title: Young_2, Cell 74 of 384 [AS-440976-LR-48372]


title: Young_2, Cell 72 of 384 [AS-440972-LR-48372]

status: Public on Apr 27 2022

type: SRA

city: Ulm

first: Medhanie

middle: Assmelash

last: Mulaw

address: Albert-Einstein-Allee 11

country: Germany

institute: University of Ulm

library_source: transcriptomic

data_processing:
Single-end raw reads were aligned to the reference genome using HISAT2
Gene counts were obtained using HTSeq using Mus_musculus.GRCm38.99.gtf
Data matrix count table from individual count files was generated using R and Bioconductor
Assembly: Mus_musculus build GRCm38.99
Supplementary files format and content: tab-delimited text of raw gene expression count matrix

molecule: total RNA

organism: Mus musculus

source_name: Lgr5 high-ISCs

growth_protocol: Lgr5 high-ISCs were sorted in 384 well low bind plates (Eppendorf) containing 1.2 µl lysis buffer (0.1% triton (Sigma-Aldrich), 1U/μl RNase Inhibitor (Takara Bio), 2.5 µM polyT primer (Thermo Fisher Scientific), 2.5 mM dNTPs (Thermo Fisher Scientific)). After sorting the plates spun down and immediately frozen at -80 °C for further processes.

extract_protocol: SMARTER mRNA-Seq: RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.

tag: cell type

value: Lgr5 high-ISCs

tag: sample group

tag: genotype


title: Young_2, Cell 73 of 384 [AS-440974-LR-48372]


title: Young_2, Cell 70 of 384 [AS-440968-LR-48372]


title: Young_2, Cell 71 of 384 [AS-440970-LR-48372]


title: Young_2, Cell 65 of 384 [AS-440958-LR-48372]


title: Young_2, Cell 97 of 384 [AS-441022-LR-48372]


title: Young_2, Cell 66 of 384 [AS-440960-LR-48372]


title: Young_2, Cell 67 of 384 [AS-440962-LR-48372]


title: Young_2, Cell 68 of 384 [AS-440964-LR-48372]


title: Young_2, Cell 87 of 384 [AS-441002-LR-48372]


title: Young_2, Cell 145 of 384 [AS-441118-LR-48372]


title: Young_2, Cell 62 of 384 [AS-440952-LR-48372]


title: Young_2, Cell 80 of 384 [AS-440988-LR-48372]


title: Young_2, Cell 63 of 384 [AS-440954-LR-48372]


title: Young_2, Cell 64 of 384 [AS-440956-LR-48372]


title: Young_2, Cell 59 of 384 [AS-440946-LR-48372]


title: Young_2, Cell 60 of 384 [AS-440948-LR-48372]


title: Young_2, Cell 61 of 384 [AS-440950-LR-48372]


title: Young_2, Cell 76 of 384 [AS-440980-LR-48372]


title: Young_2, Cell 58 of 384 [AS-440944-LR-48372]


title: Young_2, Cell 55 of 384 [AS-440938-LR-48372]


title: Young_2, Cell 54 of 384 [AS-440936-LR-48372]


title: Young_2, Cell 53 of 384 [AS-440934-LR-48372]


title: Young_2, Cell 51 of 384 [AS-440930-LR-48372]


title: Young_2, Cell 50 of 384 [AS-440928-LR-48372]


title: Young_2, Cell 52 of 384 [AS-440932-LR-48372]


title: Young_2, Cell 47 of 384 [AS-440922-LR-48372]


title: Young_2, Cell 45 of 384 [AS-440918-LR-48372]


title: Young_2, Cell 46 of 384 [AS-440920-LR-48372]


title: Young_2, Cell 69 of 384 [AS-440966-LR-48372]


title: Young_2, Cell 49 of 384 [AS-440926-LR-48372]


title: Young_2, Cell 43 of 384 [AS-440914-LR-48372]


title: Young_2, Cell 44 of 384 [AS-440916-LR-48372]


title: Young_2, Cell 42 of 384 [AS-440912-LR-48372]


title: Young_2, Cell 41 of 384 [AS-440910-LR-48372]


title: Young_2, Cell 38 of 384 [AS-440904-LR-48372]


title: Young_2, Cell 34 of 384 [AS-440896-LR-48372]


title: Young_2, Cell 33 of 384 [AS-440894-LR-48372]


title: Young_2, Cell 32 of 384 [AS-440892-LR-48372]


title: Young_2, Cell 30 of 384 [AS-440888-LR-48372]


title: Young_2, Cell 31 of 384 [AS-440890-LR-48372]


title: Young_2, Cell 56 of 384 [AS-440940-LR-48372]


title: Young_2, Cell 57 of 384 [AS-440942-LR-48372]


title: Young_2, Cell 82 of 384 [AS-440992-LR-48372]


title: Young_2, Cell 28 of 384 [AS-440884-LR-48372]


title: Young_2, Cell 29 of 384 [AS-440886-LR-48372]


title: Young_2, Cell 27 of 384 [AS-440882-LR-48372]


title: Young_2, Cell 48 of 384 [AS-440924-LR-48372]


title: Young_2, Cell 26 of 384 [AS-440880-LR-48372]


title: Young_2, Cell 23 of 384 [AS-440874-LR-48372]


title: Young_2, Cell 25 of 384 [AS-440878-LR-48372]


title: Young_2, Cell 22 of 384 [AS-440872-LR-48372]


title: Young_2, Cell 24 of 384 [AS-440876-LR-48372]


title: Young_2, Cell 20 of 384 [AS-440868-LR-48372]


title: Young_2, Cell 21 of 384 [AS-440870-LR-48372]


title: Young_2, Cell 19 of 384 [AS-440866-LR-48372]


title: Young_2, Cell 15 of 384 [AS-440858-LR-48372]


title: Young_2, Cell 17 of 384 [AS-440862-LR-48372]


title: Young_2, Cell 16 of 384 [AS-440860-LR-48372]


title: Young_2, Cell 18 of 384 [AS-440864-LR-48372]


title: Young_2, Cell 14 of 384 [AS-440856-LR-48372]


title: Young_2, Cell 12 of 384 [AS-440852-LR-48372]


title: Young_2, Cell 11 of 384 [AS-440850-LR-48372]


title: Young_2, Cell 39 of 384 [AS-440906-LR-48372]


title: Young_2, Cell 35 of 384 [AS-440898-LR-48372]


title: Young_2, Cell 37 of 384 [AS-440902-LR-48372]


title: Young_2, Cell 10 of 384 [AS-440848-LR-48372]


title: Young_2, Cell 8 of 384 [AS-440844-LR-48372]


title: Young_2, Cell 9 of 384 [AS-440846-LR-48372]


title: Young_2, Cell 6 of 384 [AS-440840-LR-48372]


title: Young_2, Cell 7 of 384 [AS-440842-LR-48372]


title: Young_2, Cell 5 of 384 [AS-440838-LR-48372]


title: Young_2, Cell 4 of 384 [AS-440836-LR-48372]


title: Young_2, Cell 36 of 384 [AS-440900-LR-48372]


title: Young_2, Cell 3 of 384 [AS-440834-LR-48372]


title: Young_2, Cell 1 of 384 [AS-440830-LR-48372]


title: Young_1, Cell 190 of 192 [AS-440439-LR-48373]


title: Young_1, Cell 187 of 192 [AS-440433-LR-48373]


title: Young_1, Cell 189 of 192 [AS-440437-LR-48373]


title: Young_1, Cell 191 of 192 [AS-440441-LR-48373]


title: Young_1, Cell 186 of 192 [AS-440431-LR-48373]


title: Young_1, Cell 188 of 192 [AS-440435-LR-48373]


title: Young_1, Cell 183 of 192 [AS-440425-LR-48373]


title: Young_1, Cell 192 of 192 [AS-440443-LR-48373]


title: Young_1, Cell 185 of 192 [AS-440429-LR-48373]


title: Young_1, Cell 184 of 192 [AS-440427-LR-48373]


title: Young_1, Cell 181 of 192 [AS-440421-LR-48373]


title: Young_1, Cell 177 of 192 [AS-440413-LR-48373]


title: Young_1, Cell 178 of 192 [AS-440415-LR-48373]


title: Young_1, Cell 176 of 192 [AS-440411-LR-48373]


title: Young_2, Cell 13 of 384 [AS-440854-LR-48372]


title: Young_1, Cell 180 of 192 [AS-440419-LR-48373]


title: Young_1, Cell 174 of 192 [AS-440407-LR-48373]


title: Young_1, Cell 170 of 192 [AS-440399-LR-48373]


title: Young_1, Cell 171 of 192 [AS-440401-LR-48373]


title: Young_1, Cell 168 of 192 [AS-440395-LR-48373]


title: Young_1, Cell 173 of 192 [AS-440405-LR-48373]


title: Young_1, Cell 175 of 192 [AS-440409-LR-48373]


title: Young_1, Cell 172 of 192 [AS-440403-LR-48373]


title: Young_1, Cell 169 of 192 [AS-440397-LR-48373]


title: Young_1, Cell 167 of 192 [AS-440393-LR-48373]


title: Young_1, Cell 165 of 192 [AS-440389-LR-48373]


title: Young_1, Cell 163 of 192 [AS-440385-LR-48373]


title: Young_2, Cell 2 of 384 [AS-440832-LR-48372]


title: Young_1, Cell 164 of 192 [AS-440387-LR-48373]


title: Young_1, Cell 162 of 192 [AS-440383-LR-48373]


title: Young_1, Cell 161 of 192 [AS-440381-LR-48373]


title: Young_1, Cell 159 of 192 [AS-440377-LR-48373]


title: Young_1, Cell 160 of 192 [AS-440379-LR-48373]


title: Young_1, Cell 182 of 192 [AS-440423-LR-48373]


title: Young_1, Cell 158 of 192 [AS-440375-LR-48373]


title: Young_1, Cell 157 of 192 [AS-440373-LR-48373]


title: Young_1, Cell 166 of 192 [AS-440391-LR-48373]


title: Young_1, Cell 156 of 192 [AS-440371-LR-48373]


title: Young_1, Cell 151 of 192 [AS-440361-LR-48373]


title: Young_1, Cell 154 of 192 [AS-440367-LR-48373]


title: Young_1, Cell 153 of 192 [AS-440365-LR-48373]


title: Young_1, Cell 150 of 192 [AS-440359-LR-48373]


title: Young_1, Cell 152 of 192 [AS-440363-LR-48373]


title: Young_1, Cell 155 of 192 [AS-440369-LR-48373]


title: Young_1, Cell 149 of 192 [AS-440357-LR-48373]


title: Young_1, Cell 145 of 192 [AS-440349-LR-48373]


title: Young_1, Cell 143 of 192 [AS-440345-LR-48373]


title: Young_1, Cell 147 of 192 [AS-440353-LR-48373]


title: Young_1, Cell 179 of 192 [AS-440417-LR-48373]


title: Young_1, Cell 144 of 192 [AS-440347-LR-48373]


title: Young_1, Cell 146 of 192 [AS-440351-LR-48373]


title: Young_1, Cell 148 of 192 [AS-440355-LR-48373]


title: Young_1, Cell 141 of 192 [AS-440341-LR-48373]


title: Young_1, Cell 142 of 192 [AS-440343-LR-48373]


title: Young_1, Cell 140 of 192 [AS-440339-LR-48373]


title: Young_1, Cell 138 of 192 [AS-440335-LR-48373]


title: Young_1, Cell 137 of 192 [AS-440333-LR-48373]


title: Young_1, Cell 139 of 192 [AS-440337-LR-48373]


title: Young_1, Cell 135 of 192 [AS-440329-LR-48373]


title: Young_1, Cell 134 of 192 [AS-440327-LR-48373]


title: Young_1, Cell 136 of 192 [AS-440331-LR-48373]


title: Young_1, Cell 133 of 192 [AS-440325-LR-48373]


title: Young_1, Cell 132 of 192 [AS-440323-LR-48373]


title: Young_1, Cell 131 of 192 [AS-440321-LR-48373]


title: Young_1, Cell 129 of 192 [AS-440317-LR-48373]


title: Young_1, Cell 128 of 192 [AS-440315-LR-48373]


title: Young_1, Cell 125 of 192 [AS-440309-LR-48373]


title: Young_1, Cell 127 of 192 [AS-440313-LR-48373]


title: Young_1, Cell 122 of 192 [AS-440303-LR-48373]


title: Young_1, Cell 124 of 192 [AS-440307-LR-48373]


title: Young_1, Cell 130 of 192 [AS-440319-LR-48373]


title: Young_1, Cell 123 of 192 [AS-440305-LR-48373]


title: Young_1, Cell 126 of 192 [AS-440311-LR-48373]


title: Young_1, Cell 120 of 192 [AS-440299-LR-48373]


title: Young_1, Cell 121 of 192 [AS-440301-LR-48373]


title: Young_3, Cell 381 of 384 [AS-443937-LR-48446]


title: Young_3, Cell 380 of 384 [AS-443935-LR-48446]


title: Young_3, Cell 382 of 384 [AS-443939-LR-48446]


title: Young_3, Cell 384 of 384 [AS-443943-LR-48446]


title: Young_3, Cell 379 of 384 [AS-443933-LR-48446]


title: Young_3, Cell 383 of 384 [AS-443941-LR-48446]


title: Young_3, Cell 371 of 384 [AS-443917-LR-48446]


title: Young_3, Cell 375 of 384 [AS-443925-LR-48446]


title: Young_3, Cell 370 of 384 [AS-443915-LR-48446]


title: Young_3, Cell 372 of 384 [AS-443919-LR-48446]


title: Young_3, Cell 378 of 384 [AS-443931-LR-48446]


title: Young_3, Cell 368 of 384 [AS-443911-LR-48446]


title: Young_3, Cell 374 of 384 [AS-443923-LR-48446]


title: Young_3, Cell 367 of 384 [AS-443909-LR-48446]


title: Young_3, Cell 360 of 384 [AS-443895-LR-48446]


title: Young_3, Cell 365 of 384 [AS-443905-LR-48446]


title: Young_3, Cell 359 of 384 [AS-443893-LR-48446]


title: Young_3, Cell 358 of 384 [AS-443891-LR-48446]


title: Young_3, Cell 363 of 384 [AS-443901-LR-48446]


title: Young_3, Cell 366 of 384 [AS-443907-LR-48446]


title: Young_3, Cell 376 of 384 [AS-443927-LR-48446]


title: Young_3, Cell 369 of 384 [AS-443913-LR-48446]


title: Young_3, Cell 361 of 384 [AS-443897-LR-48446]


title: Young_3, Cell 355 of 384 [AS-443885-LR-48446]


title: Young_3, Cell 354 of 384 [AS-443883-LR-48446]


title: Young_3, Cell 356 of 384 [AS-443887-LR-48446]


title: Young_3, Cell 364 of 384 [AS-443903-LR-48446]


title: Young_3, Cell 351 of 384 [AS-443877-LR-48446]


title: Young_3, Cell 350 of 384 [AS-443875-LR-48446]


title: Young_3, Cell 373 of 384 [AS-443921-LR-48446]


title: Young_3, Cell 362 of 384 [AS-443899-LR-48446]


title: Young_3, Cell 346 of 384 [AS-443867-LR-48446]


title: Young_3, Cell 344 of 384 [AS-443863-LR-48446]


title: Young_3, Cell 343 of 384 [AS-443861-LR-48446]


title: Young_3, Cell 348 of 384 [AS-443871-LR-48446]


title: Young_3, Cell 377 of 384 [AS-443929-LR-48446]


title: Young_3, Cell 345 of 384 [AS-443865-LR-48446]


title: Young_3, Cell 341 of 384 [AS-443857-LR-48446]


title: Young_3, Cell 349 of 384 [AS-443873-LR-48446]


title: Young_3, Cell 340 of 384 [AS-443855-LR-48446]


title: Young_3, Cell 353 of 384 [AS-443881-LR-48446]


title: Young_3, Cell 338 of 384 [AS-443851-LR-48446]


title: Young_3, Cell 352 of 384 [AS-443879-LR-48446]


title: Young_3, Cell 339 of 384 [AS-443853-LR-48446]


title: Young_3, Cell 347 of 384 [AS-443869-LR-48446]


title: Young_3, Cell 333 of 384 [AS-443841-LR-48446]


title: Young_3, Cell 342 of 384 [AS-443859-LR-48446]


title: Young_3, Cell 330 of 384 [AS-443835-LR-48446]


