title: PTID4_W3_Male_scATAC


title: YTL525A, GAL, rapa, MNase, 3

status: Public on Jan 11 2023

type: SRA

city: Amsterdam

first: Ineke

last: Brouwer

address: Plesmanlaan 121

country: Netherlands

institute: Netherlands Cancer Institute

library_source: genomic

data_processing:
Paired-end 2x75 bp reads were aligned to the reference genome SacCer3 (January 2015) using bowtie2 (Langmead and Salzberg, 2012) with the settings --sensitive --end-to-end -3 15 -5 5 -X 1980 --no-contain --no-discordant -p 40 -x.
aligned reads were filtered for length and only reads between 95 and 225 bp were retained for analysis
read coverage was determined on a chromosome-by-chromosome basis by counting the number of reads covering each base, and the normalized coverage was determined by normalizing the read coverage to the total coverage on the chromosome
Genome_build: sacCer3
Supplementary_files_format_and_content: paired-end sequencing reads aligned to reference sequence and filtered for length to retain reads between 95-225 bp

molecule: genomic DNA

organism: Saccharomyces cerevisiae

source_name: S. cerevisiae BY4742

growth_protocol: Cultures were inoculated and grown overnight (O/N) in Synthetic Complete (SC) medium with 2% of indicated sugar (raffinose or galactose). The following day O/N cultures were used to inoculate 250 ml cultures of SC media at OD595=0.30 and were grown until OD 0.75 at which point the culture was split into 2 seperate 100 ml cultures, of which was one was treated for 60 minutes with 7.5 uM rapamycin and the other with the same volume of DMSO.

extract_protocol:
Preparation and analysis of mono-nucleosomal DNA was performed as described previously (Donovan et al., 2019; Jonge et al., 2017) with minor modifications. Briefly, cells were grown in SC + 2% raffinose or SC + 2% galactose from OD 0.3 to OD 0.75 and then treated with 7.5 mM rapamycin or DMSO for 60 min. Then, cells were fixed in 1% PFA, washed with 1 M sorbitol, treated with spheroplasting buffer (1M sorbitol, 1 mM β-mercaptoethanol, 10 mg/ml zymolyase 100T (US biological, Z1004.250)) and washed twice with 1 M sorbitol. Spheroplasted cells were treated with 0.01171875 U (low MNase) or 0.1875 U (high MNase) micrococcal nuclease (Sigma-Aldrich, N5386-200UN) in digestion buffer (1 M sorbitol, 50 mM NaCl, 10 mM Tris pH7.4, 5 mM MgCl2, 0.075% NP-40, 1 mM β-mercaptoethanol, 0.5 mM spermidine) at 37°C. After 45 min, reactions were terminated on ice with 25 mM EDTA and 0.5% SDS. Samples were treated with proteinase K for 1 h at 37°C and decrosslinked overnight at 65°C. Digested DNA was extracted with phenol/chloroform (PCI 15:14:1), precipitated with NH4-Ac, and treated with 0.1 mg/ml RNaseA/T1. The extent of digestion was checked on a 3% agarose gel.
Sequencing libraries were prepared using the KAPA HTP Library Preparation Kit (07961901001, KAPA Biosystems) using 1 ug of input DNA, 5 uL of 10 uM adapter, double-sided size selection before and after amplification using 10 cycles. Libraries were checked on Bioanalyzer High Sensitivity DNA kit (agilent).

treatment_protocol: 60 min treatment with 7.5 uM rapamycin or same volume of DMSO

tag: strain

value: YTL525; Sth1-FRB

tag: treatment 1

value: galactose, 60 min 7.5 uM rapamycin

tag: treatment 2

value: 0.1875U Mnase

tag: replicate

value: A


title: SampleML-050

city: los angeles

first: Shubhamoy

last: Ghosh

state: CA

address: 650 Charles E Young  Dr East

department: Pediatrics

country: USA

institute: University of California Los Angeles

library_source: other

data_processing:
Demultiplexed sequence data were imported to QIIME2 and quality filtering was performed using DADA2to ensure adequate sequence quality
A feature table along with a representative sequence table containing amplicon sequence variants (ASV) were generated and a rooted phylogenetic tree was constructed based on sequence similarity to infer the evolutionary relationship between microorganisms.
The outputs from QIIME2 (feature table, taxonomic classifications, phylogenetic tree) along with the corresponding metadata, were imported to R (version 4.2.1) and assembled into a phyloseq object using the phyloseq package (version 1.40.0)
all data visualization steps were performed using ggplot2 package in R

molecule: other

organism: Rattus norvegicus

source_name: Sprague Dawley

growth_protocol: Pregnant Sprague-Dawley rats (Charles River Laboratories, Hollister, CA) were housed in individual cages at 21-23C within 12h light/dark cycles.

extract_protocol:
fecal samples from within the intestines of Day2, Day21 and adult offspring (Day180) were collected and bacterial genomic DNA was extracted for 16S rDNA microbiota profiling
The V4 region of the 16S rRNA gene was amplified with individually barcoded universal primers. PCR amplified products were purified and pooled in equimolar concentrations and sequenced using the Illumina MiSeq platform and 2 X 250bp reagent kit for paired-end sequencing.

treatment_protocol: At gestational day 11  dams were randomized to either continue regular chow (CON group) or on a Western diet ad libitum (HFhf group)along with 25% fructose in their drinking water.The pregnant dams in the IUGR group were 50% calorie restricted beginning from gestation day (G) 10 in their access to regular chow diet until the birth of their pups.after birth, the maternal diet was switched to either regular chow accessed ad libitum forming the IUGR-RC (regular chow) group, or a HFhf diet creating the   IUGR-HFhf group

tag: Sex

value: F

tag: age (day)

tag: collection time

value: PM

value: Sprague Dawley

tag: specimen

value: feces

tag: group

value: IUGR-HFhf

tag: molecule

value: 16S rDNA


title: PTID2_W7_Male_scATAC

city: Seattle

first: Allen Institute

last: For Immunology

state: Washington

address: 615 Westlake Ave N, Seattle, WA

institute: Allen Institute

description: The FASTQs are from human subjects going into dbGAP so they are not listed here

data_processing:
Binary Base Call (BCL) files were demultiplexed into FASTQ files was performed using 10x cellranger mkfastq (10x Genomics v.3.1.0)/cellranger-atac mkfastq (10x Genomics v.1.2.0).
10x cellranger/cellranger-atac count was used to process sequencing reads by performing adapter trimming and sequence alignment to the GRCh38 (hg38) reference genome (refdata-cellranger-atacGRCh38-1.1.0). The output files for rna: atac: fragments.tsv.gz and singlecell.csv were utilized for downstream processing and quality control analysis.
Hash Tag Oligo Counting: BarCounter v1.0 ; Default Parameters
HDF5 files (filtered) were then uploaded into R statistical programming language using the Seurat package (version 4.0) where normalization, scaling, integration and reference based label transfer was performed.
scATAC based fragments were submitted to ArchR package to create ArchR object for qulity analysis and downstram analysis
Flow Raw data were compensated and curated to remove unrepresentative events due to instrument fluidics variability (time gating), doublets (by FSC-H and FSC-W), and cells exhibiting membrane permeability (live/dead gating) prior to quantification using BD FlowJo software.
For Olink data, quality assessment, limited detection, and normalization were performed by using the plate bridging control, two positive controls, and three background controls.
Genome_build: Ensembl GRCh38-3.0.0
Supplementary_files_format_and_content: Fragment TSVs (Tab separated), Single cell CSVs (Commas separated),  HDF5 file (Hierarchical Data Format), RDS (R Object Data), Rda (R Data).

