title: whole blood, subject fb52e2c0, sample time point T1, library prep Plate_16

city: New York

first: Ryan

middle: Conrad

last: Thompson

state: NY

address: 1 Gustave L. Levy Place

department: Charles Bronfman Institute for Personalized Medicine

institute: Icahn School of Medicine at Mount Sinai

description: See https://www.synapse.org/#!Synapse:syn35874390/ for full clinical data, RNA-seq QC data, and other data and metadata.

data_processing:
Quality-filtered raw data was converted into FASTQ files using bcl2fastq (Illumina).
RNA-seq reads were aligned to the GRCh38 primary assembly with Gencode gene annotation v30 by STAR (v2.7.3a) using per-sample 2-pass mapping (--twopassMode Basic) and chimeric alignment options (--chimOutType Junctions SeparateSAMold -chimSegmentMin 15 -chimJunctionOverhangMin 15).
RNA-seq QC metrics were calculated by fastqc (v0.11.8) and Picard Tools (v2.22.3).
Quantification was done at the gene-level with antisense specificity using featureCounts (Subread R package v1.6.3 and strandness option -s 2) with gene-level grouping / primary alignments only / count all overlapped features (-t exon -g gene_id -primary -O).
MultiQC was used to compile and summarize per-sample statistics from STAR, Picard Tools and featureCounts (e.g. gene-level counts, mtRNA counts, globinRNA counts, etc.) into an interactive HTML report.
We excluded samples with DV200 below 80% as well as samples with fewer than 10 million mapped reads counted by featureCounts.
To remove the unwanted signal due to globin gene expression in whole blood, counts for all annotated globin genes (gene symbols CYGB, HBA1, HBA2, HBB, HBD, HBE1, HBG1, HBG2, HBM, HBQ1, HBZ, and MB) were discarded, and the remaining count matrix was transformed to counts per million (CPM).
Genes with CPM ≥1 in ≥36 samples (half the number of subjects with no positive PCR or antibody test for SARS-CoV-2 during the study period) were included in our analyses (21,194).
Gene expression was normalized for composition bias using the trimmed mean of M-values method, implemented by calcNormFactors in the edgeR package and transformed to normalized log2 CPM with observation weights computed by voomWithDreamWeights from the variancePartition package.
Batch effects were residualized from the data by fitting a linear mixed model to the normalized log2 CPM and weights for each gene with random effects for library prep plate (the batch effect to be residualized out) and blood sample ID (the biological signal to be retained) using dream, and then subtracting the best linear unbiased predictors for library prep plate while retaining the differences between blood samples and the residuals.
The technical replicates for each batch control sample were summarized to a single residualized expression value equal to the weighted mean of the technical replicates with a weight equal to the sum of the individual weights of the technical replicates.
Assembly: GRCh38 primary genome assembly with Gencode v30 gene annotation (corresponds to Ensembl version 96)
Supplementary files format and content: rnaseq_raw_count_matrix.csv: comma-delimited raw RNA-seq read counts
Supplementary files format and content: rnaseq_logCPM_matrix.csv: comma-delimited filtered, normalized, batch-corrected log2 counts per million computed by voomWithDreamWeights with values for technical replicates summarized to a single weighted mean value per blood sample
Supplementary files format and content: rnaseq_voom_weight_matrix.csv: comma-delimited voom weight matrix corresponding to normalized batch-corrected log2 CPM values

source_name: whole blood

extract_protocol:
Frozen blood samples were thawed and total RNA was extracted from the samples using a modification of the MagMax protocol for Stabilized Blood Tubes RNA Isolation Kit (Thermo Fisher, #4451893).
Samples that yielded sufficient RNA (>50 ng) were barcoded and prepared for pooled whole transcriptome sequencing using the TruSeq Stranded Total RNA Library Prep Gold (Illumina, #20020599), which is designed to remove ribosomal, globin, and mitochondrial RNA. Libraries were amplified with 15 cycles of PCR, pooled, and sequenced on a NovaSeq 6000 (Illumina) using Sprime flow cells with 100 base pair paired-end reads, targeting a mean of 50 million read pairs per sample. For a minority of samples in which the first extraction failed (N=24), RNA was re-extracted from the supernatant saved from the first centrifugation pellet. The extraction protocol was repeated starting with the second wash step after re-pelleting the RNA.

tag: tissue

value: whole blood

tag: genotype

tag: subject id

tag: blood sample_id

tag: library prep_plate

value: Female

tag: sampling time_point_label

tag: days since_first_sample

tag: patient classification_at_first_sample

tag: covid-19 positive

value: Negative


title: whole blood, subject 99eb6b42, sample time point T1, library prep Plate_19

status: Public on Dec 06 2022

type: SRA

country: USA

library_source: transcriptomic

molecule: total RNA

organism: Homo sapiens

tag: cell line

tag: cell type

tag: treatment

tag: Sex

value: Male

value: Positive


title: whole blood, subject ca128984, sample time point T2, library prep Plate_17


title: whole blood, subject eb21880d, sample time point T8, library prep Plate_19


title: whole blood, subject 318dda84, sample time point T4, library prep Plate_2


title: whole blood, subject 302cac50, sample time point T2, library prep Plate_18


title: COVID22

city: Boston

first: Jonathan

middle: David

last: Lee

state: MA

address: 3 Blackfan Circle, Rm. 424

institute: BIDMC

data_processing:
Raw sequencing reads were aligned to a reference transcriptome generated from the Ensembl v104 human transcriptome with salmon v1.4.0 using options “--seqBias --useVBOpt --gcBias --posBias --numBootstraps 30 --validateMappings”.
Assembly: hg38
Supplementary files format and content: .sf files from salmon v1.4.0. The columns represent the following:  Name: the name of the target transcript provided in the input transcript database (FASTA file).  Length: the length of the target transcript in nucleotides.  EffectiveLength: the computed effective length of the target transcript.  TPM: the estimate of the relative abundance of this transcript in units of Transcripts Per Million (TPM).  NumReads: the estimate of the number of reads mapping to each transcript that was quantified.

source_name: Frontal cortex of brain

extract_protocol:
Frozen tissue was processed using Biosafety Level 2+ (BL2+) procedures approved by the Beth Israel Deaconess Medical Center (BIDMC) Institutional Biosafety Committee (IBC). Brain tissues and primary neurons were homogenized using Trizol (Thermo Fisher) reagent and RNA extracted by phase separation. Total RNA was quantified by nanodrop (DeNovix DS-11) and TapeStation 4200 (RNA Screen Tape; Agilent Technologies, Inc).
450 ng of total RNA was used for library preparation via the KAPA RNA HyperPrep kit with RiboErase (HMR; Roche) according to the manufacturer’s recommendations. Briefly, hybridization with hybridization oligos (HMR) was performed at 95oC for 2min followed by rRNA depletion using RNAse H which was performed at 45 oC for 30min. Following rRNA cleanup via KAPA pure beads, DNase digestion was performed at 37 oC for 30min followed by cleanup, RNA elution, fragmentation (6 min at 94oC for samples with RIN ≥ 7 or 5min at 85 oC for samples with RIN ≤ 7) and priming. First and second strand synthesis and A-tailing were performed according to manufacturer’s recommendations. 1.5 uM KAPA Unique Dual-Indexed (UDI; Roche) adapters were ligated to the second strand synthesis product in the presence of a ligation master mix in a reaction that was performed at 20 oC for 15min. Following cleanup, all libraries underwent 10 cycles of amplification. Successful library production, quality control, and quantification was assessed using Tapestation (High sensitivity D1000 Screen Tape; Agilent Technologies, Inc). Libraries were pooled and sequenced via NovaSeq 6000.

tag: age

value: F

tag: matching group

value: S

tag: group


title: iPSC 102 MESO

type: genomic

city: Aachen

first: Wolfgang

last: Wagner

address: Pauwelsstrasse 20

department: Helmholtz Institute for Biomedical Engineering

country: Germany

institute: RWTH Aachen University

hyb_protocol: Bisulfite conversion and hybridization to the array was carried out by Life&Brain (Bonn, Germany). Bisulfite converted DNA was amplified, fragmented and hybridized to Illumina Infinium EPIC Beadchip using the standard protocol from Illumina.

scan_protocol: Standard protocol from Illumina.

data_processing: Analysis was performed on RAW idat files using the R package minfi and the ssNoob normalization. Detection p values were calculated using the minfi package.

label: Cy5 and Cy3

molecule: genomic DNA

source_name: iPSC

label_protocol: Standard protocol from Illumina.

growth_protocol: The iPSC lines were cultured on tissue culture plastic coated with vitronectin (0.5 µg/cm2) in StemMACS iPS-Brew XF (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany). EBs were generated following the protocol described in Ng ES, Davis RP, Azzola L, Stanley EG, Elefanty AG. Forced aggregation of defined numbers of human embryonic stem cells into embryoid bodies fosters robust, reproducible hematopoietic differentiation. Blood. 2005 Sep 1;106(5):1601-3. doi: 10.1182/blood-2005-03-0987. Epub 2005 May 24. PMID: 15914555.

extract_protocol: Genomic DNA was isolated with the NucleoSpin Tissue Kit (Macherey-Nagel, Düren, Germany).

treatment_protocol: Directed differentiation toward endodermal, mesodermal, and ectodermal lineage was induced with the STEMdiff Trilineage Differentiation Kit (Stemcell Technologies, Vancouver, Canada).

tag: gender

value: female

tag: differentiation timepoint

tag: sample group

value: mesoderm


title: whole blood, subject d7adaed4, sample time point T13, library prep Plate_15

tag: tissue

tag: genotype

tag: subject id


title: whole blood, subject ffe60b2b, sample time point T1, library prep Plate_19

tag: blood sample_id

tag: library prep_plate

tag: sampling time_point_label


title: COVID19

value: P


title: whole blood, subject ff852327, sample time point T13, library prep Plate_17

tag: days since_first_sample

tag: patient classification_at_first_sample

tag: covid-19 positive


title: HNEpC, Control_1

city: Shanghai

first: Hai

last: Lin

state: Shanghai

address: No. 600, Yishan Road, Xuhui District

country: China

institute: Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine

data_processing:
Raw reads were filtered with Seqtk to remove adaptor sequence and low-quality sequence.
Genome mapping was performed by "splices mapping" algorithm in Hista2 (version:2.0.4).
Annotation genome used was GRCh38.p4 (hg38).
Assembly: mm10
Supplementary files format and content: tab-delimited text file includes count and FPKM values for each Sample

source_name: HNEpC

growth_protocol: Cells were cultured in an airway epithelial cell basal medium (PromoCell, C-21060) with growth medium kit (PromoCell, C- 21160) in a 5% CO2 incubator at 37℃.

extract_protocol:
RNA was extracted using MagBeads Total RNA Extraction Kit（Cat#T02-096) following manufacturer's protocols.
RNA libraries for RNA-seq were prepared using VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina® following manufacturer's protocols.

treatment_protocol: Cells were treated with 2mL 100 μg/ml PM2.5 or same amount of normal culture medium for 24h.

