title: 7F_M_2860


title: 7F_M_2840

status: Public on Jul 13 2023

type: SRA

city: La Jolla

first: Joseph

middle: R

last: Ecker

state: CA

address: 10010 North Torrey Pines Road

department: Genomic Analysis Laboratory

country: USA

institute: HHMI-Salk-Institute

library_source: genomic

data_processing:
Reads trimed with Cutadapt for adapters and low quality bases (Phred score >= 20)
The resulting files are mapped to in-silico bisulfite converted mm10 reference genome using Bismark.
The raw BAM file will be filtered using samtools to filter reads by MAPQ > 10 and use Picard to remove PCR duplicates
Tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls will be generated with in-house developing package using the filtered BAM files
Assembly: mm10
Supplementary files format and content: tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls. Columns are: 1. Chromosome, 2. Position, 3. Strand, 4. Cytosin Context, 5. methylated base call, 6. total mapped base call, 7. not used, place holder column
Library strategy: snmC-seq2

molecule: genomic DNA

organism: Mus musculus

source_name: C57BL/6J Male P56 Mouse Brain

growth_protocol: All mice are C57BL/6J Male, brain dissected in P56

extract_protocol:
the brain of P56 male C57BL/6J mouse will be removed and cut into 600 microns thick coronal sections using a tissue matrix. Then each region will be microdissected from the coronal tissue sections based on identified landmarks and brain atlas coordinates, as defined in Allen brain atlas CCF3 (Avaliable at: http://atlas.brain-map.org). For each dissection region, the tissue from several littermate mice will be combined together into to separate replicate groups. The number of animals in a replicate depends on the size of the tissue. Nuclei preparation will be stained with the fluorescently labeled anti-NeuN body and nucleic acid stain Hoechst 33342.
Library prepared following protocol discribed in Luo, Chongyuan, Angeline Rivkin, Jingtian Zhou, Justin P. Sandoval, Laurie Kurihara, Jacinta Lucero, Rosa Castanon, et al. 2018. “Robust Single-Cell DNA Methylome Profiling with snmC-seq2.” Nature Communications 9 (1): 3824.