organism: Homo sapiens

source_name: peripheral blood mononuclear cells

extract_protocol:
30 mL of blood was drawn into BD NaHeparin vacutainer tubes (for PBMC; BD #367874) or K2-EDTA vacutainer tubes (for plasma; BD #367863). Upon arrival at the processing lab all NaHeparin tubes for each donor were pooled into a sterile plastic receptacle to establish one common pool for all time points and stored at room temperature until processing (4 hours or less from draw). PBMC were isolated by Ficoll density gradient separation and cryopreserved by a team of operators, as previously described (https://doi.org/10.1016/j.isci.2021.102404). Thawed PBMC were assayed by cytometry and single-cell RNA sequencing in two batches (donors 5206BW and 7626BW, donors 1178BW and 2216BW) by a team of operators. Plasma was isolated and cryopreserved by a team of operators, as previously described (https://doi.org/10.1016/j.isci.2021.102404).
Single-cell RNA-seq libraries were generated using the 10x Genomics Chromium 3’ Single Cell Gene Expression assay (#1000121) and Chromium Controller Instrument according to the manufacturer’s published protocol with modifications for cell hashing21. To block off-target antibody binding, Blocking Solution (5 µL of Human Trustain FcX (BioLegend #422302), and 13.7 µL of a 10% Bovine Serum Albumin (BSA)) was added to 500,000 cells suspended in 50 µL Dulbecco’s Phosphate Buffered Saline (DPBS; Corning Life Sciences #21-031-CM) and incubated for 10 minutes on ice. To stain samples, 0.5 µg (1 µL) of a TotalSeq™-A anti-human Hashtag Antibody was suspended in 31.3 µL DPBS/2% BSA, then added to each sample. For each batch of samples, 100,000 cells from 12 hashed samples with a distinct Hashtag Antibody were pooled into the hashed poolPool 1; 8 additional samples were pooled into hashed pool. Roughly 20,000 cells from a Leukopak healthy control were also labeled with a distinct TotalSeq™-A Hashtag Antibody and were spiked into each pool to serve as a batch control. scATAC-seq libraries were prepared according to the Chromium Single Cell ATAC v1.1 Reagent Kits User Guide (CG000209 Rev B) with several modifications. 195,000 cells were loaded into each tagmentation reaction. Permeabilized cells were brought up to a volume of 129 µl in TD1 buffer (Illumina, 15027866) and mixed with 36 µl of Illumina TDE1 Tn5 transposase (Illumina, 15027916). Transposition was performed by incubating the prepared reactions on a C1000 Touch thermal cycler with 96–Deep Well Reaction Module (Bio-Rad, 1851197) at 37°C for 60 minutes, followed by a brief hold at 4°C. A Chromium NextGEM Chip H (10x Genomics, 2000180) was placed in a Chromium Next GEM Secondary Holder (10x Genomics, 3000332) and 50% Glycerol (Teknova, G1798) was dispensed into all unused wells. A master mix composed of Barcoding Reagent B (10x Genomics, 2000194), Reducing Agent B (10x Genomics, 2000087), and Barcoding Enzyme (10x Genomics, 2000125) was then added to each sample well, pipette-mixed, and loaded into row 1 of the chip. Chromium Single Cell ATAC Gel Beads v1.1 (10x Genomics, 2000210) were vortexed for 30 seconds and loaded into row 2 of the chip, along with Partitioning Oil (10x Genomics, 2000190) in row 3. A 10x Gasket (10x Genomics, 370017) was placed over the chip and attached to the Secondary Holder. The chip was loaded into a Chromium Single Cell Controller instrument (10x Genomics, 120270) for GEM generation. At the completion of the run, GEMs were collected, and linear amplification was performed on a C1000 Touch thermal cycler with 96–Deep Well Reaction Module: 72°C for 5 min, 98°C for 30 sec, 12 cycles of: 98°C for 10 sec, 59°C for 30 sec and 72°C for 1 min. Flow cytometry was performed as previously described (https://doi.org/10.1016/j.isci.2021.102404). In brief, cryopreserved PBMC were thawed, washed, and counted. 1-2 x106 cells were incubated with Human TruStain FcX (BioLegend #422302) and Fixable Viability Stain 510 (BD #564406) prior to staining with a 25-color cell surface panel (Key Resources Table) on ice for 25 minutes. Cells were washed and fixed with 4% paraformaldehyde (Electron Microscopy Sciences #15713) prior to acquisition on a BD Symphony cytometer. Plasma samples were submitted to Olink (Uppsala, Sweden) for assay using the Olink Proximity Extension assay, run on the Fluidigm Biomark system. Patient samples were distributed evenly across two plates, and all time points per patient were run on the same plate, with randomized well locations. Samples were assayed using the Olink Discovery Assay which encompasses all proteins across 13 panels (Cardiometabolic [V.3603], Cardiovascular II [V.5006], Cardiovascular III [V.6113], Cell Regulation [V.3701], Development [V.3512], Immune Response [V.3202], Inflammation [V.3021], Metabolism [V.3402], Neuro Exploratory [V.3901], Neurology [V.8012], Oncology II [V.7004], Oncology III [V.4001], Organ Damage [V.3311]).
Other: scRNA-seq, scATAC-seq, FlowCytometry, Olink, CBC

value: Male


title: YTL525A, GAL, rapa, MNase, 1


title: PTID2_W6_Male_scATAC


title: P4492

city: Edmonton

first: Graham

last: Plastow

state: Alberta

address: 1400 College Plaza, 8215 - 112 Street

department: Agricultural, Food & Nutritional Science

country: Canada

institute: University of Alberta

library_source: transcriptomic

data_processing:
The raw reads were concatenated as there were two files for each read (run on seperate lanes), then trimmed so those samples with quality scores < 20 were filtered, and  mapped to bovine genome (ARS-UCD1.2.98;https://bovinegenome.elsiklab.missouri.edu/downloads/ARS-UCD1.2 ) using the STAR software. The number of reads uniquely aligned to each gene was calculated using FeatureCounts.
Assembly: ARS-UCD1.2
Supplementary files format and content: Matrix table with raw gene counts for every gene and every sample

molecule: total RNA

organism: Bos taurus

source_name: Blood

extract_protocol:
Total RNA was extracted from Tempus tubes using a Preserved Blood RNA Purification Kit (Norgen Biotek Corp.,Thorlord ON, ), and the Nanodrop 1000 (Thermo Scientifc, Willmingotn, DE, USA) and 2200 RNA Screentape Tapestation System (Agilent Technologies Inc, Cedar Creek, TX) were used respectively to quantify and measure the RNA integrity Number for each sample.
All samples had RIN values above 8,0 and were all used for library construction and RNA-seq was performed on the Novaseq platform running 50 samples per lane on 2 lanes.
cDNA libraries were prepared for sequencing for each individual animal from the high-quality RNA using the TruSeq RNA Library Preparation kit v2 (Illumina, San Diego, CA, United States) and the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs Ltd., Whitby, ON, Canada).

tag: disease status

value: Control (pen-matched)


title: Y3660


title: P4475


title: G3742


title: P4467


title: SampleML-049


title: G3594


title: PTID2_W5_Male_scATAC


title: SampleML-048

value: IUGR-RC


title: G3474


title: YTL524A, GAL, rapa, MNase, 1

value: YTL524; Swi2-FRB


title: SampleML-047


title: G3473


title: SampleML-046


title: YTL524A, GAL, -rapa, MNase, 3

value: galactose, 60 min DMSO


title: G3723


title: PTID2_W3_Male_scATAC


title: PTID2_W2_Male_scATAC


title: SampleML-045


title: IMM19_711_scRNA


title: YTL524A, GAL, -rapa, MNase, 1


title: YTL525A, GAL, -rapa, MNase, 3


title: Y3243


title: G3315


title: perfused

city: Hamburg

first: YU

last: ZHAO

address: Martinistr. 52

country: Germany

institute: Hamburg Center for Translational Immunology

description: 10x Genomics

library_source: transcriptomic single cell

data_processing:
Preprocessing and QC. 10x Genomics raw sequencing data were processed using CellRanger software (version 5.0.1, 10x Genomics, Pleasanton, CA).
Pig genome was built by function “cellranger mkref” using the Sscrofa v103 FASTA file and GTF file (https://www.ensembl.org) and then mapping was done and the count matrices were generated by function “cellranger count” with parameter “include-introns".
Assembly: Sus_scrofa
Supplementary files format and content: 10x standard output including matrix table with raw gene counts for every gene and every sample

organism: Sus scrofa

source_name: kidney

extract_protocol: Chromium NEXT GEM Single Cell 3’ Reagent kits v3.1 according to manufacturer protocol

tag: tissue

value: kidney


title: Y2992


title: IMM19_710_scRNA


title: PTID6_W7_Male_scRNA


title: YTL525A, GAL, -rapa, MNase, 1


title: unperfused

description: 10x Genomics


title: PTID6_W6_Male_scRNA


title: WM276, Pro, NT, biol rep 3

city: Bethesda

first: Yun

last: Chang

address: 9000 Rockville pike

institute: NIH

data_processing:
The RNA-seq data were analyzed by Partek® Flow® software, version 7.0, 2017 (Partek Inc., St. Louis, MO).
Illumina reads were aligned to the reference genome of WM276 (ASM18594v1) using STAR.
After trimming and mapping, approximately 15–38 million total paired aligned reads were recovered from each of the RNA-Seq libraries.
DESeq2 was used to calculate the differentially expressed genes. Differential expression deemed significant if the changes are at least 2-fold and p-adj is < 0.05.
Assembly: reference genome of WM276 (ASM18594v1)
Supplementary files format and content: Transcript counts in excel file (xlsx form)
Supplementary files format and content: Normalized data by DESeq2 in excel file (xlsx form)

organism: Cryptococcus gattii

growth_protocol: Cells were grown in 20 ml YPD medium at 30℃ for 16 h. Then, about 5 ml of the overnight culture was inoculated into 100 ml of fresh YPD medium to make the OD600 of culture as 0.4 and further incubated for 4-5 h at 30℃ until OD600 of culture medium reached approximately 1.0. The cultures were divided in four of 50 ml tubes with 20 ml each.

