title: R12-1

data_processing:
We first identified all statistically enriched terms (can be GO/KEGG terms, canonical pathways, hall mark gene sets, etc., based on what your choice during the analysis), accumulative hypergeometric p-values and enrichment factors were calculated and used for filtering.  Remaining significant terms were then hierarchically clustered into a tree based on Kappa-statistical similarities among their gene memberships (similar to what is used in NCI DAVID site).      We then selected a subset of representative terms from this cluster and convert them into a network layout.  More specifically, each term is represented by a circle node, where its size is proportional to the number of input genes fall into that term, and its color represent its cluster identity (i.e., nodes of the same color belong to the same cluster).  Terms with a similarity score > 0.3 are linked by an edge (the thickness of the edge represents the similarity score).  The network is visualized with Cytoscape (v3.1.2) with “force-directed” layout and with edge bundled for clarity.  One term from each cluster is selected to have its term description shown as label.
Then 0.3 kappa score was applied as the threshold to cast the tree into term clusters.  The terms within each cluster are exported in the Excel spreadsheet named “Enrichment Analysis”.
We then selected a subset of representative terms from this cluster and convert them into a network layout.  More specifically, each term is represented by a circle node, where its size is proportional to the number of input genes fall into that term, and its color represent its cluster identity (i.e., nodes of the same color belong to the same cluster).  Terms with a similarity score > 0.3 are linked by an edge (the thickness of the edge represents the similarity score).  The network is visualized with Cytoscape (v3.1.2) with “force-directed” layout and with edge bundled for clarity.  One term from each cluster is selected to have its term description shown as label.
Genome_build: mm10
Supplementary_files_format_and_content: txt files incude gene data

source_name: Heart

extract_protocol:
Heart tissues were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraies were prepared for sequencing using standard illumina protocols

treatment_protocol: Mice were exposed to sham,Ischemia, I/R(0.5h, 1.0g, 1.5h, 3.0h, 6.0h, 12h)

value: ishchemia for 45min and reperfusion for 12h


title: 1_Male PN10 GF

status: Public on Jan 01 2023

type: SRA

city: Rockville

first: Julian

last: Lui

state: MD

address: 605 WATTS BRANCH PKWY, 10 Center Drive

country: USA

institute: NICHD

library_source: transcriptomic

data_processing:
RNA-Seq reads were trimmed with cutadapt (-AAGATCGGAAGAGCACACGTCTGAACTCCAGTCA  -AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT -overlap 6 -q 20 -minimum-length 25) and aligned using STAR (2 pass alignment) to mouse mm10 reference genome sequences.
Assembly: mm10

molecule: total RNA

organism: Mus musculus

source_name: Male PN10 GF

extract_protocol:
PNday10-old mice were killed (n=10 per exposure group) and the lungs excised. A 3mm×3mm tip of the left lung were snap frozen in liquid nitrogen and kept at -80°C. RNA extraction on the lung tissue was carried out utilizing magnetic beads technology, on a chemagic Prepito® (Perkin Elmer, Waltham, Massachussets), as recommended by the manufacturers. Concentration and purity were measured with a NanoDrop1000 spectrophotometer, with all samples showing an A260/280 ratio between 1.9 and 2.1. RNA integrity was analyzed using a 2100 Bioanalyzer (Agilent Technologies) with Agilent RNA 6000 Pico Kit (Agilent Technologies) as recommended by manufacturer. All samples used for RNAseq displayed RNA integrity number (RIN) above 7.
Sequencing libraries were prepared using a TruSeq Stranded mRNA Prep Kit (Illumina), without the polyA selection step. Sequencing was performed via a paired end 75 cycle on Illumina HiSeq 2500.

treatment_protocol: Pregnant BALB/c germ-free mice were kept in separate germ-free isolators until either (a) no exposure (GF), (b) exposure to either Bifidobacterium animalis ssp. Lactis (BI04) or to (c) full cecum content harvested from female SPF mice (Cecum). Subsequently, perinatally exposed offspring were used for the analyses. Lung tissue transcriptomics analysis was done at postnatal day 10 (PNday10) at first phase of lung alveolar development. RNA were extracted for bulk RNA-sequencing.

tag: cell type

value: lung

tag: genotype

value: WT

tag: treatment

value: untreated

tag: Sex

value: male

tag: time

value: 10day old


title: R6-3

city: Guangzhou

first: Haiqiong

last: Liu

state: Guangdong

address: Gongye Avenue 253

country: China

institute: Zhujiang Hospoital, Southern Medical University

tag: strain

tag: tissue

value: Heart

value: ishchemia for 45min and reperfusion for 6.0h


title: R6-2


title: R6-1


title: KO_CT1

data_processing:
data filering use fastp 0.21.0
data QC(fastqc and multiqc)
data aligment:bowtie2-2.3.5.1
Genetic quantitative : RSEM v1.3.1
diff gene Expression :DESeq2
Genome_build: ref genome:Mus_musculus gencode.vM27
Supplementary_files_format_and_content: geneExpression matrix,include all geneExpression of each sample

source_name: neonatal mouse heart cell

growth_protocol: Neonatal hearts were isolated from 1-3-day-old new-born C57BL/6J mice(WT and ATF3-KO) as previously described and cultured in DMEM (Gibco; Thermo Fisher Scientific, Inc.) with 100 U/ml penicillin–streptomycin and 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) at 37℃ with 5% CO2 for follow-up experiments.

extract_protocol:
Cells were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraies were prepared for sequencing using standard illumina protocols

treatment_protocol: Control: with no treatment; H/R: PBS was used to replace the former medium, and cells were cultured in a triple gas incubator with 95% N2 and 5% CO2 at 37 ℃ for 3 h under hypoxia. Reoxygenation was then initiated by medium change to replace PBS, and cells were placed back into incubated in a normal incubator at 37℃.Before H/R, cells were transfectied with Adv-GFP(KO_HG) or ATF3(KO_HA).

value: ATF3-KO control


title: KO_HR2

value: KO cells for H/R model


title: WT_HR2

value: wt cells for H/R model


title: WT_HR1


title: R3-1

value: ishchemia for 45min and reperfusion for 3.0h


title: R12-2


title: R12-3


title: R1.5-1

value: ishchemia for 45min and reperfusion for 1.5h


title: R1-3

value: ishchemia for 45min and reperfusion for 1h


title: R3-3


title: Control_2

city: Shanghai

first: Xinrui

last: Wang

address: 197 Ruijin Rd II

institute: Rui-Jin Hospital affiliated to Shanghai Jiao Tong University School of Medicine

data_processing:
Raw data (raw reads) were processed using Trimmomatic. The reads  containing ploy-N and the low quality reads were removed to obtain the clean reads.
The clean reads were mapped to reference genome using hisat2.
Read counts for each gene were carried out using HT-Seq using the GRCm39 refSeq refFlat GTF file.
Genes with P-value < 0.05 and foldChange >1.2 were defined as differentially expressed genes (DEGs).
Genome_build: GRCm39
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample ...
Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every sample

source_name: Testis

extract_protocol:
The total RNA of mouse testis in each group was isolated using RNAiso Plus (TakaRa, 9108) and stored at -80℃. The concentration and purity of RNA were determined using a NanoPhotometer (ThermoFisher Scientific Intertek, Cat. 3100668).
RNA purification, cDNA synthesis and RNA-Seq of each group (n = 3) were performed by Oebiotech company (Shanghai, China). RNA integrity was analyzed through the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara). The RNA integrity number ≥ 7 was subjected to the following analysis. The cDNA libraries were constructed through TruSeq Stranded mRNA LTSample Prep Kit (Illumina, San Diego, CA, USA).Shanghai

value: ICR

value: Testis

tag: age

value: 8 week

value: PBS injection


title: R3-2


title: R1.5-3


title: R1.5-2


title: R1-2


title: R1-1


title: H1

value: ishchemia for 45min


title: S2

value: sham sugery


title: BackSkin_fibroblast_rep3

city: Chicago

first: Mateusz

middle: S

last: Wietecha

state: IL

address: 801 S Paulina St

department: Oral Biology

institute: University of Illinois Chicago

data_processing:
Sequence alignment to the mouse reference genome (build GRCm38), quality control, and initial bioinformatics analyses were performed in the CLC Genomics Workbench (version 12) (Qiagen). All samples generated approximately 16 million reads, showed high (98.5-98.8%) mapping rates to the mouse reference genome, high (96.0-97.1%) mapping rates to genes, and low (0.15–0.39%) rRNA mapping rates.
Numbers of RNA transcripts were determined on the gene level and normalized to reads per kilobase of transcript, per million mapped reads (RPKM). Principal component analysis (PCA) was performed to evaluate grouping of samples. Differential expression analysis was performed across all group comparisons using the edgeR exact test as implemented in the CLC Genomics Workbench (Qiagen), and differentially expressed genes (DEGs) were determined and compared.
Genome_build: GRCm38
Supplementary_files_format_and_content: Tab-delimited table format containing gene names (annotated by HGNC-approved gene symbol and Ensembl identifier) and nomalized RPKM values for all samples.

source_name: Fibroblast

extract_protocol:
Total RNA was isolated using the RNeasy Micro Kit (Qiagen). RNA was analyzed via TapeStation (Agilent), and samples with RIN>7.0 were adjusted to 3 ng/μl.
RNA samples were subjected to the RNA sequencing protocol via poly-A enrichment, True-Seq library preparation, and single-end 100 bp sequencing on an Illumina HiSeq 2500 v3 instrument at the Functional Genomics Center Zurich (FGCZ).

treatment_protocol: Back skin and papillomas were harvested, minced with scissors, and digested with 0.25 mg/ml Liberase TL (Roche) in DMEM for 1 h at 37°C. Following digestion, the cell suspension was filtered through a 70  µm nylon strainer, centrifuged at 300g at 4°C for 10 min, and resuspended in FACS buffer. Back skin and skin cancer-associated fibroblasts were enriched by FACS sorting of CD45-negative/CD140a-positive (CD45-CD140a+) cells. CD3, CD11b and F4/80 were used as additional negative markers. Dead cells were excluded by Sytox Green staining. Cells were sorted into cold empty Eppendorf tubes with FACS buffer containing RNAase inhibitor and centrifuged for total RNA extraction.

value: Back skin

value: Fibroblast

tag: sorted cells

value: FACS-sorted CD45-CD140a+ live cells

value: 68-70 weeks


title: Tumor_fibroblast_rep1

value: Papilloma on back skin

value: 70 weeks


title: BackSkin_fibroblast_rep1


title: R0.5-1

value: Heart

value: ishchemia for 45min and reperfusion for 0.5h


title: N28_H3K4me1

city: Beijing

first: Jinxiu

last: Li

state: Beijing

address: Yuanmingyuan west road 2#

department: College of biological sciences

institute: China Agricutural University

library_source: genomic

data_processing:
Paired-end 75bp sequencing on an Illumina HiSeq platform
ChIP-seq reads were aligned to the mm10 genome assembly using novoalign-4.00.01
peaks were called using MACS2 with following settings: -B --SPMR --pvalue=1e-2 --mfold 15 100
Genome_build: mm10
Supplementary_files_format_and_content: bigwig files were generated using multirep_macs2_pipeline.pl; Scores represent a normalized pileup signal

molecule: genomic DNA

source_name: mouse ASPS tumor

extract_protocol:
Nuclei isolated from snap frozen samples. Lysates were clarified from sonicated nuclei and histone DNA complexes were isolated with antibody.
Library was prepared using NEBnext ChIP-seq libraray prep reagent set

tag: chip antibody

value: mouse ASPS tumor

tag: mouse model line where the tumor is sampled


title: N30_H3K36me3


title: N26_input

value: input


title: R0.5-3


title: R0.5-2


title: H3


title: S3


title: S1


title: Tumor_fibroblast_rep2

value: 78 weeks


title: BackSkin_fibroblast_rep2


title: N30_input


title: N30_H3K4me1


title: H2


title: N30_H3K27me3


title: N28_input


title: N28_H3K36me3


title: N28_H3K27me3


title: N28_H3K27ac


title: N26_H3K4me1


title: N30_H3K27ac


title: 2N15_H3K27ac


title: 1N15_H3K36me3


title: 1N15_H3K27me3


title: 1N15_H3K27ac


title: Wildtype_16wks_methylseq_0

city: Piscataway

first: Renyi

last: Wu

state: NJ

address: 160 Frelinghuysen road

department: Pharmacy School

institute: Rutgers University

data_processing:
Illumina standard procedure for basecalling.
The reads were aligned to the in silico C-T mouse genome (mm10) with the Bismark algorithm.
Deduplication by Picard
Single base-pair resolution methylation coverage was counted by DMRfinder package
CpG sites were clustered into DMRs (differentially methylated regions) with DMRfinder; differentially methylation analysis with DSS R/Bioconductor package.
Genome_build: mm10
Supplementary_files_format_and_content: Tab delimited tables of counts of methylation and unmethylation for each CpG site.

source_name: vental and dorsolateral prostate

extract_protocol:
Tissues were snap frozen in liquid nitrogen for long-term storage. RNA were extracted using Qiagen AllPRep DNA/RNA extraction kit.
DNA libraries were prepared for sequencing using Agilent SureSelectXT Mouse Methyl-Seq system. (Catlog number 931052)

value: prostate

tag: diet

value: Control

value: 16 weeks


title: Wildtype_24wks_methylseq_1

value: 24 weeks


title: N26_H3K27me3


title: Wildtype_24wks_methylseq_0


title: N26_H3K27ac


title: TRAMP_16wks_methylseq_1


title: TRAMP+PEITC_16wks_methylseq_0

value: PEITC


title: TRAMP+PEITC_24wks_methylseq_1


title: 2N15_input


title: N26_H3K36me3


title: TRAMP+PEITC_24wks_methylseq_0


title: 2N15_H3K36me3


title: TRAMP_24wks_methylseq_2


title: Wildtype_16wks_rnaseq_0

data_processing:
Illumina standard procedure for basecalling.
Reads were aligned to mouse genome (mm10) using Hisat2 with default parameters.
Deduplication by Picard
Reads overlapping genes features were quantitatived by FeatureCounts
Differentially expression and FPKM calculation by R/Bioconductor package DESeq2
Genome_build: mm10
Supplementary_files_format_and_content: tab delimited tableswith FPKM values for each sample.