tag: brain region

value: GP

tag: age

tag: strain


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title: 7E_M_1927


title: 7E_M_1934


title: 7E_M_1922


title: 7E_M_1945


title: 7E_M_1950


title: 7E_M_1926


title: 7E_M_1924


title: 7E_M_1921


title: 7E_M_1920


title: 7E_M_1923


title: 7E_M_1919


title: 7E_M_1918


title: 7E_M_1917


title: 7E_M_1916


title: 7E_M_1931


title: 7E_M_1932


title: 7E_M_1943


title: 7E_M_1933


title: 7E_M_1914


title: 7E_M_1849


title: 7E_M_1915


title: 7E_M_1847


title: 7E_M_1848


title: 7E_M_1845


title: 7E_M_1846


title: 7E_M_1843


title: 7E_M_1842


title: 7E_M_1840


title: 7E_M_1837


title: 7E_M_1838


title: 7E_M_1841


title: 7E_M_1844


title: 7E_M_1835


title: 7E_M_1836


title: 7E_M_1834


title: 7E_M_1831


title: 7E_M_1830


title: 7E_M_1832


title: 7E_M_1833


title: 7E_M_1829


title: 7E_M_1828


title: 7E_M_1827


title: 7E_M_1850


title: 7E_M_1825


title: 7E_M_1823


title: 7E_M_1824


title: 7E_M_1822


title: 7E_M_1839


title: 7E_M_1821


title: 7E_M_1819


title: 7E_M_1816


title: 7E_M_1818


title: 7E_M_1812


title: 7E_M_1814


title: 7E_M_1817


title: 7E_M_1813


title: 7E_M_1811


title: 7E_M_1826


title: 7E_M_1820


title: 7E_M_1810


title: 7E_M_1808


title: 7E_M_1806


title: 7E_M_1805


title: 7E_M_1809


title: 7E_M_1803


title: 7E_M_1807


title: 7E_M_1804


title: 7E_M_1802


title: 7E_M_1799


title: 7E_M_1801


title: 7E_M_1798


title: 7E_M_1797


title: 7E_M_1795


title: 7E_M_1800


title: 7E_M_1794


title: 7E_M_1793


title: 7E_M_1792


title: 7E_M_1796


title: 7E_M_149


title: 7E_M_153


title: 7E_M_164


title: 7E_M_155


title: 7E_M_159


title: 7E_M_163


title: 7E_M_148


title: 7E_M_147


title: 7E_M_152


title: 7E_M_162


title: 7E_M_146


title: 7E_M_161


title: 7E_M_150


title: 7E_M_151


title: 7E_M_142


title: 7E_M_157


title: 7E_M_144


title: 7E_M_143


title: 7E_M_145


title: 7E_M_160


title: 7E_M_165


title: 7E_M_167


title: 7E_M_156


title: 7E_M_141


title: 7E_M_168


title: 7E_M_166


title: 7E_M_138


title: 7E_M_139


title: 7E_M_158


title: 7E_M_140


title: 7E_M_135


title: 7E_M_132


title: 7E_M_127


title: 7E_M_130


title: 7E_M_131


title: 7E_M_133


title: 7E_M_134


title: 7E_M_136


title: 7E_M_137


title: 7E_M_128


title: 7E_M_60


title: 7E_M_53


title: 7E_M_56


title: 7E_M_57


title: 7E_M_54


title: 7E_M_59


title: 7E_M_55


title: 7E_M_61


title: 7E_M_129


title: 7E_M_58


title: 7E_M_62


title: 7E_M_50


title: 7E_M_47


title: 7E_M_52


title: 7E_M_46


title: 7E_M_45


title: 7E_M_51


title: 7E_M_38


title: 7E_M_40


title: 7E_M_33


title: 7E_M_49


title: 7E_M_48


title: 7E_M_28


title: 7E_M_34


title: 7E_M_27


title: 7E_M_29


title: 7E_M_26


title: 7E_M_31


title: 7E_M_30


title: 7E_M_24


title: 7E_M_43


title: 7E_M_44


title: 7E_M_41


title: 7E_M_42


title: 7E_M_23


title: 7E_M_19


title: 7E_M_39


title: 7E_M_18


title: 7E_M_13


title: 7E_M_16


title: 7E_M_32


title: 7E_M_35


title: 7E_M_36


title: 7E_M_11


title: 7E_M_25


title: 7E_M_8


title: 7E_M_4


title: 7E_M_22


title: 7E_M_21


title: 7E_M_17


title: 7E_M_14


title: 7E_M_3


title: 7E_M_20


title: 7E_M_12


title: 7E_M_15


title: 7E_M_7


title: 7E_M_2


title: 7E_M_10


title: 7E_M_9


title: 7E_M_0


title: 7E_M_1


