title: ASF1A_Flag_ChIPseq_rep-1

description: ChIP-seq data of ASF1A flag replicate 1


title: Con1

status: Public on Oct 27 2022

type: SRA

city: Beijing

first: Shuang

last: Li

state: Beijing

address: Biomedical engineering

department: Biomedical engineering

country: China

institute: Beihang university

library_source: transcriptomic

data_processing:
Sequencing adapters removal performed with Trimmomatic (v0.32)
Reads in the size range of 18-50 nucleotides were selected and used for downstream analysis
Mapped against mature miRNAs in the mirBase (version 21) using BWA tool (command bwa aln; v0.7.5a)
MiRNAs expression counts were obtained with samtools (z1.2)
Differential expression analysis was performed using the edgeR (Bioconductor version 2.13). Counts were normalized and differential expression was inferred using the negative binomial distribution and a shrinkage estimator for the distribution variance of the counts.
Assembly: mirBase: r21 mature RNA
Supplementary files format and content: raw count of reads mapping to different miRNAs for each sample

molecule: total RNA

organism: Mus musculus

source_name: kidney

extract_protocol:
Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure Approximately 5 µg of total RNA were used to prepare small RNA librariesaccording to the protocol of TruSeq Small RNA Sample Prep Kits (Illumina, San Diego, CA, USA). And then the libraries were sequenced by Illumina Hiseq 2500 at the LC-BIO following the vendor’s recommended protocol. Raw reads were subjected to an in-house program, ACGT101-miR (LC Sciences, Houston, Texas, USA) to remove adapter dimers, junk, low complexity, common RNA families (rRNA, tRNA, snRNA, snoRNA) and repeats.
RNA libraries were prepared for sequencing using standard Illumina protocols；

tag: tissue

value: kidney

tag: strain

tag: treatment

value: The same volumes of 0.9percentsaline simultaneously.


title: C1C-blue

city: Guangzhou

first: Jiemei

last: Chen

state: Guangdong

address: Tianhe Road

department: Rehabilitation Medicine

institute: Sun Yat-Sen Unv

data_processing:
Image analysis and base calling were performed using Solexa pipeline v1.8 (Off-Line Base Caller software, v1.8). Sequence quality was examined using the FastQC software. The trimmed reads (trimmed 5’,3’-adaptor bases using cutadapt) were aligned to reference genome using Hisat2 software (v2.0.4). The transcript abundances for each sample was estimated with StringTie(v1.2.3), and the FPKM value for gene level were calculated with R package Ballgown (v2.6.0). The differentially expressed genes were filtered using R package Ballgown. The novel genes were predicted from assembled results by comparing to the reference annotation using StringTie and Ballgown.
Genome_build: Rnor6
Supplementary_files_format_and_content: Gene expression files include Gene information and FPKM values for each Sample.
Supplementary_files_format_and_content: Novel gene expression files include Novel gene information and FPKM values for each Sample .

organism: Hippocampus

source_name: Rattus norvegicus

extract_protocol:
Total RNA was extracted from the hippocampi of 3 sham rats and 3 tMCAO rats at days 14 after the surgical intervention using TRIzol reagent, in accordance with the manufacturer’s  instructions.
RNA libraries were prepared for sequencing using standard lllumina protocols

treatment_protocol: tMCAO model protocol: After anesthetizing the rats, the left middle cerebral artery was occluded using a monofilament with a silica gel head end for 90 min then subsequently reperfused. Sham rats underwent the same protocol without occlusion of the middle cerebral artery.

tag: rat model

value: tMCAO

value: Sprague-Dawley rat

tag: group


title: C1C-red


title: Liver rep3 in EODR mice sacrified at ZT0

city: Changsha

first: Guolin

state: Hunan

address: 177 Lushan Road

department: College of Life Sciences

institute: Hunan Normal University

description: every-other-day refeeding (EODR)

data_processing:
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to GRCm38 whole genome using bowtie v0.12.2 with parameters -q -p 4 -e 100 -y -a -m 10 --best --strata
Reads Per Kilobase of exon per Megabase of library size (FPKM) were calculated using a protocol from Chepelev et al., Nucleic Acids Research, 2009. In short, exons from all isoforms of a gene were merged to create one meta-transcript. The number of reads falling in the exons of this meta-transcript were counted and normalized by the size of the meta-transcript and by the size of the library.
Data filtering steps.Prior to assembly, the low quality reads(1,reads containing sequencing adaptors; 2,reads containing sequencing primer;3, nucleotide with q quality score lower than 20 )were removed .software CutAdapter,parameters -o 5 -p 100
Read alignment software,software HISAT ,version 2.0 ,parameters -l fr-firststrand -mi 20 -mx 500000 -p 2 -b dta -q phred33-quals -x 9
perform expression level for mRNAs by calculating FPKM.stimate the expression levels of all transcripts.software StringTie ,version 1.3.0,parameters -b dta -q phred33-quals.
Genome_build: GRCm38
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample

source_name: Liver

growth_protocol: Six-week-old male C57BL/6N mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. Mice were housed in a temperature (25℃)-and light-controlled vivarium with free access to water and standard rodent chow food. All experiments were started with 8-week-old mice.

extract_protocol:
Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. Total RNA quantity and purity were analyzed by Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0.
RNA libraries were prepared for sequencing using standard Illumina protocols

treatment_protocol: Mice were randomly grouped to ad libitum (AL) group, acute fasting (AF) group, refeeding after acute fasting (AR) group, every-other-day fasting (EODF) group and every-other-day refeeding (EODR) group. All mice were single housed for 2 weeks prior to study initiation to allow for acclimation to the animal facility. The AL group mice were allowed unrestricted access to food. The mice of AF groups were allowed free access to food except the last day acute fasting for 4 h, 8 h, 12 h, 16 h, 20 h and 24 h, respectively, before sacrificed. The mice of AR groups were allowed free access to food except the last two days followed an acute fasting for 24 h, and then refeeding for 4 h, 8 h, 12 h, 16 h, 20 h and 24 h, respectively, before sacrificed. The mice of EODF and EODR groups were fed with 15 cycles of alternating 24 h periods of free access to food followed by 24-h fasting, then EODF mice were sacrificed after fasting for 4 h, 8 h, 12 h, 16 h, 20 h and 24 h, respectively, while EODR mice were sacrificed after refeeding for 4 h, 8 h, 12 h, 16 h, 20 h and 24 h, respectively. Considering the nocturnal eating characteristics of mice, all fasting and refeeding switches were started at Zeitgeber time 12.

value: Mice refed for 12h after 24h fasted before sacrificed


title: C1C-black


title: Con2


title: NSC H3K27ac cutrun

city: Cincinnati

first: Richard

last: Lu

state: OH

address: 3333 BURNET AVENUE

department: Brain Tumor Center

country: USA

institute: Cincinnati Children's Hospital Medical Center

library_source: genomic

data_processing:
Library strategy: CUT&RUN
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence. RNAseq and ChIPseq reads were aligned to mm10 using tophat2 and botwie2, respectively.
Fragments Per Kilobase of CDS per Megabase of library size (RPKM) were calculated for RNAseq.
ChIP-seq peak calling was performed using model-based analysis of ChIP-seq (MACS, version 1.4.2).
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include raw count values for each Sample.
Supplementary_files_format_and_content: bigwig files for ChIPseq

molecule: genomic DNA

source_name: Neural progenitor cells

growth_protocol: Mouse Neural Stem Cells (mNSC) were maintained in Neural Stem Cell (NSC) Basal Medium with NSC proliferation supplements, 10 ng/ml EGF, 20 ng/ml basic-FGF and 2 μg/ml Heparin (Stem Cell Technologies, Vancouver, Canada).

extract_protocol:
RNA from the normal brain, tumor tissues, and YAP-NSC cells were extracted using TRIzol (Life Technologies), followed by purification using a RNeasy Mini Kit (Qiagen). Illumina TruSeq RNA Sample Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries. Dissociated tissues were fixed for 15 min at room temperature with 1% formaldehyde-containing medium. Nuclei were isolated and sonicated in sonication buffer (10 mM Tris-HCl pH 8.0, 1 mM EDTA, 0.5 mM EGTA and protease inhibitor cocktail). Sonicated chromatin (~300 μg) was used for immunoprecipitation by incubation with appropriate antibodies (4 μg) overnight at 4℃.
RNA-seq libraries were prepared using Illumina RNA-Seq Preparation Kit. ChIP-seq libraries were prepared using NEBNext ChIP-seq Library Prep Master Mix Set for Illumina (NEB catalogue number E6240L)

value: YAP expressing ES-derived neural progenitor cells

tag: antibody

value: H3K27ac (Active motif, 39135)

value: overexpressing YAP


title: NSC YAP Cutrun

value: YAP1 (Cell signaling, 4912)


title: fwa_RNAseq_rep-2

city: Los Angeles

first: Zhenhui

last: Zhong

address: 610 Charles E Young Dr East

department: Department of Molecular, Cell and Developmental Biology

institute: University of California, Los Angeles

description: RNA-seq data of fwa replicate 2

data_processing:
All libraries were sequenced at a length of 50 bps with HiSeq 2500 or NovaSeq 6000 platforms following manufacturer’s instructions (Illumina). Raw reads were aligned to the Arabidopsis reference genome (TAIR10) with Bowtie2 (v2.1.0)43, allowing only uniquely mapped reads with perfect matches. Duplicated reads were removed with Samtools (v1.9)44. Peaks were called using MACS2 (v2.1.1)45.
BS-seq reads were mapped to TAIR10 reference genome by bsmap (v2.90) with allowing 2 mismatches and 1 best hit (-v 2 -w 1)46. Reads with three or more consecutively methylated CHH sites were considered as non-converted reads and removed from the analyses. DNA methylation levels were calculated by #C/ (#C + #T). Differential Methylated Regions (DMRs) were called by methdiff function with every 100bp bin for where the difference in CG, CHG, and CHH methylation are at least 0.4, 0.2, and 0.1, respectively.
Genome_build: tair10
Supplementary_files_format_and_content: bed, bigwig, wig
ATAC-seq analysis: ATAC-seq reads adaptors were removed with trim_galore. The reads were then mapped to Arabidopsis thaliana reference genome TAIR10 using Bowtie2 (-X 2000 -m 1)62. Reads of chloroplast and mitochondrial DNA were filtered and duplicate reads were removed using Samtools 63. ATAC-Seq open chromatin peaks of each replicate were called using MACS2 with parameters -p 0.01 --nomodel --shift -100 --extsize 200. Consensus set of chromatin peaks of each samples were merged by bedtools (v2.26.0) intersect with allowing 10 base pairs distance64. edgeR was used to define significantly changed peaks [Fold Change, (FC) > 2 and False Discovery Rate, (FDR) < 0.05]65. Chromosomal distributions of ATAC-seq were calculated by dividing Arabidopsis chromosomes into 100 Kb sized bins and count reads at each bin with bedtools. ATAC-seq peak distribution was annotated with ChIPseeker66. Arabidopsis protein coding genes were ranked and divided into 10 quantiles according to gene coding region length, and coding region of each gene was divided in proportion into 10 quantiles. Transcriptional factor footprints were analyzed by TOBIAS67 with 572 plant TF motifs downloaded from Jasper (http://jaspar.genereg.net/).
RNA-seq analysis: Cleaned short reads were aligned to reference genome tair10 by Bowtie2 (v2.1.0), and expression abundance was calculated by RSEM with default parameters68. Heatmaps were visualized with the R package pheatmap. To reduce false positive of differential expression, transcripts with less than 5 reads of all replicates in total were regarded as lowly expressed genes and have been removed in subsequent analysis. Differential expression analysis was conducted using edgeR. A threshold of p value < 0.05 and Fold Change > 2 were used to decide whether significant expression difference exists between samples.
ChIP-seq analysis: ChIP-seq fastq reads were aligned to the TAIR10 reference genome with Bowtie (v1.1.2)70, allowing only uniquely mapping reads with 0 mismatches. Duplicated reads were removed by Samtools. ChIP-seq peaks were called by MACS2 (v2.1.1) and annotated with ChIPseeker71. Differential peaks were called by bdgdiff function in MACS2. ChIP-seq data metaplots were plotted by deeptools (v2.5.1)72. For Pol II 5’ occupancy analysis, Pol II occupancy was calculated based normalized reads count (RPKM) on a TSS +/- 200 bp region and a TSS +500 bp to TTS gene body region by bedtools.
Whole genome bisulfite sequencing (BS-seq) analysis: Trim_galore (http://www.bioinformatics.babraham.ac.uk/projects/trim_galore/) was used to trim adapters after filtering low quality reads. BS-seq reads were aligned to TAIR10 reference genome by Bismark (v0.18.2)73 with default settings. Reads with three or more consecutive CHH sites were considered as unconverted reads and filtered. DNA methylation levels were defined as #C/ (#C + #T). DMRs (Differentially Methylated Regions) were called by DMRcaller with p < 0.01 for where the differences in CG, CHG, and CHH methylation were at least 0.4, 0.2, and 0.1, respectively.
TSS-seq analysis: TSS-seq data were analyzed following previously published pipeline. TSS-seq reads were trimmed with Trim_galore and 5’ end UMI barcodes were trimmed using UMI-Tools. The reads were then aligned to TAIR10 genome assembly using STAR (v2.7.0e)76. Mapping files were filtered with MAPQ<10 and deduplicated using SAMtools (v1.9) before converted to stranded Bedgraph files using bedtools (v2.26.0). TSS peaks were identified with CAGEfightR (v0.99.0) and differential TSS peaks [Fold Change, (FC) >2 and p value<0.05] were called with DESeq2 (v1.28.1).
BS-PCR analysis: BS-PCR data were analyzed with a previously published pipeline. BS-PCR data were trimmed with primer sequences and mapped to TAIR10 reference genome with bsmap (v2.90) allowing 2 mismatches and 1 best hit (-v 2 -w 1).

