title: BFF116 cells replicate 3, nuclear donor cells

status: Public on May 31 2021

type: SRA

city: Munich

first: Alexander

last: Graf

address: Feodor-Lynen-Str. 25

department: Gene Center Munich

country: Germany

institute: Ludwig-Maximilians-Universität München

library_source: transcriptomic

data_processing:
Reads were converted to Fastq using the bcl2fastq (v2.18.0.12) converter from Illumina.
Illumina adapters where clipped from each read using a custom script.
Poly-A tails were removed from the 3’-end of each read with a seed length of 10 nt. Reads with length <20nt after clipping were filtered out.
Reads were filtered from the 3′- and 5′-end with a quality cutoff of 20, and reads shorter than 30nt were discarded.
Reads were mapped against the reference genome (BosTau7) using Tophat2 (v.2.0.10) with default settings and the gene annotation obtained from Illumina’s iGenomes Project (UCSC: bosTau7).
Transcript abundance was counted using htseq-count (v.0.6.1) with no strand specificity (-s no), union mode (-m union) and GFF attribute ‘gene’ (-i gene). The used gene annotation was obtained from Illumina’s iGenome Project (UCSC: bosTau7).
Genome_build: BosTau7
Supplementary_files_format_and_content: Tab-delimited text file containing the read counts per gene

molecule: total RNA

organism: Bos taurus

source_name: bovine fetal fibroblast nuclear donor cells

growth_protocol: Bovine fetal fibroblast nuclear donor cells (BFF116) were cultured as published (Wuensche A, et al., 2007, Bauersachs S, et al., 2009).

extract_protocol:
Cells (7 × 104)  for RNA extraction were prepared as donor cells for SCNT. Briefly, SCNT procedures were carried out as described previously (Bauersachs S, et al., 2009): 48 h before SCNT,  the cell cycle of donor cells was synchronized by serum starvation. Cells were harvested, snap-frozen and stored at ‑80 °C until analysis.
Frozen cells were thawed and lysed in 10 µL of lysis buffer (Prelude kit, NuGEN). The Ovation RNAseq v2 kit (NuGEN) was used for cDNA generation and single primer isothermal amplification (SPIA). The amplified cDNA was bead-purified (AmpureXP, Beckman-Coulter) and fragmented by sonication (Bioruptor, Diagenode; 25 cycles 30 seconds on/30 seconds off), and Illumina-compatible sequencing libraries were constructed from 500 ng of fragmented cDNA by ligation of adapters with sample-specific barcodes. The libraries were amplified (KAPA hifi polymerase, ten cycles, 95 °C 80 seconds, 55 °C 30 seconds, 72 °C 60 seconds) and quantified on a Bioanalyzer 2100 (Agilent) before being pooled at 10 nM concentration for multiplexed sequencing. Three biological replicates were sequenced on an Illumina HiSeq1500 (single-read, mean coverage of 20 × 106 reads, 100-base read length).

tag: developmental stage

value: nuclear donor cells


title: BFF116 cells replicate 1, nuclear donor cells


title: ΔF6 rep3

city: Moscow

first: Artem

last: Grigorov

address: Ulitsa Miklukho-Maklaya, 16/10

department: Genomics and Postgenomic Technologies

country: Russia

institute: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry

data_processing:
Sequenced reads were mapped to M.tuberculosis H37Rv genome using bowtie2 v2.3.4.3 with parameters -p 4 --local --dovetail
Fragments counting was performed using FeatureCounts (Annotation of the genome was retrieved from Mycobrowser). For every annotated gene, the number of fragments overlapping the gene at least by one nucleotide was calculated. Only unambiguously mapped, non-chimeric fragments were used. The files "Counts_XXX.txt" represent these counts (File "Counts_all.csv" contains values for all samples).
Search for differentially expressed genes was done with DESeq2 package (using only CDS transcripts). The results are represented in file "DifferentialExpression.csv"
Genome_build: NC_008596.1
Supplementary_files_format_and_content: Counts - the numbers of overlapping reads for every annotated gene in each sample; DifferentialExpression.csv - the results of search for differentially expressed genes using DESeq2 package.

organism: Mycolicibacterium smegmatis

source_name: bacterial culture

growth_protocol: M.smegmatis MC2 155 WT and ΔF6 strains were grown up to the logarithmic phase (OD ~ 1.0) in Middlebrook 7H9 media supplement with 0.05% Tween 80.

extract_protocol:
Bacterial cultures were grown up to the logarithmic phase (OD ~ 1.0), centrifuged (4°C, 3000 rpm) and total RNA was isolated by phenol-chloroform extraction after cell disruption by Bead Beater with 0.1 mm zirconia beads (BioSpec Products, USA) as previously described (Rustad et al., 2009). After isolation, RNA was treated with Turbo DNase (Life Technologies, USA) to remove traces of genomic DNA, and purified with the RNeasy mini kit (Qiagen, Netherlands). Amounts and purity of RNA were determined spectrophotometrically; integrity of RNA was assessed in 1% agarose gel.
RNA samples were depleted of rRNA using Ribo-Zero rRNA Removal Kit (Bacteria). Sequencing libraries  were generated using the resulting ribosomal transcript-depleted RNA and NEBNext Ultra II Directional  RNA Library Prep Kit (NEB, USA) according to the manufacturers' protocol. Sequencing was performed in  triplicates using the Illumina HiSeq2500 as the pair-ended 150 nt-long reads.

tag: strain

tag: phase

value: logarithmic

tag: genotype


title: ΔF6 rep2


title: WT rep1

value: WT


title: ΔF6 rep1


title: minus Cd mRNA

city: Beijing

first: Hong

middle: Yu

last: Zhang

address: Qinglongqiao Street

department: Research Institute of Forestry

country: China

institute: Chinese Academy of Forestry

description:
minus Cd
mRNA

data_processing:
Raw reads were obtained using Illumina’s Pipeline (Version 1.5) following sequencing image analysis and base-calling
Potentially cleaved transcripts were identified by CleaveLand (version 3.0).
The high-quality reads were mapped to the genome sequence of P. trichocarpa using Tophat package (version 2.1.1)
The FPKMs were calculated using a protocol from Pertea et al. Nature Biotechnology, 2015
Genome_build: version 3.0
Supplementary_files_format_and_content: Tab-defined each excel files include FPKMs for each samples

organism: Populus deltoides

source_name: Leaf

growth_protocol: Stem cuttings of Populus deltoides were rooted and planted in plastic pots and irrigated with 50 mL modified Hoagland nutritional solution in the evening of every other day. After 8 weeks, 72 plants with similar height and growth performance were selected and cultivated in hydroponics using modified Hoagland nutritional solution which was refreshed every 2 days.

extract_protocol:
Total RNA was extracted in the leaves of poplars using Trizol reagent (Invitrogen, CA, USA) following the manufacturer’s procedure with minor modifications. The quantity and purity (RIN number >7.0) of the extracted RNA were analyzed by Bioanalyzer 2100 (Agilent, Santa Clara, CA, USA) and RNA 6000 Nano LabChip Kit (Agilent, CA, USA). Afterwards, the extracted RNA samples were treated with total RNA purification kit (TRK1001, LC Science, Houston, TX) to eliminate genomic DNA contamination.
For mRNA sequencing, the purified total RNA from each sample was reverse-transcribed to create the cDNA library according to the protocol of the RNA-sequencing sample preparation kit (Illumina, San Diego, USA). For small RNA sequencing, the extracted RNA was used to construct a small RNA library using TruSeq Small RNA Prep Kits (RS-200, Illumina, CA, USA). For degradome sequencing, equal amounts of the 9 RNA samples from poplar leaves treated with either 0 or 50 µM CdCl2 were mixed to form a pooled sample. About 150 ng of RNA with poly(A) was annealed and captured with biotinylated random primers. Then, the RNAs containing 5’-monophosphates were ligated to 5’ adaptor and transcribed to cDNAs
Total mRNA sequencing was performed on an Illumina Hiseq2000/2500 sequencer (Illumina, CA, USA).The single-end sequencing was performed on an Illumina Hiseq2500 sequencer (Illumina, CA, USA) at the LC Science (Hangzhou, China) following the vendor’s recommended protocol for  small RNA sequenceing and degradome sequencing.

treatment_protocol: After treated with 0 (low, L), 100 (middle, M), or 1500 (high, H) µM SO42- for 12 days, the plants were exposed to either 0 (-Cd) or 50 (+Cd) μM CdCl2 together with the S treatments for 21 additional days.

value: monilifera (Aiton) Eckenw

tag: culture

value: sand culture + hydroponics


title: WT rep3


title: plus CdH2 small RNA

description:
plus CdH2
small RNA


title: plus CdH3 small RNA

description:
plus CdH3
small RNA


title: WT rep2


title: Swimming exercise training_S15

type: RNA

city: Shanghai

first: Lijun

last: Wang

address: 333 Nan Chen Road

institute: Shanghai University

description: Heart from mice that after swimming exercise training for 3 weeks, which is exercise-induced cardiac physiological hypertrophy model

hyb_protocol: The purified cRNA were hybridized for 17 hr at 65°C on Agilent Mouse lncRNA array（4*180K，Design ID：049801）.  After hybridization, microarrays were washed 1 minute at room temperature with Gene Expression Wash Buffer 1 (Agilent) and 1 minute with 37°C Gene Expression Wash Buffer 2 (Agilent).

scan_protocol: After washing, the arrays were scanned with the Agilent Scanner G2505C (Agilent Technologies).

data_processing:
Feature Extraction software (version10.7.1.1, Agilent Technologies)was used to analyze array images to get raw data. Genespring were employed to finish the basic analysis with the raw data. To begin with, the raw data was normalized with the quantile algorithm. The probes that at least 100% of the values in any 1 out of all conditions have flags in P were chosen for further data analysis.
Differentially expressed genes were then identified through fold change as well as P value calculated with t-test.

organism: Mus musculus

label_protocol: Total RNA were reverse transcribed into cDNA, labeled with Cyanine-3-CTP, and then synthesized to cRNA.

growth_protocol: All mice maintained in specific pathogen-free (SPF) laboratory animal facility of Nanjing Medical University (Nanjing, China). All procedures with animals followed guidelines on the use and care of laboratory animals for biomedical research published by National Institutes of Health, and approved by the committee on the Ethics of Animal Experiments of Nanjing Medical University.

extract_protocol: Total RNA were isolated using mirVanaTM RNA Isolation Kit according to the manufacturer's instructions.

treatment_protocol: To induce physiological cardiac hypertrophy, a 3-week swimming protocol was performed in mice. Briefly speaking, the exercise training began with twice-a-day for 10 min swimming for the first day, and then continued with an increase of 10 min per day until twice-a-day 90 min swimming per day was reached. After 3 weeks of swimming, the mice were sacrificed and cardiac tissues were collected.