tag: cell line

value: HNEpC

tag: cell type

value: nasal epithelial cell

tag: treatment

value: Control


title: whole blood, subject ff852327, sample time point T4, library prep Plate_7

tag: Sex


title: whole blood, subject ff852327, sample time point T12, library prep Plate_19


title: whole blood, subject ff852327, sample time point T8, library prep Plate_4


title: whole blood, subject fe62e57d, sample time point T4, library prep Plate_17


title: whole blood, subject fe62e57d, sample time point T1, library prep Plate_18


title: whole blood, subject fe62e57d, sample time point T2, library prep Plate_15


title: whole blood, subject 5d362feb, sample time point T1, library prep Plate_8


title: whole blood, subject fe5b68f0, sample time point T1, library prep Plate_15


title: whole blood, subject ff852327, sample time point T2, library prep Plate_13


title: whole blood, subject fd6471c1, sample time point T4, library prep Plate_19


title: whole blood, subject fe62e57d, sample time point T8, library prep Plate_18


title: whole blood, subject fde86321, sample time point T4, library prep Plate_10


title: whole blood, subject fd6471c1, sample time point T1, library prep Plate_8


title: whole blood, subject fcf3089a, sample time point T13, library prep Plate_14


title: whole blood, subject fcf3089a, sample time point T2, library prep Plate_3


title: whole blood, subject fcf3089a, sample time point T4, library prep Plate_5


title: whole blood, subject fcf3089a, sample time point T1, library prep Plate_2


title: whole blood, subject fcc683b5, sample time point T2, library prep Plate_5


title: whole blood, subject fcf3089a, sample time point T8, library prep Plate_5


title: whole blood, subject fd6471c1, sample time point T8, library prep Plate_14


title: whole blood, subject fc557f8f, sample time point T1, library prep Plate_19


title: Y6

tag: age

value: M

tag: matching group

tag: group

value: Young


title: whole blood, subject fc557f8f, sample time point T4, library prep Plate_8


title: whole blood, subject fcc683b5, sample time point T1, library prep Plate_7


title: whole blood, subject fc4918dc, sample time point T1, library prep Plate_18


title: whole blood, subject fba056e9, sample time point T1, library prep Plate_5


title: whole blood, subject fb907f4a, sample time point T1, library prep Plate_14


title: whole blood, subject f96fcc95, sample time point T13, library prep Plate_17


title: whole blood, subject f96fcc95, sample time point T12, library prep Plate_15


title: whole blood, subject fcf3089a, sample time point T12, library prep Plate_14


title: whole blood, subject f96fcc95, sample time point T4, library prep Plate_10


title: whole blood, subject f96fcc95, sample time point T8, library prep Plate_17


title: whole blood, subject f96fcc95, sample time point T1, library prep Plate_8


title: whole blood, subject f8a8067c, sample time point T4, library prep Plate_15


title: whole blood, subject fc557f8f, sample time point T2, library prep Plate_4


title: whole blood, subject f8a8067c, sample time point T1, library prep Plate_19


title: whole blood, subject f84c1a76, sample time point T4, library prep Plate_8


title: whole blood, subject f869206f, sample time point T1, library prep Plate_3


title: whole blood, subject f84c1a76, sample time point T2, library prep Plate_11


title: whole blood, subject f8a8067c, sample time point T2, library prep Plate_18


title: whole blood, subject f874b0ab, sample time point T0, library prep Plate_19


title: whole blood, subject f775557a, sample time point T2, library prep Plate_9


title: whole blood, subject f807b2c9, sample time point T4, library prep Plate_15


title: whole blood, subject f84c1a76, sample time point T1, library prep Plate_7


title: whole blood, subject f7199584, sample time point T1, library prep Plate_11


title: whole blood, subject f7c4a713, sample time point T1, library prep Plate_18


title: whole blood, subject f6e2911e, sample time point T1, library prep Plate_5


title: whole blood, subject f6e2911e, sample time point T4, library prep Plate_12


title: whole blood, subject f5fc1a21, sample time point T1, library prep Plate_14


title: whole blood, subject f5259cc7, sample time point T4, library prep Plate_17


title: whole blood, subject f6e2911e, sample time point T2, library prep Plate_4


title: whole blood, subject f5259cc7, sample time point T2, library prep Plate_2


title: whole blood, subject f775557a, sample time point T1, library prep Plate_11


title: whole blood, subject f5259cc7, sample time point T1, library prep Plate_2


title: whole blood, subject f3900bdf, sample time point T12, library prep Plate_18


title: whole blood, subject f3900bdf, sample time point T13, library prep Plate_16


title: whole blood, subject f3900bdf, sample time point T4, library prep Plate_8


title: whole blood, subject f3900bdf, sample time point T2, library prep Plate_9


title: whole blood, subject f3900bdf, sample time point T1, library prep Plate_9


title: whole blood, subject f38e5dca, sample time point T1, library prep Plate_5


title: whole blood, subject f38e5dca, sample time point T2, library prep Plate_7


title: whole blood, subject f32baa8f, sample time point T12, library prep Plate_7


title: whole blood, subject f32baa8f, sample time point T1, library prep Plate_2


title: whole blood, subject f28b1b6a, sample time point T1, library prep Plate_19


title: whole blood, subject f5fc1a21, sample time point T2, library prep Plate_3


title: whole blood, subject f229be6c, sample time point T13, library prep Plate_19


title: whole blood, subject f229be6c, sample time point T8, library prep Plate_18


title: whole blood, subject f20dbb4b, sample time point T1, library prep Plate_13


title: whole blood, subject f1d9c69f, sample time point T2, library prep Plate_13


title: whole blood, subject f09c9d08, sample time point T1, library prep Plate_3


title: whole blood, subject f1d9c69f, sample time point T1, library prep Plate_8


title: whole blood, subject f229be6c, sample time point T12, library prep Plate_14


title: whole blood, subject efb76cb1, sample time point T4, library prep Plate_2


title: whole blood, subject ee630923, sample time point T12, library prep Plate_19


title: whole blood, subject ee630923, sample time point T2, library prep Plate_19


title: whole blood, subject ee630923, sample time point T4, library prep Plate_16


title: whole blood, subject f0157e97, sample time point T2, library prep Plate_16


title: whole blood, subject efb76cb1, sample time point T1, library prep Plate_11


title: whole blood, subject ed553ddb, sample time point T1, library prep Plate_15


title: whole blood, subject ee630923, sample time point T13, library prep Plate_16


title: whole blood, subject ed01a4e0, sample time point T1, library prep Plate_3


title: whole blood, subject ec1ef0e4, sample time point T12, library prep Plate_8


title: whole blood, subject ecfd9917, sample time point T12, library prep Plate_8


title: whole blood, subject ecfd9917, sample time point T13, library prep Plate_11


title: whole blood, subject ec1ef0e4, sample time point T8, library prep Plate_5


title: whole blood, subject ec1ef0e4, sample time point T4, library prep Plate_16


title: whole blood, subject ebcaccc9, sample time point T8, library prep Plate_13


title: whole blood, subject ec1ef0e4, sample time point T2, library prep Plate_12


title: whole blood, subject f229be6c, sample time point T4, library prep Plate_19


title: whole blood, subject ebcaccc9, sample time point T4, library prep Plate_10


title: whole blood, subject f229be6c, sample time point T2, library prep Plate_10


title: whole blood, subject ebcaccc9, sample time point T2, library prep Plate_5


title: whole blood, subject f0157e97, sample time point T4, library prep Plate_5


title: whole blood, subject eb21880d, sample time point T13, library prep Plate_19


title: whole blood, subject ebcaccc9, sample time point T1, library prep Plate_6


title: whole blood, subject efb76cb1, sample time point T2, library prep Plate_14


title: whole blood, subject eb21880d, sample time point T12, library prep Plate_15


title: whole blood, subject ee630923, sample time point T8, library prep Plate_11


title: whole blood, subject ea1d4510, sample time point T13, library prep Plate_14


title: whole blood, subject ea1d4510, sample time point T12, library prep Plate_16


title: whole blood, subject eb21880d, sample time point T1, library prep Plate_17


title: whole blood, subject ea1d4510, sample time point T2, library prep Plate_17


title: whole blood, subject ea1d4510, sample time point T1, library prep Plate_9


title: whole blood, subject ea04d4fd, sample time point T12, library prep Plate_7


title: whole blood, subject ea04d4fd, sample time point T1, library prep Plate_12


title: whole blood, subject ea04d4fd, sample time point T4, library prep Plate_13


title: whole blood, subject ea04d4fd, sample time point T8, library prep Plate_2


title: whole blood, subject e9ea305b, sample time point T2, library prep Plate_2


title: whole blood, subject e977aca1, sample time point T2, library prep Plate_15


title: whole blood, subject e8b3f790, sample time point T4, library prep Plate_9


title: whole blood, subject e90ecc64, sample time point T2, library prep Plate_9


title: whole blood, subject e977aca1, sample time point T1, library prep Plate_9


title: whole blood, subject e8b3f790, sample time point T1, library prep Plate_2


title: whole blood, subject e8116d96, sample time point T8, library prep Plate_14


title: whole blood, subject e8b3f790, sample time point T2, library prep Plate_4


title: whole blood, subject eb4355e7, sample time point T1, library prep Plate_9


title: whole blood, subject e8116d96, sample time point T2, library prep Plate_13


title: whole blood, subject e78f97d6, sample time point T8, library prep Plate_10


title: whole blood, subject e8116d96, sample time point T1, library prep Plate_9


title: whole blood, subject e78f97d6, sample time point T12, library prep Plate_15


title: whole blood, subject eb21880d, sample time point T4, library prep Plate_16


title: whole blood, subject e78f97d6, sample time point T2, library prep Plate_4


title: whole blood, subject e78f97d6, sample time point T4, library prep Plate_5


title: whole blood, subject e78f97d6, sample time point T1, library prep Plate_15


title: whole blood, subject e718d059, sample time point T1, library prep Plate_17


title: whole blood, subject e707a5ca, sample time point T8, library prep Plate_3


title: whole blood, subject e707a5ca, sample time point T12, library prep Plate_11


title: whole blood, subject e687040d, sample time point T12, library prep Plate_16


title: whole blood, subject ea1d4510, sample time point T8, library prep Plate_17


title: whole blood, subject e687040d, sample time point T8, library prep Plate_8


title: whole blood, subject e687040d, sample time point T4, library prep Plate_8


title: whole blood, subject e707a5ca, sample time point T2, library prep Plate_7


title: whole blood, subject e687040d, sample time point T2, library prep Plate_5


title: whole blood, subject e687040d, sample time point T1, library prep Plate_17


title: whole blood, subject e4e3789a, sample time point T13, library prep Plate_18


title: whole blood, subject e5865047, sample time point T1B, library prep Plate_11


title: whole blood, subject e4e3789a, sample time point T12, library prep Plate_16


title: whole blood, subject e4e3789a, sample time point T2, library prep Plate_9


title: whole blood, subject e53e70d3, sample time point T2, library prep Plate_14


title: whole blood, subject e4e3789a, sample time point T8, library prep Plate_18


title: whole blood, subject e4729ab6, sample time point T4, library prep Plate_8


title: whole blood, subject e3dd4350, sample time point T1, library prep Plate_7


title: whole blood, subject e3d686b8, sample time point T12, library prep Plate_6


title: whole blood, subject e3d686b8, sample time point T8, library prep Plate_4


title: whole blood, subject e34fa597, sample time point T1, library prep Plate_3


title: whole blood, subject e707a5ca, sample time point T4, library prep Plate_8


title: whole blood, subject e3d686b8, sample time point T2, library prep Plate_2


title: whole blood, subject e3d686b8, sample time point T4, library prep Plate_15


title: whole blood, subject e3d686b8, sample time point T13, library prep Plate_14


title: whole blood, subject e28e61f1, sample time point T13, library prep Plate_15


title: whole blood, subject e34fa597, sample time point T2, library prep Plate_16


title: whole blood, subject e28e61f1, sample time point T12, library prep Plate_19


title: whole blood, subject e34fa597, sample time point T4, library prep Plate_5


title: whole blood, subject e28e61f1, sample time point T2, library prep Plate_19


title: whole blood, subject e28e61f1, sample time point T8, library prep Plate_18


title: whole blood, subject e28e61f1, sample time point T1, library prep Plate_19


title: whole blood, subject e19edd6e, sample time point T12, library prep Plate_19


title: whole blood, subject e1e3b175, sample time point T12, library prep Plate_4


title: whole blood, subject e1e3b175, sample time point T8, library prep Plate_4


title: whole blood, subject e4729ab6, sample time point T2, library prep Plate_14


title: whole blood, subject e1015f47, sample time point T4, library prep Plate_7


title: whole blood, subject e1015f47, sample time point T8, library prep Plate_5


title: whole blood, subject e4729ab6, sample time point T1, library prep Plate_7


title: whole blood, subject e1015f47, sample time point T1, library prep Plate_15


title: whole blood, subject e1015f47, sample time point T2, library prep Plate_3


title: whole blood, subject e3dd4350, sample time point T2, library prep Plate_14


title: whole blood, subject e0e8f34b, sample time point T12, library prep Plate_2


title: whole blood, subject e0e8f34b, sample time point T2, library prep Plate_14


title: whole blood, subject de78995d, sample time point T1, library prep Plate_10


