title: HBA_211122_H1930002_CX48_LEC_3C_1_P4-5-E22-G9.3C


title: HBA_211122_H1930002_CX48_LEC_3C_1_P4-5-E22-F22.3C

status: Public on Jul 05 2023

type: SRA

city: La Jolla

first: Joseph

middle: R

last: Ecker

state: CA

address: 10010 North Torrey Pines Road

department: Genomic Analysis Laboratory

country: USA

institute: HHMI-Salk-Institute

library_source: genomic

data_processing:
Reads trimed with Cutadapt for adapters and low quality bases (Phred score >= 20)
The resulting files are mapped to in-silico bisulfite converted reference genome using Bismark.
The raw BAM file will be filtered using samtools to filter reads by MAPQ > 10 and use Picard to remove PCR duplicates
Tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls will be generated with in-house developing package using the filtered BAM files
Tab-delimited files (HIC files) describing the chromatin contacts will be generated with in-house developing package using the filtered BAM files
Assembly: hg38-donor-snp-substituted
Supplementary files format and content: tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls. Columns are: 1. Chromosome, 2. Position, 3. Strand, 4. Cytosin Context, 5. methylated base call, 6. total mapped base call, 7. not used, place holder column
Supplementary files format and content: tab-delimited files (HIC files) describing the chromatin contacts. Columns are: 1. Strand1, 2. Chromosome1, 3. Position1, 4. place holder column, always be 0, 5. Strand2, 6. Chromosome2, 7. Position2, 8. place holder column, always be 1.
Library strategy: snm3C-seq

molecule: genomic DNA

organism: Homo sapiens

extract_protocol:
Regions of human brains were dissected.
Library prepared following protocol discribed in Dong-Sung Lee, Chongyuan Luo, Jingtian Zhou, Sahaana Chandran, Angeline Rivkin, Anna Bartlett, Joseph R. Nery, Conor Fitzpatrick, Carolyn O’Connor,Jesse R. Dixon, Joseph R. Ecker. 2019. “Simultaneous profiling of 3D genome structure and DNA methylation in single human cells.” Nat Methods. 16(10): 999–1006.

tag: brain region

value: LEC

tag: gender

value: M


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