tag: Sex

value: male

tag: age

value: 11 week

tag: treatment

value: 3-week swimming protocol

tag: tissue

value: heart


title: plus CdM2 small RNA

description:
plus CdM2
small RNA


title: plus Cd mRNA

description:
plus Cd
mRNA


title: Sedentary(Control)_C12

description: Heart from mice that without swimming exercise training (sedentary for 3 weeks),which is control

value: no exercise; sedentary


title: Sedentary(Control)_C14

source_name: Heart from mice that without swimming exercise training (sedentary for 3 weeks)


title: plus CdM1 small RNA

description:
plus CdM1
small RNA


title: Sedentary(Control)_C11


title: Swimming exercise training_S13

source_name: Heart from mice that after swimming exercise training for 3 weeks


title: Swimming exercise training_S14


title: Swimming exercise training_S11


title: plus CdL3 small RNA

description:
plus CdL3
small RNA


title: minus CdM3 small RNA

description:
minus CdM3
small RNA


title: minus CdH3 small RNA

description:
minus CdH3
small RNA


title: minus CdH2 small RNA

description:
minus CdH2
small RNA


title: minus CdL3 small RNA

description:
minus CdL3
small RNA


title: BFF116 cells replicate 2, nuclear donor cells


title: plus CdM3 small RNA

description:
plus CdM3
small RNA


title: plus CdH1 small RNA

description:
plus CdH1
small RNA


title: plus CdL1 small RNA

description:
plus CdL1
small RNA


title: Swimming exercise training_S12


title: minus CdH1 small RNA

description:
minus CdH1
small RNA


title: minus CdM1 small RNA

description:
minus CdM1
small RNA


title: minus CdM2 small RNA

description:
minus CdM2
small RNA


title: plus CdL2 small RNA

description:
plus CdL2
small RNA


title: minus CdL2 small RNA

description:
minus CdL2
small RNA


title: minus CdL1 small RNA

description:
minus CdL1
small RNA


title: R-1101_UWB1.289+BRCA1_40uM_CT_M1

city: Dresden

first: Konrad

last: Grützmann

address: Schubertstraße 15

department: Core Unit for Molecular Tumor Diagnostics

institute: National Center for Tumor Diseases partner site Dresden

data_processing:
Raw reads were converted from bcl to fastq format using bcl2fastq (version v2.17.1.14) allowing for 1 barcode mismatch
Reads were trimmed for quality, sequence adapters and cropped to 75nt using trimmomatic 0.36 with the following parameters: ILLUMINACLIP:TruSeq3-PE.fa:2:30:10:2:true LEADING:15 TRAILING:15 SLIDINGWINDOW:4:15 MINLEN:36 CROP:75
Reads were aligned against the genome STAR 2.5.1 in a 2-pass mapping mode: first, an index was created using the genome sequence and gene annotation (here, Gencode GRCh38.p7 comprehensive gene annotation), against which all reads were aligned. Second, all detected splice junctions of all samples were merged and used as guide for the second mapping step. The following parameters were used in both steps: --readFilesCommand zcat --alignIntronMax 500000 --alignMatesGapMax 500000 --outSAMtype BAM SortedByCoordinate --outSAMprimaryFlag OneBestScore --outFilterMultimapNmax 100 --outFilterMismatchNoverLmax 0.05 --chimSegmentMin 15 --chimScoreMin 1 --chimScoreJunctionNonGTAG 0 --chimJunctionOverhangMin 15 --chimSegmentReadGapMax 3 --alignSJstitchMismatchNmax 5 -1 5 5. For the second step, additional parameters are: --limitSjdbInsertNsj 10000000 --limitBAMsortRAM 20000000000 --limitGenomeGenerateRAM 20000000000 --sjdbFileChrStartEnd allSJ.out.tab, where allSJ. out.tab denotes the collected splice junctions.
Read counts of all annotated genes were extracted from the alignments using the featureCounts function of the Rsubread package (v1.20.6) with the following parameters: GTF.featureType = “exon”, GTF.attrType=“gene_id”, useMetaFeatures = T, isPairedEnd=T, requireBothEndsMapped=F, allowMultiOverlap=T, countMultiMappingReads=F, fraction=T. Counts of rRNA, and genes with 0 counts for all samples were discarded
Expression was measured as regularized-logarithm transformation (DESeq2, v1.10.1) of transcripts per kilobase million (TPM)
Genome_build: hs37d5 (ftp://ftp.1000genomes.ebi.ac.uk/vol1/ftp/technical/reference/phase2_reference_assembly_sequence/hs37d5.fa.gz)
Supplementary_files_format_and_content: read counts from data processing steps above (before DESeq application), columns represent samples, rows represent genes (Ensembl Gene ID)

molecule: polyA RNA

organism: Homo sapiens

source_name: ovarian cancer cell line UWB1.289+BRCA1

growth_protocol: UWB1.289 ovarian cancer cells: 1:1 mixture of RPMI-1640 and MEGM Bullet Kit, 10 % fetal calf serum, 100 U/ml penicillin and 100 mg/ml streptomycin; UWB1.289+BRCA1 ovarian cancer cells: 50% RPMI-1640 and 50% MEGM Bullet Kit, 10 % fetal calf serum, 100 U/ml penicillin, 100 mg/ml, streptomycin and 200 µg/ml Geneticin/G-418

extract_protocol:
RNA extraction was performed according to the miRNeasy Mini Kit (Qiagen, Hilden, Germany). On column DNA digestion was included into this protocol, in order to remove residual contaminating genomic DNA. All experiments were done according to the manufacturer’s instructions.
For library preparation we used the TruSeq Stranded mRNA Library Prep Kit (Illumina) according to the manufacturer’s protocol, starting with 1 µg total RNA. All barcoded libraries were pooled and sequenced 2x75bp paired-end on an Illumina NextSeq500 platform to obtain a minimum of 10 Mio reads per sample.

treatment_protocol: For Whole-Transcriptome Sequencing (RNA-Seq), 3.5x10E6 cells (UWB1.289) or 3.3x10E6 cells (UWB1.289+BRCA1) were seeded in T-75 flasks. After 24h, medium was removed and medium with 1000 µM CT913 and 40µM CT913-M1 or standard growth medium (as control) was added.

tag: cell line


title: R-1095_UWB1.289_40uM_CT_M1

source_name: ovarian cancer cell line UWB1.289


title: R-1098_UWB1.289+BRCA1_1000uM_CT913


title: R-1100_UWB1.289+BRCA1_40uM_CT_M1


title: R-1096_UWB1.289+BRCA1_ctr

value: control


title: R-1097_UWB1.289+BRCA1_ctr


title: R-1099_UWB1.289+BRCA1_1000uM_CT913


title: R-1094_UWB1.289_40uM_CT_M1


title: R-1093_UWB1.289_1000uM_CT913


title: R-1091_UWB1.289_ctr


title: R-1092_UWB1.289_1000uM_CT913


title: Sedentary(Control)_C15


title: R-1090_UWB1.289_ctr

source_name: ovarian cancer cell line


title: BN_MB6_F23_RNA

city: Ithaca

first: Charles

middle: G.

last: Danko

state: NY

address: Hungerford Hill Rd.

department: Baker Institute for Animal Health, College of Veterinary Medicine

country: USA

institute: Cornell University

description:
mouseID 23
BN_geneWithInATwindows_stability.txt

data_processing:
For ChRO-seq reads, adaptor sequences were trimmed with the cutadapt 1.16. PCR duplicates were removed using unique molecular identifiers (UMIs) in the sequencing data with prinseq-lite.pl. The reads were seperated with a custom J-barcode. The processed reads were then aligned with bowtie to to the diploid genome as input for AlleleDB.
The first base of nascent RNA in B6-specific, CAST-specific, or identical reads without SNPs was liftover to ref genome (mm10), and shown as bigwig files
Genome_build: mm10
Supplementary_files_format_and_content: ChRO-seq data are provided in standard bigWig file formats.

source_name: Brain

extract_protocol:
whole brain and liver were removed, flash frozen on dry ice, and 100mg of each tissue was used for a TRIzol RNA extraction. Briefly, 1 mL of TRizol was added to each sample, chloroform was used for phase separation of the aqueous phase containing RNA, RNA was precipitated using isopropanol and washed with 75% ethanol.
Purified RNA was enriched for PolyA using oligo-dT beads, and Stranded mRNA-seq libraries were prepared.

value: C57BL/6 x Castaneus F1 hybrid

value: Brain

value: post natal day 22

tag: gender

value: female


title: LV_MB6_F23_RNA

description:
mouseID 23
LV_geneWithInATwindows_stability.txt

source_name: Liver

value: Liver


title: BN_MB6_M24_RNA

description:
mouse ID 24
BN_geneWithInATwindows_stability.txt


title: LV_MB6_M24_RNA

description:
mouse ID 24
LV_geneWithInATwindows_stability.txt


title: H3K36me3_P7Wt-1_Input

city: Indianapolis

first: Nathan

middle: J

last: VanDusen

state: IN

department: Pediatrics

institute: Indiana University School of Medicine

library_source: genomic

data_processing:
Adapters were removed and reads trimmed by quality score.
Reads were aligned to the mouse genome by Bowtie2.
RPKM normalized bigwig files were generated using Deeptools.
Reads overlapping each gene body (first transcription start site to last transcription end site) were counted using Bedtools.
Counts for each gene were normalized for gene body length.
Genome_build: mm10
Supplementary_files_format_and_content:  H3K36me3_ChIP-seq.txt: tab delimited text, RPKM for each gene body. Bigwig files: RPKM normalized genome browser file for each sample.

molecule: genomic DNA

source_name: P7 ventricles

growth_protocol: In vivo growth

extract_protocol:
Ventricles homogenized at P7
KAPA Hyper Prep Kit

value: P7 ventricles

value: postnatal day 7

tag: sample  type

value: H3K36me3 Input

value: high dose AAV9-TnT-Cre


title: H3K36me3_P7Wt-2_ChIP

value: WT

value: H3K36me3 ChIP


title: H3K36me3_P7Wt-2_Input


title: nTreg KO_2

city: Boston

first: Takayuki

last: Katsuyama

state: MA

address: 3 Blackfan Circle, CLS-948

department: Rheumatology and Clinical Immunology

institute: Beth Israel Deaconess Medical Center

data_processing:
Samples were sequenced on an Illumina NextSeq500 run with single-end 75-bp reads.
Genome_build: mm9

source_name: Th0 Effector CD4 T cells

growth_protocol: T cells were isolated from spleens using pan T ell isolation kit (Miltenyi). CD4+CD25+CD127lo natural (n)Treg cells were sorted by flow cytometry.

extract_protocol:
RNA was extracted using RNeasy mini kit (Qiagen)
RNA libraries were prepared for sequencing using standard Illumina protocols

treatment_protocol: nTregs were stimulated with anti-CD3 (2 μg/ml) and anti-CD28 (2 μg/ml) antibodies and rIL-2 (10 ng/ml) for 72h

value: spleen

value: 8-10 week

value: Srsf1-flox/flox dLck.Cre


title: nTreg WT_2


title: nTreg WT_1


title: nTreg WT_3


title: nTreg KO_3


title: nTreg KO_1


title: H3K36me3_P7Wt-1_ChIP


title: H3K36me3_P7KO-2_ChIP


title: H3K4me3_P7Wt-2_Input

data_processing:
Adapters were removed and reads trimmed by quality score.
Reads were aligned to the mouse genome by Bowtie2.
RPKM normalized bigwig files were generated using Deeptools.
Read in each TSS interval were quantified using Bedtools.
Genome_build: mm10
Supplementary_files_format_and_content:  H3K4me3_ChIP-seq.txt: tab delimited text, max RPKM for each TSS interval. Bigwig files: RPKM normalized genome browser file for each sample.