organism: Arabidopsis thaliana

source_name: Arabidopsis floral tissue

growth_protocol: Arabidopsis plants of the Columbia-0 (Col-0) ecotype were used in this study. All plants were grown under green house condition (16 h light/8 h dark, 22C). Floral tissues were collected from 5-6 week plants

extract_protocol:
ChIP seq: Libraries were prepared with Ovation Ultra Low System V2 kits following the manufacturer’s instructions. BS seeq: 300 ng of DNA was sheared to 200bp with a Covaris S2 (Covaris). Libraries were prepared with the Epitect Bisulfite Conversion kit (QIAGEN) and the Ovation Ultralow Methyl-seq kit (NuGEN) following the manufacturer’s instructions. RNA seq: 1 ug of total RNA was used for library preparation with TruSeq Stranded mRNA kit (Illumina). Libraries were sequenced on HiSeq 2500 or NovaSeq 6000 (Illumina).
All Arabidopsis plants used in this study are in Columbia (Col-0) ecotype and were grown at 22°C in LD (16 hours light, 8 hours dark) conditions.
ATAC-seq: The nuclei collection process from inflorescence and meristem tissues is as described previously. Freshly isolated nuclei were used for ATAC-seq as described elsewhere. Inflorescence tissues were collected for extraction of nuclei as follows. About 5 g of inflorescence tissue was collected and immediately transferred into ice-cold grinding buffer (300 mM sucrose, 20 mM Tris pH 8, 5 mM MgCl2, 5 mM KCl, 0.2% Triton X-100, 5 mM β-mercaptoethanol, and 35% glycerol). The samples were ground with Omni International General Laboratory Homogenizer at 4°C and then filtered through a two-layer Miracloth and a 40-µm nylon mesh Cell Strainer (Fisher). Samples were spin filtered for 10 min at 3,000 g, the supernatant was discarded, and the pellet was resuspended with 25 ml of grinding buffer using a Dounce homogenizer. The wash step was performed twice in total, and nuclei were resuspended in 0.5 ml of freezing buffer (50 mM Tris pH 8, 5 mM MgCl2, 20% glycerol, and 5 mM β-mercaptoethanol). Nuclei were subjected to a transposition reaction with Tn5 (Illumina). For the transposition reaction, 25 µl of 2x DMF (66 mM Tris-acetate pH 7.8, 132 mM K-Acetate, 20 mM Mg-Acetate, and 32% DMF) was mixed with 2.5 µl Tn5 and 22.5 µl nuclei suspension at 37°C for 30 min. Transposed DNA fragments were purified with ChIP DNA Clean & Concentrator Kit (Zymo). Libraries were prepared with Phusion High-Fidelity DNA Polymerase (NEB) in a system containing 12.5 µl 2x Phusion, 1.25 µl 10 mM Ad1 primer, 1.25 µl 10 mM Ad2 primer, 4 µl ddH2O, and 6 µl purified transposed DNA fragments. The ATAC-seq libraries were sequenced on HiSeq 2500 platform (Illumina).
Plants total RNAs were extracted with TRIzol and Direct-zol RNA Miniprep kit (Zymo, R2050). Sequencing libraries were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) following the manufacturer instructions.
RNA-seq library preparation: Plants total RNAs were extracted with TRIzol and Direct-zol RNA Miniprep kit (Zymo, R2050). Sequencing libraries were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) following the manufacturer instructions.
ChIP-seq library preparation: 10 grams of inflorescence and meristem tissues were used for Flag, HA, Pol II, H3, and H3K36me2/3 ChIP-seq. ChIP were performed as described previously60 with anti-FLAG M2 (Sigma), anti-HA (Roche), anti-Pol II (Ab26721, Abcam), anti-H3 (Ab1791, Abcam), anti-H3K36me2 (Ab9049, Abcam), and anti-H3K36me3 (Ab9050, Abcam) antibodies. Anti-NRPB1 antibody was raised in rabbits and further affinity-purified by ABClonal (China) using the peptide HEGDKKDKTGKKDASKDDK. To evaluate Pol II occupancy at both 5' end and 3' end of gene body, we selected anti-RNA Pol II CTD repeat YSPTSPS antibody which can capture Pol II signal at both regions (Ser5P and Ser2P). Libraries were prepared with NuGen Ovation Ultra Low System V2 kit following the manufacturer instructions.
Whole genome bisulfite sequencing (BS-seq) library preparation: Leaf tissue was used as starting material for BS-seq libraries preparation. Genomic DNA was extracted and converted with bisulfite treatment with EpiTect Bisulfite Kit (Qiagen) following the manufacturer instructions.
TSS-seq library preparation: TSS-seq was performed on 14 day old seedlings grown on MS plate following previous protocol. Heat stress was conducted by treating plants with 2 hours of 37°C. Five micrograms of total RNA were treated with DNase and CIP (NEB) to remove DNA and all non-capped RNA. Then 5’ caps of capped RNA were removed with Cap-Clip (CellScript) and single-stranded rP5_RND adapter were ligated to 5’-ends with T4 RNA ligase 1 (NEB). Ligated RNAs were enriched and captured by oligo(dT) Dynabeads (Thermo Fisher Scientific). Enriched samples were fragmented for 5 mins at 80°C and first-strand cDNA was generated with SuperScript III (Invitrogen) and random primers. Second-strand cDNA was synthesized with Phusion High-Fidelity DNA Polymerase (NEB) and the BioNotI-P5-PET oligo, and captured by Dynabeads for end repairing with End Repair Enzyme Mix (NEB) and ligation with barcoded Illumina compatible adapter using T4 DNA Ligase (NEB). TSS-seq sequencing libraries were amplified and size selected for single-end sequencing with NovaSeq 6000 platform (Illumina).
BS-PCR and McrBC assay for FWA tandem repeat: For BS-PCR of FWA tandem repeat, genomic DNA was extracted from leaf tissue of ASF1B-ZF T2 with CTAB-based method and converted using the EZ DNA Methylation-Lighting kit (ZYMO research). Methylation level of FWA promoter region and several control regions have been amplified with primers described previously46. HiSeq 2500 (Illumina) sequencing libraries were made from purified PCR products using a Kapa Hyper DNA Library Prep kit. For McrBC of FWA tandem repeat, genomic DNA was extracted from leaf tissue with CTAB-based method, and treated with PureLink RNase (Invitrogen) and then with McrBC (NEB) for 4 hours at 37°C. FWA tandem repeat region was quantified by qPCR.
ATAC-seq; RNA-seq; ChIP-seq; WGBS; TSS-seq; BS-PCR;

value: floral tissue

tag: developmental stage

value: 5-6 weeks


title: Liver rep1 in AL mice sacrified at ZT0

description: ad libitum (AL)

value: Mice fed ad libitum


title: fwa_RNAseq_rep-1

description: RNA-seq data of fwa replicate 1


title: Liver rep4 in AR mice sacrified at ZT0

description: refeeding after acute fasting (AR)


title: High-defective virus population 2, interferon depleted, replicate 3

city: San Diego

first: Alistair

middle: Brian

last: Russell

state: CA

address: 9500 Gilman Dr,

department: Biological Sciences

institute: University of California, San Diego

description: amplicon sequencing

library_source: other

data_processing:
Data were processed with a custom pipeline described at https://github.com/Russell-laboratory/NS1_interferon_variation
genome build/assembly: Influenza A virus (A/WSN/1933(H1N1))
Sample_supplementary_files_format_and_content: Deletion junctions, total fragments, and nucleotide/position data in tabulated files

molecule: other

organism: Influenza A virus (A/WSN/1933(H1N1))

extract_protocol: vRNA was sequenced

tag: cell type

tag: agent

value: High-defective virus population 2

value: interferon-depleted


title: ZF-ASF1B_RNAseq_rep-3

description: RNA-seq data of ZF-ASF1B replicate 3


title: ZF-ASF1B_RNAseq_rep-2

description: RNA-seq data of ZF-ASF1B replicate 2


title: High-defective virus population 2, interferon depleted, replicate 1

description: amplicon sequencing


title: Liver rep3 in AR mice sacrified at ZT0

description: refeeding after acute fasting (AR)


title: ZF-ASF1B_RNAseq_rep-1

description: RNA-seq data of ZF-ASF1B replicate 1


title: YM YAP-2 ChIP

data_processing:
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence. RNAseq and ChIPseq reads were aligned to mm10 using tophat2 and botwie2, respectively.
Fragments Per Kilobase of CDS per Megabase of library size (RPKM) were calculated for RNAseq.
ChIP-seq peak calling was performed using model-based analysis of ChIP-seq (MACS, version 1.4.2).
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include raw count values for each Sample.
Supplementary_files_format_and_content: bigwig files for ChIPseq

source_name: brain tumor tissue

value: brain tumor

value: YAP (Cell Signaling Technology, 4912)

value: IUE with YAP-M


title: YM YAP-1 ChIP


title: High-defective virus population 2, interferon enriched, replicate 3

description: amplicon sequencing

value: interferon-enriched


title: fas2_RNAseq_rep-3

description: RNA-seq data of fas2 replicate 3


title: NSC-YAP2

value: overepxressing YAP


title: fwa_RNAseq_rep-3

description: RNA-seq data of fwa replicate 3


title: Liver rep2 in EODR mice sacrified at ZT0

description: every-other-day refeeding (EODR)


title: fas2_RNAseq_rep-2

description: RNA-seq data of fas2 replicate 2


title: Liver rep1 in EODR mice sacrified at ZT0

description: every-other-day refeeding (EODR)


title: Liver rep2 in AR mice sacrified at ZT0

description: refeeding after acute fasting (AR)


title: High-defective virus population 2, interferon enriched, replicate 1

description: amplicon sequencing


title: fas2_RNAseq_rep-1

description: RNA-seq data of fas2 replicate 1


title: High-defective virus population 2, interferon depleted, replicate 2

description: amplicon sequencing


title: Liver rep5 in AR mice sacrified at ZT0

description: refeeding after acute fasting (AR)


title: Liver rep1 in AR mice sacrified at ZT0

description: refeeding after acute fasting (AR)


title: NSC-YAP1


title: YM H3K27ac ChIP


title: High-defective virus population 1, interferon depleted, replicate 3

description: amplicon sequencing

value: High-defective virus population 1


title: Liver rep5 in EODF mice sacrified at ZT0

description: every-other-day fasting (EODF)

value: Mice fasted for 12h before sacrificed


title: fas1_RNAseq_rep-3

description: RNA-seq data of fas1 replicate 3


title: SVZ3

value: wild-type


title: High-defective virus population 1, interferon depleted, replicate 2

description: amplicon sequencing


title: fas1_RNAseq_rep-2

description: RNA-seq data of fas1 replicate 2


title: Liver rep4 in EODF mice sacrified at ZT0

description: every-other-day fasting (EODF)


title: SVZ2


title: Liver rep3 in EODF mice sacrified at ZT0

description: every-other-day fasting (EODF)


