title: Vcap,H3K4me1,ABCAM_1


title: Gal_2

status: Public on Jul 08 2023

type: SRA

city: Harbin

first: Zheng

last: Chen

state: Heilongjiang

address: 92 Xidazhijie  Harbin Institute of Technology

country: China

institute: Harbin Institute of Technology

library_source: transcriptomic

data_processing:
Raw data (raw reads) of fastq format were firstly processed through in-house  perl scripts. In this step, clean data (clean reads) were obtained by removing reads  containing adapter, reads containing ploy-N and low quality reads from raw data. At  the same time, Q20, Q30 and GC content the clean data were calculated. All the  downstream analyses were based on the clean data with high quality.
Reference genome and gene model annotation files were downloaded from  genome website directly. Index of the reference genome was built using Hisat2 v2.0.5  and paired-end clean reads were aligned to the reference genome using Hisat2 v2.0.5. We selected Hisat2 as the mapping tool for that Hisat2 can generate a database  of splice junctions based on the gene model annotation file and thus a better mapping  result than other non-splice mapping tools
featureCounts v1.5.0-p3 was used to count the reads numbers mapped to each  gene. And then FPKM of each gene was calculated based on the length of the gene  and reads count mapped to this gene. FPKM, expected number of Fragments Per  Kilobase of transcript sequence per Millions base pairs sequenced, considers the  effect of sequencing depth and gene length for the reads count at the same time, and is  currently the most commonly used method for estimating gene expression levels.
Differential expression analysis of two  conditions/groups (two biological replicates per condition) was performed using the  DESeq2 R package (1.16.1). DESeq2 provide statistical routines for determining  differential expression in digital gene expression data using a model based on the  negative binomial distribution. The resulting P-values were adjusted using the  Benjamini and Hochberg’s approach for controlling the false discovery rate . Genes  with an adjusted P-value <0.05 found by DESeq2 were assigned as differentially  expressed
Genome_build: GRCm38.p6
Supplementary_files_format_and_content: abundance measurements

molecule: total RNA

organism: Mus musculus

growth_protocol: Primary hepatocytes were isolated from C57BL/6 mice by liver-perfusion with type II collagenase (Worthington Biochem, Lakewood, NJ) and cultured at 37°C and 5% CO2 in RPMI 1640 medium supplemented with 2% FBS.

extract_protocol:
Total RNAs were extracted using TriPure Isolation Reagent (Roche, Mannheim, Germany).
RNA libraries were prepared for sequencing using standard Illumina protocols

treatment_protocol: Primary hepatocytes were infected with βGal and HuR adenoviruses overnight.

tag: tag

tag: genotype/variation

value: primary hepatocytes

value: beta-Gal


title: 22Rv1 input_rep2

city: Chicago

first: Chuan

last: He

state: Illinois

address: 929 E 57th St, Room E319B

country: USA

institute: University of Chicago

data_processing:
Adapter trimming by Cutadapt
Reads mapping by STAR to hg19 transcriptome
Peak calling by omniCLIP
Assembly: hg19
Supplementary files format and content: For [Dataset 1], excel files with peaks annotated by omniCLIP

organism: Homo sapiens

source_name: 22Rv1 cells

growth_protocol: Wild-type 22Rv1 cells are maintained in RPMI1640 + 10% FBS.

extract_protocol:
For [Dataset 1], cell lysates were treated with Rnase T1 to fragment, and RBM15-bound RNAs were extracted by immunoprecipitation. Both input and CLIP RNA were subjected to end-repair with T4 PNK.
For [Dataset 1], Rna libraries were constructed with NEBNext® Small RNA Library Prep Set for Illumina®(NEB, E7330S)

tag: cell type

value: wild-type (ATCC, CRL-2505)

tag: ip antibody


title: Vcap,H3K27ac,ABCAM_2

city: Rochester

first: LIGUO

last: WANG

state: MN

address: 200 1st St SW

department: Division of Computational Biology

institute: Mayo Clinic

library_source: genomic

data_processing:
Basecalls were performed using bcl2fastq v2.17 for Novaseq output.
ChIP-seq reads were trimmed from 3' end until the final base had a quality score > 30, using Trimmomatic v0.33, discarding reads left with < 20 bp
ChIP-seq reads were aligned to the UCSC hg19 genome (GRCh37) using Bowtie version 1.1.2. Only uniquely mapping reads with at most two mismatches were retained.
To create ChIP-seq coverage plots, the locations of the mapped ChIP-seq reads were extended to 150 bp to represent sequenced fragments, renormalized (to reads per million, rpm) and reformatted in the bigWig file format.
Peaks were called uing MACS v2.1.0 with the significance cut-off q-value <=0.01
bigWig files were generated using the genomicRanges R package. Score represents the normalized coverage of DNA fragments at a given genomic coordinate. narrowPeak files were generated using MACS v2 with default settings.
Assembly: hg19/GRCh37
Supplementary files format and content: bigWig, narrowPeak (except for Input sample)

molecule: genomic DNA

source_name: Vcap

growth_protocol: DMEM, 10% fetal bovine serum, 2mM glutamine, 1x penicillin/streptomycin. Cells were cultured at 37C at 5% CO2.

extract_protocol:
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a E220 focused-ultrasonicator (Covaris).
ChIP-seq libraries were prepared using the KAPA HTP Library Preparation Kit complemented with NEXTflex DNA Barcodes from Bioo Scientific. 10 ng of DNA was used as starting material for input and ip samples. Libraries were amplified using 13 cycles on the thermocycler. Post amplification libraries were size selected at 250-450bp in length using Agencourt AMPure XP beads from Beckman Coulter. Libraries were validated using the Agilent High Sensitivity DNA Kit.

tag: cell line

value: Vcap

value: prostate cancer cell line

tag: chip antibody


title: Gal_1


title: 22Rv1 input_rep1


title: LN229 WT sample 1

city: Dallas

first: Lei

last: Guo

state: TX

address: 5323 Harry Hines Blvd

department: Department of Population and Data Sciences

institute: University of Texas Southwestern Medical Center

data_processing:
Raw reads were aligned to hg38 using STAR 2.7.3a
Gene expression was quantified using Subread 1.6.3
Gene expression was normalized using R package DESeq2

molecule: polyA RNA

growth_protocol: All cells were maintained in DMEM supplemented with 5% FBS in humidified incubator with 5% CO2 at 37C.

extract_protocol:
RNA samples were extracted by Genewiz (now Azenta)
Library was constructed by Genewiz (now Azenta)

value: Glioblastoma

tag: genotype

value: WT


title: LN229 WT sample 2


title: Vcap,H3K27ac,ABCAM_1


title: IκB-RNAi-7d

status: Public on Jul 07 2023

city: Beijing

first: Bowen

last: Shuai

state: Beijing

address: Tsinghua University, Haidian District, Beijing

department: School of Life Science

institute: Tsinghua university

data_processing:
Data filtering with software SOAPnuke
Alignment of clean reads to reference genome sequences using HISAT
Alignment of clean reads to reference gene sequences using Bowtie2
Calculation of gene and transcript expression levels using RSEM
Assembly: pfu_genome2.0 (can be downloaded from the website: https://marinegenomics.oist.jp/pearl/viewer/download?project_id=36)
Supplementary files format and content: tab-delimited text file includes FPKM values for each sample

organism: Pinctada fucata

source_name: mantle

growth_protocol: Pearl oysters Pinctada fucata

extract_protocol:
A piece of the mantle tissue with similar size was collected from each of the 5 oysters in one group. We extracted total RNA from specific tissues according to the instructions provided with TRIZOL reagent (Invitrogen).
1) Total RNA was treated using mRNA enrichment or rRNA depletion methods. For mRNA enrichment, magnetic beads with oligo(dT) were used to capture mRNA with poly(A) tails. For rRNA depletion, DNA probes were hybridized to rRNA, and RNase H selectively digested the DNA/RNA hybrids. After digestion of the DNA probes using DNase I, the desired RNA was obtained through purification.  2) The obtained RNA fragments were fragmented using a disruption buffer. Reverse transcription was performed using random hexamers (N6 primers) to synthesize double-stranded cDNA.  3) The synthesized double-stranded DNA was end-repaired and 5'-phosphorylated. The 3' ends were extended by adding a single "A" overhang and then ligated with a bubble-shaped adapter with a 3' protruding "T".  4) The ligation product was subjected to PCR amplification using specific primers.  5) The PCR product was denatured to form single-stranded DNA and subsequently circularized into single-stranded circular DNA libraries using a bridging primer.

treatment_protocol: Double-stranded RNAs (dsRNAs) were synthesized according to the instructions of the RiboMAXTM Large Scale RNA Production System T7 Kit (Promega, USA). Healthy adult oysters with similar size and weight were injected with 30 μg different dsRNAs into the adductor muscle, and each treatment group included five oysters.

tag: tissue

value: mantle

tag: treatment

value: poI-kappaB dsRNA injection


title: Rel-RNAi-7d

value: Pf-Rel dsRNA injection


title: control

value: GFP dsRNA injection


title: Vcap,Input


title: HuR_1

source_name: HuR-overexpressing primary hepatocytes

value: HuR


title: Patient number 190, Sample Collection Timepoint 1

type: RNA

city: Memphis

first: Jeremy

middle: Chase

last: Crawford

state: TN

address: MS 221, 262 Danny Thomas Place

department: Department of Host-Microbe Interactions

institute: St. Jude Children's Research Hospital

hyb_protocol: Reporter and Capture ProbeSets were manufactured within the same lot to reduce variability. Incorporated into these reagents were the NanoString manufactured 6 positive controls and 8 negative controls so that each sample reported control values. To prepare the RNA for the NanoString run, each sample was diluted to 17 ng/µl in RNase-free water (Qiagen) and 5 µl added into small PCR tubes with 8 µl of a mastermix (containing 3 ul Reporter CodeSet and 5 µl hybridization buffer) followed by 2 µl of Capture ProbeSet. After capping the tubes to mix by inversion, they were pulse centrifuged and incubated for16 hours overnight at 65°C (70°C heated lid) on a thermal cycler (details) then stabilized at 4°C and run within 24 hours. To obtain a loading volume of 30 µl, 15 µl of RNase-free water was added to each sample.