extract_protocol:
Total RNAs were isolated by the Trizol reagent (Invitrogen, Carlsbad, CA) and RNAeasy mini kit (Qiagen). RNAs were treated with Turbo RNase-free DNase (Ambion) to remove genomic DNA.
polyA-RNA was purified from total RNA, processed, and paired-end directional libraries (75 bp) were prepared and sequenced using an Illumina HiSeq 4000 platform (Novogene, Davis, CA).

treatment_protocol: The cells were pelleted by centrifugation at 3500 rpm for 5 min and washed twice with 10 ml of YNB without ammonium sulfate without amino acid medium. The cells were resuspended in 10 ml of YNB with NH4, Asn, or Pro media with or without 20 µg/ml 5-FC. The cells were further incubated for 2 hours at 30°C and pelleted by centrifugation at 3500 rpm for 5 min and then supernatant medium was discarded. Then, the cells were frozen in dry ice with ethanol and lyophilized overnight.

tag: cell line

tag: genotype

value: WT

tag: treatment

value: Pro


title: R21


title: WM276, Pro, 5-FC, biol rep 3

value: Pro, 5-FC


title: SampleML-039

value: AM


title: SampleML-037


title: Y3460


title: SampleML-036


title: PTID2_W4_Male_scATAC


title: WM276, Asn, 5-FC, biol rep 3

value: Asn, 5-FC


title: R1091


title: PTID6_W4_Male_scRNA


title: WM276, Asn, NT, biol rep 3

value: Asn


title: SampleML-041

value: M


title: SampleML-043

value: Sprague Dawley


title: R1122


title: SampleML-035

value: F

value: feces


title: WM276, NH4, 5-FC, biol rep 3

value: NH4, 5-FC


title: PTID6_W3_Male_scRNA

value: Male


title: R107

value: Control (pen-matched)


title: SampleML-034

value: IUGR-HFhf

value: 16S rDNA


title: PTID6_W2_Male_scRNA


title: R1027


title: WM276, NH4, NT, biol rep 3


title: R1011


title: SampleML-033


title: PTID5_W7_Female_scRNA

value: Female


title: SampleML-032


title: WM276, Pro, 5-FC, biol rep 2


title: PTID5_W6_Female_scRNA


title: Y9682

value: BRD


title: WM276, Pro, NT, biol rep 2

value: WT


title: PTID6_W5_Male_scRNA


title: PTID5_W5_Female_scRNA


title: WM276, Asn, 5-FC, biol rep 2


title: R9489


title: SampleML-029

value: IUGR-RC


title: SampleML-030


title: PTID5_W4_Female_scRNA


title: PTID5_W3_Female_scRNA


title: SampleML-031


title: R8966


title: SampleML-028


title: WM276, NH4, 5-FC, biol rep 2


title: WM276, NH4, NT, biol rep 2


title: U6974


title: WM276, Asn, NT, biol rep 2


title: SampleML-027


title: R8788


title: WM276, Pro, 5-FC, biol rep 1

value: Pro, 5-FC


title: SampleML-026

value: AM


title: PTID4_W7_Male_scRNA


title: U6921


title: WM276, Pro, NT, biol rep 1

value: Pro


title: PTID4_W6_Male_scRNA


title: U6892


title: SampleML-025


title: PTID4_W5_Male_scRNA


title: WM276, Asn, 5-FC, biol rep 1

value: Asn, 5-FC


title: PTID5_W2_Female_scRNA


title: U6879


title: PTID4_W4_Male_scRNA


title: U6792


title: Y9745


title: U6679


title: WM276, Asn, NT, biol rep 1

value: Asn


title: SampleML-023

value: Sprague Dawley


title: SampleML-022

value: M


title: PTID4_W2_Male_scRNA


title: PTID4_W3_Male_scRNA


title: SampleML-021

value: feces


title: SampleML-020

value: IUGR


title: U5669


title: SampleML-019


title: O5245


title: PTID2_W6_Male_scRNA

value: Male


title: O5108


title: PTID2_W7_Male_scRNA


title: Vegetative growing cells, 240 min after elutriation, A

city: Bryan

first: GEOFFREY

last: KAPLER

state: TX

address: 4209B Medical Research Education Building II

institute: Texas A&M University

description: A

data_processing:
After sequencing, adapters and low quality reads were removed by Trimmomatic, paired reads with concordant mappings were aligned to the Tetrahymena genome  by HISAT2, the abundance of these transcripts in each sample was computed using StringTie.
Assembly: Tetrahymena genome (SB210, 2021 version, TGD)
Supplementary files format and content: tab-delimited text file includes count values for each Sample

organism: Tetrahymena thermophila

growth_protocol: CU428 cells (1.5 L) were grown to ~1x10^5 cells/ml in 2% PPYS/1000 U/ml pen-strep (Sigma Aldrich)  at 30˙C.

extract_protocol:
For RNA isolation, cell pellets were resuspended in 1 ml RNAlater and placed at 4˙C overnight. RNAlater was removed and RNA prepared using the Qiagen RNeasy kit with additional DNAse digestion on the column.
10 ug of total RNA were used to make cDNA library was prepared using Illumina RNA-seq Prep Kit (Illumina).
Illumina-HiSeq 2500v4

treatment_protocol: Elutriation was used to obtain a synchronized population of cells during vegetative growth. Cells were loaded into the elutriation centrifuge at 850 rpm, 50 ml/min. G1 cells were collected at 60 ml/min (200 ml) and placed in a 2L flask at 30˙C, shaking at 100 rpm. Cells were collected at 30 min time intervals up to 240 min. For each time point, 20 ml of cells were collected, 5 ml prepared for FACs analysis, and 15 ml for isolation of RNA. Cells were centrifuged and cell pellets were washed in 10 mM Tris pH 7.4 before preparation for FACs and RNA isolation.

tag: cell type

value: vegetative growing cells

value: elutriation synchronized

tag: time

value: 240 min


title: SampleML-024

value: F

tag: strain

value: 16S rDNA


title: Vegetative growing cells, 210 min after elutriation, B

description: B

value: WT

value: 210 min


title: PTID2_W3_Male_scRNA


title: SampleML-016


title: SampleML-018


title: PTID2_W2_Male_scRNA


title: O5076

value: BRD


title: WM276, NH4, NT, biol rep 1


title: Vegetative growing cells, 180 min after elutriation, B

description: B

value: 180 min


title: Vegetative growing cells, 240 min after elutriation, B

description: B


title: O5096


title: Vegetative growing cells, 150 min after elutriation, B

description: B

value: 150 min


title: PTID2_W5_Male_scRNA


title: R493


title: IBC Tumor Panitumumab-treated, Sample 1

city: Houston

first: Ganiraju

last: Manyam

address: 1515 Holcombe Blvd

institute: UT MD Anderson Cancer Center

description: IBC tumor cells from Humanized mouse

data_processing:
Sample preparation and sequencing were performed by the MD Anderson Cancer Center Sequencing and Microarray Facility
The raw reads were mapped to the human reference genome (GRCh38) using the cellranger count pipeline
The digital expression matrix was extracted from the filtered_feature_bc_matrix folder outputted by the cellranger count pipeline.
Genome_build: GRCh38
Supplementary_files_format_and_content: Transcript-Level Read Count files

source_name: IBC Tumor

extract_protocol:
When drug treatment was completed, mice were euthanized, and tumor samples were subjected to single-cell dissociation for FACS analysis and scRNA-seq
Samples were normalized for input onto the Chromium Single Cell Chip B Kit (10x Genomics, Pleasanton, CA), in which single cells were lysed and barcoded for reverse-transcription. The pooled single-stranded, barcoded cDNA was amplified and fragmented.
Single Cell RNAseq

