title: Post-capillary venules, biol rep 1


title: Peritubular capillaries, biol rep 4

status: Public on Jun 30 2023

type: SRA

city: Wien

first: Andreas

middle: B

last: Diendorfer

address: Leberstraße 20

country: Austria

institute: TAmiRNA GmbH

library_source: transcriptomic

data_processing:
Overall quality of the next-generation sequencing data was evaluated automatically and manually with fastQC v0.11.9 (Andrews 2010) and multiQC v1.10 (Ewels et al. 2016).
Reads from all passing samples were adapter trimmed and quality filtered using cutadapt v3.3 (Martin 2011) and filtered for a minimum length of 17nt.
Mapping steps were performed with bowtie v1.3.0 (Langmead et al. 2009) and miRDeep2 v2.0.1.2 (Friedländer et al. 2012), whereas reads were mapped first against the genomic reference GRCm38.p6 provided by Ensembl (Zerbino et al. 2018) allowing for two mismatches and subsequently miRBase v22.1 (Griffiths-Jones 2004), filtered for miRNAs of mmu only, allowing for one mismatch.
Assembly: GRCm39 Assembly GCA_000001635.9; miRBase v22.1
Supplementary files format and content: CSV file containing read counts of miRDeep2 mapped miRNAs

molecule: total RNA

organism: Mus musculus

source_name: Kidney

extract_protocol: Realseq Libaray preperation kit according to manufacturer's specification

tag: tissue

value: Kidney

tag: strain

tag: genotype

value: WT

tag: seks

value: male

tag: laser microdissected

value: Yes

tag: microvascular compartment

value: Peritubular capillaries


title: Post-capillary venules, biol rep 2

value: Post-capillary venules


title: Peritubular capillaries, biol rep 1


title: Glomeruli, biol rep 4

value: Glomeruli


title: Glomeruli, biol rep 3


title: Peritubular capillaries, biol rep 3


title: Glomeruli, biol rep 2


title: Glomeruli, biol rep 1


title: scRNAseq, POA E14.5 WT FlashTagged progenitor 1

city: Geneva

first: Denis

last: Jabaudon

state: Geneva

address: Centre Medical Universitaire, Rue Michel Servet 1

department: Department of Basic Neurosciences

country: Switzerland

institute: Geneva University

description:
ROW01_12
CCHT10_counts.csv
CCHT10_1

library_source: transcriptomic single cell

data_processing:
gene expression counts matrix in csv format containing analyed POA E14.5 WT FlashTagged progenitors  - samples in columns annotated by their position in C1 chip, genes in rows with gene symbol notation
Fastq files were demultiplexed using the  barcode containing read  so can be treated as single-end. Sequenced reads were aligned to the mouse genome using the read-mapping algorithm STAR and duplicated reads were corrected using UMI-tools. Non-ambiguously mapped exonic reads were quantified using GenomicAlignments in IntersectionStrict mode.
Doublet cells or empty wells identified on the Fluidigm C1 plate imaging were excluded. Cells expressing < 1000 genes or <100.000 UMIs or <50.000 mapped reads or > 15% reads from mitochondrial genome were excluded from the analysis. Non-interneuron populations were excluded by clustering.
mm10 v3.0.0.

source_name: embryonic brain POA

extract_protocol:
WT C57BL/6 E14.5 brains  were extracted in ice-cold HBSS 2 hours post FlashTag injection in the lateral ventricles. After sectioning with a Mcllwain tissue chopper, POA was micro-dissected under a stereomicroscope and incubated in 0.05% trypsin at 37°C for 5 minutes. After digestion, FBS was added and cells were mechanically dissociated via pipetting and centrifuged 5 minutes at 300g. Cell pellet was then suspended in 1ml HBSS and filtered using a 70um strainer.
FlastTag+ cells were FAC-sorted on a MoFloAstrios device gated to include only top 5% most bright cells. Sorted cells were captured using integrated fluidic circuits and imaged prior to sequencing.

value: embryonic brain POA

tag: developmental stage

tag: cell type

value: interneuron progenitor

tag: treatment


title: Arterioles, biol rep 4

value: Arterioles


title: Arterioles, biol rep 2


title: Arterioles, biol rep 1


title: GDF10 rep1

city: Hangzhou

first: Chen

last: Wentao

state: Zhejiang

address: No. 866 Yuhangtang Road

country: China

institute: Zhejiang University

data_processing:
Paired-end sequencing of the library was performed on the HiSeq XTen sequencers (Illumina, San Diego, CA). FastQC (version 0.11.2) was used for evaluating the quality of sequenced data. Raw reads were filtered by Trimmomatic (version 0.36). Clean reads were mapped to the reference genome by HISAT2 (version 2.0) with default parameters. RSeQC (version 2.6.1) was used to run statistics on the alignment results. The homogeneity distribution and the genomic structure were checked by Qualimap (version 2.2.1). BEDTools (version 2.26.0) was used for statistical analysis of the gene coverage ratio. Gene expression values of the transcripts were computed by StringTie (version 1.3.3b). The TPM eliminated the influence of gene lengths and sequencing discrepancies to enable direct comparison of gene expression between samples. DESeq2 (version 1.12.4) was used to determine differentially expressed genes (DEGs) between two samples. Genes were considered as significantly differentially expressed if q value < 0.05 and |log2foldchange| > 1.
Assembly: mm10
Supplementary files format and content: Raw counts.txt

extract_protocol:
 Total RNA was extracted from longissimus dorsi using the Total RNA Extractor (Trizol) kit and it was treated with RNase-free DNase I to remove genomic DNA contamination. The quality and  amount of  total RNA were determined by Nanodrop 2000
RNA libraries were prepared for sequencing using standard Illumina protocols

tag: cell line

value: muscle stem cells

value: GDF10 treatment for 6h in vitro


title: vehicle rep1

value: vehicle treatment for 6h in vitro


title: FAPs melatonin rep4

data_processing:
Paired-end sequencing of the library was performed on the HiSeq XTen sequencers (Illumina, San Diego, CA). FastQC (version 0.11.2) was used for evaluating the quality of sequenced data. Raw reads were filtered by Trimmomatic (version 0.36). Clean reads were mapped to the reference genome by HISAT2 (version 2.0) with default parameters. RSeQC (version 2.6.1) was used to run statistics on the alignment results. The homogeneity distribution and the genomic structure were checked by Qualimap (version 2.2.1). BEDTools (version 2.26.0) was used for statistical analysis of the gene coverage ratio. Gene expression values of the transcripts were computed by StringTie (version 1.3.3b). The TPM eliminated the influence of gene lengths and sequencing discrepancies to enable direct comparison of gene expression between samples. DESeq2 (version 1.12.4) was used to determine differentially expressed genes (DEGs) between two samples. Genes were considered as significantly differentially expressed if q value < 0.05 and |log2foldchange| > 1.
Assembly: mm10

source_name: tibialis anterior, gastrocnemius and quadriceps

value: fibro-adipogenic progenitors

tag: Sex

value: melatonin treatment for 24h in vitro


title: Arterioles, biol rep 3


title: GDF10 rep2


title: FAPs melatonin rep2

value: tibialis anterior, gastrocnemius and quadriceps


title: Whole kidney, biol rep 4

value: No

value: Whole kidney


title: FAPs melatonin rep1


title: FAPs vehicle rep4

value: vehicle treatment for 24h in vitro


title: FAPs vehicle rep1

value: male


title: Glycerol+Melatonin rep3

source_name: Tibialis anterior muscle

value: Tibialis anterior muscle

tag: timepoint

value: 14 days post glycerol injury

value: glycerol injection+Melatonin


title: FAPs vehicle rep2


title: Peritubular capillaries, biol rep 2

value: Kidney

value: WT

value: Yes

value: Peritubular capillaries


title: Glycerol+Melatonin rep2


title: Glycerol+Melatonin rep1


title: T4.LP.LCMV.d21.SI#4  [D21_LPL_4]

city: La Jolla

first: Ananda

last: Goldrath

state: CA

address: 9500 Gilman Dr

country: USA

institute: UCSD

description:
D21_LPL_4
Small Intestine Lamina Propria, D21 LCMV-Armstrong infection of CD4+ SMARTA cells

data_processing:
Bowtie2
STAR featurecount
DESeq2
Genome_build: UCSC mm10
Supplementary_files_format_and_content: csv of counts from featurecounts filtered for differentially expressed genes from DESeq2

source_name: D21 SI LPL SMARTA

extract_protocol:
Total RNA was extracted in TRIZoL reagent
PrepX RNA-seq library preperation kit (Integenex)

tag: age

value: 6-16 Weeks

value: Small Intestine Lamina Propria

tag: time point

tag: agent

value: LCMV-Armstrong


title: vehicle rep2

value: muscle stem cells

value: vehicle treatment for 6h in vitro


title: T4.LP.LCMV.d21.SI#3  [D21_LPL_3]

description:
D21_LPL_3
Small Intestine Lamina Propria, D21 LCMV-Armstrong infection of CD4+ SMARTA cells


title: T4.IEL.LCMV.d21.SI#4  [D21_IEL_4]

description:
D21_IEL_4
Small Intestine Epithelium, D21 LCMV-Armstrong infection of CD4+ SMARTA cells

source_name: D21 SI IEL SMARTA

value: Small Intestine Epithelium


title: T4.Th1.LCMV.d21.Sp#2  [D21_Th1_2]

description:
D21_Th1_2
Spleen, D21 LCMV-Armstrong infection of CD4+ SMARTA cells

source_name: D21 Spleen Th1 SMARTA

value: Spleen


title: Glycerol rep2

value: glycerol injection+Control


title: Glycerol rep1


title: T4.Th1.LCMV.d21.Sp#1  [D21_Th1_1]

description:
D21_Th1_1
Spleen, D21 LCMV-Armstrong infection of CD4+ SMARTA cells


title: T4.Tfh.LCMV.d21.Sp#4 [D21_Tfh_4]

description:
D21_Tfh_4
Spleen, D21 LCMV-Armstrong infection of CD4+ SMARTA cells

source_name: D21 Spleen Tfh SMARTA


title: FAPs melatonin rep3

value: tibialis anterior, gastrocnemius and quadriceps

value: fibro-adipogenic progenitors

value: melatonin treatment for 24h in vitro


title: T4.Tfh.LCMV.d21.Sp#3  [D21_Tfh_3]

description:
D21_Tfh_3
Spleen, D21 LCMV-Armstrong infection of CD4+ SMARTA cells


title: T4.LP.LCMV.d7.SI#4  [D7_LPL_4]

description:
D7_LPL_4
Small Intestine Lamina Propria, D7 LCMV-Armstrong infection of CD4+ SMARTA cells

source_name: D7 SI LPL SMARTA


title: T4.IEL.LCMV.d21.SI#1  [D21_IEL_1]

description:
D21_IEL_1
Small Intestine Epithelium, D21 LCMV-Armstrong infection of CD4+ SMARTA cells


title: T4.Tfh.LCMV.d21.Sp#2  [D21_Tfh_2]

description:
D21_Tfh_2
Spleen, D21 LCMV-Armstrong infection of CD4+ SMARTA cells


title: GlyceroI rep3

value: Tibialis anterior muscle

value: male

value: 14 days post glycerol injury


title: T4.IEL.LCMV.d21.SI#3  [D21_IEL_3]

description:
D21_IEL_3
Small Intestine Epithelium, D21 LCMV-Armstrong infection of CD4+ SMARTA cells


title: T4.IEL.LCMV.d7.SI#5  [D7_IEL_5]

description:
D7_IEL_5
Small Intestine Epithelium, D7 LCMV-Armstrong infection of CD4+ SMARTA cells

source_name: D7 SI IEL SMARTA


title: T4.LP.LCMV.d7.SI#3  [D7_LPL_3]

description:
D7_LPL_3
Small Intestine Lamina Propria, D7 LCMV-Armstrong infection of CD4+ SMARTA cells


title: T4.IEL.LCMV.d7.SI#4  [D7_IEL_4]

description:
D7_IEL_4
Small Intestine Epithelium, D7 LCMV-Armstrong infection of CD4+ SMARTA cells


title: T4.Th1.LCMV.d7.Sp#5  [D7_Th1_5]

description:
D7_Th1_5
Spleen, D7 LCMV-Armstrong infection of CD4+ SMARTA cells

source_name: D7 Spleen Th1 SMARTA


title: T4.IEL.LCMV.d7.SI#3  [D7_IEL_3]

description:
D7_IEL_3
Small Intestine Epithelium, D7 LCMV-Armstrong infection of CD4+ SMARTA cells


title: FAPs vehicle rep3

value: vehicle treatment for 24h in vitro


title: T4.Th1.LCMV.d7.Sp#1  [D7_Th1_1]

description:
D7_Th1_1
Spleen, D7 LCMV-Armstrong infection of CD4+ SMARTA cells


title: T4.Th1.LCMV.d7.Sp#4 [D7_Th1_4]

description:
D7_Th1_4
Spleen, D7 LCMV-Armstrong infection of CD4+ SMARTA cells


title: T4.Th1.LCMV.d7.Sp#2  [D7_Th1_2]

description:
D7_Th1_2
Spleen, D7 LCMV-Armstrong infection of CD4+ SMARTA cells


title: T4.Tfh.LCMV.d7.Sp#5  [D7_Tfh_5]

description:
D7_Tfh_5
Spleen, D7 LCMV-Armstrong infection of CD4+ SMARTA cells

source_name: D7 Spleen Tfh SMARTA


title: T4.Tfh.LCMV.d7.Sp#4  [D7_Tfh_4]

description:
D7_Tfh_4
Spleen, D7 LCMV-Armstrong infection of CD4+ SMARTA cells


title: T4.Tfh.LCMV.d7.Sp#3  [D7_Tfh_3]

description:
D7_Tfh_3
Spleen, D7 LCMV-Armstrong infection of CD4+ SMARTA cells


title: T4.Tfh.LCMV.d7.Sp#1  [D7_Tfh_1]

description:
D7_Tfh_1
Spleen, D7 LCMV-Armstrong infection of CD4+ SMARTA cells


title: T4.LP.LCMV.d7.SI#5  [D7_LPL_5]

description:
D7_LPL_5
Small Intestine Lamina Propria, D7 LCMV-Armstrong infection of CD4+ SMARTA cells


title: D7_Th1_Rep1

library_source: genomic

data_processing:
ENCODE pipeline
Genome_build: mm10
Supplementary_files_format_and_content: bigBed

molecule: genomic DNA

source_name: D7 Spleen SMARTA

extract_protocol:
Single cell suspensions from the spleen or epithelium of the small intestine were sorted by FACS.
The ATAC-Seq library was generated by Nextera sample prep kit (Illumina).

value: LCMV-Armstronng

value: CD4+ WT SMARTA


title: D20_Th1_Rep2

source_name: D20 Spleen SMARTA

value: 6-16 Weeks


title: D20_Th1_Rep1


title: D7_Th1_Rep2


title: T4.TN.Sp#1 [TN_1]

description:
TN_1
Spleen, Naive

source_name: Naive SMARTA


title: T4.TN.Sp#2 [TN_5]

description:
TN_5
Spleen, Naive


title: D7_IEL_Rep2

source_name: D7 IEL SMARTA


title: D7_IEL_Rep1


title: D20_Tfh_Rep1


title: D7_Tfh_Rep1


title: Bcl6KO_SPL_2

data_processing:
Bowtie2
STAR featurecount
DESeq2
Genome_build: UCSC mm10
Supplementary_files_format_and_content: csv of counts data from featurecount filtered for differentially expressed genes identified through DESeq2

source_name: D7 Spleen Bcl6KO SMARTA

value: LCMV


title: D20_Tfh_Rep2


title: Bcl6KO_IEL_2

source_name: D7 SI IEL Bcl6KO SMARTA


title: D7_LPL_Rep1

source_name: D7 LPL SMARTA

value: Small Intestine Lamina Propria


title: Bcl6KO_IEL_1


title: D7_LPL_Rep2


title: Bcl6KO_LPL_2

source_name: D7 SI LPL Bcl6KO SMARTA


title: Bcl6KO_SPL_1


title: Bcl6KO_LPL_1


title: P2_E01_540_mono_62

city: Edinburgh

first: Nizar

middle: N.

