title: SpcT, replicate 1, scRNAseq

city: Stanford

middle: D

last: Attardi

address: 269 Campus Drive

institute: Stanford University

description: 10x Genomics

data_processing:
Analyses were performed in R (version 4.0.3) using various R packages and Python (version 3.6) unless otherwise noted using the Stanford SCG Informatics Cluster. FASTQ files were processed using cellranger count (10x genomics). This pipeline aligns reads to the mm10 mouse reference genome, performs barcode error correction, PCR duplicate marking, barcode counting, and UMI counting to produce expression count matrices.  The gene expression matrices were loaded into R and processed with Seurat (version 4.0.1, https://github.com/satijalab/seurat) for downstream analyses.
Assembly: mm10
Supplementary files format and content: filtered feature matrix CellRanger output

source_name: lung

extract_protocol:
SpcT and SpcPT mice treated as described above. Due to mouse colony limitations, one SpcT mouse was Sftpc-CreERT2;p53+/+;Rosa26Confetti (Brainbow 2.1)85. However, RFP is only expressed after Cre treatment, as in SpcT mice, and only red RFP+ cells were sorted from this mouse in an identical manner as the replicate SpcT mouse. 96 hours after BHT treatment, lungs were harvested and perfused with 10 mL of ice-cold PBS and placed in a sterile dish on ice. Lungs were minced with sterile scissors and resuspended in 6 mL digestion media (RPMI with 0.083 U/mL of collagenase type IV (Sigma-Aldrich), 0.6 U/mL dispase ii (Sigma-Aldrich), and 25 µg/mL DNase (Sigma Aldrich)).  Lungs were rotated for 30 minutes at 37ºC. Tubes were briefly cooled on ice, and then samples were passed through a 70 µM filter followed by a 40 µM filter. 5 mL of FACS buffer (10% FBS, 2 mM EDTA, 25 µg/mL DNase in DPBS) was added and tubes were spun for 5 minutes at 300g. Cells were resuspended in ACK lysing buffer (Gibco), incubated for 1 minute on ice, and quenched with 8 mL of FACS buffer. Cells were washed 2x with FACS buffer, resuspended with biotinylated primary antibodies (CD45, BioLegend 103104 30-F11; CD31, BioLegend 102404 390; F4/80, BioLegend 123106 BM8; Ter119, BioLegend 116204 TER-119, 1:800 in FACS buffer) and incubated for 20 minutes on ice. After washing 2x, cells were resuspended in streptavidin-APC secondary antibody (BioLegend 405207, 1:800) for 20 minutes on ice. Cells were washed 2x and resuspended in FACS buffer with 1 µg/mL DAPI, filtered through a 40 µM filter, and sorted using a Sony SH800S cell sorter. Data were analyzed using the default Sony SH800S software and FCS Express (Version 7, De Novo Software).
Single cell suspensions were prepared from mice treated with BHT as described above. Libraries were prepared using the 10x Genomics Single Cell 3’ Library v3.1 kit according to the manufacturer’s protocols. An input of 10,000 cells were added to each 10x channel. Libraries were sequenced on an Illumina NovaSeq6000.

value: lung

value: SpcT


title: embryo_cells_rep1

status: Public on May 04 2023

type: SRA

city: Fort Collins

first: Erin

last: Osborne Nishimura

state: CO

address: 1870 Campus Delivery, 200 W. Lake St.

department: Biochemistry and Molecular Biology

country: USA

institute: Colorado State University

description:
RW057
embryo_dissociated_cells_merged.bw

library_source: transcriptomic

data_processing:
Samples were assessed for quality and trimmed for adapter, primer, and polyX sequences by fastp (v 0.20.0) (Chen 2017; PMID: 30423086)
Trimmed reads were aligned separately to the ce11 genome using hisat2 (v 2.1.0) (Kim 2017; PMID: 31375807).
The number of reads associated with each gene model was tabulated for the C. elegans WS263 genome annotation using featureCounts (v 1.6.4) (Liao 2014; PMID: 24227677).
samtools (v 1.9) (Li 2009; PMID: 19505943), DeepTools (bamCoverage v 3.2.0) (Ramírez 2016; PMID: 27079975), and UCSC Kent Utilities were used to transform alignments into formats suitable for viewing on UCSC Genome Browser (Kent 2002; PMID: 12045153).
RNA-seq count data were filtered for detected genes (> 10 counts per million across all samples) and analyzed using DESeq2 (v 1.28.1) (Love 2014; PMID: 25516281) with default settings in the R Environment (v 4.0.3, BiocManager, v 1.30.10).
The raw counts (intestine_FACS_RNAseq_raw_counts.txt), normalized counts (intestine_FACS_RNAseq_norm_counts.txt), and normalized/variance-stabilized/log-transformed counts (intestine_FACS_RNAseq_rlog_counts.txt) are provided.
Assembly: C. elegans, ce11
Supplementary files format and content: .gtf file: c_elegans.PRJNA13758.WS263.canonical_geneset.gtf
Supplementary files format and content: .bw files were generated using bamCoverage, part of the DeepTools package
Supplementary files format and content: merged.bw files were merged by replicate using UCSC Kent Utilities
Supplementary files format and content: counts tables were generated from featureCounts tabultion and then processed within DESeq2: intestine_FACS_RNAseq_norm_counts.txt; intestine_FACS_RNAseq_raw_counts.txt; intestine_FACS_RNAseq_rlog_counts.txt

molecule: total RNA

organism: Caenorhabditis elegans

source_name: dissociated worm cells

growth_protocol: C. elegans strains were cultered on 150 mm NGM agar plates seeded with 3 ml of OP50 liquid culture. Two rounds of synchronization were performed and then harvested at the approporate stage.

extract_protocol:
Total RNA was extracted using an miRNeasy micro kit (Qiagen 217084).
mRNA was prepared for sequencing using the NEB Next Ultra II RNA Library Prep Kit for Illumina (NEB E7770S) and Poly(A) Magnetic Isolation Module (NEB E7490S).
Libraries of a median 200 - 400 bp length were generated. Libraries RW047-RW055 were sequenced at 2 x 150 bp on a HiSeq X-ten to a read depth of 100 million paired end reads. Libraries RW057-RW089 were sequenced at 2 x 150 bp on NovaSeq 6000 to a read depth of  40 to 100 million paired end reads.

treatment_protocol: Worms were washed 3 times in sterile M9 before cell dissociation to remove excess OP50 E. coli.

tag: tissue

value: dissociated worm cells

tag: strain

tag: cell type

value: all

tag: genotype

tag: Stage

value: embryo

tag: biological replicate

tag: treatment

value: 70ul of cell suspension

value: 20 min chitinase, 5 min pronase E treatment

value: 100 strokes 21 gague syringe needle


title: SpcPT, replicate 1, scRNAseq

first: Laura

state: CA

description: 10x Genomics

library_source: transcriptomic single cell

organism: Mus musculus

value: SpcPT


title: SpcPT, replicate 2, scRNAseq

description: 10x Genomics


title: L3_GFPplus_rep1

description:
RW080
L3_intestine_cells_merged.bw

source_name: sorted intestine cells

value: sorted intestine cells

value: intestine

value: 300,000 GFP+ cells

value: 2 min SDS-DTT treatment, 30 min pronase E treatment

value: 100 strokes dounce pestle A


title: KFT, replicate 2, 10x multiome RNA-Seq

description: 10x Genomics

data_processing:
Analyses were performed in R (version 4.0.3) using various R packages and Python (version 3.6) unless otherwise noted using the Stanford SCG Informatics Cluster. FASTQ files were processed using cellranger-arc count (10x genomics). This pipeline aligns reads to the mm10 mouse reference genome, performs barcode error correction, PCR duplicate marking, peak calling, and ATAC and gene expression molecule counting to produce expression and fragment count matrices.
Assembly: mm10
Supplementary files format and content: filtered feature matrix CellRanger output

molecule: nuclear RNA

extract_protocol:
KFT, KT, and KPT mice were treated with Ad5-SPC-Cre as described above. After 10 weeks, lungs were harvested and were perfused with 10 mL of ice-cold PBS and placed in a sterile dish on ice. Lungs were minced with sterile scissors and resuspended in 6 mL digestion media (RPMI with 0.083 U/mL of collagenase type IV (Sigma-Aldrich), 0.6 U/mL dispase ii (Sigma-Aldrich), and 25 µg/mL DNase (Sigma Aldrich)).  Lungs were rotated for 45 minutes at 37ºC. Tubes were briefly cooled on ice, and then samples were passed through a 70 µM filter followed by a 40 µM filter. 5 mL of FACS buffer (10% FBS, 2 mM EDTA, 25 µg/mL DNase in DPBS) was added and tubes were spun for 5 minutes at 300g. Cells were resuspended in ACK lysing buffer (Gibco), incubated for 1 minute on ice, and quenched with 8 mL of FACS buffer. Cells were washed 2x with FACS buffer, resuspended with biotinylated primary antibodies (CD45, BioLegend 103104 30-F11; CD31, BioLegend 102404 390; F4/80, BioLegend 123106 BM8; Ter119, BioLegend 116204 TER-119, 1:800 in FACS buffer) and incubated for 20 minutes on ice. After washing 2x, cells were resuspended in streptavidin-APC secondary antibody (BioLegend 405207, 1:800) for 20 minutes on ice. Cells were washed 2x and resuspended in FACS buffer with 1 µg/mL DAPI, filtered through a 40 µM filter, and sorted using a Sony SH800S cell sorter. Data were analyzed using the default Sony SH800S software and FCS Express (Version 7, De Novo Software).
Single cell suspensions were prepared from mice with lung tumors as described above. Nuclei were isolated and libraries were prepared using the 10x Genomics Chromium Single Cell Multiome ATAC + Gene Expression kit according to the manufacturer’s protocols. An input of at least 2,000 nuclei were added to each 10x channel. Libraries were sequenced on an Illumina NovaSeq6000.
10x multiome gene expression

value: KFT


title: KT, replicate 2, 10x multiome RNA-Seq

description: 10x Genomics

value: KT


title: KPT, replicate 1, 10x multiome RNA-Seq

description: 10x Genomics

value: KPT


title: KFT, replicate 1, 10x multiome ATAC-Seq

description: 10x Genomics

library_source: genomic single cell

data_processing:
Analyses were performed in R (version 4.0.3) using various R packages and Python (version 3.6) unless otherwise noted using the Stanford SCG Informatics Cluster. FASTQ files were processed using cellranger-arc count (10x genomics). This pipeline aligns reads to the mm10 mouse reference genome, performs barcode error correction, PCR duplicate marking, peak calling, and ATAC and gene expression molecule counting to produce expression and fragment count matrices.  The gene expression matrices and ATAC fragments were loaded into R and processed with Seurat (version 4.0.1, https://github.com/satijalab/seurat) and Signac (version 1.7.0, https://github.com/timoast/signac) for downstream analyses.
Assembly: mm10
Supplementary files format and content: fragment files CellRanger output

molecule: genomic DNA

extract_protocol:
KFT, KT, and KPT mice were treated with Ad5-SPC-Cre as described above. After 10 weeks, lungs were harvested and were perfused with 10 mL of ice-cold PBS and placed in a sterile dish on ice. Lungs were minced with sterile scissors and resuspended in 6 mL digestion media (RPMI with 0.083 U/mL of collagenase type IV (Sigma-Aldrich), 0.6 U/mL dispase ii (Sigma-Aldrich), and 25 µg/mL DNase (Sigma Aldrich)).  Lungs were rotated for 45 minutes at 37ºC. Tubes were briefly cooled on ice, and then samples were passed through a 70 µM filter followed by a 40 µM filter. 5 mL of FACS buffer (10% FBS, 2 mM EDTA, 25 µg/mL DNase in DPBS) was added and tubes were spun for 5 minutes at 300g. Cells were resuspended in ACK lysing buffer (Gibco), incubated for 1 minute on ice, and quenched with 8 mL of FACS buffer. Cells were washed 2x with FACS buffer, resuspended with biotinylated primary antibodies (CD45, BioLegend 103104 30-F11; CD31, BioLegend 102404 390; F4/80, BioLegend 123106 BM8; Ter119, BioLegend 116204 TER-119, 1:800 in FACS buffer) and incubated for 20 minutes on ice. After washing 2x, cells were resuspended in streptavidin-APC secondary antibody (BioLegend 405207, 1:800) for 20 minutes on ice. Cells were washed 2x and resuspended in FACS buffer with 1 µg/mL DAPI, filtered through a 40 µM filter, and sorted using a Sony SH800S cell sorter. Data were analyzed using the default Sony SH800S software and FCS Express (Version 7, De Novo Software).
Single cell suspensions were prepared from mice with lung tumors as described above. Nuclei were isolated and libraries were prepared using the 10x Genomics Chromium Single Cell Multiome ATAC + Gene Expression kit according to the manufacturer’s protocols. An input of at least 2,000 nuclei were added to each 10x channel. Libraries were sequenced on an Illumina NovaSeq6000.
10x multiome ATAC