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S36_TAGCTT

city: Philadelphia

first: Hao

last: Wu

state: PA

address: 415 Curie Boulevard

department: Genetics

institute: University of Pennsylvania

data_processing:
The pre-processing (read alignment, quality filtering and read deduplication) was performed for snhmC/Joint-snhmC-seq datasets of individual single cells as previously described with minor modifications6. Briefly, demultiplexing of inline barcodes was first performed allowing up to 1-nt mismatch. The data quality was examined with FastQC (http://www.bioinformatics.babraham.ac.uk/projects/fastqc/). Raw sequencing reads were trimmed for adaptor sequences and inline barcodes using Cutadapt with the following parameters in paired-end mode: -f fastq -q 20 -u 16 -U 16 -m 30 -a AGATCGGAAGAGCACACGTCTGAAC -A AGATCGGAAGAGCGTCGTGTAGGGA. The trimmed R1 and R2 reads were mapped independently against the reference genome (mm10) using Bismark (v0.18.2) with following parameters: --bowtie2 -D 15 -R 2 -L 20 -N 0 –score_min L,0,-0.2 (--pbat option was turned on for mapping R1 reads). Uniquely mapped reads were filtered for minimal mapping quality (MAPQ>=10) using samtools9. PCR duplicates were removed using the Picard MarkDuplicates (http://broadinstitute.github.io/picard/). To eliminate reads from strands not deaminated by A3A, reads with three or more consecutive non-converted cytosines in the CH context were removed using filter_non_conversion in Bismark. Low quality snhmC/Joint-snhmC-seq datasets were removed using the following set of criteria. First, Mapping rate of raw sequencing reads is set to be at least 20%; Second, we set a minimum on the number of pass filter reads (uniquely mapped, deduplicated, MAPQ>=10, <=3 consecutive 5mCH sites) to be 100,000 per cell; Third, the CH deamination rate in spike-in lambda phage control is higher than 98%. Base calling of unmethylated and methylated cytosines was performed by bismark_methylation_extractor in Bismark in each individual nucleus. 5mC+5hmC signals were calculated as % of C/(C+T) at each cytosine base.
Assembly: mm10
Supplementary files format and content: Tab-delimited bismark methylation calling files include genomic coordinates (columan 1-3: chromosome, start, and end), 5mC+5hmC signal (column 4: % of 5mC+5hmC levels normalized to total reads), 5mC+5hmC modified read (column 5), and unmodified read (column 6) for all detected CpG (._CpG.mm10.bismark.cov)  or CpH (._CpH.mm10.bismark.cov) sites across the mouse genome in individually sequenced cell.

source_name: Cortex

growth_protocol: All experiments were conducted in accordance with the guidelines of the Institutional Animal Care and Use Committee (IACUC) at the University of Pennsylvania. Mice were group-housed in cages of three to five in a 12-hr light/dark cycle with food and water provided ad libitum. All mice used for experiments were naïve to behavioral assays and other procedures. For all bulk-nucleus and single-nucleus experiments, 6-10 weeks old, wild-type, male mice in a pure C57BL/6J background were used. For prospectively isolating inhibitory (Inh) and excitatory (Ex) neuronal subtypes to benchmark snhmC-seq and joint-snhmC-seq, 9-week-old transgenic mice (Mecp2Tavi/y, NexCre/+, Rosa26BirA/BirA) from a C57BL/6J background were used as previously described (Johnson et al. 2017; PMID: 28920956).

