title: Met5109T

city: Los Angeles

first: Lu

address: 10833 Le Conte Avenue

institute: University of California, LA

description: 10x Genomics single cell barcoded mRNA from CD45+ fraction, Sample #10

data_processing:
The reads are first mapped to GRCh38 using cellranger software from 10x Genomics
Single-cell, gene-level raw read counts are read from cellranger output and analyzed using Seurat 3.2.2
The Seurat R object from the Seurat object after IntegrateData and ScaleData step across 15 samples are provided as processed data file
Subsequent clustering and pseudotime trajectory analyses are descibed in the full manuscript
Genome_build: GRCh38
Supplementary_files_format_and_content: BrMOnly.scRNAseq_ObList.rds (RDS file)
MetsOnly.scRNAseq.Dec18.2020.normData.txt (normalized count)
BrMOnly.scRNAseq.SuffixSampleMap.xlsx (sample map of the colume name suffix in the normalized count table)

source_name: BrM

extract_protocol:
Dissociated CD45+ single cells were processed using Chromium Single Cell GEM, Library & Gel Bead Kit through the Chromium controller from 10x Genomics.
The resulting single cell barcoded mRNA were reverse transcribed and cleaned as per 10x Genomic's protocol.
10x Genomics single cell RNA-Seq

value: metastatic brain tumors

value: no prior immune checkpoint blockade therapy

tag: sequencing method

value: 3' sequencing

tag: gel bead version

value: dissociated CD45+ single cells


title: DP16_Noninfected, rep 4

status: Public on Jul 06 2023

type: SRA

city: Aurora

first: Andrew

last: Goodspeed

state: CO

address: 12801 E 17th Ave, Mail Stop 8303

country: USA

institute: University of Colorado Anschutz Medical Campus

library_source: transcriptomic

data_processing:
Illumina adapters were removed using BBDuk and reads <50bp after trimming were discarded
Reads were aligned and quantified using STAR (2.6.0a) to the Ensembl mouse transcriptome (GRCm38.p6, release 96)
Lowly abundant genes were removed as those <1 mean raw count or <1 mean count per million (CPM) across the dataset
Differential gene expression was calculated using the voom function in limma
Assembly: GRCm38
Supplementary files format and content: Text file containing STAR gene count values

molecule: polyA RNA

organism: Mus musculus

source_name: Non-infected Dp16 murine tracheal epithelial cells

growth_protocol: Epithelial cells were grown and differentiated using culture media previously described in literature (PMID: 12388377; PMID: 12959927; PMID: 21372793; PMID: 23522475)

extract_protocol:
Differentiated epithelial cells were lysed in situ for RNA extraction and subsequent bulk RNA analysis
RNA libraries were prepared for sequencing using standard Nugen Universal Plus mRNA-Seq kit

treatment_protocol: The murine tracheal epithelial cells were divided into two groups: (1) unchallenged (non-infected) and (2) challenged with influenza strain A/Puerto Rico/8/1934 H1N1 (IAV PR8). Once mTEC cultures were fully confluent and well-differentiated (ALI day 21 or later), the epithelial cells were infected at multiplicity of infection of 0.1 with IAV PR8 diluted in culture medium. Viral inoculum was added to the apical chamber of each transwell (into the insert and over the luminal surface ciliated mTECs) at 200 µl inoculum per insert. The cells were then incubated for two hours at 35oC to allow adsorption of virus to the cells. After 2 hours, the inoculum was removed and TWIs were washed once with 200 µl of culture medium and the infected cells were returned to culture at 37oC, 5% CO2 in a humidified chamber. 48 hrs post-inoculation, apical washed were performed to removed released viral particles. The cells were then lysed for RNA extraction for bulk RAN-seq analysis.

tag: treatment

value: Noninfected

tag: genotype


title: GCB STAT3 replicate 2

city: Chicago

first: Mark

last: Maienschein-Cline

state: IL

address: 900 S Ashland Ave, Rm 1058, M/C 937

department: Research Resources Center

institute: University of Illinois at Chicago

library_source: genomic

data_processing:
Raw reads were mapped to the reference genome using BWA MEM. PCR duplicates were removed using Picard MarkDuplicates. Peaks were called using Macs2 against an input sample previously published on GEO (GSM3926504). High-confidence peak calls were determined based on a threshold of 50.
Assembly: mm9
Supplementary files format and content: bed

