title: A1bVav2ary-WT3

value: Arid1b +/+ VavCre+ MLL-AF9 bone marrow leukemia cells


title: A1bVav2ary-WT1

status: Public on Mar 08 2022

type: SRA

city: Milwaukee

last: Zhu

state: WI

address: 8733 W Watertown Plank Rd

country: USA

institute: Versiti Blood Research Institute Milwaukee

library_source: transcriptomic

data_processing:
Data were demultiplexed using Illumina BaseSpace
Reads were quality controlled, aligned, and expression was quantified using the BasePairTech.com "Expression Count (STAR)" pipeline.
Genome_build: mm10
Supplementary_files_format_and_content: CSV of unnormalized readcounts per gene, per sample.

molecule: polyA RNA

organism: Mus musculus

source_name: mouse bone marrow

extract_protocol:
Total RNA was extracted with TRIzol reagent (Thermo Fisher Scientific, Waltham, MA ). mRNA was further extracted using NEBNext Poly(a) mRNA Magnetic Isolation Module (New England BioLabs Inc., Ipswitch, MA).
cDNA Libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs Inc., Ipswitch, MA).

treatment_protocol: 21 days post transplantation mice receiving MxCre+ (control), Arid1b fl/fl MxCre+ (knockout), and Arid2 fl/fl MxCre+ (knockout) MLL-AF9 cells were injected with pIpC: HMW (12.5μg/gram body weight, 3 times over a one week period) (Invivogen, San Diego, CA). Bone marrow was isolated from moribund mice.

tag: cell type


title: A1bVav2ary-WT2


title: MY-R26

city: Stanford

first: Gabor

last: Boross

state: CA

address: 450 Serra Mall

institute: Stanford University

description:
total RNA was extracted from isolated cancer cells with rRNA depletion and high-throughput DNA sequenced
20210101_Oncogene_Negative_RNAseq_DESeq2_Counts.csv & 20210101_Oncogene_Negative_RNAseq_TPM.csv

data_processing:
For genomic barcoded DNA, the sgIDs (specific for given guideRNA and so genotype of the tumor) and barcodes (specific to tumor) were identified from fastq files and tumor size (cell number) was calculated by normalizing the number of reads to  three benchmarks “spike-in” cell lines with custom R and python scripts.
Supplementary_files_format_and_content: The oncogene_negative_LUAD_tumor_sizes.csv file contains all tumor sizes (number of cells) for all tumors/expansions. The experiment, mouse genotype, drug treatment (if not applicable: "Untreated), sample title, guideRNA are shown in comma separated text table. For samples without "spike-in" instead of tumor cell number, read count is shown for each tumor.
RNA-Seq reads were aligned to the mouse genome (mm10) using 2-Pass mapping with STAR v2.6.1 and standard input parameters (sjdbOverhang of 75 bp) (Dobin et al., 2013). Estimates of transcript abundance were generated using RSEM v1.2.3 (Li et al. 2011) and imported using tximport (Soneson et al., 2015) for differential expression analysis with DESeq2 v1.26.0 (Love et al., 2014).
Genome_build: mm10
Supplementary_files_format_and_content: Supplementary files include raw gene-level counts exported from DESeq2 following import with tximport (20210101_Oncogene_Negative_RNAseq_DESeq2_Counts.csv) and TPM-normalized expression (20210101_Oncogene_Negative_RNAseq_TPM.csv).

molecule: total RNA

source_name: isolated cancer cells from autochthonous tumors

growth_protocol: Autochthonous lung tumors were initiated in mice below. Lentiviral vectors and time of tissue harvest are indicated in front of each group:  • RosaLSL-tdTomato;H11LSL-Cas9 (TC) with Lenti-sgNf1-sgRasa1-sgPten/Cre lentiviral vector, harvested 4 months after tumor initiation   • KrasLSL-G12D;TC with Lenti-sgInert/Cre lentiviral vector, harvested 10 months after tumor initiation  • KrasLSL-G12D;TC with Lenti-sgPten/Cre lentiviral vector, harvested 4 months after tumor initiation

extract_protocol:
Primary tumors were dissociated using collagenase IV, dispase, and trypsin at 37 °C for 30 min. After dissociation, the samples remained continually on ice, were in contact with ice-cold solutions, and were in the presence of 2 mM EDTA and 1 U/ml DNase to prevent aggregation. Cells were stained with antibodies to CD45 (BioLegened, 103112), CD31 (BioLegend, 303116), F4/80 (BioLegend, 123116), and Ter119 (BioLegend, 116212) to exclude hematopoietic and endothelial cells (lineage-positive (Lin+) cells). DAPI was used to exclude dead cells. FACSAria sorters (BD Biosciences) were used for cell sorting.  RNA was purified using RNA/DNA All Prep kit (Qiagen, 80284). RNA quality of each tumor sample was assessed using the RNA6000 PicoAssay for the Agilent 2100 Bioanalyzer as per the manufacturer’s recommendation.
4.4 ng total RNA per sample was used for cDNA synthesis and library preparation using Trio RNA-Seq, Mouse rRNA kit (Tecan, 0507-32), according to the manufacturer’s instructions. The purified cDNA library products were evaluated using the Agilent bioanalyzer and sequenced on NextSeq High Output 1x75 (Admera Health Biopharma Services).

treatment_protocol: some of TC mice were treated with 30 mg/kg RMC-4550 and 100 mg/kg capivasertib for 3 days

tag: experiment

value: RNA-seq

tag: genotype

value: KTC

tag: drug treatment

value: Untreated

tag: molecule subtype

value: total RNA


title: MY-R30


title: MY-R25


title: MY-R23


title: 2-MxA2-WT-2aryMA9

value: Arid1b +/+ MxCre+ MLL-AF9 bone marrow leukemia cells


title: MY-R29


title: MY-R24


title: MY-R9

value: TC

value: RMC-4550 and Capivasertib (RC)


title: MY-R27


title: MY-R3

value: Vehicle


title: MY-R28


title: MY-R2


title: MY-R5


title: MY-R1


title: MY-R7


title: MY-R8


title: IW3929

description:
barcode region of lentiviral backbones from genomic DNA from bulk tumor-bearing lungs were PCR-amplified and high-throughput DNA sequenced
oncogene_negative_LUAD_tumor_sizes.csv

library_source: genomic

data_processing:
Library strategy: amplicon-seq
For genomic barcoded DNA, the sgIDs (specific for given guideRNA and so genotype of the tumor) and barcodes (specific to tumor) were identified from fastq files and tumor size (cell number) was calculated by normalizing the number of reads to  three benchmarks “spike-in” cell lines with custom R and python scripts.
Supplementary_files_format_and_content: The oncogene_negative_LUAD_tumor_sizes.csv file contains all tumor sizes (number of cells) for all tumors/expansions. The experiment, mouse genotype, drug treatment (if not applicable: "Untreated), sample title, guideRNA are shown in comma separated text table. For samples without "spike-in" instead of tumor cell number, read count is shown for each tumor.
RNA-Seq reads were aligned to the mouse genome (mm10) using 2-Pass mapping with STAR v2.6.1 and standard input parameters (sjdbOverhang of 75 bp) (Dobin et al., 2013). Estimates of transcript abundance were generated using RSEM v1.2.3 (Li et al. 2011) and imported using tximport (Soneson et al., 2015) for differential expression analysis with DESeq2 v1.26.0 (Love et al., 2014).
Genome_build: mm10
Supplementary_files_format_and_content: Supplementary files include raw gene-level counts exported from DESeq2 following import with tximport (20210101_Oncogene_Negative_RNAseq_DESeq2_Counts.csv) and TPM-normalized expression (20210101_Oncogene_Negative_RNAseq_TPM.csv).

molecule: genomic DNA

source_name: lung

extract_protocol:
For DNA extraction from single dissected tumors to generate libraries for Tuba-seq, we used Qiagen AllPrep DNA/RNA Micro kit. For bulk lung samples, following homogenization and overnight protease K digestion, genomicDNA was extracted from the lung lysates using standard phenol-chloroform and ethanolprecipitation methods.
Q5 High-Fidelity 2x Master Mix (New England Biolabs, M0494X) was used to amplify the sgID-BC region from 50 ng of DNA from dissected tumors or 32 μg of bulk lung genomic DNA. The unique dual-indexed primers used were Forward: AATGATACGGCGACCACCGAGATCTACAC- 8 nucleotides for i5 index-ACACTCTTTCCCTACACGACGCTCTTCCGATCT-6 to 9 random nucleotides for increasing the diversity-GCGCACGTCTGCCGCGCTG and Reverse: CAAGCAGAAGACGGCATACGAGAT-6 nucleotides for i7 index-GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT-9 to 6 random nucleotides for increasing the diversity-CAGGTTCTTGCGAACCTCAT. The PCR products were purified with Agencourt AMPure XP beads (Beckman Coulter, A63881) using a double size selection protocol. The concentration and quality of the purified libraries were determined using the Agilent High Sensitivity DNA kit (Agilent Technologies, 5067-121791801811821831841851861871881891901911921931941951961974626) on the Agilent 2100 Bioanalyzer (Agilent Technologies, G2939BA). The libraries were pooled based on lung weights to ensure even reading depth, cleaned up again using AMPure XP beads, and sequenced (read length 2x150bp) on the Illumina HiSeq 2500 or NextSeq 500 platform (Admera Health Biopharma Services).

value: KT

value: genomic barcoded DNA


title: IW3910


title: IW3920


title: IW3940


title: MY1936


title: IW3909


title: IW3941


title: IW3942


title: MY1935


title: MY1941


title: MY1966


title: MY1965


title: MY1949


title: MY1950


title: MY1926


title: MY1934


title: IW3243

value: Capivasertib (C )


title: MY1940

value: Trametinib (T)


title: MY1969


title: MY1946


title: MY1927


title: MY1933


title: MY1952


title: MY1947


title: MY1945


title: MY1938


title: MY2000


title: MY1943


title: MY1939


title: MY1997


title: SC3873


title: SC3872


title: MY1937


title: MY1984


title: IW3250


title: CM2496


title: MY1983


title: IW3248


title: IW3246


title: IW3242


title: MY1998


title: MY1996


title: SC3874


title: MY907


title: MY909


title: MY915


title: MY913


title: MY1992


title: MY912


title: CM2492


title: CM2371


title: MY908


title: MY910


title: MY911


title: CM2370


title: MY914


title: MY916


title: MT667


title: MT626


title: MT617


title: MY1991


title: MT671


title: MT673


title: MY1990


title: ChIP_N20_K30_r1

city: Bayreuth

first: Andreas

last: Pittroff

address: Universitätsstr. 30

department: BIOmac Research Center, NW1

country: Germany

institute: University of Bayreuth

description: CHIPSEQ

data_processing:
For GRO-Seq, the raw fastq reads were trimmed using cutadapt with parameters -a AAAAAAAAAAAAAAAAAAAA -z -e 0.10 -f fastq -m 32 (Chae et al., 2015).
The trimmed FASTQ reads were aligned to UCSC's mm10 genome using Bowtie2 with default parameters (Langmead and Salzberg, 2012). Reads with mapping quality less than 10 were removed using SAMtools (Li et al., 2009). To normalize the differences in sequencing depths, the mapped reads were “down sampled” to the lowest number of the uniquely mapped reads with duplicates followed by duplicate reads removal using ‘Sambamba’ (Tarasov et al., 2015). The bigWig files were generated using ‘bamCoverage’ included in ‘Deeptools’ package for visualization on UCSC genome browser. The coverage values in bigWig files were normalized to RPGC (Reads per genomic content).
RPKM calculations were done using the HOMER package by normalizing to 10 million reads
Tracks for visualization on UCSC browser are generated using HOMER.
Genome_build: mm10
Supplementary_files_format_and_content: Strand specific bigWig files for visualizing expression levels on a genome browser.

source_name: Cortical neurons

growth_protocol: Primary cortical neurons from E16/18 mice were cultured in Neurobasal media with B-27 and Glutamax until DIV7

extract_protocol:
RNA was extracted using acid phenol for GRO-seq. Protein was UV-crosslinked with RNA, cross-linked samples incubated with antibodies, and RNA was purified for eCLIP-seq. Protein was crosslinked with DNA, cross-linked samples incubated with antibodies, and genomic DNA was purified for ChIP-seq.
GRO-seq libraries was prepared as previously described (C. G. Danko, Mol Cell 50, 212-222, 2013). eCLIP-seq libraries was prepared as previously described (E. L. Van Nostrand, Nat Methods 13(6):508-14, 2016). ChIP-seq libraries were prepared using NEBNext ChIP-Seq Library Prep Master Mix Set (NEB).