last: Batada

address: Crew Road

country: United Kingdom

institute: Institute for Genetics and Molecular Medicine, University of Edinburgh

data_processing:
Smart-Seq2
Genome_build: mm10
Supplementary_files_format_and_content: counts

molecule: polyA RNA

source_name: peripheral blood

growth_protocol: Cells were isolated from B6Tg(MMTV-PyMT)634Mul/Lellj mice bearing late stage tumors (upto 24mm diameter) or from aged matched  C57BL/6JCrl controls

extract_protocol:
Cells were sorted into 96 well place containing 2 ul of lysis buffer in each well
as per Smart-seq2 protocol (Picelli et al 2013)
Nextera; 75bp + 75bp; HiSeq2500

treatment_protocol: 40µL of blood containing x µl 0.5M EDTA was lysed 400µL RBC lysis buffer (Biolegend), cells washed in excess 10% FCS/PBS, and lysis repeated. Cells were then blocked with 20 µL block and stained with antibodies in 30µL FACS buffer and individual live, single CD11b+ CD45+ CD115+ CD3- CD19-NK1.1- cells were FACS sorted into 96 well plates containing 2 ul of the lysis buffer (0.2% Triton X100 containing 1:20 RNASE inhibitor) per well.

value: monocytes

value: CTRL

value: peripheral blood


title: P2_E01_540_mono_63


title: P2_E01_540_mono_64


title: P2_E01_540_mono_61


title: P2_E01_540_mono_60


title: P2_E01_540_mono_59


title: P2_E01_540_mono_58


title: P2_E01_540_mono_57


title: D7_Tfh_Rep2

value: Spleen


title: P2_E01_540_mono_56


title: P2_E01_540_mono_55


title: P2_E01_540_mono_52


title: P2_E01_540_mono_54


title: P2_E01_540_mono_50


title: P2_E01_540_mono_51


title: P2_E01_540_mono_53


title: P2_E01_540_mono_42


title: P2_E01_540_mono_47


title: P2_E01_540_mono_48


title: P2_E01_540_mono_43


title: P2_E01_540_mono_49


title: P2_E01_540_mono_41


title: P2_E01_540_mono_46


title: P2_E01_540_mono_44


title: P2_E01_540_mono_40


title: P2_E01_540_mono_39


title: P2_E01_540_mono_45


title: P2_E01_540_mono_37


title: P2_E01_540_mono_38


title: P2_E01_540_mono_36


title: P2_E01_540_mono_35


title: P2_E01_540_mono_34


title: P2_E01_540_mono_33


title: P2_E01_540_mono_32


title: P2_E01_540_mono_29


title: P2_E01_540_mono_31


title: P2_E01_540_mono_30


title: P2_E01_540_mono_28


title: P2_E01_540_mono_27


title: P2_E01_540_mono_26


title: P2_E01_540_mono_23


title: P2_E01_540_mono_25


title: P2_E01_540_mono_24


title: P2_E01_540_mono_19


title: P2_E01_540_mono_20


title: P2_E01_540_mono_18


title: P2_E01_540_mono_21


title: P2_E01_540_mono_17


title: P2_E01_540_mono_15


title: P2_E01_540_mono_22


title: P2_E01_540_mono_16


title: P2_E01_540_mono_13


title: P2_E01_540_mono_14


title: P2_E01_540_mono_10


title: L2C8

city: Berkeley

first: Lin

last: He

address: Li Ka Shing Center

department: Dept of Molecular & Cell Biology

institute: University of California, Berkeley

data_processing:
Trimming with trimmomatic-0.39
Alignement with STAR
Quantification with featureCounts
Genome_build: mm10
Supplementary_files_format_and_content: Count matrix in text format
Supplementary_files_format_and_content: SingleCellExperiment Object saved in rds format

source_name: 36hrs post fertilization

growth_protocol: Embryos were dissociated from cumulus cells using hyaluronidase in M2 medium (MR-051-F, millipore Sigma) cultured in KSOM (MR-121-D, millipore Sigma)

extract_protocol:
SMART-Seq v4
Nextera XT DNA library prep kit

treatment_protocol: No treatment was done on the embryos

value: 36hrs post fertilization


title: MOR4

source_name: Approximately 84hrs post fertilization

value: Approximately 84hrs post fertilization


title: MOR7


title: MOR3


title: MOR2


title: MOR6


title: P2_E01_540_mono_12


title: P2_E01_540_mono_09


title: L2C7


title: P2_E01_540_mono_11


title: MOR5


title: MOR8


title: P2_E01_540_mono_07


title: L2C5


title: P2_E01_540_mono_08


title: L2C6


title: P2_E01_540_mono_06


title: L2C4


title: L2C3


title: L2C2


title: L2C1


title: B12 3b

source_name: 24hrs post fertlization

value: 24hrs post fertlization


title: P2_E01_540_mono_05


title: B12 1b


title: P2_E01_540_mono_03


title: P2_E01_540_mono_04


title: P2_E01_540_mono_02


title: P2_E01_540_mono_01


title: B12-2


title: P2_E01_539_mono_63

value: TUM


title: P2_E01_539_mono_61


title: B11 8b

source_name: 22hrs post fertlization

value: 22hrs post fertlization


title: P2_E01_539_mono_62


title: B12-5


title: P2_E01_539_mono_59


title: B12-1


title: P2_E01_539_mono_58


title: B12-4


title: B11 5b


title: P2_E01_539_mono_60


title: B11 7b


title: B11 6b


title: P2_E01_539_mono_57


title: B11 3b


title: B11 2b


title: P2_E01_539_mono_55


title: P2_E01_539_mono_54


title: B11 1b


title: B11 4


title: P2_E01_539_mono_53


title: B11 3


title: P2_E01_539_mono_64


title: B11 1


title: P2_E01_539_mono_52


title: P2_E01_539_mono_51


title: P2_E01_539_mono_50


title: B10 7b

source_name: 20hrs post fertlization

value: 20hrs post fertlization


title: B10 6b


title: P2_E01_539_mono_49


title: B10 4b


title: P2_E01_539_mono_47


title: B10 2b


title: B9 7b

source_name: 18hrs post fertlization

value: 18hrs post fertlization


title: P2_E01_539_mono_46


title: P2_E01_539_mono_45


title: B10 3b


title: P2_E01_539_mono_48


title: P2_E01_539_mono_56


title: B9 6b


title: P2_E01_539_mono_43


title: P2_E01_539_mono_44


title: B9 3b


title: B9 4b


title: B9 2b


title: P2_E01_539_mono_42


title: P2_E01_539_mono_41


title: P2_E01_539_mono_40


title: B9 2


title: B9 3


title: B9 4


title: B9 1


title: P2_E01_539_mono_38


title: P2_E01_539_mono_36


title: B8 6b

source_name: 16hrs post fertlization

value: 16hrs post fertlization


title: B8 5b


title: B8 4b


title: B8 1b


title: B8 5


title: B8 2


title: B8 1


title: B7 5

source_name: 14hrs post fertlization

value: 14hrs post fertlization


title: B7 4


title: P2_E01_539_mono_33


title: P2_E01_539_mono_34


title: P2_E01_539_mono_29


title: B7 2


title: P2_E01_539_mono_39


title: P2_E01_539_mono_28


title: P2_E01_539_mono_31


title: P2_E01_539_mono_35


title: P2_E01_539_mono_25


title: P2_E01_539_mono_26


title: P2_E01_539_mono_30


title: P2_E01_539_mono_27


title: P2_E01_539_mono_37


title: B8 7b


title: B7 1


title: B6 5

source_name: 12hrs post fertlization

value: 12hrs post fertlization


title: P2_E01_539_mono_23


title: P2_E01_539_mono_24


title: B7 3


title: P2_E01_539_mono_22


title: P2_E01_539_mono_32


title: B6 3


title: P2_E01_539_mono_21


title: P2_E01_539_mono_20


title: B6 2


title: B6 1


title: P2_E01_539_mono_19


title: P2_E01_539_mono_18


title: P2_E01_539_mono_17


title: P2_E01_539_mono_15


title: P2_E01_539_mono_16


title: B5 2

source_name: 10hrs post fertilization

value: 10hrs post fertilization


title: B6 4


title: P2_E01_539_mono_12


title: B4 3

source_name: 8hrs post fertlization

value: 8hrs post fertlization


title: B4 2


title: P2_E01_539_mono_13


title: B5 4


title: P2_E01_539_mono_10


title: B4 4


title: P2_E01_539_mono_14


title: B5 3


title: P2_E01_539_mono_11


title: P2_E01_539_mono_08


title: B4 5


title: P2_E01_539_mono_07


title: P2_E01_539_mono_06


title: B4 1


title: B5 5


title: P2_E01_539_mono_04


title: B5 1


title: P2_E01_539_mono_09


title: P2_E01_539_mono_03


title: B2 3

source_name: 2hrs post fertilization

value: 2hrs post fertilization


title: B3b 5

source_name: 6hrs post fertilization

value: 6hrs post fertilization


title: P2_E01_539_mono_01

value: monocytes


title: P2_E01_539_mono_02

value: peripheral blood


title: B2 2


title: P2_E01_532_mono_62

value: CTRL


title: B3b 4


title: B1 5

source_name: 0 hrs post fertilization

value: 0 hrs post fertilization


title: B1 4


title: P2_E01_532_mono_63


title: B1 3


title: B3b 3


title: P2_E01_532_mono_61


title: P2_E01_532_mono_59


title: P2_E01_532_mono_60


title: P2_E01_539_mono_05


title: B3b 1

source_name: 4hrs post fertilization

value: 4hrs post fertilization


title: B3b 2


title: B1 1


title: P2_E01_532_mono_58


title: 8

source_name: Oocyte

value: Oocyte


title: B2 5


title: 7


title: B2 4


title: P2_E01_532_mono_57

source_name: peripheral blood


title: P2_E01_532_mono_64


title: B2 1


title: P2_E01_532_mono_56


title: P2_E01_532_mono_55


title: 6


title: P2_E01_532_mono_54


title: P2_E01_532_mono_53


title: 3


title: 4


title: P2_E01_532_mono_52


title: B1 2


title: 5


title: 1


title: P2_E01_532_mono_50


title: P2_E01_532_mono_48


title: P2_E01_532_mono_47


title: P2_E01_532_mono_45


title: P2_E01_532_mono_44


title: 2S 02262018

type: RNA

city: Houston

first: exRNA

last: BRL

state: TX

address: One Baylor Plaza

department: Molecular & Human Genetics - Milosavljevic

institute: Baylor College of Medicine

description: Healthy Subject

data_processing:
Non-normalized were obtained from (raw CT values)
Normalized were obtained from (substracting the mean of raw CT values by the mean CT values of 3 endogenous controls (miR-511-5p,  27a-5p, and 140-5p))
Short description of the values in the normalized sheet (Delta CT)

label: As per TaqMan Fast Advanced Master Mix Product protocol

organism: Homo sapiens

source_name: Serum

label_protocol: As per TaqMan Advanced miRNA Assays protocol

extract_protocol: RNA was extracted from serum/plasma using Qiagen miRNeasy Serum/Plasma Advanced Kit

