title: HIF1a ARNT round 3

value: human


title: Random 18mer library

status: Public on Jul 02 2022

type: SRA

city: Adelaide

first: David

middle: C

last: Bersten

state: SA

address: Victoria Drive

department: Molecular and Biomedical Sciences

country: Australia

institute: The University of Adelaide

description: Library 18mer

library_source: genomic

data_processing:
Library strategy: SELEX-seq
base-calling and demultiplexing was achieved with bcl2fastq,
fastq files were analysed for quality using Fastx toolkit (Hannon Lab, version 0.0.13). In some analysis, Kmer enrichment and transcription factor binding models were generated with filtered fastq data to remove more than one “Core” binding site per read.
One Core motif per read was filtered using fastq-tools (version 0.8) fast-grep -v function. Kmer counting and relative enrichments were analysed using the SELEX-seq R package (version 1.2), transcription factor binding motif models generated using No Read Left Behind (NRLB) https://github.com/BussemakerLab/NRLB. Demultiplexing and trimming of primers and barcode sequences were removed using SELEX-seq or NRLB during analysis by specifying flanks and barcodes.
Supplementary_files_format_and_content: read count of kmers

molecule: genomic DNA

organism: synthetic construct

source_name: Synthetic

growth_protocol: We purified full length human ARNT (1-774) or ARNT2 (1-717) homodimers from Expi293 cells, bHLH-PAS (PAS A and PAS B, N-terminus) heterdimers (human HIF1a(1-375)/ARNT(1-474), human HIF2a(1-353)/ARNT(1-474), human SIM1(1-348)/ARNT2(1-439) and mouse NPAS4(1-329)/ARNT2(1-481)) from baculovirus infected Sf9 insect cells or human AhR(1-287)/ARNT(1-364) from bacteria.    Baculoviral expression and purification  MultiBAC-LoxP-EYFP was generated by recombination of pUDCM-EYFP into the MultiBAC genome by Cre transposition as described Berger I et al, Nature Biotechnology 2004. With the exception of NPAS4 all truncated bHLH-PAS (A and B) heterodimers were incorporated into the MultiBAC-LoxP-EYFP baculoviral genome by pFBDM Tn7 transposition as described in Berger I et al, Nature Biotechnology 2004. Truncated mNPAS4 (1-329)/mARNT2 (1-481)/mnucTomato (monomeric nuclear) were recombined into MultiBAC by Tn7 transposition. All expression constructs Class I bHLH-PAS transcription factors were N-terminally tagged with a 6xHis-TEV tag immediately leading the endogenous start codon and class II ARNT or ARNT2 were untagged and expressed under the Polh and p10 baculoviral promoters.  Virus production and protein expression was carried out in Sf9 insect cells cultured in SF900III media essentially as described in Fitzgerald et al, Nature Methods 2006, with protein expression monitored by EYFP or nucTomato fluorescent protein expression.     Bacterial AhR expression and purification  BL21(DE3) (LysS) bacterial cells were co-transformed with pET16b-6xHis-TEV-hAhR(1-287) (AmpR) pAC28-hARNT(1-362) (KanR) (Ref). Bacteria was grown in Luria Broth to an OD600 of 0.6 and protein expressed was induced by the addition of 1mM IPTG at 16oC for 18hrs.     Insect or bacterially expressed proteins were purified by His-tag purification, followed by His tag removal by Tobacco Etch Virus (TEV) protease cleavage, ion-exchange, and size-exclusion chromatography. Protein purity was assessed by SDS-PAGE and coomassie staining and concentrations estimated by A280. Purified proteins were stored in a buffer containing (20mM Hepes pH 8.0, 300mM NaCl, 5% Glycerol, 10mM DTT), and flash frozen in LiN2, for long term storage at -80oC. This approach left untagged heterodimeric bHLH-PAS transcription factor complexes.     Expi293 mammalian expression and purification    C-terminal 3xFlag tagged full-length ARNT or ARNT plasmids (pEFIRES-Puro-hARNT-3xFlag or  pEFIRES-Puro-hARNT2-3xFlag) were transiently transfected into Expi293 cells (Life Technologies) grown in Expi293SF media using PEI Polyethylenimine (PolySciences). ~60hrs post-transfection, Expi293 cells were harvested by centrifugation, and washed with PBS. ARNT-3xFlag  or ARNT2-3xFlag were purified by Anti-Flag M2 resin and eluted with 3x Flag peptide (Sigma) and homodimers enriched using 100KDa mwco filters. The concentration and purity of ARNT-3xFlag or ARNT2-3xFlag was estimated by SDS-PAGE and commassie staining with BSA standards.

extract_protocol:
~250nM of purified transcription factor and 200nM (1.5μl of 5μM of DNA library (Random 18mer or FixedCore 18/22mer) , ~ 7x 1010 DNA molecules) of Round 0 library were incubated at room temperature in buffer containing ~20mM Tris-HCl pH 8.0, 3mM MgCl2, ~200-300mM NaCl, 8% Glycerol, 50 μg/ml polydI-dC, 0.2 mg/ml BSA. + 10mM DTT in a 30μl for 20mins. DNA was extracted, amplified as described in Riley et al 2014.
DNA isolated from SELEX Round 0 (initial Library), Round 1 and Round 2 (FixedCore 8NCGTG10N), or Round 3 (Random 18mer) were barcoded as described in Riley et al, Methods Mol Biol 2014 and illumina compatible adapters added by limited cycle PCR and cleaned up by PAGE purification. FixedCore 18/22mer SELEX samples from each round (Round 0, Round 1 , Round 2) of were quantified, pooled and run on separate lanes of a Hiseq2500 run. Random 18mer SELEX samples (Round 0 and Round 3) were barcoded by limited cycle PCR, quantified, pooled and sequenced on a NEXTSeq500 using a single end 1x75bp High Output mode.
SELEX-Seq Random 18mer –   GTTCAGAGTTCTACAGTCCGACGATC(N18)CTGTCTCTTATACACATCTCCG  SELEX-Seq FixedCore 18/22mer –  SELEX_18/22mer_Multiplex1: NPAS4/ARNT2  GTTCAGAGTTCTACAGTCCGACGATCTGGNNNNNNNNCGTGNNNNNNNNNNCCAgcTgTCGTATGCCGTCTTCTGCTTG  SELEX_18/22mer_Multiplex3:SIM1/ARNT2  GTTCAGAGTTCTACAGTCCGACGATCTGGNNNNNNNNCGTGNNNNNNNNNNCCAGAACTCGTATGCCGTCTTCTGCTTG  SELEX_18/22mer_Multiplex4:HIF2a/ARNT  GTTCAGAGTTCTACAGTCCGACGATCTGGNNNNNNNNCGTGNNNNNNNNNNCCAAGAGTCGTATGCCGTCTTCTGCTTG  SELEX_18/22mer_Multiplex5:AhR/ARNT  GTTCAGAGTTCTACAGTCCGACGATCTGGNNNNNNNNCGTGNNNNNNNNNNCCAACCTTCGTATGCCGTCTTCTGCTTG

tag: protein source

tag: protein


title: C2

city: Seoul

first: Dongyoun

last: Lee

address: 81, Irwon-ro, Gangnam-gu

country: South Korea

institute: Samsung Medical Center

library_source: transcriptomic

data_processing:
After cluster amplification of denatured templated, the prepared libraries were sequenced using Illumina Novaseq6000 (Illumina Technologies., San Diego, CA, USA)
The RNA-sequencing reads were mapped to the human reference genome (hg19) using the STAR (Spliced Transcripts Alignment to a Reference). Gene expression level quantification was performed using RSEM (RNA-seq by Expectation–Maximization).
Genome_build: hg19
Supplementary_files_format_and_content: Tab-delimited text file includes counts per million (CPM) for each sample

molecule: total RNA

organism: Homo sapiens

source_name: Nail matrix keratinocytes

growth_protocol: After removing the lower portion from nail matrix tissue, primary NMKs were harvested from nail matrix biopsy specimens. NMKs were isolated after 0.25% trypsin–EDTA treatment for 30 min and cultured in Keratinocyte Growth Media (KGM, Lonza) and maintained at 37 °C with 5% CO2. We checked no fibroblast growth under an inverted microscope. The cells which are passage three were plated in triplicate in six-well plates.

extract_protocol:
After 24h or 48 h of culture, NMK was treated with 1 ml of Trizol to isolate RNA for further bulk RNAseq experiments
Libraries preparation for 150bp paired-end sequencing was processed using the TruSeq Stranded Total RNA Sample Prep Kit with Ribo-Zero H/M/R (Illumina Technologies., San Diego, CA, USA), following the protocol. The integrity and overall quality of the total RNA was quantified using a Nanodrop 8000 UV- Vis spectrometer (NanoDrop Technologies Inc., Wilmington, DE, USA) and checked with a BioAnalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). After cluster amplification of denatured templated, the prepared libraries were sequenced using Illumina Novaseq6000 (Illumina Technologies., San Diego, CA, USA).

treatment_protocol: Recombinant human  BMP5  were treated at 500ng/ml and 100ng/ml in KGM without rhEGF, respectively.

tag: condition

value: Control

tag: cell source

value: Nail matrix


title: Y2_M2

city: Mölndal

first: Ramy

last: Elgendy

state: Göteborg

address: Pepparedsleden 1

country: Sweden

institute: AstraZeneca

data_processing:
Illumina sequencing and conventional base calling.
Sequenced reads were quality-controlled by FASTQC and trimmed by Trimmomatic v.0.36 for adaptor sequence (if present).
Clean sequences were mapped to the mm10 whole genome using STAR v2.7.
The uniquely-mapped reads aligned to exons were counted with HTSeq v.0.6.1, then tested for the presence of differentially expressed genes (DEGs) by the DESeq2 R package v.1.22.2.
Genome_build: mm10 (GRCm38)
Supplementary_files_format_and_content: *_htseq-count.txt: Tab-delimited text files with the raw counts for each sample.

organism: Mus musculus

source_name: Brain

extract_protocol:
Brains were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols.

tag: tissue

value: Brain

tag: age

value: 1 month

tag: genotype/variation

value: 3' Homozygous


title: Y2_WT

value: Wild type


title: Y1_WT


title: MOCK-WT-3

city: Taian

first: Ru Chang

last: Ren

address: Daizong

department: College of Life Sciences

country: China

institute: Shandong Agricultural University

data_processing:
We use internal software to filter reads, fllowed as:  1) Remove reads with adaptors;  2) Remove reads in which unknown bases(N) are more than 10%;  3) Remove low quality reads (we define the low quality read as the percentage of base which quality is lesser than 20 is greater than 50% in a read).
We use HISAT2 to perfrom genome mapping
we mapped clean reads to reference using Bowtie2, and then calculate gene expression level with RSEM.
Supplementary files format and content: tab-delimited text files include RPKM values for each Sample

organism: Zea mays

source_name: wild-type

extract_protocol:
Total RNA was isolated from leaves of WT and dsu seedling leaves through an Ultrapure RNA Kit (catalog no. CW0581, CWBIO, China).
RNA libraries were prepared for sequencing using standard Illumina protocols

value: leaf blade

tag: cell type

value: leaf cell

tag: genotype

value: wild-type

tag: treatment

value: mock


title: C1


title: MOCK-WT-2


title: MOCK-WT-1


title: FixedCore 8NCGTG10N Round 2

description: Library TGG8NCGTG10NCCA, barcodes; NPAS4_ARNT2 = GCTG, SIM1_ARNT2 = GAAC, HIF2a_ARNT = AGAG, AhR_ARNT = ACCT

