title: Extracelluar vesicles from Knockout IMP2 HCT116 cells miRNA-seq - replicate 1 [EV_KO1]

city: Rochester

first: Ernesto

last: Aparicio-Puerta

address: 265 Crittenden Boulevard, CU 420630

department: Biostatistics and Computational Biology

institute: University of Rochester

description: MGIEasy miRNA-seq

data_processing:
Data was preprocessed, aligned and quantified using miRMaster
Assembly: miRBase release 22.1
Supplementary files format and content: A tsv (tab-delimited) file containing the miRNA raw counts using miRBase release 22.1 as reference.

growth_protocol: HCT116 WT and IMP2 KO cells were cultured in DMEM medium with 10% FBS. After they reached 70% confluency, the medium was replaced with EV-depleted media. Tumor-conditioned media was collected 48 h after for EV isolation.

extract_protocol:
RNAs from EVs and cells were isolated using miRNeasy Serum/Plasma kit (#217184, Qiagen) and High Pure RNA Isolation Kit (#11828665001, Roche Diagnostics), respectively, according to the manufacturer’s protocols. RNA concentration was quantified by Nanodrop spectrometer (ThermoFisher Scientific, USA) at 260 nm.
small RNA library was prepared according to the MGIEasy small RNA library preparation kit (#1000005269, China). Briefly, after 3’ and 5’ adapter ligation, cDNA was synthesized. After PCR amplification, the product was loaded on a 6% TBE PAGE gel and the portion of the gel corresponding to the appropriate size (100-120 bp) was extracted. The purified PCR product was subjected to denaturation and single-strand circularization, followed by enzymatic digestion and cleanup. The purified product was quantified with a Qubit ssDNA assay kit (#Q10212, Invitrogen).

value: colorectal cancer

value: KO IMP2


title: Extracelluar vesicles from Knockout IMP2 HCT116 cells miRNA-seq - replicate 3 [EV_KO3]

status: Public on Jun 21 2023

type: SRA

state: NY

country: USA

description: MGIEasy miRNA-seq

library_source: transcriptomic

molecule: total RNA

organism: Homo sapiens

tag: cell line

tag: cell type

tag: genotype


title: Extracelluar vesicles from Knockout IMP2 HCT116 cells miRNA-seq - replicate 2 [EV_KO2]

description: MGIEasy miRNA-seq


title: H1-2

type: genomic

city: Duarte

first: wei

last: chen

state: CA

address: 1500 E Duarte Rd

department: Cancer Biology-BRI

institute: City of Hope National Medical Center

description:
24086_H1D0_2
Processed data file: 201496710238_201496710121.igv
850 beadchip

hyb_protocol: The bisulfite converted DNAs were denatured by the 0.1N NaOH and amplified for 20 – 24 hours in the 37C hybridization chamber to produce a sufficient amount of  the DNA sample. The amplified DNAs were enzymatically fragmented on the 37C heat block for 1 hour and were precipitated for 30 min at 4C. For the hybridizing the DNAs onto Illumina beadchips, the precipitated DNAs were resuspended by RA1 solution according to the manufacturer protocol and the suspended DNAs with an appropriate volume were loaded onto the Inf Methyl EPIC 8 sample beadchip (San Diego, CA). The hybridication was performed for over the 16 hours within 24 hours.

scan_protocol: The image acquisition was carried out using the Illumina iScan System (San Diego, CA).

data_processing:
Raw data were processed and normalized by "SWAN" method using Bioconductor package "lumi".
The *igv processed data files represent SWAN-normalized Average Beta values.

label: Cy5 and Cy3

molecule: genomic DNA

source_name: H1 hESC control biological replicate 2

label_protocol: We followed the standard Infinium HD Methylation protocol guide and all reagents, which were required in the procedure, were purchased from the Illumina (San Diego, CA).

extract_protocol:
genomic DNA was extracted and purified from cells using Qiagen DNeasy Kit according to standard instructions
[bisulfite conversion protocol] The non-methylated cytosine residues of the purified genomic DNA sample were converted into uracil using EZ DNA Methylation Kit from Zymo Research (Irvine, CA).

value: pluripotent stem cell line

tag: integration of cpg free insert

value: N

tag: dna methylation at target cgi


title: Tag1A2_Tag01

city: Munich

first: Peter

last: Androvic

state: Bavaria

address: Feodor-Lynen-Strasse 17

country: Germany

institute: LMU Hospital, Institute for Stroke and Dementia Research

library_source: transcriptomic single cell

data_processing:
Fastq files were quality-controlled with FastQC and reads were then aligned using rnaSTAR to the GRCm38 (mm10) genome with addition of ERCC spike-in sequences. To obtain single cell gene expression matrices reads were counted with rnaSTAR using parameter “quantMode GeneCounts” and unstranded argument.
Assembly: mm10
Supplementary files format and content: Tab-separated counts per cell and cell metadata