value: Male

tag: anticoagulant

value: S

tag: anatomical location

value: Entire cardiovascular system

tag: sample collection method

value: Peripheral venous cannula insertion - forearm


title: 19H 02262018

description: Healthy Subject

source_name: Plasma

value: H


title: 14S 02262018

description: Healthy Subject


title: 10C 02262018

description: Healthy Subject

value: C


title: 12E 02262018

description: Healthy Subject

value: E


title: 5E 02262018

description: Healthy Subject

value: Female


title: 7H 02262018

description: Healthy Subject


title: 17C 02262018

description: Healthy Subject


title: 2

tag: strain


title: P2_E01_532_mono_41

tag: tissue


title: 14H 02262018

description: Healthy Subject


title: 5C 02262018

description: Healthy Subject


title: 19E 02262018

description: Healthy Subject


title: P2_E01_532_mono_42


title: 12C 02262018

description: Healthy Subject


title: P2_E01_532_mono_51


title: P2_E01_532_mono_40


title: P2_E01_532_mono_39


title: P2_E01_532_mono_37


title: 9S 02262018

description: Healthy Subject


title: P2_E01_532_mono_49


title: P2_E01_532_mono_46


title: P2_E01_532_mono_38


title: 2H 02262018

description: Healthy Subject


title: P2_E01_532_mono_36


title: P2_E01_532_mono_35


title: P2_E01_532_mono_43


title: 7E 02262018

description: Healthy Subject


title: P2_E01_532_mono_34


title: 9H 02262018

description: Healthy Subject


title: 19C 02262018

description: Healthy Subject


title: 14E 02262018

description: Healthy Subject


title: 11S 02262018

description: Healthy Subject


title: P2_E01_532_mono_32


title: 2E 02262018

description: Healthy Subject


title: 4S 02262018

description: Healthy Subject


title: P2_E01_532_mono_33


title: P2_E01_532_mono_29


title: 16S 02262018

description: Healthy Subject


title: P2_E01_532_mono_30


title: P2_E01_532_mono_28


title: P2_E01_532_mono_25


title: P2_E01_532_mono_26


title: 14C 02262018

description: Healthy Subject


title: 16H 02262018

description: Healthy Subject


title: 7C 02262018

description: Healthy Subject


title: P2_E01_532_mono_27


title: 18S 02262018

description: Healthy Subject


title: P2_E01_532_mono_24


title: P2_E01_532_mono_23


title: 4H 02262018

description: Healthy Subject


title: P2_E01_532_mono_21


title: 2C 02262018

description: Healthy Subject


title: 16E 02262018

description: Healthy Subject


title: 6S 02262018

description: Healthy Subject


title: P2_E01_532_mono_20


title: P2_E01_532_mono_31


title: 11H 02262018

description: Healthy Subject


title: P2_E01_532_mono_19


title: 9E 02262018

description: Healthy Subject


title: P2_E01_532_mono_18


title: 9C 02262018

description: Healthy Subject


title: 13S 02262018

description: Healthy Subject


title: 4E 02262018

description: Healthy Subject


title: 18H 02262018

description: Healthy Subject


title: P2_E01_532_mono_17


title: P2_E01_532_mono_16


title: P2_E01_532_mono_14


title: P2_E01_532_mono_22


title: 20S 02262018

description: Healthy Subject


title: 1S 02262018

description: Healthy Subject


title: P2_E01_532_mono_15


title: 6H 02262018

description: Healthy Subject


title: P2_E01_532_mono_12


title: P2_E01_532_mono_13


title: P2_E01_532_mono_11


title: 13H 02262018

description: Healthy Subject


title: P2_E01_532_mono_09


title: P2_E01_532_mono_10


title: 11E 02262018

description: Healthy Subject


title: 4C 02262018

description: Healthy Subject


title: P2_E01_532_mono_08


title: P2_E01_532_mono_07


title: 18E 02262018

description: Healthy Subject


title: 8S 02262018

description: Healthy Subject


title: P2_E01_532_mono_06


title: 15S 02262018

description: Healthy Subject


title: P2_E01_532_mono_05


title: 11C 02262018

description: Healthy Subject


title: 6E 02262018

description: Healthy Subject


title: 1H 02262018

description: Healthy Subject


title: 20H 02262018

description: Healthy Subject


title: 16C 02262018

description: Healthy Subject


title: 13E 02262018

description: Healthy Subject


title: P2_E01_532_mono_03


title: P2_E01_531_mono_64


title: P2_E01_532_mono_04


title: 18C 02262018

description: Healthy Subject


title: 3S 02262018

description: Healthy Subject


title: P2_E01_532_mono_01


title: 10S 02262018

description: Healthy Subject


title: 1E 02262018

description: Healthy Subject


title: P2_E01_532_mono_02


title: P2_E01_531_mono_61


title: P2_E01_531_mono_60


title: P2_E01_531_mono_62


title: P2_E01_531_mono_63


title: P2_E01_531_mono_59


title: 15H 02262018

description: Healthy Subject


title: 8H 02262018

description: Healthy Subject


title: P2_E01_531_mono_58


title: P2_E01_531_mono_57


title: 6C 02262018

description: Healthy Subject


title: 20E 02262018

description: Healthy Subject


title: P2_E01_531_mono_56


title: P2_E01_531_mono_55


title: 13C 02262018

description: Healthy Subject


title: P2_E01_531_mono_54


title: 3H 02262018

description: Healthy Subject


title: 8E 02262018

description: Healthy Subject


title: P2_E01_531_mono_53


title: 17S 02262018

description: Healthy Subject


title: P2_E01_531_mono_52


title: 1C 02262018

description: Healthy Subject


title: P2_E01_531_mono_51


title: 10H 02262018

description: Healthy Subject


title: P2_E01_531_mono_50


title: P2_E01_531_mono_49


title: 15E 02262018

description: Healthy Subject


title: 8C 02262018

description: Healthy Subject


title: P2_E01_531_mono_48


title: 3E 02262018

description: Healthy Subject


title: 5S 02262018

description: Healthy Subject


title: 20C 02262018

description: Healthy Subject


title: 12S 02262018

description: Healthy Subject


title: P2_E01_531_mono_46


title: P2_E01_531_mono_45


title: P2_E01_531_mono_43


title: P2_E01_531_mono_47


title: 17H 02262018

description: Healthy Subject


title: P2_E01_531_mono_42


title: P2_E01_531_mono_44


title: 10E 02262018

description: Healthy Subject


title: 15C 02262018

description: Healthy Subject


title: 5H 02262018

description: Healthy Subject


title: P2_E01_531_mono_41


title: P2_E01_531_mono_40


title: 19S 02262018

description: Healthy Subject


title: P2_E01_531_mono_39


title: 17E 02262018

description: Healthy Subject


title: P2_E01_531_mono_38


title: 7S 02262018

description: Healthy Subject


title: ZT22_25C_JRK_Rep2

city: Evanston

first: Ravi

last: Allada

state: Illinois

address: 2200 Campus Drive Room 2-201

institute: Northwestern University

data_processing:
FASTQ files were quantified with kallisto v0.46.2 (single read mode, bootstrap samples n=25)
Obtained transcripts were aggregated to gene level counts with the tximport R library.
Data was quantified as transcripts per million (TPM).
Genome_build: dmel_r6.43
Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every sample
Supplementary_files_format_and_content: Matrix table with TPM counts for every gene and every sample

organism: Drosophila melanogaster

source_name: Fat Body

growth_protocol: All the flies used for experiments were first raised on a standard yeast-cornmeal-molasses based diet under a light-dark (LD) 12:12h cycle. Young mated female (~3 days old) flies (w^{1118}: Bloomington stock number 5905, Clk-Jrk: backcrossed 7 times to w^{1118}) of ~24 hours cohorts were entrained at either 25oC or 18oC for 5 days in 12L:12D cycles on a standard SY (sucrose: 150 g/L, yeast: 150 g/L) diet.

extract_protocol:
To collect dissected fat body samples at 2-hour and 4-hour intervals from ZT0 (set to 8AM) to ZT22 set (set to 10PM) during the daytime only, flies were entrained in two separate incubators with inverse light:dark schedule (LD and DL). On the day of sampling, flies were directly dissected without dry ice to harvest fat tissues in the abdomen. Pinned flies were cut to remove organs in the abdomen (intestine, ovaries, malpighian tubules, etc). Fat tissues attached to epidermis was collected. Dissected fat body tissues from 10 flies were homogenized in pH 7.4 PBS for 2 min using a Kontes motor and pestle, and were incubated TRIzol LS Reagent (Thermo Fisher, Waltham, MA) for 15 min. RNA was extracted according to the manufacturer’s instructions and residual DNA in the homogenized samples was removed by RNAse free DNase I (Thermo Fisher Scientific). Quality of RNA samples were checked with Agilent 2100 Bioanalyzer.
cDNA library was constructed with poly(A) selected mRNA using Truseq RNA library preparation kit and then sequenced at the Genomics Core Facility at the University of Chicago on Illumina HiSeq 2000 System.

value: Fat Body

tag: time


title: ZT22_25C_JRK_Rep1


title: P2_E01_531_mono_37


title: 3C 02262018

description: Healthy Subject


title: 20-2 02052018

description: Healthy Subject

data_processing:
Non-normalized were obtained from (raw CT values)
Normalized were obtained from (substracting the mean of raw CT values by the mean CT values of 3 endogenous controls (miR-30a-5p, 30d-5p, and 30e-5p ))
Short description of the values in the normalized sheet (Delta CT)

extract_protocol: RNA was extracted from plasma using Qiagen miRNeasy Serum/Plasma Advanced Kit


title: P2_E01_531_mono_36


title: 12H 02262018

description: Healthy Subject


title: 19-1 02052018

description: Healthy Subject


title: P2_E01_531_mono_35


title: 19-3 02052018

description: Healthy Subject


title: P2_E01_531_mono_34


title: 19-2 02052018

description: Healthy Subject


title: ZT18_25C_JRK_Rep2


title: 20-3 02052018

description: Healthy Subject


title: ZT18_25C_JRK_Rep1


title: 20-1 02052018

description: Healthy Subject


title: ZT14_25C_JRK_Rep2


title: ZT14_25C_JRK_Rep1


title: P2_E01_531_mono_33


title: 18-3 02052018

description: Healthy Subject


title: 18-2 02052018

description: Healthy Subject


title: 18-1 02052018

description: Healthy Subject


title: 17-3 02052018

description: Healthy Subject


title: ZT10_25C_JRK_Rep2


title: P2_E01_531_mono_32


title: ZT10_25C_JRK_Rep1


title: P2_E01_531_mono_31


title: 17-1 02052018

description: Healthy Subject


title: ZT6_25C_JRK_Rep2


title: 16-3 02052018

description: Healthy Subject


title: 16-2 02052018

description: Healthy Subject


title: 16-1 02052018

description: Healthy Subject


title: P2_E01_531_mono_30


title: 15-3 02052018

description: Healthy Subject


title: ZT6_25C_JRK_Rep1


title: 15-2 02052018

description: Healthy Subject


title: 15-1 02052018

description: Healthy Subject


title: P2_E01_531_mono_29


title: ZT2_25C_JRK_Rep2


title: ZT2_25C_JRK_Rep1


title: P2_E01_531_mono_28


title: 14-2 02052018

description: Healthy Subject


title: 13-3 02052018

description: Healthy Subject


title: 14-1 02052018

description: Healthy Subject


title: P2_E01_531_mono_27


title: ZT24_25C_WT_Rep2


title: 13-2 02052018

description: Healthy Subject


title: ZT24_25C_WT_Rep1


title: P2_E01_531_mono_26


title: 17-2 02052018

description: Healthy Subject


title: P2_E01_531_mono_25


title: 13-1 02052018

description: Healthy Subject


title: 12-3 02052018

description: Healthy Subject


title: ZT22_25C_WT_Rep2


title: P2_E01_531_mono_24


title: 12-2 02052018

description: Healthy Subject


title: ZT22_25C_WT_Rep1


title: 12-1 02052018

description: Healthy Subject


title: P2_E01_531_mono_23


title: 11-3 02052018

description: Healthy Subject


title: 11-2 02052018

description: Healthy Subject


title: ZT20_25C_WT_Rep2


title: 11-1 02052018

description: Healthy Subject


title: 14-3 02052018

description: Healthy Subject


title: P2_E01_531_mono_22


title: 10-3 02052018

description: Healthy Subject


title: ZT20_25C_WT_Rep1


title: P2_E01_531_mono_21


title: 9-2 02052018

description: Healthy Subject


title: 9-1 02052018

description: Healthy Subject


title: 9-3 02052018

description: Healthy Subject


title: ZT18_25C_WT_Rep1


title: ZT18_25C_WT_Rep2


title: 10-2 02052018

description: Healthy Subject


title: ZT16_25C_WT_Rep2


title: P2_E01_531_mono_18


title: P2_E01_531_mono_19


title: 8-2 02052018

description: Healthy Subject


title: ZT16_25C_WT_Rep1


title: 7-3 02052018

description: Healthy Subject


title: 8-1 02052018

description: Healthy Subject


title: P2_E01_531_mono_17


title: 7-2 02052018

description: Healthy Subject


title: ZT14_25C_WT_Rep2


title: P2_E01_531_mono_16


title: 7-1 02052018

description: Healthy Subject


title: 6-3 02052018

description: Healthy Subject


title: 10-1 02052018

description: Healthy Subject


title: 6-2 02052018

description: Healthy Subject


title: P2_E01_531_mono_14


title: P2_E01_531_mono_15


title: 5-3 02052018

description: Healthy Subject


title: 6-1 02052018

description: Healthy Subject


title: ZT14_25C_WT_Rep1


title: ZT12_25C_WT_Rep1


title: 8-3 02052018

description: Healthy Subject


title: 5-2 02052018

description: Healthy Subject


title: ZT10_25C_WT_Rep2


title: 5-1 02052018

description: Healthy Subject


title: P2_E01_531_mono_20


title: P2_E01_531_mono_13


title: P2_E01_531_mono_12


title: ZT12_25C_WT_Rep2


title: 4-1 02052018

description: Healthy Subject


title: ZT10_25C_WT_Rep1


title: 4-2 02052018

description: Healthy Subject


title: P2_E01_531_mono_11


title: 3-1 02052018

description: Healthy Subject


title: 2-3 02052018

description: Healthy Subject


title: P2_E01_531_mono_10


title: P2_E01_531_mono_09


title: ZT8_25C_WT_Rep1


title: 2-2 02052018

description: Healthy Subject


title: 2-1 02052018

description: Healthy Subject


title: ZT6_25C_WT_Rep2


title: 4-3 02052018

description: Healthy Subject


title: P2_E01_531_mono_08


title: 1-3 02052018

description: Healthy Subject


title: 1-2 02052018

description: Healthy Subject


title: 1-1 02052018

description: Healthy Subject


title: ZT6_25C_WT_Rep1


title: ZT4_25C_WT_Rep1


title: ZT4_25C_WT_Rep2


title: P2_E01_531_mono_07


title: ZT8_25C_WT_Rep2


title: ZT2_25C_WT_Rep2


title: YPS128_NaCl_t30min_Rep2

city: Madison

first: Audrey

last: Gasch

state: WI

address: 425G Henry mall

department: Genetics

institute: University of Wisconsin

data_processing: Read counts generated using a script to identify samples from fastq files that are associated with each 10bp index tag

organism: Saccharomyces cerevisiae

growth_protocol: Strains used in the study include BC187, BY4743, NCYC3290, and YPS128. Strains where grown overnight in YPD and subcultured the next morning in 100ml of YPD. Cultures were grown at 30ºC to an OD ~0.6 and collected 15ml of the culture which are the T0 samples (unstressed cells). Then salt was added to the remaining culture to reach a final concentration of 0.7M NaCl. After 30 minutes of growth in salt, another 15ml collection was made which are the T30 samples (cells response to immediate stress). The cultures were grown for an additional 2.5 hours (3 hours total after salt was added) where a final 15ml collection was done representing T3h samples (cells response to continuous stress - recovering). Cell pellets were stored at -80°C.

extract_protocol:
RNA was extracted with hot phenol protocol; RNA was rRNA-depleted with Illumina's Ribo-Zero (Yeast) rRNA Removal Kit
cDNA library was prepared with Illumina's TruSeq Total RNA Stranded Kit

tag: genetic lineage

value: North American

tag: media

value: YPD

tag: stress

value: 0.7M NaCl


title: YPS128_NaCl_t3h_Rep3


title: YPS128_NaCl_t3h_Rep1


title: 3-3 02052018

description: Healthy Subject


title: YPS128_NaCl_t30min_Rep1


title: YPS128_NaCl_t3h_Rep2


title: ZT2_25C_WT_Rep1


title: 3-2 02052018

description: Healthy Subject


title: P2_E01_531_mono_03

tag: cell type

value: monocytes

value: peripheral blood


title: YPS128_NaCl_t0h_Rep1


title: NCYC3290_NaCl_t3h_Rep3

value: West African


title: ZT24_18C_WT_Rep1

tag: treatment


title: NCYC3290_NaCl_t3h_Rep2


title: P2_E01_531_mono_06

value: CTRL


title: NCYC3290_NaCl_t3h_Rep1


title: P2_E01_531_mono_05


title: NCYC3290_NaCl_t30min_Rep3


title: P2_E01_530_mono_64

value: TUM


title: YPS128_NaCl_t0h_Rep3


title: P2_E01_531_mono_04


title: NCYC3290_NaCl_t0h_Rep3


title: NCYC3290_NaCl_t0h_Rep2


title: BC187_NaCl_t3h_Rep3

value: Wine/European


title: NCYC3290_NaCl_t30min_Rep1


title: NCYC3290_NaCl_t0h_Rep1


title: P2_E01_530_mono_62


title: ZT20_18C_WT_Rep1


title: ZT24_18C_WT_Rep2


title: ZT22_18C_WT_Rep1


title: P2_E01_530_mono_63


title: YPS128_NaCl_t0h_Rep2


title: BC187_NaCl_t3h_Rep2


title: P2_E01_530_mono_61


title: BC187_NaCl_t3h_Rep1


title: ZT18_18C_WT_Rep2


title: P2_E01_531_mono_02


title: BC187_NaCl_t30min_Rep3


title: P2_E01_531_mono_01


title: ZT22_18C_WT_Rep2


title: NCYC3290_NaCl_t30min_Rep2


title: BC187_NaCl_t30min_Rep2


title: ZT18_18C_WT_Rep1


title: ZT20_18C_WT_Rep2


title: BC187_NaCl_t0h_Rep3


title: P2_E01_530_mono_59


title: P2_E01_530_mono_60


title: BC187_NaCl_t0h_Rep2


title: P2_E01_530_mono_58


title: ZT16_18C_WT_Rep2


title: BC187_NaCl_t0h_Rep1


title: ZT16_18C_WT_Rep1


title: P2_E01_530_mono_57


title: BY4743_NaCl_t3h_Rep3

value: Laboratory


title: P2_E01_530_mono_56


title: ZT14_18C_WT_Rep2


title: P2_E01_530_mono_55


title: BY4743_NaCl_t30min_Rep2


title: BY4743_NaCl_t30min_Rep1


title: P2_E01_530_mono_54


title: ZT12_18C_WT_Rep2


title: BC187_NaCl_t30min_Rep1


title: ZT12_18C_WT_Rep1


title: BY4743_NaCl_t0h_Rep2


title: BY4743_NaCl_t0h_Rep1


title: P2_E01_530_mono_53


title: Liver_HTJZD_T40_rep6

city: Guangzhou

first: Xiaowen

last: Zhou

state: Guangdong

address: No.232, East Waihuan Road, Guangzhou Higher Education Mega Centre, Panyu District

institute: Guangzhou University of Chinese Medicine

data_processing:
Low quality reads, such as only adaptor, unknown nucleotides>5%, or Q20 <20% (percentage of sequences with sequencing error rates <1%), were removed by perl script.
The clean reads that were filtered from the raw reads were mapped to honeybee (Apis mellifera) genome (OGSv3.2) using Tophat2 (Kim, Pertea et al. 2013) software.
The aligned records from the aligners in BAM/SAM format were further examined to remove potential duplicate molecules.
Gene expression levels were estimated using FPKM values (fragments per kilobase of exon per million fragments mapped) by the Cufflinks software (Trapnell, Williams et al. 2010).
Assembly: Rnor_6.0_release85
Supplementary files format and content: tab-delimited text file include RPKM values for 16 Samples

organism: Rattus norvegicus

source_name: Liver

growth_protocol: Before treatment, the rat model of HLP was established by the fed with high-fat diet for 4 weeks.