extract_protocol:
Nuclei isolation from mouse cortical tissues was performed as described with minor modifications (Hu* & Fabyanic* et al., 2017; PMID: 29220646). Briefly, mouse brains were rapidly resected on ice. Cortices were flash-frozen in liquid nitrogen and stored at -80°C before nuclear isolation. Nuclei were isolated and purified as previously described with some modifications3. Briefly, 14mL sucrose cushion (1.8M sucrose (Sigma-Aldrich, RNase and DNase free, ultra-pure grade), 10mM Tris-HCl pH 8.0, 3mM MgAc2 (Sigma-Aldrich), and protease inhibitor cocktail (Roche)) was added to the bottom of centrifuge tubes (Beckman Coulter). Using a glass homogenizer (Wheaton), a frozen mouse cortical sample was dounce homogenized (19 times with loose pestle followed by 4 times with tight pestle) in 12mL homogenization buffer (0.32M sucrose, 5mM CaCl2 (Sigma-Aldrich), 3mM MgAc2, 10mM Tris-HCl pH 8.0, 0.1% Triton X-100 (Sigma-Aldrich), 0.1mM EDTA (Invitrogen), protease inhibitor cocktail (Roche)). Homogenates (~12ml) were carefully layered onto the sucrose cushion in the centrifuge tubes, and 10mL homogenization buffer was added atop of the homogenates. The tubes were then balanced and centrifuged in a Beckman Coulter L7-65 Ultracentrifuge at 82,705 g at 4°C for 2hr using a Beckman Coulter SW28 swing bucket rotor (Beckman Coulter). The supernatant was carefully removed via aspiration. 1ml chilled DPBS was added to resuspend the nuclear pellet, and nuclei were subsequently transferred to a 1.5-mL tube. Nuclei were pelleted at 500 g for 10 min at 4C, and then resuspended in 0.01% BSA (Sigma-Aldrich) in DPBS. After resuspension, nuclei were filtered through a 40-μm cell strainer (Fisher Scientific), visually inspected for morphology and quality assurance, and counted using a Fuchs-Rosenthal counting chamber before FANS.
All steps of library preparation were performed in a horizontal laminar flow hood (AirCleanⓇ600 PCR Workstation) to minimize environmental DNA contamination. To profile both 5hmC and true 5mC in the same single cell through physical splitting, bisulfite conversion of single nuclei was first carried out using the EZ-96 DNA Methylation-Direct™ Kit (Zymo, Deep Well Format, D5023). Briefly, 96-well plates containing FANS-isolated single nuclei were heated at 50°C for 20min and stored in -80°C for later use. 32.5μL CT Conversion Reagent was added to each well, followed by sealing the plate and shaking to mix. Plates were treated with the following program using a thermocycler: two cycles of [98°C for 8min, 64°C for 1h45min], and 4°C forever. Purification was performed using Zymo-Spin™ I-96 Binding Plates preloaded with 250μL M-binding buffer according to kit instructions with following modifications. For the Joint-snhmC-seq, 20µl of nuclease-free water was used as the elution buffer. The 9µl eluent retained for the snmC-seq2/split (bisulfite-only) workflow will proceed directly to random priming. To the other 9μl eluent retained for snhmC-seq2/split workflow, 7µl nuclease-free water was added to the eluant, for a total reaction volume of 16µl. The 16µl of bisulfite-treated DNA were denatured at 95°C for 1min and snap cooled by transfer to an isopropanol dry ice bath and then to the −80°C pre-incubated PCR tube rack. To snap-cooled samples, 8µl of deamination enzyme mix (2.4µl of 10x reaction buffer (50mM Bis-Tris (pH 6.0) with 0.1% Triton X-100), 0.48µl BSA, 0.48µl of commercial A3A (at a final concentration of ~500 nM, NEB, E7125L) or in-house A3A (~1000 nM), and 4.64µL nuclease-free water) was added