organism: Mus musculus

extract_protocol:
18 days after LPC injection, mice were deeply anesthetized and perfused with cold HBSS between 9am-11am (to decrease circadian fluctuations). Each brain was removed and under a dissection microscope individually micro-dissected; gray matter was isolated from the frontal cortex and white matter was isolated form optic tract, medial lemniscus and corpus callosum (attached gray matter and choroid plexus were carefully removed). Single cells were isolated using GentleMacs with the Neural Tissue Dissociation Kit (Papain) (Miltenyi Biotec). We added actinomycin D to a final concentration of 45 mM into the dissociation solution and enzyme mix to prevent ex-vivo transcription. The dissociated cell suspension was passed through a 70 mm cell strainer before labeling. Subsequently, cells were blocked with mouse FcR-blocking reagent (CD16/CD32 Monoclonal Antibody) and then stained for with the antibodiy against CD11b (PE/Cy7,M1/70, eBioscience, Cat:48-0451-82,1:200) and afterwards washed with PBS (Sigma, D8537). Cells were then stained with DAPI (4’,6-diamidino-2-phenylindole, 1:10000 dilution; Sigma)  and viable (DAPI-negative) single CD11b-positive cells were sorted by flow cytometry (SH800; Sony) into 96 well plates filled with 4 mL lysis buffer containing 0.05% Triton X-100 (Sigma) and, ERCC spike-in Mix (1:24000000 dilution), 2.5 mM oligo-dT, 2.5 mM dNTP and 2 U/mL of recombinant RNase inhibitor (Clontech).
The 96-well plates containing the sorted single cells were thawed and incubated for 3 min at 72°C and thereafter immediately placed on ice. To perform reverse transcription (RT) we added into each well a master mix of 0.59 μL H2O, 0.5 μL SMARTScribe™ Reverse Transcriptase (Clontech), 2 μL 5x First Strand buffer, 0.25 μL Recombinant RNase Inhibitor (Clontech), 2 μL Betaine (5 M Sigma), 0.5 μL DTT (100 mM) 0.06 μL MgCl2 (1 M Sigma), 0.1 μL Templateswitching oligos (TSO) (100 μM AAGCAGTGGTATCAACGCAGAGTACrGrG+G). Next RT reactions were incubated at 42°C for 90 min followed by 70°C for 5 min and 10 cycles of 50°C 2 min, 42°C 2 min; ending with 70°C for 5 min for enzyme inactivation. Preamplification of cDNA was performed by adding 12.5 μL KAPA HiFi Hotstart 2x (KAPA Biosystems), 2.138 μL H2O, 0.25 μL ISPCR primers (10 μM, 5’ AAGCAGTGGTATCAACGCAGAGT-3), 0.1125 μL Lambda Exonuclease under the following conditions: 37°C for 30 min, 95°C for 3 min, 23 cycles of (98°C for 20 sec, 67°C for 15 sec, 72°C for 4 min), and a final extension at 72°C for 5 min. Libraries were then cleaned using AMPure beads (Beckman-Coulter) cleanup at a 0.7:1 ratio of beads to PCR product. Libraries were assessed by Bio-analyzer (Agilent 2100), using the High Sensitivity DNA analysis kit, and quantified using Qubit’s DNA HS assay kits and a Qubit 4.0 Fluorometer (Invitrogen, LifeTechnologies). Further selection of samples was performed via qPCR assay against ubiquitin transcripts Ubb77 (primer 1 5’-GGAGAGTCCATCGTGGTTATTT-3’ primer 2 5’-ACCTCTAGGGTGATGGTCTT-3’, probe 5’-/5Cy5/TGCAGATCTTCGTGAAGACCTGAC/3IAbRQSp/-3’) measured on a LightCycler 480 Instrument II (Roche). Samples were normalized to 160 pg/μL. Sequencing libraries were constructed by using in-house produced Tn5 transposase. Libraries were barcoded, pooled and purified in 3 rounds of AMPure bead (Beckman-Coulter) cleanup at a 0.8:1 ratio of beads to library. Libraries were then sequenced with 100 bp paired-end sequencing on DNBSeq platform (BGI group).

treatment_protocol: Lysophosphatidylcholine (LPC) injections were administered at the age of 16-18 weeks. A solution of 1% LPC (L4129, Sigma) in PBS was mixed with Monastral blue (274011, SigmaAldrich) at a concentration of 0.03% to aid with visualization of the lesion during tissue processing. Mice were anaesthetized with an intraperitoneal injection of MMF solution (0.5 mg medetomidin/kg (body weight), 5.0 mg midazolam/kg (body weight) and 0.05 mg fentanyl/kg (body weight)). Then, head fur was removed, the eyes were treated with bepanthene cream (1578847, Bayer) and a small incision in the skin was performed to expose the skull. The mouse was positioned into a stereotactic injection apparatus and a small hole was drilled at the following injection coordinates (from bregma): X, ± 1.0 mm; Y, −0.1 mm). A glass capillary containing the LPC–monastral blue solution was then lowered to Z: −1.30 mm from bregma, and 1 µL was injected at a rate of 100 nL/minute. Two minutes after the delivery of LPC, the capillary was slowly retracted. The mouse was then injected with 0.05 mg buprenorphin/kg (body weight), and the skin was sutured. Anesthesia was terminated by a subcutaneous injection of AFN solution, containing 2.5 mg/kg (body weight) atipamezol, 1.2 mg/kg (body weight) naloxon and 0.5 mg/kg (body weight) flumazenil.

value: CD11b+ cells

tag: tissue

tag: strain

tag: age


title: Extracelluar vesicles from Knockout IMP2 HCT116 cells miRNA-seq - replicate 4 [EV_KO4]

description: MGIEasy miRNA-seq


title: ONT_T1_BR2

city: Cambridge

first: Louise

last: Matheson

address: Babraham Research Campus

department: Laboratory of Lymphocyte Signalling and Development

country: United Kingdom

institute: The Babraham Institute

data_processing:
Basecalling of reads was performed with guppy basecaller (4.0.11) in high accuracy mode. Reads with a mean sequence quality score higher than 7 were demultiplexed with the guppy_barcoder tool and processed with pychopper (2.5.0) in order to identify, orient and trim the full-length cDNA reads.
Pychopped reads were then aligned to mouse genome reference GRCm38 using minimap2 (2.17-r941) (Li et al, 2018, Bioinformatics, DOI: 10.1093/bioinformatics/bty191) with splice aware setting and analysed with FLAIR pipeline (Tang et al, 2020, Nat Commun, DOI: 10.1038/s41467-020-15171-6) for isoform identification.
Assembly: GRCm38
Supplementary files format and content: ONT_cDNAPCR_fullLength_counts.tsv: tab-delimited text file containing gene names and IDs together with the number of full length transcript isoforms detected for each gene in each sample, from FLAIR analysis.

source_name: spleen

growth_protocol: Single cell suspensions were prepared from spleen by passing the organ through a 70mm cell strainer. B cells were pre-enriched through negative selection (Miltenyi B cell isolation kit, cat # 130-090-862) and T1, T2, MZ and FoB cell subsets FACS-sorted using anti-CD19 (6D5), anti-CD93 (AA4.1), anti-CD23 (B3B4), anti-CD21 (7G6) and anti-IgM (II/41) antibodies.

