title: Mouse gut feces, CON diet (zr4773_14V3V4)

city: Chicago

first: fan

last: hao

address: 5801 S Ellis Ave

institute: University of Chicago

data_processing:
The final library was sequenced on Illumina® MiSeq™ with a v3 reagent kit (600 cycles). The sequencing was performed with 10% PhiX spike-in.
Unique amplicon sequences variants were inferred from raw reads using the DADA2 pipeline
Taxonomy assignment was performed using Uclust from Qiime v.1.9.1 with the Zymo Research Database, a 16S database that is internally designed and curated
Composition visualization, alpha-diversity, and beta-diversity analyses were performed with Qiime v.1.9.1
Assembly: Zymo Research Database, a 16S database that is internally designed and curated
Supplementary files format and content: tab-delimited text files include taxonomy abundance for each Sample

organism: feces metagenome

source_name: feces

extract_protocol:
ZymoBIOMICS®-96 MagBead DNA Kit (Zymo Research, Irvine, CA) was used to extract DNA using an automated platform.
Bacterial 16S ribosomal RNA gene targeted sequencing was performed using the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA). In most cases, the bacterial 16S primers amplified the V3-V4 region of the 16S rRNA gene.
The sequencing library was prepared using an innovative library preparation process in which PCR reactions were performed in real-time PCR machines to control cycles and therefore limit PCR chimera formation. The final PCR products were quantified with qPCR fluorescence readings and pooled together based on equal molarity. The final pooled library was cleaned with the Select-a-Size DNA Clean & Concentrator™ (Zymo Research, Irvine, CA), then quantified with TapeStation®(Agilent Technologies, Santa Clara, CA) and Qubit® (Thermo Fisher Scientific, Waltham, WA).

value: Mouse

tag: rna population

value: 16S rRNA

value: CON diet

tag: tissue

value: feces


title: Mouse gut feces, CON diet (zr4773_13V3V4)

status: Public on Sep 21 2023

type: SRA

state: IL

country: USA

library_source: genomic

molecule: genomic DNA

tag: source

tag: treatment


title: Mouse gut feces, CON diet (zr4773_12V3V4)


title: Mouse gut feces, CON diet (zr4773_11V3V4)


title: Mouse gut feces, TVA diet (zr4773_7V3V4)

value: TVA diet


title: Mouse gut feces, CON diet (zr4773_8V3V4)


title: Mouse gut feces, TVA diet (zr4773_6V3V4)


title: Mouse gut feces, CON diet (zr4773_9V3V4)


title: Mouse gut feces, CON diet (zr4773_10V3V4)


title: obob_alpha, biological replicate 3 (RNA-seq)

city: Davis

first: Mark

middle: O.

last: Huising

state: CA

address: One Shields Avenue

department: Neurobiology, Physiology and Behavior

institute: University of California, Davis

description: bulk RNA expression

library_source: transcriptomic

data_processing:
FastQC
Trimmomatic
STAR Aligner
edgeR
Assembly: mm10
Supplementary files format and content: Raw Expression Counts

molecule: total RNA

organism: Mus musculus

source_name: FACS-sorted alpha cells

growth_protocol: Pancreatic islets were isolated by injection of collagenase up the common bile duct after clamping of the Ampulla of Vater. Isolates were purified by density gradient centrifugation and dissociated by gentle trituration in 0.25% Trypsin -EDTA. mIns1-H2b-mCherry positive beta cells were collected and processed immediately for ATAC-Seq library preparation.

extract_protocol:
Cells were collected in Trizol post FACS purification and isolated by a chloroform extraction, assisted by phase lock tubes. RNA was precipitated by isopropanol and cleaned up over an RNEasy microcolumn per the Qiagen's taking great care to avoid carry over of ethanol following the column washes. Elution volume was 30 microliter.
Indexed sequencing libraries were constructed using the TruSeq RNA sample prep Kit v2 per Illumina instructions.

tag: cell type

value: alpha

tag: Sex

value: male

tag: status

value: obob


title: lean_alpha, biological replicate 7 (RNA-seq)

description: bulk RNA expression

value: lean


title: Mouse gut feces, TVA diet (zr4773_4V3V4)


title: Mouse gut feces, TVA diet (zr4773_2V3V4)


title: Mouse gut feces, TVA diet (zr4773_5V3V4)


title: Mouse gut feces, TVA diet (zr4773_1V3V4)


title: lean_beta, biological replicate 2 (RNA-seq)

description: bulk RNA expression

source_name: FACS-sorted beta cells

value: beta

value: female


title: lean_alpha, biological replicate 3 (RNA-seq)

description: bulk RNA expression


title: Mouse gut feces, TVA diet (zr4773_3V3V4)


title: ID 230111-4_mock_d36_Bulk

type: RNA

city: Hannover

first: Philipp

middle: Walter

last: John-Neek

state: Lower Saxony

department: Institute of Experimental Hematology

country: Germany

institute: Medizinische Hochschule Hannover (MHH)

hyb_protocol: After labeling, cRNA fragmentation, hybridization, and washing steps were carried out as recommended in ‘One-Color Microarray-Based Gene Expression Analysis Protocol V5.7’, except that 500 ng of each fluorescently labeled cRNA population were used for hybridization.

scan_protocol: The hybridized arrays were washed, fixed and the slides were scanned using the Agilent Micro Array Scanner G2565CA (pixel resolution 3 μm, bit depth 20). Data extraction was performed with the ‘FeatureExtraction Software V10.7.3.1’ using the extraction protocol file ‘GE1_107_Sep09.xml’.

data_processing: The extracted features were analyzed following the protocol described on the SAGA homepage (https://talbotsr.com/saga_package/index.html), using R studio. Briefly, the raw data were log2-transformed, quantile-normalized, and within-array the probe replicates were averaged using the R package limma. The ComBat algorithm of the R package “sva” was used for batch correction between different experimental batches. Results of further gene expression analysis were visualized as a 2D principal component analysis (PCA) plot. All calculations were performed in R 4.0.5 on a server running in Ubuntu 20.04.3

label: Alexa Fluor 555

label_protocol: Microarrays using the isolated RNA were performed by the Research Core Unit Genomics team of Hannover Medical School and as previously described in detail in Schwarzer et al. 2021. Briefly, when possible 100 ng (or less if not available) of total RNA were used to prepare Aminoallyl-UTP-modified (aaUTP) cRNA (Amino Allyl MessageAmp™ II Kit; Thermo Fisher Scientific), applying one round of amplification as directed by the company, except for a two-fold downscaling of all reaction volumes. Before the reverse transcription reaction, 1 μL of 1:5000 dilution of Agilent’s One-Color spike-in Kit stock solution (Agilent Technologies) was added to the total RNA used for each sample. The labeling of aaUTP-cRNA was performed with Alexa Fluor 555 Reactive Dye (Thermo Fisher Scientific) following the manufacturer’s instructions with the Amino Allyl MessageAmp™ II Kit (two-fold downscaled reaction volumes).

growth_protocol: Lineage negative cells were isolated from 8-12-week old female C57BL/6J mice by magnetic separation using the lineage cell depletion kit (Miltenyi). The cells were thawed and cultured under lymphoid cytokine conditions (alpha-MEM basal medium supplemented with 100 U/mL penicillin, 100 µg/mL streptomycin, 20% fetal bovine serum, and the citokines IL-7, mSCF, and Flt-3L) using the OP9-DL1 co-culturing system. Every 3-4 days, the lineage negative cells were replated to a new layer of concluent OP9-DL1 cells.

extract_protocol: Cells were harvested, centrifuged down at 300 xg, 4°C, for 5 min, resuspended thoroughly in RNAzol B (WAK-Chemie Medical GmbH), and stored at -80°C for later use. RNA was isolated using the Direct-zol RNA mini- or microkit (Zymo Research), depending on the number of input cells (microkit: < 1.00x10^5 cells), following the manufacturer´s instructions. RNA was eluted in 30 µL (mini) or 15 µL (micro) of DNase/RNase-free water and quantified by Nanodrop before storing at -80°C. Prior to processing for microarray, the RNA quality and concentration was assessed using the Agilent 2100 Bioanalyzer.

treatment_protocol: For each experiment, the lineage negative cells were thawed and pre-stimulated for 24 h before transduction in StemSpan SFEM (STEMCELL Technologies) supplemented with 5 ng/mL human/murine IL-7, 5 ng/mL hFLT-3L, 50 ng/mL mSCF, 20 ng/mL mTPO, 20ng/mL mIGF2, and 10 ng/mL hFGF1. Transduction of 1.00x10^5 cells with the retroviral vectors were performed on 10 µg/cm2 RetroNectin-coated 24-well plates using the same medium as the pre-stimulation step, with additional 4 µg/mL of protamine sulfate. One day after transduction, the cells were transferred to a layer of confluent OP9-DL1 mitomycin C (MMC)-treated cells, seeded the day before, and the medium was changed to alpha-MEM with 100 U/mL penicillin, 100 µg/mL streptomycin, 20% fetal bovine serum, 1 ng/mL IL-7, 5 ng/mL mSCF and 5 ng/mL hFLT-3L. The lin- cells were transferred to new layers of OP9-DL1 MMC-treated cells every 3-4 days. On day 12, the IL-7 concentration was reduced to 0.5 ng/mL and on day 15, the mSCF was reduced to 5 ng/mL. Cell material was harvested when possible for later DNA and RNA isolation and downstream analysis.