into each well. The deamination reactions were incubated for 30 minutes at 37°C, tapped to mix, and spun down before undergoing an additional incubation for 2.5h at 37°C. Post-incubation, these reactions were purified with 2x commercial SPRI beads (Beckman Coulter B23318), and eluted in 9µl of Low EDTA TE buffer (IDT). For the Joint-snhmC-seq, P5L random primers were added to split single nuclei samples as described for snhmC-seq. Briefly, 1µl of matched, individual 250µM random primers was added to each reaction (9µl) to allow for subsequent multiplexing (up to 24-plex) and pairing of snmC-seq2/split to snhmC-seq2/split reactions of the same single cell. Plates were then heated at 95°C using a thermocycler for 3min to denature and were immediately chilled on ice for 2min. Next, 10µl of enzyme mix (2µl o f10x Isothermal Amplification Buffer II, 0.8µl of Bst 3.0 DNA Polymerase (8,000 U/ml), 1.2µl 100 mM MgSO4 (these three reagents all from NEB M0374L), 2.8µl of 10mM dNTP (NEB N0447L), and 3.2µl water) was added to each well (a 20µl reaction) and reactions were mixed by shaking. Reactions were then incubated at 60°C for 1h. Following this, 2μL Exonuclease 1 (20U/μl, Enzymatics X8010L) and 1μL Shrimp Alkaline Phosphatase (rSAP) (1U/μl, NEB M0371L) was added to each reaction followed by vortexing and incubation in a thermocycler at 37°C for 30min followed by 4°C forever. To multiplex subsequent adaptor ligation reactions, 18.4μl (0.8x) homebrew SPRI beads were added to each reaction and 8 sample/bead mixtures were pooled (up to 24 samples if all inline barcodes were used). The sample/bead mixtures were incubated at room temperature for 5min before being placed on a 96-well magnetic separator (DynaMag™-96 Side Magnet, ThermoFisher 12331D). Supernatant was removed from each well, followed by three rounds of washing with 180μl 80% ethanol. Beads were air dried at room temperature and 10μl Low EDTA TE buffer (IDT) was added to each well to fully resuspend the beads. Eluted samples were then transferred to either new PCR strips (for bulk sample) or 96-well plates (for single nuclei). The reactions were denatured in a thermocycler at 95°C for 3min and subsequently chilled on ice for 2min. 10.5μl Adaptase master mix (2μl Buffer G1, 2μl Reagent G2, 1.25μl Reagent G3, 0.5μl Enzyme G4, 0.5μl Enzyme G5, and 4.25μl Low EDTA TE buffer from xGenTM Methyl-Seq Lib Prep kit, IDT, 10009860) was added to each reaction, followed by vortexing. Reactions were incubated in a thermocycler at 37°C for 30min then 4°C forever. Finally, to amplify the pooled library and add dual indexing barcodes, 30μl PCR mix (25μl KAPA HiFi HotStart ReadyMix, Roche/KAPA Biosystems KK2602, 1μl of 30μM P5 (D501-508) indexing primer, and 5μl of 10μM P7 (D701-712) indexing primer) were added to each well, followed by mixing with vortexing. Reactions were transferred to a thermocycler programmed with the following stages: 95°C for 2min, 98°C for 30sec, 15 cycles of [98°C for 15sec, 64°C for 30sec, 72°C for 2min] (for pooled single nucleus reactions), 72°C for 5min, and 4°C forever. For bulk samples, we perform qPCR and subtract two cycles from the inflection point for each sample to determine optimal cycle number for amplification. PCR products were cleaned with two rounds of 0.8x SPRI beads, concentration was determined via QbitⓇ dsDNA High Sensitivity Assay Kit (Invitrogen Q32851), and library size was determined via Bioanalyzer (Agilent High Sensitivity DNA Kit, 5067-4626). Reactions were sequenced on Illumina NovaSeq 6000 (300-cycle v1.5 kit) sequencers.