title: Young_3, Cell 336 of 384 [AS-443847-LR-48446]


title: Young_3, Cell 329 of 384 [AS-443833-LR-48446]


title: Young_3, Cell 328 of 384 [AS-443831-LR-48446]


title: Young_3, Cell 327 of 384 [AS-443829-LR-48446]


title: Young_3, Cell 337 of 384 [AS-443849-LR-48446]


title: Young_3, Cell 334 of 384 [AS-443843-LR-48446]


title: Young_3, Cell 331 of 384 [AS-443837-LR-48446]


title: Young_3, Cell 326 of 384 [AS-443827-LR-48446]


title: Young_3, Cell 357 of 384 [AS-443889-LR-48446]


title: Young_3, Cell 325 of 384 [AS-443825-LR-48446]


title: Young_3, Cell 324 of 384 [AS-443823-LR-48446]


title: Young_3, Cell 322 of 384 [AS-443819-LR-48446]


title: Young_3, Cell 323 of 384 [AS-443821-LR-48446]


title: Young_3, Cell 320 of 384 [AS-443815-LR-48446]


title: Young_3, Cell 321 of 384 [AS-443817-LR-48446]


title: Young_3, Cell 319 of 384 [AS-443813-LR-48446]


title: Young_3, Cell 315 of 384 [AS-443805-LR-48446]


title: Young_3, Cell 317 of 384 [AS-443809-LR-48446]


title: Young_3, Cell 316 of 384 [AS-443807-LR-48446]


title: Young_3, Cell 313 of 384 [AS-443801-LR-48446]


title: Young_3, Cell 314 of 384 [AS-443803-LR-48446]


title: Young_3, Cell 312 of 384 [AS-443799-LR-48446]


title: Young_3, Cell 311 of 384 [AS-443797-LR-48446]


title: Young_3, Cell 310 of 384 [AS-443795-LR-48446]


title: Young_3, Cell 308 of 384 [AS-443791-LR-48446]


title: Young_3, Cell 335 of 384 [AS-443845-LR-48446]


title: Young_3, Cell 332 of 384 [AS-443839-LR-48446]


title: Young_3, Cell 303 of 384 [AS-443781-LR-48446]


title: Young_3, Cell 302 of 384 [AS-443779-LR-48446]


title: Young_3, Cell 306 of 384 [AS-443787-LR-48446]


title: Young_3, Cell 304 of 384 [AS-443783-LR-48446]


title: Young_3, Cell 301 of 384 [AS-443777-LR-48446]


title: Young_3, Cell 299 of 384 [AS-443773-LR-48446]


title: Young_3, Cell 300 of 384 [AS-443775-LR-48446]


title: Young_3, Cell 298 of 384 [AS-443771-LR-48446]


title: Young_3, Cell 296 of 384 [AS-443767-LR-48446]


title: Young_3, Cell 295 of 384 [AS-443765-LR-48446]


title: Young_3, Cell 294 of 384 [AS-443763-LR-48446]


title: Young_3, Cell 293 of 384 [AS-443761-LR-48446]


title: Young_3, Cell 290 of 384 [AS-443755-LR-48446]


title: Young_3, Cell 287 of 384 [AS-443749-LR-48446]


title: Young_3, Cell 288 of 384 [AS-443751-LR-48446]


title: Young_3, Cell 318 of 384 [AS-443811-LR-48446]


title: Young_3, Cell 286 of 384 [AS-443747-LR-48446]


title: Young_3, Cell 289 of 384 [AS-443753-LR-48446]


title: Young_3, Cell 309 of 384 [AS-443793-LR-48446]


title: Young_3, Cell 282 of 384 [AS-443739-LR-48446]


title: Young_3, Cell 283 of 384 [AS-443741-LR-48446]


title: Young_3, Cell 278 of 384 [AS-443731-LR-48446]


title: Young_3, Cell 277 of 384 [AS-443729-LR-48446]


title: Young_3, Cell 281 of 384 [AS-443737-LR-48446]


title: Young_3, Cell 280 of 384 [AS-443735-LR-48446]


title: Young_3, Cell 279 of 384 [AS-443733-LR-48446]


title: Young_3, Cell 276 of 384 [AS-443727-LR-48446]


title: Young_3, Cell 305 of 384 [AS-443785-LR-48446]


title: Young_3, Cell 307 of 384 [AS-443789-LR-48446]


title: Young_3, Cell 275 of 384 [AS-443725-LR-48446]


title: Young_3, Cell 273 of 384 [AS-443721-LR-48446]


title: Young_3, Cell 274 of 384 [AS-443723-LR-48446]


title: Young_3, Cell 297 of 384 [AS-443769-LR-48446]


title: Young_3, Cell 270 of 384 [AS-443715-LR-48446]


title: Young_3, Cell 271 of 384 [AS-443717-LR-48446]


title: Young_3, Cell 269 of 384 [AS-443713-LR-48446]


title: Young_3, Cell 292 of 384 [AS-443759-LR-48446]


title: Young_3, Cell 267 of 384 [AS-443709-LR-48446]


title: Young_3, Cell 291 of 384 [AS-443757-LR-48446]


title: Young_3, Cell 266 of 384 [AS-443707-LR-48446]


title: Young_3, Cell 265 of 384 [AS-443705-LR-48446]


title: Young_3, Cell 264 of 384 [AS-443703-LR-48446]


title: Young_3, Cell 263 of 384 [AS-443701-LR-48446]


title: Young_3, Cell 285 of 384 [AS-443745-LR-48446]


title: Young_3, Cell 261 of 384 [AS-443697-LR-48446]


title: Young_3, Cell 284 of 384 [AS-443743-LR-48446]


title: Young_3, Cell 262 of 384 [AS-443699-LR-48446]


title: Young_3, Cell 260 of 384 [AS-443695-LR-48446]


title: Young_3, Cell 255 of 384 [AS-443685-LR-48446]


title: Young_3, Cell 256 of 384 [AS-443687-LR-48446]


title: Young_3, Cell 257 of 384 [AS-443689-LR-48446]


title: Young_3, Cell 259 of 384 [AS-443693-LR-48446]


title: Young_3, Cell 258 of 384 [AS-443691-LR-48446]


title: Young_3, Cell 252 of 384 [AS-443679-LR-48446]


title: Young_3, Cell 248 of 384 [AS-443671-LR-48446]


title: Young_3, Cell 253 of 384 [AS-443681-LR-48446]


title: Young_3, Cell 254 of 384 [AS-443683-LR-48446]


title: Young_3, Cell 247 of 384 [AS-443669-LR-48446]


title: Young_3, Cell 251 of 384 [AS-443677-LR-48446]


title: Young_3, Cell 246 of 384 [AS-443667-LR-48446]


title: Young_3, Cell 249 of 384 [AS-443673-LR-48446]


title: Young_3, Cell 272 of 384 [AS-443719-LR-48446]


title: Young_3, Cell 242 of 384 [AS-443659-LR-48446]


title: Young_3, Cell 245 of 384 [AS-443665-LR-48446]


title: Young_3, Cell 241 of 384 [AS-443657-LR-48446]


title: Young_3, Cell 244 of 384 [AS-443663-LR-48446]


title: Young_3, Cell 243 of 384 [AS-443661-LR-48446]


title: Young_3, Cell 240 of 384 [AS-443655-LR-48446]


title: Young_3, Cell 239 of 384 [AS-443653-LR-48446]


title: Young_3, Cell 268 of 384 [AS-443711-LR-48446]


title: Young_3, Cell 235 of 384 [AS-443645-LR-48446]


title: Young_3, Cell 233 of 384 [AS-443641-LR-48446]


title: Young_3, Cell 236 of 384 [AS-443647-LR-48446]


title: Young_3, Cell 234 of 384 [AS-443643-LR-48446]


title: Young_3, Cell 229 of 384 [AS-443633-LR-48446]


title: Young_3, Cell 237 of 384 [AS-443649-LR-48446]


title: Young_3, Cell 232 of 384 [AS-443639-LR-48446]


title: Young_3, Cell 231 of 384 [AS-443637-LR-48446]


title: Young_3, Cell 230 of 384 [AS-443635-LR-48446]


title: Young_3, Cell 228 of 384 [AS-443631-LR-48446]


title: Young_1, Cell 119 of 192 [AS-440297-LR-48373]


title: Young_3, Cell 226 of 384 [AS-443627-LR-48446]


title: Young_1, Cell 117 of 192 [AS-440293-LR-48373]


title: Young_1, Cell 118 of 192 [AS-440295-LR-48373]


title: Young_3, Cell 227 of 384 [AS-443629-LR-48446]


title: Young_1, Cell 115 of 192 [AS-440289-LR-48373]


title: Young_3, Cell 250 of 384 [AS-443675-LR-48446]


title: Young_1, Cell 113 of 192 [AS-440285-LR-48373]


title: Young_1, Cell 112 of 192 [AS-440283-LR-48373]


title: Young_1, Cell 111 of 192 [AS-440281-LR-48373]


title: Young_1, Cell 116 of 192 [AS-440291-LR-48373]


title: Young_1, Cell 109 of 192 [AS-440277-LR-48373]


title: Young_1, Cell 107 of 192 [AS-440273-LR-48373]


title: Young_1, Cell 110 of 192 [AS-440279-LR-48373]


title: Young_1, Cell 108 of 192 [AS-440275-LR-48373]


title: Young_1, Cell 106 of 192 [AS-440271-LR-48373]


title: Young_1, Cell 104 of 192 [AS-440267-LR-48373]


title: Young_1, Cell 105 of 192 [AS-440269-LR-48373]


title: Young_3, Cell 238 of 384 [AS-443651-LR-48446]


title: Young_1, Cell 102 of 192 [AS-440263-LR-48373]


title: Young_1, Cell 99 of 192 [AS-440257-LR-48373]


title: Young_1, Cell 101 of 192 [AS-440261-LR-48373]


title: Young_1, Cell 100 of 192 [AS-440259-LR-48373]


title: Young_1, Cell 97 of 192 [AS-440253-LR-48373]


title: Young_1, Cell 96 of 192 [AS-440251-LR-48373]


title: Young_1, Cell 91 of 192 [AS-440241-LR-48373]


title: Young_1, Cell 94 of 192 [AS-440247-LR-48373]


title: Young_1, Cell 90 of 192 [AS-440239-LR-48373]


title: Young_1, Cell 92 of 192 [AS-440243-LR-48373]


title: Young_1, Cell 93 of 192 [AS-440245-LR-48373]


title: Young_1, Cell 89 of 192 [AS-440237-LR-48373]


title: Young_1, Cell 114 of 192 [AS-440287-LR-48373]


title: Young_1, Cell 88 of 192 [AS-440235-LR-48373]


title: Young_1, Cell 86 of 192 [AS-440231-LR-48373]


title: Young_1, Cell 87 of 192 [AS-440233-LR-48373]


title: Young_1, Cell 85 of 192 [AS-440229-LR-48373]


title: Young_1, Cell 83 of 192 [AS-440225-LR-48373]


title: Young_1, Cell 84 of 192 [AS-440227-LR-48373]


title: Young_1, Cell 82 of 192 [AS-440223-LR-48373]


title: Young_1, Cell 103 of 192 [AS-440265-LR-48373]


title: Young_1, Cell 78 of 192 [AS-440215-LR-48373]


title: Young_1, Cell 76 of 192 [AS-440211-LR-48373]


title: Young_1, Cell 77 of 192 [AS-440213-LR-48373]


title: Young_1, Cell 74 of 192 [AS-440207-LR-48373]


title: Young_1, Cell 73 of 192 [AS-440205-LR-48373]


title: Young_1, Cell 75 of 192 [AS-440209-LR-48373]


title: Young_1, Cell 98 of 192 [AS-440255-LR-48373]


title: Young_1, Cell 95 of 192 [AS-440249-LR-48373]


title: Young_1, Cell 71 of 192 [AS-440201-LR-48373]


title: Young_1, Cell 70 of 192 [AS-440199-LR-48373]


title: Young_1, Cell 68 of 192 [AS-440195-LR-48373]


title: Young_1, Cell 67 of 192 [AS-440193-LR-48373]


title: Young_1, Cell 66 of 192 [AS-440191-LR-48373]


title: Young_1, Cell 62 of 192 [AS-440183-LR-48373]


title: Young_1, Cell 65 of 192 [AS-440189-LR-48373]


title: Young_1, Cell 64 of 192 [AS-440187-LR-48373]


title: Young_1, Cell 60 of 192 [AS-440179-LR-48373]


title: Young_1, Cell 63 of 192 [AS-440185-LR-48373]


title: Young_1, Cell 81 of 192 [AS-440221-LR-48373]


title: Young_1, Cell 61 of 192 [AS-440181-LR-48373]


title: Young_1, Cell 79 of 192 [AS-440217-LR-48373]


title: Young_1, Cell 80 of 192 [AS-440219-LR-48373]


title: Young_1, Cell 58 of 192 [AS-440175-LR-48373]


title: Young_1, Cell 59 of 192 [AS-440177-LR-48373]


title: Young_1, Cell 57 of 192 [AS-440173-LR-48373]


title: Young_1, Cell 56 of 192 [AS-440171-LR-48373]


title: Young_1, Cell 72 of 192 [AS-440203-LR-48373]


title: Young_1, Cell 51 of 192 [AS-440161-LR-48373]


title: Young_1, Cell 50 of 192 [AS-440159-LR-48373]


title: Young_1, Cell 49 of 192 [AS-440157-LR-48373]


title: Young_1, Cell 54 of 192 [AS-440167-LR-48373]


title: Young_1, Cell 53 of 192 [AS-440165-LR-48373]


title: Young_1, Cell 69 of 192 [AS-440197-LR-48373]


title: Young_1, Cell 47 of 192 [AS-440153-LR-48373]


title: Young_1, Cell 52 of 192 [AS-440163-LR-48373]


title: Young_1, Cell 55 of 192 [AS-440169-LR-48373]


title: Young_1, Cell 48 of 192 [AS-440155-LR-48373]


title: Young_1, Cell 44 of 192 [AS-440147-LR-48373]


title: Young_1, Cell 45 of 192 [AS-440149-LR-48373]


title: Young_1, Cell 43 of 192 [AS-440145-LR-48373]


title: Young_1, Cell 42 of 192 [AS-440143-LR-48373]


title: Young_1, Cell 37 of 192 [AS-440133-LR-48373]


title: Young_1, Cell 38 of 192 [AS-440135-LR-48373]


title: Young_1, Cell 41 of 192 [AS-440141-LR-48373]


title: Young_1, Cell 40 of 192 [AS-440139-LR-48373]


title: Young_1, Cell 32 of 192 [AS-440123-LR-48373]


title: Young_1, Cell 34 of 192 [AS-440127-LR-48373]


title: Young_1, Cell 35 of 192 [AS-440129-LR-48373]


title: Young_1, Cell 29 of 192 [AS-440117-LR-48373]


title: Young_1, Cell 46 of 192 [AS-440151-LR-48373]


title: Young_1, Cell 33 of 192 [AS-440125-LR-48373]


title: Young_1, Cell 30 of 192 [AS-440119-LR-48373]


title: Young_1, Cell 27 of 192 [AS-440113-LR-48373]


title: Young_1, Cell 28 of 192 [AS-440115-LR-48373]


title: Young_1, Cell 24 of 192 [AS-440107-LR-48373]


title: Young_1, Cell 26 of 192 [AS-440111-LR-48373]


title: Young_1, Cell 22 of 192 [AS-440103-LR-48373]


title: Young_1, Cell 25 of 192 [AS-440109-LR-48373]


title: Young_1, Cell 23 of 192 [AS-440105-LR-48373]


title: Young_1, Cell 36 of 192 [AS-440131-LR-48373]


title: Young_1, Cell 21 of 192 [AS-440101-LR-48373]


title: Young_1, Cell 20 of 192 [AS-440099-LR-48373]


title: Young_1, Cell 39 of 192 [AS-440137-LR-48373]


title: Young_1, Cell 18 of 192 [AS-440095-LR-48373]


title: Young_1, Cell 16 of 192 [AS-440091-LR-48373]


title: Young_1, Cell 15 of 192 [AS-440089-LR-48373]


title: Young_1, Cell 17 of 192 [AS-440093-LR-48373]


title: Young_1, Cell 14 of 192 [AS-440087-LR-48373]


title: Young_1, Cell 13 of 192 [AS-440085-LR-48373]


