title: Human Aortic Endothelial Cell_Normal Glucose (NG)_1 hour_replicate_4


title: ST-00066487

status: Public on May 21 2022

type: RNA

city: Boston

first: Vrushali

middle: Dipak

last: Fangal

state: MA

address: 181 Longwood Avenue

department: Brigham and Women's Hospital

country: USA

institute: Harvard Medical School

hyb_protocol: Standard Illumina hyb protocol

scan_protocol: Standard Illumina scanning protocol

data_processing: Whole-blood gene expression profiles were generated with 47,323 probes from the HumanHT-12 v4 Expression BeadChip (Illumina, Inc.) that passed stringent and commonly used quality control (QC) metrics. Expression data were log2-transformed and quantile-normalized as a single batch using the lumiT() and lumiN() functions, respectively, from the R package lumi (version 2.22; The R Foundation). Principal components of gene expression were generated using the getPCAFunc function from the R package iCheck (version 0.6). Prior to downstream statistical analyses, we applied a standard nonspecific variance filter to the expression data using the nsFilter function from the R package genefilter (version 1.52). Probes not annotated with a valid Entrez gene identifier or Human Genome Organization gene symbol and probes with interquartile ranges of expression variance below the 50th percentile were removed to select only the most informative probes. Data were then collapsed to a single probe per gene based on the largest interquartile range of expression variance.

molecule: total RNA

organism: Homo sapiens

source_name: children between 6 to 14 years of age with moderate persistent asthma

label_protocol: Standard Illumina labeling protocol

extract_protocol: RNA was extracted from subset of 321 children and blood samples were processed within 4 hrs. of extraction. RNA was stored in PAXgene tubes (Qiagen).

tag: condition

value: asthma

tag: tissue

value: Blood

tag: gender

value: M


title: ST-00066501

value: F


title: ST-00066512


title: ST-00066464


title: Donor 5 CD4 Day 0

type: SRA

city: San Francisco

first: Vivasvan

last: Vykunta

state: CA

address: 513 Parnassus Avenue HSW901

institute: Gladstone-UCSF Institute of Genomic Immunology

library_source: transcriptomic

data_processing:
PE reads processed with kallisto using GRCh37 (hg19) cDNA annotation from ensembl
Transcript counts aggregated at the gene level (ensembl transcript IDs mapped to ensembl gene IDs)
Genes of interest were selected from the normalized and aggregated counts table
Genes with multiple ensembl IDs were merged (summed) based on gene symbol; these "isoforms" showed extremely high correlation (see plots)
Output matrix of TPM values of samples vs gene symbols
Assembly: GRCh37 (hg19)
Supplementary files format and content: tab-delimited text file includes RPKM values for each Sample

source_name: CD4 T cell

extract_protocol:
CD4 and CD8 T cells from each donor were sorted by FACS and then frozen in RNAlater (QIAGEN) for bulk RNA sequencing. Total RNA from frozen samples was extracted using Rneasy Mini Kit (Qiagen) based on manufactuter protocols.
RNA libraries were contructed with Illumina TruSeq RNA Sample Prep Kit v2 (cat. no. RS-122-2001) according to the manufacturer’s protocol.
Bulk RNA Sequencing

tag: individual

value: Donor 5

tag: cell type

value: CD4 T cell

tag: time

value: Day 0

tag: treatment

value: day of electroporation


title: Human Aortic Endothelial Cell_Normal Glucose (NG)_1 hour_replicate_2

city: Abu dhabi

first: Youssef

last: Idaghdour

state: Abu dhabi

address: Saadiyat Marina District

department: Biology

country: United Arab Emirates

institute: NYUAD

data_processing:
Raw sequencing reads were quality controlled using the FASTQC tool prior to processing using Trimmomatic v0.36 to remove barcodes, adapter sequences and low-quality bases (Phred quality score <22).
Filtered reads were then mapped to the human reference genome (Ensembl GRCh38 release-84) using STAR v2.5.2a.
Genes not expressed or with very low expression levels (less than five reads in 2 or more samples in any given condition) were removed.
Filtered raw counts were log2 transformed prior to normalization using JMP Genomics 8 (SAS Institute). Data were batch normalized to reduce any potential technical batch effects.

source_name: Human Aortic Endothelial Cell

growth_protocol: Human aortic endothelial cells (HAECs) obtained from Lonza were grown in normal physiological levels of glucose (NG) (5.5 mM D-glucose) EGM™ 2 Endothelial Cell Growth Medium-2 BulletKit™ (Lonza) containing 2 % fetal bovine serum (FBS) as per manufacturer’s instructions, and maintained at 37°C, 5% CO2

extract_protocol:
Total RNA was isolated from HAECs using the RNeasy Mini Kit (Qiagen) according to manufacturer’s instructions.
KAPA Stranded RNA-Seq kit with RiboErase

treatment_protocol: HAECs were then exposed to NG EGM™ 2 Endothelial Cell Growth Medium-2 BulletKit™ (Lonza) containing reduced serum (0.2 % FBS) overnight (growth factors and supplements remained in the media). HAECs were then treated with EGM™ 2 Endothelial Cell Growth Medium-2 BulletKit™ (Lonza) containing 2 % FBS and either 5.5 mM D-glucose or 25 mM D-glucose. HAECs were then harvested at 0, 0.5, 1, 4 and 8 h post treatment.

value: Endothelial cell

value: Normal Glucose (5.5 mM D-glucose)

value: 1 hour


title: ST-00066497


title: Human Aortic Endothelial Cell_Normal Glucose (NG)_1 hour_replicate_1


title: ST-00066554


title: Donor 5 CD8 Day -2

source_name: CD8 T cell

value: CD8 T cell

value: Day -2

value: pre-stimulation


title: ST-00066475


title: ST-00066414


title: Human Aortic Endothelial Cell_Normal Glucose (NG)_05 hour_replicate_4

value: 0.5 hour


title: ST-00066451


title: Donor 4 CD8 Day 2

value: Donor 4

value: Day 2

value: after electroporation


title: ST-00066400

value: asthma

value: Blood


title: Human Aortic Endothelial Cell_Normal Glucose (NG)_05 hour_replicate_2


title: ST-00066385

value: F


title: Human Aortic Endothelial Cell_Normal Glucose (NG)_05 hour_replicate_3


title: ST-00066417


title: Donor 4 CD8 Day 0


title: Human Aortic Endothelial Cell_Normal Glucose (NG)_0 hour_replicate_4

value: 0 hour


title: Donor 4 CD4 Day 2

value: CD4 T cell


title: ST-00066377

value: M


title: Donor 5 CD4 Day -2

value: Donor 5


title: ST-00066374


title: ST-00066368


title: Human Aortic Endothelial Cell_Normal Glucose (NG)_05 hour_replicate_1

value: Endothelial cell


title: Donor 4 CD4 Day 0

value: Day 0

value: day of electroporation


title: Donor 4 CD8 Day -2

value: CD8 T cell


title: ST-00066354


title: Human Aortic Endothelial Cell_Normal Glucose (NG)_0 hour_replicate_3

value: Normal Glucose (5.5 mM D-glucose)


title: ST-00066349


title: ST-00066339


title: ST-00066419


title: Human Aortic Endothelial Cell_Normal Glucose (NG)_0 hour_replicate_2


title: RAW 264.7 macrophages,VLDLsep, replicate 2

city: Wageningen

first: Guido

last: Hooiveld

address: HELIX, Stippeneng 4

department: Div. Human Nutrition & Health

country: Netherlands

institute: Wageningen University

data_processing:
Sequencing reads that contained adapters and/or had low quality, or aligned to rRNA, were filtered off before mapping.
The tool Salmon (version 0.14.1) was used to map the cleaned reads to the GRCm38.p6 mouse genome assembly-based transcriptome sequences as annotated by the GENCODE consortium (release M22). Salmon parameters used: -i ./salmon_index_M22  --libType A --seqBias --gcBias --biasSpeedSamp 5 --validateMappings --numGibbsSamples 20
The Salmon-obtained transcript abundance estimates and lengths were imported in R using the package tximport (version 1.12.3) to estimate gene level counts.
Assembly: GRCm38.p6
Supplementary files format and content: Matrix table with raw gene counts for every gene and every sample (gene level counts were calculated from the Salmon-obtained transcript abundance estimates)
Supplementary files format and content: R-object (txi.counts.annotated.GEO.Rdata; exported tximport object) containing gene level counts estimated from the Salmon-obtained transcript abundance estimates. Note: count data was not transformed nor scaled (i.e. countsFromAbundance = "no"), and can be used for subsequent analysis in edgeR or DESeq2.
Supplementary files format and content: R object (txi.lengthScaledTPM.annotated.GEO.Rdata; exported tximport object) containing scaled gene level counts estimated from the Salmon-obtained transcript abundance estimates. Note: count data was scaled using library size and transcript gene length (i.e. countsFromAbundance = "lengthScaledTPM"), and these scaled counts can be used for subsequent analysis in limma (voom function).

organism: Mus musculus

source_name: RAW 264.7 macrophages, treated with VLDL-sized emulsion particles (VLDLsep) for 6 hrs

growth_protocol: RAW 264.7 macrophages were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Lonza) supplemented with 1% penicillin/streptomycin (PS) and 10% fetal bovine serum  (FBS). RAW 264.7 cells were used between passage numbers 13 and 15.

extract_protocol:
Total RNA from all cell culture samples was extracted using TRIzol reagent (Thermo Fisher Scientific) and purified using the Qiagen RNeasy Mini kit (Qiagen) according to manufacturer’s instructions. RNA concentration was measured with a Nanodrop 1000 spectrometer and RNA integrity was determined using an Agilent 2100 Bioanalyzer with RNA 6000 microchips (Agilent Technologies).
Library construction and RNA sequencing on BGISEQ-500 were conducted at Beijing Genomics Institute (BGI) for pair-end 150bp runs. At BGI, Genomic DNA was removed with two digestions using Amplification grade DNAse I (Invitrogen). The RNA was sheared and reverse transcribed using random primers to obtain cDNA, which was used for library construction. The library quality was determined by using Bioanalyzer 2100 (Agilent Technologies). Then, the library was used for sequencing with the sequencing platform BGISEQ-500 (BGI).

treatment_protocol: RAW macropahges were treated with 1 mM VLDLsep and CHYLsep for 6 hours. Cells were washed twice after the treatments. Untreated cells served a control.

tag: cell line

value: 1 mM VLDLsep

tag: exposure time

value: 6 hrs


title: RAW 264.7 macrophages,VLDLsep, replicate 3


title: RAW 264.7 macrophages,VLDLsep, replicate 1


title: ST-00066329


title: ST-00066189


title: ST-00066223


title: ST-00066174


title: ST-00066335


title: ST-00066304


title: RAW 264.7 macrophages, CHYLsep, replicate 3

source_name: RAW 264.7 macrophages, treated with chylomicron-sized emulsion particles (CHYLsep) for 6 hrs

value: 1 mM CHYLsep


title: Human Aortic Endothelial Cell_Normal Glucose (NG)_0 hour_replicate_1


title: Human Aortic Endothelial Cell_High Glucose (HG)_8 hour_replicate_4

value: High Glucose (25 mM D-glucose)

value: 8 hour


title: Donor 3 CD4 Day 2

value: Donor 3

value: Day 2

value: after electroporation


title: ST-00066168

value: asthma

value: Blood


title: ST-00066176

value: F


title: RAW 264.7 macrophages, CHYLsep, replicate 2


title: Donor 4 CD4 Day -2

value: Donor 4

value: CD4 T cell

value: Day -2

value: pre-stimulation


title: Donor 3 CD8 Day 2

value: CD8 T cell


title: RAW 264.7 macrophages, CHYLsep, replicate 1


title: Human Aortic Endothelial Cell_High Glucose (HG)_8 hour_replicate_3

value: Endothelial cell


title: Human Aortic Endothelial Cell_High Glucose (HG)_8 hour_replicate_2


title: Donor 3 CD8 Day 0

value: Day 0

value: day of electroporation


title: RAW 264.7 macrophages, untreated, replicate 2

source_name: RAW 264.7 macrophages, untreated

value: untreated, control

value: 6 hrs


title: Donor 3 CD4 Day 0


title: RAW 264.7 macrophages, untreated, replicate 3


title: ST-00066130


title: ST-00066155

value: M


title: Donor 3 CD8 Day -2

value: Donor 3


title: Human Aortic Endothelial Cell_High Glucose (HG)_8 hour_replicate_1

value: High Glucose (25 mM D-glucose)

value: 8 hour


title: Human Aortic Endothelial Cell_High Glucose (HG)_4 hour_replicate_4

value: 4 hour


title: ST-00066063

value: asthma

value: Blood


title: RAW 264.7 macrophages, untreated, replicate 1


title: Donor 3 CD4 Day -2


title: PBS_3h_rep3

city: Shanghai

first: chen

middle: xiao

last: qing

state: 上海

address: Life Science Laboratory Building, 320 Yueyang Road, Xuhui District,

department: Institut Pasteur of Shanghai ,CAS

country: China

institute: Pathogenic Fungal Infection and Host Immunity Research Group

data_processing:
Illumina Casava1.7 software used for basecalling.
Quality trimming and adapter trimming were performed by Skewer (V0.2.2). Filtered reads were mapped with STAR version 2.7.4. to the mouse genome
The expression profile of each gene was obtained by StringTie (V2.1.2). Each gene expression was normalized by DESeq2 (V1.20), followed by differential expression analysis.
Assembly: mm10
Supplementary files format and content: Matrix table with raw gene counts for every gene and every sample

source_name: BMDM

extract_protocol:
BMDMs (1 x10^6 in 6-well plates) were infected with C. albicans or C. auris cells (MOI=5) for 1 and 3 h, respectively, and flash-frozen in liquid nitrogen. Total RNA was extracted using TRIZOL (Invitrogen) according to the manufacturer’s directions and stored at -80 ̊C.
RNA libraries were prepared for sequencing using standard Illumina protocols

tag: strain

value: BMDM


title: SC5314_3h_rep3


title: Donor 2 CD8 Day 2

value: Donor 2

value: Day 2

value: after electroporation


title: Human Aortic Endothelial Cell_High Glucose (HG)_4 hour_replicate_3


title: Donor 2 CD4 Day 2

value: CD4 T cell


title: Human Aortic Endothelial Cell_High Glucose (HG)_4 hour_replicate_2

value: Endothelial cell


title: Donor 2 CD8 Day 0

value: CD8 T cell

value: Day 0


title: PBS_3h_rep1


title: ST-00066000

value: F


title: Human Aortic Endothelial Cell_High Glucose (HG)_4 hour_replicate_1


title: Human Aortic Endothelial Cell_High Glucose (HG)_24 hour_replicate_4

value: 24 hour


title: SC5314_6h_rep1


title: Donor 2 CD4 Day 0

value: day of electroporation


title: Human Aortic Endothelial Cell_High Glucose (HG)_24 hour_replicate_3


title: Donor 2 CD8 Day -2

value: Day -2

value: pre-stimulation


title: BJCA001_6h_rep1


title: ST-00065988

value: M


title: Human Aortic Endothelial Cell_High Glucose (HG)_24 hour_replicate_2

value: High Glucose (25 mM D-glucose)


