s.NJA01501.O11


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title: m.NJA01501.N23


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title: s.NJA01501.N17


title: C _RT_655_F_27


title: s.NJA01501.N16


title: TM_HC_1079_M_392


title: TM_HC_1050_M_297


title: TM_HC_1130_M_292


title: s.NJA01501.N12


title: s.NJA01501.N11


title: s.NJA01501.N07


title: TM_HC_1245_F_302


title: s.NJA01501.N10


title: s.NJA01501.N09


title: TM_HC_1039_F_222


title: TM_HC_1269_F_312


title: TM_HC_1155_F_217


title: s.NJA01501.N05


title: m.NJA01501.N20