extract_protocol:
Oxford Nanopore Technologies (ONT) sequencing libraries were prepared as described previously (Berrens et al, 2021, Nat. Biotech, DOI: 10.1038/s41587-021-01093-1; D'Angeli et al, 2022, Eur J Immunol, DOI: 10.1002/eji.202149781). Briefly, RNA was reverse transcribed using the Smart-seq2 protocol (Picelli et al, 2013, Nat Methods, DOI: 10.1038/nmeth.2639), cDNA was amplified using the KAPA HiFi Uracil+ hot start polymerase mix (Roche) and PCR products were purified using 0.6X AMPure XP beads (Beckman). Equal amount of cDNA libraries were pooled for a total of 200 fmol and sequenced with MinION R9.4.1 flow cell using the SQK-LSK109 kit on MinKNOW (21.02.1) according to manufacturers’ instructions.
Total RNA was extracted using RNeasy Mini Kit (Qiagen)

treatment_protocol: B cell subsets were purified from 12-week old untreated mice

value: spleen

value: transitional 1 B cells

tag: Sex

value: male

tag: flow cytometry_markers


title: ONT_T1_BR1


title: Extracelluar vesicles from Wild type HCT116 cells miRNA-seq - replicate 1 [EV_WT1]

description: MGIEasy miRNA-seq

value: WT


title: OS-22

city: Campinas

first: Mariana

last: Maschietto

state: São Paulo

address: Rua Márcia Mendes, 619

department: Research Center

country: Brazil

institute: Boldrini Children's Hospital

hyb_protocol: DNA was bisulfite converted using the EZ DNA Methylation kit (Zymo Research Corp), according to manufacturer recommendations, and hybridized in the Human Methylation 450 BeadChip microarrays (HM450K, Illumina), following the Illumina Infinium HD methylation protocol.

scan_protocol: Raw data was extracted using the iScan SQ scanner (Illumina) by GenomeStudio software (v.2011.1), with the methylation module v.1.9.0 (Illumina), into IDAT files,

data_processing: The R package ChAMP was applied to the dataset. Signal intensities from probes type I and II were normalized using the updated version of BMIQ method.

source_name: osteosarcoma

label_protocol: Standard Illumina protocol

extract_protocol: DNA was isolated using the QIAsymphony equipment with the DSP DNA Mini Kit (Qiagen)

tag: subtypes

value: osteoblastic

value: female

tag: age (years)

tag: metastasis

tag: huvos grade

tag: tumor size (cm)


title: OS-21


title: OS-19

value: fibroblastic


title: H1 Control (46p)

description:
24084_MLH1_H1P37_9
Processed data file: 201496710238_201496710121.igv
850 beadchip

source_name: H1 long-term passaging, 46 passages total


title: H1-1

description:
24085_H1D0_1
Processed data file: 201496710238_201496710121.igv
850 beadchip

source_name: H1 hESC control


title: Extracelluar vesicles from Wild type HCT116 cells miRNA-seq - replicate 2 [EV_WT2]

description: MGIEasy miRNA-seq


title: Extracelluar vesicles from Wild type HCT116 cells miRNA-seq - replicate 3 [EV_WT3]

description: MGIEasy miRNA-seq


title: CpGF-PI-ssI pool

description:
21331_MLH1-M-pre-CRE
Processed data file: 202047860114.igv
850 beadchip

source_name: H1 hESC neomycin resistant pool with ssI at MLH1 promoter CGI

value: Y


title: Extracelluar vesicles from Wild type HCT116 cells miRNA-seq - replicate 4 [EV_WT4]

description: MGIEasy miRNA-seq


title: Knockout IMP2 HCT116 cells miRNA-seq - replicate 1 [VLC_KO1]

description: MGIEasy miRNA-seq


title: OS-18


title: ssI-C4

description:
21611_M-17
Processed data file: 202139520099.igv
850 beadchip

source_name: H1 hESC clone with ssI at MLH1 promoter CGI


title: CpGF-PI-NeoR pool

description:
21334_MLH1-P-pre-CRE
Processed data file: 202047860114.igv
850 beadchip

source_name: H1 hESC neomycin resistant pool with plasmid insert at MLH1 promoter CGI


title: Knockout IMP2 HCT116 cells miRNA-seq - replicate 2 [VLC_KO2]

description: MGIEasy miRNA-seq


title: Liver-T48-Rep4

type: RNA

city: TOULOUSE cedex 3

first: Léon

last: Kautz

address: CHU Purpan - Place du Dr Baylac - CS 60 039

department: IRSD U1220

country: France

institute: INSERM

hyb_protocol: 600 ng of Cy3-labelled cRNA (specific activity >6 pmol Cy3/µg cRNA) was fragmented at 60°C for 30 minutes in a reaction volume of 25 µl containing 10x Agilent fragmentation buffer and 25x Agilent blocking agent following the manufacturer's instructions (Agilent Technologies, Santa Clara, CA). On completion of the fragmentation reaction, 25 µl of 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to SurePrint G3 Mouse GE v2 microarray (8X60K, Design 074809  enclosed in Agilent SureHyb-enabled hybridization chambers for 17 hours at 65°C in a rotating Agilent hybridization oven. After hybridization, microarrays were washed sequentially in Wash buffer 1 (Agilent Technologies, 1 min) and Wash buffer 2 (Agilent Technologies, 37°C, 1 min).

scan_protocol: Slides were scanned immediately after washing on a Agilent G2505C Microarray Scanner with Agilent Scan Control A.8.5.1 software

data_processing: The scanned images were analyzed with Feature Extraction Software 10.10.1.1 (Agilent Technologies, Santa Clara, CA) using default parameters (protocol GE1_1010_Sep10 and Grid: 074809_D_F_20150624). All subsequent data analyses were done under R (www.r-project.org) using packages of Bioconductor (www.bioconductor.org). Raw data (median of pixels intensity) were imported into R using the read.maimages function from the limma package with the following weight function (assigning a weight of 1 or 0 to each spot): myfunw<-function(x) {okType<-x$ControlType==0; okFoundGreen<-x$gIsFound==1; okPos=x$gIsPosAndSignif==1; okWellAbove<- x$gIsWellAboveBG==1; as.numeric(okType & okFoundGreen & okPos & okWellAbove);} We selected the spots with a minimal weight of 1 for 10 out of 12 microarrays or with a minimal weight of 2 per group from at least one experimental group. At this step, 35830 spots out of 62976 were selected. Data were then stored in an ExpressionSet object and normalized by the quantile method using the normalize.quantiles function from the preprocessCore R library. Replicated probes on the array (identical ProbeName) were resolved by taking the median normalized signal of each set of replicated probes. The resulting matrix has 33178 rows each corresponding to a unique ProbeName (provided as data Matrix).