extract_protocol:
Total RNA was extracted from liver tissue samples  by using TRIzol reagent.
The cDNA library was constructed following the manufacturer’s instructions of NEB Next Ultra RNA Library Prep Kit for Illumina (NEB, E7530）and NEB Next Multiplex Oligos for Illumina ( NEB, E7500). In briefly, the enriched mRNA was fragmented into approximately 200nt RNA inserts, which were used to synthesize the first-strand cDNA and the second cDNA. The double-stranded cDNA were performed end-repair/dA-tail and adaptor ligation. The suitable fragments were isolated by Agencourt AMPure XP beads (Beckman Coulter, Inc.), and enriched by PCR amplification. Finally, the constructed cDNA libraries of the honey bee were sequenced on a flow cell using an Illumina HiSeq™ sequencing platform.

treatment_protocol: In the treatment phase, the rats in control group (0.9% saline) were fed with normal diet and the rat model were continuously supplied with HFD but regrouped into three groups according to different administration solution: model group (0.9% saline), 12.6g/kg HTJZD group and 4.54g/kg PC-AR group for 4 weeks. Each rat was given an intragastric dose of 1 mL/100g.

value: Liver

tag: group

value: Model

value: HTJZD


title: ZT10_18C_WT_Rep2


title: Liver_HTJZD_T39_rep5


title: Liver_HTJZD_T38_rep4


title: P2_E01_530_mono_52


title: Liver_HTJZD_T36_rep2


title: ZT10_18C_WT_Rep1


title: Liver_HTJZD_T37_rep3


title: ZT8_18C_WT_Rep2


title: P2_E01_530_mono_51


title: BY4743_NaCl_t3h_Rep2


title: ZT8_18C_WT_Rep1


title: BY4743_NaCl_t3h_Rep1


title: BY4743_NaCl_t0h_Rep3


title: ZT14_18C_WT_Rep1


title: P2_E01_530_mono_50


title: Liver_HTJZD_T35_rep1


title: P2_E01_530_mono_49


title: Liver_PC-AR_T32_rep4

value: PC-AR


title: Liver_PC-AR_T33_rep5


title: ZT6_18C_WT_Rep1


title: ZT6_18C_WT_Rep2


title: BY4743_NaCl_t30min_Rep3


title: Liver_PC-AR_T31_rep3


title: Liver_PC-AR_T34_rep6


title: Liver_PC-AR_T30_rep2


title: P2_E01_530_mono_47


title: P2_E01_530_mono_46


title: Liver_Model_T10_rep5

value: saline


title: Liver_Model_T09_rep4


title: ZT2_18C_WT_Rep2


title: Liver_Model_T08_rep3


title: ZT4_18C_WT_Rep2


title: P2_E01_530_mono_45


title: Liver_Model_T07_rep2


title: Liver_Control_T05_rep5

value: Control


title: Liver_Control_T04_rep4


title: Liver_Model_T06_rep1


title: P2_E01_530_mono_44


title: ZT2_18C_WT_Rep1

value: Fat Body


title: P2_E01_530_mono_48


title: Liver_Control_T03_rep3


title: P2_E01_530_mono_43


title: Liver_Control_T02_rep2


title: Liver_PC-AR_T29_rep1


title: P2_E01_530_mono_42


title: P2_E01_530_mono_41


title: scRNAseq, POA E14.5 WT dissection

description:
AAACCTGAGCGTAGTG-1  AAACCTGTCATTCACT-1  AAACCTGTCGCATGGC-1  AAACGGGAGGCAAAGA-1  AAAGATGCAATACGCT-1  AAAGATGCACGGCTAC-1  AAAGATGTCTGTACGA-1  AAAGCAAAGACAGAGA-1  AAAGCAAAGTTACCCA-1  AAAGCAAGTAGCTCCG-1  AAAGCAAGTCGTTGTA-1  AAAGCAATCAGCAACT-1  AAAGCAATCCTTAATC-1  AAAGTAGAGACAGGCT-1  AAAGTAGCATATGCTG-1  AAAGTAGGTCTCAACA-1  AAAGTAGGTTAAGATG-1  AAAGTAGTCAATACCG-1  AAAGTAGTCTGGCGAC-1  AAATGCCAGAGTACAT-1  AAATGCCCACGACGAA-1  AACACGTAGCTAAACA-1  AACACGTCACTTGGAT-1  AACACGTCAGTTCCCT-1  AACACGTCATAAAGGT-1  AACACGTTCTCGTATT-1  AACACGTTCTTGCATT-1  AACCATGAGGAGTACC-1  AACCATGAGGGTATCG-1  AACCATGCAGCTGGCT-1  AACCATGGTCTGCCAG-1  AACCATGTCACCCTCA-1  AACCGCGTCACTCCTG-1  AACCGCGTCGCGCCAA-1  AACCGCGTCTGTCCGT-1  AACGTTGAGACACTAA-1  AACGTTGAGAGCCTAG-1  AACGTTGAGGTGTTAA-1  AACGTTGCAATGGAGC-1  AACGTTGGTCATACTG-1  AACGTTGGTGGTACAG-1  AACGTTGTCACGACTA-1  AACTCAGAGTATCTCG-1  AACTCAGCACCGAATT-1  AACTCAGCATGGTTGT-1  AACTCAGGTAACGTTC-1  AACTCAGTCAATCACG-1  AACTCAGTCGATCCCT-1  AACTCAGTCTCGAGTA-1  AACTCCCGTAGGGTAC-1  AACTCCCGTCTTTCAT-1  AACTCCCTCACCAGGC-1  AACTCTTAGGGATACC-1  AACTCTTCACGTGAGA-1  AACTCTTGTAACGACG-1  AACTCTTTCAATCACG-1  AACTGGTCATCGACGC-1  AACTGGTTCTTGTATC-1  AACTTTCAGCAGGTCA-1  AACTTTCCACTGTTAG-1  AACTTTCCAGCCTATA-1  AACTTTCGTATAAACG-1  AACTTTCGTTAAAGAC-1  AACTTTCTCCTAGGGC-1  AAGACCTAGATGCCTT-1  AAGACCTAGGTGGGTT-1  AAGACCTAGTAGATGT-1  AAGACCTCATCACCCT-1  AAGACCTCATTCCTGC-1  AAGACCTGTCTCTCTG-1  AAGACCTGTGTAAGTA-1  AAGCCGCAGCAACGGT-1  AAGCCGCAGCTGGAAC-1  AAGCCGCCAGTATAAG-1  AAGCCGCTCGCGATCG-1  AAGGAGCAGGTGCTAG-1  AAGGAGCCAACGCACC-1  AAGGAGCCACAGGAGT-1  AAGGAGCCACTCTGTC-1  AAGGAGCCAGCCTATA-1  AAGGAGCGTAGAAAGG-1  AAGGAGCGTCAGATAA-1  AAGGAGCGTTCCACAA-1  AAGGAGCTCACCGTAA-1  AAGGCAGCAGGTGGAT-1  AAGGCAGGTCGCGGTT-1  AAGGCAGGTCGGCACT-1  AAGGTTCCAAGCCCAC-1  AAGGTTCGTAACGCGA-1  AAGGTTCTCCTTCAAT-1  AAGGTTCTCTGCTGCT-1  AAGGTTCTCTTATCTG-1  AAGTCTGTCGCCAAAT-1  AATCCAGAGAAGGGTA-1  AATCCAGCAGTAAGAT-1  AATCCAGTCAGAGACG-1  AATCCAGTCGGAATCT-1  AATCGGTCAATGTAAG-1  AATCGGTTCAACCATG-1  AATCGGTTCTACTTAC-1  ACACCAAAGAATGTTG-1  ACACCAAAGCTAACTC-1  ACACCAACACCACCAG-1  ACACCAACAGGGTACA-1  ACACCAATCAGTTGAC-1  ACACCAATCGGAGCAA-1  ACACCCTAGCGTAGTG-1  ACACCCTGTCTGATTG-1  ACACCCTGTTGGAGGT-1  ACACCCTTCCATGCTC-1  ACACCGGAGTAATCCC-1  ACACCGGCATGTCCTC-1  ACACTGAAGACGCACA-1  ACACTGACATTACGAC-1  ACACTGAGTAGCAAAT-1  ACACTGAGTTGGTGGA-1  ACACTGATCTTTCCTC-1  ACAGCCGAGTCGATAA-1  ACAGCCGCAACACCCG-1  ACAGCCGGTGAGGCTA-1  ACAGCCGTCGCCAAAT-1  ACAGCTACATAGGATA-1  ACAGCTAGTGAAGGCT-1  ACATACGAGGTAGCCA-1  ACATACGCACCTCGGA-1  ACATACGCAGGTCGTC-1  ACATACGTCTATCGCC-1  ACATACGTCTCATTCA-1  ACATCAGCAATGGATA-1  ACATGGTAGCAGGCTA-1  ACATGGTTCACAGGCC-1  ACCAGTAAGGAATGGA-1  ACCAGTACAGATCCAT-1  ACCAGTAGTACTCGCG-1  ACCAGTAGTTCACCTC-1  ACCAGTATCAAGATCC-1  ACCCACTAGCTACCGC-1  ACCCACTAGGAACTGC-1  ACCCACTAGGGTATCG-1  ACCGTAACAAGGTTTC-1  ACCGTAAGTAAGGGCT-1  ACCGTAAGTAGAGCTG-1  ACCGTAAGTCCTAGCG-1  ACCTTTAGTCGCCATG-1  ACCTTTAGTGAACCTT-1  ACCTTTATCCGTTGCT-1  ACCTTTATCTGAGGGA-1  ACGAGCCAGCGATAGC-1  ACGAGCCCAGGAATCG-1  ACGAGCCGTCTGCGGT-1  ACGAGCCGTTAAAGTG-1  ACGAGCCGTTGCCTCT-1  ACGAGGAAGCCACGTC-1  ACGAGGACACTTCGAA-1  ACGAGGACATGACATC-1  ACGAGGAGTACCGAGA-1  ACGAGGAGTAGAGCTG-1  ACGAGGAGTCGGCATC-1  ACGAGGAGTCTGCCAG-1  ACGAGGAGTGCCTGCA-1  ACGAGGATCGGCTTGG-1  ACGATACAGTCCGGTC-1  ACGATACCACGACGAA-1  ACGATACCAGTCGATT-1  ACGATACGTTGGAGGT-1  ACGATACTCGCATGGC-1  ACGATACTCGGACAAG-1  ACGATACTCTACTATC-1  ACGATGTAGTACTTGC-1  ACGATGTGTGCAGGTA-1  ACGATGTGTTACCAGT-1  ACGATGTTCAGGTTCA-1  ACGATGTTCATATCGG-1  ACGATGTTCGTCCGTT-1  ACGATGTTCTTCGGTC-1  ACGCAGCAGCCGGTAA-1  ACGCAGCCAGGATCGA-1  ACGCAGCCAGGGTACA-1  ACGCAGCGTCGTTGTA-1  ACGCAGCTCGCGGATC-1  ACGCCAGAGGAACTGC-1  ACGCCAGAGTCCATAC-1  ACGCCAGAGTGAATTG-1  ACGCCAGCACCTTGTC-1  ACGCCAGGTAACGACG-1  ACGCCAGTCACCTCGT-1  ACGCCAGTCTGTTGAG-1  ACGCCGAAGACGACGT-1  ACGCCGAAGCTAGCCC-1  ACGCCGAAGGCTATCT-1  ACGCCGAGTTGACGTT-1  ACGCCGATCGTACCGG-1  ACGCCGATCTATGTGG-1  ACGGAGAAGTGTCCCG-1  ACGGAGACAGGTGCCT-1  ACGGAGATCTACTATC-1  ACGGCCAAGAGTAATC-1  ACGGCCAAGATCCTGT-1  ACGGCCAAGCGGCTTC-1  ACGGCCACAATTGCTG-1  ACGGCCACACGCCAGT-1  ACGGCCAGTGCACGAA-1  ACGGCCAGTTGGTAAA-1  ACGGGCTAGAGTGAGA-1  ACGGGCTAGGGAACGG-1  ACGGGCTCACTACAGT-1  ACGGGTCAGAATTGTG-1  ACGGGTCAGTGGTAGC-1  ACGGGTCCACATAACC-1  ACGGGTCCATCCTAGA-1  ACGGGTCGTCAAAGCG-1  ACGGGTCGTTACCGAT-1  ACGGGTCTCCGAGCCA-1  ACGTCAAAGGCAGTCA-1  ACGTCAACAGTAACGG-1  ACGTCAACATCCGTGG-1  ACTATCTAGACCCACC-1  ACTATCTAGCTCCTCT-1  ACTATCTCAAGCTGAG-1  ACTATCTGTTTCGCTC-1  ACTGAACAGCCACGTC-1  ACTGAACAGCTCCCAG-1  ACTGAACAGGGCTTCC-1  ACTGAACCACTGTCGG-1  ACTGAACGTACGCACC-1  ACTGAACGTCGGCACT-1  ACTGAACGTGACTACT-1  ACTGAACTCAACACCA-1  ACTGAACTCGCCCTTA-1  ACTGAACTCGTGGACC-1  ACTGAGTCATCACAAC-1  ACTGAGTGTCCGAATT-1  ACTGAGTTCGTGGACC-1  ACTGATGAGCGAAGGG-1  ACTGATGAGGGTCTCC-1  ACTGATGAGTGCTGCC-1  ACTGATGCAATCTACG-1  ACTGATGGTCTCCACT-1  ACTGATGTCCTGTACC-1  ACTGCTCCAATAGCGG-1  ACTGCTCCAATTGCTG-1  ACTGCTCTCATGCTCC-1  ACTGCTCTCCACTGGG-1  ACTGTCCAGGCAATTA-1  ACTGTCCCATACTCTT-1  ACTGTCCTCGTGTAGT-1  ACTGTCCTCTTGTCAT-1  ACTTACTCATGACGGA-1  ACTTACTGTCAGGACA-1  ACTTACTTCTCATTCA-1  ACTTACTTCTCGAGTA-1  ACTTACTTCTGGTATG-1  ACTTGTTAGTGCTGCC-1  ACTTGTTCACGGCCAT-1  ACTTGTTGTAGTGAAT-1  ACTTGTTGTTCGCGAC-1  ACTTTCAAGCAGCGTA-1  ACTTTCATCAAACCGT-1  ACTTTCATCACATAGC-1  ACTTTCATCGGAAACG-1  AGAATAGAGCCTCGTG-1  AGAATAGGTACAGACG-1  AGAATAGGTATGAAAC-1  AGACGTTAGAATCTCC-1  AGACGTTAGGGATGGG-1  AGACGTTCAATGGACG-1  AGACGTTCACACATGT-1  AGACGTTGTCCGAACC-1  AGACGTTTCACGACTA-1  AGAGCGAAGAGTCTGG-1  AGAGCGAGTACTCTCC-1  AGAGCGAGTGTGAAAT-1  AGAGCGATCTGTCTAT-1  AGAGCTTAGAGTCTGG-1  AGAGCTTAGGGTCTCC-1  AGAGCTTCACTATCTT-1  AGAGCTTCATCTGGTA-1  AGAGCTTGTCGCGTGT-1  AGAGTGGAGACCACGA-1  AGAGTGGAGCCATCGC-1  AGAGTGGAGCGTAGTG-1  AGAGTGGAGGCCATAG-1  AGAGTGGCAACTTGAC-1  AGAGTGGCATCTGGTA-1  AGAGTGGGTTCGTCTC-1  AGAGTGGTCAGCCTAA-1  AGATCTGAGAAGGTTT-1  AGATCTGAGGCTCAGA-1  AGATCTGAGTAGTGCG-1  AGATCTGTCAATACCG-1  AGCAGCCAGAAAGTGG-1  AGCAGCCAGATCCCGC-1  AGCAGCCCATGCTAGT-1  AGCAGCCGTCTGATCA-1  AGCAGCCTCACTCTTA-1  AGCAGCCTCATGTGGT-1  AGCAGCCTCTTAACCT-1  AGCATACAGTCACGCC-1  AGCATACCAGACGCAA-1  AGCATACGTTTAGGAA-1  AGCCTAACAAGGACTG-1  AGCCTAACAGGAACGT-1  AGCCTAACAGTAAGAT-1  AGCCTAAGTCTGGAGA-1  AGCCTAAGTGTGACGA-1  AGCCTAATCGTACGGC-1  AGCGGTCCACACTGCG-1  AGCGGTCGTAGCGTCC-1  AGCGGTCTCGGTCCGA-1  AGCGTATAGCGGCTTC-1  AGCGTATAGTCACGCC-1  AGCGTATCAGCGATCC-1  AGCGTATGTCATATCG-1  AGCGTATGTGACTACT-1  AGCGTATTCCTGCAGG-1  AGCGTCGAGCGTGTCC-1  AGCGTCGAGGGATACC-1  AGCGTCGAGGGTTTCT-1  AGCGTCGCAGGGTTAG-1  AGCGTCGGTATGGTTC-1  AGCGTCGGTCATCGGC-1  AGCGTCGTCTCCGGTT-1  AGCTCCTAGGAATTAC-1  AGCTCCTCAAGTCATC-1  AGCTCCTCAGATCCAT-1  AGCTCCTGTCATGCAT-1  AGCTCCTGTCATTAGC-1  AGCTCCTGTTGAACTC-1  AGCTCTCCACTATCTT-1  AGCTCTCGTCTTCTCG-1  AGCTCTCGTTACCGAT-1  AGCTTGAAGAAGGTGA-1  