growth_protocol: We purified full length human ARNT (1-774) or ARNT2 (1-717) homodimers from Expi293 cells, bHLH-PAS (PAS A and PAS B, N-terminus) heterdimers (human HIF1a(1-375)/ARNT(1-474), human HIF2a(1-353)/ARNT(1-474), human SIM1(1-348)/ARNT2(1-439) and mouse NPAS4(1-329)/ARNT2(1-481)) from baculovirus infected Sf9 insect cells or human AhR(1-287)/ARNT(1-364) from bacteria.    Baculoviral expression and purification  MultiBAC-LoxP-EYFP was generated by recombination of pUDCM-EYFP into the MultiBAC genome by Cre transposition as described Berger I et al, Nature Biotechnology 2004. With the exception of NPAS4 all truncated bHLH-PAS (A and B) heterodimers were incorporated into the MultiBAC-LoxP-EYFP baculoviral genome by pFBDM Tn7 transposition as described in Berger I et al, Nature Biotechnology 2004. Truncated mNPAS4 (1-329)/mARNT2 (1-481)/mnucTomato (monomeric nuclear) were recombined into MultiBAC by Tn7 transposition. All expression constructs Class I bHLH-PAS transcription factors were N-terminally tagged with a 6xHis-TEV tag immediately leading the endogenous start codon and class II ARNT or ARNT2 were untagged and expressed under the Polh and p10 baculoviral promoters.  Virus production and protein expression was carried out in Sf9 insect cells cultured in SF900III media essentially as described in Fitzgerald et al, Nature Methods 2006, with protein expression monitored by EYFP or nucTomato fluorescent protein expression.     Bacterial AhR expression and purification  BL21(DE3) (LysS) bacterial cells were co-transformed with pET16b-6xHis-TEV-hAhR(1-287) (AmpR) pAC28-hARNT(1-362) (KanR) (Ref). Bacteria was grown in Luria Broth to an OD600 of 0.6 and protein expressed was induced by the addition of 1mM IPTG at 16oC for 18hrs.     Insect or bacterially expressed proteins were purified by His-tag purification, followed by His tag removal by Tobacco Etch Virus (TEV) protease cleavage, ion-exchange, and size-exclusion chromatography. Protein purity was assessed by SDS-PAGE and coomassie staining and concentrations estimated by A280. Purified proteins were stored in a buffer containing (20mM Hepes pH 8.0, 300mM NaCl, 5% Glycerol, 10mM DTT), and flash frozen in LiN2, for long term storage at -80oC. This approach left untagged heterodimeric bHLH-PAS transcription factor complexes.     Expi293 mammalian expression and purification    C-terminal Twin strep tagged full-length ARNT or ARNT plasmids (pEFIRES-Puro-hARNT-2xStrep or  pEFIRES-Puro-hARNT2-2xStrep) were transiently transfected into Expi293 cells (Life Technologies) grown in Expi293SF media using PEI Polyethylenimine (PolySciences). ~60hrs post-transfection, Expi293 cells were harvested by centrifugation, and washed with PBS. ARNT-2xStrep  or ARNT2-2xStrep were purified by strep-tactin XT resin (IBA-LifeSciences) and homodimers enriched using 100KDa mwco filters. The concentration and purity of ARNT-2xStrep or ARNT2-2xStrep was estimated by SDS-PAGE and commassie staining with BSA standards.


title: FixedCore 8NCGTG10N Round 1


title: B4

value: BMP 100ng/ml treated


title: B6


title: B5


title: Y1_M1

value: 5' Homozygous


title: B3

value: BMP 500ng/ml treated


title: Old_Mix2

value: 10 months

value: Homozygous


title: FixedCore 8NCGTG10N Round 0


title: CDRH3_IGHV1-69_L54

city: Cambridge

first: Maya

last: Sangesland

state: MA

address: 400 Technology Square

country: USA

institute: Ragon Institute of MGH, MIT, and Harvard

data_processing:
The resulting paired end FASTQs were aligned using MiXCR (Bolotin et al., 2015).
Within this alignment step, we applied the following parameter: OvParameters.geneFeatureToAlign = {FR3Begin:Vend}, and constrained alignment to heavy chain matches only. We trimmed low sequence quality reads using the default settings within MiXCR.
Using custom scripts within immunarch (v0.5.5) tcR (Nazarov et al., 2015), we reconfigured the sequenced naïve IgM BCRs to generate frequency plots of CDRH3 length and assigned Kabat numbering for amino acid composition of 16aa length CDRH3s.
Assembly: IGMT
Supplementary files format and content: tab delimited txt file
Library strategy: BCR-seq

source_name: Splenocytes

extract_protocol:
Mouse spleens were removed and single cell suspensions were obtained using 70um cell strainer.
CDRH3 diversity within the transgenic mice was measured by deep sequencing the B cell receptors (BCRs) from antigen naïve IgM B cells in bulk according to previous methods (Sangesland et al., 2019; Trombetta et al., 2014). Briefly, BCR libraries were enriched from whole transcriptome amplification (WTA) products from B cells using the Smart-Seq2 protocol. Following WTA, antibody heavy chains were PCR amplified using a forward primer specific for the FR3 region within the VH gene of interest (IGHV1-69) and reverse primers against the heavy constant regions. These primers were attached to the Illumina P7 (FR3 region) and P5 (constant region). Heavy chain sequences were further amplified using step-out PCR with the Illumina sequencing adapters. The resulting PCR products (FR3 through CDRH3) were sequenced using the Illumina Miseq system (MiSeq Reagent Kit v2 500-cycle; Illumina, San Diego, US).

value: Splenocytes

tag: cell line

value: Primary Cells

value: B cells

value: Untreated:Naïve samples

tag: antibody chain

value: VH


title: Old_WT1

value: Brain


title: CDRH3_IGHV1-69_F54


title: SA_Haspin KD_ Chipseq

city: Barcelona

first: Oscar

last: Reina Garcia

state: Barcelona

address: C/Baldiri Reixac 10

department: Biostatistics and Bioinformatics

country: Spain

institute: IRB Barcelona

data_processing:
Illumina 50bp single end FAstQ samples were aligned against the dm3 genome using Bowtie 0.12.9 allowing for 2 mismatches per read and no multiple alignments.
TDF tracks for visualization were generated using IGVTools2
Public sample SRR1636785 (GSM1535999) which was generated and processed jointly with SA IP samples was downloaded using SRA Toolkit fastq-dump with options --split-files and read 1 (50bp) was aligned to the dm3 genome using the same software and parameters to be used as Input control.
Peaks between IP and Input sample were called using MACS 1.4.2 with default options and using -g dm.
Genome_build: dm3
Supplementary_files_format_and_content: Processed BED file contains identified peaks in IP samples according to MACS 1.4.2 format.

organism: Drosophila melanogaster

source_name: SA_Haspin KD, Kc167 cells

growth_protocol: Kc167 cells were grown on Schneider’s insect Medium (Sigma) supplemented with 100 μg/ml streptomycin (Gibco), 100 units/ml penicilin (Gibco) and 10% FBS (Gibco)

extract_protocol:
Cells were crosslinked with 1% formaldehyde for 10 min at room temperature and quenched with glycine. Nuclear lysates were sonicated to generate 200-500 bp fragments. Chromatin was precleared with Protein A or G Dynabeads at 4°C for 2 hours and incubated with antibody overnight at 4°C. Isolated chromatin was washed, eluted, reverse crosslinked and purified by standard methods.
Libraries were constructed using the standard protocol. Genomic fragments were end repaired (NEBNext End Repair Module), A-tailed by adding adenosine to the 3’ ends of fragment using Klenow fragment (3’ to 5’ exo minus, New England Biolabs), and adaptors were ligatedat room temperature for 1 hr with T4 DNA ligase (New England Biolabs). Libraries were amplified with Illumina primers using the KAPA SYBR FAST qPCR Master Mix.

treatment_protocol: Haspin depletion was carried out in Kc167 during 7 days with 10µg/ml of  Haspin dsRNAi every 48h.

value: Haspin KD

tag: chip antibody

value: anti-Stromalin (non-commercial, provided by Dale Dorsett)


title: B1

value: Nail matrix


title: ecm_yiep_3hp_2

city: Ulsan

first: Donghyuk

last: Kim

address: 50 UNIST-gil, Eonyang-eup, Ulju-gun

department: Department of Chemical Engineering

institute: UNIST

data_processing:
Sequencing: Illumina platform
Mapping: Sequence reads generated from RNA-seq were mapped onto the reference genome (NC_017635.1) using bowtie with the maximum insert size of 1000 bp, and 2 maximum mismatches after trimming 3 bp at 3’ ends
SAM: SAM files generated from bowtie.
Genome_build: Genome build: reference genome(NC_000913.2)
Supplementary_files_format_and_content: Tab-delimited text files in gff format which has 8 columns: sequence id, source(empty), feature (+/- strand), start position, end position, intensity score, strand(+/-), frame(.), attribute(.).

organism: Escherichia coli K-12

source_name: bacteria

growth_protocol: glycerol stocks of E. coli strains were inoculated into M9 minimal media with 0.2% (w/v) glucose. M9 minimal media was also supplemented with 1 ml trace element solution (100X) containing 1 g EDTA, 29 mg ZnSO4.7H2O, 198 mg MnCl2.4H2O, 254 mg CoCl2.6H2O, 13.4 mg CuCl2, and 147 mg CaCl2. The culture was incubated at 37 oC overnight with agitation, and then was used to inoculate the fresh media (1/200 dilution). The volume of the fresh media was 150 mL for each biological replicate. The fresh culture was incubated at 37 oC with agitation to the mid-log phase (OD600 ≈ 0.5).

extract_protocol:
Lysates were clarified from sonicated transcription factor-DNA complexes were isolated with myc antibody.
ChIP-exo experiment was performed following the procedures:  to identify each TF candidate binding maps in vivo, we isolated the DNA bound to each TF candidate from formaldehyde cross-linked E. coli cells by chromatin immunoprecipitation (ChIP) with the specific antibodies that specifically recognizes myc tag (9E10, Santa Cruz Biotechnology), and Dynabeads Pan Mouse IgG magnetic beads (Invitrogen) followed by stringent washings as described previously [42]. ChIP materials (chromatin-beads) were used to perform on-bead enzymatic reactions of the ChIP-exo method [11]. Briefly, the sheared DNA of chromatin-beads was repaired by the NEBNext End Repair Module (New England Biolabs) followed by the addition of a single dA overhang and ligation of the first adaptor (5’-phosphorylated) using dA-Tailing Module (New England Biolabs) and NEBNext Quick Ligation Module (New England Biolabs), respectively. Nick repair was performed by using PreCR Repair Mix (New England Biolabs). Lambda exonuclease- and RecJf exonuclease-treated chromatin was eluted from the beads and the protein-DNA cross-link was reversed by overnight incubation at 65oC. RNAs- and Proteins-removed DNA samples were used to perform primer extension and second adaptor ligation with following modifications. The DNA samples incubated for primer extension as described previously [12] were treated with dA-Tailing Module (New England Biolabs) and NEBNext Quick Ligation Module (New England Biolabs) for second adaptor ligation. The DNA sample purified by GeneRead Size Selection Kit (Qiagen) was enriched by polymerase chain reaction (PCR) using Phusion High-Fidelity DNA Polymerase (New England Biolabs). The amplified DNA samples were purified again by GeneRead Size Selection Kit (Qiagen) and quantified using Qubit dsDNA HS Assay Kit (Life Technologies). Quality of the DNA sample was checked by running Agilent High Sensitivity DNA Kit using Agilent 2100 Bioanalyzer (Agilent) before sequenced using HiSeq (Illumina) in accordance with the manufacturer’s instructions. Each modified step was also performed in accordance with the manufacturer’s instructions. ChIP-exo experiments were performed in biological duplicate.

tag: strain

tag: myc-tagging

value: yieP myc-tagging cells

tag: chip-exo antibody

value: c-myc(9E10) mouse monoclonal IgG1, sc-40, lot#J2413, SantaCruz Biotechnology


title: SAM01_0.02_probing_3_igg

status: Public on Jul 01 2022

type: protein

city: Rockville

first: Johannes

last: Goll

state: MD

address: 401 N Washington St

department: Bioinformatics Service Group

institute: The Emmes Company

hyb_protocol: Each serum sample was diluted in protein array blocking buffer (GVS, Sanford, ME) supplemented with E. coli lysate (GenScript, Piscataway, NJ) to a final concentration of 10 mg/mL, and pre-incubated at room temperature (RT) for 30 minutes. Concurrently, arrays were rehydrated in blocking buffer (without lysate) for 30 minutes. Blocking buffer was removed, and arrays were probed with pre-incubated serum samples using sealed chambers to ensure no cross-contamination of sample between pads. Arrays were incubated over-night at 4 °C with gentle agitation. Arrays were then washed at RT five times with TBS-0.05% Tween 20 (T-TBS), followed by incubation with QDot®-conjugated goat anti-human IgG/ IgA diluted 1:200 in blocking buffer for 2 hours at RT. After incubation in secondary antibodies, arrays were then washed three times with TTBS and once with water.

scan_protocol: Chips were air dried by centrifugation at 1,000 g for 5 minutes and scanned on a ArrayCamTM 400-S Microarray Imaging System from Grace Bio-Labs (Bend, OR) for QDot.