molecule: genomic DNA

source_name: Mouse splenic germinal center B cells

extract_protocol:
ChIP-seq was performed using the ChIP Assay Kit following the manufacturer’s protocol (Millipore). DNA was sheared to ~200 bp with a Bioruptor sonication water bath for four 15 min cycles consisting of 45s on and 30s off. ChIP was performed with anti-STAT3 (D3Z2G, Cell Signaling Technologies). Resulting DNA was purified with the MinElute PCR Purification Kit (Qiagen). DNA was incubated with a tagmentation enzyme (Illumina) at 37 °C for 30 min and DNA was purified with the MinElute PCR Purification Kit (Qiagen).
DNA libraries were made with the Nextera Indexing kit (Illumina), and DNA was purified with the QiaQuick PCR Purification Kit (Qiagen).

treatment_protocol: Mice were immunized intraperitoneally (i.p.) with 200µg of 4-Hydroxy-3-nitrophenylacetyl-Keyhole Limpet Hemocyanin (NP-KLH, Biosearch Technologies) mixed with Complete Freund’s Adjuvant (CFA, Sigma-Aldrich) on d0 followed by 100µg NP-KLH in Incomplete Freund’s Adjuvant (IFA, Sigma-Aldrich) on d7. Spleens from 10 mice were harvested, processed into single cell suspensions, and pooled on 14d post immunization. Samples were generated from two experiments using a total of 20 mice. Following RBC lysis using Tris-Ammonium Chloride, B cells were first enriched by negative selection (Stem Cell Technologies) and then stained for GC B cell markers viability- , anti-B220 BV605, anti-GL7 FITC and anti-CD95 PE-Cy7. GC B Cells were sorted using a FACS Aria SORP high-performance cell sorter (BD Biosciences) and immediately cross-linked in 37% formaldehyde (Sigma) diluted in PBS (Corning) to a final concentration of 1.5% formaldehyde. Cells were fixed for 12 minutes at 37 C and then reaction was stopped by adding Glycine (Cell Signaling Technologies) and a 10 minute incubation at room temperature. Cells were then washed with PBS and the pellet was snap frozen and stored at -80 C until processing.

tag: tissue/cell type

value: Germinal center B cell

tag: chip antibody

tag: cell type

value: Mouse splenic germinal center B cells


title: SOL_E2F3-mKO_3 [SE3]

city: Bethesda

first: Youngjae

last: Bahn

state: Maryland

address: 10 Center Dr.

institute: NIH

data_processing:
Raw data (raw reads) of FASTQ format were firstly processed through fastp.
Paired-end clean reads were aligned to the reference genome using the Spliced Transcripts Alignment to a Reference (STAR) software.
FeatureCounts was used to count the read numbers mapped of each gene. FPKM (expected number of fragments per kilobase sequence per million base pairs sequenced) of each gene was calculated based on the length of the gene and the reads count mapped to that gene.
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each gene

source_name: Soleus

extract_protocol:
Total RNA was harvested using Trizol reagent. mRNA was further  purified using Invitrogen Dynabeads® mRNA DIRECT™ Kit  (Cat. 610-11). RNA integrity and quantitation were assessed using the RNA Nano 6000 Assay Kit and a Bioanalyzer 2100 system (Agilent Technologies, CA, USA).
RNA libraries were prepared for sequencing using standard Illumina protocols

tag: tissue

value: Skeletal Muscle

tag: tissue type

value: Soleus

tag: strain background

tag: genotype/variation


title: scA1, wt replicate 1, scRNAseq

city: Indianapolis

first: Stephanie

last: Ware

state: IN

address: 1044 W Walnut Street, R4 W227

department: Pediatrics

institute: Indiana University School of Medicine

description: 10x Genomics

library_source: transcriptomic single cell

data_processing:
The demultiplexing, barcoded processing, gene counting and aggregation were made using the Cell Ranger software v3.1.0
Assembly: mm10
Supplementary files format and content: Count matrix files

molecule: total RNA

source_name: whole embryos

extract_protocol:
Embryos were harvested at E8.0 in ice-cold phosphate buffered saline (PBS) containing 10% (v/v) of fetal bovine serum (FBS/PBS). Embryos aged between 0-1 somite were selected for further analysis and the extraembryonic tissue was removed. Embryos were washed with Ca+2/Mg+2- free Dulbecco’s phosphate buffered saline DPBS. Embryos were incubated in TrypLETM Express (Gibco) at 37oC for 6-7 min with intermittent pipetting using wide bore tips. Once embryos were completely dissociated, the enzyme was quenched using 10% FBS/DPBS (Ca+2/Mg2+ free) before the cells were resuspended in Ca2+/Mg2+ free DPBS containing 5% (v/v) endotoxin free FBS. Cell viability (i.e., lack of dead cells), number and size were confirmed with a hemocytometer.
Library was performed according to the manufacter’s instructions (single cell 3’ library v3 protocol, 10x Genomics).