AGCTTGAAGAGAGCTC-1  AGCTTGACACGCTTTC-1  AGCTTGACAGCTGTGC-1  AGCTTGAGTCAGAAGC-1  AGCTTGAGTCATGCAT-1  AGCTTGATCCTCGCAT-1  AGGCCACAGAATGTGT-1  AGGCCACCAGCCACCA-1  AGGCCACCAGTTTACG-1  AGGCCACGTTAAGTAG-1  AGGCCACTCACTTCAT-1  AGGCCACTCAGTGCAT-1  AGGCCGTAGTCAAGCG-1  AGGCCGTCAAGGTTCT-1  AGGCCGTGTACAGTGG-1  AGGCCGTGTGCACCAC-1  AGGCCGTTCCTTTCTC-1  AGGCCGTTCTGCGACG-1  AGGGAGTAGAAAGTGG-1  AGGGAGTGTATTAGCC-1  AGGGAGTTCACTATTC-1  AGGGATGCAAGTTAAG-1  AGGGATGCACCAGATT-1  AGGGATGCAGCCTTTC-1  AGGGATGGTATAGGTA-1  AGGGATGGTCCTCTTG-1  AGGGATGGTCCTGCTT-1  AGGGATGGTGGCCCTA-1  AGGGATGTCTCCTATA-1  AGGGTGACAGACTCGC-1  AGGGTGACAGGACCCT-1  AGGGTGATCCATGAGT-1  AGGTCATAGTGGTAAT-1  AGGTCATCAAGAAAGG-1  AGGTCATCAGGAACGT-1  AGGTCATGTCATTAGC-1  AGGTCATTCTAGAGTC-1  AGGTCCGAGCCAGTTT-1  AGGTCCGGTACCGCTG-1  AGGTCCGGTGATAAGT-1  AGGTCCGTCTTTACAC-1  AGTAGTCAGCACAGGT-1  AGTAGTCGTCAGAAGC-1  AGTAGTCTCCCTGACT-1  AGTCTTTAGATACACA-1  AGTCTTTAGCGCCTCA-1  AGTCTTTCAAGACGTG-1  AGTCTTTCACGTAAGG-1  AGTCTTTCATCGATTG-1  AGTCTTTGTATGCTTG-1  AGTCTTTGTCTAGTGT-1  AGTCTTTGTGCCTGTG-1  AGTCTTTTCTGCAGTA-1  AGTGAGGCAGCCACCA-1  AGTGAGGGTGGACGAT-1  AGTGAGGGTGTGCCTG-1  AGTGAGGTCCTGTAGA-1  AGTGAGGTCGGAGGTA-1  AGTGGGACAAGCCGCT-1  AGTGGGAGTGTCGCTG-1  AGTGGGATCTGTCTAT-1  AGTGTCAAGGACAGAA-1  AGTGTCAGTCGGCTCA-1  AGTGTCAGTCTAGCGC-1  AGTGTCATCCAATGGT-1  AGTTGGTAGCGCTCCA-1  AGTTGGTAGTCTCAAC-1  AGTTGGTGTAAGTTCC-1  ATAACGCAGAAACCAT-1  ATAACGCCACGGACAA-1  ATAACGCCATACAGCT-1  ATAACGCGTAGTGAAT-1  ATAACGCGTGTGAAAT-1  ATAACGCTCAACGGGA-1  ATAAGAGAGCCGATTT-1  ATAAGAGAGGACACCA-1  ATAAGAGCAAGCCGTC-1  ATAAGAGTCAGAGCTT-1  ATAGACCCATGGTAGG-1  ATAGACCCATGGTCAT-1  ATAGACCGTACCGCTG-1  ATAGACCTCTAACTGG-1  ATAGACCTCTTGGGTA-1  ATCACGAAGTTCCACA-1  ATCACGAGTACCCAAT-1  ATCACGATCCTACAGA-1  ATCACGATCTTAGCCC-1  ATCATCTAGCTAAACA-1  ATCATCTCAAGCTGTT-1  ATCATCTGTCCGACGT-1  ATCATCTGTGAACCTT-1  ATCATCTTCCGTTGTC-1  ATCATGGCAAGGCTCC-1  ATCCACCAGAGACTTA-1  ATCCACCAGCTAGGCA-1  ATCCACCCACACAGAG-1  ATCCACCCACCAGGTC-1  ATCCACCCAGATGGGT-1  ATCCACCGTGCGAAAC-1  ATCCACCTCCACTGGG-1  ATCCGAAAGCAGATCG-1  ATCCGAAAGCCAGTTT-1  ATCCGAACAGCTGCTG-1  ATCCGAACATGCCTTC-1  ATCCGAAGTAGAGCTG-1  ATCGAGTAGCTCCCAG-1  ATCGAGTCACGAAATA-1  ATCGAGTGTAGTAGTA-1  ATCGAGTGTGTAAGTA-1  ATCGAGTTCGGTGTCG-1  ATCTACTCATAAAGGT-1  ATCTACTCATCCTAGA-1  ATCTACTGTCACCTAA-1  ATCTACTGTTATGTGC-1  ATCTGCCAGGGCTTGA-1  ATGAGGGGTCTGCGGT-1  ATGCGATAGCTCTCGG-1  ATGCGATCAAGCTGGA-1  ATGCGATTCACATGCA-1  ATGGGAGGTCGCGTGT-1  ATGGGAGTCTGTTGAG-1  ATGTGTGAGGCATGTG-1  ATGTGTGAGTGGTAAT-1  ATGTGTGCACGCCAGT-1  ATTACTCCAAGCTGGA-1  ATTACTCCAGACGTAG-1  ATTACTCGTCGGGTCT-1  ATTACTCGTCTCGTTC-1  ATTACTCTCAAGCCTA-1  ATTATCCAGGCTAGCA-1  ATTATCCCAATGTTGC-1  ATTATCCCACTTAAGC-1  ATTATCCGTTCCGGCA-1  ATTATCCGTTGATTGC-1  ATTATCCTCATCATTC-1  ATTATCCTCATGTCCC-1  ATTCTACAGTTCGCAT-1  ATTCTACCAAAGCAAT-1  ATTCTACCACAGAGGT-1  ATTCTACGTCTTCTCG-1  ATTCTACGTTCAGTAC-1  ATTGGACCACTAGTAC-1  ATTGGACCATGCATGT-1  ATTGGACGTAAGTAGT-1  ATTGGACGTCAACTGT-1  ATTGGACTCATATCGG-1  ATTGGTGCAAGCCATT-1  ATTGGTGCATCAGTCA-1  ATTGGTGGTATAGGGC-1  ATTGGTGTCGCGATCG-1  ATTGGTGTCTCGATGA-1  ATTTCTGAGCGTCTAT-1  ATTTCTGAGTGTCCCG-1  ATTTCTGCAGTTTACG-1  ATTTCTGCATCCTTGC-1  ATTTCTGGTATAGTAG-1  ATTTCTGGTCGACTGC-1  ATTTCTGGTTCCACTC-1  ATTTCTGTCAGTTTGG-1  ATTTCTGTCGTTTATC-1  CAACCAACACAGGCCT-1  CAACCAAGTAGCCTCG-1  CAACCAATCACTTCAT-1  CAACCAATCCATGAGT-1  CAACCAATCGAATGCT-1  CAACCAATCTTAGAGC-1  CAACCTCCACATCCAA-1  CAACCTCGTCCGAGTC-1  CAACCTCGTCCTCCAT-1  CAACCTCGTGATAAGT-1  CAACCTCTCACGCATA-1  CAACCTCTCGTCGTTC-1  CAACTAGAGCCACTAT-1  CAACTAGAGTACGACG-1  CAACTAGAGTGTCCCG-1  CAACTAGCAGTCTTCC-1  CAACTAGCATCACCCT-1  CAACTAGCATGCTAGT-1  CAACTAGGTCATGCCG-1  CAAGAAACACCAGATT-1  CAAGAAAGTCAAACTC-1  CAAGAAATCCATTCTA-1  CAAGAAATCCGCTGTT-1  CAAGATCAGTACTTGC-1  CAAGATCTCTTATCTG-1  CAAGGCCGTCCAACTA-1  CAAGGCCTCTGAGTGT-1  CAAGTTGAGTTCGCGC-1  CAAGTTGGTGCAGGTA-1  CAAGTTGGTGGGTATG-1  CAAGTTGGTGGTCCGT-1  CAAGTTGTCTATCCCG-1  CACAAACAGTGGGTTG-1  CACAAACAGTGTTTGC-1  CACAAACGTGTGACGA-1  CACAAACTCCATTCTA-1  CACAAACTCGTGGACC-1  CACACAAAGAAGGTTT-1  CACACAAAGATGGGTC-1  CACACAACAATAACGA-1  CACACAACACTCTGTC-1  CACACAACAGATGGGT-1  CACACAACAGCCAGAA-1  CACACAAGTCGCTTCT-1  CACACAATCGCAAACT-1  CACACCTCAGTTTACG-1  CACACCTGTGTGTGCC-1  CACACCTTCAAAGTAG-1  CACACTCAGAGATGAG-1  CACACTCAGTCATCCA-1  CACACTCGTAGCGCTC-1  CACACTCGTCTAGCCG-1  CACAGGCAGGCGTACA-1  CACAGGCAGTATCGAA-1  CACAGGCCACCGAATT-1  CACAGGCCAGGGTTAG-1  CACAGGCCATCCGCGA-1  CACAGGCTCTCTAGGA-1  CACAGTAAGCGTAGTG-1  CACAGTAAGGCGCTCT-1  CACAGTACACCTGGTG-1  CACAGTACATTATCTC-1  CACAGTAGTAACGCGA-1  CACAGTATCCTTTCGG-1  CACAGTATCGGCGCTA-1  CACATAGCACTGTGTA-1  CACATTTAGTCGTACT-1  CACATTTTCGGTCCGA-1  CACCACTAGCGCTCCA-1  CACCACTCACGCATCG-1  CACCACTCATGTCTCC-1  CACCAGGCAGGGTTAG-1  CACCAGGCATCTCCCA-1  CACCAGGTCTGAGGGA-1  CACCAGGTCTTCAACT-1  CACCTTGCATCCGTGG-1  CACCTTGGTAGCACGA-1  CACCTTGGTGGTACAG-1  CACCTTGGTTGAGTTC-1  CACTCCAAGATCCCAT-1  CACTCCAAGGCAATTA-1  CACTCCAAGTCGAGTG-1  CACTCCAGTCGGGTCT-1  CACTCCAGTCTAAACC-1  CACTCCATCTTCATGT-1  CAGAATCAGACTTGAA-1  CAGAATCAGTAAGTAC-1  CAGAATCCAGGAACGT-1  CAGAATCGTAGTAGTA-1  CAGAATCTCTTCTGGC-1  CAGAGAGAGAACTGTA-1  CAGAGAGCAGTTCCCT-1  CAGAGAGGTCCAGTAT-1  CAGAGAGGTTAGTGGG-1  CAGAGAGGTTGGACCC-1  CAGAGAGTCAGAGACG-1  CAGATCAAGGAATTAC-1  CAGATCATCATGCAAC-1  CAGCAGCCAATTGCTG-1  CAGCAGCGTGACGGTA-1  CAGCAGCGTGTGCGTC-1  CAGCAGCGTGTTTGTG-1  CAGCAGCTCTAAGCCA-1  CAGCATAAGCGTGTCC-1  CAGCATATCGTTACAG-1  CAGCCGAGTGACAAAT-1  CAGCCGAGTGAGGGTT-1  CAGCCGATCATTATCC-1  CAGCCGATCCTAAGTG-1  CAGCGACAGAATTCCC-1  CAGCGACAGCGATATA-1  CAGCGACGTTCAACCA-1  CAGCTAACAAACTGTC-1  CAGCTAACAGGTTTCA-1  CAGCTAAGTCCCTACT-1  CAGCTAATCAACGCTA-1  CAGCTGGCAGGCTGAA-1  CAGCTGGGTAAACCTC-1  CAGCTGGGTACTCGCG-1  CAGCTGGGTCGAGTTT-1  CAGCTGGTCAGCTCGG-1  CAGCTGGTCTCGGACG-1  CAGGTGCAGGCAGTCA-1  CAGGTGCAGTTATCGC-1  CAGGTGCGTAGCACGA-1  CAGGTGCGTTTGGCGC-1  CAGTAACAGGAATGGA-1  CAGTAACCAAATCCGT-1  CAGTAACCACATTTCT-1  CAGTCCTAGTAACCCT-1  CAGTCCTGTGTGCGTC-1  CAGTCCTGTGTGGCTC-1  CAGTCCTTCCATGCTC-1  CAGTCCTTCTGCGTAA-1  CATATGGAGTGAAGAG-1  CATATGGGTTCCGTCT-1  CATATGGTCAACTCTT-1  CATATGGTCTCCAGGG-1  CATATTCCAACACCTA-1  CATATTCCATCTATGG-1  CATATTCGTAAACGCG-1  CATATTCTCATCATTC-1  CATATTCTCGCAAGCC-1  CATATTCTCTAGAGTC-1  CATCAAGAGTCATCCA-1  CATCAAGCATTTCACT-1  CATCAAGGTAGCGTCC-1  CATCAAGTCGTTGCCT-1  CATCAGAAGTGGTCCC-1  CATCAGACAAGGTTCT-1  CATCAGAGTTTGCATG-1  CATCAGATCAACGGGA-1  CATCAGATCTATCCCG-1  CATCCACCATTTGCCC-1  CATCCACGTTCCTCCA-1  CATCCACTCGGCATCG-1  CATCGAAAGATCCCAT-1  CATCGAAAGGATATAC-1  CATCGAACACGGCGTT-1  CATCGAAGTGCGATAG-1  CATCGAATCAAGGTAA-1  CATCGGGCAGTAACGG-1  CATCGGGGTCTAAACC-1  CATCGGGGTGACGCCT-1  CATGACAAGACGACGT-1  CATGACAAGAGTACCG-1  CATGACACACGAGGTA-1  CATGACACAGGACCCT-1  CATGACAGTTCCCGAG-1  CATGACAGTTGGTTTG-1  CATGACATCATATCGG-1  CATGACATCTTTAGTC-1  CATGCCTCACTATCTT-1  CATGCCTCATGTCTCC-1  CATGCCTGTTCCACTC-1  CATGCCTTCTTATCTG-1  CATGGCGAGAAACCAT-1  CATGGCGGTATTAGCC-1  CATGGCGGTTCGGGCT-1  CATGGCGTCGAATGGG-1  CATTATCAGCGTGAAC-1  CATTATCCAACTTGAC-1  CATTATCGTAGAAAGG-1  CATTATCGTAGCGTGA-1  CATTATCGTTGGTGGA-1  CATTATCTCAACACCA-1  CATTATCTCGCCAAAT-1  CATTCGCAGATGAGAG-1  CATTCGCCAGCAGTTT-1  CATTCGCGTCAGCTAT-1  CATTCGCGTTGCGCAC-1  CCAATCCAGGTACTCT-1  CCAATCCCAGCATACT-1  CCAATCCTCTAACTCT-1  CCACCTAAGCTCCTCT-1  CCACCTAAGTAAGTAC-1  CCACCTAAGTACTTGC-1  CCACCTACAAACGTGG-1  CCACCTACAGCCTATA-1  CCACCTACAGGTGGAT-1  CCACCTAGTATAGGTA-1  CCACCTAGTGTGTGCC-1  CCACCTATCAACCATG-1  CCACCTATCTTGTCAT-1  CCACGGAAGATGTGGC-1  CCACGGACAAGCCCAC-1  CCACGGATCCCTTGTG-1  CCACTACAGACTAGAT-1  CCACTACGTTTGGGCC-1  CCACTACTCTCCTATA-1  CCAGCGAAGACACGAC-1  CCAGCGATCAGAAATG-1  CCATGTCGTCTCAACA-1  CCATGTCGTGATGATA-1  CCATGTCTCCATTCTA-1  CCATTCGAGCCTTGAT-1  CCATTCGAGGTGCACA-1  CCATTCGCAAAGTCAA-1  CCATTCGCAAGCCTAT-1  CCATTCGGTCGGATCC-1  CCCAATCTCATGTAGC-1  CCCAGTTTCTTGTCAT-1  CCCATACAGAACAATC-1  CCCATACAGCGATGAC-1  CCCATACAGCTGAAAT-1  CCCATACCATCGGGTC-1  CCCATACGTAAGGGAA-1  CCCATACGTACAAGTA-1  CCCATACTCCCTCTTT-1  CCCATACTCTCTAAGG-1  CCCTCCTAGCTTTGGT-1  CCCTCCTAGTACGATA-1  CCCTCCTAGTGCCAGA-1  CCCTCCTCACGGTGTC-1  CCCTCCTGTCTTGCGG-1  CCCTCCTGTGAGTATA-1  CCCTCCTTCGTAGGAG-1  CCCTCCTTCTTGCAAG-1  CCGGGATAGCTGCCCA-1  CCGGGATCACCAGGTC-1  CCGGGATGTAAACGCG-1  CCGGTAGAGACAGACC-1  CCGGTAGAGGAGTCTG-1  CCGGTAGAGTACACCT-1  CCGGTAGGTGTGCCTG-1  CCGGTAGTCCTTAATC-1  CCGTACTTCGTCTGCT-1  CCGTGGACACAGACAG-1  CCGTGGACATACGCCG-1  CCGTGGAGTCGCATCG-1  CCGTGGATCATAAAGG-1  CCGTGGATCTCTGCTG-1  CCGTTCAAGAACAACT-1  CCGTTCAAGAGGGATA-1  CCGTTCAAGGAATTAC-1  CCGTTCAAGGTGATAT-1  CCGTTCAAGTGGTAAT-1  CCGTTCAGTACCAGTT-1  CCGTTCAGTATAGGGC-1  CCGTTCAGTTAAAGTG-1  CCTAAAGAGAGGACGG-1  CCTAAAGAGCCATCGC-1  CCTAAAGAGGCCCGTT-1  CCTAAAGCAAGTCTAC-1  CCTAAAGGTGATGCCC-1  CCTAAAGTCAAACGGG-1  CCTACACAGAAGGTTT-1  CCTACACAGACTTTCG-1  CCTACACAGCCCGAAA-1  CCTACACCACCATGTA-1  CCTACACCAGATGGCA-1  CCTACACGTCTTGCGG-1  CCTACACTCAGTACGT-1  CCTACACTCCCGACTT-1  CCTACCAAGACACGAC-1  CCTACCAGTGTTGGGA-1  CCTACCAGTTCGTCTC-1  CCTACCATCATACGGT-1  CCTACCATCGTACCGG-1  CCTACCATCGTTACGA-1  CCTAGCTAGCGTTCCG-1  CCTAGCTAGCTAACAA-1  CCTAGCTCATCGACGC-1  CCTAGCTGTCCTCCAT-1  CCTAGCTGTTCACGGC-1  CCTAGCTTCAAACAAG-1  CCTAGCTTCCTGTAGA-1  CCTATTAAGTTATCGC-1  CCTATTATCCTACAGA-1  CCTCAGTAGGAGTTGC-1  CCTCAGTAGTAACCCT-1  CCTCAGTCAATAGAGT-1  CCTCAGTCACGCATCG-1  CCTCAGTCAGGTCTCG-1  CCTCAGTGTAGAGCTG-1  CCTCAGTTCAATCACG-1  CCTCAGTTCATAGCAC-1  CCTCAGTTCCGTAGGC-1  CCTCTGAAGATGGCGT-1  CCTCTGAAGCTGAACG-1  CCTCTGACAGGTCGTC-1  CCTCTGAGTGCAGACA-1  CCTCTGAGTGTCGCTG-1  CCTCTGAGTTCAACCA-1  