title: whole blood, subject de78995d, sample time point T2, library prep Plate_2


title: whole blood, subject de607d2b, sample time point T2, library prep Plate_4


title: whole blood, subject de607d2b, sample time point T8, library prep Plate_19


title: whole blood, subject de607d2b, sample time point T1, library prep Plate_9


title: whole blood, subject de46f293, sample time point T12, library prep Plate_13


title: whole blood, subject de607d2b, sample time point T4, library prep Plate_6


title: whole blood, subject e28e61f1, sample time point T4, library prep Plate_17


title: whole blood, subject de46f293, sample time point T4, library prep Plate_8


title: whole blood, subject e1e3b175, sample time point T4, library prep Plate_17


title: whole blood, subject de46f293, sample time point T8, library prep Plate_9


title: whole blood, subject de46f293, sample time point T1, library prep Plate_8


title: whole blood, subject dd383e46, sample time point T8, library prep Plate_14


title: whole blood, subject dd383e46, sample time point T2, library prep Plate_8


title: whole blood, subject dd383e46, sample time point T12, library prep Plate_16


title: whole blood, subject e0e8f34b, sample time point T8, library prep Plate_5


title: whole blood, subject dd383e46, sample time point T4, library prep Plate_17


title: whole blood, subject dc557cb4, sample time point T12, library prep Plate_2


title: whole blood, subject dcce851f, sample time point T1, library prep Plate_11


title: whole blood, subject dc31ed75, sample time point T2, library prep Plate_4


title: whole blood, subject dc557cb4, sample time point T1, library prep Plate_10


title: whole blood, subject e0e8f34b, sample time point T4, library prep Plate_14


title: whole blood, subject dc557cb4, sample time point T2, library prep Plate_2


title: whole blood, subject dc557cb4, sample time point T13, library prep Plate_2


title: whole blood, subject dc0bc1f9, sample time point T8, library prep Plate_16


title: whole blood, subject dc31ed75, sample time point T0, library prep Plate_14


title: whole blood, subject dc0bc1f9, sample time point T12, library prep Plate_6


title: whole blood, subject db8b671f, sample time point T13, library prep Plate_17


title: whole blood, subject dc0bc1f9, sample time point T1, library prep Plate_2


title: whole blood, subject dd383e46, sample time point T13, library prep Plate_18


title: whole blood, subject db8b671f, sample time point T1, library prep Plate_18


title: whole blood, subject db8b671f, sample time point T12, library prep Plate_8


title: whole blood, subject db803103, sample time point T4, library prep Plate_2


title: whole blood, subject db803103, sample time point T8, library prep Plate_13


title: whole blood, subject dcce851f, sample time point T2, library prep Plate_16


title: whole blood, subject db0ff43f, sample time point T2, library prep Plate_17


title: whole blood, subject db3a573f, sample time point T4, library prep Plate_14


title: whole blood, subject db0ff43f, sample time point T4, library prep Plate_14


title: whole blood, subject db0ff43f, sample time point T13, library prep Plate_18


title: whole blood, subject db0ff43f, sample time point T8, library prep Plate_14


title: whole blood, subject db3a573f, sample time point T1, library prep Plate_11


title: whole blood, subject da3d1cb6, sample time point T2, library prep Plate_12


title: whole blood, subject da3d1cb6, sample time point T8, library prep Plate_17


title: whole blood, subject da3d1cb6, sample time point T4, library prep Plate_10


title: whole blood, subject db0ff43f, sample time point T1, library prep Plate_17


title: whole blood, subject da3d1cb6, sample time point T12, library prep Plate_18


title: whole blood, subject da24293e, sample time point T4, library prep Plate_10


title: whole blood, subject da24293e, sample time point T8, library prep Plate_4


title: whole blood, subject da24293e, sample time point T2, library prep Plate_6


title: whole blood, subject d9bb1b6a, sample time point T4, library prep Plate_8


title: whole blood, subject da24293e, sample time point T1, library prep Plate_3


title: whole blood, subject da3d1cb6, sample time point T1, library prep Plate_6


title: whole blood, subject d8028ee0, sample time point T1, library prep Plate_7


title: whole blood, subject d7f3c9ee, sample time point T4, library prep Plate_12


title: whole blood, subject d9bb1b6a, sample time point T2, library prep Plate_9


title: whole blood, subject d7f3c9ee, sample time point T2, library prep Plate_4


title: whole blood, subject d7f3c9ee, sample time point T1, library prep Plate_13


title: whole blood, subject db0ff43f, sample time point T0, library prep Plate_19


title: whole blood, subject d7adaed4, sample time point T8, library prep Plate_19


title: whole blood, subject d7adaed4, sample time point T12, library prep Plate_18


title: whole blood, subject d78e965a, sample time point T8, library prep Plate_17


title: whole blood, subject d78e965a, sample time point T12, library prep Plate_14


title: whole blood, subject d7adaed4, sample time point T4, library prep Plate_18


title: whole blood, subject d78e965a, sample time point T13, library prep Plate_15


title: whole blood, subject d78770a0, sample time point T2, library prep Plate_15


title: whole blood, subject d78e965a, sample time point T4, library prep Plate_10


title: whole blood, subject d78770a0, sample time point T8, library prep Plate_19


title: whole blood, subject d78770a0, sample time point T12, library prep Plate_19


title: whole blood, subject d78e965a, sample time point T1, library prep Plate_7


title: whole blood, subject d78770a0, sample time point T4, library prep Plate_15


title: whole blood, subject d703e6ae, sample time point T2, library prep Plate_19


title: whole blood, subject d78770a0, sample time point T1, library prep Plate_19


title: whole blood, subject d703e6ae, sample time point T4, library prep Plate_12


title: whole blood, subject d6c2f748, sample time point T4, library prep Plate_6


title: whole blood, subject d703e6ae, sample time point T1, library prep Plate_8


title: whole blood, subject d6c2f748, sample time point T1, library prep Plate_9


title: whole blood, subject d8028ee0, sample time point T2, library prep Plate_3


title: whole blood, subject d683ade0, sample time point T12, library prep Plate_13


title: whole blood, subject d683ade0, sample time point T8, library prep Plate_10


title: whole blood, subject d650fa11, sample time point T12, library prep Plate_17


title: whole blood, subject d650fa11, sample time point T4, library prep Plate_5


title: whole blood, subject d5ffe2b1, sample time point T2, library prep Plate_10


title: whole blood, subject d55887ec, sample time point T1, library prep Plate_11


title: whole blood, subject d4a362f0, sample time point T12, library prep Plate_14


title: whole blood, subject d4a362f0, sample time point T13, library prep Plate_19


title: whole blood, subject d4a362f0, sample time point T8, library prep Plate_17


title: whole blood, subject d4a362f0, sample time point T2, library prep Plate_8


title: whole blood, subject d4840c8b, sample time point T4, library prep Plate_12


title: whole blood, subject d4a362f0, sample time point T1, library prep Plate_2


title: whole blood, subject d4840c8b, sample time point T8, library prep Plate_18


title: whole blood, subject d4840c8b, sample time point T2, library prep Plate_7


title: whole blood, subject d4840c8b, sample time point T1, library prep Plate_3


title: whole blood, subject d4840c8b, sample time point T12, library prep Plate_19


title: whole blood, subject d4a362f0, sample time point T4, library prep Plate_9


title: whole blood, subject d683ade0, sample time point T13, library prep Plate_16


title: whole blood, subject d463b069, sample time point T4, library prep Plate_16


title: whole blood, subject d42085cf, sample time point T13, library prep Plate_7


title: whole blood, subject d463b069, sample time point T2, library prep Plate_6


title: whole blood, subject d463b069, sample time point T8, library prep Plate_8


title: whole blood, subject d4626a9b, sample time point T1, library prep Plate_13


title: whole blood, subject d3aa54b3, sample time point T2, library prep Plate_7


title: whole blood, subject d3aa54b3, sample time point T4, library prep Plate_18


title: whole blood, subject d463b069, sample time point T1, library prep Plate_4


title: whole blood, subject d3aa54b3, sample time point T0, library prep Plate_3


title: whole blood, subject d3aa54b3, sample time point T1, library prep Plate_4


title: whole blood, subject d2b3daf4, sample time point T12, library prep Plate_19


title: whole blood, subject d2b3daf4, sample time point T8, library prep Plate_14


title: whole blood, subject d28718f3, sample time point T12, library prep Plate_13


title: whole blood, subject d2b3daf4, sample time point T4, library prep Plate_19


title: whole blood, subject d28718f3, sample time point T8, library prep Plate_7


title: whole blood, subject d2b3daf4, sample time point T2, library prep Plate_2


title: whole blood, subject d28718f3, sample time point T1, library prep Plate_4


title: whole blood, subject d28718f3, sample time point T2, library prep Plate_2


title: whole blood, subject d28718f3, sample time point T4, library prep Plate_4


title: whole blood, subject d28718f3, sample time point T0, library prep Plate_5


title: whole blood, subject d2649ca8, sample time point T4, library prep Plate_12


title: whole blood, subject d3aa54b3, sample time point T8, library prep Plate_5


title: whole blood, subject d2649ca8, sample time point T2, library prep Plate_6


title: whole blood, subject d15ed14d, sample time point T1, library prep Plate_2


title: whole blood, subject d1ea06c3, sample time point T2, library prep Plate_5


title: whole blood, subject d15ed14d, sample time point T2, library prep Plate_10


title: whole blood, subject d1dc29d4, sample time point T2, library prep Plate_9


title: whole blood, subject d0544d28, sample time point T1, library prep Plate_12


title: whole blood, subject d2649ca8, sample time point T1, library prep Plate_8


title: whole blood, subject d2b3daf4, sample time point T1, library prep Plate_3


title: whole blood, subject cfa8b753, sample time point T1, library prep Plate_2


title: whole blood, subject d01d4d62, sample time point T8, library prep Plate_11


title: whole blood, subject d01d4d62, sample time point T1, library prep Plate_5


title: whole blood, subject cfa8b753, sample time point T4, library prep Plate_6


title: whole blood, subject ce7d0642, sample time point T4, library prep Plate_13


title: whole blood, subject d01d4d62, sample time point T4, library prep Plate_3


title: whole blood, subject cfa8b753, sample time point T0, library prep Plate_12


title: whole blood, subject ce7d0642, sample time point T2, library prep Plate_15


title: whole blood, subject d0544d28, sample time point T0, library prep Plate_2


title: whole blood, subject cec04d04, sample time point T1, library prep Plate_15


title: whole blood, subject cdff3ced, sample time point T2, library prep Plate_7


title: whole blood, subject ce17b133, sample time point T1, library prep Plate_17


title: whole blood, subject ce7d0642, sample time point T1, library prep Plate_9


title: whole blood, subject cdff3ced, sample time point T4, library prep Plate_18


title: whole blood, subject cdff3ced, sample time point T1, library prep Plate_12


title: whole blood, subject cde00319, sample time point T13, library prep Plate_10


title: whole blood, subject cde00319, sample time point T12, library prep Plate_11


title: whole blood, subject cde00319, sample time point T3, library prep Plate_5


title: whole blood, subject cde00319, sample time point T8, library prep Plate_2


title: whole blood, subject cde00319, sample time point T1, library prep Plate_14


title: whole blood, subject cda38947, sample time point T2, library prep Plate_3


title: whole blood, subject cda38947, sample time point T1, library prep Plate_2


title: whole blood, subject cec04d04, sample time point T2, library prep Plate_16


title: whole blood, subject cd79de3c, sample time point T13, library prep Plate_18


title: whole blood, subject cd79de3c, sample time point T12, library prep Plate_15


title: whole blood, subject cfa8b753, sample time point T2, library prep Plate_11


title: whole blood, subject cd79de3c, sample time point T1, library prep Plate_17


title: whole blood, subject cd79de3c, sample time point T8, library prep Plate_17


title: whole blood, subject ccce11a7, sample time point T4, library prep Plate_5


title: whole blood, subject cb70545d, sample time point T1, library prep Plate_4


title: whole blood, subject ce17b133, sample time point T4, library prep Plate_3


title: whole blood, subject cb3e883f, sample time point T4, library prep Plate_11