title: ST-00065927

value: asthma


title: ST-00065996

value: Blood


title: ST-00065911


title: ST-00065938


title: Donor 2 CD4 Day -2


title: ST-00065904


title: ST-00065917


title: PBS_3h_rep2

value: BMDM


title: Human Aortic Endothelial Cell_High Glucose (HG)_24 hour_replicate_1


title: Donor 1 CD8 Day 2

value: Donor 1


title: SC5314_6h_rep2


title: ST-00065903


title: ST-00065886


title: BJCA001_6h_rep2


title: Human Aortic Endothelial Cell_High Glucose (HG)_1 hour_replicate_4

value: 1 hour


title: Donor 1 CD4 Day 2

value: Day 2

value: after electroporation


title: BJCA001_3h_rep1


title: Human Aortic Endothelial Cell_High Glucose (HG)_1 hour_replicate_3

value: Endothelial cell


title: Human Aortic Endothelial Cell_High Glucose (HG)_1 hour_replicate_2


title: Donor 1 CD8 Day 0

value: CD8 T cell


title: Donor 1 CD4 Day 0

value: CD4 T cell

value: Day 0


title: BJCA001_6h_rep3


title: Donor 1 CD8 Day -2


title: PBS_6h_rep2


title: PBS_6h_rep3_


title: Human Aortic Endothelial Cell_High Glucose (HG)_05 hour_replicate_4

value: 0.5 hour


title: ST-00065880


title: ST-00065851


title: ST-00065824

value: F


title: ST-00065645


title: ST-00065731


title: Human Aortic Endothelial Cell_High Glucose (HG)_05 hour_replicate_3


title: Human Aortic Endothelial Cell_High Glucose (HG)_1 hour_replicate_1


title: N4

first: Zhou

last: haiwen

address: 500 Qu-xi Road, Shanghai

department: Oral Mucosal Diseases

institute: Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine

description:
processed_data_control_samples.xlsx
GSM3765315

data_processing:
Basecalls performed using CASAVA version 1.8
circRNA-seq reads were mapped to human genome by BWA-MEM software by default parameters except -T = 19
circRNAs were detected and annotated with CIRI software
High throughput sequencing service was provided by CloudSeq Biotech (Shanghai, China). Transcriptome high throughput sequencing and subsequent bioinformatics analysis were all done by Cloud-Seq Biotech (Shanghai, China). Briefly, total RNA was used for removing the rRNAs using Ribo-Zero rRNA Removal Kits (Illumina, USA) following the manufacturer's instructions. RNA libraries were constructed by using rRNA-depleted RNAs with TruSeq Stranded Total RNA Library Prep Kit (Illumina, USA) according to the manufacturer’s instructions. Libraries were controlled for quality and quantified using  the BioAnalyzer 2100 system (Agilent Technologies, USA).Libraries were denatured as single-stranded DNA molecules, captured on Illumina flow cells, amplified in situ as clusters and finally sequenced for 150 cycles on Illumina HiSeq Sequencer according to the manufacturer’s instructions.
Paired-end reads were harvested from Illumina HiSeq 4000 sequencer, and were quality controlled by Q30. After 3’ adaptor-trimming and low quality reads removing by cutadapt software (v1.9.3). The high quality trimmed reads were used to analyse circRNAs. The high quality reads were aligned to the reference genome/transcriptome with STAR software (v2.5.1b) and circRNAs were detected and identified with DCC software (v0.4.4). edgeR software (v3.16.5) was used to normalized the data and perform differentially expressed circRNA analysis.
Genome_build: hg19
Supplementary_files_format_and_content: excel format with mumber of raw junction read

source_name: healthy oral mucosa tissue

growth_protocol: All tissue samples were immediately frozen in liquid nitrogen after resection and stored at -80℃ until RNA retraction

extract_protocol:
RNA extraction protocol
library construction protocol

tag: disease state

value: healthy control

value: fresh

value: oral mucosa


title: SR-3420_2

city: Odense M

first: Lasse Kruse

last: Markussen

address: Campusvej 55

department: BMB

country: Denmark

institute: University of Southern Denmark

data_processing:
Sequencing reads were mapped to the mouse genome mm9 or hg19 using STAR (v2.4.2a), tag counts were summarized at the gene level using HOMER, allowing only one read per position per length. Quantification of the number of mapped reads of each gene was performed using iRNA-seq (v1.1) specifying the -count gene option. Data analysis was performed in R studio (v2022.2.0)
Codes and scripts used to process and analyze data have been deposited to GitHub: https://github.com/LasseKMarkussen/Lipolysis-regulates-major-transcriptional-programs-in-brown-adipocytes
Assembly: hg19
Supplementary files format and content: Human_SR3420.txt contains raw gene counts for the entire dataset.

source_name: Human brown preadipocyte cell line

growth_protocol: Briefly, TERT-hBA cells were cultured in advanced DMEM/F12 basal medium (Gibco, #11320033) supplemented with 10% FBS, 2mM L-glutamine (Gibco, #25030081), 1% penicillin/streptomycin and 2.5ng/ml FGF2 (Gibco, #PHG0367). Two days post confluence (day 0), TERT-hBA cells were induced to differentiate in advanced DMEM/F12 basal medium supplemented with 2% FBS, 2mM L-glutamine, 1% penicillin/streptomycin, 5µg/ml insulin, 1µM dexamethasone, 0,5mM 3-isobutyl-1-methylxanthine, 1nM T3 and 1µM rosiglitazone. On day 3, the medium was refreshed with exactly same medium used on day 0. At day 6 and 9 only T3 was added. At day 12, the adipocytes were considered mature.

extract_protocol:
total RNA was harvested using TRIzol reagent and isolated using the chloroform-isopropanol method. cDNA was transcribed using the QuantiTect Reverse Transcription Kit (Qiagen, #205311).
RNA-seq was performed according to the instructions by the manufacturer (TruSeq2, Illumina) using 500ng of total RNA for preparation of cDNA libraries.

treatment_protocol: Mature D12 TERT-hBA cells were treated with 10uM SR-3420 or Vehicle (DMSO) for 3hrs before harvest.

value: Human brown preadipocyte cell line

value: TERT-hBA

value: Immortalized human brown preadipocyte cell line

tag: genotype

value: WT


title: SR-3420_3


title: SR-3420_1


title: SC5314_3h_rep1

value: BMDM


title: Vehicle_3

value: DMSO


title: Human Aortic Endothelial Cell_High Glucose (HG)_05 hour_replicate_2

value: High Glucose (25 mM D-glucose)


title: BJCA001_3h_rep2


title: N2

description:
processed_data_control_samples.xlsx
GSM3765313


title: N3

description:
processed_data_control_samples.xlsx
GSM3765314


title: Vehicle_2


title: SC5314_6h_rep3


title: Vehicle_1


title: wt1

city: Solna

first: Igor

last: Cervenka

address: Solnavägen 9

department: Physiology and Pharmacology

country: Sweden

institute: Karolinska Institutet

data_processing:
Illumina Casava1.7 software used for basecalling
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to GRCm38 whole genome using STAR 2.5.0a with parameters --outFilterMismatchNmax 33 --alignEndsType Local --alignSJoverhangMin 8 --outFilterScoreMinOverLread 0 --outFilterMatchNminOverLread 0 --outFilterMatchNmin 0 --seedSearchStartLmax 12
Gene counts were obtained from bam files using featureCounts v2.0.0 summarised at gene level
Differential gene expression at FDR level 0.05 was obtained using DESeq2 R package, genes with total raw counts less then 10 across all samples were excluded from analysis
Assembly: GRCm38.p6
Supplementary files format and content: raw gene counts for each sample in tab delimited txt file
Supplementary files format and content: comma-delimited file of differential expression between wild-type and transgenic mice

source_name: Wild-type

extract_protocol:
Gastrocnemius muscle was flash frozen, pulverized and RNA was harvested using Trizol reagent.
RNA libraries were prepared for sequencing using standard Illumina protocols

value: gastrocnemius muscle

tag: Sex

value: Male

value: wild-type (wt)


title: wt2


title: PBS_6h_rep1


title: wt3


title: Donor 1 CD4 Day -2

value: Donor 1

value: CD4 T cell

value: Day -2

value: pre-stimulation


title: N5

description:
processed_data_control_samples.xlsx
GSM3765316

value: healthy control


title: BJCA001_3h_rep3


title: N1

description:
processed_data_control_samples.xlsx
GSM3765312

value: fresh


title: a3.3

source_name: HSA-PGC-1a3 transgenic

value: alpha3_tg (a3)


title: N0

description:
processed_data_control_samples.xlsx
GSM3765311

value: oral mucosa


title: SC5314_3h_rep2


title: a3.2


title: a3.1


title: P4

description: CircRNA Expression Profiling

source_name: oral lichen planus tissue

value: oral lichen planus


title: P6

description: CircRNA Expression Profiling


title: GFP-2

city: Jamaica Plain

first: Xiaoran

last: Zhang

address: 14 Perkins Square, Apt 11

department: Cardiology

institute: Boston Children's Hospital

data_processing:
alignment by STAR to mm10
generate reads count matrix with FeatureCount
Differential expression analysis by DESeq2
Genome_build: mm10
Supplementary_files_format_and_content: ATF6-XBP1-OE-counts: reads count matrix
Supplementary_files_format_and_content: DESeq_XBP and DESeq_ATF6: DESeq2 analysis result

molecule: polyA RNA

source_name: primary cardiomyocyte

extract_protocol:
PureLink RNA Micro Scale Kit
SMART Seq v4 kit, Nextera XT library construction kit

treatment_protocol: AAV treatment of mice, Langendorff isolation of cardiomyocytes, FACS purification of cardiomyocytes

value: cardiomyocyte

value: AAV-GFP

tag: devolopment stage

value: 4 week old


title: ATF6-2


title: ATF6-1


title: P2

description: CircRNA Expression Profiling


title: XBP1-2


title: P3

description: CircRNA Expression Profiling


title: P1

description: CircRNA Expression Profiling


title: XBP1-1


title: P5

description: CircRNA Expression Profiling


title: KO7

city: Beijing

first: Yuxuan

last: Guo

address: 38 Xueyuan Road

institute: Peking University

description:
reads_counts_RYR2
LIB034354_TRA00123610_S14

data_processing:
alignment by STAR to mm10
generate reads count matrix with FeatureCount
Differential expression analysis by DESeq2
Supplementary_files_format_and_content: reads_counts_RYR2: reads count matrix
Supplementary_files_format_and_content: Ryr2c_DESeq: DESeq2 analysis result

source_name: heart

value: heart

tag: developmental stage


title: GFP-1


title: MU_H3K4me3_input2

city: Hangzhou

first: Kun

address: 866 Yuhangtang Rd

institute: Zhejiang University

library_source: genomic

data_processing:
Basecalls performed using CASAVA.
Reads were trimmed by Trimmomatic to remove the bases with low quality and adapter sequences.
The trimmed reads were align to mm10 by Bowtie2.
Reads with low map quality were removed by Samtools, and PCR duplicates were removed by Picard.
The peaks were called by MACS2 with the parameters -p 1e-5 --nomodel --broad.
Genome_build: mm10 (GRCm38)
Supplementary_files_format_and_content: *.bw: Bigwig files are generated using bamCoverage mode of DeepTools, and the reads counts were normalized to RPKM.
Supplementary_files_format_and_content: *.bed: The peak files are generated by MACS2, and peaks with fold enrichment less than 2 were removed.

molecule: genomic DNA

source_name: embryo

extract_protocol:
The embryos were washed 3 times in 0.5% BSA after removal of zona pellucida. After cell lysis, the chromatin was digested with micrococcal nuclease and histone-DNA complexes were isolated with corresponding antibodies.
Libraries were prepared with NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645).

value: embryo

value: late 2-cell

tag: age

value: 36h after fertilization

value: embryos were injected with Hdac1 mutant mRNA at zygote stage

tag: chip antibody


title: MU_H3K4me3_IP1


title: Ctrl7

description:
reads_counts_RYR2
LIB034354_TRA00123609_S13

value: AAV-Cre


title: KO6

description:
reads_counts_RYR2
LIB034354_TRA00123608_S12


title: H2O_H3K4me3_IP2

value: embryos were injected with H2O at zygote stage


title: Ctrl6

description:
reads_counts_RYR2
LIB034354_TRA00123607_S11


title: NR24

city: Kiel

first: Meike

last: Kaehler

state: Schleswig-Holstein

address: Hospitalstraße 4

department: Institute of Experimental and Clinical Pharmacology

country: Germany

institute: University Hospital Schleswig-Holstein, Campus Kiel

description: Gene expression data from K-562 cells, resistant to 0.1 µM nilotinib, biological replicate 2.

hyb_protocol: 3.5 µg fragmented and labeled ssDNA were hybridized on HuGene 2.0 arrays for 16 h at 45 °C using the Affymetrix Hybridization Oven 640. The arrays were washed using the Fluidics Station 450 using the protocol FS450_0002.

scan_protocol: The arrays were scanned using the Affymetrix GeneChip Scanner.

data_processing: The data were processed using the Transcriptome Analysis Console 4.0 (Thermo Fisher Scientific).

label: biotin

label_protocol: 100 ng RNA was used for each sample with the GeneChip WT PLUS Reagent Kit according to the manufacturer's recommendations.

growth_protocol: Cells were grown in RPMI 1640 + 10% v/v FCS + 1 % v/v Pen/Strep w/o imatinib or nilotinib.

extract_protocol: RNA was isolated using miRVana microRNA isolation Kit (Thermo Fisher Scientific) according to the manufacturer's recommendation from 3 million cells.

treatment_protocol: Resistant cells were generated by step-wise increase of imatinib concentration to 0.5 and 2 µM imatinib or 0.1 µM nilotinib.

tag: resistance

value: resistant to 0.1 uM nilotinib


title: MU_H3K4me3_IP2

tag: tissue


title: KO5

description:
reads_counts_RYR2
LIB034354_TRA00123606_S10


title: H2O_H3K4me3_IP1


title: NR21


title: KO4

description:
reads_counts_RYR2
LIB034354_TRA00123605_S9


title: N3

description: Gene expression data from K-562 cells, resistant to 0.1 µM nilotinib, biological replicate 1.


title: H2O_H3K4me3_input2


title: Ctrl5

description:
reads_counts_RYR2
LIB034354_TRA00123604_S8


title: NR22


title: WT_50h rep1

city: Kongens Lyngby

first: Xiaobo

last: Li

address: Kemitorvet

institute: Technical University of Denmark

data_processing:
Basecalling online_YX BasecallVersion0.5.6.153
Sequenced reads were filtered by removing adaptor sequences and low-quality reads
Sequenced reads were mapped to reference sequence S.erythraea NRRL2338 (Genbank accession number: NC_009142) using Geneious Prime version 2019.1.3.
Genome_build: S.erythraea NRRL2338 (NC_009142)
Supplementary_files_format_and_content: tab-delimited text files include TPM values for each Sample

organism: Saccharopolyspora erythraea

source_name: S. erythraea NRRL23338, stationary phase

growth_protocol: Modified minimal liquid medium was used for liquid culture of S.erythraea. Cells were harvested at two time points, i.e. 10 h in the early exponential phase for total RNA extraction and 50 h in the stationary phase.

extract_protocol:
Total RNA was extracted with an RNAeasy Plus Mini kit (Qiagen, Germany) using glass beads to mechanically disrupting cells with a FastPrep (30 s ×4; M.P. Biomedical, USA). DNase treatment by RNase-Free DNase Set (Qiagen, Germany) aided to digest DNA in samples.
rRNA was removed with Illumina Ribo-zero rRNA removal kit(bacteria) after total RNA was collected. Fragmentation buffer was added for interrupting mRNA to short fragments. Taking these short fragments as templates, Random hexamer-primer were used to synthesize the first-strand cDNA. The second-strand cDNA was synthesized using buffer, dATPs,dGTPs,dCTPs,dUTPs, RNase H and DNA polymerase I respectively after removing dNTPs. Short fragments were purified with QiaQuick PCR extraction kit and resolved with EB buffer for end reparation and adding poly(A). After that, the short fragments were connected with sequencing adapters. Then, the UNG enzyme was used to degrade the second-strand cDNA, and the product was purified by MiniElute PCR Purification Kit before PCR amplification.