scan_protocol: Samples were prepared using Reporter and Capture ProbeSets and then loaded individually onto the NanoString cartridge. Non-amplified mRNA was quantified using the nCounter® SPRINT Profiler (NanoString Technologies, Inc., Seattle, WA) in batches of 12.

data_processing: NanoString data were assessed for common quality control measures using the NACHO R package. Seven a priori defined housekeeping genes were assessed for frequency and degree of expression, and five of those were subsequently used to normalize the data with the geometric mean method (B2M, DECR1, HPRT1, POLG, PPIB).

label: NanoString

source_name: blood

extract_protocol: For RNA extraction, the PAXgene Blood RNA Kit (Qiagen, Waltham, MA) was used with the QIAcube instrument as per manufacturer’s guidelines with minor modifications based on blood volume and white blood count (WBC). If WBC count (x106 WBC/ml) was obtained close to the blood collection time, it was multiplied by the volume of blood (ml) to estimate the total number of cells. If the estimated total WBC count was between 12x106 - 27.5x106, then the original protocol was followed as per instructions with 80µl final elution. If the total WBC count was <12x106, then BR5 buffer was omitted from QIAcube setup; instead, after the run was complete, 40 µl of BR5 buffer was added manually to the spin column and centrifuged for 1 min at top speed to elute RNA. The RNA sample was then placed back into the QIAcube to incubate at 65oC for 5 min as per instructions. If the WBC count was ≥27.5x106, the sample was split evenly into two tubes after the second centrifugation step by resuspending the pellet with 700 µl of BR1 (instead of 350 µl). The QIAcube was set up to use two separate spin columns. If no WBC count was available, the resuspended cell pellet was assessed for opacity (increased opacity = increased cellular material) to determine whether to split the sample or follow the original protocol. All RNA extracts were tested for quality and quantity using the Agilent 2100 Bioanalyzer and Agilent RNA 6000 Nano Kit (Agilent Technologies, Inc., Santa Clara, CA) per the manufacturer’s instructions. RNA samples were then aliquoted and stored at -80oC until use. The PAXgene Blood RNA Kit incorporated an on-column DNase digestion, with additional DNase treatment applied as needed following quality assessment using the DNA-free™ kit (Ambion, Life Technologies, Carlsbad, CA). RNA analysis was again validated on the Bioanalyzer before use.

value: blood

tag: patient number

tag: patient had a second sample?

value: No

tag: sample collection time point

tag: longitudinal data

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

tag: final mods category based on manuscript definitions

value: Never MODS

tag: age

value: 17.6 years

tag: Sex

value: Male

tag: previously healthy

value: Yes

tag: influenza virus result

value: Influenza B

tag: other viral co-infection

tag: bacterial co-infection

value: MRSA or MSSA

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: ali/ards

tag: survived?

tag: abs_neutrophil_count (10^3/ul)

tag: psofa score on day of sample 1

tag: psofa score on day of sample 2


title: Patient number 191, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

tag: longitudinal data

value: 17.9 years

value: Influenza A - No Subtype


title: Patient number 188, Sample Collection Timepoint 1

tag: final mods category based on manuscript definitions

tag: age

value: 16.4 years

tag: Sex

value: Female

tag: previously healthy

tag: influenza virus result

value: Influenza A H3

value: ARDS


title: Patient number 187, Sample Collection Timepoint 1

value: 15.4 years

value: Influenza A H1 Pandemic

tag: other viral co-infection

tag: bacterial co-infection

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support


title: Patient number 185, Sample Collection Timepoint 1

value: blood

value: 15.3 years

value: Other

tag: total hours in icu

tag: ali/ards

tag: survived?

tag: abs_neutrophil_count (10^3/ul)

tag: psofa score on day of sample 1


title: Patient number 189, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

value: No

tag: sample collection time point

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

value: Never MODS

value: 17.2 years

tag: psofa score on day of sample 2


title: Patient number 184, Sample Collection Timepoint 1

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

tag: Sex

tag: previously healthy

value: Yes


title: Patient number 183, Sample Collection Timepoint 1

value: 15 years

tag: influenza virus result

tag: other viral co-infection

tag: bacterial co-infection

tag: shock requiring vasopressors

tag: invasive mechanical ventilation


title: Patient number 186, Sample Collection Timepoint 1

tag: ecmo support

tag: total hours in icu

tag: ali/ards

tag: survived?

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 182, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: 14.8 years

value: MRSA or MSSA

tag: psofa score on day of sample 1


title: Patient number 176, Sample Collection Timepoint 1

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

value: 12.5 years

tag: Sex

value: Male

value: Influenza A - No Subtype

tag: psofa score on day of sample 2


title: Patient number 181, Sample Collection Timepoint 1

value: 14.6 years

tag: previously healthy

tag: influenza virus result

value: Influenza A H3

tag: other viral co-infection

tag: bacterial co-infection

tag: shock requiring vasopressors

value: ARDS


title: Patient number 180, Sample Collection Timepoint 1

value: blood

value: 13.9 years

value: Female

value: Influenza A H1 Pandemic

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: ali/ards

tag: survived?


title: Patient number 177, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

value: No

tag: sample collection time point

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

value: 12.6 years

value: Influenza B

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 175, Sample Collection Timepoint 1

tag: longitudinal data

tag: final mods category based on manuscript definitions

value: Never MODS

tag: age

value: 12.2 years

tag: Sex

value: Yes

tag: psofa score on day of sample 1


title: Patient number 178, Sample Collection Timepoint 1

value: 13.2 years

tag: previously healthy

tag: influenza virus result

tag: other viral co-infection

tag: bacterial co-infection

tag: psofa score on day of sample 2


title: Patient number 179, Sample Collection Timepoint 1

value: 13.3 years

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: ali/ards


title: Patient number 174, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: 12 years

tag: survived?


title: Patient number 169, Sample Collection Timepoint 1

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

value: 11.1 years

tag: Sex

value: Male

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 173, Sample Collection Timepoint 1

value: 11.7 years

tag: previously healthy

tag: influenza virus result

tag: other viral co-infection

tag: bacterial co-infection

tag: psofa score on day of sample 1


title: Patient number 168, Sample Collection Timepoint 1

value: blood

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: psofa score on day of sample 2


title: Patient number 167, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: 10.9 years

tag: ali/ards


title: Patient number 172, Sample Collection Timepoint 1

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

value: 11.4 years

tag: Sex

tag: survived?


title: Patient number 164, Sample Collection Timepoint 1

value: 10.4 years

tag: previously healthy

tag: influenza virus result

value: Influenza A H3

tag: other viral co-infection

tag: bacterial co-infection

value: ARDS

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 171, Sample Collection Timepoint 1

value: No

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

value: 11.3 years

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: psofa score on day of sample 1


title: Patient number 166, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: Never MODS

value: Female

tag: psofa score on day of sample 2


title: Patient number 165, Sample Collection Timepoint 1

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

value: 10.7 years

tag: Sex

value: Influenza A - No Subtype

value: No testing

tag: ali/ards

value: Yes


title: Patient number 170, Sample Collection Timepoint 1

value: Male

tag: previously healthy

tag: influenza virus result

tag: other viral co-infection

tag: bacterial co-infection

tag: survived?


title: Patient number 163, Sample Collection Timepoint 1

value: Influenza B

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 162, Sample Collection Timepoint 1

tag: tissue

value: blood

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: 10.1 years

tag: psofa score on day of sample 1


title: Patient number 161, Sample Collection Timepoint 1

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

value: 10 years

tag: Sex

tag: psofa score on day of sample 2


title: Patient number 160, Sample Collection Timepoint 1

value: 9.9 years

tag: previously healthy

tag: influenza virus result

value: Influenza A H1 Pandemic

tag: other viral co-infection

tag: bacterial co-infection

tag: ali/ards


title: Patient number 159, Sample Collection Timepoint 1

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: survived?


title: Patient number 158, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: 9.8 years

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 155, Sample Collection Timepoint 1

value: No

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

value: 9.2 years

tag: Sex

tag: psofa score on day of sample 1


title: Patient number 154, Sample Collection Timepoint 1

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

value: 9 years

tag: previously healthy

tag: influenza virus result

tag: other viral co-infection

tag: bacterial co-infection

tag: psofa score on day of sample 2


title: Patient number 157, Sample Collection Timepoint 1

value: Never MODS

value: 9.5 years

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: ali/ards


title: Patient number 153, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: 8.8 years

value: Female

tag: survived?


title: Patient number 156, Sample Collection Timepoint 1

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

value: 9.4 years

tag: Sex

value: Influenza A H3

value: Other

value: Yes

value: ARDS

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 150, Sample Collection Timepoint 1

value: blood

value: 8.4 years

value: Male

tag: previously healthy

tag: influenza virus result

tag: other viral co-infection

tag: bacterial co-infection

tag: psofa score on day of sample 1


title: Patient number 149, Sample Collection Timepoint 1

value: Influenza A H1 Pandemic

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: psofa score on day of sample 2


title: Patient number 147, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

value: No

tag: sample collection time point

value: 8.2 years

tag: ali/ards


title: Patient number 148, Sample Collection Timepoint 1

tag: longitudinal data

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

tag: final mods category based on manuscript definitions

tag: age

tag: Sex

value: MRSA or MSSA

tag: survived?


title: Patient number 146, Sample Collection Timepoint 1

value: Never MODS

tag: previously healthy

tag: influenza virus result

tag: other viral co-infection

tag: bacterial co-infection

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 140, Sample Collection Timepoint 1

value: 7.3 years

value: Grp A Strep or Pneumococcus

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: psofa score on day of sample 1


title: Patient number 142, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: Influenza A - No Subtype

tag: psofa score on day of sample 2


title: Patient number 143, Sample Collection Timepoint 1

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

value: 7.5 years

tag: Sex

tag: ali/ards


title: Patient number 144, Sample Collection Timepoint 1

value: Female

tag: previously healthy

tag: influenza virus result

tag: other viral co-infection

tag: bacterial co-infection

tag: survived?


title: Patient number 139, Sample Collection Timepoint 1

value: 6.9 years

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

value: ALI

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 134, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: 6.1 years

value: Yes

tag: psofa score on day of sample 1


title: Patient number 137, Sample Collection Timepoint 1

value: blood

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

value: 6.2 years

tag: Sex

tag: psofa score on day of sample 2


title: Patient number 136, Sample Collection Timepoint 1

tag: previously healthy

tag: influenza virus result

value: Influenza A H1 Pandemic

tag: other viral co-infection

tag: bacterial co-infection

tag: ali/ards


title: Patient number 135, Sample Collection Timepoint 1

value: No

value: Male

value: Influenza A H3

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: survived?