extract_protocol:
Tissues were snap frozen in liquid nitrogen for long-term storage. RNA were extracted using Qiagen AllPRep DNA/RNA extraction kit.
RNA libraries were prepared for sequencing using standard Illumina protocols


title: TRAMP_24wks_methylseq_0


title: TRAMP_24wks_rnaseq_t

source_name: prostate tumor (original lobe unknown)

value: prostate tumor


title: 2N15_H3K27me3


title: Wildtype_24wks_rnaseq_1


title: 1N15_input


title: Wildtype_24wks_rnaseq_0


title: TRAMP+PEITC_16wks_rnaseq_1


title: 1N15_H3K4me1


title: 2N15_H3K4me1


title: TRAMP+PEITC_16wks_rnaseq_0


title: TRAMP+PEITC_24wks_rnaseq_1


title: TRAMP_24wks_methylseq_t


title: G1ER_WT_PolII-phospho-serineII Input rep1

city: Boston

first: Leonard

last: Zon

state: MA

address: 1 Blackfan Circle

department: Oncology/Hematology

institute: Boston Children's Hospital

data_processing:
Sequences were aligned to UCSC build version GRCm38 version of mouse genome using Bowtie2 (version 2.2.1)  (Langmead et al., 2012) with the following parameters: --end-to-end, -N0, -L20.
MACS2 version 2.1.0 (Zhang et al., 2008) peak finding algorithm was used to identify regions of ChIP-Seq peaks, with a q-value threshold of enrichment of 0.05 for all datasets.
Genome_build: GRCm38/mm10
Supplementary_files_format_and_content: NarrowPeak bed files of binding peaks.

source_name: G1ER cell line

growth_protocol: Wild type and CHD7 knockout G1ER cells were grown and maintained in IMDM media containing heat-inactivated FBS (16%), pen/strep (2%), monothioglycerol (6.2 mL per 500 mL), EPO (2U/mL) and SCF conditioned media (0.6%). Cells were differentiated with beta-estradiol (10-7M final concentration). After 48 hrs of beta-estradiol treatment, differentiation was assessed using benzidine staining method (using o-diansidine from Sigma, D9143, ref – Orkin SH, Harosi FI, Leder P. PNAS 72 (1): 98-102, 1975).

extract_protocol:
Whole cell extracts were sonicated to solubilize the chromatin and target bound fragments were isolated with relevant antibody
The chromatin extracts containing DNA fragments with an average size of 500 bp were immunoprecipitated using different antibodies. Purified immunoprecipitated DNA were prepared for sequencing according to a modified version of the Solexa Genomic DNA protocol. Fragmented DNA was end repaired and subjected to 18 cycles of LM-PCR using oligos provided by Illumina. Amplified fragments between 150 and 300bp (representing shear fragments between 50 and 200nt in length and ~100bp of primer sequence) were isolated by agarose gel electrophoresis and purified.

tag: cell line

value: erythroblasts

value: wild-type

tag: antibody


title: G1ER_CHD7-KO_PolII-phospho-serineII rep2

value: PolII-phospho serine II (abcam ab5095)


title: G1ER_WT_Input


title: G1ER_CHD7-Input


title: G1ER_CHD7-KO_PolII-phospho-serineII Input rep1


title: G1ER_CHD7-KO_PolII-phospho-serineII Input rep2


title: TRAMP+PEITC_16wks_methylseq_1


title: G1ER_CHD7-KO_RUNX1

value: RUNX1 (Abcam, ab23980)


title: G1ER_CHD7-KO_50K_1 [ATAC-seq]

data_processing:
All ATAC-Seq datasets were aligned to build version GRCm38/mm9 of the mouse genome using Bowtie2 (version 2.2.1)  (Langmead et al., 2012) with the following parameters: --end-to-end, -N0, -L20. We used the MACS2 version 2.1.0 (Zhang et al., 2008) peak finding algorithm to identify regions of ATAC-Seq peaks, with the following parameter --nomodel --shift -100 --extsize 200. A q-value threshold of enrichment of 0.05 was used for all datasets.
Genome_build: GRCm38/mm10

growth_protocol: Wild type and CHD7 knockout G1ER cells were grown and maintained in IMDM media containing heat-inactivated FBS (16%), pen/strep (2%), monothioglycerol (6.2 mL per 500 mL), EPO (2U/mL) and SCF conditioned media (0.6%).

extract_protocol:
Cell lysates were treated with transposase (Illumina Nextera kit, 15028252) for 1-2 hours at 37C. Following transposition, DNA was purified using MinElute PCR purification kit (Qiagen, 28004).
Libraries were constructed according to Illumina protocol using transposase treated DNA, NEB PCR master mix, SYBR green, universal and library-specific Nextera index primers. Libraries were analyzed on an Agilent Bioanalyzer and sequenced using Illumina Hiseq 2500.

tag: tissue source

tag: cell number

value: 1 hour


title: G1ER_WT_50K_1 [ATAC-seq]


title: TRAMP_16wks_methylseq_0


title: G1ER_CHD7-KO_GATA2

value: GATA2 (Santa Cruz, sc9008)


title: G1ER_WT_RUNX1


title: G1ER_CHD7-KO_bE_0h

description:
ribosomal depleted RNA
AC2-E4-bE

data_processing:
Quality control of RNA-Seq datasets was performed by FastQC and Cutadapt to remove adaptor sequences and low quality regions.
High-quality reads were aligned to UCSC build GRCm38 of the mouse genome using Tophat 2.0.11 without novel splicing form calls.
Transcript abundance and differential expression were calculated with Cufflinks 2.2.1.
FPKM values were used to normalize and quantify each transcript.
Genome_build: GRCm38/mm10
Supplementary_files_format_and_content: Excel summary file of transcript abundance.

growth_protocol: Wild type and CHD7 knockout G1ER cells were grown and maintained in IMDM media containing heat-inactivated FBS (16%), pen/strep (2%), monothioglycerol (6.2 mL per 500 mL), EPO (2U/mL) and SCF conditioned media (0.6%). Cells were differentiated with beta-estradiol (10-7M final concentration). After 24 hrs of beta-estradiol treatment, differentiation was assessed using benzidine staining method (using o-diansidine from Sigma, D9143, ref – Orkin SH, Harosi FI, Leder P. PNAS 72 (1): 98-102, 1975).

extract_protocol:
RNA from one million cells was isolated with Trizol according to the manufacturer’s instructions. The RNA was DNAse treated using the RNase free DNase set from Qiagen (79254) according to the instructions. The whole amount of RNA was treated with the Ribo-Zero Gold kit (Human/Mouse/Rat, Epicentre) according to the manufacturer’s instructions.
Ribo-zero treated RNA was used to create multiplexed RNA-seq libraries using the ScriptSeq™ v2 RNA-Seq Library Preparation Kit (Epicentre) according to the manufacturer’s instructions.

treatment_protocol: G1ER cells were differentiated with beta-estradiol for 24 hours.

value: estradiol


title: G1ER_WT_bE_0h

description:
ribosomal depleted RNA
AC1-G1ER-bE


title: G1ER_WT_bE_24h

description:
ribosomal depleted RNA
AC4-G1ERplus-bE


title: mASPS_2_input

data_processing:
single-end 50bp sequencing on an Illumina MiSeq platform
ChIP-seq reads were aligned to the mm10 genome assembly using BWA-MEM (version 0.7.10-r789)
peaks were called using MACS2 with following settings: -B --SPMR --pvalue=1e-2 --mfold 15 100
Genome_build: mm10
Supplementary_files_format_and_content: bigwig files were generated using MACS2; Scores represent a normalized pileup signal

source_name: mouse tumor

extract_protocol:
Nuclei isolated from snap frozen samples. Lysates were clarified from sonicated nuclei and TARGET DNA complexes were isolated with antibody.
Library was prepared using NEBnext ChIP-seq libraray prep reagent set

value: ASPSCR1-TFE3 mouse model

value: mouse tumor


title: mASPS_1_input


title: G1ER_WT_PolII-phospho-serineII rep2


title: TRAMP+PEITC_24wks_rnaseq_0


title: TRAMP_24wks_rnaseq_2


title: TRAMP_16wks_rnaseq_1


title: mASPS_3_ASPSCR1


title: TRAMP_16wks_rnaseq_0


title: G1ER_WT_PolII-phospho-serineII Input rep2


title: G1ER_WT_PolII-phospho-serineII rep1


title: mASPS_3_input


title: G1ER_CHD7-KO_50K_2 [ATAC-seq]

value: 2 hours


title: TRAMP_24wks_rnaseq_0


title: G1ER_WT_GATA2


title: G1ER_CHD7-KO_PolII-phospho-serineII rep1


title: G1ER_WT_50K_2 [ATAC-seq]


title: Bone marrow MPP, rep 2

type: RNA

description:
Gene expression of sorted mouse bone marrow MPP
No treatment

hyb_protocol: Samples were hybridized to Mouse Gene 2.0 ST Arrays (Affymetrix) and scanned at the Microarray Core Facility at the IDDRC of Boston Children's Hospital and Harvard Medical School.

scan_protocol: Scanning was done per manufacturer's instructions.

data_processing: Raw data were processed using the Affymetrix Command Console Software to determine RMA-normalized expression values (log2) and used to identify differences in gene expression between GFP (+) and (-) samples.

label: biotin

source_name: CD48+CD150-CD34+Lin-Sca1+Kit+ MPPs sorted from C57/Bl6 mice

label_protocol: Amplification and library preparation was performed using the Ovation Pico WTA System V2 and Encore Biotin Module labeling.

extract_protocol: Trizol extraction of total RNA was performed according to the manufacturer's instructions.

treatment_protocol: Cells were sorted from mouse whole bone marrow, treated with DMSO for 2 hours, then transplanted into primary recipients. Cells were isolated from primary recipients one year later then transplanted into secondary recipients, without further treatment. After 3 months, cells were isolated from secondary recipients and used for total RNA extraction.

value: bone marrow

value: MPP

value: 8 week

tag: cell markers


title: G1ER_CHD7-KO_bE_24h

description:
ribosomal depleted RNA
AC5-E4plus

tag: cell type


title: mASPS_3_RNAPol2

tag: genotype

value: POLII


title: mASPS_1_ASPSCR1


title: mASPS_2_ASPSCR1


title: Bone marrow MPP, rep 1


title: BT969_S37

city: Hamilton

first: Sheila

last: Singh

state: ON

address: 1280 Main Street West

department: Biochemistry and Biomedical Sciences

country: Canada

institute: McMaster University

data_processing:
alignment
Assembly: GRCh38 (no alt.)

organism: Homo sapiens

source_name: Melanoma to brain metastasis cells

growth_protocol: Brain metastases (BM) originating from breast and melanoma primary samples were processed and maintained in Stem cell media (75% Dulbecco's Modified Eagle Medium (DMEM) GlutaMAXTM (#10567014,Thermo Fisher Scientific) and 25% F12 GlutaMAXTM (#31765035; Gibco) as Brain metastasis initiating cells (BMICs). BMICs were grown as tumorspheres that were maintained at 37°C with a humidified atmosphere of 5% CO2

extract_protocol:
RNA was extracted from BMICs using Norgen total RNA kit.
Library prep was performed using the NEBNext Ultra Directional RNA Library Prep Kit along with the NEBNext Poly(A) mRNA Magnetic Isolation Module. Libraries were subject to further BioAnalyzer QC and quantified by qPCR.

tag: bmic line

tag: sample id

tag: sample type

value: original


title: Bone marrow LT-HSC, rep 1

description:
Gene expression of sorted mouse bone marrow LT-HSC
No treatment

source_name: CD48-CD150+CD34-Lin-Sca1+Kit+ LT-HSCs sorted from C57/Bl6 mice

tag: strain

value: LT-HSC


title: SCB-2_S36

value: BMSC


title: SCB-1_S35


title: FPB-3_S32

source_name: Breast to brain metastasis cells

value: BMFP


title: SCB-4_S39


title: N1IC+β-Catenin-KO cells, biological rep2

city: Barcelona

first: Christos

last: Gekas

address: Carrer del Doctor Aiguader 88, Lab277

country: Spain

institute: Centre for Genomic Regulation (CRG)

description: Gene expression data from fetal liver LSK cells in N1IC+β-Catenin-knockout E14.5 embryos

hyb_protocol: cRNA was hybridized on  Affymetrix GeneChip® Mouse Gene 2.0 ST arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.

scan_protocol: GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.

data_processing: CEL files were analyzed in R (v3.1.2) with the oligo (v1.30.0) and limma (v3.22.4) packages from Bioconductor, data were normalized using RMA (Robust Multi-array Average) and expression values log2-transformed.

source_name: E14.5 FL LSK cells

label_protocol: 7 ug total RNA was labelled with the FL-Ovation™ cDNA Biotin Module V2 kit (NuGEN Technologies).

extract_protocol: RNA was extracted with RNEasy Micro kit (QIAGen) according to manufacturer's instructions and amplified using the Ovation™ Pico WTA System (NuGEN Technologies, San Carlos, CA) and sense transcript cDNA (ST-cDNA) was generated using the WT-Ovation™ Exon Module (NuGEN Technologies)


title: N1IC+β-Catenin-KO cells, biological rep3


title: Bone marrow ST-HSC, rep 2

description:
Gene expression of sorted mouse bone marrow ST-HSC
No treatment

source_name: CD48-CD150+CD34+Lin-Sca1+Kit+ ST-HSCs sorted from C57/Bl6 mice

tag: tissue

value: ST-HSC


title: Bone marrow ST-HSC, rep 3

tag: age


title: β-Catenin-KO cells, biological rep3

description: Gene expression data from fetal liver LSK cells in β-Catenin-knockout E14.5 embryos

value: VavCre(+)β-Catenin(fl/fl)


title: BT925_S34


title: FPB-2_S30


title: Bone marrow LT-HSC, rep 3


title: Bone marrow ST-HSC, rep 1


title: N1IC+ cells, biological rep2

description: Gene expression data from fetal liver LSK cells (Leukemic Initiating Cells) in N1IC+ E14.5 embryos


title: β-Catenin-KO cells, biological rep2


title: BT923_S31


title: wild-type cells, biological rep1

description: Gene expression data from fetal liver LSK cells in wild-type E14.5 embryos

value: VavCre(+)β-Catenin(fl/+)


title: N1IC+β-Catenin-KO cells, biological rep1


title: N1IC+ cells, biological rep3


title: β-Catenin-KO cells, biological rep1


title: N1IC+ cells, biological rep1


title: SCB-3_S38


title: Bone marrow MPP, rep 3


title: wild-type cells, biological rep2


title: FPB-1_S29


title: wild-type cells, biological rep3


title: BT922_S28


title: Pericyte_iPSC_D05_s3

city: Cambridge

first: Baohong

last: Zhang

address: 225 Binney St

institute: Biogen Inc.