treatment_protocol: The  cells were harvested and resuspended in 2 mL RNAlaterTM solution (Invitrogen, USA).

tag: growth phase

value: stationary phase (50 h)

value: wild type


title: 2R24

description: Gene expression data from K-562 cells, resistant to 2 µM imatinib, biological replicate 2.

value: resistant to 2 uM imatinib


title: WT_10h rep2

source_name: S. erythraea NRRL23338, exponential phase

value: exponential phase (10 h)


title: N2


title: H2O_H3K4me3_input1


title: N1


title: MU_H3K4me3_input1


title: WT_50h rep2


title: MU_H3K27ac_IP2


title: Ctrl4

description:
reads_counts_RYR2
LIB034354_TRA00123603_S7


title: NR23


title: WT_10h rep1


title: N4


title: MU_H3K27ac_IP1


title: KO3

description:
reads_counts_RYR2
LIB034354_TRA00123601_S5


title: 2R23


title: 2R21


title: IM4

description: Gene expression data from K-562 cells, resistant to 2 µM imatinib, biological replicate 1.


title: RNA - Retene 50 µM - Replicate 3

city: Corvallis

first: Christopher

middle: Michael

last: Sullivan

state: OR

address: Sinnhuber Aquatic Research Laboratory, 28645 East HWY 34

department: Environmental & Molecular Toxicology

institute: Oregon State University

data_processing:
Reads were aligned to the GRCz11 genome using the Star Aligner
Reads were counted using HTSeq with an alignment valud cutoff of a10 using the gff file for the GRCz11 genome
Raw counts were normalized using DESeq2 for downstream analyses
Differential gene expression was assessed using DESeq2
Assembly: Grcz11

organism: Danio rerio

source_name: Whole zebrafish larvae

growth_protocol: Zebrafish (Tropical 5D line) embryos were dechorionated at 4 hours post fertilization (hpf), placed in 96-well plates in buffered embryo medium, statically exposed to retene or vehicle control beginning at 6 hpf, gently shaken overnight on an orbital shaker at 235 rpm, and then sampled at 48 hpf.

extract_protocol:
48 hpf larvae were pooled into groups of 9 fish each, briefly anesthetized on ice, and excess water removed. Tissue was immediately homogenized with RNAzol RT (Molecular Research Center) and 0.5mm zirconium oxide beads in a Bullet Blender tissue homogenizer (Next Advance). Homogenate was stored at -80 C until RNA isolation. Total RNA was isolated using a Direct-zol RNA MiniPrep Kit (Zymo Research) with DNAse treatment.
mRNA was purified using oligo(dT)-attached magnetic beads and fragmented. cDNA was synthesized using random hexamer-primed reverse transcription, end-repaired, 3' adenlylated and adapters were ligated to 3' ends. cDNA was PCR amplified.

treatment_protocol: Fish were exposed to retene at 8 concentrations: 0, 0.205, 0.512, 1.28, 3.2, 8, 20 and 50 µM

tag: timepoint

value: 48 hours post-fertilization

value: Retene 50 µM

tag: FISH strain

value: Tropical 5D


title: Ctrl2

description:
reads_counts_RYR2
LIB034354_TRA00123600_S4

tag: cell type

value: cardiomyocyte


title: RNA - Retene 50 µM - Replicate 4

tag: treatment


title: MU_H3K27ac_input1


title: Ctrl1

description:
reads_counts_RYR2
LIB034354_TRA00123599_S3

value: 4 week old


title: RNA - Retene 50 µM - Replicate 2


title: Ctrl3

description:
reads_counts_RYR2
LIB034354_TRA00123602_S6

value: heart


title: RNA - Retene 50 µM - Replicate 1


title: KO2

description:
reads_counts_RYR2
LIB034354_TRA00123598_S2


title: RNA - Retene 20 µM - Replicate 4

value: Retene 20 µM


title: KO1

description:
reads_counts_RYR2
LIB034354_TRA00123597_S1


title: H2O_H3K27ac_IP2

value: embryo

value: late 2-cell


title: H2O_H3K27ac_input2

value: 36h after fertilization


title: H2O_H3K27ac_input1


title: GFP5

description:
SMART-Seq3
ER_Stress_OE_reads_counts
Neonatal_ATF4_DESeq
Neonatal_DDIT3_DESeq

data_processing:
alignment by STAR to mm10
generate reads count matrix with FeatureCount
Differential expression analysis by DESeq2
Genome_build: mm10
Supplementary_files_format_and_content: ER_Stress_OE_reads_counts: reads count matrix
Supplementary_files_format_and_content: Neonatal_ATF4_DESeq: DESeq2 analysis result
Supplementary_files_format_and_content: Neonatal_DDIT3_DESeq: DESeq2 analysis result

value: AAV-GFP


title: 2R22

tag: cell line


title: MU_H3K27ac_input2

value: embryos were injected with Hdac1 mutant mRNA at zygote stage


title: RNA - Retene 20 µM - Replicate 3


title: IM3


title: IM2


title: IM1


title: 05R24

description: Gene expression data from K-562 cells, resistant to 0.5 µM imatinib, biological replicate 2.

value: resistant to 0.5 uM imatinib


title: 05R22


title: RNA - Retene 20 µM - Replicate 2


title: GFP4

description:
SMART-Seq2
ER_Stress_OE_reads_counts
Neonatal_ATF4_DESeq
Neonatal_DDIT3_DESeq


title: 05R23


title: H2O_H3K27ac_IP1

value: embryos were injected with H2O at zygote stage


title: GFP3


title: FK228_mouse_late_2cell_rep2

data_processing:
Reads were trimmed by Trimmomatic to remove the bases with low quality and adapter sequences.
The trimmed reads were align to GRCm38 or ARS-UCD1.2 by Hisat2.
The raw reads counts of all expressed genes were quantified by FeatureCounts.
The abundance of all expressed genes were normalized to FPKM by Cufflinks.
Differentially expressed genes were selected by DESeq2 with Padj. <0.05 and fold change (FC) of >2 or <0.5.
Genome_build: GRCm38, ARS-UCD1.2
Supplementary_files_format_and_content: *.txt: Tab-delimited text files include FPKM calculated by Cufflinks for each sample.

extract_protocol:
Total RNA was isolated from embryos using Picopure RNA isolation kit (Life Technologies, Grand Island, NY, USA) according to the manufacturer's instruction.
mRNAs were separated with oligo(dT)25 beads, and was used to prepare sequencing libraries with NEB Next Ultra RNA Library Prep Kit for Illumina (New England Biolabs). Briefly, mRNA was fragmented and reverse transcribed. The cDNA library was subject to end repair, poly(A)-tailing, adaptor ligation, and PCR amplification of 12–15 cycles for sequencing library construction.

value: embryos were treated with FK228 (50 nM) from zygote stage


title: 05R21


title: RNA - Retene 20 µM - Replicate 1


title: FK228_mouse_late_2cell_rep1


title: DMSO_mouse_late_2cell_rep2

value: embryos were cultured in KSOM with DMSO


title: RNA - Retene 8 µM - Replicate 4

value: Retene 8 µM


title: 05M4

description: Gene expression data from K-562 cells, resistant to 0.5 µM imatinib, biological replicate 1.


title: GFP2


title: RNA - Retene 8 µM - Replicate 2

value: 48 hours post-fertilization


title: DMSO_mouse_late_2cell_rep1


title: RNA - Retene 8 µM - Replicate 3


title: DDIT3OE4


title: DDIT3OE5


title: GFP1


title: RNA - Retene 3.2 µM - Replicate 4

value: Retene 3.2 µM


title: 05M2


title: RNA - Retene 3.2 µM - Replicate 3

value: Tropical 5D


title: Hdac2_MU_late_2cell_rep2

value: embryos were injected with Hdac2 mutant mRNA at zygote stage


title: Hdac2_MU_late_2cell_rep1


title: Hdac2_WT_late_2cell_rep1

value: embryos were injected with Hdac2 WT mRNA at zygote stage


title: DDIT3OE2

value: heart

value: cardiomyocyte

value: 4 week old


title: RNA - Retene 8 µM - Replicate 1


title: DDIT3OE3


title: RNA - Retene 3.2 µM - Replicate 2


title: 05M3


title: DDIT3OE1


title: 05M1


title: Hdac2_WT_late_2cell_rep2

value: embryo

value: late 2-cell


title: K4

description: Gene expression data from treatment-naive K-562 cells

source_name: native

value: treatment-naive


title: K3


title: Hdac1_MU_Kdm5b_late_2cell_rep2

value: 36h after fertilization

value: embryos were injected with Hdac1 mutant mRNA at zygote stage and then injected with Kdm5b mRNA into 2 blastomeres at early 2-cell stage


title: RNA - Retene 3.2 µM - Replicate 1


title: Hdac1_MU_H2O_late_2cell_rep1

value: embryos were injected with Hdac1 mutant mRNA at zygote stage and then injected with H2O into 2 blastomeres at early 2-cell stage


title: RNA - Retene 1.28 µM - Replicate 3

value: Retene 1.28 µM


title: K2


title: ATF4OE2


title: RNA - Retene 1.28 µM - Replicate 2


title: RNA - Retene 1.28 µM - Replicate 4


title: Hdac1_MU_early_2cell_rep2

value: early 2-cell

value: 24h after fertilization

value: embryos were injected with Hdac1 mutant mRNA at zygote stage


title: FK228_bovine_8/16cell_rep2

organism: Bos taurus

value: 72h after fertilization

value: embryos were treated with FK228 (50 nM) from zygote stage


title: ATF4OE5


title: Hdac1_WT_early_2cell_rep2

value: embryos were injected with Hdac1 WT mRNA at zygote stage


title: Hdac1_MU_H2O_late_2cell_rep2


title: RNA - Retene 0.512 µM - Replicate 4

value: 48 hours post-fertilization

value: Retene 0.512 µM

value: Tropical 5D


title: S3


title: ATF4OE3

value: heart

value: cardiomyocyte

value: 4 week old


title: RNA - Retene 1.28 µM - Replicate 1


title: S4

value: treatment-naive


title: Hdac1_MU_Kdm5b_late_2cell_rep1

value: embryo

value: late 2-cell

value: 36h after fertilization


title: FK228_bovine_8/16cell_rep1


title: K1


title: RNA - Retene 0.512 µM - Replicate 3


title: ATF4OE4


title: Hdac1_WT_early_2cell_rep1


title: DMSO_bovine_8/16cell_rep1

value: embryos were cultured in BO-IVC medium with DMSO


title: BN 1-LS RNAseq mTAL nz950

city: Milwaukee

first: Alison

middle: J.

last: Kriegel

state: WI

address: 8701 Watertown Plank Rd.

department: Physiology

institute: Medical College of Wisconsin

data_processing:
Adapter sequences were removed from the output RNA-Seq sequence reads using cutadapt (http://code.google.com/p/cutadapt/)
Reads with low base quality (<13) were further trimmed and, if less than 25 base pairs, removed by FastQC (http://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
The trimmed sequence reads were then aligned to the rat reference genome and gene annotation database (rn5) using TopHat2 (v2.1.1) with mammalian default parameters (PMID:23618408)
Transcripts in all of the sequencing libraries were assembled by Stringtie (PMID:25690850)
Differential expression analysis was performed by using Cufflinks (v2.2.1) (PMID: 20436464 )
The Benjamini-Hochberg procedure was used for controlling false discovery rates in multiple comparisons
Assembly: rn5
Supplementary files format and content: Transcript expression data was organized by transcript name, with FDR adjusted q-values <0.05 considered significantly differentially expressed genes for downstream pathway analysis.
Supplementary files format and content: Transcript expression analysis by isoform
Supplementary files format and content: mRNA mapping rate

organism: Rattus

growth_protocol: Rats were obtained from colonies maintained at the Medical College of Wisconsin at 7 weeks of age. Rats were maintained on 0.4% NaCl, #113755, Dyets Inc. after weaning and provided food and water ad libitum.

extract_protocol:
Kidneys were perfused with Hanks’ balanced salt solution containing 20 mM HEPES (pH=7.4, 4°C). The kidneys were excised, the inner stripe of the outer medulla dissected, cut into small pieces, and incubated in digestion buffer for microscopy-guided manual selection of mTAL.   Isolated mTAL were isolated and flash frozen in liquid nitrogen.  Total RNA was extracted using a Trizol-based method and quantified by Nanodrop. RNA deep sequencing and analysis was performed as previously described by the Human Molecular Genetic Center at the Medical College of Wisconsin (Evans LC, et al.; Physiol Genomics, 2018;  PMCID: PMC6032288).
RNA libraries were constructed on each individual animal using an Illumina TruSeq Stranded Total RNA Kit
Samples were multiplexed on a 400 GB flow cell and pair-end sequenced using an Illumina HiSeq 2500.

treatment_protocol: No other treatment was administered.

value: SS-Chr 1BN/Mcwi

value: male

tag: diet

value: isolated medullary thick ascending limb (mTAL) cells


title: Hdac1_MU_early_2cell_rep1

value: early 2-cell

value: 24h after fertilization

value: embryos were injected with Hdac1 mutant mRNA at zygote stage


title: BN 1-LS RNAseq mTAL nz951

source_name: isolated medullary thick ascending limb (mTAL)


title: RNA - Retene 0.512 µM - Replicate 2

value: 48 hours post-fertilization


title: Hdac1_MU_late_2cell_rep2


title: S2


title: BN 1-LS RNAseq mTAL nz949


title: RNA - Retene 0.512 µM - Replicate 1

value: Retene 0.512 µM


title: SS -LS RNAseq mTAL nz948

value: SS/JrHsdMcwi


title: Hdac1_MU_late_2cell_rep1


title: KPPC;Col1pdxKO PDAC cell line 7-18-6

city: Houston

first: Yang

last: Chen

state: Texas

address: 1881 East Road

department: Cancer Biology

institute: UT-MD Anderson Cancer Center

data_processing:
Fastq files from the Illumina platform were aligned to the reference genome mm10 using the Spliced Transcripts Alignment to a Reference (STAR) algorithm
HTSeq-count was then utilized to generate the raw counts for each gene
Raw counts were then analyzed by DESeq2 for data processing, normalization, and differential expression analysis according to standard procedures.
Genome_build: Raw sequencing data from the Illumina platform were converted into Fastq files and aligned to the reference genome mm10 using the Spliced Transcripts Alignment to a Reference (STAR) algorithm
Supplementary_files_format_and_content: txt

source_name: Mouse pancreatic cancer cell total RNA

growth_protocol: Cancer cells cultured in DMEM with 1%FBS and 1% PS on pre-coated plates

extract_protocol:
Direct-zol RNA extraction kit (Zymo)
Illumina TrueSeq stranded mRNAseq Library

treatment_protocol: Cancer cells cultured in 6-well plates pre-coated with vehicle, collagen 1 homotrimers, or collagen 1 heterotrimers

tag: detailed genotype

value: Coated Collagen 1 heterotrimers on plates


title: DMSO_bovine_8/16cell_rep2

value: 72h after fertilization


title: Hdac1_WT_late_2cell_rep2

value: embryos were injected with Hdac1 WT mRNA at zygote stage


title: KPPC;Col1pdxKO PDAC cell line 7-18-5


title: RNA - Retene 0.205 µM - Replicate 4

value: Retene 0.205 µM

value: Tropical 5D


title: S1

value: treatment-naive


title: RNA - Retene 0.205 µM - Replicate 3


title: SS -LS RNAseq mTAL nz946


title: SS -LS RNAseq mTAL nz947


title: KPPC;Col1pdxKO PDAC cell line 7-18-4


title: BN 1-LS RNAseq mTAL nz945


title: H2O_late_2cell_rep2

value: embryo

value: late 2-cell

value: 36h after fertilization

value: embryos were injected with H2O at zygote stage


title: Hdac1_WT_late_2cell_rep1


title: KPPC;Col1pdxKO PDAC cell line 7-18-3

value: Coated Collagen 1 homotrimers on plates


title: RNA - Retene 0.205 µM - Replicate 2


title: H2O_late_2cell_rep1


title: BN 1-LS RNAseq mTAL nz944

value: SS-Chr 1BN/Mcwi

value: male

value: isolated medullary thick ascending limb (mTAL) cells


title: RNA - Vehicle control - Replicate 4

value: 48 hours post-fertilization

value: DMSO Vehicle control


title: RNA - Retene 0.205 µM - Replicate 1


title: KPPC;Col1pdxKO PDAC cell line 7-18-9

value: Coated vehicle on plates


title: input2

data_processing:
aligned to mm10 using Bowtie2
Peaks were called with MACS2 against input chromatin background
DeepTools was used to generate aggregation and heatmap plots

source_name: heart ventricle

extract_protocol:
A microtip sonicator (QSONICA Q700) was used at 60% amplitude and a cycle of 5 sec on and 20 sec off for 96 cycles in total. Sheared chromatin was precleared by incubation with 100 µl Dynabeads Protein A (Life Technologies, 10002D) for 1 hour at 4C. The precleaned chromatin was then incubated with 100 µl Dynabeads M-280 Streptavidin (Life Technologies, 11206D) for 1 hour at 4C. The streptavidin beads were washed and bound DNA eluted. BioChIP DNA was purified with MinElute PCR Purification kit (Qiagen, 28006).
KAPA Biosystems KK8500

treatment_protocol: The tissues were minced in 1% formaldehyde-containing PBS at room temperature by a motor-driven homogenizer (T10 basic. IKA). The tissue suspension was shaken for 15 min at room temperature for crosslinking. Next, Glycine was added at a final concentration of 125 mM to quench formaldehyde.