value: H3K4me3 Input


title: H3K36me3_P7KO-2_Input


title: H3K4me3_P7Wt-1_Input


title: H3K36me3_P7KO-1_ChIP


title: H3K36me3_P7KO-1_Input


title: H3K4me3_P7Wt-2_ChIP

value: H3K4me3 ChIP


title: H3K4me3_P7KO-2_Input


title: H3K4me3_P7KO-2_ChIP


title: H3K4me3_P7KO-1_Input


title: H3K4me3_P7KO-1_ChIP


title: DMV neuron nucleus 380

city: Charlottesville

first: John

middle: N

last: Campbell

state: VA

address: 485 MCCORMICK RD, GILMER HALL ROOM 229 PO BOX 400328

department: Biology

institute: University of Virginia

data_processing:
sequencing reads were demultiplexed using bcl2fastq and aligned to the GRCm38 mouse genome assembly using STAR (Dobin et al., 2013)
sequencing replicates were pooled and PCR duplicates and low-quality reads were removed by Picard (https://broadinstitute.github.io/picard).
Filtered reads were assigned to the annotated transcriptome and quantiﬁed using Drop-seq tools (https://github.com/broadinstitute/Drop-seq), allowing for intronic alignments.
Normalization and differential expression analysis were performed using EdgeR (Robinson et al., 2010).
For differential gene expression analysis, we only tested genes that were detected in at least two samples with log2CPM ≥ 2. Genes were considered signiﬁcant if they passed a fold change (FC) cutoff of log2FC ≥1 and a false discovery rate (FDR) cutoff of FDR ≤ 0.05.
Nuclei were clustered in Seurat v3 (Stuart, et al. 2019); nuclei with less than 1000 unique genes expressed or with more than 10% mitochondrial content were filtered out.
Genome_build: GRCm38
Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every sample

molecule: nuclear RNA

source_name: dorsal motor nucleus of the vagus

extract_protocol:
The following morning, the RNAlater-preserved tissue samples were processed into single-nuclei suspensions according to the sNuc-Seq as previously described (Todd et al., 2020). Briefly, the pooled DMV tissue samples were dounce homogenized (Wheaton tissue grinders, catalog # 357538) and separated by density gradient centrifugation. Each pellet was then re-suspended in 0.5 ml sNuc-Seq resuspension buffer and filtered through a 20 μm mesh (Miltenyi Biotec pre-separation filters, catalog # 130-101-812) to remove clumped nuclei and larger debris. Nuclei were counterstained with two drops of NucBlue Live ReadyProbes Reagent (ThermoFisher Scientific catalog # R37605) per ml of nuclei. Nuclei were sorted by MoFlo Astrios EQ cell sorter (Harvard Bauer core facility), gating for NucBlue+/mCherry+ singlets and distributing them individually to wells of 96-well plates containing 5 μl per well of nuclei capture buffer (1% beta-mercaptoethanol in Qiagen Buffer TCL; catalog # 1031576). Each plate was loaded with single nuclei, one per well. Loaded plates were centrifuged for 5 min at 2,500 xg and 4degC to ensure nuclei reached the capture buffer and frozen at -80degC for up to 8 weeks until use.
Sample plates were transferred to an RNA-dedicated PCR cabinet, thawed quickly, and immediately purified with RNA-Clean XP (Beckman Coulter, catalog # A63987; 2.5:1 ratio of RNA-Clean XP to sample volume), eluting in 4μl Smart-seq2 buffer (2 μl Smart-seq2 cell lysis buffer, 1 μl 100 μM oligo-dT primer, and 1 μl 10 mM dNTP mix). cDNA was then generated and amplified as described in the Smart-Seq2 protocol, except that 22 PCR cycles were used for the amplification step. After the PCR step, 10 μl of each sample was transferred to new 96-well plates for subsequent processing. Amplified cDNA was purified with two rounds of SPRIselect (Beckman Coulter, catalog # B23318; 0.8:1 ratio of SPRIselect to sample volume), eluting in ultrapure water. cDNA concentration was measured by Qubit fluorometry in a microplate format, each well containing 2 μl of cDNA sample and 98 μl of Qubit working solution (ThermoFisher Scientific, catalog # Q32854). Qubit readings were collected with a fluorescence microplate reader (Molecular Devices SpectraMax M5; Harvard Medical School ICCB-Longwood core facility) and used to calculate DNA concentrations by comparing each sample’s Qubit reading to standards in a 50% dilution series (n=8 standards; range, 0.25 ng/μl to 0.01 ng/μl plus a negative control, 0.0 ng/μl). cDNA samples were then diluted to 0.15 ng/ μl with ultrapure water and processed into sequencing libraries with sample-specific Nextera indices and the Nextera DNA Sample Prep Kit and Nextera XT Index Kit v2 sets A-D (Illumina, catalog #s FC-131-1096, FC-131- 2001, FC-131-2002, FC-131-2003, FC-131-2004, respectively). Nextera XT was used at 1/4 reaction volumes but otherwise according to manufacturer’s instructions. Sequencing libraries were purified with two rounds of SPRIselect (0.8:1 ratio of SPRIselect to sample volume) and eluted in a single volume of ultrapure water. Library pool concentration was measured by Qubit according to the manufacturer’s instructions, sized using Agilent BioAnalyzer high sensitivity kit, and then diluted with ultrapure water to a size-corrected concentration of 2 nM. The diluted library pool was denatured and further diluted to 2 pM loading concentration according to Illumina NextSeq recommendations, then sequenced with a NextSeq 500 v2 75cycle sequencing kit (Illumina, catalog # FC-404-2005).
Smart-Seq2

treatment_protocol: 10 week old Chat-IRES-Cre male mice received a 100nL stereotaxic injection of AAV9-DIO-H2b-mCherry into their dorsal motor nucleus of the vagus (DMV).  Sixteen days later, they were removed from their home cage and rapidly decapitated in order to minimize stress related changes in gene expression. Brains were extracted quickly, chilled briefly in ice cold DMEM/F12 media, and then sectioned coronally at 1 mm intervals in a chilled stainless-steel brain matrix. The brain sections containing and neighboring the SCN were collected in ice-cold RNAlater (Qiagen catalog # 76106). Each section was imaged by a fluorescence stereoscope (Zeiss Discovery V8) in order to visualize fluorescently labeled cells in the DMV for dissection. Dissected tissue was pooled and placed into ice-cold RNAlater overnight in the dark at 4degC.

value: dorsal motor nucleus of the vagus

tag: cell type

value: neuron


title: DMV neuron nucleus 377


title: DMV neuron nucleus 378


title: DMV neuron nucleus 381


title: DMV neuron nucleus 384


title: DMV neuron nucleus 376


title: DMV neuron nucleus 383


title: DMV neuron nucleus 382


title: DMV neuron nucleus 370


title: DMV neuron nucleus 371


title: DMV neuron nucleus 369


title: DMV neuron nucleus 372


title: DMV neuron nucleus 373


title: DMV neuron nucleus 368


title: DMV neuron nucleus 366


title: DMV neuron nucleus 379


title: DMV neuron nucleus 375


title: DMV neuron nucleus 374


title: DMV neuron nucleus 365


title: DMV neuron nucleus 364


title: DMV neuron nucleus 360


title: DMV neuron nucleus 362


title: DMV neuron nucleus 363


title: DMV neuron nucleus 361


title: DMV neuron nucleus 352


title: DMV neuron nucleus 359


title: DMV neuron nucleus 356


title: DMV neuron nucleus 351


title: DMV neuron nucleus 353


title: DMV neuron nucleus 345


title: DMV neuron nucleus 354


title: DMV neuron nucleus 357


title: DMV neuron nucleus 358


title: DMV neuron nucleus 355


title: DMV neuron nucleus 347


title: DMV neuron nucleus 346


title: DMV neuron nucleus 343


title: DMV neuron nucleus 349


title: DMV neuron nucleus 344


title: DMV neuron nucleus 350


title: DMV neuron nucleus 348


title: DMV neuron nucleus 341


title: DMV neuron nucleus 340


title: DMV neuron nucleus 342


title: DMV neuron nucleus 312


title: DMV neuron nucleus 320


title: DMV neuron nucleus 318


title: DMV neuron nucleus 317


title: DMV neuron nucleus 321


title: DMV neuron nucleus 314


title: DMV neuron nucleus 328


title: DMV neuron nucleus 313


title: DMV neuron nucleus 316


title: DMV neuron nucleus 329


title: DMV neuron nucleus 367


title: DMV neuron nucleus 333


title: DMV neuron nucleus 325


title: DMV neuron nucleus 315


title: DMV neuron nucleus 319


title: DMV neuron nucleus 326


title: DMV neuron nucleus 336


title: DMV neuron nucleus 330


title: DMV neuron nucleus 335


title: DMV neuron nucleus 322


title: DMV neuron nucleus 334


title: DMV neuron nucleus 331


title: DMV neuron nucleus 332


title: DMV neuron nucleus 323


title: DMV neuron nucleus 311


title: DMV neuron nucleus 338


title: DMV neuron nucleus 339


title: DMV neuron nucleus 327


title: DMV neuron nucleus 337


title: DMV neuron nucleus 324


title: DMV neuron nucleus 310


title: DMV neuron nucleus 306


title: DMV neuron nucleus 305


title: DMV neuron nucleus 301


title: DMV neuron nucleus 309


title: DMV neuron nucleus 284


title: DMV neuron nucleus 285


title: DMV neuron nucleus 295


title: DMV neuron nucleus 296


title: DMV neuron nucleus 288


title: DMV neuron nucleus 282


title: DMV neuron nucleus 290


title: DMV neuron nucleus 294


title: DMV neuron nucleus 281


title: DMV neuron nucleus 297


title: DMV neuron nucleus 293


title: DMV neuron nucleus 304


title: DMV neuron nucleus 307


title: DMV neuron nucleus 300


title: DMV neuron nucleus 302


title: DMV neuron nucleus 298


title: DMV neuron nucleus 308


title: DMV neuron nucleus 283


title: DMV neuron nucleus 291


title: DMV neuron nucleus 292


title: DMV neuron nucleus 299


title: DMV neuron nucleus 289


title: DMV neuron nucleus 287


title: DMV neuron nucleus 303


title: DMV neuron nucleus 286


title: DMV neuron nucleus 280


title: DMV neuron nucleus 279


title: DMV neuron nucleus 276


title: DMV neuron nucleus 278


title: DMV neuron nucleus 270


title: DMV neuron nucleus 264


title: DMV neuron nucleus 267


title: DMV neuron nucleus 269


title: DMV neuron nucleus 273


title: DMV neuron nucleus 271


title: DMV neuron nucleus 266


title: DMV neuron nucleus 272


title: DMV neuron nucleus 263


title: DMV neuron nucleus 274


title: DMV neuron nucleus 262


title: DMV neuron nucleus 265


title: DMV neuron nucleus 256


title: DMV neuron nucleus 258


title: DMV neuron nucleus 257


title: DMV neuron nucleus 259


title: DMV neuron nucleus 254


title: DMV neuron nucleus 255


title: DMV neuron nucleus 252


title: DMV neuron nucleus 260


title: DMV neuron nucleus 275


title: DMV neuron nucleus 251


title: DMV neuron nucleus 249


title: DMV neuron nucleus 250


title: DMV neuron nucleus 253


title: DMV neuron nucleus 261


title: DMV neuron nucleus 247


title: DMV neuron nucleus 248


title: DMV neuron nucleus 244


title: DMV neuron nucleus 241


title: DMV neuron nucleus 240


title: DMV neuron nucleus 239


title: DMV neuron nucleus 242


title: DMV neuron nucleus 245


title: DMV neuron nucleus 246


title: DMV neuron nucleus 238


title: DMV neuron nucleus 234


title: DMV neuron nucleus 233


title: DMV neuron nucleus 230


title: DMV neuron nucleus 237


title: DMV neuron nucleus 235


title: DMV neuron nucleus 232


title: DMV neuron nucleus 236


title: DMV neuron nucleus 229


title: DMV neuron nucleus 228


title: DMV neuron nucleus 226


title: DMV neuron nucleus 225


title: DMV neuron nucleus 224


title: DMV neuron nucleus 243


title: DMV neuron nucleus 227


title: DMV neuron nucleus 223


title: DMV neuron nucleus 222


title: DMV neuron nucleus 215


title: DMV neuron nucleus 231


title: DMV neuron nucleus 218


title: 5d_repo_glia_P2_P9

city: Houston

first: Hongjie

last: Li

state: TX

address: one Baylor Plaza

institute: Baylor College of Medicine

description:
single_cell
in house

data_processing:
alignment (STAR 2.4.2a, ENCODE standard options, except --outFilterScoreMinOverLread 0.4 --outFilterMatchNminOverLread 0.4 --  outFilterMismatchNmax 999 --outFilterMismatchNoverLmax 0.04)
gene count (HTSeq-count 0.7.1, default options, except -m intersection-strict)
Genome_build: Dmel r6.10
Supplementary_files_format_and_content: adata_aging_glia.h5ad