treatment_protocol: For KCl depolarization, neurons at DIV 7 were made quiescent by 1 μM tetrodotoxin (TTX) overnight and then added with 55 mM KCl for the indicated length of time.

tag: strain

value: Cortical neurons

tag: developmental stage

tag: treatment

value: TTX/ KCl

tag: antibody

value: Pol II (N-20) (Santa Cruz Biotech; sc-899X)


title: MT669


title: MT670


title: CM2499


title: MT624


title: ChIP_N20_Un_r1

description: CHIPSEQ

value: TTX


title: MT668


title: ChIP_NELFE_TTX_K30_r3

description: CHIPSEQ

value: NELF-E (Abcam; ab170104)


title: ChIP_N20_K60_r1

description: CHIPSEQ


title: ChIP_NELFE_TTX_Un_r3

description: CHIPSEQ


title: MT672


title: ChIP_NelfE_TTX_Input_rep2

description: CHIPSEQ


title: ChIP_N20_K10_r1

description: CHIPSEQ


title: ChIP_NELFE_TTX_K10_r3

description: CHIPSEQ


title: eClip_K30_UV_N_E

description: eCLIPSEQ


title: ChIP_NelfE_TTX_KCl_30m_rep2

description: CHIPSEQ


title: eClip_K30_UV_N_E_SDS 1%

description: eCLIPSEQ


title: eClip_K10_UV_N_E_SDS 1%

description: eCLIPSEQ


title: eClip_Un_UV_N_E

description: eCLIPSEQ


title: ChIP_NelfE_TTX_ctr_rep2

description: CHIPSEQ


title: eClip_K10_UV_N_E

description: eCLIPSEQ


title: ChIP_IN_Un_r1

description: CHIPSEQ


title: eClip_Un_UV_N_E_SDS 1%

description: eCLIPSEQ


title: GRO_TTX_KCl_60M_REP4

description:
nascent RNA
GROSEQ

data_processing:
Library strategy: GRO-Seq
For GRO-Seq, the raw fastq reads were trimmed using cutadapt with parameters -a AAAAAAAAAAAAAAAAAAAA -z -e 0.10 -f fastq -m 32 (Chae et al., 2015).
The trimmed FASTQ reads were aligned to UCSC's mm10 genome using Bowtie2 with default parameters (Langmead and Salzberg, 2012). Reads with mapping quality less than 10 were removed using SAMtools (Li et al., 2009). To normalize the differences in sequencing depths, the mapped reads were “down sampled” to the lowest number of the uniquely mapped reads with duplicates followed by duplicate reads removal using ‘Sambamba’ (Tarasov et al., 2015). The bigWig files were generated using ‘bamCoverage’ included in ‘Deeptools’ package for visualization on UCSC genome browser. The coverage values in bigWig files were normalized to RPGC (Reads per genomic content).
RPKM calculations were done using the HOMER package by normalizing to 10 million reads
Tracks for visualization on UCSC browser are generated using HOMER.
Genome_build: mm10
Supplementary_files_format_and_content: Strand specific bigWig files for visualizing expression levels on a genome browser.


title: GRO_TTX_KCl_30M_REP5

description:
nascent RNA
GROSEQ


title: Input_NELFE_r3

description: CHIPSEQ


title: GRO_TTX_KCl_10M_REP5

description:
nascent RNA
GROSEQ


title: GRO_TTX_KCl_60M_REP5

description:
nascent RNA
GROSEQ


title: GRO_TTX_KCl_10M_REP4

description:
nascent RNA
GROSEQ


title: 1M7_modified_Run2

description:
SHAPE reactivities for all eRNAs from this run are concatenated in shape_reactivities txt file
shape_reactivities_Run2.txt

data_processing:
Library strategy: SHAPE-MaP
RTA2 was used for base-calling on NextSeq 500
SHAPE-MaP sequencing reads were analyzed by the ShapeMapper2 tool (Busan and Weeks, 2018) using default parameters, thus aligning the reads to the given eRNA references via bowtie2.
Supplementary_files_format_and_content: txt files contain the calculated SHAPE reactivies (from ShapeMapper2). The 4 columns contain eRNA-ID, Nucleotide Position, SHAPE Reactivity & Sequence. eRNA results are concatenated.

molecule: other

source_name: in vitro transcription

growth_protocol: mouse eRNA sequences were cloned into pUC18 vectors containing a T7 promoter sequence (5'-TAATACGACTCACTATAGG) directly in front of the eRNA sequence. The PCR templates were generated using T7 promoter spanning forward primer and an eRNA specific reverse primer. Transcribed eRNAs were purified by Urea-PAGE, passively eluted into RNA-buffer and concentrated to a volume of 0.5-1 ml. eRNAs were then subjected to size exclusion chromatography.

extract_protocol: The libraries were constructured using the SHAPE-MaP small RNA workflow  protocol (Smola et al., 2015) with minor alternations. The eRNAs were cleaned up after chemical treatment with 1.8x AmpureXP beads  (Beckman Coulter) according to the manufacturer’s instructions. The modified eRNA was subjected to reverse transcription using a specific SHAPE-MaP RT primer complementary to the 3' linker region. The produced cDNA was cleaned up with 1.8x AmpureXP beads and was amplified in a first PCR using partial Illumina primer and second PCR using the RP1 and two different Index-primers (Illumina)for DMSO control and the 1M7 modified libraries. The DNA from the 1stPCR was purified with 1.0x AmpureXP beads and from the 2nd with 0.8x. Final libraries were checked on a fragment analyzer and pooled equimolar before sequencing.

treatment_protocol: 40 eRNAs (1-200nt) were produced as described above, except that eRNA fragments were flanked with 3’ & 5’ linkers. Each purified eRNA was incubated in a folding buffer for 30min at 37°C and was split into two samples. Each sample was treated either with 1μL of 100% DMSO (DMSO control sample) or 1μL of 100mM 1-methyl-7-nitroisatoic anhydride (1M7 modified sample) at 37°C for 5min. The treatment was repeated for a second round of modification to achieve higher modification rates.

tag: run


title: GRO_TTX_CTR_REP4

description:
nascent RNA
GROSEQ


title: 1M7_modified_Run1

description:
SHAPE reactivities for all eRNAs from this run are concatenated in shape_reactivities txt file
shape_reactivities_Run1.txt


title: GRO_TTX_KCl_30M_REP4

description:
nascent RNA
GROSEQ


title: DMSO_Control_Run2

value: DMSO


title: DMSO_Control_Run1


title: ChIP_NelfE_TTX_KCl_10m_rep2

description: CHIPSEQ


title: ExoSeq_Control

data_processing:
library strategy: 5'-ExoSeq
RTA2 was used for base-calling on NextSeq 500
5’ ExoSeq reads were aligned to mm10 using STAR (Dobin et al., 2013; version 2.5.4b; parameters: --outFilterMultiMapNmax 1, --outFilterMisMatchNmax 2). Bedgraph files of 5’ coverage were generated using bedtools genomecov (Quinlan & Hall, 2010) as described in the TSSCall manual. TSSs were then called with TSSCall (Henriques et al., 2017; https://github.com/NIEHS/TSScall)
Genome_build: mm10
Supplementary_files_format_and_content: bedgraph files contain strand specific 5’ end coverage of reads. Bed files contain output of TSSCall (called transcription start sites).

source_name: mouse cortical neurons

extract_protocol:
Total RNA was extracted as described previously and depleted from rRNA and SRP-RNA
The 5' ExoSeq library construction was performed as described (Afik et al., 2017), with the following alterations. Instead of poly(A)-selected RNA we started with a rRNA and signal recognition particle (SRP)-RNA depleted RNA sample. rRNA & SRP-RNA was depleted using a commercially available kit (RiboCop rRNA depletion kit V1.2 (human/mouse/rat), Lexogen) according to the manufacturer’s instructions with some modifications. For one 5’ ExoSeq library sample, RNA from three depletion reactions (3x 500 ng total RNA) was pooled before library construction.

value: cortical neurons

value: untreated


title: ExoSeq_KCl_Rep1

value: KCl stimulated (30 min)


title: Sample50

type: RNA

city: Toronto

first: Alexander

middle: James

last: Murison

state: Ontario

address: 101 College Street, TMDT room 11-706

country: Canada

institute: University Health Network

description: Total RNA was extracted and analyzed using the nanostring platform

data_processing:
The output of the assay was analyzed by nSolver 4.0 where the mean of the negative spike-in control was used as the threshold of microRNA detection. With the expression profile table generated, patients were then stratified by miR-130a expression using a median split and a Kaplan-Meier curve was drawn by GraphPad Prism 7.
All counted reads are provided in the count matrix. Data normalization was conducted within the Ncounter software that used a median cut-off of the negative detection probes

organism: Homo sapiens

source_name: CBF-AML

growth_protocol: Whole blood extraction from patients

extract_protocol: RNA was extracted from peripheral blood cells (1x10^6-1x10^7) using the RNeasy Mini kit (Qiagen). Briefly, samples were lysed, and total RNA was collected by column extraction according to the manufacturer’s instructions. Samples were then examined by Nanostring, a probe-based assay that detects 827 common miRNAs (https://www.nanostring.com/products/ncounter-assays-panels/immunology/mirna/).

treatment_protocol: All patients received standard 7+3 induction therapy

tag: tissue

value: Peripheral blood

tag: diagnosis

value: CBF-AML

tag: age

tag: Sex

value: F

tag: status after 5 years

value: Alive


title: Sample51

value: M


title: GRO_TTX_CTR_REP5

description:
nascent RNA
GROSEQ


title: Sample44


title: Sample46


title: Sample45


title: Sample47


title: Sample41

value: Deceased


title: Sample48


title: Sample43


title: Sample36


title: Sample35


title: Sample38


title: Sample39


title: Sample32


title: Sample34


title: Sample33


title: ExoSeq_KCl_Rep2


title: Sample30


title: Sample28


title: Sample31


title: Sample22


title: Sample24


title: Sample25


title: Sample49


title: Sample23


title: Sample21


title: Sample40


title: Sample20


title: Sample17


title: Sample18


title: Sample19


title: Sample15


title: Sample14


title: Sample16


title: Sample12


title: Sample13


title: Sample06


title: Sample27


title: Sample11


title: Sample10


title: Sample07


title: Sample09


title: Sample03


title: STAPseq_input_TATA_CCAATbox_library

city: Vienna

first: Vanja

last: Haberle

address: Campus-Vienna-Biocenter 1

country: Austria

institute: The Research Institute of Molecular Pathology (IMP)

description: PCR amplified plasmid DNA (input library)

data_processing:
Library strategy: STAP-seq
Basecalls where performed using CASAVA 1.9.1
Read alignment: 10nt long unique molecular barcode identifier (UMI) was removed from the 5' end of the first read and kept track of for later quantification. Paired-end reads were mapped to a reference containing 250bp long barcoded sequences of all 2,000 promoter candidates and to M. musculus (mm9) genome (for spike-in controls) using Bowtie version 1.2.2 allowing 1 mis-match. Only unique mappings with reverse reads mapping to the end of the candidate sequence were kept, ensuring they correspond to reporter transcripts transcribed from that particular cloned candidate. For paired-end reads that mapped to the same positions, we collapsed those that have identical UMIs as well as those for which the UMIs differed by 1 nucleotide to ensure the counting of unique reporter transcripts.
Quantification and normalization: Tag counts at each position represent the sum of the 5′ ends of UMI collapsed fragments and report the number of unique mRNA molecules initiated at that position. For each dataset, the tag count at every position in each candidate sequence was quantified. We did the same for the 9 spike-in M. musculus promoters, and the counts were used to calculate normalization factors between the datasets. For each spike-in promoter we first assigned factor 1 to the dataset with the lowest count and then calculated down-scaling factors for all other datasets based on their counts for that promoter. Final normalization factors were calculated as median of factors for individual spike-in promoters and were used to normalize raw tag counts for each sample.
Genome_build: hg19
Supplementary_files_format_and_content: Tab-delimited text files include information on the candidate sequences (origin, genotype and sequence) and their counts in the cloned input STAP-seq library. For each dataset a file with raw counts per position in each candidate sequence is provided.

