title: C2C12P_Membrane_Polysome_rep2

description:
C2C12P_Membrane_Polysome_rep2
pA_sites_with_read_counts_C2C12_polysome.csv
Figure 2 , Sample 6


title: C2C12D_Membrane_Polysome_rep1

status: Public on Apr 15 2023

type: SRA

city: Newark

first: Bin

last: Tian

email: btian@wistar.org, dinghaiz@gmail.com

state: NJ

address: 205 South Orange Ave., F-Bay 7

department: Microbiology, Biochemistry and Molecular Genetics

country: USA

institute: Rutgers University, New Jersey Medical School

description:
C2C12D_Membrane_Polysome_rep1
pA_sites_with_read_counts_C2C12_polysome.csv
Figure 2 , Sample 4

library_source: transcriptomic

data_processing:
3'READS+: Sequenced reads (R1) were trimmed to remove adaptor sequence and low-quality sequence (at the 3' end of the reads) using cutadapt (version 2.6) using the following command arguments: -a NNNNTGGAATTCTCGGGTGCCAAGG -n 1 -O 10 -m 25 -f fastq -q 10
3'READS+: Random nucleotides and 5' T-stretches were removed using a custom python script.
3'READS+: The trimmed reads were mapped to the genome using STAR (version 2.7.3a) using the following command arguments: --alignEndsType EndToEnd --outFilterType BySJout --outFilterMultimapNmax 10 --alignSJoverhangMin 8 --alignSJDBoverhangMin 1 --outSAMmultNmax 1 --alignIntronMin 20 --alignIntronMax 1000000.
3'READS+: Reads with a mapping quality score (MAPQ) of ≥10 and ≥2 non-genomic 5'-Ts after alignment (called polyA site supporting (PASS) reads) were identified using a custom python script.
3'READS+: PASS reads with 5' ends (corresponding to the 3' end of 3'UTRs) within 24 nt from one another were clustered into pA sites using a custom python script.
RNA-seq: Sequenced reads (R1 and R2) were trimmed to remove adaptor sequence using cutadapt (version 2.6) using the following command arguments: -a AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -n 1 -O 10 -m 25 -A AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT -f fastq -q 20
RNA-seq:  The trimmed reads were mapped to the genome using STAR (version 2.7.3a) using the following command arguments: --alignEndsType Local --outFilterType BySJout --outFilterMultimapNmax 10 --alignSJoverhangMin 8 --alignSJDBoverhangMin 1 --outSAMmultNmax 1 --alignIntronMin 20 --alignIntronMax 1000000
Genome_build: mm9
Supplementary_files_format_and_content: csv files include all cluster sites (chromosome, strand, position) with count values for each sample.
RNAseq_counts_in_cUTRs_and_aUTRs.csv: RNAseq read counts
pA_sites*csv: 3'READS+ clusters with read counts

molecule: total RNA

organism: Mus musculus

source_name: Differentiating C2C12 Cells

growth_protocol: DMEM supplemented with 2% horse serum, 100 IU/ml penicillin, and 100 μg/ml streptomycin.

extract_protocol:
Phenol/Choloroform
RNAseq libraries: 250 ng of total RNA was used for library construction using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina®.
3'READS+ libraries: The READS+ procedure has been previously described (Zheng et al., 2016). Briefly, polyA+ RNA in 0.1 ~ 2.5 µg of total-cell RNA was captured using 10 µl of oligo(dT)25 magnetic beads (NEB) in 100 µl of 1x Binding Buffer (10 mM Tris-Cl pH 7.5, 150 mM NaCl, 1 mM EDTA, and 0.05% TWEEN 20) and fragmented on the beads using 1.5 U of RNase III (NEB) in 30 µl of RNase III Buffer (10 mM Tris-Cl pH 8.3, 60 mM NaCl, 10 mM MgCl2, and 1 mM DTT) for 15 min at 37 °C. After washing away unbound RNA fragments using Binding Buffer, polyA+ fragments were eluted from the beads using TE Buffer (10 mM Tris-Cl, 1 mM EDTA, pH 7.5) and precipitated using ethanol, followed by ligation to 3 pmol of heat-denatured 5'-adapter (5'-CCUUGGCACCCGAGAAUUCCANNNN-3') in the presence of 1 mM ATP, 0.1 µl of SuperaseIn (ThermoFisher Scientific), and 0.25 µl of T4 RNA ligase 1 in a 5 µl reaction at 22 °C for 1 hr. Ligation products were captured using 10 pmol of biotin-T15-(+TT)5 attached to 12 µl of Dynabeads MyOne Streptavidin C1 (ThermoFisher Scientific). After washing with Washing Buffer (10 mM Tris-Cl pH 7.5, 1 mM NaCl, 1 mM EDTA, and 0.05% TWEEN 20), RNA fragments on the beads were incubated with 0.01 U/µl of RNase H (Epicentre) for 30 min at 37 °C in 30 µl of RNase H buffer (50 mM Tris-Cl pH 7.5, 5 mM NaCl, 10 mM MgCl2, and 10 mM DTT). After washing with RNase H Buffer, RNA fragments were eluted from the beads in Elution Buffer (1 mM NaCl, 1 mM EDTA pH7.5, and 0.05% TWEEN 20) at 50 °C, precipitated using ethanol, and then ligated to 3 pmol of heat-denatured 5'-adenylated 3'-adapter (5'-rApp/NNNGATCGTCGGACTGTAGAACTCTGAAC/3ddC-3') using 0.25 µl of T4 RNA ligase 2 (truncated KQ version) for 1 hr at 22 °C in a 5 µl reaction containing 15% PEG 8000 and 0.2 µl of SuperaseIn. Ligation products were precipitated and reverse-transcribed using M-MLV reverse transcriptase, and PCR-amplified using Phusion high-fidelity DNA polymerase (NEB) and bar-coded PCR primers (Sigma-Aldrich) for 15 cycles. Both RT primer (5'-GTTCAGAGTTCTACAGTCCGACGATC-3') and PCR primers with indexes have been described previously (Hoque et al., 2013). PCR products were size-selected twice using 0.6 volume of AMPure XP beads (Beckman Coulter) relative to the PCR reaction volume to remove large DNA molecules, followed by an additional 0.4 volume of beads to remove small DNA molecules. The eluted DNA was selected again with 1 volume of AMPure XP beads to further remove small DNA molecules. The sizes of the libraries eluted from the AMPure beads were examined using a high-sensitivity DNA kit (Agilent) and an Agilent Bioanalyzer. Library concentrations were measured using the Qubit dsDNA HS Assay Kit (ThermoFisher Scientific). Equal amounts of libraries were mixed and sequenced using an Illumina NextSeq 500 (1x75 bases).

tag: cell type

tag: status

value: Differentiating

tag: cell fraction

value: Membrane Polysome

tag: protocol


title: C2C12D_Cytosol_Polysome_rep1

description:
C2C12D_Cytosol_Polysome_rep1
pA_sites_with_read_counts_C2C12_polysome.csv
Figure 2 , Sample 3

value: Cytosol Polysome


title: 18_0M

city: TSUKUBA

first: Masashi

last: Matsuyama

state: IBARAKI

address: 1-1-1 AMAKUBO

department: respiratory medicine

country: Japan

institute: Tsukuba university

data_processing:
FASTQ files were analyzed using CLC Genomics Workbench (Version 10.1.1; Qiagen, Redwood City, CA).
Genome_build: hg19

organism: Homo sapiens

source_name: Whole blood cells

extract_protocol:
Whole blood was collected with PAXgene blood RNA tubes (Qiagen). Silica-membrane-based RNA isolation and purification in a spin-column format was performed using PAXgene Blood RNA kit (Qiagen). Extracted RNA was globin-cleared using the Ambion GLOBINclear kit (Thermo Fisher, Waltham, MA). 500 ng total RNA was rRNA-depleted using NEBNext rRNA Depletion Kit (New England Biolabs) and converted to an Illumina sequencing library using NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs). Libraries were validated with Bioanalyzer (Agilent Technologies) to determine size distribution and concentration. Sequencing was performed by Tsukuba i-Laboratory LLP (Tsukuba, Japan) using NextSeq500 (Illumina) with paired-end 36-base read option. Sequencing reads were mapped on the hg19 human reference genome and quantified using CLC Genomics Workbench (CLC-GW) version 10.1.1 (Qiagen). Differentially expressed genes were identified by filtering according to p-value obtained by Empirical Analysis of DGE tool in CLC-GW.
RNA libraries were prepared for sequencing using standard Illumina protocols

tag: time point

value: at baseline

tag: tissue

value: Whole blood cells

tag: act-responder

value: yes

value: no

tag: gete-responder

tag: ae-responder

tag: disease state

value: severe eosinophilic asthma


title: C2C12P_Membrane_Polysome_rep1

description:
C2C12P_Membrane_Polysome_rep1
pA_sites_with_read_counts_C2C12_polysome.csv
Figure 2 , Sample 2

source_name: Proliferating C2C12 Cells

growth_protocol: DMEM supplemented with 10% fetal bovine serum, 100 IU/ml penicillin, and 100 μg/ml streptomycin.

value: Proliferating


title: 12_0M


title: C2C12P_Cytosol_Polysome_rep1

description:
C2C12P_Cytosol_Polysome_rep1
pA_sites_with_read_counts_C2C12_polysome.csv
Figure 2 , Sample 1


title: DJ, LP

city: Yangzhou

first: Mingnan

last: Qu

state: Jiangsu

address: Pazhou Dadao Rd, 17-19

country: China

institute: Yangzhou Univerisity

description: DJ+dgp1+glk1glk2 (CK+LP).xlsx

data_processing:
CLC Genomics Workbench v 11.0.1
Sequence reads were trimmed for adaptor sequence/low-quality sequence using CLC genomic benchwork (parameter- Quality limit: 0.05)
Trimmed sequence reads were mapped to Oryza MSU7.0 database using CLC genomic benchwork (parameters- mismath cost: 2 insertion cost: 3 deletion cost: 3 length fraction: 0.8 similarity fraction: 0.8)
Read count extraction and normalization were performed using CLC genomic benchwork.
Assembly: Oryza MSU7.0 database
Supplementary files format and content: tab-delimited text files include RPKM values for each Sample.

organism: Oryza sativa

source_name: leaves

growth_protocol: Seedlings were cultivated in glass test tubes using 0.5× Murashige and Skoog (½ MS) solid medium, at 26 °C under a photoperiod of 13h/11h (light/dark).

extract_protocol:
the total RNA was isolated from the leaf tissue using the RNA extraction kit (Huayueyang, China).
The RNA-Seq library construction was performed using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, Ispawich, USA) following the manufacturer’s instructions.

treatment_protocol:
The 2-weaks cultivated seedlings were transferring to low-Pi condition,leaves were harvested at 6 d after the treatment.
The 2-weaks cultivated seedlings were transferring to non-Pi condition,leaves were harvested at 1 d after the treatment.

value: leaves

tag: genotype

value: WT (DJ)

tag: treatment

value: Low-Pi treatment

tag: time

value: 6 day after treatment


title: 11_0M


title: 10_0M


title: DJ, CK

description: DJ+dgp1+glk1glk2 (CK+LP).xlsx

value: Control


title: C2C12D_Membrane_rep1

description:
C2C12D_Membrane_rep1
pA_sites_with_read_counts_C2C12.csv
Figure 1, Sample 7

extract_protocol:
TRIZol
RNAseq libraries: 250 ng of total RNA was used for library construction using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina®.
3'READS+ libraries: The READS+ procedure has been previously described (Zheng et al., 2016). Briefly, polyA+ RNA in 0.1 ~ 2.5 µg of total-cell RNA was captured using 10 µl of oligo(dT)25 magnetic beads (NEB) in 100 µl of 1x Binding Buffer (10 mM Tris-Cl pH 7.5, 150 mM NaCl, 1 mM EDTA, and 0.05% TWEEN 20) and fragmented on the beads using 1.5 U of RNase III (NEB) in 30 µl of RNase III Buffer (10 mM Tris-Cl pH 8.3, 60 mM NaCl, 10 mM MgCl2, and 1 mM DTT) for 15 min at 37 °C. After washing away unbound RNA fragments using Binding Buffer, polyA+ fragments were eluted from the beads using TE Buffer (10 mM Tris-Cl, 1 mM EDTA, pH 7.5) and precipitated using ethanol, followed by ligation to 3 pmol of heat-denatured 5'-adapter (5'-CCUUGGCACCCGAGAAUUCCANNNN-3') in the presence of 1 mM ATP, 0.1 µl of SuperaseIn (ThermoFisher Scientific), and 0.25 µl of T4 RNA ligase 1 in a 5 µl reaction at 22 °C for 1 hr. Ligation products were captured using 10 pmol of biotin-T15-(+TT)5 attached to 12 µl of Dynabeads MyOne Streptavidin C1 (ThermoFisher Scientific). After washing with Washing Buffer (10 mM Tris-Cl pH 7.5, 1 mM NaCl, 1 mM EDTA, and 0.05% TWEEN 20), RNA fragments on the beads were incubated with 0.01 U/µl of RNase H (Epicentre) for 30 min at 37 °C in 30 µl of RNase H buffer (50 mM Tris-Cl pH 7.5, 5 mM NaCl, 10 mM MgCl2, and 10 mM DTT). After washing with RNase H Buffer, RNA fragments were eluted from the beads in Elution Buffer (1 mM NaCl, 1 mM EDTA pH7.5, and 0.05% TWEEN 20) at 50 °C, precipitated using ethanol, and then ligated to 3 pmol of heat-denatured 5'-adenylated 3'-adapter (5'-rApp/NNNGATCGTCGGACTGTAGAACTCTGAAC/3ddC-3') using 0.25 µl of T4 RNA ligase 2 (truncated KQ version) for 1 hr at 22 °C in a 5 µl reaction containing 15% PEG 8000 and 0.2 µl of SuperaseIn. Ligation products were precipitated and reverse-transcribed using M-MLV reverse transcriptase, and PCR-amplified using Phusion high-fidelity DNA polymerase (NEB) and bar-coded PCR primers (Sigma-Aldrich) for 15 cycles. Both RT primer (5'-GTTCAGAGTTCTACAGTCCGACGATC-3') and PCR primers with indexes have been described previously (Hoque et al., 2013). PCR products were size-selected twice using 0.6 volume of AMPure XP beads (Beckman Coulter) relative to the PCR reaction volume to remove large DNA molecules, followed by an additional 0.4 volume of beads to remove small DNA molecules. The eluted DNA was selected again with 1 volume of AMPure XP beads to further remove small DNA molecules. The sizes of the libraries eluted from the AMPure beads were examined using a high-sensitivity DNA kit (Agilent) and an Agilent Bioanalyzer. Library concentrations were measured using the Qubit dsDNA HS Assay Kit (ThermoFisher Scientific). Equal amounts of libraries were mixed and sequenced using an Illumina NextSeq 500 (1x75 bases).