tag: disease state

value: Inflammatory Breast Cancer

value: Panitumumab

value: IBC Tumor


title: O4919


title: SampleML-014

value: M

value: AM

value: Sprague Dawley


title: SampleML-013

value: feces

value: IUGR


title: IBC Tumor Panitumumab-treated, Sample 2

description: IBC tumor cells from Humanized mouse


title: Vegetative growing cells, 180 min after elutriation, A

description: A

value: vegetative growing cells

value: elutriation synchronized


title: Vegetative growing cells, 120 min after elutriation, B

description: B

value: 120 min


title: IBC Tumor, IgG2 Control, Sample 2

description: IBC tumor cells from Humanized mouse

value: Control


title: R50


title: PTID2_W4_Male_scRNA

value: Male


title: Vegetative growing cells, 210 min after elutriation, A

description: A

value: WT

value: 210 min


title: SampleML-017

value: 16S rDNA


title: Vegetative growing cells, 150 min after elutriation, A

description: A

value: 150 min


title: U4915

value: BRD


title: IBC Tumor, IgG2 Control, Sample 1

description: IBC tumor cells from Humanized mouse

value: Inflammatory Breast Cancer


title: SampleML-012


title: Vegetative growing cells, 120 min after elutriation, A

description: A


title: SampleML-011


title: SampleML-015


title: Vegetative growing cells, 90 min after elutriation, B

description: B

value: 90 min


title: P4744


title: SampleML-010

value: F


title: IMR90-CM SD

city: HK ISLAND

first: KM

last: NG

address: L503, LAB BLOCK,FMB, 21 Sassoon rd

department: Medicine

country: Hong Kong

institute: The University of Hong Kong

data_processing:
Basecalls performed using CASAVA 1.8.2
Sequencing reads were first filtered for adapter sequence and reads with read length ≥ 40bp were mapper to Human Genome GRCh38 (downloaded from GENCODE) using STAR Version 2.5.2.
RSEM Version 1.2.31 was used for  quantifying expression and EBSeq Version 1.18.0 was used to identify differentially expressed genes
Genome_build: Human Genome GRCh38
Supplementary_files_format_and_content: Microsoft excel  file lsiting the relative gene expressions of the samples.

source_name: iPSCs-derived cardiomyocytes

growth_protocol: iPSCs-derived cardiomyocytes were maintened in RPMI/B27

extract_protocol:
Total RNA was isolated using the mirVana miRNA Isolation Kit (Ambion).Ribosomal RNA (rRNA) depletion was performed using NEBNext® rRNA Depletion Kit (Human/Mouse/Rat).
cDNA libraries were prepared by NEBNext® Ultra II Directional RNA Library Prep Kit

value: cardiomyocytes

tag: genotype/variation

value: normal female iPSCs (line IMR90 WiCell)


title: O5003


title: P4735


title: DL1-CM SD

value: wildtype MeCP2, isogenic to 72B


title: O4713


title: 72B-CM SD

value: expressing MeCP2-1155del32


title: Vegetative growing cells, 90 min after elutriation, A

description: A

value: vegetative growing cells


title: Vegetative growing cells, 60 min after elutriation, A

description: A

value: elutriation synchronized

value: 60 min


title: Vegetative growing cells, 60 min after elutriation, B

description: B


title: P4669


title: SampleML-008

value: AM

value: Sprague Dawley

value: feces

value: IUGR


title: P4693


title: SampleML-009

value: 16S rDNA


title: TERT promoter mutant secondary sphere [C228TWT2_S2]

city: La Jolla

first: Frank

last: Furnari

state: California

address: 9500 Gilman Dr, CMME2021

institute: UCSD

data_processing:
aligned with STAR aligner
reads were counted using featurecounts
Genome_build: hg19
Supplementary_files_format_and_content: RNAseq_counts.csv

source_name: The secondary tumor sphere derived from the primary sphere

growth_protocol: DMEM/F12 with GlutaMAX (Thermo Fisher Scientific) with 1 x B-27 supplement, 20 ng/mL EGF and 20 ng/mL bFGF (Stemcell Technologies)

extract_protocol:
RNeasy Plus Kits - QIAGEN
polyA enrichment library preparation (Novogene)

tag: tissue source

value: human iPSC derived NPC derived tumor

tag: genotype/tissue type

value: TERT promoter mutant secondary sphere


title: TERT promoter mutant secondary sphere [C228TWT1_S1]


title: Vegetative growing cells, 30 min after elutriation, B

description: B

value: WT

value: 30 min


title: SampleML-006

tag: Sex

value: F

tag: age (day)


title: TERT promoter mutant secondary sphere [C228TWT2_S1]


title: P4666

value: BRD


title: Vegetative growing cells, 30 min after elutriation, A

description: A


title: SampleML-005

tag: collection time

tag: specimen


title: O4638


title: TERT promoter WT type secondary sphere [WTWT_S2]

value: TERT promoter WT type secondary sphere


title: O4492


title: TERT promoter WT type secondary sphere [WTWT_S1]


title: SampleML-003

tag: group

tag: molecule


title: TERT promoter WT type secondary sphere [Parental_S2]


title: Vegetative growing cells, 0 min after elutriation, A

description: A

value: 0 min


title: Vegetative growing cells, 0 min after elutriation, B

description: B


title: SampleML-004


title: O4474

tag: disease status


title: SampleML-007


title: TERT promoter mutant primary sphere [C228TWT2_P2]

source_name: NPC derived tumor sphere

value: TERT promoter mutant primary sphere


title: SampleML-001


title: Retina tissue, WT animal, room air exposed, GSD379

city: Miami

first: Augusto

middle: F

last: Schmidt

state: FL

address: 1611 NW 12th ave

institute: University of Miami Miller School of Medicine

description:
GSD379
column 11 retina_counts.txt
retina_counts.txt

data_processing:
Sequence reads were checked for quality with fastqc
Reads were aligend using Kallisto
Assembly: mm10
Supplementary files format and content: tab-delimited text file of pseudocounts for each sample

organism: Mus musculus

source_name: retina

extract_protocol:
RNA was extracted Rneasy mini plus kit (Qiagen). 1ug  of total RNA was used for the construction of sequencing libraries.
RNA libraries for RNA-seq were prepared at BGI Genomics

treatment_protocol:
Adipogenic differentiation was induced by treatment of 2-day post confluent cells (designated day 0, D0) with 10ug/ml insulin (Sigma),
1uM dexamethasone (Sigma), and 0.5mM isobutylmethylxanthine (Sigma) in the presence of 10% FBS until day 2. Cells were then fed with DMEM supplemented with 10% FBS and 10ug/ml insulin for 2 days, after which they were fed every other day with DMEM containing 10% FBS

tag: tissue

value: retina

value: Room air


title: SampleML-002


title: Retina tissue, WT animal, room air exposed, GSD383

description:
GSD383
column 13 retina_counts.txt
retina_counts.txt

tag: genotype


title: O4328


title: TERT promoter mutant primary sphere [C228TWT2_P1]


title: G3754


title: Retina tissue, WT animal, hyperoxia exposed, GSD350

description:
GSD350
column 10 retina_counts.txt
retina_counts.txt

tag: treatment

value: Hyperoxia


title: MM-seq_lambda_mES_KO

city: Guangzhou

first: Zhang

last: Zhang

state: Guangdong

address: Xingangxi Roda 135#

country: China

institute: Sun Yat-sen University

data_processing:
library strategy: MM-seq (Modification-induced Mismatch Sequencing)
Raw reads were firsly removed sequencing adaptors by cutadapt (v. 1.15). Clean reads were mapped to reference genome using bowtie2 (v 2.3.3) with default parameters except one mismatch. The sorted bam file was generated using samtools (v1.6; ref). Integrative Genomics Viewer (IGV) was used for data visualization. Picard (v1.129) MarkDuplicates was used to mark and remove duplicates.
For MM-seq data using T7E1/MBN endonuclease, we discarded single-end mapping results and counted both 5’ and 3’ terminals of each fragment as potential T7E1 digest sites. For MM-seq data using lambda exonuclease, only 5’ terminal of sequenced fragments were calculated. A binominal distribution model was assumed that each fragment could be randomly sheared or cleavage by structure-sensitive enzymes. The P-values were further corrected by Bonferroni correction, and FDR≤0.01 was set as the cutoff.
Genome_build: human (hg19), mouse (mm10), Chlamydomonas reinhardtii (v5.5), Escherichia coli (ensembl release-45)
Supplementary_files_format_and_content: genomic positions of 6mA

source_name: mouse embryonic stem cell line

growth_protocol: Chlamydomonas reinhardtii was cultured in Tris Acetate Phosphate (TAP) medium with modified trace element solution (pH 7.5) at 110 rpm at 22 ℃. Human cell lines were cultured in 10% (v/v)  FBS (Gibco) containing DMEM (Corning) at 37 °C under 5 % CO2 and passed mycoplasma contamination test.