CCTCTGATCCTTAATC-1  CCTCTGATCGGTGTCG-1  CCTTACGCAGACGCCT-1  CCTTACGCAGGGCATA-1  CCTTACGCATGCCACG-1  CCTTCCCAGCACAGGT-1  CCTTCCCCATGTAGTC-1  CCTTCCCGTACGAAAT-1  CCTTCCCGTCCAGTGC-1  CCTTCCCGTCCGAATT-1  CCTTCCCGTCGCGTGT-1  CCTTCCCGTGCTAGCC-1  CCTTCGAGTAGTGAAT-1  CCTTCGAGTCAATACC-1  CCTTTCTAGCAACGGT-1  CCTTTCTAGTCAAGGC-1  CCTTTCTTCAACGCTA-1  CCTTTCTTCACAAACC-1  CCTTTCTTCACAGTAC-1  CCTTTCTTCGTTGCCT-1  CGAACATAGCTACCTA-1  CGAACATAGGACAGCT-1  CGAACATCACAGTCGC-1  CGAACATCAGGAACGT-1  CGAACATCAGGGCATA-1  CGAACATCATTTGCCC-1  CGAATGTAGGTGCTAG-1  CGAATGTGTAGCCTCG-1  CGAATGTGTAGCGCAA-1  CGAATGTTCGACAGCC-1  CGAATGTTCTATCCTA-1  CGACCTTCACGCTTTC-1  CGACTTCAGTGAACGC-1  CGACTTCCACGACTCG-1  CGACTTCCAGGTCTCG-1  CGACTTCGTACCCAAT-1  CGACTTCGTCCGAATT-1  CGACTTCGTGGCGAAT-1  CGACTTCTCCAAACTG-1  CGAGAAGAGATGCGAC-1  CGAGAAGGTCTTCGTC-1  CGAGAAGTCACGATGT-1  CGAGAAGTCCCTTGTG-1  CGAGAAGTCGTGGACC-1  CGAGCACAGATCCTGT-1  CGAGCACCACATGACT-1  CGAGCACCACCGAAAG-1  CGAGCACGTGCTCTTC-1  CGAGCACTCATAAAGG-1  CGAGCCAAGGATGGTC-1  CGAGCCACAGCTTCGG-1  CGAGCCATCAGAGCTT-1  CGAGCCATCCCAAGAT-1  CGATCGGCAACTGCTA-1  CGATCGGGTAGCGCAA-1  CGATCGGTCTGCCAGG-1  CGATCGGTCTGTTGAG-1  CGATGGCAGCCACGTC-1  CGATGGCGTTTGTTGG-1  CGATGGCTCAGTTTGG-1  CGATGTAAGAGAGCTC-1  CGATGTAAGTGTACCT-1  CGATGTACAAGTTCTG-1  CGATGTAGTGGTGTAG-1  CGATGTAGTTCGCGAC-1  CGATGTATCAGAGGTG-1  CGATGTATCGTGTAGT-1  CGATGTATCTCGCATC-1  CGATTGACACTGCCAG-1  CGATTGACATCGATTG-1  CGATTGAGTCTTGCGG-1  CGCCAAGTCCTCGCAT-1  CGCGGTAAGCAGCGTA-1  CGCGGTAAGCGTGTCC-1  CGCGGTACACTTAACG-1  CGCGGTACATACTACG-1  CGCGGTACATCCTAGA-1  CGCGGTAGTTCTGGTA-1  CGCGGTATCGACAGCC-1  CGCGTTTCAATAGCAA-1  CGCGTTTCAGATCTGT-1  CGCGTTTCAGCTGGCT-1  CGCGTTTGTACTTCTT-1  CGCGTTTGTAGCCTCG-1  CGCGTTTGTCTAGAGG-1  CGCGTTTGTGGAAAGA-1  CGCGTTTTCCACGTTC-1  CGCTATCAGGCAAAGA-1  CGCTATCGTATGGTTC-1  CGCTATCGTTCAGACT-1  CGCTGGAAGGCCCGTT-1  CGCTGGAAGTGTCCCG-1  CGCTGGACAGACACTT-1  CGCTGGAGTACGCTGC-1  CGCTGGAGTCGCATAT-1  CGCTGGAGTTTGCATG-1  CGCTTCAAGCGATTCT-1  CGCTTCACAGGGATTG-1  CGGACACCACGGCTAC-1  CGGACACCACGTAAGG-1  CGGACACGTATAGGTA-1  CGGACACGTTCCACAA-1  CGGACACTCAAACAAG-1  CGGACACTCCGAACGC-1  CGGACGTCATTATCTC-1  CGGACGTGTATGAATG-1  CGGACGTTCAACCATG-1  CGGACGTTCGGAATCT-1  CGGACTGAGCTAGCCC-1  CGGACTGAGTGTACCT-1  CGGACTGCAATCCGAT-1  CGGACTGTCAATCACG-1  CGGACTGTCTACTATC-1  CGGAGCTAGCCGCCTA-1  CGGAGCTAGGCTCAGA-1  CGGAGCTCACTTACGA-1  CGGAGCTGTACCTACA-1  CGGAGCTGTCTCGTTC-1  CGGAGCTTCCACTCCA-1  CGGAGTCAGTGGACGT-1  CGGAGTCAGTTGTAGA-1  CGGAGTCCATGCCTAA-1  CGGAGTCGTAGCTAAA-1  CGGCTAGAGAATCTCC-1  CGGCTAGCAATAGCGG-1  CGGCTAGCACAGAGGT-1  CGGGTCATCAGGCGAA-1  CGGGTCATCGTGGTCG-1  CGGTTAAGTGATGCCC-1  CGTAGCGAGAGAACAG-1  CGTAGCGAGCGTAGTG-1  CGTAGCGAGTAGCGGT-1  CGTAGCGGTAACGTTC-1  CGTAGCGGTTGCGTTA-1  CGTAGCGTCGATGAGG-1  CGTAGGCAGCTCCTCT-1  CGTAGGCCATACAGCT-1  CGTAGGCGTGTTTGTG-1  CGTAGGCTCCACGACG-1  CGTAGGCTCCAGTAGT-1  CGTAGGCTCTTTACAC-1  CGTCACTGTAACGTTC-1  CGTCACTTCCGGCACA-1  CGTCAGGAGGTCATCT-1  CGTCAGGGTGCAGACA-1  CGTCAGGTCCTCCTAG-1  CGTCAGGTCGCGTTTC-1  CGTCCATCACCGCTAG-1  CGTCCATGTCGACTGC-1  CGTCTACCACGTCTCT-1  CGTCTACGTACAGCAG-1  CGTCTACGTTTAGCTG-1  CGTCTACTCTCTAGGA-1  CGTCTACTCTGTGCAA-1  CGTGAGCCACTTAACG-1  CGTGAGCGTCCGCTGA-1  CGTGAGCGTCGCATCG-1  CGTGTAAGTAATAGCA-1  CGTGTAAGTCGACTAT-1  CGTGTCTCATCACAAC-1  CGTGTCTCATTACGAC-1  CGTGTCTGTACCGGCT-1  CGTGTCTGTAGAGCTG-1  CGTTAGACAATCAGAA-1  CGTTAGACATTGGTAC-1  CGTTAGAGTAAGGATT-1  CGTTAGAGTTTAAGCC-1  CGTTAGATCATTATCC-1  CGTTAGATCTCTGCTG-1  CGTTCTGCAGACGCCT-1  CGTTCTGGTGTGGCTC-1  CGTTGGGAGATGCCAG-1  CGTTGGGAGGGTTTCT-1  CGTTGGGGTACCTACA-1  CGTTGGGGTCGTGGCT-1  CGTTGGGGTCTCCATC-1  CGTTGGGTCACCAGGC-1  CGTTGGGTCTCGAGTA-1  CTAACTTGTACCAGTT-1  CTAACTTTCATTATCC-1  CTAAGACAGAGTTGGC-1  CTAAGACAGATGTTAG-1  CTAAGACCAGGACGTA-1  CTAAGACTCAAACGGG-1  CTACACCCACTACAGT-1  CTACACCCAGAAGCAC-1  CTACACCCAGCGATCC-1  CTACACCCAGTCAGAG-1  CTACACCCAGTGACAG-1  CTACACCGTACGCACC-1  CTACACCGTCACTTCC-1  CTACACCGTCCTGCTT-1  CTACACCGTTAAAGTG-1  CTACATTAGACAATAC-1  CTACATTAGATGTCGG-1  CTACATTTCACGCATA-1  CTACATTTCAGTTTGG-1  CTACATTTCTCGAGTA-1  CTACCCAAGACCTAGG-1  CTACCCAAGACGCACA-1  CTACCCAAGAGTCTGG-1  CTACCCAAGGAGTCTG-1  CTACCCATCCTTCAAT-1  CTACCCATCGCGGATC-1  CTACGTCCAACTGCGC-1  CTACGTCGTAAGGGCT-1  CTACGTCGTTAAGGGC-1  CTAGAGTAGCACAGGT-1  CTAGAGTCATATACCG-1  CTAGAGTCATTACGAC-1  CTAGAGTGTCAAAGAT-1  CTAGCCTAGGACATTA-1  CTAGCCTAGGTAGCCA-1  CTAGCCTGTCCGAACC-1  CTAGCCTGTGATGCCC-1  CTAGCCTGTGGGTCAA-1  CTAGCCTTCCCAGGTG-1  CTAGCCTTCCTATGTT-1  CTAGTGAAGTATTGGA-1  CTAGTGACAAAGAATC-1  CTAGTGACAGGGTTAG-1  CTCACACAGCCGCCTA-1  CTCACACAGTGTGGCA-1  CTCACACCATACTCTT-1  CTCACACGTTCACGGC-1  CTCAGAAAGCCGCCTA-1  CTCAGAAAGGCAGGTT-1  CTCAGAAAGTGCGTGA-1  CTCAGAAAGTGCTGCC-1  CTCAGAAAGTGGTAAT-1  CTCAGAATCCCATTAT-1  CTCATTAAGGTACTCT-1  CTCATTACAACCGCCA-1  CTCATTACACAGCGTC-1  CTCATTACAGGGTACA-1  CTCATTAGTGATGATA-1  CTCCTAGAGATCTGAA-1  CTCCTAGAGGGAAACA-1  CTCCTAGAGTGTGGCA-1  CTCCTAGCAAATTGCC-1  CTCCTAGGTGCTAGCC-1  CTCGAAAAGAGGGATA-1  CTCGAAAAGCTTCGCG-1  CTCGAAATCCTGCCAT-1  CTCGAGGCAAGCCGTC-1  CTCGAGGTCAGGCGAA-1  CTCGAGGTCAGTTAGC-1  CTCGAGGTCTGCCAGG-1  CTCGGAGGTAATTGGA-1  CTCGGAGTCACCCTCA-1  CTCGGAGTCGAATGGG-1  CTCGGGAAGCCGCCTA-1  CTCGGGAAGGATGGAA-1  CTCGGGAAGTGGGATC-1  CTCGGGACATGGTCAT-1  CTCGGGATCCGAATGT-1  CTCGTACCATTATCTC-1  CTCGTACGTTTGACTG-1  CTCGTACTCGGTCTAA-1  CTCGTCAAGCAGACTG-1  CTCGTCACAAGTTAAG-1  CTCGTCAGTACAGTTC-1  CTCGTCAGTTAGGGTG-1  CTCGTCATCACTGGGC-1  CTCGTCATCTGTCTCG-1  CTCTAATGTAGAAAGG-1  CTCTAATGTCGACTGC-1  CTCTAATGTCGATTGT-1  CTCTAATTCGAATCCA-1  CTCTAATTCTGATACG-1  CTCTACGAGAGACTTA-1  CTCTACGAGCGTAGTG-1  CTCTACGGTAGGGTAC-1  CTCTACGGTTCGTCTC-1  CTCTACGTCGGACAAG-1  CTCTACGTCGGCTTGG-1  CTCTACGTCTGGAGCC-1  CTCTGGTGTTCGGCAC-1  CTCTGGTTCACCGTAA-1  CTGAAACAGGACTGGT-1  CTGAAACTCAGTCAGT-1  CTGAAGTAGTTAGGTA-1  CTGAAGTCACTTCGAA-1  CTGAAGTGTCGACTAT-1  CTGAAGTTCGCTGATA-1  CTGATAGGTTCACCTC-1  CTGATAGTCAAAGTAG-1  CTGATAGTCCCAACGG-1  CTGATCCAGAAGGCCT-1  CTGATCCCAATCGGTT-1  CTGATCCCACAGGTTT-1  CTGATCCGTCTACCTC-1  CTGATCCGTCTCCATC-1  CTGATCCTCACATACG-1  CTGCCTAAGCCAGTAG-1  CTGCCTAAGTGCGTGA-1  CTGCCTAAGTTTCCTT-1  CTGCCTACATTACCTT-1  CTGCCTAGTTGGGACA-1  CTGCCTATCCCTAACC-1  CTGCCTATCTGCAAGT-1  CTGCGGACAGTACACT-1  CTGCGGACATTACCTT-1  CTGCGGAGTTCGTCTC-1  CTGCGGATCTTTACGT-1  CTGCTGTCAAGAAGAG-1  CTGCTGTCAAGCCATT-1  CTGCTGTGTCATGCAT-1  CTGCTGTGTTAAAGTG-1  CTGCTGTGTTCCCTTG-1  CTGGTCTCACCATCCT-1  CTGGTCTGTTAAAGAC-1  CTGTGCTGTCACCCAG-1  CTGTGCTGTTTGTGTG-1  CTGTTTACATGTCTCC-1  CTGTTTAGTAGGGACT-1  CTGTTTAGTGGTCCGT-1  CTGTTTAGTTGAACTC-1  CTGTTTATCGATAGAA-1  CTTAACTCACTTGGAT-1  CTTAACTTCTAACTTC-1  CTTACCGCAGGAACGT-1  CTTACCGCATGGATGG-1  CTTACCGGTCAGAATA-1  CTTACCGGTTCAACCA-1  CTTAGGAAGCTAAGAT-1  CTTAGGAAGTAATCCC-1  CTTAGGACAAATCCGT-1  CTTAGGATCCCAGGTG-1  CTTCTCTGTCGCGTGT-1  CTTGGCTAGCTGAAAT-1  CTTGGCTCACGGCGTT-1  CTTGGCTCAGTGGAGT-1  CTTTGCGAGATCGATA-1  CTTTGCGAGTGGCACA-1  CTTTGCGTCTTGTCAT-1  GAAACTCAGGTGCACA-1  GAAACTCCACCCAGTG-1  GAAACTCGTACATCCA-1  GAAATGAAGAATTGTG-1  GAAATGAAGTGAAGAG-1  GAAATGACATCCGTGG-1  GAAATGAGTAATCACC-1  GAAATGATCAAACCAC-1  GAACATCGTCTGATCA-1  GAACCTAGTCTAGTCA-1  GAACCTAGTTTGTTTC-1  GAACGGAAGGCAGGTT-1  GAACGGAAGGTGCTAG-1  GAACGGACAAGCCGCT-1  GAACGGACACCCATGG-1  GAACGGAGTGGCAAAC-1  GAACGGAGTTCGAATC-1  GAAGCAGTCTCGCATC-1  GAATAAGAGAGCCCAA-1  GAATAAGAGCCTTGAT-1  GAATAAGCAGACGCAA-1  GAATAAGGTACATGTC-1  GAATGAAAGAACTCGG-1  GAATGAAAGTTTCCTT-1  GAATGAACAAGTTCTG-1  GACACGCAGACTGTAA-1  GACACGCAGAGTGACC-1  GACACGCAGTAGCGGT-1  GACACGCCAATGTAAG-1  GACACGCCACAGCGTC-1  GACACGCGTCATATGC-1  GACACGCTCAACACGT-1  GACAGAGAGCGCCTTG-1  GACAGAGAGGGAACGG-1  GACAGAGCAAAGTCAA-1  GACAGAGCACATGTGT-1  GACAGAGCATGCTGGC-1  GACAGAGGTAATCGTC-1  GACAGAGGTCTCGTTC-1  GACAGAGTCAACGCTA-1  GACAGAGTCACAGTAC-1  GACAGAGTCTCAAGTG-1  GACCAATAGCTACCGC-1  GACCAATAGTGGTAGC-1  GACCAATCACAGACAG-1  GACCAATTCCCACTTG-1  GACCAATTCTGTGCAA-1  GACCTGGGTATAGGTA-1  GACCTGGGTCCTAGCG-1  GACGCGTAGGACGAAA-1  GACGCGTCAGATAATG-1  GACGCGTCAGCTGGCT-1  GACGCGTGTGGAAAGA-1  GACGCGTTCTATCCCG-1  GACGGCTAGCGTTCCG-1  GACGGCTCACGAAACG-1  GACGTGCAGCGCCTTG-1  GACGTGCAGGGAGTAA-1  GACGTGCTCTAACTGG-1  GACGTTAGTCTTCTCG-1  GACGTTATCGGCTACG-1  GACTAACAGAGTCTGG-1  GACTAACAGTCGTTTG-1  GACTAACCATCGGACC-1  GACTAACTCACAGTAC-1  GACTAACTCTTAGAGC-1  GACTACAGTAGCGATG-1  GACTACAGTTAAGTAG-1  GACTGCGAGTACTTGC-1  GACTGCGAGTGAACAT-1  GACTGCGCACATCCGG-1  GACTGCGGTACCTACA-1  GACTGCGGTGCCTGGT-1  GACTGCGTCTTTCCTC-1  GAGCAGAGTCCGAGTC-1  GAGCAGAGTGAGCGAT-1  GAGGTGAAGAACAATC-1  GAGGTGACAGGTCGTC-1  GAGTCCGAGTAGCGGT-1  GAGTCCGAGTGTCTCA-1  GAGTCCGGTCCCGACA-1  GAGTCCGGTGAAAGAG-1  GAGTCCGTCTATCCTA-1  GATCAGTCAACTGCTA-1  GATCAGTGTCAGAATA-1  GATCAGTTCCGCATCT-1  GATCAGTTCTATCGCC-1  GATCGATCAAGCGATG-1  GATCGATGTGCCTGTG-1  GATCGATTCACCACCT-1  GATCGATTCTGTCTCG-1  GATCGCGAGCCAGAAC-1  GATCGCGTCCGCAGTG-1  GATCGTAAGGTCATCT-1  GATCGTAAGTGAATTG-1  GATCGTAGTCCAGTTA-1  GATCGTAGTCCTAGCG-1  GATCGTAGTGTGGCTC-1  GATCTAGAGAGCCTAG-1  GATCTAGAGTAAGTAC-1  GATCTAGCAAAGGTGC-1  GATCTAGGTTCAGGCC-1  GATCTAGGTTGTGGAG-1  GATCTAGTCTTCATGT-1  GATGAAACAAAGTCAA-1  GATGAAACATGGAATA-1  GATGAAAGTGAGTATA-1  GATGAAAGTGCCTTGG-1  GATGAAATCCACGAAT-1  GATGAAATCTGTACGA-1  GATGAGGAGATGCGAC-1  GATGAGGAGGGTTCCC-1  GATGAGGCAGTTTACG-1  GATGAGGTCGCAAGCC-1  GATGAGGTCTGTACGA-1  GATGCTAAGTCCAGGA-1  GATGCTAGTGTTCTTT-1  GATTCAGAGCAGGTCA-1  GATTCAGCAATGAATG-1  GATTCAGGTAAACACA-1  GATTCAGGTAACGCGA-1  GATTCAGGTAGCACGA-1  GATTCAGGTGAGTATA-1  GCAAACTCACCCATGG-1  GCAAACTCAGTTCCCT-1  GCAATCAAGTACACCT-1  GCAATCAAGTACGCGA-1  GCAATCACAAGCCGTC-1  GCAATCACAGTTAACC-1  