title: Young_1, Cell 31 of 192 [AS-440121-LR-48373]


title: Young_1, Cell 19 of 192 [AS-440097-LR-48373]


title: Young_1, Cell 10 of 192 [AS-440079-LR-48373]


title: Young_1, Cell 11 of 192 [AS-440081-LR-48373]


title: Young_1, Cell 12 of 192 [AS-440083-LR-48373]


title: Young_1, Cell 9 of 192 [AS-440077-LR-48373]


title: Young_1, Cell 8 of 192 [AS-440075-LR-48373]


title: Young_1, Cell 6 of 192 [AS-440071-LR-48373]


title: Young_1, Cell 1 of 192 [AS-440061-LR-48373]


title: Young_1, Cell 5 of 192 [AS-440069-LR-48373]


title: Old_4, Cell 384 of 384 [AS-443150-LR-48395]


title: Young_1, Cell 3 of 192 [AS-440065-LR-48373]


title: Young_1, Cell 4 of 192 [AS-440067-LR-48373]


title: Young_1, Cell 7 of 192 [AS-440073-LR-48373]


title: Old_4, Cell 383 of 384 [AS-443148-LR-48395]


title: Young_1, Cell 2 of 192 [AS-440063-LR-48373]


title: Old_4, Cell 378 of 384 [AS-443138-LR-48395]


title: Old_4, Cell 379 of 384 [AS-443140-LR-48395]


title: Old_4, Cell 382 of 384 [AS-443146-LR-48395]


title: Old_4, Cell 377 of 384 [AS-443136-LR-48395]


title: Old_4, Cell 381 of 384 [AS-443144-LR-48395]


title: Old_4, Cell 380 of 384 [AS-443142-LR-48395]


title: Old_4, Cell 375 of 384 [AS-443132-LR-48395]


title: Old_4, Cell 372 of 384 [AS-443126-LR-48395]


title: Old_4, Cell 369 of 384 [AS-443120-LR-48395]


title: Old_4, Cell 376 of 384 [AS-443134-LR-48395]


title: Old_4, Cell 373 of 384 [AS-443128-LR-48395]


title: Old_4, Cell 371 of 384 [AS-443124-LR-48395]


title: Old_4, Cell 374 of 384 [AS-443130-LR-48395]


title: Old_4, Cell 368 of 384 [AS-443118-LR-48395]


title: Old_4, Cell 370 of 384 [AS-443122-LR-48395]


title: Old_4, Cell 365 of 384 [AS-443112-LR-48395]


title: Old_4, Cell 366 of 384 [AS-443114-LR-48395]


title: Old_4, Cell 367 of 384 [AS-443116-LR-48395]


title: Old_4, Cell 364 of 384 [AS-443110-LR-48395]


title: Old_4, Cell 363 of 384 [AS-443108-LR-48395]


title: Old_4, Cell 361 of 384 [AS-443104-LR-48395]


title: Old_4, Cell 359 of 384 [AS-443100-LR-48395]


title: Old_4, Cell 360 of 384 [AS-443102-LR-48395]


title: Old_4, Cell 358 of 384 [AS-443098-LR-48395]


title: Old_4, Cell 355 of 384 [AS-443092-LR-48395]


title: Old_4, Cell 356 of 384 [AS-443094-LR-48395]


title: Old_4, Cell 354 of 384 [AS-443090-LR-48395]


title: Old_4, Cell 362 of 384 [AS-443106-LR-48395]


title: Old_4, Cell 351 of 384 [AS-443084-LR-48395]


title: Old_4, Cell 357 of 384 [AS-443096-LR-48395]


title: Old_4, Cell 353 of 384 [AS-443088-LR-48395]


title: Old_4, Cell 352 of 384 [AS-443086-LR-48395]


title: Old_4, Cell 348 of 384 [AS-443078-LR-48395]


title: Old_4, Cell 349 of 384 [AS-443080-LR-48395]


title: Old_4, Cell 350 of 384 [AS-443082-LR-48395]


title: Old_4, Cell 347 of 384 [AS-443076-LR-48395]


title: Old_4, Cell 346 of 384 [AS-443074-LR-48395]


title: Old_4, Cell 345 of 384 [AS-443072-LR-48395]


title: Old_4, Cell 343 of 384 [AS-443068-LR-48395]


title: Old_4, Cell 339 of 384 [AS-443060-LR-48395]


title: Old_4, Cell 344 of 384 [AS-443070-LR-48395]


title: Old_4, Cell 338 of 384 [AS-443058-LR-48395]


title: Old_4, Cell 340 of 384 [AS-443062-LR-48395]


title: Old_4, Cell 341 of 384 [AS-443064-LR-48395]


title: Old_4, Cell 337 of 384 [AS-443056-LR-48395]


title: Old_4, Cell 335 of 384 [AS-443052-LR-48395]


title: Old_4, Cell 336 of 384 [AS-443054-LR-48395]


title: Old_4, Cell 334 of 384 [AS-443050-LR-48395]


title: Old_4, Cell 333 of 384 [AS-443048-LR-48395]


title: Old_4, Cell 342 of 384 [AS-443066-LR-48395]


title: Old_4, Cell 330 of 384 [AS-443042-LR-48395]


title: Old_4, Cell 326 of 384 [AS-443034-LR-48395]


title: Old_4, Cell 332 of 384 [AS-443046-LR-48395]


title: Old_4, Cell 328 of 384 [AS-443038-LR-48395]


title: Old_4, Cell 327 of 384 [AS-443036-LR-48395]


title: Old_4, Cell 331 of 384 [AS-443044-LR-48395]


title: Old_4, Cell 329 of 384 [AS-443040-LR-48395]


title: Old_4, Cell 324 of 384 [AS-443030-LR-48395]


title: Old_4, Cell 319 of 384 [AS-443020-LR-48395]


title: Old_4, Cell 325 of 384 [AS-443032-LR-48395]


title: Old_4, Cell 323 of 384 [AS-443028-LR-48395]


title: Old_4, Cell 322 of 384 [AS-443026-LR-48395]


title: Old_4, Cell 320 of 384 [AS-443022-LR-48395]


title: Old_4, Cell 321 of 384 [AS-443024-LR-48395]


title: Old_4, Cell 315 of 384 [AS-443012-LR-48395]


title: Old_4, Cell 316 of 384 [AS-443014-LR-48395]


title: Old_4, Cell 317 of 384 [AS-443016-LR-48395]


title: Old_4, Cell 318 of 384 [AS-443018-LR-48395]


title: Old_4, Cell 314 of 384 [AS-443010-LR-48395]


title: Old_4, Cell 312 of 384 [AS-443006-LR-48395]


title: Old_4, Cell 313 of 384 [AS-443008-LR-48395]


title: Old_4, Cell 310 of 384 [AS-443002-LR-48395]


title: Old_4, Cell 311 of 384 [AS-443004-LR-48395]


title: Old_4, Cell 309 of 384 [AS-443000-LR-48395]


title: Old_4, Cell 308 of 384 [AS-442998-LR-48395]


title: Old_4, Cell 307 of 384 [AS-442996-LR-48395]


title: Old_4, Cell 306 of 384 [AS-442994-LR-48395]


title: Old_4, Cell 301 of 384 [AS-442984-LR-48395]


title: Old_4, Cell 304 of 384 [AS-442990-LR-48395]


title: Old_4, Cell 299 of 384 [AS-442980-LR-48395]


title: Old_4, Cell 302 of 384 [AS-442986-LR-48395]


title: Old_4, Cell 305 of 384 [AS-442992-LR-48395]


title: Old_4, Cell 300 of 384 [AS-442982-LR-48395]


title: Old_4, Cell 298 of 384 [AS-442978-LR-48395]


title: Old_4, Cell 297 of 384 [AS-442976-LR-48395]


title: Old_4, Cell 295 of 384 [AS-442972-LR-48395]


title: Old_4, Cell 292 of 384 [AS-442966-LR-48395]


title: Old_4, Cell 296 of 384 [AS-442974-LR-48395]


title: Old_4, Cell 294 of 384 [AS-442970-LR-48395]


title: Old_4, Cell 288 of 384 [AS-442958-LR-48395]


title: Old_4, Cell 293 of 384 [AS-442968-LR-48395]


title: Old_4, Cell 287 of 384 [AS-442956-LR-48395]


title: Old_4, Cell 289 of 384 [AS-442960-LR-48395]


title: Old_4, Cell 286 of 384 [AS-442954-LR-48395]


title: Old_4, Cell 290 of 384 [AS-442962-LR-48395]


title: Old_4, Cell 285 of 384 [AS-442952-LR-48395]


title: Old_4, Cell 283 of 384 [AS-442948-LR-48395]


title: Old_4, Cell 284 of 384 [AS-442950-LR-48395]


title: Old_4, Cell 280 of 384 [AS-442942-LR-48395]


title: Old_4, Cell 281 of 384 [AS-442944-LR-48395]


title: Old_4, Cell 279 of 384 [AS-442940-LR-48395]


title: Old_4, Cell 278 of 384 [AS-442938-LR-48395]


title: Old_4, Cell 303 of 384 [AS-442988-LR-48395]


title: Old_4, Cell 277 of 384 [AS-442936-LR-48395]


title: Old_4, Cell 276 of 384 [AS-442934-LR-48395]


title: Old_4, Cell 274 of 384 [AS-442930-LR-48395]


title: Old_4, Cell 272 of 384 [AS-442926-LR-48395]


title: Old_4, Cell 291 of 384 [AS-442964-LR-48395]


title: Old_4, Cell 282 of 384 [AS-442946-LR-48395]


title: Old_4, Cell 271 of 384 [AS-442924-LR-48395]


title: Young_2, Cell 40 of 384 [AS-440908-LR-48372]


title: Old_4, Cell 270 of 384 [AS-442922-LR-48395]


title: Old_4, Cell 266 of 384 [AS-442914-LR-48395]


title: Old_4, Cell 260 of 384 [AS-442902-LR-48395]


title: Old_4, Cell 259 of 384 [AS-442900-LR-48395]


title: Old_4, Cell 264 of 384 [AS-442910-LR-48395]


title: Old_4, Cell 265 of 384 [AS-442912-LR-48395]


title: Old_4, Cell 257 of 384 [AS-442896-LR-48395]


title: Old_4, Cell 263 of 384 [AS-442908-LR-48395]


title: Old_4, Cell 256 of 384 [AS-442894-LR-48395]


title: Old_4, Cell 267 of 384 [AS-442916-LR-48395]


title: Old_4, Cell 255 of 384 [AS-442892-LR-48395]


title: Old_4, Cell 268 of 384 [AS-442918-LR-48395]


title: Old_4, Cell 254 of 384 [AS-442890-LR-48395]


title: Old_4, Cell 258 of 384 [AS-442898-LR-48395]


title: Old_4, Cell 251 of 384 [AS-442884-LR-48395]


title: Old_4, Cell 273 of 384 [AS-442928-LR-48395]


title: Old_4, Cell 250 of 384 [AS-442882-LR-48395]


title: Old_4, Cell 269 of 384 [AS-442920-LR-48395]


title: Old_4, Cell 262 of 384 [AS-442906-LR-48395]


title: Old_4, Cell 261 of 384 [AS-442904-LR-48395]


title: Old_4, Cell 247 of 384 [AS-442876-LR-48395]


title: Old_4, Cell 245 of 384 [AS-442872-LR-48395]


title: Old_4, Cell 246 of 384 [AS-442874-LR-48395]


title: Old_4, Cell 241 of 384 [AS-442864-LR-48395]


title: Old_4, Cell 243 of 384 [AS-442868-LR-48395]


title: Old_4, Cell 242 of 384 [AS-442866-LR-48395]


title: Old_4, Cell 240 of 384 [AS-442862-LR-48395]


title: Old_4, Cell 237 of 384 [AS-442856-LR-48395]


title: Old_4, Cell 253 of 384 [AS-442888-LR-48395]


title: Old_4, Cell 239 of 384 [AS-442860-LR-48395]


title: Old_4, Cell 236 of 384 [AS-442854-LR-48395]


title: Old_4, Cell 238 of 384 [AS-442858-LR-48395]


title: Old_4, Cell 233 of 384 [AS-442848-LR-48395]


title: Old_4, Cell 252 of 384 [AS-442886-LR-48395]


title: Old_4, Cell 235 of 384 [AS-442852-LR-48395]


title: Old_4, Cell 232 of 384 [AS-442846-LR-48395]


title: Old_4, Cell 230 of 384 [AS-442842-LR-48395]


title: Old_4, Cell 231 of 384 [AS-442844-LR-48395]


title: Old_4, Cell 229 of 384 [AS-442840-LR-48395]


title: Old_4, Cell 249 of 384 [AS-442880-LR-48395]


title: Old_4, Cell 228 of 384 [AS-442838-LR-48395]


title: Old_4, Cell 227 of 384 [AS-442836-LR-48395]


title: Old_4, Cell 248 of 384 [AS-442878-LR-48395]


title: Old_4, Cell 226 of 384 [AS-442834-LR-48395]


title: Old_4, Cell 244 of 384 [AS-442870-LR-48395]


title: Old_4, Cell 225 of 384 [AS-442832-LR-48395]


title: Old_4, Cell 224 of 384 [AS-442830-LR-48395]


title: Old_4, Cell 223 of 384 [AS-442828-LR-48395]


title: Old_4, Cell 222 of 384 [AS-442826-LR-48395]


title: Old_4, Cell 221 of 384 [AS-442824-LR-48395]


title: Old_4, Cell 220 of 384 [AS-442822-LR-48395]


title: Old_4, Cell 234 of 384 [AS-442850-LR-48395]


title: Old_4, Cell 218 of 384 [AS-442818-LR-48395]


title: Old_4, Cell 219 of 384 [AS-442820-LR-48395]


title: Old_4, Cell 216 of 384 [AS-442814-LR-48395]


title: Old_4, Cell 217 of 384 [AS-442816-LR-48395]


title: Old_4, Cell 214 of 384 [AS-442810-LR-48395]


title: Old_4, Cell 210 of 384 [AS-442802-LR-48395]


title: Old_4, Cell 211 of 384 [AS-442804-LR-48395]


title: Old_4, Cell 212 of 384 [AS-442806-LR-48395]


title: Old_4, Cell 213 of 384 [AS-442808-LR-48395]


title: Old_4, Cell 209 of 384 [AS-442800-LR-48395]


title: Old_4, Cell 206 of 384 [AS-442794-LR-48395]


title: Old_4, Cell 208 of 384 [AS-442798-LR-48395]


title: Old_4, Cell 204 of 384 [AS-442790-LR-48395]


title: Old_4, Cell 201 of 384 [AS-442784-LR-48395]


title: Old_4, Cell 202 of 384 [AS-442786-LR-48395]


title: Old_4, Cell 203 of 384 [AS-442788-LR-48395]


title: Old_4, Cell 198 of 384 [AS-442778-LR-48395]


title: Old_4, Cell 199 of 384 [AS-442780-LR-48395]


title: Old_4, Cell 196 of 384 [AS-442774-LR-48395]


title: Old_4, Cell 195 of 384 [AS-442772-LR-48395]


title: Old_4, Cell 197 of 384 [AS-442776-LR-48395]


title: Old_4, Cell 194 of 384 [AS-442770-LR-48395]


title: Old_4, Cell 193 of 384 [AS-442768-LR-48395]


title: Old_4, Cell 190 of 384 [AS-442762-LR-48395]


title: Old_4, Cell 207 of 384 [AS-442796-LR-48395]


title: Old_4, Cell 185 of 384 [AS-442752-LR-48395]


title: Old_4, Cell 189 of 384 [AS-442760-LR-48395]


title: Old_4, Cell 183 of 384 [AS-442748-LR-48395]


title: Old_4, Cell 192 of 384 [AS-442766-LR-48395]


title: Old_4, Cell 191 of 384 [AS-442764-LR-48395]


title: Old_4, Cell 181 of 384 [AS-442744-LR-48395]


title: Old_4, Cell 205 of 384 [AS-442792-LR-48395]


title: Old_4, Cell 186 of 384 [AS-442754-LR-48395]


title: Old_4, Cell 187 of 384 [AS-442756-LR-48395]


title: Old_4, Cell 188 of 384 [AS-442758-LR-48395]


title: Old_4, Cell 177 of 384 [AS-442736-LR-48395]


title: Old_4, Cell 176 of 384 [AS-442734-LR-48395]