value: 4 weeks

value: heart ventricle

tag: antibody


title: BN 1-LS RNAseq mTAL nz943

tag: genotype


title: RNA - Vehicle control - Replicate 3


title: KPPC;Col1pdxKO PDAC cell line 7-18-8


title: SS -LS RNAseq mTAL nz942

value: SS/JrHsdMcwi

tag: Sex


title: RNA - Vehicle control - Replicate 2


title: KPPC;Col1pdxKO PDAC cell line 7-18-7


title: SS -LS RNAseq mTAL nz941


title: ATF4-bio2

value: AAV


title: ATF4-bio1


title: RNA - Vehicle control - Replicate 1

value: Tropical 5D


title: KPPC;Col1pdxKO PDAC cell line 7-18-2


title: SS -LS RNAseq mTAL nz940


title: genomic DNA from stroke patients Replication 62

status: Public on May 20 2022

type: genomic

city: terrassa

first: NATALIA

last: CULLELL

address: sant antoni 19

country: Spain

institute: fundació mutuaterrassa

description: DNA from whole blood from stroke patients

hyb_protocol: bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 and EPIC Beadchip using standard Illumina protocol

scan_protocol: Arrays were imaged using GenomeStudio using standard recommended Illumina scanner setting

data_processing:
GenomeStudio software
normalized (Noob) Average Beta

label: Cy3 and Cy5

source_name: stroke patients first 24 h 62

label_protocol: Standard Illumina Protocol

extract_protocol: Genomic DNA was extracted using the Gentra Puregene Blood Kit (Quiagen, Hilden, Germany)

tag: gender

value: M

tag: delta-nihss


title: genomic DNA from stroke patients Replication 61

source_name: stroke patients first 24 h 61

tag: age


title: genomic DNA from stroke patients Replication 59

source_name: stroke patients first 24 h 59


title: genomic DNA from stroke patients Replication 57

source_name: stroke patients first 24 h 57

value: F


title: genomic DNA from stroke patients Replication 56

source_name: stroke patients first 24 h 56


title: genomic DNA from stroke patients Replication 60

source_name: stroke patients first 24 h 60


title: KPPC;Col1pdxKO PDAC cell line 7-18-1


title: genomic DNA from stroke patients Replication 58

source_name: stroke patients first 24 h 58


title: genomic DNA from stroke patients Replication 55

source_name: stroke patients first 24 h 55


title: genomic DNA from stroke patients Replication 54

source_name: stroke patients first 24 h 54


title: genomic DNA from stroke patients Replication 53

source_name: stroke patients first 24 h 53


title: input1


title: genomic DNA from stroke patients Replication 50

source_name: stroke patients first 24 h 50


title: genomic DNA from stroke patients Replication 51

source_name: stroke patients first 24 h 51


title: genomic DNA from stroke patients Replication 47

source_name: stroke patients first 24 h 47


title: genomic DNA from stroke patients Replication 46

source_name: stroke patients first 24 h 46


title: genomic DNA from stroke patients Replication 49

source_name: stroke patients first 24 h 49


title: genomic DNA from stroke patients Replication 45

source_name: stroke patients first 24 h 45


title: genomic DNA from stroke patients Replication 42

source_name: stroke patients first 24 h 42


title: genomic DNA from stroke patients Replication 44

source_name: stroke patients first 24 h 44


title: genomic DNA from stroke patients Replication 41

source_name: stroke patients first 24 h 41


title: genomic DNA from stroke patients Replication 43

source_name: stroke patients first 24 h 43


title: genomic DNA from stroke patients Replication 38

source_name: stroke patients first 24 h 38


title: genomic DNA from stroke patients Replication 39

source_name: stroke patients first 24 h 39


title: genomic DNA from stroke patients Replication 37

source_name: stroke patients first 24 h 37


title: genomic DNA from stroke patients Replication 40

source_name: stroke patients first 24 h 40


title: genomic DNA from stroke patients Replication 35

source_name: stroke patients first 24 h 35


title: genomic DNA from stroke patients Replication 33

source_name: stroke patients first 24 h 33


title: genomic DNA from stroke patients Replication 32

source_name: stroke patients first 24 h 32


title: genomic DNA from stroke patients Replication 36

source_name: stroke patients first 24 h 36


title: genomic DNA from stroke patients Replication 34

source_name: stroke patients first 24 h 34


title: genomic DNA from stroke patients Replication 28

source_name: stroke patients first 24 h 28


title: genomic DNA from stroke patients Replication 31

source_name: stroke patients first 24 h 31


title: genomic DNA from stroke patients Replication 29

source_name: stroke patients first 24 h 29


title: genomic DNA from stroke patients Replication 26

source_name: stroke patients first 24 h 26


title: genomic DNA from stroke patients Replication 25

source_name: stroke patients first 24 h 25


title: genomic DNA from stroke patients Replication 52

source_name: stroke patients first 24 h 52


title: genomic DNA from stroke patients Replication 27

source_name: stroke patients first 24 h 27


title: genomic DNA from stroke patients Replication 24

source_name: stroke patients first 24 h 24


title: genomic DNA from stroke patients Replication 48

source_name: stroke patients first 24 h 48


title: genomic DNA from stroke patients Replication 23

source_name: stroke patients first 24 h 23


title: genomic DNA from stroke patients Replication 20

source_name: stroke patients first 24 h 20


title: genomic DNA from stroke patients Replication 18

source_name: stroke patients first 24 h 18


title: genomic DNA from stroke patients Replication 22

source_name: stroke patients first 24 h 22


title: genomic DNA from stroke patients Replication 21

source_name: stroke patients first 24 h 21


title: genomic DNA from stroke patients Replication 15

source_name: stroke patients first 24 h 15


title: genomic DNA from stroke patients Replication 19

source_name: stroke patients first 24 h 19


title: genomic DNA from stroke patients Replication 13

source_name: stroke patients first 24 h 13


title: genomic DNA from stroke patients Replication 12

source_name: stroke patients first 24 h 12


title: genomic DNA from stroke patients Replication 30

source_name: stroke patients first 24 h 30


title: genomic DNA from stroke patients Replication 17

source_name: stroke patients first 24 h 17


title: genomic DNA from stroke patients Replication 8

source_name: stroke patients first 24 h 8


title: genomic DNA from stroke patients Replication 14

source_name: stroke patients first 24 h 14


title: genomic DNA from stroke patients Replication 1

source_name: stroke patients first 24 h 1


title: genomic DNA from stroke patients Replication 16

source_name: stroke patients first 24 h 16


title: genomic DNA from stroke patients Replication 5

source_name: stroke patients first 24 h 5


title: genomic DNA from stroke patients Replication 2

source_name: stroke patients first 24 h 2


title: genomic DNA from stroke patients Replication 11

source_name: stroke patients first 24 h 11


title: genomic DNA from stroke patients Replication 10

source_name: stroke patients first 24 h 10


title: genomic DNA from stroke patients Replication 9

source_name: stroke patients first 24 h 9


title: 9hour_with_mero_2

city: Richardson

first: Joseph

last: Boll

state: TX

address: 800 W Campbell

department: Biological Sciences

institute: University of Texas at Dallas

data_processing:
CLC genomic workbench software (Qiagen) was used to process reads
Reads were aligned  to the A. baumannii ATCC 17978 genome annotations lue.
The RPKM expression values and the weighted proportions fold change of expression values between meropenem treated and treated samples at the same timepoint were calcualted.
Baggerley’s test on proportions was used to generate a false discovery rate adjusted P-value.
Genome_build: GCA_000015425.1
Supplementary_files_format_and_content: tab-delimited text files include RPKM values for each Sample ...
Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every sample

organism: Acinetobacter baumannii

growth_protocol: An overnight culture of A. baumannii strain ATCC 17978 was backdiluted 1:10 in fresh, pre-warmed BH broth containing meropenem or equivalent volume of water. Cultres were incubated at 37 degress for 0.5, 3, and 9 h in triplicate.

extract_protocol:
Direct-Zol RNA MiniPrep kit (Zymo Research) was used to extract total RNA from cultures. Turbo DNA-free DNA removal kit (Invitrogen) was used to remove genomic DNA contamination.
Done at MiGS - rRNA depletion was done using RiboZero Plus. Libraries were prepared using stranded library prep and 12 M paired end reads were obtained for each sample using Illumina NextSeq 550..

treatment_protocol: Cultures were washed and stored in RNAlater (sigma).

tag: strain

value: meropenem treated

tag: time point

value: 9 hours


title: 9hour_no_mero_3

value: untreated


title: genomic DNA from stroke patients Replication 4

source_name: stroke patients first 24 h 4


title: genomic DNA from stroke patients Replication 6

source_name: stroke patients first 24 h 6


title: genomic DNA from stroke patients Replication 7

source_name: stroke patients first 24 h 7


title: 3h_mero_1

value: 3 hours


title: 30min_mero_3

value: 30 minutes


title: 9hour_with_mero_3


title: genomic DNA from stroke patients Replication 3

source_name: stroke patients first 24 h 3


title: 9hour_with_mero_1


title: 9hour_no_mero_2


title: 30min_no_mero_3


title: 30min_no_mero_2


title: 3h_mero_3


title: 9hour_no_mero_1


title: 3h_mero_2


title: 3h_no_mero_3


title: 3h_no_mero_1


title: 3h_no_mero_2


title: 30min_mero_2


title: 30min_mero_1


title: OSANDD

city: Stanford

first: Cheen Euong

last: Ang

address: 265 Campus Drive, G3145

institute: Stanford University

description: OSANDD

data_processing:
Reads were mapped with Tophat
FPKM was obtained with Cufflinks with default settings.
Assembly: hg19
Supplementary files format and content: processed file contains FPKM values

source_name: Induced oligodendrocyte precursor cells with [Olig2, Sox10, Ascl1, Nkx2.2, p53DD]

growth_protocol: human dermal fibroblasts were cultured in Dulbecco’s modified Eagle medium (DMEM) containing 10% fetal bovine serum and 1% Penicillin-Streptomycin.

extract_protocol:
Total RNA was harvested using Trizol
libraries were built using Truseq kit with ribosomal RNA depletion (Illumina).

treatment_protocol: for induction of oligodendrocyte precursor cells, retroviruses expressing the combination of transcription factors mixed with polybrene (4 μg /ml) (Sigma) and incubated under centrifugation (700RCF, 1h, RT) for spinfection as described in a previous paper3. The medium was replaced with fresh medium after spinfection. Three days later, medium was changed to PDGF medium containing DMEM/F12, N2 (1X), B27 (1X), Penicillin-Streptomycin (1X), NEAA (1X), Insulin (25ug/ml, Sigma), PDGF-AA 10ng/ml, Peprotech), IGF (10ng/ml, Peprotech), NT3 (1ng/ml, Peprotech), Biotin (100ng/ml, Sigma), and cAMP (1uM, Sigma). Fifty percent of PDGF medium was changed every other day until day 21.

value: Induced oligodendrocyte precursor cells


title: 120-D0

source_name: Human dermal fibroblast line 120

value: dermal fibroblast


title: 30min_no_mero_1


title: ATF4OE1

source_name: heart

tag: tissue

value: heart

value: cardiomyocyte

tag: developmental stage

value: 4 week old


title: 1419-D0

source_name: Human dermal fibroblast line 1419


title: 5-hOPC-pool

description: hOPC-pool

source_name: Human primary oligodendrocyte precursor cells, pooled

value: primary oligodendrocyte precursor cells


title: OSAN2

source_name: Induced oligodendrocyte precursor cells with [Olig2, Sox10, Ascl1, Nkx2.2]


title: Mock

description: Mock

source_name: Mock control (human dermal fibroblasts infected with GFP virus)


title: 119-D0

source_name: Human dermal fibroblast line 119


title: iPS-OPC-pool

description: iPS-OPC-pool

source_name: human iPS cell-derived oligodendrocyte precursor cells, pooled


title: 6-hOPC-pool

description: hOPC-pool


title: 6-3_02032017

city: Boston

first: Ionita

middle: Calin

last: Ghiran

address: 3 Blackfan Circle

department: Medicine

institute: Beth Israel Deaconess Medical Center

description: total cell-free biofluid RNA

data_processing:
All FASTQ files were processed uniformly through the extra-cellular RNA processing toolkit (exceRpt), with an adapter sequence of [] and random barcode length [] (on []). Information about exceRpt is available at https://github.gersteinlab.org/exceRpt/.
Assembly: hg19
Supplementary files format and content: Each processed data file is a core results archive generated by exceRpt. The core results archive contains read counts for alignments to the different transcriptome libraries used by exceRpt (miRNA / piRNA / tRNA / GENCODE annotations / circRNA), taxonomy trees for exogenous rRNA and genomic reads aligned by exceRpt, and a variety of other files generated by exceRpt.

source_name: Plasma

extract_protocol:
RNA Isolation method was . RNA Isolation kit was miRcury biofluids (Exiqon).
Library construction kit was NEBNext small RNA library prep (NEB). Samples were not multiplexed. Type of Starting Material was total RNA.