tag: strain

value: C57BL/6 x 129 SvEv

value: whole embryos

tag: developmental stage

value: E.8 (0-2 somites) stage embryos

value: WT


title: RRHFD_3

type: RNA

first: WeiPing

last: Chen

state: MD

address: Bldg 8, Room 1A11, NIDDK/NIH

department: GCL

institute: NIDDK/NIH

hyb_protocol: Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip mouse Genome Array (Mouse430). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.

scan_protocol: GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.

data_processing: The data were normalized by RMA with Partek Genomic Suite 6.6

label: biotin

source_name: RRHFD

label_protocol: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).

growth_protocol: Mouse grow in NIDDK animal core facility.

extract_protocol: Qiagen RNEasy was performed according to the manufacturer's instructions.

treatment_protocol: Two types of mouse (Wt vs. Mutant) were treated with HFD (high fat diet), or RD (regular fat) with three replications.

value: skeletal muscle

value: HY

value: Cdk4-E2F3 mutant

tag: diet

value: HFD


title: 293T cells,RSL3,4h

city: shanghai

first: changzhou

last: feng

address: No. 197, Ruijin 2nd Road

country: China

institute: Shanghai Institute of Hematology, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine

data_processing:
Basecalls performed using CASAVA version 1.8
Adapters and low-quality read ends were removed using Trimmomatic, based on quality control statistics generated by FastQC.
Quality filtered reads were then mapped to GRCh38 using Hisat2
The expression level of each transcript was calculated according to the fragments per kilobase of exon per million mapped reads (FRKM) . StringTie was used to quantify gene and isoform abundances.
R statistical package software EdgeR (Empirical analysis of Digital Gene Expression in R) was utilized for differential expression analysis.
Assembly: GRCh38
Supplementary files format and content: tab-delimited text files include RPKM values for each Sample

organism: Homo sapiens

growth_protocol: 293T cells were maintained in DMEM supplemented with 10% fetal bovine serum (FBS) and antibiotics in humidified atmosphere with 5% CO2 at 37C

extract_protocol:
RNA was extracted using TRIzol reagent (Invitrogen, 15596018).
Isolation of mRNA was performed using the NEBNext PolyA mRNA Magnetic Isolation Module. TruSeq RNA preparation kit (KAPA) was used with 1 ug of total RNA for the construction of sequencing libraries.

treatment_protocol: 293T cells treated with DMSO, EC359(0.5μM), and RSL3(0.5μM) alone, or co-treated with Fer-1(2μM).

tag: cell line

value: Human embryonic kidney


title: HFDWT_3

source_name: HFDWT

value: wildtype


title: DP16_Noninfected, rep 2


title: RDWT_3

source_name: RDWT

value: RD


title: RRRD_3

source_name: RRRD


title: RRHFD_2


title: scD2, wt replicate 3, scRNAseq

description: 10x Genomics


title: RRRD_2


title: DP16_Noninfected, rep 3


title: HFDWT_2


title: SOL_E2F3-mKO_2 [SE2]


title: LB4067T

last: Sun

state: California

description: 10x Genomics single cell barcoded mRNA from CD45+ fraction, Sample #14

value: metastatic brain tumors

value: prior immune checkpoint blockade therapy

value: 3' sequencing

value: dissociated CD45+ single cells


title: RDWT_2


title: DP16_Noninfected, rep 1

value: Noninfected


title: LB4022T

description: 10x Genomics single cell barcoded mRNA from CD45+ fraction, Sample #8

value: no prior immune checkpoint blockade therapy


title: SOL_E2F3-mKO_1 [SE1]

value: Skeletal Muscle

value: Soleus


title: RRHFD_1


title: DP16_Infected, rep 6

source_name: IAV-infected Dp16 murine tracheal epithelial cells

value: Infected


title: LB4104T

description: 10x Genomics single cell barcoded mRNA from CD45+ fraction, Sample #9


title: RRRD_1


title: SOL_MLC-Cre_3 [SC3]