tag: design

tag: class

value: untransforming

tag: strain

tag: batch


title: ID 230111-17_RAG1_d36_Bulk


title: ID 230111-3_mock_d36_Bulk


title: ID 230111-10_EFS_d36_Bulk


title: ID 230111-8_EFS_d36_Bulk


title: ID 230111-12_SIN-LV.SF_d36_Bulk

value: transforming


title: ID 230111-7_RSF91_d36_Bulk


title: ID 230111-11_SIN-LV.SF_d36_Bulk


title: ID 230111-15_RAG2_d29_Bulk


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title: ID 230111-6_RSF91_d36_Bulk


title: ID 230111-2_mock_d36_Bulk


title: ID 230111-19_RAG1_d36_Bulk


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title: ID 200131-7_RSF91_d36_Bulk


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title: ID 210527-7_RSF91_d36_Bulk


title: ID 210527-9_RSF91_d36_Bulk


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title: ID 200131-2_mock_d40_Bulk


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title: ID 200131-4_EFS_d40_Bulk


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title: ID 190614-5_RSF91_d29_Bulk


title: ID 210527-15_RAG1_d26_DN2

treatment_protocol: For each experiment, the lineage negative cells were thawed and pre-stimulated for 24 h before transduction in StemSpan SFEM (STEMCELL Technologies) supplemented with 5 ng/mL human/murine IL-7, 5 ng/mL hFLT-3L, 50 ng/mL mSCF, 20 ng/mL mTPO, 20ng/mL mIGF2, and 10 ng/mL hFGF1. Transduction of 1.00x10^5 cells with the retroviral vectors were performed on 10 µg/cm2 RetroNectin-coated 24-well plates using the same medium as the pre-stimulation step, with additional 4 µg/mL of protamine sulfate. One day after transduction, the cells were transferred to a layer of confluent OP9-DL1 mitomycin C (MMC)-treated cells, seeded the day before, and the medium was changed to alpha-MEM with 100 U/mL penicillin, 100 µg/mL streptomycin, 20% fetal bovine serum, 1 ng/mL IL-7, 5 ng/mL mSCF and 5 ng/mL hFLT-3L. The lin- cells were transferred to new layers of OP9-DL1 MMC-treated cells every 3-4 days. On day 12, the IL-7 concentration was reduced to 0.5 ng/mL and on day 15, the mSCF was reduced to 5 ng/mL. Cell material was harvested when possible for later DNA and RNA isolation and downstream analysis. On days 25-26 the cells were sorted based on their CD44/CD25/CD4/CD8/Gr-1/CD11b marker expression. The DN2 cells (CD44+/CD25+/CD4-/CD8-/CD11b-/Gr-1-) were centrifuged, resuspended in 300 µL of RNAzol and stored at -80°C for further processing.


title: ID 190322-1_mock_d43_Bulk


title: ID 210527-13_RAG1_d26_DN2


title: ID 210527-14_RAG1_d26_DN2


title: ID 210527-3_mock_d26_DN2


title: ID 210527-16_LMO2_d26_DN2


title: cDNA_t0

city: Burjassot

first: Jose

middle: E.

last: Perez-Ortin

state: Valencia

address: Dr. Moliner 50

department: Bioquimica y Biologia Molecular

country: Spain

institute: Universitat de Valencia

description: cDNA labelling for t0 sample. Replicates 1-2

hyb_protocol: Hybridization Solution was: 0.5M Na-Phosphate buffer, 1mM EDTA and 7% SDS, pH 7.2. The hybridization protocol used was as follows. Filters were inserted in 12.5X 2.5-cm flat-bottom plastic tubes and pre-hybridized in a rotator oven with 5 ml pre-hybridization solution (the same as used for hybridization but without the radioactive sample) at 65ºC. The pre-hybridization solution was then replaced with 5 ml of the same solution containing 1-5X10exp6 dpm/mL of radioactive sample and hybridized for 48h. Washing conditions were: 20 min at 65ºC in 1X SSC, 0.5% SDS, and twice at 65ºC for 10 min in 0.5X SSC, 0.1% SDS. After quantification, filters were stripped by washing them by pouring with boiling stripping buffer (5 mM sodium phosphate, pH 7.5, 0.1% SDS) over the membrane and left to cool at room temperature. To ensure that radioactivity had been eliminated, the filters were either checked with a Geiger counter.

scan_protocol: Images were acquired using a Fujifilm FLA3000 Phosphorimager. After the washing step, membranes were kept humid, sealed in Saran wrap, avoiding any bubbles, and exposed to an imaging plate (BAS-MP, FujiFilm) for variable times.

data_processing: Raw image quantization with background subtracted for each of the two samples and averaged. The average processed value for each gene was corrected by percentage of guanines (RA data) or uracils (SR data) present in each probe-coding strand as described in García-Martinez et al. 2004.

organism: Saccharomyces cerevisiae

source_name: time 0, before Auxin addition

label_protocol: For GRO samples: In vivo labeling by run-on (GRO) was done using 33P-UTP. Around 6 x 10exp8 yeast cells were used to perform in vivo transcription. After spinning down cells, they were frozen in liquid nitrogen. Samples were then permeabilized with 1 mL of 0.5% sarkosyl and cells were recovered by low-speed centrifugation and the supernatant was removed. In vivo transcription was performed by resuspending cells in 115 µl of RNA water and adding 162 µL of Run On Mix (120 µl of 2.5x transcription buffer [50 mM Tris-HCl pH 7.7, 500 mM KCl, 80 mM MgCl2], 20 µl of AGC mix [10 mM each of CTP, ATP and GTP], 6 µl of DTT [0.1 M] and 13 µl of [α-33P]UTP [3000 Ci/mmol, 10 Ci/µL]). The mix was incubated for 5 minutes at 30ºC to allow transcription elongation. The reaction was stopped by adding 1 ml of cold distilled water to the mix. For mRNA samples: cDNA was synthetized by reverse transcription of total mRNA using oligo d(T)15VN as primer and 33P-dCTP for labelling.

growth_protocol: Cells were grown in YPD at 30ºC for at least 15 hours to reach early exponential phase at 0.4-0.5 OD600. Then, an aliquot of 50 mL of cells were spun-down and collected for t0 sample. Auxin was added to a final concentration of 0.5 mM and two addtional aliquots were taken after 20 and 60 minutes.  The experiment was done in duplicate.

extract_protocol: Total RNA isolated from yeast cells was prepared as described by Sherman et al. [1986], but using a multiple-sample automated device (Fast-Prep, BIO101) to break the cells. Sherman F, Fink GR, Hicks JB (1986) Methods in yeast genetics. Spring Harbor Cold Laboratory Press, Cold Spring Harbor, New York.


title: cDNA_t20

description: cDNA labelling for t20 sample. Replicates 1-2

source_name: time 20 min after Auxin addition


title: cDNA_t60

description: cDNA labelling for t60 sample. Replicates 1-2

source_name: time 60 min after Auxin addition


title: Cme(z)-1, rep1

city: Ikoma

first: Tetsuya

last: Hisanaga

state: Nara

address: Takayama 8916-5

country: Japan

institute: Nara Institute of Science and Technology

data_processing:
Reads were trimmed with Trim Galore with options --stringency 1 --length 5 --paired.
Trimmed reads were aligned to a reference genome using STAR with options --winAnchorMultimapNmax 100 --outFilterMultimapNmax 100 --outFilterMismatchNoverReadLmax 0.04 --outFilterMismatchNoverLmax 1.
Read counts were calculated using TELocal with option --stranded no.
Assembly: C. merolae 10D genome (ASM9120v1)
Supplementary files format and content: Tab-delimited text files include estimated count values for each Sample.

molecule: polyA RNA

organism: Cyanidioschyzon merolae strain 10D

source_name: C. merolae, Cme(z) mutant

growth_protocol: Cyanidioschyzon merolae 10D and Cme(z) cells were grown in liquid culture in sterile modified 2x concentrated Allen´s medium (MA2) at 2.5 < pH < 3.0 under constant white light (80 µmol/m2/s) at 42°C. Cultures were kept in 50 ml falcon tubes aerated with ambient air supplied through a 1ml serological milk pipette coupled to an aquarium pump. No additional CO2 was supplied.

extract_protocol:
RNA was isolated from frozen pellets using the INNUprep RNA Kit (Analytik Jena) according to the manufacturer's instructions with the RL buffer supplemented with 10µl/ml beta-mercaptoethanol (β-ME).
mRNA library was prepared using polyA enrichment and the NEBnext Ultra RNA Library Prep Kit producing unstranded data.

tag: genotype


title: GRO_t60

description: GRO labelling for t60 sample. Replicates 1-2


title: ID 210527-5_EFS_d26_DN2


title: ID 210527-7_RSF91_d26_DN2


title: Cme(z)-1, rep3


title: GRO_t0

description: GRO labelling for t0 sample. Replicates 1-2


title: ID 210527-1_mock_d26_DN2


title: ID 210527-10_RAG2_d26_DN2


title: ID 210527-2_mock_d26_DN2


title: ID 210527-6_EFS_d26_DN2


title: Cme(z)-1, rep2


title: ID 200131-9_RSF91_d26_DN2


title: wild type 10D, rep3

source_name: C. merolae, WT

value: wild type


title: GRO_t20

description: GRO labelling for t20 sample. Replicates 1-2


title: ID 200131-12_LMO2_d26_DN2


title: ID 200131-6_EFS_d26_DN2


title: wild type 10D, rep2


title: wild type 10D, rep1


title: ID 200131-8_RSF91_d26_DN2


title: ID 200131-3_mock_d26_DN2


title: Mpmet, 2 weeks thalli, T-WGBS

data_processing:
Sequencing data were analyzed using the  nf-core/methylseq pipeline to generate cytosine methylation reports
Each cytosine which is covered by at least six reads was used for further analyses. Methylation ratio of each cytosine was calculated and summarized to a bed file.
Assembly: https://marchantia.info/download/MpTak1_v5.1r2/
Supplementary files format and content: Cytosine methylation reports
Supplementary files format and content: bedgraph files for methylation ratio of each cytosine per each context

organism: Marchantia polymorpha

source_name: 2 weeks old thalli

growth_protocol: Plants were cultured on 0.5 Gamborg’s B5 medium solidified with 1% agar under continuous white light at 22°C.