value: lung


title: KFT, replicate 2, 10x multiome ATAC-Seq

description: 10x Genomics


title: KPT, replicate 2, 10x multiome ATAC-Seq

description: 10x Genomics


title: KT, replicate 1, 10x multiome ATAC-Seq

description: 10x Genomics


title: KT, replicate 1, 10x multiome RNA-Seq

description: 10x Genomics


title: KPT, replicate 2, 10x multiome RNA-Seq

description: 10x Genomics


title: KT, replicate 2, 10x multiome ATAC-Seq

description: 10x Genomics


title: Stomach, Infected Helicobacter pylori_2

city: Changsha

first: Huan

last: Li

state: Hunan

address: 138 Tongzipo Road, Yuelu district

department: Gastroenterology department

country: China

institute: The Third Xiangya Hospital of Central South University

data_processing:
A cDNA library constructed by the pooled RNA was sequenced run with Illumina NovaseqTM 6000 sequence platform.Using the Illumina paired-end RNA-seq approach, we sequenced the transcriptome, generating a total of millon 2 x 150 bp paired-end reads.To get high quality clean reads, reads were further filtered by Cutadapt (https://cutadapt.readthedocs.io/en/stable/,version:cutadapt-1.9)
we aligned reads of all samples to the homo sapiens reference genome(ftp://ftp.ensembl.org/pub/release-101/fasta/gallus_gallus/dna/) using HISAT2(https://daehwankimlab.github.io/hisat2/,version:hisat2-2.0.4) package
The mapped reads of each sample were assembled using StringTie(http://ccb.jhu.edu/software/stringtie/,version:stringtie-1.3.4d) with default parameters. Then, all transcriptomes from all samples were merged to reconstruct a comprehensive transcriptome using gffcompare software(http://ccb.jhu.edu/software/stringtie/gffcompare.shtml,version:gffcompare-0.9.8).
After the final transcriptome was generated, StringTie and ballgown (http://www.bioconductor.org/packages/release/bioc/html/ballgown.html) were used to estimate the expression levels of all transcripts and perform expression abundance for mRNAs by calculating FPKM (fragment per kilobase of transcript per million mapped reads) value.
RNAs differential expression analysis was performed by Deseq2 software between two different group and two different samples. The genes/transcripts with the parameter of pvalue < 0.05 and fold change > 2 or fold change <0.5.
Assembly: hg38
Supplementary files format and content: gene expression text file includes raw counts and FPKM values for each Sample

source_name: stomachs

growth_protocol: Four to five weeks old male C57 BL/6 were housed in an experimental animal room, which meets the specific pathogen-free (SPF)-class Meets the SPF standard, to ensure an environment with 12 h of light and 12 h of darkness.

extract_protocol:
RNA was harvested using RNA 6000 Nano LabChip Kit (Agilent, CA, USA, 5067-1511). 5 ug of total RNA was used for the construction of sequencing libraries.
RNA libraries for RNA-seq were prepared using Dynabeads Oligo (dT) (Thermo Fisher, CA, USA) following manufacturer's protocols.

treatment_protocol: The mice were orally gavaged with 0.3 mL phosphate buffered saline (PBS) with and without H. pylori suspension(1 × 109) in once daily for 9 days (repeat three times with 1 day off for three consecutive days)

value: stomachs

tag: infection

value: Helicobacter pylori


title: Stomach, control_2

value: PBS


title: SpcT, replicate 2, scRNAseq

description: 10x Genomics

value: SpcT


title: Stomach, Infected Helicobacter pylori_1


title: Pool154_Plate6-1-M1-O2

city: La Jolla

first: Joseph

middle: R

last: Ecker

address: 10010 North Torrey Pines Road

department: Genomic Analysis Laboratory

institute: HHMI-Salk-Institute

library_source: genomic

data_processing:
Reads trimed with Cutadapt for adapters and low quality bases (Phred score >= 20)
The resulting files are mapped to in-silico bisulfite converted reference genome using Bismark.
The raw BAM file will be filtered using samtools to filter reads by MAPQ > 10 and use Picard to remove PCR duplicates
Tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls will be generated with in-house developing package using the filtered BAM files
Assembly: mm10
Supplementary files format and content: tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls. Columns are: 1. Chromosome, 2. Position, 3. Strand, 4. Cytosin Context, 5. methylated base call, 6. total mapped base call, 7. not used, place holder column
Library strategy: snmC-seq2 & 3

source_name: brain

growth_protocol: All experimental procedures using live animals were approved by the Salk Institute Animal Care and Use Committee. The knock-in mouse line, R26R-CAG-loxp-stop-loxp-Sun1-sfGFP-Myc (INTACT) was used for most experiments4 and they were maintained on a C57BL/6J background. 42-49 day old adult male and female INTACT mice were used for the retrograde labeling experiment.

extract_protocol:
Approximately two weeks after the AAVretro injection, brains were extracted from the 56-63 day old INTACT mice, immediately submerged in ice-cold slicing buffer (2.5mM KCl, 0.5mM CaCl2, 7mM MgCl2, 1.25mM NaH2PO4, 110mM sucrose, 10mM glucose and 25mM NaHCO3) that was bubbled with carbogen, and sliced into 0.6 mm coronal sections starting from the frontal pole. From each AAVretro-injected brain, the slices were kept in the ice-cold dissection buffer from which selected brain regions (Supplementary Table 1) were manually dissected under a fluorescent dissecting microscope (Olympus SZX16), following the Allen Mouse Common Coordinate Framework (CCF), Reference Atlas, Version 3 (2015) (Extended Data Fig. 1). The dissected brain tissues were transferred to prelabeled microcentrifuge tubes, immediately frozen in dry ice, and subsequently stored at -80oC.   For each dissected brain region, samples from 2 males and 2 females were pooled separately as biological replicates for nuclei preparation. The 2-mL glass tissue dounce homogenizer and pestles (Sigma-Aldrich D8938-1SET) were pre-chilled on ice. Nuclei were prepared using a modified protocol as reported by Lacar et al., 201622. In summary, the frozen brain tissues were transferred to the dounce homogenizer with 1 mL ice-cold NIM buffer (0.25M sucrose, 25mM KCl, 5mM MgCl2, 10mM Tris-HCl (pH7.4), 1mM DTT (Sigma 646563), 10μl of protease inhibitor (Sigma P8340)), with 0.1% Triton X-100 and 5μM Hoechst 33342 (Invitrogen H3570), and gently homogenized on ice with the pestle 10-15 times. The homogenate was transferred to pre-chilled microcentrifuge tubes and centrifuged at 1000 rcf for 8 min at 4oC to pellet the nuclei. The pellet was resuspended in 1 mL ice-cold NIM buffer, and again centrifuged at 1000 rcf for 8 min at 4oC. The pellet was then resuspended in 450 μL of ice-cold NSB buffer (0.25M sucrose, 5mM MgCl2, 10mM Tris-HCl (pH7.4), 1mM DTT, 9ul of Protease inhibitor), and filtered through 40μM cell strainer. The filtered nuclei suspension was incubated on ice for at least 30 minutes with 50μl of nuclease-free BSA for at least 10 minutes, then incubated with GFP antibody, Alexa Fluor 488 (Invitrogen, A-21311) and anti-NeuN antibody (EMD Millipore MAB377) conjugated with Alexa Fluor 647 (Invitrogen A20173). GFP+/NeuN+ single nuclei were isolated using fluorescence-activated nuclei sorting (FANS) on a BD Influx sorter with 100μm nozzle, and sorted into 384-well plates preloaded with 2μl of digestion buffer for snmC-seq (20 mL digestion buffer consists of 10 mL M-digestion buffer (2×, Zymo D5021-9), 1 ml Proteinase K (20 mg, Zymo D3001-2-20), 9 mL water, and 10 µL unmethylated lambda DNA (100 pg/µL, Promega, D1521)). The collected plates were incubated at 50oC for 20 minutes then stored at -20 oC.
The bisulfite conversion and library preparation were performed following the detailed snmC-seq2 protocol as previously described (Luo, C. et al., Nat. Commun. 2018, 9(1):3824.) or snmC-seq3 protocol described in (Liu, H. et al., Biorxiv. 2023:2023-04.). The libraries were sequenced on Illumina Novaseq 6000 using the S4 flow cell 2 x 150 bp mode.

treatment_protocol: To label neurons projecting to regions of interest, injections of rAAV2-retro-Cre (produced by Salk Vector Core or Vigene, 2x1012 to 1x1013 viral genomes/ml, produced with capsid from Addgene plasmid #81070 packaging pAAV-EF1a-Cre from Addgene plasmid #55636) were made into both hemispheres of the INTACT mice. Animals were anesthetized with either ketamine/xylazine or isoflurane, placed in a stereotaxic frame, and 0.1 to 0.5 microliters of AAV was injected by pressure into stereotaxic coordinates corresponding to the desired projection target.

value: brain

tag: source

value: P

tag: target

value: CBX

tag: Sex

value: male


title: Pool154_Plate6-1-M1-O13


title: Pool154_Plate6-1-M1-N2

value: female


title: KFT, replicate 1, 10x multiome RNA-Seq

description: 10x Genomics


title: Pool154_Plate6-1-M1-N14


title: Pool154_Plate6-1-M1-N13


title: Pool154_Plate6-1-M1-N1


title: Pool154_Plate6-1-M1-M14


title: Pool154_Plate6-1-M1-M1


title: Pool154_Plate6-1-M1-L2


title: Pool154_Plate6-1-M1-L1


title: Pool154_Plate6-1-M1-M2


title: Pool154_Plate6-1-M1-K2


title: Pool154_Plate6-1-M1-I2


title: Pool154_Plate6-1-M1-J14


title: Pool154_Plate6-1-M1-K1


title: Pool154_Plate6-1-M1-H1


title: Pool154_Plate6-1-M1-J2


title: Stomach, control_1


title: Pool154_Plate6-1-M1-J13


title: Pool154_Plate6-1-M1-H13


title: Pool154_Plate6-1-M1-G2


title: Scr1-CTCF

status: Public on May 05 2023

city: Philadelphia

first: Daniel

middle: Sungjoo

last: Park

state: PA

address: 415 Curie Blvd

department: Genetics

institute: University of Pennsylvania

description:
Scr1-CTCF.subtract.bw
Scr1-CTCF.bed
CTCF_Scr.bw

data_processing:
ChIP-seq:Sequencing quality was examined using FastQC (v0.11.9) to ensure that the library GC% and duplication rate were within expected range and adapters were effectively trimmed. Trimming was performed with Trimmomatic (v0.38) using a sliding window of four bases and a minimum average quality score of 20; reads shorter than 20 base pairs were discarded. Remaining reads were aligned against the GRCh38 reference genome using Bowtie2 (v2.4.1) allowing soft clipping ("--local") with the "sensitive local" preset options. Alignments were then filtered for duplicates using the Picard (v2.27.1) "MarkDuplicates" tool. Alignments that mapped to ENCODE blacklisted regions were also removed for downstream analysis using BEDtools v2.30.0.
ChIP-seq:Visualization tracks were generated using the deepTools (v3.3.1) "bamCompare" utility, in which  corresponding input controls were subtracted from IP and reads with a MAPQ score lower than 20 were discarded. Peaks were called for IP libraries against their corresponding input controls using MACS2 (v2.1.1), with default parameters and a 0.01 q-value cutoff. Lost, retained, and gained sites in GSK3A KD were determined with DiffBind v3.6.2 using the DESeq2 analysis method, a false-discovery cutoff of 0.1, and only reads with a minimum MAPQ score of 20. Example ChIP-seq tracks were generated in PyGenomeTracks v3.6.
Hi-C:Raw reads for each Hi-C sample were processed with HiC-Pro (version 2.11.1) to obtain putative interactions with default parameters except LIGATION_SITE = GATCGATC and GENOME_FRAGMENT generated for MboI restriction enzyme. For downstream analyses, ValidPairs were converted to hic files using the “hicpro2juicebox.sh” in utils of HiC-Pro. We recovered the following number of contacts in each condition: Control (507359205), GSK3A KD (445835605).
Assembly: hg38
Supplementary files format and content: ChIP-seq: Bigwig files were generated using BEDtools (v2.27.1) on each biological replicate; bed files of peaks for each biological replicate were called with MACS2 (v2.1.1), with default parameters and a 0.01 q-value cutoff
Supplementary files format and content: Hi-C: .hic files for each condition were generated using HiC-Pro (version 2.11.1) and converted to .cool files using Cooltools

organism: Homo sapiens

growth_protocol: We obtained the HCT-116 cell line from ATCC (RRID:CVCL_0291). All cells were cultured in McCoy’s 5A medium supplemented with 10% fetal bovine serum, 2 mM l-glutamine, 100 U ml−1 penicillin, and 100 μg ml−1 streptomycin at 37 °C with 5% CO2.