title: Patient number 152, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

value: 8.7 years

value: Influenza B

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 151, Sample Collection Timepoint 1

tag: longitudinal data

tag: final mods category based on manuscript definitions

value: Never MODS

tag: age

value: 8.6 years

tag: Sex

tag: psofa score on day of sample 1


title: Patient number 145, Sample Collection Timepoint 1

value: 7.8 years

tag: previously healthy

tag: influenza virus result

tag: other viral co-infection

tag: bacterial co-infection

tag: psofa score on day of sample 2


title: Patient number 133, Sample Collection Timepoint 1

value: 5.9 years

value: MRSA or MSSA

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: ali/ards


title: Patient number 132, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: 5.7 years

tag: survived?


title: Patient number 129, Sample Collection Timepoint 1

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

value: 5.5 years

tag: Sex

value: Female

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 126, Sample Collection Timepoint 1

value: blood

value: 5.1 years

tag: previously healthy

value: Yes

tag: influenza virus result

tag: other viral co-infection

tag: bacterial co-infection

value: ALI

tag: psofa score on day of sample 1


title: Patient number 131, Sample Collection Timepoint 1

value: No

value: 5.6 years

value: Influenza A H1 Pandemic

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: psofa score on day of sample 2


title: Patient number 141, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

value: 7.3 years

value: Influenza A - No Subtype

tag: ali/ards


title: Patient number 128, Sample Collection Timepoint 1

tag: longitudinal data

tag: final mods category based on manuscript definitions

value: Never MODS

tag: age

tag: Sex

value: Male

tag: survived?


title: Patient number 127, Sample Collection Timepoint 1

value: 5.2 years

tag: previously healthy

tag: influenza virus result

value: Influenza A H3

tag: other viral co-infection

tag: bacterial co-infection

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 125, Sample Collection Timepoint 1

value: 4.9 years

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: psofa score on day of sample 1


title: Patient number 124, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: Other

tag: psofa score on day of sample 2


title: Patient number 119, Sample Collection Timepoint 1

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

value: 4 years

tag: Sex

tag: ali/ards


title: Patient number 120, Sample Collection Timepoint 1

value: blood

value: 4.1 years

tag: previously healthy

tag: influenza virus result

tag: other viral co-infection

value: No testing

tag: bacterial co-infection

value: ARDS

tag: survived?


title: Patient number 123, Sample Collection Timepoint 1

value: No

value: 4.6 years

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

value: Yes

tag: ecmo support

tag: total hours in icu

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 138, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

value: 6.5 years

value: Female

tag: psofa score on day of sample 1


title: Patient number 121, Sample Collection Timepoint 1

tag: longitudinal data

tag: final mods category based on manuscript definitions

value: Never MODS

tag: age

value: 4.2 years

tag: Sex

value: MRSA or MSSA

tag: psofa score on day of sample 2


title: Patient number 118, Sample Collection Timepoint 1

value: Male

tag: previously healthy

tag: influenza virus result

value: Influenza A - No Subtype

tag: other viral co-infection

tag: bacterial co-infection

tag: ali/ards


title: Patient number 117, Sample Collection Timepoint 1

value: 3.8 years

value: Influenza A H3

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: survived?


title: Patient number 116, Sample Collection Timepoint 1

tag: tissue

value: blood

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 114, Sample Collection Timepoint 1

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

value: 3.5 years

tag: Sex

value: Grp A Strep or Pneumococcus

tag: psofa score on day of sample 1


title: Patient number 115, Sample Collection Timepoint 1

value: 3.6 years

tag: previously healthy

tag: influenza virus result

value: Influenza B

tag: other viral co-infection

value: Other

tag: bacterial co-infection

tag: psofa score on day of sample 2


title: Patient number 113, Sample Collection Timepoint 1

value: No

value: 3.4 years

value: Yes

value: Influenza A H1 Pandemic

value: No testing

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: ali/ards


title: Patient number 112, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

value: Never MODS

value: 3.3 years

tag: survived?


title: Patient number 130, Sample Collection Timepoint 1

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

value: 5.5 years

tag: Sex

value: Female

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 111, Sample Collection Timepoint 1

value: Male

tag: previously healthy

tag: influenza virus result

value: Influenza A - No Subtype

tag: other viral co-infection

tag: bacterial co-infection

value: ARDS

tag: psofa score on day of sample 1


title: Patient number 110, Sample Collection Timepoint 1

value: blood

value: 3 years

value: Influenza A H3

value: RSV

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: psofa score on day of sample 2


title: Patient number 107, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

value: No

tag: sample collection time point

value: 2.6 years

value: MRSA or MSSA

tag: ali/ards


title: Patient number 108, Sample Collection Timepoint 1

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

tag: Sex

value: Yes

value: Influenza A H1 Pandemic

tag: survived?


title: Patient number 109, Sample Collection Timepoint 1

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

value: 2.9 years

tag: previously healthy

tag: influenza virus result

tag: other viral co-infection

tag: bacterial co-infection

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 122, Sample Collection Timepoint 1

value: Never MODS

value: 4.3 years

value: Other

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: psofa score on day of sample 1


title: Patient number 106, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: 2.4 years

value: Female

value: Influenza A H3/Influenza B

tag: psofa score on day of sample 2


title: Patient number 105, Sample Collection Timepoint 1

value: blood

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

value: 2.2 years

tag: Sex

value: Influenza A - No Subtype

tag: ali/ards


title: Patient number 102, Sample Collection Timepoint 1

value: 2 years

value: Male

tag: previously healthy

tag: influenza virus result

tag: other viral co-infection

tag: bacterial co-infection

tag: survived?


title: Patient number 99, Sample Collection Timepoint 1

value: No

value: 1.8 years

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 104, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: Yes

value: ALI

tag: psofa score on day of sample 1


title: Patient number 101, Sample Collection Timepoint 1

tag: longitudinal data

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

tag: final mods category based on manuscript definitions

tag: age

tag: Sex

value: Influenza A H1 Pandemic

value: Grp A Strep or Pneumococcus

tag: psofa score on day of sample 2


title: Patient number 100, Sample Collection Timepoint 1

value: Never MODS

tag: previously healthy

tag: influenza virus result

value: Influenza B

tag: other viral co-infection

value: RSV

tag: bacterial co-infection

tag: ali/ards


title: Patient number 97, Sample Collection Timepoint 1

value: 1.7 years

value: Female

value: MRSA or MSSA

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: survived?


title: Patient number 95, Sample Collection Timepoint 1

tag: tissue

value: blood

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: 1.4 years

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 87, Sample Collection Timepoint 1

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

value: 0.9 years

tag: Sex

value: Influenza A - No Subtype

tag: psofa score on day of sample 1


title: Patient number 86, Sample Collection Timepoint 1

value: No

value: Male

tag: previously healthy

tag: influenza virus result

tag: other viral co-infection

value: Other

tag: bacterial co-infection

value: ARDS

tag: psofa score on day of sample 2


title: Patient number 85, Sample Collection Timepoint 1

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

value: 0.8 years

value: Influenza A H1 Pandemic/Influenza B

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

value: Yes

tag: ecmo support

tag: total hours in icu

tag: ali/ards


title: Patient number 92, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: Never MODS

value: 1.2 years

value: Influenza A H3

tag: survived?


title: Patient number 88, Sample Collection Timepoint 1

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

value: 1 years

tag: Sex

value: Female

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 94, Sample Collection Timepoint 1

value: blood

value: 1.3 years

tag: previously healthy

tag: influenza virus result

value: Influenza B

tag: other viral co-infection

tag: bacterial co-infection

tag: psofa score on day of sample 1


title: Patient number 83, Sample Collection Timepoint 1

value: Influenza A - No Subtype

value: RSV

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: psofa score on day of sample 2


title: Patient number 82, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

value: No

tag: sample collection time point

value: Male

value: Influenza A H1 Pandemic

tag: ali/ards


title: Patient number 89, Sample Collection Timepoint 1

tag: longitudinal data

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

tag: final mods category based on manuscript definitions

tag: age

value: 1.1 years

tag: Sex

tag: survived?


title: Patient number 103, Sample Collection Timepoint 1

value: 2.2 years

tag: previously healthy

tag: influenza virus result

tag: other viral co-infection

tag: bacterial co-infection

value: MRSA or MSSA

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 93, Sample Collection Timepoint 1

value: Never MODS

value: Yes

value: Influenza A/B

value: Other

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: psofa score on day of sample 1


title: Patient number 80, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: 0.7 years

value: No testing

tag: psofa score on day of sample 2


title: Patient number 98, Sample Collection Timepoint 1

value: blood

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

value: 1.7 years

tag: Sex

value: Female

tag: ali/ards


title: Patient number 91, Sample Collection Timepoint 1

value: 1.2 years

tag: previously healthy

tag: influenza virus result

value: Influenza A H3

tag: other viral co-infection

tag: bacterial co-infection

value: Grp A Strep or Pneumococcus

tag: survived?


title: Patient number 79, Sample Collection Timepoint 1

value: No

value: Male

value: Influenza A H1 Pandemic

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 78, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

value: 0.5 years

value: Influenza A - No Subtype

value: RSV

tag: psofa score on day of sample 1


title: Patient number 96, Sample Collection Timepoint 1

tag: longitudinal data

tag: final mods category based on manuscript definitions

value: Never MODS

tag: age

value: 1.4 years

tag: Sex

value: Yes

tag: psofa score on day of sample 2


title: Patient number 77, Sample Collection Timepoint 1

value: 0.4 years

tag: previously healthy

tag: influenza virus result

value: Influenza B

tag: other viral co-infection

value: Other

tag: bacterial co-infection

tag: ali/ards


title: Patient number 90, Sample Collection Timepoint 1

value: blood

value: 1.1 years

value: Female

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: survived?


title: Patient number 76, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: 0.3 years