source_name: Liver

label_protocol: For each sample, Cyanine-3 (Cy3) labeled cRNA was prepared from 200 ng of total RNA using the One-Color Quick Amp Labeling kit (Agilent Technologies, Santa Clara, CA) according to the manufacturer's instructions, followed by Agencourt RNAClean XP (Agencourt Bioscience Corporation, Beverly, Massachusetts) purification. Dye incorporation and cRNA yield were checked using Dropsense™ 96 UV/VIS droplet reader (Trinean, Belgium).

extract_protocol: Total RNA from mouse liver from Erfe-/- mice at t= 0, 24 and 48h hours after phlebotomy was extracted using Trizol (MRC) / Chloroform (Sigma). RNA quality was assessed on RNA 6000 Nano chips using a 2100 Bioanalyzer (Agilent Technologies).

value: liver

tag: treatment

value: phlebotomy

tag: time

tag: gender

value: Male

value: Erfe-/-


title: Knockout IMP2 HCT116 cells miRNA-seq - replicate 3 [VLC_KO3]

description: MGIEasy miRNA-seq

tag: cell line

tag: cell type

value: colorectal cancer


title: Liver-T24-Rep3

tag: genotype


title: ssI-C3

description:
21610_M-16
Processed data file: 202139520099.igv
850 beadchip

tag: integration of cpg free insert

tag: dna methylation at target cgi


title: OS-14

value: parosteal


title: Liver-T0-Rep4

tag: tissue

value: control


title: ssI-C2

description:
21609_M-15
Processed data file: 202139520099.igv
850 beadchip

value: pluripotent stem cell line


title: OS-16


title: OS-25

value: sarcoma pleomorphic with osteoid


title: OS-17


title: OS-13


title: Liver-T24-Rep4


title: ssI-C1

description:
21608_M-6
Processed data file: 202139520099.igv
850 beadchip


title: OS-15


title: OS-11


title: Liver-T48-Rep3


title: Knockout IMP2 HCT116 cells miRNA-seq - replicate 4 [VLC_KO4]

description: MGIEasy miRNA-seq

value: KO IMP2


title: PI-C3

description:
21607_P-20
Processed data file: 202139520099.igv
850 beadchip

source_name: H1 hESC clone with plasmid insert at MLH1 promoter CGI


title: Wild type HCT116 cells miRNA-seq - replicate 1 [VLC_WT1]

description: MGIEasy miRNA-seq


title: OS-10


title: Wild type HCT116 cells miRNA-seq - replicate 3 [VLC_WT3]

description: MGIEasy miRNA-seq


title: Liver-T24-Rep2


title: PI-C1

description:
21605_P-6
Processed data file: 202139520099.igv
850 beadchip


title: PI-C2

description:
21606_P-16
Processed data file: 202139520099.igv
850 beadchip


title: OS-8

value: telangectasic


title: Wild type HCT116 cells miRNA-seq - replicate 2 [VLC_WT2]

description: MGIEasy miRNA-seq


title: H1 Control

description:
21604_H-1
Processed data file: 202139520099.igv
850 beadchip

value: N


title: OS-7

value: male


title: Wild type HCT116 cells miRNA-seq - replicate 4 [VLC_WT4]

description: MGIEasy miRNA-seq

value: WT


title: tail, 28 DPA, regenerated tail portion

status: Public on Jun 20 2023

city: Los Angeles

first: Thomas

middle: P

last: Lozito

address: 1450 Biggy St NRT4513

department: Orthopaedic Surgery

institute: University of Southern California

description: 10x Genomics

data_processing:
Demultiplexing, barcode processing, feature counting and aggregation was performed using Cell Ranger pipeline (10x Genomics, v3.1.2)
Assembly: Green anole (A. carolinensis) genome FASTA and GTF files (v2.105) were used to generate a reference genome for indexing by Cell Ranger mkref function (v3.1.2, 10x Genomics). The reference genome FASTA and GTF files were sourced from the Ensembl database for green anole, AnoCar2.0v2 (GCA_000090745.2), ensembl release 105. (http://dec2021.archive.ensembl.org/Anolis_carolinensis/Info/Index).
Supplementary files format and content: Tab-separated values files and matrices

organism: Anolis carolinensis

source_name: tail regenerated tail portion

extract_protocol:
Lizard tails were anesthetized by exposing tails to a spray of ethyl chloride for 10 seconds and amputated with a sterile scalpel blade to begin regeneration. Tail samples were collected in Hank’s balanced salt solution (HBSS) supplemented with 100 units/mL penicillin and 100 µg/mL streptomycin (HBSS with P/S). Samples were collected at 0, 1, 3, 7, 14, 21, and 28 days after initial amputation or days post-amputation (DPA) for cell dissociation. Longer tail samples (14, 21 and 28 DPA) were further split into two portions at the original amputation plane using a sterile scalpel. Three tail samples per time point were cut to 3-5 mm pieces in length and each piece was cut into 1/8ths. Tail pieces were added to gentleMACS™ C tubes (Miltenyi Biotec, PN: 130-093-237) containing 2.5 mL Dulbecco’s modified Eagle media (DMEM), 100 µL proprietary Enzyme D, 50 µL Enzyme R and 12.5 µL Enzyme A from Multi Tissue Dissociation Kit 1 (Miltenyi Biotec, PN: 130-110-201). Tubes were inverted and placed onto gentleMACS™ OctoDissociator with sleeve (Miltenyi Biotec, PN: 130-096-427) and a fibroblast dissociation protocol was run for 1 hour. Enzymatic activity was inactivated with DMEM supplemented with 10% fetal bovine serum (FBS). Cells were gently resuspended via pipetting and run through MACS® 70 µm SmartStrainer (Miltenyi Biotec, PN: 130-098-462), followed by filtration via Scienceware FlowMi® 40 µM Cell Strainers for p1000 pipettes (Sigma Aldrich, PN: H13680-0040). Cells were pelleted and washed with DMEM with 10% FBS. Cells were resuspended in HBSS with 0.04% bovine serum albumin (BSA) prior to library preparation.
Isolated cells were counted on a hemocytometer and prepared using the 10x Genomics Chromium Single-Cell Gene Expression kit (V2, PN: 120267) according to manufacturer’s recommendations. Cells were encapsulated into droplets via gel bead-in emulsion (GEM) method for barcoding using the 10x Genomics Chromium controller (PN: 1000202). GEMs were incubated for cDNA synthesis, followed by amplification and library construction, according to manufacturer’s instructions.