extract_protocol:
Genomic DNA was extracted from 100 mg of 4 weeks old thallus tissue of M. polymorpha using Nucleon PhytoPure (cytiva). A tagmentation-based approach was used as in (Wang et al., 2013).  Bisulfite treatment was performed with a EZ DNA Methylation-Gold kit (Zymo Research). Sequencing libraries were amplified by using KAPA 2G robust HS Master Mix (Sigma-Aldrich, Merck KGaA). These libraries were sequenced on a Illumina NextSeq 2000 to generate 50 bp paired end reads.
T-WGBS

value: 2 weeks old thalli

tag: cell line

value: Mpmet


title: Tak-1 wild type, 2 weeks thalli, T-WGBS


title: ID 200131-11_LMO2_d26_DN2


title: exp set3, Mpmet-3 14d thalli, biol rep3

data_processing:
Reads were trimmed with Trim Galore with options --stringency 1  --length 5 --paired
Trimmed reads were aligned to a reference genome using STAR with options --winAnchorMultimapNmax 100 --outFilterMultimapNmax 100  --outFilterMismatchNoverReadLmax 0.04 --outFilterMismatchNoverLmax 1
Read counts were calculated using TELocal with option --stranded reverse.
Assembly: https://marchantia.info/download/MpTak1_v5.1r2/
Supplementary files format and content: tab-delimited text files include estimated count values for each Sample and for each strand

source_name: 14d thalli

growth_protocol: Plants were cultured on half-strength Gamborg’s B5 medium solidified with 1% (w/v) agar under continuous white light at 22°C

extract_protocol:
Total RNA was extracted using a Spectrum Plant Total RNA kit (Sigma Aldrich),  1 ug of total RNA was used for the construction of sequencing libraries.
PolyA RNA was selected using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs), then RNA-seq libraries were generated  using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs)

value: 14d thalli


title: ID 200131-5_EFS_d26_DN2


title: exp set3, Mpmet-3 14d thalli, biol rep2


title: exp set3, Mpmet-3 14d thalli, biol rep1


title: ID 210527-9_RSF91_d26_DN2


title: ID 200131-2_mock_d26_DN2


title: ID 200131-4_EFS_d26_DN2


title: ID 200131-7_RSF91_d26_DN2


title: ID 200131-10_LMO2_d26_DN2


title: exp set3, Tak-1 14d thalli, biol rep3

value: Tak-1 wild type


title: exp set3, Tak-1 14d thalli, biol rep2


title: ID 200131-1_mock_d26_DN2


title: ID 190906-11_LMO2_d26_DN2


title: exp set2, Mpknox2-1 Mpez1-2, 14d thalli, biol rep3

data_processing:
Reads were trimmed with Trim Galore with options --stringency 1 --length 5 --paired.
Trimmed reads were aligned to a reference genome using STAR with options --winAnchorMultimapNmax 100 --outFilterMultimapNmax 100 --outFilterMismatchNoverReadLmax 0.04 --outFilterMismatchNoverLmax 1.
Read counts were calculated using TELocal with option --stranded reverse. Gene annotation
Assembly: https://marchantia.info/download/MpTak1_v5.1r2/
Supplementary files format and content: Tab-delimited text files include estimated count values for each Sample and for each strand.

growth_protocol: Plants were cultured on half-strength Gamborg’s B5 medium solidified with 1% (w/v) agar under continuous white light at 22°C.

extract_protocol:
Total RNA was extracted using a Spectrum Plant Total RNA kit (Sigma Aldrich), 1 ug of total RNA was used for the construction of sequencing libraries.
PolyA RNA was selected using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs), then RNA-seq libraries were generated using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs).

value: Mpknox2-1 Mpez1-2


title: exp set2, Mpknox2-1 Mpez1-2, 14d thalli, biol rep2


title: exp set2, Mpknox2-1 Mpez1-2, 14d thalli, biol rep1


title: exp set2, Mpknox2-1, 14d thalli, biol rep3


title: exp set2, Mpknox2-1, 14d thalli, biol rep2


title: exp set2, Mpknox2-1, 14d thalli, biol rep1


title: exp set3, Tak-1 14d thalli, biol rep1


title: ID 190906-10_LMO2_d26_DN2


title: ID 190906-8_RSF91_d26_DN2


title: ID 190906-9_RSF91_d26_DN2


title: ID 190906-7_RSF91_d26_DN2


title: exp set1, Mpknox2-1 Mpez1-1, 14d thalli, biol rep3

value: Mpknox2-1 Mpez1-1


title: ID 190906-2_mock_d26_DN2


title: ID 190726-12_LMO2_d26_DN2


title: exp set1, Mpknox2-1 Mpez1-1, 14d thalli, biol rep2


title: ID 190726-7_RSF91_d26_DN2


title: ID 190726-2_mock_d26_DN2


title: ID 190726-3_mock_d26_DN2


title: ID 190726-11_LMO2_d26_DN2


title: exp set1, Mpknox2-1, 14d thalli, biol rep1


title: exp set1, Mpknox2-1, 14d thalli, biol rep2


title: exp set1, Mpknox2-1, 14d thalli, biol rep3


title: ID 190726-6_RSF91_d26_DN2


title: WM1 cells, with 50 mg/L tetracyline addition,rep2

city: Beijing

first: Xiuli

last: Chen

state: Beijing

address: 9/5000  School of Environmental Science

country: China

institute: Peking University

data_processing:
Mapping reads to reference genome (Bowtie2)
rRNA contamination assessment (Blast)
Expression analysis (RSEM)
Differential expression analysis (DESeq2)
Assembly: NCBI accession number CP120709
Supplementary files format and content: Tab-delimited text file includes TPM values of each sample

organism: Sphingobacterium

extract_protocol:
Total RNA was extracted from the collected cells using TRIzol® Reagent (Invitrogen) and further purified using DNase I (TaKara).
RNA-seq transcriptome library was prepared following TruSeqTM RNA sample preparation Kit from Illumina (San Diego, CA) using 2μg of total RNA.

value: with 50 mg/L tetracyline addition


title: WM1 cells, with 50 mg/L tetracyline addition,rep3


title: ID 190726-9_LMO2_d26_DN2


title: ID 190726-8_RSF91_d26_DN2


title: WM1 cells, with 50 mg/L tetracyline addition,rep1


title: ID 190726-1_mock_d26_DN2


title: ID 190614-6_RSF91_d26_DN2


title: ID 190614-7_RSF91_d26_DN2


title: ID 190614-4_mock_d26_DN2


title: exp set1, Mpknox2-1 Mpez1-1, 14d thalli, biol rep1


title: WM1 cells, without tetracyline addition,rep3

value: without tetracyline addition


title: ID 190726-5_RSF91_d26_DN2


title: WM1 cells, without tetracyline addition,rep2


title: WM1 cells, without tetracyline addition,rep1


title: ID 190614-9_LMO2_d26_DN2


title: JHH-6 cells transiently transfected with 100 pmol siRNA APE1, replicate 2

city: Trieste

first: Silvano

last: Piazza

address: localita' Padriciano 99

country: Italy

institute: ICGEB

data_processing: Transcript counts were normalized through the normalization method incorporated in the model framework, estimating parameters from positive controls, negative controls, and housekeeping genes embedded in the nCounter system, using the NanoStringDiff package within Bioconductor. The quality of the data was checked by the mean expression and SD values for housekeeping miRNAs and positive/negative controls. Differential expression of genes was assessed on log2-normalized data with a generalized linear model likelihood ratio test, using the glm.LRT function within the NanoStringDiff package. A padj cutoff of 0.1 was used to determine statistical significance.

organism: Homo sapiens

source_name: JHH-6 hepatocellular carcinoma cell line

label_protocol: Samples were prepared for nCounter miRNA expression profiling using the human v3 miRNA expression panel, according to the manufacturer’s recommendations (NanoString, Seattle, Washington, USA) for hybridization, detection, and scanning.

growth_protocol: JHH-6 hepatocarcinoma cells were grown in William’s medium E (Sigma-Aldrich, St. Louis, MO) and supplemented with 10% fetal bovine serum (Euroclone), 2 mM L-glutamine (Euroclone), 100 U/mL penicillin and 100 mg/mL streptomycin. Cells were negative for mycoplasma. The tests were performed with N-GARDE Mycoplasma PCR Reagent (Euroclone).

extract_protocol: RNA extraction was carried out using miRNeasy kit (Qiagen, USA) following the manufacturer’s protocols.

treatment_protocol: JHH-6 cells were seeded in the number of  1.0*10^6 in 10 cm dish. One day later, the transfection was performed using 100 pmol of custom hAPE1 siRNA or with non-targeting siRNA pool (siSCR) as a control, (GE Healthcare Dharmacon, Colorado, USA). The DharmaFECT transfection reagent (Dharmacon) was employed for transfection following to the manufacturer’s protocols. Two days after transfection, cells were collected and whole cell extract and RNA were prepared.