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 74, Sample Collection Timepoint 1

value: No

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

tag: Sex

value: ALI

tag: psofa score on day of sample 1


title: Patient number 70, Sample Collection Timepoint 1

value: 0.1 years

value: Male

tag: previously healthy

tag: influenza virus result

tag: other viral co-infection

tag: bacterial co-infection

value: ARDS

tag: psofa score on day of sample 2


title: Patient number 71, Sample Collection Timepoint 1

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

value: 0.2 years

value: Influenza A H3

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: ali/ards


title: Patient number 72, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: Never MODS

value: Influenza A - No Subtype

value: RSV

tag: survived?


title: Patient number 73, Sample Collection Timepoint 1

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

tag: Sex

value: Yes

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 75, Sample Collection Timepoint 1

tag: previously healthy

tag: influenza virus result

tag: other viral co-infection

tag: bacterial co-infection

tag: psofa score on day of sample 1


title: Patient number 84, Sample Collection Timepoint 1

value: 0.8 years

value: Other

value: MRSA or MSSA

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: psofa score on day of sample 2


title: Patient number 81, Sample Collection Timepoint 1

tag: tissue

value: blood

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: 0.7 years

value: Female

value: Influenza A H1 Pandemic

tag: ali/ards


title: Patient number 63, Sample Collection Timepoint 1

value: No

tag: longitudinal data

tag: final mods category based on manuscript definitions

value: Recovered MODS

tag: age

value: 12.5 years

tag: Sex

value: Influenza B

tag: survived?


title: Patient number 64, Sample Collection Timepoint 1

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

value: 16.1 years

tag: previously healthy

tag: influenza virus result

tag: other viral co-infection

tag: bacterial co-infection

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 68, Sample Collection Timepoint 1

value: Never MODS

value: 0.1 years

value: Influenza A/B

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: psofa score on day of sample 1


title: Patient number 69, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: Yes

tag: psofa score on day of sample 2


title: Patient number 66, Sample Collection Timepoint 1

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

tag: Sex

value: Male

value: Influenza A H3

value: RSV

tag: ali/ards

value: ARDS


title: Patient number 67, Sample Collection Timepoint 1

value: blood

tag: previously healthy

tag: influenza virus result

tag: other viral co-infection

tag: bacterial co-infection

value: Other

tag: survived?


title: Patient number 62, Sample Collection Timepoint 1

value: 10.6 years

value: Influenza A H1 Pandemic

value: MRSA or MSSA

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

value: ALI

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 65, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

value: No

tag: sample collection time point

value: Recovered MODS

value: 17.9 years

value: Female

tag: psofa score on day of sample 1


title: Patient number 60, Sample Collection Timepoint 1

tag: longitudinal data

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

tag: final mods category based on manuscript definitions

tag: age

value: 9.2 years

tag: Sex

value: Influenza B

tag: psofa score on day of sample 2


title: Patient number 58, Sample Collection Timepoint 1

value: 5.8 years

value: Male

tag: previously healthy

value: Yes

tag: influenza virus result

tag: other viral co-infection

tag: bacterial co-infection

tag: ali/ards


title: Patient number 61, Sample Collection Timepoint 1

value: 10.3 years

value: Influenza A H3

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

value: ARDS

tag: survived?


title: Patient number 59, Sample Collection Timepoint 1

tag: tissue

value: blood

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: 7.1 years

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 57, Sample Collection Timepoint 1

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

value: 4.8 years

tag: Sex

value: Influenza A - No Subtype

value: Other

tag: psofa score on day of sample 1


title: Patient number 56, Sample Collection Timepoint 1

value: No

value: Recovered MODS

value: 4 years

tag: previously healthy

tag: influenza virus result

value: Influenza A H1 Pandemic

tag: other viral co-infection

tag: bacterial co-infection

tag: psofa score on day of sample 2


title: Patient number 55, Sample Collection Timepoint 1

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

value: 2.8 years

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: ali/ards


title: Patient number 51, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: Prolonged MODS/Died

value: 14.8 years

value: Influenza B

value: MRSA or MSSA

tag: survived?


title: Patient number 54, Sample Collection Timepoint 1

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

value: 2.1 years

tag: Sex

value: Male

value: Yes

value: Influenza A H3

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 53, Sample Collection Timepoint 1

value: blood

value: 16.9 years

tag: previously healthy

tag: influenza virus result

tag: other viral co-infection

tag: bacterial co-infection

value: ARDS

tag: psofa score on day of sample 1


title: Patient number 50, Sample Collection Timepoint 1

value: No

value: 12.8 years

value: Female

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: psofa score on day of sample 2


title: Patient number 52, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: 15.2 years

tag: ali/ards


title: Patient number 49, Sample Collection Timepoint 1

tag: longitudinal data

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

tag: final mods category based on manuscript definitions

tag: age

value: 10 years

tag: Sex

tag: survived?


title: Patient number 44, Sample Collection Timepoint 1

value: Recovered MODS

value: 3.3 years

tag: previously healthy

tag: influenza virus result

value: Influenza A H1 Pandemic

tag: other viral co-infection

tag: bacterial co-infection

value: Grp A Strep or Pneumococcus

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 45, Sample Collection Timepoint 1

value: 14.1 years

value: MRSA or MSSA

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: psofa score on day of sample 1


title: Patient number 48, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: Prolonged MODS/Died

value: 6.1 years

value: Yes

value: Influenza A - No Subtype

value: Other

tag: psofa score on day of sample 2


title: Patient number 46, Sample Collection Timepoint 1

value: blood

value: No

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

value: 0.1 years

tag: Sex

tag: ali/ards

value: ARDS


title: Patient number 47, Sample Collection Timepoint 1

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

value: 4.9 years

value: Female

tag: previously healthy

tag: influenza virus result

value: Influenza B

tag: other viral co-infection

tag: bacterial co-infection

tag: survived?


title: Patient number 45, Sample Collection Timepoint 2

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:Recovered (by the time second sample collected)

value: Male

value: Influenza A H1 Pandemic

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 43, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: Recovered MODS

value: 0.3 years

tag: psofa score on day of sample 1


title: Patient number 44, Sample Collection Timepoint 2

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

value: 3.3 years

tag: Sex

value: Grp A Strep or Pneumococcus

tag: psofa score on day of sample 2


title: Patient number 43, Sample Collection Timepoint 2

value: Yes

tag: previously healthy

tag: influenza virus result

tag: other viral co-infection

tag: bacterial co-infection

tag: ali/ards


title: Patient number 42, Sample Collection Timepoint 2

value: 1.5 years

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: survived?


title: Patient number 41, Sample Collection Timepoint 2

tag: tissue

value: blood

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: 7 years

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 42, Sample Collection Timepoint 1

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

tag: Sex

value: No

tag: psofa score on day of sample 1


title: Patient number 40, Sample Collection Timepoint 1

value: 0.1 years

tag: previously healthy

tag: influenza virus result

value: Influenza A - No Subtype

tag: other viral co-infection

value: RSV

tag: bacterial co-infection

value: ALI

tag: psofa score on day of sample 2


title: Patient number 38, Sample Collection Timepoint 1

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

value: 1.8 years

value: Female

value: Influenza B

value: Other

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: ali/ards

value: ARDS


title: Patient number 39, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: Recovered MODS

value: 8.4 years

value: Male

value: Influenza A H1 Pandemic

tag: survived?


title: Patient number 38, Sample Collection Timepoint 2

value: blood

value: Yes

tag: longitudinal data

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:Recovered (by the time second sample collected)

tag: final mods category based on manuscript definitions

tag: age

tag: Sex

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 37, Sample Collection Timepoint 1

value: 13.4 years

tag: previously healthy

tag: influenza virus result

tag: other viral co-infection

tag: bacterial co-infection

value: No

tag: psofa score on day of sample 1


title: Patient number 39, Sample Collection Timepoint 2

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: psofa score on day of sample 2


title: Patient number 36, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: Prolonged MODS/Died

value: 3.7 years

value: No testing

value: Grp A Strep or Pneumococcus

tag: ali/ards


title: Patient number 36, Sample Collection Timepoint 2

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

tag: Sex

tag: survived?


title: Patient number 35, Sample Collection Timepoint 2

value: 1.9 years

tag: previously healthy

tag: influenza virus result

value: Influenza A H3

tag: other viral co-infection

tag: bacterial co-infection

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 40, Sample Collection Timepoint 2

value: 0.1 years

value: Influenza A - No Subtype

value: RSV

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

value: ALI

tag: psofa score on day of sample 1


title: Patient number 41, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

value: Recovered MODS

value: 7 years

value: Male

value: ARDS

tag: psofa score on day of sample 2


title: Patient number 33, Sample Collection Timepoint 2

value: blood

value: Yes

tag: longitudinal data

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:Recovered (by the time second sample collected)

tag: final mods category based on manuscript definitions

tag: age

value: 13.9 years

tag: Sex

value: Influenza B

value: MRSA or MSSA

tag: ali/ards


title: Patient number 34, Sample Collection Timepoint 1

value: Prolonged MODS/Died

value: 8.1 years

value: Female

tag: previously healthy

tag: influenza virus result

tag: other viral co-infection

tag: bacterial co-infection

value: No

tag: survived?


title: Patient number 32, Sample Collection Timepoint 2

value: 8.2 years

value: Influenza A H1 Pandemic

value: Grp A Strep or Pneumococcus

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 30, Sample Collection Timepoint 2

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: 1.8 years

value: Other

tag: psofa score on day of sample 1


title: Patient number 31, Sample Collection Timepoint 2

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

value: 5.4 years

tag: Sex

value: Influenza A - No Subtype

tag: psofa score on day of sample 2


title: Patient number 35, Sample Collection Timepoint 1

value: blood

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

value: Recovered MODS

value: 1.9 years

value: Male

tag: previously healthy

tag: influenza virus result

value: Influenza A H3

tag: other viral co-infection

tag: bacterial co-infection

tag: ali/ards


title: Patient number 31, Sample Collection Timepoint 1

value: Yes

value: MRSA or MSSA

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

value: ARDS

tag: survived?


title: Patient number 30, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: Prolonged MODS/Died

value: Female

value: No

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 37, Sample Collection Timepoint 2

tag: longitudinal data

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:Recovered (by the time second sample collected)

tag: final mods category based on manuscript definitions

tag: age

value: 13.4 years

tag: Sex

value: Influenza B

tag: psofa score on day of sample 1


title: Patient number 29, Sample Collection Timepoint 2

value: 2.7 years

tag: previously healthy

tag: influenza virus result

tag: other viral co-infection

value: Other

tag: bacterial co-infection

value: ALI

tag: psofa score on day of sample 2


title: Patient number 29, Sample Collection Timepoint 1

value: blood

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

value: Influenza A - No Subtype

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: ali/ards


title: Patient number 28, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: Recovered MODS

value: 0.5 years

value: Male

value: RSV

tag: survived?