description:
Mesoderm-derived
Batch10.TPM.csv
sample115

data_processing:
The raw fastqs was mapped to the hg38 genome using START, and gene quantification TPM was obtained by RSEM.
Assembly: GRCh38
Supplementary files format and content: CSV/TPM

source_name: iPSC

growth_protocol: The iPSC-derived Pericyte followed Fall 2019 publication (PMID: 30745035)

extract_protocol: NOT PROVIDED; REQUESTED

value: Pericyte

tag: batch

tag: time


title: Pericyte_iPSC_D10_s1

description:
Mesoderm-derived
Batch10.TPM.csv
sample116


title: Pericyte_iPSC_D05_s1

description:
Mesoderm-derived
Batch10.TPM.csv
sample113


title: sample109_Oligodendrocytes_iPSC_TFDRep3-OPC-Day34O4

description: processed data file: Batch9.TPM.csv

data_processing:
The raw fastqs was mapped to the hg38 genome using START, and gene quantification TPM was obtained by RSEM.
Genome_build: GRCh38
Supplementary_files_format_and_content: Gene Expression TPM, csv

value: Oligodendrocytes


title: Pericyte_iPSC_D10_s2

description:
Mesoderm-derived
Batch10.TPM.csv
sample117


title: sample106_Oligodendrocytes_iPSC_TFDRep3-OPC-Day28O4

description: processed data file: Batch9.TPM.csv


title: sample107_Oligodendrocytes_iPSC_TFDRep1-OPC-Day34O4

description: processed data file: Batch9.TPM.csv


title: Pericyte_iPSC_D05_s2

description:
Mesoderm-derived
Batch10.TPM.csv
sample114


title: Pericyte_iPSC_D00_s1

description:
Mesoderm-derived
Batch10.TPM.csv
sample110


title: Pericyte_iPSC_D00_s2

description:
Mesoderm-derived
Batch10.TPM.csv
sample111


title: Pericyte_iPSC_D00_s3

description:
Mesoderm-derived
Batch10.TPM.csv
sample112


title: sample105_Oligodendrocytes_iPSC_TFDRep3-OPC-Day21O4

description: processed data file: Batch9.TPM.csv


title: sample108_Oligodendrocytes_iPSC_TFDRep2-OPC-Day34O4

description: processed data file: Batch9.TPM.csv


title: FPB-4_S33


title: sample101_Oligodendrocytes_iPSC_TFDRep1-OPC-Day21O4

description: processed data file: Batch9.TPM.csv


title: sample104_Oligodendrocytes_iPSC_TFDRep2-OPC-Day27O4

description: processed data file: Batch9.TPM.csv


title: sample102_Oligodendrocytes_iPSC_TFDRep1-OPC-Day27O4

description: processed data file: Batch9.TPM.csv


title: sample91_Neuron_iPSC_CDI-iDopaneurons-suspension-3

description: processed data file: Batch7_Vendor.TPM.csv

value: Neuron


title: sample99_Oligodendrocytes_iPSC_OPC-CD-Day76-All-Rep1

description: processed data file: Batch8.TPM.csv


title: sample93_Neuron_iPSC_TCS-Dopaneurons-suspension-2

description: processed data file: Batch7.TPM.csv


title: sample97_Oligodendrocytes_iPSC_OPC-CD-Day69-All-Rep1

description: processed data file: Batch8.TPM.csv


title: sample100_Oligodendrocytes_iPSC_OPC-CD-Day76-O4-Rep1

description: processed data file: Batch8.TPM.csv


title: sample95_Oligodendrocytes_iPSC_OPC-CD-Day62-All-Rep1

description: processed data file: Batch8.TPM.csv


title: sample96_Oligodendrocytes_iPSC_OPC-CD-Day62-O4-Rep1

description: processed data file: Batch8.TPM.csv


title: sample90_Neuron_iPSC_CDI-iDopaneurons-suspension-2


title: Pericyte_iPSC_D10_s3

description:
Mesoderm-derived
Batch10.TPM.csv
sample118


title: sample94_Neuron_iPSC_TCS-Dopaneurons-suspension-3

description: processed data file: Batch7.TPM.csv


title: sample89_Neuron_iPSC_CDI-iDopaneurons-suspension-1


title: sample88_Neuron_iPSC_I3-DIV28-3

description: processed data file: Batch6.TPM.csv


title: sample92_Neuron_iPSC_TCS-Dopaneurons-suspension-1

description: processed data file: Batch7.TPM.csv


title: sample87_Neuron_iPSC_I3-DIV28-2

description: processed data file: Batch6.TPM.csv


title: sample85_Neuron_iPSC_I2-DIV28-3

description: processed data file: Batch6.TPM.csv


title: sample81_Neuron_iPSC_I1-DIV28-2

description: processed data file: Batch6.TPM.csv


title: sample79_Neuron_iPSC_H2-DIV28-3

description: processed data file: Batch6.TPM.csv


title: sample86_Neuron_iPSC_I3-DIV28-1

description: processed data file: Batch6.TPM.csv


title: sample103_Oligodendrocytes_iPSC_TFDRep2-OPC-Day21O4

description: processed data file: Batch9.TPM.csv


title: sample78_Neuron_iPSC_H2-DIV28-2

description: processed data file: Batch6.TPM.csv


title: sample83_Neuron_iPSC_I2-DIV28-1

description: processed data file: Batch6.TPM.csv


title: sample82_Neuron_iPSC_I1-DIV28-3

description: processed data file: Batch6.TPM.csv


title: sample80_Neuron_iPSC_I1-DIV28-1

description: processed data file: Batch6.TPM.csv


title: sample77_Neuron_iPSC_H2-DIV28-1

description: processed data file: Batch6.TPM.csv


title: sample98_Oligodendrocytes_iPSC_OPC-CD-Day69-O4-Rep1

description: processed data file: Batch8.TPM.csv


title: sample75_Neuron_iPSC_H1-DIV28-2

description: processed data file: Batch6.TPM.csv


title: sample68_Neuron_iPSC_I2-DIV21-1

description: processed data file: Batch6.TPM.csv


title: sample71_Neuron_iPSC_I3-DIV21-1

description: processed data file: Batch6.TPM.csv


title: sample76_Neuron_iPSC_H1-DIV28-3

description: processed data file: Batch6.TPM.csv


title: sample74_Neuron_iPSC_H1-DIV28-1

description: processed data file: Batch6.TPM.csv


title: sample64_Neuron_iPSC_H2-DIV21-3

description: processed data file: Batch6.TPM.csv


title: sample61_Neuron_iPSC_H1-DIV21-3

description: processed data file: Batch6.TPM.csv


title: sample60_Neuron_iPSC_H1-DIV21-2

description: processed data file: Batch6.TPM.csv


title: sample59_Neuron_iPSC_H1-DIV21-1

description: processed data file: Batch6.TPM.csv


title: sample57_Neuron_iPSC_I3-DIV14-2

description: processed data file: Batch6.TPM.csv


title: sample72_Neuron_iPSC_I3-DIV21-2

description: processed data file: Batch6.TPM.csv


title: sample69_Neuron_iPSC_I2-DIV21-2

description: processed data file: Batch6.TPM.csv


title: sample55_Neuron_iPSC_I2-DIV14-3

description: processed data file: Batch6.TPM.csv


title: sample65_Neuron_iPSC_I1-DIV21-1

description: processed data file: Batch6.TPM.csv


title: sample52_Neuron_iPSC_I1-DIV14-3

description: processed data file: Batch6.TPM.csv


title: sample67_Neuron_iPSC_I1-DIV21-3

description: processed data file: Batch6.TPM.csv


title: sample51_Neuron_iPSC_I1-DIV14-2

description: processed data file: Batch6.TPM.csv


title: sample62_Neuron_iPSC_H2-DIV21-1

description: processed data file: Batch6.TPM.csv


title: sample84_Neuron_iPSC_I2-DIV28-2

description: processed data file: Batch6.TPM.csv


title: sample58_Neuron_iPSC_I3-DIV14-3

description: processed data file: Batch6.TPM.csv


title: sample66_Neuron_iPSC_I1-DIV21-2

description: processed data file: Batch6.TPM.csv


title: sample63_Neuron_iPSC_H2-DIV21-2

description: processed data file: Batch6.TPM.csv


title: sample46_Neuron_iPSC_H1-DIV14-3

description: processed data file: Batch6.TPM.csv


title: sample56_Neuron_iPSC_I3-DIV14-1

description: processed data file: Batch6.TPM.csv


title: sample47_Neuron_iPSC_H2-DIV14-1

description: processed data file: Batch6.TPM.csv


title: sample54_Neuron_iPSC_I2-DIV14-2

description: processed data file: Batch6.TPM.csv


title: sample42_Neuron_iPSC_I3-DIV7-2

description: processed data file: Batch6.TPM.csv


title: sample73_Neuron_iPSC_I3-DIV21-3

description: processed data file: Batch6.TPM.csv


title: sample70_Neuron_iPSC_I2-DIV21-3

description: processed data file: Batch6.TPM.csv


title: sample53_Neuron_iPSC_I2-DIV14-1

description: processed data file: Batch6.TPM.csv


title: sample40_Neuron_iPSC_I2-DIV7-3

description: processed data file: Batch6.TPM.csv


title: sample38_Neuron_iPSC_I2-DIV7-1

description: processed data file: Batch6.TPM.csv


title: sample39_Neuron_iPSC_I2-DIV7-2

description: processed data file: Batch6.TPM.csv


title: sample37_Neuron_iPSC_I1-DIV7-3

description: processed data file: Batch6.TPM.csv


title: sample36_Neuron_iPSC_I1-DIV7-2

description: processed data file: Batch6.TPM.csv


title: sample49_Neuron_iPSC_H2-DIV14-3

description: processed data file: Batch6.TPM.csv


title: sample50_Neuron_iPSC_I1-DIV14-1

description: processed data file: Batch6.TPM.csv


title: sample33_Neuron_iPSC_H2-DIV7-2

description: processed data file: Batch6.TPM.csv


title: sample48_Neuron_iPSC_H2-DIV14-2

description: processed data file: Batch6.TPM.csv


title: sample45_Neuron_iPSC_H1-DIV14-2

description: processed data file: Batch6.TPM.csv


title: sample43_Neuron_iPSC_I3-DIV7-3

description: processed data file: Batch6.TPM.csv


title: sample44_Neuron_iPSC_H1-DIV14-1

description: processed data file: Batch6.TPM.csv


title: sample41_Neuron_iPSC_I3-DIV7-1

description: processed data file: Batch6.TPM.csv


title: sample28_Microglia_iPSC_3M-microglia-21d-3

description: processed data file: Batch5.TPM.csv

value: Microglia


title: sample25_Astrocytes_iPSC_A4-iAstrocytes-Vehicle-24h

description: processed data file: Batch4.TPM.csv

value: Astrocytes


title: sample23_Astrocytes_iPSC_A2-iAstrocytes-Vehicle-24h

description: processed data file: Batch4.TPM.csv


title: sample21_Astrocytes_iPSC_A16-iAstrocytes-Vehicle-48h

description: processed data file: Batch4.TPM.csv


title: sample24_Astrocytes_iPSC_A3-iAstrocytes-Vehicle-24h

description: processed data file: Batch4.TPM.csv


title: sample30_Neuron_iPSC_H1-DIV7-2

description: processed data file: Batch6.TPM.csv


title: sample16_Astrocytes_iPSC_Astrocytes-TCSmedia-d50-2

description: processed data file: Batch3.TPM.csv


title: sample31_Neuron_iPSC_H1-DIV7-3

description: processed data file: Batch6.TPM.csv


title: sample32_Neuron_iPSC_H2-DIV7-1

description: processed data file: Batch6.TPM.csv


title: sample29_Neuron_iPSC_H1-DIV7-1

description: processed data file: Batch6.TPM.csv


title: sample34_Neuron_iPSC_H2-DIV7-3

description: processed data file: Batch6.TPM.csv


title: sample27_Microglia_iPSC_3M-microglia-21d-2

description: processed data file: Batch5.TPM.csv


title: sample12_Astrocytes_iPSC_Astrocytes-SCImedia-d70-1

description: processed data file: Batch3.TPM.csv


title: sample35_Neuron_iPSC_I1-DIV7-1

description: processed data file: Batch6.TPM.csv


title: sample20_Astrocytes_iPSC_A15-iAstrocytes-Vehicle-48h

description: processed data file: Batch4.TPM.csv


title: sample19_Astrocytes_iPSC_A14-iAstrocytes-Vehicle-48h

description: processed data file: Batch4.TPM.csv


title: sample26_Microglia_iPSC_3M-microglia-21d-1

description: processed data file: Batch5.TPM.csv


title: sample9_Pericyte_Primary_HBVP-s1

description: processed data file: Batch2.TPM.csv

source_name: Primary


title: sample22_Astrocytes_iPSC_A1-iAstrocytes-Vehicle-24h

description: processed data file: Batch4.TPM.csv


title: sample18_Astrocytes_iPSC_A13-iAstrocytes-Vehicle-48h

description: processed data file: Batch4.TPM.csv


title: sample15_Astrocytes_iPSC_Astrocytes-TCSmedia-d50-1

description: processed data file: Batch3.TPM.csv


title: sample17_Astrocytes_iPSC_Astrocytes-TCSmedia-d50-3

description: processed data file: Batch3.TPM.csv


title: sample13_Astrocytes_iPSC_Astrocytes-SCImedia-d70-2

description: processed data file: Batch3.TPM.csv


title: sample14_Astrocytes_iPSC_Astrocytes-SCImedia-d70-3

description: processed data file: Batch3.TPM.csv


title: sample10_Pericyte_Primary_HBVP-s2

description: processed data file: Batch2.TPM.csv


title: sample6_Endothelial_Primary_TY10s1

description: processed data file: Batch1.TPM.csv

value: Endothelial


title: sample11_Pericyte_Primary_HBVPs-3

description: processed data file: Batch2.TPM.csv


title: sample7_Endothelial_Primary_TY10s2

description: processed data file: Batch1.TPM.csv


title: sample8_Endothelial_Primary_TY10s3

description: processed data file: Batch1.TPM.csv


title: sample5_Endothelial_Primary_pHBMECs3

description: processed data file: Batch1.TPM.csv


title: shCon

city: Foshan

first: Wanqin

last: Liao

address: 5# Hebin Road, Chancheng

institute: Foshan University

data_processing:
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to hg19 whole genome using tophat v2.0.9 with parameters -p 8 -q 2
Reads per kilobase of exon per megabase of library size (RPKM) and differential expression were calculated against ensemble gene annotations using cuffdiff program with default options and masking repeats.
Genome_build: hg19
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample

source_name: human breast cancer cell line

growth_protocol: Cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) (Gibco, Grand Island, NY, USA) containing 1% penicillin/streptomycin and 10% FBS. Regular cell cultures were maintained at 37℃ with 5% CO2.