data_processing:
Spot and background intensities were measured using an annotated grid (gal) file. For ArrayCamTM a number of different settings were used to attempt to obtain readings. Microarray spot intensities were quantified using software ArrayCamTM (Grace Bio-Labs) utilizing automatic local background subtraction for each spot. The generated signal intensity values were considered raw values.
Prior to the analysis, systematic intensity differences in background reactivity as measured by phosphate buffered saline with tween 20 (PBST) were corrected. This was achieved by subtracting the median raw intensity signal for the 24 PBST intensity measurements for a certain array from the array’s non-PBST antigen intensities. Following background correction, for intensities < 1, an intensity value of 1 was imputed. Global outliers based on PCA and MDS plots were removed.

molecule: protein

source_name: serum

label_protocol: No labeling

extract_protocol: The serum samples originated from future-use consenting participants of parallel avian A(H5N1)/Indonesia/05/2005 influenza vaccine mix and match studies involving MF59® and AS03 adjuvant, also known as DMID Protocols 10-0016 and 10-0017.

treatment_protocol: Only the participants that received the 15 µg dose of H5 vaccine were selected for the microarray analysis; three timepoints from each participant were selected and represent baseline (day 0 or pre-vaccination), day 21 (post-first dose of vaccine), and day 42 (21 days post-second dose of vaccine).

tag: subject id

value: A/H5N1 HA + PBS

tag: day

value: Y

tag: dillusion


title: IP_2

type: RNA

city: Bellaterra

first: Elisenda

last: Sanz

address: M1/113 Facultat de Medicina

department: Institut de Neurociencies

institute: Universitat Autonoma de Barcelona

description:
Polysome RNA
Immunoprecipitate replicate 2  (pool)

hyb_protocol: Biotinilated cDNA was hybridized at 48ºC to MouseRef-8 v2 expression beadchips (Illumina) for 16h before washing and analyzing according to manufacturer’s directions.

scan_protocol: Signal was detected using a Beadarray Reader (Illumina)

data_processing: Data was analyzed for differential expression using the GenomeStudio data analysis software (Illumina) with average normalization.

label: Biotin

source_name: Immunoprecipitate replicate 2

label_protocol: RNA was amplified using the Ovation SL WTA system (Nugen) prior to biotinilation according to the EncoreIL biotinilation kit (Nugen).

extract_protocol: RNA was extracted using the RNeasy micro kit (Qiagen)

value: Calca neurons in the PBN


title: SAM01_0.01_probing_3_igg


title: SAM01_0.005_probing_3_igg


title: B2

tag: condition

value: BMP 500ng/ml treated


title: SAM10_0.00125_probing_3_igg


title: SAM10_0.01_probing_3_igg


title: SAM01_0.04_probing_3_igg


title: Y1_M2

value: 1 month

value: 5' Homozygous


title: ecm_yiep_3hp_1


title: SAM10_0.0025_probing_3_igg


title: SAM10_0.000625_probing_3_igg


title: SAM37_0.00125_probing_3_igg

value: N


title: SAM37_0.000625_probing_3_igg


title: Input_1

description: Input replicate 1 (pool)

source_name: Input replicate 1

value: All cells in the PBN


title: IP_1

description:
Polysome RNA
Immunoprecipitate replicate 1  (pool)

source_name: Immunoprecipitate replicate 1


title: Input_2

description: Input replicate 2  (pool)