value: Membrane


title: XSJ3

city: 广州

first: huabin

last: xie

state: 广东

address: 广东

institute: 没有

data_processing:
The libraries were sequenced on an Illumina NovaSeq6000 platform and 150 bp paired-end reads were generated.
Raw data (raw reads) of fastq format were firstly processed using Trimmomatic and the low quality reads were removed to obtain the clean reads.
The clean reads were mapped to the IRGSP-1.0 using HISAT2.
FPKM of each gene was calculated using Cufflinks, and the read counts of each gene were obtained by HTSeq-count.
Assembly: IRGSP-1.0
Supplementary files format and content: tab-delimited text files include FPKM values for each Sample

source_name: leaf blade

extract_protocol:
Total RNA was extracted using the TRIzol reagent according to the manufacturer’s protocol.
Then the libraries were constructed using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions.

value: susceptible

value: leaf blade

value: WT

value: 12 hours

value: Inoculate Xoc


title: C2C12P_Cytosol_rep2

description:
C2C12P_Cytosol_rep2
pA_sites_with_read_counts_C2C12.csv
Figure 1&3&4 , Sample 8

value: Cytosol


title: C2C12P_Membrane_rep1

description:
C2C12P_Membrane_rep1
pA_sites_with_read_counts_C2C12.csv
Figure 1, Sample 5


title: C2C12P_Cytosol_rep1

description:
C2C12P_Cytosol_rep1
pA_sites_with_read_counts_C2C12.csv
Figure 1, Sample 4


title: XSJ2


title: 8_0M

value: at baseline

value: Whole blood cells


title: 9_0M

value: yes

value: no


title: C2C12P_Insoluble_RNAseq_rep1

description:
C2C12P_Insoluble_RNAseq_rep1
RNAseq_counts_in_cUTRs_and_aUTRs.csv
Figure 1 , Sample 3

value: Proliferating

value: Insoluble

value: RNAseq


title: C2C12P_Cytosol_RNAseq_rep1

description:
C2C12P_Cytosol_RNAseq_rep1
RNAseq_counts_in_cUTRs_and_aUTRs.csv
Figure 1 , Sample 1


title: XSH3

value: Inoculate PBS


title: 6_0M

value: severe eosinophilic asthma


title: XSH2


title: 5_0M


title: XSH1


title: C2C12P_Membrane_rep2

description:
C2C12P_Membrane_rep2
pA_sites_with_read_counts_C2C12.csv
Figure 1&3&4 , Sample 9


title: C2C12D_Cytosol_rep1

description:
C2C12D_Cytosol_rep1
pA_sites_with_read_counts_C2C12.csv
Figure 1, Sample 6

value: Differentiating


title: 3_0M


title: XKJ2

value: resistant


title: XKJ3


title: 2_0M


title: 1_0M


title: XSJ1

value: susceptible


title: C2C12P_Membrane_RNAseq_rep1

description:
C2C12P_Membrane_RNAseq_rep1
RNAseq_counts_in_cUTRs_and_aUTRs.csv
Figure 1 , Sample 2

value: Membrane


title: 7_0M


title: XKH3

value: leaf blade

value: WT


title: JAPC24_(Celegans)_swan-1;vhl-1_BioRep3

type: RNA

city: Ames

first: Jo Anne

last: Powell-Coffman

state: Iowa

address: 2213 Pammel Dr.

department: Department of Genetics, Development and Cell Biology

institute: Iowa State University

description: Gene expression

hyb_protocol: Samples were hybridized to Affymetrix GeneChip® C. elegans Genome array according to Affymetrix specifications.

scan_protocol: The arrays were scanned with GeneChip® scanner 3000 7G.

data_processing: The Affymetrix® GeneChip® Command Console™ (AGCC) software was used to generate probe cell intensity data (.CEL) files.

label: Biotin

organism: Caenorhabditis elegans

source_name: swan-1(ok267);vhl-1(ok161) loss-of-function double mutants

label_protocol: The total RNA was synthesized to biotin-labeled aRNA using the GeneChip® 3' IVT Express Kit (Affymetrix, part number 901229).

growth_protocol: Worms were grown on NGM plates seeded with OP50 at 21°C.

extract_protocol: The total RNA isolation was performed using Trizol (Invitrogen) and RNeasy Mini Kit (Qiagen).

treatment_protocol: The hypoxia treatment was 0.5% oxygen for 2 hours.

value: Untreated


title: JAPC20_(Celegans)_egl-9_BioRep2

description: Gene expression

source_name: egl-9(sa307) loss-of-function mutants


title: XKJ1

value: 12 hours

value: Inoculate Xoc


title: JAPC23_(Celegans)_swan-1;vhl-1_BioRep2

description: Gene expression


title: JAPC16_(Celegans)_rhy-1_BioRep1

description: Gene expression

source_name: rhy-1(ok1402) loss-of-function mutants


title: JAPC18_(Celegans)_rhy-1_BioRep3

description: Gene expression


title: JAPC17_(Celegans)_rhy-1_BioRep2

description: Gene expression


title: JAPC22_(Celegans)_swan-1;vhl-1_BioRep1

description: Gene expression


title: 4_0M

value: at baseline

value: Whole blood cells


title: XKH1


title: JAPC21_(Celegans)_egl-9_BioRep3

description: Gene expression


title: JAPC14_(Celegans)_vhl-1_BioRep2

description: Gene expression

source_name: vhl-1(ok161) loss-of-function mutants


title: JAPC15_(Celegans)_vhl-1_BioRep3

description: Gene expression


title: JAPC12_(Celegans)_hif-1 hypoxia_BioRep3

description: Gene expression

source_name: hif-1(ia04) loss-of-function mutants

value: Hypoxia


title: NP, input

library_source: genomic

data_processing:
Basecalls were performed using bcl2fastq v2.17 for Novaseq output.
ChIP-seq reads were trimmed from 3' end until the final base had a quality score > 30, using Trimmomatic v0.33, discarding reads left with < 20 bp
ChIP-seq reads were aligned to the UCSC hg38 genome using Bowtie version 1.1.2. Only uniquely mapping reads with at most two mismatches were retained.
To create ChIP-seq coverage plots, the locations of the mapped ChIP-seq reads were extended to 150 bp to represent sequenced fragments, renormalized (to reads per million, rpm) and reformatted in the bigWig file format.
Peaks were called uing MACS v2.1.0 with the significance cut-off q-value <=0.01
bigWig files were generated using the genomicRanges R package. Score represents the normalized coverage of DNA fragments at a given genomic coordinate. narrowPeak files were generated using MACS v2 with default settings.
Assembly: Oryza MSU7.0 database
Supplementary files format and content: bigWig, narrowPeak (except for Input sample)

molecule: genomic DNA

source_name: Pi-starved leaves

growth_protocol: Seedlings were cultivated in 0.5× Murashige and Skoog (½ MS) solid medium, at 26 °C under a photoperiod of 13h/11h (light/dark).

extract_protocol:
Leaves were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a E220 focused-ultrasonicator (Covaris).
ChIP-seq libraries were prepared using the KAPA HTP Library Preparation Kit complemented with NEXTflex DNA Barcodes from Bioo Scientific. 10 ng of DNA was used as starting material for input and ip samples. Libraries were amplified using 13 cycles on the thermocycler. Post amplification libraries were size selected at 250-450bp in length using Agencourt AMPure XP beads from Beckman Coulter. Libraries were validated using the Agilent High Sensitivity DNA Kit.

treatment_protocol: The 2-weaks cultivated seedlings were transferring to non-phosphorus (non-Pi) condition,leaves were harvested at 3 d after the treatment.

value: Pi-starved leaves

tag: chip antibody

value: input


title: XKH2


title: NP, lgG

value: lgG


title: JAPC11_(Celegans)_hif-1 hypoxia_BioRep2

description: Gene expression


title: JAPC10_(Celegans)_hif-1 hypoxia_BioRep1

description: Gene expression


title: NP, GLK2


title: NP, GLK1


title: WT_veh_Rep4

city: Madison

first: Emery

middle: H

last: Bresnick

address: 1111 Highland Avenue

institute: University of Wisconsin-Madison

data_processing:
RNA-seq fragments were aligned by STAR (version 2.5.2b) to the mouse genome (mm10) with GENCODE basic gene annotations (version M22)
Gene expression levels were quantified by RSEM (version 1.3.0)
Assembly: mm10
Supplementary files format and content: TSV: RSEM quantification results at the gene level

source_name: Fetal liver

growth_protocol: Cells were cultured in OPTI-MEM supplemented with 10% FBS, 1% penicillin-streptomycin, 1% SCF-conditioned medium, 30 uM 2-mercaptoethanol, 1 uM b-estradiol, and 500 ug/mL G418

extract_protocol:
RNA was isolated using TRIzol and purified using RNeasy MinElute Cleanup Kit (Qiagen, 74204).
The RNA library was prepared with Illumina TruSeq Stranded Total RNA (rRNA reduction).

treatment_protocol: 250,000 cells were treated with vehicle, IFNg (1 ng/ml), Pam3CSK4 (100 ng/ml), or combination of agonists for 4 h.

value: Fetal liver

tag: cell line

value: Hematopoietic progenitor

value: Vehicle


title: JAPC08_(Celegans)_hif-1_BioRep2

description: Gene expression


title: WT_veh_Rep3


title: JAPC19_(Celegans)_egl-9_BioRep1

description: Gene expression


title: JAPC13_(Celegans)_vhl-1_BioRep1

description: Gene expression


title: SMG_WKY1-5

city: Beijing

first: Jinghua

last: Yang

address: 32 North Third Ring Road West, Haidian District, Beijing

institute: Beijing Zhiyun data technology co. LTD

hyb_protocol: 100 μl of hybridization solution was dispensed into the gasket slide and assembled to the gene expression microarray slide. The slides were incubated for 17 hours at 65°C in an Agilent Hybridization Oven. The hybridized arrays were washed, fixed and scanned with using the Agilent DNA Microarray Scanner (part number G2505C).

scan_protocol: The hybridized arrays were washed, fixed and scanned using the Agilent DNA Microarray Scanner .Scanned images were imported into Agilent Feature Extraction software for raw data extraction. Agilent Feature Extraction software (version 11.0.1.1) was used to analyze acquired array images.

data_processing: Quantile normalization and subsequent data processing were performed with using the GeneSpring GX v12.1 software package (Agilent Technologies). After quantile normalization of the raw data, LncRNAs and mRNAs that at least 5 out of 10 samples have flags in Detected (“All Targets Value”) were chosen for further data analysis. Differentially expressed genes with statistical significance between the two groups were identified through Volcano Plot filtering. Differentially expressed genes between the two samples were identified through Fold Change filtering. Hierarchical Clustering was performed using the R scripts. GO analysis and Pathway analysis were performed in the standard enrichment computation method.

organism: Rattus norvegicus

source_name: submandibular gland, WKY

label_protocol: Sample labeling and array hybridization were performed according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology). Briefly, total RNA from each sample was linearly amplified and labeled with Cy3-UTP. The labeled cRNAs were purified by RNeasy Mini Kit (Qiagen). The concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000. 1 μg of each labeled cRNA was fragmented by adding 11 μl 10 × Blocking Agent and 2.2 μl of 25 × Fragmentation Buffer, then heated the mixture at 60°C for 30 min, finally 55 μl 2 × GE Hybridization buffer was added to dilute the labeled cRNA.

extract_protocol: Total RNA was extracted using Trizol reagent (Invitrogen Life Technologies, Carlsbad, CA, USA) according to the manufacturer’s instructions.

value: submandibular gland

tag: strain

value: Wistar Kyoto (WKY)

tag: gender

value: male

tag: age

value: 14 week


title: JAPC06_(Celegans)_N2 hypoxia_BioRep3

description: Gene expression

source_name: N2 wild type


title: JAPC05_(Celegans)_N2 hypoxia_BioRep2

description: Gene expression


title: CK, input

tag: cell type

value: leaves


title: SMG_WKY4-2


title: SMG_WKY1-3


title: JAPC04_(Celegans)_N2 hypoxia_BioRep1

description: Gene expression


title: SMG_WKY4-1


title: JAPC09_(Celegans)_hif-1_BioRep3

description: Gene expression

value: Untreated


title: WT_veh_Rep2


title: SMG_WKY1-2


title: JAPC07_(Celegans)_hif-1_BioRep1

description: Gene expression


title: CK, lgG


title: WT_veh_Rep1


title: WT_pam3_Rep4


title: JAPC02_(Celegans)_N2_BioRep2

description: Gene expression


title: SMG_SHR3-3

source_name: submandibular gland, SHR

value: Spontaneously Hypertensive (SHR)


title: SMG_SHR3-4


title: WT_pam3_Rep3


title: SMG_SHR3-1


title: WT_pam3_Rep2


title: CK, GLK2


title: SMG_SHR2-3


title: WT_pam3_Rep1


title: CK, GLK1


title: JAPC03_(Celegans)_N2_BioRep3

description: Gene expression


title: WT_ifng_Rep4

value: IFNg


title: NIH3T3_RC_Membrane_Polysome_rep1

description:
NIH3T3_RC_Membrane_Polysome_rep1
pA_sites_with_read_counts_NIH3T3_polysome.csv
Figure 7 , Sample 6

source_name: NIH3T3 cells

growth_protocol: DMEM supplemented with 10% calf serum, 100 IU/ml penicillin, and 100 μg/ml streptomycin.

tag: status

value: Proliferating

value: 250 microM of sodium arsenite (NaASO2) for 60 min then 60 min of recovery in normal medium

tag: cell fraction

value: Membrane Polysome


title: WT_ifng_Rep3


title: WT_ifng_Rep2


title: WT_ifng_Rep1


title: NIH3T3_AS_Membrane_Polysome_rep1

description:
NIH3T3_AS_Membrane_Polysome_rep1
pA_sites_with_read_counts_NIH3T3_polysome.csv
Figure 7 , Sample 4

value: 250 microM of Sodium Arsenite for 60 min

tag: protocol


title: JAPC01_(Celegans)_N2_BioRep1

description: Gene expression


title: NIH3T3_RC_Cytosol_Polysome_rep1

description:
NIH3T3_RC_Cytosol_Polysome_rep1
pA_sites_with_read_counts_NIH3T3_polysome.csv
Figure 7 , Sample 5

value: Cytosol Polysome


title: C. elegans, HIF-1a, IP, Bio rep2

data_processing:
The fastq files were provided by the Iowa State University DNA facility. The quality scores of the fastq reads for the input and ChIP DNA samples from both biological replicates were checked and they were all above 30, indicating high quality sequencing data.
Reads were mapped to C. elegans reference genome ce11 using bowtie 2 with the default settings.
Reads with mapping quality (MAPQ) score less than 10 and reads mapped to the mitochondrial genome were excluded for peak calling.
Reads were assigned to bins, the size of which was set at 200 bp. Bin-level read counts were analyzed by R package MOSAiCS (MOdel-based one and two Sample Analysis and Inference for ChIP-Seq Data) to call peaks (https://www.bioconductor.org/packages/release/bioc/manuals/mosaics/man/mosaics.pdf). The false discovery rate (FDR) was set at 0.05. Neighboring peaks were merged. The wiggle files were also generated with this package.
Assembly: ce11
Supplementary files format and content: wig, narrowPeak

source_name: Whole organism

extract_protocol:
Worms were harvested and cross-linked in 2% formaldehyde at 21°C for 30 minutes. Nuclear lysates were sonicated using a Branson sonifer microtip to fragment the chromatin to 200-800 bp. Immunoprecipitated protein-DNA complexes were captured on protein A-Sepharose beads (Sigma) and eluted in elution buffer (1% SDS and 100 mM NaHCO3) at 65°C for 30 minutes. Following RNase treatment and cross-link reversal, the ChIP DNA was purified with the Qiagen MinElute Kit for sequencing in parallel with the corresponding input DNA.
The ChIP-seq library preparation and sequencing were performed by the Iowa State University DNA facility. In brief, NEXTflex™ ChIP-Seq Barcodes kit (Illumina compatible) (BIOO Scientific Corp., cat. no. 514123) was used to prepare multiplexed single-end genomic DNA libraries. The gel slices corresponding to the 200-300 bp maker were cut and purified. The purified DNA was amplified and sequenced in a single flow cell on the IlluminaHiSeq 2000 platform. The length of single-end reads was 50 bp.