title: Patient number 28, Sample Collection Timepoint 2

value: Yes

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

tag: Sex

value: ARDS

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 27, Sample Collection Timepoint 1

value: 6.9 years

value: Female

tag: previously healthy

tag: influenza virus result

value: Influenza A H1 Pandemic

tag: other viral co-infection

tag: bacterial co-infection

tag: psofa score on day of sample 1


title: Patient number 34, Sample Collection Timepoint 2

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:Recovered (by the time second sample collected)

value: 8.1 years

value: Influenza A H3

tag: shock requiring vasopressors

value: No

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: psofa score on day of sample 2


title: Patient number 33, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: Prolonged MODS/Died

value: 13.9 years

value: Influenza B

value: MRSA or MSSA

tag: ali/ards


title: Patient number 32, Sample Collection Timepoint 1

value: blood

tag: longitudinal data

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

tag: final mods category based on manuscript definitions

value: Recovered MODS

tag: age

value: 8.2 years

tag: Sex

value: Grp A Strep or Pneumococcus

tag: survived?


title: Patient number 25, Sample Collection Timepoint 2

value: Yes

value: 12 years

value: Male

tag: previously healthy

tag: influenza virus result

tag: other viral co-infection

tag: bacterial co-infection

value: ARDS

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 23, Sample Collection Timepoint 1

value: 15 years

value: Influenza A H1 Pandemic

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: psofa score on day of sample 1


title: Patient number 26, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: Other

tag: psofa score on day of sample 2


title: Patient number 24, Sample Collection Timepoint 1

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

value: 9.7 years

tag: Sex

value: No

value: Influenza A H3

tag: ali/ards


title: Patient number 26, Sample Collection Timepoint 2

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:Recovered (by the time second sample collected)

tag: previously healthy

tag: influenza virus result

tag: other viral co-infection

tag: bacterial co-infection

tag: survived?


title: Patient number 22, Sample Collection Timepoint 2

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:Prolonged MODS/Died

value: 14.1 years

value: Influenza B

value: MRSA or MSSA

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 24, Sample Collection Timepoint 2

tag: tissue

value: blood

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

tag: psofa score on day of sample 1


title: Patient number 21, Sample Collection Timepoint 2

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

value: 15.5 years

tag: Sex

value: Female

tag: psofa score on day of sample 2


title: Patient number 22, Sample Collection Timepoint 1

value: Yes

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

value: Prolonged MODS/Died

tag: previously healthy

tag: influenza virus result

tag: other viral co-infection

tag: bacterial co-infection

tag: ali/ards


title: Patient number 27, Sample Collection Timepoint 2

value: 6.9 years

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

value: ARDS

tag: survived?


title: Patient number 19, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: 8.3 years

value: Male

value: Other

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 16, Sample Collection Timepoint 2

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

value: 14.3 years

tag: Sex

value: No

tag: psofa score on day of sample 1


title: Patient number 17, Sample Collection Timepoint 2

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:Prolonged MODS/Died

value: 16.9 years

tag: previously healthy

tag: influenza virus result

value: Influenza A H1 Pandemic

tag: other viral co-infection

tag: bacterial co-infection

tag: psofa score on day of sample 2


title: Patient number 21, Sample Collection Timepoint 1

value: Influenza B

value: MRSA or MSSA

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: ali/ards


title: Patient number 18, Sample Collection Timepoint 2

tag: tissue

value: blood

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: 12 years

value: Influenza A H3

tag: survived?


title: Patient number 20, Sample Collection Timepoint 1

value: Yes

tag: longitudinal data

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

tag: final mods category based on manuscript definitions

tag: age

value: 1.2 years

tag: Sex

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 20, Sample Collection Timepoint 2

tag: previously healthy

tag: influenza virus result

tag: other viral co-infection

tag: bacterial co-infection

value: ARDS

tag: psofa score on day of sample 1


title: Patient number 25, Sample Collection Timepoint 1

value: Prolonged MODS/Died

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: psofa score on day of sample 2


title: Patient number 18, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: Male

tag: ali/ards


title: Patient number 14, Sample Collection Timepoint 1

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

value: 16.4 years

tag: Sex

value: Female

tag: survived?


title: Patient number 15, Sample Collection Timepoint 2

value: 5.4 years

tag: previously healthy

value: No

tag: influenza virus result

tag: other viral co-infection

tag: bacterial co-infection

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 17, Sample Collection Timepoint 1

value: 16.9 years

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: psofa score on day of sample 1


title: Patient number 15, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

tag: psofa score on day of sample 2


title: Patient number 19, Sample Collection Timepoint 2

tag: longitudinal data

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:Prolonged MODS/Died

tag: final mods category based on manuscript definitions

tag: age

value: 8.3 years

tag: Sex

value: Other

tag: ali/ards


title: Patient number 13, Sample Collection Timepoint 2

value: blood

value: 9.7 years

tag: previously healthy

tag: influenza virus result

value: Influenza A - No Subtype

tag: other viral co-infection

tag: bacterial co-infection

tag: survived?


title: Patient number 23, Sample Collection Timepoint 2

value: Yes

value: 15 years

value: Influenza A H1 Pandemic

value: MRSA or MSSA

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 14, Sample Collection Timepoint 2

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: Influenza B

value: ARDS

tag: psofa score on day of sample 1


title: Patient number 13, Sample Collection Timepoint 1

tag: longitudinal data

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

tag: final mods category based on manuscript definitions

value: Prolonged MODS/Died

tag: age

tag: Sex

value: Male

tag: psofa score on day of sample 2


title: Patient number 12, Sample Collection Timepoint 2

value: 6.4 years

tag: previously healthy

value: No

tag: influenza virus result

value: Influenza A H3

tag: other viral co-infection

value: No testing

tag: bacterial co-infection

tag: ali/ards


title: Patient number 16, Sample Collection Timepoint 1

value: 14.3 years

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: survived?


title: Patient number 10, Sample Collection Timepoint 2

tag: tissue

value: blood

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:Prolonged MODS/Died

value: 11.7 years

value: Female

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 11, Sample Collection Timepoint 2

value: Yes

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

value: 12.6 years

tag: Sex

value: Grp A Strep or Pneumococcus

tag: psofa score on day of sample 1


title: Patient number 11, Sample Collection Timepoint 1

tag: previously healthy

tag: influenza virus result

value: Influenza A H1 Pandemic

tag: other viral co-infection

tag: bacterial co-infection

tag: psofa score on day of sample 2


title: Patient number 9, Sample Collection Timepoint 2

value: 5.6 years

value: Other

value: MRSA or MSSA

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: ali/ards

value: ALI


title: Patient number 10, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

value: Prolonged MODS/Died

value: ARDS

tag: survived?


title: Patient number 12, Sample Collection Timepoint 1

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

value: 6.4 years

tag: Sex

value: Male

value: No

value: Influenza A H3

value: No testing

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 9, Sample Collection Timepoint 1

value: blood

tag: previously healthy

tag: influenza virus result

tag: other viral co-infection

tag: bacterial co-infection

tag: psofa score on day of sample 1


title: Patient number 8, Sample Collection Timepoint 2

value: Yes

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:Prolonged MODS/Died

value: 10.9 years

value: Female

value: Influenza B

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: psofa score on day of sample 2


title: Patient number 8, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: Other

tag: ali/ards


title: Patient number 6, Sample Collection Timepoint 2

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: age

value: 8.2 years

tag: Sex

tag: survived?


title: Patient number 7, Sample Collection Timepoint 2

value: 12.4 years

tag: previously healthy

tag: influenza virus result

tag: other viral co-infection

tag: bacterial co-infection

value: MRSA or MSSA

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 5, Sample Collection Timepoint 2

value: 13.3 years

value: Influenza A H1 Pandemic

tag: shock requiring vasopressors

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: psofa score on day of sample 1


title: Patient number 6, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

value: Prolonged MODS/Died

tag: psofa score on day of sample 2


title: Type 2 Diabetic Nephropathy above 15 years - Clustering group C - #2

city: Taichung

first: Fuu-Jen

last: Tsai

address: No.2, Yude Rd., North Dist.

department: Department of Medical Research

country: Taiwan

institute: China Medical University Hospital

hyb_protocol: The hydridization was performed against the Clariom™ D Assay, human (Catalog Number: 902923) by following the Affymetrix protocol for 17 hours at 45°C and 60 rpm.

scan_protocol: The arrays were subsequently washed (Affymetrix Fluidics Station 450) and stained with streptavidin-phycoerythrin(GeneChip® Hybridization, Wash, and Stain Kit, 900720), and were scanned on an Affymetrix GeneChip® Scanner 3000 7G.

data_processing:
The scanning-output data, which was stored in CEL files, was analyzed by using Transcriptome Analysis Console (TAC) software of the Affymetrix company with default parameters. Moreover, uploaded CHP files were generated from the CEL files by the software.
The levels of RNA expression were determined with the Tukey biweight average scores, which was compared with eBayes, which is one of ANOVA methods, which was performanced by the TAC suite (version 4.0.2.15).

label: Biotin

source_name: Type-2 diabetic patient, with DN complication within 15 years, was assigned to group C by hierarchical clustering on principal components.

label_protocol: 300 ng of each sample was amplified and labeled using the GeneChip™ WT Sense Target Labeling and Control Reagents (Catalog number: 900652).

extract_protocol:
According to medical records of China Medical University Hospital and Changhua Christian Hospital, the selected candidates were diagnosed and classified into detailed groups non-diabetes as control, type-2 diabetes without DN/DR complications (T2D), type-2 diabetes with nephropathy (T2DN), and type-2 diabetes with retinopathy (T2DR). Based on the classifications, the blood samples of them were collected individually. Next, whole cells in the samples was accumulated by centrifugation and homogenized to prepare mixtures for RNA extraction.
Total RNA was subsequently isolated by RNeasy Mini Kit (P/N: 74104, QIAGEN) according to the manufacturer's instructions. Then, RNA quantity and purity were assessed at 260 nm and 280nm using a Nanodrop (ND-1000; Labtech. International).

value: Blood

tag: gender

tag: age

value: 62 years old

tag: t2d duration

value: 24 years

tag: hba1c (%) of the sample

tag: bmi

tag: sampling t2d stage

value: T2DN stage II (30 ≤ ACR < 300 mg/g)

tag: reference

value: Changhua Christian Hospital


title: Type 2 Diabetic Retinopathy above 15 years - Clustering group C - #3

source_name: Type-2 diabetic patient, with DR complication within 15 years, was assigned to group C by hierarchical clustering on principal components.

value: Male

value: 54 years old

value: 15 years

value: T2D with retinopathy


title: Patient number 5, Sample Collection Timepoint 1

value: blood

value: Yes

tag: longitudinal data

tag: final mods category based on manuscript definitions

tag: Sex

value: Female

tag: previously healthy

value: No

tag: influenza virus result

tag: other viral co-infection

tag: bacterial co-infection

tag: shock requiring vasopressors

tag: ali/ards

value: ARDS

tag: survived?