extract_protocol:
ChIP-seq: ChIP was performed based on (Shah et al., Dev Cell 2021). HCT-116 cells were cross-linked in culture medium by addition of methanol-free formaldehyde (ThermoFisher, final 1% v/v) and incubated at room temperature for 10 minutes with gentle nutation. Crosslinking was quenched by addition of glycine (final 125 mM) and incubated at room temperature for 5 minutes with gentle nutation. Media was aspirated and replaced with cold DPBS. Cells were scraped and transferred to conical tubes, then pelleted by centrifugation (1500 rpm, 3 minutes, room temperature). Pellets were flash frozen on liquid nitrogen and stored at -80C.  For ChIP, 30 uL protein G magnetic beads (per sample; ThermoFisher) were washed three times in blocking solution (0.5% BSA in DPBS). Beads were then resuspended in 250 uL blocking solution and 2 ug antibody (RAD21, Abcam ab992; CTCF, Cell Signaling Technology 3418) was added. Beads and antibody were rotated at 4C for at least six hours. Nuclei were isolated from frozen cell pellets as follows: pellet was resuspended in 10 mL cold lysis buffer 1 (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% Glycerol, 0.5% NP-40,  0.25% Triton X-100, and protease inhibitors) and rotated at 4C for 10 minutes, followed by centrifugation (1500 rpm, 3 minutes, 4C). Supernatant was aspirated and the pellet was resuspended in 10 mL cold lysis buffer 2 (10mM Tris-HCl pH 8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, and protease inhibitors) and rotated at room temperature for 10 minutes, followed by centrifugation (1500 rpm, 3 minutes, 4C). Supernatant was discarded and nuclei were resuspended in 1 mL cold lysis buffer 3 (10mM Tris-HCl, pH 8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% Na-Deoxycholate, and protease inhibitors) and transferred to pre-chilled 1mL Covaris AFA tubes (Covaris). Nuclei were sonicated using a Covaris S220 sonicator (high cell chromatin shearing for 15 minutes; Covaris). Sonicated chromatin was transferred to 1.5mL microcentrifuge tubes and Triton-X 100 was added (1% final v/v) followed by centrifugation (top speed, 10 minutes, 4C). Supernatant was transferred to a new tube. Antibody-conjugated protein G beads were washed three times in blocking solution, resuspended in 50 ul blocking buffer, and added to 500 ug sonicated chromatin. Chromatin was rotated overnight at 4C. 50 ug lysate was reserved in a separate tube at -20C for input.   On day 2, beads were washed five times in 1 mL RIPA buffer (50mM HEPES-KOH pH 7.5, 500mM LiCl, 1mM EDTA, 1% NP-40, 0.7% Na-Deoxycholate). Beads were then washed in 1 mL final wash buffer (1xTE, 50mM NaCl) for 2 minutes. Beads were finally resuspended in 210 uL elution buffer (50mM Tris-HCl pH 8.0, 10mM EDTA, 1% SDS), and chromatin was eluted from beads by agitation at 65 °C for 30 minutes. 200 uL eluate was transferred to a new tube, and all samples (ChIP and input) were reverse cross-linked overnight at 65 °C with agitation for between 12 and 18 hours. 200 uL 1xTE was added to all samples, and samples were treated with RNase A (final 0.2mg/mL RNase; 37 °C for 2 hours) and proteinase K (final 0.2mg/mL Proteinase K; 55C for 2 hours). DNA was purified using phenol:chloroform extraction and resuspension in 10mM Tris-HCl pH 8.0.  In situ Hi-C: Hi-C libraries were generated from 10e6 cells using the Arima-HiC+ kit (Arima Genomics) according to manufacturer's instructions
ChIP-seq: ChIP and input DNA were quantified by Qubit (ThermoFisher) before library preparation using the NEBNext Ultra II DNA library prep kit (NEB). Samples were indexed for either single or dual-index sequencing. Library quality was assessed on Bioanalyzer (Agilent) and quantified by qPCR (Kapa Biosystems).   In situ Hi-C: Libraries were prepared using the Kapa Hyper Prep Kit with KAPA Library Amplification Primer Mix (KK8502), according to manufacturer’s recommendations. Libraries were validated for quality and size distribution using the Qubit dsDNA HS Assay Kit (Invitrogen, cat# Q32851), KAPA Library Quantification kit (Roche, Cat# KK4824) and TapeStation 2200 (Agilent).
ChIP-seq: Quantified libraries were sequenced via Illumina sequencing using the NextSeq500 (single end, 75bp) and NovaSeq6000 (paired-end, 100bp) platforms. Data from the same libraries were merged for final analysis.  In situ Hi-C: Paired-end sequenced (61bp+61bp) on a NovaSeq 6000 (Illumina).

treatment_protocol: RNAiMAX transfection reagent (Thermo Fisher Scientific) was diluted in Opti-MEM reduced serum medium (Thermo Fisher Scientific), and pipetted onto siRNA. Plates were incubated at room temperature for 20 minutes. HCT-116 cells were trypsinized and resuspended in antibiotic-free medium, then plated on siRNA duplexes to obtain a final siRNA concentration of 25 nM. After 72 h of RNAi treatment, cells were collected for experiments.