extract_protocol:
Chlamydomonas reinhardtii: cells were treated with lysis buffer (50mM Tris-HCl(pH8.0), 200mM NaCl, 20mM EDTA, 2% SDS, 1mg/ml Proteinase K) and CTAB buffer (50mM Tris-HCl (pH8.0), 20mM EDTA, 1.4M NaCl, 2% CTAB, 1% PVP4000) at 65℃ for 30 min. Total RNA was digested using 100 µg/ml RNase A for 20 min at room temperature. Genomic DNA was isolated by phenol-chloroform extraction and ethanol precipitation. E. coli: Genomic DNA was extracted using FastPure Bacteria DNA Isolation Mini Kit (Vazyme, DC103). Cell lines: Cells were collected and then lysed by lysis buffer (0.1% TritonX-100, 0.1% Tween-20 diluted in 1× PBS) supplemented with 5 μg Proteinase K (Sangon) and 2 μl RNase A (Takara) under the condition of shaking (~1000 rpm) at 37 ℃ for 30 min. E. coli: MinElute PCR Purification Kit (QIAGEN, 28006) was used to isolate genomic DNA.
During MM-seq with endonuclease digestion, 6mA immunoprecipitation reaction was conducted in total volume of 500μL with 4μg fragmented gDNA, 5μL (0.5mg/mL) anti-m6A monoclonal antibody (CST), 100μL 5×IP Buffer, 30μL pre-block protein-A magnetic beads. The mixture was transferred to head-over-tail rotating wheel to incubate at 4 °C overnight and placed on a magnetic rack until beads are captured. The supernatant was discarded and the beads was washed with 500 μL ice-cold 1×IP Buffer by gentle vortex. The beads were re-suspended in the T7E1 or MBN digestion system (M0302S or M0250S, NEB) and incubated for 30 min at 37 °C. After washed with 500 μL ice-cold 1×IP Buffer, 5μL Proteinase K was added to the beads and incubated for 2h at 65 °C with 1,000 rpm. The products were purified by Zymo Research oligo Clean & Concentrator and eluted in 12 μL ddH2O. NGS libraries were constructed for products from genomic DNA using NEBNext Ultra II DNA Library Prep Kit (E7645S). Same procedure about 6mA immunoprecipitation reaction was applied when using exonuclease cleavage in MM-seq. The beads with IP products were conducted primer extension, end-repair, adaptor ligation and primer extension steps using T4 DNA polymerase (M0203S, NEB), T4PNK (T4 Polynucleotide Kinase, M0201S, NEB), T4 DNA ligase (M0202S, NEB) and Phi29 DNA polymerase (M0264S, NEB), respectively. The beads were washed by 500 μL ice-cold 1×IP Buffer after each treatment. The beads were resuspended in the lambda exonuclease (M0262S, NEB) digestion system incubating for 30 min at 37 °C. After washed by 1×IP Buffer, the beads were treated by 5μL Proteinase K to digest antibody. The mixture were incubated for 2h at 65 °C with 1,000 rpm speed. Then the beads were spin down briefly and placed the tube on magnetic rack for 30s until beads were captured and transferred the supernatant in a new tube. The products were purified by Zymo Research oligo Clean & Concentrator and elute in 12 μL ddH2O. After primer extension by Phi29 DNA polymerase, the products were added into R2 Adapter ligation reaction. Then, the products were clean up by using 52 μL well-mixed AMPure beads and eluted dsDNA fragment in 30 μL ddH2O. After the PCR amplification, the library was purified using 50 μL well mixed AMPure beads or agarose gel size selection.

tag: cell line or tissue

value: mouse 129 embryonic stem cells

tag: sequence type

value: MM-seq by lambda exonucleases

value: Mettl3 knock-out


title: Y3735


title: TERT promoter mutant primary sphere [C228TWT1_P2]


title: Retina tissue, WT animal, hyperoxia exposed, GSD347

description:
GSD347
column 9 retina_counts.txt
retina_counts.txt


title: MM-seq_lambda_mES_WT

value: wide type


title: TERT promoter WT type primary sphere [WTWT_P2]

value: TERT promoter WT type primary sphere


title: TERT promoter mutant primary sphere [C228TWT1_P1]

value: human iPSC derived NPC derived tumor


title: Retina tissue, KO animal, hyperoxia exposed, GSD310

description:
GSD310
column 7 retina_counts.txt
retina_counts.txt

value: GSDMD KO


title: Chicken spleens from infection group (b8)

city: Beijing

first: Jiahui

last: Shi

address: 38 Life Science Park Road, Changping District, Beijing

institute: National Center for Protein Sciences (Beijing)

data_processing:
cDNA libraries were sequenced by paired-end 150 bp sequencing on an Illumina system.
Clean data were obtained by removing reads containing adapter, reads containing poly-N and low quality reads from raw data. At the same time, Q20, Q30 and GC content the clean data were calculated
Transcript count, FPKM were calculated using a protocol.
Genome_build: Genome of G. gallus.
Supplementary_files_format_and_content: Table with raw gene counts for every gene and every sample

organism: Gallus gallus

source_name: Spleen

growth_protocol: The one-day-old white leghorn SPF chickens were reared in isolators for 7 days, then these chickens were transferred to fumigated animal houses and reared for another 7 days. All the chickens were proved to be adapted to the breeding environment by 15 days old. After weighing, the chickens were randomly divided into two groups and 48 chickens in each group.

extract_protocol:
Briefly, total RNA was extracted with Trizol method. The NEBNext® Ultra™ Directional RNA Library Prep Kit (Illumina, San Diego, CA, USA) was used to prepare the cDNA libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols

treatment_protocol: The chickens in infection group were injected in the chest muscles with 0.5 mL of E. coli O78 bacterial fluid, while the chickens in control group were injected in the chest muscles with 0.5 mL sterile normal saline.

value: Spleen

tag: breed

value: white leghorn

tag: condition

value: SPF

tag: infection

value: E. coli O78

tag: repetition

value: Biological repetition


title: Chicken spleens from infection group (b9)


title: O3720

value: BRD


title: Chicken spleens from infection group (b7)


title: G3630


title: Retina tissue, KO animal, hyperoxia exposed, GSD308

description:
GSD308
column 6 retina_counts.txt
retina_counts.txt


title: O4349


title: TERT promoter WT type primary sphere [WTWT_P1]

tag: tissue source

tag: genotype/tissue type


title: WM276, NH4, 5-FC, biol rep 1

tag: cell line

value: WT

value: NH4, 5-FC


title: Retina tissue, WT animal, hyperoxia exposed, GSD345

description:
GSD345
column 8 retina_counts.txt
retina_counts.txt

value: retina


title: G3614


title: TERT promoter WT type primary sphere [Parental_P2]


title: G3635


title: Retina tissue, WT animal, room air exposed, GSD382

description:
GSD382
column 12 retina_counts.txt
retina_counts.txt

value: Room air


title: Retina tissue, KO animal, hyperoxia exposed, GSD306

description:
GSD306
column 5 retina_counts.txt
retina_counts.txt

value: Hyperoxia


title: Chicken spleens from infection group (b5)


title: Retina tissue, KO animal, room air exposed, GSD304

description:
GSD304
column 4 retina_counts.txt
retina_counts.txt


title: Chicken spleens from infection group (b6)


title: Chicken spleens from infection group (b4)


title: R35


title: Day16_AM_diff

first: Hannele

last: Ruohola-Baker

state: WA

address: UW Medicine at South Lake Union, 850 Republican Street

department: Department of Biochemistry

institute: University of Washington

data_processing:
All libraries were sequenced on one NovaSeq platform (Illumina). The recipe used was: read 1: 34 cycles, read 2: 100 cycles, index 1: 10 cycles, index 2: 10 cycles. Base calls, downstream sequence processing and single-cell digital-expression matrix generation steps were similar to what was described in sci-RNA-seq3 paper17. STAR v.2.5.2b54 aligner used with default settings and gene annotations (GRCh38-primary-assembly, gencode.v27). Uniquely mapping reads were extracted, and duplicates were removed using the UMI sequence, reverse transcription index, hairpin ligation adaptor index and read 2 end-coordinate (that is, reads with identical UMI, reverse transcription index, ligation adaptor index and tagmentation site were considered duplicates).
All low-quality reads were removed from the data (including jaws, tooth germs and salivary glands samples from all time points) by setting UMI cutoff to 100 and removing all mitochondrial reads. Following Monocle3 workflow, data underwent normalization by size factor, preprocessing, dimension reduction (UMAP algorithm), and unsupervised graph-based clustering analysis (Leiden Algorithm). Certain clusters from the initial analysis were selected for further sub-clustering, and the previous analysis repeated. Pseudotime analysis also was done with Monocle3 following the default workflow, which include learning the graph, ordering the cells, and plotting the trajectory over UMAP. Mutual nearest neighbors (MNNs) algorithm was used for patch effect correction only between day10 differentiation sample and day16 sample. PanglaoDB, a curated single cell gene expression database was utilized to explore the consensus of cell type markers used across publicly available single cell datasets.
Genome_build: GRCh38-primary-assembly, gencode.v27
Supplementary_files_format_and_content: processed files contain cluster annotations, gene annotations in csv fromat. And the count matrix is in MatrixMarket format.