organism: Drosophila melanogaster

extract_protocol:
Smart-seq2
full-length cDNA

value: repo-Gal4, GFP

value: glia

tag: number of cells

tag: orn type


title: 5d_repo_glia_P2_P8

description:
single_cell
in house


title: 5d_repo_glia_P2_P5

description:
single_cell
in house


title: DMV neuron nucleus 277


title: 5d_repo_glia_P2_P24

description:
single_cell
in house


title: 5d_repo_glia_P2_P4

description:
single_cell
in house


title: 5d_repo_glia_P2_P2

description:
single_cell
in house


title: 5d_repo_glia_P2_P3

description:
single_cell
in house


title: 5d_repo_glia_P2_P7

description:
single_cell
in house


title: 5d_repo_glia_P2_P23

description:
single_cell
in house


title: DNA w1118 female flies

city: Cambridge

first: Daniel

last: Gebert

state: Cambridgeshire

address: Downing Street

department: Department of Genetics

country: United Kingdom

institute: University of Cambridge

data_processing:
Paired-end reads were mapped to the D. melanogaster genome using BWA MEM (Li and Durbin, 2009).
The resulting SAM/BAM files were then filtered to retain only uniquely mapping reads using Samtools (Li et al., 2009) and the command line utility grep. A map quality filter with a threshold value of 60 was applied with Samtools.
Bigwig files were created with Bedtools (Quinlan and Hall, 2010) and the UCSC genome browser command-line tool bedGraphToBigWig.
Analysis of total genomic TE copy numbers was conducted by mapping paired-end reads to the complete set of D. melanogaster TE consensus sequences (FlyBase) using bowtie2 and limiting the number of distinct valid alignments to one per read (‘-k 1’). Read counts for each TE family were were multiplied by 150 nt paired-end read base pairs (300), divided by TE consensus sequence length and genomic read coverage.
dm3
bigwig files generated by BWA MEM, samtools, bedtools and bedGraphToBigWig

source_name: whole flies

extract_protocol:
Genomic DNA was obtained from 20-40 female adult flies from w1118 and a triple mutant (20A(p)∆; 38C∆, 42AB∆) stock. DNA was extracted using the Quick-DNA Microprep Kit w/ Zymo-Spin™ (Zymo Research) and quantified using Qubit (Invitrogen).
Library preparation was performed on 0.25µg of genomic DNA using the Nextera DNA Flex Library Prep kit as described by the manufacturer (Illumina).
Libraries were multiplexed and sequenced in paired-end, 150-nt-long reads on an Illumina NovaSeq.