title: ID 190322-9_LMO2_d25_DN2


title: JHH-6 cells transiently transfected with 100 pmol siRNA APE1, replicate 1


title: JHH-6 cells transiently transfected with 100 pmol scramble control siRNA, replicate 2

value: control


title: k47_ko_3

city: Stockholm

first: Eike-Benjamin

last: Braune

address: Nobels väg 6

department: CMB

country: Sweden

institute: Karolinska Institutet

data_processing:
Clustering was done by 'cBot' and samples were sequenced on HiSeq2500 (HiSeq Control Software 2.2.58/RTA 1.18.64) with a 1x51 setup using 'HiSeq SBS Kit v4' chemistry. The Bcl to FastQ conversion was performed using None from the CASAVA software suite. The quality scale used is Sanger / phred33 / Illumina 1.8+.
Data have been processed following the RNA-seq pipeline described in : Muhl, L., Genové, G., Leptidis, S. et al. Single-cell analysis uncovers fibroblast heterogeneity and criteria for fibroblast and mural cell identification and discrimination. Nat Commun 11, 3953 (2020). https://doi.org/10.1038/s41467-020-17740-1
Assembly: mus musculus, mm10
Supplementary files format and content: read count file , count.txt

source_name: heart

extract_protocol:
Metallic beads are added to the mixture and TissueLyzer (Qiagen) was used with the following setting: 50Hz for 2 minutes twice with a fast spin in between.  0.1 ml of chloroform per 0.5 ml of Trizol was added, samples were shaken vigorously for 15 sec and kept for 10 minutes at RT and then centrifuged for 5 minutes at 12,000 x g. The transparent aqueous phase was then transferred to a fresh tube. The RNA was precipitated with 250 l of isopropanol per 0.5 ml of Trizol used for the initial homogenization. Samples were kept on ice for 1 hour and centrifuged for 10 minutes at 4 °C at 12,000 x g. The pellet was washed once with 75 % ethanol by flipping the tube without disturbing the pellet and subsequently centrifuged for 10 minutes at 12,000 x g (4 °C). The ethanol was removed, and the pellet resuspended in 50 l of RNAse and DNAse free water (Ambion).
The RNA was quantified with Qbit (Invitrogen) and loaded in a 384 well plate for subsequent cDNA conversion using a previously described protocol 60. Briefly, the RNA undergoes a first-strand conversion in a reaction mixture with SuperScript II reverse transcriptase (Invitrogen), RNase inhibitor (Takara), 5X SuperScript II First-Strand buffer (Invitrogen), DTT (Invitrogen), 5M Betaine (Sigma) MgCl2 (Sigma), and custom locked nucleic acid oligonucleotides. Reverse transcription was performed for 10 cycles followed by inactivation at 70°C for 15 minutes. The total volume of cDNA was added into PCR master mix containing KAPA HiFi HotStart ReadyMix (KAPA Biosystems) and ISPCR primers. The PCR reaction was performed for 22 cycles. Next, PCR products were purified using SeraMag beads (Sigma Aldrich) and 17% PEG, with the final elution in EB buffer (Qiagen). The library quality and size distribution were checked using a High-Sensitivity DNA chip (Agilent Bioanalyzer). For the library preparation, cDNA samples were mixed with 10mM TAPS-NaOH, 5mM MgCl2 pH 8,5, 10% PEG 8K, Tn5 enzyme and water and incubated at 55°C for 6 minutes. After this step the samples were incubated with 0,2% SDS for 5 minutes at RT. Then the samples were pooled after enrichment PCR as described by the manufacturer (illumina) through the use of a dual-index strategy, referred to as index 1 (i7) and index 2 (i5). The sequencing was then performed using a HiSeq 3000 unit at the Single Cell Core Facility (SICOF) at Karolinska Institute (Sweden).

treatment_protocol: Mice were euthanized, the organs removed and immediately fresh frozen. Frozen tissue from the left ventricle was cut on ice and homogenized in 0.5 ml Trizol (Thermo Fisher Scientific) per 25 mg tissue.

value: heart

value: Notch3 -/- (N3 KO)

tag: technical replicate


title: k47_ko_2


title: k47_ko_1


title: k56_ko_3


title: ID 190322-1_mock_d25_DN2


title: JHH-6 cells transiently transfected with 100 pmol scramble control siRNA, replicate 3


title: ID 190322-7_RSF91_d25_DN2


title: ID 190322-5_RSF91_d25_DN2


title: ID 190322-3_mock_d25_DN2


title: ID 190322-11_LMO2_d25_DN2


title: k56_ko_2


title: JHH-6 cells transiently transfected with 100 pmol scramble control siRNA, replicate 1


title: JHH-6 cells transiently transfected with 100 pmol siRNA APE1, replicate 3


title: k54_ko_3


title: k56_ko_1


title: k54_ko_2


title: Patient 2

city: Paris

first: Nassima

last: Bekaddour

address: 45 rue des Saints-Pères

country: France

institute: Université Paris Cité

hyb_protocol: The extracted RNA was diluted to a concentration of 20 ng/μl using ribonuclease-free water. Subsequently, 100 ng (5 μl) of each sample was subjected to analysis utilizing the Human  Immunology kit v2 and Nanostring Counter. T

scan_protocol: To ensure accuracy, each sample underwent individual multiplexed reactions, consisting of eight negative probes and six serial concentrations of positive control probes.

data_processing:
The resulting data was then imported into the nSolver analysis software (version 2.5) for quality assessment and data normalization, adhering to NanoString analysis guidelines. This normalization process involved the utilization of positive probes and housekeeping genes.
Before conducting hierarchical clustering via Qlucore Omics Explorer version 3.1, the mRNA expression levels underwent logarithmic transformation. In terms of pathway analysis, we utilized the DAVID bioinformatics database (https://david.ncifcrf.gov). Supplementary data encompasses the displayed mRNA levels

label: no label

source_name: Isolated Monocytes from Human Blood

label_protocol: no label

extract_protocol: Isolated monocytes from both control subjects and patients were used to extract total RNA using the Thermoscientific GeneJET RNA Purification Kit.

treatment_protocol: No treatment was administered. The aim here is to compare monocytes from healthy donors and AJI patients.

value: Isolated Monocytes from Human Blood

value: The cells utilized in this study are freshly obtained (not frozen)

tag: subject status

value: OligoAJI Patient

tag: gender


title: Patient 3

value: female


title: Patient 1


title: k54_ko_1


title: Rnaseh 1-KO-P-3

city: Hangzhou

first: Heng-yu

last: Fan

state: Zhejiang

address: Yuhangtang Road

institute: Life Sciences  Institute

data_processing:
RNA-Seq was performed with biological replicates for all samples.
Raw reads were trimmed to 50 bp and mapped to the mouse genome (mm9) and ERCC spike-in sequences with Tophat v2.1.1.
Only uniquely mapped reads were subsequently assembled into transcripts guided by the reference annotation (UCSC gene models) with Cufflinks v2.2.1.
Expression level of each gene was quantified with normalized FPKM (fragments per kilobase of exon per million mapped fragments).
Expression level  was further normalized with the ERCC spike-in.
Genome_build: mm9
Supplementary_files_format_and_content: Tab-delimited text files include FPKM values of all RNA-seq samples

source_name: Pachytene

extract_protocol:
Leptotene/zygotene and pachytene spermatogenic cells were collected with Fluorescence activated Cell Sorting (FACS) with purity verification.
Sequencing libraries were constructed from 500 pg of amplified cDNA using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme, TD503) according to manufacturer’s instructions. Barcoded libraries were pooled and sequenced on the Illumina HiSeq X Ten platform with 150 bp paired-end reads

tag: strain background

tag: genotype/variation

value: Rnaseh1 knock-out

value: Spermatocyte

tag: developmental stage

value: Pachytene


title: Healthy Donor 3

value: Healthy donors sourced from the French Blood Establishment, ensuring their anonymity.


title: k71_wt_2

tag: tissue

value: Notch3 +/+ (WT)


title: k71_wt_3


title: Rnaseh 1-KO-P-2


title: k71_wt_1


title: k61_wt_2


title: k61_wt_3


title: Rnaseh 1-KO-P-1


title: k61_wt_1


title: WT-P-3

value: WT


title: Healthy Donor 1


title: WT-P-2


title: WT-P-1


title: Rnaseh 1-KO-LZ-2

source_name: Leptotene/zygotene

value: Leptotene/zygotene


title: Rnaseh 1-KO-LZ-3


title: X557_wt_3


title: X557_wt_1


title: X553_wt_3


title: X557_wt_2


title: Healthy Donor 2


title: Rnaseh 1-KO-LZ-1


title: X553_wt_2


title: WT-LZ-3


title: WT-LZ-2


title: X553_wt_1


title: HC_2，replicate 2, scRNAseq

city: Hefei

first: Yin

last: Zhang

state: Anhui

address: No. 218 Jixi Rd

institute: First Affiliated Hospital of Anhui Medical University

description: Singleron

library_source: transcriptomic single cell

data_processing:
Celescope was used to base-calling, alignment, filtering and generation of normalized abundance measurements.
Read 1: contains the 24bp GEXSCOPE barcode and the 12bp UMI (bases 25-36). Read 2 contains the cDNA information.
Assembly: GRCh38
Supplementary files format and content: matrix files

source_name: Peripheral blood

extract_protocol: We used GEXSCOPETM Single Cell RNA Library Kit Tissue (Singleron Biotechnologies) to barcode single cells, capture mRNA from isolated single cells, and generate cDNA libraries for scRNA-seq. Then, individual libraries were diluted to 4 ng/μl, and pooled for sequencing with 150 bp paired-end reads.