source_name: Input replicate 2


title: SAM37_0.04_probing_3_igg


title: SAM52_0.02_probing_3_igg

value: A/H5N1 HA + AS03


title: SA_Chipseq

source_name: SA, Kc167 cells

value: WT


title: SAM52_0.0003125_probing_3_igg


title: SAM10_0.005_probing_3_igg


title: SAM10_0.02_probing_3_igg


title: SAM52_0.0025_probing_3_igg


title: SAM37_0.0003125_probing_3_igg


title: SAM53_0.00125_probing_3_igg

value: A/H5N1 HA + MF59


title: SAM37_0.01_probing_3_igg


title: SAM52_0.01_probing_3_igg


title: SAM53_0.0003125_probing_3_igg


title: SAM53_0.02_probing_3_igg


title: SAM53_0.000625_probing_3_igg


title: SAM52_0.04_probing_3_igg


title: SAM10_0.0003125_probing_3_igg


title: SAM37_0.005_probing_3_igg


title: SAM37_0.02_probing_3_igg


title: SAM37_0.0025_probing_3_igg


title: SAM52_0.00125_probing_3_igg


title: SAM53_0.01_probing_3_igg


title: SAM21_0.0025_probing_3_igg


title: SAM52_0.000625_probing_3_igg


title: SAM10_0.04_probing_3_igg


title: SAM21_0.0003125_probing_3_igg


title: SAM52_0.005_probing_3_igg


title: SAM21_0.005_probing_3_igg


title: SAM13_0.0025_probing_3_igg


title: SAM21_0.02_probing_3_igg


title: SAM53_0.005_probing_3_igg


title: SAM53_0.0025_probing_3_igg


title: SAM13_0.04_probing_3_igg


title: SAM53_0.04_probing_3_igg


title: SAM47_0.0003125_probing_3_igg


title: SAM21_0.00125_probing_3_igg


title: SAM47_0.000625_probing_3_igg


title: SAM21_0.04_probing_3_igg


title: SAM21_0.01_probing_3_igg


title: SAM13_0.0003125_probing_3_igg


title: SAM13_0.000625_probing_3_igg


title: SAM13_0.00125_probing_3_igg


title: SAM13_0.01_probing_3_igg


title: SAM11_0.0003125_probing_3_igg


title: SAM11_0.00125_probing_3_igg


title: SAM15_0.00125_probing_3_igg


title: SAM15_0.0003125_probing_3_igg


title: SAM47_0.00125_probing_3_igg


title: SAM13_0.02_probing_3_igg


title: SAM21_0.000625_probing_3_igg


title: SAM47_0.01_probing_3_igg


title: SAM47_0.0025_probing_3_igg


title: SAM15_0.02_probing_3_igg


title: SAM47_0.02_probing_3_igg


title: SAM47_0.005_probing_3_igg


title: SAM11_0.000625_probing_3_igg


title: SAM14_0.0003125_probing_3_igg


title: SAM47_0.04_probing_3_igg


title: SAM11_0.02_probing_3_igg


title: SAM14_0.00125_probing_3_igg


title: SAM11_0.0025_probing_3_igg


title: SAM11_0.005_probing_3_igg


title: SAM14_0.04_probing_3_igg


title: SAM11_0.04_probing_3_igg


title: SAM13_0.005_probing_3_igg


title: SAM11_0.01_probing_3_igg


title: SAM15_0.000625_probing_3_igg


title: SAM14_0.02_probing_3_igg


title: SAM48_0.000625_probing_3_igg


title: SAM15_0.0025_probing_3_igg


title: SAM15_0.005_probing_3_igg


title: SAM48_0.04_probing_3_igg


title: SAM15_0.01_probing_3_igg


title: SAM15_0.04_probing_3_igg


title: SAM14_0.000625_probing_3_igg


title: SAM12_0.0025_probing_3_igg


title: SAM12_0.02_probing_3_igg


title: SAM14_0.005_probing_3_igg


title: SAM48_0.0025_probing_3_igg


title: SAM14_0.01_probing_3_igg


title: SAM48_0.0003125_probing_3_igg


title: SAM44_0.04_probing_3_igg


title: SAM48_0.00125_probing_3_igg


title: SAM14_0.0025_probing_3_igg


title: SAM44_0.0003125_probing_3_igg


title: SAM48_0.005_probing_3_igg


title: SAM48_0.01_probing_3_igg


title: SAM48_0.02_probing_3_igg


title: SAM44_0.00125_probing_3_igg


title: SAM12_0.00125_probing_3_igg


title: SAM12_0.000625_probing_3_igg


title: SAM12_0.0003125_probing_3_igg


title: SAM44_0.0025_probing_3_igg


title: SAM12_0.01_probing_3_igg


title: SAM44_0.01_probing_3_igg


title: SAM08_0.0025_probing_3_igg


title: SAM44_0.02_probing_3_igg


title: SAM08_0.0003125_probing_3_igg


title: SAM12_0.04_probing_3_igg


title: SAM08_0.00125_probing_3_igg


title: SAM44_0.000625_probing_3_igg


title: SAM08_0.04_probing_3_igg


title: SAM08_0.000625_probing_3_igg


title: SAM08_0.005_probing_3_igg


title: SAM08_0.01_probing_3_igg


title: SAM08_0.02_probing_3_igg


title: SAM18_0.0003125_probing_3_igg


title: SAM18_0.02_probing_3_igg


title: SAM18_0.000625_probing_3_igg


title: SAM18_0.0025_probing_3_igg


title: SAM20_0.005_probing_3_igg


title: SAM20_0.0003125_probing_3_igg


title: SAM18_0.04_probing_3_igg


title: SAM12_0.005_probing_3_igg


title: SAM20_0.000625_probing_3_igg


title: SAM20_0.0025_probing_3_igg


title: SAM20_0.01_probing_3_igg


title: SAM20_0.04_probing_3_igg


title: SAM44_0.005_probing_3_igg


title: SAM45_0.00125_probing_3_igg


title: SAM18_0.01_probing_3_igg


title: SAM45_0.02_probing_3_igg


title: SAM20_0.00125_probing_3_igg


title: SAM45_0.0025_probing_3_igg


title: SAM45_0.0003125_probing_3_igg


title: SAM25_0.04_probing_3_igg


title: SAM18_0.005_probing_3_igg


title: SAM18_0.00125_probing_3_igg


title: SAM25_0.000625_probing_3_igg


title: SAM45_0.01_probing_3_igg


title: SAM25_0.0025_probing_3_igg


title: SAM25_0.00125_probing_3_igg


title: SAM20_0.02_probing_3_igg


title: SAM25_0.01_probing_3_igg


title: SAM25_0.005_probing_3_igg


title: SAM25_0.02_probing_3_igg


title: SAM58_0.0025_probing_3_igg


title: SAM45_0.000625_probing_3_igg


title: SAM58_0.00125_probing_3_igg


title: SAM58_0.005_probing_3_igg


title: SAM58_0.04_probing_3_igg


title: SAM58_0.01_probing_3_igg


title: SAM58_0.02_probing_3_igg


title: SAM45_0.005_probing_3_igg


title: SAM30_0.005_probing_3_igg


title: SAM30_0.000625_probing_3_igg


title: SAM30_0.0025_probing_3_igg


title: SAM30_0.00125_probing_3_igg


title: SAM30_0.0003125_probing_3_igg


title: SAM25_0.0003125_probing_3_igg


title: SAM45_0.04_probing_3_igg


title: SAM30_0.04_probing_3_igg


title: SAM34_0.005_probing_3_igg


title: SAM34_0.000625_probing_3_igg


title: SAM34_0.0003125_probing_3_igg


title: SAM34_0.00125_probing_3_igg


title: SAM30_0.01_probing_3_igg


title: SAM34_0.0025_probing_3_igg


title: SAM58_0.000625_probing_3_igg


title: SAM58_0.0003125_probing_3_igg


title: SAM34_0.04_probing_3_igg


title: SAM36_0.00125_probing_3_igg


title: SAM36_0.0003125_probing_3_igg


title: SAM34_0.02_probing_3_igg


title: SAM36_0.000625_probing_3_igg


title: SAM36_0.0025_probing_3_igg


title: SAM36_0.02_probing_3_igg


title: SAM36_0.01_probing_3_igg


title: SAM36_0.04_probing_3_igg


title: SAM07_0.0003125_probing_3_igg


title: SAM07_0.04_probing_3_igg


title: SAM07_0.01_probing_3_igg


title: SAM30_0.02_probing_3_igg


title: SAM29_0.00125_probing_3_igg


title: SAM29_0.000625_probing_3_igg


title: SAM07_0.000625_probing_3_igg


title: SAM07_0.0025_probing_3_igg


title: SAM29_0.0025_probing_3_igg


title: SAM07_0.00125_probing_3_igg


title: SAM29_0.04_probing_3_igg


title: SAM29_0.0003125_probing_3_igg


title: SAM07_0.005_probing_3_igg


title: SAM07_0.02_probing_3_igg


title: SAM29_0.005_probing_3_igg


title: SAM34_0.01_probing_3_igg


title: SAM46_0.005_probing_3_igg


title: SAM29_0.02_probing_3_igg


title: SAM36_0.005_probing_3_igg


title: SAM29_0.01_probing_3_igg


title: SAM46_0.0003125_probing_3_igg


title: SAM46_0.000625_probing_3_igg


title: SAM46_0.0025_probing_3_igg


title: SAM46_0.01_probing_3_igg


title: SAM46_0.00125_probing_3_igg


title: SAM46_0.02_probing_3_igg


title: SAM46_0.04_probing_3_igg


title: SAM16_0.04_probing_3_igg


title: SAM16_0.00125_probing_3_igg


title: SAM16_0.000625_probing_3_igg


title: SAM16_0.01_probing_3_igg


title: SAM16_0.02_probing_3_igg


title: SAM16_0.005_probing_3_igg


title: SAM24_0.0003125_probing_3_iga


title: SAM24_0.005_probing_3_iga


title: SAM24_0.00125_probing_3_iga


title: SAM24_0.04_probing_3_iga


title: SAM24_0.02_probing_3_iga


title: SAM24_0.0025_probing_3_iga


title: SAM24_0.000625_probing_3_iga


title: SAM24_0.01_probing_3_iga


title: SAM16_0.0025_probing_3_igg


title: SAM49_0.000625_probing_3_iga


title: SAM49_0.0003125_probing_3_iga


title: SAM49_0.005_probing_3_iga


title: SAM16_0.0003125_probing_3_igg


title: SAM49_0.01_probing_3_iga


title: SAM49_0.00125_probing_3_iga


title: SAM49_0.02_probing_3_iga


title: SAM49_0.04_probing_3_iga


title: SAM49_0.0025_probing_3_iga


title: SAM39_0.000625_probing_3_iga


title: SAM39_0.005_probing_3_iga


title: SAM39_0.01_probing_3_iga


title: SAM39_0.00125_probing_3_iga


title: SAM39_0.0003125_probing_3_iga


title: SAM39_0.02_probing_3_iga


title: SAM39_0.0025_probing_3_iga


title: SAM39_0.04_probing_3_iga


title: SAM31_0.000625_probing_3_iga


title: SAM31_0.0003125_probing_3_iga


title: SAM31_0.0025_probing_3_iga


title: SAM09_0.000625_probing_3_iga


title: SAM31_0.02_probing_3_iga


title: SAM09_0.04_probing_3_iga


title: SAM31_0.005_probing_3_iga


title: SAM31_0.04_probing_3_iga


title: SAM09_0.0003125_probing_3_iga


title: SAM09_0.0025_probing_3_iga


title: SAM09_0.005_probing_3_iga


title: SAM09_0.01_probing_3_iga


title: SAM50_0.00125_probing_3_iga


title: SAM50_0.0025_probing_3_iga


title: SAM50_0.04_probing_3_iga


title: SAM50_0.000625_probing_3_iga


title: SAM09_0.00125_probing_3_iga


title: SAM50_0.0003125_probing_3_iga


title: SAM09_0.02_probing_3_iga


title: SAM50_0.01_probing_3_iga


title: SAM43_0.0003125_probing_3_iga


title: SAM50_0.005_probing_3_iga


title: SAM50_0.02_probing_3_iga


title: SAM43_0.00125_probing_3_iga


title: SAM31_0.01_probing_3_iga


title: SAM43_0.000625_probing_3_iga


title: SAM43_0.0025_probing_3_iga


title: SAM31_0.00125_probing_3_iga


title: SAM43_0.005_probing_3_iga


title: SAM43_0.01_probing_3_iga


title: SAM43_0.04_probing_3_iga


title: SAM43_0.02_probing_3_iga


title: SAM54_0.0003125_probing_3_iga


title: SAM54_0.04_probing_3_iga


title: SAM54_0.005_probing_3_iga


title: SAM54_0.000625_probing_3_iga


title: SAM54_0.0025_probing_3_iga


title: SAM54_0.01_probing_3_iga


title: SAM54_0.00125_probing_3_iga


title: SAM54_0.02_probing_3_iga


title: SAM28_0.0003125_probing_3_iga


title: SAM28_0.00125_probing_3_iga


title: SAM28_0.02_probing_3_iga


title: SAM28_0.005_probing_3_iga


title: SAM51_0.01_probing_3_iga


title: SAM51_0.00125_probing_3_iga


title: SAM28_0.01_probing_3_iga


title: SAM28_0.04_probing_3_iga


title: SAM28_0.000625_probing_3_iga


title: SAM28_0.0025_probing_3_iga


title: SAM51_0.005_probing_3_iga


title: SAM51_0.0003125_probing_3_iga


title: SAM51_0.02_probing_3_iga


title: SAM51_0.000625_probing_3_iga


title: SAM51_0.0025_probing_3_iga


title: SAM38_0.005_probing_3_iga


title: SAM38_0.000625_probing_3_iga


title: SAM38_0.0003125_probing_3_iga


title: SAM38_0.0025_probing_3_iga


title: SAM38_0.01_probing_3_iga


title: SAM27_0.000625_probing_3_iga


title: SAM38_0.04_probing_3_iga


title: SAM27_0.0003125_probing_3_iga


title: SAM27_0.00125_probing_3_iga


title: SAM27_0.02_probing_3_iga


title: SAM51_0.04_probing_3_iga


title: SAM38_0.00125_probing_3_iga


title: SAM27_0.0025_probing_3_iga


title: SAM27_0.04_probing_3_iga


title: SAM55_0.0025_probing_3_iga


title: SAM55_0.01_probing_3_iga


title: SAM55_0.005_probing_3_iga