value: tail (regenerated tail portion)

tag: age

value: 9-12 months old

value: 28 days post amputation


title: tail, 3 DPA

description: 10x Genomics

source_name: tail

value: tail

value: 3 days post amputation


title: tail, 21 DPA, regenerated tail portion

description: 10x Genomics

value: 21 days post amputation


title: tail, 28 DPA, original tail portion

description: 10x Genomics

source_name: tail original tail portion

value: tail (original tail portion)


title: tail, 14 DPA, original tail portion

description: 10x Genomics

value: 14 days post amputation


title: OS-12

value: osteoblastic

tag: Sex

value: female


title: tail, 21 DPA, original tail portion

description: 10x Genomics


title: tail, 14 DPA, regenerated tail portion

description: 10x Genomics


title: OS-9


title: tail, 7 DPA

description: 10x Genomics

value: 7 days post amputation


title: Liver-T48-Rep2

value: liver

value: phlebotomy

value: Male

value: Erfe-/-


title: Liver-T0-Rep3

value: control


title: OS-20

value: male


title: OS-5


title: Liver-T24-Rep1


title: tail, 0 DPA

description: 10x Genomics

value: 0 days post amputation


title: tail, 1 DPA

description: 10x Genomics

value: 9-12 months old

value: 1 day post amputation


title: Liver-T48-Rep1


title: Root-Rep3-aoc-Salt-72h

city: Strasbourg

first: Thierry

last: HEITZ

address: 12 rue du General Zimmer

department: IBMP

institute: CNRS

data_processing:
Raw data were first processed through fastp then filtered by removing reads containing adapters, reads containing N > 10% (N = base that could not be determined), low quality reads (Qscore of over 50% bases of the read)
Mapping to the Os-Nipponbare-Reference-IRGSP-1.0 reference genome with Hisat2
We estimate gene expression level by the abundance of transcripts(count of sequencing) that mapped to genome or exon. Reads count is proportional to gene expression level, gene length and sequencing.
Differentially expression analysis was performed with DESeq2 (package R)
Assembly: Os-Nipponbare-Reference-IRGSP-1.0
Supplementary files format and content: csv files with differentially expressed genes between the different conditions

organism: Oryza sativa

source_name: root

growth_protocol: Plants were grown in hydroponic system at 28°C,  12h/12h photoperiod, with 60% relative humidity

extract_protocol:
Root total RNA was extracted with Rneasy Plant Mini Kit  (Quiagen) and 2nd leaf total RNA extracted with Tri-Reagent. After DNAse treatment, RNA was purified using NucleoSpin RNA Clean-up kit (Macherey-Nagel).
After analysis with an Agilent 2 100 Bioanalyzer, all samples had a RIN value ≥ 6.
illumina PE150 platform, performed by NOVOGENE (Cambridge, UK)

treatment_protocol: Plants were either non-treated or exposed to 100 mM NaCl solution. 3 plants were used per biological replicate.

value: aoc

value: Root


title: Root-Rep3-aoc-Salt-6h


title: Liver-T0-Rep2


title: OS-6

value: telangectasic


title: Liver-T0-Rep1


title: Root-Rep3-aoc-Control-0h


title: Root-Rep3-aoc-Salt-1h


title: Root-Rep3-WT-Salt-72h


title: Root-Rep3-WT-Salt-6h


title: Root-Rep3-WT-Control-0h


title: Root-Rep3-WT-Salt-1h


title: Root-Rep2-aoc-Salt-72h


title: Root-Rep2-aoc-Salt-6h


title: Root-Rep2-WT-Salt-72h


title: Root-Rep1-aoc-Salt-72h


title: Root-Rep2-aoc-Control-0h


title: Root-Rep2-WT-Salt-6h


title: Root-Rep2-WT-Control-0h


title: Root-Rep2-aoc-Salt-1h


title: Root-Rep1-aoc-Salt-1h


title: Root-Rep1-aoc-Control-0h


title: Root-Rep1-WT-Salt-72h


title: Root-Rep1-WT-Salt-1h


title: Root-Rep1-WT-Salt-6h


title: Root-Rep1-aoc-Salt-6h


title: Root-Rep2-WT-Salt-1h


title: OS-2

value: condroblastic


title: Shoot-Rep3-aoc-Salt-72h

source_name: Shoot

value: 2nd leaf


title: Shoot-Rep3-aoc-Salt-6h


title: Root-Rep1-WT-Control-0h


title: Shoot-Rep3-aoc-Salt-1h


title: OS-1

value: osteblastic, condroblastic


title: OS-3


title: Shoot-Rep3-aoc-Control-0h


title: OS-4

value: osteoblastic

value: female


title: Shoot-Rep3-WT-Salt-1h


title: Shoot-Rep3-WT-Salt-6h


title: Shoot-Rep2-aoc-Salt-6h


title: Shoot-Rep2-aoc-Control-0h


title: Shoot-Rep3-WT-Control-0h


title: Shoot-Rep2-WT-Salt-6h


title: Shoot-Rep2-WT-Control-0h


title: Shoot-Rep1-aoc-Salt-6h


title: Shoot-Rep1-aoc-Salt-72h


title: Shoot-Rep2-WT-Salt-72h


title: Shoot-Rep3-WT-Salt-72h


title: Shoot-Rep1-aoc-Control-0h


title: Shoot-Rep2-aoc-Salt-72h


title: Shoot-Rep1-WT-Salt-1h


title: Shoot-Rep1-aoc-Salt-1h


title: Shoot-Rep1-WT-Control-0h


title: Shoot-Rep1-WT-Salt-6h


title: Shoot-Rep2-aoc-Salt-1h


title: eepR-3

city: Pittsburgh

first: Robert

middle: MQ

last: Shanks

state: PA

address: EEI 1020, 203 Lothrop st.

department: Ophthalmology

institute: University of Pittsburgh

data_processing:
sequence reads were trimmed to remove adaptor sequence using Trimmomatic v.0.36.
Trim reads were mapped to Db11 (Accession no. HG326223.1) and K904 ORFs using Bowtie2 aligner v.2.2.6. BAM files were generated.
Unique read hit counts were caluclated using featureCounts from Subread package v.1.5.2.
Differential gene expression was preformed using DESeq2. The Wald test was used to generate p-values and log2 fold changes.
Genome_build: Db11 (Accession no. HG326223.1) and K904
Supplementary_files_format_and_content: tab-delimited text file includes log2 counts per million (CPM) values for each Sample ...