title: Old_4, Cell 184 of 384 [AS-442750-LR-48395]


title: Old_4, Cell 173 of 384 [AS-442728-LR-48395]


title: Old_4, Cell 171 of 384 [AS-442724-LR-48395]


title: Old_4, Cell 172 of 384 [AS-442726-LR-48395]


title: Old_4, Cell 174 of 384 [AS-442730-LR-48395]


title: Old_4, Cell 275 of 384 [AS-442932-LR-48395]


title: Old_4, Cell 168 of 384 [AS-442718-LR-48395]


title: Old_4, Cell 170 of 384 [AS-442722-LR-48395]


title: Old_4, Cell 169 of 384 [AS-442720-LR-48395]


title: Old_4, Cell 182 of 384 [AS-442746-LR-48395]


title: Old_4, Cell 166 of 384 [AS-442714-LR-48395]


title: Old_4, Cell 163 of 384 [AS-442708-LR-48395]


title: Old_4, Cell 165 of 384 [AS-442712-LR-48395]


title: Old_4, Cell 164 of 384 [AS-442710-LR-48395]


title: Old_4, Cell 161 of 384 [AS-442704-LR-48395]


title: Old_4, Cell 157 of 384 [AS-442696-LR-48395]


title: Old_4, Cell 160 of 384 [AS-442702-LR-48395]


title: Old_4, Cell 180 of 384 [AS-442742-LR-48395]


title: Old_4, Cell 158 of 384 [AS-442698-LR-48395]


title: Old_4, Cell 159 of 384 [AS-442700-LR-48395]


title: Old_4, Cell 155 of 384 [AS-442692-LR-48395]


title: Old_4, Cell 167 of 384 [AS-442716-LR-48395]


title: Old_4, Cell 149 of 384 [AS-442680-LR-48395]


title: Old_4, Cell 151 of 384 [AS-442684-LR-48395]


title: Old_4, Cell 153 of 384 [AS-442688-LR-48395]


title: Old_4, Cell 154 of 384 [AS-442690-LR-48395]


title: Old_4, Cell 162 of 384 [AS-442706-LR-48395]


title: Old_4, Cell 146 of 384 [AS-442674-LR-48395]


title: Old_4, Cell 147 of 384 [AS-442676-LR-48395]


title: Old_4, Cell 143 of 384 [AS-442668-LR-48395]


title: Old_4, Cell 144 of 384 [AS-442670-LR-48395]


title: Old_4, Cell 142 of 384 [AS-442666-LR-48395]


title: Old_4, Cell 156 of 384 [AS-442694-LR-48395]


title: Old_4, Cell 148 of 384 [AS-442678-LR-48395]


title: Old_4, Cell 139 of 384 [AS-442660-LR-48395]


title: Old_4, Cell 150 of 384 [AS-442682-LR-48395]


title: Old_4, Cell 138 of 384 [AS-442658-LR-48395]


title: Old_4, Cell 137 of 384 [AS-442656-LR-48395]


title: Old_4, Cell 152 of 384 [AS-442686-LR-48395]


title: Old_4, Cell 135 of 384 [AS-442652-LR-48395]


title: Old_4, Cell 134 of 384 [AS-442650-LR-48395]


title: Old_4, Cell 128 of 384 [AS-442638-LR-48395]


title: Old_4, Cell 133 of 384 [AS-442648-LR-48395]


title: Old_4, Cell 129 of 384 [AS-442640-LR-48395]


title: Old_4, Cell 127 of 384 [AS-442636-LR-48395]


title: Old_4, Cell 145 of 384 [AS-442672-LR-48395]


title: Old_4, Cell 132 of 384 [AS-442646-LR-48395]


title: Old_4, Cell 130 of 384 [AS-442642-LR-48395]


title: Old_4, Cell 126 of 384 [AS-442634-LR-48395]


title: Old_4, Cell 140 of 384 [AS-442662-LR-48395]


title: Old_4, Cell 215 of 384 [AS-442812-LR-48395]


title: Old_4, Cell 120 of 384 [AS-442622-LR-48395]


title: Old_4, Cell 179 of 384 [AS-442740-LR-48395]


title: Old_4, Cell 125 of 384 [AS-442632-LR-48395]


title: Old_4, Cell 118 of 384 [AS-442618-LR-48395]


title: Old_4, Cell 115 of 384 [AS-442612-LR-48395]


title: Old_4, Cell 136 of 384 [AS-442654-LR-48395]


title: Old_4, Cell 175 of 384 [AS-442732-LR-48395]


title: Old_4, Cell 124 of 384 [AS-442630-LR-48395]


title: Old_4, Cell 123 of 384 [AS-442628-LR-48395]


title: Old_4, Cell 131 of 384 [AS-442644-LR-48395]


title: Old_4, Cell 121 of 384 [AS-442624-LR-48395]


title: Old_4, Cell 114 of 384 [AS-442610-LR-48395]


title: Old_4, Cell 119 of 384 [AS-442620-LR-48395]


title: Old_4, Cell 116 of 384 [AS-442614-LR-48395]


title: Old_4, Cell 113 of 384 [AS-442608-LR-48395]


title: Old_4, Cell 112 of 384 [AS-442606-LR-48395]


title: Old_4, Cell 109 of 384 [AS-442600-LR-48395]


title: Old_4, Cell 107 of 384 [AS-442596-LR-48395]


title: Old_4, Cell 111 of 384 [AS-442604-LR-48395]


title: Old_4, Cell 108 of 384 [AS-442598-LR-48395]


title: Old_4, Cell 122 of 384 [AS-442626-LR-48395]


title: Old_4, Cell 178 of 384 [AS-442738-LR-48395]


title: Old_4, Cell 103 of 384 [AS-442588-LR-48395]


title: Old_4, Cell 104 of 384 [AS-442590-LR-48395]


title: Old_4, Cell 102 of 384 [AS-442586-LR-48395]


title: Old_4, Cell 141 of 384 [AS-442664-LR-48395]


title: Old_4, Cell 117 of 384 [AS-442616-LR-48395]


title: Old_4, Cell 105 of 384 [AS-442592-LR-48395]


title: Old_4, Cell 200 of 384 [AS-442782-LR-48395]


title: Old_4, Cell 100 of 384 [AS-442582-LR-48395]


title: Old_4, Cell 93 of 384 [AS-442568-LR-48395]


title: Old_4, Cell 92 of 384 [AS-442566-LR-48395]


title: Old_4, Cell 97 of 384 [AS-442576-LR-48395]


title: Old_4, Cell 99 of 384 [AS-442580-LR-48395]


title: Old_4, Cell 95 of 384 [AS-442572-LR-48395]


title: Old_4, Cell 98 of 384 [AS-442578-LR-48395]


title: Old_4, Cell 94 of 384 [AS-442570-LR-48395]


title: Old_4, Cell 91 of 384 [AS-442564-LR-48395]


title: Old_4, Cell 96 of 384 [AS-442574-LR-48395]


title: Old_4, Cell 110 of 384 [AS-442602-LR-48395]


title: Old_4, Cell 88 of 384 [AS-442558-LR-48395]


title: Old_4, Cell 90 of 384 [AS-442562-LR-48395]


title: Old_4, Cell 89 of 384 [AS-442560-LR-48395]


title: Old_4, Cell 106 of 384 [AS-442594-LR-48395]


title: Old_4, Cell 86 of 384 [AS-442554-LR-48395]


title: Old_4, Cell 82 of 384 [AS-442546-LR-48395]


title: Old_4, Cell 85 of 384 [AS-442552-LR-48395]


title: Old_4, Cell 83 of 384 [AS-442548-LR-48395]


title: Old_4, Cell 77 of 384 [AS-442536-LR-48395]


title: Old_4, Cell 101 of 384 [AS-442584-LR-48395]


title: Old_4, Cell 84 of 384 [AS-442550-LR-48395]


title: Old_4, Cell 87 of 384 [AS-442556-LR-48395]


title: Old_4, Cell 81 of 384 [AS-442544-LR-48395]


title: Old_4, Cell 78 of 384 [AS-442538-LR-48395]


title: Old_4, Cell 79 of 384 [AS-442540-LR-48395]


title: Old_4, Cell 80 of 384 [AS-442542-LR-48395]


title: Old_4, Cell 75 of 384 [AS-442532-LR-48395]


title: Old_4, Cell 76 of 384 [AS-442534-LR-48395]


title: Old_4, Cell 74 of 384 [AS-442530-LR-48395]


title: Old_4, Cell 71 of 384 [AS-442524-LR-48395]


title: Old_4, Cell 72 of 384 [AS-442526-LR-48395]


title: Old_4, Cell 69 of 384 [AS-442520-LR-48395]


title: Old_4, Cell 66 of 384 [AS-442514-LR-48395]


title: Old_4, Cell 67 of 384 [AS-442516-LR-48395]


title: Old_4, Cell 70 of 384 [AS-442522-LR-48395]


title: Old_4, Cell 73 of 384 [AS-442528-LR-48395]


title: Old_4, Cell 64 of 384 [AS-442510-LR-48395]


title: Old_4, Cell 62 of 384 [AS-442506-LR-48395]


title: Old_4, Cell 61 of 384 [AS-442504-LR-48395]


title: Old_4, Cell 68 of 384 [AS-442518-LR-48395]


title: Old_4, Cell 65 of 384 [AS-442512-LR-48395]


title: Old_4, Cell 63 of 384 [AS-442508-LR-48395]


title: Old_4, Cell 57 of 384 [AS-442496-LR-48395]


title: Old_4, Cell 59 of 384 [AS-442500-LR-48395]


title: Old_4, Cell 55 of 384 [AS-442492-LR-48395]


title: Old_4, Cell 58 of 384 [AS-442498-LR-48395]


title: Old_4, Cell 56 of 384 [AS-442494-LR-48395]


title: Old_4, Cell 48 of 384 [AS-442478-LR-48395]


title: Old_4, Cell 49 of 384 [AS-442480-LR-48395]


title: Old_4, Cell 60 of 384 [AS-442502-LR-48395]


title: Old_4, Cell 51 of 384 [AS-442484-LR-48395]


title: Old_4, Cell 54 of 384 [AS-442490-LR-48395]


title: Old_4, Cell 50 of 384 [AS-442482-LR-48395]


title: Old_4, Cell 44 of 384 [AS-442470-LR-48395]


title: Old_4, Cell 45 of 384 [AS-442472-LR-48395]


title: Old_4, Cell 52 of 384 [AS-442486-LR-48395]


title: Old_4, Cell 43 of 384 [AS-442468-LR-48395]


title: Old_4, Cell 41 of 384 [AS-442464-LR-48395]


title: Old_4, Cell 42 of 384 [AS-442466-LR-48395]


title: Old_4, Cell 40 of 384 [AS-442462-LR-48395]


title: Old_4, Cell 38 of 384 [AS-442458-LR-48395]


title: Old_4, Cell 36 of 384 [AS-442454-LR-48395]


title: Old_4, Cell 53 of 384 [AS-442488-LR-48395]


title: Old_4, Cell 35 of 384 [AS-442452-LR-48395]


title: Old_4, Cell 34 of 384 [AS-442450-LR-48395]


title: Old_4, Cell 37 of 384 [AS-442456-LR-48395]


title: Old_4, Cell 46 of 384 [AS-442474-LR-48395]


title: Old_4, Cell 39 of 384 [AS-442460-LR-48395]


title: Old_4, Cell 32 of 384 [AS-442446-LR-48395]


title: Old_4, Cell 33 of 384 [AS-442448-LR-48395]


title: Old_4, Cell 28 of 384 [AS-442438-LR-48395]


title: Old_4, Cell 29 of 384 [AS-442440-LR-48395]


title: Old_4, Cell 30 of 384 [AS-442442-LR-48395]


title: Old_4, Cell 47 of 384 [AS-442476-LR-48395]


title: Old_4, Cell 31 of 384 [AS-442444-LR-48395]


title: Old_4, Cell 27 of 384 [AS-442436-LR-48395]


title: Old_4, Cell 26 of 384 [AS-442434-LR-48395]


title: Old_4, Cell 22 of 384 [AS-442426-LR-48395]


title: Old_4, Cell 25 of 384 [AS-442432-LR-48395]


title: Old_4, Cell 20 of 384 [AS-442422-LR-48395]


title: Old_4, Cell 24 of 384 [AS-442430-LR-48395]


title: Old_4, Cell 23 of 384 [AS-442428-LR-48395]


title: Old_4, Cell 19 of 384 [AS-442420-LR-48395]


title: Old_4, Cell 16 of 384 [AS-442414-LR-48395]


title: Old_4, Cell 18 of 384 [AS-442418-LR-48395]


title: Old_4, Cell 21 of 384 [AS-442424-LR-48395]


title: Old_4, Cell 17 of 384 [AS-442416-LR-48395]


title: Old_4, Cell 15 of 384 [AS-442412-LR-48395]


title: Old_4, Cell 12 of 384 [AS-442406-LR-48395]


title: Old_4, Cell 9 of 384 [AS-442400-LR-48395]


title: Old_4, Cell 8 of 384 [AS-442398-LR-48395]


title: Old_4, Cell 10 of 384 [AS-442402-LR-48395]


title: Old_4, Cell 6 of 384 [AS-442394-LR-48395]


title: Old_4, Cell 13 of 384 [AS-442408-LR-48395]


title: Old_4, Cell 4 of 384 [AS-442390-LR-48395]


title: Old_4, Cell 3 of 384 [AS-442388-LR-48395]


title: Old_4, Cell 7 of 384 [AS-442396-LR-48395]


title: Old_4, Cell 14 of 384 [AS-442410-LR-48395]


title: Old_4, Cell 1 of 384 [AS-442384-LR-48395]


title: Old_4, Cell 5 of 384 [AS-442392-LR-48395]


title: Old_4, Cell 2 of 384 [AS-442386-LR-48395]


title: Old_3, Cell 192 of 192 [AS-440827-LR-48373]


title: Old_3, Cell 190 of 192 [AS-440823-LR-48373]


title: Old_3, Cell 191 of 192 [AS-440825-LR-48373]


title: Old_4, Cell 11 of 384 [AS-442404-LR-48395]


title: Old_3, Cell 188 of 192 [AS-440819-LR-48373]


title: Old_3, Cell 184 of 192 [AS-440811-LR-48373]


title: Old_3, Cell 189 of 192 [AS-440821-LR-48373]


title: Old_3, Cell 185 of 192 [AS-440813-LR-48373]


title: Old_3, Cell 186 of 192 [AS-440815-LR-48373]


title: Old_3, Cell 183 of 192 [AS-440809-LR-48373]


title: Old_3, Cell 182 of 192 [AS-440807-LR-48373]


title: Old_3, Cell 181 of 192 [AS-440805-LR-48373]


title: Old_3, Cell 177 of 192 [AS-440797-LR-48373]


title: Old_3, Cell 178 of 192 [AS-440799-LR-48373]


title: Old_3, Cell 180 of 192 [AS-440803-LR-48373]


title: Old_3, Cell 176 of 192 [AS-440795-LR-48373]


title: Old_3, Cell 179 of 192 [AS-440801-LR-48373]


title: Old_3, Cell 173 of 192 [AS-440789-LR-48373]


title: Old_3, Cell 175 of 192 [AS-440793-LR-48373]


title: Old_3, Cell 171 of 192 [AS-440785-LR-48373]


title: Old_3, Cell 174 of 192 [AS-440791-LR-48373]


title: Old_3, Cell 169 of 192 [AS-440781-LR-48373]


title: Old_3, Cell 170 of 192 [AS-440783-LR-48373]


title: Old_3, Cell 172 of 192 [AS-440787-LR-48373]


title: Old_3, Cell 165 of 192 [AS-440773-LR-48373]


title: Old_3, Cell 167 of 192 [AS-440777-LR-48373]


title: Old_3, Cell 166 of 192 [AS-440775-LR-48373]


title: Old_3, Cell 187 of 192 [AS-440817-LR-48373]


title: Old_3, Cell 163 of 192 [AS-440769-LR-48373]


title: Old_3, Cell 164 of 192 [AS-440771-LR-48373]


title: Old_3, Cell 162 of 192 [AS-440767-LR-48373]


title: Old_3, Cell 160 of 192 [AS-440763-LR-48373]


title: Old_3, Cell 161 of 192 [AS-440765-LR-48373]


title: Old_3, Cell 156 of 192 [AS-440755-LR-48373]


