title: Pool165_Plate2-2-E5-F16


title: Pool165_Plate2-2-E5-F4

status: Public on May 05 2023

type: SRA

city: La Jolla

first: Joseph

middle: R

last: Ecker

state: CA

address: 10010 North Torrey Pines Road

department: Genomic Analysis Laboratory

country: USA

institute: HHMI-Salk-Institute

library_source: genomic

data_processing:
Reads trimed with Cutadapt for adapters and low quality bases (Phred score >= 20)
The resulting files are mapped to in-silico bisulfite converted reference genome using Bismark.
The raw BAM file will be filtered using samtools to filter reads by MAPQ > 10 and use Picard to remove PCR duplicates
Tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls will be generated with in-house developing package using the filtered BAM files
Assembly: mm10
Supplementary files format and content: tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls. Columns are: 1. Chromosome, 2. Position, 3. Strand, 4. Cytosin Context, 5. methylated base call, 6. total mapped base call, 7. not used, place holder column
Library strategy: snmC-seq2 & 3

molecule: genomic DNA

organism: Mus musculus

source_name: brain

growth_protocol: All experimental procedures using live animals were approved by the Salk Institute Animal Care and Use Committee. The knock-in mouse line, R26R-CAG-loxp-stop-loxp-Sun1-sfGFP-Myc (INTACT) was used for most experiments4 and they were maintained on a C57BL/6J background. 42-49 day old adult male and female INTACT mice were used for the retrograde labeling experiment.

extract_protocol:
Approximately two weeks after the AAVretro injection, brains were extracted from the 56-63 day old INTACT mice, immediately submerged in ice-cold slicing buffer (2.5mM KCl, 0.5mM CaCl2, 7mM MgCl2, 1.25mM NaH2PO4, 110mM sucrose, 10mM glucose and 25mM NaHCO3) that was bubbled with carbogen, and sliced into 0.6 mm coronal sections starting from the frontal pole. From each AAVretro-injected brain, the slices were kept in the ice-cold dissection buffer from which selected brain regions (Supplementary Table 1) were manually dissected under a fluorescent dissecting microscope (Olympus SZX16), following the Allen Mouse Common Coordinate Framework (CCF), Reference Atlas, Version 3 (2015) (Extended Data Fig. 1). The dissected brain tissues were transferred to prelabeled microcentrifuge tubes, immediately frozen in dry ice, and subsequently stored at -80oC.   For each dissected brain region, samples from 2 males and 2 females were pooled separately as biological replicates for nuclei preparation. The 2-mL glass tissue dounce homogenizer and pestles (Sigma-Aldrich D8938-1SET) were pre-chilled on ice. Nuclei were prepared using a modified protocol as reported by Lacar et al., 201622. In summary, the frozen brain tissues were transferred to the dounce homogenizer with 1 mL ice-cold NIM buffer (0.25M sucrose, 25mM KCl, 5mM MgCl2, 10mM Tris-HCl (pH7.4), 1mM DTT (Sigma 646563), 10μl of protease inhibitor (Sigma P8340)), with 0.1% Triton X-100 and 5μM Hoechst 33342 (Invitrogen H3570), and gently homogenized on ice with the pestle 10-15 times. The homogenate was transferred to pre-chilled microcentrifuge tubes and centrifuged at 1000 rcf for 8 min at 4oC to pellet the nuclei. The pellet was resuspended in 1 mL ice-cold NIM buffer, and again centrifuged at 1000 rcf for 8 min at 4oC. The pellet was then resuspended in 450 μL of ice-cold NSB buffer (0.25M sucrose, 5mM MgCl2, 10mM Tris-HCl (pH7.4), 1mM DTT, 9ul of Protease inhibitor), and filtered through 40μM cell strainer. The filtered nuclei suspension was incubated on ice for at least 30 minutes with 50μl of nuclease-free BSA for at least 10 minutes, then incubated with GFP antibody, Alexa Fluor 488 (Invitrogen, A-21311) and anti-NeuN antibody (EMD Millipore MAB377) conjugated with Alexa Fluor 647 (Invitrogen A20173). GFP+/NeuN+ single nuclei were isolated using fluorescence-activated nuclei sorting (FANS) on a BD Influx sorter with 100μm nozzle, and sorted into 384-well plates preloaded with 2μl of digestion buffer for snmC-seq (20 mL digestion buffer consists of 10 mL M-digestion buffer (2×, Zymo D5021-9), 1 ml Proteinase K (20 mg, Zymo D3001-2-20), 9 mL water, and 10 µL unmethylated lambda DNA (100 pg/µL, Promega, D1521)). The collected plates were incubated at 50oC for 20 minutes then stored at -20 oC.
The bisulfite conversion and library preparation were performed following the detailed snmC-seq2 protocol as previously described (Luo, C. et al., Nat. Commun. 2018, 9(1):3824.) or snmC-seq3 protocol described in (Liu, H. et al., Biorxiv. 2023:2023-04.). The libraries were sequenced on Illumina Novaseq 6000 using the S4 flow cell 2 x 150 bp mode.

treatment_protocol: To label neurons projecting to regions of interest, injections of rAAV2-retro-Cre (produced by Salk Vector Core or Vigene, 2x1012 to 1x1013 viral genomes/ml, produced with capsid from Addgene plasmid #81070 packaging pAAV-EF1a-Cre from Addgene plasmid #55636) were made into both hemispheres of the INTACT mice. Animals were anesthetized with either ketamine/xylazine or isoflurane, placed in a stereotaxic frame, and 0.1 to 0.5 microliters of AAV was injected by pressure into stereotaxic coordinates corresponding to the desired projection target.