title: whole blood, subject cb3e883f, sample time point T8, library prep Plate_10


title: whole blood, subject ccce11a7, sample time point T2, library prep Plate_3


title: whole blood, subject ccd360db, sample time point T2, library prep Plate_2


title: whole blood, subject cb3e883f, sample time point T2, library prep Plate_4


title: whole blood, subject cb1296c7, sample time point T2, library prep Plate_6


title: whole blood, subject cb1296c7, sample time point T8, library prep Plate_11


title: whole blood, subject cb1296c7, sample time point T4, library prep Plate_9


title: whole blood, subject cb1296c7, sample time point T1, library prep Plate_19


title: whole blood, subject c927cf0e, sample time point T1, library prep Plate_18


title: whole blood, subject c8ff0a0d, sample time point T13, library prep Plate_14


title: whole blood, subject c8ff0a0d, sample time point T8, library prep Plate_3


title: whole blood, subject cd79de3c, sample time point T4, library prep Plate_14


title: whole blood, subject ca128984, sample time point T1, library prep Plate_14


title: whole blood, subject c8719b33, sample time point T4, library prep Plate_9


title: whole blood, subject c8ff0a0d, sample time point T1, library prep Plate_7


title: whole blood, subject c8719b33, sample time point T8, library prep Plate_11


title: whole blood, subject c8719b33, sample time point T2, library prep Plate_10


title: whole blood, subject c8ff0a0d, sample time point T4, library prep Plate_7


title: whole blood, subject c82d0824, sample time point T2, library prep Plate_14


title: whole blood, subject c82d0824, sample time point T1, library prep Plate_5


title: whole blood, subject c8ff0a0d, sample time point T2, library prep Plate_17


title: whole blood, subject c7ad26db, sample time point T4, library prep Plate_6


title: whole blood, subject c8719b33, sample time point T1, library prep Plate_2


title: whole blood, subject c7ad26db, sample time point T2, library prep Plate_6


title: whole blood, subject c65c02dd, sample time point T2, library prep Plate_3


title: whole blood, subject c7ad26db, sample time point T1, library prep Plate_12


title: whole blood, subject c665f523, sample time point T4, library prep Plate_6


title: whole blood, subject c78052dd, sample time point T1, library prep Plate_19


title: whole blood, subject c76458ce, sample time point T1, library prep Plate_19


title: whole blood, subject c59bd348, sample time point T2, library prep Plate_14


title: whole blood, subject c5988ee0, sample time point T1, library prep Plate_17


title: whole blood, subject c665f523, sample time point T1, library prep Plate_4


title: whole blood, subject c3797258, sample time point T1, library prep Plate_16


title: whole blood, subject c65c02dd, sample time point T4, library prep Plate_3


title: whole blood, subject c65c02dd, sample time point T1, library prep Plate_3


title: whole blood, subject c3797258, sample time point T1, library prep Plate_12


title: whole blood, subject c59bd348, sample time point T8, library prep Plate_14


title: whole blood, subject c3797258, sample time point T1, library prep Plate_6


title: whole blood, subject c359e5ca, sample time point T1, library prep Plate_15


title: whole blood, subject c927cf0e, sample time point T2, library prep Plate_4


title: whole blood, subject c34042b9, sample time point T4, library prep Plate_12


title: whole blood, subject c2ab97d4, sample time point T13, library prep Plate_9


title: whole blood, subject c8ff0a0d, sample time point T12, library prep Plate_10


title: whole blood, subject c3797258, sample time point T1, library prep Plate_2


title: whole blood, subject c3797258, sample time point T1, library prep Plate_4


title: whole blood, subject c59bd348, sample time point T4, library prep Plate_8


title: whole blood, subject c3797258, sample time point T1, library prep Plate_18


title: whole blood, subject c58f203a, sample time point T1, library prep Plate_19


title: whole blood, subject c3797258, sample time point T1, library prep Plate_14


title: whole blood, subject c2ab97d4, sample time point T4, library prep Plate_6


title: whole blood, subject c3797258, sample time point T1, library prep Plate_10


title: whole blood, subject c3797258, sample time point T1, library prep Plate_8


title: whole blood, subject c24d17c6, sample time point T1, library prep Plate_15


title: whole blood, subject c12a975c, sample time point T4, library prep Plate_3


title: whole blood, subject c06f90b7, sample time point T12, library prep Plate_18


title: whole blood, subject c02cee4e, sample time point T1, library prep Plate_13


title: whole blood, subject c06f90b7, sample time point T1, library prep Plate_12


title: whole blood, subject c06f90b7, sample time point T8, library prep Plate_10


title: whole blood, subject bfb9f0d5, sample time point T13, library prep Plate_15


title: whole blood, subject c02cee4e, sample time point T4, library prep Plate_6


title: whole blood, subject bfb9f0d5, sample time point T8, library prep Plate_18


title: whole blood, subject c06f90b7, sample time point T2, library prep Plate_19


title: whole blood, subject c34042b9, sample time point T2, library prep Plate_7


title: whole blood, subject bfb9f0d5, sample time point T4, library prep Plate_8


title: whole blood, subject bfb9f0d5, sample time point T1, library prep Plate_7


title: whole blood, subject bfb9f0d5, sample time point T2, library prep Plate_9


title: whole blood, subject bf2bf92b, sample time point T2, library prep Plate_3


title: whole blood, subject bf04cb82, sample time point T1, library prep Plate_9


title: whole blood, subject bf04cb82, sample time point T12, library prep Plate_16


title: whole blood, subject bf04cb82, sample time point T4, library prep Plate_10


title: whole blood, subject be3bd991, sample time point T4, library prep Plate_17


title: whole blood, subject bf04cb82, sample time point T13, library prep Plate_17


title: whole blood, subject bece8414, sample time point T2, library prep Plate_12


title: whole blood, subject bece8414, sample time point T1, library prep Plate_9


title: whole blood, subject be3bd991, sample time point T2, library prep Plate_6


title: whole blood, subject bdb8a81c, sample time point T8, library prep Plate_14


title: whole blood, subject bdb8a81c, sample time point T4, library prep Plate_8


title: whole blood, subject bf04cb82, sample time point T8, library prep Plate_19


title: whole blood, subject bdb8a81c, sample time point T1, library prep Plate_18


title: whole blood, subject bc367dea, sample time point T13, library prep Plate_16


title: whole blood, subject bca30fba, sample time point T2, library prep Plate_5


title: whole blood, subject bca30fba, sample time point T1, library prep Plate_3


title: whole blood, subject bc367dea, sample time point T8, library prep Plate_6


title: whole blood, subject bc367dea, sample time point T4, library prep Plate_7


title: whole blood, subject bc367dea, sample time point T2, library prep Plate_13


title: whole blood, subject bbe4f261, sample time point T4, library prep Plate_12


title: whole blood, subject bfb9f0d5, sample time point T12, library prep Plate_15


title: whole blood, subject bbc4f6df, sample time point T2, library prep Plate_12


title: whole blood, subject b974b122, sample time point T2, library prep Plate_14


title: whole blood, subject b974b122, sample time point T4, library prep Plate_16


title: whole blood, subject b974b122, sample time point T1, library prep Plate_16


title: whole blood, subject bdb8a81c, sample time point T2, library prep Plate_11


title: whole blood, subject b8a6551d, sample time point T2, library prep Plate_11


title: whole blood, subject b8a6551d, sample time point T1, library prep Plate_17


title: whole blood, subject b82ddf4f, sample time point T2, library prep Plate_10


title: whole blood, subject b8414bb0, sample time point T8, library prep Plate_3


title: whole blood, subject b8414bb0, sample time point T2, library prep Plate_3


title: whole blood, subject b82ddf4f, sample time point T1, library prep Plate_16


title: whole blood, subject bf2bf92b, sample time point T1, library prep Plate_7


title: whole blood, subject b8414bb0, sample time point T12, library prep Plate_17


title: whole blood, subject bc367dea, sample time point T12, library prep Plate_7


title: whole blood, subject b8414bb0, sample time point T1, library prep Plate_2


title: whole blood, subject b7c739c9, sample time point T4, library prep Plate_3


title: whole blood, subject b7c739c9, sample time point T1, library prep Plate_8


title: whole blood, subject b7c739c9, sample time point T12, library prep Plate_12


title: whole blood, subject b77aa144, sample time point T12, library prep Plate_15


title: whole blood, subject b7c69374, sample time point T2, library prep Plate_9


title: whole blood, subject b77aa144, sample time point T8, library prep Plate_14


title: whole blood, subject b7c69374, sample time point T12, library prep Plate_11


title: whole blood, subject bbe4f261, sample time point T1, library prep Plate_5


title: whole blood, subject bbe4f261, sample time point T2, library prep Plate_13


title: whole blood, subject bbc4f6df, sample time point T1, library prep Plate_11


title: whole blood, subject b9c7c078, sample time point T1, library prep Plate_15


title: whole blood, subject b77aa144, sample time point T4, library prep Plate_14


title: whole blood, subject b77aa144, sample time point T2, library prep Plate_7


title: whole blood, subject b7726c1a, sample time point T4, library prep Plate_5


title: whole blood, subject b7726c1a, sample time point T1, library prep Plate_2


title: whole blood, subject b741da89, sample time point T8, library prep Plate_13


title: whole blood, subject b7726c1a, sample time point T2, library prep Plate_8


title: whole blood, subject b741da89, sample time point T4, library prep Plate_2


title: whole blood, subject b6d73287, sample time point T1, library prep Plate_12


title: whole blood, subject b7c69374, sample time point T1, library prep Plate_19


title: whole blood, subject b43f71cb, sample time point T2, library prep Plate_10


title: whole blood, subject b5095d08, sample time point T4, library prep Plate_19


title: whole blood, subject b43f71cb, sample time point T1, library prep Plate_8


title: whole blood, subject b5095d08, sample time point T2, library prep Plate_6


title: whole blood, subject b4cf1b9d, sample time point T1, library prep Plate_12


title: whole blood, subject b43f71cb, sample time point T4, library prep Plate_16


title: whole blood, subject b0b02bb8, sample time point T8, library prep Plate_11


title: whole blood, subject b0d5ae31, sample time point T1, library prep Plate_14


title: whole blood, subject b0d5ae31, sample time point T4, library prep Plate_4


title: whole blood, subject b0b02bb8, sample time point T13, library prep Plate_18


title: whole blood, subject b0d5ae31, sample time point T8, library prep Plate_7


title: whole blood, subject b0b02bb8, sample time point T2, library prep Plate_6


title: whole blood, subject b77aa144, sample time point T1, library prep Plate_12


title: whole blood, subject af7e42f1, sample time point T1, library prep Plate_11


title: whole blood, subject b0d5ae31, sample time point T12, library prep Plate_15


title: whole blood, subject b061d79c, sample time point T1, library prep Plate_2


title: whole blood, subject b0b02bb8, sample time point T1, library prep Plate_12


title: whole blood, subject af4f750a, sample time point T8, library prep Plate_6


title: whole blood, subject af4f750a, sample time point T13, library prep Plate_14


title: whole blood, subject b18b35ec, sample time point T2, library prep Plate_5


title: whole blood, subject af4f750a, sample time point T12, library prep Plate_12


title: whole blood, subject b0d5ae31, sample time point T2, library prep Plate_9


title: whole blood, subject af4f750a, sample time point T1, library prep Plate_15


title: whole blood, subject af4f750a, sample time point T2, library prep Plate_5


title: whole blood, subject ae4eb9bd, sample time point T8, library prep Plate_10


title: whole blood, subject af4f750a, sample time point T0, library prep Plate_14


title: whole blood, subject b0d5ae31, sample time point T13, library prep Plate_19


title: whole blood, subject ae4eb9bd, sample time point T4, library prep Plate_16


title: whole blood, subject ae4eb9bd, sample time point T1, library prep Plate_4


title: whole blood, subject ae4eb9bd, sample time point T2, library prep Plate_3


title: whole blood, subject aba21b12, sample time point T8, library prep Plate_17


title: whole blood, subject aba21b12, sample time point T4, library prep Plate_12


title: whole blood, subject aba21b12, sample time point T2, library prep Plate_19


