title: PAPS 6

description:
smallRNA 50nts
SAP6_35468_GCCAAT

value: PAPS patient


title: HZ62_silique_H3K4me3_BHCS-196

status: Public on Jul 18 2023

type: SRA

city: Wuhan

first: Wenying

last: Zhong

address: Lion Rock Street

country: China

institute: Huazhong Agricultural University

library_source: genomic

data_processing:
Fastp with default parameters was used to trimmed adaptors and low-quality reads in raw reads
Clean reads were mapped to the Brassica napus ZS11 genome by BWA-MEM with default parameters
SAMtools was used to remove the low mapping quality reads with parameters “-q 30 -f 2”，GATK MarkDuplicates was used to rm duplicated reads
ChIP-seq narrow peaks were called by MACS2, with parameters “-f BAMPE -B -g 921913538 -q 1e-5”
Assembly: Brassica napus ZS11 （Chen et al., 2021）
Supplementary files format and content: tab-delimited text files , peak file

molecule: genomic DNA

organism: Brassica napus

source_name: silique

growth_protocol: a day/night cycle of 16h/8h and a temperature of 22/18℃

extract_protocol:
Materials of HZ62 were collected and cross-linked using 1% formaldehyde (Sigma, F8775) and quenched with 0.2M glycine (Sigma, G7126) at room temperature. About 1g of samples were used for each library. After grinding into fine powder with liquid nitrogen, cell lysis by 1% SDS (Sigma, AM9822) at 4°C.
The chromatin was fragment by sonication using a Bioruptor (Diagenode) into 200-600 bp. ChIP was performed using H3K4me3 and H3K27me3 antibody (Abclonal, A2357, A2363). Then, the protein-DNA complexes were reversed cross-linking using proteinase K (Invitrogen, AM2546) at 55°C. ChIP-DNA was extracted using phenol:chloroform:isoamyl alcohol (Sigma, P3803). ChIP-DNA libraries were constructed by using NEBNext Ultra II DNA library prep kit for Illumina (New England BioLabs, E7645) according to the manufacturer’s guidelines.

tag: tissue

value: silique

tag: cell type

tag: chip antibody

value: H3K4me3 antibody (Abclonal, A2357)

tag: genotype

value: WT

tag: treatment


title: UPN1_CD34_geneexpression

city: Bethesda

first: Shouguo

last: Gao

state: MD

address: 9000 Rockville Pike

department: NHLBI

country: USA

institute: National Institutes of Health

library_source: transcriptomic

data_processing:
The raw data was processed with cellranger.
Paired-end reads were mapped to the hg19 genome with cellranger for gene-level read counts
Sample demultiplexing, barcode processing, alignment, filtering, UMI counting,and csv files of TCR clonality were performed using the cellranger analysis pipeline.
Paired-end reads were mapped to the hg19 genome with cellranger for gene-level read counts
Data normalization, dimension reduction, and differential expression analysis were performed using R and the Seurat R package. Cells were clustered with PhenoGraph.
T cell receptor and B cell receptor VDJ analysis: Cellranger
VDJ of T cells and B cells were infered with cellranger vdj pipeline to get TCR and BCR contig annotations.
Genome_build: hg19
Supplementary_files_format_and_content: expression_data; VDJ

molecule: total RNA

organism: Homo sapiens

source_name: FACS sorted Linjeage-CD34+ bone marrow

extract_protocol: Libraries of gene expression (BMMNCs and Lineage-CD34+HSPCs) for healthy donors 1-4, and UPNs 6,11,1,10, and 13 were generated using Genomics Chromium Single Cell 3' Solution Kit from 10XGenomics. Libraries of gene expression (BMMNCs and Lineage-CD34+HSPCs) and TCR/BCR sequencing for UPNs 14-17 were generated using Genomics Chromium Single Cell 5' Solution Kit from 10XGenomics. Libraries of gene expression of four human leukemic cell lines (U937, Raji, Jurkat, and THP1) with UBA1 knockdown were generated using Illumina NovaSeq.