title: High-defective virus population 1, interferon depleted, replicate 1

description: amplicon sequencing


title: SVZ1


title: fas1_RNAseq_rep-1

description: RNA-seq data of fas1 replicate 1


title: YMdIDR3

value: YAP-MΔIDR expressing ES-derived neural progenitor cells

value: overexpressing YAP-MΔIDR


title: High-defective virus population 2, interferon enriched, replicate 2

description: amplicon sequencing


title: High-defective virus population 1, interferon enriched, replicate 3

description: amplicon sequencing


title: Liver rep1 in EODF mice sacrified at ZT0

description: every-other-day fasting (EODF)


title: hira_RNAseq_rep-3

description: RNA-seq data of hira replicate 3


title: Liver rep2 in EODF mice sacrified at ZT0

description: every-other-day fasting (EODF)


title: High-defective virus population 1, interferon enriched, replicate 2

description: amplicon sequencing


title: Liver rep5 in AF mice sacrified at ZT0

description: acute fasting (AF)


title: hira_RNAseq_rep-2

description: RNA-seq data of hira replicate 2


title: YMdIDR2


title: High-defective virus population 1, interferon enriched, replicate 1

description: amplicon sequencing


title: Liver rep4 in AF mice sacrified at ZT0

description: acute fasting (AF)


title: asf1a1b_RNAseq_rep-3

description: RNA-seq data of asf1a1b replicate 3


title: YAP-M4

value: In utero electroporation with YAP-M


title: transcription start sites from SNHG1-T promoter

city: New Haven

first: Carson

middle: C

last: Thoreen

state: CT

address: 333 Cedar St. SHM B 145

department: Cellular and Molecular Physiology

institute: Yale School of Medicine

data_processing:
Basecalls performed using CASAVA 1.8.
In one step, read by read, sequenced reads were trimmed for adaptor sequences and counted using a custom script. For all RNA molecules the 3’ adapter sequence GCCGCAGCCGCCGCCATCGTCGACGC was removed. Reads were then aligned to expected promoter sequences (chr11:62,855,864-62,855,926 for KARS and chr11:62,855,865-62,856,083 for SNHG1 in hg38) using bowtie2 and coverage was calculated using samtools.
Supplementary files format and content: Processed data files are text files showing the number of reads aligning to each position of the respective promoters.

organism: Homo sapiens

growth_protocol: HEK-293T cells were grown in high-glucose DMEM supplemented with 10% (vol/vol) heat-inactivated FBS and penicillin/streptomycin. Cells were seeded on 10 cm plates at indicated numbers per plate the day before experiment.

extract_protocol:
Total RNA was extracted directly with Trizol.
The Renilla luciferase RNA was reverse transcribed with a gene-specific primer, followed by alkaline RNA hydrolysis. The 3’ end of the cDNA was then ligated to a double stranded DNA linker with a 3’ NNNNNN or GNNNNN (mixed in 1:4 ratio). A 3’ adapter sequence was added by PCR, followed by a second PCR that incorporates Illumina sequences with dual index. Sequencing libraries from plasmid were prepared by adding adapter sequences in a first PCR step, followed by incorporation of illumina sequences in a second PCR reaction.

treatment_protocol: Cells were transiently transfected with the indicated plasmids containing the KARS or SNHG1 variant promoters.

tag: cell line

tag: transfection

value: plasmid containing SNHG1-T promoter

tag: rna fraction

value: all


title: Liver rep3 in AF mice sacrified at ZT0

description: acute fasting (AF)


title: hira_RNAseq_rep-1

description: RNA-seq data of hira replicate 1

value: floral tissue

value: 5-6 weeks


title: YAP-M3


title: transcription start sites from SNHG1-C promoter

value: plasmid containing SNHG1-C promoter


title: asf1a1b_RNAseq_rep-2

description: RNA-seq data of asf1a1b replicate 2


title: YAP-M1


title: Liver rep2 in AF mice sacrified at ZT0

description: acute fasting (AF)


title: Liver rep1 in AF mice sacrified at ZT0

description: acute fasting (AF)


title: asf1a1b_RNAseq_rep-1

description: RNA-seq data of asf1a1b replicate 1


title: transcription start sites from KARS-T promoter

value: plasmid containing KARS-T promoter


title: RASY3wk4

city: Bethesda

first: Howard

last: Yang

state: MD

address: 37 CONVENT DR, Room 5120

department: CCR

institute: NCI

data_processing:
RNA sequencing was peformed by Illumina HiSeq 2500
Reads of the samples were aligned against Mouse genome mm10 using TopHat2.1.1
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files including raw counts and  RPKM values for each Sample

extract_protocol:
Cells in culture vessels were lysed with RLT lysis buffer containing 1% beta mercaptoethanol after 24 hours and RNA was harvased using QIAcube as per Rneasy kit from Qiagen.
RNA libraries were prepared for sequencing using standard Illumina protocols

value: BALB/c

value: Primary mouse keratinocytes

value: RAS with Y27632, three weeks


title: Liver rep5 in AL mice sacrified at ZT20

description: ad libitum (AL)

value: Mice fed ad libitum


title: transcription start sites from KARS-C promoter

value: plasmid containing KARS-C promoter


title: Col_RNAseq_rep-4

description: RNA-seq data of Col-0 replicate 4


title: Liver rep4 in AL mice sacrified at ZT20

description: ad libitum (AL)


title: RASY3wk3


title: YMdIDR1


title: Col_RNAseq_rep-3

description: RNA-seq data of Col-0 replicate 3


title: Liver rep3 in AL mice sacrified at ZT20

description: ad libitum (AL)


title: transcription start sites from KARS-A promoter

value: plasmid containing KARS-A promoter


title: RASY3wk2


title: RASY3wk1


title: Liver rep2 in AL mice sacrified at ZT20

description: ad libitum (AL)


title: YAP-M2

value: brain tumor


title: RAS3wk4

value: RAS, three weeks


title: Liver rep1 in AL mice sacrified at ZT20

description: ad libitum (AL)


title: reporter RNA from the subpolysomal fraction of HeLa cells treated with Torin1 for 2 hours, rep 2

data_processing:
Basecalls performed using CASAVA 1.8.
In one step, read by read, sequenced reads were trimmed for adaptor sequences and counted using a custom script. For all RNA molecules the 3’ adapter sequence GCCGCAGCCGCCGCCATCGTCGACGC was removed, whereas for the DNA molecules (from plasmid) the same 3’ adapter as well as the 5’ adapter sequence AGAGCTGGTTTAGTGAACCGTC were removed. For the RNA-derived libraries, a read was only counted if the trimmed sequence was 1) 7-mers not starting with a G or N, or 2) 8-mers starting with a G. This first G would be removed before counting. For the plasmid-derived library all trimmed sequences that were 7-mers were counted. In this same first step all reads corresponding to the spike-in RNA (containing sequence CTCTTCCCATGGCCGCAGCCGCCGCCATCGTCGACGCGCGCT) were counted.
Using DESeq2, read counts were normalized using spike-in counts first. Then RNA stability and RNA translation rate and the fold change in each (Torin1/DMSO) were calculated per 5’ end 7-mer sequence. RNA stability was estimated by calculating the ratio of actinomycinD-treated over untreated normalized read counts, per condition (Torin1 or control-treated). RNA translation rates were estimated as the ratio of polysomal over subpolysomal normalized read counts per condition.
Genome_build: NOT PROVIDED
Supplementary_files_format_and_content: counts

source_name: HeLa cells

growth_protocol: HeLa cells were grown in high-glucose DMEM supplemented with 10% (vol/vol) heat-inactivated FBS and penicillin/streptomycin. Cells were seeded on 15 cm plates at indicated numbers per plate the day before experiment.

extract_protocol:
Either total RNA was extracted directly with Trizol containing a capped RNA spike-in molecule, or cells were lysed on plate in 20 mM Tris pH 7.4; 150 mM NaCl; 5 mM MgCl2; 1% Triton X-100; 1 mM DTT; 100 ug/ml cycloheximide. Clarified lysates were then separated into a subpolysomal and polysomal fraction on a 5-50% sucrose gradient, and an equal amount of the capped spike-in RNA described above was added to each fraction. Total RNA was retrieved from both fractions by proteinase K treatment followed by acid phenol extraction.
The Renilla luciferase RNA was reverse transcribed with a gene-specific primer, followed by alkaline RNA hydrolysis. The 3’ end of the cDNA was then ligated to a double stranded DNA linker with a 3’ NNNNNN or GNNNNN (mixed in 1:4 ratio). A 3’ adapter sequence was added by PCR, followed by a second PCR that incorporates Illumina  sequences with dual index. Sequencing libraries from plasmid were prepared by adding adapter sequences in a first PCR step, followed by incorporation of Illumina  sequences in a second PCR reaction.

treatment_protocol: Cells were treated with 250 nM Torin1 or an equal volume of DMSO (0.025% final concentration) for 2 hours. Then cells were either lysed or 2 ug/ml actinomycinD was added followed by another 2 hour incubation.

value: HeLa

value: subpolysomal fraction


title: reporter RNA from the subpolysomal fraction of HeLa cells treated with Torin1 for 2 hours, rep 1


title: reporter RNA from the polysomal fraction of HeLa cells treated with Torin1 for 2 hours, rep 2

value: polysomal fraction


title: RAS3wk3


title: reporter RNA from the polysomal fraction of HeLa cells treated with Torin1 for 2 hours, rep 1


title: Liver rep4 in EODR mice sacrified at ZT20

description: every-other-day refeeding (EODR)

value: Mice refed for 8h after 24h fasted before sacrificed


title: transcription start sites from SNHG1-A promoter

value: plasmid containing SNHG1-A promoter

value: all


title: Col_RNAseq_rep-2

description: RNA-seq data of Col-0 replicate 2

value: floral tissue

value: 5-6 weeks


title: fas2_WGBS_rep-2

description: WGBS data of fas2 replicate 2

source_name: Arabidopsis leaf tissue

value: leaf tissue


title: Liver rep5 in EODR mice sacrified at ZT20

description: every-other-day refeeding (EODR)


title: RAS3wk2

value: BALB/c


title: reporter RNA from the subpolysomal fraction of HeLa cells treated with DMSO for 2 hours, rep 2

value: DMSO


title: fas1_WGBS_rep-2

description: WGBS data of fas1 replicate 2


title: Liver rep3 in EODR mice sacrified at ZT20

description: every-other-day refeeding (EODR)


title: RAS3wk1

value: Primary mouse keratinocytes


title: Liver rep2 in EODR mice sacrified at ZT20

description: every-other-day refeeding (EODR)


title: RASY1wk4

value: RAS with Y27632, one week


title: fas1_WGBS_rep-1

description: WGBS data of fas1 replicate 1


title: reporter RNA from the polysomal fraction of HeLa cells treated with DMSO for 2 hours, rep 2


title: hira_WGBS_rep-2

description: WGBS data of hira replicate 2


title: Col_RNAseq_rep-1

description: RNA-seq data of Col-0 replicate 1


title: reporter RNA from the subpolysomal fraction of HeLa cells treated with DMSO for 2 hours, rep 1


title: hira_WGBS_rep-1

description: WGBS data of hira replicate 1


title: fas2_WGBS_rep-1

description: WGBS data of fas2 replicate 1


title: Liver rep1 in EODR mice sacrified at ZT20

description: every-other-day refeeding (EODR)


title: reporter RNA from the polysomal fraction of HeLa cells treated with DMSO for 2 hours, rep 1


title: RASY1wk3


title: RASY1wk2


title: Liver rep5 in AR mice sacrified at ZT20

description: refeeding after acute fasting (AR)


title: Liver rep4 in AR mice sacrified at ZT20

description: refeeding after acute fasting (AR)


title: reporter RNA from HeLa cells treated with Torin1 for 2 hours, then actinomycin D for 2 hours, rep 1

value: Torin1 and actinomycin D


title: reporter RNA from HeLa cells treated with Torin1 for 2 hours, then actinomycin D for 2 hours, rep 2


title: RAS1wk4

value: RAS, one week


title: Liver rep2 in AR mice sacrified at ZT20

description: refeeding after acute fasting (AR)


title: asf1a1b_WGBS_rep-1

description: WGBS data of asf1a1b replicate 1


title: Liver rep3 in AR mice sacrified at ZT20

description: refeeding after acute fasting (AR)