extract_protocol:
Total RNA from MDA-MB-231 cells was isolated using Trizol Reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s introductions.
RNA libraries were prepared for sequencing using standard Illumina protocols.

treatment_protocol: MDA-MB-231 cells were transduced with lentivirus harboring control shRNA and shRNA targeting MPP7, at a multiplicity of infection (MOI) of 10. The medium was refreshed 12 h post-infection. The infection efficiency was monitored by fluorescence microscopy 72 h after infection. After selection with 1 mg/ml puromycine for 7 days, stable cell lines with MPP7 depletion were established.

value: human breast cancer

value: Control


title: sample4_Endothelial_Primary_pHBMECs2

description: processed data file: Batch1.TPM.csv


title: RNA-Seq_VascAN_Pt2

city: Kuopio

first: Minna

middle: U

last: Kaikkonen

department: A.I. Virtanen Institute, Department of Biotechnology and Molecular Medicine

country: Finland

institute: University of Eastern Finland

description:
vascular malformation
Gene_Expression_RPKM.txt

data_processing:
Libraries were sequenced for 50 cycles on an Illumina HiSeq 2000 according to the manufacturer’s instructions.
RNA-Seq results were trimmed to remove 3' A-stretches originating from the library preparation and poor quality reads were filtered out (minimum 97% of bp over quality cutoff 10).
Reads were aligned to the hg19 genome using Tophat.
Data analysis was performed using HOMER and the detailed instructions for analysis can be found at http://homer.salk.edu/homer/
Genome_build: hg19
Supplementary_files_format_and_content: Gene expression values (RPKM) and UCSC Bedgraphs are provided

molecule: polyA RNA

source_name: Human CD31+ endothelial cells extracted from vascular malformation

growth_protocol: Resected patient tissue samples were treated with collagenase type II (Worthington, Lakewood, NJ) for an hour at 37⁰C under agitation. Selection of ECs was performed with CD31 MicroBead Kit and a magnetic column (Milltenyi Biotec, Bergisch Gladbach, Germany) as previously described (Partanen et al, Angiogenesis. 2013;16(1):137-146). Patient-derived ECs were cultured on fibronectin/gelatin coated cell culture flasks in Endothelial Cell Growth medium (EGM; Cambrex Biosciences, East Rutherford, NJ) supplemented with 20 % FBS. Patient-derived fibroblasts from flow-through fraction were maintained in DMEM supplemented with 10 % FBS and antibiotics and determined as fibroblasts by cell morphology and western blot analysis by using EC, SMC and FB markers. HUVECs were isolated from human umbilical cords with collagenase type IV (Worthington) treatment as previously described (Jaffe et al, J Clin Invest. 1973;52(11):2745-2756) and maintained in EGM. HsaVECs as well as control human pulmonary fibroblasts (HPF-c) were obtained from PromoCell (Heidelberg, Germany) and maintained according to manufacturer’s instructions in EGM supplemented with 20 % FBS or in DMEM supplemented with 10 % FBS and antibiotics, respectively. Selection of control ECs was performed by CD31 MicroBead Kit.

extract_protocol:
RNA was purified using RNeasy Mini Kit and enriched for Poly(A)-RNA with MicroPoly(A) Purist Kit.
The RNA was base-hydrolyzed, dephosphorylated with PNK and purified using RNA Clean & Concentrator kit (Zymo). Poly(A)-tailing was followed by cDNA synthesis using complementary poly(T)-primers containing Illumina adapter sequences. Excess oligo was removed by Exonuclease I and cDNA fragments of were purified using ChIP DNA Clean & Concentrator kit. The recovered cDNA was RNaseH treated and circularized (CircLigase) and amplified for 10 cycles. The final product was ran on 10% TBE gel, gel purified (180-350 bp) and cleaned-up using ChIP DNA clean & Concentrator Kit.

tag: replicate

value: patient 2


title: RNA-Seq_VascAN_Pt5

value: CD31+ endothelial cells extracted from vascular malformation

value: patient 5


title: sample3_Endothelial_Primary_pHBMECs1

description: processed data file: Batch1.TPM.csv


title: RNA-Seq_HsaVEC_Pt2

description:
control
Gene_Expression_RPKM.txt

source_name: Human saphenous vein endothelial cells

value: saphenous vein endothelial cells

value: replicate 2


title: shMPP7

value: MPP7 Knockdown


title: sample2_Endothelial_Primary_HUVECs2

description: processed data file: Batch1.TPM.csv


title: sample1_Endothelial_Primary_HUVECs1

description: processed data file: Batch1.TPM.csv


title: UF_KS_044_S8_combined

city: Newcastle Upon Tyne

first: Konstantinos

last: Stellos

state: England

address: Central Parkway, Centre for Life

department: Faculty of Medical Sciences

country: United Kingdom

institute: Newcastle University

data_processing:
The resulting raw reads were assessed for quality, adapter content and duplication rates with FastQC (Andrews S. 2010, FastQC: a quality control tool for high throughput sequence data. Available online at: http://www.bioinformatics.babraham.ac.uk/projects/fastqc).
The raw read data was processed to optionally remove an adapter sequence and trim according to a quality threshold (default Q>=20) by cutadapt. Only reads of the desired size range are selected to limit the pool to likely mature miRNAs (default: 18-28 nt).
Duplicate reads are collapsed to decrease the number of necessary homology searches. Only those sequences present in a minimum number (default = 5) are kept for further analyses.
Next, read counts from isomiRs of the same miRNA are combined. These isomiR read sequences may only differ by the 3' end and are thus putatively encoded by the same gene and bear the same target specificity. This function allows the summary of putatively functionally equivalent isomiRs resulting from imperfect digestion by the RNases Drosha and Dicer or RNA-Editing by specialized enzymes resulting in 3' modification. Only the final 3' nucleotide may differ between two sequences to be counted as isoforms of the same miRNA and only the longest isoform sequence is used in the next step to reduce the amount of homology searches per miRNA.
The resulting read sequences are compared versus human mirBase database of miRNAs. Sensitivity and specificity of this BLASTN homology search can be controlled using various parameters inside the mirpipe.pl script. Parameters are optimized for small query sequences (-num_alignments 15 -word_size 7 -evalue 10 -dust no -strand plus). The resulting hits are filtered to exclude those with too many mismatches ((read length - alignment length) + mismatches + gaps = final mismatches).
Detected reference miRNA families per read are scored based on the minimum number of mismatches. If a read matched equally versus multiple miRNA families the respective families are joined by single linkage clustering. By default only those read sequences that are at least 5% as abundant as the most abundant sequence per miRNA family cluster are denoted.
Genome_build: hg19
Supplementary_files_format_and_content: library size normalized counts per sample

source_name: human umbilical vein endothelial cells

growth_protocol: Pooled HUVECs from human donors were purchased from Lonza and cultured in endothelial basal medium (EBM;Lonza) supplemented with EGM-SingleQuots (Lonza) and 10% fetal calf serum (FCS; Invitrogen (San Diego, CA ) until the third passage. The authentication and negative result from mycoplasma testing were certified by Lonza.

extract_protocol:
Total RNA was extracted using the miRNeasy Mini Kit (Qiagen) according to the manufacturer's protocol. The cDNA and the final sequencing libraries were prepared as described before (Wang et.al, EMBO Mol Med, 2013). It has to be noted that the resulting double-stranded cDNA fragments do not preserve information about the strand specificity of the original transcript. The libraries were sequenced using the Illumina HiSeq 2000 for 1 x 51cycles following the standard protocol.
According to the protocol of TruSeq Small RNA kit manufacturer

treatment_protocol: HUVECs were transfected at 70% confluency using Lipofectamine RNAiMAX according to the manufacturer's protocol with a final concentration of 55nM siRNA. The following siRNA sequences were used: Scrambled (5’-UCUCUCACAACGGGCAU[dT][dT]-3’) or  the human ADAR2 targeting siRNA: ADAR2: (5’-GAUCGUGGCCUUGCAUUAA[dT][dT]-3’). Moreover, cells were stimulated with IL6 (10ng/ml)+sIL6Ralpha (100ng/ml) for 4h. All siRNAs were purchased and validated by Sigma Aldrich and our study.

value: human umbilical vein endothelial cells

value: passage 3

tag: treatment

value: siADAR2 + Il6 Stimulation


title: UF_KS_045_S9_L002_R1_001

value: Scrambled


title: UF_KS_046_S10_L002_R1_001

value: Scrambled + Il6 Stimulation


title: RNA-Seq_VascAN_Pt4

value: patient 4


title: RNA-Seq_HUVEC_Pt3

description:
control
Gene_Expression_RPKM.txt

source_name: Human umbilical vein endothelial cells

value: umbilical vein endothelial cells

value: replicate 3


title: RNA-Seq_HUVEC_Pt1

description:
control
Gene_Expression_RPKM.txt

value: replicate 1


title: RNA-Seq_VascAN_Pt1

value: patient 1


title: RNA-Seq_HUVEC_Pt2

description:
control
Gene_Expression_RPKM.txt


title: UF_KS_050_S14_L002_R1_001


title: RNA-Seq_HsaVEC_Pt3

description:
control
Gene_Expression_RPKM.txt


title: Input

data_processing:
First 3 nucleotides of the reads were trimmed and  reads filtered (>35 score) with FASTX toolkit (v0.0.13)
ChIP-Seq reads were mapped against the human genome (GRCh38, Ensembl release 77) with Bowtie (v1.0.1) using the following parameters: alignment mode –v; max mismatches 3; multiple alignments allowed 1; best reportable alignment (--best --strata).
Peak calling was performed with the HOMER suite (findPeaks.pl) using Input as control and the following parameters: transcription factor optimized peak enrichment (-style factor); Fold-over-Input ≥3.5; Poisson p-value over input ≤ 1.00e-04; FDR rate ≤0.001.
Genome_build: hg38
Supplementary_files_format_and_content: bedGraph files were generated with HOMER using default settings

source_name: Jurkat T-ALL cells

extract_protocol:
Chromatin from cross-linked cells (1% formaldehyde, 20 min) was sonicated, incubated overnight with the indicated antibodies in RIPA buffer, precipitated with protein G/A-Sepharose and cross-linkage of the co-precipitated DNA-protein complexes was reversed.
Libraries were prepared using the NEBNext® Ultra™ DNA Library Prep Kit for Illumina (New England Biolabs) and sequenced on the Solexa HiSeq 2000 sequencer (Illumina) according to manufacturer's instructions.

tag: cell line

value: Jurkat

tag: chip antibody


title: RNA-Seq_VascAN_Pt3

value: patient 3


title: UF_KS_038_S2_L001_R1_001


title: UF_KS_048_S12_L002_R1_001


title: 7901PS (7901 positive cells)

city: BEIJING

first: XJ

last: Cheng

state: BEIJING

address: 52 FUCHENG ROAD

institute: Beijing Cancer Hospital

data_processing:
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm8 whole genome using bowtie v0.12.2 with parameters -q -p 4 -e 100 -y -a -m 10 --best --strata
Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using a protocol from Chepelev et al., Nucleic Acids Research, 2009. In short, exons from all isoforms of a gene were merged to create one meta-transcript. The number of reads falling in the exons of this meta-transcript were counted and normalized by the size of the meta-transcript and by the size of the library.
Supplementary_files_format_and_content: tab-delimited text files include RPKM values for each Sample ...

source_name: 7901 positive cells

extract_protocol:
Cells were removed and sorted by FACS, then RNA was harvested using Trizol reagent. VAHTS total RNA-seq (H/M/R) library PrepKit for IIIumina (Vazyme, NR603-02) was used with 1 ug of total RNA for the construction of sequencing libraries.
Follow the experimental instructions to perform the steps of rRNA removal, fragmentation, first-strand cDNA synthesis, second-strand cDNA synthesis, end repair, 3 'end plus A, ligation, and enrichment on the purified Total RNA to complete the sequencing sample library Construct (related productions were VAHTS total RNA-seq (H/M/R) library PrepKit for IIIumina, Vazyme, NR603-02; Agencourt AMPure XP Beads, Beckman, A63881; SuperScript II Reverse Transcriptase, Invitrogen, 18064014; Qubit dsDNA HS Assay Kit, Invitrogen, Q32854; Agilent High Sensitivity DNA Kit, Agilent, 5067-4626).

value: Gastric cancer cells

tag: passages


title: 7901NG (7901 TfR1 negative cells)

source_name: 7901 TfR1 negative cells


title: UF_KS_037_S1_L001_R1_001


title: UF_KS_042_S6_L001_R1_001


title: RNA-Seq_HsaVEC_Pt1

description:
control
Gene_Expression_RPKM.txt


title: bCat_ChIPSeq

value: β-Catenin (BD Bioscience, 610154)


title: N1IC_ChIPSeq

description: antibody reference: Wang, H., Zou, J., Zhao, B., Johannsen, E., Ashworth, T., Wong, H., Pear, W.S., Schug, J., Blacklow, S.C., Arnett, K.L., et al. (2011). Genome-wide analysis reveals conserved and divergent features of Notch1/RBPJ binding in human and murine T-lymphoblastic leukemia cells. Proc Natl Acad Sci U S A 108, 14908-14913.

value: Notch-IC (provided by Dr. Jon C. Aster)


title: UF_KS_051_S15_L002_R1_001


title: UF_KS_040_S4_L001_R1_001


title: UF_KS_049_S13_L002_R1_001


title: UF_KS_041_S5_L001_R1_001


title: DNCB_HY209(+) 3

city: Hongcheon

first: Aziz

last: Ghaderpour

state: Gangwon-do

address: 101 Dabyeonbat-gil, Hwachon-myeon,

department: biomedical

country: South Korea

institute: Seoul national university

description: gene expression from mouse skin

hyb_protocol: RNA was amplified using a 3’ IVT PLUS reagent kit (Affymetrix, Santa Clara, CA, USA), hybridized onto a Mouse Gene 430A 2.0 Arrays (Affymetrix) using a Hybridization kit (Affymetrix).