value: Peripheral blood

value: PBMC

tag: age

value: male


title: WT-LZ-1


title: HC_1，replicate 1, scRNAseq

description: Singleron


title: PHC_2，replicate 2, scRNAseq

description: Singleron


title: PHC_1，replicate 1, scRNAseq

description: Singleron


title: PI_3，replicate 3, scRNAseq

description: Singleron


title: PI_1，replicate 1, scRNAseq

description: Singleron


title: let-418, embryo, 26°C, RNA-seq rep1

first: Zijie

last: Shen

address: singhua University, Haidian District

department: School of Life Science

institute: Tsinghua University

data_processing:
The raw sequencing reads were trimmed using Trim_galore (version 0.4.4) to remove the low-quality bases and adaptor sequences
Paired-end reads with at least 20 nucleotides long were aligned to C. elegans reference genome (ce11) using STAR (2.5.4b).
The numbers of reads that aligned to genes were quantified by HTSeq (version 0.9.1).
Assembly: ce11
Supplementary files format and content: tab-delimited text file includes raw counts for each sample

organism: Caenorhabditis elegans

source_name: embryo

extract_protocol:
embryos were lysed in 400μL TRIzol reagent (Invitrogen). Total RNA was extracted according to the manufacturer’s protocol.
library was constructed using KAPA RNA HyperPrep Kit (KAPA Biosystems), according to the manufacturer's protocol.

value: embryo

tag: treatment


title: let-418, embryo, 20°C, RNA-seq rep2


title: let-418, embryo, 26°C, RNA-seq rep2


title: IA_2，replicate 2, scRNAseq

description: Singleron

tag: Sex


title: let-418, embryo, 20°C, RNA-seq rep1


title: IA_1，replicate 1, scRNAseq

description: Singleron


title: WT, embryo, 26°C, RNA-seq rep2

value: wild-type


title: WT, embryo, 26°C, RNA-seq rep1


title: IC_2，replicate 2, scRNAseq

description: Singleron


title: N2, input

description: ATAC-seq

data_processing:
The raw sequencing reads were trimmed using Trim_galore (version 0.4.4) to remove the low-quality bases and adaptor sequences.
Paired-end reads with at least 20 nucleotides long were aligned to C. elegans reference genome (ce11) using bwa(version 0.7.17).
macs2(version 2.1.1) was used to call peaks of short DNA fragments.
Assembly: ce11
Supplementary files format and content: bed (except for input sample)
Supplementary files format and content: narrowPeak (except for input sample)

extract_protocol:
The live embryos are incubated in chitinase solution and slowly rotated at room temperature for 0.5-1 hours to digest egg shells. embryos were dissociated into single cells by pipetting up and down and resuspended in ATAC lysis buffer incubated at 4℃ for 10 minutes to break cell membrane and release nuclei.
Nuclei were used for library preparation directly by Vazyme TruePrep DNA Library Kit (Vazyme), according to the manufacturer's protocol.

value: input


title: let-418+nurf-1, embryo, 26°C, ATAC-seq rep1

description: ATAC-seq


title: IC_1，replicate 1, scRNAseq

description: Singleron


title: let-418, embryo, 20°C, ATAC-seq rep1

description: ATAC-seq


title: let-418, embryo, 26°C, ATAC-seq rep2

description: ATAC-seq


title: let-418, embryo, 20°C, ATAC-seq rep2

description: ATAC-seq


title: WT, embryo, 26°C, ATAC-seq rep1

description: ATAC-seq


title: WT, embryo, 26°C, ATAC-seq rep2

description: ATAC-seq


title: let-418, embryo, 26°C, ATAC-seq rep1

description: ATAC-seq


title: PI_2，replicate 2, scRNAseq

description: Singleron


title: let-418+nurf-1, embryo, 26°C, ATAC-seq rep2

description: ATAC-seq


title: MUC22830 CM-OXT-202-24-K

city: Neuherberg

first: Dominik

last: Lutter

address: Ingolstädter Landstraße 1

department: Institute for Diabetes and Obesity

institute: Helmholtz Zentrum München

data_processing:
Technical replicates of the samples were merged, and reads were then mapped to rRNA (from Ensembl, GRCm38 release 102) using Bowtie2 (version 2.3.4.3114) and subsequently, unmapped reads were mapped to the mm10 genome (from Ensembl, GRCm38 release 102) using STAR (2.7.0115). Count matrices were generated by counting reads corresponding to gene exons using featureCounts (version 1.6.3116). Counts were transformed to FPKM and TPM using the actual feature length. The single-nuclei transcriptomics analysis of raw count matrix loaded into python and stored as AnnData object was performed using Scanpy version 1.7.1. Filtering of the cells was based on a minimum count number of 250. Filtering was applied to the matrix of 1536 single nuclei and 55579 genes. Nuclei that had >1000 and <4500 detected genes were kept. Genes detected in fewer than 25 cells and with read count below 250 were filtered out. Hence, only the nuclei having less than 4500 genes detected and a library size between 10000 and 200000 reads were kept. Applying this filtering strategy, the final matrix comprises 1202 single nuclei and 13867 genes. Batch effect corrections were performed employing combat using plates as a covariate.
Assembly: mm10
Supplementary files format and content: Tab-separated values files and matrix files

molecule: nuclear RNA

source_name: Brain

extract_protocol:
The single-nucleus RNA-seq2 methodology was used to capture a high number of transcripts from frozen tissues. the reaction volumes were miniaturized with the aid of the Mosquito HV robot. Per well, 2190 nL of Lysis Buffer 2 (LB2) were dispensed. The final volume of the mixture of Lysis Buffer (LB1 and LB2) was 3.125 μL, containing NP40 2%, Triton-X100 1%, 1/300,000 diluted ERCC RNA spike-in, 3′ SMART-seq CDS Primer II A and RNAse-free water. ERCC spike-ins (Thermo Fischer Scientific, Ref. 4456740; Lot no. 00892098) were diluted 1 in 10, with RNAse-free water with 0.4 U/μL Recombinant RNase Inhibitor (Takara Clontech, Ref. 2313A) and a fresh dilution of 1 in 300,000 was prepared before the first strand synthesis. Reverse transcription and Pre-PCR amplification steps were followed as described by the manufacturer with four-times reduced volumes for all steps.
Sequencing libraries were prepared using the Illumina Nextera XT DNA Sample Preparation kit (Illumina, Ref. FC-131-1096) and the combination of 384 Combinatorial Dual Indexes (Illumina- Set A to D, Ref. FC-131-2001 to FC-131-2004). In brief, 500 nL of the undiluted cDNA were transferred to a new 384 well-plate containing 1500 nL of Tagmentation Mix (TD and ATM reagents). Accordingly, all Nextera XT reagents (NT, NPM and i5/i7 indexes) were added stepwise to a final library volume of 5 μL per well. The final PCR amplification was performed through 12 cycles. Once the libraries were prepared, 500 nL from each well were pooled together into a tube (total volume of ~192 μL) to perform a final AMPure XP bead (Beckman Coulter, Ref. A63882) clean-up step. For our single nuclei libraries, two consecutive clean-ups with a ratio of sample to bead 0,9X led to library sizes between 200 and 1000 bp. Each plate, counting with a total of 384 libraries, was pooled together into one final library. A total of 4 final libraries were sequenced using an Illumina NovaSeq 6000 NGS sequencer in an SP XP flowcell, in a paired-end 150 bases length.