tag: disease

value: Healthy Subject

tag: time of collection

tag: anatomical location

value: Entire cardiovascular system


title: 3-3_02032017

description: total cell-free biofluid RNA


title: 5-2_02032017

description: total cell-free biofluid RNA


title: 4-1_02032017

description: total cell-free biofluid RNA


title: 4-2_02032017

description: total cell-free biofluid RNA


title: 4-3_02032017

description: total cell-free biofluid RNA


title: 3-2_02032017

description: total cell-free biofluid RNA


title: 7-2_02032017

description: total cell-free biofluid RNA


title: OSAN2-6

source_name: Induced oligodendrocyte precursor cells with [Olig2, Sox10, Ascl1, Nkx2.2, Nkx 6.1]

tag: cell type


title: 6-1_02032017

description: total cell-free biofluid RNA


title: 8-2_02032017

description: total cell-free biofluid RNA


title: 5-3_02032017

description: total cell-free biofluid RNA


title: 1-1_02032017

description: total cell-free biofluid RNA


title: 1-3_02032017

description: total cell-free biofluid RNA


title: 7-1_02032017

description: total cell-free biofluid RNA


title: 6-2_02032017

description: total cell-free biofluid RNA


title: 2-2_02032017

description: total cell-free biofluid RNA


title: 3-1_02032017

description: total cell-free biofluid RNA


title: 2-1_02032017

description: total cell-free biofluid RNA


title: 1-2_02032017

description: total cell-free biofluid RNA


title: 5-1_02032017

description: total cell-free biofluid RNA


title: 7-3_02032017

description: total cell-free biofluid RNA


title: human HGG, Input2

first: Debosmita

last: Sardar

address: 1 Baylor Plaza

institute: Baylor College of Medicine

data_processing:
Quality control was performed using fastQC (v0.11.17) and MultiQC (v0.9).
Reads were mapped to the mouse genome mm10 or human genome hg38 assembly using bowtie2
Using the HOMER software suite, bedgraph files and tag directories were made
findPeaks command in histone (for H3K27ac) or factor (for Sox9) mode was used to filter ChIP peaks enriched over Input control
Annotation of enriched peaks was performed using annotatePeaks with mm10 or hg38 assembly
Assembly: Integrated Genome Browser compatible files were made using samtools, sort and index, deepTools bamCompare
Supplementary files format and content: mm10; hg38
Supplementary files format and content: bw, narrowPeak

source_name: human HGG tumor tissue

extract_protocol:
Tissue was dissociated, crosslinked with 1.1% formaldehyde for 10 min, quenched and frozen. After thawing, cells were lysed to release nuclei, followed by nuclear lysis. Lysates were sonicated using a Diagenode Bioruptor.
ChIP-Seq libraries were prepared using the TruSeq ChIP Library Preparation Kit (Ilumina, IP-202-1012). 10 ng of DNA was used as starting material for Input and IP samples. Libraries ranging from 250-350 bp were extracted from agarose gel incisions and PCR amplified for 18 cycles. Libraries were purified using AMPure XP beads and library quality was validated using a Standard Sensitivity NGS Fragment Analysis Kit.

value: human HGG tumor tissue

value: control

value: HGG control


title: human HGG, ChIP-H3K27ac


title: 8-3_02032017

description: total cell-free biofluid RNA


title: human HGG, ChIP-Sox9


title: 2-3_02032017

description: total cell-free biofluid RNA


title: 15_3g_UtrNK_A225_C9_S225 : SS2 sample

city: Oxford

first: Gurman

last: Kaur

address: Headley Way

department: John Radcliffe Hospital

country: United Kingdom

institute: University of Oxford

description: SmartSeq2 data for each individual cell with the listed Sample ID

library_source: transcriptomic single cell

data_processing:
RNA-Seq: bcl2fastq v.2.17.1.14, RSEM v.1.2.8
scRNA-Seq: CellRanger v2.1.0 (mkref and mkfastq), CellRanger v2.0.1 (count)
Supplementary files format and content: H5, .csv, .tsv

source_name: mouse maternal-fetal interface

extract_protocol:
Isolation of cells from the implantation sites were done with enzymatic digestion using Liberase. For SMART-Seq2 processing, single cells were FACS sorted into 96 U-well plates into either trNK (NCR1+CD3ε-TCRb-CD19-CD45+CD122+CD49a+) or cNK (NCR1+CD3ε-TCRb-CD19-CD45+CD122+CD49b+) cells. For droplet-based scRNA-seq, total uNK cells (NCR1+CD3ε-TCRb-CD19-CD45+CD122+) were sorted into Eppendorf tubes.
RNA-Seq: SMART-Seq2 scRNA-seq libraries were prepared from trNK and cNK cells from gd9.5 maternal-fetal interface and sorted in 96 U-well plates. Libraries were prepared using Nextera XT DNA library preparation kit and sequenced using a NextSeq 500/550 High Output v2 kit 75 cycles (Illumina) on a Nextseq 500 (Illumina).
scRNA-Seq: For droplet-based scRNA-seq, 7,000 cells (sorted uNK or total unsorted cells) were loaded onto the Chromium Single Cell 3’ Chip A (10x Genomics) and processed through the chromium controller to generate Gel beads in Emulsion. RNA-seq libraries were prepared using the Chromium Single Cell 3’ Library & Gel Bead Kit v2 (10x Genomics) as per the manufacturer’s instructions. Libraries were sequenced on an Illumina Hi-Seq.

value: mouse maternal-fetal interface

tag: sample id

tag: mouse id

tag: technology

value: full length (SS2) scRNa-Seq

tag: atlas

value: maternal-fetal NK interface atlas (SS2)