molecule: polyA RNA

source_name: Induced pluripotent stem cells

growth_protocol: cells were grown in mTeSR1 stem cell media

extract_protocol:
Frozen tissues were carefully transferred to a stack of chilled aluminum foil kept on dry ice to prevent thawing. The folded foil encapsulating the tissues were placed on a block of dry ice and the foil was pounded with a pestle to pulverize the tissues into powder. 1mL of lysis buffer that contains nuclei buffer (10 mM Tris-HCl, 10 mM NaCl, 3 mM MgCl2, pH 7.4), 0.1% IGEPAL CA-630, 1% SUPERase In RNase inhibitor (20 U/μL, Thermo), and 1% BSA (20 mg/mL, NEB) were added onto the tissue powder and transferred to a 1.5mL tubes. Samples were incubated in the lysis buffer for 1 hour on ice. The samples were pipetted up and down with pre-cut 1000uL pipette tip to further disassociate the tissue. The dissociated tissues were passed through 70 um cell strainers (Corning) into a 50mL conical tube. The strainers were rinsed with lysis buffer to minimize nuclei loss. The samples were centrifuged to pellet the nuclei at 500g for 5 minutes at 4°C and the supernatant was discarded. The samples resuspended again in 1ml lysis buffer, transferred into new 15mL tubes, pelleted again and supernatant was discarded. The pellets were resuspended in 50ul of nuclei buffer, and 5 mL of 4% Paraformaldehyde (PFA) (EMS) diluted in RNase free PBS, was added to fix the nuclei for 15 minutes on ice. The tubes were flicked gently every 5 minutes to reduce clumping of nuclei. The fixed nuclei were pelleted at 500g for 3 minutes at 4°C and the PFA waste was discarded. The pelleted nuclei washed in nuclei wash buffer (cell lysis buffer without IGEPAL) and then centrifuged again at 500g for 5 minutes 4°C, and the supernatant was discarded. Finally, the pellets were resuspended again in nuclei wash buffer and then flash-frozen in liquid nitrogen before storing in –80°C.
For nuclei extraction from the differentiation culture, the cells were treated with StemPro Accutase (Theromo, #A1110501) for 7min to detach the cells and transfer them into 15mL tube, then incubated in trypsin (Theromo, #25300054) for another 7min to prevent re-clumping. The cells were span down to remove trypsin after inactivation with more media. The pellet was treated with nuclei lysis buffer and the same steps for nuclei extraction protocol were followed.
Single-cell combinatorial-indexing RNA-sequencing (sci-RNA-seq) protocol is described previously and more details can be found in this link (https://github.com/bbi-lab/). sci-RNA-seq relies on the following steps, (i) thawed nuclei were permeabilized with 0.2% TritonX-100 (Sigma, #T9284) (in nuclei wash buffer) for 3 min on ice, and briefly sonicated to reduce nuclei clumping; (ii) nuclei distributed across 96-well plates; (iii) A first molecular index is introduced to the mRNA of cells within each well, with in situ reverse transcription (RT) incorporating the unique molecular identifiers (UMIs); (iv) All cells were pooled and redistributed to multiple 96-well plates in limiting numbers (e.g., 10 to 100 per well) and a second molecular index is introduced by hairpin ligation;(v) Second strand synthesis, tagmentation, purification and indexed PCR; (vi) Library purification and sequencing is performed.

treatment_protocol: Briefly, hiPSCs (WTC-11 human induced pluripotent stem cells) (Coriell, #GM25256) were seeded on 12-well plates coated with growth factor-reduced Matrigel (Corning) and cultured in mTeSR1 stem cell medium (stemcell, #85850) until cells reach confluency with medium changes daily. On the first day of differentiation (deemed Day 0), stem cell media is replaced with a base media consisted of RPMI 1640 Medium (Thermo, #11875093) mixed with EpiLife (Thermo, #MEPI500CA) at 1:1 ratio, supplemented with 0.1x supplement S7 (Thermo, #S0175), 0.1uM β-mercaptoethanol (BME) (Sigma, #M7522) and 400um smoothened agonist (SAG) (Selleckchem, # S7779). At day 3 of differentiation 150pM of bone morphogenic protein-4 (BMP4) (rndsystems, #314-BP-010) is continuously added daily till day 7. At day 8, the base media is supplemented with 1uM of BMP-I inhibitor (LDN-193189)(Tocris, # 6053), 5uM of GSK3-Inhibitor (CHIR99021) (Selleckchem, # 4423), 500pM epidermal growth factor (EGF) (rndsystems, #236-EG) and 3.5μM of Neurotrophin-4 (NT4) (rndsystems, #268-N4). The cultures were then harvested at day 10 at an oral epithelium stage, or extended to day 16 by adding 300pM BMP4, and 800nM transforming growth factor beta 1(TGF-β1) (rndsystems, #7754-BH) for the pre-ameloblast stage at day16.

value: Induced pluripotent stem cell

tag: age group

value: Ameloblast differentation protocol

tag: experiment type

value: sci-RNA-seq


title: G3550


title: Y3552


title: Retina tissue, KO animal, room air exposed, GSD302

description:
GSD302
column 3 retina_counts.txt
retina_counts.txt


title: Retina tissue, KO animal, room air exposed, GSD301

description:
GSD301
column 2 retina_counts.txt
retina_counts.txt


title: Day10_OE_diff


title: SMSG_17_19w

source_name: Fetal submandibular salivary gland

value: Fetal submandibular salivary gland


title: Chicken spleens from infection group (b2)


title: Lung tissue, KO animal, room air exposed, GSD497

description:
GSD497
column 13 lung_counts.txt
lung_counts.txt

source_name: lung

value: lung


title: W3169


title: Chicken spleens from infection group (b1_2)

value: Technical repetition


title: G3068


title: SMSG_14_16w


title: SMSG_12_13w


title: G3121


title: Chicken spleens from infection group (b1_1)

value: Spleen


title: TERT promoter WT type primary sphere [Parental_P1]

value: human iPSC derived NPC derived tumor

value: TERT promoter WT type primary sphere


title: Lung tissue, KO animal, room air exposed, GSD496

description:
GSD496
column 12 lung_counts.txt
lung_counts.txt

value: GSDMD KO


title: Chicken spleens from control group (a9)

value: white leghorn

value: SPF

value: Control

value: Biological repetition


title: Molars_20_22w

source_name: Fetal tooth germ

value: Fetal molar tooth germ


title: Chicken spleens from infection group (b3)

value: E. coli O78


title: Lung tissue, WT animal, hyperoxia exposed, GSD406

description:
GSD406
column 9 lung_counts.txt
lung_counts.txt

value: WT

value: Hyperoxia


title: G3030

value: BRD


title: Y3059


title: Molars_17_19w

value: sci-RNA-seq


title: Chicken spleens from control group (a8)


title: Molars_12_13w


title: Chicken spleens from control group (a7)


title: Y2904


title: Lung tissue, WT animal, hyperoxia exposed, GSD405

description:
GSD405
column 8 lung_counts.txt
lung_counts.txt


title: Chicken spleens from control group (a6)


title: Incisors_20_22w


title: O2608


title: Lung tissue, WT animal, hyperoxia exposed, GSD403

description:
GSD403
column 7 lung_counts.txt
lung_counts.txt


title: G2500


title: Incisors_17_19w

value: Fetal incisor tooth germ


title: Chicken spleens from control group (a5)


title: Chicken spleens from control group (a4)