tag: cell type

value: Oxytocin neurons

value: Brain

value: C57BL/6JRj -OXT-sun1-sfGFP

value: 6 months

value: Male

tag: treatment/diet

value: HFHS diet


title: MUC22835 CM-OXT-202-24-P


title: MUC22829 CM-OXT-202-24-J


title: MUC22833 CM-OXT-202-24-N


title: MUC22834 CM-OXT-202-24-O


title: MUC22832 CM-OXT-202-24-M


title: MUC22826 CM-OXT-202-24-G

value: SC diet


title: MUC22825 CM-OXT-202-24-F


title: MUC22822 CM-OXT-202-24-C


title: MUC22828 CM-OXT-202-24-I


title: MUC22823 CM-OXT-202-24-D


title: MUC22827 CM-OXT-202-24-H


title: MUC22819 CM-OXT-202-23-P


title: MUC22821 CM-OXT-202-24-B


title: MUC22831 CM-OXT-202-24-L


title: MUC22820 CM-OXT-202-24-A


title: MUC22824 CM-OXT-202-24-E


title: MUC22817 CM-OXT-202-23-N


title: MUC22818 CM-OXT-202-23-O


title: MUC22816 CM-OXT-202-23-M


title: MUC22815 CM-OXT-202-23-L


title: MUC22814 CM-OXT-202-23-K


title: MUC22813 CM-OXT-202-23-J


title: MUC22804 CM-OXT-202-23-A


title: MUC22794 CM-OXT-202-22-G


title: MUC22811 CM-OXT-202-23-H


title: MUC22801 CM-OXT-202-22-N


title: MUC22795 CM-OXT-202-22-H


title: MUC22806 CM-OXT-202-23-C


title: MUC22803 CM-OXT-202-22-P


title: MUC22807 CM-OXT-202-23-D


title: MUC22812 CM-OXT-202-23-I


title: MUC22808 CM-OXT-202-23-E


title: MUC22799 CM-OXT-202-22-L


title: MUC22809 CM-OXT-202-23-F


title: MUC22810 CM-OXT-202-23-G


title: MUC22800 CM-OXT-202-22-M


title: MUC22802 CM-OXT-202-22-O


title: MUC22793 CM-OXT-202-22-F


title: MUC22797 CM-OXT-202-22-J


title: MUC22798 CM-OXT-202-22-K


title: MUC22796 CM-OXT-202-22-I


title: MUC22805 CM-OXT-202-23-B


title: MUC22792 CM-OXT-202-22-E


title: MUC22789 CM-OXT-202-22-B


title: MUC22791 CM-OXT-202-22-D


title: MUC22790 CM-OXT-202-22-C


title: MUC22778 CM-OXT-202-21-G


title: MUC22788 CM-OXT-202-22-A


title: MUC22781 CM-OXT-202-21-J


title: MUC22783 CM-OXT-202-21-L


title: MUC22777 CM-OXT-202-21-F


title: MUC22776 CM-OXT-202-21-E


title: MUC22787 CM-OXT-202-21-P


title: MUC22785 CM-OXT-202-21-N


title: MUC22782 CM-OXT-202-21-K


title: MUC22784 CM-OXT-202-21-M


title: MUC22786 CM-OXT-202-21-O


title: MUC22775 CM-OXT-202-21-D


title: MUC22774 CM-OXT-202-21-C


title: MUC22773 CM-OXT-202-21-B


title: MUC22772 CM-OXT-202-21-A


title: MUC22771 CM-OXT-202-20-P


title: MUC22780 CM-OXT-202-21-I


title: MUC22779 CM-OXT-202-21-H


title: MUC22769 CM-OXT-202-20-N


title: MUC22770 CM-OXT-202-20-O


title: MUC22768 CM-OXT-202-20-M


title: MUC22765 CM-OXT-202-20-J


title: MUC22762 CM-OXT-202-20-G


title: MUC22767 CM-OXT-202-20-L


title: MUC22763 CM-OXT-202-20-H


title: MUC22761 CM-OXT-202-20-F


title: MUC22764 CM-OXT-202-20-I


title: MUC22757 CM-OXT-202-20-B


title: MUC22758 CM-OXT-202-20-C


title: MUC22759 CM-OXT-202-20-D


title: MUC22760 CM-OXT-202-20-E


title: MUC22766 CM-OXT-202-20-K


title: MUC22756 CM-OXT-202-20-A


title: MUC22754 CM-OXT-202-19-O


title: MUC22753 CM-OXT-202-19-N


title: MUC22755 CM-OXT-202-19-P


title: MUC22752 CM-OXT-202-19-M


title: MUC22751 CM-OXT-202-19-L


title: MUC22750 CM-OXT-202-19-K


title: MUC22749 CM-OXT-202-19-J


title: MUC22747 CM-OXT-202-19-H


title: MUC22745 CM-OXT-202-19-F


title: MUC22742 CM-OXT-202-19-C


title: MUC22746 CM-OXT-202-19-G


title: MUC22744 CM-OXT-202-19-E


title: MUC22740 CM-OXT-202-19-A


title: MUC22739 CM-OXT-202-18-P


title: MUC22748 CM-OXT-202-19-I


title: MUC22738 CM-OXT-202-18-O


title: MUC22737 CM-OXT-202-18-N


title: MUC22743 CM-OXT-202-19-D


title: 07A0221.3.1

type: genomic

city: Badalona

first: Manel

last: Esteller

state: Barcelona

address: Ctra. de Can Ruti, Cami de les Escoles s/n

department: Cancer and Leukemia Epigenetics and Biology Program (PEBCL)

institute: Josep Carreras Leukaemia Research Institute (IJC)

hyb_protocol: Standard Illumina Protocol

scan_protocol: Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting

data_processing: R

label: Cy5 and Cy3

source_name: Lung

label_protocol: Standard Illumina Protocol

extract_protocol: Genomic DNA was extracted and purified from the different samples using Qiagen DNeasy Kit according to standard instructions