source_name: HCT116 cells

extract_protocol: The TATA-box + CCAAT-box promoter candidate library was cloned from a pool of 2,000 synthesized 300-mer oligonucleotides (Twist Biosciences Inc) into a human STAP-seq screening vector (Addgene #). The candidate sequences spanned 240bp in total, with 205bp upstream and 35bp downstream of the selected TSSs, followed by a 10bp random barcode, and flanked by the Illumina i5 (25bp) and i7 (25bp) adapter sequences upstream and downstream, respectively, serving as constant linkers for amplification and cloning. To comprehensively amplify the oligonucleotide pool (diluted to 1ng/µl) and thereby create the candidate library insert we performed 20 PCR reactions (98°C for 45 seconds (s); followed by 15 cycles of 98°C for 15s, 65°C for 30s, 72°C for 10s) with 1µl diluted oligonucleotide pool as template, using KAPA Hifi Hot Start Ready Mix (KAPA Biosystems; cat. no. KK2602) and primers (fw: TAGAGCATGCACCGGACACTCTTTCCCTACACGACGCTCTTCCGATCT and rev: GGCCGAATTCGTCGAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT) that add extra 23bp to each of the adapters, serving as homology arms for directional cloning of the candidate library insert using In-Fusion HD (Clontech; cat. no. 639650). All PCR reactions were pooled and purified with Agencourt AMPureXP DNA beads (ratio beads/PCR 1.4; cat. no. A63881). Directional cloning of the library insert (amplified oligonucleotide pool) into the STAP-seq screening vector was performed using In-Fusion HD (Clontech; cat. no. 639650) recombination.

treatment_protocol: 4x10^7 BRD4-AID tagged cells per replicate were electroporated with a STAP-seq library and treated with either water (mock) or 500µM auxin (IAA) for 6h.

tag: sample type

value: input


title: STAPseq_BRD4_psAuxin_rep1

description: PCR amplified cDNA (STAP-seq transcript)

tag: cell line

value: CRISPR/Cas9 edited cells

value: auxin (IAA)


title: STAPseq_BRD4_msAuxin_rep2

tag: cell type

value: water (mock)


title: Sample01


title: Sample05


title: STAPseq_BRD4_msAuxin_rep1


title: Sample26


title: Sample02


title: MLL1_psAuxin_BAC_rep1

description:
PCR amplified cDNA (STARR-seq reporter transcript)
MLL1_psAuxin_BAC.bw

data_processing:
Library strategy: STARR-seq
Read alignment:  Paired-end STARR-seq reads were trimmed to 36 bp and mapped as paired-end to a reference hg19 genome and to 9 custom spike-in sequences using Bowtie version 0.12.9 allowing 2 mis-matches. Only uniquely mapping read pairs were retained.
Genomic coverage:  Genomic fragments defined by sequenced read pairs from all replicates of each COF/condition were merged and used to generate coverage at 1bp resolution with GenomicRanges v1.34.0 package in R. Coverage was normalized to tags per million and scaled by a factor obtained from relative spike-in abundance. Coverage was exported into bigWig format with rtracklayer v1.42.2.
Genome_build: hg19
Supplementary_files_format_and_content: BigWig files contain normalized coverage of STARR-seq fragments for merged replicates per COF and condition. Note that the signal is restricted to discrete genomic regions covered by the BAC library.

growth_protocol: Cells were grown in square plates (Thermo Scientific #166508) with a seeding density ~20Mill. cells 2 days/square plate before transfection. For genome-wide STARR-seq library 4x10^8 cells and for BAC library 4x10^7 cells were transfected per condition and replicate.

extract_protocol:
After 6h of treatment cells were harvested and total RNA was isolated using RNeasy Maxi kit (Qiagen #75162) containing beta-Mercaptoethanol supplemented RLT buffer. Spike-in control was added in a 1:1000 ratio to the isolated total RNA. polyA+ mRNA was isolated via Oligo-dT25 beads  (Invitrogen #61005) followed by 1h 37°C TurboDNase I treatment (Invitrogen #AM2238).
Isolated mRNA was cleaned via AMPure XP beads (Beckman Coulter #A63882) 1:1.8 ratio (RNA:beads) followed by reverse transcription utilizing SuperScript III (Invitrogen #18080093) using a gene specific primer (GSP): 50°C for 1h, 70°C for 15min, 4°C for 10min. cDNA was treated with RNaseA (Thermo Fisher #EN0531), 37°C for 1h followed by cleanup via AMPure XP beads 1:1.8 ratio. Next, junction PCR which allows enrichment of reporter transcripts was performed using KAPA 2x HiFi (KapaBiosystems #KK2601) and “junction primers” spanning over the spliced intron utilizing the thermocycler program: 98°C - 45 sec, 98°C - 15 sec, 65°C - 30 sec 16 cycles, 72°C - 70 sec, 72°C - 120 sec followed by purification with AMPure XP 1:0.8 ratio (DNA:beads). Sequencing-ready PCR was performed on the junction PCR products using Illumina primers with the thermocycler program: 98°C - 45 sec, 98°C - 15 sec, 65°C - 30 sec 5 cycles, 72°C - 45 sec, 72°C - 120 sec. Illumina adapter containing STARR-seq library fragments were cleaned using SPRIselect beads (Beckman Coulter #B23318) with a stringent ratio of 1:0.5 (DNA:beads) and subjected to paired end deep sequencing.

treatment_protocol: Genome wide STARR-seq library (Addgene #99296) or BAC STARR-seq library () was electroporated via Maxcyte STX into 85% confluent OsTir1+/-;COF-AID+/+ tagged cells.  After 30min recovery phase cells were split in 2 conditions, receiving medium containing water (mock) or auxin (IAA; 500µM final conc.) for 6h.


title: MLL1_psAuxin_BAC_rep2


title: MLL1_msAuxin_BAC_rep1

description:
PCR amplified cDNA (STARR-seq reporter transcript)
MLL1_msAuxin_BAC.bw


title: Brd9_msAuxin_BAC_rep1

description:
PCR amplified cDNA (STARR-seq reporter transcript)
BRD9_msAuxin_BAC.bw


title: Sample08


title: Brd9_psAuxin_BAC_rep1

description:
PCR amplified cDNA (STARR-seq reporter transcript)
BRD9_psAuxin_BAC.bw


title: Brd7_msAuxin_BAC_rep1

description:
PCR amplified cDNA (STARR-seq reporter transcript)
BRD7_msAuxin_BAC.bw


title: Brd7_msAuxin_BAC_rep2


title: MLL1_msAuxin_BAC_rep2


title: Sample04


title: WT_3hrNutlin_rep1

description:
WT_3hrNutlin.ps.bw
WT_3hrNutlin.ns.bw
WT_3hrNutlin_raw_counts_per_condition_for_promoter_and_genebody_regions.txt

data_processing:
Library strategy: PRO-seq
UMI removal and adapter trimming: Sequenced reads consist of a 10nt long UMI (for counting unique RNA molecules), followed by PolII active site-associated RNA sequence and potentially a part of the Illumina sequencing adapter (in cases where RNA was shorter than 22nt). Prior to mapping, the UMI sequence (10nt at the 5' end of the read) was removed and noted for later counting. Illumina adapter was trimmed off with cutadapt v1.18.
Read alignment: All reads longer than 15nt after adapter trimming were mapped to a merged reference consisting of hg19 and dm3 (spike-in) genome using Bowtie version 0.12.9 allowing 2 mis-matches. Reads mapping to multiple positions were randomly assigned to one mapping position.
Genomic coverage:  5' ends of mapped reads (the last nucleotide transcribed by PolII) were counted per genomic position taking into account only unique UMIs. Coverage was generated separately for positive and negative strands. Coverage was normalized to tags per million and scaled by a factor obtained from relative spike-in abundance. Coverage was generated with GenomicRanges v1.34.0 package in R and exported into bigWig format with rtracklayer v1.42.2.
Quantification and differential analysis:  5' ends of mapped, UMI-collapsed reads overlapping promoter+pause region (from +1 to +150bp downstream of the TSS) and gene body region (from +150bp to annotated gene end) were counted for each individual sample/replicate. Gene annotation from Ensembl v83 was used, and annotated TSSs were corrected to nearest CAGE-defined TSS from FANTOM5 collection. Provided raw counts were used for downstream differential analysis between different conditions with DEseq2 v1.22.2.
Genome_build: hg19
Supplementary_files_format_and_content: Tab-delimited text files include genomic coordinates of a referent set of 21115 genes/transcripts with Ensembl v83 gene/transcript IDs, gene names, TSS positions corrected by CAGE and annotated transcription end sites. For each gene raw counts in each individual replicate are provided, separately for promoter+pause region (from +1 to +150bp downstream of the TSS) and gene body region (from +150bp to annotated gene end).  BigWig files contain spike-in normalized genome-wide coverage of PRO-seq reads, separately for positive and negative strands (the values for negative strand are shown as negative).

molecule: nuclear RNA

extract_protocol:
After treatment the cells were harvested and nuclei were isolated. Spike-in control (D. melanogaster S2 cell nuclei; 1% of total human HCT116 cells/nuclei) were added at the level of nuclei permeabilization step. Nuclear-run-on was performed for 3min at 37°C with biotin labeled CTPs (Perkin Elmer #NEL542001EA) followed by RNA extraction and base hydrolysis. Biotin-tagged nuclear-run-on RNA was enriched via M280 streptavidin beads (Invitrogen #112.06D) and precipitated via Phenol-Chloroform treatment.
3’ RNA adapters were ligated to isolated RNA and second biotin RNA enrichment followed by RNA 5’ cap modification via TAP (Biozym #187005) treatment was performed. Furthermore, 5’ hydroxyl repair via PNK (NEB #M0201S) treatment and subsequent 5’ adapter ligation was carried out. Afterwards, cDNA was generated from enriched RNA via reverse transcription (Super Script III Reverse Transcriptase, Invitrogen #18080-044). 10µl of the cDNA library was amplified via KAPA Amplification reaction (Roche #7959028001) on a qPCR machine (Biorad CFX Connect RealTime System). KAPA reaction: 10µl cDNA, 1µl forward primer 35µM (RP1-RP20), 1µl of reverse primer 35µM (RP1: 5’-AATGATACGGCGACCACCGAGATCTACAGTTCAGAGTTCTACAGTCCGA-3’), 25µl 2x KAPA SYBER master mix, 13µl water. PCR program: 98°C - 45 sec, 98°C - 15 sec, 60°C - 30 sec, 72°C - 30 sec, 72°C - 10 sec. Samples were removed from the qPCR machine after 12-15 cycles and cleaned with Ampure beads (Beckman #A63881) in a 1:1.4 (DNA:beads) ratio. DNA bound to the beads was eluted in 11µl of water and subjected to single-end deep sequencing.

treatment_protocol: 1x10^7 WT or COF-AID tagged cells per replicate were treated as follows:   1. Control:  water (mock) treatment for 3h [MED14- and BRD4-AID-tagged cells] or 12h [MED14-AID-tagged cells], or DMSO treatment for 3h [WT]  2. Nutlin:  10µM Nutlin 3a (Sigma# SML0580) treatment for 3h [WT, MED14- and BRD4-AID-tagged cells] or 6h [MED14-AID-tagged cells]  3. Auxin:  500µM auxin (IAA) treatment for 3h [MED14- and BRD4-AID-tagged cells] or 12h [MED14-AID-tagged cells]  4. Auxin + Nutlin: 500µM auxin (IAA) treatment for 3h and subsequent 10µM Nutlin 3a treatment for 3h [MED14- and BRD4-AID-tagged cells] or 500µM auxin (IAA) treatment for 12h and subsequent 10µM Nutlin 3a treatment for 6h [MED14-AID-tagged cells]

value: wild-type


title: Brd9_psAuxin_BAC_rep2


title: WT_DMSO_rep1

description:
WT_DMSO.ps.bw
WT_DMSO.ns.bw
WT_3hrNutlin_raw_counts_per_condition_for_promoter_and_genebody_regions.txt

value: DMSO (mock)


title: p300CBP_psAuxin_rep4 [STARR-seq]

description:
PCR amplified cDNA (STARR-seq reporter transcript)
p300CBP_psAuxin.bw
Raw_counts_per_COF_and_replicate_for_common_set_of_enhancers.txt
Log2FC_psAuxin_vs_msAuxin_per_COF_for_common_set_of_enhancers.txt

data_processing:
Library strategy: STARR-seq
Read alignment:  Paired-end STARR-seq reads were trimmed to 50 bp and mapped as paired-end to a reference hg19 genome and to 9 custom spike-in sequences using Bowtie version 0.12.9 allowing 3 mis-matches. Only uniquely mapping read pairs were retained.
Genomic coverage:  Genomic fragments defined by sequenced read pairs from all replicates of each COF/condition were merged and used to generate coverage at 1bp resolution with GenomicRanges v1.34.0 package in R. Coverage was normalized to tags per million and scaled by a factor obtained from relative spike-in abundance. Coverage was exported into bigWig format with rtracklayer v1.42.2.
Peak calling:  Unique fragments from merged replicates of each COF/treatment were used to call peaks with macs2 v2.1.2, against previously sequenced STARR-seq input library (Muerdter et al. Nat Methods 2018) as control. Only peaks with more than 3-fold enrichment over input on both strands and at least 3 tags per million were kept. A common referent set of 6249 STARR-seq enhancers was compiled by combining peaks across all COFs/treatments.
Quantification and differential analysis:  Sequenced fragments overlapping each enhancer were counted for each individual replicate of COF/treatment to obtain raw counts per enhancer. For each COF differential analysis between auxin (IAA) and water (mock) treatment was performed with edgeR v3.24.3 supplying custom scaling factors from relative spike-in abundance, and resulting log2 fold-change values for each enhancer were reported.
Genome_build: hg19
Supplementary_files_format_and_content: Tab-delimited text files include genomic coordinates of a referent set of 6249 STARR-seq enhancers compiled across all STARR-seq samples and tag counts at those enhancers as follows: 1) Raw tag counts in each individual replicate of all COFs and treatments are provided in Raw_counts_per_COF_and_replicate_for_common_set_of_enhancers.txt file and 2) Log2 fold-change between auxin (IAA) and water (mock) treatment calculated from normalized counts (normalized to spike-in) after merging replicates per CoF/treatment are provided in the Log2FC_psAuxin_vs_msAuxin_per_COF_for_common_set_of_enhancers.txt file. BigWig files contain normalized coverage of STARR-seq fragments averaged across biological replicates for each COF/treatment.