title: whole blood, subject aa853c05, sample time point T8, library prep Plate_3


title: whole blood, subject aa853c05, sample time point T2, library prep Plate_3


title: whole blood, subject aa499b5b, sample time point T2, library prep Plate_10


title: whole blood, subject aa853c05, sample time point T1, library prep Plate_10


title: whole blood, subject a9f46132, sample time point T4, library prep Plate_6


title: whole blood, subject a9f46132, sample time point T2, library prep Plate_16


title: whole blood, subject a9f46132, sample time point T8, library prep Plate_7


title: whole blood, subject af4f750a, sample time point T4, library prep Plate_4


title: whole blood, subject a8f43017, sample time point T12, library prep Plate_18


title: whole blood, subject a8f43017, sample time point T8, library prep Plate_8


title: whole blood, subject a8f43017, sample time point T4, library prep Plate_6


title: whole blood, subject a7d130f6, sample time point T1, library prep Plate_16


title: whole blood, subject a6f6c40c, sample time point T1, library prep Plate_10


title: whole blood, subject a7b87e83, sample time point T2, library prep Plate_7


title: whole blood, subject a62483bb, sample time point T1, library prep Plate_14


title: whole blood, subject a63504b7, sample time point T1, library prep Plate_6


title: whole blood, subject acc5de3c, sample time point T5, library prep Plate_14


title: whole blood, subject a5b7cb76, sample time point T1, library prep Plate_16


title: whole blood, subject a56fd839, sample time point T3, library prep Plate_19


title: whole blood, subject aba21b12, sample time point T1, library prep Plate_5


title: whole blood, subject a56fd839, sample time point T4, library prep Plate_8


title: whole blood, subject a56fd839, sample time point T1, library prep Plate_13


title: whole blood, subject a4cb2478, sample time point T12, library prep Plate_8


title: whole blood, subject a4cb2478, sample time point T8, library prep Plate_11


title: whole blood, subject a4a38a22, sample time point T8, library prep Plate_7


title: whole blood, subject a4cb2478, sample time point T4, library prep Plate_19


title: whole blood, subject a8f43017, sample time point T13, library prep Plate_16


title: whole blood, subject a7d130f6, sample time point T4, library prep Plate_4


title: whole blood, subject a4bf14db, sample time point T2, library prep Plate_8


title: whole blood, subject a4a38a22, sample time point T4, library prep Plate_17


title: whole blood, subject a7d130f6, sample time point T8, library prep Plate_4


title: whole blood, subject a4a38a22, sample time point T2, library prep Plate_10


title: whole blood, subject a4bf14db, sample time point T8, library prep Plate_10


title: whole blood, subject a48e192e, sample time point T2, library prep Plate_11


title: whole blood, subject a48e192e, sample time point T4, library prep Plate_3


title: whole blood, subject a414431c, sample time point T12, library prep Plate_17


title: whole blood, subject a414431c, sample time point T8, library prep Plate_6


title: whole blood, subject a472cc4b, sample time point T2, library prep Plate_9


title: whole blood, subject a472cc4b, sample time point T12, library prep Plate_15


title: whole blood, subject a48e192e, sample time point T1, library prep Plate_19


title: whole blood, subject a46725b1, sample time point T2, library prep Plate_5


title: whole blood, subject b0b02bb8, sample time point T4, library prep Plate_6


title: whole blood, subject a4cb2478, sample time point T13, library prep Plate_17


title: whole blood, subject a414431c, sample time point T4, library prep Plate_5


title: whole blood, subject a395f41c, sample time point T2, library prep Plate_9


title: whole blood, subject a4cb2478, sample time point T1, library prep Plate_9


title: whole blood, subject a414431c, sample time point T1, library prep Plate_2


title: whole blood, subject a8f43017, sample time point T1, library prep Plate_17


title: whole blood, subject a395f41c, sample time point T4, library prep Plate_13


title: whole blood, subject a414431c, sample time point T2, library prep Plate_3


title: whole blood, subject a395f41c, sample time point T1, library prep Plate_2


title: whole blood, subject a2f4eb0b, sample time point T8, library prep Plate_2


title: whole blood, subject a2f4eb0b, sample time point T4, library prep Plate_16


title: whole blood, subject a2f4eb0b, sample time point T1, library prep Plate_8


title: whole blood, subject a2f4eb0b, sample time point T2, library prep Plate_4


title: whole blood, subject a103a05a, sample time point T13, library prep Plate_16


title: whole blood, subject a2ee1a84, sample time point T1, library prep Plate_11


title: whole blood, subject a103a05a, sample time point T12, library prep Plate_18


title: whole blood, subject a472cc4b, sample time point T8, library prep Plate_8


title: whole blood, subject a077aab0, sample time point T1, library prep Plate_14


title: whole blood, subject a077aab0, sample time point T2, library prep Plate_10


title: whole blood, subject a103a05a, sample time point T4, library prep Plate_6


title: whole blood, subject a46725b1, sample time point T4, library prep Plate_9


title: whole blood, subject a03b4104, sample time point T2, library prep Plate_8


title: whole blood, subject a03b4104, sample time point T4, library prep Plate_18


title: whole blood, subject a03b4104, sample time point T1, library prep Plate_13


title: whole blood, subject 9f1bc4a8, sample time point T8, library prep Plate_8


title: whole blood, subject 9f1bc4a8, sample time point T12, library prep Plate_12


title: whole blood, subject 9f621c0f, sample time point T4, library prep Plate_16


title: whole blood, subject 9f621c0f, sample time point T1, library prep Plate_12


title: whole blood, subject 9f1bc4a8, sample time point T4, library prep Plate_12


title: whole blood, subject 9f1bc4a8, sample time point T1, library prep Plate_16


title: whole blood, subject 9de59036, sample time point T0, library prep Plate_19


title: whole blood, subject 9e2d5ea6, sample time point T2, library prep Plate_2


title: whole blood, subject 9e2d5ea6, sample time point T1, library prep Plate_2


title: whole blood, subject 9dc56b1c, sample time point T2, library prep Plate_2


title: whole blood, subject 9de59036, sample time point T4, library prep Plate_8


title: whole blood, subject 9dc56b1c, sample time point T1, library prep Plate_10


title: whole blood, subject 9de59036, sample time point T1, library prep Plate_10


title: whole blood, subject 9d0c1107, sample time point T4, library prep Plate_13


title: whole blood, subject 9d0c1107, sample time point T1, library prep Plate_7


title: whole blood, subject 9ca46218, sample time point T1, library prep Plate_11


title: whole blood, subject 9ca46218, sample time point T2, library prep Plate_9


title: whole blood, subject 9cdb94d1, sample time point T4, library prep Plate_17


title: whole blood, subject 9cdb94d1, sample time point T1, library prep Plate_8


title: whole blood, subject 9c3d28d3, sample time point T1, library prep Plate_17


title: whole blood, subject 9c5c5cb0, sample time point T1, library prep Plate_13


title: whole blood, subject 9d0c1107, sample time point T2, library prep Plate_3


title: whole blood, subject 9b9f2c91, sample time point T1, library prep Plate_3


title: whole blood, subject 9b77114a, sample time point T1, library prep Plate_9


title: whole blood, subject 9b77114a, sample time point T2, library prep Plate_4


title: whole blood, subject 9f1bc4a8, sample time point T2, library prep Plate_9


title: whole blood, subject 9ab573b1, sample time point T8, library prep Plate_6


title: whole blood, subject 9ab573b1, sample time point T4, library prep Plate_8


title: whole blood, subject 9b9f2c91, sample time point T2, library prep Plate_8


title: whole blood, subject 9a6c775c, sample time point T1, library prep Plate_16


title: whole blood, subject 9ab573b1, sample time point T1, library prep Plate_4


title: whole blood, subject 9a170b5d, sample time point T4, library prep Plate_13


title: whole blood, subject 99eb6b42, sample time point T2, library prep Plate_2


title: whole blood, subject 99de72fc, sample time point T0, library prep Plate_2


title: whole blood, subject 998caea5, sample time point T8, library prep Plate_11


title: whole blood, subject 998caea5, sample time point T4B, library prep Plate_3


title: whole blood, subject 998caea5, sample time point T4A, library prep Plate_8


title: whole blood, subject 998caea5, sample time point T1, library prep Plate_8


title: whole blood, subject 998caea5, sample time point T2, library prep Plate_13


title: whole blood, subject 9ca46218, sample time point T4, library prep Plate_3


title: whole blood, subject 99159ba5, sample time point T4, library prep Plate_3


title: whole blood, subject 99159ba5, sample time point T2, library prep Plate_8


title: whole blood, subject 99159ba5, sample time point T1, library prep Plate_11


title: whole blood, subject 990a7cba, sample time point T2, library prep Plate_13


title: whole blood, subject 990a7cba, sample time point T4, library prep Plate_10


title: whole blood, subject 990a7cba, sample time point T8, library prep Plate_17


title: whole blood, subject 98c815a3, sample time point T1, library prep Plate_14


title: whole blood, subject 990a7cba, sample time point T1, library prep Plate_18


title: whole blood, subject 98c815a3, sample time point T0, library prep Plate_8


title: whole blood, subject 972d9455, sample time point T4, library prep Plate_3


title: whole blood, subject 99eb6b42, sample time point T4, library prep Plate_4


title: whole blood, subject 972d9455, sample time point T12, library prep Plate_15


title: whole blood, subject 977a55ec, sample time point T1, library prep Plate_12


title: whole blood, subject 972d9455, sample time point T8, library prep Plate_5


title: whole blood, subject 96f8e6ac, sample time point T1, library prep Plate_6


title: whole blood, subject 97eda4d9, sample time point T1, library prep Plate_15


title: whole blood, subject 998caea5, sample time point T12, library prep Plate_11


title: whole blood, subject 967e6186, sample time point T2, library prep Plate_3


title: whole blood, subject 96f8e6ac, sample time point T12, library prep Plate_5


title: whole blood, subject 961c984b, sample time point T13, library prep Plate_15


title: whole blood, subject 961c984b, sample time point T2, library prep Plate_10


title: whole blood, subject 961c984b, sample time point T12, library prep Plate_15


title: whole blood, subject 961c984b, sample time point T8, library prep Plate_8


title: whole blood, subject 961c984b, sample time point T4, library prep Plate_3


title: whole blood, subject 98c815a3, sample time point T2, library prep Plate_6


title: whole blood, subject 9615aa1c, sample time point T2, library prep Plate_12


title: whole blood, subject 948d9b6d, sample time point T4, library prep Plate_10


title: whole blood, subject 961c984b, sample time point T1, library prep Plate_13


title: whole blood, subject 9615aa1c, sample time point T1, library prep Plate_5


title: whole blood, subject 948d9b6d, sample time point T2, library prep Plate_11


title: whole blood, subject 948d9b6d, sample time point T8, library prep Plate_16


title: whole blood, subject 96073ac2, sample time point T1, library prep Plate_19


title: whole blood, subject 972d9455, sample time point T2, library prep Plate_4


title: whole blood, subject 946ec1e0, sample time point T4, library prep Plate_14


title: whole blood, subject 946ec1e0, sample time point T2, library prep Plate_19


title: whole blood, subject 948d9b6d, sample time point T1, library prep Plate_12


title: whole blood, subject 946ec1e0, sample time point T8, library prep Plate_18


title: whole blood, subject 945ddd9c, sample time point T4, library prep Plate_11


title: whole blood, subject 945ddd9c, sample time point T8, library prep Plate_15


title: whole blood, subject 945ddd9c, sample time point T2, library prep Plate_8


title: whole blood, subject 945ddd9c, sample time point T1, library prep Plate_12


title: whole blood, subject 93e555b0, sample time point T1, library prep Plate_9


title: whole blood, subject 93e555b0, sample time point T2, library prep Plate_6


title: whole blood, subject 93e555b0, sample time point T8, library prep Plate_2


title: whole blood, subject 93e555b0, sample time point T4, library prep Plate_4


title: whole blood, subject 92c9144a, sample time point T1, library prep Plate_15


title: whole blood, subject 92c9144a, sample time point T2, library prep Plate_7


title: whole blood, subject 9279c5a5, sample time point T2, library prep Plate_13


title: whole blood, subject 9246fa51, sample time point T1, library prep Plate_10