title: SAM55_0.04_probing_3_iga


title: SAM55_0.000625_probing_3_iga


title: SAM55_0.00125_probing_3_iga


title: SAM38_0.02_probing_3_iga


title: SAM19_0.0003125_probing_3_iga


title: SAM19_0.00125_probing_3_iga


title: SAM27_0.005_probing_3_iga


title: SAM55_0.02_probing_3_iga


title: SAM19_0.005_probing_3_iga


title: SAM19_0.02_probing_3_iga


title: SAM19_0.000625_probing_3_iga


title: SAM19_0.0025_probing_3_iga


title: SAM06_0.00125_probing_3_iga


title: SAM06_0.04_probing_3_iga


title: SAM06_0.0003125_probing_3_iga


title: SAM06_0.01_probing_3_iga


title: SAM06_0.02_probing_3_iga


title: SAM06_0.000625_probing_3_iga


title: SAM06_0.005_probing_3_iga


title: SAM05_0.000625_probing_3_iga


title: SAM05_0.005_probing_3_iga


title: SAM55_0.0003125_probing_3_iga


title: SAM05_0.00125_probing_3_iga


title: SAM19_0.04_probing_3_iga


title: SAM05_0.0003125_probing_3_iga


title: SAM05_0.02_probing_3_iga


title: SAM05_0.0025_probing_3_iga


title: SAM05_0.04_probing_3_iga


title: SAM59_0.00125_probing_3_iga


title: SAM59_0.000625_probing_3_iga


title: SAM59_0.02_probing_3_iga


title: SAM59_0.0003125_probing_3_iga


title: SAM59_0.005_probing_3_iga


title: SAM56_0.0003125_probing_3_iga


title: SAM59_0.04_probing_3_iga


title: SAM06_0.0025_probing_3_iga


title: SAM56_0.00125_probing_3_iga


title: SAM56_0.005_probing_3_iga


title: SAM56_0.02_probing_3_iga


title: SAM56_0.04_probing_3_iga


title: SAM56_0.000625_probing_3_iga


title: SAM56_0.0025_probing_3_iga


title: SAM05_0.01_probing_3_iga


title: SAM03_0.000625_probing_3_iga


title: SAM03_0.02_probing_3_iga


title: SAM03_0.0025_probing_3_iga


title: SAM03_0.0003125_probing_3_iga


title: SAM03_0.005_probing_3_iga


title: SAM59_0.01_probing_3_iga


title: SAM03_0.04_probing_3_iga


title: SAM26_0.000625_probing_3_iga


title: SAM26_0.04_probing_3_iga


title: SAM03_0.01_probing_3_iga


title: SAM59_0.0025_probing_3_iga


title: SAM26_0.0003125_probing_3_iga


title: SAM26_0.00125_probing_3_iga


title: SAM26_0.0025_probing_3_iga


title: SAM26_0.005_probing_3_iga


title: SAM26_0.01_probing_3_iga


title: SAM56_0.01_probing_3_iga


title: SAM33_0.00125_probing_3_iga


title: SAM33_0.0003125_probing_3_iga


title: SAM33_0.02_probing_3_iga


title: SAM03_0.00125_probing_3_iga


title: SAM33_0.005_probing_3_iga


title: SAM33_0.000625_probing_3_iga


title: SAM19_0.01_probing_3_iga


title: SAM33_0.01_probing_3_iga


title: SAM33_0.0025_probing_3_iga


title: SAM33_0.04_probing_3_iga


title: SAM42_0.02_probing_3_iga


title: SAM42_0.0003125_probing_3_iga


title: SAM42_0.00125_probing_3_iga


title: SAM26_0.02_probing_3_iga


title: SAM42_0.0025_probing_3_iga


title: SAM42_0.01_probing_3_iga


title: SAM42_0.000625_probing_3_iga


title: SAM40_0.0003125_probing_3_iga


title: SAM40_0.02_probing_3_iga


title: SAM40_0.00125_probing_3_iga


title: SAM40_0.01_probing_3_iga


title: SAM42_0.04_probing_3_iga


title: SAM40_0.005_probing_3_iga


title: SAM40_0.000625_probing_3_iga


title: SAM40_0.0025_probing_3_iga


title: SAM40_0.04_probing_3_iga


title: SAM23_0.0025_probing_3_iga


title: SAM23_0.005_probing_3_iga


title: SAM23_0.000625_probing_3_iga


title: SAM23_0.00125_probing_3_iga


title: SAM23_0.01_probing_3_iga


title: SAM23_0.02_probing_3_iga


title: SAM23_0.04_probing_3_iga


title: SAM22_0.04_probing_3_iga


title: SAM42_0.005_probing_3_iga


title: SAM23_0.0003125_probing_3_iga


title: SAM22_0.00125_probing_3_iga


title: SAM22_0.005_probing_3_iga


title: SAM22_0.01_probing_3_iga


title: SAM22_0.0003125_probing_3_iga


title: SAM22_0.000625_probing_3_iga


title: SAM22_0.02_probing_3_iga


title: SAM17_0.02_probing_3_iga


title: SAM17_0.00125_probing_3_iga


title: SAM17_0.005_probing_3_iga


title: SAM17_0.0003125_probing_3_iga


title: SAM17_0.01_probing_3_iga


title: SAM17_0.04_probing_3_iga


title: SAM32_0.000625_probing_3_iga


title: SAM17_0.000625_probing_3_iga


title: SAM17_0.0025_probing_3_iga


title: SAM32_0.02_probing_3_iga


title: SAM32_0.00125_probing_3_iga


title: SAM32_0.0003125_probing_3_iga


title: SAM32_0.01_probing_3_iga


title: SAM02_0.00125_probing_3_iga


title: SAM22_0.0025_probing_3_iga


title: SAM32_0.0025_probing_3_iga


title: SAM32_0.04_probing_3_iga


title: SAM02_0.005_probing_3_iga


title: SAM02_0.01_probing_3_iga


title: SAM02_0.0003125_probing_3_iga


title: SAM41_0.0025_probing_3_iga


title: SAM02_0.04_probing_3_iga


title: SAM41_0.00125_probing_3_iga


title: SAM41_0.0003125_probing_3_iga


title: SAM41_0.005_probing_3_iga


title: SAM41_0.04_probing_3_iga


title: SAM57_0.04_probing_3_iga


title: SAM57_0.00125_probing_3_iga


title: SAM57_0.0003125_probing_3_iga


title: SAM41_0.01_probing_3_iga


title: SAM32_0.005_probing_3_iga


title: SAM41_0.000625_probing_3_iga


title: SAM41_0.02_probing_3_iga


title: SAM57_0.005_probing_3_iga


title: SAM57_0.0025_probing_3_iga


title: SAM57_0.01_probing_3_iga


title: SAM02_0.02_probing_3_iga


title: SAM04_0.000625_probing_3_iga


title: SAM04_0.0003125_probing_3_iga


title: SAM04_0.01_probing_3_iga


title: SAM04_0.0025_probing_3_iga


title: SAM02_0.000625_probing_3_iga


title: SAM04_0.00125_probing_3_iga


title: SAM02_0.0025_probing_3_iga


title: SAM04_0.005_probing_3_iga


title: SAM04_0.02_probing_3_iga


title: SAM04_0.04_probing_3_iga


title: SAM60_0.02_probing_3_iga


title: SAM60_0.000625_probing_3_iga


title: SAM60_0.0003125_probing_3_iga


title: SAM57_0.000625_probing_3_iga


title: SAM35_0.00125_probing_3_iga


title: SAM35_0.000625_probing_3_iga


title: SAM60_0.0025_probing_3_iga


title: SAM35_0.02_probing_3_iga


title: SAM60_0.01_probing_3_iga


title: SAM60_0.04_probing_3_iga


title: SAM35_0.0025_probing_3_iga


title: SAM35_0.0003125_probing_3_iga


title: SAM57_0.02_probing_3_iga


title: SAM35_0.01_probing_3_iga


title: SAM01_0.0003125_probing_3_iga


title: SAM01_0.00125_probing_3_iga


title: SAM01_0.000625_probing_3_iga


title: SAM01_0.0025_probing_3_iga


title: SAM01_0.04_probing_3_iga


title: SAM01_0.01_probing_3_iga


title: SAM01_0.02_probing_3_iga


title: SAM10_0.0025_probing_3_iga


title: SAM10_0.0003125_probing_3_iga


title: SAM10_0.000625_probing_3_iga


title: SAM10_0.005_probing_3_iga


title: SAM60_0.005_probing_3_iga


title: SAM60_0.00125_probing_3_iga


title: SAM10_0.01_probing_3_iga


title: SAM10_0.04_probing_3_iga


title: SAM37_0.000625_probing_3_iga


title: SAM37_0.00125_probing_3_iga


title: SAM37_0.0003125_probing_3_iga


title: SAM35_0.005_probing_3_iga


title: SAM37_0.005_probing_3_iga


title: SAM37_0.0025_probing_3_iga


title: SAM01_0.005_probing_3_iga


title: SAM37_0.01_probing_3_iga


title: SAM52_0.0003125_probing_3_iga


title: SAM52_0.00125_probing_3_iga


title: SAM52_0.000625_probing_3_iga


title: SAM52_0.01_probing_3_iga


title: SAM52_0.0025_probing_3_iga


title: SAM35_0.04_probing_3_iga


title: SAM37_0.04_probing_3_iga


title: SAM10_0.00125_probing_3_iga


title: SAM52_0.02_probing_3_iga


title: SAM53_0.005_probing_3_iga


title: SAM53_0.0025_probing_3_iga


title: SAM52_0.005_probing_3_iga


title: SAM52_0.04_probing_3_iga


title: SAM53_0.0003125_probing_3_iga


title: SAM37_0.02_probing_3_iga


title: SAM53_0.01_probing_3_iga


title: SAM10_0.02_probing_3_iga


title: SAM21_0.00125_probing_3_iga


title: SAM21_0.005_probing_3_iga


title: SAM53_0.04_probing_3_iga


title: SAM53_0.02_probing_3_iga


title: SAM21_0.0025_probing_3_iga


title: SAM53_0.000625_probing_3_iga


title: SAM53_0.00125_probing_3_iga


title: SAM21_0.000625_probing_3_iga


title: SAM13_0.04_probing_3_iga


title: SAM13_0.00125_probing_3_iga


title: SAM21_0.02_probing_3_iga


title: SAM13_0.02_probing_3_iga


title: SAM13_0.0025_probing_3_iga


title: SAM21_0.04_probing_3_iga


title: SAM47_0.01_probing_3_iga


title: SAM47_0.00125_probing_3_iga


title: SAM47_0.0003125_probing_3_iga


title: SAM21_0.01_probing_3_iga


title: SAM13_0.01_probing_3_iga


title: SAM47_0.02_probing_3_iga


title: SAM13_0.000625_probing_3_iga


title: SAM13_0.0003125_probing_3_iga


title: SAM21_0.0003125_probing_3_iga


title: SAM47_0.005_probing_3_iga


title: SAM13_0.005_probing_3_iga


title: SAM47_0.0025_probing_3_iga


title: SAM11_0.0003125_probing_3_iga


title: SAM11_0.000625_probing_3_iga


title: SAM47_0.000625_probing_3_iga


title: SAM11_0.01_probing_3_iga


title: SAM47_0.04_probing_3_iga


title: SAM11_0.005_probing_3_iga


title: SAM11_0.02_probing_3_iga


title: SAM11_0.0025_probing_3_iga


title: SAM15_0.00125_probing_3_iga


title: SAM11_0.00125_probing_3_iga


title: SAM11_0.04_probing_3_iga


title: SAM15_0.0003125_probing_3_iga


title: SAM15_0.0025_probing_3_iga


title: SAM15_0.02_probing_3_iga


title: SAM15_0.005_probing_3_iga


title: SAM15_0.04_probing_3_iga


title: SAM27_0.01_probing_3_iga


title: SAM14_0.000625_probing_3_iga


title: SAM15_0.01_probing_3_iga


title: SAM14_0.00125_probing_3_iga


title: SAM14_0.04_probing_3_iga


title: SAM14_0.01_probing_3_iga


title: SAM14_0.005_probing_3_iga


title: SAM14_0.0003125_probing_3_iga


title: SAM48_0.000625_probing_3_iga


title: SAM14_0.02_probing_3_iga


title: SAM48_0.0003125_probing_3_iga


title: SAM48_0.00125_probing_3_iga


title: SAM48_0.005_probing_3_iga


title: SAM48_0.04_probing_3_iga


title: SAM15_0.000625_probing_3_iga


title: SAM12_0.00125_probing_3_iga


title: SAM48_0.0025_probing_3_iga


title: SAM12_0.02_probing_3_iga


title: SAM48_0.02_probing_3_iga


title: SAM48_0.01_probing_3_iga


title: SAM12_0.0003125_probing_3_iga


title: SAM12_0.0025_probing_3_iga


title: SAM12_0.005_probing_3_iga


title: SAM12_0.04_probing_3_iga


title: SAM12_0.000625_probing_3_iga


title: SAM44_0.04_probing_3_iga


title: SAM14_0.0025_probing_3_iga


title: SAM12_0.01_probing_3_iga


title: SAM44_0.0003125_probing_3_iga


title: SAM44_0.00125_probing_3_iga


title: SAM44_0.000625_probing_3_iga


title: SAM44_0.01_probing_3_iga


title: SAM44_0.0025_probing_3_iga


title: SAM44_0.005_probing_3_iga


title: SAM08_0.0003125_probing_3_iga


title: SAM44_0.02_probing_3_iga


title: SAM08_0.0025_probing_3_iga


title: SAM08_0.00125_probing_3_iga


title: SAM08_0.000625_probing_3_iga


title: SAM08_0.005_probing_3_iga


title: SAM08_0.01_probing_3_iga


title: SAM08_0.04_probing_3_iga


title: SAM08_0.02_probing_3_iga


title: SAM18_0.0003125_probing_3_iga


title: SAM18_0.000625_probing_3_iga


title: SAM18_0.0025_probing_3_iga


title: SAM20_0.0003125_probing_3_iga


title: SAM18_0.00125_probing_3_iga


title: SAM18_0.01_probing_3_iga


title: SAM18_0.02_probing_3_iga


title: SAM20_0.005_probing_3_iga


title: SAM18_0.04_probing_3_iga


title: SAM20_0.00125_probing_3_iga


title: SAM20_0.01_probing_3_iga


title: SAM20_0.000625_probing_3_iga


title: SAM20_0.0025_probing_3_iga


title: SAM45_0.00125_probing_3_iga