tag: tissue

value: brain

tag: source

value: MOB

tag: target

value: ENT

tag: Sex

value: male


title: Pool165_Plate2-2-E5-F15


title: Pool165_Plate2-2-E5-A15


title: Pool165_Plate2-1-E5-O2

value: PTLp


title: Pool165_Plate2-1-E5-O14


title: Pool165_Plate2-1-E5-P13


title: Pool165_Plate2-2-E5-H3


title: Pool165_Plate2-1-E5-O13


title: Pool165_Plate2-1-E5-N14


title: Pool165_Plate2-1-E5-N13


title: Pool165_Plate2-2-E5-E4


title: Pool165_Plate2-1-E5-L14


title: Pool165_Plate2-1-E5-O1


title: Pool165_Plate2-1-E5-L13


title: Pool165_Plate2-1-E5-K13


title: Pool165_Plate2-1-E5-J2


title: Pool165_Plate2-2-E5-E15


title: Pool165_Plate2-1-E5-K1


title: Pool165_Plate2-1-E5-K2


title: Pool165_Plate2-1-E5-I1


title: Pool165_Plate2-2-E5-G3


title: Pool165_Plate2-1-E5-J13


title: Pool165_Plate2-1-E5-J14


title: Pool165_Plate2-1-E5-N2


title: Pool165_Plate2-1-E5-J1


title: Pool165_Plate2-2-E5-D15


title: Pool165_Plate2-1-E5-I2


title: Pool165_Plate2-1-E5-G2


title: Pool165_Plate2-1-E5-H1


title: Pool165_Plate2-1-E5-I14


title: Pool165_Plate2-1-E5-I13


title: Pool165_Plate2-1-E5-G13


title: Pool165_Plate2-1-E5-F2


title: Pool165_Plate2-1-E5-F13


title: Pool165_Plate2-1-E5-F1


title: Pool165_Plate2-1-E5-E13


title: Pool165_Plate2-1-E5-D1


title: Pool165_Plate2-1-E5-F14


title: Pool165_Plate2-1-E5-E14


title: Pool165_Plate2-1-E5-E2


title: Pool165_Plate2-1-E5-C1


title: Pool165_Plate2-1-E5-C13


title: Pool165_Plate2-1-E5-C2


title: Pool165_Plate2-1-E5-D13


title: Pool165_Plate2-1-E5-C14


title: Pool165_Plate2-1-E5-B13


title: Pool165_Plate2-1-E5-B1


title: Pool165_Plate2-1-E5-B2


title: Pool165_Plate2-1-E5-A14


title: Pool165_Plate2-1-E5-A2


title: Pool165_Plate2-1-E5-A13


title: Pool165_Plate1-6-I3-P24

value: female


title: Pool165_Plate2-1-E5-A1


title: Pool165_Plate1-6-I3-P23


title: Pool165_Plate1-6-I3-O24


title: Pool165_Plate1-6-I3-M24


title: Pool165_Plate1-6-I3-K23


title: Pool165_Plate1-6-I3-J24


title: Pool165_Plate1-6-I3-M11

value: RSP


title: Pool165_Plate1-6-I3-N11


title: Pool165_Plate1-6-I3-K11


title: Pool165_Plate1-6-I3-I23


title: Pool165_Plate1-6-I3-J11


title: Pool165_Plate1-6-I3-J23


title: Pool165_Plate1-6-I3-H23


title: Pool165_Plate1-6-I3-H12


title: Pool165_Plate1-6-I3-H11


title: Pool165_Plate1-6-I3-G12


title: Pool165_Plate1-6-I3-F24


title: Pool165_Plate1-6-I3-O12


title: Pool165_Plate1-6-I3-O23


title: Pool165_Plate1-6-I3-C24


title: Pool165_Plate1-6-I3-C11


title: Pool165_Plate1-6-I3-D12


title: Pool165_Plate1-6-I3-C12


title: Pool165_Plate1-6-I3-E24


title: Pool165_Plate1-6-I3-B23


title: Pool165_Plate1-6-I3-N24


title: Pool165_Plate1-6-I3-I11


title: Pool165_Plate1-6-I3-M12


title: Pool165_Plate2-2-E5-D16


title: Pool165_Plate1-6-I3-H24


title: Pool165_Plate1-6-I3-F11


title: Pool165_Plate1-5-I3-I9


title: Pool165_Plate1-5-I3-J21


title: Pool165_Plate1-5-I3-J10


title: Pool165_Plate1-5-I3-M10


title: Pool165_Plate1-5-I3-M21


title: Pool165_Plate1-5-I3-O9


title: Pool165_Plate1-6-I3-A24


title: Pool165_Plate1-5-I3-P21


title: Pool165_Plate1-6-I3-A23


title: Pool165_Plate1-5-I3-O22


title: Pool165_Plate1-6-I3-A11


title: Pool165_Plate1-5-I3-P22


title: Pool165_Plate1-5-I3-K10


title: Pool165_Plate1-5-I3-N10


title: Pool165_Plate1-5-I3-E21


title: Pool165_Plate1-5-I3-B9


title: Pool165_Plate1-5-I3-F21


title: Pool165_Plate1-5-I3-D9


title: Pool165_Plate1-5-I3-G10


title: Pool165_Plate1-5-I3-I21


title: Pool165_Plate1-5-I3-A21


title: Pool165_Plate1-5-I3-H9


title: Pool165_Plate1-5-I3-D21


title: Pool165_Plate1-5-I3-F9


title: Pool165_Plate1-5-I3-P10


title: Pool165_Plate1-4-I3-P7


title: Pool165_Plate1-4-I3-O20


title: Pool165_Plate1-5-I3-M9


title: Pool165_Plate1-4-I3-M20


title: Pool165_Plate1-4-I3-N19


title: Pool165_Plate1-4-I3-L7


title: Pool165_Plate1-4-I3-K19


title: Pool165_Plate1-4-I3-I7


title: Pool165_Plate1-4-I3-J7


title: Pool165_Plate1-4-I3-B20


title: Pool165_Plate1-4-I3-B19


title: Pool165_Plate1-4-I3-H8


title: Pool165_Plate1-4-I3-A19


title: Pool165_Plate1-3-I3-K17


title: Pool165_Plate1-3-I3-K5

value: ACA


title: Pool165_Plate1-4-I3-D7


title: Pool165_Plate1-4-I3-A7


title: Pool165_Plate1-4-I3-D20


title: Pool165_Plate1-3-I3-M6


title: Pool165_Plate1-3-I3-M18


title: Pool165_Plate1-3-I3-L5


title: Pool165_Plate1-3-I3-P18


title: Pool165_Plate1-4-I3-J19


title: Pool165_Plate1-3-I3-J17


title: Pool165_Plate1-4-I3-F7


title: Pool165_Plate1-4-I3-B8


title: Pool165_Plate1-4-I3-C19


title: Pool165_Plate1-3-I3-P6


title: Pool165_Plate1-3-I3-J5


title: Pool165_Plate1-3-I3-I6


title: Pool165_Plate1-3-I3-N18


title: Pool165_Plate1-3-I3-I17


title: Pool165_Plate1-3-I3-G6


title: Pool165_Plate1-3-I3-G17


title: Pool165_Plate1-4-I3-E7


title: Pool165_Plate1-3-I3-E5


title: Pool165_Plate1-3-I3-B17


title: Pool165_Plate1-2-I3-L3


title: Pool165_Plate1-2-I3-O3


title: Pool165_Plate1-2-I3-N16


title: Pool165_Plate1-3-I3-C5


title: Pool165_Plate1-3-I3-E17


title: Pool165_Plate1-3-I3-A17


title: Pool165_Plate1-2-I3-O16


title: Pool165_Plate1-2-I3-L4


title: Pool165_Plate1-2-I3-N3


title: Pool165_Plate1-3-I3-A18


title: Pool165_Plate1-2-I3-M4


title: Pool165_Plate1-2-I3-P4


title: Pool165_Plate1-3-I3-C17


title: Pool165_Plate1-2-I3-N4


title: Pool165_Plate1-2-I3-J15


title: Pool165_Plate1-2-I3-J4


title: Pool165_Plate1-2-I3-J3


title: Pool165_Plate1-2-I3-M3


title: Pool165_Plate1-2-I3-K4


title: Pool165_Plate1-2-I3-I15


title: Pool165_Plate1-2-I3-H15


title: Pool165_Plate1-3-I3-D18


title: Pool165_Plate1-2-I3-H16


title: Pool165_Plate1-3-I3-F5


title: Pool165_Plate1-3-I3-D17


title: Pool165_Plate1-2-I3-H3


title: Pool165_Plate1-2-I3-L16


title: Pool165_Plate1-2-I3-C4


title: Pool165_Plate1-2-I3-D16


title: Pool165_Plate1-2-I3-F15


title: Pool165_Plate1-2-I3-G4


title: Pool165_Plate1-2-I3-F16


title: Pool165_Plate1-2-I3-E4


title: Pool165_Plate1-2-I3-A4


title: Pool165_Plate1-2-I3-E16


title: Pool165_Plate1-2-I3-D3


title: Pool165_Plate1-2-I3-G16


title: Pool165_Plate1-2-I3-G15


title: Pool165_Plate1-2-I3-E15


title: Pool165_Plate1-2-I3-F3


title: Pool165_Plate1-2-I3-F4


title: Pool165_Plate1-2-I3-B15


title: Pool165_Plate1-2-I3-D4


title: Pool165_Plate1-1-I3-P2


title: Pool165_Plate1-2-I3-E3


title: Pool165_Plate1-2-I3-C15


title: Pool165_Plate1-1-I3-P14


title: Pool165_Plate1-1-I3-O14


title: Pool165_Plate1-1-I3-N2


title: Pool165_Plate1-1-I3-L2


title: Pool165_Plate1-1-I3-L14


title: Pool165_Plate1-1-I3-K2


title: Pool165_Plate1-2-I3-B4


title: Pool165_Plate1-1-I3-J14


title: Pool165_Plate1-1-I3-J13


title: Pool165_Plate1-1-I3-J2


title: Pool165_Plate1-1-I3-H14


title: Pool165_Plate1-1-I3-F13


title: Pool165_Plate1-1-I3-E14


title: Pool165_Plate1-1-I3-E13


title: Pool155_Plate4-6-A11-O23

value: AMY


title: Pool155_Plate4-6-A11-P23


title: Pool155_Plate4-6-A11-O24


title: Pool165_Plate1-1-I3-D1


title: Pool155_Plate4-6-A11-P12


title: Pool155_Plate4-6-A11-O12


title: Pool165_Plate1-1-I3-A14


title: Pool155_Plate4-6-A11-N24


title: Pool155_Plate4-6-A11-P24


title: Pool165_Plate1-1-I3-C13


title: Pool165_Plate1-1-I3-B14


title: Pool155_Plate4-6-A11-M12


title: Pool155_Plate4-6-A11-M24


title: Pool155_Plate4-6-A11-N12


title: Pool155_Plate4-6-A11-M23


title: Pool165_Plate1-2-I3-H4


title: Pool155_Plate4-6-A11-L24


title: Pool155_Plate4-6-A11-L12


title: Pool155_Plate4-6-A11-K23


title: Pool155_Plate4-6-A11-K24


title: Pool165_Plate1-1-I3-D14


title: Pool155_Plate4-6-A11-K12


title: Pool155_Plate4-6-A11-O11


title: Pool155_Plate4-6-A11-J23


title: Pool155_Plate4-6-A11-K11


title: Pool155_Plate4-6-A11-J11


title: Pool155_Plate4-6-A11-J24


title: Pool155_Plate4-6-A11-J12


title: Pool165_Plate1-1-I3-C14


title: Pool155_Plate4-6-A11-I24


title: Pool155_Plate4-6-A11-I12


title: Pool155_Plate4-6-A11-I23


title: Pool155_Plate4-6-A11-I11


title: Pool155_Plate4-6-A11-G12


title: Pool155_Plate4-6-A11-H11


title: Pool155_Plate4-6-A11-G24


title: Pool155_Plate4-6-A11-G23


title: Pool155_Plate4-6-A11-F23


title: Pool155_Plate4-6-A11-F12


title: Pool155_Plate4-6-A11-G11


title: Pool155_Plate4-6-A11-F24


title: Pool155_Plate4-6-A11-E12


title: Pool155_Plate4-6-A11-E23


title: Pool155_Plate4-6-A11-D12


title: Pool155_Plate4-6-A11-D23


title: Pool155_Plate4-6-A11-H23


title: Pool155_Plate4-6-A11-C23


title: Pool155_Plate4-6-A11-D11


title: Pool155_Plate4-6-A11-C12


title: Pool155_Plate4-6-A11-C11


title: Pool155_Plate4-6-A11-B24


title: Pool155_Plate4-6-A11-B23


title: Pool155_Plate4-6-A11-C24


title: Pool155_Plate4-6-A11-B12


title: Pool155_Plate4-6-A11-B11


title: Pool155_Plate4-6-A11-A24


title: Pool155_Plate4-6-A11-E11


title: Pool155_Plate4-6-A11-A12


title: Pool155_Plate4-5-A11-O9


title: Pool155_Plate4-5-A11-P21


title: Pool155_Plate4-5-A11-P22


title: Pool155_Plate4-6-A11-A11


title: Pool155_Plate4-5-A11-M10


title: Pool155_Plate4-5-A11-M22


title: Pool155_Plate4-5-A11-L22


title: Pool155_Plate4-5-A11-M9


title: Pool155_Plate4-5-A11-O21


title: Pool155_Plate4-5-A11-M21


title: Pool155_Plate4-5-A11-L10


title: Pool155_Plate4-5-A11-L9


title: Pool155_Plate4-5-A11-N21


title: Pool155_Plate4-5-A11-O10


title: Pool155_Plate4-5-A11-O22


title: Pool155_Plate4-5-A11-K9


title: Pool155_Plate4-5-A11-K22


title: Pool155_Plate4-5-A11-K21


title: Pool155_Plate4-5-A11-J9


title: Pool155_Plate4-5-A11-K10


title: Pool155_Plate4-5-A11-L21


title: Pool155_Plate4-5-A11-J21


title: Pool155_Plate4-5-A11-J22


title: Pool155_Plate4-5-A11-J10


title: Pool155_Plate4-5-A11-I9


title: Pool155_Plate4-5-A11-H9


title: Pool155_Plate4-5-A11-I10


title: Pool155_Plate4-5-A11-I22


title: Pool155_Plate4-5-A11-G9


title: Pool155_Plate4-5-A11-H10


title: Pool155_Plate4-5-A11-G10


title: Pool155_Plate4-5-A11-F9


title: Pool155_Plate4-5-A11-G22


title: Pool155_Plate4-5-A11-E22


title: Pool155_Plate4-5-A11-E9


title: Pool155_Plate4-5-A11-E21


title: Pool155_Plate4-5-A11-F21


title: Pool155_Plate4-5-A11-F10


title: Pool155_Plate4-5-A11-D21


title: Pool155_Plate4-5-A11-C10


title: Pool155_Plate4-5-A11-C22


title: Pool155_Plate4-5-A11-B9


title: Pool155_Plate4-5-A11-C9


title: Pool155_Plate4-5-A11-F22


title: Pool155_Plate4-5-A11-C21


title: Pool155_Plate4-5-A11-I21


title: Pool155_Plate4-5-A11-D9


title: Pool155_Plate4-5-A11-B22


title: Pool155_Plate4-5-A11-D22


title: Pool155_Plate4-5-A11-B10


title: Pool155_Plate4-5-A11-A9


title: Pool155_Plate4-5-A11-A22


title: Pool155_Plate4-5-A11-A21


title: Pool155_Plate4-5-A11-A10


title: Pool155_Plate4-4-A11-P20


title: Pool155_Plate4-5-A11-B21


title: Pool155_Plate4-4-A11-O19


title: Pool155_Plate4-4-A11-N7


title: Pool155_Plate4-4-A11-O7


title: Pool155_Plate4-4-A11-L8


title: Pool155_Plate4-4-A11-L7


title: Pool155_Plate4-4-A11-L19


title: Pool155_Plate4-4-A11-K8


title: Pool155_Plate4-4-A11-K20


title: Pool155_Plate4-4-A11-J20


title: Pool155_Plate4-4-A11-J19


title: Pool155_Plate4-4-A11-J8


title: Pool155_Plate4-4-A11-J7


title: Pool155_Plate4-4-A11-I8


title: Pool155_Plate4-4-A11-I7


title: Pool155_Plate4-4-A11-N8


title: Pool155_Plate4-4-A11-I20


title: Pool155_Plate4-4-A11-M19


title: Pool155_Plate4-4-A11-H19


title: Pool155_Plate4-4-A11-H20


title: Pool155_Plate4-4-A11-H8


title: Pool155_Plate4-4-A11-G7


title: Pool155_Plate4-4-A11-G8


title: Pool155_Plate4-4-A11-L20


title: Pool155_Plate4-4-A11-K7


title: Pool155_Plate4-4-A11-K19


title: Pool155_Plate4-4-A11-G20


title: Pool155_Plate4-4-A11-E8


title: Pool155_Plate4-4-A11-F20


title: Pool155_Plate4-4-A11-F7


title: Pool155_Plate4-4-A11-F8


title: Pool155_Plate4-4-A11-B20


title: Pool155_Plate4-4-A11-D7


title: Pool155_Plate4-4-A11-B8


title: Pool155_Plate4-4-A11-B19


title: Pool155_Plate4-4-A11-D8


title: Pool155_Plate4-4-A11-C7


title: Pool155_Plate4-4-A11-C8


title: Pool155_Plate4-4-A11-A8


title: Pool155_Plate4-4-A11-D19


title: Pool155_Plate4-4-A11-D20


title: Pool155_Plate4-4-A11-E19


title: Pool155_Plate4-4-A11-A7


title: Pool155_Plate4-4-A11-G19


title: Pool155_Plate4-4-A11-A19


title: Pool155_Plate4-3-A11-P5


title: Pool155_Plate4-4-A11-E20


title: Pool155_Plate4-3-A11-P17


title: Pool155_Plate4-3-A11-P18


title: Pool155_Plate4-3-A11-O18


title: Pool155_Plate4-3-A11-O6


title: Pool155_Plate4-3-A11-O17


title: Pool155_Plate4-3-A11-L6


title: Pool155_Plate4-3-A11-M17


title: Pool155_Plate4-3-A11-M18


title: Pool155_Plate4-3-A11-L5


title: Pool155_Plate4-3-A11-K6


title: Pool155_Plate4-3-A11-L17


title: Pool155_Plate4-3-A11-K5


title: Pool155_Plate4-3-A11-L18


title: Pool155_Plate4-3-A11-K18


title: Pool155_Plate4-3-A11-J17


title: Pool155_Plate4-3-A11-J5


title: Pool155_Plate4-3-A11-J18


title: Pool155_Plate4-3-A11-M5


title: Pool155_Plate4-3-A11-H5


title: Pool155_Plate4-3-A11-H18


title: Pool155_Plate4-3-A11-H17


title: Pool155_Plate4-3-A11-H6


title: Pool155_Plate4-3-A11-G6


title: Pool155_Plate4-3-A11-I18


title: Pool155_Plate4-3-A11-G5


title: Pool155_Plate4-3-A11-I5


title: Pool155_Plate4-3-A11-E5