title: Old_3, Cell 158 of 192 [AS-440759-LR-48373]


title: Old_3, Cell 154 of 192 [AS-440751-LR-48373]


title: Old_3, Cell 155 of 192 [AS-440753-LR-48373]


title: Old_3, Cell 153 of 192 [AS-440749-LR-48373]


title: Old_3, Cell 152 of 192 [AS-440747-LR-48373]


title: Old_3, Cell 148 of 192 [AS-440739-LR-48373]


title: Old_3, Cell 168 of 192 [AS-440779-LR-48373]


title: Old_3, Cell 149 of 192 [AS-440741-LR-48373]


title: Old_3, Cell 146 of 192 [AS-440735-LR-48373]


title: Old_3, Cell 147 of 192 [AS-440737-LR-48373]


title: Old_3, Cell 145 of 192 [AS-440733-LR-48373]


title: Old_3, Cell 143 of 192 [AS-440729-LR-48373]


title: Old_3, Cell 144 of 192 [AS-440731-LR-48373]


title: Old_3, Cell 159 of 192 [AS-440761-LR-48373]


title: Old_3, Cell 136 of 192 [AS-440715-LR-48373]


title: Old_3, Cell 139 of 192 [AS-440721-LR-48373]


title: Old_3, Cell 142 of 192 [AS-440727-LR-48373]


title: Old_3, Cell 135 of 192 [AS-440713-LR-48373]


title: Old_3, Cell 157 of 192 [AS-440757-LR-48373]


title: Old_3, Cell 134 of 192 [AS-440711-LR-48373]


title: Old_3, Cell 138 of 192 [AS-440719-LR-48373]


title: Old_3, Cell 133 of 192 [AS-440709-LR-48373]


title: Old_3, Cell 132 of 192 [AS-440707-LR-48373]


title: Old_3, Cell 131 of 192 [AS-440705-LR-48373]


title: Old_3, Cell 127 of 192 [AS-440697-LR-48373]


title: Old_3, Cell 130 of 192 [AS-440703-LR-48373]


title: Old_3, Cell 150 of 192 [AS-440743-LR-48373]


title: Old_3, Cell 151 of 192 [AS-440745-LR-48373]


title: Old_3, Cell 141 of 192 [AS-440725-LR-48373]


title: Old_3, Cell 125 of 192 [AS-440693-LR-48373]


title: Old_3, Cell 123 of 192 [AS-440689-LR-48373]


title: Old_3, Cell 124 of 192 [AS-440691-LR-48373]


title: Old_3, Cell 126 of 192 [AS-440695-LR-48373]


title: Old_3, Cell 140 of 192 [AS-440723-LR-48373]


title: Old_3, Cell 121 of 192 [AS-440685-LR-48373]


title: Old_3, Cell 120 of 192 [AS-440683-LR-48373]


title: Old_3, Cell 122 of 192 [AS-440687-LR-48373]


title: Old_3, Cell 137 of 192 [AS-440717-LR-48373]


title: Old_3, Cell 119 of 192 [AS-440681-LR-48373]


title: Old_3, Cell 117 of 192 [AS-440677-LR-48373]


title: Old_3, Cell 112 of 192 [AS-440667-LR-48373]


title: Old_3, Cell 111 of 192 [AS-440665-LR-48373]


title: Old_3, Cell 110 of 192 [AS-440663-LR-48373]


title: Old_3, Cell 115 of 192 [AS-440673-LR-48373]


title: Old_3, Cell 114 of 192 [AS-440671-LR-48373]


title: Old_3, Cell 129 of 192 [AS-440701-LR-48373]


title: Old_3, Cell 116 of 192 [AS-440675-LR-48373]


title: Old_3, Cell 128 of 192 [AS-440699-LR-48373]


title: Old_3, Cell 107 of 192 [AS-440657-LR-48373]


title: Old_3, Cell 113 of 192 [AS-440669-LR-48373]


title: Old_3, Cell 105 of 192 [AS-440653-LR-48373]


title: Old_3, Cell 108 of 192 [AS-440659-LR-48373]


title: Old_3, Cell 106 of 192 [AS-440655-LR-48373]


title: Old_3, Cell 104 of 192 [AS-440651-LR-48373]


title: Old_3, Cell 102 of 192 [AS-440647-LR-48373]


title: Old_3, Cell 103 of 192 [AS-440649-LR-48373]


title: Old_3, Cell 94 of 192 [AS-440631-LR-48373]


title: Old_3, Cell 93 of 192 [AS-440629-LR-48373]


title: Old_3, Cell 109 of 192 [AS-440661-LR-48373]


title: Old_3, Cell 92 of 192 [AS-440627-LR-48373]


title: Old_3, Cell 101 of 192 [AS-440645-LR-48373]


title: Old_3, Cell 99 of 192 [AS-440641-LR-48373]


title: Old_3, Cell 96 of 192 [AS-440635-LR-48373]


title: Old_3, Cell 100 of 192 [AS-440643-LR-48373]


title: Old_3, Cell 95 of 192 [AS-440633-LR-48373]


title: Old_3, Cell 118 of 192 [AS-440679-LR-48373]


title: Old_3, Cell 90 of 192 [AS-440623-LR-48373]


title: Old_3, Cell 98 of 192 [AS-440639-LR-48373]


title: Old_3, Cell 88 of 192 [AS-440619-LR-48373]


title: Old_3, Cell 89 of 192 [AS-440621-LR-48373]


title: Old_3, Cell 97 of 192 [AS-440637-LR-48373]


title: Old_3, Cell 81 of 192 [AS-440605-LR-48373]


title: Old_3, Cell 79 of 192 [AS-440601-LR-48373]


title: Old_3, Cell 83 of 192 [AS-440609-LR-48373]


title: Old_3, Cell 85 of 192 [AS-440613-LR-48373]


title: Old_3, Cell 82 of 192 [AS-440607-LR-48373]


title: Old_3, Cell 86 of 192 [AS-440615-LR-48373]


title: Old_3, Cell 84 of 192 [AS-440611-LR-48373]


title: Old_3, Cell 87 of 192 [AS-440617-LR-48373]


title: Old_3, Cell 78 of 192 [AS-440599-LR-48373]


title: Old_3, Cell 77 of 192 [AS-440597-LR-48373]


title: Old_3, Cell 75 of 192 [AS-440593-LR-48373]


title: Old_3, Cell 76 of 192 [AS-440595-LR-48373]


title: Old_3, Cell 74 of 192 [AS-440591-LR-48373]


title: Old_3, Cell 91 of 192 [AS-440625-LR-48373]


title: Old_3, Cell 71 of 192 [AS-440585-LR-48373]


title: Old_3, Cell 73 of 192 [AS-440589-LR-48373]


title: Old_3, Cell 72 of 192 [AS-440587-LR-48373]


title: Old_3, Cell 70 of 192 [AS-440583-LR-48373]


title: Old_3, Cell 69 of 192 [AS-440581-LR-48373]


title: Old_3, Cell 68 of 192 [AS-440579-LR-48373]


title: Old_3, Cell 67 of 192 [AS-440577-LR-48373]


title: Old_3, Cell 66 of 192 [AS-440575-LR-48373]


title: Old_3, Cell 80 of 192 [AS-440603-LR-48373]


title: Old_3, Cell 65 of 192 [AS-440573-LR-48373]


title: Old_3, Cell 64 of 192 [AS-440571-LR-48373]


title: Old_3, Cell 60 of 192 [AS-440563-LR-48373]


title: Old_3, Cell 61 of 192 [AS-440565-LR-48373]


title: Old_3, Cell 49 of 192 [AS-440541-LR-48373]


title: Old_3, Cell 51 of 192 [AS-440545-LR-48373]


title: Old_3, Cell 59 of 192 [AS-440561-LR-48373]


title: Old_3, Cell 46 of 192 [AS-440535-LR-48373]


title: Old_3, Cell 55 of 192 [AS-440553-LR-48373]


title: Old_3, Cell 52 of 192 [AS-440547-LR-48373]


title: Old_3, Cell 47 of 192 [AS-440537-LR-48373]


title: Old_3, Cell 56 of 192 [AS-440555-LR-48373]


title: Old_3, Cell 54 of 192 [AS-440551-LR-48373]


title: Old_3, Cell 48 of 192 [AS-440539-LR-48373]


title: Old_3, Cell 57 of 192 [AS-440557-LR-48373]


title: Old_3, Cell 50 of 192 [AS-440543-LR-48373]


title: Old_3, Cell 53 of 192 [AS-440549-LR-48373]


title: Old_3, Cell 45 of 192 [AS-440533-LR-48373]


title: Old_3, Cell 44 of 192 [AS-440531-LR-48373]


title: Old_3, Cell 43 of 192 [AS-440529-LR-48373]


title: Old_3, Cell 63 of 192 [AS-440569-LR-48373]


title: Old_3, Cell 39 of 192 [AS-440521-LR-48373]


title: Old_3, Cell 38 of 192 [AS-440519-LR-48373]


title: Old_3, Cell 42 of 192 [AS-440527-LR-48373]


title: Old_3, Cell 41 of 192 [AS-440525-LR-48373]


title: Old_3, Cell 40 of 192 [AS-440523-LR-48373]


title: Old_3, Cell 35 of 192 [AS-440513-LR-48373]


title: Old_3, Cell 62 of 192 [AS-440567-LR-48373]


title: Old_3, Cell 32 of 192 [AS-440507-LR-48373]


title: Old_3, Cell 36 of 192 [AS-440515-LR-48373]


title: Old_3, Cell 58 of 192 [AS-440559-LR-48373]


title: Old_3, Cell 33 of 192 [AS-440509-LR-48373]


title: Old_3, Cell 34 of 192 [AS-440511-LR-48373]


title: Old_3, Cell 29 of 192 [AS-440501-LR-48373]


title: Old_3, Cell 23 of 192 [AS-440489-LR-48373]


title: Old_3, Cell 22 of 192 [AS-440487-LR-48373]


title: Old_3, Cell 25 of 192 [AS-440493-LR-48373]


title: Old_3, Cell 31 of 192 [AS-440505-LR-48373]


title: Old_3, Cell 24 of 192 [AS-440491-LR-48373]


title: Old_3, Cell 26 of 192 [AS-440495-LR-48373]


title: Old_3, Cell 28 of 192 [AS-440499-LR-48373]


title: Old_3, Cell 37 of 192 [AS-440517-LR-48373]


title: Old_3, Cell 19 of 192 [AS-440481-LR-48373]


title: Old_3, Cell 20 of 192 [AS-440483-LR-48373]


title: Old_3, Cell 18 of 192 [AS-440479-LR-48373]


title: Old_3, Cell 16 of 192 [AS-440475-LR-48373]


title: Old_3, Cell 17 of 192 [AS-440477-LR-48373]


title: Old_3, Cell 15 of 192 [AS-440473-LR-48373]


title: Old_3, Cell 12 of 192 [AS-440467-LR-48373]


title: Old_3, Cell 11 of 192 [AS-440465-LR-48373]


title: Old_3, Cell 10 of 192 [AS-440463-LR-48373]


title: Old_3, Cell 14 of 192 [AS-440471-LR-48373]


title: Old_3, Cell 9 of 192 [AS-440461-LR-48373]


title: Old_3, Cell 13 of 192 [AS-440469-LR-48373]


title: Old_3, Cell 8 of 192 [AS-440459-LR-48373]


title: Old_3, Cell 6 of 192 [AS-440455-LR-48373]


title: Old_3, Cell 5 of 192 [AS-440453-LR-48373]


title: Old_3, Cell 7 of 192 [AS-440457-LR-48373]


title: Old_3, Cell 30 of 192 [AS-440503-LR-48373]


title: Old_3, Cell 27 of 192 [AS-440497-LR-48373]


title: Old_3, Cell 3 of 192 [AS-440449-LR-48373]


title: Old_3, Cell 21 of 192 [AS-440485-LR-48373]


title: Old_2, Cell 190 of 192 [AS-442361-LR-48394]


title: Old_3, Cell 1 of 192 [AS-440445-LR-48373]


title: Old_2, Cell 187 of 192 [AS-442355-LR-48394]


title: Old_2, Cell 189 of 192 [AS-442359-LR-48394]


title: Old_2, Cell 184 of 192 [AS-442349-LR-48394]


title: Old_2, Cell 186 of 192 [AS-442353-LR-48394]


title: Old_2, Cell 188 of 192 [AS-442357-LR-48394]


title: Old_2, Cell 183 of 192 [AS-442347-LR-48394]


title: Old_2, Cell 181 of 192 [AS-442343-LR-48394]


title: Old_2, Cell 180 of 192 [AS-442341-LR-48394]


title: Old_2, Cell 178 of 192 [AS-442337-LR-48394]


title: Old_2, Cell 192 of 192 [AS-442365-LR-48394]


title: Old_3, Cell 4 of 192 [AS-440451-LR-48373]


title: Old_2, Cell 174 of 192 [AS-442329-LR-48394]


title: Old_2, Cell 177 of 192 [AS-442335-LR-48394]


title: Old_2, Cell 176 of 192 [AS-442333-LR-48394]


title: Old_2, Cell 175 of 192 [AS-442331-LR-48394]


title: Old_2, Cell 172 of 192 [AS-442325-LR-48394]


title: Old_3, Cell 2 of 192 [AS-440447-LR-48373]


title: Old_2, Cell 185 of 192 [AS-442351-LR-48394]


title: Old_2, Cell 170 of 192 [AS-442321-LR-48394]


title: Old_2, Cell 169 of 192 [AS-442319-LR-48394]


title: Old_2, Cell 167 of 192 [AS-442315-LR-48394]


title: Old_2, Cell 168 of 192 [AS-442317-LR-48394]


title: Old_2, Cell 171 of 192 [AS-442323-LR-48394]


title: Old_2, Cell 191 of 192 [AS-442363-LR-48394]


title: Old_2, Cell 166 of 192 [AS-442313-LR-48394]


title: Old_2, Cell 165 of 192 [AS-442311-LR-48394]


title: Old_2, Cell 164 of 192 [AS-442309-LR-48394]


title: Old_2, Cell 182 of 192 [AS-442345-LR-48394]


title: Old_2, Cell 162 of 192 [AS-442305-LR-48394]


title: Old_2, Cell 179 of 192 [AS-442339-LR-48394]


title: Old_2, Cell 163 of 192 [AS-442307-LR-48394]


title: Old_2, Cell 159 of 192 [AS-442299-LR-48394]


title: Old_2, Cell 173 of 192 [AS-442327-LR-48394]


title: Old_2, Cell 158 of 192 [AS-442297-LR-48394]


title: Old_2, Cell 160 of 192 [AS-442301-LR-48394]


title: Old_2, Cell 155 of 192 [AS-442291-LR-48394]


title: Old_2, Cell 157 of 192 [AS-442295-LR-48394]


title: Old_2, Cell 154 of 192 [AS-442289-LR-48394]


title: Old_2, Cell 152 of 192 [AS-442285-LR-48394]


title: Old_2, Cell 156 of 192 [AS-442293-LR-48394]


title: Old_2, Cell 151 of 192 [AS-442283-LR-48394]


title: Old_2, Cell 153 of 192 [AS-442287-LR-48394]


title: Old_2, Cell 145 of 192 [AS-442271-LR-48394]


title: Old_2, Cell 143 of 192 [AS-442267-LR-48394]


title: Old_2, Cell 148 of 192 [AS-442277-LR-48394]


title: Old_2, Cell 150 of 192 [AS-442281-LR-48394]


title: Old_2, Cell 141 of 192 [AS-442263-LR-48394]


title: Old_2, Cell 144 of 192 [AS-442269-LR-48394]


title: Old_2, Cell 147 of 192 [AS-442275-LR-48394]


title: Old_2, Cell 146 of 192 [AS-442273-LR-48394]


title: Old_2, Cell 140 of 192 [AS-442261-LR-48394]


title: Old_2, Cell 161 of 192 [AS-442303-LR-48394]


title: Old_2, Cell 139 of 192 [AS-442259-LR-48394]


title: Old_2, Cell 142 of 192 [AS-442265-LR-48394]


title: Old_2, Cell 135 of 192 [AS-442251-LR-48394]


title: Old_2, Cell 137 of 192 [AS-442255-LR-48394]


title: Old_2, Cell 136 of 192 [AS-442253-LR-48394]


title: Old_2, Cell 138 of 192 [AS-442257-LR-48394]