title: SAM20_0.02_probing_3_iga


title: SAM45_0.0003125_probing_3_iga


title: SAM20_0.04_probing_3_iga


title: SAM45_0.000625_probing_3_iga


title: SAM45_0.02_probing_3_iga


title: SAM18_0.005_probing_3_iga


title: SAM45_0.0025_probing_3_iga


title: SAM45_0.01_probing_3_iga


title: SAM45_0.005_probing_3_iga


title: SAM45_0.04_probing_3_iga


title: SAM25_0.04_probing_3_iga


title: SAM25_0.000625_probing_3_iga


title: SAM25_0.0025_probing_3_iga


title: SAM25_0.00125_probing_3_iga


title: SAM25_0.01_probing_3_iga


title: SAM25_0.005_probing_3_iga


title: SAM25_0.0003125_probing_3_iga


title: SAM58_0.0003125_probing_3_iga


title: SAM58_0.000625_probing_3_iga


title: SAM25_0.02_probing_3_iga


title: SAM58_0.005_probing_3_iga


title: SAM58_0.04_probing_3_iga


title: SAM58_0.00125_probing_3_iga


title: SAM58_0.01_probing_3_iga


title: SAM30_0.005_probing_3_iga


title: SAM30_0.000625_probing_3_iga


title: SAM30_0.0003125_probing_3_iga


title: SAM30_0.00125_probing_3_iga


title: SAM30_0.01_probing_3_iga


title: SAM30_0.0025_probing_3_iga


title: SAM30_0.02_probing_3_iga


title: SAM34_0.0003125_probing_3_iga


title: SAM34_0.000625_probing_3_iga


title: SAM34_0.005_probing_3_iga


title: SAM30_0.04_probing_3_iga


title: SAM34_0.00125_probing_3_iga


title: SAM34_0.02_probing_3_iga


title: SAM34_0.01_probing_3_iga


title: SAM34_0.0025_probing_3_iga


title: SAM36_0.000625_probing_3_iga


title: SAM36_0.0003125_probing_3_iga


title: SAM58_0.0025_probing_3_iga


title: SAM36_0.00125_probing_3_iga


title: SAM36_0.0025_probing_3_iga


title: SAM36_0.01_probing_3_iga


title: SAM36_0.02_probing_3_iga


title: SAM34_0.04_probing_3_iga


title: SAM58_0.02_probing_3_iga


title: SAM36_0.04_probing_3_iga


title: SAM36_0.005_probing_3_iga


title: SAM07_0.00125_probing_3_iga


title: SAM07_0.04_probing_3_iga


title: SAM07_0.000625_probing_3_iga


title: SAM07_0.0025_probing_3_iga


title: SAM29_0.0003125_probing_3_iga


title: SAM07_0.01_probing_3_iga


title: SAM07_0.02_probing_3_iga


title: SAM29_0.0025_probing_3_iga


title: SAM29_0.00125_probing_3_iga


title: SAM29_0.01_probing_3_iga


title: SAM29_0.005_probing_3_iga


title: SAM29_0.02_probing_3_iga


title: SAM46_0.000625_probing_3_iga


title: SAM46_0.00125_probing_3_iga


title: SAM46_0.005_probing_3_iga


title: SAM46_0.0025_probing_3_iga


title: SAM07_0.0003125_probing_3_iga


title: SAM46_0.01_probing_3_iga


title: SAM07_0.005_probing_3_iga


title: SAM44_r50_probing_4_iga

tag: blocking


title: SAM44_m50_probing_4_iga

value: Mini HA -50ug/ml


title: SAM69_m10_probing_4_iga

value: Mini HA-10ug/ml


title: SAM29_m50_probing_4_iga


title: SAM69_r10_probing_4_iga


title: SAM69_m50_probing_4_iga


title: SAM29_0.000625_probing_3_iga


title: SAM44_r10_probing_4_iga


title: SAM29_r10_probing_4_iga


title: SAM44_nop_probing_4_iga

value: no peptide


title: SAM29_nop_probing_4_iga


title: SAM29_0.04_probing_3_iga


title: SAM29_r50_probing_4_iga


title: SAM76_nop_probing_4_iga


title: SAM76_r10_probing_4_iga


title: SAM29_m10_probing_4_iga


title: SAM76_m50_probing_4_iga


title: SAM46_0.0003125_probing_3_iga


title: SAM69_r50_probing_4_iga


title: SAM76_r50_probing_4_iga


title: SAM76_m10_probing_4_iga


title: SAM87_m50_probing_4_iga


title: SAM87_r10_probing_4_iga


title: SAM87_nop_probing_4_iga


title: SAM87_r50_probing_4_iga


title: SAM58_r10_probing_4_iga


title: SAM44_m10_probing_4_iga


title: SAM87_m10_probing_4_iga


title: SAM58_m50_probing_4_iga


title: SAM58_nop_probing_4_iga


title: SAM58_m10_probing_4_iga


title: SAM58_r50_probing_4_iga


title: SAM79_m10_probing_4_iga


title: SAM79_nop_probing_4_iga


title: SAM79_m50_probing_4_iga


title: SAM43_m10_probing_4_iga


title: SAM43_nop_probing_4_iga


title: SAM10_r10_probing_4_iga


title: SAM79_r50_probing_4_iga


title: SAM43_m50_probing_4_iga


title: SAM43_r10_probing_4_iga


title: SAM43_r50_probing_4_iga


title: SAM10_m50_probing_4_iga


title: SAM10_m10_probing_4_iga


title: SAM10_nop_probing_4_iga


title: SAM82_m10_probing_4_iga


title: SAM10_r50_probing_4_iga


title: SAM82_r50_probing_4_iga


title: SAM82_r10_probing_4_iga


title: SAM82_m50_probing_4_iga


title: SAM78_nop_probing_4_iga


title: SAM78_r10_probing_4_iga


title: SAM78_m50_probing_4_iga


title: SAM79_r10_probing_4_iga


title: SAM78_r50_probing_4_iga


title: SAM56_nop_probing_4_iga


title: SAM78_m10_probing_4_iga


title: SAM56_m50_probing_4_iga


title: SAM56_r50_probing_4_iga


title: SAM56_r10_probing_4_iga


title: SAM52_r50_probing_4_iga


title: SAM52_r10_probing_4_iga


title: SAM56_m10_probing_4_iga


title: SAM52_m50_probing_4_iga


title: SAM22_r50_probing_4_iga


title: SAM52_nop_probing_4_iga


title: SAM52_m10_probing_4_iga


title: SAM08_m10_probing_4_iga


title: SAM08_m50_probing_4_iga


title: SAM08_r50_probing_4_iga


title: SAM22_nop_probing_4_iga


title: SAM22_r10_probing_4_iga


title: SAM82_nop_probing_4_iga


title: SAM22_m50_probing_4_iga


title: SAM22_m10_probing_4_iga


title: SAM31_r10_probing_4_iga


title: SAM31_m10_probing_4_iga


title: SAM31_r50_probing_4_iga


title: SAM31_m50_probing_4_iga


title: SAM31_nop_probing_4_iga


title: SAM25_m50_probing_4_iga


title: SAM25_r50_probing_4_iga


title: SAM25_r10_probing_4_iga


title: SAM23_nop_probing_4_iga


title: SAM25_m10_probing_4_iga


title: SAM08_r10_probing_4_iga


title: SAM08_nop_probing_4_iga


title: SAM23_m50_probing_4_iga


title: SAM23_m10_probing_4_iga


title: SAM23_r50_probing_4_iga


title: SAM64_nop_probing_4_iga


title: SAM23_r10_probing_4_iga


title: SAM64_m50_probing_4_iga


title: SAM42_m10_probing_4_iga


title: SAM46_r50_probing_4_iga


title: SAM42_r10_probing_4_iga


title: SAM46_r10_probing_4_iga


title: SAM42_r50_probing_4_iga


title: SAM42_nop_probing_4_iga


title: SAM59_m50_probing_4_iga


title: SAM25_nop_probing_4_iga


title: SAM64_r10_probing_4_iga


title: SAM64_r50_probing_4_iga


title: SAM46_nop_probing_4_iga


title: SAM42_m50_probing_4_iga


title: SAM59_r10_probing_4_iga


title: SAM59_m10_probing_4_iga


title: SAM63_r50_probing_4_iga


title: SAM46_m50_probing_4_iga


title: SAM63_nop_probing_4_iga


title: SAM63_r10_probing_4_iga


title: SAM63_m50_probing_4_iga


title: SAM46_m10_probing_4_iga


title: SAM63_m10_probing_4_iga


title: SAM13_nop_probing_4_iga


title: SAM13_r10_probing_4_iga


title: SAM64_m10_probing_4_iga


title: SAM13_m50_probing_4_iga


title: SAM40_m10_probing_4_iga


title: SAM13_r50_probing_4_iga


title: SAM89_r10_probing_4_iga


title: SAM40_m50_probing_4_iga


title: SAM59_r50_probing_4_iga


title: SAM59_nop_probing_4_iga


title: SAM89_m10_probing_4_iga


title: SAM40_nop_probing_4_iga


title: SAM89_r50_probing_4_iga


title: SAM34_r50_probing_4_iga


title: SAM89_nop_probing_4_iga


title: SAM34_nop_probing_4_iga


title: SAM34_r10_probing_4_iga


title: SAM34_m10_probing_4_iga


title: SAM05_r50_probing_4_iga


title: SAM34_m50_probing_4_iga


title: SAM05_nop_probing_4_iga


title: SAM05_m50_probing_4_iga


title: SAM05_m10_probing_4_iga


title: SAM13_m10_probing_4_iga


title: SAM19_m50_probing_4_iga


title: SAM19_nop_probing_4_iga


title: SAM19_r50_probing_4_iga


title: SAM05_r10_probing_4_iga


title: SAM19_r10_probing_4_iga


title: SAM40_r50_probing_4_iga


title: SAM14_m50_probing_4_iga


title: SAM40_r10_probing_4_iga


title: SAM19_m10_probing_4_iga


title: SAM21_m50_probing_4_iga


title: SAM14_m10_probing_4_iga


title: SAM21_r50_probing_4_iga


title: SAM89_m50_probing_4_iga


title: SAM75_m50_probing_4_iga


title: SAM21_nop_probing_4_iga


title: SAM75_r50_probing_4_iga


title: SAM21_r10_probing_4_iga


title: SAM75_m10_probing_4_iga


title: SAM75_r10_probing_4_iga


title: SAM20_r10_probing_4_iga


title: SAM20_m50_probing_4_iga


title: SAM75_nop_probing_4_iga


title: SAM21_m10_probing_4_iga


title: SAM20_m10_probing_4_iga


title: SAM20_nop_probing_4_iga


title: SAM14_nop_probing_4_iga


title: SAM39_r10_probing_4_iga


title: SAM66_nop_probing_4_iga


title: SAM39_m50_probing_4_iga


title: SAM39_r50_probing_4_iga


title: SAM39_m10_probing_4_iga


title: SAM20_r50_probing_4_iga


title: SAM14_r10_probing_4_iga


title: SAM39_nop_probing_4_iga


title: SAM14_r50_probing_4_iga


title: SAM66_r10_probing_4_iga


title: SAM66_m50_probing_4_iga


title: SAM66_r50_probing_4_iga


title: SAM12_r10_probing_4_iga


title: SAM66_m10_probing_4_iga


title: SAM73_m50_probing_4_iga


title: SAM73_r10_probing_4_iga


title: SAM12_nop_probing_4_iga


title: SAM12_m10_probing_4_iga


title: SAM28_r50_probing_4_iga


title: SAM73_r50_probing_4_iga


title: SAM12_r50_probing_4_iga


title: SAM28_m50_probing_4_iga


title: SAM73_nop_probing_4_iga


title: SAM88_m50_probing_4_iga


title: SAM28_nop_probing_4_iga


title: SAM28_r10_probing_4_iga


title: SAM28_m10_probing_4_iga


title: SAM88_nop_probing_4_iga


title: SAM88_m10_probing_4_iga


title: SAM88_r50_probing_4_iga


title: SAM09_m50_probing_4_iga


title: SAM12_m50_probing_4_iga


title: SAM88_r10_probing_4_iga


title: SAM07_r10_probing_4_iga


title: SAM07_r50_probing_4_iga


title: SAM09_nop_probing_4_iga


title: SAM09_m10_probing_4_iga


title: SAM07_m50_probing_4_iga


title: SAM09_r50_probing_4_iga


title: SAM09_r10_probing_4_iga


title: SAM74_m50_probing_4_iga


title: SAM07_nop_probing_4_iga


title: SAM07_m10_probing_4_iga


title: SAM41_m10_probing_4_iga


title: SAM74_r10_probing_4_iga


title: SAM74_r50_probing_4_iga


title: SAM73_m10_probing_4_iga


title: SAM41_r50_probing_4_iga


title: SAM74_nop_probing_4_iga


title: SAM41_nop_probing_4_iga


title: SAM41_r10_probing_4_iga


title: SAM80_m50_probing_4_iga


title: SAM80_r10_probing_4_iga


title: SAM80_r50_probing_4_iga


title: SAM80_m10_probing_4_iga


title: SAM74_m10_probing_4_iga


title: SAM80_nop_probing_4_iga


title: SAM27_r10_probing_4_iga


title: SAM27_m50_probing_4_iga


title: SAM17_nop_probing_4_iga


title: SAM27_nop_probing_4_iga


title: SAM17_m10_probing_4_iga


title: SAM27_m10_probing_4_iga


title: SAM17_r10_probing_4_iga


title: SAM71_m10_probing_4_iga


title: SAM71_r10_probing_4_iga


title: SAM71_r50_probing_4_iga


title: SAM17_m50_probing_4_iga


title: SAM27_r50_probing_4_iga


title: SAM41_m50_probing_4_iga


title: SAM17_r50_probing_4_iga


title: SAM37_m10_probing_4_iga


title: SAM71_m50_probing_4_iga


title: SAM37_m50_probing_4_iga


title: SAM37_r50_probing_4_iga


title: SAM37_nop_probing_4_iga


title: SAM30_r50_probing_4_iga


title: SAM37_r10_probing_4_iga


title: SAM30_m50_probing_4_iga


title: SAM81_m50_probing_4_iga


title: SAM30_m10_probing_4_iga


title: SAM81_r10_probing_4_iga


title: SAM81_r50_probing_4_iga


title: SAM81_nop_probing_4_iga


title: SAM81_m10_probing_4_iga


title: SAM65_m50_probing_4_iga


title: SAM65_m10_probing_4_iga