title: whole blood, subject 91245297, sample time point T13, library prep Plate_15


title: whole blood, subject 9203a023, sample time point T4, library prep Plate_19


title: whole blood, subject 91245297, sample time point T8, library prep Plate_4


title: whole blood, subject 91245297, sample time point T12, library prep Plate_15


title: whole blood, subject 91245297, sample time point T2, library prep Plate_3


title: whole blood, subject 946ec1e0, sample time point T1, library prep Plate_16


title: whole blood, subject 900d8f3a, sample time point T13, library prep Plate_17


title: whole blood, subject 901703c1, sample time point T1, library prep Plate_12


title: whole blood, subject 900d8f3a, sample time point T12, library prep Plate_19


title: whole blood, subject 900d8f3a, sample time point T4, library prep Plate_13


title: whole blood, subject 91245297, sample time point T4, library prep Plate_12


title: whole blood, subject 946a8d61, sample time point T1, library prep Plate_15


title: whole blood, subject 900d8f3a, sample time point T2, library prep Plate_15


title: whole blood, subject 90e38c54, sample time point T1, library prep Plate_18


title: whole blood, subject 900d8f3a, sample time point T1, library prep Plate_12


title: whole blood, subject 91245297, sample time point T1, library prep Plate_2


title: whole blood, subject 900d8f3a, sample time point T8, library prep Plate_16


title: whole blood, subject 8fa95a8d, sample time point T4, library prep Plate_10


title: whole blood, subject 8ef6c1bc, sample time point T2, library prep Plate_16


title: whole blood, subject 9246fa51, sample time point T2, library prep Plate_13


title: whole blood, subject 8ef6c1bc, sample time point T4, library prep Plate_16


title: whole blood, subject 8ef6c1bc, sample time point T8, library prep Plate_18


title: whole blood, subject 8ef6c1bc, sample time point T1, library prep Plate_17


title: whole blood, subject 8e87fba2, sample time point T1, library prep Plate_4


title: whole blood, subject 8e87fba2, sample time point T2, library prep Plate_12


title: whole blood, subject 8df7db09, sample time point T1, library prep Plate_14


title: whole blood, subject 8e87fba2, sample time point T4, library prep Plate_6


title: whole blood, subject 8d91c4be, sample time point T12, library prep Plate_9


title: whole blood, subject 8d91c4be, sample time point T8, library prep Plate_4


title: whole blood, subject 8d91c4be, sample time point T4, library prep Plate_7


title: whole blood, subject 8d91c4be, sample time point T2, library prep Plate_9


title: whole blood, subject 8c849dfb, sample time point T12, library prep Plate_9


title: whole blood, subject 8d91c4be, sample time point T1, library prep Plate_6


title: whole blood, subject 8c849dfb, sample time point T8, library prep Plate_6


title: whole blood, subject 8c849dfb, sample time point T1, library prep Plate_2


title: whole blood, subject 8c849dfb, sample time point T2, library prep Plate_13


title: whole blood, subject 8c7a6694, sample time point T4, library prep Plate_13


title: whole blood, subject 8a75ea1f, sample time point T1, library prep Plate_7


title: whole blood, subject 8c7a6694, sample time point T1, library prep Plate_3


title: whole blood, subject 8a75ea1f, sample time point T8, library prep Plate_11


title: whole blood, subject 8a75ea1f, sample time point T12, library prep Plate_9


title: whole blood, subject 8c7a6694, sample time point T0, library prep Plate_5


title: whole blood, subject 8a75ea1f, sample time point T0, library prep Plate_12


title: whole blood, subject 89e8a858, sample time point T4, library prep Plate_4


title: whole blood, subject 89e8a858, sample time point T2, library prep Plate_13


title: whole blood, subject 89e8a858, sample time point T1, library prep Plate_7


title: whole blood, subject 88c174e8, sample time point T1, library prep Plate_9


title: whole blood, subject 88924a03, sample time point T8, library prep Plate_15


title: whole blood, subject 88924a03, sample time point T12, library prep Plate_17


title: whole blood, subject 88924a03, sample time point T4, library prep Plate_17


title: whole blood, subject 88e05bde, sample time point T1, library prep Plate_5


title: whole blood, subject 887f501e, sample time point T8, library prep Plate_9


title: whole blood, subject 887f501e, sample time point T4, library prep Plate_14


title: whole blood, subject 887f501e, sample time point T2, library prep Plate_4


title: whole blood, subject 87f372cb, sample time point T2, library prep Plate_19


title: whole blood, subject 87f372cb, sample time point T0, library prep Plate_4


title: whole blood, subject 87906852, sample time point T1, library prep Plate_4


title: whole blood, subject 8c849dfb, sample time point T13, library prep Plate_14


title: whole blood, subject 86526fce, sample time point T12, library prep Plate_19


title: whole blood, subject 8557d073, sample time point T8, library prep Plate_5


title: whole blood, subject 84739e82, sample time point T12, library prep Plate_15


title: whole blood, subject 8557d073, sample time point T1, library prep Plate_9


title: whole blood, subject 8557d073, sample time point T4, library prep Plate_3


title: whole blood, subject 84739e82, sample time point T13, library prep Plate_18


title: whole blood, subject 857f0148, sample time point T1, library prep Plate_8


title: whole blood, subject 85d4973a, sample time point T1, library prep Plate_6


title: whole blood, subject 86526fce, sample time point T2, library prep Plate_3


title: whole blood, subject 84739e82, sample time point T4, library prep Plate_19


title: whole blood, subject 857f0148, sample time point T4, library prep Plate_16


title: whole blood, subject 857f0148, sample time point T8, library prep Plate_6


title: whole blood, subject 8b48500b, sample time point T1, library prep Plate_3


title: whole blood, subject 84739e82, sample time point T1, library prep Plate_14


title: whole blood, subject 84739e82, sample time point T2, library prep Plate_13


title: whole blood, subject 84739e82, sample time point T8, library prep Plate_15


title: whole blood, subject 83df1b39, sample time point T2, library prep Plate_10


title: whole blood, subject 835b62b9, sample time point T2, library prep Plate_7


title: whole blood, subject 835b62b9, sample time point T4, library prep Plate_8


title: whole blood, subject 88924a03, sample time point T1, library prep Plate_7


title: whole blood, subject 83df1b39, sample time point T1, library prep Plate_5


title: whole blood, subject 80c90ec1, sample time point T1, library prep Plate_18


title: whole blood, subject 83df1b39, sample time point T4, library prep Plate_17


title: whole blood, subject 83df1b39, sample time point T12, library prep Plate_17


title: whole blood, subject 835b62b9, sample time point T1, library prep Plate_13


title: whole blood, subject 80bfcac2, sample time point T13, library prep Plate_19


title: whole blood, subject 83df1b39, sample time point T8, library prep Plate_10


title: whole blood, subject 824c6f4f, sample time point T4, library prep Plate_13


title: whole blood, subject 809e4ed7, sample time point T1, library prep Plate_3


title: whole blood, subject 808fce87, sample time point T2, library prep Plate_10


title: whole blood, subject 809e4ed7, sample time point T1, library prep Plate_15


title: whole blood, subject 809e4ed7, sample time point T1, library prep Plate_5


title: whole blood, subject 8059c147, sample time point T1, library prep Plate_15


title: whole blood, subject 809e4ed7, sample time point T1, library prep Plate_17


title: whole blood, subject 7ff326e0, sample time point T8, library prep Plate_18


title: whole blood, subject 808fce87, sample time point T1, library prep Plate_13


title: whole blood, subject 7ff326e0, sample time point T12, library prep Plate_16


title: whole blood, subject 80bfcac2, sample time point T12, library prep Plate_2


title: whole blood, subject 809e4ed7, sample time point T1, library prep Plate_7


title: whole blood, subject 803c6747, sample time point T4, library prep Plate_4


title: whole blood, subject 808fce87, sample time point T4, library prep Plate_13


title: whole blood, subject 808fce87, sample time point T8, library prep Plate_16


title: whole blood, subject 80bfcac2, sample time point T8, library prep Plate_4


title: whole blood, subject 809e4ed7, sample time point T1, library prep Plate_11


title: whole blood, subject 809e4ed7, sample time point T1, library prep Plate_13


title: whole blood, subject 809e4ed7, sample time point T1, library prep Plate_9


title: whole blood, subject 809e4ed7, sample time point T1, library prep Plate_19


title: whole blood, subject 7ff326e0, sample time point T4, library prep Plate_14


title: whole blood, subject 7ff326e0, sample time point T13, library prep Plate_14


title: whole blood, subject 7ef61ea8, sample time point T4, library prep Plate_15


title: whole blood, subject 7ff326e0, sample time point T2, library prep Plate_17


title: whole blood, subject 7ef61ea8, sample time point T1, library prep Plate_14


title: whole blood, subject 7e1cecbd, sample time point T2, library prep Plate_12


title: whole blood, subject 7d976da9, sample time point T8, library prep Plate_2


title: whole blood, subject 8059c147, sample time point T2, library prep Plate_19


title: whole blood, subject 7f657e7b, sample time point T1, library prep Plate_5


title: whole blood, subject 7ff326e0, sample time point T1, library prep Plate_8


title: whole blood, subject 7ef61ea8, sample time point T2, library prep Plate_18


title: whole blood, subject 7ca6fb9f, sample time point T1, library prep Plate_2


title: whole blood, subject 7af92afb, sample time point T4, library prep Plate_7


title: whole blood, subject 7cf00ea9, sample time point T13, library prep Plate_13


title: whole blood, subject 7cf00ea9, sample time point T12, library prep Plate_4


title: whole blood, subject 79ee3c9d, sample time point T4, library prep Plate_6


title: whole blood, subject 7cf00ea9, sample time point T1, library prep Plate_5


title: whole blood, subject 7b86ffe2, sample time point T8, library prep Plate_14


title: whole blood, subject 7b86ffe2, sample time point T1, library prep Plate_9


title: whole blood, subject 7d976da9, sample time point T4, library prep Plate_7


title: whole blood, subject 7a602d10, sample time point T4, library prep Plate_19


title: whole blood, subject 7a602d10, sample time point T1, library prep Plate_13


title: whole blood, subject 7b86ffe2, sample time point T2, library prep Plate_19


title: whole blood, subject 7b86ffe2, sample time point T12, library prep Plate_18


title: whole blood, subject 78e0c551, sample time point T12, library prep Plate_16


title: whole blood, subject 78e0c551, sample time point T8, library prep Plate_9


title: whole blood, subject 79c38c5e, sample time point T8, library prep Plate_4


title: whole blood, subject 79ee3c9d, sample time point T1, library prep Plate_13


title: whole blood, subject 77e6b633, sample time point T4, library prep Plate_7


title: whole blood, subject 79c38c5e, sample time point T1, library prep Plate_12


title: whole blood, subject 7b86ffe2, sample time point T4, library prep Plate_15


title: whole blood, subject 78e0c551, sample time point T2, library prep Plate_12


title: whole blood, subject 78e0c551, sample time point T4, library prep Plate_4


title: whole blood, subject 78e0c551, sample time point T1, library prep Plate_7


title: whole blood, subject 77e6b633, sample time point T2, library prep Plate_7


title: whole blood, subject 77e6b633, sample time point T1, library prep Plate_16


title: whole blood, subject 7d976da9, sample time point T1, library prep Plate_15


title: whole blood, subject 79ee3c9d, sample time point T2, library prep Plate_13


title: whole blood, subject 772dda97, sample time point T4, library prep Plate_2


title: whole blood, subject 79c38c5e, sample time point T2, library prep Plate_19


title: whole blood, subject 772dda97, sample time point T1, library prep Plate_12


title: whole blood, subject 76a9f3fc, sample time point T1, library prep Plate_9


title: whole blood, subject 7535c499, sample time point T1, library prep Plate_12


title: whole blood, subject 772d4e98, sample time point T1, library prep Plate_11


title: whole blood, subject 74d9ca69, sample time point T12, library prep Plate_4


title: whole blood, subject 74d9ca69, sample time point T1, library prep Plate_13


title: whole blood, subject 7378f344, sample time point T8, library prep Plate_3


title: whole blood, subject 70c4857c, sample time point T4, library prep Plate_16