tag: tissue

value: Whole organism

value: ChIP grade anti-HA antibody (Abcam, cat. no. ab9110)


title: SMG_SHR2-1


title: circRNA_SMG_WKY4-2

hyb_protocol: The labeled cRNAs were purified by RNeasy Mini Kit (Qiagen). The concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000. 1 μg of each labeled cRNA was fragmented by adding 5 μl 10 × Blocking Agent and 1 μl of 25 × Fragmentation Buffer, then heated the mixture at 60 °C for 30 min, finally 25 μl 2 × Hybridization buffer was added to dilute the labeled cRNA. 50 μl of hybridization solution was dispensed into the gasket slide and assembled to the circRNA expression microarray slide. The slides were incubated for 17 hours at 65 °C in an Agilent Hybridization Oven. The hybridized arrays were washed, fixed and scanned using the Agilent Scanner G2505C.

scan_protocol: The hybridized arrays were washed, fixed and scanned using the Agilent Scanner G2505C.Scanned images were imported into Agilent Feature Extraction software for raw data extraction.

data_processing: Agilent Feature Extraction software (version 11.0.1.1) was used to analyze acquired array images. Quantile normalization and subsequent data processing were performed using the R software limma package. Differentially expressed circRNAs with statistical significance between two groups were identified through Volcano Plot filtering. Differentially expressed circRNAs between two samples were identified through Fold Change filtering. Hierarchical Clustering was performed to show the distinguishable circRNAs expression pattern among samples.

label_protocol: Sample labeling and array hybridization were performed according to the manufacturer’s protocol (Arraystar Inc.) Briefly, total RNAs were digested with Rnase R (Epicentre, Inc.) to remove linear RNAs and enrich circular RNAs. Then, the enriched circular RNAs were amplified and transcribed into fluorescent cRNA utilizing a random priming method (Arraystar Super RNA Labeling Kit; Arraystar).


title: WT_combo_Rep4

value: Fetal liver

value: Hematopoietic progenitor

value: IFNg and Pam3CSK4


title: WT_combo_Rep3


title: C. elegans, HIF-1a, Input, Bio rep2


title: NIH3T3_AS_Cytosol_Polysome_rep1

description:
NIH3T3_AS_Cytosol_Polysome_rep1
pA_sites_with_read_counts_NIH3T3_polysome.csv
Figure 7 , Sample 3


title: C. elegans, HIF-1a, IP, Bio rep1


title: NIH3T3_NT_Membrane_Polysome_rep1

description:
NIH3T3_NT_Membrane_Polysome_rep1
pA_sites_with_read_counts_NIH3T3_polysome.csv
Figure 7 , Sample 2


title: NIH3T3_NT_Cytosol_Polysome_rep1

description:
NIH3T3_NT_Cytosol_Polysome_rep1
pA_sites_with_read_counts_NIH3T3_polysome.csv
Figure 7 , Sample 1


title: circRNA_SMG_WKY1-5

value: submandibular gland

value: Wistar Kyoto (WKY)


title: circRNA_SMG_WKY4-1

value: male

value: 14 week


title: C. elegans, HIF-1a, Input, Bio rep1


title: C2C12D_4sU_rep2

description:
C2C12D_4sU_rep2
pA_sites_with_read_counts_C2C12_4sU.csv
Figure 5 , Sample 8

source_name: Differentiating C2C12 Cells

growth_protocol: Differentiating

value: Differentiating

value: 50 microM of 4sU for 60 min then 60 min in normal medium.


title: WT_combo_Rep1


title: C2C12D_total_rep2

description:
C2C12D_total_rep2
pA_sites_with_read_counts_C2C12_4sU.csv
Figure 5, 6 , Sample 7

value: Total


title: KO_veh_Rep4

value: Vehicle


title: C2C12P_4sU_rep2

description:
C2C12P_4sU_rep2
pA_sites_with_read_counts_C2C12_4sU.csv
Figure 5 , Sample 6

value: Proliferating


title: KO_veh_Rep2


title: C2C12P_total_rep2

description:
C2C12P_total_rep2
pA_sites_with_read_counts_C2C12_4sU.csv
Figure 5, 6 , Sample 5


title: KO_veh_Rep1


title: KO_veh_Rep3


title: WT_combo_Rep2


title: circRNA_SMG_WKY1-3


title: KO_pam3_Rep4


title: circRNA_SMG_WKY1-2


title: circRNA_SMG_SHR3-4

value: Spontaneously Hypertensive (SHR)


title: circRNA_SMG_SHR3-3


title: C2C12D_total_rep1

description:
C2C12D_total_rep1
pA_sites_with_read_counts_C2C12_4sU.csv
Figure 5, 6 , Sample 3


title: INF 03

type: protein

city: Siena

first: Erika

last: Bartolini

state: Italy

address: Via Fiorentina 1

department: AIMB

country: Italy

institute: GSK Vaccines

description:
NCT00937521
00019925_Area3-INF 03

hyb_protocol: Protein arrays were first blocked 60 min in Blocking Buffer (Block it, Arrayit). The array was then incubated 1h a room temperature (RT) with human sera diluted 1:1000 for anti-human IgG-specific analysis. After two washing with 0.1% Tween in PBS buffer (TPBS), arrays were incubated with an AlexaFluor 647-conjugated IgG anti-human (1:800 – Jackson Immunoresearch) at RT for 1 h to detect interactions. Slides were washed again as before and washed once in PBS, then rinsed in sterile water and dried.

scan_protocol: Fluorescence signals were detected by using a Powerscanner (Tecan Trading ) and the 16-bit images were generated with Powerscanner software at 10 µm per pixel resolution.

data_processing:
Images were processed using ImaGene 9.0 software (Biodiscovery Inc, CA, USA). Elaboration and analysis of image raw fluorescence Intensity (FI) data  was performed using in-house developed R scripts. For each protein the fluorescence intensity data of the replicates was subtracted by local background values surrounding each spot and averaged, hence resulting in a single data point per sample named Mean Fluorescence Intensity (MFI).
Mean fluorescence intensity (MFI)

label: AlexaFluor 647-conjugated anti-human IgG secondary antibody

molecule: protein

source_name: Serum-post IV rMenB +25 µg OMV dose

label_protocol: The secondary antibody used for arrays was from Jackson Immunoresearch, (872 W Baltimore Pike, West Grove, PA 19390 USA). It was conjugated to the Alexafluor dye (Alexa 647) by the company.

extract_protocol: Human pool sera (25 subjects) from NCT00937521

tag: subclass

value: Infant

value: Blood

value: post IV rMenB +25 µg OMV dose


title: INF 03-PI

description:
NCT00937521
FS00009245_Area3-INF 03-PI

source_name: Serum-PI

value: PI


title: INF 02

description:
NCT00937521
00019925_Area2-INF 02

source_name: Serum-post IV rMenB +6.25 µg OMV dose

value: post IV rMenB +6.25 µg OMV dose


title: circRNA_SMG_SHR3-1


title: KO_pam3_Rep2

value: Fetal liver

value: Hematopoietic progenitor


title: circRNA_SMG_SHR2-3

value: submandibular gland


title: C2C12P_total_rep1

description:
C2C12P_total_rep1
pA_sites_with_read_counts_C2C12_4sU.csv
Figure 5, 6 , Sample 1

source_name: Proliferating C2C12 Cells


title: INF 02-PI

description:
NCT00937521
FS00009245_Area2-INF 02-PI


title: C2C12D_4sU_rep1

description:
C2C12D_4sU_rep1
pA_sites_with_read_counts_C2C12_4sU.csv
Figure 5 , Sample 4


title: KO_pam3_Rep1


title: INF 01

description:
NCT00937521
00019925_Area1-INF 01

source_name: Serum-post IV rMenB dose

value: post IV rMenB dose


title: KO_ifng_Rep4

value: IFNg


title: circRNA_SMG_SHR2-1

value: male

value: 14 week


title: KO_ifng_Rep3


title: KO_pam3_Rep3


title: C2C12P_4sU_rep1

description:
C2C12P_4sU_rep1
pA_sites_with_read_counts_C2C12_4sU.csv
Figure 5 , Sample 2

value: 50 microM of 4sU for 60 min then 60 min in normal medium.


title: KO_ifng_Rep2


title: Z. mobilis ZM4 cells pretreated with cold plasma, Control1, Min4 [S3]

city: Changzhou

first: Xia

last: Yi

address: Gehu Middle Road #21

institute: Changzhou University

description:
genes.fpkm.anno.txt
genes.rawcount.anno.txt

data_processing:
It used HTSeq (high-throughput sequencing) to calculate gene counts of mRNA
It determined the differentially expressed mRNA using DESeq
DAVID (Database for Annotation, Visualization, and Integrated Discovery) and IPA (Ingenuity pathway analysis) software were separately used to analyzed Gene ontology (GO) and KEGG pathway with p_value < 0.05.
Here, it defined differentially expressed genes (DEGs) as log2 ratio ≥ 1.0.
Assembly: NC_006526.2
Supplementary files format and content: tab-delimited text files include RPKM values for each Sample

organism: Zymomonas mobilis

source_name: Z. mobilis ZM4

growth_protocol: Z. mobilis cells were cultured in RM medium at 30 oC without shaking

extract_protocol:
RNA was harvested using TRIzol® reagent (Invitrogen, Carlsbad, CA, USA). 0.1-1.0 μg of total RNA was used for the construction of sequencing libraries.
RNA libraries for RNA-seq were prepared using NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer's protocols.

treatment_protocol: Z. mobilis cells pretreated with cold plasma at 165 Pa, 20 s and 140 W

value: Z. mobilis ZM4

value: Mutant

value: Pretreated with cold plasma

value: Min 4


title: Y30M_3

city: Lanzhou

first: shaoke

last: guo

state: Gansu

address: West Lake Street

department: Lanzhou institute of husbandry and pharmaceutical sciences

institute: Chinese Academy of Agricultural Sciences

data_processing:
Trimmomatic software was used to trim the raw reads of the generated FASTQ format sequence
BLASTN software was used to compared with the nucleotide library
SortMeRNA software was used to remove ribosomal RNA
HISAT2 software was used to compare clean reads to the yak reference
Guitar R package and deepTools software were used for quality inspection of genome comparison result data
Assembly: LU_Bosgru_v3.0
Supplementary files format and content: tab-delimited text files include FPKM values for each Sample …

organism: Bos grunniens

source_name: testis

growth_protocol: Six healthy male yaks from Xiahe County, Gannan Tibetan Autonomous Prefecture (34° 51′ N, 102° 26′ E), were selected. According to the degree of sexual maturity, three 6-month-old yaks (Y6M) before sexual maturity were selected for the first group, and three 30-month-old yaks (Y30M) were selected for the second group.

extract_protocol:
The total RNA was isolated and purified using the TRIzol reagent . The amount and purity of the RNA samples were quantified using the NanoDrop ND-1000.
The qualified RNA samples were constructed into a cDNA library, and oligo-dT magnetic beads were used to specifically capture mRNA with polyadenylate by two rounds of purification. A magnesium ion interruption kit was used for fragmentation of captured mRNA at 86°C for 7 min. The fragmented mRNA was pre-mixed with the immunomagnetic beads Dynabeads Antibody Coupling Kit  and m6A Antibody  in immunoprecipitation (IP) buffer (50 mM Tris-HCl, 750 mM NaCl, and 0.5% Igepal CA-630) before conducting IP. The IP product was passed through reverse transcriptase . Then E. coli DNA polymerase I was combined with RNase H and mixed with dUTP solution for two-strand synthesis and RNA degradation. AMPure XP beads were used to select the fragment size, and a 300 ± 50 bp library was obtained. Finally, two-terminal sequencing was performed on the Illumina NovaSeq 6000 platform with sequencing mode PE150

treatment_protocol: The testicular parenchyma vertical with the longitudinal axis was collected, and the cut tissue was trimmed into a tissue block with a volume of about 1 cm3, which was fixed in 10% neutral formaldehyde for producing tissue sections, and the consistency of the collection site was maintained.

value: testis

tag: developmental stage

value: after  sexual maturity


title: KO_ifng_Rep1


title: INF 01-PI

description:
NCT00937521
FS00009245_Area1-INF 01-PI

value: Infant

value: Blood

value: PI (pre-immune treatment)


title: Z. mobilis ZM4 cells pretreated with cold plasma, Control1, Min4 [S2]


title: Y30M_2


title: KO_combo_Rep4

value: Fetal liver

value: IFNg and Pam3CSK4


title: Z. mobilis ZM4 cells pretreated with cold plasma, Control1, Min4 [S1]


title: Y30M_1


title: KO_combo_Rep3

value: Hematopoietic progenitor


title: Z. mobilis ZM4 cells, Control3, Min4 [C3]

value: WT

value: Untreated with cold plasma


title: KO_combo_Rep2


title: Y6M_2

data_processing:
Trimmomatic software was used to trim the raw reads of the generated FASTQ format sequence
BLASTN software was used to compared with the nucleotide library
SortMeRNA software was used to remove ribosomal RNA
HISAT2 software was used to compare clean reads to the yak reference
Guitar R package and deepTools software were used for quality inspection of genome comparison result data
Assembly: LU_Bosgru_v3.0
Supplementary files format and content: tab-delimited text files include FPKM values for each Sample …
Library strategy: MeRIP-seq

value: before  sexual maturity


title: Z. mobilis ZM4 cells, Control2, Min4 [C2]


title: si-cav2

city: Guangzhou

first: Yijie

last: Zhang

state: Guangdong

address: Nanfang Hospital

institute: Nanfang Hospital

description: Gene expression data from mouse primary hepatic stellate cells

hyb_protocol: 0.6 μg of Cy3-labelled cRNA (specific activity >10.0 pmol Cy3/μg cRNA) was fragmented at 60°C for 30 minutes in a reaction volume of 22.5μl containing 1x Agilent fragmentation buffer and 2x Agilent blocking agent following the manufacturers' instructions. On completion of the fragmentation reaction, 22.5μl of 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to Agilent SurePrint G3 Mouse Gene Expression v2 8x60K Microarray(DesignID:074809) for 17 hours at 65°C in a rotating Agilent hybridization oven. After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent) and 1 minute with 37°C GE Wash buffer 2 (Agilent), then dried immediately by brief centrifugation.