title: Lung tissue, KO animal, hyperoxia exposed, GSD493

description:
GSD493
column 11 lung_counts.txt
lung_counts.txt

value: lung


title: Incisors_Molars_14_16w

value: Fetal incisor and molar tooth germ


title: G1979


title: Lung tissue, WT animal, room air exposed, GSD400

description:
GSD400
column 6 lung_counts.txt
lung_counts.txt

value: Room air


title: Lung tissue, WT animal, room air exposed, GSD399

description:
GSD399
column 5 lung_counts.txt
lung_counts.txt


title: Incisors_12_13w


title: Lung tissue, KO animal, room air exposed, GSD490

description:
GSD490
column 10 lung_counts.txt
lung_counts.txt


title: Lung tissue, WT animal, room air exposed, GSD398

description:
GSD398
column 4 lung_counts.txt
lung_counts.txt


title: Chicken spleens from control group (a3)

value: Spleen


title: R119


title: Chicken spleens from control group (a2)


title: Posterior_jaw_9_11w

source_name: Fetal jaw

value: Fetal jaw


title: R1156


title: Chicken spleens from control group (a1_1)

value: Technical repetition


title: R1111


title: Anterior_jaw_9_11w_rep2


title: Lung tissue, KO animal, hyperoxia exposed, GSD061

description:
GSD061
column 2 lung_counts.txt
lung_counts.txt

value: GSDMD KO


title: day2_SM_400

status: Public on Jan 10 2023

city: Kyoto city

first: Ryosuke

last: Yamamoto

state: Kyoto pref

address: 54 Kawahara-cho

department: Department of Otolaryngology, Head and Neck Surgery

country: Japan

institute: Kyoto University

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW40

data_processing:
Demultiplexing: C1 mRNA Seq HT Demultiplex Script v2.0 (Fluidigm)
Mapping: STAR v2.7.0a
Gene counting: SUBREAD v1.6.3
Normalization: Transcripts per million (TPM), quantile normalization (quminorm v0.1.0), sctransform (sctransform v0.3.3)
Assembly: GRCg6a, GCF_000002315.6
Supplementary files format and content: raw_count_file.txt: Unfiltered, non-normalized count file
Supplementary files format and content: normalized_count_file.txt: Filtered, normalized count file (TPM, quantile normalization)
Supplementary files format and content: normalized_seurat_obj.rds: Filtered, normalized Surat object file  (TPM, quantile normalization, scTransform)

source_name: basilar papillae

extract_protocol:
BP specimens were incubated in prewarmed 500 µg/ml thermolysin in medium 199 at 37℃ for 30 min. To stop enzymatic dissociation, specimens were transferred into medium 199 supplemented with 5% FBS. Afterwards, the BP was separated from the surrounding tissues under the stereomicroscope. Then, the samples were transferred into an Eppendorf tube by a pipette. After centrifugation (800g x 5 min) at room temperature, the supernatant was removed. Samples were incubated in an enzyme cocktail containing 50% Accutase and 50% TrypLE Select enzyme for 7 min at 37℃. After gently pipetting 10-20 times, samples were incubated for additional 4 min at 37℃. After inactivation of enzymatic activity with medium 199 supplemented with 5% FBS, further mechanical dissociation by pipetting was performed. Dissociated cells were collected after centrifugation and resuspended in medium 199 supplemented with 5% FBS.
Capture of the single cells and cDNA synthesis were conducted using C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2 (Fluidigm), according to manufacturer’s instruction. Briefly, 12,000 cells of each sample were loaded onto HT IFC, and 400 cells were captured in the IFC. Sequencing libraries were prepared by transposase-assisted tagmentation and enrichment of 3’end of genes using Nextera XT DNA Library Prep Kit (Illumina). The libraries were sequenced on the HiSeq2500 for 25 and 75 cycles paired-end reads.

treatment_protocol: Basilar papillae (BPs) were dissected from the temporal bones (intact) and incubated in the control medium for 24 h (day0_pre). BPs were cultured with 78 microM stereptomycin for 48 h (day2_SM). A part of BPs were maintained additional 96 h in the control medium (day6_SM). Other BPs were cultured in the control medium for 48 h (day2_ctrl) or 144 h (day6_ctrl)

value: basilar papillae

value: 48-h exposure to SM


title: day2_SM_398

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW38


title: day2_SM_395

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW35


title: day2_SM_393

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW33


title: day2_SM_394

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW34


title: day2_SM_391

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW31


title: day2_SM_397

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW37


title: day2_SM_399

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW39


title: Lung tissue, KO animal, hyperoxia exposed, GSD097

description:
GSD097
column 3 lung_counts.txt
lung_counts.txt


title: day2_SM_388

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW28


title: day2_SM_387

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW27


title: Chicken spleens from control group (a1_2)