title: Old_2, Cell 133 of 192 [AS-442247-LR-48394]


title: Old_2, Cell 134 of 192 [AS-442249-LR-48394]


title: Old_2, Cell 129 of 192 [AS-442239-LR-48394]


title: Old_2, Cell 149 of 192 [AS-442279-LR-48394]


title: Old_2, Cell 132 of 192 [AS-442245-LR-48394]


title: Old_2, Cell 131 of 192 [AS-442243-LR-48394]


title: Old_2, Cell 130 of 192 [AS-442241-LR-48394]


title: Old_2, Cell 128 of 192 [AS-442237-LR-48394]


title: Old_2, Cell 127 of 192 [AS-442235-LR-48394]


title: Old_2, Cell 126 of 192 [AS-442233-LR-48394]


title: Old_2, Cell 124 of 192 [AS-442229-LR-48394]


title: Old_2, Cell 123 of 192 [AS-442227-LR-48394]


title: Old_2, Cell 125 of 192 [AS-442231-LR-48394]


title: Old_2, Cell 122 of 192 [AS-442225-LR-48394]


title: Old_2, Cell 120 of 192 [AS-442221-LR-48394]


title: Old_2, Cell 118 of 192 [AS-442217-LR-48394]


title: Old_2, Cell 121 of 192 [AS-442223-LR-48394]


title: Old_2, Cell 116 of 192 [AS-442213-LR-48394]


title: Old_2, Cell 119 of 192 [AS-442219-LR-48394]


title: Old_2, Cell 114 of 192 [AS-442209-LR-48394]


title: Old_2, Cell 115 of 192 [AS-442211-LR-48394]


title: Old_2, Cell 117 of 192 [AS-442215-LR-48394]


title: Old_2, Cell 113 of 192 [AS-442207-LR-48394]


title: Old_2, Cell 112 of 192 [AS-442205-LR-48394]


title: Old_2, Cell 108 of 192 [AS-442197-LR-48394]


title: Old_2, Cell 105 of 192 [AS-442191-LR-48394]


title: Old_2, Cell 109 of 192 [AS-442199-LR-48394]


title: Old_2, Cell 110 of 192 [AS-442201-LR-48394]


title: Old_2, Cell 107 of 192 [AS-442195-LR-48394]


title: Old_2, Cell 104 of 192 [AS-442189-LR-48394]


title: Old_2, Cell 102 of 192 [AS-442185-LR-48394]


title: Old_2, Cell 101 of 192 [AS-442183-LR-48394]


title: Old_2, Cell 100 of 192 [AS-442181-LR-48394]


title: Old_2, Cell 103 of 192 [AS-442187-LR-48394]


title: Old_2, Cell 99 of 192 [AS-442179-LR-48394]


title: Old_2, Cell 97 of 192 [AS-442175-LR-48394]


title: Old_2, Cell 98 of 192 [AS-442177-LR-48394]


title: Old_2, Cell 94 of 192 [AS-442169-LR-48394]


title: Old_2, Cell 92 of 192 [AS-442165-LR-48394]


title: Old_2, Cell 96 of 192 [AS-442173-LR-48394]


title: Old_2, Cell 93 of 192 [AS-442167-LR-48394]


title: Old_2, Cell 95 of 192 [AS-442171-LR-48394]


title: Old_2, Cell 90 of 192 [AS-442161-LR-48394]


title: Old_2, Cell 91 of 192 [AS-442163-LR-48394]


title: Old_2, Cell 88 of 192 [AS-442157-LR-48394]


title: Old_2, Cell 89 of 192 [AS-442159-LR-48394]


title: Old_2, Cell 86 of 192 [AS-442153-LR-48394]


title: Old_2, Cell 87 of 192 [AS-442155-LR-48394]


title: Old_2, Cell 84 of 192 [AS-442149-LR-48394]


title: Old_2, Cell 83 of 192 [AS-442147-LR-48394]


title: Old_2, Cell 85 of 192 [AS-442151-LR-48394]


title: Old_2, Cell 106 of 192 [AS-442193-LR-48394]


title: Old_2, Cell 81 of 192 [AS-442143-LR-48394]


title: Old_2, Cell 82 of 192 [AS-442145-LR-48394]


title: Old_2, Cell 80 of 192 [AS-442141-LR-48394]


title: Old_2, Cell 77 of 192 [AS-442135-LR-48394]


title: Old_2, Cell 78 of 192 [AS-442137-LR-48394]


title: Old_2, Cell 79 of 192 [AS-442139-LR-48394]


title: Old_2, Cell 76 of 192 [AS-442133-LR-48394]


title: Old_2, Cell 74 of 192 [AS-442129-LR-48394]


title: Old_2, Cell 73 of 192 [AS-442127-LR-48394]


title: Old_2, Cell 111 of 192 [AS-442203-LR-48394]


title: Old_2, Cell 72 of 192 [AS-442125-LR-48394]


title: Old_2, Cell 75 of 192 [AS-442131-LR-48394]


title: Old_2, Cell 71 of 192 [AS-442123-LR-48394]


title: Old_2, Cell 70 of 192 [AS-442121-LR-48394]


title: Old_2, Cell 68 of 192 [AS-442117-LR-48394]


title: Old_2, Cell 65 of 192 [AS-442111-LR-48394]


title: Old_2, Cell 69 of 192 [AS-442119-LR-48394]


title: Old_2, Cell 64 of 192 [AS-442109-LR-48394]


title: Old_2, Cell 63 of 192 [AS-442107-LR-48394]


title: Old_2, Cell 66 of 192 [AS-442113-LR-48394]


title: Old_2, Cell 67 of 192 [AS-442115-LR-48394]


title: Old_2, Cell 62 of 192 [AS-442105-LR-48394]


title: Old_2, Cell 60 of 192 [AS-442101-LR-48394]


title: Old_2, Cell 61 of 192 [AS-442103-LR-48394]


title: Old_2, Cell 59 of 192 [AS-442099-LR-48394]


title: Old_2, Cell 56 of 192 [AS-442093-LR-48394]


title: Old_2, Cell 57 of 192 [AS-442095-LR-48394]


title: Old_2, Cell 58 of 192 [AS-442097-LR-48394]


title: Old_2, Cell 55 of 192 [AS-442091-LR-48394]


title: Old_2, Cell 52 of 192 [AS-442085-LR-48394]


title: Old_2, Cell 54 of 192 [AS-442089-LR-48394]


title: Old_2, Cell 51 of 192 [AS-442083-LR-48394]


title: Old_2, Cell 53 of 192 [AS-442087-LR-48394]


title: Old_2, Cell 49 of 192 [AS-442079-LR-48394]


title: Old_2, Cell 47 of 192 [AS-442075-LR-48394]


title: Old_2, Cell 50 of 192 [AS-442081-LR-48394]


title: Old_2, Cell 48 of 192 [AS-442077-LR-48394]


title: Old_2, Cell 45 of 192 [AS-442071-LR-48394]


title: Old_2, Cell 46 of 192 [AS-442073-LR-48394]


title: Old_2, Cell 42 of 192 [AS-442065-LR-48394]


title: Old_2, Cell 43 of 192 [AS-442067-LR-48394]


title: Old_2, Cell 44 of 192 [AS-442069-LR-48394]


title: Old_2, Cell 41 of 192 [AS-442063-LR-48394]


title: Old_2, Cell 36 of 192 [AS-442053-LR-48394]


title: Old_2, Cell 39 of 192 [AS-442059-LR-48394]


title: Old_2, Cell 38 of 192 [AS-442057-LR-48394]


title: Old_2, Cell 40 of 192 [AS-442061-LR-48394]


title: Old_2, Cell 34 of 192 [AS-442049-LR-48394]


title: Old_2, Cell 37 of 192 [AS-442055-LR-48394]


title: Old_2, Cell 30 of 192 [AS-442041-LR-48394]


title: Old_2, Cell 33 of 192 [AS-442047-LR-48394]


title: Old_2, Cell 27 of 192 [AS-442035-LR-48394]


title: Old_2, Cell 26 of 192 [AS-442033-LR-48394]


title: Old_2, Cell 32 of 192 [AS-442045-LR-48394]


title: Old_2, Cell 28 of 192 [AS-442037-LR-48394]


title: Old_2, Cell 23 of 192 [AS-442027-LR-48394]


title: Old_2, Cell 25 of 192 [AS-442031-LR-48394]


title: Old_2, Cell 24 of 192 [AS-442029-LR-48394]


title: Old_2, Cell 21 of 192 [AS-442023-LR-48394]


title: Old_2, Cell 19 of 192 [AS-442019-LR-48394]


title: Old_2, Cell 20 of 192 [AS-442021-LR-48394]


title: Old_2, Cell 18 of 192 [AS-442017-LR-48394]


title: Old_2, Cell 15 of 192 [AS-442011-LR-48394]


title: Old_2, Cell 14 of 192 [AS-442009-LR-48394]


title: Old_2, Cell 31 of 192 [AS-442043-LR-48394]


title: Old_2, Cell 35 of 192 [AS-442051-LR-48394]


title: Old_2, Cell 16 of 192 [AS-442013-LR-48394]


title: Old_2, Cell 13 of 192 [AS-442007-LR-48394]


title: Old_2, Cell 10 of 192 [AS-442001-LR-48394]


title: Old_2, Cell 11 of 192 [AS-442003-LR-48394]


title: Old_2, Cell 12 of 192 [AS-442005-LR-48394]


title: Old_2, Cell 29 of 192 [AS-442039-LR-48394]


title: Old_2, Cell 7 of 192 [AS-441995-LR-48394]


title: Old_2, Cell 22 of 192 [AS-442025-LR-48394]


title: Old_2, Cell 3 of 192 [AS-441987-LR-48394]


title: Old_2, Cell 2 of 192 [AS-441985-LR-48394]


title: Old_1, Cell 190 of 192 [AS-441592-LR-48372]


title: Old_1, Cell 191 of 192 [AS-441594-LR-48372]


title: Old_2, Cell 4 of 192 [AS-441989-LR-48394]


title: Old_1, Cell 192 of 192 [AS-441596-LR-48372]


title: Old_2, Cell 17 of 192 [AS-442015-LR-48394]


title: Old_2, Cell 1 of 192 [AS-441983-LR-48394]


title: Old_1, Cell 188 of 192 [AS-441588-LR-48372]


title: Old_1, Cell 186 of 192 [AS-441584-LR-48372]


title: Old_1, Cell 189 of 192 [AS-441590-LR-48372]


title: Old_2, Cell 6 of 192 [AS-441993-LR-48394]


title: Old_1, Cell 182 of 192 [AS-441576-LR-48372]


title: Old_1, Cell 184 of 192 [AS-441580-LR-48372]


title: Old_2, Cell 8 of 192 [AS-441997-LR-48394]


title: Old_2, Cell 5 of 192 [AS-441991-LR-48394]


title: Old_1, Cell 185 of 192 [AS-441582-LR-48372]


title: Old_1, Cell 179 of 192 [AS-441570-LR-48372]


title: Old_1, Cell 178 of 192 [AS-441568-LR-48372]


title: Old_1, Cell 177 of 192 [AS-441566-LR-48372]


title: Old_1, Cell 175 of 192 [AS-441562-LR-48372]


title: Old_1, Cell 174 of 192 [AS-441560-LR-48372]


title: Old_2, Cell 9 of 192 [AS-441999-LR-48394]


title: Old_1, Cell 181 of 192 [AS-441574-LR-48372]


title: Old_1, Cell 176 of 192 [AS-441564-LR-48372]


title: Old_1, Cell 172 of 192 [AS-441556-LR-48372]


title: Old_1, Cell 180 of 192 [AS-441572-LR-48372]


title: Old_1, Cell 187 of 192 [AS-441586-LR-48372]


title: Old_1, Cell 169 of 192 [AS-441550-LR-48372]


title: Old_1, Cell 171 of 192 [AS-441554-LR-48372]


title: Old_1, Cell 170 of 192 [AS-441552-LR-48372]


title: Old_1, Cell 168 of 192 [AS-441548-LR-48372]


title: Old_1, Cell 165 of 192 [AS-441542-LR-48372]


title: Old_1, Cell 164 of 192 [AS-441540-LR-48372]


title: Old_1, Cell 167 of 192 [AS-441546-LR-48372]


title: Old_1, Cell 183 of 192 [AS-441578-LR-48372]


title: Old_1, Cell 163 of 192 [AS-441538-LR-48372]


title: Old_1, Cell 162 of 192 [AS-441536-LR-48372]


title: Old_1, Cell 160 of 192 [AS-441532-LR-48372]


title: Plasma cfDNA HU005.12

city: Ein Kerem

first: Benjamin

middle: P

last: Berman

address: Hadassah University Medical Center, Bldg 3 rm 30

country: Israel

institute: Hebrew University of Jerusalem

description: circulating DNA

library_source: genomic

data_processing:
Base calling and alignment: Fast5 files were taken from previous publication (EGAD00001006888), which were generated using real-time high-accuracy basecalling during GridION run. Fast5 files were demultiplexed with guppy_barcoder (Version 5.0.16+b9fcd7b5b) using “--trim_barcodes --barcode_kits EXP-NBD104”. Individual barcode Fast5 files were processed into fastq and bam files using Megalodon v. 2.4.2 with the following command-line parameters “--edge-buffer 0 --mod-min-prob 0 --guppy-params ‘-d /usr/local/hurcs/guppy/6.0.1/data --barcode_kits EXP-NBD104 --trim_barcodes’ --remora-modified-bases dna_r9.4.1_e8 hac 0.0.0 5mc CG 0 --guppy-config dna_r9.4.1_450bps_hac.cfg”. Internally, Megalodon used Guppy server version 6.0.1+652ffd1, and basecalling model r9.4.1_450bps_hac. By default, Megalodon filters out multi-mapping (supplementary) reads and uses the minimap2 “map-ont” mode to filter low quality mappings.
Anonymized BAM files: Individual tile Fast5 files were run individually, and mod_mapping.bam files were merged using samtools merge (v1.14). Samtools/HTSlib versions before v.1.14 do not handle the Mm/Ml modification tages. Because Megalodon reports only the reference sequence in the BAM records, and does not report any base substitutions, these are anonymous BAM files which do not contain any SNP information, and thus contain no personally identifiable information. These are the primary files used for both fragmentomic and methylation analysis. These are the "meg242.remora1.edgefix.mod_mappings.sorted.hg38.bam" files deposited here.
Megalodon methyl bed file extraction: To extract (stranded) methylation information from the mod_mapping.bam files, we used modbam2bed (https://github.com/epi2me-labs/modbam2bed) v.0.4.5, specifying a minimum probability threshold of 0.667, and filtering out positions with 0 confident reads using awk. The full command line was “modbam2bed --cpg -t 4 -a 0.333 -b 0.667 | awk ‘($5>0){print} > out.bed”. All coordinates are in GRCh38 and are 0-based. These files are named “*.meg242.remora1.edgefix.modbam2bed.5mC.cut0.667.hg38.bed”. Column 11 corresponds to the percent of reads methylated. Modbam2bed does not provide a column for the actual number of reads that this percentage is based on, but it can be calculated from the other columns. readCount=(col5*col10)/1000. We also processed this into a simple bedgrpah with just the methylation fraction (beta) values in files named "meg242.remora1.edgefix.modbam2bed.5mC.cut0.667.hg38.sorted.bedgraph". These can be loaded into any genome browser.
DeepSignal methylation calling and processing.  Raw Fast5 files were processed with DeepSignal Version 0.1.8 (4), with model “model.CpG.R9.4_1D.human_hx1.bn17.sn360.v0.1.7+/bn_17.sn_360.epoch_9.ckpt”, which was downloaded from the DeepSignal Google Drive (https://drive.google.com/open?id=1zkK8Q1gyfviWWnXUBMcIwEDw3SocJg7P). We used the DeepSignal call_mods (modification_call) output tsv file, extracting the (strand-specific) methylation calls for each CpG from column 9 (called_label field), and calculated a methylation beta value by taking the number of methylated reads (value 1) divided by the total number of reads (value 0 or value 1). These were collapsed into a bedgraph file with a value between 0-1 for every CpG covered. These are the "sorted.grouped.0based.bedgraph.gz" deposited here.
Assembly: GRCh38
Library strategy: WGS