scan_protocol: Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505C) using one color scan setting for 4x180k array slides (Scan Area 61x21.6 mm, Scan resolution 3um, Dye channel is set to Green and Green PMT is set to 100%)

data_processing: The scanned images were analyzed with Feature Extraction Software 10.7.1.1 (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities as the raw data. Raw data were normalized in quantile algorithm with Genespring 13.0(Agilent). Probe that at least 1 out of 2 samples flagged as Detected were maintained.

source_name: Activated hepatic stellate cells treated with sicav1-2

label_protocol: Cyanine-3 (Cy3) labeled cRNA was prepared from 0.2 μg RNA using the One-Color Low RNA Input Linear Amplification PLUS kit (Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA). Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.

growth_protocol: Primary hepatic stellate cells were prepared from 10 week old C57BL/6 mice via the two-step collagenase perfusion method.Then, isolated hepatic stellate cells were plated on 6-well plates and cultured in 1640 medium and 10% fetal bovine serum.

extract_protocol: RNA was extracted by mirVanaTM RNA Isolation Kit (Applied Biosystem p/n AM1556 ) following the manufacturer's instructions.

treatment_protocol: After attachment and culture 7days in hard plate, primary hepatic stellate cells were transfected overnight with 5 nM siRNA targeting Cav1 or a negative control siRNA using Lipo3000 transfection reagent according to the manufacturer’s instructions.

value: Activate hepatic stellate cells


title: si-cav3

source_name: Activated hepatic stellate cells treated with sicav1-3


title: KO_combo_Rep1


title: Y6M_3


title: Z. mobilis ZM4 cells, Control1, Min4 [C1]


title: nc2

source_name: Activate hepatic stellate cells-2

value: negative control


title: Y6M_1


title: nc3

source_name: Activate hepatic stellate cells-3


title: qhsc3

source_name: quiescent hepatic stelle cells-3

value: quiescent hepatic stelle cells


title: nc1

source_name: Activate hepatic stellate cells-1


title: si-cav1

source_name: Activated hepatic stellate cells treated with sicav1-1


title: Aged_NTN1_MSC_4

status: Public on Apr 14 2023

city: New York

first: David

last: Redmond

state: New York

address: 1300 York Avenue

institute: Weill Cornell Medicine

data_processing:
Reads were checked for quality (FastQC v0.11.5) and processed using the Digital Expression Explorer 2 (DEE2) workflow
Adapter trimming was performed with Skewer (v0.2.2)
Further quality control was performed with Minion, part of the Kraken package
Filtered reads were mapped to the mouse reference genome GRCm38 using STAR aligner and gene-wise expression counts were generated using the “-quantMode GeneCounts” parameter.
The R package edgeR was used to calculate FPKM
Assembly: mm10
Supplementary files format and content: tab-delimited text file include FPKM values for all samples in this subseries

source_name: Bone Marrow

extract_protocol:
Cells were FACS sorted into TRIzol-LS and total RNA was purified as per manufacturer’s protocol. 2 ng total RNA was utilized as input for library preparation.
Libraries for RNA-seq were prepared using SMART-Seq v4 Ultra Low Input RNA Kit following manufacturer's protocols.

treatment_protocol: Aged (18 month old) mice received 10 subcutaneous injections of PBS or recombinant NTN1 (4 µg) administered over a 2 week period. 72 hours following the last injection, mice were euthanized and their long bones harvested to isolate BM niche cells (MSCs and BMECs) by FACS for performing RNA Seq analyses.

value: Bone Marrow

value: Primary cells

value: Mesenchymal stromal cell

value: Aged (18 month old)

value: recombinant NTN1


title: Aged_NTN1_MSC_3


title: qhsc2

source_name: quiescent hepatic stelle cells-2


title: Aged_NTN1_MSC_1


title: Aged_PBS_MSC_4

value: PBS


title: qhsc1

source_name: quiescent hepatic stelle cells-1


title: Aged_PBS_MSC_1


title: Aged_PBS_BMEC_4

value: Endothelial cell


title: Aged_PBS_MSC_3


title: Aged_PBS_BMEC_3


title: Aged_PBS_BMEC_1


title: Aged_NTN1_HSC_3

description: RS-03684474_Aged-Female-NTN-HSC-3_RS-03677346_S30

treatment_protocol: Aged (18 month old) mice received 10 subcutaneous injections of PBS or recombinant NTN1 (4 µg) administered over a 2 week period. 72 hours following the last injection, mice were euthanized and their long bones harvested to isolate hematopoietic stem cells by FACS for performing RNA Seq analyses.

value: Hematopoietic stem cell


title: Aged_PBS_HSC_3

description: RS-03684471_Aged-Female-PBS-HSC-3_RS-03677343_S27


title: Aged_NTN1_HSC_2

description: RS-03684473_Aged-Female-NTN-HSC-2_RS-03677345_S29


title: Aged_NTN1_BMEC_3


title: Aged_PBS_HSC_2

description: RS-03684470_Aged-Female-PBS-HSC-2_RS-03677342_S26


title: Aged_PBS_MSC_2


title: Young_HSC_3

description: RS-03684447_Yng-Cntl-Female-HSC-3_RS-03677319_S3

value: Young (3 month old)


title: Aged_NTN1_BMEC_2


title: Aged_NTN1_BMEC_1


title: Aged_PBS_HSC_1

description: RS-03684469_Aged-Female-PBS-HSC-1_RS-03677341_S25


title: Aged_NTN1_MSC_2


title: Aged_BMEC_2

description: RS-03684458_Aged-Cntl-BMEC-2_RS-03677330_S14

treatment_protocol: To analyze aging associated transcriptional alterations within BMECs, RNA Seq analysis was performed on FACS purified BMECs isolated from young (3 month old) and aged (18 month old) mice.


title: Aged_NTN1_BMEC_4


title: genomic DNA from diabetic human limbal cells 19, passage 3

type: genomic

city: Los Angeles

first: Alexander

middle: V

last: Ljubimov

state: CA

address: 8700 Beverly Blvd.,

department: Biomedical Sciences

institute: Cedars-Sinai Medical Center

description: diabetic limbal sample

hyb_protocol: bisulphite converted DNA was amplified, fragmented and hybridized to Illumina InfiniumEPIC Human Methylation Beadchip v1.0 B4 using standard Illumina protocol

scan_protocol: Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting

data_processing: BeadStudio software v3.2

label: Cy5 and Cy3

source_name: limbal cells 19

label_protocol: Standard Illumina Protocol

growth_protocol: Cells were grown in EpiLife medium containing Human Keratinocyte Growth Supplement (HKGS), N-2 supplement, B27 supplement, 1X antibiotic/antimycotic mixture, with added 15 ng/ml epidermal growth factor (EGF)

extract_protocol: genomic DNA was extracted and purified from LBC samples using Qiagen DNeasy Kit according to standard instructions

treatment_protocol: untreated primary cultured cells

value: male

tag: sample type

value: corneolimbal cells

value: diabetic


title: Young_HSC_1

description: RS-03684445_Yng-Cntl-Female-HSC-1_RS-03677317_S1


title: Young_BMEC_3

description: RS-03684453_Yng-Cntl-BMEC-3_RS-03677325_S9


title: Aged_BMEC_3

description: RS-03684459_Aged-Cntl-BMEC-3_RS-03677331_S15


title: genomic DNA from diabetic human limbal cells 21, passage 3

description: diabetic limbal sample

source_name: limbal cells 21

tag: disease state


title: Young_HSC_2

description: RS-03684446_Yng-Cntl-Female-HSC-2_RS-03677318_S2

tag: genotype


title: genomic DNA from non-diabetic human limbal cells 14, passage 4

description: normal limbal sample

source_name: limbal cells 14

value: normal


title: Young_BMEC_2

description: RS-03684452_Yng-Cntl-BMEC-2_RS-03677324_S8

tag: treatment


title: Aged_BMEC_1

description: RS-03684457_Aged-Cntl-BMEC-1_RS-03677329_S13


title: Aged_MSC_3

description: RS-03534015_Aged-Lepr-3_RS-03519243_S6_R1_001

treatment_protocol: To analyze aging associated transcriptional alterations within BM MSCs, RNA Seq analysis was performed on FACS purified BM LepR+ MSCs isolated from young (3 month old) and aged (18 month old) mice.


title: Young_BMEC_1

description: RS-03684451_Yng-Cntl-BMEC-1_RS-03677323_S7


title: Young_MSC_3

description: RS-03534012_Yng-Lepr-3_RS-03519240_S3_R1_001


title: Aged_MSC_2

description: RS-03534014_Aged-Lepr-2_RS-03519242_S5_R1_001


title: Aged_MSC_1

description: RS-03534013_Aged-Lepr-1_RS-03519241_S4_R1_001


title: Young_MSC_2

description: RS-03534011_Yng-Lepr-2_RS-03519239_S2_R1_001


title: genomic DNA from non-diabetic human limbal cells 17, passage 2

description: normal limbal sample

source_name: limbal cells 17

value: female


title: genomic DNA from non-diabetic human limbal cells 15, passage 2

description: normal limbal sample

source_name: limbal cells 15


title: Young_MSC_1

description: RS-03534010_Yng-Lepr-1_RS-03519238_S1_R1_001


title: CDH5_NTN1KO_MSC_4

description: RS-03534054_V4-LEPR-RNA_RS-03519282_S63_R1_001

treatment_protocol: To perform RNA Seq analysis of BM niche cells following deletion of NTN1 specifically within LepR-derived MSCs, 5-6 month old LepRcre;Ntn1fl/fl mice (and cre negative littermate controls) were utilized.  To perform RNA Seq analysis of BM niche cells following deletion of NTN1 specifically within endothelial cells, CDH5-creERT2;Ntn1fl/fl mice were utilized. To induce Cdh5-creERT2-mediated recombination, mice were maintained on Custom Teklad 2020 Feed supplemented with 0.025% w/w tamoxifen (Envigo) ad libitum starting at 6-10 weeks of age for four consecutive weeks.  Age matched cre-negative littermate mice underwent the same tamoxifen induction regimen, and were utilized as controls. Mice were allowed to recover for 4 weeks post-tamoxifen induction prior to analysis. Mice were euthanized and their long bones harvested to isolate BM niche cells by FACS for performing RNA Seq analyses.

value: EC-specific NTN1 Knockout

value: Tamoxifen

value: young (4-6 month old)


title: genomic DNA from non-diabetic human limbal cells 13, passage 2

description: normal limbal sample

source_name: limbal cells 13


title: CDH5_NTN1KO_MSC_5

description: RS-03534055_V5-LEPR-RNA_RS-03519283_S64_R1_001

value: Bone Marrow


title: genomic DNA from non-diabetic human limbal cells 16, passage 2

description: normal limbal sample

source_name: limbal cells 16


title: Aged_NTN1_HSC_1

description: RS-03684472_Aged-Female-NTN-HSC-1_RS-03677344_S28

value: Primary cells


title: CDH5_NTN1KO_MSC_1

description: RS-03534051_V1-LEPR-RNA_RS-03519279_S60_R1_001

value: Mesenchymal stromal cell


title: CDH5_Control_MSC_5

description: RS-03534050_C5-LEPR-RNA_RS-03519278_S59_R1_001

value: Control


title: CDH5_Control_MSC_4

description: RS-03534049_C4-LEPR-RNA_RS-03519277_S58_R1_001


title: CDH5_NTN1KO_MSC_2

description: RS-03534052_V2-LEPR-RNA_RS-03519280_S61_R1_001


title: CDH5_Control_MSC_3

description: RS-03534048_C3-LEPR-RNA_RS-03519276_S57_R1_001


title: CDH5_NTN1KO_MSC_3

description: RS-03534053_V3-LEPR-RNA_RS-03519281_S62_R1_001


title: CDH5_Control_MSC_1

description: RS-03534046_C1-LEPR-RNA_RS-03519274_S55_R1_001


title: genomic DNA from diabetic human limbal cells 20, passage 3

description: diabetic limbal sample

source_name: limbal cells 20


title: genomic DNA from diabetic human limbal cells 22, passage 2

description: diabetic limbal sample

source_name: limbal cells 22


title: CDH5_NTN1KO_BMEC_5

description: RS-03534045_V5-EC-RNA_RS-03519273_S54_R1_001


title: CDH5_NTN1KO_BMEC_4

description: RS-03534044_V4-EC-RNA_RS-03519272_S53_R1_001


title: CDH5_NTN1KO_BMEC_3

description: RS-03534043_V3-EC-RNA_RS-03519271_S52_R1_001


title: genomic DNA from diabetic human limbal cells 18, passage 3

description: diabetic limbal sample

source_name: limbal cells 18


title: CDH5_NTN1KO_BMEC_2

description: RS-03534042_V2-EC-RNA_RS-03519270_S51_R1_001


title: CDH5_NTN1KO_BMEC_1

description: RS-03534041_V1-EC-RNA_RS-03519269_S50_R1_001


title: CDH5_Control_BMEC_4

description: RS-03534039_C4-EC-RNA_RS-03519267_S48_R1_001


title: CDH5_Control_BMEC_5

description: RS-03534040_C5-EC-RNA_RS-03519268_S49_R1_001


title: genomic DNA from non-diabetic human limbal cells 12, passage 6

description: normal limbal sample

source_name: limbal cells 12


title: CDH5_Control_BMEC_2

description: RS-03534037_C2-EC-RNA_RS-03519265_S46_R1_001


title: CDH5_Control_BMEC_1

description: RS-03534036_C1-EC-RNA_RS-03519264_S45_R1_001


title: LepR_NTN1KO_MSC_5

description: RS-03534035_Lepr-5-LeprPos-RNA_RS-03519263_S44_R1_001

value: MSC-specific NTN1 Knockout


title: LepR_NTN1KO_MSC_4

description: RS-03534034_Lepr-4-LeprPos-RNA_RS-03519262_S43_R1_001


title: LepR_NTN1KO_MSC_2

description: RS-03534032_Lepr-2-LeprPos-RNA_RS-03519260_S23_R1_001


title: LepR_NTN1KO_MSC_3

description: RS-03534033_Lepr-3-LeprPos-RNA_RS-03519261_S24_R1_001


title: LepR_NTN1KO_MSC_1

description: RS-03534031_Lepr-1-LeprPos-RNA_RS-03519259_S22_R1_001


title: LepR_Control_MSC_5

description: RS-03534030_Cntl-5-LeprPos-RNA_RS-03519258_S21_R1_001


title: Aged_PBS_BMEC_2

value: Aged (18 month old)