value: white leghorn

value: SPF

value: Control


title: day2_SM_386

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW26


title: R1008


title: day2_SM_396

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW36


title: day2_SM_390

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW30


title: day2_SM_392

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW32


title: day2_SM_385

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW25


title: Anterior_jaw_9_11w_rep1


title: day2_SM_383

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW23


title: day2_SM_384

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW24


title: day2_SM_379

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW19


title: day2_SM_380

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW20


title: day2_SM_378

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW18


title: day2_SM_381

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW21


title: day2_SM_376

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW16


title: day2_SM_375

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW15


title: day2_SM_377

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW17


title: day2_SM_382

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW22


title: day2_SM_374

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW14


title: day2_SM_389

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW29


title: day2_SM_373

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW13


title: day2_SM_372

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW12


title: day2_SM_370

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW10


title: day2_SM_369

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW09


title: day2_SM_365

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW05


title: day2_SM_364

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW04


title: day2_SM_366

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW06


title: day2_SM_368

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW08


title: day2_SM_362

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW02


title: day2_SM_363

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW03


title: day2_SM_356

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW36


title: day2_SM_354

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW34


title: day2_SM_357

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW37


title: day2_SM_353

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW33


title: day2_SM_358

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW38


title: day2_SM_371

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW11


title: day2_SM_359

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW39


title: day2_SM_355

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW35


title: day2_SM_361

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW01


title: day2_SM_367

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC516_ROW07


title: day2_SM_360

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW40


title: day2_SM_352

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW32


title: day2_SM_344

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW24


title: day2_SM_350

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW30


title: day2_SM_349

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW29


title: day2_SM_340

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW20


title: day2_SM_345

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW25


title: day2_SM_343

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW23


title: day2_SM_346

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW26


title: day2_SM_336

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW16


title: day2_SM_337

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW17


title: day2_SM_338

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW18


title: day2_SM_335

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW15


title: day2_SM_347

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW27


title: day2_SM_341

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW21


title: day2_SM_348

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW28


title: day2_SM_342

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW22


title: day2_SM_351

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW31


title: day2_SM_334

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW14


title: day2_SM_333

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW13


title: day2_SM_326

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW06


title: day2_SM_324

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW04


title: day2_SM_328

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW08


title: day2_SM_331

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW11


title: day2_SM_339

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW19


title: day2_SM_332

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW12


title: day2_SM_330

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW10


title: day2_SM_322

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW02


title: day2_SM_325

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW05


title: day2_SM_320

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW40


title: day2_SM_316

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW36


title: day2_SM_323

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW03


title: day2_SM_314

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW34


title: day2_SM_317

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW37


title: day2_SM_319

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW39


title: day2_SM_318

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW38


title: day2_SM_315

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW35


title: day2_SM_312

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW32


title: day2_SM_313

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW33


title: day2_SM_311

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW31


title: day2_SM_305

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW25


title: day2_SM_310

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW30


title: day2_SM_309

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW29


title: day2_SM_306

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW26


title: day2_SM_307

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW27


title: day2_SM_321

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW01


title: day2_SM_327

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW07


title: day2_SM_329

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC515_ROW09


title: day2_SM_304

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW24


title: day2_SM_302

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW22


title: day2_SM_300

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW20


title: day2_SM_299

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW19


title: day2_SM_308

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW28


title: day2_SM_298

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW18


title: day2_SM_297

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW17


title: day2_SM_301

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW21


title: day2_SM_294

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW14


title: day2_SM_291

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW11


title: day2_SM_293

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW13


title: day2_SM_292

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW12


title: day2_SM_303

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW23


title: day2_SM_290

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW10


title: day2_SM_286

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW06


title: day2_SM_288

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW08


title: day2_SM_295

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW15


title: day2_SM_289

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW09


title: day2_SM_283

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW03


title: day2_SM_284

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW04


title: day2_SM_282

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW02


title: day2_SM_281

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW01


title: day2_SM_279

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW39


title: day2_SM_287

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW07


title: day2_SM_296

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW16


title: day2_SM_277

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW37


title: day2_SM_278

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW38


title: day2_SM_285

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC514_ROW05


title: day2_SM_275

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW35


title: day2_SM_280

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW40


title: day2_SM_274

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW34


title: day2_SM_270

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW30


title: day2_SM_271

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW31


title: day2_SM_272

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW32


title: day2_SM_273

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW33


title: day2_SM_267

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW27


title: day2_SM_268

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW28


title: day2_SM_265

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW25


title: day2_SM_263

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW23


title: day2_SM_269

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW29


title: day2_SM_266

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW26


title: day2_SM_261

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW21


title: day2_SM_259

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW19


title: day2_SM_276

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW36


title: day2_SM_260

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW20


title: day2_SM_255

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW15


title: day2_SM_254

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW14


title: day2_SM_258

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW18


title: day2_SM_257

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW17


title: day2_SM_256

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW16


title: day2_SM_262

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW22


title: day2_SM_250

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW10


title: day2_SM_249

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW09


title: day2_SM_252

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW12


title: day2_SM_251

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW11


title: day2_SM_247

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW07


title: day2_SM_248

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW08


title: day2_SM_246

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW06


title: day2_SM_245

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW05


title: day2_SM_243

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW03


title: day2_SM_241

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW01


title: day2_SM_242

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW02


title: day2_SM_244

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW04


title: day2_SM_239

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW39


title: day2_SM_264

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW24


title: day2_SM_253

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC513_ROW13


title: day2_SM_236

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW36


title: day2_SM_233

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW33


title: day2_SM_232

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW32


title: day2_SM_231

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW31


title: day2_SM_230

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW30


title: day2_SM_227

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW27


title: day2_SM_226

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW26


title: day2_SM_224

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW24


title: day2_SM_223

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW23


title: day2_SM_222

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW22


title: day2_SM_220

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW20


title: day2_SM_221

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW21


title: day2_SM_219

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW19


title: day2_SM_218

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW18


title: day2_SM_238

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW38


title: day2_SM_216

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW16


title: day2_SM_217

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW17


title: day2_SM_240

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW40


title: day2_SM_215

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW15


title: day2_SM_214

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW14


title: day2_SM_237

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW37


title: day2_SM_235

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW35


title: day2_SM_211

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW11


title: day2_SM_210

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW10


title: day2_SM_234

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW34


title: day2_SM_228

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW28


title: day2_SM_209

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW09


title: day2_SM_212

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW12


title: day2_SM_229

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW29


title: day2_SM_208

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW08


title: day2_SM_225

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW25


title: day2_SM_207

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW07


title: day2_SM_206

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW06


title: day2_SM_201

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW01


title: day2_SM_213

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW13


title: day2_SM_200

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW40


title: day2_SM_205

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW05


title: day2_SM_199

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW39


title: day2_SM_198

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW38


title: day2_SM_204

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW04


title: day2_SM_202

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW02


title: day2_SM_196

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW36


title: day2_SM_197

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW37


title: day2_SM_194

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW34


title: day2_SM_193

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW33


title: day2_SM_191

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW31


title: day2_SM_192

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW32


title: day2_SM_190

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW30


title: day2_SM_195

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW35


title: day2_SM_188

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW28


title: day2_SM_189

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW29


title: day2_SM_184

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW24


title: day2_SM_183

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW23


title: day2_SM_186

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW26


title: day2_SM_182

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW22


title: day2_SM_203

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC512_ROW03


title: day2_SM_187

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW27


title: day2_SM_181

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW21


title: day2_SM_185

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW25


title: day2_SM_180

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW20


title: day2_SM_178

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW18


title: day2_SM_179

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW19


title: day2_SM_175

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW15


title: day2_SM_173

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW13


title: day2_SM_174

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW14


title: day2_SM_170

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW10


title: day2_SM_176

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW16


title: day2_SM_167

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW07


title: day2_SM_169

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW09


title: day2_SM_172

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW12


title: day2_SM_166

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW06


title: day2_SM_168

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW08


title: day2_SM_165

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW05


title: day2_SM_171

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW11


title: day2_SM_162

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW02


title: day2_SM_164

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW04


title: day2_SM_158

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW38


title: day2_SM_161

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW01


title: day2_SM_163

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW03


title: day2_SM_159

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW39


title: day2_SM_157

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW37


title: day2_SM_156

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW36


title: day2_SM_155

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW35


title: day2_SM_154

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW34


title: day2_SM_160

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW40


title: day2_SM_148

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW28


title: day2_SM_153

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW33


title: day2_SM_152

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW32


title: day2_SM_150

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW30


title: day2_SM_149

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW29


title: day2_SM_151

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW31


title: day2_SM_147

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW27


title: day2_SM_143

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW23


title: day2_SM_144

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW24


title: day2_SM_145

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW25


title: day2_SM_142

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW22


title: day2_SM_141

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW21


title: day2_SM_177

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC511_ROW17


title: day2_SM_138

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW18


title: day2_SM_140

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW20


title: day2_SM_139

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW19


title: day2_SM_135

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW15


title: day2_SM_137

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW17


title: day2_SM_128

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW08


title: day2_SM_134

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW14


title: day2_SM_132

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW12


title: day2_SM_131

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW11


title: day2_SM_130

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW10


title: day2_SM_126

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW06


title: day2_SM_133

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW13


title: day2_SM_127

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW07


title: day2_SM_125

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW05


title: day2_SM_146

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW26


title: day2_SM_124

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW04


title: day2_SM_122

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW02


title: day2_SM_119

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW39


title: day2_SM_118

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW38


title: day2_SM_120

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW40


title: day2_SM_123

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW03


title: day2_SM_121

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW01


title: day2_SM_136

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW16


title: day2_SM_117

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW37


title: day2_SM_114

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW34


title: day2_SM_109

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW29


title: day2_SM_112

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW32


title: day2_SM_108

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW28


title: day2_SM_111

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW31


title: day2_SM_107

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW27


title: day2_SM_113

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW33


title: day2_SM_129

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC510_ROW09


title: day2_SM_116

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW36


title: day2_SM_110

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW30


title: day2_SM_106

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW26


title: day2_SM_103

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW23


title: day2_SM_104

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW24


title: day2_SM_100

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW20


title: day2_SM_102

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW22


title: day2_SM_101

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW21


title: day2_SM_099

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW19


title: day2_SM_098

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW18


title: day2_SM_097

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW17


title: day2_SM_096

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW16


title: day2_SM_093

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW13


title: day2_SM_091

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW11


title: day2_SM_095

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW15


title: day2_SM_094

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW14


title: day2_SM_090

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW10


title: day2_SM_092

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW12


title: day2_SM_089

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW09


title: day2_SM_088

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW08


title: day2_SM_086

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW06


title: day2_SM_083

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW03


title: day2_SM_084

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW04


title: day2_SM_081

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW01


title: day2_SM_105

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW25


title: day2_SM_082

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW02


title: day2_SM_080

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW40


title: day2_SM_077

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW37


title: day2_SM_075

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW35


title: day2_SM_076

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW36


title: day2_SM_073

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW33


title: day2_SM_074

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW34


title: day2_SM_071

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW31


title: day2_SM_069

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW29


title: day2_SM_068

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW28


title: day2_SM_067

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW27


title: day2_SM_064

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW24


title: day2_SM_065

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW25


title: day2_SM_066

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW26


title: day2_SM_072

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW32


title: day2_SM_062

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW22


title: day2_SM_115

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW35


title: day2_SM_087

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW07


title: day2_SM_070

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW30


title: day2_SM_085

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC509_ROW05


title: day2_SM_061

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW21


title: day2_SM_059

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW19


title: day2_SM_079

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW39


title: day2_SM_078

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW38


title: day2_SM_057

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW17


title: day2_SM_056

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW16


title: day2_SM_055

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW15


title: day2_SM_058

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW18


title: day2_SM_053

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW13


title: day2_SM_052

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW12


title: day2_SM_051

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW11


title: day2_SM_048

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW08


title: day2_SM_049

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW09


title: day2_SM_050

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW10


title: day2_SM_063

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW23


title: day2_SM_046

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW06


title: day2_SM_045

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW05


title: day2_SM_044

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW04


title: day2_SM_043

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW03


title: day2_SM_060

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW20


title: day2_SM_040

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC507_ROW40


title: day2_SM_041

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW01


title: day2_SM_042

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW02


title: day2_SM_054

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC508_ROW14


title: day2_SM_039

description:
C1 Single-Cell mRNA seq HT IFC for medium cell size (10-17 µm) and Reagent kit v2
SC507_ROW39