growth_protocol: 4x10^8 cells were grown in square plates (Thermo Scientific #166508) with a seeding density ~20Mill. cells 2 days/square plate before transfection.

treatment_protocol: Genome wide STARR-seq library (Addgene #99296) was electroporated via Maxcyte STX into 85% confluent OsTir1+/-;COF-AID+/+ tagged cells.  After 30min recovery phase cells were split in 2 conditions, receiving medium containing water (mock) or auxin (IAA; 500µM final conc.; 2x10^8 cells) for 6h.


title: Brd7_psAuxin_BAC_rep2

description:
PCR amplified cDNA (STARR-seq reporter transcript)
BRD7_psAuxin_BAC.bw


title: WT_3hrNutlin_rep2


title: WT_DMSO_rep2


title: STAPseq_BRD4_psAuxin_rep2


title: Brd4_msAuxin_rep4 [STARR-seq]

description:
PCR amplified cDNA (STARR-seq reporter transcript)
Brd4_msAuxin.bw
Raw_counts_per_COF_and_replicate_for_common_set_of_enhancers.txt
Log2FC_psAuxin_vs_msAuxin_per_COF_for_common_set_of_enhancers.txt


title: p300CBP_msAuxin_rep2 [STARR-seq]

description:
PCR amplified cDNA (STARR-seq reporter transcript)
p300CBP_msAuxin.bw
Raw_counts_per_COF_and_replicate_for_common_set_of_enhancers.txt
Log2FC_psAuxin_vs_msAuxin_per_COF_for_common_set_of_enhancers.txt


title: p300CBP_psAuxin_rep2 [STARR-seq]


title: p300CBP_psAuxin_rep3 [STARR-seq]


title: p300CBP_msAuxin_rep3 [STARR-seq]


title: Brd4_psAuxin_rep3 [STARR-seq]

description:
PCR amplified cDNA (STARR-seq reporter transcript)
Brd4_psAuxin.bw
Raw_counts_per_COF_and_replicate_for_common_set_of_enhancers.txt
Log2FC_psAuxin_vs_msAuxin_per_COF_for_common_set_of_enhancers.txt


title: Brd4_psAuxin_rep4 [STARR-seq]


title: p300CBP_msAuxin_rep1 [STARR-seq]


title: Brd2_psAuxin_rep4 [STARR-seq]

description:
PCR amplified cDNA (STARR-seq reporter transcript)
Brd2_psAuxin.bw
Raw_counts_per_COF_and_replicate_for_common_set_of_enhancers.txt
Log2FC_psAuxin_vs_msAuxin_per_COF_for_common_set_of_enhancers.txt


title: Brd4_msAuxin_rep3 [STARR-seq]


title: Brd9_msAuxin_BAC_rep2


title: Brd2_psAuxin_rep2 [STARR-seq]


title: Brd2_psAuxin_rep3 [STARR-seq]


title: Brd2_msAuxin_rep4 [STARR-seq]

description:
PCR amplified cDNA (STARR-seq reporter transcript)
Brd2_msAuxin.bw
Raw_counts_per_COF_and_replicate_for_common_set_of_enhancers.txt
Log2FC_psAuxin_vs_msAuxin_per_COF_for_common_set_of_enhancers.txt


title: Brd2_psAuxin_rep1 [STARR-seq]


title: Brd2_msAuxin_rep3 [STARR-seq]


title: Brd2_msAuxin_rep1 [STARR-seq]


title: Brd7_psAuxin_BAC_rep1


title: Brd2_msAuxin_rep2 [STARR-seq]


title: Parental_psAuxin_rep2 [STARR-seq]

description:
PCR amplified cDNA (STARR-seq reporter transcript)
Parental_psAuxin.bw
Raw_counts_per_COF_and_replicate_for_common_set_of_enhancers.txt
Log2FC_psAuxin_vs_msAuxin_per_COF_for_common_set_of_enhancers.txt


title: Parental_msAuxin_rep2 [STARR-seq]

description:
PCR amplified cDNA (STARR-seq reporter transcript)
Parental_msAuxin.bw
Raw_counts_per_COF_and_replicate_for_common_set_of_enhancers.txt
Log2FC_psAuxin_vs_msAuxin_per_COF_for_common_set_of_enhancers.txt


title: Parental_msAuxin_rep1 [STARR-seq]


title: Parental_psAuxin_rep1 [STARR-seq]


title: p300CBP_psAuxin_rep1 [STARR-seq]


title: p300CBP_msAuxin_rep4 [STARR-seq]


title: small RNA-seq: NIH/3T3-AtRDR1(+)Dox

city: Beijing

first: Li

last: Ding

state: Beijing

address: No.5, Yiheyuan Road

country: China

institute: Peking University

description: Gel-purified 15-50nt small RNAs

data_processing:
To analyze polyA(+) RNA-seq data, adapters of raw data were trimmed with Trimmomatic software. The quality of these data was evaluated by FastQC software. HISAT2 software with default parameters was used to align sequences to the human genome (hg38, UCSC) and the mouse genome (mm10, UCSC) based on the origin of the cell lines. Output files after sequence alignment were used to obtain gene count matrix with featureCounts software using corresponding UCSC RefSeq as gene annotations. For further analyses, gene counts in each sample were normalized to the total reads of the library to calculate reads per million (RPM).
Perl and python scripts were used to analyze small RNA-seq data. Adapters of sequences were trimmed and sequences with a length of 19-25nt were retained. To build the index of miRNA, we downloaded the mature miRNA sequences from miRBase, 3nt upstream and 3nt downstream of mature miRNA were added to the references. Each sequence was aligned to the reference using Bowtie software using the following parameters: -a -v 1 -3 3.In order to compare the miRNA expression in the line, the count of miRNAs was normalized. In RDR1 expression group, two ways were used to normalize miRNA data. Fly miRNA was added as spike-in in some samples. In non-spike-in method (including A549, HeLa, HepG2, HCT116 and NIH/3T3), the numbers of miRNAs were normalized to the numbers of total reads of the corresponding library to calculate RPM. In spike-in method (including H1299, Jurkat and RPE-1), the number of miRNAs was multiplied by a factor to make the count of fly miRNAs equal in all samples in a cell line. We used the top-10 highest expressed miRNAs as reference, which did not contain identical miRNAs in fly and human/mouse. Each of these 10 miRNAs was used to calculate a factor and the mean of these 10 factors was used as the factor for this sample for normalization. After normalization between samples within cell lines, the normalized reads of the sample with higher miRNA expression level was set at one million. In cell cycle inhibition, AGO2-CLIP and AGO2 knockdown experiments, miRNA expression was normalized with non-spike-in method. Some cell lines of RDR1 expression group were used in this analysis (A549, HeLa, HepG2). In order to be consistent with the previous analysis in RDR1 expression group, the normalized reads of the sample with higher miRNA expression level of RDR1 expression group was set at one million. In the miR-34c transfection assay, miRNA expression was normalized to the expression of endogenous miRNA, which was set at one million.
Genome_build: mm10, hg38
Supplementary_files_format_and_content: miRNA exression matrix

source_name: NIH/3T3-AtRDR1 stable cell line

extract_protocol:
TRIzol reagent (Invitrogen) was used to isolate total RNA according to the manufacturer’s instructions.
RNA-seq libraries preparation using the TruSeq Stranded mRNA Sample Prep Kits (Illumina), VAHTS Stranded mRNA-seq Library Prep Kits (Vazyme) and VAHTS Ribo-off Globin & rRNA Depletion Kits(Vazyme). Small RNA-seq libraries preparation using the NEBNext® Small RNA Library Prep Set for Illumina kits. Single-cell RNA-seq libraries preparation using the Chromium Next GEM Single Cell 3’ Kit v3.1 (10x Genomics).

tag: cell type and treatment

value: NIH/3T3-AtRDR1 with Dox induction for 6 days

value: NIH/3T3-AtRDR1 stable cell line


title: WL24_H1993_Inhibition_rep3

city: Vancouver

first: William

last: Lockwood

state: British Columbia

address: 675 West 10th Avenue

department: Integrative Oncology

institute: BC Cancer

hyb_protocol: cDNA was hybridized according to manufacturer's instructions at The Centre for Applied Genomics

scan_protocol: GeneChips were scanned according to manufacturer's instructions at The Centre for Applied Genomics

data_processing: Expression data was RMA normalized using R/Bioconductor

label: biotin

source_name: H1993, 10μM Cabozantinib + 25ng/mL HGF

label_protocol: 150ng of RNA was converted to cDNA according to manufacturer's instructions and labelled at The Centre for Applied Genomics

growth_protocol: All cells were cultured at 37℃ with 5% CO2 in humidified atmosphere. H1993 and Hs746T cells were grown in high glucose DMEM with 10% FBS and 1% Glutamax. H596 cells were grown in RPMI-1640 with with 10% FBS and 1% Glutamax.

extract_protocol: Total RNA was extracted from biological triplicates using a Zymo Quick-RNA Miniprep Kit. Sample quality was assessed using an Agilent Bioanalyzer and subsequent sample preparation, array hybridization, and data acquisition was performed by the Centre for Applied Genomics Microarray Facility (Toronto, Ontario).