GCAATCAGTACCAGTT-1  GCAATCAGTGAGGGAG-1  GCAATCAGTGTCGCTG-1  GCAATCAGTTCAGGCC-1  GCACATAAGGCTAGCA-1  GCACATACAGGTCTCG-1  GCACATAGTAGCTAAA-1  GCACATATCCTCAACC-1  GCACATATCTTTAGGG-1  GCACTCTCATGTCCTC-1  GCACTCTTCCAGATCA-1  GCACTCTTCCCTTGCA-1  GCAGCCAAGACGCTTT-1  GCAGCCAAGTCCAGGA-1  GCAGCCACAAGGCTCC-1  GCAGCCAGTAGGGTAC-1  GCAGCCATCTGCAAGT-1  GCAGTTAAGAGCTGCA-1  GCAGTTACAAGCGATG-1  GCAGTTAGTAGCGCTC-1  GCAGTTATCGTACGGC-1  GCATACAAGATTACCC-1  GCATACAAGCTGGAAC-1  GCATACAAGTATTGGA-1  GCATACACAGACGTAG-1  GCATACAGTCTGGAGA-1  GCATACATCCACGCAG-1  GCATGATAGAATGTGT-1  GCATGATAGGCATTGG-1  GCATGATAGTTCGATC-1  GCATGATTCAACTCTT-1  GCATGCGAGCGATTCT-1  GCATGCGCACGCGAAA-1  GCATGCGGTCAAAGAT-1  GCATGCGTCGAGAGCA-1  GCATGTAAGTCGCCGT-1  GCATGTACAGATGGCA-1  GCATGTAGTGACGCCT-1  GCATGTAGTGTTCTTT-1  GCATGTATCATATCGG-1  GCATGTATCTTTCCTC-1  GCCAAATAGAATTGTG-1  GCCAAATCAACACCCG-1  GCCAAATCAACACCTA-1  GCCAAATCACGAGGTA-1  GCCAAATGTCGCTTCT-1  GCCAAATGTTCCTCCA-1  GCCAAATTCCGCTGTT-1  GCCAAATTCTTTACAC-1  GCCTCTAAGTTAAGTG-1  GCCTCTACAGCTGTTA-1  GCCTCTAGTCCGTTAA-1  GCCTCTAGTTCGCGAC-1  GCCTCTATCAATCTCT-1  GCGACCACACCATGTA-1  GCGACCACAGATCCAT-1  GCGACCAGTAGCTCCG-1  GCGACCATCAGCAACT-1  GCGACCATCGGTGTTA-1  GCGACCATCTAGAGTC-1  GCGACCATCTCGCATC-1  GCGAGAAAGGTGATTA-1  GCGAGAACAATGACCT-1  GCGAGAACACTGCCAG-1  GCGAGAAGTAATCGTC-1  GCGAGAAGTACGAAAT-1  GCGAGAAGTCAGAGGT-1  GCGAGAAGTTTGCATG-1  GCGAGAATCTCTGCTG-1  GCGCAACAGGCTATCT-1  GCGCAACAGTGAAGAG-1  GCGCAACCAACAACCT-1  GCGCAACCACTTAAGC-1  GCGCAACTCCTGCCAT-1  GCGCAACTCTCTAGGA-1  GCGCAGTCAGTAAGAT-1  GCGCAGTTCATGCAAC-1  GCGCAGTTCTTCTGGC-1  GCGCCAAGTAAACCTC-1  GCGCCAATCAGGCAAG-1  GCGCGATTCATTTGGG-1  GCGGGTTCAAGTCTGT-1  GCGGGTTTCACCTTAT-1  GCGGGTTTCCAAAGTC-1  GCTCCTAAGACTAAGT-1  GCTCCTAGTACATCCA-1  GCTCCTAGTTATCCGA-1  GCTCCTATCAGCTTAG-1  GCTCCTATCGGCCGAT-1  GCTCCTATCTAACTCT-1  GCTCTGTAGGCGATAC-1  GCTCTGTCAGCTCGCA-1  GCTCTGTGTCTCTTAT-1  GCTCTGTTCACAGGCC-1  GCTGCAGGTAACGTTC-1  GCTGCAGGTCCATCCT-1  GCTGCAGGTTTGTTGG-1  GCTGCAGTCACGATGT-1  GCTGCAGTCTCGCTTG-1  GCTGCAGTCTGATACG-1  GCTGCGAAGCTGTCTA-1  GCTGCGACACCGAAAG-1  GCTGCGACATGTTGAC-1  GCTGCTTAGGACAGCT-1  GCTGCTTAGGGAGTAA-1  GCTGCTTAGGTTCCTA-1  GCTGCTTCATCGGTTA-1  GCTGCTTTCATGTAGC-1  GCTGCTTTCCGTTGCT-1  GCTGGGTAGATCCCGC-1  GCTGGGTAGCCAACAG-1  GCTGGGTAGTAGGTGC-1  GCTGGGTCAGTGGAGT-1  GCTGGGTGTCACAAGG-1  GCTGGGTGTGAGTATA-1  GCTGGGTGTTGTTTGG-1  GCTGGGTTCAGTCAGT-1  GCTGGGTTCAGTGTTG-1  GCTGGGTTCCGAAGAG-1  GCTGGGTTCGCGATCG-1  GCTTCCAGTTAAGACA-1  GCTTCCATCACGAAGG-1  GCTTCCATCCTGCAGG-1  GCTTCCATCTAACCGA-1  GCTTGAAAGTTAACGA-1  GCTTGAATCGACAGCC-1  GGAAAGCAGGTGCACA-1  GGAACTTCACGAAAGC-1  GGAACTTCAGACGCTC-1  GGAACTTCATCGGACC-1  GGAACTTGTAGCTGCC-1  GGAACTTGTTAGTGGG-1  GGAACTTTCAAGATCC-1  GGAACTTTCACTATTC-1  GGAACTTTCTGAAAGA-1  GGAATAAAGGGCATGT-1  GGAATAAGTCACAAGG-1  GGAATAAGTCCTCTTG-1  GGAATAATCAAGAAGT-1  GGACAAGAGTGCTGCC-1  GGACAAGGTCTCAACA-1  GGACAAGGTGAGTGAC-1  GGACAAGGTTGGTAAA-1  GGACAGACAAACGCGA-1  GGACAGACACGGATAG-1  GGACATTAGAATTGTG-1  GGACATTAGAGAGCTC-1  GGACATTCACCCATGG-1  GGACATTCAGGGTACA-1  GGACATTGTCAAAGCG-1  GGACATTGTGAGGCTA-1  GGACATTGTTCTGAAC-1  GGACATTTCAGTTTGG-1  GGACGTCAGATCGATA-1  GGACGTCGTAAACGCG-1  GGACGTCGTCTGCAAT-1  GGACGTCGTCTTCGTC-1  GGACGTCTCCTCCTAG-1  GGAGCAAAGAAGGTTT-1  GGAGCAAAGCTAAGAT-1  GGAGCAAAGTGGTAAT-1  GGAGCAACATAGAAAC-1  GGAGCAACATCAGTAC-1  GGAGCAAGTCGCTTCT-1  GGAGCAATCACAGTAC-1  GGAGCAATCGCGGATC-1  GGATGTTTCACTGGGC-1  GGATGTTTCTCTGCTG-1  GGATTACAGTCTCGGC-1  GGATTACAGTGTGAAT-1  GGATTACGTGACGGTA-1  GGCAATTAGGGTCGAT-1  GGCAATTTCCGTCAAA-1  GGCAATTTCGTTACGA-1  GGCCGATAGATATGCA-1  GGCCGATAGCCTATGT-1  GGCCGATCAATCTACG-1  GGCCGATTCTCGGACG-1  GGCGACTAGATCCCAT-1  GGCGACTGTCTGGTCG-1  GGCGTGTCATATACGC-1  GGCGTGTGTTAGATGA-1  GGCTCGAAGTTAAGTG-1  GGCTCGACATGCCCGA-1  GGCTGGTAGACGACGT-1  GGCTGGTCAAGTACCT-1  GGCTGGTCAGTCGTGC-1  GGCTGGTCATGATCCA-1  GGCTGGTTCCTTTACA-1  GGCTGGTTCTACGAGT-1  GGGAATGAGAGCAATT-1  GGGAATGCAAAGGCGT-1  GGGAATGCACTTAACG-1  GGGAATGGTGATGATA-1  GGGACCTAGGCGACAT-1  GGGACCTGTATGCTTG-1  GGGAGATAGCTAGGCA-1  GGGAGATAGGTGTTAA-1  GGGAGATCATATACGC-1  GGGAGATGTTACGCGC-1  GGGATGAAGTGGTCCC-1  GGGATGACAGCATGAG-1  GGGATGAGTATGGTTC-1  GGGATGAGTATTCTCT-1  GGGCACTAGTCCGGTC-1  GGGCACTCAAAGCAAT-1  GGGCACTTCCTCATTA-1  GGGCATCAGCGGATCA-1  GGGCATCAGTCGCCGT-1  GGGCATCCAACGATCT-1  GGGCATCCAGCCTATA-1  GGGTCTGAGACTTTCG-1  GGGTCTGAGATAGCAT-1  GGGTCTGAGCAATCTC-1  GGGTCTGCATTCTCAT-1  GGGTCTGTCCCTTGCA-1  GGGTTGCAGGCATGGT-1  GGGTTGCGTGCTGTAT-1  GGGTTGCTCAAACCAC-1  GGGTTGCTCGGATGGA-1  GGGTTGCTCGGCCGAT-1  GGGTTGCTCTGACCTC-1  GGTATTGAGACTCGGA-1  GGTATTGGTCCAACTA-1  GGTATTGTCACTGGGC-1  GGTATTGTCATAGCAC-1  GGTGCGTAGTCTTGCA-1  GGTGCGTCATACGCCG-1  GGTGCGTTCAGCTGGC-1  GGTGCGTTCTAACTGG-1  GGTGTTACAAACCTAC-1  GGTGTTACACCCATGG-1  GGTGTTATCTAACCGA-1  GTAACGTAGTGGACGT-1  GTAACGTCACAGATTC-1  GTAACGTGTCAGTGGA-1  GTAACTGAGATGTAAC-1  GTAACTGAGGAACTGC-1  GTAACTGCACTGTCGG-1  GTAACTGTCACCCGAG-1  GTAACTGTCAGCATGT-1  GTAACTGTCGTATCAG-1  GTACGTACAGACACTT-1  GTACGTACAGACGCAA-1  GTACGTACAGCCACCA-1  GTACGTAGTCATGCAT-1  GTACGTAGTCTTGATG-1  GTACGTAGTTGAGGTG-1  GTACGTATCTCGTATT-1  GTACGTATCTCTTGAT-1  GTACGTATCTTCGGTC-1  GTACTCCAGATATGGT-1  GTACTCCCAACTGCTA-1  GTACTCCCATACGCCG-1  GTACTCCGTATATCCG-1  GTACTCCGTCTAGTGT-1  GTACTCCGTGACCAAG-1  GTACTCCTCCCTCAGT-1  GTACTCCTCGGCGCTA-1  GTACTTTTCCAGGGCT-1  GTACTTTTCTACTCAT-1  GTAGGCCAGCCAGAAC-1  GTAGGCCAGTGTACTC-1  GTAGGCCGTGAACCTT-1  GTAGGCCTCCAAACTG-1  GTAGGCCTCGGGAGTA-1  GTAGTCAAGCGTAATA-1  GTAGTCACAAGTTCTG-1  GTAGTCACACCGAAAG-1  GTAGTCAGTGACGGTA-1  GTAGTCATCGGATGGA-1  GTATCTTCAACTGCTA-1  GTATCTTCAGACAAAT-1  GTATCTTTCTGGCGAC-1  GTATTCTCAGTTAACC-1  GTATTCTGTAAGTAGT-1  GTATTCTTCATAACCG-1  GTATTCTTCATCTGTT-1  GTCAAGTAGAGTACCG-1  GTCAAGTAGGATATAC-1  GTCAAGTAGGCCCGTT-1  GTCAAGTCATATACGC-1  GTCAAGTGTACCGCTG-1  GTCAAGTGTCGAACAG-1  GTCAAGTTCCAAATGC-1  GTCACAACACGCATCG-1  GTCACAAGTTAAGACA-1  GTCACAATCAGCCTAA-1  GTCACAATCCCTGACT-1  GTCACAATCGTACGGC-1  GTCACGGAGATAGGAG-1  GTCACGGCAAATTGCC-1  GTCACGGCAAGGTTTC-1  GTCACGGCATCACGTA-1  GTCACGGGTCTACCTC-1  GTCACGGTCACTATTC-1  GTCACGGTCTTTAGTC-1  GTCATTTAGTTCGCGC-1  GTCATTTCAATGGAAT-1  GTCCTCAAGGCTCAGA-1  GTCCTCACAAGACGTG-1  GTCCTCAGTAAATGAC-1  GTCCTCAGTTCGGGCT-1  GTCCTCATCAGCAACT-1  GTCGGGTAGGGAGTAA-1  GTCGGGTAGGGTTTCT-1  GTCGGGTCAAGTAGTA-1  GTCGGGTCAATAGCGG-1  GTCGGGTTCAGTGTTG-1  GTCGGGTTCATAGCAC-1  GTCGTAAAGATAGCAT-1  GTCGTAAAGGAGTAGA-1  GTCGTAATCACGGTTA-1  GTCGTAATCTTAGCCC-1  GTCTCGTAGAAGGACA-1  GTCTCGTAGACAAAGG-1  GTCTCGTGTTCTGAAC-1  GTCTCGTTCTTGAGAC-1  GTCTTCGAGTCAATAG-1  GTCTTCGGTACTCAAC-1  GTCTTCGTCCCGGATG-1  GTCTTCGTCCGATATG-1  GTCTTCGTCTTTACGT-1  GTGAAGGAGTTGAGAT-1  GTGAAGGCACATCTTT-1  GTGAAGGGTGCGCTTG-1  GTGAAGGGTTCGTCTC-1  GTGCAGCAGACAAAGG-1  GTGCAGCAGGCATGTG-1  GTGCAGCAGGGCTCTC-1  GTGCAGCAGTATGACA-1  GTGCAGCCATAGGATA-1  GTGCAGCCATCACGTA-1  GTGCAGCTCACCGGGT-1  GTGCAGCTCTTGCATT-1  GTGCATAAGTACGCCC-1  GTGCATAGTCGTTGTA-1  GTGCATAGTTCCCTTG-1  GTGCATAGTTCTGAAC-1  GTGCGGTAGATGTGTA-1  GTGCGGTAGGTGCTTT-1  GTGCGGTCAAGCCATT-1  GTGCGGTGTTTGCATG-1  GTGCTTCAGAAGGTGA-1  GTGCTTCGTAGAGTGC-1  GTGCTTCTCAGTTCGA-1  GTGGGTCAGACAATAC-1  GTGGGTCAGCCAGTTT-1  GTGGGTCAGCGCCTTG-1  GTGGGTCGTAGGGTAC-1  GTGGGTCGTCTAGCGC-1  GTGTGCGAGCGATATA-1  GTGTGCGGTCGCCATG-1  GTGTTAGCAAATTGCC-1  GTGTTAGCACTGAAGG-1  GTGTTAGCAGGAATCG-1  GTGTTAGGTAGCGTAG-1  GTGTTAGTCACATAGC-1  GTGTTAGTCATCACCC-1  GTTAAGCCAGGCGATA-1  GTTACAGCAATACGCT-1  GTTACAGCATCGGGTC-1  GTTACAGGTGATAAGT-1  GTTACAGTCTCATTCA-1  GTTACAGTCTGACCTC-1  GTTACAGTCTGGTTCC-1  GTTCGGGTCAACGGCC-1  GTTCGGGTCCCGACTT-1  GTTCTCGAGCCCAGCT-1  GTTCTCGAGCGCCTCA-1  GTTCTCGAGTGGGTTG-1  GTTCTCGCAGGGATTG-1  GTTCTCGGTAGGGACT-1  GTTCTCGTCATGGTCA-1  GTTTCTAAGCGTTTAC-1  GTTTCTAAGTCTCGGC-1  GTTTCTAGTCGCGGTT-1  GTTTCTAGTTGTTTGG-1  GTTTCTATCCACGAAT-1  GTTTCTATCCATGAAC-1  TAAACCGAGATCTGCT-1  TAAACCGGTCTAGGTT-1  TAAACCGGTCTAGTCA-1  TAAACCGGTGCACGAA-1  TAAACCGTCCTGTAGA-1  TAAGAGAAGATCCGAG-1  TAAGAGAAGGAGCGTT-1  TAAGAGAAGGCTCATT-1  TAAGAGACAAGGACAC-1  TAAGCGTAGGACCACA-1  TAAGCGTCACGAAAGC-1  TAAGCGTCACGCGAAA-1  TAAGCGTGTACATCCA-1  TAAGCGTGTTGTACAC-1  TAAGCGTTCGCCTGTT-1  TAAGCGTTCGTGGACC-1  TAAGTGCGTAACGTTC-1  TAAGTGCGTAGTAGTA-1  TAAGTGCGTCTTCGTC-1  TAAGTGCTCGCATGGC-1  TACACGACACCCAGTG-1  TACACGACAGCAGTTT-1  TACACGACATCGTCGG-1  TACACGAGTTCCGTCT-1  TACACGATCTTTACAC-1  TACAGTGCACGCCAGT-1  TACAGTGGTGCACTTA-1  TACAGTGGTTAAGAAC-1  TACAGTGTCACTATTC-1  TACCTATAGTACGCGA-1  TACCTATGTAATAGCA-1  TACCTATGTCTCCACT-1  TACCTATTCGACGGAA-1  TACCTTACACCAGGTC-1  TACCTTAGTGACGGTA-1  TACGGATAGACTAGGC-1  TACGGATCACGTCAGC-1  TACGGATGTCCGAATT-1  TACGGATGTGACTACT-1  TACGGATGTTCCTCCA-1  TACGGATTCAACGAAA-1  TACGGGCTCCCAAGAT-1  TACGGTAAGACTTGAA-1  TACGGTAAGATGCGAC-1  TACGGTAAGCCTTGAT-1  TACGGTACAAGCTGTT-1  TACGGTACACGCCAGT-1  TACGGTACACGGACAA-1  TACTCATGTAGCTTGT-1  TACTCATGTGGCGAAT-1  TACTCATGTGTGAAAT-1  TACTCATGTTGCGCAC-1  TACTCGCAGTGCTGCC-1  TACTTACAGAGTCTGG-1  TACTTACCAGTGGAGT-1  TACTTACCATCTATGG-1  TACTTACTCTACTCAT-1  TACTTGTAGAAGGGTA-1  TACTTGTGTACTCGCG-1  TACTTGTGTCAGCTAT-1  TACTTGTGTGCAGTAG-1  TACTTGTGTGCTGTAT-1  TACTTGTGTGTTTGTG-1  TACTTGTTCAGGCGAA-1  TACTTGTTCCAAACAC-1  TACTTGTTCCGCAGTG-1  TACTTGTTCCTTTACA-1  TAGACCAAGCCCAGCT-1  TAGACCACAAACCCAT-1  TAGACCACAGTAACGG-1  TAGACCACATTGGCGC-1  TAGACCAGTCATATGC-1  TAGACCATCACTTATC-1  TAGACCATCCCAACGG-1  TAGAGCTAGCGTTCCG-1  TAGAGCTAGTTACCCA-1  TAGAGCTCAAAGCAAT-1  TAGAGCTCATGACATC-1  TAGAGCTGTCTAGTGT-1  TAGAGCTTCTCCTATA-1  TAGCCGGCAACACCTA-1  TAGCCGGGTTATTCTC-1  TAGCCGGTCTGCCAGG-1  TAGGCATCAGC
POA10x_counts.csv
POA10x