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 4, Sample Collection Timepoint 1

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

tag: age

value: 14.6 years

value: Influenza B

value: MRSA or MSSA

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: psofa score on day of sample 1

tag: psofa score on day of sample 2


title: Patient number 4, Sample Collection Timepoint 2

tag: longitudinal data

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:Prolonged MODS/Died

tag: final mods category based on manuscript definitions

tag: Sex

tag: previously healthy

tag: influenza virus result


title: Patient number 3, Sample Collection Timepoint 1

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

value: Prolonged MODS/Died

value: 6.2 years

value: Influenza A H1 Pandemic

tag: other viral co-infection

tag: bacterial co-infection

tag: shock requiring vasopressors

tag: ali/ards

tag: survived?


title: Patient number 3, Sample Collection Timepoint 2

tag: tissue

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: Male

tag: abs_neutrophil_count (10^3/ul)


title: Type 2 Diabetic Nephropathy above 15 years - Clustering group C - #1

value: Blood

tag: gender

tag: age

value: 81 years old

tag: t2d duration

value: 21 years

tag: hba1c (%) of the sample

tag: bmi

tag: sampling t2d stage

value: T2DN stage II (30 ≤ ACR < 300 mg/g)

tag: reference

value: Changhua Christian Hospital


title: Patient number 7, Sample Collection Timepoint 1

value: blood

value: Yes

tag: longitudinal data

tag: final mods category based on manuscript definitions

value: 12.4 years

tag: Sex

value: Female

tag: previously healthy

tag: influenza virus result

tag: other viral co-infection

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: psofa score on day of sample 1

tag: psofa score on day of sample 2


title: Type 2 Diabetic Retinopathy above 15 years - Clustering group C - #2

tag: tissue

tag: gender

tag: age

value: 69 years old

value: 17 years

value: T2D with retinopathy


title: Type 2 Diabetic Retinopathy above 15 years - Clustering group C - #1

value: 76 years old

tag: t2d duration

value: 22 years

tag: hba1c (%) of the sample

tag: bmi


title: Patient number 2, Sample Collection Timepoint 1

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

tag: longitudinal data

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

value: Prolonged MODS/Died

value: 8.4 years

value: No

value: Influenza A H3

tag: bacterial co-infection

tag: shock requiring vasopressors

tag: ali/ards

value: ARDS

tag: survived?

tag: abs_neutrophil_count (10^3/ul)


title: Patient number 2, Sample Collection Timepoint 2

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:Prolonged MODS/Died

tag: final mods category based on manuscript definitions

tag: Sex

value: Male

tag: previously healthy

tag: influenza virus result

tag: other viral co-infection

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

tag: psofa score on day of sample 1


title: Type 2 Diabetic Retinopathy above 15 years - Clustering group B - #2

source_name: Type-2 diabetic patient, with DR complication within 15 years, was assigned to group B by hierarchical clustering on principal components.

tag: tissue

value: Blood

tag: gender

tag: age

value: 66 years old

tag: t2d duration

value: 26 years

tag: hba1c (%) of the sample

tag: bmi

tag: sampling t2d stage

value: T2DN stage II (30 ≤ ACR < 300 mg/g)

tag: reference

value: Changhua Christian Hospital


title: Type 2 Diabetic Retinopathy above 15 years - Clustering group B - #1

value: 63 years old

value: 21 years


title: Patient number 1, Sample Collection Timepoint 2

value: blood

tag: patient number

tag: patient had a second sample?

value: Yes

tag: sample collection time point

tag: longitudinal data

tag: final mods category based on manuscript definitions

value: 15 years

value: Other

tag: bacterial co-infection

value: MRSA or MSSA

tag: shock requiring vasopressors

tag: ali/ards

tag: survived?

tag: abs_neutrophil_count (10^3/ul)

tag: psofa score on day of sample 2


title: Type 2 Diabetes without complications above 15 years - Clustering group C - #5

source_name: Type-2 diabetic patient, without DN and DR complications within 15 years, was assigned to group C by hierarchical clustering on principal components.

tag: tissue

tag: gender

tag: age

value: 67 years old

value: 11 years

value: T2D without complications (T2D duration ≥ 10 years & HbA1c ≥ 7%)


title: Type 2 Diabetic Nephropathy above 15 years - Clustering group B - #2

source_name: Type-2 diabetic patient, with DN complication within 15 years, was assigned to group B by hierarchical clustering on principal components.

value: 42 years old

tag: t2d duration

value: around 15 years

tag: hba1c (%) of the sample

tag: bmi


title: Type 2 Diabetes without complications above 15 years - Clustering group C - #3

value: Blood

value: Male

value: 60 years old

tag: sampling t2d stage

tag: reference


title: Patient number 1, Sample Collection Timepoint 1

tag: patient number

tag: patient had a second sample?

tag: sample collection time point

value: MODS STATUS AT THE TIME OF SECOND SAMPLE:n/a

value: Prolonged MODS/Died

tag: Sex

tag: previously healthy

tag: influenza virus result

value: Influenza A H3

tag: other viral co-infection

tag: invasive mechanical ventilation

tag: ecmo support

tag: total hours in icu

value: ARDS

value: No

tag: psofa score on day of sample 1


title: Type 2 Diabetes without complications above 15 years - Clustering group C - #4

tag: tissue

tag: gender

tag: age

value: 61 years old

value: 12 years

value: T2D without complications (T2D duration ≥ 10 years & HbA1c < 7%)

value: Changhua Christian Hospital


title: Type 2 Diabetes without complications above 15 years - Clustering group C - #2

value: 51 years old

tag: t2d duration

tag: hba1c (%) of the sample

tag: bmi


title: Type 2 Diabetic Nephropathy above 15 years - Clustering group B - #1

value: Female

value: 74 years old

tag: sampling t2d stage

value: T2DN stage II (30 ≤ ACR < 300 mg/g)

tag: reference


title: Type 2 Diabetes without complications above 15 years - Clustering group B - #4

source_name: Type-2 diabetic patient, without DN and DR complications within 15 years, was assigned to group B by hierarchical clustering on principal components.

value: 73 years old

value: 19 years

value: T2D without complications (T2D duration ≥ 10 years & HbA1c ≥ 7%)


title: Type 2 Diabetes without complications above 15 years - Clustering group B - #3

value: Blood

value: Male

value: 63 years old

value: around 15 years


title: Type 2 Diabetes without complications above 15 years - Clustering group B - #2

value: 66 years old

value: 17 years


title: Type 2 Diabetes without complications above 15 years - Clustering group B - #1

value: 56 years old

value: 20 years


title: Type 2 Diabetic Retinopathy within 5 years - Clustering group C - #3

source_name: Type-2 diabetic patient, with DR complication within 5 years, was assigned to group C by hierarchical clustering on principal components.

value: 43 years old

value: 1 year

value: T2D with retinopathy


title: Type 2 Diabetic Nephropathy within 5 years - Clustering group C - #2

source_name: Type-2 diabetic patient, with DN complication within 5 years, was assigned to group C by hierarchical clustering on principal components.

value: 62 years old

value: 5 years

value: Changhua Christian Hospital


title: Type 2 Diabetic Retinopathy within 5 years - Clustering group C - #1

value: 59 years old


title: Type 2 Diabetic Retinopathy within 5 years - Clustering group B - #2

source_name: Type-2 diabetic patient, with DR complication within 5 years, was assigned to group B by hierarchical clustering on principal components.

value: Female

value: 77 years old

value: 2 years


title: Type 2 Diabetic Nephropathy within 5 years - Clustering group C - #1

value: Blood

value: Male

value: 57 years old

value: 4 years

value: T2DN stage III (300 ≤ ACR < 2000 mg/g)


title: Type 2 Diabetic Nephropathy within 5 years - Clustering group B - #2

source_name: Type-2 diabetic patient, with DN complication within 5 years, was assigned to group B by hierarchical clustering on principal components.

value: 49 years old

value: T2DN stage II (30 ≤ ACR < 300 mg/g)


title: Type 2 Diabetic Retinopathy within 5 years - Clustering group B - #1

value: less than 1 year


title: Type 2 Diabetes without complications within 5 years - Clustering group C - #2

source_name: Type-2 diabetic patient, without DN and DR complications within 5 years, was assigned to group C by hierarchical clustering on principal components.

value: 40 years old

value: T2D without complications (T2D duration < 5 years)


title: Type 2 Diabetic Retinopathy within 5 years - Clustering group C - #2

value: 63 years old

value: T2D with retinopathy


title: Type 2 Diabetes without complications within 5 years - Clustering group C - #5

value: 62 years old

value: 6 years

value: T2D without complications (5years ≤ T2D duration < 10 years)

value: Changhua Christian Hospital


title: Type 2 Diabetic Nephropathy within 5 years - Clustering group B - #1

value: Female

value: 72 years old

value: 5 years


title: Type 2 Diabetes without complications within 5 years - Clustering group C - #3

value: Blood

value: Male


title: Type 2 Diabetes without complications within 5 years - Clustering group C - #1

value: 55 years old

value: 3 years


title: Type 2 Diabetes without complications within 5 years - Clustering group C - #4

value: 66 years old


title: Type 2 Diabetes without complications within 5 years - Clustering group B - #4

source_name: Type-2 diabetic patient, without DN and DR complications within 5 years, was assigned to group B by hierarchical clustering on principal components.

