title: MM_M1_2_sample 2884


title: MM_M1_2_sample 2880

status: Public on Oct 06 2021

type: SRA

city: La Jolla

first: Joseph

middle: R

last: Ecker

state: CA

address: 10010 North Torrey Pines Road

department: Genomic Analysis Laboratory

country: USA

institute: HHMI-Salk-Institute

library_source: genomic

data_processing:
Library strategy: snmC-seq2
Reads trimed with Cutadapt for adapters and low quality bases (Phred score >= 20)
The resulting files are mapped to in-silico bisulfite converted reference genome using Bismark.
The raw BAM file will be filtered using samtools to filter reads by MAPQ > 10 and use Picard to remove PCR duplicates
Tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls will be generated with in-house developing package using the filtered BAM files
Genome_build: ASM275486v1
Supplementary_files_format_and_content: tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls. Columns are: 1. Chromosome, 2. Position, 3. Strand, 4. Cytosin Context, 5. methylated base call, 6. total mapped base call, 7. not used, place holder column

molecule: genomic DNA

organism: Callithrix jacchus

extract_protocol:
M1 regions of human/marmoset brains were dissected.
Library prepared following protocol discribed in Luo, Chongyuan, Angeline Rivkin, Jingtian Zhou, Justin P. Sandoval, Laurie Kurihara, Jacinta Lucero, Rosa Castanon, et al. 2018. “Robust Single-Cell DNA Methylome Profiling with snmC-seq2.” Nature Communications 9 (1): 3824.

tag: tissue

value: brain

tag: brain region

tag: gender

value: M


title: MM_M1_2_sample 2877


title: MM_M1_2_sample 2882


title: HS_M1_2_sample 2911

data_processing:
Library strategy: snmC-seq2
Reads trimed with Cutadapt for adapters and low quality bases (Phred score >= 20)
The resulting files are mapped to in-silico bisulfite converted reference genome using Bismark.
The raw BAM file will be filtered using samtools to filter reads by MAPQ > 10 and use Picard to remove PCR duplicates
Tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls will be generated with in-house developing package using the filtered BAM files
Genome_build: hg19
Supplementary_files_format_and_content: tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls. Columns are: 1. Chromosome, 2. Position, 3. Strand, 4. Cytosin Context, 5. methylated base call, 6. total mapped base call, 7. not used, place holder column

organism: Homo sapiens

value: F


title: HS_M1_2_sample 2910


title: HS_M1_2_sample 2912


title: HS_M1_2_sample 2915


title: HS_M1_2_sample 2908


title: MM_M1_2_sample 2876


title: HS_M1_2_sample 2909


title: HS_M1_2_sample 2905


title: HS_M1_2_sample 2904


title: MM_M1_2_sample 2875


title: MM_M1_2_sample 2870


title: MM_M1_2_sample 2873


title: MM_M1_2_sample 2879


title: MM_M1_2_sample 2874


title: MM_M1_2_sample 2871


title: MM_M1_2_sample 2867


title: MM_M1_2_sample 2866


title: HS_M1_2_sample 2921


title: MM_M1_2_sample 2878


title: HS_M1_2_sample 2902


title: HS_M1_2_sample 2907


title: MM_M1_2_sample 2864


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title: HS_M1_2_sample 2898


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title: MM_M1_2_sample 2869


title: HS_M1_2_sample 2894


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title: HS_M1_2_sample 2896


title: MM_M1_2_sample 2859


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title: HS_M1_2_sample 2881


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title: MM_M1_2_sample 2800


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title: MM_M1_2_sample 2801


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title: HS_M1_2_sample 2808


title: MM_M1_2_sample 2786


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title: MM_M1_2_sample 2776


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title: MM_M1_2_sample 2030


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title: MM_M1_2_sample 2027


title: MM_M1_2_sample 2023


title: HS_M1_2_sample 2055


title: MM_M1_2_sample 2022


title: MM_M1_2_sample 2026


title: MM_M1_2_sample 2020


title: HS_M1_2_sample 2056


title: HS_M1_2_sample 2057


title: MM_M1_2_sample 2018


title: MM_M1_2_sample 2017


title: MM_M1_2_sample 2016


title: HS_M1_2_sample 2049


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title: MM_M1_2_sample 2015


title: MM_M1_2_sample 2014


title: HS_M1_2_sample 2048


title: MM_M1_2_sample 2019


title: MM_M1_2_sample 2011


title: HS_M1_2_sample 2053


title: MM_M1_2_sample 2021


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title: HS_M1_2_sample 2042


title: MM_M1_2_sample 2010


title: HS_M1_2_sample 2040


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title: MM_M1_2_sample 2006


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title: HS_M1_2_sample 2046


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title: MM_M1_2_sample 2013


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title: HS_M1_2_sample 2061


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title: HS_M1_2_sample 2028


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title: MM_M1_2_sample 1989


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title: HS_M1_2_sample 2019


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title: HS_M1_2_sample 2016


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title: HS_M1_2_sample 2018


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