scan_protocol: Arrays were scanned using GeneChip Scanner 3000 7G (Affymetrix, Mouse 430_2, genome version mm10)

data_processing: Array data were normalized using the Affymetrix expression console MAS 5.0 method as described previously. Statistical significance was calculated using Welch’s t-test with a non-corrected p-value<0.05 and validated using permutation testing across samples. Most significant probe sets were submitted to ingenuity pathway analysis (IPA) software (IPA®, QIAGEN, Redwood City) for pathway analysis

source_name: Skin DNCB and HY209 Treatment

label_protocol: RNA was amplified using a 3’ IVT PLUS reagent kit (Affymetrix, Santa Clara, CA, USA), labeled onto a Mouse Gene 430A 2.0 Arrays (Affymetrix) using a 3’IVT Terminal Labeling

growth_protocol: DNCB was applied on the dorsal skin of Balb/c mice for 15 days

extract_protocol: Total RNA was isolated and purified from frozen skin tissues using TRIzol according to the manufacturer's protocol (Ambion, Austin, TX, USA). The quality and concentration of RNA were determined using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific). RNA integrity was analyzed using an Agilent Bioanalyzer 2100.

treatment_protocol: HY209 treated with vehicle for the last 7 days

value: Skin


title: DNCB_HY209(-) 3

description: gene expression from mouse skin

source_name: skin only DNCB treatment


title: DNCB_HY209(+) 1

description: gene expression from mouse skin


title: DNCB_HY209(+) 2

description: gene expression from mouse skin


title: DNCB_HY209(-) 2

description: gene expression from mouse skin


title: Th9-polarized CD4 T-cells, pre-treated with S. aureus (donor 3)

city: Stockholm

first: Isabella

last: Badolati

address: Svante Arrhenius väg 20C

department: Department of Molecular Biosciences

country: Sweden

institute: Stockholm University

data_processing:
Basecalling and demultiplexing was performed using Illumina bcl2fastq (v2.20).
Reads were aligned to Ensembl GRCh38 reference genome using STAR (v2.6.1d).
Gene counts were estimated using featureCounts (v1.5.1).
Assembly: GRCh38
Supplementary files format and content: tab-delimited text file including matrix of raw counts

source_name: CD4 T-cells

growth_protocol: 500.000 isolated CD4 T-cells were cultured together with 100.000 autologous monocytes in cell-culture medium contiaining RPMI-1640 supplemented with 20 mM HEPES, 10% FCS, 100 U/mL penicillin, 100 ug/mL streptomycin and 2 mM L-glutamine, at 37°C and 5% CO2. Different treatments/stimulations were added depending on the sample.

extract_protocol:
RNA was isolated using the mirVana miRNA Isolation kit (Ambion by Life Technologies), following the protocol for total RNA extraction.
Library was prepared using the Illumina Stranded mRNA Prep Ligation protocol (Illumina), following manufacturer's instructions. The yield and quality of library was assessed using Qubit (Thermo Fisher Scientific) and the Agilent TapeStation.

treatment_protocol: Cells were either left untreated or treated for 24h with 2.5% S. aureus cell-free supernatant, 2 ug/mL all-trans retinoic acid or the combination of the two. After that, CD3:CD28 beads were added, alone or together with IL-4 and TGF-beta for 72h.

value: CD4 T-cells

value: treated with S.aureus-derived factors for 24h, then stimulated with CD3:CD28 beads plus IL-4 and TGF-beta for 72h


title: Th9-polarized CD4 T-cells, pre-treated with S. aureus (donor 4)


title: Th9-polarized CD4 T-cells, pre-treated with S. aureus and retinoic acid (donor 3)

value: treated with S. aureus-derived factors and retinoic acid for 24h, then stimulated with CD3:CD28 beads plus IL-4 and TGF-beta for 72h


title: Th9-polarized CD4 T-cells, pre-treated with S. aureus and retinoic acid (donor 4)


title: Th9-polarized CD4 T-cells, pre-treated with S. aureus and retinoic acid (donor 2)


title: Th9-polarized CD4 T-cells, pre-treated with retinoic acid (donor 3)

value: treated with retinoic acid for 24h, then stimulated with CD3:CD28 beads plus IL-4 and TGF-beta for 72h


title: CD4 T-cells, untreated (donor 4)

value: untreated


title: Th9-polarized CD4 T-cells, pre-treated with retinoic acid (donor 1)


title: Th9-polarized CD4 T-cells, pre-treated with retinoic acid (donor 4)


title: Th9-polarized CD4 T-cells, pre-treated with S. aureus and retinoic acid (donor 1)


title: Th9-polarized CD4 T-cells, untreated, control (donor 3)

value: untreated for 24h, then stimulated with CD3:CD28 beads plus IL-4 and TGF-beta for 72h


title: Activated CD4 T-cells, untreated (donor 4)

value: untreated for 24h, then stimulated with CD3:CD28 beads for 72h


title: Th9-polarized CD4 T-cells, untreated, control (donor 4)


title: Activated CD4 T-cells, untreated (donor 2)


title: Th9-polarized CD4 T-cells, untreated, control (donor 1)


title: Th9-polarized CD4 T-cells, pre-treated with retinoic acid (donor 2)


title: Activated CD4 T-cells, untreated (donor 1)


title: CD4 T-cells, untreated (donor 3)


title: Activated CD4 T-cells, untreated (donor 3)


title: Th9-polarized CD4 T-cells, untreated, control (donor 2)


title: Th9-polarized CD4 T-cells, pre-treated with S. aureus (donor 2)


title: Th9-polarized CD4 T-cells, pre-treated with S. aureus (donor 1)


title: Sham 2

description: gene expression from mouse skin

source_name: skin no DNCB no HY209 treatment


title: N3_1677_OX_LpC

city: Marburg

first: Wilhelm

last: Bertrams

address: Hans-Meerwein-Str. 2

department: Institute for Lung Research

country: Germany

institute: Philipps University Marburg

data_processing:
de-multiplexed fastq files were tested with FastQC. Over-represented sequences and adapters were trimmed with the Qiagen CLC Workbench Software v. 10.0.1.
Reads were mapped with the Qiagen CLC Workbench Software v. 10.0.1., using the hg19 human reference genome using default mapping parameters
Mapped total reads were analysed with the R package DeSeq2 v. 1.22.2 in R version 3.5.1, and the contrasts 1677_OX_vs_CTRL, 1677_OX_LpC_vs_CTRL_LpC and CTRL_LpC_vs_CTRL were calculated
The DeSeq2 output, namely the contrast mentioned, are provided as excel file processed_results.xlsx
Genome_build: hg19
Supplementary_files_format_and_content: read_count_data.xlsx. processed_results.xlsx, DeSeq2 contrasts 1677_OX_vs_CTRL, 1677_OX_LpC_vs_CTRL_LpC and CTRL_LpC_vs_CTRL.

growth_protocol: THP-1 cells were cultured with RPMI-1640 containing 10% FCS, 1% glutamax and 1% sodium pyruvate in an incubator with 37°C, 5% CO2 and humidified atmosphere

extract_protocol:
RNA was isolated by phenol-chloroform extraction with Trizol Reagent followed by DNaseI digestion.
RNA libraries were prepared for sequencing using standard Illumina protocols.

treatment_protocol: Cells were stimulated with 80 nM PMA for 72 hours and cells were infected with L.p. (MOI 1) for 3 hours.

value: LPC_1677 overexpression

tag: infection

value: Lp


title: DNCB_HY209(-) 1

description: gene expression from mouse skin


title: N3_1677_OX

value: control


title: N3_CTRL_LpC


title: Sham 1

description: gene expression from mouse skin


title: N1_1677_OX_LpC


title: N2_1677_OX


title: N2_CTRL


title: N2_1677_OX_LpC


title: N1_CTRL_LpC


title: N1_1677_OX


title: N1_CTRL


title: N2_CTRL_LpC


title: CD4 T-cells, untreated (donor 1)


title: 8.18.21 3 2.25mm 90s

city: Stanford

first: D.

middle: Blaine

last: Marchant

state: California

address: Gilbert Biological Sciences Building, 371 Serra Mall

department: Biology

institute: Stanford University

description: UMI Matrix

library_source: transcriptomic single cell

data_processing:
Multiplexed libraries were sequenced with paired-end sequencing. The first read of each read pair contained exclusively barcoding information: a 6 nt sample barcode and a 10 bp unique molecular identifier (UMI). Paired-end reads were demultiplexed based on the sample barcodes, requiring a perfect match to one of the barcode sequences. The UMIs were extracted and appended to the read 2 sequence identifiers. The submitted fastq files are demultiplexed files for read2, with the UMI from read 1 appended to the sequence identifiers.
Prior to alignment, reads were trimmed and filtered using Fastp with parameters -y -x -3 -f 6. Reads were then aligned to the AGPv4 genome assembly using HiSat2.
The number of UMIs for each gene and each sample were counted, allowing for a 1 bp mismatch in UMIs to avoid over-counting artifacts resulting from sequencing errors
Assembly: AgpV4
Supplementary files format and content: CSV containing the UMI counts for each cell (column) and gene (row)

organism: Zea mays

source_name: anther

growth_protocol: individuals were grown under greenhouse conditions in Stanford, CA, USA with 14-h day/10-h night lighting, approximating half of peak summer solar radiation including UV-A. Daily irrigation and fertilization maintained robust growth

extract_protocol:
Anthers dissected, fixed, digested, isolated using a Hana Cell Sorter (NamoCell) following FX-Cell protocol (Marchant et al. 2022)
CEL-Seq2 (Hashimshony et al., 2016) with modifications per Nelms and Walbot 2019.

value: anther

value: anther single cell


title: 7.21 5 0.75mm 20s

description: UMI Matrix


title: 8.18.21 2 1.25mm 5s

description: UMI Matrix


title: 7.21 2 2.0mm 22s

description: UMI Matrix


title: 3.29.22 1 1.25mm 60s

description: UMI Matrix


title: CD4 T-cells, untreated (donor 2)