tag: cell line

value: wild type

value: control siRNA


title: Pool154_Plate6-1-M1-H14


title: Pool154_Plate6-1-M1-O1


title: KPT, replicate 1, 10x multiome ATAC-Seq

description: 10x Genomics


title: Pool154_Plate6-1-M1-F14


title: Pool154_Plate6-1-M1-F13


title: Pool154_Plate6-1-M1-C2


title: Pool154_Plate6-1-M1-C1


title: Pool154_Plate6-1-M1-F2


title: Pool154_Plate6-1-M1-F1


title: Pool154_Plate6-1-M1-D1


title: Pool154_Plate6-1-M1-D13


title: Pool154_Plate6-1-M1-E1


title: Pool154_Plate6-1-M1-B2


title: Pool154_Plate6-1-M1-B14


title: Pool154_Plate6-1-M1-B1


title: Pool154_Plate6-1-M1-A2


title: Pool154_Plate5-6-I1-P23

value: ENT

value: MOp


title: Pool154_Plate6-1-M1-A1


title: Pool154_Plate6-1-M1-B13


title: Pool154_Plate5-6-I1-O12


title: Pool154_Plate5-6-I1-O23


title: Pool154_Plate5-6-I1-P11


title: Pool154_Plate5-6-I1-O11


title: Pool154_Plate5-6-I1-M24


title: Pool154_Plate5-6-I1-N12


title: Pool154_Plate5-6-I1-N11


title: Pool154_Plate5-6-I1-M12


title: Pool154_Plate5-6-I1-L24


title: Pool154_Plate5-6-I1-N23


title: Pool154_Plate5-6-I1-L23


title: Pool154_Plate5-6-I1-L12


title: Pool154_Plate5-6-I1-L11


title: Pool154_Plate5-6-I1-J23


title: Pool154_Plate5-6-I1-J24


title: Pool154_Plate5-6-I1-K11


title: Pool154_Plate5-6-I1-J12


title: Pool154_Plate5-6-I1-I24


title: Pool154_Plate5-6-I1-K23


title: Pool154_Plate5-6-I1-J11


title: Pool154_Plate5-6-I1-I23


title: Pool154_Plate5-6-I1-I12


title: Pool154_Plate5-6-I1-I11


title: Pool154_Plate5-6-I1-K24


title: Pool154_Plate5-6-I1-P12


title: Pool154_Plate5-6-I1-H12


title: Pool154_Plate5-6-I1-N24


title: Pool154_Plate5-6-I1-H11


title: Pool154_Plate5-6-I1-M11


title: Pool154_Plate5-6-I1-G24


title: Pool154_Plate5-6-I1-F24


title: Pool154_Plate5-6-I1-G23


title: Pool154_Plate5-6-I1-F23


title: Pool154_Plate5-6-I1-E23


title: Pool154_Plate5-6-I1-F12


title: Pool154_Plate5-6-I1-E11


title: Pool154_Plate5-6-I1-D24


title: Pool154_Plate5-6-I1-D12


title: Pool154_Plate5-6-I1-D23


title: Pool154_Plate5-6-I1-C12


title: Pool154_Plate5-6-I1-C23


title: Pool154_Plate5-6-I1-B23


title: Pool154_Plate5-6-I1-C11


title: Pool154_Plate5-6-I1-H23


title: Pool154_Plate5-6-I1-A24


title: Pool154_Plate5-6-I1-B12


title: Pool154_Plate5-5-I1-P22


title: Pool154_Plate5-6-I1-A11


title: Pool154_Plate5-5-I1-P21


title: Pool154_Plate5-6-I1-A12


title: Pool154_Plate5-5-I1-O10


title: Pool154_Plate5-5-I1-O21


title: Pool154_Plate5-5-I1-O9


title: Pool154_Plate5-6-I1-E12


title: Pool154_Plate5-5-I1-O22


title: Pool154_Plate5-5-I1-N22


title: Pool154_Plate5-5-I1-L9


title: Pool154_Plate5-5-I1-M10


title: Pool154_Plate5-5-I1-M21


title: Pool154_Plate5-5-I1-M9


title: Pool154_Plate5-5-I1-M22


title: Pool154_Plate5-5-I1-L21


title: Pool154_Plate5-5-I1-K22


title: Pool154_Plate5-5-I1-K9


title: Pool154_Plate5-5-I1-L10


title: Pool154_Plate5-5-I1-K10


title: Pool154_Plate5-5-I1-J9


title: Pool154_Plate5-5-I1-J22


title: Pool154_Plate5-5-I1-J21


title: Pool154_Plate5-5-I1-J10


title: Pool154_Plate5-5-I1-I22


title: Pool154_Plate5-5-I1-I21


title: Pool154_Plate5-5-I1-H9


title: Pool154_Plate5-5-I1-H22


title: Pool154_Plate5-5-I1-H21


title: Pool154_Plate5-5-I1-G21


title: Pool154_Plate5-5-I1-G9


title: Pool154_Plate5-5-I1-E9


title: Pool154_Plate5-5-I1-G22


title: Pool154_Plate5-5-I1-F10


title: Pool154_Plate5-5-I1-F22


title: Pool154_Plate5-5-I1-K21


title: Pool154_Plate5-5-I1-E21


title: Pool154_Plate5-5-I1-D22


title: Pool154_Plate5-5-I1-C9


title: Pool154_Plate5-5-I1-D10


title: Pool154_Plate5-5-I1-D9


title: Pool154_Plate5-5-I1-B10


title: Pool154_Plate5-5-I1-C10


title: Pool154_Plate5-4-I1-P8


title: Pool154_Plate5-5-I1-A21


title: Pool154_Plate5-5-I1-A22


title: Pool154_Plate5-5-I1-C21


title: Pool154_Plate5-4-I1-P7


title: Pool154_Plate5-5-I1-A10


title: Pool154_Plate5-4-I1-P20


title: Pool154_Plate5-4-I1-O20


title: Pool154_Plate5-4-I1-P19


title: Pool154_Plate5-4-I1-O8


title: Pool154_Plate5-4-I1-O19


title: Pool154_Plate5-4-I1-N8


title: Pool154_Plate5-4-I1-N7


title: Pool154_Plate5-5-I1-E22


title: Pool154_Plate5-4-I1-M19


title: Pool154_Plate5-4-I1-M20


title: Pool154_Plate5-4-I1-M7


title: Pool154_Plate5-4-I1-L20


title: Pool154_Plate5-4-I1-L8


title: Pool154_Plate5-4-I1-N20


title: Pool154_Plate5-4-I1-K7


title: Pool154_Plate5-4-I1-J8


title: Pool154_Plate5-4-I1-I8


title: Pool154_Plate5-4-I1-J20


title: Pool154_Plate5-4-I1-H8


title: Pool154_Plate5-4-I1-I7


title: Pool154_Plate5-4-I1-J19


title: Pool154_Plate5-4-I1-I20


title: Pool154_Plate5-4-I1-I19


title: Pool154_Plate5-4-I1-J7


title: Pool154_Plate5-4-I1-H7


title: Pool154_Plate5-4-I1-G20


title: Pool154_Plate5-4-I1-H19


title: Pool154_Plate5-4-I1-G8


title: Pool154_Plate5-4-I1-G7


title: Pool154_Plate5-4-I1-F8


title: Pool154_Plate5-4-I1-G19


title: Pool154_Plate5-4-I1-F7


title: Pool154_Plate5-4-I1-F20


title: Pool154_Plate5-4-I1-F19


title: Pool154_Plate5-4-I1-E7


title: Pool154_Plate5-4-I1-E20


title: Pool154_Plate5-4-I1-D20


title: Pool154_Plate5-4-I1-C7


title: Pool154_Plate5-4-I1-K19


title: Pool154_Plate5-4-I1-D7


title: Pool154_Plate5-4-I1-C20


title: Pool154_Plate5-4-I1-C19


title: Pool154_Plate5-4-I1-C8


title: Pool154_Plate5-4-I1-B20


title: Pool154_Plate5-3-I1-O6


title: Pool154_Plate5-4-I1-A20


title: Pool154_Plate5-3-I1-P18


title: Pool154_Plate5-3-I1-O5


title: Pool154_Plate5-4-I1-A19


title: Pool154_Plate5-3-I1-P5


title: Pool154_Plate5-3-I1-M5


title: Pool154_Plate5-3-I1-P17


title: Pool154_Plate5-3-I1-P6


title: Pool154_Plate5-3-I1-N5


title: Pool154_Plate5-3-I1-O18


title: Pool154_Plate5-3-I1-O17


title: Pool154_Plate5-3-I1-L6


title: Pool154_Plate5-3-I1-M6


title: Pool154_Plate5-4-I1-E19


title: Pool154_Plate5-3-I1-M18


title: Pool154_Plate5-4-I1-E8


title: Pool154_Plate5-3-I1-M17


title: Pool154_Plate5-3-I1-L17


title: Pool154_Plate5-3-I1-K5


title: Pool154_Plate5-3-I1-K18


title: Pool154_Plate5-3-I1-K6


title: Pool154_Plate5-3-I1-J6


title: Pool154_Plate5-3-I1-K17


title: Pool154_Plate5-3-I1-I5


title: Pool154_Plate5-3-I1-I18


title: Pool154_Plate5-3-I1-N6


title: Pool154_Plate5-3-I1-I17


title: Pool154_Plate5-3-I1-H6


title: Pool154_Plate5-3-I1-G18


title: Pool154_Plate5-3-I1-F5


title: Pool154_Plate5-3-I1-E17


title: Pool154_Plate5-3-I1-E18


title: Pool154_Plate5-3-I1-D18


title: Pool154_Plate5-3-I1-J18


title: Pool154_Plate5-3-I1-J5


title: Pool154_Plate5-3-I1-E5


title: Pool154_Plate5-3-I1-C17


title: Pool154_Plate5-3-I1-B6


title: Pool154_Plate5-3-I1-D6


title: Pool154_Plate5-3-I1-A6


title: Pool154_Plate5-3-I1-B18


title: Pool154_Plate5-3-I1-A5


title: Pool154_Plate5-3-I1-B5


title: Pool154_Plate5-3-I1-A18


title: Pool154_Plate5-3-I1-A17


title: Pool154_Plate5-2-I1-P15


title: Pool154_Plate5-2-I1-P16


title: Pool154_Plate5-3-I1-H18


title: Pool154_Plate5-2-I1-P3


title: Pool154_Plate5-3-I1-H17


title: Pool154_Plate5-3-I1-F18


title: Pool154_Plate5-3-I1-E6


title: Pool154_Plate5-3-I1-G5


title: Pool154_Plate5-2-I1-O16


title: Pool154_Plate5-3-I1-F17


title: Pool154_Plate5-3-I1-C5


title: Pool154_Plate5-2-I1-O15


title: Pool154_Plate5-2-I1-N4


title: Pool154_Plate5-2-I1-N15


title: Pool154_Plate5-2-I1-L3


title: Pool154_Plate5-2-I1-M3


title: Pool154_Plate5-2-I1-M16


title: Pool154_Plate5-2-I1-L15


title: Pool154_Plate5-2-I1-M4


title: Pool154_Plate5-2-I1-M15


title: Pool154_Plate5-2-I1-K3


title: Pool154_Plate5-2-I1-J16


title: Pool154_Plate5-2-I1-I16


title: Pool154_Plate5-2-I1-I3


title: Pool154_Plate5-2-I1-L16


title: Pool154_Plate5-2-I1-H4


title: Pool154_Plate5-2-I1-K4


title: Pool154_Plate5-2-I1-J15


title: Pool154_Plate5-2-I1-G15


title: Pool154_Plate5-2-I1-G4


title: Pool154_Plate5-2-I1-F16


title: Pool154_Plate5-2-I1-E15


title: Pool154_Plate5-2-I1-N16


title: Pool154_Plate5-2-I1-E3


title: Pool154_Plate5-2-I1-D3


title: Pool154_Plate5-2-I1-H16


title: Pool154_Plate5-2-I1-F3


title: Pool154_Plate5-2-I1-D4


title: Pool154_Plate5-2-I1-C4


title: Pool154_Plate5-2-I1-C16


title: Pool154_Plate5-2-I1-D15


title: Pool154_Plate5-2-I1-E4


title: Pool154_Plate5-2-I1-H15


title: Pool154_Plate5-2-I1-I4


title: Pool154_Plate5-2-I1-C3


title: Pool154_Plate5-2-I1-A4


title: Pool154_Plate5-2-I1-B3


title: Pool154_Plate5-2-I1-A16


title: Pool154_Plate5-1-I1-P14


title: Pool154_Plate5-2-I1-A15


title: Pool154_Plate5-1-I1-P13


title: Pool154_Plate5-1-I1-P2


title: Pool154_Plate5-1-I1-O1


title: Pool154_Plate5-1-I1-O13


title: Pool154_Plate5-1-I1-O14


title: Pool154_Plate5-2-I1-A3


title: Pool154_Plate5-1-I1-O2


title: Pool154_Plate5-2-I1-D16


title: Pool154_Plate5-1-I1-N13


title: Pool154_Plate5-1-I1-M2


title: Pool154_Plate5-1-I1-M14


title: Pool154_Plate5-1-I1-M13


title: Pool154_Plate5-1-I1-L14


title: Pool154_Plate5-1-I1-M1


title: Pool154_Plate5-2-I1-B4


title: Pool154_Plate5-1-I1-L1


title: Pool154_Plate5-1-I1-P1


title: Pool154_Plate5-2-I1-B16


title: Pool154_Plate5-1-I1-K14


title: Pool154_Plate5-1-I1-K1


title: Pool154_Plate5-1-I1-N2


title: Pool154_Plate5-1-I1-K2


title: Pool154_Plate5-1-I1-J14


title: Pool154_Plate5-1-I1-N14


title: Pool154_Plate5-1-I1-J2


title: Pool154_Plate5-3-I1-C6


title: Pool154_Plate5-1-I1-J13


title: Pool154_Plate5-1-I1-I14


title: Pool154_Plate5-1-I1-H13


title: Pool154_Plate5-1-I1-H1


title: Pool154_Plate5-1-I1-I13


title: Pool154_Plate5-1-I1-I1


title: Pool154_Plate5-1-I1-G1


title: Pool154_Plate5-1-I1-F14


title: Pool154_Plate5-1-I1-G14


title: Pool154_Plate5-1-I1-E14


title: Pool154_Plate5-1-I1-E13


title: Pool154_Plate5-1-I1-E1


title: Pool154_Plate5-1-I1-D1


title: Pool154_Plate5-1-I1-C2


title: Pool154_Plate5-1-I1-D14


title: Pool154_Plate5-1-I1-C13


title: Pool154_Plate5-1-I1-C14


title: Pool154_Plate5-1-I1-C1


title: Pool154_Plate5-1-I1-B14


title: Pool154_Plate5-1-I1-B13


title: Pool154_Plate5-1-I1-A2


title: Pool154_Plate5-1-I1-G13


title: Pool154_Plate5-1-I1-A14