treatment_protocol: Cells were treated with: 1) 0.1% DMSO, 2) 0.1% DMSO + 25ng/mL HGF, or 3) 10μM Cabozantinib + 25ng/mL HGF for 6 hours

value: 10uM Cabozantinib + 25ng/mL HGF


title: WL25_H596_Inhibition_rep1

source_name: H596, 10μM Cabozantinib + 25ng/mL HGF


title: WL19_Hs746T_Inhibition_rep1

source_name: Hs746T, 10μM Cabozantinib + 25ng/mL HGF


title: WL23_H1993_Inhibition_rep2


title: WL22_H1993_Inhibition_rep1


title: WL21_Hs746T_Inhibition_rep3


title: WL20_Hs746T_Inhibition_rep2


title: WL27_H596_Inhibition_rep3


title: WL16_H596_Stimulation_rep1

source_name: H596, 0.1% DMSO + 25ng/mL HGF

value: 0.1% DMSO + 25ng/mL HGF


title: WL12_Hs746T_Stimulation_rep3

source_name: Hs746T, 0.1% DMSO + 25ng/mL HGF


title: WL14_H1993_Stimulation_rep2


title: WL15_H1993_Stimulation_rep3


title: WL17_H596_Stimulation_rep2


title: WL26_H596_Inhibition_rep2


title: WL05_H1993_Untreated_rep2

value: 0.1% DMSO


title: WL11_Hs746T_Stimulation_rep2


title: WL08_H596_Untreated_rep2


title: KO2 spermatocytes

city: Chaoyang district

first: Yanze

last: Song

address: Datun Road

department: Regenerative Medicine

institute: The Chinese Academay of Sciences

data_processing:
Clean data of RNA sequencing reads were aligned to mouse reference sequence GRCm39 using STAR (2.7.0f)
Read counts and TPM (Transcripts Per Kilobase Million) were counted using RSEM (1.3.2).
Differential expression genes (DEG) (FDR < 0.05, log2 (fold change) (log2 FC) ≥ 1 or ≤ −1) were calculated by edgeR (3.28.1) package. The DEG genes were carried out Gene Ontology Pathway analysis and Kyoto Encyclopedia of Genes and Genomes analysis by clusterProfiler (3.14.3) and org.Hs.eg.db (3.10.0) packages.
Heatmap (1.0.12) was used for the heatmap visualization; colors represent the Z-score derived from the log2 transformed FPKM data. Principal Component Analysis (PCA) was performed by R packages, including tidyr(1.1.2), dplyr (1.0.2) and ggplot2 (3.3.2).
Genome_build: Genome_build: mm9
Supplementary_files_format_and_content: expected_count, TPM, FPKM

source_name: Primary spermatocyte

extract_protocol:
Primary spermatocytes were collected using flow cytometry via Hoechst staining of single cell suspension of testes and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 2 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols

tag: genotype/variation

value: circSry KO

value: spermatocyte

value: 8 week-old


title: small RNA-seq: NIH/3T3-WT

description: Gel-purified 15-50nt small RNAs

value: WT

tag: genotype


title: WL02_Hs746T_Untreated_rep2


title: WL09_H596_Untreated_rep3


title: WL01_Hs746T_Untreated_rep1


title: WL07_H596_Untreated_rep1


title: WT2 spermatocytes

value: wild type


title: WL06_H1993_Untreated_rep3


title: WL04_H1993_Untreated_rep1


title: WL03_Hs746T_Untreated_rep3


title: WL18_H596_Stimulation_rep3


title: CM2019

description:
barcode region of lentiviral backbones from genomic DNA from bulk tumor-bearing lungs were PCR-amplified and high-throughput DNA sequenced
oncogene_negative_LUAD_tumor_sizes.csv

tag: experiment

value: KT

tag: drug treatment

value: Untreated

tag: molecule subtype

value: genomic barcoded DNA


title: MY1678


title: MT406


title: MT350


title: WL13_H1993_Stimulation_rep1

tag: treatment


title: MT291


title: MY1863

value: Pten;TC


title: KO1 spermatocytes

tag: strain

tag: developmental stage


title: CM2240


title: CM2033


title: WT3 spermatocytes


title: MY1676


title: WL10_Hs746T_Stimulation_rep1


title: MT404


title: CM2032


title: MT293


title: MT448

value: TC


title: MT415


title: MY1745


title: MY1683


title: MY1874


title: MY1876


title: MY1868


title: MY1881


title: MT453


title: MY1869


title: MT450


title: MY1864


title: CM2245


title: MY1750


title: CM2241


title: MY1746


title: MT408


title: MT349


title: MT409


title: MY1675


title: MT449


title: MT451


title: MT414


title: MY1870


title: CM2006


title: MT349-Liver


title: MT273


title: MY1748


title: MY1686


title: MY1865


title: MT289


title: MT405


title: CM2176


title: MT446


title: CM2174


title: MY1878


title: CM2017


title: MY1747


title: MT413


title: MY1749


title: MY1867


title: MY1875


title: WL697

description:
barcode region of lentiviral backbones from genomic DNA from one lung lobe per mouse were PCR-amplified and high-throughput DNA sequenced
oncogene_negative_LUAD_tumor_sizes.csv

source_name: one lung lobe


title: MY1685


title: ZR1843


title: MY1687


title: MT272


title: MY1752


title: MY1877


title: MT445


title: RT1860


title: MY1685-Liver


title: RT1853


title: MT444


title: MT410


title: WL691


title: HC054


title: WL436


title: HC005


title: WL684


title: ZR1842


title: WL527


title: RT1866


title: WL521


title: RT1859


title: RT1862


title: WL438


title: WL 195


title: WL697-T8

description:
barcode region of lentiviral backbones from genomic DNA of dissected tumors were PCR-amplified and high-throughput DNA sequenced
oncogene_negative_LUAD_tumor_sizes.csv

source_name: dissected lung tumors


title: WL697-T7


title: HC030


title: HC008


title: WL526


title: MY1880


title: HC048


title: HC002


title: WL697-T3


title: WL 510


title: WL697-T1


title: WL 199


title: WL 198


title: WL691-T2


title: WL697-T6-n


title: WL691-T1


title: WL684-T5


title: WL684-T2


title: HC013


title: WL697-T5-9


title: WL697-T4


title: WL697-T2


title: WL691-T3,T4


title: WL684-T8


title: WL684-T7


title: WL510 (pooled dissected tumors)


title: WL684-T1


title: WL684-T3


title: WL527 (pooled dissected tumors)


title: WL199(pooled dissected tumors)


title: WL526 (pooled dissected tumors)


title: WL513-DT


title: RT1862-T3


title: WL195 (pooled dissected tumors)


title: RT1860-T1-n


title: ZR1859-T1


title: RT1862-T5-n


title: RT1862-Thymus


title: HC013-Thymus


title: RT1862-T1


title: RT1862-T4


title: RT1862-T2


title: HC013-T2,3


title: HC013-Liver


title: WL438-T1


title: WL436-T2,3


title: WL438-Liver


title: HC013-T1


title: HC048-Thymus


title: HC048-T2


title: HC048-Liver


title: HC002-T2,3


title: HC048-T1


title: WL436-T1


title: HC005-Spleen


title: HC002-T1


title: ChIP_NELFA_Un

description: CHIPSEQ

value: Cortical neurons

value: TTX

tag: antibody

value: NELF-A (Santa Cruz Biotech; sc-23599)


title: ChIP_NELFA_KCl_30min

description: CHIPSEQ

value: TTX/ KCl


title: KIR7_S98

first: Mark

last: Davis

address: Stanford University

institute: Institute for Immunity, Transplantation and Infection

data_processing:
FASTQ files for each cell were extracted and generated, distinguished by the unique dual index adaptors.
Reads were aligned to the GRCh38 genome using STAR v2.6.1d.
Transcript abundance was quantified using HTSeq v0.5.4p5.
Genome_build: hg38
Supplementary_files_format_and_content: csv file including transcript abundance quantified by HTSeq for each cell
Supplementary_files_format_and_content: metadata for each cell
Supplementary_files_format_and_content: metadata with TCR information for each cell

source_name: PBMC

extract_protocol:
Blood KIR+ CD8+ T cells (Live CD3+CD56–CD8+TCRαβ+KIR+ cells) were sorted into 96-well plates and cDNA synthesis was performed using the Smart-seq2 protocol
Illumina sequencing libraries were prepared using a Mosquito HTS liquid handler (TTP Labtech). In brief, tagmentation was carried out on 0.4 μl double-stranded cDNA using the Nextera XT DNA Library Preparation Kit (Illumina, FC-131-1096). Each well was mixed with 0.8 μl Nextera tagmentation DNA buffer (Illumina) and 0.4 μl Amplicon Tagment Mix (Illumina), then incubated at 55°C for 10 min. The reaction was stopped by adding 0.4 μl Neutralize Tagment Buffer (Illumina) and centrifuging at room temperature at 3000 g for 5 min. Indexing PCR reactions were performed by adding 0.8 μl of pre-mixed 5 μM i5 and i7 unique dual indexing primers (IDT, customized) and 1.2 μL of Nextera NPM mix (Illumina). PCR amplification was carried out on a C1000 Touch™ Thermal Cycler with 384-Well Reaction Module (Bio-rad) using the following program: 1) 72°C for 3 min, 2) 95°C for 30 s, 3) 12 cycles of 95°C for 10 s, 55°C for 30 s and 72°C for 1 min, and 4) 72°C for 5 min.