title: reporter RNA from HeLa cells treated with Torin1 for 2 hours, rep 2


title: RAS1wk3


title: asf1a1b_WGBS_rep-2

description: WGBS data of asf1a1b replicate 2


title: RASY1wk1


title: Liver rep1 in AR mice sacrified at ZT20

description: refeeding after acute fasting (AR)


title: fwa_ATACseq_rep-2

description: ATAC-seq data of fwa replicate 2


title: Col_WGBS_rep-1

description: WGBS data of Col-0 replicate 1


title: Col_WGBS_rep-2

description: WGBS data of Col-0 replicate 2


title: reporter RNA from HeLa cells treated with Torin1 for 2 hours, rep 1


title: RAS1wk2


title: reporter RNA from HeLa cells treated with DMSO for 2 hours, then actinomycin D for 2 hours, rep 2

value: DMSO and actinomycin D


title: Liver rep5 in EODF mice sacrified at ZT20

description: every-other-day fasting (EODF)

value: Mice fasted for 8h before sacrificed


title: RAS1wk1


title: reporter RNA from HeLa cells treated with DMSO for 2 hours, then actinomycin D for 2 hours, rep 1

value: HeLa


title: fwa_ATACseq_rep-1

description: ATAC-seq data of fwa replicate 1


title: ZF-ASF1B_ATACseq_rep-2

description: ATAC-seq data of ZF-ASF1B replicate 2


title: Control1wk4

value: untreated one week


title: reporter RNA from HeLa cells treated with DMSO for 2 hours, rep 2


title: Liver rep4 in EODF mice sacrified at ZT20

description: every-other-day fasting (EODF)


title: ZF-ASF1B_ATACseq_rep-1

description: ATAC-seq data of ZF-ASF1B replicate 1


title: Control1wk3


title: reporter RNA from HeLa cells treated with DMSO for 2 hours, rep 1


title: Liver rep2 in EODF mice sacrified at ZT20

description: every-other-day fasting (EODF)


title: Control1wk2


title: fas2_ATACseq_rep-2

description: ATAC-seq data of fas2 replicate 2


title: fas2_ATACseq_rep-1

description: ATAC-seq data of fas2 replicate 1


title: H5286Cin

city: New York

first: Osama

last: Al-Dalahmah

state: NY

address: 630 West, 168th Street

department: Pathology and Cell Biology

institute: Columbia University

data_processing:
Alignment was done using the CellRanger pipeline (10X Genomics) to GRCh38.p12 (refdata-cellrangerGRCh38–1.2.0 file provided by 10x Genomics).
Count matrices were generated from BAM files using default parameters of the DropEst pipeline.
Filtering low quality cells: we first used the combined quality calls from the CellRanger algorithm as well as the DropEst algorithm. This allowed us to retain more high quality nuclei than either algorithm alone. Data 562  QC was done using the scater package 48. Nuclei with percent exonic reads from all reads in the range of less 75% were included. Nuclei with percent mitochondrial reads aligning to mitochondria genes of more than 14% were excluded. Genes were filtered by keeping features with > 10 counts per row in at least in 31 cells. A temporary count slot was created by decontaminating the counts from ambient RNA by calling decontX() function in R . These counts were used for downstream clustering, but not differential gene expression analysis.
to correct for sequencing and technical batches, we utilized Liger and SeuratWrappers for Seurat. Briefly, Seurat objects containing raw reads were normalized in Seurat by calling NormalizeData() on the combined object. Variable features were then detected by calling FindVariableFeatures(). We then scaled the data by calling ScaleData(object, split.by = "Batch", do.center = FALSE). Next, we called RunOptimizeALS(object, k = 20, lambda = 5, split.by = "Batch") and RunQuantileAlignSNF(object, split.by = "Batch").
Normalization of counts was done using the SCTrasform function in Seurat, accounting for mitochondrial reads and batch
Genome_build: GRCh38
Supplementary_files_format_and_content: filtered_cell_counts.csv: raw unnormalized counts filtered by parameters listed aboves used for normalization and batch correction as presented in the methods and described above. Used for all data presented in the manuscript
Supplementary_files_format_and_content: metadata.csv: metadata file with relevant information on each cell (column name) in the filtered_cell_counts.csv file including condiiton, location, case numer, and batch

source_name: Human brain – Cingulate Cortex

extract_protocol:
The tissue was homogenized in a Dounce homogenizer with 12–15 strokes of the loose pestle and 12–15 strokes of the tight pestle on ice in a Triton X-100 based, sucrose containing buffer. The suspension from each sample was filtered through a BD Falcon tubes with a cell strainer caps (Becton Dickinson, cat. no. 352235), washed, re-filtered, washed, followed by a cleanup step using iodixanol gradient centrifugation. The nuclear pellet was then re-suspended in 1% BSA in 545 nuclease-free PBS (containing RNAse inhibitors) and titrated to 600-1200 nuclei/μl.
Nuclear suspensions were processed by the Chromium Controller (10x Genomics) using single Cell 3′ Reagent Kit v2 or v3 (Chromium Single Cell 3′ Library & Gel Bead Kit v2/v3, catalog number PN-1000075; Chromium Single Cell A Chip Kit, 48 runs, catalog number: 120236; 10x Genomics). Sequencing and alignment: Sequencing of the snRNAseq libraries was done on Illumina NOVAseq 6000 platformV4 150 bp paired end reads.

value: brain

tag: region

value: Cingulate Cortex


title: Liver rep1 in EODF mice sacrified at ZT20

description: every-other-day fasting (EODF)


title: C5700Cin


title: C5596Cin


title: fas1_ATACseq_rep-2

description: ATAC-seq data of fas1 replicate 2


title: 266T

city: Bogotá

first: Natalia

middle: Lizeth

last: Acosta Vega

state: Bogotá, D.C.

country: Colombia

institute: Instituto Nacional de Cancerología - Colombia

description: no processed data

data_processing:
Quality control using FASTQC
Aligned to human ribosomal RNA using STAR® v.2.5.2.
Unmapped reads to ribosomal RNA were used to map to human reference Homo_sapiens.GRCh38.78 (Ensembl).
Read counts and expression calculations were done using RSEM® v1.2.31.
Filtering for protein coding genes according to ENSEMBL.
Principal component analysis (PCA) excluded 2 outlier samples.
Genes with median counts of zero in all samples were filtered out.
Assembly: Homo_sapiens.GRCh38.78 (Ensembl)
Supplementary files format and content: Tab-delimited text file including raw counts for all samples.

source_name: Prostate tumor - FFPE

extract_protocol:
Total RNA was extracted using AllPrep DNA/RNA FFPE kit® (Qiagen, Hilden, Germany) following the manufacturer’s recommendations. RNA quantity and quality were determined with Nanodrop 2000 Spectrophotometer® (ThermoFisher Scientific, Wilmington, USA) and the Agilent RNA 6000 Nano kit® (Agilent Technologies, Santa Clara, CA), respectively. All samples were suitable for library preparation.
For library preparation, 1 g of total RNA and the TruSeq Stranded Total RNA Library Prep kit® with Ribo-Zero Human/Mouse/Rat (Illumina, Inc., San Diego, CA, USA) were used. Fragmentation step was omitted in most of the samples due to sample quality, while in five samples it was done according to recommendations from Illumina®. Validation of libraries was performed in the Agilent 2100 Bioanalyzer® system and then normalization to 10 nM was done with the Qubit dsDNA HS Assay kit® (ThermoFisher Scientific, Wilmington, USA), before cluster generation. Sequencing was performed in 12-pooled samples at 1 x 75 bp with single-end strategy, with no sequencing results in six tumor samples.

treatment_protocol: Samples were obtained from FFPE (Formalin Fixed Paraffin Embedded) tissues from radical prostatectomies (RP). Patients did not have previous treatments.

value: Prostate tumor - FFPE

value: Radical prostatectomy

tag: previous treatment

value: No

tag: age at_diagnosis

tag: bmi

tag: erg status

tag: time to_bcr_(days)

tag: gleason grade_group_at_rp

tag: clinical stage_(ct)


title: fas1_ATACseq_rep-1

description: ATAC-seq data of fas1 replicate 1

tag: tissue

value: floral tissue

value: 5-6 weeks

tag: strain


title: Liver rep4 in AF mice sacrified at ZT20

description: acute fasting (AF)

tag: treatment


title: Liver rep3 in AF mice sacrified at ZT20

description: acute fasting (AF)


title: Liver rep5 in AF mice sacrified at ZT20

description: acute fasting (AF)


title: Control1wk1

value: BALB/c


title: hira_ATACseq_rep-2

description: ATAC-seq data of hira replicate 2

tag: developmental stage


title: 245T

description: no processed data


title: H5608Cin


title: H5263Cin


title: Liver rep3 in EODF mice sacrified at ZT20

description: every-other-day fasting (EODF)


title: C5227Cin


title: 202T

description: no processed data

value: Yes


title: hira_ATACseq_rep-1

description: ATAC-seq data of hira replicate 1


title: asf1a1b_ATACseq_rep-2

description: ATAC-seq data of asf1a1b replicate 2


title: Liver rep1 in AF mice sacrified at ZT20

description: acute fasting (AF)


title: H5109Cin


title: 295T

value: low


title: 288T


title: H4161Cin


title: Liver rep5 in AL mice sacrified at ZT16

description: ad libitum (AL)

value: Mice fed ad libitum


title: 263T

description: no processed data


title: Liver rep4 in AL mice sacrified at ZT16

description: ad libitum (AL)


title: asf1a1b_ATACseq_rep-1

description: ATAC-seq data of asf1a1b replicate 1


title: RNA.kcl_7h_2

city: Haifa

first: Reut

last: Shalgi

address: 1 Efron st.

department: Faculty of Medicine

country: Israel

institute: Technion

description:
Rnaseq.rsem.genes.expected_count.txt
Rnaseq.rsem.genes.tpm.txt
dogs_per_sample.txt
read_in.estimation.txt
read_in_per_sample.txt

data_processing:
RNA-Seq analysis: reads were filtered for rRNAs using STAR. The remaining reads were mapped to mm10 version of mouse genome using STAR. Expression levels were quantified using RSEM to produce transcript and gene level quantification TPM (Transcript Per Million) values.
Riboseq analysis: reads were filtered for rRNAs, tRNAs and snRNAs using STAR. The remaining reads were mapped to coding sequences of the mm10 mouse genome annotation using Bowtie. Expression levels were quantified using RSEM to produce transcript and gene level quantification TPM (Transcript Per Million) values.
Genome_build: mm10
Supplementary_files_format_and_content: Tab-delimited text files with read count and TPM data for genes, produced by RSEM, as well as DoG genes, read-in genes and read-in estimations, produced by custom scripts

source_name: NIH3T3 cells

extract_protocol: Rna-Seq libraries were made using  NEB E7760 kit. Riboseq libraries were made as described in Sabath et al (https://doi.org/10.1073/pnas.2018138117).