value: MLC-Cre


title: LB4071T

description: 10x Genomics single cell barcoded mRNA from CD45+ fraction, Sample #15


title: SOL_MLC-Cre_2 [SC2]


title: DP16_Infected, rep 5


title: HFDWT_1

value: skeletal muscle


title: LB3976T

description: 10x Genomics single cell barcoded mRNA from CD45+ fraction, Sample #13


title: LB3985T

description: 10x Genomics single cell barcoded mRNA from CD45+ fraction, Sample #7


title: M43

city: Dublin

first: Mona

last: Heiland

address: 123 St. Stephen's green

country: Ireland

institute: RCSI

data_processing:
Sequence reads were trimmed for adaptor sequence/low-quality sequence using Chimera website
Reads were size selected using the Chimera website
Trimmed sequence reads were aligned to miRNBAse sequences using BLASTn
EdgeR and Limma packages from R/Bioconductor were used for the downstream analysis to identify differentially expressed (DE) miRNAs
data was normalised to library sizes (sequencing depth)
Assembly: mm10
Supplementary files format and content: excel file including raw librariy size and miRNA counts for each sample (tech_info_raw)
Supplementary files format and content: excel file including normalised miRNA expression for each sample (normalised-miRNA-espression)
Supplementary files format and content: excel file including one tab with fold change and p values for all detected miRNAs between vehicle and CBD group, second tab has Fold change and p-values for all DE expressed miRNAs between vehicle and CBD group (DE miRNA vehicle vs CBD)

source_name: Mouse, right ventral hippocampus, CBD 200mg/kg/day

extract_protocol:
Total RNA was extracted using the Trizol method. 1 ug of total RNA was used for the construction of sequencing libraries.
RNA libraries for small RNA-seq were prepared using Illumina TruSeq small RNA Library Prep Kit  following manufacturer's protocols. After libraries were purified using Pippin Prep, quality was checked on a bioanalyzer

treatment_protocol: CBD (200mg/kg/day) or vehicle i.p. twice daily for 5 days

value: ventral hippocampus

tag: gender

value: male

value: CBD


title: M41

source_name: Mouse, right ventral hippocampus, vehicle

value: vehicle


title: LB3955T

description: 10x Genomics single cell barcoded mRNA from CD45+ fraction, Sample #6


title: SOL_CDK4KO _3 [SN3]


title: RDWT_1

value: HY

value: wildtype


title: M42


title: DP16_Infected, rep 3


title: DP16_Infected, rep 2


title: LB3935T

description: 10x Genomics single cell barcoded mRNA from CD45+ fraction, Sample #5

value: metastatic brain tumors


title: SOL_CDK4KO _2 [SN2]


title: SOL_MLC-Cre_1 [SC1]


title: M40


title: DP16_Infected, rep1


title: DP16_Infected, rep 4


title: M39


title: LB3912T

description: 10x Genomics single cell barcoded mRNA from CD45+ fraction, Sample #12

value: prior immune checkpoint blockade therapy


title: SOL_CDK4KO _1 [SN1]


title: LB3899T

description: 10x Genomics single cell barcoded mRNA from CD45+ fraction, Sample #11

value: 3' sequencing


title: M27


title: LB3894T

description: 10x Genomics single cell barcoded mRNA from CD45+ fraction, Sample #4

value: dissociated CD45+ single cells


title: M26


title: SOL_CDK4RR _2 [SR2]


title: LB3878T

description: 10x Genomics single cell barcoded mRNA from CD45+ fraction, Sample #3


title: LB3858T

description: 10x Genomics single cell barcoded mRNA from CD45+ fraction, Sample #2


title: M23


title: M22


title: SOL_Wild Type _3 [SW3]

value: Wild Type


title: LB3855T

description: 10x Genomics single cell barcoded mRNA from CD45+ fraction, Sample #1

value: no prior immune checkpoint blockade therapy


title: SOL_Wild Type _2 [SW2]

value: Skeletal Muscle


title: M20


title: TSC2ko_plastic_rapamycin_rep2 [Pietrobon_S16]

city: Ottawa

first: Theodore

last: Perkins

state: ON

address: 501 Smyth Rd.