value: PBS


title: LepR_Control_MSC_1

description: RS-03534026_Cntl-1-LeprPos-RNA_RS-03519254_S17_R1_001


title: LepR_NTN1KO_BMEC_5

description: RS-03534025_Lepr-5-BM-EC-RNA_RS-03519253_S16_R1_001

value: Endothelial cell


title: LepR_NTN1KO_BMEC_4

description: RS-03534024_Lepr-4-BM-EC-RNA_RS-03519252_S15_R1_001


title: LepR_Control_MSC_3

description: RS-03534028_Cntl-3-LeprPos-RNA_RS-03519256_S19_R1_001


title: LepR_NTN1KO_BMEC_3

description: RS-03534023_Lepr-3-BM-EC-RNA_RS-03519251_S14_R1_001


title: LepR_Control_BMEC_5

description: RS-03534020_Cntl-5-BM-EC-RNA_RS-03519248_S11_R1_001


title: LepR_Control_BMEC_4

description: RS-03534019_Cntl-4-BM-EC-RNA_RS-03519247_S10_R1_001


title: LepR_NTN1KO_BMEC_2

description: RS-03534022_Lepr-2-BM-EC-RNA_RS-03519250_S13_R1_001


title: LepR_NTN1KO_BMEC_1

description: RS-03534021_Lepr-1-BM-EC-RNA_RS-03519249_S12_R1_001


title: LepR_Control_BMEC_3

description: RS-03534018_Cntl-3-BM-EC-RNA_RS-03519246_S9_R1_001


title: LepR_Control_BMEC_1

description: RS-03534016_Cntl-1-BM-EC-RNA_RS-03519244_S7_R1_001


title: LepR_Control_BMEC_2

description: RS-03534017_Cntl-2-BM-EC-RNA_RS-03519245_S8_R1_001


title: 10C_M_999

city: La Jolla

first: Joseph

middle: R

last: Ecker

address: 10010 North Torrey Pines Road

department: Genomic Analysis Laboratory

institute: HHMI-Salk-Institute

data_processing:
Reads trimed with Cutadapt for adapters and low quality bases (Phred score >= 20)
The resulting files are mapped to in-silico bisulfite converted mm10 reference genome using Bismark.
The raw BAM file will be filtered using samtools to filter reads by MAPQ > 10 and use Picard to remove PCR duplicates
Tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls will be generated with in-house developing package using the filtered BAM files
Assembly: mm10
Supplementary files format and content: tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls. Columns are: 1. Chromosome, 2. Position, 3. Strand, 4. Cytosin Context, 5. methylated base call, 6. total mapped base call, 7. not used, place holder column
Library strategy: snmC-seq2

source_name: C57BL/6J Male P56 Mouse Brain

growth_protocol: All mice are C57BL/6J Male, brain dissected in P56

extract_protocol:
the brain of P56 male C57BL/6J mouse will be removed and cut into 600 microns thick coronal sections using a tissue matrix. Then each region will be microdissected from the coronal tissue sections based on identified landmarks and brain atlas coordinates, as defined in Allen brain atlas CCF3 (Avaliable at: http://atlas.brain-map.org). For each dissection region, the tissue from several littermate mice will be combined together into to separate replicate groups. The number of animals in a replicate depends on the size of the tissue. Nuclei preparation will be stained with the fluorescently labeled anti-NeuN body and nucleic acid stain Hoechst 33342.
Library prepared following protocol discribed in Luo, Chongyuan, Angeline Rivkin, Jingtian Zhou, Justin P. Sandoval, Laurie Kurihara, Jacinta Lucero, Rosa Castanon, et al. 2018. “Robust Single-Cell DNA Methylome Profiling with snmC-seq2.” Nature Communications 9 (1): 3824.