tag: tissue

value: PBMC

value: KIR+ CD8 T


title: KIR7_S97


title: Input_NELFA

description: CHIPSEQ


title: KIR7_S92


title: KIR7_S89


title: KIR7_S99


title: KIR7_S94


title: KIR7_S9


title: KIR7_S93


title: HC048-T3,4


title: KIR7_S91


title: KIR7_S87


title: KIR7_S86


title: KIR7_S85


title: KIR7_S90


title: KIR7_S83


title: KIR7_S81


title: HC030-T1


title: KIR7_S80


title: KIR7_S88


title: KIR7_S8


title: KIR7_S82


title: HC005-T1


title: KIR7_S768


title: KIR7_S79


title: KIR7_S77


title: KIR7_S767


title: KIR7_S765


title: KIR7_S76


title: KIR7_S75


title: KIR7_S748


title: KIR7_S751


title: KIR7_S750


title: KIR7_S747


title: KIR7_S743


title: KIR7_S84


title: KIR7_S741


title: KIR7_S746


title: KIR7_S745


title: KIR7_S740


title: KIR7_S734


title: KIR7_S732


title: KIR7_S729


title: KIR7_S728


title: KIR7_S730


title: KIR7_S723


title: KIR7_S724


title: KIR7_S731


title: KIR7_S726


title: KIR7_S721


title: KIR7_S720


title: KIR7_S719


title: KIR7_S72


title: KIR7_S718


title: KIR7_S716


title: KIR7_S722


title: KIR7_S714


title: KIR7_S715


title: KIR7_S717


title: KIR7_S71


title: KIR7_S709


title: KIR7_S735


title: KIR7_S713


title: KIR7_S733


title: KIR7_S710


title: KIR7_S706


title: KIR7_S708


title: KIR7_S707


title: KIR7_S705


title: KIR7_S704


title: KIR7_S727


title: KIR7_S725


title: KIR7_S700


title: KIR7_S703


title: KIR7_S7


title: KIR7_S702


title: KIR7_S701


title: KIR7_S699


title: KIR7_S696


title: KIR7_S693


title: KIR7_S712


title: KIR7_S698


title: KIR7_S697


title: KIR7_S694


title: KIR7_S695


title: KIR7_S692


title: KIR7_S685


title: KIR7_S686


title: KIR7_S687


title: KIR7_S682


title: KIR7_S681


title: KIR7_S69


title: KIR7_S680


title: KIR7_S690


title: KIR7_S691


title: KIR7_S684


title: KIR7_S688


title: KIR7_S68


title: KIR7_S678


title: KIR7_S70


title: KIR7_S677


title: KIR7_S679


title: KIR7_S675


title: KIR7_S671


title: KIR7_S673


title: KIR7_S674


title: KIR7_S67


title: KIR7_S670


title: KIR7_S668


title: KIR7_S683


title: KIR7_S667


title: KIR7_S689


title: KIR7_S666


title: KIR7_S665


title: KIR7_S664


title: KIR7_S663


title: KIR7_S661


title: KIR7_S662


title: KIR7_S66


title: KIR7_S655


title: KIR7_S656


title: KIR7_S654


title: KIR7_S658


title: KIR7_S676


title: KIR7_S649


title: KIR7_S651


title: KIR7_S65


title: KIR7_S657


title: KIR7_S648


title: KIR7_S653


title: KIR7_S646


title: KIR7_S645


title: KIR7_S643


title: KIR7_S642


title: KIR7_S641


title: KIR7_S64


title: KIR7_S660


title: KIR7_S637


title: KIR7_S639


title: KIR7_S638


title: KIR7_S636


title: KIR7_S635


title: KIR7_S634


title: KIR7_S672


title: KIR7_S63


title: KIR7_S629


title: KIR7_S630


title: KIR7_S633


title: KIR7_S632


title: KIR7_S626


title: KIR7_S627


title: KIR7_S624


title: KIR7_S625


title: KIR7_S644


title: KIR7_S622


title: KIR7_S640


title: KIR7_S621


title: KIR7_S620


title: KIR7_S62


title: KIR7_S619


title: KIR7_S623


title: KIR7_S616


title: KIR7_S615


title: KIR7_S618


title: KIR7_S612


title: KIR7_S617


title: KIR7_S613


title: KIR7_S61


title: KIR7_S614


title: KIR7_S631


title: KIR7_S610


title: KIR7_S628


title: KIR7_S608


title: KIR7_S609


title: KIR7_S607


title: KIR7_S605


title: KIR7_S601


title: KIR7_S603


title: KIR7_S602


title: KIR7_S600


title: KIR7_S6


title: KIR7_S60


title: KIR7_S58


title: KIR7_S59


title: KIR7_S57


title: KIR7_S598


title: KIR7_S596


title: KIR7_S599


title: KIR7_S611


title: KIR7_S56


title: KIR7_S545


title: KIR7_S546


title: KIR7_S55


title: KIR7_S548


title: KIR7_S547


title: KIR7_S544


title: KIR7_S541


title: KIR7_S542


title: KIR7_S606


title: KIR7_S543


title: KIR7_S604


title: KIR7_S540


title: KIR7_S54


title: KIR7_S538


title: KIR7_S539


title: KIR7_S535


title: KIR7_S536


title: KIR7_S537


title: KIR7_S534


title: KIR7_S597


title: KIR7_S533


title: KIR7_S532


title: KIR7_S531


title: KIR7_S53


title: KIR7_S530


title: KIR7_S527


title: KIR7_S529


title: KIR7_S526


title: KIR7_S523


title: KIR7_S525


title: KIR7_S524


title: KIR7_S522


title: KIR7_S521


title: KIR7_S520


title: KIR7_S518


title: KIR7_S519


title: KIR7_S516


title: KIR7_S513


title: KIR7_S515


title: KIR7_S517


title: KIR7_S511


title: KIR7_S512


title: KIR7_S51


title: KIR7_S509


title: KIR7_S508


title: KIR7_S506


title: KIR7_S505


title: KIR7_S504


title: KIR7_S528


title: KIR7_S50


title: KIR7_S5


title: KIR7_S502


title: KIR7_S49


title: KIR7_S46


title: KIR7_S48


title: KIR7_S52


title: KIR7_S453


title: KIR7_S454


title: KIR7_S514


title: KIR7_S451


title: KIR7_S452


title: KIR7_S448


title: KIR7_S450


title: KIR7_S45


title: KIR7_S449


title: KIR7_S444


title: KIR7_S447


title: KIR7_S445


title: KIR7_S441


title: KIR7_S443


title: KIR7_S442


title: KIR7_S507


title: KIR7_S44


title: KIR7_S503


title: KIR7_S439


title: KIR7_S438


title: KIR7_S435


title: KIR7_S437


title: KIR7_S434


title: KIR7_S431


title: KIR7_S436


title: KIR7_S430


title: KIR7_S47


title: KIR7_S432


title: KIR7_S425


title: KIR7_S422


title: KIR7_S428


title: KIR7_S429


title: KIR7_S424


title: KIR7_S426


title: KIR7_S421


title: KIR7_S420


title: KIR7_S416


title: KIR7_S418


title: KIR7_S419


title: KIR7_S42


title: KIR7_S417


title: KIR7_S427


title: KIR7_S414


title: KIR7_S411


title: KIR7_S412


title: KIR7_S415


title: KIR7_S409


title: KIR7_S410


title: KIR7_S40


title: KIR7_S41


title: KIR7_S360


title: KIR7_S37


title: KIR7_S4


title: KIR7_S39


title: KIR7_S36


title: KIR7_S356


title: KIR7_S38


title: KIR7_S361


title: KIR7_S359


title: KIR7_S355


title: KIR7_S358


title: KIR7_S352


title: KIR7_S353


title: KIR7_S354


title: KIR7_S351


title: KIR7_S347


title: KIR7_S350


title: KIR7_S357


title: KIR7_S344


title: KIR7_S338


title: KIR7_S35


title: KIR7_S333


title: KIR7_S331


title: KIR7_S337


title: KIR7_S349


title: KIR7_S348


title: KIR7_S346


title: KIR7_S33


title: KIR7_S343


title: KIR7_S345


title: KIR7_S340


title: KIR7_S34


title: KIR7_S329


title: KIR7_S341


title: KIR7_S328


title: KIR7_S339


title: KIR7_S327


title: KIR7_S336


title: KIR7_S322


title: KIR7_S334


title: KIR7_S332


title: KIR7_S330


title: KIR7_S319


title: KIR7_S317


title: KIR7_S316


title: KIR7_S318


title: KIR7_S314


title: KIR7_S335


title: KIR7_S323


title: KIR7_S313


title: KIR7_S325


title: KIR7_S326


title: KIR7_S321


title: KIR7_S320


title: KIR7_S307


title: KIR7_S32


title: KIR7_S31


title: KIR7_S309


title: KIR7_S308


title: KIR7_S324


title: KIR7_S302


title: KIR7_S30


title: KIR7_S301


title: KIR7_S310


title: KIR7_S311


title: KIR7_S306


title: KIR7_S305


title: KIR7_S315


title: KIR7_S297


title: KIR7_S298


title: KIR7_S304


title: KIR7_S3


title: KIR7_S294


title: KIR7_S293


title: KIR7_S292


title: KIR7_S295


title: KIR7_S291


title: KIR7_S300


title: KIR7_S303


title: KIR7_S29


title: KIR7_S285


title: KIR7_S286


title: KIR7_S296


title: KIR7_S287


title: KIR7_S284


title: KIR7_S289


title: KIR7_S288


title: KIR7_S283


title: KIR7_S281


title: KIR7_S299


title: KIR7_S28


title: KIR7_S277


title: KIR7_S279


title: KIR7_S273


title: KIR7_S278


title: KIR7_S27


title: KIR7_S275


title: KIR7_S274


title: KIR7_S280


title: KIR7_S25


title: KIR7_S222


title: KIR7_S219


title: KIR7_S221


title: KIR7_S22


title: KIR7_S271


title: KIR7_S290


title: KIR7_S270


title: KIR7_S223


title: KIR7_S26


title: KIR7_S220


title: KIR7_S282


title: KIR7_S210


title: KIR7_S21


title: KIR7_S23


title: KIR7_S214


title: KIR7_S216


title: KIR7_S215


title: KIR7_S272


title: KIR7_S212


title: KIR7_S211


title: KIR7_S207


title: KIR7_S205


title: KIR7_S206


title: KIR7_S202


title: KIR7_S2


title: KIR7_S217


title: KIR7_S203


title: KIR7_S199


title: KIR7_S198


title: KIR7_S201


title: KIR7_S208


title: KIR7_S195


title: KIR7_S200


title: KIR7_S197


title: KIR7_S196


title: KIR7_S193


title: KIR7_S191


title: KIR7_S190


title: KIR7_S209


title: KIR7_S204


title: KIR7_S188


title: KIR7_S194


title: KIR7_S187


title: KIR7_S186


title: KIR7_S185


title: KIR7_S19


title: KIR7_S184


title: KIR7_S183


title: KIR7_S20


title: KIR7_S181


title: KIR7_S180


title: KIR7_S18


title: KIR7_S179


title: KIR7_S182


title: KIR7_S192


title: KIR7_S176


title: KIR7_S170


title: KIR7_S171


title: KIR7_S177


title: KIR7_S178


title: KIR7_S173


title: KIR7_S17


title: KIR7_S162


title: KIR7_S172


title: KIR7_S169


title: KIR7_S168


title: KIR7_S166


title: KIR7_S160


title: KIR7_S167


title: KIR7_S158


title: KIR7_S165


title: KIR7_S164


title: KIR7_S163


title: KIR7_S218


title: KIR7_S154


title: KIR7_S152


title: KIR7_S175


title: KIR7_S150


title: KIR7_S161


title: KIR7_S156


title: KIR7_S157


title: KIR7_S149


title: KIR7_S153


title: KIR7_S174


title: KIR7_S159


title: KIR7_S148


title: KIR7_S151


title: KIR7_S146


title: KIR7_S144


title: KIR7_S147


title: KIR7_S143


title: KIR7_S141


title: KIR7_S14


title: KIR7_S142


title: KIR7_S140


title: KIR7_S138


title: KIR7_S155


title: KIR7_S139


title: KIR7_S137


title: KIR7_S135


title: KIR7_S136


title: KIR7_S132


title: KIR7_S133


title: KIR7_S13


title: KIR7_S129


title: KIR7_S15


title: KIR7_S127


title: KIR7_S130


title: KIR7_S126


title: KIR7_S125


title: KIR7_S123


title: KIR7_S124


title: KIR7_S122


title: KIR7_S121


title: KIR7_S119


title: KIR7_S116


title: KIR7_S117


title: KIR7_S120


title: KIR7_S115


title: KIR7_S114


title: KIR7_S111


title: KIR7_S113


title: KIR7_S112


title: KIR7_S131


title: KIR7_S134


title: KIR7_S110


title: KIR7_S11


title: KIR7_S108


title: KIR7_S1


title: KIR7_S100


title: KIR7_S106


title: KIR7_S107


title: KIR7_S105


title: KIR7_S128


title: KIR7_S104


title: KIR6_S85


title: KIR7_S103


title: KIR7_S102


title: KIR7_S101


title: KIR6_S83


title: KIR6_S9


title: KIR7_S10


title: KIR6_S87


title: KIR6_S8


title: KIR7_S118


title: KIR6_S86


title: KIR6_S77


title: KIR6_S768


title: KIR6_S80


title: KIR6_S79


title: KIR6_S78


title: KIR6_S765


title: KIR6_S764


title: KIR6_S82


title: KIR6_S766


title: KIR7_S109


title: KIR6_S767


title: KIR6_S763


title: KIR6_S76


title: KIR6_S759


title: KIR6_S761


title: KIR6_S758


title: KIR6_S754


title: KIR6_S757


title: KIR6_S84


title: KIR6_S750


title: KIR6_S752


title: KIR6_S751


title: KIR6_S75


title: KIR6_S749


title: KIR6_S753


title: KIR6_S743


title: KIR6_S741


title: KIR6_S740


title: KIR6_S738


title: KIR6_S736


title: KIR6_S735


title: KIR6_S74


title: KIR6_S733


title: KIR6_S725


title: KIR6_S737


title: KIR6_S721


title: KIR6_S719


title: KIR6_S730


title: KIR6_S729


title: KIR6_S728


title: KIR6_S756


title: KIR6_S746


title: KIR6_S718


title: KIR6_S713


title: KIR6_S727


title: KIR6_S711


title: KIR6_S724


title: KIR6_S723


title: KIR6_S722


title: KIR6_S720


title: KIR6_S72


title: KIR6_S747


title: KIR6_S716


title: KIR6_S710


title: KIR6_S715


title: KIR6_S714


title: KIR6_S709