department: Cellular and Molecular Medicine

country: Canada

institute: Ottawa Hospital Research Institute

data_processing:
Processed raw sequences with fastp to perform automatic adapter trimming and default sequence quality filtering (>60% of bases with Q≥15, and no more than 5 'N' basecalls)
Quantified transcripts using salmon v1.4.0 with --gcBias and --validateMappings flags against an index constructed from GENCODE v35 trancripts plus Lexogen SIRV Set 3 spike-in sequences and genomic decoys
Read transcript quantification data with tximport library and summarized to gene level quantification
Normalized raw read counts using DESeq2 median of ratios method
Scaled normalized raw read counts using DEseq2 regularized log2 transformation
Genome_build: GRCh38
Supplementary_files_format_and_content: salmon transcript quantification: Quantifrication of GENCODE v35 transcripts using salmon v1.3.0
Supplementary_files_format_and_content: Comma separated matrix table includes data for every gene and every sample in three formats: 1) raw gene counts (e.g. used differential expression analysis), 2) normalized counts by DEseq2 median of ratios method (e.g. used for individual gene count visualization), 3) log transformed and regularized counts (e.g. used for dimensionality reduction and heatmap).

extract_protocol:
Custom TriZOL extraction with column purification- see manuscript for details.
RNA libraries were prepared with the SMART-Seq v4 kit (Takara Bio).

value: teratoma-derived smooth muscle cells

tag: culture substrate

value: plastic

value: 20nM rapamycin, 72 hrs


title: control-II

city: nagakute

first: Yinzhi

last: Lin

state: Aichi

address: 1-1 Yazakokarimata

department: Microbiology and Immunology

country: Japan

institute: Aichi Medical University School of Medicine

description: Gene expression in HESC cells of group 2

hyb_protocol: 1.65 ug of Cy3-labelled cRNA (specific activity >10.0 pmol Cy3/ug cRNA) was fragmented at 60°C for 30 minutes according to the manufacturers instructions. After the fragmentation reaction, equal amount of Agilent hybridization buffer was added to the fragmentation mixture and hybridized to Agilent Whole Human Genome Oligo Microarrays (G2519F-026652) for 17 hours at 65Â°C in a rotating Agilent hybridization oven.  After hybridization, microarrays were washed for 1 minute at room temperature with GE Wash Buffer 1 (Agilent), for 1 minute with 37Â°C GE Wash buffer 2 (Agilent), and then dried for 1 minute at room temperature with acetonitrile.

scan_protocol: Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 4x44k array slides (Scan Area 61x21.6 mm, Scan resolution 5 um, Dye channel is set to Green and Green PMT is set to 100%).

data_processing: The scanned images were analyzed with Feature Extraction Software 11.0.1.1 (Agilent) using default parameters (protocol GE1_1100_Jul11 and Grid: 026652_D_F_20150612) to obtain background subtracted and spatially detrended Processed Signal intensities.  Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.

source_name: control

label_protocol: Cyanine-3 (Cy3) labeled cRNA was prepared from 0.2 ug RNA using the Low Input Quick Amp Labeling Kit  (Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA).  Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.

growth_protocol: HESC cells  were provided by the Applied Biological Materials lnc. and were grown in Prigrow lV medium containing 10% charcoal-stripped fetal bovine serum (FBS) at 37 °C in a 5% CO2 incubator.

extract_protocol: RNA was prepared using RNeasy Mini Kit (Qiagen) following the manufacturer's recommendations. RNA concentration was quantified using a NanoDrop-1000 spectrophotometer.

treatment_protocol: HESC cells  were plated at 80% confluence and treated with DHMEQ at 3 μg/ml for 24 h.

value: Human Endometrial Stromal Cells [HESCs]

value: female

tag: age

value: Adult

value: control


title: QA_E2F3-mKO_3 [QE3]

source_name: Quadriceps

value: Quadriceps


title: M19

value: ventral hippocampus

value: male

value: CBD


title: DHMEQ-II

description: Gene expression in HESC cells treated with DHMEQ of group 2

source_name: DHMEQ

value: DHMEQ at 3 μg/ml for 24 h


title: DHMEQ-I

description: Gene expression in HESC cells treated with DHMEQ of group 1


title: control-I

description: Gene expression in HESC cells of group 1


title: SOL_CDK4RR _3 [SR3]

value: Soleus


title: QA_E2F3-mKO_2 [QE2]


title: M18

value: wildtype


title: SOL_CDK4RR _1 [SR1]


title: WT_plastic_vehicle_rep2 [Pietrobon_S13]

value: wild type

value: vehicle


title: M15


title: TSC2ko_hydrogel_rapamycin_rep2 [Pietrobon_S12]

value: hydrogel


title: M14


title: QA_MLC-Cre_3 [QC3]