title: 3.29.22 2 3.0mm 13s

description: UMI Matrix


title: 8.18.21 10 3.0mm 35s

description: UMI Matrix


title: 8.18.21 9 3.0mm 55s

description: UMI Matrix


title: 3.29.22 2 3.0mm 20s

description: UMI Matrix


title: 7.21 1 2.0mm 70s

description: UMI Matrix


title: 8.18.21 4 2.25mm 93s

description: UMI Matrix


title: 1.4.21 4 3.0mm 8s

description: UMI Matrix


title: 8.18.21 9 3.0mm 3s

description: UMI Matrix


title: 8.18.21 4 2.25mm 76s

description: UMI Matrix


title: 1.4.21 4 3.0mm 94s

description: UMI Matrix


title: 3.29.22 2 3.0mm 48s

description: UMI Matrix


title: N3_CTRL


title: 1.4.21 4 3.0mm 14s

description: UMI Matrix


title: 3.29.22 1 1.25mm 7s

description: UMI Matrix


title: 3.29.22 1 3.0mm 96s

description: UMI Matrix


title: 3.29.22 1 1.25mm 53s

description: UMI Matrix


title: 1.4.21 1 1.25mm 14s

description: UMI Matrix


title: 8.18.21 3 2.25mm 44s

description: UMI Matrix


title: 8.18.21 3 2.25mm 66s

description: UMI Matrix


title: 3.29.22 2 3.0mm 43s

description: UMI Matrix


title: 1.4.21 4 2.5mm 58s

description: UMI Matrix


title: 8.18.21 9 3.0mm 19s

description: UMI Matrix


title: 7.21 2 2.0mm 67s

description: UMI Matrix


title: 3.29.22 1 2.25mm 73s

description: UMI Matrix


title: 8.18.21 10 3.0mm 20s

description: UMI Matrix


title: 8.18.21 10 3.0mm 87s

description: UMI Matrix


title: 8.18.21 1 2.5mm 83s

description: UMI Matrix


title: 3.29.22 2 3.0mm 31s

description: UMI Matrix


title: 8.18.21 1 1.75mm 94s

description: UMI Matrix


title: 7.21 1 2.0mm 84s

description: UMI Matrix


title: 3.29.22 2 3.0mm 52s

description: UMI Matrix


title: 3.29.22 2 3.0mm 3s

description: UMI Matrix


title: 3.29.22 2 3.0mm 83s

description: UMI Matrix


title: 8.18.21 3 2.25mm 94s

description: UMI Matrix


title: 8.18.21 8 0.5mm 84s

description: UMI Matrix


title: 1.4.21 1 1.0mm 35s

description: UMI Matrix


title: 8.18.21 3 2.75mm 24s

description: UMI Matrix


title: 3.29.22 2 3.0mm 25s

description: UMI Matrix


title: 8.18.21 3 2.25mm 40s

description: UMI Matrix


title: 3.29.22 1 1.25mm 44s

description: UMI Matrix


title: 3.29.22 2 3.0mm 67s

description: UMI Matrix


title: 3.29.22 1 3.0mm 52s

description: UMI Matrix


title: 3.29.22 2 3.0mm 30s

description: UMI Matrix


title: 1.4.21 4 3.0mm 88s

description: UMI Matrix


title: 1.4.21 4 3.0mm 78s

description: UMI Matrix


title: 8.18.21 10 3.0mm 5s

description: UMI Matrix


title: 1.4.21 2 2.25mm 59s

description: UMI Matrix


title: 3.29.22 1 3.0mm 51s

description: UMI Matrix


title: 1.4.21 4 3.0mm 9s

description: UMI Matrix


title: 3.29.22 2 3.0mm 68s

description: UMI Matrix


title: 1.4.21 4 2.5mm 87s

description: UMI Matrix


title: 1.4.21 4 3.0mm 13s

description: UMI Matrix


title: 8.18.21 3 2.25mm 48s

description: UMI Matrix


title: 1.4.21 1 0.75mm 14s

description: UMI Matrix


title: 1.4.21 4 3.0mm 15s

description: UMI Matrix


title: 8.18.21 3 2.75mm 29s

description: UMI Matrix


title: 7.21 1 2.0mm 36s

description: UMI Matrix


title: 1.4.21 4 3.0mm 3s

description: UMI Matrix


title: 3.29.22 2 3.0mm 15s

description: UMI Matrix


title: 3.29.22 2 3.0mm 8s

description: UMI Matrix


title: 1.4.21 1 1.25mm 2s

description: UMI Matrix


title: 3.29.22 1 3.0mm 29s

description: UMI Matrix


title: 3.29.22 2 3.0mm 51s

description: UMI Matrix


title: 3.29.22 2 3.0mm 41s

description: UMI Matrix


title: 8.18.21 8 0.5mm 68s

description: UMI Matrix


title: 1.4.21 3 0.75mm 38s

description: UMI Matrix


title: 3.29.22 2 3.0mm 32s

description: UMI Matrix


title: 3.29.22 2 3.0mm 45s

description: UMI Matrix


title: 8.18.21 2 1.0mm 60s

description: UMI Matrix


title: 8.18.21 2 1.75mm 66s

description: UMI Matrix


title: 8.18.21 3 2.75mm 42s

description: UMI Matrix


title: 3.29.22 1 1.25mm 75s

description: UMI Matrix


title: 1.4.21 1 1.25mm 46s

description: UMI Matrix


title: 7.21 2 2.0mm 66s

description: UMI Matrix


title: 7.21 5 0.75mm 8s

description: UMI Matrix


title: 8.18.21 10 3.0mm 1s

description: UMI Matrix


title: 1.4.21 4 2.5mm 29s

description: UMI Matrix


title: 1.4.21 4 2.5mm 59s

description: UMI Matrix


title: 3.29.22 1 3.0mm 42s

description: UMI Matrix


title: 3.29.22 1 2.75mm 34s

description: UMI Matrix


title: 1.4.21 4 2.5mm 28s

description: UMI Matrix


title: 3.29.22 1 1.25mm 43s

description: UMI Matrix


title: 8.18.21 3 2.75mm 7s

description: UMI Matrix


title: 3.29.22 1 2.75mm 20s

description: UMI Matrix


title: 1.4.21 3 0.75mm 60s

description: UMI Matrix


title: 1.4.21 1 1.25mm 75s

description: UMI Matrix


title: 3.29.22 2 3.0mm 14s

description: UMI Matrix


title: 3.29.22 2 3.0mm 37s

description: UMI Matrix


title: 3.29.22 2 3.0mm 63s

description: UMI Matrix


title: 3.29.22 2 3.0mm 47s

description: UMI Matrix


title: 8.18.21 2 1.25mm 85s

description: UMI Matrix


title: 3.29.22 1 1.25mm 76s

description: UMI Matrix


title: 1.4.21 2 2.0mm 75s

description: UMI Matrix


title: 3.29.22 2 3.0mm 46s

description: UMI Matrix


title: 3.29.22 2 3.0mm 35s

description: UMI Matrix


title: 8.18.21 2 1.75mm 47s

description: UMI Matrix


title: 3.29.22 1 1.25mm 9s

description: UMI Matrix


title: 8.18.21 2 1.25mm 70s

description: UMI Matrix


title: 3.29.22 2 3.0mm 23s

description: UMI Matrix


title: 3.29.22 1 3.0mm 55s

description: UMI Matrix


title: 8.18.21 2 1.75mm 70s

description: UMI Matrix


title: 8.18.21 2 1.75mm 19s

description: UMI Matrix


title: 3.29.22 2 3.0mm 19s

description: UMI Matrix


title: 8.18.21 4 2.25mm 42s

description: UMI Matrix


title: 8.18.21 2 1.25mm 45s

description: UMI Matrix


title: 1.4.21 4 3.0mm 55s

description: UMI Matrix


title: 8.18.21 2 1.75mm 43s

description: UMI Matrix


title: 1.4.21 2.5 2.0mm 26s

description: UMI Matrix


title: 1.4.21 3 1.0mm 66s

description: UMI Matrix


title: 3.29.22 2 3.0mm 84s

description: UMI Matrix


title: 8.18.21 2 1.25mm 91s

description: UMI Matrix


title: 3.29.22 2 3.0mm 24s

description: UMI Matrix


title: 3.29.22 2 3.0mm 10s

description: UMI Matrix


title: 3.29.22 1 1.5mm 94s

description: UMI Matrix


title: 3.29.22 1 3.0mm 16s

description: UMI Matrix


title: 1.4.21 2 2.25mm 76s

description: UMI Matrix


title: 3.29.22 2 3.0mm 12s

description: UMI Matrix


title: 1.4.21 2 2.25mm 22s

description: UMI Matrix


title: 3.29.22 1 1.25mm 12s

description: UMI Matrix


title: 1.4.21 1.3 2.0mm 10s

description: UMI Matrix


title: 8.18.21 3 2.25mm 1s

description: UMI Matrix


title: 3.29.22 1 3.0mm 31s

description: UMI Matrix


title: 3.29.22 2 3.0mm 22s

description: UMI Matrix


title: 1.4.21 4 3.0mm 37s

description: UMI Matrix


title: 3.29.22 1 3.0mm 82s

description: UMI Matrix


title: 1.4.21 4 3.0mm 63s

description: UMI Matrix


title: 3.29.22 2 3.0mm 4s

description: UMI Matrix


title: 8.18.21 3 2.25mm 35s

description: UMI Matrix


title: 8.18.21 3 2.75mm 92s

description: UMI Matrix


title: 8.18.21 2 1.25mm 38s

description: UMI Matrix


title: 8.18.21 2 1.75mm 5s

description: UMI Matrix


title: 3.29.22 1 2.75mm 26s

description: UMI Matrix


title: 7.21 1 2.0mm 42s

description: UMI Matrix


title: 8.18.21 1 2.5mm 49s

description: UMI Matrix


title: 1.4.21 1.3 2.0mm 26s

description: UMI Matrix


title: 8.18.21 3 2.75mm 33s

description: UMI Matrix


title: 8.18.21 2 1.25mm 31s

description: UMI Matrix


title: 8.18.21 2 1.25mm 37s

description: UMI Matrix


title: 8.18.21 3 2.75mm 17s

description: UMI Matrix


title: 7.21 1 2.0mm 19s

description: UMI Matrix


title: 3.29.22 2 3.0mm 36s

description: UMI Matrix


title: 7.21 1 2.0mm 68s

description: UMI Matrix


title: 1.4.21 4 2.5mm 32s

description: UMI Matrix


title: 1.4.21 4 3.0mm 61s

description: UMI Matrix


title: 3.29.22 1 2.75mm 15s

description: UMI Matrix


title: 7.21 1 2.0mm 87s

description: UMI Matrix


title: 3.29.22 1 2.75mm 55s

description: UMI Matrix


title: 8.18.21 2 1.75mm 46s

description: UMI Matrix


title: 7.21 5 0.75mm 40s

description: UMI Matrix


title: 3.29.22 1 1.25mm 17s

description: UMI Matrix


title: 8.18.21 2 1.25mm 10s

description: UMI Matrix


title: 1.4.21 1 1.0mm 7s

description: UMI Matrix


title: 8.18.21 3 2.25mm 57s

description: UMI Matrix


title: 8.18.21 1 1.75mm 46s

description: UMI Matrix


title: 3.29.22 1 2.75mm 92s

description: UMI Matrix


title: 1.4.21 4 2.5mm 63s

description: UMI Matrix


title: 8.18.21 2 1.75mm 45s

description: UMI Matrix


title: 1.4.21 2 2.5mm 38s

description: UMI Matrix


title: 8.18.21 2 1.75mm 6s

description: UMI Matrix


title: 8.18.21 2 1.25mm 47s

description: UMI Matrix


title: 8.18.21 2 1.25mm 61s

description: UMI Matrix


title: 8.18.21 3 2.25mm 51s

description: UMI Matrix


title: 1.4.21 2 2.75mm 66s

description: UMI Matrix


title: 8.18.21 5 1.25mm 51s

description: UMI Matrix


title: 1.4.21 2.2 2.0mm 9s

description: UMI Matrix


title: 8.18.21 2 1.75mm 25s

description: UMI Matrix


title: 3.29.22 1 2.75mm 9s

description: UMI Matrix


title: 8.18.21 3 2.25mm 27s

description: UMI Matrix


title: 3.29.22 1 1.25mm 45s

description: UMI Matrix


title: 1.4.21 6 1.5mm 2s

description: UMI Matrix


title: 3.29.22 1 1.5mm 13s

description: UMI Matrix


title: 3.29.22 1 2.75mm 71s

description: UMI Matrix


title: 1.4.21 1 1.25mm 64s

description: UMI Matrix


title: 3.29.22 1 2.75mm 53s

description: UMI Matrix


title: 8.18.21 3 2.75mm 83s

description: UMI Matrix


title: 8.18.21 3 2.75mm 63s

description: UMI Matrix


title: 8.18.21 2 1.5mm 47s

description: UMI Matrix


title: 1.4.21 4 2.5mm 26s

description: UMI Matrix


title: 8.18.21 3 2.75mm 30s

description: UMI Matrix


title: 8.18.21 2 1.5mm 46s

description: UMI Matrix


title: 7.21 2 2.0mm 11s

description: UMI Matrix


title: 8.18.21 3 2.75mm 27s

description: UMI Matrix


title: 8.18.21 2 1.75mm 32s

description: UMI Matrix


title: 8.18.21 3 2.75mm 31s

description: UMI Matrix


title: 3.29.22 1 2.25mm 8s

description: UMI Matrix


title: 8.18.21 3 2.75mm 50s

description: UMI Matrix


title: 3.29.22 1 1.25mm 50s

description: UMI Matrix


title: 8.18.21 2 1.75mm 31s

description: UMI Matrix


title: 3.29.22 1 2.75mm 51s

description: UMI Matrix


title: 1.4.21 1 0.75mm 75s

description: UMI Matrix


title: 1.4.21 4 3.0mm 28s

description: UMI Matrix


title: 8.18.21 10 3.0mm 46s

description: UMI Matrix


title: 3.29.22 1 2.75mm 72s

description: UMI Matrix


title: 3.29.22 1 3.0mm 13s

description: UMI Matrix


title: 1.4.21 3 0.75mm 29s

description: UMI Matrix


title: 8.18.21 2 1.5mm 20s

description: UMI Matrix


title: 8.18.21 2 1.25mm 59s

description: UMI Matrix


title: 3.29.22 1 1.25mm 85s

description: UMI Matrix


title: 8.18.21 3 2.25mm 14s

description: UMI Matrix


title: 8.18.21 6 2.75mm 7s

description: UMI Matrix


title: 3.29.22 1 2.75mm 8s

description: UMI Matrix


title: 8.18.21 5 1.25mm 69s

description: UMI Matrix


title: 3.29.22 1 2.75mm 42s

description: UMI Matrix


title: 8.18.21 5 1.25mm 70s

description: UMI Matrix


title: 8.18.21 2 1.25mm 60s

description: UMI Matrix


title: 3.29.22 1 2.75mm 13s

description: UMI Matrix


title: 8.18.21 3 2.75mm 25s

description: UMI Matrix


title: 1.4.21 2 2.0mm 24s

description: UMI Matrix


title: 1.4.21 2 2.75mm 58s

description: UMI Matrix


title: 3.29.22 1 2.75mm 17s

description: UMI Matrix


title: 3.29.22 1 2.75mm 33s

description: UMI Matrix


title: 8.18.21 1 1.5mm 38s

description: UMI Matrix


title: 8.18.21 3 2.75mm 28s

description: UMI Matrix


title: 3.29.22 1 2.75mm 1s

description: UMI Matrix


title: 3.29.22 1 1.25mm 59s

description: UMI Matrix


title: 3.29.22 1 3.0mm 34s

description: UMI Matrix


title: 7.21 2 2.0mm 61s

description: UMI Matrix


title: 8.18.21 4 2.25mm 81s

description: UMI Matrix


title: 3.29.22 1 2.75mm 37s

description: UMI Matrix


title: 8.18.21 3 2.5mm 28s

description: UMI Matrix


title: 8.18.21 6 2.75mm 59s

description: UMI Matrix