value: whole body

value: adult

value: control


title: DNA triple mutant female flies

value: 20A(p)-delta; 38C-delta, 42AB-delta


title: 5d_repo_glia_P2_P22

description:
single_cell
in house


title: 5d_repo_glia_P2_P20

description:
single_cell
in house


title: DMV neuron nucleus 221


title: DMV neuron nucleus 220


title: 5d_repo_glia_P2_P1

description:
single_cell
in house


title: DMV neuron nucleus 219


title: DMV neuron nucleus 216


title: 5d_repo_glia_P2_P16

description:
single_cell
in house


title: 5d_repo_glia_P2_P21

description:
single_cell
in house


title: 5d_repo_glia_P2_P14

description:
single_cell
in house


title: 5d_repo_glia_P2_P19

description:
single_cell
in house


title: 5d_repo_glia_P2_P13

description:
single_cell
in house


title: DMV neuron nucleus 217


title: 5d_repo_glia_P2_P10

description:
single_cell
in house


title: 5d_repo_glia_P2_P12

description:
single_cell
in house


title: 5d_repo_glia_P2_P18

description:
single_cell
in house


title: 5d_repo_glia_P2_O7

description:
single_cell
in house


title: 5d_repo_glia_P2_O6

description:
single_cell
in house


title: 5d_repo_glia_P2_O8

description:
single_cell
in house


title: 5d_repo_glia_P2_O23

description:
single_cell
in house


title: 5d_repo_glia_P2_O24

description:
single_cell
in house


title: 5d_repo_glia_P2_O3

description:
single_cell
in house


title: 5d_repo_glia_P2_P17

description:
single_cell
in house


title: 5d_repo_glia_P2_O21

description:
single_cell
in house


title: 5d_repo_glia_P2_O2

description:
single_cell
in house


title: 5d_repo_glia_P2_O20

description:
single_cell
in house


title: 5d_repo_glia_P2_O9

description:
single_cell
in house


title: DMV neuron nucleus 268


title: 5d_repo_glia_P2_O19

description:
single_cell
in house


title: 5d_repo_glia_P2_O18

description:
single_cell
in house


title: 5d_repo_glia_P2_O1

description:
single_cell
in house


title: 5d_repo_glia_P2_P11

description:
single_cell
in house


title: 5d_repo_glia_P2_O17

description:
single_cell
in house


title: 5d_repo_glia_P2_O15

description:
single_cell
in house


title: 5d_repo_glia_P2_O14

description:
single_cell
in house


title: 5d_repo_glia_P2_O22

description:
single_cell
in house


title: 5d_repo_glia_P2_P15

description:
single_cell
in house


title: 5d_repo_glia_P2_O5

description:
single_cell
in house


title: 5d_repo_glia_P2_O16

description:
single_cell
in house


title: 5d_repo_glia_P2_O12

description:
single_cell
in house


title: 5d_repo_glia_P2_O10

description:
single_cell
in house


title: 5d_repo_glia_P2_O11

description:
single_cell
in house


title: 5d_repo_glia_P2_O4

description:
single_cell
in house


title: 5d_repo_glia_P2_N8

description:
single_cell
in house


title: 5d_repo_glia_P2_N7

description:
single_cell
in house


title: 5d_repo_glia_P2_N24

description:
single_cell
in house


title: 5d_repo_glia_P2_N9

description:
single_cell
in house


title: 5d_repo_glia_P2_N6

description:
single_cell
in house


title: 5d_repo_glia_P2_N2

description:
single_cell
in house


title: 5d_repo_glia_P2_N5

description:
single_cell
in house


title: 5d_repo_glia_P2_N23

description:
single_cell
in house


title: 5d_repo_glia_P2_N3

description:
single_cell
in house


title: 5d_repo_glia_P2_N22

description:
single_cell
in house


title: 5d_repo_glia_P2_N20

description:
single_cell
in house


title: 5d_repo_glia_P2_N21

description:
single_cell
in house


title: 5d_repo_glia_P2_O13

description:
single_cell
in house


title: 5d_repo_glia_P2_N15

description:
single_cell
in house


title: 5d_repo_glia_P2_N19

description:
single_cell
in house


title: 5d_repo_glia_P2_N17

description:
single_cell
in house


title: 5d_repo_glia_P2_N1

description:
single_cell
in house


title: 5d_repo_glia_P2_N13

description:
single_cell
in house


title: 5d_repo_glia_P2_N11

description:
single_cell
in house


title: 5d_repo_glia_P2_N18

description:
single_cell
in house


title: 5d_repo_glia_P2_N12

description:
single_cell
in house


title: 5d_repo_glia_P2_N16

description:
single_cell
in house


title: 5d_repo_glia_P2_M8

description:
single_cell
in house


title: 5d_repo_glia_P2_M6

description:
single_cell
in house


title: 5d_repo_glia_P2_N10

description:
single_cell
in house


title: 5d_repo_glia_P2_N14

description:
single_cell
in house


title: 5d_repo_glia_P2_N4

description:
single_cell
in house


title: 5d_repo_glia_P2_M5

description:
single_cell
in house


title: 5d_repo_glia_P2_M2

description:
single_cell
in house


title: 5d_repo_glia_P2_M23

description:
single_cell
in house


title: 5d_repo_glia_P2_M22

description:
single_cell
in house


title: 5d_repo_glia_P2_M21

description:
single_cell
in house


title: 5d_repo_glia_P2_M24

description:
single_cell
in house


title: 5d_repo_glia_P2_M1

description:
single_cell
in house


title: 5d_repo_glia_P2_M19

description:
single_cell
in house


title: 5d_repo_glia_P2_M18

description:
single_cell
in house


title: 5d_repo_glia_P2_M9

description:
single_cell
in house


title: 5d_repo_glia_P2_M7

description:
single_cell
in house


title: 5d_repo_glia_P2_M14

description:
single_cell
in house


title: 5d_repo_glia_P2_M11

description:
single_cell
in house


title: 5d_repo_glia_P2_M16

description:
single_cell
in house


title: 5d_repo_glia_P2_M12

description:
single_cell
in house


title: 5d_repo_glia_P2_M15

description:
single_cell
in house


title: 5d_repo_glia_P2_L9

description:
single_cell
in house


title: 5d_repo_glia_P2_M17

description:
single_cell
in house


title: 5d_repo_glia_P2_L8

description:
single_cell
in house


title: 5d_repo_glia_P2_L5

description:
single_cell
in house


title: 5d_repo_glia_P2_L7

description:
single_cell
in house


title: 5d_repo_glia_P2_M4

description:
single_cell
in house


title: 5d_repo_glia_P2_L4

description:
single_cell
in house


title: 5d_repo_glia_P2_L3

description:
single_cell
in house


title: 5d_repo_glia_P2_L2

description:
single_cell
in house


title: 5d_repo_glia_P2_M20

description:
single_cell
in house


title: 5d_repo_glia_P2_L24

description:
single_cell
in house


title: 5d_repo_glia_P2_L22

description:
single_cell
in house


title: 5d_repo_glia_P2_P6

description:
single_cell
in house


title: 5d_repo_glia_P2_L20

description:
single_cell
in house


title: 5d_repo_glia_P2_L1

description:
single_cell
in house


title: 5d_repo_glia_P2_L19

description:
single_cell
in house


title: 5d_repo_glia_P2_M3

description:
single_cell
in house


title: 5d_repo_glia_P2_L17

description:
single_cell
in house


title: 5d_repo_glia_P2_M10

description:
single_cell
in house


title: 5d_repo_glia_P2_M13

description:
single_cell
in house


title: 5d_repo_glia_P2_L14

description:
single_cell
in house


title: 5d_repo_glia_P2_L13

description:
single_cell
in house


title: 5d_repo_glia_P2_L15

description:
single_cell
in house


title: 5d_repo_glia_P2_L16

description:
single_cell
in house


title: 5d_repo_glia_P2_K9

description:
single_cell
in house


title: 5d_repo_glia_P2_L10

description:
single_cell
in house


title: 5d_repo_glia_P2_L12

description:
single_cell
in house


title: 5d_repo_glia_P2_K5

description:
single_cell
in house


title: 5d_repo_glia_P2_K8

description:
single_cell
in house


title: 5d_repo_glia_P2_K7

description:
single_cell
in house


title: 5d_repo_glia_P2_L11

description:
single_cell
in house


title: 5d_repo_glia_P2_K3

description:
single_cell
in house


title: 5d_repo_glia_P2_K6

description:
single_cell
in house


title: 5d_repo_glia_P2_K2

description:
single_cell
in house


title: 5d_repo_glia_P2_K24

description:
single_cell
in house


title: 5d_repo_glia_P2_L21

description:
single_cell
in house


title: 5d_repo_glia_P2_K23

description:
single_cell
in house


title: 5d_repo_glia_P2_K22

description:
single_cell
in house


title: 5d_repo_glia_P2_K4

description:
single_cell
in house


title: 5d_repo_glia_P2_K20

description:
single_cell
in house


title: 5d_repo_glia_P2_K21

description:
single_cell
in house


title: 5d_repo_glia_P2_K18

description:
single_cell
in house


title: 5d_repo_glia_P2_K17

description:
single_cell
in house


title: 5d_repo_glia_P2_L18

description:
single_cell
in house


title: 5d_repo_glia_P2_K16

description:
single_cell
in house


title: 5d_repo_glia_P2_K14

description:
single_cell
in house


title: 5d_repo_glia_P2_K11

description:
single_cell
in house


title: 5d_repo_glia_P2_K15

description:
single_cell
in house


title: 5d_repo_glia_P2_K13

description:
single_cell
in house


title: 5d_repo_glia_P2_J7

description:
single_cell
in house


title: 5d_repo_glia_P2_K10

description:
single_cell
in house


title: 5d_repo_glia_P2_J5

description:
single_cell
in house


title: 5d_repo_glia_P2_J6

description:
single_cell
in house


title: 5d_repo_glia_P2_J3

description:
single_cell
in house


title: 5d_repo_glia_P2_J8

description:
single_cell
in house


title: 5d_repo_glia_P2_J9

description:
single_cell
in house


title: 5d_repo_glia_P2_J4

description:
single_cell
in house


title: 5d_repo_glia_P2_J24

description:
single_cell
in house


title: 5d_repo_glia_P2_J21

description:
single_cell
in house


title: 5d_repo_glia_P2_K1

description:
single_cell
in house


title: 5d_repo_glia_P2_J1

description:
single_cell
in house


title: 5d_repo_glia_P2_J20

description:
single_cell
in house


title: 5d_repo_glia_P2_J2

description:
single_cell
in house


title: 5d_repo_glia_P2_J23

description:
single_cell
in house


title: 5d_repo_glia_P2_J22

description:
single_cell
in house


title: 5d_repo_glia_P2_K19

description:
single_cell
in house


title: 5d_repo_glia_P2_J18

description:
single_cell
in house


title: 5d_repo_glia_P2_J16

description:
single_cell
in house


title: 5d_repo_glia_P2_J15

description:
single_cell
in house


title: 5d_repo_glia_P2_J17

description:
single_cell
in house


title: 5d_repo_glia_P2_J13

description:
single_cell
in house


title: 5d_repo_glia_P2_I7

description:
single_cell
in house


title: 5d_repo_glia_P2_J14

description:
single_cell
in house


title: 5d_repo_glia_P2_I5

description:
single_cell
in house


title: 5d_repo_glia_P2_I6

description:
single_cell
in house


title: 5d_repo_glia_P2_I8

description:
single_cell
in house


title: 5d_repo_glia_P2_J11

description:
single_cell
in house


title: 5d_repo_glia_P2_L23

description:
single_cell
in house


title: 5d_repo_glia_P2_J10

description:
single_cell
in house


title: 5d_repo_glia_P2_I4

description:
single_cell
in house


title: 5d_repo_glia_P2_J12

description:
single_cell
in house


title: 5d_repo_glia_P2_I9

description:
single_cell
in house


title: 5d_repo_glia_P2_I2

description:
single_cell
in house


title: 5d_repo_glia_P2_I23

description:
single_cell
in house


title: 5d_repo_glia_P2_I22

description:
single_cell
in house


title: 5d_repo_glia_P2_I3

description:
single_cell
in house


title: 5d_repo_glia_P2_I21

description:
single_cell
in house


title: 5d_repo_glia_P2_I18

description:
single_cell
in house


title: 5d_repo_glia_P2_I19

description:
single_cell
in house


title: 5d_repo_glia_P2_I17

description:
single_cell
in house


title: 5d_repo_glia_P2_I20

description:
single_cell
in house


title: 5d_repo_glia_P2_I15

description:
single_cell
in house


title: 5d_repo_glia_P2_I13

description:
single_cell
in house


title: 5d_repo_glia_P2_H7

description:
single_cell
in house


title: 5d_repo_glia_P2_I12

description:
single_cell
in house


title: 5d_repo_glia_P2_H6

description:
single_cell
in house


title: 5d_repo_glia_P2_I14

description:
single_cell
in house


title: 5d_repo_glia_P2_H4

description:
single_cell
in house


title: 5d_repo_glia_P2_I24

description:
single_cell
in house


title: 5d_repo_glia_P2_H9

description:
single_cell
in house


title: 5d_repo_glia_P2_I10

description:
single_cell
in house


title: 5d_repo_glia_P2_K12

description:
single_cell
in house


title: 5d_repo_glia_P2_H8

description:
single_cell
in house


title: 5d_repo_glia_P2_H5

description:
single_cell
in house


title: 5d_repo_glia_P2_I1

description:
single_cell
in house


title: 5d_repo_glia_P2_I16

description:
single_cell
in house


title: 5d_repo_glia_P2_H21

description:
single_cell
in house


title: 5d_repo_glia_P2_H3

description:
single_cell
in house


title: 5d_repo_glia_P2_H2

description:
single_cell
in house


title: 5d_repo_glia_P2_H19

description:
single_cell
in house


title: 5d_repo_glia_P2_L6

description:
single_cell
in house


title: 5d_repo_glia_P2_H18

description:
single_cell
in house


title: 5d_repo_glia_P2_H17

description:
single_cell
in house


title: 5d_repo_glia_P2_H23

description:
single_cell
in house


title: 5d_repo_glia_P2_H22

description:
single_cell
in house