title: Pool154_Plate5-1-I1-A13


title: Pool154_Plate5-1-I1-A1


title: Pool154_Plate4-6-G1-P24

value: PAG

value: MOB


title: Pool154_Plate5-1-I1-D2


title: Pool154_Plate4-6-G1-O23


title: Pool154_Plate5-1-I1-D13


title: Pool154_Plate4-6-G1-P12


title: Pool154_Plate4-6-G1-O24


title: Pool154_Plate4-6-G1-P23


title: Pool154_Plate4-6-G1-L24


title: Pool154_Plate4-6-G1-M12


title: Pool154_Plate4-6-G1-N23


title: Pool154_Plate4-6-G1-M24


title: Pool154_Plate4-6-G1-N24


title: Pool154_Plate4-6-G1-N11


title: Pool154_Plate4-6-G1-M23


title: Pool154_Plate4-6-G1-L23


title: Pool154_Plate4-6-G1-K24


title: Pool154_Plate4-6-G1-K11


title: Pool154_Plate4-6-G1-K23


title: Pool154_Plate4-6-G1-L12


title: Pool154_Plate4-6-G1-L11


title: Pool154_Plate4-6-G1-J24


title: Pool154_Plate4-6-G1-J23


title: Pool154_Plate4-6-G1-I24


title: Pool154_Plate4-6-G1-J12


title: Pool154_Plate4-6-G1-I12


title: Pool154_Plate4-6-G1-I11


title: Pool154_Plate5-1-I1-B1


title: Pool154_Plate4-6-G1-G23


title: Pool154_Plate4-6-G1-H23


title: Pool154_Plate4-6-G1-H12


title: Pool154_Plate4-6-G1-G11


title: Pool154_Plate4-6-G1-G12


title: Pool154_Plate4-6-G1-G24


title: Pool154_Plate4-6-G1-H24


title: Pool154_Plate4-6-G1-F23


title: Pool154_Plate4-6-G1-F11


title: Pool154_Plate4-6-G1-E23


title: Pool154_Plate4-6-G1-E11


title: Pool154_Plate4-6-G1-F12


title: Pool154_Plate4-6-G1-D23


title: Pool154_Plate4-6-G1-D24


title: Pool154_Plate4-6-G1-D12


title: Pool154_Plate4-6-G1-C23


title: Pool154_Plate4-6-G1-B24


title: Pool154_Plate4-6-G1-B11


title: Pool154_Plate4-6-G1-B23


title: Pool154_Plate4-6-G1-B12


title: Pool154_Plate4-6-G1-A24


title: Pool154_Plate4-6-G1-F24


title: Pool154_Plate4-6-G1-A23


title: Pool154_Plate4-6-G1-A12


title: Pool154_Plate4-6-G1-D11


title: Pool154_Plate4-6-G1-A11


title: Pool154_Plate4-5-G1-P9


title: Pool154_Plate4-5-G1-P10


title: Pool154_Plate4-5-G1-O9


title: Pool154_Plate4-5-G1-O22


title: Pool154_Plate4-5-G1-O21


title: Pool154_Plate4-6-G1-C11


title: Pool154_Plate4-5-G1-N9


title: Pool154_Plate4-5-G1-N22


title: Pool154_Plate4-5-G1-P21


title: Pool154_Plate4-5-G1-M22


title: Pool154_Plate4-5-G1-N10


title: Pool154_Plate4-5-G1-M9


title: Pool154_Plate4-5-G1-N21


title: Pool154_Plate4-5-G1-M21


title: Pool154_Plate4-5-G1-L22


title: Pool154_Plate4-5-G1-P22


title: Pool154_Plate4-5-G1-L9


title: Pool154_Plate5-2-I1-H3


title: Pool154_Plate4-5-G1-K9


title: Pool154_Plate5-1-I1-J1


title: Pool154_Plate4-5-G1-K10


title: Pool154_Plate4-5-G1-J22


title: Pool154_Plate4-5-G1-K21


title: Pool154_Plate4-5-G1-J21


title: Pool154_Plate4-5-G1-I9


title: Pool154_Plate4-5-G1-I22


title: Pool154_Plate4-5-G1-H22


title: Pool154_Plate4-5-G1-H21


title: Pool154_Plate4-5-G1-H9


title: Pool154_Plate4-5-G1-I10


title: Pool154_Plate4-5-G1-K22


title: Pool154_Plate4-5-G1-H10


title: Pool154_Plate4-5-G1-I21


title: Pool154_Plate4-6-G1-I23


title: Pool154_Plate4-5-G1-F9


title: Pool154_Plate4-5-G1-F22


title: Pool154_Plate4-5-G1-G22


title: Pool154_Plate4-5-G1-F21


title: Pool154_Plate4-5-G1-F10


title: Pool154_Plate4-5-G1-E22


title: Pool154_Plate4-5-G1-G21


title: Pool154_Plate4-6-G1-C24


title: Pool154_Plate4-5-G1-E21


title: Pool154_Plate4-5-G1-D22


title: Pool154_Plate4-5-G1-E10


title: Pool154_Plate4-5-G1-D10


title: Pool154_Plate4-5-G1-C22


title: Pool154_Plate4-5-G1-D21


title: Pool154_Plate4-5-G1-J10


title: Pool154_Plate4-5-G1-C21


title: Pool154_Plate4-5-G1-B9


title: Pool154_Plate4-5-G1-G9


title: Pool154_Plate4-5-G1-A9


title: Pool154_Plate4-5-G1-B10


title: Pool154_Plate4-5-G1-B21


title: Pool154_Plate4-5-G1-A21


title: Pool154_Plate4-5-G1-A22


title: Pool154_Plate4-4-G1-P20


title: Pool154_Plate4-4-G1-P8


title: Pool154_Plate4-4-G1-O19


title: Pool154_Plate4-5-G1-D9


title: Pool154_Plate4-4-G1-M20


title: Pool154_Plate4-4-G1-N19


title: Pool154_Plate4-4-G1-O20


title: Pool154_Plate4-4-G1-N8


title: Pool154_Plate4-4-G1-L20


title: Pool154_Plate4-4-G1-L7


title: Pool154_Plate4-4-G1-K8


title: Pool154_Plate4-5-G1-C9


title: Pool154_Plate4-4-G1-M19


title: Pool154_Plate4-4-G1-L8


title: Pool154_Plate4-4-G1-N20


title: Pool154_Plate4-4-G1-J8


title: Pool154_Plate4-4-G1-K19


title: Pool154_Plate4-4-G1-L19


title: Pool154_Plate4-4-G1-J20


title: Pool154_Plate4-4-G1-J19


title: Pool154_Plate4-4-G1-I20


title: Pool154_Plate4-4-G1-I19


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title: Pool154_Plate4-4-G1-K20


title: Pool154_Plate4-4-G1-H19


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value: MRN


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title: Pool154_Plate3-2-E1-B15


title: Pool154_Plate3-2-E1-A15


title: Pool154_Plate3-1-E1-P14


title: Pool154_Plate3-2-E1-D4


title: Pool154_Plate3-1-E1-N2


title: Pool154_Plate3-1-E1-O2


title: Pool154_Plate3-1-E1-O14


title: Pool154_Plate3-1-E1-N13


title: Pool154_Plate3-1-E1-M14


title: Pool154_Plate3-1-E1-N1


title: Pool154_Plate3-1-E1-M2


title: Pool154_Plate3-2-E1-B4


title: Pool154_Plate3-1-E1-M13


title: Pool154_Plate3-1-E1-L2


title: Pool154_Plate3-1-E1-K14


title: Pool154_Plate3-1-E1-L13


title: Pool154_Plate3-1-E1-L1


title: Pool154_Plate3-1-E1-J1


title: Pool154_Plate3-1-E1-J14


title: Pool154_Plate3-1-E1-K13


title: Pool154_Plate3-1-E1-L14


title: Pool154_Plate3-1-E1-P1


title: Pool154_Plate3-1-E1-I2


title: Pool154_Plate3-1-E1-H1


title: Pool154_Plate3-1-E1-F14


title: Pool154_Plate3-1-E1-H14


title: Pool154_Plate3-1-E1-G13


title: Pool154_Plate3-1-E1-I14


title: Pool154_Plate3-1-E1-G2


title: Pool154_Plate3-1-E1-G1


title: Pool154_Plate3-1-E1-F1


title: Pool154_Plate3-1-E1-H2


title: Pool154_Plate3-1-E1-F13


title: Pool154_Plate3-1-E1-E2


title: Pool154_Plate3-1-E1-E14


title: Pool154_Plate3-1-E1-D2


title: Pool154_Plate3-1-E1-B13


title: Pool154_Plate3-1-E1-C2


title: Pool154_Plate3-1-E1-D1


title: Pool154_Plate3-1-E1-B2


title: Pool154_Plate3-1-E1-B14


title: Pool154_Plate3-1-E1-B1


title: Pool154_Plate3-1-E1-A2


title: Pool154_Plate2-6-C1-O24

value: HY


title: Pool154_Plate2-6-C1-O23


title: Pool154_Plate3-1-E1-G14


title: Pool154_Plate2-6-C1-O12


title: Pool154_Plate3-1-E1-A14


title: Pool154_Plate2-6-C1-O11


title: Pool154_Plate2-6-C1-N24


title: Pool154_Plate2-6-C1-N11


title: Pool154_Plate2-6-C1-M23


title: Pool154_Plate2-6-C1-K24


title: Pool154_Plate2-6-C1-L24


title: Pool154_Plate2-6-C1-M24


title: Pool154_Plate2-6-C1-K12


title: Pool154_Plate3-1-E1-E1


title: Pool154_Plate2-6-C1-L23


title: Pool154_Plate2-6-C1-J23


title: Pool154_Plate2-6-C1-J24


title: Pool154_Plate2-6-C1-J12


title: Pool154_Plate2-6-C1-I24


title: Pool154_Plate2-6-C1-I23


title: Pool154_Plate2-6-C1-I12


title: Pool154_Plate2-6-C1-I11


title: Pool154_Plate2-6-C1-H23


title: Pool154_Plate2-6-C1-H24


title: Pool154_Plate2-6-C1-H12


title: Pool154_Plate2-6-C1-H11


title: Pool154_Plate2-6-C1-F12


title: Pool154_Plate2-6-C1-M12


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title: Pool154_Plate2-6-C1-E24


title: Pool154_Plate2-6-C1-F11


title: Pool154_Plate2-6-C1-E23


title: Pool154_Plate2-6-C1-K23


title: Pool154_Plate2-6-C1-E11


title: Pool154_Plate2-6-C1-D24


title: Pool154_Plate2-6-C1-E12


title: Pool154_Plate2-6-C1-C24


title: Pool154_Plate2-6-C1-F24


title: Pool154_Plate2-6-C1-B23


title: Pool154_Plate2-6-C1-C11


title: Pool154_Plate2-6-C1-D11


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title: Pool154_Plate2-6-C1-K11


title: Pool154_Plate2-6-C1-B11


title: Pool154_Plate2-6-C1-G24


title: Pool154_Plate2-5-C1-O21


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title: Pool154_Plate2-5-C1-H21


title: Pool154_Plate2-5-C1-G22


title: Pool154_Plate2-5-C1-I21


title: Pool154_Plate2-5-C1-G21


title: Pool154_Plate2-5-C1-H22


title: Pool154_Plate2-6-C1-A11


title: Pool154_Plate2-6-C1-A24


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title: Pool154_Plate2-5-C1-F10


title: Pool154_Plate2-5-C1-D22


title: Pool154_Plate2-5-C1-E22


title: Pool154_Plate2-5-C1-E9


title: Pool154_Plate2-5-C1-E21


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title: Pool154_Plate2-5-C1-C21


title: Pool154_Plate2-5-C1-F21


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title: Pool154_Plate2-5-C1-B10


title: Pool154_Plate2-5-C1-B21


title: Pool154_Plate2-5-C1-A22


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title: Pool154_Plate2-4-C1-F19


title: Pool154_Plate2-4-C1-F7


title: Pool154_Plate2-4-C1-E7


title: Pool154_Plate2-4-C1-D20


title: Pool154_Plate2-4-C1-D19


title: Pool154_Plate2-4-C1-D7


title: Pool154_Plate2-4-C1-E19


title: Pool154_Plate2-4-C1-C8


title: Pool154_Plate2-4-C1-H19


title: Pool154_Plate2-4-C1-A7


title: Pool154_Plate2-4-C1-C19


title: Pool154_Plate2-4-C1-A8


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title: Pool154_Plate2-4-C1-A20


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title: Pool154_Plate2-3-C1-P17


title: Pool154_Plate2-3-C1-P18


title: Pool154_Plate2-4-C1-A19


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title: Pool154_Plate2-3-C1-N6


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title: Pool154_Plate2-3-C1-I5


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title: Pool154_Plate2-1-C1-H14


title: Pool154_Plate2-1-C1-I14


title: Pool154_Plate2-1-C1-E14


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title: Pool154_Plate2-1-C1-F14


title: Pool154_Plate2-2-C1-C3


title: Pool154_Plate2-2-C1-A15


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title: Pool154_Plate2-1-C1-D13


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title: Pool154_Plate2-1-C1-B13


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value: AMY


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title: Pool154_Plate1-3-A1-F5


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title: Pool154_Plate1-3-A1-E5


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title: Pool154_Plate1-2-A1-J4