tag: Sex

value: Male

tag: age

tag: subject id/status

tag: tissue/cell type

value: FACS sorted Linjeage-CD34+ bone marrow


title: PAPS 5

city: Barcelona

first: Cristina

last: Sole Marce

address: Pg Vall Hebron 119-129

country: Spain

institute: Vall Hebron Institute Research

description:
smallRNA 50nts
SAP5_35468_ACAGTG

data_processing:
FASTQC V0.11.4 was used for raw data quality control
Rsubred R package was used for alignment and counting step of raw counts
Statistical language "R" (R version 3.6.2 (2019-12-12), Copyright The R Foundation for Statistical Computing), and the libraries developed for the miRNAseq analysis in the Bioconductor Projec was used for the rest of steps of the previous pipeline
Homo sapiens GRCh38, realease 86, (downloaded from Ensembl) was used for maping step
Assembly: Homo sapiens GRCh38, realease 86
Supplementary files format and content: tab-delimited text files include RPKM values for each Sample

source_name: sEVs from plasma

extract_protocol:
RNA was harvested using Rneasy mini plus kit (Qiagen)
miRNA libraries for miRNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.

value: sEVs from plasma

tag: subject status


title: Gastrocnemius muscle, WT 3

city: Nagoya

first: Koji

last: Ohashi

state: Aichi

address: 65 Tsurumai-cho, Showa-ku

country: Japan

institute: Nagoya University

data_processing:
Sequence reads were trimmed for adaptor sequence/low-quality sequence using TrimGalore! (ver.0.6.4).
Trimmed sequence reads were mapped to GRCm39 GENCODE vM30 using STAR (2.7.3a).
Read count extraction was performed using HTSeq-count (ver.0.11.2) .
Assembly: GRCm39 GENCODE vM30
Supplementary files format and content: tab-delimited text file includes read count values for each Sample

organism: Mus musculus

source_name: Gastrocnemius muscle

extract_protocol:
RNA was harvested using Rneasy mini kit (Qiagen), and isolated total RNA was used for the construction of sequencing libraries.
RNA libraries for RNA-seq were prepared using TruSeq Stranded mRNA Library Prep Kit following manufacturer's protocols.

value: Gastrocnemius muscle

value: Sciatic nerve denervation


title: HZ62_silique_H3K4me3_BHCS-180

treatment_protocol: a day/night cycle of 16h/8h and a temperature of 22/18℃


title: Gastrocnemius muscle, WT 1


title: PAPS 7

description:
smallRNA 50nts
SAP7_35468_CAGATC


title: Gastrocnemius muscle, WT 2


title: HZ62_silique_H3K27me3_BHCS-182

value: H3K27me3 antibody (Abclonal,  A2363)

value: a day/night cycle of 16h/8h and a temperature of 22/18℃


title: HZ62_silique_H3K27me3_BHCS-199


title: UPN11_CD34_geneexpression


title: PAPS 4

description:
smallRNA 50nts
SAP4_35468_TGACCA


title: HZ62_leaf_H3K4me3_BHCS-054

source_name: seedling

value: seedling

value: 2 weeks


title: SRA1

city: Changchun

first: Ying

last: Zhang

state: Select...

address: 1 Xinmin Street

institute: Department of Pediatric Respiratory, The First Hospital of Jilin University