value: 44 years old

value: 1 year


title: Type 2 Diabetes without complications within 5 years - Clustering group B - #3

value: T2D without complications (T2D duration < 5 years)


title: Type 2 Diabetes without complications within 5 years - Clustering group B - #2

value: 54 years old

value: less than 1 year


title: Control - Clustering group A - #8

source_name: Non-diabetic patient was assigned to group A or control group by hierarchical clustering on principal components.

value: 46 years old

value: Not applicable

value: Between 4.0% and 5.6%

value: China Medical University Hospital


title: Control - Clustering group A - #7

value: 41 years old


title: Control - Clustering group A - #6

value: Female

value: 43 years old


title: Control - Clustering group A - #5

value: Blood


title: Control - Clustering group A - #4

value: Male

value: 42 years old


title: Control - Clustering group A - #3


title: Type 2 Diabetes without complications above 15 years - Clustering group C - #1

value: 61 years old

value: 15 years

value: T2D without complications (T2D duration ≥ 10 years & HbA1c < 7%)

value: Changhua Christian Hospital


title: Control - Clustering group A - #1

value: 47 years old


title: Control - Clustering group A - #2

value: 54 years old


title: A375Vem rep3

city: Dongguan

first: XUERONG

last: SUN

state: Guangdong Province

address: No. 1 Xincheng Avenue, Songshan Lake District

institute: Guangdong Medical University

description: fpkm_A375Vem3, tpm_A375Vem3, read_count_A375Vem3

data_processing:
HISAT2 (v2.0.4) was used for reference genome alignment
Sequence reads were trimmed for adaptor sequence/low-quality sequence using SOAPnuke (v1.5.2)
Assembly: hg38
Supplementary files format and content: tab-delimited text files include FPKM, TPM and read count values for all samples

source_name: skin

growth_protocol: A375 cells were cultured in in Dulbecco’s Modified Eagle Medium (DMEM) with 10% fetal bovine serum.

extract_protocol:
Total RNA was extracted using Trizol Reagent (Invitrogen, USA)
Then First-strand cDNA was generated using random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis. Afterwards, A-Tailing Mix and RNA Index Adapters were added by incubating to end repair. The cDNA fragments were obtained from previous step were amplified by PCR, and products were purified by Ampure XP Beads, then dissolved in EB solution. The product was validated on the Agilent Technologies 2100 bioanalyzer for quality control. The double stranded PCR products from previous step were heated denatured and circularized by the splint oligo sequence to get the final library. The single strand circle DNA was formatted as the final library.

treatment_protocol: Melanoma A375 cells were treated with vemurafenib for 3 days or with cisplatin for 1 day, and then returned to normal culture until RNA-seq on day 7.

value: skin

tag: cell line

tag: cell type

value: Melanoma cells

tag: genotype

tag: treatment

value: Vemurafenib


title: A375CDDP rep3

description: fpkm_A375CDDP3, tpm_A375CDDP3, read_count_A375CDDP3

value: Cisplatin


title: A375CDDP rep1

description: fpkm_A375CDDP1, tpm_A375CDDP1, read_count_A375CDDP1


title: A375CDDP rep2

description: fpkm_A375CDDP2, tpm_A375CDDP2, read_count_A375CDDP2


title: A375Vem rep2

description: fpkm_A375Vem2, tpm_A375Vem2, read_count_A375Vem2


title: A375NC rep3

description: fpkm_A375NC3, tpm_A375NC3, read_count_A375NC3

value: Normal culture


title: A375NC rep2

description: fpkm_A375NC2, tpm_A375NC2, read_count_A375NC2


title: A375NC rep1

description: fpkm_A375NC1, tpm_A375NC1, read_count_A375NC1


title: A375Vem rep1

description: fpkm_A375Vem1, tpm_A375Vem1, read_count_A375Vem1


title: Type 2 Diabetes without complications within 5 years - Clustering group B - #1

value: 63 years old

value: 1 year

value: T2D without complications (T2D duration < 5 years)


title: Control - Clustering group A - #9

value: Blood

value: Female

value: 40 years old

value: Not applicable

value: Between 4.0% and 5.6%

value: China Medical University Hospital


title: 8A_M_3051

city: La Jolla

first: Joseph

middle: R

last: Ecker

state: CA

address: 10010 North Torrey Pines Road

department: Genomic Analysis Laboratory

institute: HHMI-Salk-Institute

data_processing:
Reads trimed with Cutadapt for adapters and low quality bases (Phred score >= 20)
The resulting files are mapped to in-silico bisulfite converted mm10 reference genome using Bismark.
The raw BAM file will be filtered using samtools to filter reads by MAPQ > 10 and use Picard to remove PCR duplicates
Tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls will be generated with in-house developing package using the filtered BAM files
Assembly: mm10
Supplementary files format and content: tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls. Columns are: 1. Chromosome, 2. Position, 3. Strand, 4. Cytosin Context, 5. methylated base call, 6. total mapped base call, 7. not used, place holder column
Library strategy: snmC-seq2

source_name: C57BL/6J Male P56 Mouse Brain

growth_protocol: All mice are C57BL/6J Male, brain dissected in P56

extract_protocol:
the brain of P56 male C57BL/6J mouse will be removed and cut into 600 microns thick coronal sections using a tissue matrix. Then each region will be microdissected from the coronal tissue sections based on identified landmarks and brain atlas coordinates, as defined in Allen brain atlas CCF3 (Avaliable at: http://atlas.brain-map.org). For each dissection region, the tissue from several littermate mice will be combined together into to separate replicate groups. The number of animals in a replicate depends on the size of the tissue. Nuclei preparation will be stained with the fluorescently labeled anti-NeuN body and nucleic acid stain Hoechst 33342.
Library prepared following protocol discribed in Luo, Chongyuan, Angeline Rivkin, Jingtian Zhou, Justin P. Sandoval, Laurie Kurihara, Jacinta Lucero, Rosa Castanon, et al. 2018. “Robust Single-Cell DNA Methylome Profiling with snmC-seq2.” Nature Communications 9 (1): 3824.