title: Pool155_Plate4-3-A11-E6


title: Pool155_Plate4-3-A11-F5


title: Pool155_Plate4-3-A11-G17


title: Pool155_Plate4-3-A11-F6


title: Pool155_Plate4-3-A11-F17


title: Pool155_Plate4-3-A11-F18


title: Pool155_Plate4-3-A11-K17


title: Pool155_Plate4-3-A11-J6


title: Pool155_Plate4-3-A11-I17


title: Pool155_Plate4-3-A11-D17


title: Pool155_Plate4-3-A11-E17


title: Pool155_Plate4-3-A11-D5


title: Pool155_Plate4-3-A11-C6


title: Pool155_Plate4-3-A11-I6


title: Pool155_Plate4-3-A11-C18


title: Pool155_Plate4-3-A11-C17


title: Pool155_Plate4-3-A11-B5


title: Pool155_Plate4-3-A11-B17


title: Pool155_Plate4-3-A11-B6


title: Pool155_Plate4-2-A11-P3


title: Pool155_Plate4-3-A11-A18


title: Pool155_Plate4-3-A11-A5


title: Pool155_Plate4-3-A11-E18


title: Pool155_Plate4-3-A11-D6


title: Pool155_Plate4-2-A11-P16


title: Pool155_Plate4-2-A11-O3


title: Pool155_Plate4-2-A11-P15


title: Pool155_Plate4-3-A11-G18


title: Pool155_Plate4-2-A11-O16


title: Pool155_Plate4-3-A11-B18


title: Pool155_Plate4-2-A11-O4


title: Pool155_Plate4-2-A11-I4


title: Pool155_Plate4-2-A11-I16


title: Pool155_Plate4-2-A11-K3


title: Pool155_Plate4-2-A11-K4


title: Pool155_Plate4-2-A11-L15


title: Pool155_Plate4-2-A11-L4


title: Pool155_Plate4-2-A11-I15


title: Pool155_Plate4-2-A11-J15


title: Pool155_Plate4-2-A11-G16


title: Pool155_Plate4-2-A11-M15


title: Pool155_Plate4-2-A11-H16


title: Pool155_Plate4-2-A11-L3


title: Pool155_Plate4-2-A11-K15


title: Pool155_Plate4-2-A11-J16


title: Pool155_Plate4-2-A11-G4


title: Pool155_Plate4-2-A11-K16


title: Pool155_Plate4-2-A11-H4


title: Pool155_Plate4-2-A11-G15


title: Pool155_Plate4-2-A11-M3


title: Pool155_Plate4-2-A11-M4


title: Pool155_Plate4-2-A11-M16


title: Pool155_Plate4-2-A11-N15


title: Pool155_Plate4-2-A11-O15


title: Pool155_Plate4-2-A11-I3


title: Pool155_Plate4-2-A11-F4


title: Pool155_Plate4-2-A11-F16


title: Pool155_Plate4-2-A11-F15


title: Pool155_Plate4-2-A11-F3


title: Pool155_Plate4-2-A11-G3


title: Pool155_Plate4-2-A11-C4


title: Pool155_Plate4-2-A11-A16


title: Pool155_Plate4-2-A11-E4


title: Pool155_Plate4-2-A11-D4


title: Pool155_Plate4-2-A11-D15


title: Pool155_Plate4-2-A11-E16


title: Pool155_Plate4-2-A11-D16


title: Pool155_Plate4-2-A11-D3


title: Pool155_Plate4-1-A11-O14


title: Pool155_Plate4-1-A11-P14


title: Pool155_Plate4-1-A11-O2


title: Pool155_Plate4-1-A11-P2


title: Pool155_Plate4-2-A11-E15


title: Pool155_Plate4-1-A11-P1


title: Pool155_Plate4-2-A11-E3


title: Pool155_Plate4-2-A11-A3


title: Pool155_Plate4-2-A11-A15


title: Pool155_Plate4-2-A11-C16


title: Pool155_Plate4-2-A11-B3


title: Pool155_Plate4-2-A11-C15


title: Pool155_Plate4-2-A11-B15


title: Pool155_Plate4-1-A11-N14


title: Pool155_Plate4-1-A11-O13


title: Pool155_Plate4-1-A11-M2


title: Pool155_Plate4-1-A11-N13


title: Pool155_Plate4-1-A11-M14


title: Pool155_Plate4-1-A11-M13


title: Pool155_Plate4-2-A11-B16


title: Pool155_Plate4-1-A11-N1


title: Pool155_Plate4-1-A11-M1


title: Pool155_Plate4-1-A11-L2


title: Pool155_Plate4-1-A11-L14


title: Pool155_Plate4-1-A11-G13


title: Pool155_Plate4-1-A11-G14


title: Pool155_Plate4-1-A11-I2


title: Pool155_Plate4-1-A11-L1


title: Pool155_Plate4-1-A11-K14


title: Pool155_Plate4-1-A11-J14


title: Pool155_Plate4-1-A11-J13


title: Pool155_Plate4-1-A11-H2


title: Pool155_Plate4-1-A11-F13


title: Pool155_Plate4-1-A11-I13


title: Pool155_Plate4-1-A11-F14


title: Pool155_Plate4-1-A11-J1


title: Pool155_Plate4-1-A11-L13


title: Pool155_Plate4-1-A11-H14


title: Pool155_Plate4-1-A11-E1


title: Pool155_Plate4-1-A11-D2


title: Pool155_Plate4-1-A11-K1


title: Pool155_Plate4-1-A11-E13


title: Pool155_Plate4-1-A11-J2


title: Pool155_Plate4-1-A11-G1


title: Pool155_Plate4-1-A11-E14


title: Pool155_Plate4-1-A11-D14


title: Pool155_Plate4-1-A11-G2


title: Pool155_Plate4-1-A11-E2


title: Pool155_Plate4-1-A11-K13


title: Pool155_Plate4-1-A11-I14


title: Pool155_Plate4-1-A11-D13


title: Pool155_Plate4-1-A11-C2


title: Pool155_Plate4-1-A11-D1


title: Pool155_Plate4-1-A11-C13


title: Pool155_Plate4-1-A11-C14


title: Pool155_Plate4-1-A11-B14


title: Pool155_Plate4-1-A11-B13


title: Pool155_Plate4-1-A11-B2


title: Pool155_Plate4-1-A11-B1


title: Pool155_Plate4-1-A11-A1


title: Pool155_Plate3-6-O9-O24


title: Pool155_Plate4-1-A11-A14


title: Pool155_Plate3-6-O9-N24


title: Pool155_Plate3-6-O9-N23


title: Pool155_Plate4-1-A11-A13


title: Pool155_Plate3-6-O9-N12


title: Pool155_Plate3-6-O9-P23


title: Pool155_Plate3-6-O9-M23


title: Pool155_Plate3-6-O9-O23


title: Pool155_Plate3-6-O9-M24


title: Pool155_Plate3-6-O9-K24


title: Pool155_Plate3-6-O9-K23


title: Pool155_Plate3-6-O9-L23


title: Pool155_Plate3-6-O9-J24


title: Pool155_Plate3-6-O9-L11


title: Pool155_Plate3-6-O9-K12


title: Pool155_Plate3-6-O9-J23


title: Pool155_Plate3-6-O9-I23


title: Pool155_Plate3-6-O9-I12


title: Pool155_Plate3-6-O9-I24


title: Pool155_Plate4-1-A11-C1


title: Pool155_Plate3-6-O9-H23


title: Pool155_Plate3-6-O9-H11


title: Pool155_Plate3-6-O9-G24


title: Pool155_Plate3-6-O9-F24


title: Pool155_Plate3-6-O9-F23


title: Pool155_Plate3-6-O9-E12


title: Pool155_Plate3-6-O9-E23


title: Pool155_Plate3-6-O9-E24


title: Pool155_Plate4-2-A11-L16


title: Pool155_Plate3-6-O9-A24


title: Pool155_Plate3-6-O9-D24


title: Pool155_Plate3-6-O9-D23


title: Pool155_Plate3-5-O9-P22


title: Pool155_Plate3-6-O9-A23


title: Pool155_Plate3-6-O9-A12


title: Pool155_Plate3-5-O9-O22


title: Pool155_Plate3-5-O9-P21


title: Pool155_Plate3-5-O9-K9


title: Pool155_Plate3-5-O9-K21


title: Pool155_Plate3-5-O9-M21


title: Pool155_Plate3-5-O9-L22


title: Pool155_Plate3-5-O9-K22


title: Pool155_Plate3-5-O9-N22


title: Pool155_Plate3-5-O9-O10


title: Pool155_Plate3-5-O9-J9


title: Pool155_Plate3-6-O9-G23


title: Pool155_Plate3-6-O9-F11


title: Pool155_Plate3-5-O9-J22


title: Pool155_Plate3-6-O9-C24


title: Pool155_Plate3-5-O9-I9


title: Pool155_Plate3-5-O9-I21


title: Pool155_Plate3-5-O9-J21


title: Pool155_Plate3-5-O9-G9


title: Pool155_Plate3-5-O9-H21


title: Pool155_Plate3-5-O9-F21


title: Pool155_Plate3-5-O9-G21


title: Pool155_Plate3-5-O9-E21


title: Pool155_Plate3-5-O9-E22


title: Pool155_Plate3-5-O9-F10


title: Pool155_Plate3-4-O9-O19


title: Pool155_Plate3-4-O9-N7


title: Pool155_Plate3-4-O9-M8


title: Pool155_Plate3-4-O9-N20


title: Pool155_Plate3-5-O9-A21


title: Pool155_Plate3-4-O9-N19


title: Pool155_Plate3-5-O9-C9


title: Pool155_Plate3-5-O9-A9


title: Pool155_Plate3-4-O9-O8


title: Pool155_Plate3-4-O9-L20


title: Pool155_Plate3-4-O9-L8


title: Pool155_Plate3-4-O9-K20


title: Pool155_Plate3-4-O9-K7


title: Pool155_Plate3-4-O9-L7


title: Pool155_Plate3-4-O9-M19


title: Pool155_Plate3-4-O9-K8


title: Pool155_Plate3-4-O9-J8


title: Pool155_Plate3-4-O9-L19


title: Pool155_Plate3-4-O9-J7


title: Pool155_Plate3-4-O9-K19


title: Pool155_Plate3-4-O9-O20


title: Pool155_Plate3-4-O9-J20


title: Pool155_Plate3-4-O9-I8


title: Pool155_Plate3-4-O9-J19


title: Pool155_Plate3-4-O9-P7


title: Pool155_Plate3-4-O9-I7


title: Pool155_Plate3-4-O9-M7


title: Pool155_Plate3-4-O9-H7


title: Pool155_Plate3-4-O9-H20


title: Pool155_Plate3-4-O9-G8


title: Pool155_Plate3-4-O9-G7


title: Pool155_Plate3-4-O9-D7


title: Pool155_Plate3-4-O9-E7


title: Pool155_Plate3-4-O9-C8


title: Pool155_Plate3-4-O9-C7


title: Pool155_Plate3-4-O9-D20


title: Pool155_Plate3-4-O9-E20


title: Pool155_Plate3-4-O9-D19


title: Pool155_Plate3-4-O9-A7


title: Pool155_Plate3-4-O9-B8


title: Pool155_Plate3-4-O9-F20


title: Pool155_Plate3-4-O9-B19


title: Pool155_Plate3-4-O9-E8


title: Pool155_Plate3-4-O9-C20


title: Pool155_Plate3-4-O9-G20


title: Pool155_Plate3-4-O9-A20


title: Pool155_Plate3-4-O9-C19


title: Pool155_Plate3-3-O9-P6


title: Pool155_Plate3-4-O9-H8


title: Pool155_Plate3-4-O9-I19


title: Pool155_Plate3-4-O9-A19


title: Pool155_Plate3-3-O9-O6


title: Pool155_Plate3-3-O9-P5


title: Pool155_Plate3-3-O9-O17


title: Pool155_Plate3-3-O9-O5


title: Pool155_Plate3-4-O9-F8


title: Pool155_Plate3-3-O9-N5


title: Pool155_Plate3-3-O9-M18


title: Pool155_Plate3-4-O9-F7


title: Pool155_Plate3-3-O9-K17


title: Pool155_Plate3-3-O9-N18


title: Pool155_Plate3-3-O9-J6


title: Pool155_Plate3-3-O9-J5


title: Pool155_Plate3-3-O9-I18


title: Pool155_Plate3-3-O9-J17


title: Pool155_Plate3-3-O9-H6


title: Pool155_Plate3-3-O9-G6


title: Pool155_Plate3-3-O9-G5


title: Pool155_Plate3-3-O9-F18


title: Pool155_Plate3-3-O9-D6


title: Pool155_Plate3-3-O9-C5


title: Pool155_Plate3-3-O9-F6


title: Pool155_Plate3-3-O9-E5


title: Pool155_Plate3-3-O9-G18


title: Pool155_Plate3-3-O9-C17


title: Pool155_Plate3-3-O9-B6


title: Pool155_Plate3-3-O9-C18


title: Pool155_Plate3-3-O9-A6


title: Pool155_Plate3-2-O9-P3


title: Pool155_Plate3-3-O9-A17


title: Pool155_Plate3-3-O9-A18


title: Pool155_Plate3-2-O9-O4


title: Pool155_Plate3-2-O9-N3


title: Pool155_Plate3-3-O9-H18


title: Pool155_Plate3-3-O9-E6


title: Pool155_Plate3-2-O9-M3


title: Pool155_Plate3-2-O9-M4


title: Pool155_Plate3-3-O9-C6


title: Pool155_Plate3-2-O9-M16


title: Pool155_Plate3-2-O9-L4


title: Pool155_Plate3-2-O9-L15


title: Pool155_Plate3-2-O9-J4


title: Pool155_Plate3-2-O9-K4


title: Pool155_Plate3-2-O9-K15


title: Pool155_Plate3-2-O9-J3


title: Pool155_Plate3-3-O9-B5


title: Pool155_Plate3-2-O9-N4


title: Pool155_Plate3-2-O9-I3


title: Pool155_Plate3-2-O9-F4


title: Pool155_Plate3-2-O9-H15


title: Pool155_Plate3-2-O9-H4


title: Pool155_Plate3-2-O9-F3


title: Pool155_Plate3-2-O9-G15


title: Pool155_Plate3-2-O9-E4


title: Pool155_Plate3-2-O9-G4


title: Pool155_Plate3-2-O9-E3


title: Pool155_Plate3-2-O9-H16


title: Pool155_Plate3-2-O9-E15


title: Pool155_Plate3-2-O9-C3


title: Pool155_Plate3-2-O9-M15


title: Pool155_Plate3-2-O9-C4


title: Pool155_Plate3-2-O9-D4


title: Pool155_Plate3-2-O9-C15


title: Pool155_Plate3-1-O9-P1


title: Pool155_Plate3-1-O9-L2


title: Pool155_Plate3-1-O9-M2


title: Pool155_Plate3-2-O9-A3


title: Pool155_Plate3-1-O9-N14


title: Pool155_Plate3-1-O9-K2


title: Pool155_Plate3-1-O9-K13


title: Pool155_Plate3-1-O9-K1


title: Pool155_Plate3-1-O9-H14


title: Pool155_Plate3-1-O9-G2


title: Pool155_Plate3-2-O9-E16


title: Pool155_Plate3-1-O9-F1


title: Pool155_Plate3-1-O9-F2


title: Pool155_Plate3-1-O9-E2


title: Pool155_Plate2-6-M9-P12

value: PAG

value: PAL


title: Pool155_Plate3-1-O9-C1


title: Pool155_Plate3-1-O9-D2


title: Pool155_Plate3-1-O9-E1


title: Pool155_Plate3-1-O9-C13


title: Pool155_Plate2-6-M9-P24


title: Pool155_Plate3-1-O9-D1


title: Pool155_Plate2-6-M9-O12


title: Pool155_Plate2-6-M9-O23


title: Pool155_Plate3-1-O9-A2


title: Pool155_Plate2-6-M9-N24


title: Pool155_Plate2-6-M9-M24


title: Pool155_Plate2-6-M9-M23


title: Pool155_Plate3-1-O9-G14


title: Pool155_Plate2-6-M9-M12


title: Pool155_Plate2-6-M9-M11


title: Pool155_Plate2-6-M9-L24


title: Pool155_Plate2-6-M9-K24


title: Pool155_Plate2-6-M9-L23


title: Pool155_Plate2-6-M9-K23


title: Pool155_Plate3-1-O9-B14


title: Pool155_Plate2-6-M9-P23


title: Pool155_Plate2-6-M9-K12


title: Pool155_Plate2-6-M9-P11


title: Pool155_Plate2-6-M9-J24


title: Pool155_Plate2-6-M9-K11


title: Pool155_Plate2-6-M9-J23


title: Pool155_Plate2-6-M9-O11


title: Pool155_Plate2-6-M9-N23


title: Pool155_Plate2-6-M9-J12


title: Pool155_Plate2-6-M9-I24


title: Pool155_Plate2-6-M9-H23


title: Pool155_Plate2-6-M9-J11


title: Pool155_Plate2-6-M9-I23


title: Pool155_Plate2-6-M9-G24


title: Pool155_Plate2-6-M9-I12


title: Pool155_Plate2-6-M9-H12


title: Pool155_Plate2-6-M9-I11


title: Pool155_Plate2-6-M9-N11


title: Pool155_Plate2-6-M9-G12


title: Pool155_Plate2-6-M9-G23


title: Pool155_Plate2-6-M9-G11


title: Pool155_Plate2-6-M9-F24


title: Pool155_Plate2-6-M9-F23


title: Pool155_Plate2-6-M9-F11


title: Pool155_Plate2-6-M9-E24


title: Pool155_Plate2-6-M9-E23


title: Pool155_Plate2-6-M9-D24


title: Pool155_Plate2-6-M9-E11


title: Pool155_Plate2-6-M9-D23


title: Pool155_Plate2-6-M9-E12


title: Pool155_Plate2-6-M9-D12


title: Pool155_Plate2-6-M9-D11


title: Pool155_Plate2-6-M9-C24


title: Pool155_Plate2-6-M9-H24


title: Pool155_Plate2-6-M9-C23


title: Pool155_Plate2-6-M9-C12


title: Pool155_Plate2-6-M9-B24


title: Pool155_Plate2-6-M9-C11


title: Pool155_Plate2-6-M9-A23


title: Pool155_Plate2-6-M9-A12


title: Pool155_Plate2-6-M9-A24


title: Pool155_Plate2-6-M9-F12


title: Pool155_Plate2-6-M9-B23


title: Pool155_Plate2-6-M9-A11


title: Pool155_Plate2-6-M9-B11


title: Pool155_Plate2-5-M9-P9


title: Pool155_Plate2-5-M9-P10


title: Pool155_Plate2-5-M9-O9


title: Pool155_Plate2-5-M9-P21


title: Pool155_Plate2-5-M9-O10


title: Pool155_Plate2-5-M9-O22


title: Pool155_Plate2-5-M9-N9


title: Pool155_Plate2-5-M9-O21


title: Pool155_Plate2-6-M9-B12


title: Pool155_Plate2-5-M9-N21


title: Pool155_Plate2-5-M9-M22


title: Pool155_Plate2-5-M9-M10


title: Pool155_Plate2-5-M9-M21


title: Pool155_Plate2-5-M9-M9


title: Pool155_Plate2-5-M9-L9


title: Pool155_Plate2-5-M9-L21