value: Skeletal Muscle

value: MLC-Cre


title: WT_hydrogel_rapamycin_rep2 [Pietrobon_S11]

value: teratoma-derived smooth muscle cells

value: 20nM rapamycin, 72 hrs


title: M7


title: M21


title: SOL_Wild Type _1 [SW1]

value: Wild Type


title: WT_plastic_rapamycin_rep2 [Pietrobon_S15]

value: plastic


title: TSC2ko_hydrogel_vehicle_rep2 [Pietrobon_S10]


title: QA_MLC-Cre_2 [QC2]


title: QA_CDK4KO _3 [QN3]


title: M6


title: TSC2ko_plastic_vehicle_rep2 [Pietrobon_S14]


title: M3


title: WT_hydrogel_vehicle_rep2 [Pietrobon_S09]


title: QA_CDK4KO _1 [QN1]


title: TSC2ko_plastic_rapamycin_rep1 [Pietrobon_S08]


title: TSC2ko_plastic_vehicle_rep1 [Pietrobon_S06]


title: WT_plastic_vehicle_rep1 [Pietrobon_S05]


title: QA_CDK4KO _2 [QN2]


title: WT_plastic_rapamycin_rep1 [Pietrobon_S07]


title: M1


title: QA_CDK4RR _3 [QR3]


title: TSC2ko_hydrogel_rapamycin_rep1 [Pietrobon_S04]


title: ID-21-i-3

city: Cheras

first: Nur Syakirah

last: Binti Othman

state: Kuala Lumpur

address: Yaacob Latiff Street

department: Physiology

country: Malaysia

institute: National University of Malaysia

data_processing:
Basecalls were performed using bcl2fastq v2.17.1.14 for Novaseq output.
RNA-seq reads were trimmed from 5' or 3' end until the final base had a quality score > 20, using Trimmomatic v0.30, discarding reads left with < 18 bp
RNA-seq reads were mapped to a reference genome (Homo sapiens GRCh38.104) using Bowtie version 2.1.0.
The microRNA differential analysis was performed using the Bioconductor package DESeq2 (V1.6.3)
Assembly: Homo sapiens (GRCh38.104)
Supplementary files format and content: tab-delimited text files include TPM values for each Sample

source_name: HUVEC

growth_protocol: HUVEC were maintained in endothelial cell medium supplemented with 5% fetal bovine serum (FBS) and antibiotics in humidified atmosphere with 5% CO2 at 37C

extract_protocol:
RNA was harvested using miRNeasy mini kit (Qiagen). 1 ug of total RNA was used for the construction of sequencing libraries.
Library was performed according to the manufacter’s instructions.

treatment_protocol: Hyperglycemic condition was induced by treatment of 24 hours after HUVEC reach 80% confluency  with 33.3 mmol/L D-glucose

value: HUVEC

value: High glucose

tag: time

value: 24 hours


title: ID-22-2

tag: treatment


title: QA_CDK4RR _2 [QR2]

value: Quadriceps


title: ID-21-i-2

value: Control


title: QA_MLC-Cre_1 [QC1]


title: QA_CDK4RR _1 [QR1]


title: QA_Wild Type _3 [QW3]


title: M2

value: ventral hippocampus

value: male

tag: genotype

value: CBD


title: TSC2ko_hydrogel_vehicle_rep1 [Pietrobon_S02]


title: ID-22-3


title: WT_hydrogel_vehicle_rep1 [Pietrobon_S01]

value: wild type


title: QA_Wild Type _2 [QW2]


title: QA_E2F3-mKO_1 [QE1]


title: ID-H-3


title: QA_Wild Type _1 [QW1]


title: ID-H-2


title: WT_hydrogel_rapamycin_rep1 [Pietrobon_S03]


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-4-C10-A8

status: Public on Jul 05 2023

city: La Jolla

first: Joseph

middle: R

last: Ecker

state: CA

address: 10010 North Torrey Pines Road

department: Genomic Analysis Laboratory

institute: HHMI-Salk-Institute

data_processing:
Reads trimed with Cutadapt for adapters and low quality bases (Phred score >= 20)
The resulting files are mapped to in-silico bisulfite converted reference genome using Bismark.
The raw BAM file will be filtered using samtools to filter reads by MAPQ > 10 and use Picard to remove PCR duplicates
Tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls will be generated with in-house developing package using the filtered BAM files
Tab-delimited files (HIC files) describing the chromatin contacts will be generated with in-house developing package using the filtered BAM files
Assembly: hg38-donor-snp-substituted
Supplementary files format and content: tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls. Columns are: 1. Chromosome, 2. Position, 3. Strand, 4. Cytosin Context, 5. methylated base call, 6. total mapped base call, 7. not used, place holder column
Supplementary files format and content: tab-delimited files (HIC files) describing the chromatin contacts. Columns are: 1. Strand1, 2. Chromosome1, 3. Position1, 4. place holder column, always be 0, 5. Strand2, 6. Chromosome2, 7. Position2, 8. place holder column, always be 1.
Library strategy: snm3C-seq