tag: tissue

value: Cortex

tag: developmental stage

value: Postnatal 6-10 weeks

tag: Sex

value: Male

tag: genotype

value: wild-type

tag: treatment


title: 7E_M_6


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S36_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S36_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S36_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S36_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S36_CGATGT


title: 7E_M_5


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S35_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S35_CGATGT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S35_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S35_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S35_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S35_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S35_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S34_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S34_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S34_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S33_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S34_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S33_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S34_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S34_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S33_CGATGT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S34_CGATGT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S33_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S33_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S35_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S33_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S32_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S32_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S32_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S32_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S32_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S32_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S33_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S31_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S31_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S31_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S31_CGATGT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S31_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S31_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S31_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S30_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S30_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S30_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S30_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S30_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S30_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S30_CGATGT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S29_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S30_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S32_CGATGT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S29_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S29_CGATGT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S32_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S29_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S31_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S29_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S29_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S29_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S28_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S28_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S28_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S29_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S28_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S28_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S28_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S27_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S27_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S27_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S26_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S27_CGATGT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S27_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S26_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S26_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S26_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S26_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S25_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S26_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S27_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S25_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S26_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S25_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S28_CGATGT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S28_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S25_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S27_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S25_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S9_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S9_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S26_CGATGT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S9_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S9_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S9_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S9_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S9_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S8_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S8_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S8_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S8_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S7_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S7_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S7_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S25_CGATGT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S25_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S8_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S7_CGATGT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S7_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S7_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S25_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S7_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S7_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S8_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S6_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S6_CGATGT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S6_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S6_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S6_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S6_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S6_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S8_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S5_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S6_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S5_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S5_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S5_CGATGT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S5_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S5_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S4_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S4_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S3_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S3_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S4_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S4_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S4_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S4_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S3_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S3_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S3_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S5_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S3_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S3_CGATGT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S5_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S2_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S2_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S2_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S2_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S3_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S1_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S2_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S1_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S1_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S4_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S12_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S12_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S11_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S12_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S12_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S11_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S1_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S12_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S11_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S11_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S11_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S10_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S1_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S11_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S11_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S10_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S10_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S10_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S1_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S1_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S12_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S10_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S10_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S10_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S36_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S36_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_pos_novaseq06.25.2021_S12_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S36_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S36_CGATGT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S35_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S35_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S35_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S36_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S36_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S35_CGATGT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S35_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S35_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S35_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S36_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S34_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S34_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S34_CGATGT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S35_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S34_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S36_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S33_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S34_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S34_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S33_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S33_CGATGT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S33_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S33_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S34_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S33_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S33_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S32_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S33_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S32_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S32_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S32_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S32_CGATGT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S31_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S31_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S31_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S32_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S32_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S32_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S31_CGATGT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S31_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S30_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S31_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S31_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S31_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S30_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S30_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S30_CGATGT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S30_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S30_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S30_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S29_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S29_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S30_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S29_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S29_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S28_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S28_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S29_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S28_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S29_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S27_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S27_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S27_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S28_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S27_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S28_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S34_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S28_CGATGT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S26_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S27_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S26_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S27_CGATGT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S26_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S27_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S29_CGATGT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S26_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S26_ATCACG


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S26_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S26_CGATGT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S26_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S25_TAGCTT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S25_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S28_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S25_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S25_CTTGTA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S28_ACTTGA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S25_CGATGT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S25_CAGATC


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S27_GCCAAT


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S25_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S9_GCCAAT

value: NEX/Neurod6-Cre reporter


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S9_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S9_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S9_TGACCA


title: snmCseq2.split_Cortex.WT_NeuN_neg_novaseq06.25.2021_S25_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S9_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S9_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S9_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S9_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S8_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S8_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S8_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S8_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S8_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S8_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S8_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S8_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S7_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S7_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S7_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S7_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S7_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S7_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S7_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S6_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S6_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S7_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S6_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S6_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S6_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S6_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S5_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S6_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S5_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S5_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S6_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S4_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S4_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S5_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S5_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S4_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S5_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S4_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S5_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S4_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S4_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S3_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S3_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S3_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S4_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S3_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S3_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S3_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S3_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S5_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S2_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S2_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S4_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S2_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S2_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S2_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S2_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S2_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S2_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S1_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S1_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S1_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S3_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S1_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S1_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S12_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S1_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S1_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S1_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S12_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S12_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S12_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S11_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S12_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S11_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S12_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S11_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S11_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S11_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S11_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S11_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S11_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S10_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S10_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S10_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S10_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S10_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S12_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S36_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S36_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S10_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S36_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S36_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S36_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S36_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S36_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S35_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S36_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S12_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S34_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S35_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S35_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S35_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S35_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S10_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S35_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S35_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S35_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq08.03.2021_S10_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S34_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S34_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S34_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S34_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S34_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S33_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S33_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S33_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S33_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S33_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S33_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S32_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S32_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S32_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S32_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S34_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S32_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S32_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S31_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S32_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S31_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S31_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S34_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S31_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S30_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S31_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S30_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S30_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S30_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S33_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S29_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S33_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S29_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S29_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S29_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S31_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S31_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S29_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S29_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S28_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S29_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S28_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S28_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S28_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S28_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S28_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S30_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S27_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S27_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S27_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S30_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S27_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S27_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S27_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S29_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S27_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S27_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S26_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S26_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S26_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S26_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S25_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S25_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S28_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S25_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S26_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S25_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S25_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S25_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S9_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S9_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S25_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S9_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S9_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S9_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S9_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S9_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S8_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S8_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S26_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S8_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S7_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S25_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S8_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S8_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S8_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Inh_novaseq06.25.2021_S26_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S7_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S8_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S8_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S7_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S7_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S7_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S9_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S6_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S7_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S6_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S6_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S6_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S6_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S5_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S5_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S5_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S5_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S7_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S5_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S5_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S6_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S6_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S5_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S4_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S4_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S4_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S4_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S4_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S3_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S4_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S4_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S4_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S3_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S3_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S3_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S3_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S2_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S2_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S3_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S5_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S3_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S2_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S2_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S2_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S2_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S2_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S2_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S3_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S1_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S1_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S1_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S1_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S1_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S1_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S1_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S12_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S12_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S12_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S1_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S12_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S12_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S12_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S11_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S11_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S11_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S12_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S11_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S11_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S11_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S11_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S10_TGACCA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S11_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S10_GCCAAT