title: 8.18.21 1 1.75mm 85s

description: UMI Matrix


title: 8.18.21 1 2.5mm 68s

description: UMI Matrix


title: 8.18.21 5 1.25mm 48s

description: UMI Matrix


title: 1.4.21 2 2.0mm 18s

description: UMI Matrix


title: 1.4.21 3 0.5mm 30s

description: UMI Matrix


title: 8.18.21 6 2.75mm 19s

description: UMI Matrix


title: 8.18.21 2 1.25mm 25s

description: UMI Matrix


title: 3.29.22 1 1.5mm 19s

description: UMI Matrix


title: 8.18.21 2 1.0mm 75s

description: UMI Matrix


title: 3.29.22 1 2.75mm 84s

description: UMI Matrix


title: 8.18.21 6 2.75mm 51s

description: UMI Matrix


title: 3.29.22 1 2.75mm 88s

description: UMI Matrix


title: 3.29.22 1 1.25mm 28s

description: UMI Matrix


title: 7.21 2 2.0mm 2s

description: UMI Matrix


title: 8.18.21 5 1.25mm 37s

description: UMI Matrix


title: 3.29.22 1 1.5mm 8s

description: UMI Matrix


title: 1.4.21 1 1.25mm 61s

description: UMI Matrix


title: 8.18.21 8 0.5mm 35s

description: UMI Matrix


title: 3.29.22 1 2.75mm 96s

description: UMI Matrix


title: 1.4.21 4 2.5mm 10s

description: UMI Matrix


title: 1.4.21 1 1.25mm 28s

description: UMI Matrix


title: 8.18.21 3 2.75mm 51s

description: UMI Matrix


title: 8.18.21 5 1.25mm 19s

description: UMI Matrix


title: 8.18.21 3 2.25mm 34s

description: UMI Matrix


title: 1.4.21 4 2.5mm 7s

description: UMI Matrix


title: 3.29.22 1 2.75mm 23s

description: UMI Matrix


title: 8.18.21 2 1.0mm 96s

description: UMI Matrix


title: 1.4.21 1 1.25mm 23s

description: UMI Matrix


title: 8.18.21 3 2.75mm 62s

description: UMI Matrix


title: 1.4.21 4 3.0mm 76s

description: UMI Matrix


title: 3.29.22 2 3.0mm 71s

description: UMI Matrix


title: 1.4.21 1 1.25mm 26s

description: UMI Matrix


title: 1.4.21 4 2.5mm 62s

description: UMI Matrix


title: 1.4.21 4 3.0mm 60s

description: UMI Matrix


title: 8.18.21 2 1.25mm 46s

description: UMI Matrix


title: 8.18.21 2 1.25mm 51s

description: UMI Matrix


title: 3.29.22 1 1.25mm 11s

description: UMI Matrix


title: 1.4.21 1 1.25mm 1s

description: UMI Matrix


title: 8.18.21 4 2.25mm 63s

description: UMI Matrix


title: 8.18.21 5 1.25mm 11s

description: UMI Matrix


title: 8.18.21 2 1.25mm 2s

description: UMI Matrix


title: 8.18.21 3 2.25mm 49s

description: UMI Matrix


title: 1.4.21 1.3 2.0mm 37s

description: UMI Matrix


title: 1.4.21 1 1.25mm 39s

description: UMI Matrix


title: 8.18.21 4 2.25mm 80s

description: UMI Matrix


title: 1.4.21 1 1.0mm 46s

description: UMI Matrix


title: 8.18.21 3 2.75mm 5s

description: UMI Matrix


title: 3.29.22 1 1.5mm 7s

description: UMI Matrix


title: 3.29.22 1 1.25mm 82s

description: UMI Matrix


title: 8.18.21 1 2.5mm 85s

description: UMI Matrix


title: 1.4.21 1 1.25mm 5s

description: UMI Matrix


title: 8.18.21 5 1.25mm 45s

description: UMI Matrix


title: 8.18.21 2 1.25mm 22s

description: UMI Matrix


title: 1.4.21 1 1.25mm 34s

description: UMI Matrix


title: 1.4.21 2.2 2.0mm 5s

description: UMI Matrix


title: 1.4.21 1 1.25mm 41s

description: UMI Matrix


title: 7.21 2 2.0mm 96s

description: UMI Matrix


title: 8.18.21 1 2.5mm 24s

description: UMI Matrix


title: 1.4.21 2 2.5mm 10s

description: UMI Matrix


title: 8.18.21 3 2.5mm 11s

description: UMI Matrix


title: 8.18.21 3 2.5mm 42s

description: UMI Matrix


title: 8.18.21 2 1.25mm 50s

description: UMI Matrix


title: 7.21 1 2.0mm 33s

description: UMI Matrix


title: 3.29.22 1 1.25mm 23s

description: UMI Matrix


title: 3.29.22 1 1.25mm 37s

description: UMI Matrix


title: 8.18.21 2 1.25mm 96s

description: UMI Matrix


title: 1.4.21 2 2.5mm 63s

description: UMI Matrix


title: 1.4.21 4 3.0mm 82s

description: UMI Matrix


title: 7.21 2 2.0mm 39s

description: UMI Matrix


title: 8.18.21 1 2.5mm 33s

description: UMI Matrix


title: 8.18.21 1 2.5mm 21s

description: UMI Matrix


title: 1.4.21 1 1.0mm 1s

description: UMI Matrix


title: 8.18.21 1 2.5mm 59s

description: UMI Matrix


title: 1.4.21 1 1.25mm 15s

description: UMI Matrix


title: 1.4.21 4 3.0mm 36s

description: UMI Matrix


title: 7.21 2 2.0mm 51s

description: UMI Matrix


title: 8.18.21 2 1.25mm 82s

description: UMI Matrix


title: 3.29.22 1 2.25mm 27s

description: UMI Matrix


title: 1.4.21 1 1.25mm 19s

description: UMI Matrix


title: 8.18.21 2 1.25mm 80s

description: UMI Matrix


title: 1.4.21 1 1.25mm 74s

description: UMI Matrix


title: 1.4.21 4 2.5mm 30s

description: UMI Matrix


title: 8.18.21 1 2.5mm 28s

description: UMI Matrix


title: 8.18.21 3 2.75mm 11s

description: UMI Matrix


title: 7.21 5 0.75mm 82s

description: UMI Matrix


title: 8.18.21 5 1.25mm 22s

description: UMI Matrix


title: 8.18.21 1 2.5mm 76s

description: UMI Matrix


title: 8.18.21 2 1.25mm 15s

description: UMI Matrix


title: 8.18.21 2 1.25mm 9s

description: UMI Matrix


title: 1.4.21 3 1.0mm 84s

description: UMI Matrix


title: 8.18.21 3 2.25mm 38s

description: UMI Matrix


title: 8.18.21 5 1.25mm 23s

description: UMI Matrix


title: 8.18.21 5 1.25mm 55s

description: UMI Matrix


title: 3.29.22 1 2.25mm 56s

description: UMI Matrix


title: 3.29.22 1 1.25mm 31s

description: UMI Matrix


title: 8.18.21 5 1.25mm 10s

description: UMI Matrix


title: 8.18.21 3 2.75mm 78s

description: UMI Matrix


title: 1.4.21 4 2.5mm 41s

description: UMI Matrix


title: 3.29.22 1 1.25mm 2s

description: UMI Matrix


title: 1.4.21 1 1.0mm 18s

description: UMI Matrix


title: 1.4.21 1 1.0mm 6s

description: UMI Matrix


title: 8.18.21 1 2.5mm 35s

description: UMI Matrix


title: 1.4.21 3 0.75mm 94s

description: UMI Matrix


title: 1.4.21 2 2.5mm 60s

description: UMI Matrix


title: 8.18.21 2 1.25mm 23s

description: UMI Matrix


title: 3.29.22 1 1.5mm 29s

description: UMI Matrix


title: 1.4.21 4 2.5mm 22s

description: UMI Matrix


title: 8.18.21 2 1.0mm 29s

description: UMI Matrix


title: 1.4.21 1 1.0mm 16s

description: UMI Matrix


title: 3.29.22 1 2.75mm 19s

description: UMI Matrix


title: 1.4.21 3 1.0mm 33s

description: UMI Matrix


title: 8.18.21 1 2.5mm 13s

description: UMI Matrix


title: 8.18.21 1 2.5mm 27s

description: UMI Matrix


title: 8.18.21 3 2.25mm 10s

description: UMI Matrix


title: 7.21 1 2.0mm 91s

description: UMI Matrix


title: 8.18.21 5 1.25mm 53s

description: UMI Matrix


title: 3.29.22 1 2.75mm 91s

description: UMI Matrix


title: 7.21 2 2.0mm 29s

description: UMI Matrix


title: 3.29.22 1 1.25mm 19s

description: UMI Matrix


title: 3.29.22 1 3.0mm 36s

description: UMI Matrix


title: 1.4.21 1 1.0mm 19s

description: UMI Matrix


title: 8.18.21 1 2.5mm 2s

description: UMI Matrix


title: 1.4.21 4 2.5mm 23s

description: UMI Matrix


title: 7.21 5 0.75mm 4s

description: UMI Matrix


title: 8.18.21 2 1.25mm 28s

description: UMI Matrix


title: 8.18.21 1 2.5mm 81s

description: UMI Matrix


title: 1.4.21 1 1.25mm 11s

description: UMI Matrix


title: 8.18.21 3 2.75mm 87s

description: UMI Matrix


title: 1.4.21 1.3 2.0mm 63s

description: UMI Matrix


title: 8.18.21 3 2.75mm 60s

description: UMI Matrix


title: 8.18.21 3 2.75mm 18s

description: UMI Matrix


title: 8.18.21 1 2.5mm 75s

description: UMI Matrix


title: 8.18.21 5 1.0mm 56s

description: UMI Matrix


title: 3.29.22 1 2.75mm 61s

description: UMI Matrix


title: 1.4.21 4 3.0mm 59s

description: UMI Matrix


title: 7.21 1 2.0mm 34s

description: UMI Matrix


title: 8.18.21 1 2.5mm 44s

description: UMI Matrix


title: 1.4.21 3 0.5mm 89s

description: UMI Matrix


title: 8.18.21 5 1.25mm 47s

description: UMI Matrix


title: 7.21 5 0.75mm 27s

description: UMI Matrix


title: 1.4.21 7 1.25mm 51s

description: UMI Matrix


title: 3.29.22 1 1.25mm 66s

description: UMI Matrix


title: 1.4.21 3 1.0mm 65s

description: UMI Matrix


title: 8.18.21 1 2.5mm 77s

description: UMI Matrix


title: 8.18.21 3 2.5mm 57s

description: UMI Matrix


title: 1.4.21 1 0.75mm 21s

description: UMI Matrix


title: 8.18.21 5 1.0mm 84s

description: UMI Matrix


title: 8.18.21 1 2.5mm 19s

description: UMI Matrix


title: 1.4.21 2 1.75mm 18s

description: UMI Matrix


title: 8.18.21 3 2.25mm 22s

description: UMI Matrix


title: 8.18.21 8 0.5mm 87s

description: UMI Matrix


title: 3.29.22 1 2.75mm 21s

description: UMI Matrix


title: 7.21 1 2.0mm 44s

description: UMI Matrix


title: 8.18.21 2 1.25mm 21s

description: UMI Matrix


title: 3.29.22 1 2.75mm 2s

description: UMI Matrix


title: 8.18.21 5 1.0mm 40s

description: UMI Matrix


title: 8.18.21 2 1.25mm 20s

description: UMI Matrix


title: 8.18.21 3 2.5mm 8s

description: UMI Matrix


title: 8.18.21 5 1.25mm 6s

description: UMI Matrix


title: 8.18.21 1 2.5mm 22s

description: UMI Matrix


title: 8.18.21 5 1.0mm 25s

description: UMI Matrix


title: 8.18.21 2 1.0mm 39s

description: UMI Matrix


title: 7.21 1 2.0mm 2s

description: UMI Matrix


title: 1.4.21 2 1.75mm 36s

description: UMI Matrix


title: 8.18.21 5 1.0mm 48s

description: UMI Matrix


title: 1.4.21 2 2.0mm 8s

description: UMI Matrix


title: 3.29.22 1 1.5mm 64s

description: UMI Matrix


title: 8.18.21 5 1.25mm 13s

description: UMI Matrix


title: 8.18.21 3 2.75mm 4s

description: UMI Matrix


title: 1.4.21 2 2.0mm 21s

description: UMI Matrix


title: 8.18.21 1 2.5mm 12s

description: UMI Matrix


title: 8.18.21 3 2.5mm 50s

description: UMI Matrix


title: 8.18.21 1 2.5mm 91s

description: UMI Matrix


title: 1.4.21 1 1.0mm 33s

description: UMI Matrix


title: 1.4.21 1 1.0mm 81s

description: UMI Matrix


title: 1.4.21 1 1.25mm 29s

description: UMI Matrix


title: 3.29.22 1 1.25mm 15s

description: UMI Matrix


title: 8.18.21 3 2.25mm 47s

description: UMI Matrix


title: 8.18.21 5 1.0mm 89s

description: UMI Matrix


title: 8.18.21 3 2.75mm 65s

description: UMI Matrix


title: 3.29.22 1 2.75mm 79s

description: UMI Matrix


title: 8.18.21 1 2.5mm 84s

description: UMI Matrix


title: 8.18.21 3 2.5mm 23s

description: UMI Matrix


title: 8.18.21 1 2.5mm 87s

description: UMI Matrix


title: 8.18.21 5 1.25mm 43s

description: UMI Matrix


title: 8.18.21 4 2.25mm 70s

description: UMI Matrix


title: 8.18.21 3 2.5mm 64s

description: UMI Matrix


title: 8.18.21 5 1.25mm 60s

description: UMI Matrix


title: 7.21 2 2.0mm 69s

description: UMI Matrix


title: 8.18.21 2 1.25mm 57s

description: UMI Matrix


title: 8.18.21 1 2.5mm 4s

description: UMI Matrix


title: 8.18.21 1 2.5mm 43s

description: UMI Matrix


title: 8.18.21 1 2.5mm 15s

description: UMI Matrix


title: 3.29.22 1 2.75mm 82s

description: UMI Matrix


title: 8.18.21 2 1.75mm 35s

description: UMI Matrix


title: 8.18.21 8 0.5mm 10s

description: UMI Matrix


title: 8.18.21 3 2.5mm 30s

description: UMI Matrix


title: 1.4.21 1 0.5mm 18s

description: UMI Matrix


title: 3.29.22 1 3.0mm 38s

description: UMI Matrix


title: 8.18.21 1 2.5mm 50s

description: UMI Matrix


title: 8.18.21 1 2.5mm 78s

description: UMI Matrix


title: 8.18.21 3 2.25mm 21s

description: UMI Matrix


title: 8.18.21 3 2.75mm 55s

description: UMI Matrix


title: 8.18.21 5 1.25mm 46s

description: UMI Matrix


title: 8.18.21 1 2.5mm 30s

description: UMI Matrix


title: 8.18.21 5 1.0mm 73s

description: UMI Matrix


title: 7.21 1 2.0mm 47s

description: UMI Matrix


title: 8.18.21 5 1.0mm 64s

description: UMI Matrix


title: 8.18.21 3 2.25mm 61s

description: UMI Matrix


title: 8.18.21 3 2.25mm 73s

description: UMI Matrix


title: 8.18.21 3 2.25mm 83s

description: UMI Matrix


title: 8.18.21 3 2.25mm 39s

description: UMI Matrix


title: 3.29.22 1 2.75mm 5s

description: UMI Matrix


title: 8.18.21 10 3.0mm 91s

description: UMI Matrix


title: 8.18.21 3 2.25mm 5s

description: UMI Matrix


title: 8.18.21 1 2.5mm 54s

description: UMI Matrix


title: 8.18.21 5 1.0mm 1s

description: UMI Matrix


title: 3.29.22 2 3.0mm 58s

description: UMI Matrix


title: 8.18.21 5 1.25mm 71s

description: UMI Matrix


title: 8.18.21 5 1.25mm 31s

description: UMI Matrix


title: 8.18.21 5 1.0mm 13s