extract_protocol:
Regions of human brains were dissected.
Library prepared following protocol discribed in Dong-Sung Lee, Chongyuan Luo, Jingtian Zhou, Sahaana Chandran, Angeline Rivkin, Anna Bartlett, Joseph R. Nery, Conor Fitzpatrick, Carolyn O’Connor,Jesse R. Dixon, Joseph R. Ecker. 2019. “Simultaneous profiling of 3D genome structure and DNA methylation in single human cells.” Nat Methods. 16(10): 999–1006.

tag: brain region

value: M


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-4-C10-C20


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-4-C10-A7


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-4-C10-C7


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-4-C10-B7


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-4-C10-A20


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-4-C10-D19


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-P17


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-P5


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-4-C10-B20


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-4-C10-C8


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-4-C10-B8


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-P18


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-4-C10-B19


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-4-C10-A19


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-O6


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-4-C10-C19


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-O17


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-N6


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-O5


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-N17


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-N18


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-N5


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-M6


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-P6


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-M5


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-M17


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-M18


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-L5


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-L6


title: WT_Infected, rep 1

source_name: IAV-infected wild-type murine tracheal epithelial cells

value: Infected

value: WT


title: WTEB_scRNA_2

city: Shanghai

first: Jiwei

address: 130 DongAn Road

institute: Fudan University

description: 10x Genomics

data_processing:
scRNA-seq reads were aligned and counted by Cell Ranger v7.1.0 with default parameters. Filtering, clustering, and assigning cell type identity to clusters were performed using R package Seurat v4.3.0. Briefly, genes expressed in less than 10 cells and cells in which total UMI < 500 or total expressed genes < 500 were removed. Gene counts were normalized by NormalizeData function. 2,000 highly variable genes were selected in each sample based on a variance stabilizing transformation performed by FindVariableFeatures function. Next, expression matrices were scaled and centered followed by principal component analysis (PCA) for dimensional reduction. PC1 to PC30 were used to construct nearest neighbor graphs in the PCA space (FindNeighbors function) followed by Louvain clustering to identify clusters (resolution = 0.4, FindClusters function). Then, cell clusters were assigned to ESCs, endoderm cells, mesoderm cells and ectoderm cells by their canonical gene markers.
Assembly: mm9
Supplementary files format and content: barcode.tsv, genes.tsv and matrix.mtx to generate genexcells matrix

source_name: Embryoid bodies

extract_protocol:
Day 6 EBs were disscoiated by TrypLE and dissociated cells were resuspended in PBS+ 0.04%BSA. The quantity and quality of the cells were accessed by Acridine Orange and Propidium iodide dye on a Cellometer Auto 2000 (Nexcelom).
16,000 cells were loaded on a 10x Chromium Controller based on 10x Chromium Single Cell 3' Library manual (10x Genomics). After cell partitioning and GEM generation, reverse transcription was performed, and cDNA was pooled and cleaned up by beads. cDNA was further amplified and cleaned up by SPRI beads (Beckman). The quality of cDNA was assessed by High Sensitivity D5000 Tapestation (Agilent Technologies) and quantified by Qubit 2.0 DNA HS assay (ThermoFisher). 3’ Gene expression library prep was carried out according to the 10x Chromium Next GEM Single Cell 3' v3.1 manual.