title: 11_10E_UtrNK_A131_C11_S131 : SS2 sample

tag: genotype

value: filtered out of SS2 NK atlas


title: 15_3g_UtrNK_A280_H4_S280 : SS2 sample


title: 11_10E_UtrNK_A154_E10_S154 : SS2 sample


title: 15_3g_UtrNK_A271_G7_S271 : SS2 sample


title: 11_10E_UcNK_A357_F9_S357 : SS2 sample


title: 11_10E_UtrNK_A181_H1_S181 : SS2 sample


title: 11_10E_UcNK_A337_E1_S337 : SS2 sample


title: 15_3g_UcNK_A58_E10_S58 : SS2 sample


title: 15_3g_UcNK_A91_H7_S91 : SS2 sample


title: 15_3g_UtrNK_A211_B7_S211 : SS2 sample


title: 15_3g_UtrNK_A205_B1_S205 : SS2 sample


title: 11_10E_UtrNK_A150_E6_S150 : SS2 sample


title: 15_3g_UtrNK_A212_B8_S212 : SS2 sample


title: 11_10E_UtrNK_A152_E8_S152 : SS2 sample


title: 11_10E_UcNK_A371_G11_S371 : SS2 sample


title: human HGG, Input1


title: 15_3g_UtrNK_A224_C8_S224 : SS2 sample


title: 11_10E_UcNK_A311_B11_S311 : SS2 sample


title: 11_10E_UtrNK_A155_E11_S155 : SS2 sample


title: 11_10E_UcNK_A332_D8_S332 : SS2 sample


title: 15_3g_UtrNK_A214_B10_S214 : SS2 sample


title: 15_3g_UtrNK_A255_F3_S255 : SS2 sample


title: 11_10E_UtrNK_A110_B2_S110 : SS2 sample


title: 15_3g_UtrNK_A197_A5_S197 : SS2 sample


title: 15_3g_UtrNK_A270_G6_S270 : SS2 sample


title: 15_3g_UtrNK_A221_C5_S221 : SS2 sample


title: 15_3g_UcNK_A15_B3_S15 : SS2 sample


title: 11_10E_UtrNK_A177_G9_S177 : SS2 sample


title: 11_10E_UtrNK_A101_A5_S101 : SS2 sample


title: 11_10E_UtrNK_A184_H4_S184 : SS2 sample


title: 11_10E_UcNK_A342_E6_S342 : SS2 sample


title: 15_3g_UcNK_A75_G3_S75 : SS2 sample


title: 15_3g_UcNK_A19_B7_S19 : SS2 sample


title: 11_10E_UtrNK_A98_A2_S98 : SS2 sample


title: 11_10E_UcNK_A305_B5_S305 : SS2 sample


title: 11_10E_UcNK_A323_C11_S323 : SS2 sample


title: 15_3g_UtrNK_A262_F10_S262 : SS2 sample


title: 11_10E_UcNK_A335_D11_S335 : SS2 sample


title: 11_10E_UcNK_A325_D1_S325 : SS2 sample


title: 11_10E_UtrNK_A190_H10_S190 : SS2 sample


title: 15_3g_UcNK_A6_A6_S6 : SS2 sample


title: 15_3g_UcNK_A71_F11_S71 : SS2 sample


title: 11_10E_UtrNK_A129_C9_S129 : SS2 sample


title: 15_3g_UtrNK_A198_A6_S198 : SS2 sample


title: 15_3g_UcNK_A65_F5_S65 : SS2 sample


title: 11_10E_UcNK_A338_E2_S338 : SS2 sample


title: 15_3g_UcNK_A96_H12_S96 : SS2 sample


title: 11_10E_UcNK_A373_H1_S373 : SS2 sample


title: 15_3g_UcNK_A83_G11_S83 : SS2 sample


title: 11_10E_UcNK_A346_E10_S346 : SS2 sample


title: 15_3g_UtrNK_A204_A12_S204 : SS2 sample


title: 11_10E_UcNK_A344_E8_S344 : SS2 sample


title: 11_10E_UcNK_A352_F4_S352 : SS2 sample


title: 15_3g_UcNK_A45_D9_S45 : SS2 sample


title: 11_10E_UcNK_A378_H6_S378 : SS2 sample


title: 11_10E_UcNK_A308_B8_S308 : SS2 sample


title: 11_10E_UcNK_A301_B1_S301 : SS2 sample


title: 11_10E_UcNK_A324_C12_S324 : SS2 sample


title: 11_10E_UcNK_A300_A12_S300 : SS2 sample


title: 11_10E_UtrNK_A111_B3_S111 : SS2 sample


title: 11_10E_UcNK_A290_A2_S290 : SS2 sample


title: 11_10E_UtrNK_A116_B8_S116 : SS2 sample


title: 15_3g_UcNK_A74_G2_S74 : SS2 sample


title: 11_10E_UcNK_A365_G5_S365 : SS2 sample


title: 15_3g_UtrNK_A219_C3_S219 : SS2 sample


title: 15_3g_UtrNK_A249_E9_S249 : SS2 sample


title: 11_10E_UcNK_A347_E11_S347 : SS2 sample


title: 15_3g_UtrNK_A240_D12_S240 : SS2 sample


title: 15_3g_UtrNK_A256_F4_S256 : SS2 sample


title: 15_3g_UcNK_A1_A1_S1 : SS2 sample


title: 15_3g_UcNK_A57_E9_S57 : SS2 sample


title: 15_3g_UtrNK_A278_H2_S278 : SS2 sample


title: 11_10E_UcNK_A329_D5_S329 : SS2 sample


title: 11_10E_UtrNK_A143_D11_S143 : SS2 sample


title: 11_10E_UcNK_A302_B2_S302 : SS2 sample


title: 15_3g_UtrNK_A201_A9_S201 : SS2 sample


title: 15_3g_UcNK_A60_E12_S60 : SS2 sample


title: 15_3g_UcNK_A56_E8_S56 : SS2 sample


title: 15_3g_UtrNK_A279_H3_S279 : SS2 sample


title: 15_3g_UtrNK_A288_H12_S288 : SS2 sample


title: 11_10E_UtrNK_A124_C4_S124 : SS2 sample


title: 11_10E_UtrNK_A112_B4_S112 : SS2 sample


title: 11_10E_UtrNK_A144_D12_S144 : SS2 sample


title: 11_10E_UcNK_A350_F2_S350 : SS2 sample


title: 15_3g_UtrNK_A273_G9_S273 : SS2 sample


title: 11_10E_UcNK_A328_D4_S328 : SS2 sample


title: 11_10E_UcNK_A345_E9_S345 : SS2 sample


title: 15_3g_UtrNK_A216_B12_S216 : SS2 sample


title: 15_3g_UtrNK_A233_D5_S233 : SS2 sample


title: 15_3g_UtrNK_A237_D9_S237 : SS2 sample


title: 15_3g_UcNK_A63_F3_S63 : SS2 sample


title: 11_10E_UtrNK_A166_F10_S166 : SS2 sample


title: 11_10E_UtrNK_A120_B12_S120 : SS2 sample


title: 11_10E_UcNK_A363_G3_S363 : SS2 sample


title: 11_10E_UtrNK_A187_H7_S187 : SS2 sample


title: 15_3g_UcNK_A54_E6_S54 : SS2 sample


title: 15_3g_UtrNK_A231_D3_S231 : SS2 sample


title: 15_3g_UtrNK_A234_D6_S234 : SS2 sample


title: 15_3g_UtrNK_A257_F5_S257 : SS2 sample


title: 15_3g_UtrNK_A266_G2_S266 : SS2 sample


title: 11_10E_UtrNK_A121_C1_S121 : SS2 sample


title: 15_3g_UtrNK_A281_H5_S281 : SS2 sample


title: 15_3g_UtrNK_A253_F1_S253 : SS2 sample


title: 15_3g_UtrNK_A230_D2_S230 : SS2 sample


title: 11_10E_UtrNK_A163_F7_S163 : SS2 sample


title: 15_3g_UcNK_A41_D5_S41 : SS2 sample


title: 11_10E_UtrNK_A148_E4_S148 : SS2 sample


title: 15_3g_UtrNK_A228_C12_S228 : SS2 sample


title: 11_10E_UtrNK_A100_A4_S100 : SS2 sample


title: 15_3g_UcNK_A39_D3_S39 : SS2 sample


title: 15_3g_UcNK_A78_G6_S78 : SS2 sample


title: 15_3g_UtrNK_A217_C1_S217 : SS2 sample


title: 15_3g_UcNK_A16_B4_S16 : SS2 sample


title: 11_10E_UtrNK_A167_F11_S167 : SS2 sample


title: 15_3g_UcNK_A72_F12_S72 : SS2 sample


title: 11_10E_UcNK_A316_C4_S316 : SS2 sample


title: 15_3g_UcNK_A27_C3_S27 : SS2 sample


title: 15_3g_UcNK_A55_E7_S55 : SS2 sample


title: 11_10E_UcNK_A362_G2_S362 : SS2 sample


title: 11_10E_UcNK_A297_A9_S297 : SS2 sample


title: 11_10E_UcNK_A355_F7_S355 : SS2 sample


title: 15_3g_UtrNK_A236_D8_S236 : SS2 sample


title: 11_10E_UcNK_A358_F10_S358 : SS2 sample


title: 15_3g_UcNK_A3_A3_S3 : SS2 sample


title: 11_10E_UtrNK_A141_D9_S141 : SS2 sample


title: 15_3g_UtrNK_A232_D4_S232 : SS2 sample


title: 15_3g_UcNK_A5_A5_S5 : SS2 sample


title: 11_10E_UcNK_A375_H3_S375 : SS2 sample


title: 11_10E_UcNK_A343_E7_S343 : SS2 sample


title: 11_10E_UcNK_A318_C6_S318 : SS2 sample


title: 15_3g_UtrNK_A282_H6_S282 : SS2 sample


title: 15_3g_UcNK_A31_C7_S31 : SS2 sample


title: 15_3g_UcNK_A82_G10_S82 : SS2 sample


title: 15_3g_UcNK_A80_G8_S80 : SS2 sample


title: 15_3g_UcNK_A87_H3_S87 : SS2 sample


title: 11_10E_UcNK_A380_H8_S380 : SS2 sample


title: 11_10E_UtrNK_A134_D2_S134 : SS2 sample


title: 15_3g_UtrNK_A220_C4_S220 : SS2 sample


title: 15_3g_UcNK_A28_C4_S28 : SS2 sample


title: 15_3g_UcNK_A85_H1_S85 : SS2 sample


title: 11_10E_UcNK_A294_A6_S294 : SS2 sample


title: 11_10E_UcNK_A367_G7_S367 : SS2 sample


title: 11_10E_UtrNK_A171_G3_S171 : SS2 sample


title: 11_10E_UtrNK_A151_E7_S151 : SS2 sample


title: 15_3g_UtrNK_A284_H8_S284 : SS2 sample


title: 15_3g_UtrNK_A194_A2_S194 : SS2 sample


title: 11_10E_UcNK_A320_C8_S320 : SS2 sample


title: 11_10E_UtrNK_A135_D3_S135 : SS2 sample


title: 11_10E_UcNK_A349_F1_S349 : SS2 sample


title: 11_10E_UtrNK_A183_H3_S183 : SS2 sample


title: 11_10E_UtrNK_A107_A11_S107 : SS2 sample


title: 15_3g_UtrNK_A242_E2_S242 : SS2 sample


title: 11_10E_UtrNK_A158_F2_S158 : SS2 sample


title: 15_3g_UcNK_A70_F10_S70 : SS2 sample


title: 11_10E_UcNK_A321_C9_S321 : SS2 sample


title: 15_3g_UtrNK_A241_E1_S241 : SS2 sample


title: 11_10E_UcNK_A336_D12_S336 : SS2 sample


title: 15_3g_UcNK_A51_E3_S51 : SS2 sample


title: 11_10E_UtrNK_A156_E12_S156 : SS2 sample


title: 15_3g_UtrNK_A209_B5_S209 : SS2 sample


title: 11_10E_UtrNK_A125_C5_S125 : SS2 sample


title: 11_10E_UcNK_A354_F6_S354 : SS2 sample


title: 15_3g_UcNK_A40_D4_S40 : SS2 sample


title: 15_3g_UtrNK_A261_F9_S261 : SS2 sample


title: 11_10E_UcNK_A307_B7_S307 : SS2 sample


title: 11_10E_UtrNK_A108_A12_S108 : SS2 sample


title: 15_3g_UtrNK_A202_A10_S202 : SS2 sample


title: 15_3g_UtrNK_A207_B3_S207 : SS2 sample


title: 11_10E_UcNK_A334_D10_S334 : SS2 sample


title: 11_10E_UtrNK_A97_A1_S97 : SS2 sample


title: 15_3g_UcNK_A46_D10_S46 : SS2 sample


title: 15_3g_UcNK_A47_D11_S47 : SS2 sample


title: 11_10E_UtrNK_A169_G1_S169 : SS2 sample


title: 15_3g_UtrNK_A259_F7_S259 : SS2 sample


title: 11_10E_UtrNK_A173_G5_S173 : SS2 sample


title: 15_3g_UcNK_A25_C1_S25 : SS2 sample


title: 11_10E_UcNK_A360_F12_S360 : SS2 sample


title: 15_3g_UtrNK_A247_E7_S247 : SS2 sample


title: 11_10E_UtrNK_A117_B9_S117 : SS2 sample


title: 11_10E_UtrNK_A147_E3_S147 : SS2 sample


title: 15_3g_UtrNK_A199_A7_S199 : SS2 sample


title: 11_10E_UtrNK_A182_H2_S182 : SS2 sample


title: 15_3g_UcNK_A13_B1_S13 : SS2 sample


title: 15_3g_UtrNK_A227_C11_S227 : SS2 sample


title: 15_3g_UtrNK_A277_H1_S277 : SS2 sample


title: 11_10E_UcNK_A327_D3_S327 : SS2 sample


title: 15_3g_UcNK_A81_G9_S81 : SS2 sample


title: 11_10E_UtrNK_A126_C6_S126 : SS2 sample


title: 15_3g_UcNK_A14_B2_S14 : SS2 sample


title: 15_3g_UtrNK_A244_E4_S244 : SS2 sample


title: 15_3g_UtrNK_A222_C6_S222 : SS2 sample


title: 11_10E_UtrNK_A109_B1_S109 : SS2 sample


title: 11_10E_UtrNK_A153_E9_S153 : SS2 sample


title: 11_10E_UtrNK_A186_H6_S186 : SS2 sample


title: 15_3g_UtrNK_A229_D1_S229 : SS2 sample


title: 11_10E_UcNK_A313_C1_S313 : SS2 sample


title: 15_3g_UtrNK_A215_B11_S215 : SS2 sample


title: 11_10E_UcNK_A314_C2_S314 : SS2 sample


title: 15_3g_UtrNK_A274_G10_S274 : SS2 sample


title: 15_3g_UtrNK_A258_F6_S258 : SS2 sample


title: 11_10E_UtrNK_A114_B6_S114 : SS2 sample


title: 15_3g_UtrNK_A269_G5_S269 : SS2 sample


title: 15_3g_UcNK_A10_A10_S10 : SS2 sample


title: 15_3g_UcNK_A4_A4_S4 : SS2 sample


title: 15_3g_UcNK_A86_H2_S86 : SS2 sample


title: 11_10E_UcNK_A317_C5_S317 : SS2 sample


title: 15_3g_UcNK_A11_A11_S11 : SS2 sample


title: 15_3g_UtrNK_A218_C2_S218 : SS2 sample


title: 11_10E_UtrNK_A160_F4_S160 : SS2 sample


title: 11_10E_UtrNK_A104_A8_S104 : SS2 sample


title: 15_3g_UcNK_A88_H4_S88 : SS2 sample


title: 15_3g_UtrNK_A248_E8_S248 : SS2 sample


title: 15_3g_UcNK_A66_F6_S66 : SS2 sample


title: 15_3g_UtrNK_A223_C7_S223 : SS2 sample


title: 15_3g_UcNK_A48_D12_S48 : SS2 sample


title: 11_10E_UtrNK_A105_A9_S105 : SS2 sample


title: 11_10E_UcNK_A319_C7_S319 : SS2 sample


title: 11_10E_UtrNK_A164_F8_S164 : SS2 sample


title: 15_3g_UtrNK_A276_G12_S276 : SS2 sample


title: 11_10E_UtrNK_A191_H11_S191 : SS2 sample


title: 15_3g_UcNK_A79_G7_S79 : SS2 sample


title: 15_3g_UcNK_A94_H10_S94 : SS2 sample


title: 15_3g_UcNK_A64_F4_S64 : SS2 sample


title: 11_10E_UtrNK_A103_A7_S103 : SS2 sample


title: 11_10E_UtrNK_A139_D7_S139 : SS2 sample


title: 15_3g_UcNK_A35_C11_S35 : SS2 sample


title: 15_3g_UtrNK_A210_B6_S210 : SS2 sample


title: 11_10E_UcNK_A359_F11_S359 : SS2 sample


title: 11_10E_UtrNK_A172_G4_S172 : SS2 sample


title: 11_10E_UcNK_A339_E3_S339 : SS2 sample


title: 15_3g_UcNK_A92_H8_S92 : SS2 sample


title: 15_3g_UtrNK_A275_G11_S275 : SS2 sample


title: 9_5B_UtrNK_A258_F6_S258 : SS2 sample


title: 15_3g_UcNK_A8_A8_S8 : SS2 sample


title: 15_3g_UtrNK_A246_E6_S246 : SS2 sample


title: 15_3g_UcNK_A24_B12_S24 : SS2 sample


title: 15_3g_UtrNK_A239_D11_S239 : SS2 sample


title: 11_10E_UtrNK_A138_D6_S138 : SS2 sample


title: 4_7H_UtrNK_A48_D12_S48 : SS2 sample


title: 9_5B_UcNK_A81_G9_S81 : SS2 sample


title: 8_1D_UtrNK_A2_A2_S2 : SS2 sample


title: 9_3G_UcNK_A323_C11_S323 : SS2 sample


title: 11_3E_UtrNK_A204_A12_S204 : SS2 sample


title: 15_3g_UcNK_A62_F2_S62 : SS2 sample


title: 11_3E_UcNK_A150_E6_S150 : SS2 sample


title: 15_4G_UtrNK_A166_F10_S166 : SS2 sample


title: 9_5B_UtrNK_A264_F12_S264 : SS2 sample


title: 15_4G_UcNK_A355_F7_S355 : SS2 sample


title: 15_4G_UtrNK_A167_F11_S167 : SS2 sample


title: 8_1D_UtrNK_A52_E4_S52 : SS2 sample


title: 11_1i_UcNK_A50_E2_S50 : SS2 sample