data_processing:
After the library is qualified, the different libraries are pooling according to the effective concentration and the target amount of data off the machine, then being sequenced by the Illumina NovaSeq 6000. The end reading of 150bp pairing is generated. The basic principle of sequencing is to synthesize and sequence at the same time (Sequencing by Synthesis). Four fluorescent labeled dNTP, DNA polymerase and splice primers were added to the sequenced flowcell and amplified. When the sequence cluster extends the complementary chain, each dNTP labeled by fluorescence can release the corresponding fluorescence. The sequencer captures the fluorescence signal and converts the optical signal into the sequencing peak by computer software, so as to obtain the sequence information of the fragment to be tested.
The image data measured by the high-throughput sequencer are converted into sequence data (reads) by CASAVA base recognition. Raw data (raw reads) of fastq format were firstly processed through fastp software. In this step, clean data (clean reads) were obtained by removing reads containing adapter, reads containing N base and low quality reads from raw data. At the same time, Q20, Q30 and GC content the clean data were calculated. All the downstream analyses were based on the clean data with high quality.
featureCounts (v1.5.0-p3) was used to count the reads numbers mapped to each 2 gene. And then FPKM of each gene was calculated based on the length of the gene and reads count mapped to this gene. FPKM, expected number of Fragments Per Kilobase of transcript sequence per Millions base pairs sequenced, considers the effect of sequencing depth and gene length for the reads count at the same time, and is currently the most commonly used method for estimating gene expression levels.
Differential expression analysis of two conditions/groups (two biological replicates per condition) was performed using the DESeq2 R package (1.20.0). DESeq2 provide statistical routines for determining differential expression in digital gene expression data using a model based on the negative binomial distribution. The resulting P-values were adjusted using the Benjamini and Hochberg’s approach for controlling the false discovery rate . padj<=0.05 and |log2(foldchange)| >= 1 were set as the threshold for significantly differential expression
Assembly: mm10

source_name: lung

extract_protocol:
The total RNA was harvested using Trizol reagent. Illumina NEBNext Ultra Directional RNA Library Prep Kit(NEB) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols

value: lung

value: Atomized with NTHi OMVs and OVA, intraperitoneal injected with OVA


title: SRA2


title: PAPS 8

description:
smallRNA 50nts
SAP8_35468_ACTTGA


title: UPN6_CD34_geneexpression


title: HZ62_leaf_H3K4me3_BHCS-053


title: PAPS 3

description:
smallRNA 50nts
SAP3_35468_TTAGGC


title: HD4_CD34_geneexpression


title: NC3

value: Atomized with  0.9% saline, intraperitoneal injected with PBS


title: HZ62_leaf_H3K27me3_BHCS-066


title: HD2_CD34_geneexpression


title: Control 8

description:
smallRNA 50nts
HD8_35468_GGTAGC

value: Healthy donor


title: PAPS 2

description:
smallRNA 50nts
SAP2_35468_CGATGT

value: PAPS patient


title: NC1


title: HD3_CD34_geneexpression


title: NC2


title: PAPS 1

description:
smallRNA 50nts
SAP1_35468_ATCACG


title: HZ62_leaf_H3K27me3_BHCS-065


title: HZ62_flower_H3K4me3_BHCS-192

source_name: flower bug

value: flower bug

value: <2 mm


title: SRA3


title: K562_Pyruvate_replicate4

city: Haining

first: Mikael

last: Bjorklund

state: Zhejiang

address: 718, Haizhoudong Road, Xiashi Street

institute: Zhejiang University-University of Edinburgh Institute

data_processing:
Sequenced reads were trimmed for adaptor sequences by Novogene
Paired-end reads were mapped to ENSEMBL human transcripts (v100) using Salmon (v1.2.1) using -l A -p 8 --gcBias --validateMappings and parameters.
Raw counts were combined from Salmon output using tximport (v1.14.2) in R (v3.6.3)
DESeq2 (v1.26.0) in R (v3.6.3) is used for differential expression gene calling analysis

growth_protocol: K562 cells were maintained in 5% FBS in RPMI-1640 (with 2mM glutamine and penicillin&streptomycin) in a cell density between 0.2-1x10e6 cells/ml.

extract_protocol:
Cells were collected by centifugation (500xg for 3min), washed with PBS and RNA was harvested using RNA isolater Total RNA Extraction Reagent (Vazyme Biotech, Cat.No.#R401-01). Lysates were stored at -80C and processed by Novogene.
Sequencing libraries were generated from 1ug total RNA using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample. mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer(5X). First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse  Transcriptase(RNase H-). Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3’ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure were ligated to prepare for hybridization. In order to select cDNA fragments of preferentially 250~300 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then 3 μl USER Enzyme (NEB, USA) was used with size-selected, adaptor-ligated cDNA at 37°C for 15 min followed by 5 min at 95 °C before PCR. Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system.