tag: brain region

tag: Sex


title: 10C_M_997


title: 10C_M_996


title: 10C_M_998


title: CDH5_Control_MSC_2

description: RS-03534047_C2-LEPR-RNA_RS-03519275_S56_R1_001

tag: cell line


title: 10C_M_992

tag: strain


title: 10C_M_993


title: 10C_M_994


title: 10C_M_991


title: LepR_Control_MSC_4

description: RS-03534029_Cntl-4-LeprPos-RNA_RS-03519257_S20_R1_001


title: 10C_M_990


title: 10C_M_988


title: 10C_M_984


title: 10C_M_986


title: 10C_M_981


title: 10C_M_983


title: 10C_M_976


title: 10C_M_977


title: 10C_M_987


title: CDH5_Control_BMEC_3

description: RS-03534038_C3-EC-RNA_RS-03519266_S47_R1_001


title: LepR_Control_MSC_2

description: RS-03534027_Cntl-2-LeprPos-RNA_RS-03519255_S18_R1_001


title: 10C_M_98


title: 10C_M_978


title: 10C_M_995


title: 10C_M_974


title: 10C_M_975


title: 10C_M_972


title: 10C_M_980


title: 10C_M_982


title: 10C_M_970


title: 10C_M_97


title: 10C_M_973


title: 10C_M_968


title: 10C_M_965


title: 10C_M_969


title: 10C_M_989


title: 10C_M_963


title: 10C_M_966


title: 10C_M_961


title: 10C_M_960


title: 10C_M_959


title: 10C_M_954


title: 10C_M_957


title: 10C_M_956


title: 10C_M_971


title: 10C_M_952


title: 10C_M_950


title: 10C_M_951


title: 10C_M_947


title: 10C_M_967


title: 10C_M_946


title: 10C_M_945


title: 10C_M_949


title: 10C_M_948


title: 10C_M_964


title: 10C_M_95


title: 10C_M_942


title: 10C_M_941


title: 10C_M_944


title: 10C_M_940


title: 10C_M_939


title: 10C_M_94


title: 10C_M_962


title: 10C_M_938


title: 10C_M_958


title: 10C_M_937


title: 10C_M_936


title: 10C_M_953


title: 10C_M_955


title: 10C_M_935


title: 10C_M_933


title: 10C_M_931


title: 10C_M_930


title: 10C_M_932


title: 10C_M_929


title: 10C_M_928


title: 10C_M_925


title: 10C_M_922


title: 10C_M_927


title: 10C_M_924


title: 10C_M_921


title: 10C_M_926


title: 10C_M_919


title: 10C_M_920


title: 10C_M_923


title: 10C_M_918


title: 10C_M_915


title: 10C_M_916


title: 10C_M_917


title: 10C_M_914


title: 10C_M_912


title: 10C_M_910


title: 10C_M_913


title: 10C_M_91


title: 10C_M_934


title: 10C_M_93


title: 10C_M_909


title: 10C_M_905


title: 10C_M_907


title: 10C_M_904


title: 10C_M_902


title: 10C_M_903


title: 10C_M_901


title: 10C_M_900


title: 10C_M_90


title: 10C_M_9


title: 10C_M_899


title: 10C_M_895


title: 10C_M_896


title: 10C_M_92


title: 10C_M_890


title: 10C_M_888


title: 10C_M_889


title: 10C_M_887


title: 10C_M_893


title: 10C_M_886


title: 10C_M_911


title: 10C_M_885


title: 10C_M_883


title: 10C_M_884


title: 10C_M_882


title: 10C_M_881


title: 10C_M_880


title: 10C_M_88


title: 10C_M_877


title: 10C_M_879


title: 10C_M_875


title: 10C_M_876


title: 10C_M_873


title: 10C_M_897


title: 10C_M_894


title: 10C_M_891


title: 10C_M_87


title: 10C_M_871


title: 10C_M_868


title: 10C_M_870


title: 10C_M_869


title: 10C_M_867


title: 10C_M_863


title: 10C_M_864


title: 10C_M_866


title: 10C_M_862


title: 10C_M_861


title: 10C_M_865


title: 10C_M_860


title: 10C_M_86


title: 10C_M_859


title: 10C_M_857


title: 10C_M_858


title: 10C_M_856


title: 10C_M_878


title: 10C_M_855


title: 10C_M_852


title: 10C_M_872


title: 10C_M_851


title: 10C_M_850


title: 10C_M_85


title: 10C_M_853


title: 10C_M_849


title: 10C_M_848


title: 10C_M_846


title: 10C_M_847


title: 10C_M_844


title: 10C_M_845


title: 10C_M_843


title: 10C_M_841


title: 10C_M_835


title: 10C_M_840


title: 10C_M_838


title: 10C_M_834


title: 10C_M_833


title: 10C_M_836


title: 10C_M_839


title: 10C_M_837


title: 10C_M_829


title: 10C_M_828


title: 10C_M_831


title: 10C_M_827


title: 10C_M_823


title: 10C_M_825


title: 10C_M_824


title: 10C_M_820


title: 10C_M_82


title: 10C_M_821


title: 10C_M_819


title: 10C_M_842


title: 10C_M_84


title: 10C_M_818


title: 10C_M_832


title: 10C_M_816


title: 10C_M_817


title: 10C_M_814


title: 10C_M_813


title: 10C_M_83


title: 10C_M_811


title: 10C_M_81


title: 10C_M_809


title: 10C_M_807


title: 10C_M_826


title: 10C_M_806


title: 10C_M_801


title: 10C_M_803


title: 10C_M_802


title: 10C_M_804


title: 10C_M_800


title: 10C_M_8


title: 10C_M_80


title: 10C_M_815


title: 10C_M_797


title: 10C_M_798


title: 10C_M_799


title: 10C_M_796


title: 10C_M_795


title: 10C_M_794


title: 10C_M_812


title: 10C_M_793


title: 10C_M_810


title: 10C_M_789


title: 10C_M_791


title: 10C_M_790


title: 10C_M_788


title: 10C_M_787


title: 10C_M_805


title: 10C_M_79


title: 10C_M_784


title: 10C_M_785


title: 10C_M_783


title: 10C_M_782


title: 10C_M_786


title: 10C_M_78


title: 10C_M_779


title: 10C_M_778


title: 10C_M_775


title: 10C_M_777


title: 10C_M_774


title: 10C_M_776


title: 10C_M_772


title: 10C_M_771


title: 10C_M_773


title: 10C_M_77


title: 10C_M_766


title: 10C_M_767


title: 10C_M_768


title: 10C_M_764


title: 10C_M_76


title: 10C_M_762


title: 10C_M_759


title: 10C_M_781


title: 10C_M_760


title: 10C_M_770


title: 10C_M_756


title: 10C_M_758


title: 10C_M_757


title: 10C_M_753


title: 10C_M_754


title: 10C_M_755


title: 10C_M_752


title: 10C_M_751


title: 10C_M_765


title: 10C_M_748


title: 10C_M_750


title: 10C_M_75


title: 10C_M_747


title: 10C_M_749


title: 10C_M_744


title: 10C_M_745


title: 10C_M_743


title: 10C_M_742


title: 10C_M_741


title: 10C_M_761


title: 10C_M_74


title: 10C_M_740


title: 10C_M_739


title: 10C_M_734


title: 10C_M_737


title: 10C_M_735


title: 10C_M_733


title: 10C_M_732


title: 10C_M_731


title: 10C_M_730


title: 10C_M_736


title: 10C_M_738


title: 10C_M_73


title: 10C_M_729


title: 10C_M_727


title: 10C_M_728


title: 10C_M_746


title: 10C_M_720


title: 10C_M_72


title: 10C_M_723


title: 10C_M_726


title: 10C_M_725


title: 10C_M_724


title: 10C_M_719


title: 10C_M_711


title: 10C_M_714


title: 10C_M_713


title: 10C_M_716


title: 10C_M_717


title: 10C_M_710


title: 10C_M_715


title: 10C_M_707


title: 10C_M_705


title: 10C_M_708


title: 10C_M_71


title: 10C_M_706


title: 10C_M_704


title: 10C_M_702


title: 10C_M_70


title: 10C_M_7


title: 10C_M_703


title: 10C_M_709


title: 10C_M_699


title: 10C_M_701


title: 10C_M_700


title: 10C_M_698


title: 10C_M_696


title: 10C_M_694


title: 10C_M_692


title: 10C_M_693


title: 10C_M_697


title: 10C_M_691


title: 10C_M_69


title: 10C_M_690


title: 10C_M_688


title: 10C_M_689


title: 10C_M_685


title: 10C_M_686


title: 10C_M_683


title: 10C_M_679


title: 10C_M_681


title: 10C_M_682


title: 10C_M_684


title: 10C_M_677


title: 10C_M_675


title: 10C_M_676


title: 10C_M_678


title: 10C_M_695


title: 10C_M_680


title: 10C_M_68


title: 10C_M_671


title: 10C_M_674


title: 10C_M_672


title: 10C_M_673


title: 10C_M_670


title: 10C_M_668


title: 10C_M_669


title: 10C_M_687


title: 10C_M_67


title: 10C_M_664


title: 10C_M_666


title: 10C_M_662


title: 10C_M_661


title: 10C_M_663


title: 10C_M_660


title: 10C_M_665


title: 10C_M_66


title: 10C_M_658


title: 10C_M_657


title: 10C_M_659


title: 10C_M_654


title: 10C_M_655


title: 10C_M_653


title: 10C_M_651


title: 10C_M_650


title: 10C_M_649


title: 10C_M_648


title: 10C_M_646


title: 10C_M_667


title: 10C_M_647


title: 10C_M_645


title: 10C_M_642


title: 10C_M_644


title: 10C_M_641


title: 10C_M_640


title: 10C_M_64


title: 10C_M_636


title: 10C_M_638


title: 10C_M_656


title: 10C_M_635


title: 10C_M_634


title: 10C_M_637


title: 10C_M_633


title: 10C_M_632


title: 10C_M_639


title: 10C_M_63


title: 10C_M_65


title: 10C_M_631


title: 10C_M_652


title: 10C_M_629


title: 10C_M_628


title: 10C_M_627


title: 10C_M_626


title: 10C_M_624


title: 10C_M_643


title: 10C_M_623


title: 10C_M_622


title: 10C_M_62


title: 10C_M_620


title: 10C_M_619


title: 10C_M_618


title: 10C_M_617


title: 10C_M_614


title: 10C_M_616


title: 10C_M_611


title: 10C_M_612


title: 10C_M_613


title: 10C_M_630


title: 10C_M_615


title: 10C_M_610


title: 10C_M_608


title: 10C_M_61


title: 10C_M_606


title: 10C_M_607


title: 10C_M_609


title: 10C_M_603


title: 10C_M_625


title: 10C_M_601


title: 10C_M_605


title: 10C_M_60


title: 10C_M_621


title: 10C_M_599


title: 10C_M_598


title: 10C_M_600


title: 10C_M_595


title: 10C_M_596


title: 10C_M_597


title: 10C_M_593


title: 10C_M_594


title: 10C_M_591


title: 10C_M_592


title: 10C_M_588


title: 10C_M_586


title: 10C_M_587


title: 10C_M_602


title: 10C_M_583


title: 10C_M_585


title: 10C_M_580


title: 10C_M_582


title: 10C_M_763


title: 10C_M_604


title: 10C_M_579


title: 10C_M_581


title: 10C_M_573


title: 10C_M_578


title: 10C_M_570


title: 10C_M_572


title: 10C_M_576


title: 10C_M_575


title: 10C_M_58


title: 10C_M_571


title: 10C_M_569


title: 10C_M_589


title: 10C_M_590


title: 10C_M_584


title: 10C_M_565


title: 10C_M_564


title: 10C_M_563


title: 10C_M_567


title: 10C_M_562


title: 10C_M_566


title: 10C_M_556


title: 10C_M_559


title: 10C_M_557


title: 10C_M_56


title: 10C_M_560


title: 10C_M_554


title: 10C_M_561


title: 10C_M_555


title: 10C_M_552


title: 10C_M_553


title: 10C_M_55


title: 10C_M_550


title: 10C_M_568


title: 10C_M_57


title: 10C_M_551


title: 10C_M_547


title: 10C_M_548


title: 10C_M_549


title: 10C_M_545


title: 10C_M_546


title: 10C_M_543


title: 10C_M_542


title: 10C_M_540


title: 10C_M_539


title: 10C_M_537


title: 10C_M_536


title: 10C_M_534


title: 10C_M_530


title: 10C_M_531


title: 10C_M_533


title: 10C_M_529


title: 10C_M_527


title: 10C_M_53


title: 10C_M_526


title: 10C_M_525


title: 10C_M_521


title: 10C_M_524


title: 10C_M_520


title: 10C_M_517


title: 10C_M_52


title: 10C_M_541


title: 10C_M_514


title: 10C_M_515


title: 10C_M_54


title: 10C_M_522


title: 10C_M_518


title: 10C_M_538


title: 10C_M_519


title: 10C_M_513


title: 10C_M_512


title: 10C_M_511


title: 10C_M_532


title: 10C_M_523


title: 10C_M_505


title: 10C_M_506


title: 10C_M_504


title: 10C_M_507


title: 10C_M_503


title: 10C_M_501


title: 10C_M_499


title: 10C_M_516


title: 10C_M_50


title: 10C_M_5


title: 10C_M_500


title: 10C_M_498


title: 10C_M_502


title: 10C_M_496


title: 10C_M_497


title: 10C_M_495


title: 10C_M_494


title: 10C_M_492


title: 10C_M_493


title: 10C_M_491


title: 10C_M_510


title: 10C_M_51


title: 10C_M_490


title: 10C_M_508


title: 10C_M_489


title: 10C_M_49


title: 10C_M_488


title: 10C_M_487


title: 10C_M_509


title: 10C_M_486


title: 10C_M_485


title: 10C_M_483


title: 10C_M_482


title: 10C_M_48


title: 10C_M_480


title: 10C_M_479


title: 10C_M_474


title: 10C_M_472


title: 10C_M_471


title: 10C_M_476


title: 10C_M_469


title: 10C_M_475


title: 10C_M_468


title: 10C_M_47


title: 10C_M_466


title: 10C_M_478


title: 10C_M_463


title: 10C_M_477


title: 10C_M_470


title: 10C_M_465


title: 10C_M_461


title: 10C_M_460


title: 10C_M_462


title: 10C_M_484


title: 10C_M_467


title: 10C_M_457


title: 10C_M_456


title: 10C_M_46


title: 10C_M_458


title: 10C_M_459


title: 10C_M_455


title: 10C_M_451


title: 10C_M_473


title: 10C_M_452


title: 10C_M_450


title: 10C_M_445


title: 10C_M_448


title: 10C_M_443


title: 10C_M_442


title: 10C_M_438


title: 10C_M_446


title: 10C_M_437


title: 10C_M_449


title: 10C_M_44


title: 10C_M_447


title: 10C_M_435


title: 10C_M_454


title: 10C_M_45


title: 10C_M_439


title: 10C_M_433


title: 10C_M_441


title: 10C_M_440


title: 10C_M_431


title: 10C_M_432


title: 10C_M_434


title: 10C_M_429


title: 10C_M_430


title: 10C_M_43


title: 10C_M_428


title: 10C_M_425


title: 10C_M_444


title: 10C_M_423


title: 10C_M_422


title: 10C_M_426


title: 10C_M_424


title: 10C_M_421


title: 10C_M_42


title: 10C_M_414


title: 10C_M_409


title: 10C_M_418


title: 10C_M_41


title: 10C_M_411


title: 10C_M_413


title: 10C_M_406


title: 10C_M_408


title: 10C_M_415


title: 10C_M_419


title: 10C_M_412


title: 10C_M_407


title: 10C_M_417


title: 10C_M_410


title: 10C_M_405


title: 10C_M_404


title: 10C_M_402


title: 10C_M_403


title: 10C_M_400


title: 10C_M_40


title: 10C_M_401


title: 10C_M_4


title: 10C_M_399


title: 10C_M_398


title: 10C_M_394


title: 10C_M_395


title: 10C_M_391


title: 10C_M_397


title: 10C_M_392


title: 10C_M_390


title: 10C_M_39


title: 10C_M_388


title: 10C_M_389


title: 10C_M_380


title: 10C_M_381


title: 10C_M_382


title: 10C_M_385


title: 10C_M_38


title: 10C_M_378


title: 10C_M_377


title: 10C_M_375


title: 10C_M_376


title: 10C_M_396


title: 10C_M_373


title: 10C_M_374


title: 10C_M_371


title: 10C_M_370


title: 10C_M_387


title: 10C_M_37


title: 10C_M_369


title: 10C_M_379


title: 10C_M_366


title: 10C_M_365


title: 10C_M_364


title: 10C_M_383


title: 10C_M_372


title: 10C_M_362


title: 10C_M_361


title: 10C_M_360


title: 10C_M_36


title: 10C_M_356


title: 10C_M_367


title: 10C_M_353


title: 10C_M_352


title: 10C_M_357


title: 10C_M_386


title: 10C_M_351


title: 10C_M_350


title: 10C_M_346


title: 10C_M_359


title: 10C_M_348


title: 10C_M_345


title: 10C_M_344


title: 10C_M_349


title: 10C_M_363


title: 10C_M_342


title: 10C_M_347


title: 10C_M_343


title: 10C_M_341


title: 10C_M_34


title: 10C_M_332


title: 10C_M_336


title: 10C_M_335


title: 10C_M_333


title: 10C_M_35


title: 10C_M_330


title: 10C_M_331


title: 10C_M_33


title: 10C_M_323


title: 10C_M_327


title: 10C_M_326


title: 10C_M_325


title: 10C_M_322


title: 10C_M_368


title: 10C_M_320


title: 10C_M_318


title: 10C_M_317


title: 10C_M_316


title: 10C_M_315


title: 10C_M_319


title: 10C_M_32


title: 10C_M_314


title: 10C_M_340


title: 10C_M_337


title: 10C_M_321


title: 10C_M_31


title: 10C_M_312


title: 10C_M_308


title: 10C_M_329


title: 10C_M_309


title: 10C_M_328