title: KIR6_S712


title: KIR6_S705


title: KIR6_S70


title: KIR6_S702


title: KIR6_S698


title: KIR6_S71


title: KIR6_S697


title: KIR6_S708


title: KIR6_S734


title: KIR6_S707


title: KIR6_S696


title: KIR6_S695


title: KIR6_S693


title: KIR6_S691


title: KIR6_S703


title: KIR6_S688


title: KIR6_S690


title: KIR6_S699


title: KIR6_S701


title: KIR6_S686


title: KIR6_S7


title: KIR6_S700


title: KIR6_S684


title: KIR6_S704


title: KIR6_S685


title: KIR6_S692


title: KIR6_S706


title: KIR6_S687


title: KIR6_S689


title: KIR6_S683


title: KIR6_S694


title: KIR6_S678


title: KIR6_S67


title: KIR6_S680


title: KIR6_S66


title: KIR6_S64


title: KIR6_S681


title: KIR6_S629


title: KIR6_S68


title: KIR6_S624


title: KIR6_S625


title: KIR6_S63


title: KIR6_S623


title: KIR6_S679


title: KIR6_S65


title: KIR6_S62


title: KIR6_S620


title: KIR6_S619


title: KIR6_S621


title: KIR6_S628


title: KIR6_S627


title: KIR6_S626


title: KIR6_S682


title: KIR6_S622


title: KIR6_S677


title: KIR6_S618


title: KIR6_S616


title: KIR6_S630


title: KIR6_S615


title: KIR6_S613


title: KIR6_S617


title: KIR6_S610


title: KIR6_S614


title: KIR6_S611


title: KIR6_S612


title: KIR6_S607


title: KIR6_S609


title: KIR6_S61


title: KIR6_S608


title: KIR6_S604


title: KIR6_S603


title: KIR6_S602


title: KIR6_S600


title: KIR6_S606


title: KIR6_S594


title: KIR6_S595


title: KIR6_S60


title: KIR6_S605


title: KIR6_S601


title: KIR6_S6


title: KIR6_S596


title: KIR6_S597


title: KIR6_S592


title: KIR6_S599


title: KIR6_S598


title: KIR6_S589


title: KIR6_S588


title: KIR6_S584


title: KIR6_S593


title: KIR6_S583


title: KIR6_S582


title: KIR6_S590


title: KIR6_S591


title: KIR6_S587


title: KIR6_S59


title: KIR6_S581


title: KIR6_S586


title: KIR6_S577


title: KIR6_S58


title: KIR6_S579


title: KIR6_S578


title: KIR6_S576


title: KIR6_S585


title: KIR6_S573


title: KIR6_S580


title: KIR6_S572


title: KIR6_S575


title: KIR6_S571


title: KIR6_S568


title: KIR6_S569


title: KIR6_S562


title: KIR6_S565


title: KIR6_S567


title: KIR6_S564


title: KIR6_S563


title: KIR6_S560


title: KIR6_S561


title: KIR6_S556


title: KIR6_S557


title: KIR6_S554


title: KIR6_S56


title: KIR6_S570


title: KIR6_S552


title: KIR6_S551


title: KIR6_S550


title: KIR6_S559


title: KIR6_S549


title: KIR6_S558


title: KIR6_S548


title: KIR6_S547


title: KIR6_S545


title: KIR6_S555


title: KIR6_S544


title: KIR6_S542


title: KIR6_S541


title: KIR6_S57


title: KIR6_S540


title: KIR6_S566


title: KIR6_S55


title: KIR6_S546


title: KIR6_S5


title: KIR6_S553


title: KIR6_S495


title: KIR6_S493


title: KIR6_S543


title: KIR6_S488


title: KIR6_S54


title: KIR6_S49


title: KIR6_S486


title: KIR6_S487


title: KIR6_S53


title: KIR6_S497


title: KIR6_S496


title: KIR6_S50


title: KIR6_S490


title: KIR6_S482


title: KIR6_S494


title: KIR6_S492


title: KIR6_S51


title: KIR6_S489


title: KIR6_S475


title: KIR6_S474


title: KIR6_S491


title: KIR6_S48


title: KIR6_S481


title: KIR6_S484


title: KIR6_S483


title: KIR6_S476


title: KIR6_S469


title: KIR6_S468


title: KIR6_S478


title: KIR6_S466


title: KIR6_S464


title: KIR6_S477


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title: KIR6_S52


title: KIR6_S463


title: KIR6_S472


title: KIR6_S473


title: KIR6_S471


title: KIR6_S457


title: KIR6_S470


title: KIR6_S47


title: KIR6_S453


title: KIR6_S45


title: KIR6_S450


title: KIR6_S467


title: KIR6_S44


title: KIR6_S41


title: KIR6_S461


title: KIR6_S465


title: KIR6_S462


title: KIR6_S460


title: KIR6_S46


title: KIR6_S4


title: KIR6_S459


title: KIR6_S398


title: KIR6_S456


title: KIR6_S395


title: KIR6_S458


title: KIR6_S394


title: KIR6_S42


title: KIR6_S43


title: KIR6_S451


title: KIR6_S402


title: KIR6_S390


title: KIR6_S40


title: KIR6_S400


title: KIR6_S399


title: KIR6_S397


title: KIR6_S387


title: KIR6_S389


title: KIR6_S452


title: KIR6_S386


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title: KIR6_S385


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title: KIR6_S384


title: KIR6_S383


title: KIR6_S393


title: KIR6_S403


title: KIR6_S391


title: KIR6_S401


title: KIR6_S388


title: KIR6_S378


title: KIR6_S38


title: KIR6_S381


title: KIR6_S39


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title: KIR6_S370


title: KIR6_S371


title: KIR6_S374


title: KIR6_S379


title: KIR6_S377


title: KIR6_S368


title: KIR6_S37


title: KIR6_S366


title: KIR6_S364


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title: KIR6_S372


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title: KIR6_S357


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title: KIR6_S355


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title: KIR6_S13


title: KIR6_S11


title: KIR6_S12


title: KIR6_S10


title: KIR5_S99


title: KIR6_S1


title: KIR5_S98


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title: KIR5_S93


title: KIR5_S89


title: KIR5_S91


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title: KIR6_S135


title: KIR6_S14


title: KIR5_S86


title: KIR5_S8


title: KIR5_S80


title: KIR5_S766


title: KIR5_S82


title: KIR5_S767


title: KIR5_S84


title: KIR5_S768


title: KIR5_S77


title: KIR5_S79


title: KIR5_S763


title: KIR5_S765


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title: KIR5_S761


title: KIR5_S762


title: KIR5_S756


title: KIR5_S758


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title: KIR5_S747


title: KIR5_S745


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title: KIR5_S748


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title: KIR5_S740


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title: KIR5_S732


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title: KIR5_S72


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title: KIR5_S717


title: KIR5_S716


title: KIR5_S736


title: KIR5_S708


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title: KIR5_S706


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title: KIR5_S196


title: KIR5_S193


title: KIR5_S191


title: KIR5_S188


title: KIR5_S19


title: KIR5_S190


title: KIR5_S187


title: KIR5_S186


title: KIR5_S185


title: KIR5_S212


title: KIR5_S18


title: KIR5_S181


title: KIR5_S182


title: KIR5_S184


title: KIR5_S180


title: KIR5_S179


title: KIR5_S177


title: KIR5_S183


title: KIR5_S174


title: KIR5_S176


title: KIR5_S171


title: KIR5_S173


title: KIR5_S172


title: KIR5_S17


title: KIR5_S2


title: KIR5_S170


title: KIR5_S169


title: KIR5_S168


title: KIR5_S167


title: KIR5_S166


title: KIR5_S192


title: KIR5_S163


title: KIR5_S162


title: KIR5_S164


title: KIR5_S161


title: KIR5_S159


title: KIR5_S160


title: KIR5_S16


title: KIR5_S175


title: KIR5_S153


title: KIR5_S157


title: KIR5_S148


title: KIR5_S155


title: KIR5_S145


title: KIR5_S158


title: KIR5_S142


title: KIR5_S143


title: KIR5_S152


title: KIR5_S150


title: KIR5_S14


title: KIR5_S147


title: KIR5_S137


title: KIR5_S139


title: KIR5_S149


title: KIR5_S144


title: KIR5_S138


title: KIR5_S135


title: KIR5_S133


title: KIR5_S136


title: KIR5_S134


title: KIR5_S165


title: KIR5_S131


title: KIR5_S130


title: KIR5_S13


title: KIR5_S127


title: KIR5_S128


title: KIR5_S12


title: KIR5_S141


title: KIR5_S120


title: KIR5_S125


title: KIR5_S119


title: KIR5_S116


title: KIR5_S118


title: KIR5_S121


title: KIR5_S123


title: KIR5_S11


title: KIR5_S112


title: KIR5_S113


title: KIR5_S109


title: KIR5_S110


title: KIR5_S108


title: KIR5_S107


title: KIR5_S105


title: KIR5_S102


title: KIR5_S104


title: KIR5_S103


title: KIR5_S106


title: KIR5_S100


title: KIR5_S1


title: KIR5_S126


title: KIR4_S99


title: KIR5_S124


title: KIR4_S98


title: KIR4_S97


title: KIR4_S91


title: KIR4_S9


title: KIR5_S117


title: KIR5_S111


title: KIR4_S93


title: KIR4_S92


title: KIR4_S87


title: KIR4_S94


title: KIR4_S86


title: KIR5_S122


title: KIR4_S88


title: KIR4_S89


title: KIR4_S83


title: KIR4_S84


title: KIR4_S85


title: KIR4_S81


title: KIR5_S101


title: KIR4_S79


title: KIR4_S78


title: KIR4_S8


title: KIR4_S90


title: KIR4_S82


title: KIR4_S77


title: KIR4_S768


title: KIR4_S80


title: KIR4_S767


title: KIR4_S766


title: KIR4_S765


title: KIR4_S76


title: KIR4_S756


title: KIR4_S754


title: KIR4_S753


title: KIR4_S751


title: KIR4_S75


title: KIR4_S72


title: KIR4_S74


title: KIR4_S70


title: KIR4_S71


title: KIR4_S69


title: KIR4_S7


title: KIR4_S68


title: KIR4_S63


title: KIR4_S64


title: KIR4_S62


title: KIR4_S61


title: KIR4_S66


title: KIR4_S65


title: KIR4_S6


title: KIR4_S56


title: KIR4_S57


title: KIR4_S60


title: KIR4_S55


title: KIR4_S54


title: KIR4_S53


title: KIR4_S52


title: KIR4_S516


title: KIR4_S513


title: KIR4_S755


title: KIR4_S514


title: KIR4_S511


title: KIR4_S512


title: KIR4_S510


title: KIR4_S51


title: KIR4_S509


title: KIR4_S508


title: KIR4_S507


title: KIR4_S506


title: KIR4_S502


title: KIR4_S505


title: KIR4_S503


title: KIR4_S67


title: KIR4_S504


title: KIR4_S58


title: KIR4_S5


title: KIR4_S499


title: KIR4_S500


title: KIR4_S59


title: KIR4_S501


title: KIR4_S497


title: KIR4_S496


title: KIR4_S498


title: KIR4_S494


title: KIR4_S495


title: KIR4_S493


title: KIR4_S492


title: KIR4_S515


title: KIR4_S491


title: KIR4_S490


title: KIR4_S487


title: KIR4_S489


title: KIR4_S49


title: KIR4_S485


title: KIR4_S488


title: KIR4_S486


title: KIR4_S483


title: KIR4_S484


title: KIR4_S478


title: KIR4_S481


title: KIR4_S48


title: KIR4_S476


title: KIR4_S473


title: KIR4_S474


title: KIR4_S472


title: KIR4_S50


title: KIR4_S470


title: KIR4_S47


title: KIR4_S46


title: KIR4_S45


title: KIR4_S43


title: KIR4_S423


title: KIR4_S422


title: KIR4_S44


title: KIR4_S421


title: KIR4_S42


title: KIR4_S417


title: KIR4_S419


title: KIR4_S413


title: KIR4_S414


title: KIR4_S418


title: KIR4_S412


title: KIR4_S415


title: KIR4_S420


title: KIR4_S477


title: KIR4_S411


title: KIR4_S475


title: KIR4_S471


title: KIR4_S408


title: KIR4_S406


title: KIR4_S41


title: KIR4_S482


title: KIR4_S404


title: KIR4_S405


title: KIR4_S407


title: KIR4_S410


title: KIR4_S409


title: KIR4_S403


title: KIR4_S400


title: KIR4_S40


title: KIR4_S4


title: KIR4_S396


title: KIR4_S399


title: KIR4_S394


title: KIR4_S395


title: KIR4_S392


title: KIR4_S391


title: KIR4_S416


title: KIR4_S398


title: KIR4_S389


title: KIR4_S386


title: KIR4_S390


title: KIR4_S383


title: KIR4_S39


title: KIR4_S388


title: KIR4_S381


title: KIR4_S384


title: KIR4_S380


title: KIR4_S385


title: KIR4_S38


title: KIR4_S387


title: KIR4_S379


title: KIR4_S377


title: KIR4_S402


title: KIR4_S376


title: KIR4_S36


title: KIR4_S37


title: KIR4_S401


title: KIR4_S35


title: KIR4_S34


title: KIR4_S32


title: KIR4_S397


title: KIR4_S393


title: KIR4_S3


title: KIR4_S31


title: KIR4_S29


title: KIR4_S28


title: KIR4_S26


title: KIR4_S22


title: KIR4_S24


title: KIR4_S25


title: KIR4_S23


title: KIR4_S20