title: Pool155_Plate2-5-M9-L22


title: Pool155_Plate2-5-M9-K9


title: Pool155_Plate2-5-M9-K22


title: Pool155_Plate2-5-M9-K21


title: Pool155_Plate2-5-M9-K10


title: Pool155_Plate2-5-M9-J22


title: Pool155_Plate2-5-M9-J9


title: Pool155_Plate2-5-M9-N22


title: Pool155_Plate2-5-M9-J21


title: Pool155_Plate2-5-M9-J10


title: Pool155_Plate2-5-M9-I10


title: Pool155_Plate2-5-M9-H22


title: Pool155_Plate2-5-M9-G9


title: Pool155_Plate2-5-M9-I9


title: Pool155_Plate2-5-M9-H9


title: Pool155_Plate2-5-M9-I21


title: Pool155_Plate2-5-M9-G21


title: Pool155_Plate2-5-M9-G22


title: Pool155_Plate2-5-M9-H21


title: Pool155_Plate2-5-M9-F22


title: Pool155_Plate2-5-M9-F21


title: Pool155_Plate2-5-M9-E21


title: Pool155_Plate2-5-M9-E22


title: Pool155_Plate2-5-M9-E9


title: Pool155_Plate2-5-M9-E10


title: Pool155_Plate2-5-M9-I22


title: Pool155_Plate2-5-M9-C22


title: Pool155_Plate2-5-M9-D9


title: Pool155_Plate2-5-M9-D21


title: Pool155_Plate2-5-M9-C9


title: Pool155_Plate2-5-M9-D10


title: Pool155_Plate2-5-M9-D22


title: Pool155_Plate2-5-M9-B9


title: Pool155_Plate2-5-M9-C10


title: Pool155_Plate2-5-M9-B22


title: Pool155_Plate2-5-M9-B21


title: Pool155_Plate2-5-M9-C21


title: Pool155_Plate2-5-M9-G10


title: Pool155_Plate2-4-M9-P8


title: Pool155_Plate2-5-M9-A22


title: Pool155_Plate2-5-M9-B10


title: Pool155_Plate2-5-M9-A9


title: Pool155_Plate2-5-M9-F10


title: Pool155_Plate2-4-M9-O19


title: Pool155_Plate2-4-M9-P19


title: Pool155_Plate2-4-M9-O7


title: Pool155_Plate2-4-M9-P20


title: Pool155_Plate2-5-M9-A10


title: Pool155_Plate2-4-M9-P7


title: Pool155_Plate2-4-M9-N8


title: Pool155_Plate2-4-M9-N7


title: Pool155_Plate2-4-M9-N20


title: Pool155_Plate2-4-M9-M7


title: Pool155_Plate2-4-M9-M8


title: Pool155_Plate2-4-M9-N19


title: Pool155_Plate2-4-M9-M20


title: Pool155_Plate2-4-M9-L7


title: Pool155_Plate2-4-M9-L19


title: Pool155_Plate2-4-M9-M19


title: Pool155_Plate2-4-M9-K8


title: Pool155_Plate2-4-M9-L8


title: Pool155_Plate2-4-M9-K7


title: Pool155_Plate2-4-M9-K20


title: Pool155_Plate2-4-M9-K19


title: Pool155_Plate2-4-M9-J19


title: Pool155_Plate2-4-M9-I7


title: Pool155_Plate2-4-M9-I8


title: Pool155_Plate2-4-M9-I20


title: Pool155_Plate2-4-M9-J7


title: Pool155_Plate2-4-M9-J8


title: Pool155_Plate2-4-M9-J20


title: Pool155_Plate2-4-M9-H8


title: Pool155_Plate2-4-M9-G8


title: Pool155_Plate2-4-M9-I19


title: Pool155_Plate2-4-M9-H20


title: Pool155_Plate2-4-M9-H19


title: Pool155_Plate2-4-M9-G7


title: Pool155_Plate2-4-M9-G19


title: Pool155_Plate2-4-M9-G20


title: Pool155_Plate2-4-M9-L20


title: Pool155_Plate2-4-M9-F8


title: Pool155_Plate2-4-M9-F19


title: Pool155_Plate2-4-M9-F20


title: Pool155_Plate2-4-M9-F7


title: Pool155_Plate2-4-M9-E7


title: Pool155_Plate2-4-M9-E20


title: Pool155_Plate2-4-M9-E19


title: Pool155_Plate2-4-M9-D8


title: Pool155_Plate2-4-M9-D7


title: Pool155_Plate2-4-M9-D19


title: Pool155_Plate2-4-M9-E8


title: Pool155_Plate2-4-M9-C20


title: Pool155_Plate2-4-M9-B20


title: Pool155_Plate2-4-M9-C19


title: Pool155_Plate2-4-M9-A20


title: Pool155_Plate2-4-M9-A8


title: Pool155_Plate2-3-M9-P6


title: Pool155_Plate2-4-M9-A7


title: Pool155_Plate2-3-M9-P17


title: Pool155_Plate2-3-M9-P18


title: Pool155_Plate2-3-M9-N5


title: Pool155_Plate2-3-M9-O17


title: Pool155_Plate2-4-M9-C8


title: Pool155_Plate2-3-M9-O6


title: Pool155_Plate2-3-M9-O5


title: Pool155_Plate2-4-M9-B8


title: Pool155_Plate2-3-M9-P5


title: Pool155_Plate2-3-M9-N17


title: Pool155_Plate2-3-M9-N18


title: Pool155_Plate2-3-M9-M5


title: Pool155_Plate2-3-M9-N6


title: Pool155_Plate2-4-M9-B19


title: Pool155_Plate2-4-M9-C7


title: Pool155_Plate2-3-M9-M6


title: Pool155_Plate2-3-M9-L18


title: Pool155_Plate2-3-M9-L6


title: Pool155_Plate2-3-M9-M17


title: Pool155_Plate2-3-M9-K6


title: Pool155_Plate2-3-M9-M18


title: Pool155_Plate2-3-M9-K18


title: Pool155_Plate2-3-M9-J6


title: Pool155_Plate2-3-M9-K5


title: Pool155_Plate2-3-M9-J5


title: Pool155_Plate2-3-M9-J18


title: Pool155_Plate2-3-M9-I18


title: Pool155_Plate2-3-M9-I5


title: Pool155_Plate2-3-M9-I6


title: Pool155_Plate2-3-M9-I17


title: Pool155_Plate2-3-M9-F6


title: Pool155_Plate2-3-M9-H5


title: Pool155_Plate2-3-M9-H18


title: Pool155_Plate2-3-M9-G17


title: Pool155_Plate2-3-M9-G18


title: Pool155_Plate2-3-M9-G6


title: Pool155_Plate2-3-M9-G5


title: Pool155_Plate2-3-M9-H6


title: Pool155_Plate2-3-M9-L17


title: Pool155_Plate2-3-M9-F5


title: Pool155_Plate2-3-M9-F18


title: Pool155_Plate2-3-M9-E6


title: Pool155_Plate2-3-M9-J17


title: Pool155_Plate2-3-M9-E5


title: Pool155_Plate2-3-M9-E17


title: Pool155_Plate2-3-M9-D18


title: Pool155_Plate2-3-M9-D5


title: Pool155_Plate2-3-M9-E18


title: Pool155_Plate2-3-M9-D17


title: Pool155_Plate2-3-M9-C5


title: Pool155_Plate2-3-M9-C17


title: Pool155_Plate2-3-M9-B6


title: Pool155_Plate2-3-M9-C18


title: Pool155_Plate2-3-M9-C6


title: Pool155_Plate2-3-M9-B5


title: Pool155_Plate2-3-M9-B17


title: Pool155_Plate2-3-M9-A18


title: Pool155_Plate2-3-M9-F17


title: Pool155_Plate2-2-M9-P16


title: Pool155_Plate2-2-M9-M3


title: Pool155_Plate2-2-M9-M4


title: Pool155_Plate2-2-M9-L4


title: Pool155_Plate2-2-M9-M16


title: Pool155_Plate2-2-M9-N4


title: Pool155_Plate2-2-M9-O4


title: Pool155_Plate2-2-M9-O16


title: Pool155_Plate2-2-M9-O3


title: Pool155_Plate2-2-M9-L3


title: Pool155_Plate2-2-M9-L16


title: Pool155_Plate2-2-M9-L15


title: Pool155_Plate2-2-M9-K4


title: Pool155_Plate2-2-M9-K3


title: Pool155_Plate2-2-M9-K16


title: Pool155_Plate2-3-M9-A5


title: Pool155_Plate2-2-M9-J3


title: Pool155_Plate2-2-M9-J4


title: Pool155_Plate2-2-M9-K15


title: Pool155_Plate2-2-M9-J16


title: Pool155_Plate2-2-M9-I16


title: Pool155_Plate2-2-M9-I15


title: Pool155_Plate2-2-M9-J15


title: Pool155_Plate2-2-M9-I4


title: Pool155_Plate2-2-M9-N3


title: Pool155_Plate2-2-M9-N16


title: Pool155_Plate2-2-M9-H16


title: Pool155_Plate2-2-M9-G4


title: Pool155_Plate2-2-M9-E4


title: Pool155_Plate2-2-M9-G15


title: Pool155_Plate2-2-M9-G16


title: Pool155_Plate2-2-M9-F15


title: Pool155_Plate2-2-M9-E3


title: Pool155_Plate2-2-M9-F3


title: Pool155_Plate2-2-M9-E16


title: Pool155_Plate2-2-M9-F4


title: Pool155_Plate2-2-M9-E15


title: Pool155_Plate2-2-M9-D4


title: Pool155_Plate2-2-M9-D3


title: Pool155_Plate2-2-M9-D16


title: Pool155_Plate2-2-M9-I3


title: Pool155_Plate2-2-M9-D15


title: Pool155_Plate2-2-M9-C16


title: Pool155_Plate2-2-M9-H4


title: Pool155_Plate2-2-M9-B3


title: Pool155_Plate2-2-M9-B4


title: Pool155_Plate2-2-M9-H3


title: Pool155_Plate2-2-M9-B16


title: Pool155_Plate2-2-M9-A4


title: Pool155_Plate2-2-M9-B15


title: Pool155_Plate2-1-M9-P1


title: Pool155_Plate2-2-M9-A3


title: Pool155_Plate2-1-M9-P14


title: Pool155_Plate2-2-M9-A16


title: Pool155_Plate2-1-M9-P2


title: Pool155_Plate2-2-M9-C15


title: Pool155_Plate2-1-M9-O2


title: Pool155_Plate2-1-M9-O13


title: Pool155_Plate2-1-M9-N1


title: Pool155_Plate2-1-M9-M13


title: Pool155_Plate2-1-M9-N14


title: Pool155_Plate2-1-M9-M1


title: Pool155_Plate2-1-M9-O1


title: Pool155_Plate2-1-M9-K14


title: Pool155_Plate2-2-M9-C4


title: Pool155_Plate2-1-M9-K2


title: Pool155_Plate2-1-M9-L14


title: Pool155_Plate2-1-M9-K1


title: Pool155_Plate2-1-M9-K13


title: Pool155_Plate2-1-M9-J14


title: Pool155_Plate2-1-M9-J2


title: Pool155_Plate2-1-M9-I13


title: Pool155_Plate2-1-M9-J13


title: Pool155_Plate2-1-M9-I2


title: Pool155_Plate2-1-M9-I14


title: Pool155_Plate2-1-M9-H2


title: Pool155_Plate2-1-M9-I1


title: Pool155_Plate2-1-M9-H14


title: Pool155_Plate2-1-M9-M2


title: Pool155_Plate2-1-M9-H13


title: Pool155_Plate2-1-M9-G2


title: Pool155_Plate2-1-M9-G14


title: Pool155_Plate2-1-M9-H1


title: Pool155_Plate2-1-M9-G13


title: Pool155_Plate2-1-M9-N13


title: Pool155_Plate2-1-M9-L2


title: Pool155_Plate2-1-M9-F13


title: Pool155_Plate2-1-M9-F2


title: Pool155_Plate2-1-M9-E2


title: Pool155_Plate2-1-M9-E13


title: Pool155_Plate2-1-M9-E1


title: Pool155_Plate2-1-M9-E14


title: Pool155_Plate2-1-M9-D2


title: Pool155_Plate2-1-M9-C2


title: Pool155_Plate2-1-M9-C14


title: Pool155_Plate2-1-M9-D1


title: Pool155_Plate2-1-M9-C1


title: Pool155_Plate2-1-M9-B2


title: Pool155_Plate2-1-M9-B1


title: Pool155_Plate2-1-M9-B13


title: Pool155_Plate2-1-M9-B14


title: Pool155_Plate2-1-M9-G1


title: Pool155_Plate2-1-M9-A13


title: Pool155_Plate1-6-K9-P24

value: VTA


title: Pool155_Plate2-1-M9-A14


title: Pool155_Plate2-1-M9-A2


title: Pool155_Plate2-1-M9-L1


title: Pool155_Plate2-1-M9-F14


title: Pool155_Plate1-6-K9-P23


title: Pool155_Plate1-6-K9-O24

value: MRN


title: Pool155_Plate2-1-M9-D14


title: Pool155_Plate2-1-M9-F1


title: Pool155_Plate1-6-K9-O23


title: Pool155_Plate1-6-K9-O11


title: Pool155_Plate2-1-M9-D13


title: Pool155_Plate2-1-M9-C13


title: Pool155_Plate1-6-K9-L24


title: Pool155_Plate1-6-K9-M23


title: Pool155_Plate1-6-K9-M11


title: Pool155_Plate1-6-K9-M12


title: Pool155_Plate1-6-K9-L23


title: Pool155_Plate1-6-K9-L12


title: Pool155_Plate1-6-K9-K23


title: Pool155_Plate2-1-M9-A1


title: Pool155_Plate1-6-K9-K24


title: Pool155_Plate1-6-K9-K12


title: Pool155_Plate1-6-K9-J23


title: Pool155_Plate1-6-K9-H23


title: Pool155_Plate1-6-K9-J11


title: Pool155_Plate1-6-K9-H12


title: Pool155_Plate1-6-K9-K11


title: Pool155_Plate1-6-K9-N24


title: Pool155_Plate1-6-K9-G23


title: Pool155_Plate1-6-K9-I23


title: Pool155_Plate1-6-K9-H24


title: Pool155_Plate1-6-K9-G12


title: Pool155_Plate1-6-K9-G11


title: Pool155_Plate1-6-K9-F24


title: Pool155_Plate1-6-K9-F12


title: Pool155_Plate1-6-K9-F11


title: Pool155_Plate1-6-K9-F23


title: Pool155_Plate1-6-K9-E24


title: Pool155_Plate1-6-K9-N23


title: Pool155_Plate1-6-K9-E23


title: Pool155_Plate1-6-K9-L11


title: Pool155_Plate1-6-K9-E12


title: Pool155_Plate1-6-K9-D24


title: Pool155_Plate1-6-K9-D12


title: Pool155_Plate1-6-K9-D23


title: Pool155_Plate1-6-K9-D11


title: Pool155_Plate1-6-K9-E11


title: Pool155_Plate1-6-K9-C23


title: Pool155_Plate1-6-K9-I11


title: Pool155_Plate1-6-K9-B24


title: Pool155_Plate1-6-K9-C12


title: Pool155_Plate1-6-K9-B23


title: Pool155_Plate1-6-K9-C11


title: Pool155_Plate1-6-K9-A24


title: Pool155_Plate1-6-K9-B12


title: Pool155_Plate1-6-K9-A23


title: Pool155_Plate1-5-K9-P21


title: Pool155_Plate1-6-K9-A11


title: Pool155_Plate1-5-K9-O22


title: Pool155_Plate1-6-K9-B11


title: Pool155_Plate1-5-K9-P22


title: Pool155_Plate1-5-K9-O9


title: Pool155_Plate1-6-K9-A12


title: Pool155_Plate1-5-K9-O10


title: Pool155_Plate1-5-K9-O21


title: Pool155_Plate1-5-K9-N22


title: Pool155_Plate1-5-K9-N10


title: Pool155_Plate1-5-K9-N21


title: Pool155_Plate1-5-K9-M9


title: Pool155_Plate1-5-K9-M10


title: Pool155_Plate1-5-K9-M21


title: Pool155_Plate1-6-K9-C24


title: Pool155_Plate1-5-K9-L9


title: Pool155_Plate1-5-K9-L22


title: Pool155_Plate1-5-K9-K21


title: Pool155_Plate1-5-K9-K22


title: Pool155_Plate1-5-K9-K10


title: Pool155_Plate1-5-K9-J9


title: Pool155_Plate1-5-K9-J22


title: Pool155_Plate1-5-K9-J21


title: Pool155_Plate1-5-K9-I9


title: Pool155_Plate1-5-K9-N9


title: Pool155_Plate1-5-K9-H9


title: Pool155_Plate1-5-K9-I10


title: Pool155_Plate1-5-K9-G9


title: Pool155_Plate1-5-K9-G22


title: Pool155_Plate1-5-K9-G21


title: Pool155_Plate1-5-K9-H10


title: Pool155_Plate1-5-K9-F9


title: Pool155_Plate1-5-K9-G10


title: Pool155_Plate1-5-K9-H21


title: Pool155_Plate1-5-K9-L21


title: Pool155_Plate1-5-K9-F21


title: Pool155_Plate1-5-K9-E22


title: Pool155_Plate1-5-K9-F10


title: Pool155_Plate1-5-K9-E9


title: Pool155_Plate1-5-K9-E10


title: Pool155_Plate1-5-K9-D22


title: Pool155_Plate1-5-K9-D10


title: Pool155_Plate1-5-K9-C9


title: Pool155_Plate1-5-K9-D9


title: Pool155_Plate1-5-K9-C21


title: Pool155_Plate1-5-K9-C10


title: Pool155_Plate1-5-K9-I22


title: Pool155_Plate1-5-K9-B22


title: Pool155_Plate1-5-K9-B9


title: Pool155_Plate1-5-K9-H22


title: Pool155_Plate1-5-K9-B21


title: Pool155_Plate1-5-K9-A9


title: Pool155_Plate1-5-K9-B10


title: Pool155_Plate1-5-K9-A22


title: Pool155_Plate1-5-K9-A10


title: Pool155_Plate1-5-K9-A21


title: Pool155_Plate1-4-K9-P7


title: Pool155_Plate1-4-K9-P20


title: Pool155_Plate1-4-K9-P19


title: Pool155_Plate1-4-K9-O8


title: Pool155_Plate1-4-K9-O7


title: Pool155_Plate1-4-K9-N8


title: Pool155_Plate1-4-K9-O19


title: Pool155_Plate1-4-K9-N7


title: Pool155_Plate1-4-K9-N19


title: Pool155_Plate1-4-K9-M8


title: Pool155_Plate1-4-K9-M7


title: Pool155_Plate1-4-K9-K8


title: Pool155_Plate1-4-K9-L20


title: Pool155_Plate1-4-K9-L8


title: Pool155_Plate1-4-K9-L7


title: Pool155_Plate1-4-K9-M20


title: Pool155_Plate1-4-K9-K7


title: Pool155_Plate1-4-K9-M19


title: Pool155_Plate1-4-K9-J7


title: Pool155_Plate1-4-K9-K19


title: Pool155_Plate1-4-K9-K20


title: Pool155_Plate1-4-K9-J8


title: Pool155_Plate1-4-K9-J19


title: Pool155_Plate1-4-K9-I7


title: Pool155_Plate1-4-K9-H8


title: Pool155_Plate1-4-K9-I20


title: Pool155_Plate1-4-K9-H7


title: Pool155_Plate1-4-K9-O20


title: Pool155_Plate1-4-K9-J20