title: 5d_repo_glia_P2_H15

description:
single_cell
in house


title: 5d_repo_glia_P2_H20

description:
single_cell
in house


title: 5d_repo_glia_P2_H1

description:
single_cell
in house


title: 5d_repo_glia_P2_H14

description:
single_cell
in house


title: 5d_repo_glia_P2_J19

description:
single_cell
in house


title: 5d_repo_glia_P2_I11

description:
single_cell
in house


title: 5d_repo_glia_P2_H24

description:
single_cell
in house


title: 5d_repo_glia_P2_H11

description:
single_cell
in house


title: 5d_repo_glia_P2_G9

description:
single_cell
in house


title: 5d_repo_glia_P2_H13

description:
single_cell
in house


title: 5d_repo_glia_P2_H10

description:
single_cell
in house


title: 5d_repo_glia_P2_G7

description:
single_cell
in house


title: 5d_repo_glia_P2_G8

description:
single_cell
in house


title: 5d_repo_glia_P2_G6

description:
single_cell
in house


title: 5d_repo_glia_P2_G3

description:
single_cell
in house


title: 5d_repo_glia_P2_G2

description:
single_cell
in house


title: 5d_repo_glia_P2_G24

description:
single_cell
in house


title: 5d_repo_glia_P2_G4

description:
single_cell
in house


title: 5d_repo_glia_P2_H12

description:
single_cell
in house


title: 5d_repo_glia_P2_G23

description:
single_cell
in house


title: 5d_repo_glia_P2_G21

description:
single_cell
in house


title: 5d_repo_glia_P2_G17

description:
single_cell
in house


title: 5d_repo_glia_P2_G16

description:
single_cell
in house


title: 5d_repo_glia_P2_G15

description:
single_cell
in house


title: 5d_repo_glia_P2_G20

description:
single_cell
in house


title: 5d_repo_glia_P2_H16

description:
single_cell
in house


title: 5d_repo_glia_P2_G13

description:
single_cell
in house


title: 5d_repo_glia_P2_G14

description:
single_cell
in house


title: 5d_repo_glia_P2_G1

description:
single_cell
in house


title: 5d_repo_glia_P2_G19

description:
single_cell
in house


title: 5d_repo_glia_P2_G12

description:
single_cell
in house


title: 5d_repo_glia_P2_G10

description:
single_cell
in house


title: 5d_repo_glia_P2_G5

description:
single_cell
in house


title: 5d_repo_glia_P2_F9

description:
single_cell
in house


title: 5d_repo_glia_P2_F4

description:
single_cell
in house


title: 5d_repo_glia_P2_F8

description:
single_cell
in house


title: 5d_repo_glia_P2_F5

description:
single_cell
in house


title: 5d_repo_glia_P2_F3

description:
single_cell
in house


title: 5d_repo_glia_P2_F7

description:
single_cell
in house


title: 5d_repo_glia_P2_G18

description:
single_cell
in house


title: 5d_repo_glia_P2_G22

description:
single_cell
in house


title: 5d_repo_glia_P2_F2

description:
single_cell
in house


title: 5d_repo_glia_P2_F24

description:
single_cell
in house


title: 5d_repo_glia_P2_F20

description:
single_cell
in house


title: 5d_repo_glia_P2_F1

description:
single_cell
in house


title: 5d_repo_glia_P2_F23

description:
single_cell
in house


title: 5d_repo_glia_P2_F19

description:
single_cell
in house


title: 5d_repo_glia_P2_G11

description:
single_cell
in house


title: 5d_repo_glia_P2_F16

description:
single_cell
in house


title: 5d_repo_glia_P2_F15

description:
single_cell
in house


title: 5d_repo_glia_P2_F17

description:
single_cell
in house


title: 5d_repo_glia_P2_F18

description:
single_cell
in house


title: 5d_repo_glia_P2_F12

description:
single_cell
in house


title: 5d_repo_glia_P2_F10

description:
single_cell
in house


title: 5d_repo_glia_P2_E7

description:
single_cell
in house


title: 5d_repo_glia_P2_F11

description:
single_cell
in house


title: 5d_repo_glia_P2_F13

description:
single_cell
in house


title: 5d_repo_glia_P2_E9

description:
single_cell
in house


title: 5d_repo_glia_P2_F14

description:
single_cell
in house


title: 5d_repo_glia_P2_E6

description:
single_cell
in house


title: 5d_repo_glia_P2_E2

description:
single_cell
in house


title: 5d_repo_glia_P2_E4

description:
single_cell
in house


title: 5d_repo_glia_P2_E3

description:
single_cell
in house


title: 5d_repo_glia_P2_F21

description:
single_cell
in house


title: 5d_repo_glia_P2_E8

description:
single_cell
in house


title: 5d_repo_glia_P2_F22

description:
single_cell
in house


title: 5d_repo_glia_P2_E20

description:
single_cell
in house


title: 5d_repo_glia_P2_E21

description:
single_cell
in house


title: 5d_repo_glia_P2_E1

description:
single_cell
in house


title: 5d_repo_glia_P2_E5

description:
single_cell
in house


title: 5d_repo_glia_P2_E23

description:
single_cell
in house


title: 5d_repo_glia_P2_E19

description:
single_cell
in house


title: 5d_repo_glia_P2_E22

description:
single_cell
in house


title: 5d_repo_glia_P2_E17

description:
single_cell
in house


title: 5d_repo_glia_P2_E16

description:
single_cell
in house


title: 5d_repo_glia_P2_E15

description:
single_cell
in house


title: 5d_repo_glia_P2_E14

description:
single_cell
in house


title: 5d_repo_glia_P2_E12

description:
single_cell
in house


title: 5d_repo_glia_P2_E18

description:
single_cell
in house


title: 5d_repo_glia_P2_E13

description:
single_cell
in house


title: 5d_repo_glia_P2_D5

description:
single_cell
in house


title: 5d_repo_glia_P2_E24

description:
single_cell
in house


title: 5d_repo_glia_P2_E11

description:
single_cell
in house


title: 5d_repo_glia_P2_E10

description:
single_cell
in house


title: 5d_repo_glia_P2_D6

description:
single_cell
in house


title: 5d_repo_glia_P2_D8

description:
single_cell
in house


title: 5d_repo_glia_P2_F6

description:
single_cell
in house


title: 5d_repo_glia_P2_D4

description:
single_cell
in house


title: 5d_repo_glia_P2_D9

description:
single_cell
in house


title: 5d_repo_glia_P2_D3

description:
single_cell
in house


title: 5d_repo_glia_P2_D24

description:
single_cell
in house


title: 5d_repo_glia_P2_D22

description:
single_cell
in house


title: 5d_repo_glia_P2_D23

description:
single_cell
in house


title: 5d_repo_glia_P2_D1

description:
single_cell
in house


title: 5d_repo_glia_P2_D2

description:
single_cell
in house


title: 5d_repo_glia_P2_D20

description:
single_cell
in house


title: 5d_repo_glia_P2_D16

description:
single_cell
in house


title: 5d_repo_glia_P2_D19

description:
single_cell
in house


title: 5d_repo_glia_P2_D18

description:
single_cell
in house


title: 5d_repo_glia_P2_D14

description:
single_cell
in house


title: 5d_repo_glia_P2_D13

description:
single_cell
in house


title: 5d_repo_glia_P2_D12

description:
single_cell
in house


title: 5d_repo_glia_P2_D17

description:
single_cell
in house


title: 5d_repo_glia_P2_D11

description:
single_cell
in house


title: 5d_repo_glia_P2_D10

description:
single_cell
in house


title: 5d_repo_glia_P2_D7

description:
single_cell
in house


title: 5d_repo_glia_P2_C8

description:
single_cell
in house


title: 5d_repo_glia_P2_C6

description:
single_cell
in house


title: 5d_repo_glia_P2_C4

description:
single_cell
in house


title: 5d_repo_glia_P2_C24

description:
single_cell
in house


title: 5d_repo_glia_P2_C23

description:
single_cell
in house


title: 5d_repo_glia_P2_C5

description:
single_cell
in house


title: 5d_repo_glia_P2_C22

description:
single_cell
in house


title: 5d_repo_glia_P2_C21

description:
single_cell
in house


title: 5d_repo_glia_P2_C20

description:
single_cell
in house


title: 5d_repo_glia_P2_C3

description:
single_cell
in house


title: 5d_repo_glia_P2_C19

description:
single_cell
in house


title: 5d_repo_glia_P2_D15

description:
single_cell
in house


title: 5d_repo_glia_P2_C16

description:
single_cell
in house


title: 5d_repo_glia_P2_C14

description:
single_cell
in house


title: 5d_repo_glia_P2_C18

description:
single_cell
in house


title: 5d_repo_glia_P2_C12

description:
single_cell
in house


title: 5d_repo_glia_P2_C13

description:
single_cell
in house


title: 5d_repo_glia_P2_C10

description:
single_cell
in house


title: 5d_repo_glia_P2_B9

description:
single_cell
in house


title: 5d_repo_glia_P2_C9

description:
single_cell
in house


title: 5d_repo_glia_P2_B8

description:
single_cell
in house


title: 5d_repo_glia_P2_B7

description:
single_cell
in house


title: 5d_repo_glia_P2_B6

description:
single_cell
in house


title: 5d_repo_glia_P2_B5

description:
single_cell
in house


title: 5d_repo_glia_P2_C7

description:
single_cell
in house


title: 5d_repo_glia_P2_B3

description:
single_cell
in house


title: 5d_repo_glia_P2_C2

description:
single_cell
in house


title: 5d_repo_glia_P2_B4

description:
single_cell
in house


title: 5d_repo_glia_P2_C1

description:
single_cell
in house


title: 5d_repo_glia_P2_B23

description:
single_cell
in house


title: 5d_repo_glia_P2_B2

description:
single_cell
in house


title: 5d_repo_glia_P2_B22

description:
single_cell
in house


title: 5d_repo_glia_P2_B20

description:
single_cell
in house


title: 5d_repo_glia_P2_B21

description:
single_cell
in house


title: 5d_repo_glia_P2_B1

description:
single_cell
in house


title: 5d_repo_glia_P2_B19

description:
single_cell
in house


title: 5d_repo_glia_P2_B18

description:
single_cell
in house


title: 5d_repo_glia_P2_B17

description:
single_cell
in house


title: 5d_repo_glia_P2_C15

description:
single_cell
in house


title: 5d_repo_glia_P2_C11

description:
single_cell
in house


title: 5d_repo_glia_P2_B16

description:
single_cell
in house


title: 5d_repo_glia_P2_B14

description:
single_cell
in house


title: 5d_repo_glia_P2_B15

description:
single_cell
in house


title: 5d_repo_glia_P2_B11

description:
single_cell
in house


title: 5d_repo_glia_P2_B12

description:
single_cell
in house


title: 5d_repo_glia_P2_B13

description:
single_cell
in house


title: 5d_repo_glia_P2_B10

description:
single_cell
in house


title: 5d_repo_glia_P2_A8

description:
single_cell
in house


title: 5d_repo_glia_P2_A7

description:
single_cell
in house


title: 5d_repo_glia_P2_A4

description:
single_cell
in house


title: 5d_repo_glia_P2_A3

description:
single_cell
in house


title: 5d_repo_glia_P2_A9

description:
single_cell
in house


title: 5d_repo_glia_P2_B24

description:
single_cell
in house


title: 5d_repo_glia_P2_A2

description:
single_cell
in house


title: 5d_repo_glia_P2_A24

description:
single_cell
in house


title: 5d_repo_glia_P2_A22

description:
single_cell
in house


title: 5d_repo_glia_P2_A23

description:
single_cell
in house


title: 5d_repo_glia_P2_A20

description:
single_cell
in house


title: 5d_repo_glia_P2_A21

description:
single_cell
in house


title: 5d_repo_glia_P2_A1

description:
single_cell
in house


title: 5d_repo_glia_P2_A19

description:
single_cell
in house


title: 5d_repo_glia_P2_A17

description:
single_cell
in house


title: 5d_repo_glia_P2_A15

description:
single_cell
in house


title: 5d_repo_glia_P2_A14

description:
single_cell
in house


title: 5d_repo_glia_P2_A18

description:
single_cell
in house


title: 5d_repo_glia_P2_A11

description:
single_cell
in house


title: 5d_repo_glia_P2_A13

description:
single_cell
in house


title: 5d_repo_glia_P1_P6

description:
single_cell
in house


title: 5d_repo_glia_P1_P8

description:
single_cell
in house


title: 5d_repo_glia_P2_A6

description:
single_cell
in house


title: 5d_repo_glia_P1_P7

description:
single_cell
in house


title: 5d_repo_glia_P2_A5

description:
single_cell
in house


title: 5d_repo_glia_P2_A12

description:
single_cell
in house


title: 5d_repo_glia_P1_P5

description:
single_cell
in house


title: 5d_repo_glia_P1_P4

description:
single_cell
in house


title: 5d_repo_glia_P2_A10

description:
single_cell
in house


title: 5d_repo_glia_P1_P2

description:
single_cell
in house


title: 5d_repo_glia_P1_P3

description:
single_cell
in house


title: 5d_repo_glia_P1_P24

description:
single_cell
in house


title: 5d_repo_glia_P1_P22

description:
single_cell
in house


title: 5d_repo_glia_P1_P21

description:
single_cell
in house


title: 5d_repo_glia_P1_P20

description:
single_cell
in house


title: 5d_repo_glia_P1_P1

description:
single_cell
in house


title: 5d_repo_glia_P1_P17

description:
single_cell
in house


title: 5d_repo_glia_P1_P19

description:
single_cell
in house


title: 5d_repo_glia_P1_P18

description:
single_cell
in house


title: 5d_repo_glia_P1_P9

description:
single_cell
in house


title: 5d_repo_glia_P2_A16

description:
single_cell
in house


title: 5d_repo_glia_P1_P12

description:
single_cell
in house


title: 5d_repo_glia_P1_P11

description:
single_cell
in house


title: 5d_repo_glia_P1_P10

description:
single_cell
in house


title: 5d_repo_glia_P1_O9

description:
single_cell
in house


title: 5d_repo_glia_P1_O8

description:
single_cell
in house


title: 5d_repo_glia_P1_O6

description:
single_cell
in house


title: 5d_repo_glia_P1_O7

description:
single_cell
in house


title: 5d_repo_glia_P1_O3

description:
single_cell
in house


title: 5d_repo_glia_P1_O23

description:
single_cell
in house


title: 5d_repo_glia_P1_O4

description:
single_cell
in house


title: 5d_repo_glia_P1_O2

description:
single_cell
in house


title: 5d_repo_glia_P1_P23

description:
single_cell
in house


title: 5d_repo_glia_P1_O21

description:
single_cell
in house


title: 5d_repo_glia_P1_O19

description:
single_cell
in house


title: 5d_repo_glia_P1_O24

description:
single_cell
in house


title: 5d_repo_glia_P1_O1

description:
single_cell
in house


title: 5d_repo_glia_P1_O20

description:
single_cell
in house


title: 5d_repo_glia_P1_P14

description:
single_cell
in house


title: 5d_repo_glia_P1_P16

description:
single_cell
in house


title: 5d_repo_glia_P1_O18

description:
single_cell
in house


title: 5d_repo_glia_P1_O17

description:
single_cell
in house


title: 5d_repo_glia_P1_O16

description:
single_cell
in house


title: 5d_repo_glia_P1_O15

description:
single_cell
in house


title: 5d_repo_glia_P1_P13

description:
single_cell
in house


title: 5d_repo_glia_P1_P15

description:
single_cell
in house


title: 5d_repo_glia_P1_O14

description:
single_cell
in house


title: 5d_repo_glia_P1_O12

description:
single_cell
in house


title: 5d_repo_glia_P1_O10

description:
single_cell
in house


title: 5d_repo_glia_P1_O11

description:
single_cell
in house


title: 5d_repo_glia_P1_N9

description:
single_cell
in house


title: 5d_repo_glia_P1_O13

description:
single_cell
in house


title: 5d_repo_glia_P1_O5

description:
single_cell
in house


title: 5d_repo_glia_P1_N8

description:
single_cell
in house


title: 5d_repo_glia_P1_O22

description:
single_cell
in house


title: 5d_repo_glia_P1_N5

description:
single_cell
in house


title: 5d_repo_glia_P1_N6

description:
single_cell
in house


title: 5d_repo_glia_P1_N7

description:
single_cell
in house


title: 5d_repo_glia_P1_N4

description:
single_cell
in house


title: 5d_repo_glia_P1_N3

description:
single_cell
in house


title: 5d_repo_glia_P1_N24

description:
single_cell
in house


title: 5d_repo_glia_P1_N2

description:
single_cell
in house


title: 5d_repo_glia_P1_N20

description:
single_cell
in house


title: 5d_repo_glia_P1_N22

description:
single_cell
in house


title: 5d_repo_glia_P1_N23

description:
single_cell
in house


title: 5d_repo_glia_P1_N21

description:
single_cell
in house


title: 5d_repo_glia_P1_N1

description:
single_cell
in house


title: 5d_repo_glia_P1_N19

description:
single_cell
in house


title: 5d_repo_glia_P1_N17

description:
single_cell
in house


title: 5d_repo_glia_P1_N12

description:
single_cell
in house


title: 5d_repo_glia_P1_N18

description:
single_cell
in house


title: 5d_repo_glia_P1_N10

description:
single_cell
in house


title: 5d_repo_glia_P1_N11

description:
single_cell
in house


title: 5d_repo_glia_P1_N15

description:
single_cell
in house


title: 5d_repo_glia_P1_N16

description:
single_cell
in house


title: 5d_repo_glia_P1_N13

description:
single_cell
in house


title: 5d_repo_glia_P1_M5

description:
single_cell
in house


title: 5d_repo_glia_P1_M23

description:
single_cell
in house


title: 5d_repo_glia_P1_M4

description:
single_cell
in house


title: 5d_repo_glia_P1_M2

description:
single_cell
in house


title: 5d_repo_glia_P1_M9

description:
single_cell
in house


title: 5d_repo_glia_P1_M1

description:
single_cell
in house


title: 5d_repo_glia_P1_M3

description:
single_cell
in house


title: 5d_repo_glia_P1_M19

description:
single_cell
in house


title: 5d_repo_glia_P1_M6

description:
single_cell
in house


title: 5d_repo_glia_P1_M22

description:
single_cell
in house


title: 5d_repo_glia_P1_M8

description:
single_cell
in house


title: 5d_repo_glia_P1_M20

description:
single_cell
in house


title: 5d_repo_glia_P1_M7

description:
single_cell
in house


title: 5d_repo_glia_P1_M18

description:
single_cell
in house


title: 5d_repo_glia_P1_M21

description:
single_cell
in house


title: 5d_repo_glia_P1_M17

description:
single_cell
in house


title: 5d_repo_glia_P1_M16

description:
single_cell
in house


title: 5d_repo_glia_P1_M13

description:
single_cell
in house


title: 5d_repo_glia_P1_M15

description:
single_cell
in house


title: 5d_repo_glia_P1_M14

description:
single_cell
in house


title: 5d_repo_glia_P1_N14

description:
single_cell
in house


title: 5d_repo_glia_P1_M11

description:
single_cell
in house


title: 5d_repo_glia_P1_M12

description:
single_cell
in house


title: 5d_repo_glia_P1_L5

description:
single_cell
in house


title: 5d_repo_glia_P1_L8

description:
single_cell
in house


title: 5d_repo_glia_P1_L6

description:
single_cell
in house


title: 5d_repo_glia_P1_L9

description:
single_cell
in house


title: 5d_repo_glia_P1_M10

description:
single_cell
in house


title: 5d_repo_glia_P1_M24

description:
single_cell
in house


title: 5d_repo_glia_P1_L4

description:
single_cell
in house


title: 5d_repo_glia_P1_L24

description:
single_cell
in house


title: 5d_repo_glia_P1_L3

description:
single_cell
in house


title: 5d_repo_glia_P1_L15

description:
single_cell
in house


title: 5d_repo_glia_P1_L1

description:
single_cell
in house


title: 5d_repo_glia_P1_L12

description:
single_cell
in house


title: 5d_repo_glia_P1_L17

description:
single_cell
in house


title: 5d_repo_glia_P1_L18

description:
single_cell
in house


title: 5d_repo_glia_P1_L7

description:
single_cell
in house


title: 5d_repo_glia_P1_L13

description:
single_cell
in house


title: 5d_repo_glia_P1_L19

description:
single_cell
in house


title: 5d_repo_glia_P1_L16

description:
single_cell
in house


title: 5d_repo_glia_P1_L22

description:
single_cell
in house


title: 5d_repo_glia_P1_L20

description:
single_cell
in house


title: 5d_repo_glia_P1_L14

description:
single_cell
in house


title: 5d_repo_glia_P1_L21

description:
single_cell
in house


title: 5d_repo_glia_P1_K6

description:
single_cell
in house


title: 5d_repo_glia_P1_K7

description:
single_cell
in house


title: 5d_repo_glia_P1_L2

description:
single_cell
in house


title: 5d_repo_glia_P1_K5

description:
single_cell
in house


title: 5d_repo_glia_P1_K3

description:
single_cell
in house


title: 5d_repo_glia_P1_L11

description:
single_cell
in house


title: 5d_repo_glia_P1_K9

description:
single_cell
in house


title: 5d_repo_glia_P1_K24

description:
single_cell
in house


title: 5d_repo_glia_P1_K4

description:
single_cell
in house


title: 5d_repo_glia_P1_K8

description:
single_cell
in house


title: 5d_repo_glia_P1_K23

description:
single_cell
in house


title: 5d_repo_glia_P1_L23

description:
single_cell
in house


title: 5d_repo_glia_P1_K2

description:
single_cell
in house


title: 5d_repo_glia_P1_K22

description:
single_cell
in house


title: 5d_repo_glia_P1_K21

description:
single_cell
in house


title: 5d_repo_glia_P1_K20

description:
single_cell
in house


title: 5d_repo_glia_P1_K1

description:
single_cell
in house


title: 5d_repo_glia_P1_K18

description:
single_cell
in house


title: 5d_repo_glia_P1_K16

description:
single_cell
in house


title: 5d_repo_glia_P1_K15

description:
single_cell
in house


title: 5d_repo_glia_P1_K10

description:
single_cell
in house


title: 5d_repo_glia_P1_K19

description:
single_cell
in house


title: 5d_repo_glia_P1_J9

description:
single_cell
in house


title: 5d_repo_glia_P1_K14

description:
single_cell
in house


title: 5d_repo_glia_P1_K13

description:
single_cell
in house


title: 5d_repo_glia_P1_L10

description:
single_cell
in house


title: 5d_repo_glia_P1_K11

description:
single_cell
in house


title: 5d_repo_glia_P1_J8

description:
single_cell
in house


title: 5d_repo_glia_P1_J6

description:
single_cell
in house


title: 5d_repo_glia_P1_J7

description:
single_cell
in house


title: 5d_repo_glia_P1_J4

description:
single_cell
in house


title: 5d_repo_glia_P1_J2

description:
single_cell
in house


title: 5d_repo_glia_P1_J20

description:
single_cell
in house


title: 5d_repo_glia_P1_J5

description:
single_cell
in house


title: 5d_repo_glia_P1_J22

description:
single_cell
in house


title: 5d_repo_glia_P1_J24

description:
single_cell
in house


title: 5d_repo_glia_P1_J23

description:
single_cell
in house


title: 5d_repo_glia_P1_J3

description:
single_cell
in house


title: 5d_repo_glia_P1_J21

description:
single_cell
in house


title: 5d_repo_glia_P1_J17

description:
single_cell
in house


title: 5d_repo_glia_P1_J16

description:
single_cell
in house


title: 5d_repo_glia_P1_J12

description:
single_cell
in house


title: 5d_repo_glia_P1_K17

description:
single_cell
in house


title: 5d_repo_glia_P1_J10

description:
single_cell
in house


title: 5d_repo_glia_P1_J14

description:
single_cell
in house


title: 5d_repo_glia_P1_J11

description:
single_cell
in house


title: 5d_repo_glia_P1_J15

description:
single_cell
in house


title: 5d_repo_glia_P1_I9

description:
single_cell
in house


title: 5d_repo_glia_P1_J13

description:
single_cell
in house


title: 5d_repo_glia_P1_I8

description:
single_cell
in house


title: 5d_repo_glia_P1_K12

description:
single_cell
in house


title: 5d_repo_glia_P1_I4

description:
single_cell
in house


title: 5d_repo_glia_P1_I6

description:
single_cell
in house


title: 5d_repo_glia_P1_J18

description:
single_cell
in house


title: 5d_repo_glia_P1_J19

description:
single_cell
in house


title: 5d_repo_glia_P1_I3

description:
single_cell
in house


title: 5d_repo_glia_P1_I21

description:
single_cell
in house


title: 5d_repo_glia_P1_I13

description:
single_cell
in house


title: 5d_repo_glia_P1_I20

description:
single_cell
in house


title: 5d_repo_glia_P1_I18

description:
single_cell
in house


title: 5d_repo_glia_P1_I1

description:
single_cell
in house


title: 5d_repo_glia_P1_I15

description:
single_cell
in house


title: 5d_repo_glia_P1_I12

description:
single_cell
in house


title: 5d_repo_glia_P1_I14

description:
single_cell
in house


title: 5d_repo_glia_P1_I16

description:
single_cell
in house


title: 5d_repo_glia_P1_I24

description:
single_cell
in house


title: 5d_repo_glia_P1_I23

description:
single_cell
in house


title: 5d_repo_glia_P1_I22

description:
single_cell
in house


title: 5d_repo_glia_P1_H8

description:
single_cell
in house


title: 5d_repo_glia_P1_I11

description:
single_cell
in house


title: 5d_repo_glia_P1_I17

description:
single_cell
in house


title: 5d_repo_glia_P1_H6

description:
single_cell
in house


title: 5d_repo_glia_P1_H4

description:
single_cell
in house


title: 5d_repo_glia_P1_H7

description:
single_cell
in house


title: 5d_repo_glia_P1_H5

description:
single_cell
in house


title: 5d_repo_glia_P1_H2

description:
single_cell
in house


title: 5d_repo_glia_P1_H3

description:
single_cell
in house


title: 5d_repo_glia_P1_H22

description:
single_cell
in house


title: 5d_repo_glia_P1_H23

description:
single_cell
in house


title: 5d_repo_glia_P1_I7

description:
single_cell
in house


title: 5d_repo_glia_P1_I19

description:
single_cell
in house


title: 5d_repo_glia_P1_I2

description:
single_cell
in house


title: 5d_repo_glia_P1_I10

description:
single_cell
in house


title: 5d_repo_glia_P1_H9

description:
single_cell
in house


title: 5d_repo_glia_P1_H21

description:
single_cell
in house


title: 5d_repo_glia_P1_H13

description:
single_cell
in house


title: 5d_repo_glia_P1_H14

description:
single_cell
in house


title: 5d_repo_glia_P1_H18

description:
single_cell
in house


title: 5d_repo_glia_P1_H17

description:
single_cell
in house


title: 5d_repo_glia_P1_G8

description:
single_cell
in house


title: 5d_repo_glia_P1_H20

description:
single_cell
in house


title: 5d_repo_glia_P1_G6

description:
single_cell
in house


title: 5d_repo_glia_P1_H1

description:
single_cell
in house


title: 5d_repo_glia_P1_H16

description:
single_cell
in house


title: 5d_repo_glia_P1_H19

description:
single_cell
in house


title: 5d_repo_glia_P1_H24

description:
single_cell
in house


title: 5d_repo_glia_P1_G5

description:
single_cell
in house


title: 5d_repo_glia_P1_H12

description:
single_cell
in house


title: 5d_repo_glia_P1_G4

description:
single_cell
in house


title: 5d_repo_glia_P1_H11

description:
single_cell
in house


title: 5d_repo_glia_P1_G9

description:
single_cell
in house


title: 5d_repo_glia_P1_G3

description:
single_cell
in house


title: 5d_repo_glia_P1_H10

description:
single_cell
in house


title: 5d_repo_glia_P1_G23

description:
single_cell
in house


title: 5d_repo_glia_P1_G24

description:
single_cell
in house


title: 5d_repo_glia_P1_G7

description:
single_cell
in house


title: 5d_repo_glia_P1_G2

description:
single_cell