tag: cell type

value: Mouse embryonic fibroblasts NIH3T3

tag: stress type

value: Osmotic stress (KCl, 7h)

tag: replicate

tag: molecule type

value: PolyA-selected mRNA


title: H5608Cd

source_name: Human brain – Caudate Nucleus

value: Caudate Nucleus


title: Col_ATACseq_rep-2

description: ATAC-seq data of Col-0 replicate 2


title: Col_ATACseq_rep-1

description: ATAC-seq data of Col-0 replicate 1


title: 282T

value: high


title: H5263Cd


title: RNA.kcl_7h_1


title: Liver rep2 in AL mice sacrified at ZT16

description: ad libitum (AL)


title: 285T


title: 270T


title: Liver rep2 in AF mice sacrified at ZT20

description: acute fasting (AF)

value: Mice fasted for 8h before sacrificed


title: 268T


title: Liver rep5 in EODR mice sacrified at ZT16

description: every-other-day refeeding (EODR)

value: Mice refed for 4h after 24h fasted before sacrificed


title: RNA.kcl_2h_1

value: Osmotic stress (KCl, 2h)


title: Liver rep1 in AL mice sacrified at ZT16

description: ad libitum (AL)


title: RNA.kcl_2h_2


title: 267T

value: Prostate tumor - FFPE


title: C4812Cd

value: brain


title: H5109Cd


title: Liver rep3 in AL mice sacrified at ZT16

description: ad libitum (AL)


title: Liver rep3 in EODR mice sacrified at ZT16

description: every-other-day refeeding (EODR)


title: RNA.hs_ctrl_1

value: Control for heat stress


title: 287T

value: Radical prostatectomy

value: No


title: C5227Cd


title: C5832Cd


title: H4161Cd


title: RNA.hs_8M_2

value: Heat stress (42°C, 8h)


title: 257T


title: RNA.hs_ctrl_2


title: H5608A

source_name: Human brain – Nucleus Accumbens

value: Nucleus Accumbens


title: H5263A


title: RNA.hs_8M_1


title: 256T


title: Liver rep4 in EODR mice sacrified at ZT16

description: every-other-day refeeding (EODR)


title: C5227A


title: RNA.hs_2S_2

value: Heat stress (44°C, 2h)


title: 255T

value: low

value: Yes


title: Liver rep3 in AR mice sacrified at ZT16

description: refeeding after acute fasting (AR)


title: Liver rep1 in EODR mice sacrified at ZT16

description: every-other-day refeeding (EODR)


title: Liver rep4 in AR mice sacrified at ZT16

description: refeeding after acute fasting (AR)


title: Liver rep5 in AR mice sacrified at ZT16

description: refeeding after acute fasting (AR)


title: Liver rep2 in EODR mice sacrified at ZT16

description: every-other-day refeeding (EODR)


title: 249T


title: H5109A


title: 239T


title: 264T


title: RNA.h2o2_RNA.kcl_ctrl_2

value: Mouse embryonic fibroblasts NIH3T3

value: Control for H2O2 and KCl


title: Liver rep2 in AR mice sacrified at ZT16

description: refeeding after acute fasting (AR)


title: 229T


title: H5575Cd

value: Caudate Nucleus


title: 223T

value: high


title: H4161A


title: Liver rep5 in EODF mice sacrified at ZT16

description: every-other-day fasting (EODF)

value: Mice fasted for 4h before sacrificed


title: 5596Cd


title: H5493Cd


title: RNA.h2o2_RNA.kcl_ctrl_1

value: PolyA-selected mRNA


title: Liver rep1 in AR mice sacrified at ZT16

description: refeeding after acute fasting (AR)

value: Mice refed for 4h after 24h fasted before sacrificed


title: 218T

value: Prostate tumor - FFPE


title: RNA.hs_2S_1


title: RNA.h2o2_7h_1

value: Oxidative stress (H2O2, 7h)


title: Liver rep4 in EODF mice sacrified at ZT16

description: every-other-day fasting (EODF)


title: 182T


title: Liver rep3 in EODF mice sacrified at ZT16

description: every-other-day fasting (EODF)


title: Liver rep2 in EODF mice sacrified at ZT16

description: every-other-day fasting (EODF)


title: H5575A

value: brain


title: C5700Cd


title: 208T

value: Radical prostatectomy


title: AB5075_control_repD [Sugar_Dminus]

city: Uxbridge

first: Rubén

last: de Dios

state: Middlesex

address: Kingston Lane

department: Life Sciences

country: United Kingdom

institute: Brunel University London

data_processing:
Demultiplexing, quality control, and adapter trimming was performed with bcl2fastq (2.20.0.422)
Read mapping was performed with HISAT
Differential expression analysis was performed using edgeR’s exact test for differences between two groups of negative-binomial counts with an estimated dispersion value of .1.
Read quantification was performed using Subread’s featureCounts functionality.
Read counts loaded into R and were normalized using edgeR’s Trimmed Mean of M values algorithm.  Subsequent values were then converted to counts per million (cpm).
Assembly: NZ_CP008706.1
Supplementary files format and content: Matrix table (tab-delimited file) with raw gene counts for every gene and every sample
Supplementary files format and content: Individual tab-delimited files with normalize counts (cpm) for every sample.

organism: Acinetobacter baumannii AB5075

source_name: Acinetobacter baumannii AB5075 (VIR-O colony morphology)

growth_protocol: A. baumannii AB5075 (6 independent replicates) was inoculated from a pure VIR-O type stock and incubated overnight in LB (37 C, 180 rpm). Then, cultures were diluted in LB (suplemented wth acesulfame K or with a mock treatment) to OD600 0.05 and incubated at 37 C, 180 rpm until reaching mid-exponential phase (OD600 0.6 approx.).

extract_protocol:
Cultures were harvested by centrifugation and washed in RNAlater to preserve RNA integrity. RNA was isolated using athe Qiagen RNAeasy Kit with on-column DNAase digestion (Qiagen). RNA quality was assessed using a Bioanalyzer.
Samples were additionally treated with RNAse-free DNAse (Invitrogen). cDNA library was prepared with Illumina’s Stranded Total RNA Prep Ligation with Ribo-Zero Plus kit and 10bp IDT for Illumina indices.

treatment_protocol: The treatment (1.33% or a water control) was applied as a supplement in the LB medium and was present since the beginning of the growth.

value: water control

tag: culture conditions

value: 42 C, 180 rpm, grown to mid- exponential phase (OD600 0.6 approx.)


title: RNA.h2o2_2h_1

value: Oxidative stress (H2O2, 2h)


title: 159T

value: No


title: RNA.h2o2_7h_2


title: AB5075_control_repC [Sugar_Cminus]

value: 41 C, 180 rpm, grown to mid- exponential phase (OD600 0.6 approx.)


title: Liver rep1 in EODF mice sacrified at ZT16

description: every-other-day fasting (EODF)


title: H5493A

tag: region

value: Nucleus Accumbens


title: Liver rep5 in AF mice sacrified at ZT16

description: acute fasting (AF)


title: 155T

tag: previous treatment

value: low


title: Liver rep4 in AF mice sacrified at ZT16

description: acute fasting (AF)


title: C5404Cin

value: Cingulate Cortex


title: RNA.h2o2_2h_2

value: Mouse embryonic fibroblasts NIH3T3


title: 141T

tag: age at_diagnosis

value: high

value: Yes


title: AB5075_control_repA [Sugar_Aminus]

value: 40 C, 180 rpm, grown to mid- exponential phase (OD600 0.6 approx.)


title: AB5075_ace-K_repE [Sugar_Eplus]

value: 1.33% acesulfame K

value: 39 C, 180 rpm, grown to mid- exponential phase (OD600 0.6 approx.)


title: Liver rep3 in AF mice sacrified at ZT16

description: acute fasting (AF)

value: Mice fasted for 4h before sacrificed


title: AB5075_ace-K_repC [Sugar_Cplus]

value: 38 C, 180 rpm, grown to mid- exponential phase (OD600 0.6 approx.)


title: Liver rep2 in AF mice sacrified at ZT16

description: acute fasting (AF)


title: C5596A

value: brain


title: H5493Cin


title: 114T

value: Prostate tumor - FFPE

value: Radical prostatectomy

tag: bmi


title: 178T

tag: erg status


title: C5382Cin


title: H5575Cin


title: 16T

tag: time to_bcr_(days)


title: AB5075_ace-K_repA [Sugar_Aplus]

value: 37 C, 180 rpm, grown to mid- exponential phase (OD600 0.6 approx.)


title: Liver rep1 in AF mice sacrified at ZT16

description: acute fasting (AF)


title: PFC24

status: Public on Oct 26 2022

city: Highland Heights

first: Brittany

middle: L

last: Smith

state: KY

address: Founders Hall

institute: Northern Kentucky University

data_processing:
BaseSpace app RNA-Seq Alignment v2.0.2 and RNA-Seq Differential Expression app version 1.0.1
STAR alignment
Salmon for assigning transcripts per million to genes and transcripts
RNA-seq Differential Expression app
Assembly: mm10
Supplementary files format and content: comma separated value .csv files containing DESeq merged gene counts

source_name: Prefrontal cortex, SAL, female, P21

growth_protocol: female C57BL/6J and male DBA/2J mice were bred to produce B6D2F1/J hybrid mice used for the study

extract_protocol:
RNA was extracted with Trizol reagent &  Qiagen RNAeasy columns. 1.0 ug of high quality total RNA was used for the construction of sequencing libraries.
PolyA RNA was enriched with SMARTer Apollo automated NGS library prep system (Takara Bio USA, Mountain View, CA) and for library preparation, NEBNext Ultra II Directional RNA Library Prep kit (New England BioLabs) was used with PCR cycle number = 8

treatment_protocol:
female C57BL/6J were treated once daily with 10mg/kg morphine sulfate (s.c.) vs. equal volume of saline for 1 week prior to gestation, throughout gestation and lactation, until offspring were weaned and euthanized on postnatal day 21
brain tissue was collected from B6D2F1/J hybrid offspring and stored in RNAlater

value: brain - prefrontal cortex

tag: genotype

value: WT

value: prenatal saline

tag: time

value: Postnatal day 21


title: PFC22


title: PFC23

source_name: Prefrontal cortex, MO, female, P21

value: prenatal morphine


title: PFC20

source_name: Prefrontal cortex, SAL, male, P21


title: PFC19

source_name: Prefrontal cortex, MO, male, P21


title: PFC17


title: PFC21


title: PFC16


title: 126T

tag: tissue

value: No

value: low

tag: gleason grade_group_at_rp

tag: clinical stage_(ct)


title: PFC15

tag: treatment


title: C5700A

value: Nucleus Accumbens


title: PFC14

tag: cell type


title: PFC13


title: PFC10


title: PFC12


title: PFC9


title: PFC8


title: PFC11


title: PFC6


title: PFC7


title: PFC5


title: PFC3


title: PFC2


title: CytoID High

city: Philadelphia

first: Joshua

middle: .H

last: Rhoades

state: PA

address: 3400 Civic Center Boulevard

institute: University of Pennsylvania

library_source: transcriptomic single cell

data_processing:
Demultiplexed with cell ranger mkfastq
Counted with cell ranger count
Processed with Seurat package, following standard workflow with addition of merging libraries and demultiplexing HTO Data.
Assembly: GRCh38-3.0.0
Supplementary files format and content: The hashtag feature reference provides the oligo sequences that correspond to each sample. Cell ranger count output feature matrices for all libraries. Seurat produced the count, normalized gene expression matrix, and hashtag oligo count matrix.

source_name: Primary tissue

extract_protocol:
Sorted cells were encapsulated using the Chromium Controller (10x Genomics) and the Chromium Single Cell 3′ Library & Gel Bead Kit (10x Genomics) following the standard manufacturer’s protocols.
All libraries were prepared for sequencing using standard Illumina protocols.
Single-cell RNA seq with HTO

value: intestinal epithelium


title: CytoID Low HTO


title: Chga-CreER eYFP HTO

data_processing:
Demultiplexed with cell ranger mkfastq
Counted with cell ranger count
Processed with Seurat package, following standard workflow with addition of demultiplexing HTO Data.
Assembly: GRCh38-3.0.0
Supplementary files format and content: The hashtag feature reference provides the oligo sequences that correspond to each sample. Cell ranger count output feature matrices for all libraries. Seurat produced the count, normalized gene expression matrix, and hashtag oligo count matrix.


title: CytoID Low


title: CytoID High HTO


title: PFC18


title: Lgr5-CreER Rep3

data_processing:
Raw sample data were mapped to the mouse reference transcriptome using kallisto (Bray et al., 2016).
Data were analyzed in the statistical computing environment R. RUVseq (Risso et al., 2014) and edgeR (McCarthy et al., 2012; Robinson et al., 2010).
Assembly: GRCh38-3.0.0

extract_protocol:
Hopx+ or Lgr5+ cells were FACS sorted into TRIzol LS (Thermo Fisher Scientific). After phase separation using the standard TRIzol LS protocol, the aqueous phase containing RNA was mixed with 70% ethanol and transferred to RNeasy MinElute spin columns (QIAGEN). The cleanup and further concentration of the RNA was done using the standard procedure of RNeasy MinElute Cleanup Kit.
The cDNA synthesis was done using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech) and the library was prepared using Nextera XT DNA Library Prep kit (Illumina).