title: Pool155_Plate1-4-K9-G7


title: Pool155_Plate1-4-K9-G8


title: Pool155_Plate1-4-K9-H20


title: Pool155_Plate1-4-K9-G20


title: Pool155_Plate1-4-K9-G19


title: Pool155_Plate1-4-K9-F7


title: Pool155_Plate1-4-K9-F8


title: Pool155_Plate1-4-K9-F20


title: Pool155_Plate1-4-K9-F19


title: Pool155_Plate1-4-K9-E7


title: Pool155_Plate1-4-K9-E20


title: Pool155_Plate1-4-K9-E8


title: Pool155_Plate1-4-K9-D20


title: Pool155_Plate1-4-K9-D7


title: Pool155_Plate1-4-K9-E19


title: Pool155_Plate1-4-K9-C8


title: Pool155_Plate1-4-K9-I19


title: Pool155_Plate1-4-K9-C19


title: Pool155_Plate1-4-K9-A8


title: Pool155_Plate1-4-K9-B20


title: Pool155_Plate1-4-K9-A7


title: Pool155_Plate1-3-K9-P5


title: Pool155_Plate1-4-K9-A20


title: Pool155_Plate1-3-K9-P6


title: Pool155_Plate1-4-K9-C7


title: Pool155_Plate1-3-K9-O6


title: Pool155_Plate1-3-K9-P18


title: Pool155_Plate1-3-K9-O18


title: Pool155_Plate1-4-K9-A19


title: Pool155_Plate1-3-K9-N5


title: Pool155_Plate1-3-K9-N18


title: Pool155_Plate1-3-K9-M6


title: Pool155_Plate1-3-K9-N6


title: Pool155_Plate1-3-K9-O17


title: Pool155_Plate1-3-K9-N17


title: Pool155_Plate1-3-K9-M5


title: Pool155_Plate1-3-K9-M18


title: Pool155_Plate1-4-K9-D19


title: Pool155_Plate1-4-K9-D8


title: Pool155_Plate1-3-K9-L17


title: Pool155_Plate1-3-K9-L5


title: Pool155_Plate1-3-K9-K5


title: Pool155_Plate1-3-K9-K6


title: Pool155_Plate1-3-K9-K17


title: Pool155_Plate1-3-K9-J5


title: Pool155_Plate1-3-K9-J18


title: Pool155_Plate1-3-K9-J17


title: Pool155_Plate1-3-K9-J6


title: Pool155_Plate1-3-K9-I6


title: Pool155_Plate1-3-K9-I5


title: Pool155_Plate1-3-K9-I17


title: Pool155_Plate1-3-K9-H18


title: Pool155_Plate1-3-K9-H17


title: Pool155_Plate1-3-K9-H5


title: Pool155_Plate1-3-K9-G6


title: Pool155_Plate1-3-K9-H6


title: Pool155_Plate1-3-K9-G5


title: Pool155_Plate1-3-K9-G18


title: Pool155_Plate1-3-K9-F17


title: Pool155_Plate1-3-K9-F5


title: Pool155_Plate1-3-K9-E6


title: Pool155_Plate1-3-K9-F18


title: Pool155_Plate1-3-K9-G17


title: Pool155_Plate1-3-K9-E5


title: Pool155_Plate1-3-K9-E18


title: Pool155_Plate1-3-K9-E17


title: Pool155_Plate1-3-K9-D6


title: Pool155_Plate1-3-K9-C5


title: Pool155_Plate1-3-K9-D18


title: Pool155_Plate1-3-K9-D17


title: Pool155_Plate1-3-K9-D5


title: Pool155_Plate1-3-K9-C6


title: Pool155_Plate1-3-K9-I18


title: Pool155_Plate1-3-K9-C18


title: Pool155_Plate1-3-K9-C17


title: Pool155_Plate1-3-K9-B5


title: Pool155_Plate1-3-K9-B6


title: Pool155_Plate1-3-K9-A6


title: Pool155_Plate1-3-K9-A18


title: Pool155_Plate1-3-K9-A5


title: Pool155_Plate1-3-K9-A17


title: Pool155_Plate1-2-K9-P4


title: Pool155_Plate1-3-K9-B17


title: Pool155_Plate1-2-K9-O4


title: Pool155_Plate1-2-K9-O3


title: Pool155_Plate1-2-K9-P15


title: Pool155_Plate1-2-K9-N4


title: Pool155_Plate1-2-K9-N3


title: Pool155_Plate1-2-K9-M15


title: Pool155_Plate1-2-K9-M16


title: Pool155_Plate1-2-K9-L4


title: Pool155_Plate1-2-K9-L16


title: Pool155_Plate1-2-K9-L3


title: Pool155_Plate1-2-K9-L15


title: Pool155_Plate1-2-K9-K3


title: Pool155_Plate1-2-K9-K15


title: Pool155_Plate1-2-K9-J4


title: Pool155_Plate1-2-K9-K16


title: Pool155_Plate1-2-K9-J3


title: Pool155_Plate1-2-K9-J16


title: Pool155_Plate1-2-K9-J15


title: Pool155_Plate1-2-K9-I4


title: Pool155_Plate1-2-K9-O16


title: Pool155_Plate1-2-K9-N15


title: Pool155_Plate1-2-K9-M3


title: Pool155_Plate1-2-K9-M4


title: Pool155_Plate1-2-K9-K4


title: Pool155_Plate1-2-K9-E3


title: Pool155_Plate1-2-K9-E16


title: Pool155_Plate1-2-K9-H3


title: Pool155_Plate1-2-K9-G16


title: Pool155_Plate1-2-K9-E4


title: Pool155_Plate1-2-K9-F3


title: Pool155_Plate1-2-K9-E15


title: Pool155_Plate1-2-K9-D16


title: Pool155_Plate1-2-K9-F4


title: Pool155_Plate1-2-K9-D15


title: Pool155_Plate1-2-K9-F15


title: Pool155_Plate1-2-K9-D4


title: Pool155_Plate1-2-K9-H15


title: Pool155_Plate1-2-K9-I15


title: Pool155_Plate1-2-K9-I16


title: Pool155_Plate1-2-K9-H16


title: Pool155_Plate1-2-K9-D3


title: Pool155_Plate1-2-K9-G4


title: Pool155_Plate1-2-K9-F16


title: Pool155_Plate1-2-K9-H4


title: Pool155_Plate1-2-K9-C4


title: Pool155_Plate1-2-K9-I3


title: Pool155_Plate1-2-K9-G3


title: Pool155_Plate1-2-K9-C15


title: Pool155_Plate1-2-K9-C16


title: Pool155_Plate1-2-K9-B4


title: Pool155_Plate1-2-K9-B16


title: Pool155_Plate1-2-K9-B3


title: Pool155_Plate1-2-K9-C3


title: Pool155_Plate1-1-K9-P1


title: Pool155_Plate1-1-K9-O2


title: Pool155_Plate1-2-K9-B15


title: Pool155_Plate1-1-K9-P2


title: Pool155_Plate1-1-K9-O13


title: Pool155_Plate1-2-K9-A3


title: Pool155_Plate1-1-K9-O14


title: Pool155_Plate1-2-K9-A16


title: Pool155_Plate1-2-K9-A15


title: Pool155_Plate1-2-K9-A4


title: Pool155_Plate1-1-K9-K14


title: Pool155_Plate1-1-K9-K2


title: Pool155_Plate1-1-K9-J2


title: Pool155_Plate1-1-K9-L1


title: Pool155_Plate1-1-K9-K13


title: Pool155_Plate1-1-K9-K1


title: Pool155_Plate1-1-K9-N2


title: Pool155_Plate1-1-K9-M13


title: Pool155_Plate1-1-K9-N13


title: Pool155_Plate1-1-K9-L2


title: Pool155_Plate1-1-K9-O1


title: Pool155_Plate1-1-K9-M2


title: Pool155_Plate1-1-K9-M14


title: Pool155_Plate1-1-K9-N1


title: Pool155_Plate1-1-K9-J14


title: Pool155_Plate1-1-K9-J13


title: Pool155_Plate1-1-K9-I14


title: Pool155_Plate1-1-K9-I13


title: Pool155_Plate1-1-K9-H13


title: Pool155_Plate1-1-K9-G2


title: Pool155_Plate1-1-K9-I2


title: Pool155_Plate1-1-K9-J1


title: Pool155_Plate1-1-K9-G13


title: Pool155_Plate1-1-K9-G14


title: Pool155_Plate1-1-K9-G1


title: Pool155_Plate1-1-K9-H14


title: Pool155_Plate1-1-K9-F2


title: Pool155_Plate1-1-K9-E2


title: Pool155_Plate1-1-K9-E13


title: Pool155_Plate1-1-K9-F13


title: Pool155_Plate1-1-K9-F14


title: Pool155_Plate1-1-K9-E14


title: Pool155_Plate1-1-K9-F1


title: Pool155_Plate1-1-K9-H1


title: Pool155_Plate1-1-K9-C2


title: Pool155_Plate1-1-K9-D1


title: Pool155_Plate1-1-K9-D2


title: Pool155_Plate1-1-K9-I1


title: Pool155_Plate1-1-K9-E1


title: Pool155_Plate1-1-K9-C14


title: Pool155_Plate1-1-K9-D13


title: Pool155_Plate1-1-K9-H2


title: Pool155_Plate1-1-K9-C13


title: Pool155_Plate1-1-K9-B14


title: Pool155_Plate1-1-K9-B13


title: Pool155_Plate1-1-K9-B2


title: Pool154_Plate8-6-A3-P11

value: HY


title: Pool154_Plate8-6-A3-P23


title: Pool154_Plate8-6-A3-P12


title: Pool155_Plate1-1-K9-B1


title: Pool155_Plate1-1-K9-A13


title: Pool154_Plate8-6-A3-O23


title: Pool155_Plate1-1-K9-D14


title: Pool155_Plate1-1-K9-C1


title: Pool154_Plate8-6-A3-N24


title: Pool154_Plate8-6-A3-N12


title: Pool154_Plate8-6-A3-M12


title: Pool154_Plate8-6-A3-L23


title: Pool154_Plate8-6-A3-L12


title: Pool154_Plate8-6-A3-J23


title: Pool154_Plate8-6-A3-M24


title: Pool154_Plate8-6-A3-J12


title: Pool154_Plate8-6-A3-J11


title: Pool154_Plate8-6-A3-H23


title: Pool154_Plate8-6-A3-I11


title: Pool155_Plate1-1-K9-A2


title: Pool154_Plate8-6-A3-M23


title: Pool154_Plate8-6-A3-I24


title: Pool154_Plate8-6-A3-H12


title: Pool154_Plate8-6-A3-O24


title: Pool154_Plate8-6-A3-H11


title: Pool154_Plate8-6-A3-E24


title: Pool154_Plate8-6-A3-F12


title: Pool154_Plate8-6-A3-E12


title: Pool154_Plate8-6-A3-F23


title: Pool154_Plate8-6-A3-F11


title: Pool154_Plate8-6-A3-G24


title: Pool154_Plate8-6-A3-G23


title: Pool154_Plate8-6-A3-E23


title: Pool154_Plate8-6-A3-D23


title: Pool154_Plate8-6-A3-M11


title: Pool154_Plate8-6-A3-D12


title: Pool154_Plate8-6-A3-L11


title: Pool154_Plate8-6-A3-C24


title: Pool154_Plate8-6-A3-B23


title: Pool154_Plate8-6-A3-C23


title: Pool154_Plate8-5-A3-P22


title: Pool154_Plate8-6-A3-B11


title: Pool154_Plate8-5-A3-N10


title: Pool154_Plate8-6-A3-B12


title: Pool154_Plate8-6-A3-A24


title: Pool154_Plate8-5-A3-O9


title: Pool154_Plate8-5-A3-O21


title: Pool154_Plate8-5-A3-N22


title: Pool154_Plate8-5-A3-P10


title: Pool154_Plate8-5-A3-K22


title: Pool154_Plate8-5-A3-L10


title: Pool154_Plate8-5-A3-M21


title: Pool154_Plate8-5-A3-L22


title: Pool154_Plate8-5-A3-J22


title: Pool154_Plate8-5-A3-K21


title: Pool154_Plate8-5-A3-J21


title: Pool154_Plate8-5-A3-I21


title: Pool154_Plate8-5-A3-I22


title: Pool154_Plate8-5-A3-J10


title: Pool154_Plate8-5-A3-H22


title: Pool154_Plate8-5-A3-H9


title: Pool154_Plate8-5-A3-H10


title: Pool154_Plate8-5-A3-H21


title: Pool154_Plate8-5-A3-P9


title: Pool154_Plate8-5-A3-G22


title: Pool154_Plate8-6-A3-A12


title: Pool154_Plate8-5-A3-F9


title: Pool154_Plate8-5-A3-G21


title: Pool154_Plate8-5-A3-F22


title: Pool154_Plate8-5-A3-E9


title: Pool154_Plate8-5-A3-F21


title: Pool154_Plate8-5-A3-F10


title: Pool154_Plate8-5-A3-E22


title: Pool154_Plate8-5-A3-E21


title: Pool154_Plate8-5-A3-E10


title: Pool154_Plate8-5-A3-D21


title: Pool154_Plate8-5-A3-D9


title: Pool154_Plate8-5-A3-C9


title: Pool154_Plate8-5-A3-D10


title: Pool154_Plate8-5-A3-B9


title: Pool154_Plate8-5-A3-C22


title: Pool154_Plate8-5-A3-B22


title: Pool154_Plate8-5-A3-B10


title: Pool154_Plate8-5-A3-A22


title: Pool154_Plate8-4-A3-P8


title: Pool154_Plate8-5-A3-A9


title: Pool154_Plate8-4-A3-P20


title: Pool154_Plate8-4-A3-O8


title: Pool154_Plate8-4-A3-N8


title: Pool154_Plate8-4-A3-O7


title: Pool154_Plate8-4-A3-N20


title: Pool154_Plate8-4-A3-N7


title: Pool154_Plate8-4-A3-M8


title: Pool154_Plate8-4-A3-M7


title: Pool154_Plate8-5-A3-D22


title: Pool154_Plate8-4-A3-L8


title: Pool154_Plate8-4-A3-K7


title: Pool154_Plate8-4-A3-L7


title: Pool154_Plate8-5-A3-C21


title: Pool154_Plate8-4-A3-K20


title: Pool154_Plate8-5-A3-B21


title: Pool154_Plate8-4-A3-J8


title: Pool154_Plate8-4-A3-J7


title: Pool154_Plate8-4-A3-J20


title: Pool154_Plate8-4-A3-H20


title: Pool154_Plate8-4-A3-H7


title: Pool154_Plate8-4-A3-I7


title: Pool154_Plate8-4-A3-H8


title: Pool154_Plate8-4-A3-P19


title: Pool154_Plate8-4-A3-G7


title: Pool154_Plate8-4-A3-J19


title: Pool154_Plate8-4-A3-I20


title: Pool154_Plate8-4-A3-G19


title: Pool154_Plate8-4-A3-G8


title: Pool154_Plate8-4-A3-F20


title: Pool154_Plate8-4-A3-G20


title: Pool154_Plate8-4-A3-E20


title: Pool154_Plate8-4-A3-F19


title: Pool154_Plate8-4-A3-E8


title: Pool154_Plate8-4-A3-F7


title: Pool154_Plate8-4-A3-C8


title: Pool154_Plate8-4-A3-C7


title: Pool154_Plate8-4-A3-D19


title: Pool154_Plate8-4-A3-D8


title: Pool154_Plate8-4-A3-C20


title: Pool154_Plate8-4-A3-E19


title: Pool154_Plate8-4-A3-B20


title: Pool154_Plate8-4-A3-C19


title: Pool154_Plate8-4-A3-B19


title: Pool154_Plate8-4-A3-A8


title: Pool154_Plate8-4-A3-A7


title: Pool154_Plate8-4-A3-A19


title: Pool154_Plate8-3-A3-P6


title: Pool154_Plate8-4-A3-A20


title: Pool154_Plate8-3-A3-P17


title: Pool154_Plate8-3-A3-P18


title: Pool154_Plate8-3-A3-P5


title: Pool154_Plate8-3-A3-O6


title: Pool154_Plate8-3-A3-N5


title: Pool154_Plate8-3-A3-N18


title: Pool154_Plate8-3-A3-O5


title: Pool154_Plate8-3-A3-L17


title: Pool154_Plate8-3-A3-L18


title: Pool154_Plate8-3-A3-K6


title: Pool154_Plate8-3-A3-K18


title: Pool154_Plate8-3-A3-K5


title: Pool154_Plate8-3-A3-L5


title: Pool154_Plate8-3-A3-J5


title: Pool154_Plate8-3-A3-J17


title: Pool154_Plate8-3-A3-I6


title: Pool154_Plate8-3-A3-J18


title: Pool154_Plate8-3-A3-I18


title: Pool154_Plate8-3-A3-I17


title: Pool154_Plate8-3-A3-H6


title: Pool154_Plate8-3-A3-H18


title: Pool154_Plate8-3-A3-G6


title: Pool154_Plate8-3-A3-M6


title: Pool154_Plate8-3-A3-M5


title: Pool154_Plate8-3-A3-G18


title: Pool154_Plate8-3-A3-G5


title: Pool154_Plate8-3-A3-M17


title: Pool154_Plate8-3-A3-L6


title: Pool154_Plate8-3-A3-G17


title: Pool154_Plate8-3-A3-F6


title: Pool154_Plate8-3-A3-H17


title: Pool154_Plate8-3-A3-F17


title: Pool154_Plate8-3-A3-F18


title: Pool154_Plate8-3-A3-F5


title: Pool154_Plate8-3-A3-E6


title: Pool154_Plate8-3-A3-E18


title: Pool154_Plate8-3-A3-D6


title: Pool154_Plate8-3-A3-D17


title: Pool154_Plate8-3-A3-D18


title: Pool154_Plate8-3-A3-D5


title: Pool154_Plate8-3-A3-E5


title: Pool154_Plate8-3-A3-C17


title: Pool154_Plate8-3-A3-B5


title: Pool154_Plate8-3-A3-B17


title: Pool154_Plate8-3-A3-C6


title: Pool154_Plate8-3-A3-B18


title: Pool154_Plate8-3-A3-C5


title: Pool154_Plate8-2-A3-P16


title: Pool154_Plate8-3-A3-A6


title: Pool154_Plate8-3-A3-A5


title: Pool154_Plate8-2-A3-P4


title: Pool154_Plate8-2-A3-P15


title: Pool154_Plate8-2-A3-O4


title: Pool154_Plate8-2-A3-O3


title: Pool154_Plate8-2-A3-N4


title: Pool154_Plate8-2-A3-M4


title: Pool154_Plate8-2-A3-N15


title: Pool154_Plate8-2-A3-N3


title: Pool154_Plate8-2-A3-M3


title: Pool154_Plate8-2-A3-J4


title: Pool154_Plate8-2-A3-K4


title: Pool154_Plate8-2-A3-L15


title: Pool154_Plate8-2-A3-J16


title: Pool154_Plate8-2-A3-J15


title: Pool154_Plate8-2-A3-I3


title: Pool154_Plate8-2-A3-I16


title: Pool154_Plate8-2-A3-H4


title: Pool154_Plate8-2-A3-G3


title: Pool154_Plate8-2-A3-H16


title: Pool154_Plate8-2-A3-I15


title: Pool154_Plate8-2-A3-G15


title: Pool154_Plate8-2-A3-F16


title: Pool154_Plate8-2-A3-F4


title: Pool154_Plate8-2-A3-G16


title: Pool154_Plate8-2-A3-E4


title: Pool154_Plate8-2-A3-F3