molecule: genomic DNA

organism: Homo sapiens

source_name: Plasma

extract_protocol:
Blood collected in EDTA tubes, centrifuged 1500g for 10 minutes. cfDNA extracted from 4mL of plasma using QIAamp Circulating Nucleic Acid Kit (QIAGEN, 55114), and stored at -80C before library construction.
Oxford Nanopore SQK-LSK109 (all samples except for 19_326 also used the multiplex protocol NBD-EXP104)

tag: disease state

value: Healthy control


title: Plasma cfDNA HU005.11

description: circulating DNA


title: Old_1, Cell 159 of 192 [AS-441530-LR-48372]


title: Old_1, Cell 155 of 192 [AS-441522-LR-48372]


title: Old_1, Cell 156 of 192 [AS-441524-LR-48372]


title: Old_1, Cell 173 of 192 [AS-441558-LR-48372]


title: Old_1, Cell 157 of 192 [AS-441526-LR-48372]


title: Old_1, Cell 161 of 192 [AS-441534-LR-48372]


title: Old_1, Cell 166 of 192 [AS-441544-LR-48372]


title: Old_1, Cell 158 of 192 [AS-441528-LR-48372]


title: Old_1, Cell 154 of 192 [AS-441520-LR-48372]


title: Old_1, Cell 152 of 192 [AS-441516-LR-48372]


title: Old_1, Cell 153 of 192 [AS-441518-LR-48372]


title: Plasma cfDNA HU005.10

description: circulating DNA


title: Old_1, Cell 147 of 192 [AS-441506-LR-48372]


title: Old_1, Cell 149 of 192 [AS-441510-LR-48372]


title: Old_1, Cell 151 of 192 [AS-441514-LR-48372]


title: Old_1, Cell 148 of 192 [AS-441508-LR-48372]


title: Old_1, Cell 143 of 192 [AS-441498-LR-48372]


title: Old_1, Cell 145 of 192 [AS-441502-LR-48372]


title: Old_1, Cell 146 of 192 [AS-441504-LR-48372]


title: Old_1, Cell 141 of 192 [AS-441494-LR-48372]


title: Old_1, Cell 150 of 192 [AS-441512-LR-48372]


title: Old_1, Cell 142 of 192 [AS-441496-LR-48372]


title: Old_1, Cell 139 of 192 [AS-441490-LR-48372]


title: Old_1, Cell 144 of 192 [AS-441500-LR-48372]


title: UAT-2020-F-015_G05 [CIN14]

type: RNA

city: Esplugues de Llobregat

first: Soledad

last: Gómez-González

state: Barcelona

address: Santa Rosa, 39-57, 4a planta

department: Oncology

country: Spain

institute: Hospital Sant Joan de Déu

hyb_protocol: Standard Affymetrix Protocol.  Standard Affyemtrix protocol.

scan_protocol: Arrays were imaged using standard recommended Affyemtrix scanner setting.

data_processing: Affymetrix CEL files were normalized using Robust Multi-array Average (RMA) method with a log base 2 (log2) from oligo R Package (Carvalho BS, Irizarry RA (2010). “A Framework for Oligonucleotide Microarray Preprocessing.” Bioinformatics, 26(19), 2363-7. ISSN 1367-4803, doi: 10.1093/bioinformatics/btq431.); available through Bioconductor (www.bioconductor.org).

label: biotin

source_name: Mouse neuroblastoma tumor xenograft

label_protocol: Standard Affymetrix Protocol.

growth_protocol: LA-N-1 cells (7.5x106) were resuspended in PBS:Matrigel and inoculated subcutaneously in four to six-week female athymic nude-Fox1 nu/nu mice. Palpable tumors were measured with a digital caliper three times per week. Tumor volume was calculated with the formula LxW2 (L=length, W=width).

extract_protocol: Total RNA was extracted from 8 tumor xenografts exposed to CIN, AC-265347 or their vehicle.

treatment_protocol: Treatment was initiated when tumors reached a volume of 175 mm3. Animals were randomized in three groups to receive CIN (10mg/kg), AC-265347 (10mg/kg) or its vehicle by oral gavage, 5 days per week. Tumors were excised when volume reached 1500 mm3.

tag: tissue

value: Mouse neuroblastoma tumor xenograft

tag: treatment

value: CIN


title: UAT-2020-F-013_F07 [CIN12]


title: Old_1, Cell 138 of 192 [AS-441488-LR-48372]


title: UAT-2020-F-012_C07 [CIN11]


title: UAT-2020-F-011_C05 [CIN10]


title: UAT-2020-F-008_F05 [AC17]

value: AC


title: UAT-2020-F-014_F09 [CIN13]


title: Old_1, Cell 140 of 192 [AS-441492-LR-48372]


title: UAT-2020-F-006_E07 [AC15]


title: UAT-2020-F-007_E09 [AC16]


title: Old_1, Cell 134 of 192 [AS-441480-LR-48372]


title: UAT-2020-F-016_G07 [CIN17]


title: UAT-2020-F-024_H09 [VEH18]

value: VEH


title: UAT-2020-F-005_E05 [AC14]


title: UAT-2020-F-010_B09 [CIN9]


title: UAT-2020-F-021_G09 [VEH14]


title: UAT-2020-F-009_B07 [CIN8]


title: Old_1, Cell 136 of 192 [AS-441484-LR-48372]


title: UAT-2020-F-019_D07 [VEH11]


title: UAT-2020-F-022_H05 [VEH16]


title: UAT-2020-F-018_D05 [VEH10]


title: C. crescentus rtrC overexpression - replicate 3

city: East Lansing

first: Sean

last: Crosson

state: Michigan

address: 567 Wilson Rd

department: Dept. Microbiology and Molecular Genetics

country: USA

institute: Michigan State University

data_processing:
CLC Genomics Workbench v21.0.05
Reads were mapped to extracted genes from CP001340.1 (parameters- mismatch cost: 2, insertion cost: 3, deletion cost: 3, length fraction: 0.8, similarity fraction: 0.8)
Read count extraction and normalization were performed using CLC Genomic Workbench
Assembly: CP001340.1
Supplementary files format and content: RtrC_ExpressionBrowser.csv

organism: Caulobacter vibrioides CB15

source_name: Caulobacter vibrioides CB15

growth_protocol: Strains grown in M2X supplemented with 50 µM cumate at 30°C

extract_protocol:
Cell pellets were resuspended in 1ml TRIzol, then stored at -80°C. Samples were heated at 65°C for 10 min. 0.2 ml chloroform was mixed into samples and incubated for 5 min. Samples pelleted at 17,000xg for 15 min at 4°C. Aqueous layer transferred to fresh tube. 450 µl isopropanol was added and stored at -80°C overnight. Thawed samples were pelleted at 4°C for 30 min, then supernatant was removed. Pellets were washed in 1 ml 70% ethanol, dried, and resuspended in 100 µl RNase-free H2O. Samples were cleaned up with RNeasy Mini Kit (QIAGEN).
RNA was sent to Microbial Genome Sequencing Center for library preparation and sequencing. Libraries were prepared with Illumina Stranded mRNA Prep and RiboZero Plus rRNA depletion.

treatment_protocol: Expressed induced with 50 µM cumate. Cultures were harvested between 0.3-0.4 OD660

value: Caulobacter vibrioides CB15

value: CB15 xylX::pPTM059 (PQ5-rtrC)

value: Expressed induced with 50 microM cumate


title: UAT-2020-F-001_A05 [AC8]


title: UAT-2020-F-023_H07 [VEH17]


title: UAT-2020-F-003_A09 [AC10]


title: UAT-2020-F-002_A07 [AC9]


title: Old_1, Cell 131 of 192 [AS-441474-LR-48372]


title: Old_1, Cell 130 of 192 [AS-441472-LR-48372]


title: Old_1, Cell 135 of 192 [AS-441482-LR-48372]


title: Old_1, Cell 133 of 192 [AS-441478-LR-48372]


title: C. crescentus rtrC overexpression - replicate 2


title: Old_1, Cell 132 of 192 [AS-441476-LR-48372]


title: C. crescentus rtrC overexpression - replicate 1


title: C. crescentus empty vector - replicate 4

value: CB15 xylX::pPTM057 (PQ5-EV)


title: C. crescentus empty vector - replicate 3


title: Old_1, Cell 125 of 192 [AS-441462-LR-48372]


title: 3x-FLAG-rtrC ChIP input replicate 2

data_processing:
Paired reads were mapped to CP001340.1 genome with Bowtie2 v2.3.4.1
PCR duplicates were removed, unpaired reads were removed, and peaks were called with Genrich with significance q<0.05
Assembly: CP001340.1
Supplementary files format and content: encodepeak

growth_protocol: Strains grown in PYE supplemented with 2 µg/ml oxytetracycline at 30°C

extract_protocol:
Crosslinking was induced with 1% formaldehyde and quenched with 0.125 mM glycine. Cells were lysed and DNA was sheared by sonication. Samples were pre-cleared on SureBeads Protein A magnetic beads. 5% of total volume sample was removed as input. Samples were incubated on α-FLAG magnetic beads, then eluted with 100 ng/µl 3xFLAG peptide. Samples were subject to RNase & proteinase K treatment, then purified with the Zymo ChIP DNA clean & concentrator kit.
Sample libraries were prepared using the Illumina DNA Prep kit and IDT 10bp UDI indices, and sequenced on an Illumina NextSeq 2000, producing 2x151bp reads. Demultiplexing, quality control and adapter trimming was performed with bcl-convert

treatment_protocol: Induce 3xFLAG-rtrC expression with addition of 50 µM cumate for 6h at 30°C

value: CB15 pQF-3xFLAG-rtrC

value: Induce 3xFLAG-rtrC expression with 50 µM cumate for 6h


title: C. crescentus empty vector - replicate 2


title: 3x-FLAG-rtrC ChIP input replicate 3


title: UAT-2020-F-017_C09 [VEH9]


title: Old_1, Cell 122 of 192 [AS-441456-LR-48372]


title: UAT-2020-F-004_B05 [AC13]


title: 3x-FLAG-rtrC ChIP output replicate 2


title: Old_1, Cell 128 of 192 [AS-441468-LR-48372]


title: Old_1, Cell 127 of 192 [AS-441466-LR-48372]


title: Old_1, Cell 123 of 192 [AS-441458-LR-48372]


title: Old_1, Cell 124 of 192 [AS-441460-LR-48372]


title: 3x-FLAG-rtrC ChIP output replicate 3


title: C. crescentus empty vector - replicate 1


title: Old_1, Cell 129 of 192 [AS-441470-LR-48372]


title: Old_1, Cell 119 of 192 [AS-441450-LR-48372]


title: Old_1, Cell 118 of 192 [AS-441448-LR-48372]


title: Old_1, Cell 121 of 192 [AS-441454-LR-48372]


title: Old_1, Cell 115 of 192 [AS-441442-LR-48372]


title: JQ1_B4N797_input_Rep1

city: Boston

first: Mitzi

middle: I

last: Kuroda

state: MA

address: 77 Avenue Louis Pasteur

department: Genetics

institute: Brigham and Women's Hospital/Harvard Medical School

description: JQ1 B4N-797 input 1

data_processing:
ChIPseq fastq reads were aligned to the human genome (hg38) with bowtie2.3.4.3
Duplicate reads were removed with samtools1.14
bigwig files were generated with Deeptools3.0.2 using the bamCoverage command and normalized to 1x genome coverage
Assembly: hg38
Supplementary files format and content: bigwig

source_name: 797 BRD4-NUT

extract_protocol:
Cells were formaldehyde fixed in 3.7% formaldehyde. DNA-protein complexes were affinity purified and DNA was extracted as described for the BioTAP-XL procedure in Alekseyenko et al 2015.
NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina (New England Biolabs cat#E6240S)

treatment_protocol: NC797 cells were treated with 500uM JQ1 or DMSO for 12 hours before harvesting

tag: cell line

value: 797 BRD4-NUT

value: N-BioTAP-cMYC


title: JQ1_B4N797_input_Rep2

description: JQ2 B4N-797 input 2


title: DMSO_B4N797_input_Rep2

description: DMSO B4N-797 input 2

value: DMSO


title: UAT-2020-F-020_D09 [VEH12]


title: DMSO_B4N797_MYC-BioTAP_ChIP_Rep2

description: DMSO B4N-797 Myc 12


title: JQ1_B4N797_MYC-BioTAP_ChIP_Rep1

description: JQ1 B4N-797 Myc 1


title: DMSO_B4N797_MYC-BioTAP_ChIP_Rep1

description: DMSO B4N-797 Myc 1


title: Old_1, Cell 113 of 192 [AS-441438-LR-48372]


title: JQ1_B4N797_MYC-BioTAP_ChIP_Rep2

description: JQ1 B4N-797 Myc 12


title: Old_1, Cell 120 of 192 [AS-441452-LR-48372]


title: Old_1, Cell 112 of 192 [AS-441436-LR-48372]


title: Old_1, Cell 126 of 192 [AS-441464-LR-48372]


title: Old_1, Cell 111 of 192 [AS-441434-LR-48372]


title: N4(paracancer)

city: Beijing

first: Chuan

last: Qin

address: No.95,yong an road.

department: Department of Urology

country: China

institute: Beijing Friendship Hospital, Capital Medical University

description: tRNA-Derived Small RNAs

data_processing:
Image analysis and base calling are performed using Solexa pipeline v1.8 (Off-Line Base Caller software, v1.8)
Raw data files in FASTQ format were generated from the Illumina sequencer. To examine the sequencing quality, the quality score plot of each sample was plotted.
Trimmed reads in FASTA format are aligned allowing for 1 mismatch only to the mature tRNA sequences, then reads that do not map are aligned allowing for 1 mismatch only to precursor tRNA sequences with bowtie software
The abundance of tRF & tiRNA are evaluated using their sequencing counts and is normalized as counts per million of total aligned reads (CPM).
The tRFs & tiRNAs  differentially expressed are screened based on the count value with R package edgeR
Genome_build: hg18
Supplementary_files_format_and_content: Tab-delimited text files include counts per million of total aligned reads (CPM), for each Sample

source_name: adjacent control mucosal tissue

extract_protocol:
In total, 4 pairs of MIBC specimens and adjacent control mucosal tissues (with a distance of 3 cm from the tumor) were collected during the surgery and immediately stored in liquid nitrogen to prevent RNA degradation.In brief, the total RNA was isolated using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) based on the manufacturer’s instructions. Before the sequencing experiment, the integrity and quantity of each RNA sample were checked using agarose gel electrophoresis and the NanoDrop ND-1000 (NanoDrop, Wilmington, DE, USA) instrument in terms of quality control.
Sequencing libraries were size-selected for the RNA biotypes to be sequenced using an automated gel cutter. The sequencing library was determined by the Agilent 2100 Bioanalyzer using the Agilent DNA 1000 chip kit (Agilent, part # 5067-1504). tRNA sequences of cytoplasmic were downloaded from GtRNAdb. Only fragments that can be compared with tRNA and pre-tRNA are defined as tsRNAs so that to exclude the influence of other small RNA fragments.