title: Pool154_Plate1-2-A1-M15


title: Pool154_Plate1-2-A1-J3


title: Pool154_Plate1-2-A1-N3


title: Pool154_Plate1-2-A1-L15


title: Pool154_Plate1-2-A1-K3


title: Pool154_Plate1-2-A1-P16


title: Pool154_Plate1-2-A1-M4


title: Pool154_Plate1-2-A1-K16


title: Pool154_Plate1-2-A1-J16


title: Pool154_Plate1-2-A1-L3


title: Pool154_Plate1-2-A1-I16


title: Pool154_Plate1-2-A1-N16


title: Pool154_Plate1-2-A1-H3


title: Pool154_Plate1-2-A1-O4


title: Pool154_Plate1-2-A1-O15


title: Pool154_Plate1-2-A1-P15


title: Pool154_Plate1-2-A1-I3


title: Pool154_Plate1-2-A1-M16


title: Pool154_Plate1-2-A1-I15


title: Pool154_Plate1-2-A1-G16


title: Pool154_Plate1-2-A1-G3


title: Pool154_Plate1-2-A1-L16


title: Pool154_Plate1-2-A1-E16


title: Pool154_Plate1-2-A1-F15


title: Pool154_Plate1-2-A1-E3


title: Pool154_Plate1-2-A1-F16


title: Pool154_Plate1-2-A1-G15


title: Pool154_Plate1-2-A1-F3


title: Pool154_Plate1-2-A1-F4


title: Pool154_Plate1-2-A1-A16


title: Pool154_Plate1-2-A1-D3


title: Pool154_Plate1-2-A1-A3


title: Pool154_Plate1-2-A1-C3


title: Pool154_Plate1-2-A1-B15


title: Pool154_Plate1-2-A1-D15


title: Pool154_Plate1-2-A1-C15


title: Pool154_Plate1-2-A1-D16


title: Pool154_Plate1-2-A1-A4


title: Pool154_Plate1-2-A1-E15


title: Pool154_Plate1-2-A1-B16


title: Pool154_Plate1-2-A1-B4


title: Pool154_Plate1-2-A1-A15


title: Pool154_Plate1-2-A1-D4


title: Pool154_Plate1-1-A1-P2


title: Pool154_Plate1-1-A1-P14


title: Pool154_Plate1-2-A1-C16


title: Pool154_Plate1-1-A1-I13


title: Pool154_Plate1-1-A1-L1


title: Pool154_Plate1-1-A1-K2


title: Pool154_Plate1-1-A1-I2


title: Pool154_Plate1-1-A1-J14


title: Pool154_Plate1-1-A1-L13


title: Pool154_Plate1-1-A1-K14


title: Pool154_Plate1-1-A1-O13


title: Pool154_Plate1-1-A1-P1


title: Pool154_Plate1-1-A1-L14


title: Pool154_Plate1-1-A1-O1


title: Pool154_Plate1-1-A1-N14


title: Pool154_Plate1-1-A1-N1


title: Pool154_Plate1-1-A1-M2


title: Pool154_Plate1-1-A1-P13


title: Pool154_Plate1-1-A1-O14


title: Pool154_Plate1-1-A1-O2


title: Pool154_Plate1-1-A1-J13


title: Pool154_Plate1-1-A1-J2


title: Pool154_Plate1-1-A1-J1


title: Pool154_Plate1-1-A1-H13


title: Pool154_Plate1-1-A1-K13


title: Pool154_Plate1-1-A1-G14


title: Pool154_Plate1-1-A1-H1


title: Pool154_Plate1-1-A1-G2


title: Pool154_Plate1-1-A1-H14


title: Pool154_Plate1-1-A1-N13


title: Pool154_Plate1-1-A1-I14


title: Pool142_Plate4-6-A22-N11

value: PIRp


title: Pool142_Plate4-6-A22-N24


title: Pool142_Plate4-6-A22-O11

value: PIRa


title: Pool142_Plate4-6-A22-O23

value: brain


title: Pool142_Plate4-6-A22-N12


title: Pool154_Plate1-1-A1-A13


title: Pool142_Plate4-6-A22-P23


title: Pool154_Plate1-1-A1-F1


title: Pool154_Plate1-1-A1-A2


title: Pool154_Plate1-1-A1-G13


title: Pool154_Plate1-1-A1-B13


title: Pool154_Plate1-1-A1-E14


title: Pool154_Plate1-1-A1-C2


title: Pool142_Plate4-6-A22-O24


title: Pool154_Plate1-1-A1-F2


title: Pool142_Plate4-6-A22-P11


title: Pool142_Plate4-6-A22-P24


title: Pool154_Plate1-1-A1-D13


title: Pool154_Plate1-1-A1-E2


title: Pool154_Plate1-1-A1-D1


title: Pool154_Plate1-1-A1-C1


title: Pool154_Plate1-1-A1-F14


title: Pool154_Plate1-1-A1-A14


title: Pool142_Plate4-6-A22-K12


title: Pool142_Plate4-6-A22-L12


title: Pool142_Plate4-6-A22-K11


title: Pool142_Plate4-6-A22-J24


title: Pool142_Plate4-6-A22-K23


title: Pool142_Plate4-6-A22-M12


title: Pool142_Plate4-6-A22-L24


title: Pool142_Plate4-6-A22-L23


title: Pool142_Plate4-6-A22-I24


title: Pool142_Plate4-6-A22-G12


title: Pool142_Plate4-6-A22-G23


title: Pool142_Plate4-6-A22-H24


title: Pool142_Plate4-6-A22-I23


title: Pool142_Plate4-6-A22-G24


title: Pool142_Plate4-6-A22-I12


title: Pool142_Plate4-6-A22-D12


title: Pool142_Plate4-6-A22-F24


title: Pool142_Plate4-6-A22-H12


title: Pool142_Plate4-6-A22-F11


title: Pool142_Plate4-6-A22-F23


title: Pool142_Plate4-6-A22-J12


title: Pool142_Plate4-6-A22-C11


title: Pool142_Plate4-6-A22-C23


title: Pool142_Plate4-6-A22-D23


title: Pool142_Plate4-6-A22-D11


title: Pool142_Plate4-6-A22-A23


title: Pool142_Plate4-6-A22-D24


title: Pool142_Plate4-6-A22-B23


title: Pool142_Plate4-6-A22-I11


title: Pool142_Plate4-6-A22-B12


title: Pool142_Plate4-6-A22-E23


title: Pool142_Plate4-6-A22-A12


title: Pool142_Plate4-5-A22-P9


title: Pool142_Plate4-6-A22-E11


title: Pool142_Plate4-6-A22-G11


title: Pool142_Plate4-6-A22-E24


title: Pool142_Plate4-6-A22-C12


title: Pool142_Plate4-5-A22-P21


title: Pool142_Plate4-5-A22-O21


title: Pool142_Plate4-5-A22-N22


title: Pool142_Plate4-5-A22-N21


title: Pool142_Plate4-5-A22-P22


title: Pool142_Plate4-5-A22-N9


title: Pool142_Plate4-5-A22-M10


title: Pool142_Plate4-5-A22-O10


title: Pool142_Plate4-5-A22-P10


title: Pool142_Plate4-5-A22-M22


title: Pool142_Plate4-5-A22-L22


title: Pool142_Plate4-5-A22-K10


title: Pool142_Plate4-5-A22-L9


title: Pool142_Plate4-5-A22-L10


title: Pool142_Plate4-5-A22-L21


title: Pool142_Plate4-5-A22-J21


title: Pool142_Plate4-5-A22-J22


title: Pool142_Plate4-5-A22-K9


title: Pool142_Plate4-5-A22-O9


title: Pool142_Plate4-5-A22-O22


title: Pool142_Plate4-5-A22-M9


title: Pool142_Plate4-5-A22-I22


title: Pool142_Plate4-5-A22-I9


title: Pool142_Plate4-5-A22-I10


title: Pool142_Plate4-5-A22-I21


title: Pool142_Plate4-5-A22-H22


title: Pool142_Plate4-5-A22-N10


title: Pool142_Plate4-5-A22-H9


title: Pool142_Plate4-5-A22-E9


title: Pool142_Plate4-5-A22-H21


title: Pool142_Plate4-5-A22-F9


title: Pool142_Plate4-5-A22-G10


title: Pool142_Plate4-5-A22-H10


title: Pool142_Plate4-5-A22-G21


title: Pool142_Plate4-5-A22-G9


title: Pool142_Plate4-5-A22-F10


title: Pool142_Plate4-5-A22-E10


title: Pool142_Plate4-5-A22-E22


title: Pool142_Plate4-5-A22-F22


title: Pool142_Plate4-5-A22-F21


title: Pool142_Plate4-5-A22-E21


title: Pool142_Plate4-5-A22-D22


title: Pool142_Plate4-5-A22-B22


title: Pool142_Plate4-5-A22-D9


title: Pool142_Plate4-5-A22-C21


title: Pool142_Plate4-5-A22-C22


title: Pool142_Plate4-5-A22-B21


title: Pool142_Plate4-5-A22-A9


title: Pool142_Plate4-5-A22-B10


title: Pool142_Plate4-5-A22-A22


title: Pool142_Plate4-4-A22-P20


title: Pool142_Plate4-4-A22-P7


title: Pool142_Plate4-4-A22-P8


title: Pool142_Plate4-5-A22-A10


title: Pool142_Plate4-5-A22-D21


title: Pool142_Plate4-5-A22-C9


title: Pool142_Plate4-4-A22-O7


title: Pool142_Plate4-4-A22-M7


title: Pool142_Plate4-4-A22-N8


title: Pool142_Plate4-4-A22-N7


title: Pool142_Plate4-4-A22-O19


title: Pool142_Plate4-4-A22-N20


title: Pool142_Plate4-4-A22-N19


title: Pool142_Plate4-4-A22-L8


title: Pool142_Plate4-4-A22-L7


title: Pool142_Plate4-4-A22-L20


title: Pool142_Plate4-4-A22-K8


title: Pool142_Plate4-4-A22-L19


title: Pool142_Plate4-4-A22-O20


title: Pool142_Plate4-4-A22-K7


title: Pool142_Plate4-4-A22-J8


title: Pool142_Plate4-4-A22-P19


title: Pool142_Plate4-4-A22-J20


title: Pool142_Plate4-4-A22-J7


title: Pool142_Plate4-4-A22-M20


title: Pool142_Plate4-5-A22-A21


title: Pool142_Plate4-4-A22-K20


title: Pool142_Plate4-4-A22-H7


title: Pool142_Plate4-4-A22-I19


title: Pool142_Plate4-4-A22-M8


title: Pool142_Plate4-4-A22-H20


title: Pool142_Plate4-4-A22-J19


title: Pool142_Plate4-4-A22-I7


title: Pool142_Plate4-4-A22-I20


title: Pool142_Plate4-4-A22-I8


title: Pool142_Plate4-4-A22-H19


title: Pool142_Plate4-4-A22-G8


title: Pool142_Plate4-4-A22-G19


title: Pool142_Plate4-4-A22-G20


title: Pool142_Plate4-4-A22-F19


title: Pool142_Plate4-4-A22-F20


title: Pool142_Plate4-4-A22-D8


title: Pool142_Plate4-4-A22-D19


title: Pool142_Plate4-4-A22-E19


title: Pool142_Plate4-4-A22-D20


title: Pool142_Plate4-4-A22-E8


title: Pool142_Plate4-4-A22-E20


title: Pool142_Plate4-4-A22-D7


title: Pool142_Plate4-4-A22-G7


title: Pool142_Plate4-4-A22-C7


title: Pool142_Plate4-4-A22-C8


title: Pool142_Plate4-4-A22-E7


title: Pool142_Plate4-4-A22-B7


title: Pool142_Plate4-4-A22-C20


title: Pool142_Plate4-4-A22-C19


title: Pool142_Plate4-4-A22-B19


title: Pool142_Plate4-3-A22-P18


title: Pool142_Plate4-3-A22-P5


title: Pool142_Plate4-3-A22-O5


title: Pool142_Plate4-3-A22-N17


title: Pool142_Plate4-3-A22-M6


title: Pool142_Plate4-3-A22-O18


title: Pool142_Plate4-3-A22-M18


title: Pool142_Plate4-3-A22-O17


title: Pool142_Plate4-3-A22-M17


title: Pool142_Plate4-3-A22-L5


title: Pool142_Plate4-3-A22-L6


title: Pool142_Plate4-3-A22-L17


title: Pool142_Plate4-3-A22-L18


title: Pool142_Plate4-3-A22-J5


title: Pool142_Plate4-3-A22-J17


title: Pool142_Plate4-3-A22-J6


title: Pool142_Plate4-3-A22-J18


title: Pool142_Plate4-3-A22-H18


title: Pool142_Plate4-3-A22-H17


title: Pool142_Plate4-3-A22-K6


title: Pool142_Plate4-3-A22-K17


title: Pool142_Plate4-3-A22-G6


title: Pool142_Plate4-3-A22-F6


title: Pool142_Plate4-3-A22-I17


title: Pool142_Plate4-3-A22-I6