title: Lgr5-CreER Rep2


title: Lgr5-CreER Rep1


title: Hopx-CreER Rep3


title: Hopx-CreER Rep2


title: Hopx-CreER Rep1


title: p7-2_H12

city: Daejeon

first: Hanseul

address: 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea

country: South Korea

institute: Korea Advanced Institute of Science and Technology

description: SMART Seq2 with few modification

data_processing:
Sequencing reads from single-cell RNA-Seq libraries were aligned to the mouse reference genome (version mm10 from the UCSC) using Bowtie 2 (version 2.4.2) with default parameters for paired-end reads.
Supplementary files format and content: Expression values of each gene were quantified as TPM using RSEM (version 1.3.3)

molecule: polyA RNA

source_name: colon

extract_protocol:
Colonic epithelial cells were stained with eBioscience™ Fixable Viability Dye eFluor™ 506 (65-0866-14, Invitrogen) in a 1:1000 ratio for dead cell exclusion. Cells were Fc-blocked using Purified Rat Anti-Mouse CD16/CD32 (Clone 2.4G2, 553142, BD Pharmingen™) for 10 minutes and washed with FACS buffer (Ca2+ and Mg2+-free DPBS supplemented with 0.5% BSA and 0.3% sodium azide). For surface marker staining, PE Rat Anti-Mouse Siglec-F (Clone E50-2440, 552126, BD Pharmingen™) antibody and APC anti-mouse CD326 (EpCAM) antibody (clone G8.8, 118214, Biolegend) were used in a 1:50 and 1:100 dilution ratio, respectively. Antibody staining was performed for 30 minutes. All procedures were conducted at 4°C in the dark. Tuft cells were acquired by BD FACSAriaII (BD Biosciences)
Single-cell RNA-Seq libraries were prepared using the Smart-seq2 protocol with few modifications. Single-cells were sorted using a BD FACSAriaII (BD Biosciences) into 96 well PCR plates (Thermo Scientific) containing 2μl of lysis buffer (0.1% Triton X-100, 1U/μl RNase Inhibitor (Enzynomics), 0.25μM oligo-dT30VN primer) and stored at -80°C. The plates were thawed on ice and incubated for 3 min at 72 °C to lyse the cells. Revers transcription (40U/µl Maxima H minus transcriptase), template switching reaction and PCR pre-amplification (15 cycles) were performed according to the protocol. The PCR products in each well was cleaned up using 0.5X SPRI beads (2% Sera-Mag Speed Beads (Cytiva), 1M NaCl, 10mM Tris-HCl pH 8.0, 1mM EDTA, 0.01% NP40, 0.05% Sodium Azide, 22% w/v PEG 8000). To exclude empty wells and low-quality libraries, cDNA libraries were assessed by quantitative PCR with a primer pair of GAPDH, a housekeeping gene (forward: 5′-GTCGTGGAGTCTACTGGTGTCTTCAC-3′; reverse: 5′-GTTGTCATATTTCTCGTGGTTCACACCC-3′). Samples showing a high threshold cycle (Ct) value (>35 cycles) or no PCR amplification were discarded. 50–200 pg of each cDNA library were used to generate the Illumina sequencing library using EZ-Tera XT DNA library preparation kits (Enzynomics). After the final PCR amplification, samples were pooled and size-selected by SPRI beads (removing DNA bound to 0.3X beads and taking DNA bound to subsequent 0.6X beads). Pooled sequencing libraries were sequenced on an Illumina NextSeq 500 instrument using a 38-bp paired-end reads setting.