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S10_TAGCTT


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S10_CTTGTA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S10_CAGATC


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S10_CGATGT


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S10_ACTTGA


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S10_ATCACG


title: snmCseq2.split_Cortex.NEXcre_Ex_novaseq06.25.2021_S12_CAGATC


title: snhmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S48_TGACCA

data_processing:
The pre-processing (read alignment, quality filtering and read deduplication) was performed for snhmC/Joint-snhmC-seq datasets of individual single cells as previously described with minor modifications6. Briefly, demultiplexing of inline barcodes was first performed allowing up to 1-nt mismatch. The data quality was examined with FastQC (http://www.bioinformatics.babraham.ac.uk/projects/fastqc/). Raw sequencing reads were trimmed for adaptor sequences and inline barcodes using Cutadapt with the following parameters in paired-end mode: -f fastq -q 20 -u 16 -U 16 -m 30 -a AGATCGGAAGAGCACACGTCTGAAC -A AGATCGGAAGAGCGTCGTGTAGGGA. The trimmed R1 and R2 reads were mapped independently against the reference genome (mm10) using Bismark (v0.18.2) with following parameters: --bowtie2 -D 15 -R 2 -L 20 -N 0 –score_min L,0,-0.2 (--pbat option was turned on for mapping R1 reads). Uniquely mapped reads were filtered for minimal mapping quality (MAPQ>=10) using samtools9. PCR duplicates were removed using the Picard MarkDuplicates (http://broadinstitute.github.io/picard/). To eliminate reads from strands not deaminated by A3A, reads with three or more consecutive non-converted cytosines in the CH context were removed using filter_non_conversion in Bismark. Low quality snhmC/Joint-snhmC-seq datasets were removed using the following set of criteria. First, Mapping rate of raw sequencing reads is set to be at least 20%; Second, we set a minimum on the number of pass filter reads (uniquely mapped, deduplicated, MAPQ>=10, <=3 consecutive 5mCH sites) to be 100,000 per cell; Third, the 5mCG deamination rate in spike-in lambda phage control is higher than 90%. Base calling of unmethylated and methylated cytosines was performed by bismark_methylation_extractor in Bismark in each individual nucleus. 5hmC signals were calculated as % of C/(C+T) at each cytosine base.
Assembly: mm10
Supplementary files format and content: Tab-delimited bismark methylation calling files include genomic coordinates (columan 1-3: chromosome, start, and end), 5hmC signal (column 4: % of 5hmC levels normalized to total reads), 5hmC read (column 5), and unmodified read (column 6) for all detected CpG (._CpG.mm10.bismark.cov)  or CpH (._CpH.mm10.bismark.cov) sites across the mouse genome in individually sequenced cell.
Library strategy: bisulfite-assisted ACE-seq


title: snhmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S48_TAGCTT


title: snhmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S47_GCCAAT


title: snhmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S48_CGATGT


title: snhmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S47_TGACCA


title: snhmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S47_TAGCTT


title: snhmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S48_GCCAAT


title: snhmCseq2.split_Cortex.WT_NeuN_pos_novaseq08.03.2021_S47_CAGATC