organism: Serratia marcescens

source_name: bacterial RNA

growth_protocol: Cultures were grown to OD=1.0 in Lysogeny Broth

extract_protocol:
RNA later Qiagen; RT-PCR
RNA was isolated using the RNeasy Mini Kit (Qiagen), treated twice with Dnase I, and concentrated with a spin concentrator (Monarch, New England Biolabs).
Lbraries were made by GeneWiz, Steps include: ribosomal RNA depletion, RNA fragmentation and random priming, first and second strand cDNA synthesis, end repair, 5' phosphorylation and dA-tailing, adaptator ligation, PCR enrichment, and sequencing

treatment_protocol: 400µl aliquots were treated with RNAprotect and frozen at -80˚C

tag: background strain

value: Serratia marscecens K904

value: eepR mutant


title: eepR-4


title: Shoot-Rep2-WT-Salt-1h


title: eepR-1


title: eepR-2


title: WT-1

value: wild-type


title: WT-3


title: WT-4


title: Shoot-Rep1-WT-Salt-72h


title: genomic DNA of sample F003_CD15

city: Research Triangle Park

first: Xuting

last: Wang

state: NC

address: 111 TW Alexander Dr

institute: NIEHS/NIH

description: Non-smoker CD15 genomic DNA

hyb_protocol: Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 and MethylationEPIC Beadchips using standard Illumina protocol

scan_protocol: Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting

data_processing: The raw IDAT files of 450K and EPIC methylation arrays were read into R with the minfi package separately; the combineArrays function in minfi was utilized to combine the two arrays’ data together based on their common CpG sites. Then, the data was preprocessed with background and dye bias correction using the preprocess Noob method. The ChAMP package was used to do BMIQ normalization. The combat function in sva package was used to do batch (“Sample_Plate”) correction on methylation array data. Prior to normalization, DNA methylation data were filtered based on these criteria: any samples having more than 5% probes that failed array QC standards, all CpG probes on the X and Y chromosomes, probes containing SNPs with a minor allele frequency ≥ 1% (in EUR or AFR populations of the 1000 Genomes Project) within 5 nucleotides to the CpG site, and probes failing QC standards. We also removed 43,254 probes reported to hybridize to one or more non-target sites in the genome.

label_protocol: 500 ng of DNA was bisulfite converted using the EZ-DNA Methylation kit (Zymo Research)

extract_protocol: Nucleated DNA from cells was collected after sucrose-induced osmotic lysis of cells followed by phenol-chloroform extraction and DNA was stored in Tris-EDTA buffer at -20°C.

value: NS


title: genomic DNA of sample F031_CD8

description: Smoker CD8 genomic DNA

value: SM


title: genomic DNA of sample F031_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F026_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F031_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F001_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F002_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F031_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F001_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F002_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F002_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F002_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F002_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F001_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F001_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F007_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F001_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F011_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F002_CD14

description: Non-smoker CD14 genomic DNA


title: WT-2


title: genomic DNA of sample F007_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F003_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F007_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F007_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F031_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F003_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F003_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F007_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F003_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F007_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F001_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F003_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F052_WB

description: Smoker WB genomic DNA


title: genomic DNA of sample F047_WB

description: Non-smoker WB genomic DNA


title: genomic DNA of sample F061_WB

description: Smoker WB genomic DNA


title: genomic DNA of sample F075_WB

description: Non-smoker WB genomic DNA


title: genomic DNA of sample F044_WB

description: Non-smoker WB genomic DNA


title: genomic DNA of sample F057_WB

description: Smoker WB genomic DNA


title: genomic DNA of sample F016_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F040_WB

description: Smoker WB genomic DNA


title: genomic DNA of sample F048_WB

description: Smoker WB genomic DNA


title: genomic DNA of sample F017_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F043_WB

description: Non-smoker WB genomic DNA


title: genomic DNA of sample F015_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F016_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F016_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F066_WB

description: Smoker WB genomic DNA


title: genomic DNA of sample F016_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F015_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F053_WB

description: Smoker WB genomic DNA


title: genomic DNA of sample F016_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F016_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F015_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F015_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F015_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F014_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F014_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F014_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F015_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F011_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F011_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F014_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F011_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F001_WB

description: Smoker WB genomic DNA


title: genomic DNA of sample F014_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F014_WB

description: Non-smoker WB genomic DNA


title: genomic DNA of sample F014_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F007_WB

description: Smoker WB genomic DNA


title: genomic DNA of sample F011_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F011_WB

description: Smoker WB genomic DNA


title: genomic DNA of sample F017_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F002_WB

description: Non-smoker WB genomic DNA


title: genomic DNA of sample F017_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F018_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F018_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F017_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F018_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F018_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F017_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F018_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F017_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F061_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F011_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F061_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F057_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F003_WB

description: Non-smoker WB genomic DNA


title: genomic DNA of sample F057_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F061_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F061_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F057_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F057_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F057_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F061_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F057_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F053_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F048_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F053_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F018_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F048_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F053_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F048_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F048_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F047_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F026_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F048_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F026_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F053_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F026_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F025_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F053_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F025_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F025_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F053_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F026_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F025_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F038_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F048_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F038_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F040_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F040_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F040_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F040_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F037_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F038_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F025_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F026_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F038_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F037_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F033_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F037_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F033_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F037_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F038_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F033_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F033_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F033_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F037_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F037_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F033_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F040_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F031_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F075_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F075_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F025_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F038_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F075_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F075_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F075_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F061_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F038_WB

description: Smoker WB genomic DNA


title: genomic DNA of sample F033_WB

description: Non-smoker WB genomic DNA


title: genomic DNA of sample F037_WB

description: Smoker WB genomic DNA


title: genomic DNA of sample F017_WB

description: Non-smoker WB genomic DNA


title: genomic DNA of sample F026_WB

description: Non-smoker WB genomic DNA


title: genomic DNA of sample F036_WB

description: Non-smoker WB genomic DNA


title: genomic DNA of sample F031_WB

description: Smoker WB genomic DNA


title: genomic DNA of sample F018_WB

description: Smoker WB genomic DNA


title: genomic DNA of sample F047_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F047_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F043_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F047_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F043_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F047_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F016_WB

description: Smoker WB genomic DNA


title: genomic DNA of sample F043_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F015_WB

description: Non-smoker WB genomic DNA


title: genomic DNA of sample F043_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F040_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F047_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F009_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F043_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F043_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F075_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F006_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F005_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F009_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F013_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F025_WB