treatment_protocol: Four replicates treated with AKB or pyruvate for 24 hours.

value: chronic myelogenous leukemia

tag: cell line

value: 2 mM Pyruvate (Biological Industries Cat.No. #03-042-1B) for 24h


title: Control 7

description:
smallRNA 50nts
HD7_35468_GAGTGG


title: YN3, scRNA-seq

city: Houston

first: Wen

last: Jiang

state: TX

address: 1515 Holcombe Blvd

institute: MD Anderson Cancer Center

description: 10x Genomics

library_source: transcriptomic single cell

data_processing:
Raw sequencing reads were mapped to the reference genome GRCm39 using the Cell Ranger v.3.1 software (10x Genomics) with default parameters.
Assembly: mm39
Supplementary files format and content: Tab-separated values files and matrix files

source_name: liver

extract_protocol:
Livers were removed from mice after inferior vena cava perfusion. On washing with DMEM, the livers ware cut up in a dish with scissors. Then the livers were then digested into a single cell suspension using liver dissociation kit (Miltenyi, 130-105-807), referring to the script provided by the manufacturer. The cell suspension was filtered through a 70μm strainer and then centrifuged by 300g for 10 minutes at 4℃. Add 70%, 60%, 50%, 40%, 30%, 20% and 10% percoll for 1ml successively to 15ml centrifuge tube, end with the addition of 1ml cell suspension resuspended by DMEM. Next, the tube was centrifuged by 1200g for 15 minutes at 4℃. Extract the layers where 30%, 40% and 50% percoll were located and wash the cells with 1ml DMEM. After counting, 3x10^5 cells were removed and resuspended with 200μl wash buffer (PBS with 2% FBS). The cell samples were blocked by 1μg CD16/32 for 10 minutes at room temperature. Then 2μl CD11b-BV711 antibody was added. The cell suspensions were mixed and incubated for 20 minutes at room temperature, away from light. After being centrifuged by 300g for 10 minutes at 4℃, the cells were washed with wash buffer once and then resuspended with 500μl wash buffer. CD11b-positive cells were then separated by flow cytometer.
Single-cell 3’ gene expression libraries for each of the 12 samples were prepared separately following the protocols of the Chromium Single Cell 3’ Library & Gel Bead kit V3. Briefly, cells and gel beads containing the poly-T primer sequence connected with cell barcode and UMI (unique molecular identifier) were wrapped by "oil droplets" to form GEM (Gel Bead in Emulsion), in which cells lysed and completed reverse transcription according to the manufacturer’s instructions. RNA transcripts from single cells were uniquely barcoded. After reverse transcription, barcoded cDNAs were purified, amplified, end-repaired, and ligated with Illumina adapters as per the manufacturer’s protocol to generate a single multiplexed library. All resulting libraries were sequenced on the Illumina Novaseq 6000 platform.

treatment_protocol: Young and old C57BL6 mice were intravenously injected with 100 nm PEGylated polystyrene nanoparticles at the concentration of 10^11 np/20g of body weight. PBS was injected for control. Mice were euthanized 24 hours after the injection.

value: liver

value: 10 weeks old


title: HZ62_flower_H3K27me3_BHCS-187

value: WT


title: HZ62_flower_H3K4me3_BHCS-174

value: H3K4me3 antibody (Abclonal, A2357)