title: Pool142_Plate4-4-A22-A20


title: Pool142_Plate4-3-A22-G18


title: Pool142_Plate4-3-A22-G17


title: Pool142_Plate4-3-A22-E6


title: Pool142_Plate4-3-A22-F18


title: Pool142_Plate4-3-A22-F5


title: Pool142_Plate4-3-A22-E5


title: Pool142_Plate4-3-A22-D17


title: Pool142_Plate4-3-A22-D18


title: Pool142_Plate4-3-A22-D6


title: Pool142_Plate4-3-A22-E17


title: Pool142_Plate4-3-A22-D5


title: Pool142_Plate4-3-A22-K18


title: Pool142_Plate4-3-A22-C5


title: Pool142_Plate4-3-A22-C17


title: Pool142_Plate4-3-A22-B6


title: Pool142_Plate4-3-A22-B18


title: Pool142_Plate4-4-A22-F7


title: Pool142_Plate4-3-A22-B17


title: Pool142_Plate4-2-A22-P3


title: Pool142_Plate4-2-A22-P4


title: Pool142_Plate4-2-A22-P15


title: Pool142_Plate4-2-A22-N16


title: Pool142_Plate4-2-A22-N15


title: Pool142_Plate4-2-A22-N4


title: Pool142_Plate4-2-A22-O3


title: Pool142_Plate4-2-A22-O4


title: Pool142_Plate4-2-A22-M3


title: Pool142_Plate4-2-A22-M16


title: Pool142_Plate4-2-A22-M15


title: Pool142_Plate4-2-A22-L4


title: Pool142_Plate4-2-A22-L16


title: Pool142_Plate4-3-A22-C6


title: Pool142_Plate4-2-A22-J16


title: Pool142_Plate4-2-A22-K15


title: Pool142_Plate4-2-A22-J4


title: Pool142_Plate4-3-A22-A5


title: Pool142_Plate4-2-A22-K3


title: Pool142_Plate4-3-A22-A18


title: Pool142_Plate4-2-A22-P16


title: Pool142_Plate4-2-A22-J15


title: Pool142_Plate4-2-A22-O15


title: Pool142_Plate4-2-A22-I3


title: Pool142_Plate4-2-A22-H15


title: Pool142_Plate4-2-A22-I15


title: Pool142_Plate4-2-A22-F3


title: Pool142_Plate4-2-A22-G16


title: Pool142_Plate4-2-A22-F16


title: Pool142_Plate4-2-A22-F15


title: Pool142_Plate4-2-A22-E4


title: Pool142_Plate4-2-A22-K16


title: Pool142_Plate4-2-A22-E3


title: Pool142_Plate4-2-A22-K4


title: Pool142_Plate4-2-A22-D15


title: Pool142_Plate4-2-A22-D16


title: Pool142_Plate4-2-A22-D3


title: Pool142_Plate4-2-A22-C3


title: Pool142_Plate4-2-A22-B4


title: Pool142_Plate4-2-A22-B15


title: Pool142_Plate4-2-A22-B16


title: Pool142_Plate4-2-A22-A3


title: Pool142_Plate4-2-A22-I4


title: Pool142_Plate4-2-A22-H16


title: Pool142_Plate4-1-A22-P14


title: Pool142_Plate4-1-A22-P2


title: Pool142_Plate4-1-A22-P13


title: Pool142_Plate4-1-A22-P1


title: Pool142_Plate4-1-A22-O2


title: Pool142_Plate4-1-A22-O14


title: Pool142_Plate4-1-A22-N14


title: Pool142_Plate4-1-A22-N2


title: Pool142_Plate4-2-A22-C16


title: Pool142_Plate4-1-A22-N1


title: Pool142_Plate4-1-A22-O13


title: Pool142_Plate4-1-A22-M14


title: Pool142_Plate4-2-A22-A16


title: Pool142_Plate4-1-A22-L14


title: Pool142_Plate4-1-A22-M13


title: Pool142_Plate4-1-A22-L2


title: Pool142_Plate4-1-A22-I14


title: Pool142_Plate4-1-A22-L1


title: Pool142_Plate4-1-A22-K14


title: Pool142_Plate4-1-A22-J13


title: Pool142_Plate4-1-A22-I13


title: Pool142_Plate4-1-A22-J14


title: Pool142_Plate4-1-A22-I1


title: Pool142_Plate4-1-A22-H2


title: Pool142_Plate4-1-A22-H14


title: Pool142_Plate4-1-A22-H1


title: Pool142_Plate4-1-A22-H13


title: Pool142_Plate4-1-A22-F1


title: Pool142_Plate4-1-A22-F14


title: Pool142_Plate4-1-A22-F2


title: Pool142_Plate4-1-A22-F13


title: Pool142_Plate4-1-A22-E14


title: Pool142_Plate4-1-A22-E13


title: Pool142_Plate4-1-A22-K13


title: Pool142_Plate4-1-A22-K2


title: Pool142_Plate4-1-A22-C14


title: Pool142_Plate4-1-A22-C13


title: Pool142_Plate4-1-A22-D1


title: Pool142_Plate4-1-A22-E1


title: Pool142_Plate4-1-A22-D14


title: Pool142_Plate4-1-A22-L13


title: Pool142_Plate4-1-A22-C1


title: Pool142_Plate4-1-A22-B14


title: Pool142_Plate4-1-A22-A14


title: Pool142_Plate4-1-A22-B13


title: Pool142_Plate4-1-A22-A13


title: Pool142_Plate3-6-K18-O23

value: DGa


title: Pool142_Plate3-6-K18-P11

value: DGp


title: Pool142_Plate3-6-K18-N23

value: male


title: Pool142_Plate3-6-K18-P12

value: female


title: Pool142_Plate3-6-K18-M12


title: Pool142_Plate3-6-K18-M23


title: Pool142_Plate3-6-K18-M11


title: Pool142_Plate3-6-K18-N12


title: Pool142_Plate3-6-K18-L23


title: Pool142_Plate3-6-K18-L12


title: Pool142_Plate3-6-K18-K23


title: Pool142_Plate3-6-K18-K12


title: Pool142_Plate3-6-K18-K24


title: Pool142_Plate3-6-K18-K11


title: Pool142_Plate3-6-K18-L11


title: Pool142_Plate3-6-K18-J23


title: Pool142_Plate3-6-K18-I24


title: Pool142_Plate3-6-K18-I11


title: Pool142_Plate3-6-K18-H24


title: Pool142_Plate3-6-K18-I23


title: Pool142_Plate3-6-K18-J11


title: Pool142_Plate3-6-K18-J24


title: Pool142_Plate3-6-K18-O12


title: Pool142_Plate3-6-K18-B24


title: Pool142_Plate3-6-K18-C11


title: Pool142_Plate3-6-K18-E11


title: Pool142_Plate3-6-K18-C23


title: Pool142_Plate3-6-K18-C12


title: Pool142_Plate3-6-K18-F11


title: Pool142_Plate3-6-K18-F12


title: Pool142_Plate3-6-K18-H23


title: Pool142_Plate3-6-K18-G11


title: Pool142_Plate3-6-K18-D24


title: Pool142_Plate3-6-K18-D23


title: Pool142_Plate3-6-K18-E12


title: Pool142_Plate3-6-K18-H12


title: Pool142_Plate3-6-K18-G24


title: Pool142_Plate3-6-K18-H11


title: Pool142_Plate3-6-K18-B23


title: Pool142_Plate3-6-K18-F24


title: Pool142_Plate3-6-K18-G12


title: Pool142_Plate3-5-K18-O21


title: Pool142_Plate3-5-K18-O10


title: Pool142_Plate3-6-K18-A23


title: Pool142_Plate3-5-K18-P22


title: Pool142_Plate3-5-K18-P10


title: Pool142_Plate3-6-K18-A12


title: Pool142_Plate3-6-K18-B11


title: Pool142_Plate3-5-K18-O22


title: Pool142_Plate3-6-K18-A11


title: Pool142_Plate3-5-K18-O9


title: Pool142_Plate3-5-K18-P9


title: Pool142_Plate3-5-K18-N22


title: Pool142_Plate3-5-K18-N10


title: Pool142_Plate3-5-K18-I9


title: Pool142_Plate3-5-K18-L10


title: Pool142_Plate3-5-K18-M10


title: Pool142_Plate3-5-K18-M21


title: Pool142_Plate3-5-K18-K9


title: Pool142_Plate3-5-K18-J10


title: Pool142_Plate3-5-K18-M9


title: Pool142_Plate3-5-K18-K22


title: Pool142_Plate3-5-K18-L22


title: Pool142_Plate3-5-K18-H22


title: Pool142_Plate3-5-K18-H21


title: Pool142_Plate3-5-K18-I10


title: Pool142_Plate3-5-K18-I22


title: Pool142_Plate3-5-K18-H10


title: Pool142_Plate3-5-K18-D10


title: Pool142_Plate3-5-K18-C22


title: Pool142_Plate3-5-K18-L21


title: Pool142_Plate3-5-K18-D22


title: Pool142_Plate3-5-K18-G22


title: Pool142_Plate3-5-K18-D9


title: Pool142_Plate3-5-K18-E22


title: Pool142_Plate3-5-K18-E21


title: Pool142_Plate3-5-K18-G21


title: Pool142_Plate3-5-K18-F21


title: Pool142_Plate3-5-K18-E9


title: Pool142_Plate3-5-K18-J21


title: Pool142_Plate3-5-K18-C21


title: Pool142_Plate3-5-K18-B9


title: Pool142_Plate3-5-K18-B22


title: Pool142_Plate3-5-K18-D21


title: Pool142_Plate3-5-K18-F22


title: Pool142_Plate3-5-K18-F10


title: Pool142_Plate3-5-K18-A9


title: Pool142_Plate3-5-K18-A21


title: Pool142_Plate3-4-K18-P19


title: Pool142_Plate3-4-K18-P7


title: Pool142_Plate3-5-K18-A10


title: Pool142_Plate3-4-K18-O8


title: Pool142_Plate3-5-K18-B10


title: Pool142_Plate3-4-K18-N19


title: Pool142_Plate3-4-K18-N20


title: Pool142_Plate3-4-K18-P8


title: Pool142_Plate3-4-K18-N8


title: Pool142_Plate3-4-K18-O20


title: Pool142_Plate3-4-K18-M7


title: Pool142_Plate3-4-K18-M8


title: Pool142_Plate3-4-K18-M20


title: Pool142_Plate3-4-K18-L7


title: Pool142_Plate3-4-K18-K19


title: Pool142_Plate3-4-K18-J8


title: Pool142_Plate3-4-K18-L8


title: Pool142_Plate3-4-K18-L20


title: Pool142_Plate3-4-K18-M19


title: Pool142_Plate3-4-K18-J7


title: Pool142_Plate3-4-K18-K7


title: Pool142_Plate3-4-K18-J20


title: Pool142_Plate3-4-K18-I8


title: Pool142_Plate3-4-K18-I7


title: Pool142_Plate3-4-K18-I19


title: Pool142_Plate3-4-K18-H7


title: Pool142_Plate3-4-K18-H8


title: Pool142_Plate3-4-K18-H19


title: Pool142_Plate3-4-K18-G20


title: Pool142_Plate3-4-K18-G8


title: Pool142_Plate3-4-K18-G19


title: Pool142_Plate3-4-K18-F19


title: Pool142_Plate3-4-K18-G7


title: Pool142_Plate3-4-K18-L19


title: Pool142_Plate3-4-K18-F8


title: Pool142_Plate3-4-K18-F20


title: Pool142_Plate3-4-K18-D7


title: Pool142_Plate3-4-K18-E19


title: Pool142_Plate3-4-K18-E7


title: Pool142_Plate3-4-K18-D19


title: Pool142_Plate3-4-K18-C19


title: Pool142_Plate3-4-K18-C8


title: Pool142_Plate3-4-K18-B8


title: Pool142_Plate3-4-K18-B19


title: Pool142_Plate3-4-K18-A19


title: Pool142_Plate3-4-K18-A8


title: Pool142_Plate3-4-K18-A7


title: Pool142_Plate3-3-K18-P6


title: Pool142_Plate3-3-K18-P17


title: Pool142_Plate3-4-K18-H20


title: Pool142_Plate3-3-K18-P18


title: Pool142_Plate3-3-K18-O17


title: Pool142_Plate3-3-K18-N18


title: Pool142_Plate3-3-K18-M5


title: Pool142_Plate3-4-K18-B20


title: Pool142_Plate3-3-K18-M17


title: Pool142_Plate3-3-K18-L5


title: Pool142_Plate3-3-K18-L6


title: Pool142_Plate3-3-K18-K6


title: Pool142_Plate3-3-K18-O6


title: Pool142_Plate3-3-K18-K17


title: Pool142_Plate3-3-K18-I5


title: Pool142_Plate3-3-K18-J17


title: Pool142_Plate3-3-K18-I18


title: Pool142_Plate3-3-K18-J6