title: Pool154_Plate8-2-A3-D4


title: Pool154_Plate8-2-A3-E3


title: Pool154_Plate8-2-A3-D16


title: Pool154_Plate8-2-A3-D3


title: Pool154_Plate8-2-A3-D15


title: Pool154_Plate8-2-A3-M15


title: Pool154_Plate8-2-A3-C3


title: Pool154_Plate8-2-A3-B4


title: Pool154_Plate8-2-A3-B3


title: Pool154_Plate8-2-A3-B16


title: Pool154_Plate8-2-A3-B15


title: Pool154_Plate8-2-A3-A16


title: Pool154_Plate8-2-A3-A15


title: Pool154_Plate8-1-A3-P2


title: Pool154_Plate8-2-A3-A3


title: Pool154_Plate8-1-A3-P14


title: Pool154_Plate8-1-A3-P13


title: Pool154_Plate8-1-A3-N2


title: Pool154_Plate8-1-A3-N14


title: Pool154_Plate8-1-A3-L14


title: Pool154_Plate8-1-A3-J14


title: Pool154_Plate8-1-A3-N1


title: Pool154_Plate8-1-A3-N13


title: Pool154_Plate8-1-A3-K13


title: Pool154_Plate8-1-A3-L13


title: Pool154_Plate8-1-A3-I1


title: Pool154_Plate8-1-A3-I2


title: Pool154_Plate8-1-A3-I13


title: Pool154_Plate8-1-A3-H14


title: Pool154_Plate8-1-A3-H13


title: Pool154_Plate8-1-A3-H1


title: Pool154_Plate8-1-A3-O2


title: Pool154_Plate8-1-A3-G2


title: Pool154_Plate8-1-A3-G14


title: Pool154_Plate8-1-A3-M2


title: Pool154_Plate8-1-A3-F13


title: Pool154_Plate8-1-A3-F14


title: Pool154_Plate8-1-A3-F1


title: Pool154_Plate8-1-A3-E2


title: Pool154_Plate8-1-A3-E1


title: Pool154_Plate8-1-A3-F2


title: Pool154_Plate8-1-A3-B13


title: Pool154_Plate8-1-A3-H2


title: Pool154_Plate8-1-A3-J13


title: Pool154_Plate8-1-A3-A1


title: Pool154_Plate8-1-A3-B14


title: Pool154_Plate8-1-A3-B2


title: Pool154_Plate8-1-A3-J2


title: Pool154_Plate8-1-A3-J1


title: Pool154_Plate7-6-O1-P24

value: MY

value: CBX


title: Pool154_Plate7-6-O1-P12


title: Pool154_Plate7-6-O1-P23


title: Pool154_Plate7-6-O1-O23


title: Pool154_Plate7-6-O1-O11


title: Pool154_Plate7-6-O1-N24


title: Pool154_Plate7-6-O1-N12


title: Pool154_Plate7-6-O1-N23


title: Pool154_Plate7-6-O1-N11


title: Pool154_Plate7-6-O1-M23


title: Pool154_Plate7-6-O1-M24


title: Pool154_Plate7-6-O1-M12


title: Pool154_Plate7-6-O1-M11


title: Pool154_Plate8-1-A3-D14


title: Pool154_Plate7-6-O1-K23


title: Pool154_Plate7-6-O1-K24


title: Pool154_Plate7-6-O1-P11


title: Pool154_Plate7-6-O1-L11


title: Pool154_Plate7-6-O1-O12


title: Pool154_Plate7-6-O1-J23


title: Pool154_Plate7-6-O1-J24


title: Pool154_Plate7-6-O1-K11


title: Pool154_Plate7-6-O1-J12


title: Pool154_Plate7-6-O1-J11


title: Pool154_Plate7-6-O1-I23


title: Pool154_Plate7-6-O1-I24


title: Pool154_Plate7-6-O1-H23


title: Pool154_Plate7-6-O1-I11


title: Pool154_Plate7-6-O1-I12


title: Pool154_Plate7-6-O1-H11


title: Pool154_Plate7-6-O1-H12


title: Pool154_Plate7-6-O1-G23


title: Pool154_Plate7-6-O1-E24


title: Pool154_Plate7-6-O1-F11


title: Pool154_Plate7-6-O1-F12


title: Pool154_Plate7-6-O1-G12


title: Pool154_Plate7-6-O1-G11


title: Pool154_Plate7-6-O1-L12


title: Pool154_Plate7-6-O1-E23


title: Pool154_Plate7-6-O1-K12


title: Pool154_Plate7-6-O1-E12


title: Pool154_Plate7-6-O1-D24


title: Pool154_Plate7-6-O1-C12


title: Pool154_Plate7-6-O1-D11


title: Pool154_Plate7-6-O1-C23


title: Pool154_Plate7-6-O1-B24


title: Pool154_Plate7-6-O1-D23


title: Pool154_Plate7-6-O1-A24


title: Pool154_Plate7-6-O1-B12


title: Pool154_Plate7-6-O1-A12


title: Pool154_Plate7-6-O1-B23


title: Pool154_Plate7-6-O1-B11


title: Pool154_Plate7-5-O1-P9


title: Pool154_Plate7-5-O1-P22


title: Pool154_Plate7-5-O1-O9


title: Pool154_Plate7-5-O1-O22


title: Pool154_Plate7-5-O1-P10


title: Pool154_Plate7-5-O1-P21


title: Pool154_Plate7-6-O1-D12


title: Pool154_Plate7-5-O1-M9


title: Pool154_Plate7-5-O1-N10


title: Pool154_Plate7-5-O1-N21


title: Pool154_Plate7-5-O1-M22


title: Pool154_Plate7-5-O1-L9


title: Pool154_Plate7-5-O1-N22


title: Pool154_Plate7-5-O1-M21


title: Pool154_Plate7-5-O1-L22


title: Pool154_Plate7-5-O1-L10


title: Pool154_Plate7-6-O1-E11


title: Pool154_Plate7-5-O1-K9


title: Pool154_Plate7-5-O1-K10


title: Pool154_Plate7-5-O1-K22


title: Pool154_Plate7-6-O1-A11


title: Pool154_Plate7-5-O1-J22


title: Pool154_Plate7-5-O1-J21


title: Pool154_Plate7-5-O1-J9


title: Pool154_Plate7-5-O1-I22


title: Pool154_Plate7-5-O1-I10


title: Pool154_Plate7-5-O1-N9


title: Pool154_Plate7-5-O1-H22


title: Pool154_Plate7-5-O1-H9


title: Pool154_Plate7-5-O1-G9


title: Pool154_Plate7-5-O1-G22


title: Pool154_Plate7-5-O1-H10


title: Pool154_Plate7-5-O1-F9


title: Pool154_Plate7-5-O1-E9


title: Pool154_Plate7-5-O1-F10


title: Pool154_Plate7-5-O1-F21


title: Pool154_Plate7-5-O1-F22


title: Pool154_Plate7-5-O1-E22


title: Pool154_Plate7-5-O1-J10


title: Pool154_Plate7-5-O1-E21


title: Pool154_Plate7-5-O1-D22


title: Pool154_Plate7-5-O1-I9


title: Pool154_Plate7-5-O1-C9


title: Pool154_Plate7-5-O1-D9


title: Pool154_Plate7-5-O1-D10


title: Pool154_Plate7-5-O1-D21


title: Pool154_Plate7-5-O1-K21


title: Pool154_Plate7-5-O1-E10


title: Pool154_Plate7-5-O1-C10


title: Pool154_Plate7-5-O1-C21


title: Pool154_Plate7-5-O1-B9


title: Pool154_Plate7-5-O1-C22


title: Pool154_Plate7-5-O1-H21


title: Pool154_Plate7-5-O1-B21


title: Pool154_Plate7-5-O1-B22


title: Pool154_Plate7-5-O1-B10


title: Pool154_Plate7-5-O1-A9


title: Pool154_Plate7-5-O1-A21


title: Pool154_Plate7-5-O1-A10


title: Pool154_Plate7-4-O1-P7


title: Pool154_Plate7-4-O1-P8


title: Pool154_Plate7-4-O1-O20


title: Pool154_Plate7-4-O1-O7


title: Pool154_Plate7-4-O1-O19


title: Pool154_Plate7-4-O1-P19


title: Pool154_Plate7-4-O1-N7


title: Pool154_Plate7-4-O1-N20


title: Pool154_Plate7-4-O1-M8


title: Pool154_Plate7-4-O1-M7


title: Pool154_Plate7-4-O1-N19


title: Pool154_Plate7-4-O1-J8


title: Pool154_Plate7-4-O1-J19


title: Pool154_Plate7-4-O1-I8


title: Pool154_Plate7-4-O1-I19


title: Pool154_Plate7-4-O1-I20


title: Pool154_Plate7-4-O1-J7


title: Pool154_Plate7-4-O1-J20


title: Pool154_Plate7-4-O1-P20


title: Pool154_Plate7-4-O1-L20


title: Pool154_Plate7-4-O1-M19


title: Pool154_Plate7-4-O1-L7


title: Pool154_Plate7-4-O1-L8


title: Pool154_Plate7-4-O1-K7


title: Pool154_Plate7-4-O1-L19


title: Pool154_Plate7-4-O1-G8


title: Pool154_Plate7-4-O1-H7


title: Pool154_Plate7-4-O1-G19


title: Pool154_Plate7-4-O1-K19


title: Pool154_Plate7-4-O1-M20


title: Pool154_Plate7-4-O1-K8


title: Pool154_Plate7-4-O1-K20


title: Pool154_Plate7-4-O1-N8


title: Pool154_Plate7-4-O1-H20


title: Pool154_Plate7-4-O1-H19


title: Pool154_Plate7-4-O1-E20


title: Pool154_Plate7-4-O1-E7


title: Pool154_Plate7-4-O1-F8


title: Pool154_Plate7-4-O1-F20


title: Pool154_Plate7-4-O1-D19


title: Pool154_Plate7-4-O1-E19


title: Pool154_Plate7-4-O1-F7


title: Pool154_Plate7-4-O1-C8


title: Pool154_Plate7-4-O1-D7


title: Pool154_Plate7-4-O1-B20


title: Pool154_Plate7-4-O1-B7


title: Pool154_Plate7-4-O1-B8


title: Pool154_Plate7-4-O1-A7


title: Pool154_Plate7-4-O1-A8


title: Pool154_Plate7-4-O1-B19


title: Pool154_Plate7-3-O1-O6


title: Pool154_Plate7-3-O1-P5


title: Pool154_Plate7-3-O1-P18


title: Pool154_Plate7-3-O1-P17


title: Pool154_Plate7-4-O1-A20


title: Pool154_Plate7-4-O1-A19


title: Pool154_Plate7-3-O1-O5


title: Pool154_Plate7-3-O1-P6


title: Pool154_Plate7-3-O1-N6


title: Pool154_Plate7-4-O1-C19


title: Pool154_Plate7-3-O1-M6


title: Pool154_Plate7-3-O1-N5


title: Pool154_Plate7-3-O1-N17


title: Pool154_Plate7-3-O1-N18


title: Pool154_Plate7-3-O1-M17


title: Pool154_Plate7-3-O1-M18


title: Pool154_Plate7-3-O1-L6


title: Pool154_Plate7-3-O1-L5


title: Pool154_Plate7-3-O1-M5


title: Pool154_Plate7-3-O1-L18


title: Pool154_Plate7-3-O1-L17


title: Pool154_Plate7-3-O1-H5


title: Pool154_Plate7-3-O1-J18


title: Pool154_Plate7-3-O1-G18


title: Pool154_Plate7-3-O1-K6


title: Pool154_Plate7-3-O1-J17


title: Pool154_Plate7-3-O1-I17


title: Pool154_Plate7-3-O1-K17


title: Pool154_Plate7-3-O1-K5


title: Pool154_Plate7-3-O1-J6


title: Pool154_Plate7-3-O1-I5


title: Pool154_Plate7-3-O1-H6


title: Pool154_Plate7-3-O1-G5


title: Pool154_Plate7-3-O1-I6


title: Pool154_Plate7-3-O1-J5


title: Pool154_Plate7-3-O1-G6


title: Pool154_Plate7-3-O1-K18


title: Pool154_Plate7-3-O1-I18


title: Pool154_Plate7-3-O1-F6


title: Pool154_Plate7-3-O1-F18


title: Pool154_Plate7-3-O1-F5


title: Pool154_Plate7-3-O1-E18


title: Pool154_Plate7-3-O1-F17


title: Pool154_Plate7-3-O1-D18


title: Pool154_Plate7-3-O1-D17


title: Pool154_Plate7-3-O1-E17


title: Pool154_Plate7-3-O1-E6


title: Pool154_Plate7-3-O1-D6


title: Pool154_Plate7-3-O1-C18


title: Pool154_Plate7-3-O1-A5


title: Pool154_Plate7-3-O1-B17


title: Pool154_Plate7-3-O1-C17


title: Pool154_Plate7-2-O1-P15


title: Pool154_Plate7-2-O1-O15


title: Pool154_Plate7-2-O1-P4


title: Pool154_Plate7-3-O1-C5


title: Pool154_Plate7-2-O1-O16


title: Pool154_Plate7-3-O1-B5


title: Pool154_Plate7-3-O1-A18


title: Pool154_Plate7-2-O1-N4


title: Pool154_Plate7-2-O1-O3


title: Pool154_Plate7-2-O1-P3


title: Pool154_Plate7-3-O1-D5


title: Pool154_Plate7-3-O1-B18


title: Pool154_Plate7-2-O1-N3


title: Pool154_Plate7-2-O1-N15


title: Pool154_Plate7-2-O1-M4


title: Pool154_Plate7-2-O1-M16


title: Pool154_Plate7-2-O1-M3


title: Pool154_Plate7-3-O1-E5


title: Pool154_Plate7-2-O1-L4


title: Pool154_Plate7-2-O1-M15


title: Pool154_Plate7-2-O1-N16


title: Pool154_Plate7-2-O1-L3


title: Pool154_Plate7-3-O1-A6


title: Pool154_Plate7-2-O1-P16


title: Pool154_Plate7-2-O1-L16


title: Pool154_Plate7-2-O1-K4


title: Pool154_Plate7-2-O1-K3


title: Pool154_Plate7-2-O1-L15


title: Pool154_Plate7-2-O1-K16


title: Pool154_Plate7-2-O1-I3


title: Pool154_Plate7-2-O1-K15


title: Pool154_Plate7-2-O1-J15


title: Pool154_Plate7-2-O1-I16


title: Pool154_Plate7-2-O1-J16


title: Pool154_Plate7-2-O1-H15


title: Pool154_Plate7-2-O1-H4


title: Pool154_Plate7-2-O1-J3


title: Pool154_Plate7-2-O1-H3


title: Pool154_Plate7-2-O1-H16


title: Pool154_Plate7-2-O1-G4


title: Pool154_Plate7-2-O1-G3


title: Pool154_Plate7-2-O1-G16


title: Pool154_Plate7-2-O1-F4


title: Pool154_Plate7-2-O1-J4


title: Pool154_Plate7-2-O1-F16


title: Pool154_Plate7-2-O1-F3


title: Pool154_Plate7-2-O1-E16


title: Pool154_Plate7-2-O1-F15


title: Pool154_Plate7-2-O1-E4


title: Pool154_Plate7-2-O1-E15


title: Pool154_Plate7-2-O1-I4


title: Pool154_Plate7-2-O1-I15


title: Pool154_Plate7-2-O1-D4


title: Pool154_Plate7-2-O1-C4


title: Pool154_Plate7-2-O1-D3


title: Pool154_Plate7-2-O1-D15


title: Pool154_Plate7-2-O1-C3


title: Pool154_Plate7-2-O1-C16


title: Pool154_Plate7-2-O1-C15


title: Pool154_Plate7-1-O1-P14


title: Pool154_Plate7-2-O1-A4


title: Pool154_Plate7-2-O1-A3


title: Pool154_Plate7-2-O1-B3


title: Pool154_Plate7-1-O1-P2


title: Pool154_Plate7-2-O1-B16


title: Pool154_Plate7-2-O1-B15


title: Pool154_Plate7-1-O1-P13


title: Pool154_Plate7-1-O1-O1


title: Pool154_Plate7-1-O1-O2


title: Pool154_Plate7-1-O1-O13


title: Pool154_Plate7-1-O1-P1


title: Pool154_Plate7-1-O1-N1


title: Pool154_Plate7-2-O1-B4


title: Pool154_Plate7-1-O1-N14


title: Pool154_Plate7-1-O1-N13


title: Pool154_Plate7-1-O1-N2


title: Pool154_Plate7-1-O1-G1


title: Pool154_Plate7-1-O1-K1


title: Pool154_Plate7-1-O1-I13


title: Pool154_Plate7-1-O1-K2


title: Pool154_Plate7-1-O1-H13


title: Pool154_Plate7-1-O1-M13


title: Pool154_Plate7-1-O1-L13


title: Pool154_Plate7-1-O1-G14


title: Pool154_Plate7-1-O1-M14


title: Pool154_Plate7-1-O1-K13


title: Pool154_Plate7-1-O1-I1


title: Pool154_Plate7-1-O1-L14


title: Pool154_Plate7-1-O1-L2


title: Pool154_Plate7-1-O1-G13


title: Pool154_Plate7-1-O1-H14


title: Pool154_Plate7-1-O1-L1


title: Pool154_Plate7-1-O1-H2


title: Pool154_Plate7-1-O1-J2


title: Pool154_Plate7-1-O1-H1


title: Pool154_Plate7-1-O1-F2


title: Pool154_Plate7-1-O1-F14


title: Pool154_Plate7-1-O1-J1


title: Pool154_Plate7-1-O1-J14


title: Pool154_Plate7-1-O1-J13


title: Pool154_Plate7-1-O1-F1


title: Pool154_Plate7-1-O1-F13


title: Pool154_Plate7-1-O1-G2


title: Pool154_Plate7-1-O1-E2


title: Pool154_Plate7-4-O1-I7


title: Pool154_Plate7-1-O1-E14


title: Pool154_Plate6-6-M1-N11

value: P


title: Pool154_Plate7-1-O1-E13

value: brain


title: Pool154_Plate7-1-O1-E1


title: Pool154_Plate6-6-M1-M23


title: Pool154_Plate6-6-M1-L24


title: Pool154_Plate7-1-O1-A1


title: Pool154_Plate6-6-M1-N24


title: Pool154_Plate6-6-M1-O12


title: Pool154_Plate6-6-M1-P23


title: Pool154_Plate7-1-O1-D14


title: Pool154_Plate7-1-O1-A2


title: Pool154_Plate7-1-O1-C14


title: Pool154_Plate7-1-O1-B14


title: Pool154_Plate7-1-O1-D13


title: Pool154_Plate6-6-M1-L12


title: Pool154_Plate6-6-M1-L23


title: Pool154_Plate7-3-O1-B6


title: Pool154_Plate6-6-M1-O11


title: Pool154_Plate6-6-M1-P24


title: Pool154_Plate7-1-O1-B1


title: Pool154_Plate7-1-O1-A13


title: Pool154_Plate7-1-O1-C2


title: Pool154_Plate7-1-O1-B13


title: Pool154_Plate7-1-O1-D1


title: Pool154_Plate7-1-O1-C13


title: Pool154_Plate7-1-O1-D2


title: Pool154_Plate6-6-M1-L11


title: Pool154_Plate6-6-M1-J24


title: Pool154_Plate6-6-M1-J23


title: Pool154_Plate6-6-M1-K23


title: Pool154_Plate6-6-M1-H24


title: Pool154_Plate6-6-M1-J11


title: Pool154_Plate6-6-M1-H23