value: adjacent control mucosal tissue

tag: tumor stage

tag: age


title: DMSO_B4N797_input_Rep1

description: DMSO B4N-797 input 1


title: Old_1, Cell 110 of 192 [AS-441432-LR-48372]


title: Old_1, Cell 109 of 192 [AS-441430-LR-48372]


title: Old_1, Cell 117 of 192 [AS-441446-LR-48372]


title: N1(paracancer)

description: tRNA-Derived Small RNAs


title: Old_1, Cell 116 of 192 [AS-441444-LR-48372]


title: 3x-FLAG-rtrC ChIP output replicate 1


title: Old_1, Cell 108 of 192 [AS-441428-LR-48372]


title: N2(paracancer)

description: tRNA-Derived Small RNAs


title: T4(tumor)

description: tRNA-Derived Small RNAs

source_name: bladder cancer tissue

value: bladder cancer tissue


title: N3(paracancer)

description: tRNA-Derived Small RNAs


title: T3(tumor)

description: tRNA-Derived Small RNAs


title: T2(tumor)

description: tRNA-Derived Small RNAs


title: Old_1, Cell 114 of 192 [AS-441440-LR-48372]

value: Lgr5 high-ISCs


title: T1(tumor)

description: tRNA-Derived Small RNAs


title: Old_1, Cell 107 of 192 [AS-441426-LR-48372]


title: Old_1, Cell 106 of 192 [AS-441424-LR-48372]


title: Old_1, Cell 104 of 192 [AS-441420-LR-48372]


title: Old_1, Cell 105 of 192 [AS-441422-LR-48372]


title: Old_1, Cell 101 of 192 [AS-441414-LR-48372]


title: Old_1, Cell 102 of 192 [AS-441416-LR-48372]


title: Old_1, Cell 103 of 192 [AS-441418-LR-48372]


title: Old_1, Cell 100 of 192 [AS-441412-LR-48372]


title: THP-1 CTCF HiChIP 1,25D N2

city: Frankfurt am Main

first: Timothy

last: Warwick

state: Hesse

address: Theodor-Stern-Kai 7

department: Institute for Cardiovascular Physiology

institute: Goethe Universität Frankfurt am Main

description: CTCF HiChIP after 24 h stimulation with 100 nM 1,25(OH)2D3 - biological replicate 2

data_processing:
Library strategy: HiChIP-Seq
HiC-Pro (v2.11.1), default parameters with MboI-digested genome
hichipper with EACH,ALL specified
diffLoop (mango Padj < 0.01, 20 kb minimum loop span, limma/Voom)
Genome_build: hg19
Supplementary_files_format_and_content: Text file of diffLoop output, differential loops vitamin D vs. CTL

source_name: Cell line

extract_protocol:
After treatment of 30 x 106 THP-1 cells, nuclear proteins were cross-linked to genomic DNA by adding formaldehyde directly to the medium to a final concentration of 1% and incubating at room temperature for 10 min on a rocking platform. Cross-linking was stopped by adding glycine to a final concentration of 0.125 M and incubating at room temperature for 10 min on a rocking platform. The cells were collected by centrifugation and washed twice with ice cold phosphate-buffered saline (PBS). Cells were lysed in 1 ml Hi-C Lysis Buffer (10 mM Tris-HCl pH 8.0, 10 mM NaCl, 0.2% NP-40, protease inhibitors) with rotation at 4°C for 30 min and split into two aliquots. Nuclei were washed with Hi-C Lysis Buffer and incubated with 100 µl 0.5% SDS at 62°C for 10 min. SDS was quenched with 285 µl H2O and 50 µl 10% Triton X-100 rotating at 37°C for 15 min. Restriction digestion with 15 µl MboI (New England Biolabs) in NEB Buffer 2.1 was performed rotating at 37°C for 2 h.  The pellet was washed twice with and resuspended in NEB Buffer 2.1. Overhangs were filled in and marked with biotin by adding 37.5 µl 0.4 mM Biotin-14-dATP (Thermo Fisher Scientific), 1.5 µl 10 mM dCTP, 1.5 µl 10 mM dGTP, 1.5 µl 10 mM dTTP and 5 µl 10 U/µl DNA Polymerase I, Large (Klenow) Fragment (Thermo Fisher Scientific) and rotating at 37°C for 1 h. Then, proximity ligation was performed by 4 h rotation after addition of 150 µl 10x NEB T4 DNA ligase buffer with 10 mM ATP (Thermo Fisher Scientific), 125 µl 10% Triton X-100, 3 µl 50 mg/ml BSA, 4 µl 1000 CEU/µl T4 DNA Ligase (Thermo Fisher Scientific), and 666 µl H2O. The supernatant was removed by centrifugation and nuclei were resuspended in 500 µl SDS lysis buffer (50 mM Tris-HCl pH 8.1, 10 mM EDTA, 1% SDS, protease inhibitors). The two aliquots were re-combined and sonicated in a Bioruptor Plus (Diagenode) for 5 min. Then, debris was removed. Chromatin Immunoprecipitation was performed by adding 400 µl chromatin aliquots and 1600 µl IP Dilution Buffer (20 mM Tris-HCl pH 7.5, 2 mM EDTA, 1% Triton X-100, 150 mM NaCl, protease inhibitors) to each 90 µl Dynabeads Protein G (Invitrogen), pre-coupled with 4 µl CTCF antibody (Millipore 07-729), and rotating at 4°C for over night. The next day, the beads were washed 3x with Cell Lysis Buffer (50 mM HEPES-KOH pH 7.5, 1 mM EDTA, 1% Triton X-100, 150 mM NaCl, 0.1% SDS, 0.1% sodium deoxycholate, protease inhibitors), 1x with High Salt Buffer (50 mM HEPES-KOH pH 7.5, 1 mM EDTA, 1% Triton X-100, 350 mM NaCl, 0.1% SDS, 0.1% sodium deoxycholate, protease inhibitors), 1x with ChIP Wash Buffer (10 mM Tris-HCl pH 8.0, 1 mM EDTA, 0.5% NP-40, 250 mM LiCl, 0.5% sodium deoxycholate, protease inhibitors) and 2x with TE Buffer. Two sequential elutions were carried out with 150 µl DNA Elution Buffer (50 mM NaHCO3, 1% SDS) (10 min room temperature, 3 min 37°C) and both eluates from all replicate IPs were pooled. Proteins were digested with Proteinase K and crosslinks were reversed for 2 h at 65°C, followed by DNA purification with the ChIP DNA Clean & Concentrator kit (Zymo Research). Biotin capture was performed with 5 µl washed Dynabeads MyOne Streptavidin C1 (Invitrogen) for 15 min at room temperature. Beads were washed with Tween Wash Buffer (5 mM Tris-HCl pH 7.5, 0.5 mM EDTA, 0.05% Tween-20, 1 M NaCl) and 1x Tagmentation Buffer. Tagmentation with Tn5 transposase (Illumina) in a 50  µl reaction using the appropriate amount of Tn5 transposase for every sample as detailed by Mumbach et al (81) and incubating at 55°C for 10 min. The reaction was stopped by addition of EDTA and beads were washed with Tween Wash Buffer and 10 mM Tris pH 7.5.
Library preparation and size selection were performed as described in the Mumbach protocol

value: Monocyte

tag: agent


title: THP-1 CTCF HiChIP 1,25D N1

description: CTCF HiChIP after 24 h stimulation with 100 nM 1,25(OH)2D3 - biological replicate 1


title: THP-1 CTCF HiChIP CTL N3

description: CTCF HiChIP after 24 h stimulation with vehicle (ethanol) - biological replicate 3

value: vehicle (ethanol)


title: Old_1, Cell 99 of 192 [AS-441410-LR-48372]


title: THP-1 CTCF HiChIP 1,25D N3

description: CTCF HiChIP after 24 h stimulation with 100 nM 1,25(OH)2D3 - biological replicate 3


title: Old_1, Cell 98 of 192 [AS-441408-LR-48372]


title: Old_1, Cell 97 of 192 [AS-441406-LR-48372]


title: Old_1, Cell 96 of 192 [AS-441404-LR-48372]


title: ST3-3

city: Madrid

first: Pilar

last: López Nieva

address: Nicolas Cabrera 1

institute: Centro de Biología Molecular Severo Ochoa

description:
SCP21ID00105
Strand specific total RNA-seq (RF)

data_processing:
Aligment using STAR 2.7.1a with separate files for non-chimeric aligment bam and chimeric bam. It is done to both the R1 and R2 file and for each one of them separately (mate1 R1, mate2 R2).
Detection and quantification of the circulars RNA using Detect Module from Circtools, standard parameters
Genome_build: GRCh37/hg19
Supplementary_files_format_and_content: Excel file with the information about the raw data of the samples
Supplementary_files_format_and_content: a table containing read counts for circRNAs detected. First three columns are chr, circRNA start, circRNA end. From fourth column on are the circRNA read counts, one sample per column, shown in the order given in your samplesheet.
Supplementary_files_format_and_content: circular RNA annotations in BED format
Supplementary_files_format_and_content: host gene expression count table, same setup with CircRNACount file
Supplementary_files_format_and_content: circSkip junctions. The first three columns are the same as in LinearCount/CircRNACount, the following columns represent the circSkip junctions found for each sample

source_name: Thymocytes

growth_protocol: Human postnatal thymocytes were isolated from thymuses removed during cardiac surgery of 7 pediatric patients aged between 1 month and 4 years old

extract_protocol:
Total RNA was obtained using TriPure Reagent (Roche Applied Science, Indianapolis, IN, USA), following manufacturer’s instructions, and treated to removal Ribosomal RNA
High-quality RNA samples (RNA integrity Numbers (RIN) in the range of 7.2–9.8) were used for high-depth Illumina total RNA sequencing (RNA-seq) after ribosomal depletion, with 3 replicates for each thymocyte population (NIMGenetics and Helix BioS, Scientific Park of Madrid, Spain). Image analysis, per-cycle basecalling and quality score assignment were made using Illumina Real Time Analysis software (Illumina, San Diego, CA). BCL files were converted to FASTQ format using Illumina’s Off-Line Basecaller package. RNA-seq libraries resulting from these analyses were sequenced in paired-end format in two different rounds (Illumina HiSeq2000, Illumina Inc., San Diego, CA, USA), leading to 50 bp and 76 bp reads (the latter were trimmed to 50 bp). RNA-seq reads were analysed with the nextpresso pipeline. The quality of sequenced reads was checked with FastQC. Quantification and annotation of circular RNAs and their linear counterparts, was performed using circTools, since it is one of the few bioinformatics tools that allows the alignment of the sequences mates separately, allowing the detection of exonic, exonic-intronic, and intergenic circRNAs and helping to perform internal controls on the sample itself for better identification of chimeric junctions.

treatment_protocol: Human thymic populations were sorted and three differential thymocyte populations were included in the study, as follows: double negative early immature CD34+ (ST1, Stage 1); double positive (CD1A+CD4+CD8+) (ST2, Stage 2); and single positive thymocytes (CD1A-CD4+ and CD1A-CD8+) (ST3, Stage 3). Thymocytes were sorted using magnetic activated cell sorting with autoMACS Pro (Miltenyi Biotec). To isolate early immature thymocytes (ST1), thymocyte suspensions were firstly enriched in CD34+ thymocytes by using the sheep red blood cell technique5. Then, early immature thymocytes were isolated as CD34+ cells with appropriate Ig-coated magnetic MicroBeads. Double Positive thymocytes were directly isolated as CD1A+ cells with CD1A+ MicroBeads. Single positive thymocytes were isolated by removing CD1A- cells then selected with either CD4+ MicroBeads or CD8+ MicroBeads. The purity of the three isolated thymocyte populations was determined by flow cytometry using a FACSCalibur cytometer (Becton Dickinson, San Diego, CA) and the following antibodies: CD34-PE, CD8-APC and CD4-PE (all from MACS Miltenyi Biotec), and CD3-FITC (Beckman Coulter). Purity varied from 95 to 80 per cent. Institutional review board approval was obtained for these studies (CEI 98-1825). The participants provided written informed consent in accordance with the Declaration of Helsinki.

value: Thymocytes

tag: differentiation stage

value: Late Cortical (SP-mix)

tag: immmunophenotype

value: CD4+ or CD8+


title: ST3-2

description:
SCP21ID00104
Strand specific total RNA-seq (RF)


title: ST2-2

description:
SCP21ID00101
Strand specific total RNA-seq (RF)

value: Early Cortical (DP-mix)


title: ST2-3

description:
SCP21ID00102
Strand specific total RNA-seq (RF)


title: Old_1, Cell 95 of 192 [AS-441402-LR-48372]


title: THP-1 CTCF HiChIP CTL N1

description: CTCF HiChIP after 24 h stimulation with vehicle (ethanol) - biological replicate 1


title: THP-1 CTCF HiChIP CTL N2

description: CTCF HiChIP after 24 h stimulation with vehicle (ethanol) - biological replicate 2


title: ST1-2

description:
SCP21ID00098
Strand specific total RNA-seq (RF)

value: Immatures (CD34-mix)


title: ST2-1

description:
SCP21ID00100
Strand specific total RNA-seq (RF)


title: REM15, dilution 1:16, set 4, replicate 3

city: Kuopio

first: Sami

last: Heikkinen

address: Yliopistonranta 1E

department: Institute of Biomedicine

country: Finland

institute: University of Eastern Finland

data_processing:
Using Qiagen's GeneGlobe Data Analysis Center according to manufacturer´s instructions: trimming.
Using Qiagen's GeneGlobe Data Analysis Center according to manufacturer´s instructions: alignment to GRCh38 reference genome using STAR.
Using Qiagen's GeneGlobe Data Analysis Center according to manufacturer´s instructions: counting of unique molecular identifiers (UMIs) for 386 genes including 6 genomic DNA controls and 10 reference genes.
Raw UMI counts were combined with gene and sample annotations in R (version 3.6.1) (R Core Team, 2017) using SummarizedExperiment (version 1.16.1).
For uses other than differential gene expression (DEG) analysis, read counts were normalized using R function DESeq2::varianceStabilizingTransformation (vst) in “blind” mode (version 1.26.1).
To identify technical bias, quality control and exploration were performed (multidimensional scaling, principal component analysis, and unsupervised hierarchical clustering) in R/Bioconductor.
Statistically differentially expressed genes (DEGs) were identified using R package DESeq2 (version 1.26.1), using Wald as test type, FDR for p-value adjustment and the R function DESeq2::lfcShrink (type=”normal”) for shrinking fold changes of low expressed RNAs.
Genome_build: GRCh38
Supplementary_files_format_and_content: Tab-delimited text files include raw read counts for each gene for a given Sample
Supplementary_files_format_and_content: Matrix of vst-normalized counts for every gene (gene_id, gene_name and control_type given) and every sample

source_name: normal human bronchial epithelial cells

growth_protocol: Human airway constructs were differentiated from normal human bronchial epithelial (NHBE) cells (cat. CC-2540, Lonza) in four different sets. The NHBE cells were seeded > 3500 cells/cm2 into cell culture flasks containing LifeFactors® BronchiaLife™ tracheal epithelial cell culture medium (cat. LS-1047) and necessary supplements, and incubated +37 ºC, 5% CO2 for four days. Cells were detached with 0.05% trypsin/EDTA solution and neutralized using trypsin neutralizing solution. Cells were seeded ~140 000 cells/cm2 to Transparent ThinCert™ cell culture inserts pre-treated with 30 µg/mL rat tail collagen type I, and cultured for three days. Culture medium was changed daily. Cell cultures were transferred to air-liquid interface (ALI) in HBTEC Air-Liquid Interface Differentiation Medium including antimicrobial supplement Gentamicin 30 mg/mL and Amphotericin B 15 µg/mL, and cultured for 22 days before the exposure. Culture medium was changed every other day.

extract_protocol:
RNA was extracted using Qiagen RNeasy Plus Mini Kit (Qiagen) including DNase treatment. RNA samples were sent to Sequencing Unit of Finnish Institute for Molecular Medicine (FIMM, Helsinki, Finland) for further RNA quality control and sequencing. RNA quality assessment, library preparation for sequencing, sequencing of RNA amplicons, and data analysis were done according to manufacturer´s instructions (Nordberg et al. 2020).
QIAseq Targeted RNA panel Human Molecular Toxicology Transcriptome (Qiagen) of 386 genes was used for first strand synthesis, molecular barcoding, gene-specific amplification, sample indexing, and library preparation for targeted RNA sequencing according to manufacturer´s instructions.

treatment_protocol: PM sampled with the NIOSH BC251 bio aerosol cyclone sampler were combined with 2 mL of synthetic lung lining fluid (LLF) (Nordberg et al. 2020), mixed, sonicated, and diluted in LLF to three dose levels: 1:16, 1:8, and 1:4. The airway constructs were exposed to the three doses in air liquid interface (+37 °C, 5% CO2, exposure time 24 h). The exposure experiments were conducted in four sets during four months. Paired index and reference houses were always included in the same exposure set.

value: NHBE

tag: set

tag: subset

tag: replicate

value: FALSE

value: likely

value: likely or highly likely


title: Old_1, Cell 92 of 192 [AS-441396-LR-48372]


title: ST1-3

description:
SCP21ID00099
Strand specific total RNA-seq (RF)


title: Old_1, Cell 91 of 192 [AS-441394-LR-48372]


title: ST1-1

description:
SCP21ID00097
Strand specific total RNA-seq (RF)


title: 3x-FLAG-rtrC ChIP input replicate 1

value: Caulobacter vibrioides CB15

value: CB15 pQF-3xFLAG-rtrC

value: Induce 3xFLAG-rtrC expression with 50 µM cumate for 6h


title: C. crescentus rtrC overexpression - replicate 4

value: CB15 xylX::pPTM059 (PQ5-rtrC)

value: Expressed induced with 50 microM cumate


title: Old_1, Cell 90 of 192 [AS-441392-LR-48372]

value: Lgr5 high-ISCs