tag: brain region

tag: strain


title: 8A_M_3052


title: 8A_M_3054


title: 8A_M_3053


title: 8A_M_3059


title: 8A_M_3058


title: 8A_M_3057


title: 8A_M_3047


title: 8A_M_3050


title: 8A_M_3046


title: 8A_M_3049


title: 8A_M_3055


title: 8A_M_3048


title: 8A_M_3043


title: 8A_M_3042


title: 8A_M_3060


title: 8A_M_3044


title: 8A_M_3039


title: 8A_M_3040


title: 8A_M_3038


title: 8A_M_3041


title: 8A_M_3056


title: 8A_M_3036


title: 8A_M_3034


title: 8A_M_3035


title: 8A_M_3031


title: 8A_M_3033


title: 8A_M_3032


title: 8A_M_3045


title: 8A_M_3026


title: 8A_M_3028


title: 8A_M_3024


title: 8A_M_3022


title: 8A_M_3030


title: 8A_M_3037


title: 8A_M_3021


title: 8A_M_3029


title: 8A_M_3017


title: 8A_M_3023


title: 8A_M_3015


title: 8A_M_3014


title: 8A_M_3020


title: 8A_M_3025


title: 8A_M_3018


title: 8A_M_3011


title: 8A_M_3019


title: 8A_M_3016


title: 8A_M_3013


title: 8A_M_3010


title: 8A_M_3005


title: 8A_M_3012


title: 8A_M_3006


title: 8A_M_3007


title: 8A_M_3002


title: 8A_M_3008


title: 8A_M_3009


title: 8A_M_3003


title: 8A_M_3004


title: 8A_M_3027


title: 8A_M_3000


title: 8A_M_2996


title: 8A_M_2994


title: 8A_M_3001


title: 8A_M_2998


title: 8A_M_2999


title: 8A_M_2987


title: 8A_M_2984


title: 8A_M_2993


title: 8A_M_2991


title: 8A_M_2989


title: 8A_M_2995


title: 8A_M_2981


title: 8A_M_2983


title: 8A_M_2997


title: 8A_M_2990


title: 8A_M_2986


title: 8A_M_2974


title: 8A_M_2980


title: 8A_M_2979


title: 8A_M_2976


title: 8A_M_2977


title: 8A_M_2985


title: 8A_M_2988


title: 8A_M_2972


title: 8A_M_2975


title: 8A_M_2973


title: 8A_M_2992


title: 8A_M_2982


title: 8A_M_2970


title: 8A_M_2968


title: 8A_M_2969


title: 8A_M_2978


title: 8A_M_2966


title: 8A_M_2962


title: 8A_M_2963


title: 8A_M_2965


title: 8A_M_2964


title: 8A_M_2957


title: 8A_M_2956


title: 8A_M_2960


title: 8A_M_2958


title: 8A_M_2959


title: 8A_M_2952


title: 8A_M_2953


title: 8A_M_2949


title: 8A_M_2950


title: 8A_M_2948


title: 8A_M_2955


title: 8A_M_2954


title: 8A_M_2951


title: 8A_M_2947


title: 8A_M_2946


title: 8A_M_2944


title: 8A_M_2971


title: 8A_M_2967


title: 8A_M_2943


title: 8A_M_2961


title: 8A_M_2942


title: 8A_M_2941


title: 8A_M_2939


title: 8A_M_2938


title: 8A_M_2940


title: 8A_M_2937


title: 8A_M_2933


title: 8A_M_2936


title: 8A_M_2935


title: 8A_M_2932


title: 8A_M_2930


title: 8A_M_2929


title: 8A_M_2927


title: 8A_M_2923


title: 8A_M_2925


title: 8A_M_2919


title: 8A_M_2921


title: 8A_M_2924


title: 8A_M_2926


title: 8A_M_2945


title: 8A_M_2920


title: 8A_M_2917


title: 8A_M_2918


title: 8A_M_2922


title: 8A_M_2915


title: 8A_M_2916


title: 8A_M_2913


title: 8A_M_2914


title: 8A_M_2934


title: 8A_M_2931


title: 8A_M_2911


title: 8A_M_2910


title: 8A_M_2928


title: 8A_M_2909


title: 8A_M_2908


title: 8A_M_2907


title: 8A_M_2906


title: 8A_M_2904


title: 8A_M_2903


title: 8A_M_2901


title: 8A_M_2902


title: 8A_M_2899


title: 8A_M_2900


title: 8A_M_2898


title: 8A_M_2897


title: 8A_M_2890


title: 8A_M_2892


title: 8A_M_2893


title: 8A_M_2896


title: 8A_M_2891


title: 8A_M_2912


title: 8A_M_2889


title: 8A_M_2885


title: 8A_M_2888


title: 8A_M_2905


title: 8A_M_2883


title: 8A_M_2884


title: 8A_M_2886


title: 8A_M_2887


title: 8A_M_2882


title: 8A_M_2879


title: 8A_M_2877


title: 8A_M_2880


title: 8A_M_2875


title: 8A_M_2881


title: 8A_M_2894


title: 8A_M_2878


title: 8A_M_2872


title: 8A_M_2871


title: 8A_M_2870


title: 8A_M_2869


title: 8A_M_2867


title: 8A_M_2864


title: 8A_M_2865


title: 8A_M_2866


title: 8A_M_2863


title: 8A_M_2861


title: 8A_M_2862


title: 8A_M_2859


title: 8A_M_2860


title: 8A_M_2856


title: 8A_M_2857


title: 8A_M_2853


title: 8A_M_2873


title: 8A_M_2851


title: 8A_M_2858


title: 8A_M_2855


title: 8A_M_2847


title: 8A_M_2848


title: 8A_M_2850


title: 8A_M_2849


title: 8A_M_2868


title: 8A_M_2854


title: 8A_M_2844


title: 8A_M_2845


title: 8A_M_2842


title: 8A_M_2876


title: 8A_M_2841


title: 8A_M_2839


title: 8A_M_2838


title: 8A_M_2837


title: 8A_M_2836


title: 8A_M_2835


title: 8A_M_2840


title: 8A_M_2852


title: 8A_M_2846


title: 8A_M_2832


title: 8A_M_2831


title: 8A_M_2830


title: 8A_M_2829


title: 8A_M_2874


title: 8A_M_2833


title: 8A_M_2825


title: 8A_M_2823


title: 8A_M_2822


title: 8A_M_2834


title: 8A_M_2843


title: 8A_M_2820


title: 8A_M_2821


title: 8A_M_2819


title: 8A_M_2817


title: 8A_M_2818


title: 8A_M_2816


title: 8A_M_2815


title: 8A_M_2814


title: 8A_M_2813


title: 8A_M_2811


title: 8A_M_2810


title: 8A_M_2812


title: 8A_M_2808


title: 8A_M_2828


title: 8A_M_2806


title: 8A_M_2807


title: 8A_M_2824


title: 8A_M_2803


title: 8A_M_2805


title: 8A_M_2800


title: 8A_M_2804


title: 8A_M_2799


title: 8A_M_2801


title: 8A_M_2796


title: 8A_M_2795


title: 8A_M_2797


title: 8A_M_2793


title: 8A_M_2798


title: 8A_M_2794


title: 8A_M_2791


title: 8A_M_2789


title: 8A_M_2790


title: 8A_M_2792


title: 8A_M_2788


title: 8A_M_2809


title: 8A_M_2787


title: 8A_M_2786


title: 8A_M_2785


title: 8A_M_2781


title: 8A_M_2782


title: 8A_M_2784


title: 8A_M_2783


title: 8A_M_2779


title: 8A_M_2777


title: 8A_M_2778


title: 8A_M_2780


title: 8A_M_2775


title: 8A_M_2774


title: 8A_M_2776


title: 8A_M_2771


title: 8A_M_2772


title: 8A_M_2768


title: 8A_M_2766


title: 8A_M_2770


title: 8A_M_2773


title: 8A_M_2767


title: 8A_M_2765


title: 8A_M_2764


title: 8A_M_2762


title: 8A_M_2761


title: 8A_M_2758


title: 8A_M_2759


title: 8A_M_2760


title: 8A_M_2756


title: 8A_M_2755


title: 8A_M_2754


title: 8A_M_2753


title: 8A_M_2750


title: 8A_M_2751


title: 8A_M_2769


title: 8A_M_2746


title: 8A_M_2749


title: 8A_M_2752


title: 8A_M_2747


title: 8A_M_2763


title: 8A_M_2748


title: 8A_M_2745


title: 8A_M_2742


title: 8A_M_2744


title: 8A_M_2743


title: 8A_M_2737


title: 8A_M_2740


title: 8A_M_2739


title: 8A_M_2741


title: 8A_M_2735


title: 8A_M_2757


title: 8A_M_2729


title: 8A_M_2733


title: 8A_M_2734


title: 8A_M_2732


title: 8A_M_2728


title: 8A_M_2731


title: 8A_M_2727


title: 8A_M_2721


title: 8A_M_2726


title: 8A_M_2720


title: 8A_M_2719


title: 8A_M_2724


title: 8A_M_2718


title: 8A_M_2736


title: 8A_M_2722


title: 8A_M_2723


title: 8A_M_2717


title: 8A_M_2714


title: 8A_M_2713


title: 8A_M_2827


title: 8A_M_2738


title: 8A_M_2716


title: 8A_M_2715


title: 8A_M_2710


title: 8A_M_2712


title: 8A_M_2709


title: 8A_M_2711


title: 8A_M_2708


title: 8A_M_2705


title: 8A_M_2697


title: 8A_M_2698


title: 8A_M_2704


title: 8A_M_2700


title: 8A_M_2707


title: 8A_M_2690


title: 8A_M_2695


title: 8A_M_2689


title: 8A_M_2694


title: 8A_M_2688


title: 8A_M_2692


title: 8A_M_2696


title: 8A_M_2693


title: 8A_M_2691


title: 8A_M_2687


title: 8A_M_2686


title: 8A_M_2683


title: 8A_M_2684


title: 8A_M_2682


title: 8A_M_2685


title: 8A_M_2681


title: 8A_M_2702


title: 8A_M_2678


title: 8A_M_2680


title: 8A_M_2679


title: 8A_M_2677


title: 8A_M_2675


title: 8A_M_2699


title: 8A_M_2672


title: 8A_M_2673


title: 8A_M_2674


title: 8A_M_2671


title: 8A_M_2670


title: 8A_M_2826


title: 8A_M_2666


title: 8A_M_2664


title: 8A_M_2669


title: 8A_M_2662


title: 8A_M_2665


title: 8A_M_2663


title: 8A_M_2659


title: 8A_M_2657


title: 8A_M_2676


title: 8A_M_2668


title: 8A_M_2654


title: 8A_M_2656


title: 8A_M_2660


title: 8A_M_2658


title: 8A_M_2653


title: 8A_M_2652


title: 8A_M_2647


title: 8A_M_2651


title: 8A_M_2650


title: 8A_M_2648


title: 8A_M_2646


title: 8A_M_2644


title: 8A_M_2649


title: 8A_M_2641


title: 8A_M_2640


title: 8A_M_2645


title: 8A_M_2642


title: 8A_M_2661


title: 8A_M_2655


title: 8A_M_2638


title: 8A_M_2636


title: 8A_M_2634


title: 8A_M_2637


title: 8A_M_2628


title: 8A_M_2639


title: 8A_M_2631


title: 8A_M_2632


title: 8A_M_2630


title: 8A_M_2629


title: 8A_M_2626


title: 8A_M_2635


title: 8A_M_2627


title: 8A_M_2620


title: 8A_M_2619


title: 8A_M_2618


title: 8A_M_2623


title: 8A_M_2643


title: 8A_M_2622


title: 8A_M_2624


title: 8A_M_2621


title: 8A_M_2617


title: 8A_M_2615


title: 8A_M_2633


title: 8A_M_2613


title: 8A_M_2616


title: 8A_M_2612


title: 8A_M_2625


title: 8A_M_2607


title: 8A_M_2609


title: 8A_M_2611


title: 8A_M_2601


title: 8A_M_2602


title: 8A_M_2605


title: 8A_M_2598


title: 8A_M_2597


title: 8A_M_2599


title: 8A_M_2600


title: 8A_M_2606


title: 8A_M_2593


title: 8A_M_2591


title: 8A_M_2592


title: 8A_M_2595


title: 8A_M_2590


title: 8A_M_2596


title: 8A_M_2589


title: 8A_M_2594


title: 8A_M_2587


title: 8A_M_2614


title: 8A_M_2583


title: 8A_M_2585


title: 8A_M_2584


title: 8A_M_2586


title: 8A_M_2582


title: 8A_M_2608


title: 8A_M_2604


title: 8A_M_2581


title: 8A_M_2580


title: 8A_M_2579


title: 8A_M_2578


title: 8A_M_2577


title: 8A_M_2576


title: 8A_M_2588


title: 8A_M_2574


title: 8A_M_2575


title: 8A_M_2561


title: 8A_M_2573


title: 8A_M_2563


title: 8A_M_2560


title: 8A_M_2564


title: 8A_M_2559


title: 8A_M_2572


title: 8A_M_2557


title: 8A_M_2570


title: 8A_M_2554


title: 8A_M_2571


title: 8A_M_2569


title: 8A_M_2568


title: 8A_M_2567


title: 8A_M_2552


title: 8A_M_2565


title: 8A_M_2550


title: 8A_M_2558


title: 8A_M_2555


title: 8A_M_2549


title: 8A_M_2553


title: 8A_M_2547


title: 8A_M_2548


title: 8A_M_2546


title: 8A_M_2566


title: 8A_M_2562


title: 8A_M_2556


title: 8A_M_2541


title: 8A_M_2545


title: 8A_M_2540


title: 8A_M_2544


title: 8A_M_2539


title: 8A_M_2542


title: 8A_M_2538


title: 8A_M_2534


title: 8A_M_2551


title: 8A_M_2535


title: 8A_M_2536


title: 8A_M_2531


title: 8A_M_2529


title: 8A_M_2525


title: 8A_M_2526


title: 8A_M_2527


title: 8A_M_2524


title: 8A_M_2522


title: 8A_M_2521


title: 8A_M_2533


title: 8A_M_2532


title: 8A_M_2528


title: 8A_M_2523


title: 8A_M_2543


title: 8A_M_2520


title: 8A_M_2518


title: 8A_M_2519


title: 8A_M_2517


title: 8A_M_2537


title: 8A_M_2516


title: 8A_M_2510


title: 8A_M_2514


title: 8A_M_2513


title: 8A_M_2512


title: 8A_M_2530


title: 8A_M_2508