title: SAM33_r50_probing_4_iga


title: SAM33_nop_probing_4_iga


title: SAM16_r50_probing_4_iga


title: SAM65_r50_probing_4_iga


title: SAM30_r10_probing_4_iga


title: SAM16_nop_probing_4_iga


title: SAM83_nop_probing_4_iga


title: SAM83_r50_probing_4_iga


title: SAM83_m10_probing_4_iga


title: SAM33_r10_probing_4_iga


title: SAM16_r10_probing_4_iga


title: SAM33_m10_probing_4_iga


title: SAM65_nop_probing_4_iga


title: SAM65_r10_probing_4_iga


title: SAM38_m50_probing_4_iga


title: SAM38_m10_probing_4_iga


title: SAM38_r50_probing_4_iga


title: SAM16_m50_probing_4_iga


title: SAM30_nop_probing_4_iga


title: SAM33_m50_probing_4_iga


title: SAM71_nop_probing_4_iga


title: SAM11_r50_probing_4_iga


title: SAM11_m10_probing_4_iga


title: SAM18_r10_probing_4_iga


title: SAM16_m10_probing_4_iga


title: SAM83_m50_probing_4_iga


title: SAM83_r10_probing_4_iga


title: SAM85_nop_probing_4_iga


title: SAM85_m50_probing_4_iga


title: SAM36_nop_probing_4_iga


title: SAM85_r50_probing_4_iga


title: SAM38_nop_probing_4_iga


title: SAM38_r10_probing_4_iga


title: SAM85_m10_probing_4_iga


title: SAM36_r50_probing_4_iga


title: SAM11_m50_probing_4_iga


title: SAM36_m10_probing_4_iga


title: SAM11_nop_probing_4_iga


title: SAM36_r10_probing_4_iga


title: SAM36_m50_probing_4_iga


title: SAM70_r50_probing_4_iga


title: SAM11_r10_probing_4_iga


title: SAM70_r10_probing_4_iga


title: SAM18_r50_probing_4_iga


title: SAM18_nop_probing_4_iga


title: SAM18_m50_probing_4_iga


title: SAM18_m10_probing_4_iga


title: SAM70_m50_probing_4_iga


title: SAM70_nop_probing_4_iga


title: SAM84_r10_probing_4_iga


title: SAM84_nop_probing_4_iga


title: SAM84_r50_probing_4_iga


title: SAM84_m10_probing_4_iga


title: SAM84_m50_probing_4_iga


title: SAM85_r10_probing_4_iga


title: SAM03_r10_probing_4_iga


title: SAM03_m50_probing_4_iga


title: SAM35_m50_probing_4_iga


title: SAM35_r10_probing_4_iga


title: SAM03_m10_probing_4_iga


title: SAM35_m10_probing_4_iga


title: SAM03_nop_probing_4_iga


title: SAM70_m10_probing_4_iga


title: SAM35_r50_probing_4_iga


title: SAM35_nop_probing_4_iga


title: SAM24_nop_probing_4_iga


title: SAM15_r50_probing_4_iga


title: SAM51_r10_probing_4_iga


title: SAM24_r50_probing_4_iga


title: SAM51_m50_probing_4_iga


title: SAM15_r10_probing_4_iga


title: SAM15_m50_probing_4_iga


title: SAM24_r10_probing_4_iga


title: SAM15_nop_probing_4_iga


title: SAM51_m10_probing_4_iga


title: SAM51_nop_probing_4_iga


title: SAM24_m10_probing_4_iga


title: SAM54_m10_probing_4_iga


title: SAM03_r50_probing_4_iga


title: SAM24_m50_probing_4_iga


title: SAM54_r50_probing_4_iga


title: SAM54_r10_probing_4_iga


title: SAM54_nop_probing_4_iga


title: SAM51_r50_probing_4_iga


title: SAM54_m50_probing_4_iga


title: SAM02_r50_probing_4_iga


title: SAM02_m50_probing_4_iga


title: SAM02_nop_probing_4_iga


title: SAM02_m10_probing_4_iga


title: SAM02_r10_probing_4_iga


title: SAM04_nop_probing_4_iga


title: SAM15_m10_probing_4_iga


title: SAM04_r50_probing_4_iga


title: SAM62_m50_probing_4_iga


title: SAM62_m10_probing_4_iga


title: SAM62_r10_probing_4_iga


title: SAM57_m50_probing_4_iga


title: SAM04_r10_probing_4_iga


title: SAM57_r50_probing_4_iga


title: SAM48_r50_probing_4_iga


title: SAM57_r10_probing_4_iga


title: SAM04_m10_probing_4_iga


title: SAM48_m50_probing_4_iga


title: SAM57_m10_probing_4_iga


title: SAM48_m10_probing_4_iga


title: SAM48_r10_probing_4_iga


title: SAM26_r10_probing_4_iga


title: SAM62_r50_probing_4_iga


title: SAM62_nop_probing_4_iga


title: SAM26_r50_probing_4_iga


title: SAM26_nop_probing_4_iga


title: SAM26_m10_probing_4_iga


title: SAM04_m50_probing_4_iga


title: SAM86_m50_probing_4_iga


title: SAM86_r50_probing_4_iga


title: SAM86_m10_probing_4_iga


title: SAM72_m10_probing_4_iga


title: SAM86_r10_probing_4_iga


title: SAM26_m50_probing_4_iga


title: SAM72_r50_probing_4_iga


title: SAM72_nop_probing_4_iga


title: SAM86_nop_probing_4_iga


title: SAM57_nop_probing_4_iga


title: SAM49_m50_probing_4_iga


title: SAM72_m50_probing_4_iga


title: SAM49_m10_probing_4_iga


title: SAM60_r50_probing_4_iga


title: SAM72_r10_probing_4_iga


title: SAM49_r10_probing_4_iga


title: SAM60_nop_probing_4_iga


title: SAM48_nop_probing_4_iga


title: SAM60_m50_probing_4_iga


title: SAM49_nop_probing_4_iga


title: SAM60_r10_probing_4_iga


title: SAM49_r50_probing_4_iga


title: SAM90_nop_probing_4_iga


title: SAM90_m10_probing_4_iga


title: SAM60_m10_probing_4_iga


title: SAM90_r10_probing_4_iga


title: SAM47_r10_probing_4_iga


title: SAM90_m50_probing_4_iga


title: SAM47_nop_probing_4_iga


title: SAM47_m50_probing_4_iga


title: SAM47_m10_probing_4_iga


title: SAM06_m50_probing_4_iga


title: SAM06_r50_probing_4_iga


title: SAM06_r10_probing_4_iga


title: SAM67_m10_probing_4_iga


title: SAM06_m10_probing_4_iga


title: SAM67_r50_probing_4_iga


title: SAM06_nop_probing_4_iga


title: SAM67_nop_probing_4_iga


title: SAM67_m50_probing_4_iga


title: SAM55_r50_probing_4_iga


title: SAM55_r10_probing_4_iga


title: SAM55_nop_probing_4_iga


title: SAM55_m50_probing_4_iga


title: SAM77_m50_probing_4_iga


title: SAM77_nop_probing_4_iga


title: SAM90_r50_probing_4_iga


title: SAM77_r10_probing_4_iga


title: SAM24_0.0025_probing_3_igg


title: SAM55_m10_probing_4_iga


title: SAM77_m10_probing_4_iga


title: SAM47_r50_probing_4_iga


title: SAM24_0.04_probing_3_igg


title: SAM24_0.000625_probing_3_igg


title: SAM24_0.01_probing_3_igg


title: SAM24_0.005_probing_3_igg


title: SAM24_0.02_probing_3_igg


title: SAM49_0.0003125_probing_3_igg


title: SAM67_r10_probing_4_iga


title: SAM49_0.000625_probing_3_igg


title: SAM49_0.005_probing_3_igg


title: SAM49_0.00125_probing_3_igg


title: SAM49_0.0025_probing_3_igg


title: SAM49_0.04_probing_3_igg


title: SAM39_0.00125_probing_3_igg


title: SAM77_r50_probing_4_iga


title: SAM39_0.0003125_probing_3_igg


title: SAM39_0.0025_probing_3_igg


title: SAM31_0.000625_probing_3_igg


title: SAM24_0.00125_probing_3_igg


title: SAM24_0.0003125_probing_3_igg


title: SAM39_0.01_probing_3_igg


title: SAM39_0.000625_probing_3_igg


title: SAM39_0.04_probing_3_igg


title: SAM31_0.0003125_probing_3_igg


title: SAM39_0.02_probing_3_igg


title: SAM31_0.0025_probing_3_igg


title: SAM31_0.04_probing_3_igg


title: SAM31_0.005_probing_3_igg


title: SAM09_0.04_probing_3_igg


title: SAM31_0.00125_probing_3_igg


title: SAM49_0.02_probing_3_igg


title: SAM09_0.00125_probing_3_igg


title: SAM31_0.02_probing_3_igg


title: SAM09_0.005_probing_3_igg


title: SAM09_0.0025_probing_3_igg


title: SAM49_0.01_probing_3_igg


title: SAM09_0.0003125_probing_3_igg


title: SAM50_0.04_probing_3_igg


title: SAM09_0.02_probing_3_igg


title: SAM31_0.01_probing_3_igg


title: SAM09_0.01_probing_3_igg


title: SAM50_0.00125_probing_3_igg


title: SAM50_0.000625_probing_3_igg


title: SAM43_0.0003125_probing_3_igg


title: SAM50_0.02_probing_3_igg


title: SAM43_0.04_probing_3_igg


title: SAM43_0.005_probing_3_igg


title: SAM39_0.005_probing_3_igg


title: SAM43_0.000625_probing_3_igg


title: SAM43_0.00125_probing_3_igg


title: SAM54_0.005_probing_3_igg


title: SAM54_0.000625_probing_3_igg


title: SAM43_0.01_probing_3_igg


title: SAM54_0.0003125_probing_3_igg


title: SAM54_0.00125_probing_3_igg


title: SAM54_0.01_probing_3_igg


title: SAM28_0.005_probing_3_igg


title: SAM50_0.01_probing_3_igg


title: SAM28_0.0003125_probing_3_igg


title: SAM50_0.0025_probing_3_igg


title: SAM43_0.02_probing_3_igg


title: SAM28_0.0025_probing_3_igg


title: SAM54_0.04_probing_3_igg


title: SAM50_0.005_probing_3_igg


title: SAM54_0.02_probing_3_igg


title: SAM50_0.0003125_probing_3_igg


title: SAM28_0.000625_probing_3_igg


title: SAM28_0.02_probing_3_igg


title: SAM09_0.000625_probing_3_igg


title: SAM43_0.0025_probing_3_igg


title: SAM51_0.01_probing_3_igg


title: SAM51_0.0003125_probing_3_igg


title: SAM54_0.0025_probing_3_igg


title: SAM51_0.02_probing_3_igg


title: SAM51_0.00125_probing_3_igg


title: SAM51_0.005_probing_3_igg


title: SAM51_0.04_probing_3_igg


title: SAM51_0.000625_probing_3_igg


title: SAM38_0.000625_probing_3_igg


title: SAM38_0.02_probing_3_igg


title: SAM51_0.0025_probing_3_igg


title: SAM38_0.005_probing_3_igg


title: SAM28_0.00125_probing_3_igg


title: SAM38_0.0025_probing_3_igg


title: SAM38_0.04_probing_3_igg


title: SAM27_0.000625_probing_3_igg


title: SAM27_0.0003125_probing_3_igg


title: SAM27_0.02_probing_3_igg


title: SAM27_0.00125_probing_3_igg


title: SAM27_0.01_probing_3_igg


title: SAM27_0.04_probing_3_igg


title: SAM27_0.005_probing_3_igg


title: SAM55_0.01_probing_3_igg


title: SAM55_0.0025_probing_3_igg


title: SAM38_0.01_probing_3_igg


title: SAM55_0.005_probing_3_igg


title: SAM27_0.0025_probing_3_igg


title: SAM55_0.00125_probing_3_igg


title: SAM55_0.04_probing_3_igg


title: SAM55_0.000625_probing_3_igg


title: SAM19_0.04_probing_3_igg


title: SAM55_0.02_probing_3_igg


title: SAM55_0.0003125_probing_3_igg


title: SAM19_0.0003125_probing_3_igg


title: SAM19_0.005_probing_3_igg


title: SAM19_0.01_probing_3_igg


title: SAM38_0.00125_probing_3_igg


title: SAM38_0.0003125_probing_3_igg


title: SAM19_0.00125_probing_3_igg


title: SAM19_0.02_probing_3_igg


title: SAM19_0.0025_probing_3_igg


title: SAM06_0.00125_probing_3_igg


title: SAM06_0.04_probing_3_igg


title: SAM06_0.005_probing_3_igg


title: SAM06_0.02_probing_3_igg


title: SAM06_0.0003125_probing_3_igg


title: SAM06_0.01_probing_3_igg


title: SAM06_0.0025_probing_3_igg


title: SAM05_0.000625_probing_3_igg


title: SAM05_0.0003125_probing_3_igg


title: SAM28_0.04_probing_3_igg


title: SAM05_0.00125_probing_3_igg


title: SAM05_0.005_probing_3_igg


title: SAM05_0.02_probing_3_igg


title: SAM05_0.01_probing_3_igg


title: SAM59_0.01_probing_3_igg


title: SAM05_0.04_probing_3_igg


title: SAM59_0.00125_probing_3_igg


title: SAM59_0.0003125_probing_3_igg


title: SAM59_0.000625_probing_3_igg


title: SAM59_0.02_probing_3_igg


title: SAM05_0.0025_probing_3_igg


title: SAM59_0.0025_probing_3_igg


title: SAM56_0.0003125_probing_3_igg


title: SAM56_0.00125_probing_3_igg


title: SAM59_0.005_probing_3_igg


title: SAM59_0.04_probing_3_igg


title: SAM06_0.000625_probing_3_igg


title: SAM56_0.0025_probing_3_igg


title: SAM56_0.005_probing_3_igg


title: SAM56_0.01_probing_3_igg


title: SAM56_0.04_probing_3_igg


title: SAM19_0.000625_probing_3_igg


title: SAM56_0.02_probing_3_igg


title: SAM56_0.000625_probing_3_igg


title: SAM03_0.000625_probing_3_igg


title: SAM03_0.0003125_probing_3_igg


title: SAM03_0.02_probing_3_igg


title: SAM03_0.0025_probing_3_igg


title: SAM03_0.005_probing_3_igg


title: SAM03_0.01_probing_3_igg


title: SAM03_0.04_probing_3_igg