value: Embryoid bodies


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-L18


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-L17


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-J6


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-J17


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-K5


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-O18


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-J18


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-K17


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-I5


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-I6


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-H6


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-K18


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-I18


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-I17


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-H5


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-G5


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-H17


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-H18


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-G18


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-K6


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-J5


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-G17


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-F6


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-F17


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-E18


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-E6


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-E5


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-F18


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-E17


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-D6


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-D17


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-D18


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-C6


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-C5


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-C18


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-B5


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-G6


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-B17


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-B18


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-C17


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-A6


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-A5


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-A18


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-A17


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-F5


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-P16


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-P4


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-P15


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-D5


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-O4


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-O3


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-3-C10-B6


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-N15


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-O15


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-M4


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-N16


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-M3


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-K16


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-N3


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-K3


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-L4


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-L15


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-N4


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-M15


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-O16


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-K4


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-J3


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-L3


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-J4


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-M16


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-K15


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-J15


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-J16


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-I4


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-I3


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-I16


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-H3


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-I15


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-H4


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-H15


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-H16


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-G4


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-G3


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-F3


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-F15


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-F16


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-G16


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-E15


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-E4


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-D3


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-E16


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-D16


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-E3


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-C3


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-C4


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-D15


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-C16


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-B3


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-B16


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-B4


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-B15


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-A4


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-A3


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-A15


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-A16


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-P2


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-G15


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-D4


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-P13


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-O2


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-2-C10-C15


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-O1


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-O14


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-P1


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-N13


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-O13


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-N2


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-N1


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-M2


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-M1


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-L2


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-M14


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-L13


title: ATAC_seq_TCF1KO_LSK_P45_DN1_Rep2B_Rep2T

city: Philadelphia

first: Naomi

last: Goldman

state: PA

address: 324 BRB II/III, 421 Curie Blvd

institute: University of Pennsylvania

data_processing:
ATAC-seq libraries were pair-end sequenced by Illumina NextSeq 500. BWA was used for alignment. Reads aligned to the mitochondrial genome or chrY as well as reads mapping to multiple genomic loci were discarded from downstream analyses. Additionally, Picard was used to mark and remove duplicates. Furthermore, for each ATAC-seq library the insert size was calculated by Picard.
Assembly: GRCm38
Supplementary files format and content: Bigwig files were generated by bedtools genomecov and wigToBigWig normalizing tracks to tags-per-million

source_name: BM derived CD44+ CD25- DN1

extract_protocol:
ATAC-seq was performed as previously described with minor modifications (Buenrostro et al., 2013). Fifty thousand cells were pelleted at 550 x g and washed with 1 mL 1x PBS, followed by treatment with 50 μL lysis buffer (10 mM Tris-HCl [pH 7.4], 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630). After pelleting nuclei, the pellets were resuspended in 50 μL transposition reaction with 2.5 μL Tn5 transposase (FC-121-1030; Illumina). The reaction was incubated in a 37°C water bath for 45 minutes. Tagmented DNA was purified using a MinElute Reaction Cleanup Kit (Qiagen) and amplified with varying cycles, depending on the side reaction results. Libraries were purified using a QIAQuick PCR Purification Kit (Qiagen).
Libraries were paired-end sequenced (38bp+38bp) on a NextSeq 550 (Illumina). Libraries were validated for quality and size distribution using a TapeStation 2200 (Agilent).

treatment_protocol: Transduction of Tcf7 KO scid.adh cells was accomplished by addition of retroviral supernatants to culture medium supplemented with polybrene (8mg/ml) and spinfected at 1,750 RPM for 25 minute. 72hrs after transduction, live transduced cells were sorted for downstream experiments. Cells from co-cultures were stained with L/D APCef780, and fluorescent antibodies to B220, CD44, CD45, Thy1.2, Ckit, CD25, and CD11b. Sorting was performed on a BD FACSAria after 7 days to isolate DN1 (CD45+c-KithiCD44hI CD25low) and DN2 cells (CD45+c-KitlowCD44low CD25high).

value: Tcf7eGFP fl/fl Cre+

value: Cultured on OP9-DLL1


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-L14


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-L1


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-P14


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-K2


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-K13


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-K14


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-K1


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-J2


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-J14


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-J13


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-J1


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-I2


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-N14


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-I13


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-H2


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-H14


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-G14


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-H1


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-M13


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-I1


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-G1


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-G13


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-F13


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-E2


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-F1


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-H13


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-F14


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-E13


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-E14


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-E1


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-F2


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-D2


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-D14


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-D13


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-C2


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-D1


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-C14


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-C13


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-B2


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-I14


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-C1


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-G2


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-B13


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-B14


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-A13


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-A2


title: HBA_211015_H1930001_CX57_V1C_3C_1_P6-6-I2-P12


title: HBA_211015_H1930001_CX57_V1C_3C_1_P6-6-I2-P24


title: HBA_211015_H1930001_CX57_V1C_3C_1_P7-1-C10-A1


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