title: Pool142_Plate3-3-K18-J5


title: Pool142_Plate3-3-K18-K18


title: Pool142_Plate3-3-K18-J18


title: Pool142_Plate3-3-K18-G17


title: Pool142_Plate3-3-K18-G18


title: Pool142_Plate3-3-K18-G5


title: Pool142_Plate3-3-K18-G6


title: Pool142_Plate3-3-K18-I17


title: Pool142_Plate3-3-K18-H18


title: Pool142_Plate3-3-K18-H17


title: Pool142_Plate3-3-K18-F6


title: Pool142_Plate3-3-K18-F18


title: Pool142_Plate3-3-K18-F17


title: Pool142_Plate3-3-K18-F5


title: Pool142_Plate3-3-K18-E18


title: Pool142_Plate3-3-K18-L18


title: Pool142_Plate3-3-K18-L17


title: Pool142_Plate3-3-K18-E17


title: Pool142_Plate3-3-K18-D5


title: Pool142_Plate3-3-K18-C5


title: Pool142_Plate3-3-K18-D18


title: Pool142_Plate3-3-K18-C17


title: Pool142_Plate3-3-K18-B17


title: Pool142_Plate3-3-K18-B18


title: Pool142_Plate3-2-K18-O15


title: Pool142_Plate3-2-K18-N15


title: Pool142_Plate3-2-K18-P16


title: Pool142_Plate3-2-K18-K3


title: Pool142_Plate3-2-K18-L15


title: Pool142_Plate3-2-K18-M3


title: Pool142_Plate3-2-K18-K16


title: Pool142_Plate3-2-K18-P15


title: Pool142_Plate3-2-K18-P4


title: Pool142_Plate3-2-K18-J3


title: Pool142_Plate3-2-K18-M16


title: Pool142_Plate3-2-K18-K15


title: Pool142_Plate3-2-K18-L16


title: Pool142_Plate3-2-K18-I15


title: Pool142_Plate3-2-K18-J15


title: Pool142_Plate3-2-K18-I16


title: Pool142_Plate3-2-K18-L3


title: Pool142_Plate3-2-K18-M15


title: Pool142_Plate3-2-K18-J4


title: Pool142_Plate3-2-K18-H3


title: Pool142_Plate3-2-K18-H15


title: Pool142_Plate3-2-K18-J16


title: Pool142_Plate3-2-K18-H16


title: Pool142_Plate3-2-K18-G3


title: Pool142_Plate3-2-K18-G15


title: Pool142_Plate3-2-K18-G4


title: Pool142_Plate3-2-K18-G16


title: Pool142_Plate3-2-K18-F4


title: Pool142_Plate3-2-K18-F16


title: Pool142_Plate3-2-K18-F3


title: Pool142_Plate3-2-K18-B4


title: Pool142_Plate3-2-K18-E4


title: Pool142_Plate3-2-K18-E16


title: Pool142_Plate3-2-K18-D3


title: Pool142_Plate3-2-K18-B3


title: Pool142_Plate3-2-K18-C16


title: Pool142_Plate3-2-K18-D15


title: Pool142_Plate3-1-K18-P2


title: Pool142_Plate3-2-K18-E15


title: Pool142_Plate3-2-K18-B16


title: Pool142_Plate3-2-K18-A15


title: Pool142_Plate3-2-K18-B15


title: Pool142_Plate3-2-K18-C3


title: Pool142_Plate3-2-K18-E3


title: Pool142_Plate3-2-K18-C4


title: Pool142_Plate3-2-K18-A3


title: Pool142_Plate3-1-K18-O13


title: Pool142_Plate3-1-K18-O1


title: Pool142_Plate3-1-K18-N2


title: Pool142_Plate3-1-K18-P13


title: Pool142_Plate3-1-K18-O14


title: Pool142_Plate3-1-K18-M2


title: Pool142_Plate3-1-K18-N13


title: Pool142_Plate3-1-K18-M1


title: Pool142_Plate3-1-K18-M13


title: Pool142_Plate3-2-K18-D16


title: Pool142_Plate3-1-K18-L1


title: Pool142_Plate3-1-K18-L13


title: Pool142_Plate3-1-K18-P14


title: Pool142_Plate3-1-K18-L14


title: Pool142_Plate3-1-K18-J14


title: Pool142_Plate3-1-K18-K1


title: Pool142_Plate3-1-K18-K13


title: Pool142_Plate3-1-K18-J13


title: Pool142_Plate3-1-K18-I2


title: Pool142_Plate3-1-K18-E1


title: Pool142_Plate3-1-K18-B1


title: Pool142_Plate3-1-K18-D14


title: Pool142_Plate3-1-K18-G13


title: Pool142_Plate3-1-K18-C13


title: Pool142_Plate3-1-K18-A14


title: Pool142_Plate3-1-K18-I13


title: Pool142_Plate3-1-K18-E13


title: Pool142_Plate3-1-K18-B14


title: Pool142_Plate3-1-K18-F14


title: Pool142_Plate3-1-K18-F1


title: Pool142_Plate3-1-K18-B2


title: Pool142_Plate3-1-K18-C2


title: Pool142_Plate2-6-O21-P24

value: CAp


title: Pool142_Plate3-1-K18-D13


title: Pool142_Plate3-1-K18-A1


title: Pool142_Plate2-6-O21-P23


title: Pool142_Plate3-1-K18-I14


title: Pool142_Plate3-1-K18-C1


title: Pool142_Plate3-1-K18-G1


title: Pool142_Plate3-1-K18-H14


title: Pool142_Plate2-6-O21-P12


title: Pool142_Plate2-6-O21-N24


title: Pool142_Plate2-6-O21-O12

value: CAa


title: Pool142_Plate2-6-O21-O24


title: Pool142_Plate2-6-O21-O23


title: Pool142_Plate2-6-O21-K11


title: Pool142_Plate2-6-O21-M23


title: Pool142_Plate2-6-O21-J12


title: Pool142_Plate2-6-O21-L24


title: Pool142_Plate2-6-O21-J23


title: Pool142_Plate2-6-O21-M11


title: Pool142_Plate2-6-O21-L23


title: Pool142_Plate2-6-O21-L11


title: Pool142_Plate2-6-O21-J11


title: Pool142_Plate2-6-O21-H24


title: Pool142_Plate2-6-O21-N11


title: Pool142_Plate2-6-O21-N12


title: Pool142_Plate2-6-O21-I23


title: Pool142_Plate2-6-O21-I11


title: Pool142_Plate3-1-K18-A13


title: Pool142_Plate2-6-O21-H12


title: Pool142_Plate2-6-O21-H11


title: Pool142_Plate2-6-O21-H23


title: Pool142_Plate2-6-O21-G12


title: Pool142_Plate2-6-O21-F24


title: Pool142_Plate2-6-O21-F23


title: Pool142_Plate2-6-O21-K24


title: Pool142_Plate2-6-O21-K12


title: Pool142_Plate2-6-O21-F11


title: Pool142_Plate2-6-O21-E24


title: Pool142_Plate2-6-O21-D11


title: Pool142_Plate2-6-O21-D23


title: Pool142_Plate2-6-O21-D24


title: Pool142_Plate2-5-O21-P9


title: Pool142_Plate2-6-O21-C24


title: Pool142_Plate2-6-O21-A23


title: Pool142_Plate2-6-O21-A11


title: Pool142_Plate2-6-O21-E11


title: Pool142_Plate2-6-O21-E12


title: Pool142_Plate2-6-O21-C12


title: Pool142_Plate2-6-O21-B23


title: Pool142_Plate2-6-O21-B11


title: Pool142_Plate2-5-O21-O21


title: Pool142_Plate2-5-O21-N9


title: Pool142_Plate2-5-O21-O9


title: Pool142_Plate2-5-O21-P10


title: Pool142_Plate2-5-O21-N22


title: Pool142_Plate2-5-O21-N21


title: Pool142_Plate2-5-O21-N10


title: Pool142_Plate2-5-O21-M10


title: Pool142_Plate2-5-O21-M22


title: Pool142_Plate2-6-O21-C23


title: Pool142_Plate2-6-O21-D12


title: Pool142_Plate2-5-O21-L21


title: Pool142_Plate2-5-O21-L22


title: Pool142_Plate2-5-O21-P22


title: Pool142_Plate2-5-O21-K22


title: Pool142_Plate3-1-K18-H2


title: Pool142_Plate2-5-O21-K21


title: Pool142_Plate2-5-O21-G21


title: Pool142_Plate2-5-O21-G9


title: Pool142_Plate2-5-O21-H10


title: Pool142_Plate2-5-O21-G10


title: Pool142_Plate2-5-O21-J10


title: Pool142_Plate2-5-O21-I9


title: Pool142_Plate2-5-O21-H21


title: Pool142_Plate2-5-O21-J22


title: Pool142_Plate2-5-O21-I21


title: Pool142_Plate2-5-O21-J21


title: Pool142_Plate2-5-O21-G22


title: Pool142_Plate2-5-O21-I10


title: Pool142_Plate2-5-O21-K10


title: Pool142_Plate2-5-O21-I22


title: Pool142_Plate2-5-O21-H22


title: Pool142_Plate2-5-O21-E9


title: Pool142_Plate2-5-O21-F21


title: Pool142_Plate2-5-O21-E22


title: Pool142_Plate2-5-O21-E10


title: Pool142_Plate2-5-O21-E21


title: Pool142_Plate2-5-O21-L10


title: Pool142_Plate2-5-O21-D21


title: Pool142_Plate2-5-O21-D10


title: Pool142_Plate2-5-O21-C9


title: Pool142_Plate2-5-O21-C22


title: Pool142_Plate2-5-O21-B9


title: Pool142_Plate2-5-O21-A10


title: Pool142_Plate2-5-O21-A22


title: Pool142_Plate2-5-O21-B21


title: Pool142_Plate2-4-O21-P20


title: Pool142_Plate2-4-O21-N20


title: Pool142_Plate2-4-O21-O20


title: Pool142_Plate2-4-O21-P19


title: Pool142_Plate2-5-O21-F9


title: Pool142_Plate2-4-O21-N8


title: Pool142_Plate2-4-O21-N19


title: Pool142_Plate2-4-O21-P8


title: Pool142_Plate2-4-O21-O8


title: Pool142_Plate2-5-O21-D9


title: Pool142_Plate2-4-O21-N7


title: Pool142_Plate2-5-O21-A21


title: Pool142_Plate2-4-O21-M19


title: Pool142_Plate2-4-O21-L8


title: Pool142_Plate2-4-O21-L20


title: Pool142_Plate2-4-O21-K7


title: Pool142_Plate2-4-O21-L19


title: Pool142_Plate2-4-O21-L7


title: Pool142_Plate2-4-O21-J8


title: Pool142_Plate2-4-O21-M8


title: Pool142_Plate2-4-O21-J7


title: Pool142_Plate2-4-O21-J20


title: Pool142_Plate2-4-O21-I8


title: Pool142_Plate2-4-O21-H8


title: Pool142_Plate2-4-O21-F8


title: Pool142_Plate2-4-O21-H19


title: Pool142_Plate2-4-O21-H7


title: Pool142_Plate2-4-O21-I19


title: Pool142_Plate2-4-O21-G19


title: Pool142_Plate2-4-O21-F20


title: Pool142_Plate2-4-O21-F7


title: Pool142_Plate2-4-O21-G20


title: Pool142_Plate2-4-O21-E20


title: Pool142_Plate2-4-O21-E19


title: Pool142_Plate2-4-O21-D8


title: Pool142_Plate2-4-O21-E7


title: Pool142_Plate2-4-O21-B8


title: Pool142_Plate2-4-O21-B20


title: Pool142_Plate2-4-O21-B7


title: Pool142_Plate2-4-O21-A8


title: Pool142_Plate2-4-O21-D7


title: Pool142_Plate2-4-O21-J19


title: Pool142_Plate2-4-O21-A20


title: Pool142_Plate2-4-O21-G8


title: Pool142_Plate2-3-O21-P18


title: Pool142_Plate2-3-O21-P17


title: Pool142_Plate2-3-O21-O5


title: Pool142_Plate2-4-O21-F19


title: Pool142_Plate2-3-O21-O18


title: Pool142_Plate2-4-O21-E8


title: Pool142_Plate2-3-O21-O17


title: Pool142_Plate2-3-O21-N6


title: Pool142_Plate2-3-O21-N18


title: Pool142_Plate2-3-O21-N17


title: Pool142_Plate2-3-O21-M18


title: Pool142_Plate2-3-O21-L18


title: Pool142_Plate3-1-K18-H13


title: Pool142_Plate2-3-O21-L6


title: Pool142_Plate2-3-O21-L5


title: Pool142_Plate2-3-O21-M17


title: Pool142_Plate2-3-O21-K5


title: Pool142_Plate2-3-O21-L17


title: Pool142_Plate2-3-O21-K6


title: Pool142_Plate2-3-O21-K18


title: Pool142_Plate2-3-O21-I6