title: SAM26_0.00125_probing_3_igg


title: SAM26_0.000625_probing_3_igg


title: SAM26_0.04_probing_3_igg


title: SAM26_0.02_probing_3_igg


title: SAM26_0.0025_probing_3_igg


title: SAM26_0.01_probing_3_igg


title: SAM26_0.0003125_probing_3_igg


title: SAM33_0.00125_probing_3_igg


title: SAM33_0.0025_probing_3_igg


title: SAM33_0.0003125_probing_3_igg


title: SAM33_0.000625_probing_3_igg


title: SAM03_0.00125_probing_3_igg


title: SAM33_0.005_probing_3_igg


title: SAM33_0.02_probing_3_igg


title: SAM33_0.01_probing_3_igg


title: SAM42_0.005_probing_3_igg


title: SAM42_0.00125_probing_3_igg


title: SAM42_0.0025_probing_3_igg


title: SAM42_0.000625_probing_3_igg


title: SAM42_0.04_probing_3_igg


title: SAM42_0.02_probing_3_igg


title: SAM42_0.01_probing_3_igg


title: SAM42_0.0003125_probing_3_igg


title: SAM40_0.0003125_probing_3_igg


title: SAM40_0.02_probing_3_igg


title: SAM40_0.01_probing_3_igg


title: SAM40_0.005_probing_3_igg


title: SAM26_0.005_probing_3_igg


title: SAM23_0.000625_probing_3_igg


title: SAM40_0.000625_probing_3_igg


title: SAM40_0.0025_probing_3_igg


title: SAM40_0.00125_probing_3_igg


title: SAM23_0.0025_probing_3_igg


title: SAM40_0.04_probing_3_igg


title: SAM23_0.02_probing_3_igg


title: SAM23_0.0003125_probing_3_igg


title: SAM23_0.01_probing_3_igg


title: SAM23_0.04_probing_3_igg


title: SAM33_0.04_probing_3_igg


title: SAM22_0.04_probing_3_igg


title: SAM22_0.0025_probing_3_igg


title: SAM22_0.02_probing_3_igg


title: SAM22_0.01_probing_3_igg


title: SAM17_0.00125_probing_3_igg


title: SAM17_0.04_probing_3_igg


title: SAM17_0.02_probing_3_igg


title: SAM17_0.005_probing_3_igg


title: SAM23_0.00125_probing_3_igg


title: SAM22_0.005_probing_3_igg


title: SAM17_0.01_probing_3_igg


title: SAM22_0.000625_probing_3_igg


title: SAM32_0.00125_probing_3_igg


title: SAM32_0.01_probing_3_igg


title: SAM17_0.0003125_probing_3_igg


title: SAM02_0.005_probing_3_igg


title: SAM02_0.02_probing_3_igg


title: SAM02_0.000625_probing_3_igg


title: SAM02_0.00125_probing_3_igg


title: SAM32_0.0003125_probing_3_igg


title: SAM32_0.02_probing_3_igg


title: SAM17_0.000625_probing_3_igg


title: SAM17_0.0025_probing_3_igg


title: SAM28_0.01_probing_3_igg


title: SAM32_0.005_probing_3_igg


title: SAM32_0.000625_probing_3_igg


title: SAM22_0.0003125_probing_3_igg


title: SAM32_0.0025_probing_3_igg


title: SAM32_0.04_probing_3_igg


title: SAM02_0.0025_probing_3_igg


title: SAM02_0.01_probing_3_igg


title: SAM02_0.04_probing_3_igg


title: SAM57_0.0003125_probing_3_igg


title: SAM02_0.0003125_probing_3_igg


title: SAM57_0.04_probing_3_igg


title: SAM41_0.0025_probing_3_igg


title: SAM57_0.0025_probing_3_igg


title: SAM57_0.000625_probing_3_igg


title: SAM41_0.0003125_probing_3_igg


title: SAM57_0.02_probing_3_igg


title: SAM57_0.00125_probing_3_igg


title: SAM41_0.00125_probing_3_igg


title: SAM41_0.000625_probing_3_igg


title: SAM41_0.02_probing_3_igg


title: SAM04_0.0025_probing_3_igg


title: SAM41_0.01_probing_3_igg


title: SAM57_0.005_probing_3_igg


title: SAM04_0.005_probing_3_igg


title: SAM41_0.04_probing_3_igg


title: SAM57_0.01_probing_3_igg


title: SAM04_0.01_probing_3_igg


title: SAM41_0.005_probing_3_igg


title: SAM60_0.005_probing_3_igg


title: SAM60_0.000625_probing_3_igg


title: SAM60_0.0025_probing_3_igg


title: SAM04_0.00125_probing_3_igg


title: SAM04_0.000625_probing_3_igg


title: SAM04_0.02_probing_3_igg


title: SAM35_0.02_probing_3_igg


title: SAM35_0.000625_probing_3_igg


title: SAM04_0.0003125_probing_3_igg


title: SAM35_0.0003125_probing_3_igg


title: SAM60_0.0003125_probing_3_igg


title: SAM35_0.005_probing_3_igg


title: SAM60_0.04_probing_3_igg


title: SAM04_0.04_probing_3_igg


title: SAM60_0.00125_probing_3_igg


title: SAM60_0.02_probing_3_igg


title: SAM35_0.04_probing_3_igg


title: SAM01_0.000625_probing_3_igg


title: SAM35_0.00125_probing_3_igg


title: SAM35_0.01_probing_3_igg


title: SAM60_0.01_probing_3_igg


title: SAM16_0.01_probing_3_iga


title: SAM01_0.0003125_probing_3_igg


title: SAM16_0.00125_probing_3_iga


title: SAM16_0.0025_probing_3_iga


title: SAM01_0.0025_probing_3_igg


title: SAM16_0.005_probing_3_iga


title: SAM16_0.02_probing_3_iga


title: SAM46_0.02_probing_3_iga


title: SAM087_probing_2_igg


title: SAM379_probing_2_igg


title: SAM35_0.0025_probing_3_igg


title: SAM16_0.0003125_probing_3_iga


title: SAM331_probing_2_igg


title: SAM102_probing_2_igg


title: SAM01_0.00125_probing_3_igg


title: SAM46_0.04_probing_3_iga


title: SAM16_0.04_probing_3_iga


title: SAM317_probing_2_igg


title: SAM290_probing_2_igg


title: SAM340_probing_2_igg


title: SAM361_probing_2_igg


title: SAM23_0.005_probing_3_igg


title: SAM311_probing_2_igg


title: SAM16_0.000625_probing_3_iga


title: SAM22_0.00125_probing_3_igg


title: SAM256_probing_2_igg


title: SAM014_probing_2_igg


title: SAM344_probing_2_igg


title: SAM288_probing_2_igg


title: SAM004_probing_2_igg


title: SAM134_probing_2_igg


title: SAM119_probing_2_igg


title: SAM157_probing_2_igg


title: SAM207_probing_2_igg


title: SAM071_probing_2_igg


title: SAM267_probing_2_igg


title: SAM339_probing_2_igg


title: SAM077_probing_2_igg


title: SAM208_probing_2_igg


title: SAM156_probing_2_igg


title: SAM202_probing_2_igg


title: SAM322_probing_2_igg


title: SAM364_probing_2_igg


title: SAM178_probing_2_igg


title: SAM082_probing_2_igg


title: SAM047_probing_2_igg


title: SAM057_probing_2_igg


title: SAM053_probing_2_igg


title: SAM051_probing_2_igg


title: SAM060_probing_2_igg


title: SAM247_probing_2_igg


title: SAM327_probing_2_igg


title: SAM211_probing_2_igg


title: SAM237_probing_2_igg


title: SAM045_probing_2_igg


title: SAM274_probing_2_igg


title: SAM330_probing_2_igg


title: SAM216_probing_2_igg


title: SAM054_probing_2_igg


title: SAM280_probing_2_igg


title: SAM386_probing_2_igg


title: SAM342_probing_2_igg


title: SAM265_probing_2_igg


title: SAM188_probing_2_igg


title: SAM347_probing_2_igg


title: SAM007_probing_2_igg


title: SAM277_probing_2_igg


title: SAM155_probing_2_igg


title: SAM122_probing_2_igg


title: SAM106_probing_2_igg


title: SAM363_probing_2_igg


title: SAM172_probing_2_igg


title: SAM169_probing_2_igg


title: SAM149_probing_2_igg


title: SAM182_probing_2_igg


title: SAM230_probing_2_igg


title: SAM109_probing_2_igg


title: SAM375_probing_2_igg


title: SAM293_probing_2_igg


title: SAM214_probing_2_igg


title: SAM291_probing_2_igg


title: SAM008_probing_2_igg


title: SAM114_probing_2_igg


title: SAM061_probing_2_igg


title: SAM031_probing_2_igg


title: SAM142_probing_2_igg


title: SAM204_probing_2_igg


title: SAM173_probing_2_igg


title: SAM100_probing_2_igg


title: SAM328_probing_2_igg


title: SAM153_probing_2_igg


title: SAM115_probing_2_igg


title: SAM390_probing_2_igg


title: SAM196_probing_2_igg


title: SAM148_probing_2_igg


title: SAM192_probing_2_igg


title: SAM069_probing_2_igg


title: SAM137_probing_2_igg


title: SAM348_probing_2_igg


title: SAM329_probing_2_igg


title: SAM326_probing_2_igg


title: SAM226_probing_2_igg


title: SAM076_probing_2_igg


title: SAM372_probing_2_igg


title: SAM089_probing_2_igg


title: SAM095_probing_2_igg


title: SAM030_probing_2_igg


title: SAM309_probing_2_igg


title: SAM133_probing_2_igg


title: SAM338_probing_2_igg


title: SAM123_probing_2_igg


title: SAM086_probing_2_igg


title: SAM096_probing_2_igg


title: SAM300_probing_2_igg


title: SAM382_probing_2_igg


title: SAM236_probing_2_igg


title: SAM183_probing_2_igg


title: SAM366_probing_2_igg


title: SAM320_probing_2_igg


title: SAM117_probing_2_igg


title: SAM298_probing_2_igg


title: SAM070_probing_2_igg


title: SAM308_probing_2_igg


title: SAM046_probing_2_igg


title: SAM065_probing_2_igg


title: SAM201_probing_2_igg


title: SAM385_probing_2_igg


title: SAM279_probing_2_igg


title: SAM285_probing_2_igg


title: SAM003_probing_2_igg


title: SAM360_probing_2_igg


title: SAM166_probing_2_igg


title: SAM205_probing_2_igg


title: SAM367_probing_2_igg


title: SAM282_probing_2_igg


title: SAM055_probing_2_igg


title: SAM264_probing_2_igg


title: SAM243_probing_2_igg


title: SAM048_probing_2_igg


title: SAM104_probing_2_igg


title: SAM234_probing_2_igg


title: SAM286_probing_2_igg


title: SAM321_probing_2_igg


title: SAM110_probing_2_igg


title: SAM281_probing_2_igg


title: SAM038_probing_2_igg


title: SAM189_probing_2_igg


title: SAM093_probing_2_igg


title: SAM075_probing_2_igg


title: SAM359_probing_2_igg


title: SAM185_probing_2_igg


title: SAM105_probing_2_igg


title: SAM349_probing_2_igg


title: SAM171_probing_2_igg


title: SAM128_probing_2_igg


title: SAM090_probing_2_igg


title: SAM132_probing_2_igg


title: SAM258_probing_2_igg


title: SAM197_probing_2_igg


title: SAM356_probing_2_igg


title: SAM350_probing_2_igg


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title: SAM301_probing_2_iga


title: SAM222_probing_2_iga


title: SAM333_probing_2_iga


title: SAM140_probing_2_iga


title: SAM272_probing_2_iga


title: SAM344_probing_1_igg


title: SAM361_probing_1_igg


title: SAM379_probing_1_igg


title: SAM102_probing_1_igg


title: SAM059_probing_2_iga


title: SAM221_probing_2_iga


title: SAM077_probing_1_igg


title: SAM331_probing_1_igg


title: SAM290_probing_1_igg


title: SAM004_probing_1_igg


title: SAM178_probing_1_igg


title: SAM340_probing_1_igg


title: SAM317_probing_1_igg


title: SAM288_probing_1_igg


title: SAM157_probing_1_igg


title: SAM256_probing_1_igg


title: SAM014_probing_1_igg


title: SAM134_probing_1_igg


title: SAM087_probing_1_igg


title: SAM339_probing_1_igg


title: SAM071_probing_1_igg


title: SAM364_probing_1_igg


title: SAM347_probing_1_igg


title: SAM322_probing_1_igg


title: SAM267_probing_1_igg


title: SAM202_probing_1_igg


title: SAM208_probing_1_igg


title: SAM207_probing_1_igg


title: SAM156_probing_1_igg


title: SAM057_probing_1_igg


title: SAM237_probing_1_igg


title: SAM051_probing_1_igg


title: SAM311_probing_1_igg


title: SAM082_probing_1_igg


title: SAM274_probing_1_igg


title: SAM119_probing_1_igg


title: SAM045_probing_1_igg


title: SAM007_probing_1_igg


title: SAM053_probing_1_igg


title: SAM060_probing_1_igg


title: SAM327_probing_1_igg


title: SAM247_probing_1_igg


title: SAM386_probing_1_igg


title: SAM330_probing_1_igg


title: SAM216_probing_1_igg


title: SAM172_probing_1_igg


title: SAM177_probing_2_iga


title: SAM211_probing_1_igg


title: SAM342_probing_1_igg


title: SAM277_probing_1_igg


title: SAM047_probing_1_igg


title: SAM265_probing_1_igg


title: SAM125_probing_2_iga


title: SAM008_probing_1_igg


title: SAM375_probing_1_igg


title: SAM182_probing_1_igg