value: colon

tag: age

value: 11 weeks old

tag: Sex

value: Female

value: VillinCre;IPMKf/f

value: tuft


title: p7-2_H11

description: SMART Seq2 with few modification


title: p7-2_H02

description: SMART Seq2 with few modification


title: p7-2_G09

description: SMART Seq2 with few modification


title: PFC1


title: p7-2_H04

description: SMART Seq2 with few modification


title: p7-2_G12

description: SMART Seq2 with few modification


title: PFC4


title: p7-2_H09

description: SMART Seq2 with few modification


title: p7-2_H08

description: SMART Seq2 with few modification


title: p7-2_H06

description: SMART Seq2 with few modification


title: p7-2_F06

description: SMART Seq2 with few modification


title: p7-2_E10

description: SMART Seq2 with few modification


title: p7-2_F08

description: SMART Seq2 with few modification


title: p7-2_G04

description: SMART Seq2 with few modification


title: p7-2_F05

description: SMART Seq2 with few modification


title: p7-2_E02

description: SMART Seq2 with few modification


title: Chga-CreER eYFP


title: p7-2_D11

description: SMART Seq2 with few modification


title: p7-2_E04

description: SMART Seq2 with few modification


title: p7-2_D04

description: SMART Seq2 with few modification


title: p7-2_D08

description: SMART Seq2 with few modification


title: p7-2_D02

description: SMART Seq2 with few modification


title: p7-2_C12

description: SMART Seq2 with few modification


title: p7-2_C09

description: SMART Seq2 with few modification


title: p7-2_C08

description: SMART Seq2 with few modification


title: p7-2_G07

description: SMART Seq2 with few modification


title: p7-2_C01

description: SMART Seq2 with few modification


title: p7-2_C03

description: SMART Seq2 with few modification


title: p7-2_B04

description: SMART Seq2 with few modification


title: p7-2_B06

description: SMART Seq2 with few modification


title: p7-2_B02

description: SMART Seq2 with few modification


title: p7-2_B03

description: SMART Seq2 with few modification


title: p7-2_E09

description: SMART Seq2 with few modification


title: p7-2_A08

description: SMART Seq2 with few modification


title: p7-2_A04

description: SMART Seq2 with few modification


title: p7-1_H12

description: SMART Seq2 with few modification


title: p7-1_H08

description: SMART Seq2 with few modification


title: p7-1_H07

description: SMART Seq2 with few modification


title: p7-1_H11

description: SMART Seq2 with few modification


title: p7-2_A10

description: SMART Seq2 with few modification


title: p7-1_H03

description: SMART Seq2 with few modification


title: p7-1_H01

description: SMART Seq2 with few modification


title: p7-1_H06

description: SMART Seq2 with few modification


title: p7-1_H02

description: SMART Seq2 with few modification


title: p7-1_G04

description: SMART Seq2 with few modification


title: p7-1_G11

description: SMART Seq2 with few modification


title: p7-1_G05

description: SMART Seq2 with few modification


title: p7-1_G08

description: SMART Seq2 with few modification


title: p7-2_C05

description: SMART Seq2 with few modification


title: p7-1_G01

description: SMART Seq2 with few modification


title: p7-1_F07

description: SMART Seq2 with few modification


title: p7-1_F06

description: SMART Seq2 with few modification


title: p7-1_F05

description: SMART Seq2 with few modification


title: p7-1_F04

description: SMART Seq2 with few modification


title: p7-2_B12

description: SMART Seq2 with few modification


title: p7-2_A06

description: SMART Seq2 with few modification


title: p7-1_F03

description: SMART Seq2 with few modification


title: p7-2_B01

description: SMART Seq2 with few modification


title: p7-1_F02

description: SMART Seq2 with few modification


title: p7-1_F01

description: SMART Seq2 with few modification


title: p7-1_E11

description: SMART Seq2 with few modification


title: p7-1_E09

description: SMART Seq2 with few modification


title: p7-1_E10

description: SMART Seq2 with few modification


title: p7-1_E06

description: SMART Seq2 with few modification


title: p7-1_D12

description: SMART Seq2 with few modification


title: p7-1_E02

description: SMART Seq2 with few modification


title: p7-1_D11

description: SMART Seq2 with few modification


title: p7-1_E05

description: SMART Seq2 with few modification


title: p7-1_D07

description: SMART Seq2 with few modification


title: p7-1_C11

description: SMART Seq2 with few modification


title: p7-1_C09

description: SMART Seq2 with few modification


title: p7-1_D05

description: SMART Seq2 with few modification


title: p7-1_D08

description: SMART Seq2 with few modification


title: p7-1_D03

description: SMART Seq2 with few modification


title: p7-1_C10

description: SMART Seq2 with few modification


title: p7-1_D04

description: SMART Seq2 with few modification


title: p7-1_C08

description: SMART Seq2 with few modification


title: p7-1_C06

description: SMART Seq2 with few modification


title: p7-1_C03

description: SMART Seq2 with few modification


title: p7-1_C07

description: SMART Seq2 with few modification


title: p7-1_C02

description: SMART Seq2 with few modification


title: p7-1_B09

description: SMART Seq2 with few modification

value: epithelial


title: p7-1_B06

description: SMART Seq2 with few modification


title: p7-1_B04

description: SMART Seq2 with few modification


title: p7-1_B03

description: SMART Seq2 with few modification


title: p7-1_A12

description: SMART Seq2 with few modification


title: p7-1_E07

description: SMART Seq2 with few modification


title: p7-1_A08

description: SMART Seq2 with few modification


title: p7-1_A11

description: SMART Seq2 with few modification


title: p7-1_A06

description: SMART Seq2 with few modification


title: p7-1_D10

description: SMART Seq2 with few modification


title: p7-1_D06

description: SMART Seq2 with few modification


title: p7-1_A03

description: SMART Seq2 with few modification


title: p6-4_H07

description: SMART Seq2 with few modification

value: wt


title: p6-4_H10

description: SMART Seq2 with few modification


title: p6-4_H08

description: SMART Seq2 with few modification


title: p6-4_H06

description: SMART Seq2 with few modification


title: p6-4_G11

description: SMART Seq2 with few modification


title: p6-4_H04

description: SMART Seq2 with few modification


title: p6-4_H05

description: SMART Seq2 with few modification


title: p6-4_H03

description: SMART Seq2 with few modification


title: p6-4_G04

description: SMART Seq2 with few modification


title: p6-4_G10

description: SMART Seq2 with few modification


title: p6-4_G07

description: SMART Seq2 with few modification


title: p6-4_G01

description: SMART Seq2 with few modification


title: p6-4_F12

description: SMART Seq2 with few modification


title: p6-4_F10

description: SMART Seq2 with few modification


title: p6-4_G02

description: SMART Seq2 with few modification


title: p6-4_G08

description: SMART Seq2 with few modification


title: p6-4_F11

description: SMART Seq2 with few modification


title: p6-4_F09

description: SMART Seq2 with few modification


title: p6-4_F06

description: SMART Seq2 with few modification


title: p6-4_F03

description: SMART Seq2 with few modification


title: p6-4_F05

description: SMART Seq2 with few modification


title: p6-4_H12

description: SMART Seq2 with few modification


title: p6-4_F01

description: SMART Seq2 with few modification


title: p6-4_E12

description: SMART Seq2 with few modification


title: p6-4_F02

description: SMART Seq2 with few modification


title: p6-4_E09

description: SMART Seq2 with few modification


title: p6-4_E10

description: SMART Seq2 with few modification


title: p6-4_E08

description: SMART Seq2 with few modification


title: p6-4_E11

description: SMART Seq2 with few modification


title: p6-4_G09

description: SMART Seq2 with few modification


title: p6-4_G03

description: SMART Seq2 with few modification


title: p6-4_E06

description: SMART Seq2 with few modification


title: p6-4_G12

description: SMART Seq2 with few modification


title: p6-4_E05

description: SMART Seq2 with few modification


title: p6-4_E07

description: SMART Seq2 with few modification


title: p6-4_E03

description: SMART Seq2 with few modification


title: p6-4_E02

description: SMART Seq2 with few modification


title: p6-4_E01

description: SMART Seq2 with few modification


title: p6-4_D10

description: SMART Seq2 with few modification


title: p6-4_D06

description: SMART Seq2 with few modification


title: p6-4_D05

description: SMART Seq2 with few modification


title: p6-4_D09

description: SMART Seq2 with few modification


title: p6-4_D03

description: SMART Seq2 with few modification


title: p6-4_D04

description: SMART Seq2 with few modification


title: p6-4_D11

description: SMART Seq2 with few modification


title: p6-4_D01

description: SMART Seq2 with few modification


title: p6-4_C12

description: SMART Seq2 with few modification


title: p6-4_D02

description: SMART Seq2 with few modification


title: p6-4_C07

description: SMART Seq2 with few modification


title: p6-4_C11

description: SMART Seq2 with few modification


title: p6-4_C10

description: SMART Seq2 with few modification


title: p6-4_C04

description: SMART Seq2 with few modification


title: p6-4_C05

description: SMART Seq2 with few modification


title: p6-4_C01

description: SMART Seq2 with few modification


title: p6-4_C02

description: SMART Seq2 with few modification


title: p7-1_A05

description: SMART Seq2 with few modification


title: p6-4_C03

description: SMART Seq2 with few modification


title: p6-4_B11

description: SMART Seq2 with few modification


title: p6-4_B12

description: SMART Seq2 with few modification


title: p6-4_B10

description: SMART Seq2 with few modification


title: p6-4_B06

description: SMART Seq2 with few modification


title: p6-4_B08

description: SMART Seq2 with few modification


title: p6-4_B09

description: SMART Seq2 with few modification


title: p6-4_B02

description: SMART Seq2 with few modification


title: p6-4_B05

description: SMART Seq2 with few modification


title: p6-4_B03

description: SMART Seq2 with few modification


title: p6-4_B01

description: SMART Seq2 with few modification


title: p6-4_A12

description: SMART Seq2 with few modification


title: p6-4_A04

description: SMART Seq2 with few modification


title: p6-4_A06

description: SMART Seq2 with few modification


title: p6-4_A09

description: SMART Seq2 with few modification


title: p6-4_A01

description: SMART Seq2 with few modification


title: p6-3_H11

description: SMART Seq2 with few modification


title: p6-4_A07

description: SMART Seq2 with few modification


title: p6-4_A10

description: SMART Seq2 with few modification


title: p6-3_H09

description: SMART Seq2 with few modification


title: p6-3_H08

description: SMART Seq2 with few modification


title: p6-4_A03

description: SMART Seq2 with few modification


title: p6-3_H04

description: SMART Seq2 with few modification


title: p6-3_G11

description: SMART Seq2 with few modification


title: p6-4_B04

description: SMART Seq2 with few modification


title: p6-3_G10

description: SMART Seq2 with few modification


title: p6-3_G12

description: SMART Seq2 with few modification


title: p6-3_G08

description: SMART Seq2 with few modification


title: p6-3_H05

description: SMART Seq2 with few modification


title: p6-3_H10

description: SMART Seq2 with few modification


title: p6-3_G06

description: SMART Seq2 with few modification


title: p6-3_H01

description: SMART Seq2 with few modification


title: p6-3_G05

description: SMART Seq2 with few modification


title: p6-3_G03

description: SMART Seq2 with few modification


title: p6-3_G04

description: SMART Seq2 with few modification


title: p6-3_F12

description: SMART Seq2 with few modification


title: p6-3_G01

description: SMART Seq2 with few modification


title: p6-3_F10

description: SMART Seq2 with few modification


title: p6-3_F09

description: SMART Seq2 with few modification


title: p6-3_F05

description: SMART Seq2 with few modification


title: p6-3_F04

description: SMART Seq2 with few modification


title: p6-3_F08

description: SMART Seq2 with few modification


title: p6-3_F01

description: SMART Seq2 with few modification


title: p6-3_G09

description: SMART Seq2 with few modification


title: p6-3_E12

description: SMART Seq2 with few modification


title: p6-3_E11

description: SMART Seq2 with few modification


title: p6-3_E09

description: SMART Seq2 with few modification


title: p6-3_E08

description: SMART Seq2 with few modification


title: p6-3_E06

description: SMART Seq2 with few modification


title: p6-3_E10

description: SMART Seq2 with few modification


title: p6-3_E02

description: SMART Seq2 with few modification


title: p6-3_F11

description: SMART Seq2 with few modification


title: p6-3_E03

description: SMART Seq2 with few modification


title: p6-3_E04

description: SMART Seq2 with few modification


title: p6-3_E05

description: SMART Seq2 with few modification


title: p6-3_E01

description: SMART Seq2 with few modification


title: p6-3_D10

description: SMART Seq2 with few modification


title: p6-3_D08

description: SMART Seq2 with few modification


title: p6-3_D09

description: SMART Seq2 with few modification


title: p6-3_D04

description: SMART Seq2 with few modification


title: p6-3_D03

description: SMART Seq2 with few modification


title: p6-3_D07

description: SMART Seq2 with few modification


title: p6-3_D02

description: SMART Seq2 with few modification


title: p6-3_C12

description: SMART Seq2 with few modification


title: p6-3_C10

description: SMART Seq2 with few modification


title: p6-3_C09

description: SMART Seq2 with few modification


title: p6-3_F03

description: SMART Seq2 with few modification


title: p6-3_C08

description: SMART Seq2 with few modification


title: p6-3_C05

description: SMART Seq2 with few modification


title: p6-3_C07

description: SMART Seq2 with few modification


title: p6-3_C04

description: SMART Seq2 with few modification


title: p6-3_C01

description: SMART Seq2 with few modification


title: p6-3_B10

description: SMART Seq2 with few modification


title: p6-3_D05

description: SMART Seq2 with few modification


title: p6-3_D12

description: SMART Seq2 with few modification


title: p6-3_B06

description: SMART Seq2 with few modification


title: p6-3_B07

description: SMART Seq2 with few modification


title: p6-3_C03

description: SMART Seq2 with few modification


title: p6-3_B09

description: SMART Seq2 with few modification


title: p6-3_B05

description: SMART Seq2 with few modification


title: p6-3_C06

description: SMART Seq2 with few modification


title: p6-3_B04

description: SMART Seq2 with few modification


title: p6-3_B08

description: SMART Seq2 with few modification


title: p6-3_D11

description: SMART Seq2 with few modification


title: p6-3_H02

description: SMART Seq2 with few modification


title: p6-3_B12

description: SMART Seq2 with few modification


title: p6-3_B01

description: SMART Seq2 with few modification


title: p6-3_A11

description: SMART Seq2 with few modification


title: p6-3_A09

description: SMART Seq2 with few modification


title: p6-3_D01

description: SMART Seq2 with few modification


title: p6-3_B02

description: SMART Seq2 with few modification


title: p6-3_A10

description: SMART Seq2 with few modification


title: p6-3_A06

description: SMART Seq2 with few modification


title: p6-3_A07

description: SMART Seq2 with few modification


title: p6-3_A05

description: SMART Seq2 with few modification


title: p6-3_A04

description: SMART Seq2 with few modification


title: p6-3_A02

description: SMART Seq2 with few modification


title: p6-3_B11

description: SMART Seq2 with few modification


title: p6-3_A01

description: SMART Seq2 with few modification


title: p6-2_H11

description: SMART Seq2 with few modification


title: p6-2_H10

description: SMART Seq2 with few modification


title: p6-2_H08

description: SMART Seq2 with few modification


title: p6-2_H09

description: SMART Seq2 with few modification


title: p6-2_H03

description: SMART Seq2 with few modification


title: p6-2_H06

description: SMART Seq2 with few modification


title: p6-2_G12

description: SMART Seq2 with few modification


title: p6-2_G09

description: SMART Seq2 with few modification


title: p6-2_G11

description: SMART Seq2 with few modification


title: p6-2_G10

description: SMART Seq2 with few modification


title: p6-2_H02

description: SMART Seq2 with few modification


title: p6-2_G08

description: SMART Seq2 with few modification


title: p6-2_G06

description: SMART Seq2 with few modification


title: p6-2_G05

description: SMART Seq2 with few modification


title: p6-2_H04

description: SMART Seq2 with few modification


title: p6-3_A08

description: SMART Seq2 with few modification


title: p6-2_G03

description: SMART Seq2 with few modification


title: p6-2_G04

description: SMART Seq2 with few modification


title: p6-2_G07

description: SMART Seq2 with few modification


title: p6-2_F06

description: SMART Seq2 with few modification


title: p6-2_G01

description: SMART Seq2 with few modification


title: p6-2_F07

description: SMART Seq2 with few modification


title: p6-2_H12

description: SMART Seq2 with few modification


title: p6-2_F04

description: SMART Seq2 with few modification


title: p6-2_F02

description: SMART Seq2 with few modification


title: p6-2_F03

description: SMART Seq2 with few modification


title: p6-2_F01

description: SMART Seq2 with few modification


title: p6-2_H07

description: SMART Seq2 with few modification


title: p6-2_E11

description: SMART Seq2 with few modification


title: p6-2_E12

description: SMART Seq2 with few modification


title: p6-2_E10

description: SMART Seq2 with few modification


title: p6-2_E09

description: SMART Seq2 with few modification


title: p6-2_E03

description: SMART Seq2 with few modification


title: p6-2_G02