in house


title: 5d_repo_glia_P1_G22

description:
single_cell
in house


title: 5d_repo_glia_P1_G21

description:
single_cell
in house


title: 5d_repo_glia_P1_G1

description:
single_cell
in house


title: 5d_repo_glia_P1_G19

description:
single_cell
in house


title: 5d_repo_glia_P1_G20

description:
single_cell
in house


title: 5d_repo_glia_P1_G18

description:
single_cell
in house


title: 5d_repo_glia_P1_H15

description:
single_cell
in house


title: 5d_repo_glia_P1_G17

description:
single_cell
in house


title: 5d_repo_glia_P1_G16

description:
single_cell
in house


title: 5d_repo_glia_P1_G12

description:
single_cell
in house


title: 5d_repo_glia_P1_G14

description:
single_cell
in house


title: 5d_repo_glia_P1_F8

description:
single_cell
in house


title: 5d_repo_glia_P1_F5

description:
single_cell
in house


title: 5d_repo_glia_P1_F4

description:
single_cell
in house


title: 5d_repo_glia_P1_F7

description:
single_cell
in house


title: 5d_repo_glia_P1_G11

description:
single_cell
in house


title: 5d_repo_glia_P1_F23

description:
single_cell
in house


title: 5d_repo_glia_P1_F3

description:
single_cell
in house


title: 5d_repo_glia_P1_F6

description:
single_cell
in house


title: 5d_repo_glia_P1_F2

description:
single_cell
in house


title: 5d_repo_glia_P1_G15

description:
single_cell
in house


title: 5d_repo_glia_P1_G13

description:
single_cell
in house


title: 5d_repo_glia_P1_F21

description:
single_cell
in house


title: 5d_repo_glia_P1_F17

description:
single_cell
in house


title: 5d_repo_glia_P1_F24

description:
single_cell
in house


title: 5d_repo_glia_P1_F9

description:
single_cell
in house


title: 5d_repo_glia_P1_F19

description:
single_cell
in house


title: 5d_repo_glia_P1_F18

description:
single_cell
in house


title: 5d_repo_glia_P1_F15

description:
single_cell
in house


title: 5d_repo_glia_P1_F16

description:
single_cell
in house


title: 5d_repo_glia_P1_F14

description:
single_cell
in house


title: 5d_repo_glia_P1_F20

description:
single_cell
in house


title: 5d_repo_glia_P1_F1

description:
single_cell
in house


title: 5d_repo_glia_P1_F12

description:
single_cell
in house


title: 5d_repo_glia_P1_G10

description:
single_cell
in house


title: 5d_repo_glia_P1_F11

description:
single_cell
in house


title: 5d_repo_glia_P1_F22

description:
single_cell
in house


title: 5d_repo_glia_P1_E8

description:
single_cell
in house


title: 5d_repo_glia_P1_E9

description:
single_cell
in house


title: 5d_repo_glia_P1_E7

description:
single_cell
in house


title: 5d_repo_glia_P1_E5

description:
single_cell
in house


title: 5d_repo_glia_P1_E2

description:
single_cell
in house


title: 5d_repo_glia_P1_E4

description:
single_cell
in house


title: 5d_repo_glia_P1_E22

description:
single_cell
in house


title: 5d_repo_glia_P1_E20

description:
single_cell
in house


title: 5d_repo_glia_P1_E21

description:
single_cell
in house


title: 5d_repo_glia_P1_E24

description:
single_cell
in house


title: 5d_repo_glia_P1_E23

description:
single_cell
in house


title: 5d_repo_glia_P1_E17

description:
single_cell
in house


title: 5d_repo_glia_P1_E15

description:
single_cell
in house


title: 5d_repo_glia_P1_E1

description:
single_cell
in house


title: 5d_repo_glia_P1_E19

description:
single_cell
in house


title: 5d_repo_glia_P1_E13

description:
single_cell
in house


title: 5d_repo_glia_P1_F13

description:
single_cell
in house


title: 5d_repo_glia_P1_E16

description:
single_cell
in house


title: 5d_repo_glia_P1_E11

description:
single_cell
in house


title: 5d_repo_glia_P1_E10

description:
single_cell
in house


title: 5d_repo_glia_P1_E12

description:
single_cell
in house


title: 5d_repo_glia_P1_E14

description:
single_cell
in house


title: 5d_repo_glia_P1_D9

description:
single_cell
in house


title: 5d_repo_glia_P1_D8

description:
single_cell
in house


title: 5d_repo_glia_P1_F10

description:
single_cell
in house


title: 5d_repo_glia_P1_D6

description:
single_cell
in house


title: 5d_repo_glia_P1_D7

description:
single_cell
in house


title: 5d_repo_glia_P1_E6

description:
single_cell
in house


title: 5d_repo_glia_P1_D3

description:
single_cell
in house


title: 5d_repo_glia_P1_E3

description:
single_cell
in house


title: 5d_repo_glia_P1_D2

description:
single_cell
in house


title: 5d_repo_glia_P1_E18

description:
single_cell
in house


title: 5d_repo_glia_P1_D23

description:
single_cell
in house


title: 5d_repo_glia_P1_D24

description:
single_cell
in house


title: 5d_repo_glia_P1_D22

description:
single_cell
in house


title: 5d_repo_glia_P1_D4

description:
single_cell
in house


title: 5d_repo_glia_P1_D20

description:
single_cell
in house


title: 5d_repo_glia_P1_D21

description:
single_cell
in house


title: 5d_repo_glia_P1_D18

description:
single_cell
in house


title: 5d_repo_glia_P1_D13

description:
single_cell
in house


title: 5d_repo_glia_P1_D16

description:
single_cell
in house


title: 5d_repo_glia_P1_D15

description:
single_cell
in house


title: 5d_repo_glia_P1_D11

description:
single_cell
in house


title: 5d_repo_glia_P1_D19

description:
single_cell
in house


title: 5d_repo_glia_P1_D17

description:
single_cell
in house


title: 5d_repo_glia_P1_D12

description:
single_cell
in house


title: 5d_repo_glia_P1_C7

description:
single_cell
in house


title: 5d_repo_glia_P1_C6

description:
single_cell
in house


title: 5d_repo_glia_P1_D10

description:
single_cell
in house


title: 5d_repo_glia_P1_C9

description:
single_cell
in house


title: 5d_repo_glia_P1_D5

description:
single_cell
in house


title: 5d_repo_glia_P1_C2

description:
single_cell
in house


title: 5d_repo_glia_P1_C3

description:
single_cell
in house


title: 5d_repo_glia_P1_C5

description:
single_cell
in house


title: 5d_repo_glia_P1_C4

description:
single_cell
in house


title: 5d_repo_glia_P1_C24

description:
single_cell
in house


title: 5d_repo_glia_P1_D1

description:
single_cell
in house


title: 5d_repo_glia_P1_C22

description:
single_cell
in house


title: 5d_repo_glia_P1_C23

description:
single_cell
in house


title: 5d_repo_glia_P1_C21

description:
single_cell
in house


title: 5d_repo_glia_P1_D14

description:
single_cell
in house


title: 5d_repo_glia_P1_C19

description:
single_cell
in house


title: 5d_repo_glia_P1_C20

description:
single_cell
in house


title: 5d_repo_glia_P1_C18

description:
single_cell
in house


title: 5d_repo_glia_P1_C8

description:
single_cell
in house


title: 5d_repo_glia_P1_C15

description:
single_cell
in house


title: 5d_repo_glia_P1_C14

description:
single_cell
in house


title: 5d_repo_glia_P1_C16

description:
single_cell
in house


title: 5d_repo_glia_P1_C12

description:
single_cell
in house


title: 5d_repo_glia_P1_B9

description:
single_cell
in house


title: 5d_repo_glia_P1_C13

description:
single_cell
in house


title: 5d_repo_glia_P1_C11

description:
single_cell
in house


title: 5d_repo_glia_P1_B6

description:
single_cell
in house


title: 5d_repo_glia_P1_C10

description:
single_cell
in house


title: 5d_repo_glia_P1_B4

description:
single_cell
in house


title: 5d_repo_glia_P1_B3

description:
single_cell
in house


title: 5d_repo_glia_P1_B8

description:
single_cell
in house


title: 5d_repo_glia_P1_B7

description:
single_cell
in house


title: 5d_repo_glia_P1_B5

description:
single_cell
in house


title: 5d_repo_glia_P1_B24

description:
single_cell
in house


title: 5d_repo_glia_P1_B2

description:
single_cell
in house


title: 5d_repo_glia_P1_B23

description:
single_cell
in house


title: 5d_repo_glia_P1_C1

description:
single_cell
in house


title: 5d_repo_glia_P1_B22

description:
single_cell
in house


title: 5d_repo_glia_P1_C17

description:
single_cell
in house


title: 5d_repo_glia_P1_B21

description:
single_cell
in house


title: 5d_repo_glia_P1_B20

description:
single_cell
in house


title: 5d_repo_glia_P1_B19

description:
single_cell
in house


title: 5d_repo_glia_P1_B18

description:
single_cell
in house


title: 5d_repo_glia_P1_B17

description:
single_cell
in house


title: 5d_repo_glia_P1_B15

description:
single_cell
in house


title: 5d_repo_glia_P1_B14

description:
single_cell
in house


title: 5d_repo_glia_P1_B13

description:
single_cell
in house


title: 5d_repo_glia_P1_B11

description:
single_cell
in house


title: 5d_repo_glia_P1_A8

description:
single_cell
in house


title: 5d_repo_glia_P1_A9

description:
single_cell
in house


title: 5d_repo_glia_P1_A7

description:
single_cell
in house


title: 5d_repo_glia_P1_B10

description:
single_cell
in house


title: 5d_repo_glia_P1_A4

description:
single_cell
in house


title: 5d_repo_glia_P1_A5

description:
single_cell
in house


title: 5d_repo_glia_P1_A6

description:
single_cell
in house


title: 5d_repo_glia_P1_A2

description:
single_cell
in house


title: 5d_repo_glia_P1_A23

description:
single_cell
in house


title: 5d_repo_glia_P1_A20

description:
single_cell
in house


title: 5d_repo_glia_P1_A21

description:
single_cell
in house


title: 5d_repo_glia_P1_A1

description:
single_cell
in house


title: 5d_repo_glia_P1_B1

description:
single_cell
in house


title: 5d_repo_glia_P1_A18

description:
single_cell
in house


title: 5d_repo_glia_P1_A17

description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house

value: GMR56F03-Gal4, GFP


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description:
single_cell
in house


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single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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single_cell
in house


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single_cell
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description:
single_cell
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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
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single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
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single_cell
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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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description:
single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
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single_cell
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single_cell
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single_cell
in house


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single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
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single_cell
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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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single_cell
in house


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single_cell
in house


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description:
single_cell
in house


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single_cell
in house


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description:
single_cell
in house


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description:
single_cell
in house


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single_cell
in house


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description:
single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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description:
single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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description:
single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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description:
single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
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single_cell
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single_cell
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single_cell
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single_cell
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single_cell
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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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description:
single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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description:
single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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description:
single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house


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single_cell
in house