title: 15_4G_UcNK_A308_B8_S308 : SS2 sample


title: 11_3E_UtrNK_A267_G3_S267 : SS2 sample


title: 9_3G_UcNK_A382_H10_S382 : SS2 sample


title: 11_10E_UcNK_A383_H11_S383 : SS2 sample


title: 15_4G_UcNK_A346_E10_S346 : SS2 sample


title: 15_4G_UtrNK_A173_G5_S173 : SS2 sample


title: 11_1i_UcNK_A49_E1_S49 : SS2 sample


title: 11_3E_UtrNK_A286_H10_S286 : SS2 sample


title: 9_5B_UtrNK_A235_D7_S235 : SS2 sample


title: 9_5B_UtrNK_A272_G8_S272 : SS2 sample


title: 9_5B_UcNK_A5_A5_S5 : SS2 sample


title: 4_7H_UcNK_A274_G10_S274 : SS2 sample


title: 4_7H_UtrNK_A23_B11_S23 : SS2 sample


title: 15_4G_UcNK_A366_G6_S366 : SS2 sample


title: 8_1D_UtrNK_A58_E10_S58 : SS2 sample


title: 8_1D_UtrNK_A46_D10_S46 : SS2 sample


title: 4_7H_UtrNK_A7_A7_S7 : SS2 sample


title: 4_7H_UtrNK_A34_C10_S34 : SS2 sample


title: 8_1D_UtrNK_A14_B2_S14 : SS2 sample


title: 9_3G_UcNK_A360_F12_S360 : SS2 sample


title: 4_7H_UtrNK_A88_H4_S88 : SS2 sample


title: 11_1i_UcNK_A39_D3_S39 : SS2 sample


title: 9_5B_UcNK_A10_A10_S10 : SS2 sample


title: 11_3E_UcNK_A190_H10_S190 : SS2 sample


title: 15_4G_UtrNK_A127_C7_S127 : SS2 sample


title: 11_1i_UcNK_A86_H2_S86 : SS2 sample


title: 11_1i_UcNK_A11_A11_S11 : SS2 sample


title: 11_3E_UcNK_A100_A4_S100 : SS2 sample


title: 15_4G_UcNK_A327_D3_S327 : SS2 sample


title: 9_5B_UtrNK_A245_E5_S245 : SS2 sample


title: 4_7H_UtrNK_A30_C6_S30 : SS2 sample


title: 9_5B_UtrNK_A234_D6_S234 : SS2 sample


title: 11_3E_UcNK_A143_D11_S143 : SS2 sample


title: 9_5B_UcNK_A4_A4_S4 : SS2 sample


title: 11_3E_UcNK_A135_D3_S135 : SS2 sample


title: 11_3E_UtrNK_A284_H8_S284 : SS2 sample


title: 11_1i_UtrNK_A304_B4_S304 : SS2 sample


title: 11_3E_UcNK_A180_G12_S180 : SS2 sample


title: 9_3G_UcNK_A309_B9_S309 : SS2 sample


title: 11_1i_UcNK_A45_D9_S45 : SS2 sample


title: 11_1i_UcNK_A69_F9_S69 : SS2 sample


title: 15_4G_UtrNK_A149_E5_S149 : SS2 sample


title: 9_3G_UcNK_A352_F4_S352 : SS2 sample


title: 8_1D_UtrNK_A85_H1_S85 : SS2 sample


title: 9_3G_UtrNK_A166_F10_S166 : SS2 sample


title: 4_7H_UtrNK_A87_H3_S87 : SS2 sample


title: 9_3G_UcNK_A335_D11_S335 : SS2 sample


title: 11_3E_UcNK_A137_D5_S137 : SS2 sample


title: 9_5B_UcNK_A80_G8_S80 : SS2 sample


title: 9_5B_UtrNK_A237_D9_S237 : SS2 sample


title: 9_5B_UtrNK_A231_D3_S231 : SS2 sample


title: 11_1i_UtrNK_A361_G1_S361 : SS2 sample


title: 9_5B_UtrNK_A214_B10_S214 : SS2 sample


title: 4_7H_UcNK_A200_A8_S200 : SS2 sample


title: 8_1D_UcNK_A212_B8_S212 : SS2 sample


title: 9_5B_UtrNK_A285_H9_S285 : SS2 sample


title: 9_5B_UtrNK_A273_G9_S273 : SS2 sample


title: 9_5B_UtrNK_A223_C7_S223 : SS2 sample


title: 4_7H_UcNK_A266_G2_S266 : SS2 sample


title: 9_3G_UcNK_A334_D10_S334 : SS2 sample


title: 11_1i_UcNK_A62_F2_S62 : SS2 sample


title: 4_7H_UcNK_A255_F3_S255 : SS2 sample


title: 9_5B_UtrNK_A268_G4_S268 : SS2 sample


title: 11_1i_UcNK_S1 : SS2 sample


title: 15_4G_UtrNK_A110_B2_S110 : SS2 sample


title: 9_3G_UcNK_A344_E8_S344 : SS2 sample


title: 9_3G_UcNK_A318_C6_S318 : SS2 sample


title: 11_3E_UcNK_A120_B12_S120 : SS2 sample


title: 11_1i_UtrNK_A297_A9_S297 : SS2 sample


title: 8_1D_UtrNK_A89_H5_S89 : SS2 sample


title: 11_1i_UcNK_A21_B9_S21 : SS2 sample


title: 8_1D_UtrNK_A11_A11_S11 : SS2 sample


title: 15_4G_UtrNK_A100_A4_S100 : SS2 sample


title: 15_4G_UtrNK_A101_A5_S101 : SS2 sample


title: 15_4G_UcNK_A311_B11_S311 : SS2 sample


title: 15_4G_UtrNK_A139_D7_S139 : SS2 sample


title: 9_3G_UtrNK_A114_B6_S114 : SS2 sample


title: 11_3E_UtrNK_A194_A2_S194 : SS2 sample


title: 8_1D_UcNK_A254_F2_S254 : SS2 sample


title: 11_1i_UtrNK_A353_F5_S353 : SS2 sample


title: 11_1i_UtrNK_A291_A3_S291 : SS2 sample


title: 9_5B_UtrNK_A287_H11_S287 : SS2 sample


title: 15_4G_UtrNK_A165_F9_S165 : SS2 sample


title: 11_1i_UcNK_A52_E4_S52 : SS2 sample


title: 8_1D_UcNK_A267_G3_S267 : SS2 sample


title: 9_3G_UcNK_A333_D9_S333 : SS2 sample


title: 8_1D_UcNK_A281_H5_S281 : SS2 sample


title: 8_1D_UcNK_A216_B12_S216 : SS2 sample


title: 9_5B_UtrNK_A226_C10_S226 : SS2 sample


title: 9_3G_UtrNK_A110_B2_S110 : SS2 sample


title: 11_1i_UcNK_A26_C2_S26 : SS2 sample


title: 9_3G_UtrNK_A111_B3_S111 : SS2 sample


title: 11_3E_UcNK_A142_D10_S142 : SS2 sample


title: 4_7H_UtrNK_A33_C9_S33 : SS2 sample


title: 11_1i_UcNK_A76_G4_S76 : SS2 sample


title: 8_1D_UcNK_A220_C4_S220 : SS2 sample


title: 11_3E_UtrNK_A247_E7_S247 : SS2 sample


title: 15_4G_UtrNK_A172_G4_S172 : SS2 sample


title: 8_1D_UtrNK_A29_C5_S29 : SS2 sample


title: 15_4G_UcNK_A349_F1_S349 : SS2 sample


title: 4_7H_UcNK_A265_G1_S265 : SS2 sample


title: 4_7H_UcNK_A228_C12_S228 : SS2 sample


title: 11_1i_UtrNK_A311_B11_S311 : SS2 sample


title: 15_4G_UtrNK_A174_G6_S174 : SS2 sample


title: 9_5B_UtrNK_A216_B12_S216 : SS2 sample


title: 4_7H_UtrNK_A74_G2_S74 : SS2 sample


title: 15_4G_UtrNK_A151_E7_S151 : SS2 sample


title: 8_1D_UtrNK_A93_H9_S93 : SS2 sample


title: 11_3E_UcNK_A185_H5_S185 : SS2 sample


title: 11_1i_UtrNK_A324_C12_S324 : SS2 sample


title: 4_7H_UtrNK_A61_F1_S61 : SS2 sample


title: 8_1D_UcNK_A261_F9_S261 : SS2 sample


title: 8_1D_UtrNK_A45_D9_S45 : SS2 sample


title: 8_1D_UcNK_A282_H6_S282 : SS2 sample


title: 4_7H_UcNK_A268_G4_S268 : SS2 sample


title: 9_3G_UcNK_A297_A9_S297 : SS2 sample


title: 9_3G_UcNK_A324_C12_S324 : SS2 sample


title: 8_1D_UcNK_A268_G4_S268 : SS2 sample


title: 11_3E_UcNK_A108_A12_S108 : SS2 sample


title: 11_1i_UcNK_A25_C1_S25 : SS2 sample


title: 15_4G_UcNK_A376_H4_S376 : SS2 sample


title: 15_4G_UtrNK_A120_B12_S120 : SS2 sample


title: 11_1i_UcNK_A82_G10_S82 : SS2 sample


title: 11_3E_UcNK_A104_A8_S104 : SS2 sample


title: 9_5B_UcNK_A61_F1_S61 : SS2 sample


title: 11_3E_UtrNK_A223_C7_S223 : SS2 sample


title: 9_5B_UtrNK_A221_C5_S221 : SS2 sample


title: 11_1i_UcNK_A92_H8_S92 : SS2 sample


title: 9_5B_UtrNK_A193_A1_S193 : SS2 sample


title: 9_3G_UtrNK_A133_D1_S133 : SS2 sample


title: 9_5B_UtrNK_A275_G11_S275 : SS2 sample


title: 4_7H_UcNK_A233_D5_S233 : SS2 sample


title: 9_5B_UcNK_A71_F11_S71 : SS2 sample


title: 15_4G_UcNK_A384_H12_S384 : SS2 sample


title: 9_3G_UtrNK_A128_C8_S128 : SS2 sample


title: 11_1i_UcNK_A16_B4_S16 : SS2 sample


title: 9_3G_UtrNK_A153_E9_S153 : SS2 sample


title: 11_3E_UcNK_A158_F2_S158 : SS2 sample


title: 4_7H_UcNK_A225_C9_S225 : SS2 sample


title: 4_7H_UcNK_A231_D3_S231 : SS2 sample


title: 11_1i_UtrNK_A380_H8_S380 : SS2 sample


title: 9_3G_UcNK_A366_G6_S366 : SS2 sample


title: 11_3E_UtrNK_A218_C2_S218 : SS2 sample


title: 11_1i_UtrNK_A341_E5_S341 : SS2 sample


title: 8_1D_UtrNK_A72_F12_S72 : SS2 sample


title: 9_5B_UtrNK_A225_C9_S225 : SS2 sample


title: 9_5B_UcNK_A49_E1_S49 : SS2 sample


title: 8_1D_UcNK_A206_B2_S206 : SS2 sample


title: 9_3G_UtrNK_A191_H11_S191 : SS2 sample


title: 11_1i_UtrNK_A292_A4_S292 : SS2 sample


title: 9_3G_UcNK_A376_H4_S376 : SS2 sample


title: 11_1i_UcNK_A74_G2_S74 : SS2 sample


title: 4_7H_UtrNK_A70_F10_S70 : SS2 sample


title: 8_1D_UtrNK_A92_H8_S92 : SS2 sample


title: 15_4G_UtrNK_A147_E3_S147 : SS2 sample


title: 11_3E_UtrNK_A229_D1_S229 : SS2 sample


title: 8_1D_UcNK_A244_E4_S244 : SS2 sample


title: 9_5B_UcNK_A94_H10_S94 : SS2 sample


title: 11_3E_UtrNK_A273_G9_S273 : SS2 sample


title: 11_1i_UtrNK_A343_E7_S343 : SS2 sample


title: 11_1i_UtrNK_A325_D1_S325 : SS2 sample


title: 11_1i_UtrNK_A345_E9_S345 : SS2 sample


title: 11_1i_UcNK_A70_F10_S70 : SS2 sample


title: 9_3G_UcNK_A312_B12_S312 : SS2 sample


title: 4_7H_UcNK_A232_D4_S232 : SS2 sample


title: 11_1i_UcNK_A63_F3_S63 : SS2 sample


title: 11_3E_UcNK_A105_A9_S105 : SS2 sample


title: 4_7H_UcNK_A230_D2_S230 : SS2 sample


title: 11_3E_UtrNK_A251_E11_S251 : SS2 sample


title: 9_5B_UtrNK_A229_D1_S229 : SS2 sample


title: 11_3E_UcNK_A169_G1_S169 : SS2 sample


title: 15_4G_UcNK_A343_E7_S343 : SS2 sample


title: 9_3G_UcNK_A310_B10_S310 : SS2 sample


title: 9_3G_UtrNK_A162_F6_S162 : SS2 sample


title: 9_5B_UtrNK_A284_H8_S284 : SS2 sample


title: 9_3G_UcNK_A298_A10_S298 : SS2 sample


title: 11_3E_UtrNK_A225_C9_S225 : SS2 sample


title: 11_3E_UtrNK_A215_B11_S215 : SS2 sample


title: 9_3G_UtrNK_A124_C4_S124 : SS2 sample


title: 8_1D_UcNK_A210_B6_S210 : SS2 sample


title: 8_1D_UcNK_A280_H4_S280 : SS2 sample


title: 4_7H_UtrNK_A65_F5_S65 : SS2 sample


title: 4_7H_UtrNK_A86_H2_S86 : SS2 sample


title: 4_7H_UtrNK_A72_F12_S72 : SS2 sample


title: 11_1i_UcNK_A84_G12_S84 : SS2 sample


title: 4_7H_UcNK_A193_A1_S193 : SS2 sample


title: 11_1i_UtrNK_A365_G5_S365 : SS2 sample


title: 8_1D_UtrNK_A88_H4_S88 : SS2 sample


title: 11_1i_UcNK_A71_F11_S71 : SS2 sample


title: 15_4G_UcNK_A312_B12_S312 : SS2 sample


title: 9_3G_UtrNK_A152_E8_S152 : SS2 sample


title: 11_1i_UtrNK_A356_F8_S356 : SS2 sample


title: 15_4G_UtrNK_A108_A12_S108 : SS2 sample


title: 15_4G_UtrNK_A125_C5_S125 : SS2 sample


title: 8_1D_UcNK_A270_G6_S270 : SS2 sample


title: 4_7H_UtrNK_A45_D9_S45 : SS2 sample


title: 11_1i_UcNK_A5_A5_S5 : SS2 sample


title: 4_7H_UtrNK_A4_A4_S4 : SS2 sample


title: 11_3E_UtrNK_A198_A6_S198 : SS2 sample


title: 9_3G_UcNK_A368_G8_S368 : SS2 sample


title: 11_3E_UtrNK_A201_A9_S201 : SS2 sample


title: 15_4G_UcNK_A348_E12_S348 : SS2 sample


title: 9_3G_UcNK_A326_D2_S326 : SS2 sample


title: 9_5B_UtrNK_A256_F4_S256 : SS2 sample


title: 11_1i_UcNK_A68_F8_S68 : SS2 sample


title: 9_5B_UtrNK_A196_A4_S196 : SS2 sample


title: 8_1D_UtrNK_A47_D11_S47 : SS2 sample


title: 4_7H_UtrNK_A15_B3_S15 : SS2 sample


title: 4_7H_UtrNK_A21_B9_S21 : SS2 sample


title: 15_4G_UtrNK_A132_C12_S132 : SS2 sample


title: 15_4G_UtrNK_A177_G9_S177 : SS2 sample


title: 15_4G_UtrNK_A114_B6_S114 : SS2 sample


title: 9_3G_UcNK_A296_A8_S296 : SS2 sample


title: 9_5B_UtrNK_A236_D8_S236 : SS2 sample


title: 15_4G_UtrNK_A105_A9_S105 : SS2 sample


title: 8_1D_UtrNK_A96_H12_S96 : SS2 sample


title: 9_5B_UcNK_A51_E3_S51 : SS2 sample


title: 8_1D_UtrNK_A7_A7_S7 : SS2 sample


title: 9_3G_UcNK_A341_E5_S341 : SS2 sample


title: 15_4G_UtrNK_A178_G10_S178 : SS2 sample


title: 15_4G_UtrNK_A111_B3_S111 : SS2 sample


title: 11_3E_UtrNK_A234_D6_S234 : SS2 sample


title: 11_1i_UcNK_A28_C4_S28 : SS2 sample


title: 11_3E_UtrNK_A280_H4_S280 : SS2 sample


title: 11_3E_UtrNK_A193_A1_S193 : SS2 sample


title: 9_3G_UcNK_A330_D6_S330 : SS2 sample


title: 9_5B_UcNK_A12_A12_S12 : SS2 sample


title: 11_3E_UcNK_A145_E1_S145 : SS2 sample


title: 8_1D_UtrNK_A74_G2_S74 : SS2 sample


title: 4_7H_UtrNK_A51_E3_S51 : SS2 sample


title: 9_5B_UtrNK_A274_G10_S274 : SS2 sample


title: 15_4G_UtrNK_A179_G11_S179 : SS2 sample


title: 11_3E_UcNK_A98_A2_S98 : SS2 sample


title: 8_1D_UcNK_A235_D7_S235 : SS2 sample


title: 11_1i_UtrNK_A313_C1_S313 : SS2 sample


title: 11_3E_UtrNK_A263_F11_S263 : SS2 sample


title: 11_3E_UcNK_A166_F10_S166 : SS2 sample


title: 11_1i_UtrNK_A327_D3_S327 : SS2 sample


title: 9_3G_UcNK_A337_E1_S337 : SS2 sample


title: 8_1D_UtrNK_A71_F11_S71 : SS2 sample


title: 15_4G_UcNK_A347_E11_S347 : SS2 sample


title: 11_3E_UcNK_A97_A1_S97 : SS2 sample


title: 11_1i_UtrNK_A339_E3_S339 : SS2 sample


title: 4_7H_UcNK_A248_E8_S248 : SS2 sample


title: 4_7H_UtrNK_A69_F9_S69 : SS2 sample


title: 9_3G_UtrNK_A170_G2_S170 : SS2 sample


title: 8_1D_UcNK_A217_C1_S217 : SS2 sample


title: 9_5B_UtrNK_A255_F3_S255 : SS2 sample


title: 9_3G_UcNK_A377_H5_S377 : SS2 sample


title: 9_5B_UcNK_A73_G1_S73 : SS2 sample


title: 4_7H_UtrNK_A14_B2_S14 : SS2 sample


title: 9_5B_UtrNK_A215_B11_S215 : SS2 sample


title: 11_3E_UcNK_A136_D4_S136 : SS2 sample


title: 8_1D_UcNK_A229_D1_S229 : SS2 sample


title: 11_3E_UtrNK_A243_E3_S243 : SS2 sample


title: 8_1D_UtrNK_A78_G6_S78 : SS2 sample


title: 9_3G_UcNK_A325_D1_S325 : SS2 sample


