title: mESC_DNA_cell4809


title: mESC_DNA_cell4819

status: Public on May 14 2021

type: SRA

city: Beijing

first: Haiqing

last: Xiong

state: Beijing

address: 5 Yiheyuan Road,  Haidian District

country: China

institute: Peking University

library_source: genomic

data_processing:
Basecalls performed using CASAVA version 1.8.2.
For ChIP part, Reads were de-multiplexed to generate single cell profiles by custom scripts.
Reads were aligned to mouse genome mm10 by bowtie2 (v 2.3.1) (ChIP part) and Hisat2 (version 2.0.4) (RNA part).
ChIP part:  Reads whose MAPQ >= 30 were determined as uniquely mapping reads. Duplication reads were removed by Picard. RNA part: reads were counted by HTSeq.
Genome_build: mm10
Supplementary_files_format_and_content: The processed files were aggregate bigwig files.

molecule: genomic DNA

organism: Mus musculus

source_name: cell line

growth_protocol: mESCs were cultured at 37 °C with 5% CO2 and was maintained in high glucose DMEM

extract_protocol:
folloing CoTECH protocol
After PCR enrichment using Q5 polymerase, the 300-1,000 bp fragments were isolated using the 1.5% (w/v) agarose gel and purified once with 0.8 x AMpure XP beads

treatment_protocol: The mESCs were dissociated by 0.1% Trypsin digestion, and aliquot of cell numbers were directly used for CoTECH

tag: cell type

value: cultured mouse embryonic stem cells

tag: strain

tag: chip antibody


title: mESC_DNA_cell4815


title: mESC_DNA_cell4805


title: mESC_DNA_cell4816


title: mESC_DNA_cell4807


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