title: Pool154_Plate7-1-O1-B2


title: Pool154_Plate6-6-M1-G24


title: Pool154_Plate6-6-M1-G23


title: Pool154_Plate6-6-M1-G12


title: Pool154_Plate6-6-M1-H12


title: Pool154_Plate6-6-M1-D24


title: Pool154_Plate6-6-M1-E23


title: Pool154_Plate6-6-M1-D23


title: Pool154_Plate6-6-M1-A24


title: Pool154_Plate6-6-M1-B23


title: Pool154_Plate6-6-M1-F24


title: Pool154_Plate6-5-M1-P9


title: Pool154_Plate6-6-M1-F23


title: Pool154_Plate6-6-M1-D12


title: Pool154_Plate6-6-M1-E24


title: Pool154_Plate6-6-M1-C23


title: Pool154_Plate6-5-M1-P21


title: Pool154_Plate6-5-M1-P22


title: Pool154_Plate6-6-M1-F11


title: Pool154_Plate6-6-M1-D11


title: Pool154_Plate6-6-M1-A23


title: Pool154_Plate6-5-M1-O22


title: Pool154_Plate6-5-M1-N22


title: Pool154_Plate6-6-M1-H11


title: Pool154_Plate6-6-M1-A11


title: Pool154_Plate6-5-M1-L10


title: Pool154_Plate6-5-M1-L21


title: Pool154_Plate6-5-M1-J22


title: Pool154_Plate6-5-M1-O9


title: Pool154_Plate6-5-M1-J21


title: Pool154_Plate6-5-M1-H22


title: Pool154_Plate6-5-M1-J10


title: Pool154_Plate6-5-M1-I22


title: Pool154_Plate6-5-M1-H21


title: Pool154_Plate6-5-M1-C22


title: Pool154_Plate6-5-M1-A10


title: Pool154_Plate6-5-M1-H9


title: Pool154_Plate6-5-M1-D10


title: Pool154_Plate6-5-M1-B9


title: Pool154_Plate6-5-M1-B22


title: Pool154_Plate6-4-M1-P8


title: Pool154_Plate6-5-M1-D21


title: Pool154_Plate6-5-M1-L22


title: Pool154_Plate6-5-M1-B10


title: Pool154_Plate6-5-M1-D9


title: Pool154_Plate6-4-M1-P19


title: Pool154_Plate6-5-M1-N21


title: Pool154_Plate6-4-M1-P7


title: Pool154_Plate6-4-M1-O7


title: Pool154_Plate6-4-M1-N8


title: Pool154_Plate6-5-M1-H10


title: Pool154_Plate6-4-M1-L7


title: Pool154_Plate6-4-M1-M7


title: Pool154_Plate6-5-M1-D22


title: Pool154_Plate6-5-M1-C21


title: Pool154_Plate6-4-M1-N19


title: Pool154_Plate6-5-M1-A22


title: Pool154_Plate6-4-M1-L19


title: Pool154_Plate6-4-M1-K7


title: Pool154_Plate6-4-M1-J7


title: Pool154_Plate6-4-M1-J8


title: Pool154_Plate6-4-M1-F8


title: Pool154_Plate6-4-M1-G7


title: Pool154_Plate6-4-M1-F7


title: Pool154_Plate6-4-M1-H19


title: Pool154_Plate6-4-M1-F20


title: Pool154_Plate6-4-M1-J19


title: Pool154_Plate6-4-M1-H20


title: Pool154_Plate6-4-M1-J20


title: Pool154_Plate6-4-M1-D7


title: Pool154_Plate6-4-M1-D8


title: Pool154_Plate6-4-M1-D19


title: Pool154_Plate6-4-M1-D20


title: Pool154_Plate6-4-M1-E7


title: Pool154_Plate6-4-M1-C7


title: Pool154_Plate6-4-M1-B8


title: Pool154_Plate6-4-M1-A7


title: Pool154_Plate6-3-M1-P6


title: Pool154_Plate6-3-M1-P5


title: Pool154_Plate6-3-M1-P17


title: Pool154_Plate6-3-M1-P18


title: Pool154_Plate6-3-M1-O6


title: Pool154_Plate6-3-M1-O5


title: Pool154_Plate6-3-M1-N6


title: Pool154_Plate6-6-M1-B24


title: Pool154_Plate6-3-M1-N5


title: Pool154_Plate6-4-M1-I7


title: Pool154_Plate6-3-M1-L5


title: Pool154_Plate6-3-M1-L18


title: Pool154_Plate6-3-M1-K6


title: Pool154_Plate6-3-M1-L17


title: Pool154_Plate6-3-M1-K18


title: Pool154_Plate6-3-M1-I5


title: Pool154_Plate6-3-M1-H17


title: Pool154_Plate6-3-M1-G6


title: Pool154_Plate6-3-M1-H6


title: Pool154_Plate6-3-M1-H18


title: Pool154_Plate6-3-M1-I6


title: Pool154_Plate6-3-M1-F18


title: Pool154_Plate6-3-M1-G5


title: Pool154_Plate6-3-M1-F6


title: Pool154_Plate6-3-M1-M5


title: Pool154_Plate6-3-M1-M17


title: Pool154_Plate6-3-M1-F17


title: Pool154_Plate6-3-M1-E6


title: Pool154_Plate6-3-M1-J18


title: Pool154_Plate6-3-M1-K5


title: Pool154_Plate6-3-M1-C5


title: Pool154_Plate6-3-M1-D5


title: Pool154_Plate6-3-M1-B18


title: Pool154_Plate6-3-M1-E5


title: Pool154_Plate6-3-M1-B6


title: Pool154_Plate6-3-M1-B5


title: Pool154_Plate6-3-M1-D18


title: Pool154_Plate6-3-M1-A5


title: Pool154_Plate6-3-M1-A18


title: Pool154_Plate6-3-M1-A6


title: Pool154_Plate6-2-M1-O3


title: Pool154_Plate6-2-M1-O4


title: Pool154_Plate6-2-M1-N3


title: Pool154_Plate6-2-M1-N4


title: Pool154_Plate6-2-M1-P4


title: Pool154_Plate6-2-M1-L4


title: Pool154_Plate6-2-M1-M4


title: Pool154_Plate6-2-M1-M3


title: Pool154_Plate6-3-M1-J17


title: Pool154_Plate6-2-M1-L16


title: Pool154_Plate6-2-M1-L15


title: Pool154_Plate6-2-M1-K4


title: Pool154_Plate6-2-M1-I3


title: Pool154_Plate6-2-M1-J15


title: Pool154_Plate6-2-M1-I4


title: Pool154_Plate6-2-M1-H3


title: Pool154_Plate6-2-M1-H16


title: Pool154_Plate6-2-M1-P3


title: Pool154_Plate6-2-M1-L3


title: Pool154_Plate6-2-M1-H15


title: Pool154_Plate6-2-M1-G3


title: Pool154_Plate6-2-M1-F4


title: Pool154_Plate6-2-M1-G4


title: Pool154_Plate6-2-M1-F3


title: Pool154_Plate6-2-M1-E3


title: Pool154_Plate6-2-M1-F15


title: Pool154_Plate6-2-M1-D16


title: Pool154_Plate6-2-M1-F16


title: Pool154_Plate6-2-M1-D4


title: Pool154_Plate6-2-M1-C4


title: Pool154_Plate6-2-M1-B16


title: Pool154_Plate6-2-M1-C3


title: Pool154_Plate6-2-M1-D15


title: Pool154_Plate6-1-M1-P2


title: Pool154_Plate6-2-M1-A4


title: Pool154_Plate6-1-M1-P1


title: Pool154_Plate6-1-M1-P14


title: Pool154_Plate6-1-M1-P13


title: Pool154_Plate6-2-M1-B15


title: OIR,P17,antagomir nc 3

city: Shen Yang

first: Tian

middle: Qi

last: Cheng

state: Liao ning

address: Qiuyuehu Street, Shenbei New District

country: China

institute: Chinese Medical University

library_source: transcriptomic

data_processing:
CLC Genomics Workbench v 11.0.1
Sequence reads were trimmed for adaptor sequence/low-quality sequence using CLC genomic benchwork (parameter- Quality limit: 0.05)
Trimmed sequence reads were mapped to GrCM38/mm10 using CLC genomic benchwork (parameters- mismath cost: 2 insertion cost: 3 deletion cost: 3 length fraction: 0.8 similarity fraction: 0.8)
Read count extraction and normalization were performed using CLC genomic benchwork
Assembly: mm10
Supplementary files format and content: tab-delimited text files include RPKM values for each Sample

molecule: total RNA

source_name: retinal

growth_protocol: On the postnatal day 7(P7), the mice were placed in the homemade oxygen tank together with the mothers, and the oxygen concentration was maintained at 75% ± 2% with measured by oximeter.

extract_protocol:
Total RNA from mice retinal tissue was extracted according to the instructions provided by TRNzol reagent，the RNA-seq library was prepared and sequenced in Novagene company.
RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.

treatment_protocol: At P12, OIR mice were intravitreal injected with 1 nmol miR-381-3p antagomir (n=3) or 1 nmol antagomir nc (n=3)

value: retinal

tag: treatment

value: hyperoxia， antagomir nc


title: OIR,P17,antagomir nc 2


title: OIR,P17,miR-381-3p antagomir 3

value: hyperoxia，miR-381-3p antagomir


title: OIR,P17,miR-381-3p antagomir 2


title: OIR,P17,miR-381-3p antagomir 1


title: shrimp hemolymph at 6 hours post-treatment with pirAB toxin, biological rep5

city: Auburn

first: Jason

last: Abernathy

state: AL

address: 990 Wire Road

institute: USDA-ARS

data_processing:
Base-calling and demultiplexing were performed using the native Illumina software by the sequencing service provider.
Raw reads were processed for quality control using FastQC v0.11.9 and TrimGalore v0.6.6. This software was used to trim low-quality ends from reads and for Illumina adapter removal.
RSEM v1.3.1 was used for alignment of reads to the GCF_003789085.1 coding sequences using the alignment parameters recommended by the RSEM software.
Effective read counts output from the RSEM v1.3.1 software were rounded and used to calculate fold-change using the DESeq2 package 1.24.0 with Bioconductor 1.30.10 in R version 3.6.3.
Assembly: All coding sequences from Pacific whiteleg shrimp genomic FASTA (assembly  GCF_003789085.1) in NCBI RefSeq.
Supplementary files format and content: Tab-delimited text files of effective read counts, rounded to the nearest whole number

organism: Penaeus vannamei

source_name: hemolymph

extract_protocol:
Tissue samples were placed in RNAlater and stored at -80°C until usage. Total RNA was extracted with the Qiagen RNeasy Mini Kit per the manufacturer's recommendation.
Total RNA was standardized to 100 ng each sample. Sequencing libraries were then prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with the NEBNext Poly(A) mRNA Magnetic Isolation Module. Barcodes used were the NEBNext Multiplex Oligos for Illumina (Index Primers Sets 1, 2 and 3).

tag: time post pathogen challenge


title: shrimp hemolymph at 6 hours post-treatment with pirAB toxin, biological rep4


title: shrimp hemolymph at 6 hours post-treatment with pirAB toxin, biological rep3


title: shrimp hemolymph at 6 hours post-treatment with pirAB toxin, biological rep2


title: lbSSCs biol rep 4

city: New York

first: Genomics-core-facility

last: WCM

state: New York

address: 1300 York Ave

institute: Weill Cornell Medicine

description: 29 (sample column in fpkm.genes.ensembl_id.protein_coding.txt)

data_processing:
The samples were sequenced using Illumina HiSeq4000 sequencer with paired-end 50 bps reads. The raw sequencing reads in BCL format were processed through bcl2fastq 2.19 (Illumina) for FASTQ conversion and demultiplexing.
The RNA reads were aligned and mapped to the GRCm38 human reference genome by STAR (Version2.5.2).
The transcriptome reconstruction was performed by Cufflinks (Version 2.1.1). The abundance of transcripts was measured with Cufflinks in Fragments Per Kilobase of exon model per Million mapped reads (FPKM).
Assembly: GRCm38
Supplementary files format and content: tab-delimited text file include normalized abundance measurements(FPKM) at gene level for each samples

source_name: femur+tibia

extract_protocol:
RNA was harvested using Rneasy mini plus kit (Qiagen). RNA quality and quantity were verified by using an Agilent 2100 Bioanalyzer system.
cDNA libraries were generated using the Illumina SMART-Seq v4 plus Nextera XT DNA Library Preparation Kit

value: femur+tibia

tag: cell type

value: primary long bone cells

tag: cell fraction

tag: genotype

value: WT

tag: developmental stage

value: postnatal day 10


title: lbSSCs biol rep 1

description: 17 (sample column in fpkm.genes.ensembl_id.protein_coding.txt)

value: male


title: lbSSCs biol rep 3

description: 25 (sample column in fpkm.genes.ensembl_id.protein_coding.txt)

value: female


title: lbSSCs biol rep 2

description: 21 (sample column in fpkm.genes.ensembl_id.protein_coding.txt)


title: candidate vSSCs biol rep 3

description: 10 (sample column in fpkm.genes.ensembl_id.protein_coding.txt)

source_name: spine

value: spine

value: primary vertebral cells


title: candidate vSSCs biol rep 4

description: 14 (sample column in fpkm.genes.ensembl_id.protein_coding.txt)


title: shrimp hemolymph at 4 hours post-treatment with pirAB toxin, biological rep4


title: shrimp hemolymph at 4 hours post-treatment with pirAB toxin, biological rep5


title: candidate vSSCs biol rep 2

description: 6 (sample column in fpkm.genes.ensembl_id.protein_coding.txt)


title: shrimp hemolymph at 6 hours post-treatment with pirAB toxin, biological rep1


title: shrimp hemolymph at 4 hours post-treatment with pirAB toxin, biological rep2


title: OIR,P17,antagomir nc 1


title: shrimp hemolymph at 2 hours post-treatment with pirAB toxin, biological rep5


title: candidate vSSCs biol rep 1

description: 2 (sample column in fpkm.genes.ensembl_id.protein_coding.txt)


title: shrimp hemolymph at 2 hours post-treatment with pirAB toxin, biological rep4


title: shrimp hemolymph at 2 hours post-treatment with pirAB toxin, biological rep3


title: MS blastocyst IP Rep1

city: Ann Arbor

first: Kin Fai

last: Au

state: MI

address: 100 Washtenaw Avenue

department: Department of Computational Medicine and Bioinformatics

institute: University of Michigan

data_processing:
Quality of raw reads was assessed using FastQC (v0.11.8) and sequencing adapters were trimmed using Cutadapt (v1.8.1) if clear adapter signal was detected at the 3'end of the reads. There is no adapter removal for zebrafish zygote samples (GSM5599713, GSM5599714, GSM5599715, GSM5599716, GSM5599717, GSM5599718, GSM5599719, GSM5599720, GSM5599721, GSM5599722, GSM5599723, GSM5599724, GSM5599725), mouse polyA-selected RNA samples (GSM5599726, GSM5599727, GSM5599728, GSM5599729, GSM5599730, GSM5599731, GSM5599732, GSM5599733), and mouse MII oocyte Input sample (GSM5599734).
Trimmed reads were aligned to the reference genome (mm10 for mouse, danRer11 for zebrafish) using HISAT2 (v2.1.0) with the parameter “-5 8 --no-mixed --no-discordant”. Multiply-aligned reads were discarded as indicated by HISAT2. PCR duplicates were removed using Samtools (v1.9). The read pairs that overlapped with ribosomal RNAs were removed using BEDTools (v2.28.0).
Genome coverage bigWig files (bin size = 10 bp, normalized by RPKM) were generated by deepTools (v3.2.0) bamCoverage with the parameter “-bs 10 --normalizeUsing RPKM”.
Genome_build: mm10 for mouse, danRer11 for zebrafish
Supplementary_files_format_and_content: The bigWig files are generated by deepTools (v3.2.0) bamCoverage with the bin size=10bp and normalized by RPKM.