title: 10C_M_306


title: 10C_M_305


title: 10C_M_303


title: 10C_M_302


title: 10C_M_339


title: 10C_M_301


title: 10C_M_304


title: 10C_M_300


title: 10C_M_297


title: 10C_M_30


title: 10C_M_296


title: 10C_M_299


title: 10C_M_298


title: 10C_M_3


title: 10C_M_293


title: 10C_M_311


title: 10C_M_291


title: 10C_M_290


title: 10C_M_292


title: 10C_M_307


title: 10C_M_310


title: 10C_M_29


title: 10C_M_286


title: 10C_M_287


title: 10C_M_285


title: 10C_M_284


title: 10C_M_283


title: 10C_M_289


title: 10C_M_281


title: 10C_M_280


title: 10C_M_28


title: 10C_M_278


title: 10C_M_277


title: 10C_M_295


title: 10C_M_279


title: 10C_M_294


title: 10C_M_276


title: 10C_M_275


title: 10C_M_271


title: 10C_M_272


title: 10C_M_268


title: 10C_M_274


title: 10C_M_269


title: 10C_M_27


title: 10C_M_265


title: 10C_M_264


title: 10C_M_262


title: 10C_M_263


title: 10C_M_260


title: 10C_M_261


title: 10C_M_26


title: 10C_M_267


title: 10C_M_282


title: 10C_M_257


title: 10C_M_256


title: 10C_M_259


title: 10C_M_254


title: 10C_M_255


title: 10C_M_252


title: 10C_M_253


title: 10C_M_250


title: 10C_M_251


title: 10C_M_25


title: 10C_M_270


title: 10C_M_249


title: 10C_M_273


title: 10C_M_243


title: 10C_M_247


title: 10C_M_244


title: 10C_M_246


title: 10C_M_242


title: 10C_M_240


title: 10C_M_241


title: 10C_M_258


title: 10C_M_245


title: 10C_M_24


title: 10C_M_239


title: 10C_M_238


title: 10C_M_237


title: 10C_M_234


title: 10C_M_236


title: 10C_M_233


title: 10C_M_228


title: 10C_M_231


title: 10C_M_229


title: 10C_M_230


title: 10C_M_248


title: 10C_M_224


title: 10C_M_226


title: 10C_M_223


title: 10C_M_22


title: 10C_M_221


title: 10C_M_217


title: 10C_M_219


title: 10C_M_214


title: 10C_M_216


title: 10C_M_212


title: 10C_M_215


title: 10C_M_220


title: 10C_M_209


title: 10C_M_21


title: 10C_M_211


title: 10C_M_23


title: 10C_M_207


title: 10C_M_208


title: 10C_M_227


title: 10C_M_206


title: 10C_M_225


title: 10C_M_203


title: 10C_M_205


title: 10C_M_201


title: 10C_M_20


title: 10C_M_218


title: 10C_M_2


title: 10C_M_196


title: 10C_M_197


title: 10C_M_198


title: 10C_M_194


title: 10C_M_210


title: 10C_M_195


title: 10C_M_192


title: 10C_M_193


title: 10C_M_190


title: 10C_M_222


title: 10C_M_191


title: 10C_M_188


title: 10C_M_204


title: 10C_M_187


title: 10C_M_189


title: 10C_M_185


title: 10C_M_19


title: 10C_M_200


title: 10C_M_186


title: 10C_M_182


title: 10C_M_183


title: 10C_M_202


title: 10C_M_181


title: 10C_M_18


title: 10C_M_179


title: 10C_M_178


title: 10C_M_199


title: 10C_M_177


title: 10C_M_355


title: 10C_M_180


title: 10C_M_176


title: 10C_M_174


title: 10C_M_175


title: 10C_M_173


title: 10C_M_171


title: 10C_M_172


title: 10C_M_170


title: 10C_M_17


title: 10C_M_168


title: 10C_M_169


title: 10C_M_167


title: 10C_M_166


title: 10C_M_164


title: 10C_M_161


title: 10C_M_160


title: 10C_M_155


title: 10C_M_158


title: 10C_M_157


title: 10C_M_162


title: 10C_M_165


title: 10C_M_154


title: 10C_M_156


title: 10C_M_152


title: 10C_M_153


title: 10C_M_1518


title: 10C_M_1517


title: 10C_M_1519


title: 10C_M_1516


title: 10C_M_16


title: 10C_M_1515


title: 10C_M_1514


title: 10C_M_1511


title: 10C_M_1513


title: 10C_M_1512


title: 10C_M_1510


title: 10C_M_163


title: 10C_M_151


title: 10C_M_1504


title: 10C_M_1507


title: 10C_M_1508


title: 10C_M_1505


title: 10C_M_1503


title: 10C_M_1500


title: 10C_M_1499


title: 10C_M_150


title: 10C_M_1506


title: 10C_M_15


title: 10C_M_1498


title: 10C_M_1497


title: 10C_M_1501


title: 10C_M_1496


title: 10C_M_1495


title: 10C_M_1493


title: 10C_M_1494


title: 10C_M_1492


title: 10C_M_1491


title: 10C_M_1490


title: 10C_M_1509


title: 10C_M_149


title: 10C_M_1489


title: 10C_M_1488


title: 10C_M_1485


title: 10C_M_1484


title: 10C_M_1486


title: 10C_M_1483


title: 10C_M_1482


title: 10C_M_1481


title: 10C_M_1480


title: 10C_M_1477


title: 10C_M_1478


title: 10C_M_1479


title: 10C_M_1476


title: 10C_M_148


title: 10C_M_1470


title: 10C_M_1471


title: 10C_M_1475


title: 10C_M_1469


title: 10C_M_1468


title: 10C_M_1473


title: 10C_M_1466


title: 10C_M_1472


title: 10C_M_1462


title: 10C_M_1474


title: 10C_M_1465


title: 10C_M_1463


title: 10C_M_1460


title: 10C_M_1467


title: 10C_M_1464


title: 10C_M_1461


title: 10C_M_1457


title: 10C_M_1459


title: 10C_M_1455


title: 10C_M_1458


title: 10C_M_1456


title: 10C_M_1453


title: 10C_M_1454


title: 10C_M_1451


title: 10C_M_1452


title: 10C_M_1450


title: 10C_M_145


title: 10C_M_1448


title: 10C_M_1447


title: 10C_M_1446


title: 10C_M_1440


title: 10C_M_144


title: 10C_M_1441


title: 10C_M_1439


title: 10C_M_1438


title: 10C_M_1436


title: 10C_M_1435


title: 10C_M_1434


title: 10C_M_1433


title: 10C_M_1443


title: 10C_M_1429


title: 10C_M_1432


title: 10C_M_1431


title: 10C_M_1430


title: 10C_M_1428


title: 10C_M_1426


title: 10C_M_1425


title: 10C_M_1424


title: 10C_M_1420


title: 10C_M_1421


title: 10C_M_1423


title: 10C_M_1419


title: 10C_M_1449


title: 10C_M_1422


title: 10C_M_1437


title: 10C_M_1417


title: 10C_M_1414


title: 10C_M_1413


title: 10C_M_1416


title: 10C_M_1415


title: 10C_M_1412


title: 10C_M_1411


title: 10C_M_141


title: 10C_M_1410


title: 10C_M_1409


title: 10C_M_1406


title: 10C_M_1407


title: 10C_M_1405


title: 10C_M_1403


title: 10C_M_1404


title: 10C_M_1400


title: 10C_M_143


title: 10C_M_140


title: 10C_M_142


title: 10C_M_1402


title: 10C_M_1418


title: 10C_M_1394


title: 10C_M_1395


title: 10C_M_1397


title: 10C_M_1399


title: 10C_M_1396


title: 10C_M_1393


title: 10C_M_1390


title: 10C_M_1389


title: 10C_M_1391


title: 10C_M_139


title: 10C_M_1387


title: 10C_M_1386


title: 10C_M_1385


title: 10C_M_1383


title: 10C_M_1388


title: 10C_M_1382


title: 10C_M_1384


title: 10C_M_1401


title: 10C_M_1379


title: 10C_M_138


title: 10C_M_14


title: 10C_M_1398


title: 10C_M_1380


title: 10C_M_1378


title: 10C_M_1376


title: 10C_M_1377


title: 10C_M_1392


title: 10C_M_1374


title: 10C_M_1375


title: 10C_M_1373


title: 10C_M_1371


title: 10C_M_137


title: 10C_M_1372


title: 10C_M_1369


title: 10C_M_1368


title: 10C_M_1366


title: 10C_M_1367


title: 10C_M_1364


title: 10C_M_1363


title: 10C_M_1365


title: 10C_M_1381


title: 10C_M_136


title: 10C_M_1361


title: 10C_M_1370


title: 10C_M_1355


title: 10C_M_1358


title: 10C_M_1357


title: 10C_M_1356


title: 10C_M_1349


title: 10C_M_1348


title: 10C_M_135


title: 10C_M_1353


title: 10C_M_1350


title: 10C_M_1352


title: 10C_M_1344


title: 10C_M_1347


title: 10C_M_1342


title: 10C_M_1345


title: 10C_M_1343


title: 10C_M_1362


title: 10C_M_1341


title: 10C_M_1360


title: 10C_M_134


title: 10C_M_1340


title: 10C_M_1339


title: 10C_M_1359


title: 10C_M_1333


title: 10C_M_1336


title: 10C_M_1337


title: 10C_M_1334


title: 10C_M_1335


title: 10C_M_133


title: 10C_M_1346


title: 10C_M_1325


title: 10C_M_1331


title: 10C_M_1321


title: 10C_M_1324


title: 10C_M_1319


title: 10C_M_1323


title: 10C_M_1317


title: 10C_M_1322


title: 10C_M_132


title: 10C_M_1316


title: 10C_M_1313


title: 10C_M_1315


title: 10C_M_1314


title: 10C_M_1312


title: 10C_M_1318


title: 10C_M_1330


title: 10C_M_1338


title: 10C_M_1310


title: 10C_M_1311


title: 10C_M_131


title: 10C_M_1307


title: 10C_M_1306


title: 10C_M_1320


title: 10C_M_1329


title: 10C_M_1327


title: 10C_M_1308


title: 10C_M_1326


title: 10C_M_1304


title: 10C_M_1305


title: 10C_M_1302


title: 10C_M_1301


title: 10C_M_1300


title: 10C_M_130


title: 10C_M_13


title: 10C_M_1298


title: 10C_M_1297


title: 10C_M_1296


title: 10C_M_1289


title: 10C_M_1288


title: 10C_M_1294


title: 10C_M_1295


title: 10C_M_129


title: 10C_M_1293


title: 10C_M_1286


title: 10C_M_1283


title: 10C_M_1284


title: 10C_M_1282


title: 10C_M_1281


title: 10C_M_1287


title: 10C_M_1280


title: 10C_M_1279


title: 10C_M_1277


title: 10C_M_1278


title: 10C_M_1299


title: 10C_M_1276


title: 10C_M_1273


title: 10C_M_1274


title: 10C_M_1291


title: 10C_M_1272


title: 10C_M_1270


title: 10C_M_1271


title: 10C_M_1303


title: 10C_M_1309


title: 10C_M_128


title: 10C_M_1267


title: 10C_M_1268


title: 10C_M_1285


title: 10C_M_126


title: 10C_M_1262


title: 10C_M_127


title: 10C_M_1259


title: 10C_M_1264


title: 10C_M_1260


title: 10C_M_1269


title: 10C_M_1258


title: 10C_M_1255


title: 10C_M_1257


title: 10C_M_1256


title: 10C_M_1275


title: 10C_M_1254


title: 10C_M_1253


title: 10C_M_1252


title: 10C_M_1248


title: 10C_M_125


title: 10C_M_1250


title: 10C_M_1251


title: 10C_M_1247


title: 10C_M_1249


title: 10C_M_1246


title: 10C_M_1265


title: 10C_M_1266


title: 10C_M_1261


title: 10C_M_1244


title: 10C_M_1241


title: 10C_M_1263


title: 10C_M_1243


title: 10C_M_1240


title: 10C_M_124


title: 10C_M_1245


title: 10C_M_1237


title: 10C_M_1238


title: 10C_M_1235


title: 10C_M_1239


title: 10C_M_1233


title: 10C_M_1234


title: 10C_M_1231


title: 10C_M_1230


title: 10C_M_123


title: 10C_M_1232


title: 10C_M_1226


title: 10C_M_1227


title: 10C_M_1224


title: 10C_M_1228


title: 10C_M_1225


title: 10C_M_1222


title: 10C_M_1221


title: 10C_M_122


title: 10C_M_1218


title: 10C_M_1223


title: 10C_M_1220


title: 10C_M_1219


title: 10C_M_1242


title: 10C_M_1216


title: 10C_M_1213


title: 10C_M_1215


title: 10C_M_1217


title: 10C_M_1212


title: 10C_M_1211


title: 10C_M_1209


title: 10C_M_1208


title: 10C_M_1207


title: 10C_M_1205


title: 10C_M_1236


title: 10C_M_1202


title: 10C_M_1206


title: 10C_M_120


title: 10C_M_12


title: 10C_M_1199


title: 10C_M_1200


title: 10C_M_1201


title: 10C_M_1204


title: 10C_M_1195


title: 10C_M_1198


title: 10C_M_1197


title: 10C_M_1193


title: 10C_M_1192


title: 10C_M_1194


title: 10C_M_1191


title: 10C_M_1196


title: 10C_M_1190


title: 10C_M_121


title: 10C_M_119


title: 10C_M_1188


title: 10C_M_1185


title: 10C_M_1187


title: 10C_M_1184


title: 10C_M_1189


title: 10C_M_1178


title: 10C_M_1181


title: 10C_M_1182


title: 10C_M_1180


title: 10C_M_1183


title: 10C_M_118


title: 10C_M_1179


title: 10C_M_1186


title: 10C_M_1177


title: 10C_M_1175


title: 10C_M_1173


title: 10C_M_1172


title: 10C_M_117


title: 10C_M_1169


title: 10C_M_1165


title: 10C_M_1163


title: 10C_M_1168


title: 10C_M_1166


title: 10C_M_1167


title: 10C_M_1162


title: 10C_M_1160


title: 10C_M_1171


title: 10C_M_1158


title: 10C_M_1161


title: 10C_M_1159


title: 10C_M_1157


title: 10C_M_1164


title: 10C_M_1156


title: 10C_M_116


title: 10C_M_1152


title: 10C_M_1151


title: 10C_M_1154


title: 10C_M_1150


title: 10C_M_1155


title: 10C_M_115


title: 10C_M_1176


title: 10C_M_1148


title: 10C_M_1153


title: 10C_M_1149


title: 10C_M_1146


title: 10C_M_1145


title: 10C_M_1143


title: 10C_M_1147


title: 10C_M_1141


title: 10C_M_1139


title: 10C_M_1140


title: 10C_M_114


title: 10C_M_1137


title: 10C_M_1136


title: 10C_M_1132


title: 10C_M_1134


title: 10C_M_1130


title: 10C_M_1133


title: 10C_M_113


title: 10C_M_1131


title: 10C_M_1135


title: 10C_M_1127


title: 10C_M_1125


title: 10C_M_1129


title: 10C_M_1124


title: 10C_M_1126


title: 10C_M_1128


title: 10C_M_1123


title: 10C_M_1121


title: 10C_M_1119


title: 10C_M_1118


title: 10C_M_1120


title: 10C_M_1117


title: 10C_M_1122


title: 10C_M_1138


title: 10C_M_1114


title: 10C_M_1116


title: 10C_M_1113


title: 10C_M_1111


title: 10C_M_1110


title: 10C_M_111


title: 10C_M_1109


title: 10C_M_1108


title: 10C_M_1106


title: 10C_M_1105


title: 10C_M_1107


title: 10C_M_1101


title: 10C_M_1103


title: 10C_M_1102


title: 10C_M_1104


title: 10C_M_110


title: 10C_M_11


title: 10C_M_1099


title: 10C_M_1100


title: 10C_M_1093


title: 10C_M_1094


title: 10C_M_1115


title: 10C_M_1098


title: 10C_M_1142


title: 10C_M_1096


title: 10C_M_1092


title: 10C_M_1091


title: 10C_M_1090


title: 10C_M_109


title: 10C_M_1089


title: 10C_M_1112


title: 10C_M_1086


title: 10C_M_1084


title: 10C_M_1082


title: 10C_M_1080


title: 10C_M_1085


title: 10C_M_1081


title: 10C_M_1083


title: 10C_M_108


title: 10C_M_1079


title: 10C_M_1077


title: 10C_M_1097


title: 10C_M_1075


title: 10C_M_1095


title: 10C_M_1078


title: 10C_M_1076


title: 10C_M_1073


title: 10C_M_1074


title: 10C_M_1070


title: 10C_M_1067


title: 10C_M_1069


title: 10C_M_107


title: 10C_M_1065


title: 10C_M_1064


title: 10C_M_1066


title: 10C_M_1068


title: 10C_M_1063


title: 10C_M_1061


title: 10C_M_1060


title: 10C_M_106


title: 10C_M_1058


title: 10C_M_1059


title: 10C_M_1057


title: 10C_M_1056


title: 10C_M_1054


title: 10C_M_1051


title: 10C_M_1072


title: 10C_M_1071


title: 10C_M_1049


title: 10C_M_105


title: 10C_M_1048


title: 10C_M_1050


title: 10C_M_1045


title: 10C_M_1062


title: 10C_M_1044


title: 10C_M_1043


title: 10C_M_104


title: 10C_M_1042


title: 10C_M_1041


title: 10C_M_1039


title: 10C_M_1038


title: 10C_M_1033


title: 10C_M_1055


title: 10C_M_1034


title: 10C_M_1036


title: 10C_M_1035


title: 10C_M_1053


title: 10C_M_1031


title: 10C_M_1029


title: 10C_M_1052


title: 10C_M_1030


title: 10C_M_1046


title: 10C_M_1028


title: 10C_M_1027


title: 10C_M_1026


title: 10C_M_1047


title: 10C_M_1024


title: 10C_M_1023


title: 10C_M_1025


title: 10C_M_1020


title: 10C_M_1019


title: 10C_M_1015


title: 10C_M_1017


title: 10C_M_1016


title: 10C_M_1013


title: 10C_M_1018


title: 10C_M_1032


title: 10C_M_1009


title: 10C_M_101


title: 10C_M_1012


title: 10C_M_1037


title: 10C_M_1007


title: 10C_M_1010


title: 10C_M_1008


title: 10C_M_1006


title: 10C_M_1005


title: 10C_M_1003


title: 10C_M_1001