description: UMI Matrix


title: 7.21 5 0.75mm 39s

description: UMI Matrix


title: 7.21 1 2.0mm 58s

description: UMI Matrix


title: 8.18.21 1 2.5mm 60s

description: UMI Matrix


title: 3.29.22 1 1.25mm 32s

description: UMI Matrix


title: 3.29.22 1 2.75mm 31s

description: UMI Matrix


title: 1.4.21 1 0.75mm 83s

description: UMI Matrix


title: 1.4.21 4 3.0mm 64s

description: UMI Matrix


title: 8.18.21 3 2.25mm 50s

description: UMI Matrix


title: 8.18.21 5 1.25mm 62s

description: UMI Matrix


title: 7.21 1 2.0mm 5s

description: UMI Matrix


title: 1.4.21 3 1.0mm 78s

description: UMI Matrix


title: 8.18.21 3 2.25mm 33s

description: UMI Matrix


title: 8.18.21 2 1.0mm 34s

description: UMI Matrix


title: 1.4.21 2 2.25mm 31s

description: UMI Matrix


title: 8.18.21 2 1.0mm 36s

description: UMI Matrix


title: 7.21 5 0.75mm 59s

description: UMI Matrix


title: 1.4.21 2 2.25mm 61s

description: UMI Matrix


title: 8.18.21 2 1.25mm 29s

description: UMI Matrix


title: 8.18.21 4 2.25mm 66s

description: UMI Matrix


title: 8.18.21 5 1.0mm 16s

description: UMI Matrix


title: 3.29.22 1 2.75mm 63s

description: UMI Matrix


title: 8.18.21 3 2.25mm 81s

description: UMI Matrix


title: 8.18.21 2 1.0mm 59s

description: UMI Matrix


title: 8.18.21 2 1.0mm 48s

description: UMI Matrix


title: 8.18.21 3 2.25mm 55s

description: UMI Matrix


title: 8.18.21 3 2.5mm 1s

description: UMI Matrix


title: 8.18.21 3 2.75mm 68s

description: UMI Matrix


title: 8.18.21 3 2.25mm 2s

description: UMI Matrix


title: 8.18.21 3 2.25mm 15s

description: UMI Matrix


title: 8.18.21 2 1.75mm 49s

description: UMI Matrix


title: 8.18.21 3 2.25mm 63s

description: UMI Matrix


title: 8.18.21 2 1.25mm 75s

description: UMI Matrix


title: 1.4.21 3 0.75mm 66s

description: UMI Matrix


title: 1.4.21 6 1.5mm 1s

description: UMI Matrix


title: 8.18.21 3 2.75mm 64s

description: UMI Matrix


title: 8.18.21 10 3.0mm 13s

description: UMI Matrix


title: 8.18.21 5 1.0mm 77s

description: UMI Matrix


title: 1.4.21 2 1.75mm 61s

description: UMI Matrix


title: 1.4.21 6 1.5mm 48s

description: UMI Matrix


title: 8.18.21 3 2.25mm 92s

description: UMI Matrix


title: 8.18.21 3 2.75mm 59s

description: UMI Matrix


title: 8.18.21 1 1.75mm 12s

description: UMI Matrix


title: 8.18.21 3 2.25mm 9s

description: UMI Matrix


title: 8.18.21 4 2.25mm 14s

description: UMI Matrix


title: 3.29.22 1 1.25mm 30s

description: UMI Matrix


title: 1.4.21 1.3 2.0mm 22s

description: UMI Matrix


title: 1.4.21 1 1.0mm 32s

description: UMI Matrix


title: 8.18.21 1 2.5mm 3s

description: UMI Matrix


title: 8.18.21 4 2.25mm 3s

description: UMI Matrix


title: 3.29.22 1 2.25mm 25s

description: UMI Matrix


title: 8.18.21 10 3.0mm 47s

description: UMI Matrix


title: 8.18.21 4 2.25mm 19s

description: UMI Matrix


title: 8.18.21 5 1.25mm 54s

description: UMI Matrix


title: 1.4.21 2 1.75mm 19s

description: UMI Matrix


title: 8.18.21 10 3.0mm 60s

description: UMI Matrix


title: 1.4.21 1 1.25mm 27s

description: UMI Matrix


title: 8.18.21 2 1.75mm 39s

description: UMI Matrix


title: 7.21 2 2.0mm 7s

description: UMI Matrix


title: 8.18.21 10 3.0mm 82s

description: UMI Matrix


title: 1.4.21 3 1.0mm 81s

description: UMI Matrix


title: 3.29.22 1 2.75mm 45s

description: UMI Matrix


title: 1.4.21 6 1.5mm 88s

description: UMI Matrix


title: 8.18.21 2 1.0mm 45s

description: UMI Matrix


title: 7.21 2 2.0mm 48s

description: UMI Matrix


title: 7.21 2 2.0mm 28s

description: UMI Matrix


title: 8.18.21 4 2.25mm 61s

description: UMI Matrix


title: 8.18.21 3 2.25mm 59s

description: UMI Matrix


title: 8.18.21 4 2.25mm 33s

description: UMI Matrix


title: 8.18.21 5 1.0mm 8s

description: UMI Matrix


title: 1.4.21 2 2.25mm 77s

description: UMI Matrix


title: 3.29.22 1 1.5mm 26s

description: UMI Matrix


title: 7.21 1 2.0mm 8s

description: UMI Matrix


title: 8.18.21 2 1.0mm 50s

description: UMI Matrix


title: 7.21 2 2.0mm 45s

description: UMI Matrix


title: 1.4.21 4 3.0mm 25s

description: UMI Matrix


title: 8.18.21 2 1.5mm 39s

description: UMI Matrix


title: 7.21 2 2.0mm 82s

description: UMI Matrix


title: 7.21 1 2.0mm 4s

description: UMI Matrix


title: 7.21 1 2.0mm 89s

description: UMI Matrix


title: 7.21 1 2.0mm 88s

description: UMI Matrix


title: 1.4.21 2 1.75mm 21s

description: UMI Matrix


title: 8.18.21 1 1.5mm 69s

description: UMI Matrix


title: 8.18.21 5 1.0mm 45s

description: UMI Matrix


title: 8.18.21 3 2.75mm 44s

description: UMI Matrix


title: 3.29.22 1 2.75mm 50s

description: UMI Matrix


title: 7.21 2 2.0mm 85s

description: UMI Matrix


title: 7.21 1 2.0mm 11s

description: UMI Matrix


title: 8.18.21 3 2.25mm 19s

description: UMI Matrix


title: 7.21 1 2.0mm 27s

description: UMI Matrix


title: 8.18.21 5 1.0mm 62s

description: UMI Matrix


title: 8.18.21 2 1.75mm 63s

description: UMI Matrix


title: 7.21 1 2.0mm 81s

description: UMI Matrix


title: 8.18.21 10 3.0mm 7s

description: UMI Matrix


title: 7.21 1 2.0mm 49s

description: UMI Matrix


title: 1.4.21 2 2.25mm 34s

description: UMI Matrix


title: 1.4.21 2 1.75mm 24s

description: UMI Matrix


title: 3.29.22 1 1.5mm 5s

description: UMI Matrix


title: 8.18.21 5 1.0mm 65s

description: UMI Matrix


title: 8.18.21 10 3.0mm 65s

description: UMI Matrix


title: 3.29.22 2 3.0mm 94s

description: UMI Matrix


title: 1.4.21 6 2.0mm 63s

description: UMI Matrix


title: 7.21 2 2.0mm 87s

description: UMI Matrix


title: 7.21 2 2.0mm 34s

description: UMI Matrix


title: 8.18.21 8 0.5mm 54s

description: UMI Matrix


title: 8.18.21 10 3.0mm 56s

description: UMI Matrix


title: 8.18.21 2 1.25mm 14s

description: UMI Matrix


title: 8.18.21 5 1.0mm 70s

description: UMI Matrix


title: 3.29.22 1 1.5mm 40s

description: UMI Matrix


title: 8.18.21 8 0.5mm 22s

description: UMI Matrix


title: 3.29.22 1 2.75mm 47s

description: UMI Matrix


title: 1.4.21 2 1.5mm 46s

description: UMI Matrix


title: 1.4.21 2 2.25mm 6s

description: UMI Matrix


title: 3.29.22 1 2.75mm 65s

description: UMI Matrix


title: 8.18.21 5 1.0mm 55s

description: UMI Matrix


title: 7.21 1 2.0mm 30s

description: UMI Matrix


title: 8.18.21 5 1.0mm 47s

description: UMI Matrix


title: 3.29.22 1 1.25mm 4s

description: UMI Matrix


title: 1.4.21 4 3.0mm 29s

description: UMI Matrix


title: 7.21 1 2.0mm 29s

description: UMI Matrix


title: 8.18.21 1 1.5mm 84s

description: UMI Matrix


title: 1.4.21 1.1 2.0mm 39s

description: UMI Matrix


title: 7.21 1 2.0mm 3s

description: UMI Matrix


title: 8.18.21 2 1.5mm 70s

description: UMI Matrix


title: 1.4.21 6 2.0mm 58s

description: UMI Matrix


title: 8.18.21 1 1.5mm 39s

description: UMI Matrix


title: 7.21 2 2.0mm 57s

description: UMI Matrix


title: 1.4.21 2 1.75mm 32s

description: UMI Matrix


title: 8.18.21 1 2.5mm 86s

description: UMI Matrix


title: 7.21 5 0.75mm 96s

description: UMI Matrix


title: 8.18.21 2 1.0mm 37s

description: UMI Matrix


title: 8.18.21 2 1.0mm 78s

description: UMI Matrix


title: 8.18.21 2 1.0mm 33s

description: UMI Matrix


title: 7.21 2 2.0mm 93s

description: UMI Matrix


title: 8.18.21 2 1.0mm 7s

description: UMI Matrix


title: 8.18.21 4 2.25mm 58s

description: UMI Matrix


title: 8.18.21 2 1.0mm 46s

description: UMI Matrix


title: 8.18.21 5 1.0mm 44s

description: UMI Matrix


title: 1.4.21 1 1.25mm 91s

description: UMI Matrix


title: 8.18.21 2 1.25mm 44s

description: UMI Matrix


title: 8.18.21 2 1.0mm 53s

description: UMI Matrix


title: 8.18.21 4 2.25mm 25s

description: UMI Matrix


title: 1.4.21 3 1.0mm 34s

description: UMI Matrix


title: 8.18.21 5 1.0mm 96s

description: UMI Matrix


title: 8.18.21 2 1.25mm 52s

description: UMI Matrix


title: 8.18.21 1 1.75mm 26s

description: UMI Matrix


title: 3.29.22 1 1.5mm 39s

description: UMI Matrix


title: 8.18.21 2 1.25mm 84s

description: UMI Matrix


title: 1.4.21 7 1.25mm 13s

description: UMI Matrix


title: 8.18.21 2 1.5mm 36s

description: UMI Matrix


title: 8.18.21 1 2.5mm 55s

description: UMI Matrix


title: 8.18.21 1 1.5mm 18s

description: UMI Matrix


title: 7.21 5 0.75mm 95s

description: UMI Matrix


title: 7.21 2 2.0mm 3s

description: UMI Matrix


title: 1.4.21 2 1.5mm 96s

description: UMI Matrix


title: 8.18.21 1 2.5mm 52s

description: UMI Matrix


title: 7.21 5 0.75mm 88s

description: UMI Matrix


title: 8.18.21 2 1.0mm 41s

description: UMI Matrix


title: 8.18.21 1 2.5mm 5s

description: UMI Matrix


title: 1.4.21 2 1.5mm 19s

description: UMI Matrix


title: 3.29.22 2 3.0mm 55s

description: UMI Matrix


title: 8.18.21 2 1.5mm 7s

description: UMI Matrix


title: 7.21 1 2.0mm 79s

description: UMI Matrix


title: 8.18.21 1 1.5mm 56s

description: UMI Matrix


title: 8.18.21 2 1.0mm 28s

description: UMI Matrix


title: 7.21 2 2.0mm 53s

description: UMI Matrix


title: 7.21 2 2.0mm 63s

description: UMI Matrix


title: 3.29.22 1 2.75mm 28s

description: UMI Matrix


title: 7.21 2 2.0mm 49s

description: UMI Matrix


title: 7.21 2 2.0mm 77s

description: UMI Matrix


title: 1.4.21 4 3.0mm 83s

description: UMI Matrix


title: 8.18.21 2 1.0mm 76s

description: UMI Matrix


title: 3.29.22 1 1.5mm 48s

description: UMI Matrix


title: 7.21 2 2.0mm 54s

description: UMI Matrix


title: 7.21 2 2.0mm 32s

description: UMI Matrix


title: 3.29.22 1 2.75mm 25s

description: UMI Matrix


title: 7.21 1 2.0mm 1s

description: UMI Matrix


title: 8.18.21 1 1.75mm 33s

description: UMI Matrix


title: 3.29.22 1 3.0mm 2s

description: UMI Matrix


title: 1.4.21 6 2.0mm 48s

description: UMI Matrix


title: 8.18.21 5 1.0mm 15s

description: UMI Matrix


title: 7.21 1 2.0mm 80s

description: UMI Matrix


title: 8.18.21 1 2.5mm 20s

description: UMI Matrix


title: 1.4.21 1.1 2.0mm 37s

description: UMI Matrix


title: 7.21 2 2.0mm 46s

description: UMI Matrix


title: 7.21 1 2.0mm 74s

description: UMI Matrix


title: 8.18.21 2 1.0mm 52s

description: UMI Matrix


title: 8.18.21 2 1.0mm 25s

description: UMI Matrix


title: 1.4.21 2 1.5mm 63s

description: UMI Matrix


title: 7.21 2 2.0mm 17s

description: UMI Matrix


title: 8.18.21 1 1.5mm 88s

description: UMI Matrix


title: 8.18.21 5 1.25mm 92s

description: UMI Matrix


title: 1.4.21 2 1.5mm 55s

description: UMI Matrix


title: 8.18.21 1 2.5mm 17s

description: UMI Matrix


title: 3.29.22 1 3.0mm 83s

description: UMI Matrix


title: 8.18.21 2 1.75mm 33s

description: UMI Matrix


title: 8.18.21 6 2.75mm 78s

description: UMI Matrix


title: 1.4.21 2 1.5mm 14s

description: UMI Matrix


title: 3.29.22 1 3.0mm 17s

description: UMI Matrix


title: 7.21 1 2.0mm 50s

description: UMI Matrix


title: 8.18.21 5 1.0mm 23s

description: UMI Matrix


title: 1.4.21 4 3.0mm 41s

description: UMI Matrix


title: 8.18.21 2 1.0mm 47s

description: UMI Matrix


title: 8.18.21 3 2.25mm 31s

description: UMI Matrix


title: 8.18.21 3 2.25mm 37s

description: UMI Matrix


title: 3.29.22 1 1.25mm 18s

description: UMI Matrix


title: 8.18.21 3 2.25mm 46s

description: UMI Matrix


title: 8.18.21 5 1.0mm 22s

description: UMI Matrix


title: 8.18.21 2 1.0mm 57s

description: UMI Matrix


title: 3.29.22 1 1.5mm 49s

description: UMI Matrix


title: 3.29.22 1 2.75mm 10s

description: UMI Matrix


title: 7.21 2 2.0mm 14s

description: UMI Matrix


title: 8.18.21 3 2.75mm 70s

description: UMI Matrix


title: 8.18.21 1 1.5mm 66s

description: UMI Matrix


title: 8.18.21 5 1.0mm 51s

description: UMI Matrix


title: 8.18.21 10 3.0mm 66s

description: UMI Matrix


title: 8.18.21 5 1.25mm 36s

description: UMI Matrix


title: 7.21 5 0.75mm 30s

description: UMI Matrix


title: 7.21 2 2.0mm 70s

description: UMI Matrix


title: 8.18.21 1 2.5mm 14s

description: UMI Matrix


title: 7.21 2 2.0mm 62s

description: UMI Matrix


title: 8.18.21 5 1.0mm 24s

description: UMI Matrix


title: 1.4.21 2 1.5mm 86s

description: UMI Matrix