source_name: blastocyst

extract_protocol:
For zebrafish bulk-zygote samples, total RNA was extracted from 100 zebrafish zygotes using TRIzol™ Reagent and eluated in 100 μl of RNase-free water. Then, 10 μl and 5 μl samples were taken for 10-zygote and 5-zygote samples, respectively, and volumes were adjusted to 12 μl with nuclease-free water. For zebrafish single-zygote samples, single zygote was manually picked and distributed into 12 μl of 1X Lysis buffer (Takara).
For the polyA-selected RNA samples of mouse liver and embryonic stem cell (WT and Mettl3 KO), total RNA was extracted using TRIzol™ Reagent (Thermo Fisher Scientific) and PolyA+ RNA was selected twice by Dynabeads™ mRNA Purification Kit (Thermo Fisher Scientific).
For mouse embryonic stem cell samples, the cells were sorted into 12 μl 1X Lysis buffer using BD FACSMelody™ Cell Sorter (BD Biosciences).
For mouse oocyte and embryo samples, after three washes in M2 medium, oocytes and embryos were manually picked and put into 12 μl 1X Lysis buffer.
For picoMeRIP-seq experiment, NEBNext® rRNA Depletion Kit (NEB) was used to delete DNA and rRNA; RNA samples were sheared by sonication, incubated with the anti-m6A antibody from Millipore (ABE572), and precipitated using ethanol.
SMART-Seq® Stranded Kit (Takara, Cat. # 634442) was used to prepare RNA sequencing library. Libraries were quantified with KAPA Library Quantification Kits (Roche) and the size distribution was checked using TapeStation D1000 ScreenTape (Agilent Technologies).

treatment_protocol:
For mouse GV oocyte collection, 8-week-old C57BL6/N females were injected with 5 units (U) of pregnant mare serum gonadotropin (PMSG). After 48 hours, ovaries were dissected and oocytes were isolated by puncturing the follicles. The procedure was carried out in M2 medium (Sigma) supplemented with 0.2 mM of the cyclic nucleotide phosphodiesterase inhibitor 3-Isobutyl-1-methylxanthine (IBMX, Sigma) to prevent the oocytes from further progress to germinal vesicle breakdown (GVBD). The cumulus cells were gently removed by pipetting, and the oocytes were briefly exposed to acidic Tyrode's solution (Sigma) to remove the zona pellucida, followed by three washes in M2 medium.
For mouse MII oocyte collection, 4-5-week-old C57BL6/N females were injected with 5 U of PMSG followed by 5 U of human chorionic gonadotropin (hCG) 45 hours later. The oviducts were dissected 20-22 hours later and transferred to a clean dish containing M2 medium. The oviduct ampulla was identified under a stereomicroscope to isolate MII oocytes containing the cumulus mass. The oocytes were treated with 0.3 mg/ml hyaluronidase dissolved in M2 medium to remove the cumulus cells, and then exposed to acidic Tyrode's solution for a few seconds to remove the zona pellucida, followed by three washes in M2 medium.
For mouse early embryo collection, 8-week-old C57BL/6N females were superovulated by hormone injection (5 U PMSG followed by 5 U hCG 45 hours later) and transferred to cages with 8-week-old C57BL/6N males for mating. At 27-28 hours (zygote), 39-43 hours (2-cell), 68-70 hours (8-cell), and 92-94 hours (blastocyst) after hCG administration, the female mice were euthanized by cervical dislocation. Embryos were flushed from the reproductive tract into HEPES-buffered CZB medium, then transferred to acidic Tyrode's solution for a few seconds to remove the zona pellucida, followed by three washes in M2 medium.

value: blastocyst

tag: strain

tag: molecule subtype

value: rRNA-free RNA


title: MS 8cell IP Rep1

source_name: eight-cell embryo

value: eight-cell embryo


title: MS 8cell IP Rep2


title: MS blastocyst IP Rep2


title: MS 2cell IP Rep2

source_name: two-cell embryo

value: two-cell embryo


title: shrimp hemolymph at 2 hours post-treatment with pirAB toxin, biological rep2


title: MS 2cell IP Rep1


title: MS zygote IP Rep2

source_name: zygote

value: zygote


title: shrimp hemolymph at 1 hour post-treatment with pirAB toxin, biological rep4


title: shrimp hemolymph at 2 hours post-treatment with pirAB toxin, biological rep1


title: shrimp hemolymph at 1 hour post-treatment with pirAB toxin, biological rep5


title: shrimp hemolymph at 1 hour post-treatment with pirAB toxin, biological rep3


title: MS zygote IP Rep1


title: MS MII IP Rep2

source_name: MII oocyte

value: MII oocyte


title: MS MII IP Rep1


title: shrimp hemolymph at 1 hour post-treatment with pirAB toxin, biological rep2


title: MS GV IP Rep1

source_name: GV oocyte

value: GV oocyte


title: MS GV IP Rep2


title: shrimp hemolymph at 1 hour post-treatment with pirAB toxin, biological rep1


title: shrimp hemolymph at 30 minutes post-treatment with pirAB toxin, biological rep5


title: shrimp hemolymph at 4 hours post-treatment with pirAB toxin, biological rep3


title: shrimp hemolymph at 4 hours post-treatment with pirAB toxin, biological rep1


title: shrimp hemolymph at 30 minutes post-treatment with pirAB toxin, biological rep4


title: shrimp hemolymph at 30 minutes post-treatment with pirAB toxin, biological rep2


title: shrimp hemolymph at 30 minutes post-treatment with pirAB toxin, biological rep3


title: MS liver 6ng, WT ESC 6ng, spike in, Input

source_name: liver

value: liver

value: embryonic stem cells

value: Wild type mESC

value: polyA RNA


title: MS liver 6ng, KO ESC 6ng, spike in, Input

value: Mettl3 knock out mESC


title: MS liver 6ng, KO ESC 6ng, spike in, IP


title: shrimp hemolymph at 30 minutes post-treatment with pirAB toxin, biological rep1


title: shrimp hemolymph at 30 minutes post-treatment with buffer only, control biological rep5


title: shrimp hemolymph at 30 minutes post-treatment with buffer only, control biological rep4


title: MS liver 12ng, spike in, IP


title: MS liver 12ng, spike in, Input


title: MS ESC IP 10cell Rep3

tag: cell line


title: shrimp hemolymph at 30 minutes post-treatment with buffer only, control biological rep3


title: MS ESC IP 10cell Rep2


title: shrimp hemolymph at 30 minutes post-treatment with buffer only, control biological rep2


title: shrimp hemolymph at 30 minutes post-treatment with buffer only, control biological rep1


title: soybean-root_t40min-sucrose_biol-rep2

city: Hayward

first: Claudia

last: Uhde-Stone

address: 25800 Carlos Bee Blvd

department: Biological Sciences

institute: California State University, East Bay

description:
CUSGm01_H
150 bp PE

data_processing:
Illumina bcl2fastq2(v2.20) Conversion Software was used to demultiplex the obtained sequencing data and to convert base call files into FASTQ files.
Quality of sequences were checked by FastQC; adaptors and any low-quality sequences were removed with TRIMMOMATIC Version0.32
All paired RNA-seq reads were mapped to the reference genome of G. max cultivar Williams 82 using HISAT2,  followed by StringTie
We used the prepDE.py3 script (http://ccb.jhu.edu/software/stringtie/dl/prepDE.py3) to extract two csv (comma separated value) files with transcript and gene count information.
The normalized expression data FPKM (Fragment per kilobase and million) was extracted using the R package Ballgown
Assembly: Glycine max (assembly Glycine_max_v4.0)
Supplementary files format and content: normalized expression data FPKM (Fragment per kilobase and million) from all 9 cDNA libraries (t0, t20, t40, in 3 biologiical repliucations), extracted using the R package Ballgown

organism: Glycine max

source_name: 2 cm root sections with roottip

growth_protocol: Once the radicle reached 3 to 5 cm in length, germinatedsoybean seeds were transferred to containers containing about 850 ml half-strength Hoagland nutrient solution and were grown for 4 weeks;Hoagland solution was changed every 4 days. The growth chamber conditions were maintained at ~27℃ with a light cycle of 16 hours.

extract_protocol:
RNeasy Plant Mini kit (Qiagen) was used for RNA extraction, following the protocol for “Purification of Total RNA from Plant Cells andTissues, and Filamentous Fungi“.
Using the Stranded mRNA Kit (Illumina), we converted the extracted RNA to cDNA, following the manufacturer’s instructions. Unique dual barcoding for each cDNA library was done using the IDT for Illumina RNA UD Indexes Set A (Illumina).

treatment_protocol: For sucrose treatment, 8.5 ml of 1 M sucrose (prepared in half-strength Hoagland solution) was added directly to the hydroponic solution, for a final concentration of 10 mM sucrose.

value: 2 cm root sections with roottip

value: 10 mM sucrose for 40 min


title: MS ESC IP 100cell Rep2


title: MS ESC IP 10cell Rep1


title: soybean-root_t40min-sucrose_biol-rep3

description:
CUSGm01_I
150 bp PE


title: soybean-root_t40min-sucrosel_biol-rep1

description:
CUSGm01_G
150 bp PE


title: MS ESC IP 100cell Rep3


title: MS liver 6ng, WT ESC 6ng, spike in, IP


title: MS ESC IP 100cell Rep1


title: soybean-root_t20min-sucrose_biol-rep2

description:
CUSGm01_E
150 bp PE

value: 10 mM sucrose for 20 min


title: MS ESC IP 1000cell Rep2


title: MS ESC IP 1000cell Rep3


title: MS ESC IP 1000cell Rep1


title: soybean-root_t20min-sucrose_biol-rep1

description:
CUSGm01_D
150 bp PE


title: soybean-root_t0-control_biol-rep3

description:
CUSGm01_C
150 bp PE

value: no sucrose added


title: MS ESC Input 1000cell Rep1


title: HSC_LAC_rep3

city: Chicago

first: Nissim

last: Hay

state: IL

address: 900 South Ashland Ave

department: BMG

institute: University of Illinois Chicago

data_processing:
Quality check was done using FastQC v0.11.8
Sequenced reads were trimmed using cutadapt v1.18
Abundance of transcripts was quantified by kallisto v0.44.0 using kallisto index created from mm10
Read counts were normalized to counts per million reads (CPM) using edgeR v3.8
Assembly: mm10
Supplementary files format and content: tab delimited-text file including CPM values from each sample

source_name: Liver

growth_protocol: Mouse primary HSCs were isolated using density gradient centrifugation. Freshly isolated cells from mouse liver were considered quiescent HSCs (QS). To obtain activated mouse primary HSCs, cells were maintained for 6 days in Dulbecco’s modified Eagle’s medium (DMEM) containing 1,000 mg/L glucose supplemented with 10% fetal bovine serum and 1% penicillin‒streptomycin at 37 °C in a humidified atmosphere with 5% CO2.

extract_protocol:
Total RNA was extracted from mouse primary HSCs using a Direct-zol RNA MicroPrep Kit. The RNA purity, concentration, and integrity were evaluated with an Agilent TapeStation 4200.
RNA libraries were prepared using Illumina TruSeq Stranded mRNA sample library preparation kit.

treatment_protocol: Mouse primary HSCs from Hk2 fl/fl mouse liver were transduced by adenovirus expressing either eGFP (ACT) or Cre recombinase fused to eGFP to delete Hk2 (KO), and cultured for 6 days. KO cells were treated with 10 mM lactate (LAC) for 24 h.

value: Liver

value: Hepatic stellate cell

value: Hk2 fl/fl

value: Ad-Cre + Lactate


title: soybean-root_t0-control_biol-rep2

description:
CUSGm01_B
150 bp PE


title: HSC_LAC_rep2


title: 011 (Pdpn-adjacent)

city: Charlottesville

first: Anwaruddin

last: Mohammad

state: VA

address: Pinn Hall

department: Bioinformatics Core

institute: University of Virginia

data_processing: Sequencing libraries were generated by PCR from the photo-released indexing oligos and AOI-specific Illumina adapter sequences. Each PCR reaction used 4 μl of indexing oligonucleotides, 4 μl of indexing PCR primers, 2 μl of Nanostring 5X PCR Master Mix. Thermocycling conditions were 37 °C for 30 min, 50 °C for 10 min, 95 °C for 3 min; 18 cycles of 95 °C for 15 s, 65 °C for 1 min, 68 °C for 30 s; and 68 °C for 5 min. PCR reactions were pooled, and pooled libraries were paired-sequenced  on an Illumina Next Seq 2000 instrument. FASTQ files for each sample were converted to digital count conversion (DCC) files using the GeoMx DnD pipeline (v.1) of Nanostring according to manufacturer’s pipeline. DCC files were imported back into the GeoMx DSP instrument for QC and data analyses using GeoMx DSP analysis suite version 2.2.0.111 (Nanostring). A minimum of 10,000 reads were required for each sample. Probes were checked for outlier status by implementing a global Grubb’s outlier test with alpha set to 0.01. The counts for all remaining probes for a given target were then collapsed into a single metric by taking the geometric mean of probe counts. For each sample, an RNA-probe-pool-specific negative probe normalization factor was generated on the basis of the geometric mean of negative probes in each pool. To ensure good data quality, we calculated the 75th percentile of the gene counts (that is, geometric mean across all non-outlier probes for a given gene) for each AOI, and normalized to the geometric mean of the 75th percentile across all AOIs to give the upper quartile (Q3) normalization factors for each AOI. The distribution of these Q3 normalization factors were then checked for outliers. Samples 003 (Pdpn-adjacent) and 006 (SMA-adjacent) were excluded from final analysis as they did not pass quality control checks.

source_name: mammary gland

growth_protocol: Paraformaldehyde-fixed frozen blocks

extract_protocol:
Slides were imaged at 20x magnification using Nanostring GeoMx DSP platform and morphologic markers SMA and Pdpn were used to select regions of interest (ROIs). Automatic segmentation of the ROIs based on ZSGreen expression were used to define areas of interest (AOIs), allowing for the separation of ZSGreen+ periostin-expressing cells from surounding cells. A total of 12 AOIs were exposed to 385 nm (UV) light, releasing the index oligonucleotides which were collected with a microcapillary and deposited into individual wells of a 96-well plate for subsequent processing. The indexing oligonucleotides were dried overnight and resuspended in 10 uL of DEPC-treated water.
Library was constructed at University of Minnesota Genomics Center

treatment_protocol: Fixed frozen mammary glands from 3 mice were incubated with the NanoString mouse Whole Transcriptome Atlas (WTA) panel probes overnight, then stained with Cy3-SMA (TCF C6198), Pdpn (R&D Systems AF3244;Cy5 secondary), and the DNA stain Syto83 (Thermo S11364) as morphology markers to visualize tissue architecture. Stained slides were loaded onto the GeoMx instrument and scanned for region of interest (ROI) selection.

value: mammary gland


title: 009 (SMA-adjacent)


title: 010 (SMA-adjacent)


title: HSC_LAC_rep1


title: soybean-root_t20min-sucrose_biol-rep3

description:
CUSGm01_F
150 bp PE


title: 008 (Pdpn-adjacent)


title: 006 (SMA-adjacent)


title: 007 (Pdpn-adjacent)


title: HSC_KO_rep2

value: Ad-Cre


title: 004 (Pdpn-adjacent)


title: HSC_KO_rep3


title: 005 (SMA-adjacent)


title: HSC_KO_rep1


title: MS ESC Input 1000cell Rep2

value: rRNA-free RNA


title: 003 (Pdpn-adjacent)


title: 002 (Pdpn-adjacent)


title: soybean-root_t0-control_biol-rep1

description:
CUSGm01_A
150 bp PE


title: HSC_ACT_rep3

value: Ad-GFP