title: K562_Pyruvate_replicate3


title: YN4, scRNA-seq

description: 10x Genomics


title: K562_Pyruvate_replicate2


title: Control 6

description:
smallRNA 50nts
HD6_35468_CGTACG

value: sEVs from plasma


title: YN2, scRNA-seq

description: 10x Genomics


title: Control 5

description:
smallRNA 50nts
HD5_35468_GTTTCG


title: Control 4

description:
smallRNA 50nts
HD4_35468_GTGGCC


title: HD1_CD34_geneexpression

value: Male

value: FACS sorted Linjeage-CD34+ bone marrow


title: K562_Pyruvate_replicate1


title: Control 3

description:
smallRNA 50nts
HD3_35468_GTGAAA


title: Control 2

description:
smallRNA 50nts
HD2_35468_GTCCGC


title: YC3, scRNA-seq

description: 10x Genomics


title: control_Medulla

city: Hamilton

first: Dan

last: Sturdevant

state: MT

address: 903 S 4th street

department: NIAID

institute: NIH

data_processing:
Illumina Casava1.7 software used for basecalling.
Raw fastq reads were trimmed of Illumina adapter sequences using cutadapt version 1.12
then trimmed and filtered for quality using the FASTX-Toolkit (Hannon Lab)
Remaining reads were aligned to the Homos sapiens
Reads mapping to genes were counted using htseq-count (Anders et al., 2015)
Differential expression analysis was performed using the Bioconductor package DESeq2  (Love et al., 2014)
Assembly: GCF_000001405.38_GRCh38.p12
Supplementary files format and content: normalized_counts.txt are from deseq2 in matrix format

source_name: Medulla

extract_protocol:
RNA was extracted using Qiagen AllPrep
Truseq Stranded mRNA-Seq Sample Preparation Kit (Illumina) was used to synthesize cDNA and generate sequencing ready libraries following the manufacturer's protocol

value: Medulla

value: control


title: K562_AKB_replicate3

value: 2 mM alpha-ketobutyrate (SigmaAldrich Cat.No. #K401) for 24h


title: control_Cortex_2

source_name: Cortex

value: Cortex


title: Control 1

description:
smallRNA 50nts
HD1_35468_GTAGAG

value: Healthy donor


title: control_Thalamus

source_name: Thalamus

value: Thalamus


title: control_Cortex_1


title: K562_AKB_replicate2


title: YC2, scRNA-seq

description: 10x Genomics


title: YC1, scRNA-seq

description: 10x Genomics


title: HZ62_flower_H3K27me3_BHCS-175

value: flower bug

value: H3K27me3 antibody (Abclonal,  A2363)

value: a day/night cycle of 16h/8h and a temperature of 22/18℃


title: YN1, scRNA-seq

description: 10x Genomics


title: Seedlings, Ethylene Rep 2

city: Knoxville

first: Brad

middle: M

last: Binder

state: Tennessee

address: 309 Ken and Blaire Mossman Bldg.

department: Biochemistry & Cellular and Molecular Biology

institute: University of Tennessee

data_processing:
Evaluate sequence quality.
Trim reads.
Map reads to genome of Arabiopsis thaliana.
Generate hit counts for genes/exons.
Compare gene hit counts.
Assembly: Arabidopsis_thaliana_ENSEMBL_ERCC
Supplementary files format and content: CSV formatted files containing differential gene expression data for all genes and for significant DEGs

organism: Arabidopsis thaliana

source_name: Seeds germinated 3 d in dark ethylene

extract_protocol:
Trizol
mRNA enrichement, fragmentation, and random priming. cDNA synthesis, End repair, 5' phosphorylation, and dA-tailing. Adaptor ligation, PCR enrichement, and sequencing.

treatment_protocol: Seeds were germinated in darkness for 3 d in presence or absence of 0.7 ppm ethylene followed by 5 d in light without ethylene.