description: Non-smoker WB genomic DNA


title: genomic DNA of sample F009_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F021_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F020_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F020_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F021_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F020_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F021_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F005_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F006_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F019_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F005_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F019_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F006_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F019_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F067_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F066_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F066_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F066_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F065_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F066_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F065_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F066_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F065_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F065_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F066_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F065_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F013_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F052_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F052_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F052_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F052_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F073_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F074_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F052_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F073_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F072_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F073_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F074_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F073_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F073_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F072_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F073_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F069_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F072_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F069_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F072_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F069_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F055_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F069_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F054_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F055_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F054_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F055_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F055_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F069_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F054_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F054_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F055_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F052_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F072_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F072_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F062_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F060_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F062_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F062_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F060_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F062_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F062_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F062_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F060_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F064_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F060_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F064_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F064_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F064_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F064_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F064_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F077_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F076_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F077_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F076_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F076_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F074_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F074_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F054_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F054_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F055_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F076_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F074_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F076_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F056_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F058_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F060_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F074_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F065_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F056_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F056_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F058_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F056_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F058_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F056_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F058_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F058_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F045_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F056_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F076_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F044_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F045_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F045_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F045_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F044_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F044_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F044_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F045_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F036_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F044_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F058_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F036_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F030_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F030_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F036_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F036_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F030_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F030_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F030_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F030_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F049_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F049_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F049_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F044_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F049_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F069_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F049_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F068_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F068_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F067_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F068_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F036_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F067_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F067_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F068_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F068_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F036_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F049_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F148_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F149_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F165_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F148_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F165_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F164_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F164_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F165_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F164_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F165_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F164_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F164_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F163_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F151_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F124_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F151_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F152_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F125_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F077_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F020_WB

description: Smoker WB genomic DNA


title: genomic DNA of sample F149_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F149_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F068_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F149_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F149_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F152_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F125_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F152_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F019_WB

description: Smoker WB genomic DNA


title: genomic DNA of sample F148_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F151_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F164_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F013_WB

description: Non-smoker WB genomic DNA


title: genomic DNA of sample F152_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F149_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F021_WB

description: Non-smoker WB genomic DNA


title: genomic DNA of sample F006_WB

description: Non-smoker WB genomic DNA


title: genomic DNA of sample F127_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F009_WB

description: Smoker WB genomic DNA


title: genomic DNA of sample F067_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F163_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F067_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F151_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F125_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F125_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F124_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F135_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F134_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F134_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F140_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F133_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F133_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F125_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F140_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F140_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F124_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F124_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F141_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F141_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F134_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F125_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F124_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F134_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F140_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F133_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F134_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F140_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F140_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F141_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F133_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F124_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F134_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F133_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F121_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F121_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F120_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F121_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F121_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F121_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F120_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F144_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F120_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F144_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F121_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F142_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F144_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F142_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F144_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F120_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F142_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F144_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F142_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F142_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F141_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F141_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F141_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F158_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F158_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F158_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F158_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F144_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F120_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F158_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F158_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F142_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F138_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F138_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F138_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F138_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F136_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F138_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F135_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F136_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F138_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F135_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F135_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F154_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F135_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F153_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F154_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F136_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F135_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F136_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F153_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F154_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F154_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F153_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F153_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F152_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F153_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F117_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F153_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F115_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F115_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F136_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F136_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F115_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F117_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F117_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F117_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F115_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F117_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F115_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F115_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F148_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F147_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F148_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F147_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F147_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F147_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F130_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F129_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F152_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F129_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F127_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F117_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F129_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F127_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F129_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F127_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F129_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F129_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F127_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F119_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F127_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F118_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F148_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F119_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F147_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F147_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F118_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F118_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F119_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F118_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F119_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F119_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F130_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F118_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F119_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F130_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F130_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F130_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F130_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F168_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F168_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F166_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F166_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F168_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F166_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F168_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F166_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F118_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F165_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F165_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F160_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F160_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F160_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F133_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F171_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F170_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F160_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F171_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F160_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F160_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F171_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F171_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F171_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F171_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F155_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F155_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F155_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F166_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F155_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F163_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F154_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F155_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F162_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F166_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F155_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F162_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F163_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F162_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F163_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F162_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F162_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F162_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F170_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F170_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F170_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F169_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F170_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F169_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F169_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F169_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F086_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F169_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F154_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F086_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F168_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F085_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F168_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F085_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F085_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F086_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F086_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F085_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F084_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F163_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F085_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F085_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F084_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F089_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F090_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F090_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F169_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F090_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F089_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F090_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F089_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F089_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F089_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F087_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F087_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F087_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F087_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F082_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F087_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F087_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F081_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F081_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F086_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F082_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F082_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F084_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F081_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F083_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F083_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F082_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F084_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F081_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F089_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F090_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F083_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F083_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F084_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F105_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F083_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F104_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F105_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F104_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F105_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F081_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F104_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F104_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F081_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F105_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F105_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F104_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F112_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F104_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F103_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F082_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F113_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F083_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F112_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F113_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F113_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F112_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F112_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F112_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F112_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F049_WB

description: Non-smoker WB genomic DNA


title: genomic DNA of sample F113_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F084_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F093_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F096_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F093_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F096_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F113_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F096_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F093_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F096_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F093_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F093_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F113_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F079_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F092_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F079_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F079_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F111_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F098_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F096_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F079_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F079_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F082_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F097_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F098_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F097_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F098_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F097_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F098_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F097_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F098_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F096_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F101_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F101_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F101_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F079_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F101_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F097_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F098_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F097_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F108_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F111_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F108_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F108_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F111_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F111_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F108_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F108_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F102_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F111_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F108_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F111_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F102_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F103_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F102_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F103_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F102_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F102_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F103_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F092_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F103_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F103_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F101_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F030_WB

description: Smoker WB genomic DNA


title: genomic DNA of sample F091_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F092_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F091_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F091_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F092_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F092_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F092_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F091_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F090_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F106_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F091_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F091_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F106_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F106_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F105_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F106_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F106_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F078_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F102_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F106_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F078_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F077_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F078_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F077_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F078_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F078_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F186_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F187_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F186_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F187_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F186_CD8

description: Non-smoker CD8 genomic DNA


title: genomic DNA of sample F186_CD4

description: Non-smoker CD4 genomic DNA


title: genomic DNA of sample F187_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F187_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F186_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F186_CD14