title: 10C_M_1022


title: 10C_M_1002


title: 10C_M_1004


title: 10C_M_1021


title: 10C_M_100


title: 10C_M_1000


title: 10B_M_2851


title: 10C_M_0


title: 10C_M_1


title: 10C_M_10


title: 10C_M_102


title: 10B_M_2850


title: 10B_M_2847


title: 10B_M_2845


title: 10B_M_2840


title: 10B_M_2839


title: 10B_M_2846


title: 10B_M_2838


title: 10B_M_2833


title: 10B_M_2830


title: 10B_M_2825


title: 10B_M_2822


title: 10B_M_2819


title: 10B_M_2832


title: 10B_M_2826


title: 10B_M_2818


title: 10B_M_2810


title: 10B_M_2809


title: 10B_M_2807


title: 10B_M_2811


title: 10B_M_2805


title: 10B_M_2812


title: 10B_M_2804


title: 10B_M_2815


title: 10B_M_2849


title: 10B_M_2800


title: 10B_M_2798


title: 10B_M_2799


title: 10B_M_2802


title: 10B_M_2797


title: 10B_M_2796


title: 10B_M_2789


title: 10B_M_2794


title: 10B_M_2792


title: 10B_M_2820


title: 10B_M_2787


title: 10B_M_2786


title: 10B_M_2784


title: 10B_M_2783


title: 10B_M_2781


title: 10B_M_2801


title: 10B_M_2773


title: 10B_M_2772


title: 10B_M_2777


title: 10B_M_2766


title: 10B_M_2764


title: 10B_M_2771


title: 10B_M_2776


title: 10B_M_2778


title: 10B_M_2769


title: 10B_M_2765


title: 10B_M_2755


title: 10B_M_2759


title: 10B_M_2790


title: 10B_M_2760


title: 10B_M_2751


title: 10B_M_2750


title: 10B_M_2749


title: 10B_M_2748


title: 10B_M_2753


title: 10B_M_2785


title: 10B_M_2747


title: 10B_M_2782


title: 10B_M_2744


title: 10B_M_2780


title: 10B_M_2741


title: 10B_M_2743


title: 10B_M_2779


title: 10B_M_2738


title: 10B_M_2757


title: 10B_M_2736


title: 10B_M_2735


title: 10B_M_2730


title: 10B_M_2732


title: 10B_M_2729


title: 10B_M_2733


title: 10B_M_2726


title: 10B_M_2734


title: 10B_M_2731


title: 10B_M_2727


title: 10B_M_2723


title: 10B_M_2721


title: 10B_M_2722


title: 10B_M_2720


title: 10B_M_2715


title: 10B_M_2709


title: 10B_M_2710


title: 10B_M_2707


title: 10B_M_2706


title: 10B_M_2740


title: 10B_M_2708


title: 10B_M_2724


title: 10B_M_2713


title: 10B_M_2711


title: 10B_M_2705


title: 10B_M_2704


title: 10B_M_2700


title: 10B_M_2703


title: 10B_M_2699


title: 10B_M_2697


title: 10B_M_2696


title: 10B_M_2694


title: 10B_M_2690


title: 10B_M_2689


title: 10B_M_2686


title: 10B_M_2693


title: 10B_M_2684


title: 10B_M_2687


title: 10B_M_2683


title: 10B_M_2675


title: 10B_M_2672


title: 10B_M_2674


title: 10B_M_2668


title: 10B_M_2669


title: 10B_M_2665


title: 10B_M_2671


title: 10B_M_2666


title: 10B_M_2677


title: 10B_M_2663


title: 10B_M_2660


title: 10B_M_2659


title: 10B_M_2657


title: 10B_M_2656


title: 10B_M_2664


title: 10B_M_2658


title: 10B_M_2654


title: 10B_M_2655


title: 10B_M_2652


title: 10B_M_2650


title: 10B_M_2648


title: 10B_M_2688


title: 10B_M_2649


title: 10B_M_2647


title: 10B_M_2645


title: 10B_M_2646


title: 10B_M_2644


title: 10B_M_2643


title: 10B_M_2642


title: 10B_M_2641


title: 10B_M_2640


title: 10B_M_2638


title: 10B_M_2636


title: 10B_M_2634


title: 10B_M_2633


title: 10B_M_2632


title: 10B_M_2631


title: 10B_M_2630


title: 10B_M_2629


title: 10B_M_2628


title: 10B_M_2627


title: 10B_M_2620


title: 10B_M_2616


title: 10B_M_2624


title: 10B_M_2622


title: 10B_M_2625


title: 10B_M_2615


title: 10B_M_2614


title: 10B_M_2613


title: 10B_M_2612


title: 10B_M_2610


title: 10B_M_2609


title: 10B_M_2621


title: 10B_M_2603


title: 10B_M_2606


title: 10B_M_2601


title: 10B_M_2600


title: 10B_M_2635


title: 10B_M_2599


title: 10B_M_2598


title: 10B_M_2597


title: 10B_M_2596


title: 10B_M_2595


title: 10B_M_2592


title: 10B_M_2593


title: 10B_M_2588


title: 10B_M_2589


title: 10B_M_2587


title: 10B_M_2580


title: 10B_M_2579


title: 10B_M_2575


title: 10B_M_2573


title: 10B_M_2572


title: 10B_M_2566


title: 10B_M_2567


title: 10B_M_2568


title: 10B_M_2571


title: 10B_M_2560


title: 10B_M_2562


title: 10B_M_2554


title: 10B_M_2558


title: 10B_M_2561


title: 10B_M_2549


title: 10B_M_2564


title: 10B_M_2548


title: 10B_M_2545


title: 10B_M_2543


title: 10B_M_2552


title: 10B_M_2547


title: 10B_M_2541


title: 10B_M_2591


title: 10B_M_2538


title: 10B_M_2536


title: 10B_M_2535


title: 10B_M_2537


title: 10B_M_2534


title: 10B_M_2531


title: 10B_M_2528


title: 10B_M_2525


title: 10B_M_2527


title: 10B_M_2522


title: 10B_M_2524


title: 10B_M_2526


title: 10B_M_2519


title: 10B_M_2517


title: 10B_M_2518


title: 10B_M_2542


title: 10B_M_2513


title: 10B_M_2511


title: 10B_M_2507


title: 10B_M_2509


title: 10B_M_2574


title: 10B_M_2506


title: 10B_M_2510


title: 10B_M_2540


title: 10B_M_2505


title: 10B_M_2503


title: 10B_M_2532


title: 10B_M_2521


title: 10B_M_2493


title: 10B_M_2500


title: 10B_M_2492


title: 10B_M_2491


title: 10B_M_2499


title: 10B_M_2490


title: 10B_M_2483


title: 10B_M_2485


title: 10B_M_2481


title: 10B_M_2489


title: 10B_M_2482


title: 10B_M_2514


title: 10B_M_2515


title: 10B_M_2478


title: 10B_M_2477


title: 10B_M_2471


title: 10B_M_2473


title: 10B_M_2470


title: 10B_M_2466


title: 10B_M_2468


title: 10B_M_2465


title: 10B_M_2461


title: 10B_M_2469


title: 10B_M_2455


title: 10B_M_2459


title: 10B_M_2463


title: 10B_M_2454


title: 10B_M_2460


title: 10B_M_2451


title: 10B_M_2480


title: 10B_M_2446


title: 10B_M_2450


title: 10B_M_2448


title: 10B_M_2444


title: 10B_M_2437


title: 10B_M_2439


title: 10B_M_2440


title: 10B_M_2436


title: 10B_M_2435


title: 10B_M_2430


title: 10B_M_2428


title: 10B_M_2462


title: 10B_M_2433


title: 10B_M_2425


title: 10B_M_2427


title: 10B_M_2423


title: 10B_M_2422


title: 10B_M_2420


title: 10B_M_2421


title: 10B_M_2447


title: 10B_M_2418


title: 10B_M_2424


title: 10B_M_2417


title: 10B_M_2415


title: 10B_M_2438


title: 10B_M_2445


title: 10B_M_2409


title: 10B_M_2431


title: 10B_M_2411


title: 10B_M_2429


title: 10B_M_2405


title: 10B_M_2404


title: 10B_M_2402


title: 10B_M_2407


title: 10B_M_2400


title: 10B_M_2406


title: 10B_M_2413


title: 10B_M_2396


title: 10B_M_2399


title: 10B_M_2398


title: 10B_M_2410


title: 10B_M_2388


title: 10B_M_2390


title: 10B_M_2408


title: 10B_M_2385


title: 10B_M_2391


title: 10B_M_2384


title: 10B_M_2377


title: 10B_M_2375


title: 10B_M_2376


title: 10B_M_2379


title: 10B_M_2374


title: 10B_M_2372


title: 10B_M_2368


title: 10B_M_2371


title: 10B_M_2370


title: 10B_M_2367


title: 10B_M_2387


title: 10B_M_2393


title: 10B_M_2395


title: 10B_M_2365


title: 10B_M_2361


title: 10B_M_2358


title: 10B_M_2356


title: 10B_M_2392


title: 10B_M_2360


title: 10B_M_2362


title: 10B_M_2352


title: 10B_M_2349


title: 10B_M_2353


title: 10B_M_2350


title: 10B_M_2347


title: 10B_M_2354


title: 10B_M_2351


title: 10B_M_2346


title: 10B_M_2345


title: 10B_M_2338


title: 10B_M_2341


title: 10B_M_2340


title: 10B_M_2336


title: 10B_M_2334


title: 10B_M_2328


title: 10B_M_2330


title: 10B_M_2323


title: 10B_M_2327


title: 10B_M_2318


title: 10B_M_2325


title: 10B_M_2316


title: 10B_M_2313


title: 10B_M_2319


title: 10B_M_2312


title: 10B_M_2314


title: 10B_M_2322


title: 10B_M_2309


title: 10B_M_2305


title: 10B_M_2306


title: 10B_M_2303


title: 10B_M_2301


title: 10B_M_2302


title: 10B_M_2300


title: 10B_M_2304


title: 10B_M_2344


title: 10B_M_2299


title: 10B_M_2295


title: 10B_M_2298


title: 10B_M_2289


title: 10B_M_2291


title: 10B_M_2324


title: 10B_M_2286


title: 10B_M_2290


title: 10B_M_2275


title: 10B_M_2276


title: 10B_M_2280


title: 10B_M_2270


title: 10B_M_2265


title: 10B_M_2279


title: 10B_M_2262


title: 10B_M_2282


title: 10B_M_2273


title: 10B_M_2277


title: 10B_M_2294


title: 10B_M_2260


title: 10B_M_2292


title: 10B_M_2278


title: 10B_M_2253


title: 10B_M_2263


title: 10B_M_2257


title: 10B_M_2249


title: 10B_M_2246


title: 10B_M_2259


title: 10B_M_2247


title: 10B_M_2256


title: 10B_M_2243


title: 10B_M_2254


title: 10B_M_2258


title: 10B_M_2238


title: 10B_M_2236


title: 10B_M_2250


title: 10B_M_2233


title: 10B_M_2248


title: 10B_M_2226


title: 10B_M_2228


title: 10B_M_2252


title: 10B_M_2269


title: 10B_M_2220


title: 10B_M_2245


title: 10B_M_2241


title: 10B_M_2234


title: 10B_M_2231


title: 10B_M_2229


title: 10B_M_2230


title: 10B_M_2206


title: 10B_M_2219


title: 10B_M_2221


title: 10B_M_2222


title: 10B_M_2225


title: 10B_M_2201


title: 10B_M_2200


title: 10B_M_2199


title: 10B_M_2195


title: 10B_M_2194


title: 10B_M_2218


title: 10B_M_2188


title: 10B_M_2216


title: 10B_M_2215


title: 10B_M_2239


title: 10B_M_2214


title: 10B_M_2211


title: 10B_M_2185


title: 10B_M_2209


title: 10B_M_2205


title: 10B_M_2184


title: 10B_M_2175


title: 10B_M_2173


title: 10B_M_2170


title: 10B_M_2168


title: 10B_M_2204


title: 10B_M_2196


title: 10B_M_2171


title: 10B_M_2197


title: 10B_M_2193


title: 10B_M_2164


title: 10B_M_2192


title: 10B_M_2187


title: 10B_M_2191


title: 10B_M_2208


title: 10B_M_2163


title: 10B_M_2161


title: 10B_M_2158


title: 10B_M_2151


title: 10B_M_2153


title: 10B_M_2180


title: 10B_M_2179


title: 10B_M_2177


title: 10B_M_2172


title: 10B_M_2203


title: 10B_M_2166


title: 10B_M_2167


title: 10B_M_2162


title: 10B_M_2141


title: 10B_M_2140


title: 10B_M_2137


title: 10B_M_2159


title: 10B_M_2147


title: 10B_M_2136


title: 10B_M_2135


title: 10B_M_2156


title: 10B_M_2139


title: 10B_M_2127


title: 10B_M_2122


title: 10B_M_2148


title: 10B_M_2150


title: 10B_M_2128


title: 10B_M_2123


title: 10B_M_2126


title: 10B_M_2132


title: 10B_M_2117


title: 10B_M_2119


title: 10B_M_2120


title: 10B_M_2109


title: 10B_M_2112


title: 10B_M_2107


title: 10B_M_2104


title: 10B_M_2100


title: 10B_M_2099


title: 10B_M_2105


title: 10B_M_2095


title: 10B_M_2097


title: 10B_M_2093


title: 10B_M_2091


title: 10B_M_2087


title: 10B_M_2090


title: 10B_M_2088


title: 10B_M_2089


title: 10B_M_2092


title: 10B_M_2085


title: 10B_M_2086


title: 10B_M_2083


title: 10B_M_2082


title: 10B_M_2084


title: 10B_M_2081


title: 10B_M_2079


title: 10B_M_2080


title: 10B_M_2074


title: 10B_M_2078


title: 10B_M_2076


title: 10B_M_2108


title: 10B_M_2072


title: 10B_M_2069


title: 10B_M_2066


title: 10B_M_2067


title: 10B_M_2062


title: 10B_M_2060


title: 10B_M_2065


title: 10B_M_2063


title: 10B_M_2047


title: 10B_M_2051


title: 10B_M_2059


title: 10B_M_2056


title: 10B_M_2057


title: 10B_M_2055


title: 10B_M_2048


title: 10B_M_2053


title: 10B_M_2046


title: 10B_M_2045


title: 10B_M_2052


title: 10B_M_2044


title: 10B_M_2043


title: 10B_M_2041


title: 10B_M_2042


title: 10B_M_2039


title: 10B_M_2030


title: 10B_M_2032


title: 10B_M_2033


title: 10B_M_2037


title: 10B_M_2036


title: 10B_M_2038


title: 10B_M_2029


title: 10B_M_2061


title: 10B_M_2019


title: 10B_M_2022


title: 10B_M_2016


title: 10B_M_2015


title: 10B_M_2008


title: 10B_M_2007


title: 10B_M_2010


title: 10B_M_2006


title: 10B_M_2001


title: 10B_M_2009


title: 10B_M_2003


title: 10B_M_2011


title: 10B_M_1996


title: 10B_M_1997


title: 10B_M_1998


title: 10B_M_1991


title: 10B_M_1985


title: 10B_M_1987


title: 10B_M_1986


title: 10B_M_1989


title: 10B_M_1995


title: 10B_M_2012


title: 10B_M_2028


title: 10B_M_1979


title: 10B_M_1970


title: 10B_M_1976


title: 10B_M_1982


title: 10B_M_1975


title: 10B_M_1980


title: 10B_M_2004


title: 10B_M_1999


title: 10B_M_2002


title: 10B_M_1984


title: 10B_M_1962


title: 10B_M_1959


title: 10B_M_1958


title: 10B_M_1955


title: 10B_M_1951


title: 10B_M_1957


title: 10B_M_1956


title: 10B_M_1953


title: 10B_M_1950


title: 10B_M_1948


title: 10B_M_1967


title: 10B_M_1945


title: 10B_M_1943


title: 10B_M_1941


title: 10B_M_1940


title: 10B_M_1936


title: 10B_M_1939


title: 10B_M_1960


title: 10B_M_1938


title: 10B_M_1929


title: 10B_M_1933


title: 10B_M_1961


title: 10B_M_1932


title: 10B_M_1965


title: 10B_M_1928


title: 10B_M_1949


title: 10B_M_1917


title: 10B_M_1921


title: 10B_M_1913


title: 10B_M_1915


title: 10B_M_1912


title: 10B_M_1909


title: 10B_M_1908


title: 10B_M_1911


title: 10B_M_1914


title: 10B_M_1907


title: 10B_M_1903


title: 10B_M_1902


title: 10B_M_1901


title: 10B_M_1930


title: 10B_M_1931


title: 10B_M_1900


title: 10B_M_1896


title: 10B_M_1897


title: 10B_M_1898


title: 10B_M_1891


title: 10B_M_1895


title: 10B_M_1886


title: 10B_M_1889


title: 10B_M_1877


title: 10B_M_1876


title: 10B_M_1873


title: 10B_M_1906


title: 10B_M_1869


title: 10B_M_1868


title: 10B_M_1882


title: 10B_M_1865


title: 10B_M_1885


title: 10B_M_1879


title: 10B_M_1875


title: 10B_M_1860


title: 10B_M_1855


title: 10B_M_1862


title: 10B_M_1874


title: 10B_M_1870


title: 10B_M_1858


title: 10B_M_1854


title: 10B_M_1899


title: 10B_M_1853


title: 10B_M_1884


title: 10B_M_1851


title: 10B_M_1840


title: 10B_M_1847


title: 10B_M_1845


title: 10B_M_1838


title: 10B_M_1834


title: 10B_M_1835


title: 10B_M_1863


title: 10B_M_1836


title: 10B_M_1833


title: 10B_M_1848


title: 10B_M_1844


title: 10B_M_1830


title: 10B_M_1829


title: 10B_M_1827


title: 10B_M_1818


title: 10B_M_1828


title: 10B_M_1815


title: 10B_M_1831


title: 10B_M_1824


title: 10B_M_1819


title: 10B_M_1811


title: 10B_M_1832


title: 10B_M_1817


title: 10B_M_1813


title: 10B_M_1816


title: 10B_M_1849


title: 10B_M_1842


title: 10B_M_1809


title: 10B_M_1810


title: 10B_M_1808


title: 10B_M_1812


title: 10B_M_1801


title: 10B_M_1804


title: 10B_M_1806


title: 10B_M_1800


title: 10B_M_1799


title: 10B_M_1793


title: 10B_M_1798


title: 10B_M_1791


title: 10B_M_1794


title: 10B_M_1790


title: 10B_M_1792


title: 10B_M_1785


title: 10B_M_1784


title: 10B_M_1782


title: 10B_M_1821


title: 10B_M_1781


title: 10B_M_1779