value: seedlings

value: Columbia

value: Ethylene pretreatment for 3 d, then 5 d in light


title: Seedlings, Ethylene Rep 3


title: K562_CTRL_replicate4

value: chronic myelogenous leukemia

value: untreated


title: Seedlings, Ethylene Rep 1


title: AstV-ND-1_5astro_unknown

source_name: unknown

value: unknown

value: infected with astrovirus


title: control_Cerebellum

source_name: Cerebellum

value: Cerebellum


title: K562_AKB_replicate4


title: ON1, scRNA-seq

description: 10x Genomics

value: liver

value: 15 months old


title: K562_CTRL_replicate3


title: Seedlings, Air Rep 3

source_name: Seeds germinated 3 d in dark no ethylene

value: Air pretreatment for 3 d, then 5 d in light


title: AstV-ND-3_3astro_frontal lobe

source_name: frontal lobe

value: frontal lobe


title: control_Brainstem

source_name: Brainstem

value: Brainstem

value: control


title: K562_CTRL_replicate2


title: OC1, scRNA-seq

description: 10x Genomics


title: AstV-ND-2_1astro_brain stem

source_name: brain stem

value: brain stem


title: K562_AKB_replicate1

value: 2 mM alpha-ketobutyrate (SigmaAldrich Cat.No. #K401) for 24h


title: ON3, scRNA-seq

description: 10x Genomics


title: ON2, scRNA-seq

description: 10x Genomics


title: Strain_2251_LBS-Ca_25˚C_Biol_Rep2

status: Public on Jul 17 2023

city: Madison

first: Jacob

middle: Alan

last: Vander Griend

state: Wisconsin

address: 1550 Linden Drive

department: Medical Microbiology and Immunology

institute: University of Wisconsin - Madison

data_processing:
Raw FASTQ files were first processed using FASTP v0.20.1 for read quality control.
FASTP processed files were mapped using BWA-MEM v0.7.17.
Mapped reads were enumerated by loci using HTSeq v0.13.5
Differential expression analysis was conducted using EdgeR v3.32.1.
Assembly: Vibrio fischeri ES114 Chromosome 1: CP000020.2, Chromosome 2: CP000021.2, pES100 Plasmid: CP000022.1
Supplementary files format and content: Tab delimited file containing the mapped raw sequencing read pairs for each sample produced by HTSeq.
Supplementary files format and content: Tab delimited file containing the TPM values for each gene within the tested samples.

organism: Aliivibrio fischeri

growth_protocol: V. fischeri strains were grown as 500uL colony spots on either LBS or LBS-10 mM Calcium agar plates for 48 hours.

extract_protocol:
Colony material was scrapped from plates and processed using a QIAgen RNeasy PowerBiofilm kit, followed by an additional purification and on-column DNA digestion with the QIAgen Rneasy MinElute kit. For each sample, 500ng of total RNA was used for library construction.
Libraries were constructed using the Illumina Ribo-Zero Plus rRNA Depletion with Stranded Total RNA kit, with an addition of V. fischeri specific rRNA depeletion probes. Library preparation was conducted at the University of Wisconsin - Madison Biotechnology Center Gene Expression Center & DNA Sequencing Facility.