title: whole blood, subject 72b71371, sample time point T1A, library prep Plate_7


title: whole blood, subject 735b4b95, sample time point T4, library prep Plate_11


title: whole blood, subject 7085fdb1, sample time point T1, library prep Plate_17


title: whole blood, subject 74d9ca69, sample time point T13, library prep Plate_13


title: whole blood, subject 7378f344, sample time point T2, library prep Plate_5


title: whole blood, subject 70c4857c, sample time point T1, library prep Plate_11


title: whole blood, subject 7085fdb1, sample time point T1, library prep Plate_11


title: whole blood, subject 735b4b95, sample time point T1, library prep Plate_9


title: whole blood, subject 772dda97, sample time point T2, library prep Plate_5


title: whole blood, subject 7213a9b9, sample time point T1, library prep Plate_2


title: whole blood, subject 7213a9b9, sample time point T4, library prep Plate_8


title: whole blood, subject 7085fdb1, sample time point T1, library prep Plate_19


title: whole blood, subject 7085fdb1, sample time point T1, library prep Plate_13


title: whole blood, subject 7085fdb1, sample time point T1, library prep Plate_9


title: whole blood, subject 7085fdb1, sample time point T1, library prep Plate_7


title: whole blood, subject 735b4b95, sample time point T2, library prep Plate_15


title: whole blood, subject 701db55a, sample time point T8, library prep Plate_2


title: whole blood, subject 7085fdb1, sample time point T1, library prep Plate_3


title: whole blood, subject 7085fdb1, sample time point T1, library prep Plate_5


title: whole blood, subject 701db55a, sample time point T1, library prep Plate_2


title: whole blood, subject 72b71371, sample time point T1B, library prep Plate_18


title: whole blood, subject 7085fdb1, sample time point T1, library prep Plate_15


title: whole blood, subject 6f7d59dd, sample time point T12, library prep Plate_7


title: whole blood, subject 6f7d59dd, sample time point T8, library prep Plate_9


title: whole blood, subject 6f7d59dd, sample time point T4, library prep Plate_11


title: whole blood, subject 6f7d59dd, sample time point T2, library prep Plate_4


title: whole blood, subject 6e29f9d9, sample time point T8, library prep Plate_10


title: whole blood, subject 6dba65a2, sample time point T8, library prep Plate_9


title: whole blood, subject 6e29f9d9, sample time point T1, library prep Plate_14


title: K562_PDSbtn_3hr_10xPDS_B1_t5

city: Cambridge

first: Larry

last: Melidis

state: Not US or Canada

address: Robinson Way

department: CRUK-CI

country: United Kingdom

institute: University of Cambridge

description: K562 cells PDS-btn Chem-map non-fixed cells

library_source: genomic

data_processing:
fq files were trimed with cutadapt version 1.18 to remove adaptors and bases with qualiti lower than 20 (-q 20) mapped with bwa-mem 0.7.17-r2188 to hg38 genome, and only the reads within the whitelist have been retained.
bw files were created using bamCoverage with counts per milion normalisation (CPM) and extending paired end reads
Assembly: hg38
Supplementary files format and content: bigWig

growth_protocol: Mycoplasma-free human chronic myelogenous leukemia K562 cells (RRID:CVCL 0004) were purchased from ATCC and cultured in RPMI 1640 (Gibco, 21875034) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Gibco, A3840401). Human bone osteosarcoma epithelial U2OS cells (RRID:CVCL_0042) derived from a moderately differentiated sarcoma were obtained from ATCC and cultured in DMEM (Gibco, 41966029) supplemented with 10% FBS. Both cell lines were grown in accordance with ENCODE cell culture protocols and tested for mycoplasma contamination and identity confirmed by STR typing.

extract_protocol:
For Chem-map experiment, U2OS cells were detached using accutase (Stem Cell Technologies, 07920) and quenched using complete culture media. U2OS and K562 cells were collected by centrifugation and fixed in 0.1% formaldehyde (Thermo Fisher Scientific, 28906) in PBS for 2 min at room temperature, followed by quenching with glycine (Sigma-Aldrich, 50046) to a final concentration of 75 mM. Fixed cells were collected by centrifugation at 600x g for 4 min, followed by resuspension in cold wash buffer (20 mM HEPES, pH 7.5, 150 mM NaCl and 0.5 mM spermidine (Sigma-Aldrich, S0266)) in nuclease-free water supplemented with a Complete Protease Inhibitor, EDTA-free (Sigma-Aldrich, 11873580001). [Note: For Chem-map of G4 ligands, NaCl was replaced by equivalent concentrations of KCl in all buffers to maintain G4 stability]. For 5 samples, 50 μL (for JQ1-btn) or 75 µL (for G4 ligands and biotinylated doxorubicin) concanavalin A beads (Bangs Labs, BP531) were washed twice in 1 mL binding buffer (20 mM HEPES, pH 7.5, 10 mM KCl, 1 mM CaCl2, 1 mM MnCl2 in nuclease-free water) and resuspended in 75 µL binding buffer. 100 µL of cell suspension were incubated with 10 µL pre-washed concanavalin A beads at 25 °C for 10 min at 600 rpm. Beads-bound cells were gently washed twice with wash buffer, before resuspending in 100 µL probe-1st Ab pre-complex (small molecule concentration is 400 nM with a ratio of 1.2:1 to anti-biotin (D5A7) Rabbit mAb (Cell Signaling Technology, 5597)) in antibody buffer (2 mM EDTA, 0.1% BSA (Sigma-Aldrich, A8577), 0.05% digitonin (EMD Millipore, 300410) in wash buffer) and incubating at 4 °C overnight at 600 rpm. [Note: For Dox-btn1, final Dox-btn1 concentration is diluted to 0.2 µM]. Cells were washed 3x with 500 µL Dig-wash buffer (0.05% digitonin in wash buffer) and resuspended in 100 µL 2nd Ab-Tn5 transposome complex (2nd Ab (Antibodies-Online, ABIN101961, ~ 8 µM) ratio is 1:100; 2nd Ab and pA-Tn5 at a ratio of 2:1) in Dig-300 buffer (20 mM HEPES pH 7.5, 300 mM NaCl, 0.5 mM spermidine, 0.01% digitonin in nuclease-free water supplemented with Complete Protease Inhibitor, EDTA-free) and incubated at 25 °C for 1 hr at 600 rpm. Cells were then washed 3x in 500 µL Dig-300 buffer before incubation in 300 µL tagmentation buffer (10 mM MgCl2 in Dig-300 buffer) at 37 °C for 1 hr at 600 rpm. After tagmentation, cells were washed twice with 500 µL TAPS wash buffer (10 mM [tris(hydroxymethyl) methylamino] propanesulfonic acid (TAPS, Alfa Aesar, J63268.AE), 0.2 mM EDTA in nuclease-free water). 150 µL of extraction buffer (0.5 mg/mL proteinase K (Thermo Fisher Scientific, EO0491), 0.5% SDS (Sigma-Aldrich, L4509) in 10 mM Tris-HCl, pH8.0) were added, vortexed and incubated at 55 °C for 1 hr at 800 rpm. Next, 150 µL phenol-chloroform-isoamyl alcohol (Invitrogen, 15593049) was added and mixed. The mixture was transferred to MaXtract High Density phase-lock tubes (QIAGEN, 129046) and centrifuged at room temperature for 3 min at 16,000x g. 150 µL chloroform were added to the top aqueous phase, mixed by inverting the phase-lock tubes for 10 times and centrifuged at room temperature for 3 min at 16,000x g. The top aqueous layer was transferred to a 1.5 mL DNA Lo-bind tube (Eppendorf, 022431021). 6 µL 5 M NaCl and 375 µL cold ethanol were added, mixed and incubated at -20 °C overnight. Samples were centrifuged at 4 °C for 30 min at 21,130x g. The supernatant was carefully poured off and the DNA pellet rinsed with 1 mL cold 100% ethanol followed by centrifugation at 4 °C for 2 min at 21,130x g. After pouring off the wash and draining the residual liquid with paper towel, the pellet was left to air-dry. Finally, the pellet was resuspended in 25 µL elution buffer 1 (10 mM Tris-HCl, pH8, 1 mM EDTA, 1/400 RNAse A (Thermo Fisher Scientific, EN0531) in nuclease-free water) by vortexing and incubating at 37 °C for 10 min at 800 rpm.
For BRD4 CUT&Tag experiment, U2OS cells were detached using accutase (Stem Cell Technologies, 07920) and quenched using complete culture media. U2OS and K562 cells were collected by centrifugation and fixed in 0.1% formaldehyde (Thermo Fisher Scientific, 28906) in PBS for 2 min at room temperature, followed by quenching with glycine (Sigma-Aldrich, 50046) to a final concentration of 75 mM. Fixed cells were collected by centrifugation at 600x g for 4 min, followed by resuspension in cold wash buffer (20 mM HEPES, pH 7.5, 150 mM NaCl and 0.5 mM spermidine (Sigma-Aldrich, S0266)) in nuclease-free water supplemented with a Complete Protease Inhibitor, EDTA-free (Sigma-Aldrich, 11873580001). ConA beads (Bangs Labs, BP531) per sample were washed twice in 1 mL binding buffer (20 mM HEPES, pH 7.5, 10 mM KCl, 1 mM CaCl2, 1 mM MnCl2 in nuclease-free water) and resuspended in 75 µL binding buffer. 100 µL of cell suspension were incubated with 10 µL pre-washed concanavalin A beads at 25 °C for 10 min at 600 rpm. Beads-bound cells were gently washed twice with wash buffer, before resuspending in 50 µL anti-BRD4 (E2A7X) rabbit antibody (Cell Signalling Technology, 13440) in antibody buffer (2 mM EDTA, 0.1% BSA (Sigma-Aldrich, A8577), 0.05% digitonin (EMD Millipore, 300410) in wash buffer) at a ratio of 1:50 and incubating at 4 °C overnight at 600 rpm. Cells were washed 3x with 500 µL Dig-wash buffer (0.05% digitonin in wash buffer) and resuspended in 50 µL Guinea Pig anti-rabbit antibody (Antibodies-Online, ABIN101961) in Dig-wash buffer at a ratio of 1:100. Diluted pA-Tn5 adapter complex (1:250 ratio) was then added in Dig-300 buffer followed by the tagmentation reaction in tagmentation buffer (20 mM HEPES pH 7.5, 300 mM NaCl, 0.5 mM spermidine, 0.01% digitonin in nuclease-free water supplemented with Complete Protease Inhibitor, EDTA-free) and incubated at 25 °C for 1 hr at 600 rpm. Cells were then washed 3x in 500 µL Dig-300 buffer before incubation in 300 µL tagmentation buffer (10 mM MgCl2 in Dig-300 buffer) at 37 °C for 1 hr at 600 rpm. After tagmentation, cells were washed twice with 500 µL TAPS wash buffer (10 mM [tris(hydroxymethyl) methylamino] propanesulfonic acid (TAPS, Alfa Aesar, J63268.AE), 0.2 mM EDTA in nuclease-free water). 100 µL of extraction buffer (0.5 mg/mL proteinase K (Thermo Fisher Scientific, EO0491), 0.5% SDS (Sigma-Aldrich, L4509) in 10 mM Tris-HCl, pH8.0) were added, vortexed and incubated at 55 °C for 1 hr at 800 rpm. Next, 100 µL phenol-chloroform-isoamyl alcohol (Invitrogen, 15593049) was added and mixed. The mixture was transferred to MaXtract High Density phase-lock tubes (QIAGEN, 129046) and centrifuged at room temperature for 3 min at 16,000x g. 100 µL chloroform were added to the top aqueous phase, mixed by inverting the phase-lock tubes for 10 times and centrifuged at room temperature for 3 min at 16,000x g. The top aqueous layer was transferred to a 1.5 mL DNA Lo-bind tube (Eppendorf, 022431021). 4 µL 5 M NaCl and 250 µL cold ethanol were added, mixed and incubated at -20 °C overnight. Samples were centrifuged at 4 °C for 30 min at 21,130x g. The supernatant was carefully poured off and the DNA pellet rinsed with 1 mL cold 100% ethanol followed by centrifugation at 4 °C for 2 min at 21,130x g. After pouring off the wash and draining the residual liquid with paper towel, the pellet was left to air-dry. Finally, the pellet was resuspended in 25 µL elution buffer 1 (10 mM Tris-HCl, pH8, 1 mM EDTA, 1/400 RNAse A (Thermo Fisher Scientific, EN0531) in nuclease-free water) by vortexing and incubating at 37 °C for 10 min at 800 rpm.
In a 0.2 mL PCR tube, 25 µL NEBNext® Ultra™ II Q5 2x PCR master mix (NEB, M0544), 2 µL of 10 µM uniquely barcoded v2 Ad1.x primer, 2 µL of 10 µM uniquely barcoded v2 Ad2.x primer and 21 µL tagmented DNA were added and subjected to PCR (72 °C for 5 min, 98 °C for 30 s, followed by 10 cycles of 98 °C for 10 s and 63 °C for 10 s, and one cycle of 72 °C for 1 min). Libraries were purified using 1.3x ratio (65 µL) Ampure XP beads (Beckman Coulter, A63882). After 10 min incubation at room temperature, bead-bound DNA was washed twice with 80% ethanol and libraries were eluted with 25 µL 10 mM Tris-HCl for 5 min at room temperature. [Note: PCR program condition for Dox-btn1 is 72 °C for 5 min, 98 °C for 2 min, followed by 10 cycles of 98 °C for 10 s and 63 °C for 10 s, and one cycle of 72 °C for 1 min, to maximally disrupt doxorubicin-DNA interaction]