value: Lung

tag: condition

value: Healthy


title: MUC22736 CM-OXT-202-18-M

tag: strain


title: MUC22735 CM-OXT-202-18-L


title: MUC22734 CM-OXT-202-18-K


title: MUC22733 CM-OXT-202-18-J


title: MUC22731 CM-OXT-202-18-H


title: MUC22730 CM-OXT-202-18-G


title: MUC22729 CM-OXT-202-18-F


title: MUC22727 CM-OXT-202-18-D


title: MUC22722 CM-OXT-202-17-O


title: MUC22728 CM-OXT-202-18-E


title: MUC22725 CM-OXT-202-18-B


title: MUC22724 CM-OXT-202-18-A


title: 07A0146.4.1


title: MUC22723 CM-OXT-202-17-P


title: MUC22717 CM-OXT-202-17-J


title: MUC22719 CM-OXT-202-17-L


title: 07A0193.3.2


title: MUC22718 CM-OXT-202-17-K


title: MUC22726 CM-OXT-202-18-C


title: MUC22720 CM-OXT-202-17-M


title: 06A022.4.1


title: MUC22716 CM-OXT-202-17-I


title: 07A0148.3.1


title: MUC22732 CM-OXT-202-18-I


title: 07A1189.2.1


title: 07A0227.7.1


title: 07A0167.3.1


title: MUC22714 CM-OXT-202-17-G


title: 07A0173.3.1


title: MUC22712 CM-OXT-202-17-E


title: MUC22711 CM-OXT-202-17-D


title: MUC22715 CM-OXT-202-17-H


title: MUC22707 CM-OXT-202-16-P


title: MUC22706 CM-OXT-202-16-O


title: MUC22709 CM-OXT-202-17-B


title: MUC22704 CM-OXT-202-16-M


title: MUC22710 CM-OXT-202-17-C


title: MUC22721 CM-OXT-202-17-N


title: MUC22703 CM-OXT-202-16-L


title: MUC22708 CM-OXT-202-17-A


title: MUC22702 CM-OXT-202-16-K


title: MUC22705 CM-OXT-202-16-N


title: MUC22701 CM-OXT-202-16-J


title: MUC22696 CM-OXT-202-16-E


title: MUC22697 CM-OXT-202-16-F


title: MUC22700 CM-OXT-202-16-I


title: MUC22698 CM-OXT-202-16-G


title: MUC22713 CM-OXT-202-17-F


title: MUC22695 CM-OXT-202-16-D


title: MUC22694 CM-OXT-202-16-C


title: MUC22690 CM-OXT-202-15-O


title: MUC22691 CM-OXT-202-15-P


title: MUC22686 CM-OXT-202-15-K


title: MUC22689 CM-OXT-202-15-N


title: MUC22684 CM-OXT-202-15-I


title: MUC22688 CM-OXT-202-15-M


title: MUC22687 CM-OXT-202-15-L


title: MUC22685 CM-OXT-202-15-J


title: MUC22699 CM-OXT-202-16-H


title: MUC22683 CM-OXT-202-15-H


title: MUC22682 CM-OXT-202-15-G


title: MUC22693 CM-OXT-202-16-B


title: MUC22678 CM-OXT-202-15-C


title: MUC22680 CM-OXT-202-15-E


title: MUC22679 CM-OXT-202-15-D


title: MUC22681 CM-OXT-202-15-F


title: MUC22674 CM-OXT-202-14-O


title: MUC22677 CM-OXT-202-15-B


title: MUC22673 CM-OXT-202-14-N


title: MUC22676 CM-OXT-202-15-A


title: MUC22668 CM-OXT-202-14-I


title: MUC22692 CM-OXT-202-16-A


title: MUC22666 CM-OXT-202-14-G


title: MUC22675 CM-OXT-202-14-P


title: MUC22672 CM-OXT-202-14-M


title: MUC22664 CM-OXT-202-14-E


title: MUC22671 CM-OXT-202-14-L


title: MUC22663 CM-OXT-202-14-D


title: MUC22670 CM-OXT-202-14-K


title: MUC22659 CM-OXT-202-13-P


title: MUC22658 CM-OXT-202-13-O


title: MUC22667 CM-OXT-202-14-H


title: MUC22665 CM-OXT-202-14-F


title: MUC22661 CM-OXT-202-14-B


title: MUC22655 CM-OXT-202-13-L


title: MUC22650 CM-OXT-202-13-G


title: MUC22653 CM-OXT-202-13-J


title: MUC22657 CM-OXT-202-13-N


title: MUC22647 CM-OXT-202-13-D


title: MUC22662 CM-OXT-202-14-C


title: MUC22654 CM-OXT-202-13-K


title: MUC22644 CM-OXT-202-13-A


title: MUC22642 CM-OXT-202-12-O


title: MUC22669 CM-OXT-202-14-J


title: MUC22649 CM-OXT-202-13-F


title: MUC22651 CM-OXT-202-13-H


title: MUC22639 CM-OXT-202-12-L


title: MUC22640 CM-OXT-202-12-M


title: MUC22635 CM-OXT-202-12-H


title: MUC22638 CM-OXT-202-12-K


title: MUC22656 CM-OXT-202-13-M


title: MUC22636 CM-OXT-202-12-I


title: MUC22646 CM-OXT-202-13-C


title: MUC22648 CM-OXT-202-13-E


title: MUC22645 CM-OXT-202-13-B


title: MUC22637 CM-OXT-202-12-J


title: MUC22633 CM-OXT-202-12-F


title: MUC22634 CM-OXT-202-12-G


title: MUC22632 CM-OXT-202-12-E


title: MUC22741 CM-OXT-202-19-B


title: MUC22643 CM-OXT-202-12-P


title: MUC22630 CM-OXT-202-12-C


title: MUC22631 CM-OXT-202-12-D


title: MUC22627 CM-OXT-202-11-P


title: MUC22626 CM-OXT-202-11-O


title: MUC22625 CM-OXT-202-11-N


title: MUC22641 CM-OXT-202-12-N


title: MUC22624 CM-OXT-202-11-M


title: MUC22628 CM-OXT-202-12-A


title: MUC22623 CM-OXT-202-11-L


title: MUC22621 CM-OXT-202-11-J


title: MUC22620 CM-OXT-202-11-I


title: MUC22622 CM-OXT-202-11-K


title: MUC22619 CM-OXT-202-11-H


title: MUC22660 CM-OXT-202-14-A


title: MUC22618 CM-OXT-202-11-G


title: MUC22615 CM-OXT-202-11-D


title: MUC22617 CM-OXT-202-11-F


title: MUC22616 CM-OXT-202-11-E


title: MUC22610 CM-OXT-202-10-O


title: MUC22607 CM-OXT-202-10-L


title: MUC22609 CM-OXT-202-10-N


title: MUC22611 CM-OXT-202-10-P


title: MUC22614 CM-OXT-202-11-C


title: MUC22612 CM-OXT-202-11-A


title: MUC22613 CM-OXT-202-11-B


title: MUC22606 CM-OXT-202-10-K


title: MUC22629 CM-OXT-202-12-B


title: MUC22604 CM-OXT-202-10-I


title: MUC22605 CM-OXT-202-10-J


title: MUC22652 CM-OXT-202-13-I


title: MUC22600 CM-OXT-202-10-E


title: MUC22599 CM-OXT-202-10-D


title: MUC22601 CM-OXT-202-10-F


title: MUC22596 CM-OXT-202-10-A


title: MUC22602 CM-OXT-202-10-G


title: MUC22598 CM-OXT-202-10-C


title: MUC22597 CM-OXT-202-10-B


title: MUC22592 CM-OXT-202-9-M


title: MUC22593 CM-OXT-202-9-N


title: MUC22591 CM-OXT-202-9-L


title: MUC22590 CM-OXT-202-9-K


title: MUC22585 CM-OXT-202-9-F


title: MUC22608 CM-OXT-202-10-M


title: MUC22584 CM-OXT-202-9-E


title: MUC22586 CM-OXT-202-9-G


title: MUC22582 CM-OXT-202-9-C


title: MUC22587 CM-OXT-202-9-H


title: MUC22579 CM-OXT-202-8-P


title: MUC22577 CM-OXT-202-8-N


title: MUC22603 CM-OXT-202-10-H


title: MUC22576 CM-OXT-202-8-M


title: MUC22594 CM-OXT-202-9-O


title: MUC22578 CM-OXT-202-8-O


title: MUC22583 CM-OXT-202-9-D


title: MUC22595 CM-OXT-202-9-P


title: MUC22573 CM-OXT-202-8-J


title: MUC22572 CM-OXT-202-8-I


title: MUC22570 CM-OXT-202-8-G


title: MUC22566 CM-OXT-202-8-C


title: MUC22567 CM-OXT-202-8-D


title: MUC22574 CM-OXT-202-8-K


title: MUC22568 CM-OXT-202-8-E


title: MUC22581 CM-OXT-202-9-B


title: MUC22565 CM-OXT-202-8-B


title: MUC22569 CM-OXT-202-8-F


title: MUC22580 CM-OXT-202-9-A


title: MUC22563 CM-OXT-202-7-P


title: MUC22561 CM-OXT-202-7-N


title: MUC22559 CM-OXT-202-7-L


title: MUC22588 CM-OXT-202-9-I


title: MUC22560 CM-OXT-202-7-M


title: MUC22575 CM-OXT-202-8-L


title: MUC22562 CM-OXT-202-7-O


title: MUC22556 CM-OXT-202-7-I


title: MUC22558 CM-OXT-202-7-K


title: MUC22555 CM-OXT-202-7-H


title: MUC22557 CM-OXT-202-7-J


title: MUC22554 CM-OXT-202-7-G


title: MUC22552 CM-OXT-202-7-E


title: MUC22553 CM-OXT-202-7-F


title: MUC22551 CM-OXT-202-7-D


title: MUC22571 CM-OXT-202-8-H


title: MUC22550 CM-OXT-202-7-C


title: MUC22549 CM-OXT-202-7-B


title: MUC22548 CM-OXT-202-7-A


title: MUC22546 CM-OXT-202-6-O


title: MUC22543 CM-OXT-202-6-L


title: MUC22544 CM-OXT-202-6-M


title: MUC22542 CM-OXT-202-6-K


title: MUC22547 CM-OXT-202-6-P


title: MUC22541 CM-OXT-202-6-J


title: MUC22545 CM-OXT-202-6-N


title: MUC22564 CM-OXT-202-8-A


title: MUC22540 CM-OXT-202-6-I


title: MUC22539 CM-OXT-202-6-H


title: MUC22538 CM-OXT-202-6-G


title: MUC22536 CM-OXT-202-6-E


title: MUC22537 CM-OXT-202-6-F


title: MUC22533 CM-OXT-202-6-B


title: MUC22532 CM-OXT-202-6-A


title: MUC22531 CM-OXT-202-5-P


title: MUC22526 CM-OXT-202-5-K


title: MUC22525 CM-OXT-202-5-J


title: MUC22524 CM-OXT-202-5-I


title: MUC22523 CM-OXT-202-5-H


title: MUC22522 CM-OXT-202-5-G


title: MUC22511 CM-OXT-202-4-L


title: MUC22515 CM-OXT-202-4-P


title: MUC22517 CM-OXT-202-5-B


title: MUC22516 CM-OXT-202-5-A


title: MUC22534 CM-OXT-202-6-C


title: MUC22519 CM-OXT-202-5-D


title: MUC22514 CM-OXT-202-4-O


title: MUC22513 CM-OXT-202-4-N


title: MUC22521 CM-OXT-202-5-F


title: MUC22520 CM-OXT-202-5-E


title: MUC22512 CM-OXT-202-4-M


title: MUC22518 CM-OXT-202-5-C


title: MUC22509 CM-OXT-202-4-J


title: MUC22510 CM-OXT-202-4-K


title: MUC22528 CM-OXT-202-5-M


title: MUC22529 CM-OXT-202-5-N


title: MUC22507 CM-OXT-202-4-H


title: MUC22527 CM-OXT-202-5-L


title: MUC22506 CM-OXT-202-4-G


title: MUC22505 CM-OXT-202-4-F


title: MUC22535 CM-OXT-202-6-D


title: MUC22530 CM-OXT-202-5-O


title: MUC22501 CM-OXT-202-4-B


title: MUC22499 CM-OXT-202-3-P


title: MUC22500 CM-OXT-202-4-A


title: MUC22503 CM-OXT-202-4-D


title: MUC22502 CM-OXT-202-4-C