tag: strain

value: ES114 {delta}binK


title: K562_CTRL_replicate1


title: Strain_2251_LBS-Ca_25˚C_Biol_Rep1


title: Seedlings, Air Rep 1

value: seedlings


title: Strain_3259_LBS_25˚C_Biol_Rep3

value: ES114 rscS* {delta}sypG

value: LBS _Media_25C


title: Strain_3259_LBS_25˚C_Biol_Rep2


title: Strain_2251_LBS-Ca_25˚C_Biol_Rep3


title: Strain_2251_LBS_25˚C_Biol_Rep1


title: OC2, scRNA-seq

description: 10x Genomics


title: Strain_1198_LBS_25˚C_Biol_Rep1

value: ES114 rscS*


title: Strain_1100_LBS_25˚C_Biol_Rep3

value: Wild type ES114


title: Strain_2251_LBS_25˚C_Biol_Rep3


title: Strain_1198_LBS_25˚C_Biol_Rep3


title: Strain_1198_LBS_25˚C_Biol_Rep2


title: Strain_2251_LBS_25˚C_Biol_Rep2


title: Strain_1100_LBS_25˚C_Biol_Rep1


title: N_CTRL30

city: Bergen

first: Oystein

last: Eikrem

address: Haukelandsveien 22

department: Department of Mecine

country: Norway

institute: Haukeland University Hospital

data_processing:
Base calling was performed by RTA 1.17.21.3.
FASTQ files were generated using CASAVA 1.8.2 (Illumina, Inc. San Diego, CA, USA).
Transcript expression values were generated by quasi alignment using Salmon (Patro, Duggal, Love, Irizarry, & Kingsford, 2017) and the Ensembl (GRCh38) human transcriptome.
The quasi.gene.count file was subjected to sequencing batch corrections before final analyses.
Genome_build: GRCh38
Supplementary_files_format_and_content: FA_180119_quasi_gene_quant.txt: all gene counts.
hpbergen2019-Fax-qnBC-18k-LT-COUNTS.csv:  sequencing batch corrected data

source_name: distal tubuli

extract_protocol:
RNA was extracted with the High Pure FFPET RNA Isolation Kit (Roche Diagnostics).
RNA libraries were prepared for sequencing using the Illumina Exome (Access) capture protocol.