value: erythroleukemia type

tag: chip antibody

value: PDS-btn


title: K562_PDSbtn_3hr_10xPDS_B1_t3


title: K562_PDSbtn_3hr_10xPDS_B1_t2


title: K562_PDSbtn_3hr_10xPDS_B1_t4


title: THP1 Mutant, TFEB

city: Bethesda

first: Jose

last: Martina

state: Maryland

address: 50 South Dr., Building 50, Room 3533

department: NHLBI, CDBC

institute: NIH

data_processing:
Basecalls performed using Illumina bcl2fastq2 version 2.20
ChIP-seq reads were aligned to the human GRCh38/hg38 genome assembly using Burrows-Wheeler Aligner algorithm with default settings.
Data were filtered as follows: Duplicate reads were removed, and only uniquely mapped reads (mapping quality >= 25) were used for further analysis. Alignments were extended in silico at their 3’-ends to a length of 200 bp, which is the average genomic fragment length in the size-selected library and assigned to 32-nt bins along the genome. The resulting histograms (genomic “signal maps”) were stored in bigWig files.
Peak locations were determined using the MACS algorithm (v2.1.0) with a cutoff of p-value = 1e-7 [2]. Peaks that were on the ENCODE blacklist of known false ChIP-Seq peaks were removed.
Assembly: hg38
Supplementary files format and content: bigWIG files were generated using wigToBigWig version 4 software

growth_protocol: THP1 (TFEB-WT or CRISPR knock-in TFEB-S401A) cells  were grown in RPMI supplemented with GlutaMAX (Gibco, 61870036), 10% fetal bovine serum (Invitrogen, 21041-025) and 100 U/ml penicillin and 100 μg/ml streptomycin (Gibco, 2114).

extract_protocol:
Chromatin was isolated from cells by adding lysis buffer (1% SDS, 10 mM EDTA and 50mM Tris-HCl, pH 8.1 containing protease inhibitors), followed by disruption with a Dounce homogenizer. Lysates were sonicated and the DNA sheared to an average length of 300-500 bp with Active Motif’s EpiShear probe sonicator (cat# 53051). Genomic DNA (Input) was prepared by treating aliquots of chromatin with RNase, proteinase K and heat for de-crosslinking, followed by SPRI beads clean up (Beckman Coulter) and quantitation by Clariostar (BMG Labtech). Genomic DNA regions of interest were isolated using monoclonal antibody against Flag.
Illumina sequencing libraries were prepared from the ChIP and Input DNAs by the standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation. Steps were performed on an automated system (Apollo 342, Wafergen Biosystems/Takara) following the manufacturer's protocol. After a final PCR amplification step, the resulting DNA libraries were quantified and sequenced on Illumina’s NextSeq 500 (75 nt reads, single end) following the manufacturer's protocols.

treatment_protocol: Cells were incubated with 50 ng/ml PMA for 12h.

value: Monocytic

tag: disease state

value: Leukemia

value: Rabbit anti-TFEB ( Cell Signaling Technology, Cat# 37785)

value: Treated with 50 ng/ml PMA for 12h

value: CRISPR knock-in TFEB-S401A


title: K562_PDSbtn_unfixed_B1_t5


title: Biotin_unfixed_B1_t1

description: K562 biotin only control Chem-map non-fixed cells

value: Biotin


title: whole blood, subject 6dba65a2, sample time point T2, library prep Plate_5

tag: tissue

value: whole blood

value: Female

value: Positive


title: THP1 WT, TFEB

tag: genotype

value: TFEB-WT


title: whole blood, subject 6dba65a2, sample time point T1, library prep Plate_13

tag: subject id

tag: blood sample_id


title: K562_PDSbtn_3hr_10xPDS_B1_t1

description: K562 cells PDS-btn Chem-map competition


title: K562_PDSbtn_unfixed_B1_t4


title: whole blood, subject 6d81a52b, sample time point T1, library prep Plate_14

tag: library prep_plate

value: Male

tag: sampling time_point_label


title: whole blood, subject 6d81a52b, sample time point T2, library prep Plate_8

tag: days since_first_sample

tag: patient classification_at_first_sample


title: K562_PDSbtn_unfixed_B1_t3

tag: cell line


title: whole blood, subject 6f7d59dd, sample time point T13, library prep Plate_2

tag: covid-19 positive


title: K562_PDSbtn_unfixed_B1_t2

tag: cell type


title: Pooled, Input

tag: treatment


title: K562_PDSbtn_unfixed_B1_t1


title: whole blood, subject 6decc33d, sample time point T1, library prep Plate_16

tag: Sex

value: Negative


title: whole blood, subject 6dba65a2, sample time point T4, library prep Plate_14


title: whole blood, subject 6ce79fdf, sample time point T1, library prep Plate_3


title: K562, pH7.2, 37C, rep2, iMotif

city: Sydney

first: Samuel

last: Ross

state: NSW

address: School of Biotechnology and Biomolecular Sciences

country: Australia

institute: The University of New South Wales

data_processing:
15 bp from 5' end of reads were trimmed using cutadapt version 3.4 as per recommendation by the manufacturer of the library prep kit
Trimmed reads were aligned to the UCSC hg38 genome using Bowtie version 2.4.2 with default settings
Sam files were sorted, filtered, and converted to Bam using Sambamba 0.6.7 and proper_pair and mapping_quality >= 30
Bam files were deduplicated using sambamba markup
Deduplicated bam files were split into plus or minus mapping read pairs, based on the mapping of the first read in each pair. Flags 99 and 147 were used to identify plus mapping read pairs, while Flags 83 and 163 were used to identify minus mapping read pairs
BigWig files were generated using deepTools 3.5.0 bamCoverage and CPM normalisation
Peaks were called using Macs2 2.2.7.1 with the following options "callpeak -f BAMPE -t  pulldown.bam -c input.bam -g hs -q 0.01 -B"
Assembly: hg38
Supplementary files format and content: bigWig

growth_protocol: K-562 cells (ATCC: CCL-243) were cultured in RPMI-1640 medium supplemented with 10% FBS and 1% Pen/Strep until they reached to 2-7 x 10^5 /mL cell density . Cells were then harvested, pelleted  and kept at - 80 celsius degree until extraction and purification of the genomic DNA.

extract_protocol:
Genomic DNA were purified using QIAGEN AllPrep DNA/RNA/protein mini kit (Cat. No.: 80004) following the manufacturer protocol.
100 ng of pulled down DNA were used for library preparation using Accel-NGS 1S Plus DNA Library kit (Swift Biosciences, Cat. No.: 10024)  and 1S Plus Dual Indexing kit (Swift Biosciences, Cat. No.: 16024) following the manufacturer protocol.
Libraries were prepared using a kit specific for single-stranded DNA and sequenced on NextSeq 500 using 2 x 75 bp High Output flowcell

value: human myelogenous leukemia cell line

value: i-Motif pulldown


title: whole blood, subject 6b587132, sample time point T4, library prep Plate_10


title: K562, rep2, Input

department: School of Biotechnology and Biomolecular Sciences

value: Input


title: whole blood, subject 6c8d28e2, sample time point T1, library prep Plate_2


title: whole blood, subject 6daa375c, sample time point T1, library prep Plate_16


title: whole blood, subject 6b587132, sample time point T2, library prep Plate_18


title: K562, pH7.2, 37C, rep1, iMotif


title: K562, rep1, Input


title: whole blood, subject 6b587132, sample time point T1, library prep Plate_6


title: whole blood, subject 6ac7cb7b, sample time point T4, library prep Plate_5


title: K562_Biotin_control_B2_t5

description: K562 biotin only control Chem-map

data_processing:
fq files were trimed with cutadapt version 1.18 to remove adaptors and bases with qualiti lower than 20 (-q 20) mapped with bwa-mem 0.7.17-r2188 to hg38 genome, and only the reads within the whitelist have been retained.
bw files were created using bamCoverage with counts per milion normalisation (CPM)
Assembly: hg38
Supplementary files format and content: bigWig
Library strategy: Chem-map

value: erythroleukemia type


title: U2OS_DMSO_Biotin_B2_t1

description: U2OS biotin only control Chem-map

value: osteosarcoma epithelial


title: K562_Biotin_control_B2_t3

description: K562 biotin only control Chem-map


title: whole blood, subject 6b1b752d, sample time point T1, library prep Plate_12


title: K562_Biotin_control_B2_t1

description: K562 biotin only control Chem-map


title: K562_Biotin_control_B2_t2

description: K562 biotin only control Chem-map


title: whole blood, subject 6ac7cb7b, sample time point T2, library prep Plate_17


title: U2OS_DMSO_Biotin_B1_t2

description: U2OS biotin only control Chem-map


title: whole blood, subject 6ac7cb7b, sample time point T8, library prep Plate_13


title: whole blood, subject 6ccf5fd9, sample time point T1, library prep Plate_12


title: K562_Biotin_control_B1_t4

description: K562 biotin only control Chem-map


title: whole blood, subject 6a0f70fd, sample time point T2, library prep Plate_15


title: U2OS_DMSO_Biotin_B1_t1

description: U2OS biotin only control Chem-map


title: K562_Biotin_control_B1_t5

description: K562 biotin only control Chem-map


title: K562_Biotin_control_B2_t4

description: K562 biotin only control Chem-map


title: whole blood, subject 6ac7cb7b, sample time point T1, library prep Plate_11


title: K562_Biotin_control_B1_t3

description: K562 biotin only control Chem-map


title: whole blood, subject 6b1b752d, sample time point T8, library prep Plate_19


title: whole blood, subject 6a0f70fd, sample time point T1, library prep Plate_14


title: K562_Biotin_control_B1_t2

description: K562 biotin only control Chem-map


title: K562_Biotin_control_B1_t1

description: K562 biotin only control Chem-map


title: U2OS_JQ1_B2_t4

description: U2OS cells JQ1 Chem-map


title: whole blood, subject 6968e8c2, sample time point T2, library prep Plate_7


title: whole blood, subject 6968e8c2, sample time point T1, library prep Plate_8


title: whole blood, subject 6968e8c2, sample time point T4, library prep Plate_3


title: whole blood, subject 6968e8c2, sample time point T8, library prep Plate_10


title: U2OS_JQ1_B2_t2

description: U2OS cells JQ1 Chem-map


title: whole blood, subject 695f8fdc, sample time point T12, library prep Plate_15


title: whole blood, subject 695f8fdc, sample time point T8, library prep Plate_19


title: U2OS_JQ1_B2_t1

description: U2OS cells JQ1 Chem-map


title: whole blood, subject 6b1b752d, sample time point T12, library prep Plate_18


title: U2OS_JQ1_B1_t5

description: U2OS cells JQ1 Chem-map