title: KIR4_S2


title: KIR4_S21


title: KIR4_S188


title: KIR4_S19


title: KIR4_S187


title: KIR4_S378


title: KIR4_S186


title: KIR4_S183


title: KIR4_S182


title: KIR4_S185


title: KIR4_S184


title: KIR4_S181


title: KIR4_S18


title: KIR4_S33


title: KIR4_S179


title: KIR4_S30


title: KIR4_S176


title: KIR4_S178


title: KIR4_S172


title: KIR4_S180


title: KIR4_S27


title: KIR4_S174


title: KIR4_S173


title: KIR4_S177


title: KIR4_S175


title: KIR4_S166


title: KIR4_S170


title: KIR4_S167


title: KIR4_S17


title: KIR4_S164


title: KIR4_S163


title: KIR4_S169


title: KIR4_S168


title: KIR4_S161


title: KIR4_S165


title: KIR4_S162


title: KIR4_S16


title: KIR4_S160


title: KIR4_S159


title: KIR4_S158


title: KIR4_S157


title: KIR4_S153


title: KIR4_S152


title: KIR4_S154


title: KIR4_S151


title: KIR4_S156


title: KIR4_S155


title: KIR4_S150


title: KIR4_S171


title: KIR4_S142


title: KIR4_S147


title: KIR4_S149


title: KIR4_S15


title: KIR4_S146


title: KIR4_S144


title: KIR4_S141


title: KIR4_S145


title: KIR4_S143


title: KIR4_S139


title: KIR4_S140


title: KIR4_S138


title: KIR4_S14


title: KIR4_S136


title: KIR4_S133


title: KIR4_S135


title: KIR4_S134


title: KIR4_S132


title: KIR4_S131


title: KIR4_S130


title: KIR4_S13


title: KIR4_S129


title: KIR4_S128


title: KIR4_S126


title: KIR4_S124


title: KIR4_S127


title: KIR4_S148


title: KIR4_S123


title: KIR4_S125


title: KIR4_S122


title: KIR4_S121


title: KIR4_S115


title: KIR4_S116


title: KIR4_S118


title: KIR4_S113


title: KIR4_S117


title: KIR4_S112


title: KIR4_S110


title: KIR4_S114


title: KIR4_S119


title: KIR4_S11


title: KIR4_S109


title: KIR4_S120


title: KIR4_S108


title: KIR4_S107


title: KIR4_S137


title: KIR4_S106


title: KIR4_S105


title: KIR4_S104


title: KIR4_S103


title: KIR4_S102


title: KIR4_S101


title: KIR4_S100


title: KIR3_S98


title: KIR4_S10


title: KIR4_S1


title: KIR3_S97


title: KIR4_S12


title: KIR3_S94


title: KIR3_S93


title: KIR3_S91


title: KIR3_S9


title: KIR3_S89


title: KIR3_S87


title: KIR3_S92


title: KIR4_S111


title: KIR3_S85


title: KIR3_S90


title: KIR3_S83


title: KIR3_S8


title: KIR3_S81


title: KIR3_S86


title: KIR3_S82


title: KIR3_S80


title: KIR3_S78


title: KIR3_S76


title: KIR3_S77


title: KIR3_S748


title: KIR3_S749


title: KIR3_S752


title: KIR3_S747


title: KIR3_S746


title: KIR3_S745


title: KIR3_S743


title: KIR3_S740


title: KIR3_S739


title: KIR3_S734


title: KIR3_S74


title: KIR3_S733


title: KIR3_S735


title: KIR3_S737


title: KIR3_S738


title: KIR3_S88


title: KIR3_S730


title: KIR3_S84


title: KIR3_S732


title: KIR3_S729


title: KIR3_S736


title: KIR3_S73


title: KIR3_S731


title: KIR3_S79


title: KIR3_S728


title: KIR3_S726


title: KIR3_S753


title: KIR3_S727


title: KIR3_S724


title: KIR3_S725


title: KIR3_S723


title: KIR3_S75


title: KIR3_S722


title: KIR3_S721


title: KIR3_S720


title: KIR3_S72


title: KIR3_S719


title: KIR3_S742


title: KIR3_S717


title: KIR3_S716


title: KIR3_S715


title: KIR3_S718


title: KIR3_S714


title: KIR3_S710


title: KIR3_S712


title: KIR3_S713


title: KIR3_S711


title: KIR3_S71


title: KIR3_S708


title: KIR3_S707


title: KIR3_S706


title: KIR3_S705


title: KIR3_S709


title: KIR3_S704


title: KIR3_S701


title: KIR3_S703


title: KIR3_S702


title: KIR3_S700


title: KIR3_S7


title: KIR3_S70


title: KIR3_S698


title: KIR3_S695


title: KIR3_S696


title: KIR3_S697


title: KIR3_S694


title: KIR3_S692


title: KIR3_S69


title: KIR3_S688


title: KIR3_S687


title: KIR3_S683


title: KIR3_S689


title: KIR3_S686


title: KIR3_S685


title: KIR3_S684


title: KIR3_S680


title: KIR3_S676


title: KIR3_S682


title: KIR3_S677


title: KIR3_S678


title: KIR3_S674


title: KIR3_S681


title: KIR3_S672


title: KIR3_S675


title: KIR3_S679


title: KIR3_S671


title: KIR3_S699


title: KIR3_S67


title: KIR3_S673


title: KIR3_S668


title: KIR3_S670


title: KIR3_S693


title: KIR3_S666


title: KIR3_S691


title: KIR3_S690


title: KIR3_S665


title: KIR3_S664


title: KIR3_S66


title: KIR3_S660


title: KIR3_S662


title: KIR3_S663


title: KIR3_S659


title: KIR3_S661


title: KIR3_S658


title: KIR3_S657


title: KIR3_S654


title: KIR3_S68


title: KIR3_S651


title: KIR3_S655


title: KIR3_S650


title: KIR3_S652


title: KIR3_S653


title: KIR3_S656


title: KIR3_S65


title: KIR3_S649


title: KIR3_S643


title: KIR3_S648


title: KIR3_S645


title: KIR3_S644


title: KIR3_S647


title: KIR3_S667


title: KIR3_S642


title: KIR3_S641


title: KIR3_S640


title: KIR3_S64


title: KIR3_S639


title: KIR3_S63


title: KIR3_S60


title: KIR3_S584


title: KIR3_S578


title: KIR3_S581


title: KIR3_S575


title: KIR3_S573


title: KIR3_S577


title: KIR3_S58


title: KIR3_S571


title: KIR3_S57


title: KIR3_S568


title: KIR3_S579


title: KIR3_S576


title: KIR3_S567


title: KIR3_S569


title: KIR3_S570


title: KIR3_S566


title: KIR3_S564


title: KIR3_S565


title: KIR3_S563


title: KIR3_S561


title: KIR3_S562


title: KIR3_S560


title: KIR3_S56


title: KIR3_S559


title: KIR3_S557


title: KIR3_S61


title: KIR3_S62


title: KIR3_S6


title: KIR3_S582


title: KIR3_S551


title: KIR3_S556


title: KIR3_S554


title: KIR3_S552


title: KIR3_S572


title: KIR3_S553


title: KIR3_S555


title: KIR3_S549


title: KIR3_S548


title: KIR3_S547


title: KIR3_S543


title: KIR3_S546


title: KIR3_S544


title: KIR3_S54


title: KIR3_S542


title: KIR3_S541


title: KIR3_S540


title: KIR3_S538


title: KIR3_S539


title: KIR3_S537


title: KIR3_S534


title: KIR3_S533


title: KIR3_S531


title: KIR3_S529


title: KIR3_S526


title: KIR3_S524


title: KIR3_S523


title: KIR3_S522


title: KIR3_S530


title: KIR3_S521


title: KIR3_S545


title: KIR3_S528


title: KIR3_S52


title: KIR3_S53


title: KIR3_S527


title: KIR3_S550


title: KIR3_S532


title: KIR3_S519


title: KIR3_S520


title: KIR3_S55


title: KIR3_S517


title: KIR3_S518


title: KIR3_S514


title: KIR3_S516


title: KIR3_S513


title: KIR3_S512


title: KIR3_S511


title: KIR3_S51


title: KIR3_S535


title: KIR3_S536


title: KIR3_S509


title: KIR3_S507


title: KIR3_S508


title: KIR3_S506


title: KIR3_S503


title: KIR3_S504


title: KIR3_S501


title: KIR3_S500


title: KIR3_S525


title: KIR3_S499


title: KIR3_S502


title: KIR3_S497


title: KIR3_S495


title: KIR3_S493


title: KIR3_S515


title: KIR3_S494


title: KIR3_S49


title: KIR3_S489


title: KIR3_S487


title: KIR3_S496


title: KIR3_S488


title: KIR3_S485


title: KIR3_S490


title: KIR3_S486


title: KIR3_S484


title: KIR3_S483


title: KIR3_S481


title: KIR3_S482


title: KIR3_S48


title: KIR3_S478


title: KIR3_S477


title: KIR3_S473


title: KIR3_S492


title: KIR3_S474


title: KIR3_S475


title: KIR3_S50


title: KIR3_S470


title: KIR3_S472


title: KIR3_S476


title: KIR3_S498


title: KIR3_S471


title: KIR3_S469


title: KIR3_S47


title: KIR3_S466


title: KIR3_S467


title: KIR3_S463


title: KIR3_S468


title: KIR3_S462


title: KIR3_S461


title: KIR3_S460


title: KIR3_S46


title: KIR3_S459


title: KIR3_S457


title: KIR3_S455


title: KIR3_S456


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title: KIR3_S452


title: KIR3_S451


title: KIR3_S450


title: KIR3_S45


title: KIR3_S448


title: KIR3_S447


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title: KIR3_S445


title: KIR3_S443


title: KIR3_S444


title: KIR3_S442


title: KIR3_S44


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title: KIR3_S441


title: KIR3_S437


title: KIR3_S434


title: KIR3_S432


title: KIR3_S435


title: KIR3_S431


title: KIR3_S430


title: KIR3_S43


title: KIR3_S429


title: KIR3_S428


title: KIR3_S458


title: KIR3_S426


title: KIR3_S427


title: KIR3_S425


title: KIR3_S424


title: KIR3_S422


title: KIR3_S423


title: KIR3_S421


title: KIR3_S419


title: KIR3_S420


title: KIR3_S42


title: KIR3_S418


title: KIR3_S416


title: KIR3_S417


title: KIR3_S415


title: KIR3_S414


title: KIR3_S413


title: KIR3_S412


title: KIR3_S411


title: KIR3_S41


title: KIR3_S409


title: KIR3_S407


title: KIR3_S408


title: KIR3_S403


title: KIR3_S406


title: KIR3_S405


title: KIR3_S436


title: KIR3_S433


title: KIR3_S402


title: KIR3_S40


title: KIR3_S400


title: KIR3_S4


title: KIR3_S401


title: KIR3_S398


title: KIR3_S395


title: KIR3_S397


title: KIR3_S39


title: KIR3_S385


title: KIR3_S384


title: KIR3_S383


title: KIR3_S381


title: KIR3_S38


title: KIR3_S379


title: KIR3_S378


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title: KIR3_S376


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title: KIR3_S370


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title: KIR3_S37


title: KIR3_S373


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title: KIR3_S365


title: KIR3_S404


title: KIR3_S364


title: KIR3_S363


title: KIR3_S399


title: KIR3_S361


title: KIR3_S359


title: KIR3_S362


title: KIR3_S356


title: KIR3_S357


title: KIR3_S36


title: KIR3_S358


title: KIR3_S394


title: KIR3_S396


title: KIR3_S355


title: KIR3_S380


title: KIR3_S352


title: KIR3_S377


title: KIR3_S349


title: KIR3_S354


title: KIR3_S35


title: KIR3_S350


title: KIR3_S353


title: KIR3_S348


title: KIR3_S341


title: KIR3_S351


title: KIR3_S347


title: KIR3_S344


title: KIR3_S34


title: KIR3_S360


title: KIR3_S343


title: KIR3_S346


title: KIR3_S338


title: KIR3_S337


title: KIR3_S336


title: KIR3_S335


title: KIR3_S342


title: KIR3_S340


title: KIR3_S334


title: KIR3_S339


title: KIR3_S330


title: KIR3_S329


title: KIR3_S333


title: KIR3_S331


title: KIR3_S327


title: KIR3_S321


title: KIR3_S325


title: KIR3_S326


title: KIR3_S317


title: KIR3_S319


title: KIR3_S315


title: KIR3_S324


title: KIR3_S312


title: KIR3_S320


title: KIR3_S318


title: KIR3_S322


title: KIR3_S316


title: KIR3_S323


title: KIR3_S308


title: KIR3_S313


title: KIR3_S305


title: KIR3_S31


title: KIR3_S307


title: KIR3_S302


title: KIR3_S311


title: KIR3_S33


title: KIR3_S309


title: KIR3_S301


title: KIR3_S30


title: KIR3_S3


title: KIR3_S297


title: KIR3_S332


title: KIR3_S304


title: KIR3_S306


title: KIR3_S300


title: KIR3_S290


title: KIR3_S295


title: KIR3_S296


title: KIR3_S303


title: KIR3_S310


title: KIR3_S287


title: KIR3_S328


title: KIR3_S32


title: KIR3_S299


title: KIR3_S280


title: KIR3_S294


title: KIR3_S292


title: KIR3_S293


title: KIR3_S298


title: KIR3_S283


title: KIR3_S278


title: KIR3_S276


title: KIR3_S27


title: KIR3_S286


title: KIR3_S285


title: KIR3_S282


title: KIR3_S29


title: KIR3_S289


title: KIR3_S284


title: KIR3_S268


title: KIR3_S281


title: KIR3_S265


title: KIR3_S263


title: KIR3_S264


title: KIR3_S259


title: KIR3_S26


title: KIR3_S279


title: KIR3_S28


title: KIR3_S273


title: KIR3_S271


title: KIR3_S270


title: KIR3_S274


title: KIR3_S266


title: KIR3_S269


title: KIR3_S261


title: KIR3_S277


title: KIR3_S257


title: KIR3_S256


title: KIR3_S258


title: KIR3_S255


title: KIR3_S253


title: KIR3_S251


title: KIR3_S272


title: KIR3_S254


title: KIR3_S25