tag: patient id

tag: sample type

value: LCM of archival kidney biopsy sections

tag: cohort

tag: timepoint

value: single time point

tag: patient id.cohort.tissue compartment. timepoint

tag: final group annotation


title: N_CTRL28


title: N_CTRL29


title: Strain_1100_LBS_25˚C_Biol_Rep2


title: N_CTRL27

source_name: proximal tubuli


title: N_CTRL31


title: Seedlings, Air Rep 2

tag: tissue

value: Columbia


title: N_CTRL25


title: N_CTRL21

source_name: arteries


title: N_CTRL23


title: N_CTRL26


title: N_CTRL24


title: Strain_3259_LBS_25˚C_Biol_Rep1


title: N_CTRL18


title: N_CTRL16


title: N_CTRL15


title: N_CTRL13


title: N_CTRL12


title: N_CTRL17


title: N_CTRL10


title: N_CTRL8

source_name: glomeruli


title: N_CTRL5


title: N_CTRL9


title: N_CTRL7


title: N_CTRL4


title: N_CTRL6


title: N_CTRL3


title: N_CTRL2


title: N_CTRL1


title: N_CTRL19


title: FA131


title: FA128


title: FA129


title: FA125


title: FA127


title: FA126


title: FA124


title: FA121


title: FA123


title: FA120


title: FA118


title: N_CTRL11


title: FA122


title: FA115


title: FA114


title: FA113


title: FA108


title: FA104


title: FA103


title: FA112


title: FA102


title: FA110


title: FA101


title: FA100


title: FA116


title: FA119


title: FA88


title: FA95


title: FA93


title: FA90


title: FA83


title: FA96


title: FA92


title: FA89


title: FA86


title: FA91


title: FA85


title: FA99


title: FA87


title: FA82


title: FA98


title: FA84


title: FA81


title: FA106


title: FA80


title: FA94


title: FA78


title: FA75


title: FA76


title: FA79


title: FA77


title: FA72


title: FA74


title: FA69


title: FA70


title: FA68


title: FA61


title: FA57


title: FA64


title: FA56


title: FA58


title: FA53


title: FA54


title: FA60


title: FA52


title: FA55


title: FA66


title: FA51


title: FA62


title: FA45


title: FA44


title: FA48


title: FA43


title: FA41


title: FA42


title: FA49


title: FA40


title: FA37


title: FA39


title: FA38


title: FA36


title: FA35


title: FA65


title: FA50


title: FA31


title: FA32


title: N_CTRL14


title: FA34


title: FA25


title: FA29


title: FA21


title: FA24


title: FA22


title: FA20


title: FA19


title: FA28


title: FA23


title: FA33


title: FA18


title: FA17


title: FA13


title: FA12


title: FA16


title: FA15


title: FA11


title: FA9


title: FA10


title: FA8


title: FA7


title: FA6


title: FA30


title: FA27


title: FA26


title: FA5


title: FA4


title: FA1


title: 8L_M_3068

city: La Jolla

first: Joseph

middle: R

last: Ecker

state: CA

address: 10010 North Torrey Pines Road

department: Genomic Analysis Laboratory

institute: HHMI-Salk-Institute

data_processing:
Reads trimed with Cutadapt for adapters and low quality bases (Phred score >= 20)
The resulting files are mapped to in-silico bisulfite converted mm10 reference genome using Bismark.
The raw BAM file will be filtered using samtools to filter reads by MAPQ > 10 and use Picard to remove PCR duplicates
Tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls will be generated with in-house developing package using the filtered BAM files
Assembly: mm10
Supplementary files format and content: tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls. Columns are: 1. Chromosome, 2. Position, 3. Strand, 4. Cytosin Context, 5. methylated base call, 6. total mapped base call, 7. not used, place holder column
Library strategy: snmC-seq2

source_name: C57BL/6J Male P56 Mouse Brain

growth_protocol: All mice are C57BL/6J Male, brain dissected in P56

extract_protocol:
the brain of P56 male C57BL/6J mouse will be removed and cut into 600 microns thick coronal sections using a tissue matrix. Then each region will be microdissected from the coronal tissue sections based on identified landmarks and brain atlas coordinates, as defined in Allen brain atlas CCF3 (Avaliable at: http://atlas.brain-map.org). For each dissection region, the tissue from several littermate mice will be combined together into to separate replicate groups. The number of animals in a replicate depends on the size of the tissue. Nuclei preparation will be stained with the fluorescently labeled anti-NeuN body and nucleic acid stain Hoechst 33342.
Library prepared following protocol discribed in Luo, Chongyuan, Angeline Rivkin, Jingtian Zhou, Justin P. Sandoval, Laurie Kurihara, Jacinta Lucero, Rosa Castanon, et al. 2018. “Robust Single-Cell DNA Methylome Profiling with snmC-seq2.” Nature Communications 9 (1): 3824.

tag: brain region

value: VISC


title: 8L_M_3064


title: 8L_M_3066


title: 8L_M_3057


title: 8L_M_3060


title: 8L_M_3058


title: 8L_M_3052


title: 8L_M_3055


title: 8L_M_3056


title: 8L_M_3065


title: 8L_M_3053


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title: 8L_M_3054


title: 8L_M_3046


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title: 8L_M_3044


title: 8L_M_3043


title: FA2


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title: 8K_M_2308


title: 8K_M_2309


title: 8K_M_2304


title: 8K_M_2302


title: 8K_M_2303


title: 8K_M_2301


title: 8K_M_2296


title: 8K_M_2299


title: 8K_M_2292


title: 8K_M_2297


title: 8K_M_2293


title: 8K_M_2298


title: 8K_M_2295


title: 8K_M_2294


title: 8K_M_2291


title: 8K_M_2310


title: 8K_M_2288


title: 8K_M_2285


title: 8K_M_2289


title: 8K_M_2311


title: 8K_M_2287


title: 8K_M_2286


title: 8K_M_2283


title: 8K_M_2282


title: 8K_M_2281


title: 8K_M_2279


title: 8K_M_2305


title: 8K_M_2278


title: 8K_M_2280


title: 8K_M_2300


title: 8K_M_2277


title: 8K_M_2275


title: 8K_M_2276


title: 8K_M_2274


title: 8K_M_2290


title: 8K_M_2272


title: 8K_M_2273


title: 8K_M_2271


title: 8K_M_2268


title: 8K_M_2270


title: 8K_M_2269


title: 8K_M_2267


title: 8K_M_2266


title: 8K_M_2265


title: 8K_M_2264


title: 8K_M_2263


title: 8K_M_2257


title: 8K_M_2260


title: 8K_M_2261


title: 8K_M_2262


title: 8K_M_2259


title: 8K_M_2255


title: 8K_M_2254


title: 8K_M_2252


title: 8K_M_2253


title: 8K_M_2250


title: 8K_M_2284


title: 8K_M_2249