title: 8_1D_UcNK_A245_E5_S245 : SS2 sample


title: 11_3E_UcNK_A178_G10_S178 : SS2 sample


title: 9_3G_UtrNK_A143_D11_S143 : SS2 sample


title: 4_7H_UcNK_A254_F2_S254 : SS2 sample


title: 11_1i_UtrNK_A318_C6_S318 : SS2 sample


title: 4_7H_UtrNK_A62_F2_S62 : SS2 sample


title: 9_3G_UtrNK_A148_E4_S148 : SS2 sample


title: 11_1i_UcNK_A23_B11_S23 : SS2 sample


title: 9_3G_UtrNK_A157_F1_S157 : SS2 sample


title: 11_1i_UtrNK_A312_B12_S312 : SS2 sample


title: 11_1i_UcNK_A12_A12_S12 : SS2 sample


title: 9_5B_UcNK_A63_F3_S63 : SS2 sample


title: 4_7H_UtrNK_A8_A8_S8 : SS2 sample


title: 9_3G_UtrNK_A177_G9_S177 : SS2 sample


title: 11_3E_UtrNK_A231_D3_S231 : SS2 sample


title: 8_1D_UtrNK_A1_A1_S1 : SS2 sample


title: 11_3E_UtrNK_A246_E6_S246 : SS2 sample


title: 8_1D_UtrNK_A16_B4_S16 : SS2 sample


title: 4_7H_UcNK_A197_A5_S197 : SS2 sample


title: 9_5B_UtrNK_A280_H4_S280 : SS2 sample


title: 8_1D_UtrNK_A12_A12_S12 : SS2 sample


title: 9_5B_UcNK_A43_D7_S43 : SS2 sample


title: 15_4G_UtrNK_A136_D4_S136 : SS2 sample


title: 9_3G_UtrNK_A106_A10_S106 : SS2 sample


title: 4_7H_UcNK_A235_D7_S235 : SS2 sample


title: 9_5B_UcNK_A33_C9_S33 : SS2 sample


title: 15_4G_UcNK_A321_C9_S321 : SS2 sample


title: 11_3E_UcNK_A176_G8_S176 : SS2 sample


title: 11_3E_UtrNK_A236_D8_S236 : SS2 sample


title: 4_7H_UtrNK_A84_G12_S84 : SS2 sample


title: 9_5B_UcNK_A37_D1_S37 : SS2 sample


title: 9_3G_UcNK_A307_B7_S307 : SS2 sample


title: 4_7H_UcNK_A245_E5_S245 : SS2 sample


title: 8_1D_UtrNK_A84_G12_S84 : SS2 sample


title: 9_5B_UcNK_A79_G7_S79 : SS2 sample


title: 15_4G_UcNK_A331_D7_S331 : SS2 sample


title: 15_4G_UtrNK_A169_G1_S169 : SS2 sample


title: 9_3G_UtrNK_A176_G8_S176 : SS2 sample


title: 8_1D_UtrNK_A41_D5_S41 : SS2 sample


title: 9_3G_UtrNK_A99_A3_S99 : SS2 sample


title: 9_5B_UcNK_A48_D12_S48 : SS2 sample


title: 4_7H_UcNK_A260_F8_S260 : SS2 sample


title: 9_3G_UcNK_A373_H1_S373 : SS2 sample


title: 11_3E_UcNK_A183_H3_S183 : SS2 sample


title: 4_7H_UtrNK_A56_E8_S56 : SS2 sample


title: 9_5B_UtrNK_A198_A6_S198 : SS2 sample


title: 4_7H_UtrNK_A58_E10_S58 : SS2 sample


title: 11_3E_UtrNK_A249_E9_S249 : SS2 sample


title: 4_7H_UtrNK_A64_F4_S64 : SS2 sample


title: 15_4G_UtrNK_A144_D12_S144 : SS2 sample


title: 9_5B_UtrNK_A209_B5_S209 : SS2 sample


title: 11_1i_UcNK_A67_F7_S67 : SS2 sample


title: 15_4G_UcNK_A356_F8_S356 : SS2 sample


title: 15_4G_UtrNK_A124_C4_S124 : SS2 sample


title: 8_1D_UcNK_A226_C10_S226 : SS2 sample


title: 8_1D_UcNK_A284_H8_S284 : SS2 sample


title: 8_1D_UtrNK_A26_C2_S26 : SS2 sample


title: 15_4G_UtrNK_A150_E6_S150 : SS2 sample


title: 4_7H_UtrNK_A53_E5_S53 : SS2 sample


title: 15_4G_UcNK_A318_C6_S318 : SS2 sample


title: 11_1i_UcNK_A47_D11_S47 : SS2 sample


title: 9_5B_UtrNK_A200_A8_S200 : SS2 sample


title: 9_5B_UtrNK_A205_B1_S205 : SS2 sample


title: 4_7H_UtrNK_A26_C2_S26 : SS2 sample


title: 8_1D_UcNK_A203_A11_S203 : SS2 sample


title: 8_1D_UtrNK_A22_B10_S22 : SS2 sample


title: 11_3E_UcNK_A161_F5_S161 : SS2 sample


title: 4_7H_UtrNK_A46_D10_S46 : SS2 sample


title: 9_5B_UcNK_A69_F9_S69 : SS2 sample


title: 9_3G_UtrNK_A169_G1_S169 : SS2 sample


title: 4_7H_UtrNK_A60_E12_S60 : SS2 sample


title: 11_1i_UtrNK_A381_H9_S381 : SS2 sample


title: 9_5B_UtrNK_A288_H12_S288 : SS2 sample


title: 8_1D_UtrNK_A56_E8_S56 : SS2 sample


title: 11_3E_UcNK_A151_E7_S151 : SS2 sample


title: 9_5B_UtrNK_A199_A7_S199 : SS2 sample


title: 9_3G_UtrNK_A161_F5_S161 : SS2 sample


title: 4_7H_UcNK_A198_A6_S198 : SS2 sample


title: 9_3G_UcNK_A336_D12_S336 : SS2 sample


title: 9_5B_UcNK_A67_F7_S67 : SS2 sample


title: 15_4G_UtrNK_A109_B1_S109 : SS2 sample


title: 4_7H_UtrNK_A20_B8_S20 : SS2 sample


title: 9_5B_UtrNK_A242_E2_S242 : SS2 sample


title: 4_7H_UtrNK_A25_C1_S25 : SS2 sample


title: 4_7H_UcNK_A211_B7_S211 : SS2 sample


title: 9_3G_UcNK_A327_D3_S327 : SS2 sample


title: 9_3G_UcNK_A294_A6_S294 : SS2 sample


title: 11_3E_UcNK_A127_C7_S127 : SS2 sample


title: 4_7H_UcNK_A246_E6_S246 : SS2 sample


title: 9_3G_UtrNK_A142_D10_S142 : SS2 sample


title: 4_7H_UcNK_A203_A11_S203 : SS2 sample


title: 11_1i_UcNK_A44_D8_S44 : SS2 sample


title: 11_3E_UcNK_A106_A10_S106 : SS2 sample


title: 4_7H_UtrNK_A6_A6_S6 : SS2 sample


title: 9_3G_UcNK_A331_D7_S331 : SS2 sample


title: 11_3E_UcNK_A174_G6_S174 : SS2 sample


title: 4_7H_UtrNK_A85_H1_S85 : SS2 sample


title: 4_7H_UtrNK_A22_B10_S22 : SS2 sample


title: 9_5B_UtrNK_A210_B6_S210 : SS2 sample


title: 8_1D_UcNK_A238_D10_S238 : SS2 sample


title: 11_3E_UcNK_A184_H4_S184 : SS2 sample


title: 11_1i_UcNK_A53_E5_S53 : SS2 sample


title: 15_4G_UcNK_A314_C2_S314 : SS2 sample


title: 8_1D_UtrNK_A70_F10_S70 : SS2 sample


title: 9_5B_UtrNK_A251_E11_S251 : SS2 sample


title: 4_7H_UcNK_A250_E10_S250 : SS2 sample


title: 11_3E_UcNK_A146_E2_S146 : SS2 sample


title: 15_4G_UcNK_A299_A11_S299 : SS2 sample


title: 9_3G_UtrNK_A173_G5_S173 : SS2 sample


title: 11_3E_UcNK_A99_A3_S99 : SS2 sample


title: 11_3E_UtrNK_A217_C1_S217 : SS2 sample


title: 9_3G_UtrNK_A185_H5_S185 : SS2 sample


title: 9_5B_UcNK_A68_F8_S68 : SS2 sample


title: 15_4G_UcNK_A374_H2_S374 : SS2 sample


title: 11_3E_UtrNK_A197_A5_S197 : SS2 sample


title: 9_3G_UtrNK_A113_B5_S113 : SS2 sample


title: 9_3G_UtrNK_A125_C5_S125 : SS2 sample


title: 9_3G_UcNK_A313_C1_S313 : SS2 sample


title: 4_7H_UtrNK_A47_D11_S47 : SS2 sample


title: 11_3E_UtrNK_A266_G2_S266 : SS2 sample


title: 11_1i_UtrNK_A354_F6_S354 : SS2 sample


title: 15_4G_UcNK_A350_F2_S350 : SS2 sample


title: 4_7H_UtrNK_A13_B1_S13 : SS2 sample


title: 8_1D_UtrNK_A95_H11_S95 : SS2 sample


title: 8_1D_UcNK_A223_C7_S223 : SS2 sample


title: 11_1i_UcNK_A61_F1_S61 : SS2 sample


title: 8_1D_UtrNK_A18_B6_S18 : SS2 sample


title: 9_5B_UcNK_A27_C3_S27 : SS2 sample


title: 15_4G_UcNK_A381_H9_S381 : SS2 sample


title: 8_1D_UcNK_A218_C2_S218 : SS2 sample


title: 11_1i_UcNK_A41_D5_S41 : SS2 sample


title: 9_5B_UcNK_A83_G11_S83 : SS2 sample


title: 15_4G_UcNK_A373_H1_S373 : SS2 sample


title: 15_4G_UtrNK_A160_F4_S160 : SS2 sample


title: 15_4G_UtrNK_A117_B9_S117 : SS2 sample


title: 15_4G_UcNK_A324_C12_S324 : SS2 sample


title: 15_4G_UtrNK_A183_H3_S183 : SS2 sample


title: 9_5B_UtrNK_A262_F10_S262 : SS2 sample


title: 8_1D_UcNK_A228_C12_S228 : SS2 sample


title: 4_7H_UcNK_A285_H9_S285 : SS2 sample


title: 11_3E_UtrNK_A241_E1_S241 : SS2 sample


title: 4_7H_UtrNK_A1_A1_S1 : SS2 sample


title: 15_4G_UcNK_A335_D11_S335 : SS2 sample


title: 11_1i_UtrNK_A375_H3_S375 : SS2 sample


title: 11_3E_UtrNK_A252_E12_S252 : SS2 sample


title: 9_5B_UcNK_A55_E7_S55 : SS2 sample


title: 11_1i_UtrNK_A300_A12_S300 : SS2 sample


title: 15_4G_UtrNK_A112_B4_S112 : SS2 sample


title: 15_4G_UcNK_A340_E4_S340 : SS2 sample


title: 11_3E_UtrNK_A238_D10_S238 : SS2 sample


title: 11_3E_UtrNK_A261_F9_S261 : SS2 sample


title: 11_1i_UtrNK_A322_C10_S322 : SS2 sample


title: 8_1D_UcNK_A246_E6_S246 : SS2 sample


title: 11_3E_UtrNK_A275_G11_S275 : SS2 sample


title: 8_1D_UcNK_A274_G10_S274 : SS2 sample


title: 9_3G_UtrNK_A105_A9_S105 : SS2 sample


title: 11_1i_UtrNK_A347_E11_S347 : SS2 sample


title: 11_1i_UtrNK_A329_D5_S329 : SS2 sample


title: 15_4G_UcNK_A301_B1_S301 : SS2 sample


title: 9_3G_UcNK_A351_F3_S351 : SS2 sample


title: 11_1i_UcNK_A15_B3_S15 : SS2 sample


title: 11_3E_UtrNK_A265_G1_S265 : SS2 sample


title: 11_1i_UtrNK_A371_G11_S371 : SS2 sample


title: 9_3G_UtrNK_A120_B12_S120 : SS2 sample


title: 8_1D_UtrNK_A59_E11_S59 : SS2 sample


title: 15_4G_UcNK_A296_A8_S296 : SS2 sample


title: 15_4G_UcNK_A341_E5_S341 : SS2 sample


title: 9_5B_UtrNK_A238_D10_S238 : SS2 sample


title: 9_3G_UtrNK_A123_C3_S123 : SS2 sample


title: 11_1i_UcNK_A37_D1_S37 : SS2 sample


title: 11_1i_UtrNK_A340_E4_S340 : SS2 sample


title: 11_1i_UtrNK_A367_G7_S367 : SS2 sample


title: 11_3E_UcNK_A189_H9_S189 : SS2 sample


title: 32_3c_UcNK_A87_H3_S87 : SS2 sample


title: 37_2D_UcNK_A340_E4_S340 : SS2 sample


title: 32_4c_UtrNK_A217_C1_S217 : SS2 sample


title: 32_3c_UcNK_A94_H10_S94 : SS2 sample


title: 33_1G_UtrNK_A130_C10_S130 : SS2 sample


title: 8_1D_UcNK_A265_G1_S265 : SS2 sample


title: 32_3c_UcNK_A39_D3_S39 : SS2 sample


title: 32_3c_UtrNK_A256_F4_S256 : SS2 sample


title: 37_2D_UcNK_A375_H3_S375 : SS2 sample


title: 33_1G_UcNK_A298_A10_S298 : SS2 sample


title: 15_3F_UtrNK_A232_D4_S232 : SS2 sample


title: 33_1G_UcNK_A290_A2_S290 : SS2 sample


title: 7_3J_UtrNK_A126_C6_S126 : SS2 sample


title: 33_1G_UtrNK_A175_G7_S175 : SS2 sample


title: 32_4c_UtrNK_A229_D1_S229 : SS2 sample


title: 15_3F_UtrNK_A264_F12_S264 : SS2 sample


title: 33_1G_UcNK_A296_A8_S296 : SS2 sample


title: 33_1G_UtrNK_A192_H12_S192 : SS2 sample


title: 33_1G_UcNK_A338_E2_S338 : SS2 sample


title: 32_3c_UtrNK_A239_D11_S239 : SS2 sample


title: 37_2D_UtrNK_A113_B5_S113 : SS2 sample


title: 37_2D_UtrNK_A174_G6_S174 : SS2 sample


title: 32_3c_UcNK_A36_C12_S36 : SS2 sample


title: 33_1G_UtrNK_A168_F12_S168 : SS2 sample


title: 7_3J_UcNK_A301_B1_S301 : SS2 sample


title: 32_3c_UcNK_A27_C3_S27 : SS2 sample


title: 32_4c_UcNK_A11_A11_S11 : SS2 sample


title: 33_1G_UcNK_A376_H4_S376 : SS2 sample


title: 32_4c_UcNK_A62_F2_S62 : SS2 sample


title: 33_1G_UcNK_A309_B9_S309 : SS2 sample


title: 37_2D_UcNK_A339_E3_S339 : SS2 sample


title: 33_1G_UcNK_A369_G9_S369 : SS2 sample


title: 37_2D_UtrNK_A166_F10_S166 : SS2 sample


title: 7_3J_UtrNK_A181_H1_S181 : SS2 sample


title: 33_1G_UtrNK_A156_E12_S156 : SS2 sample


title: 7_3J_UtrNK_A142_D10_S142 : SS2 sample


title: 7_3J_UcNK_A376_H4_S376 : SS2 sample


title: 15_3F_UcNK_A17_B5_S17 : SS2 sample


title: 32_3c_UcNK_A63_F3_S63 : SS2 sample


title: 33_1G_UtrNK_A144_D12_S144 : SS2 sample


title: 32_4c_UcNK_A70_F10_S70 : SS2 sample


title: 37_2D_UtrNK_A157_F1_S157 : SS2 sample


title: 15_3F_UtrNK_A239_D11_S239 : SS2 sample


title: 32_4c_UtrNK_A204_A12_S204 : SS2 sample


title: 37_2D_UcNK_A319_C7_S319 : SS2 sample


title: 32_3c_UcNK_A18_B6_S18 : SS2 sample


title: 32_4c_UtrNK_A256_F4_S256 : SS2 sample


title: 32_4c_UtrNK_A270_G6_S270 : SS2 sample


title: 33_1G_UtrNK_A167_F11_S167 : SS2 sample


title: 7_3J_UcNK_A322_C10_S322 : SS2 sample


title: 32_3c_UtrNK_A230_D2_S230 : SS2 sample


title: 32_3c_UtrNK_A253_F1_S253 : SS2 sample


title: 15_3F_UcNK_A12_A12_S12 : SS2 sample


title: 32_3c_UtrNK_A222_C6_S222 : SS2 sample


title: 33_1G_UcNK_A302_B2_S302 : SS2 sample


title: 32_3c_UtrNK_A244_E4_S244 : SS2 sample


title: 32_3c_UtrNK_A279_H3_S279 : SS2 sample


title: 37_2D_UcNK_A292_A4_S292 : SS2 sample


title: 7_3J_UcNK_A362_G2_S362 : SS2 sample


title: 15_3F_UcNK_A88_H4_S88 : SS2 sample


title: 32_4c_UtrNK_A272_G8_S272 : SS2 sample


title: 33_1G_UcNK_A343_E7_S343 : SS2 sample


title: 32_3c_UcNK_A56_E8_S56 : SS2 sample


title: 33_1G_UcNK_A335_D11_S335 : SS2 sample


title: 33_1G_UtrNK_A147_E3_S147 : SS2 sample


title: 37_2D_UcNK_A336_D12_S336 : SS2 sample


title: 37_2D_UtrNK_A100_A4_S100 : SS2 sample


title: 37_2D_UtrNK_A177_G9_S177 : SS2 sample


title: 37_2D_UcNK_A347_E11_S347 : SS2 sample


title: 32_3c_UtrNK_A207_B3_S207 : SS2 sample


title: 33_1G_UtrNK_A171_G3_S171 : SS2 sample


title: 15_3F_UtrNK_A269_G5_S269 : SS2 sample


title: 32_4c_UcNK_A54_E6_S54 : SS2 sample


title: 33_1G_UtrNK_A131_C11_S131 : SS2 sample


title: 15_3F_UtrNK_A270_G6_S270 : SS2 sample


title: 32_3c_UcNK_A17_B5_S17 : SS2 sample


title: 15_3F_UcNK_A62_F2_S62 : SS2 sample


title: 7_3J_UtrNK_A120_B12_S120 : SS2 sample


title: 15_3F_UtrNK_A203_A11_S203 : SS2 sample


title: 15_3F_UcNK_A6_A6_S6 : SS2 sample


title: 15_3F_UtrNK_A208_B4_S208 : SS2 sample


title: 37_2D_UtrNK_A123_C3_S123 : SS2 sample


title: 15_3F_UcNK_A23_B11_S23 : SS2 sample