description: SMART Seq2 with few modification


title: p6-2_E04

description: SMART Seq2 with few modification


title: p6-2_E02

description: SMART Seq2 with few modification


title: p6-2_D07

description: SMART Seq2 with few modification


title: p6-2_E06

description: SMART Seq2 with few modification


title: p6-2_D06

description: SMART Seq2 with few modification


title: p6-2_D08

description: SMART Seq2 with few modification


title: p6-2_E05

description: SMART Seq2 with few modification


title: p6-2_D12

description: SMART Seq2 with few modification


title: p6-2_D11

description: SMART Seq2 with few modification


title: p6-2_F10

description: SMART Seq2 with few modification


title: p6-2_D03

description: SMART Seq2 with few modification


title: p6-2_E08

description: SMART Seq2 with few modification


title: p6-2_D02

description: SMART Seq2 with few modification


title: p6-2_D01

description: SMART Seq2 with few modification


title: p6-2_D05

description: SMART Seq2 with few modification


title: p6-2_E01

description: SMART Seq2 with few modification


title: p6-2_D04

description: SMART Seq2 with few modification


title: p6-2_C10

description: SMART Seq2 with few modification


title: p6-2_C09

description: SMART Seq2 with few modification


title: p6-2_C11

description: SMART Seq2 with few modification


title: p6-2_C08

description: SMART Seq2 with few modification


title: p6-2_C12

description: SMART Seq2 with few modification


title: p6-2_C06

description: SMART Seq2 with few modification


title: p6-2_C05

description: SMART Seq2 with few modification


title: p6-2_C04

description: SMART Seq2 with few modification


title: p6-2_C02

description: SMART Seq2 with few modification


title: p6-2_F05

description: SMART Seq2 with few modification


title: p6-2_C03

description: SMART Seq2 with few modification


title: p6-2_B11

description: SMART Seq2 with few modification


title: p6-2_B09

description: SMART Seq2 with few modification


title: p6-2_B08

description: SMART Seq2 with few modification


title: p6-2_B07

description: SMART Seq2 with few modification


title: p6-2_A07

description: SMART Seq2 with few modification


title: p6-2_A09

description: SMART Seq2 with few modification


title: p6-2_B03

description: SMART Seq2 with few modification


title: p6-2_B05

description: SMART Seq2 with few modification


title: p6-2_B06

description: SMART Seq2 with few modification


title: p6-2_A05

description: SMART Seq2 with few modification


title: p6-2_A04

description: SMART Seq2 with few modification


title: p6-2_A02

description: SMART Seq2 with few modification


title: p6-2_A01

description: SMART Seq2 with few modification


title: p6-1_H10

description: SMART Seq2 with few modification


title: p6-1_H09

description: SMART Seq2 with few modification


title: p6-1_H11

description: SMART Seq2 with few modification


title: p6-1_H07

description: SMART Seq2 with few modification


title: p6-1_H06

description: SMART Seq2 with few modification


title: p6-1_H05

description: SMART Seq2 with few modification


title: p6-1_H02

description: SMART Seq2 with few modification


title: p6-2_B04

description: SMART Seq2 with few modification


title: p6-2_A03

description: SMART Seq2 with few modification


title: p6-1_H01

description: SMART Seq2 with few modification


title: p6-1_G12

description: SMART Seq2 with few modification


title: p6-2_A10

description: SMART Seq2 with few modification


title: p6-1_G11

description: SMART Seq2 with few modification


title: p6-1_H03

description: SMART Seq2 with few modification


title: p6-1_G06

description: SMART Seq2 with few modification


title: p6-1_F08

description: SMART Seq2 with few modification


title: p6-1_F05

description: SMART Seq2 with few modification


title: p6-1_F07

description: SMART Seq2 with few modification


title: p6-1_G08

description: SMART Seq2 with few modification


title: p6-1_G04

description: SMART Seq2 with few modification


title: p6-1_F04

description: SMART Seq2 with few modification


title: p6-1_F03

description: SMART Seq2 with few modification


title: p6-1_F06

description: SMART Seq2 with few modification


title: p6-1_F02

description: SMART Seq2 with few modification


title: p6-1_G05

description: SMART Seq2 with few modification


title: p6-1_F10

description: SMART Seq2 with few modification


title: p6-1_G01

description: SMART Seq2 with few modification


title: p6-1_G03

description: SMART Seq2 with few modification


title: p6-1_G02

description: SMART Seq2 with few modification


title: p6-1_E09

description: SMART Seq2 with few modification


title: p6-1_E12

description: SMART Seq2 with few modification


title: p6-1_E10

description: SMART Seq2 with few modification


title: p6-1_G10

description: SMART Seq2 with few modification


title: p6-1_F11

description: SMART Seq2 with few modification


title: p6-1_E11

description: SMART Seq2 with few modification


title: p6-1_F12

description: SMART Seq2 with few modification


title: p6-1_F09

description: SMART Seq2 with few modification


title: p6-1_E07

description: SMART Seq2 with few modification


title: p6-1_E06

description: SMART Seq2 with few modification


title: p6-1_E04

description: SMART Seq2 with few modification


title: p6-1_E03

description: SMART Seq2 with few modification


title: p6-1_E02

description: SMART Seq2 with few modification


title: p6-1_E01

description: SMART Seq2 with few modification


title: p6-1_D06

description: SMART Seq2 with few modification


title: p6-1_D05

description: SMART Seq2 with few modification


title: p6-1_D11

description: SMART Seq2 with few modification


title: p6-1_C11

description: SMART Seq2 with few modification


title: p6-1_D01

description: SMART Seq2 with few modification


title: p6-1_D08

description: SMART Seq2 with few modification


title: p6-1_D03

description: SMART Seq2 with few modification


title: p6-1_D12

description: SMART Seq2 with few modification


title: p6-1_C10

description: SMART Seq2 with few modification


title: p6-1_C09

description: SMART Seq2 with few modification


title: p6-1_D09

description: SMART Seq2 with few modification


title: p6-1_D07

description: SMART Seq2 with few modification


title: p6-1_C12

description: SMART Seq2 with few modification


title: p6-1_D10

description: SMART Seq2 with few modification


title: p6-1_C08

description: SMART Seq2 with few modification


title: p6-1_C07

description: SMART Seq2 with few modification


title: p6-2_A06

description: SMART Seq2 with few modification


title: p6-1_D04

description: SMART Seq2 with few modification


title: p6-1_C06

description: SMART Seq2 with few modification


title: p6-1_E05

description: SMART Seq2 with few modification


title: p6-1_C05

description: SMART Seq2 with few modification


title: p6-1_C04

description: SMART Seq2 with few modification


title: p6-1_C03

description: SMART Seq2 with few modification


title: p6-1_B12

description: SMART Seq2 with few modification


title: p6-1_C02

description: SMART Seq2 with few modification


title: p6-1_C01

description: SMART Seq2 with few modification


title: p6-1_B10

description: SMART Seq2 with few modification


title: p6-1_B06

description: SMART Seq2 with few modification


title: p6-1_B08

description: SMART Seq2 with few modification


title: p6-1_B04

description: SMART Seq2 with few modification


title: p6-1_A11

description: SMART Seq2 with few modification


title: p6-1_B03

description: SMART Seq2 with few modification


title: p6-1_A12

description: SMART Seq2 with few modification


title: p6-1_B01

description: SMART Seq2 with few modification


title: p6-1_B07

description: SMART Seq2 with few modification


title: p6-1_B02

description: SMART Seq2 with few modification


title: p6-1_B05

description: SMART Seq2 with few modification


title: p6-1_A10

description: SMART Seq2 with few modification


title: p6-1_A07

description: SMART Seq2 with few modification


title: p6-1_A06

description: SMART Seq2 with few modification


title: p6-2_B10

description: SMART Seq2 with few modification


title: p6-1_A05

description: SMART Seq2 with few modification


title: p6-1_A04

description: SMART Seq2 with few modification


title: p6-1_H08

description: SMART Seq2 with few modification


title: p6-1_A02

description: SMART Seq2 with few modification


title: p6-1_A01

description: SMART Seq2 with few modification


title: p6-1_B09

description: SMART Seq2 with few modification


title: p5-2_H08

description: SMART Seq2 with few modification


title: p5-2_H04

description: SMART Seq2 with few modification


title: p5-2_H11

description: SMART Seq2 with few modification


title: p5-2_H12

description: SMART Seq2 with few modification


title: p5-2_G09

description: SMART Seq2 with few modification


title: p5-2_G10

description: SMART Seq2 with few modification


title: p5-2_G01

description: SMART Seq2 with few modification


title: p5-2_G05

description: SMART Seq2 with few modification


title: p5-2_G03

description: SMART Seq2 with few modification


title: p5-2_F12

description: SMART Seq2 with few modification


title: p5-2_G08

description: SMART Seq2 with few modification


title: p5-2_H02

description: SMART Seq2 with few modification


title: p5-2_F09

description: SMART Seq2 with few modification


title: p5-2_F06

description: SMART Seq2 with few modification


title: p5-2_F10

description: SMART Seq2 with few modification


title: p5-2_F04

description: SMART Seq2 with few modification


title: p5-2_F05

description: SMART Seq2 with few modification


title: p5-2_F02

description: SMART Seq2 with few modification


title: p5-2_F03

description: SMART Seq2 with few modification


title: p5-2_H09

description: SMART Seq2 with few modification


title: p6-1_E08

description: SMART Seq2 with few modification


title: p5-2_F01

description: SMART Seq2 with few modification


title: p6-1_H12

description: SMART Seq2 with few modification


title: p5-2_E11

description: SMART Seq2 with few modification


title: p5-2_G06

description: SMART Seq2 with few modification


title: p5-2_E12

description: SMART Seq2 with few modification


title: p5-2_F08

description: SMART Seq2 with few modification


title: p5-2_H01

description: SMART Seq2 with few modification


title: p5-2_E02

description: SMART Seq2 with few modification


title: p5-2_D09

description: SMART Seq2 with few modification


title: p5-2_D06

description: SMART Seq2 with few modification


title: p5-2_E05

description: SMART Seq2 with few modification


title: p5-2_D10

description: SMART Seq2 with few modification


title: p5-2_E08

description: SMART Seq2 with few modification


title: p5-2_D05

description: SMART Seq2 with few modification


title: p5-2_D04

description: SMART Seq2 with few modification


title: p5-2_E07

description: SMART Seq2 with few modification


title: p5-2_E10

description: SMART Seq2 with few modification


title: p5-2_D08

description: SMART Seq2 with few modification


title: p5-2_C02

description: SMART Seq2 with few modification


title: p5-2_C12

description: SMART Seq2 with few modification


title: p5-2_C04

description: SMART Seq2 with few modification


title: p5-2_C11

description: SMART Seq2 with few modification


title: p5-2_C03

description: SMART Seq2 with few modification


title: p5-2_C10

description: SMART Seq2 with few modification


title: p5-2_C01

description: SMART Seq2 with few modification


title: p5-2_C09

description: SMART Seq2 with few modification


title: p5-2_D02

description: SMART Seq2 with few modification


title: p5-2_B11

description: SMART Seq2 with few modification


title: p5-2_C07

description: SMART Seq2 with few modification


title: p5-2_B09

description: SMART Seq2 with few modification


title: p5-2_B08

description: SMART Seq2 with few modification


title: p5-2_B12

description: SMART Seq2 with few modification


title: p5-2_B01

description: SMART Seq2 with few modification


title: p5-2_A10

description: SMART Seq2 with few modification


title: p5-2_B07

description: SMART Seq2 with few modification


title: p5-2_A11

description: SMART Seq2 with few modification


title: p5-2_B03

description: SMART Seq2 with few modification


title: p5-2_B06

description: SMART Seq2 with few modification


title: p5-2_A12

description: SMART Seq2 with few modification


title: p5-2_B02

description: SMART Seq2 with few modification


title: p5-2_A09

description: SMART Seq2 with few modification


title: p5-2_A08

description: SMART Seq2 with few modification


title: p5-2_A07

description: SMART Seq2 with few modification


title: p5-1_H09

description: SMART Seq2 with few modification


title: p5-2_A02

description: SMART Seq2 with few modification


title: p5-1_H10

description: SMART Seq2 with few modification


title: p5-2_A01

description: SMART Seq2 with few modification


title: p5-2_A05

description: SMART Seq2 with few modification


title: p5-1_H11

description: SMART Seq2 with few modification


title: p5-1_H12

description: SMART Seq2 with few modification


title: p5-1_H06

description: SMART Seq2 with few modification


title: p5-2_B04

description: SMART Seq2 with few modification


title: p5-2_E06

description: SMART Seq2 with few modification


title: p5-1_H04

description: SMART Seq2 with few modification


title: p5-1_H01

description: SMART Seq2 with few modification


title: p5-1_G07

description: SMART Seq2 with few modification


title: p5-1_G09

description: SMART Seq2 with few modification


title: p5-1_G08

description: SMART Seq2 with few modification


title: p5-1_G05

description: SMART Seq2 with few modification


title: p5-1_G12

description: SMART Seq2 with few modification


title: p5-1_H02

description: SMART Seq2 with few modification


title: p6-2_B01

description: SMART Seq2 with few modification


title: p5-2_A06

description: SMART Seq2 with few modification


title: p5-1_G03

description: SMART Seq2 with few modification


title: p5-1_H08

description: SMART Seq2 with few modification


title: p5-1_F12

description: SMART Seq2 with few modification


title: p5-1_F09

description: SMART Seq2 with few modification


title: p5-1_F11

description: SMART Seq2 with few modification


title: p5-1_F07

description: SMART Seq2 with few modification


title: p5-1_G02

description: SMART Seq2 with few modification


title: p5-1_G11

description: SMART Seq2 with few modification


title: p5-1_F05

description: SMART Seq2 with few modification


title: p5-1_G04

description: SMART Seq2 with few modification


title: p5-1_F10

description: SMART Seq2 with few modification


title: p5-1_F04

description: SMART Seq2 with few modification


title: p5-1_E12

description: SMART Seq2 with few modification


title: p5-1_F08

description: SMART Seq2 with few modification


title: p5-1_E11

description: SMART Seq2 with few modification


title: p5-1_F06

description: SMART Seq2 with few modification


title: p5-1_F02

description: SMART Seq2 with few modification


title: p5-1_E06

description: SMART Seq2 with few modification


title: p5-1_E07

description: SMART Seq2 with few modification


title: p5-1_F03

description: SMART Seq2 with few modification


title: p5-1_E04

description: SMART Seq2 with few modification


title: p5-1_E01

description: SMART Seq2 with few modification


title: p5-1_D09

description: SMART Seq2 with few modification


title: p5-1_D10

description: SMART Seq2 with few modification


title: p5-1_D12

description: SMART Seq2 with few modification


title: p5-1_D08

description: SMART Seq2 with few modification


title: p5-1_D07

description: SMART Seq2 with few modification


title: p5-2_D12

description: SMART Seq2 with few modification


title: p5-1_D05

description: SMART Seq2 with few modification


title: p5-1_D04

description: SMART Seq2 with few modification


title: p5-1_F01

description: SMART Seq2 with few modification


title: p5-1_E08

description: SMART Seq2 with few modification


title: p5-1_D01

description: SMART Seq2 with few modification


title: p5-1_E05

description: SMART Seq2 with few modification


title: p5-1_C08

description: SMART Seq2 with few modification


title: p5-1_C09

description: SMART Seq2 with few modification


title: p5-1_D03

description: SMART Seq2 with few modification


title: p5-1_C07

description: SMART Seq2 with few modification


title: p5-1_E10

description: SMART Seq2 with few modification


title: p5-1_C02

description: SMART Seq2 with few modification


title: p5-1_C01

description: SMART Seq2 with few modification


title: p5-1_C03

description: SMART Seq2 with few modification


title: p5-1_C05

description: SMART Seq2 with few modification


title: p5-1_B11

description: SMART Seq2 with few modification


title: p5-1_B12

description: SMART Seq2 with few modification


title: p5-1_B10

description: SMART Seq2 with few modification


title: p5-1_E03

description: SMART Seq2 with few modification


title: p5-1_B09

description: SMART Seq2 with few modification


title: p5-1_B08

description: SMART Seq2 with few modification


title: p5-1_B05

description: SMART Seq2 with few modification


title: p5-1_B04

description: SMART Seq2 with few modification


title: p5-1_A12

description: SMART Seq2 with few modification


title: p5-1_A11

description: SMART Seq2 with few modification


title: p5-1_A10

description: SMART Seq2 with few modification


title: p5-1_A09

description: SMART Seq2 with few modification


title: p5-1_B07

description: SMART Seq2 with few modification


title: p5-1_A03

description: SMART Seq2 with few modification


title: p5-1_A05

description: SMART Seq2 with few modification


title: p5-1_A04

description: SMART Seq2 with few modification


title: p4-2_H11

description: SMART Seq2 with few modification


title: p4-2_H12

description: SMART Seq2 with few modification


title: p5-1_A07

description: SMART Seq2 with few modification


title: p5-1_A06

description: SMART Seq2 with few modification


title: p4-2_H09

description: SMART Seq2 with few modification


title: p4-2_H10

description: SMART Seq2 with few modification


title: p4-2_H06

description: SMART Seq2 with few modification