description: Non-smoker CD14 genomic DNA


title: genomic DNA of sample F078_CD15

description: Non-smoker CD15 genomic DNA


title: genomic DNA of sample F185_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F185_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F185_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F185_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F190_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F190_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F189_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F190_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F189_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F189_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F190_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F077_CD19

description: Non-smoker CD19 genomic DNA


title: genomic DNA of sample F187_CD56

description: Non-smoker CD56 genomic DNA


title: genomic DNA of sample F189_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F189_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F189_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F190_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F173_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F173_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F172_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F172_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F173_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F172_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F173_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F185_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F172_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F182_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F172_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F173_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F182_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F181_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F190_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F182_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F182_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F181_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F181_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F175_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F181_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F182_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F175_CD8

description: Smoker CD8 genomic DNA


title: genomic DNA of sample F181_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F175_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F175_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F175_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F175_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F178_CD56

description: Smoker CD56 genomic DNA


title: genomic DNA of sample F182_CD14

description: Smoker CD14 genomic DNA


title: genomic DNA of sample F173_CD4

description: Smoker CD4 genomic DNA


title: genomic DNA of sample F181_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F178_CD19

description: Smoker CD19 genomic DNA


title: genomic DNA of sample F178_CD15

description: Smoker CD15 genomic DNA


title: genomic DNA of sample F178_CD4

description: Smoker CD4 genomic DNA


title: iCMP2

city: Berlin

first: Karsten

last: Jürchott

address: Luisenstraße

department: Rahel Hirsch Center

institute: BIH at Charité

data_processing:
HiSeq Control Software 2.2.58, Base-calling: RTA 1.18.64.0.
Sequence reads were demultiplexed using bcl2fastq2 (version 2.18, Illumina) and fastq file quality was assessed using fastqc (version 0.11.7, Bioinformatics Group at the Babraham Institute). Residual adapter sequences and low-quality reads were trimmed using AdapterRemoval (2.2.2).
Reads were aligned to the mm10 (GRCm38.82) version of the mouse genome using tophat (version 2.1.0) and bowtie2 (version 2.2.5). Counts per gene were calculated as the sum of all mapped reads within a gene region. Raw counts of protein-coding genes were normalised and variance stabilizing transformed using DESeq2 (1.28.1) in R (A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. URL https://www.R-project.org/; 4.0.2).
Genome_build: mm10
Supplementary_files_format_and_content: raw_counts - tab-deliminated file with reads summerized per gene.
Supplementary_files_format_and_content: normalized_data - tab-deliminated file with normalized data of protein-coding genes
Supplementary_files_format_and_content: vst_data - tab-deliminated file with variancestabilising transformed data of protein-coding genes

source_name: Induced cardiomyocyte progenitors

growth_protocol: CFs were purchased from PELOBIOTECH (cat. no. PB-C57-6049). For expansion, CFs were plated into gelatin-coated vessels and cultured in high glucose Dulbecco's modified eagle medium (DMEM) supplemented with 10 % heat-inactivated fetal bovine serum (FBS) and 1 % penicillin/streptomycin (P/S, standard medium) at 37 °C and 5 % CO2 in a humified incubator. All experiments were performed with passage 2–5 cells.

extract_protocol: Total RNA was isolated using the Qiagen RNeasy Mini Kit. Genomic DNA was removed with DNAse I. RNA quality and quantity were assessed on a 2100 Bioanalyzer System using the RNA 6000 Nano Kit. For RNA-seq, poly-(A)-selection was performed with 500 ng total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module.

treatment_protocol: CFs were plated in standard medium into gelatin-coated vessels. The next day, CFs were infected with Gata4, Mef2c, Tbx5, and Mef2c lentiviruses at an MOI of 5 each. After 24 h, standard medium was replaced with cardiac reprogramming medium, composed of low glucose DMEM and M199 (3:1) and supplemented with 10 % FBS and 1 % P/S. On day 3 after lentivirus infection, cells were sub-cultured. From day 4 onwards, cells were subjected to G418 selection. Thereafter, reprogramming medium was changed every other day until day 14. Before RNA isolation and RNA sequencing, iCMPs were purified by fluorescence-activated cell sorting based on Myh6/7 mRNA expression using Cy-5–labeled molecular beacons.

value: heart

tag: strain


title: genomic DNA of sample F178_CD8

description: Smoker CD8 genomic DNA


title: AHT2

source_name: Adult left ventricular heart tissue


title: iCMP1


title: AHT1


title: CF1

source_name: Neonatal cardiac fibroblasts


title: iBMDM, shPGAM5, LPS 4 hours 2

city: Sunnyvale

first: Young

middle: Jun

last: Kang

address: 428 Oakmead Pkwy

institute: Molecular Medicine Research Institute

data_processing:
Raw data (raw reads) of fastq format were firstly processed through in-house perl scripts. In this step, clean data (clean reads) were obtained by removing reads containing adapter, reads containing ploy-N and low-quality reads from raw data.
At the same time, Q20, Q30andGCcontent the clean data were calculated. All the downstream analyses were based on the clean data with high quality
Index of the reference genome was built using Hisat2 v2.0.5 and paired-end clean 2 reads were aligned to the reference genome using Hisat2 v2.0.5.
We selected Hisat2 as the mapping tool for that Hisat2 can generate a database of splice junctions based on the gene model annotation file and thus a better mapping result than other non-splice mapping tools.
Quantification of gene expression level featureCounts v1.5.0-p3 was used to count the reads numbers mapped to each gene.
And then FPKM of each gene was calculated based on the length of the gene and reads count mapped to this gene.
FPKM, expected number of Fragments Per Kilobase of transcript sequence per Millions base pairs sequenced, considers the effect of sequencing depth and gene length for the reads count at the same time, and is currently the most commonly used method for estimating gene expression levels.
Assembly: mm10
Supplementary files format and content: Tab-delimited text file includes FPKM for each sample

source_name: Bone marrow-derived macrophage

growth_protocol: DMEM (high glucose) supplemented with 10% FBS, pyruvate, glutamine and antibiotics.

extract_protocol:
Rneasy mini-prep from Qiagen
Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library.

treatment_protocol: 5x10^5 cells per ml, LPS treatment 100 ng/ml for 4 hours

value: Bone marrow-derived macrophage

value: PGAM5 KD

value: LPS