data_processing:
gene expression counts matrix in csv format containing analyed POA E14.5 WT progenitor and early postmitotic interneurons  - samples in columns annotated by their barcodes, genes in rows with gene symbol notation
Cell Ranger software was used for mapping reads to the mm10 Mus Musculus genome.
Quality Control beyond Cell Ranger output: we discarded cells epressing less than 1000 genes or more than 15% mitochondrial reads. Non-interneuron populations were excluded by clustering.
mm10 v3.0.0.

source_name: embryonic brain POA

extract_protocol:
E14.5 C57BL/6 WT brains were extracted in ice-cold HBSS and sectioned. Preoptic Area microdissected tissue was then incubated in 0.05% Trypsin at 37°C for 5 minutes. After digestion, FBS was added and cells were mechanically dissociated via pipetting and centrifuged 5 minutes at 300g. Cell pellet was then suspended in 1ml HBSS and filtered using a 70um strainer.
Cell suspension was loaded into a 10X Chromium controller and processed with the Single Cell 3' v2 reagent kit. Single cel libraries were then sequenced using a HiSeq 4000 instrument.

value: embryonic brain POA

tag: developmental stage

value: interneuron precursor

tag: genotype

value: WT


title: S8_1

city: los angeles

first: Guoping

last: Fan

state: ca

address: 695 Charles E Young Drive South

department: Human Genetics

institute: university of california, los angeles

data_processing:
clean data were mapped to mouse genome mm10 by hisat2
reads count per gene is caculated by featurecount
Assembly: mm10
Supplementary files format and content: gene annotation with reads count

source_name: Cortex

extract_protocol:
Surgically resected cortical tissue was homogenized using a glass dounce homogenizer in 2 ml of ice-cold Nuclei EZ lysis buffer (#EZ PREP NUC-101, Sigma) and was incubated on ice for 5 min. Nuclei were centrifuged at 500 × g for 5 min at 4 °C, washed with 4 ml ice-cold Nuclei EZ lysis buffer and, incubated on ice for 5 min. Nuclei were centrifuged at 500 × g for 5 min at 4 °C. After centrifugation, the nuclei were resuspended in 1 ml of nuclei suspension buffer (NSB) consisting of 1XPBS, 1%BSA (#AM2618, Thermo Fisher Scientific) and 0.2U/ul RNAse inhibitor (#AM2694, Thermo Fisher Scientific) and were filtered through a 40-μm Flowmi Cell Strainer (#H13680-0040, Bel-Art). Nuclei concentration was determined using 0.4% Trypan Blue (#15250061, Thermo Fisher Scientific). Final concentration of 1000 nuclei/μl was adjusted with NSB.
Droplet-based snRNA-seq libraries were prepared using the Chromium Single Cell 3’ v2 according to the manufacturer’s protocol.

value: Cortex

value: C9orf72+/-;C9-BAC with AAV-Smcr8