title: MUC22498 CM-OXT-202-3-O


title: MUC22508 CM-OXT-202-4-I


title: MUC22497 CM-OXT-202-3-N


title: MUC22496 CM-OXT-202-3-M


title: MUC22495 CM-OXT-202-3-L


title: MUC22504 CM-OXT-202-4-E


title: MUC22493 CM-OXT-202-3-J


title: MUC22491 CM-OXT-202-3-H


title: MUC22490 CM-OXT-202-3-G


title: MUC22494 CM-OXT-202-3-K


title: MUC22492 CM-OXT-202-3-I


title: MUC22487 CM-OXT-202-3-D


title: MUC22488 CM-OXT-202-3-E


title: MUC22484 CM-OXT-202-3-A


title: MUC22483 CM-OXT-202-2-P


title: MUC22480 CM-OXT-202-2-M


title: MUC22482 CM-OXT-202-2-O


title: MUC22485 CM-OXT-202-3-B


title: MUC22489 CM-OXT-202-3-F


title: MUC22477 CM-OXT-202-2-J


title: MUC22479 CM-OXT-202-2-L


title: MUC22478 CM-OXT-202-2-K


title: MUC22589 CM-OXT-202-9-J


title: MUC22481 CM-OXT-202-2-N


title: MUC22475 CM-OXT-202-2-H


title: MUC22473 CM-OXT-202-2-F


title: MUC22476 CM-OXT-202-2-I


title: MUC22486 CM-OXT-202-3-C


title: MUC22474 CM-OXT-202-2-G


title: MUC22470 CM-OXT-202-2-C


title: MUC22471 CM-OXT-202-2-D


title: MUC22468 CM-OXT-202-2-A


title: MUC22464 CM-OXT-202-1-M


title: MUC22461 CM-OXT-202-1-J


title: MUC22467 CM-OXT-202-1-P


title: MUC22460 CM-OXT-202-1-I


title: MUC22462 CM-OXT-202-1-K


title: MUC22466 CM-OXT-202-1-O


title: MUC22459 CM-OXT-202-1-H


title: MUC22465 CM-OXT-202-1-N


title: MUC22463 CM-OXT-202-1-L


title: MUC22457 CM-OXT-202-1-F


title: MUC22458 CM-OXT-202-1-G


title: MUC22455 CM-OXT-202-1-D


title: MUC22454 CM-OXT-202-1-C


title: MUC22456 CM-OXT-202-1-E


title: MUC22453 CM-OXT-202-1-B


title: MUC22472 CM-OXT-202-2-E


title: MUC22450 CM-OXT-201-24-O


title: MUC22452 CM-OXT-202-1-A


title: MUC22451 CM-OXT-201-24-P


title: MUC22446 CM-OXT-201-24-K


title: MUC22448 CM-OXT-201-24-M


title: MUC22447 CM-OXT-201-24-L


title: MUC22445 CM-OXT-201-24-J


title: MUC22449 CM-OXT-201-24-N


title: MUC22442 CM-OXT-201-24-G


title: MUC22444 CM-OXT-201-24-I


title: MUC22441 CM-OXT-201-24-F


title: MUC22443 CM-OXT-201-24-H


title: MUC22469 CM-OXT-202-2-B


title: MUC22440 CM-OXT-201-24-E


title: MUC22436 CM-OXT-201-24-A


title: MUC22437 CM-OXT-201-24-B


title: MUC22432 CM-OXT-201-23-M


title: MUC22431 CM-OXT-201-23-L


title: MUC22435 CM-OXT-201-23-P


title: MUC22434 CM-OXT-201-23-O


title: MUC22433 CM-OXT-201-23-N


title: MUC22430 CM-OXT-201-23-K


title: MUC22427 CM-OXT-201-23-H


title: MUC22426 CM-OXT-201-23-G


title: MUC22429 CM-OXT-201-23-J


title: MUC22425 CM-OXT-201-23-F


title: MUC22421 CM-OXT-201-23-B


title: MUC22418 CM-OXT-201-22-O


title: MUC22422 CM-OXT-201-23-C


title: MUC22417 CM-OXT-201-22-N


title: MUC22416 CM-OXT-201-22-M


title: MUC22419 CM-OXT-201-22-P


title: MUC22424 CM-OXT-201-23-E


title: MUC22413 CM-OXT-201-22-J


title: MUC22415 CM-OXT-201-22-L


title: MUC22411 CM-OXT-201-22-H


title: MUC22412 CM-OXT-201-22-I


title: MUC22420 CM-OXT-201-23-A


title: MUC22410 CM-OXT-201-22-G


title: MUC22423 CM-OXT-201-23-D


title: MUC22439 CM-OXT-201-24-D


title: MUC22414 CM-OXT-201-22-K


title: MUC22438 CM-OXT-201-24-C


title: MUC22409 CM-OXT-201-22-F


title: MUC22407 CM-OXT-201-22-D


title: MUC22428 CM-OXT-201-23-I


title: MUC22406 CM-OXT-201-22-C


title: MUC22404 CM-OXT-201-22-A


title: MUC22405 CM-OXT-201-22-B


title: MUC22402 CM-OXT-201-21-O


title: MUC22403 CM-OXT-201-21-P


title: MUC22401 CM-OXT-201-21-N


title: MUC22399 CM-OXT-201-21-L


title: MUC22398 CM-OXT-201-21-K


title: MUC22400 CM-OXT-201-21-M


title: MUC22397 CM-OXT-201-21-J


title: MUC22396 CM-OXT-201-21-I


title: MUC22395 CM-OXT-201-21-H


title: MUC22392 CM-OXT-201-21-E


title: MUC22393 CM-OXT-201-21-F


title: MUC22391 CM-OXT-201-21-D


title: MUC22408 CM-OXT-201-22-E


title: MUC22383 CM-OXT-201-20-L


title: MUC22384 CM-OXT-201-20-M


title: MUC22388 CM-OXT-201-21-A


title: MUC22382 CM-OXT-201-20-K


title: MUC22389 CM-OXT-201-21-B


title: MUC22381 CM-OXT-201-20-J


title: MUC22387 CM-OXT-201-20-P


title: MUC22386 CM-OXT-201-20-O


title: MUC22377 CM-OXT-201-20-F


title: MUC22375 CM-OXT-201-20-D


title: MUC22376 CM-OXT-201-20-E


title: MUC22379 CM-OXT-201-20-H


title: MUC22374 CM-OXT-201-20-C


title: MUC22378 CM-OXT-201-20-G


title: MUC22373 CM-OXT-201-20-B


title: MUC22371 CM-OXT-201-19-P


title: MUC22369 CM-OXT-201-19-N


title: MUC22370 CM-OXT-201-19-O


title: MUC22394 CM-OXT-201-21-G


title: MUC22372 CM-OXT-201-20-A


title: MUC22366 CM-OXT-201-19-K


title: MUC22367 CM-OXT-201-19-L


title: MUC22368 CM-OXT-201-19-M


title: MUC22390 CM-OXT-201-21-C


title: MUC22365 CM-OXT-201-19-J


title: MUC22364 CM-OXT-201-19-I


title: MUC22385 CM-OXT-201-20-N


title: MUC22363 CM-OXT-201-19-H


title: MUC22362 CM-OXT-201-19-G


title: MUC22380 CM-OXT-201-20-I


title: MUC22361 CM-OXT-201-19-F


title: MUC22360 CM-OXT-201-19-E


title: MUC22359 CM-OXT-201-19-D


title: MUC22358 CM-OXT-201-19-C


title: MUC22357 CM-OXT-201-19-B


title: MUC22355 CM-OXT-201-18-P


title: MUC22354 CM-OXT-201-18-O


title: MUC22356 CM-OXT-201-19-A


title: MUC22351 CM-OXT-201-18-L


title: MUC22352 CM-OXT-201-18-M


title: MUC22347 CM-OXT-201-18-H


title: MUC22348 CM-OXT-201-18-I


title: MUC22346 CM-OXT-201-18-G


title: MUC22349 CM-OXT-201-18-J


title: MUC22342 CM-OXT-201-18-C


title: MUC22340 CM-OXT-201-18-A


title: MUC22341 CM-OXT-201-18-B


title: MUC22345 CM-OXT-201-18-F


title: MUC22338 CM-OXT-201-17-O


title: MUC22343 CM-OXT-201-18-D


title: MUC22335 CM-OXT-201-17-L


title: MUC22333 CM-OXT-201-17-J


title: MUC22334 CM-OXT-201-17-K


title: MUC22336 CM-OXT-201-17-M


title: MUC22339 CM-OXT-201-17-P


title: MUC22332 CM-OXT-201-17-I


title: MUC22337 CM-OXT-201-17-N


title: MUC22331 CM-OXT-201-17-H


title: MUC22330 CM-OXT-201-17-G


title: MUC22329 CM-OXT-201-17-F


title: MUC22350 CM-OXT-201-18-K


title: MUC22326 CM-OXT-201-17-C


title: MUC22325 CM-OXT-201-17-B


title: MUC22327 CM-OXT-201-17-D


title: MUC22353 CM-OXT-201-18-N


title: MUC22328 CM-OXT-201-17-E


title: MUC22324 CM-OXT-201-17-A


title: MUC22322 CM-OXT-201-16-O


title: MUC22323 CM-OXT-201-16-P


title: MUC22320 CM-OXT-201-16-M


title: MUC22321 CM-OXT-201-16-N


title: MUC22344 CM-OXT-201-18-E


title: MUC22318 CM-OXT-201-16-K


title: MUC22319 CM-OXT-201-16-L


title: MUC22317 CM-OXT-201-16-J


title: MUC22313 CM-OXT-201-16-F


title: MUC22315 CM-OXT-201-16-H


title: MUC22312 CM-OXT-201-16-E


title: MUC22314 CM-OXT-201-16-G


title: MUC22316 CM-OXT-201-16-I


title: MUC22310 CM-OXT-201-16-C


title: MUC22311 CM-OXT-201-16-D


title: MUC22308 CM-OXT-201-16-A


title: MUC22309 CM-OXT-201-16-B


title: MUC22307 CM-OXT-201-15-P


title: MUC22305 CM-OXT-201-15-N


title: MUC22304 CM-OXT-201-15-M


title: MUC22303 CM-OXT-201-15-L


title: MUC22306 CM-OXT-201-15-O


title: MUC22302 CM-OXT-201-15-K


title: MUC22301 CM-OXT-201-15-J


title: MUC22298 CM-OXT-201-15-G


title: MUC22296 CM-OXT-201-15-E


title: MUC22300 CM-OXT-201-15-I


title: MUC22295 CM-OXT-201-15-D


title: MUC22293 CM-OXT-201-15-B


title: MUC22294 CM-OXT-201-15-C


title: MUC22290 CM-OXT-201-14-O


title: MUC22292 CM-OXT-201-15-A


title: MUC22291 CM-OXT-201-14-P


title: MUC22288 CM-OXT-201-14-M


title: MUC22287 CM-OXT-201-14-L


title: MUC22289 CM-OXT-201-14-N


title: MUC22285 CM-OXT-201-14-J


title: MUC22286 CM-OXT-201-14-K


title: MUC22283 CM-OXT-201-14-H


title: MUC22284 CM-OXT-201-14-I


title: MUC22281 CM-OXT-201-14-F


title: MUC22299 CM-OXT-201-15-H


title: MUC22279 CM-OXT-201-14-D


title: MUC22278 CM-OXT-201-14-C


title: MUC22280 CM-OXT-201-14-E


title: MUC22297 CM-OXT-201-15-F


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title: MUC22275 CM-OXT-201-13-P


title: MUC22273 CM-OXT-201-13-N


title: MUC22272 CM-OXT-201-13-M


title: MUC22274 CM-OXT-201-13-O


title: MUC22271 CM-OXT-201-13-L


title: MUC22269 CM-OXT-201-13-J


title: MUC22267 CM-OXT-201-13-H


title: MUC22268 CM-OXT-201-13-I


title: MUC22270 CM-OXT-201-13-K


title: MUC22265 CM-OXT-201-13-F


title: MUC22264 CM-OXT-201-13-E


title: MUC22262 CM-OXT-201-13-C


title: MUC22266 CM-OXT-201-13-G


title: MUC22261 CM-OXT-201-13-B


title: MUC22282 CM-OXT-201-14-G


title: MUC22263 CM-OXT-201-13-D


title: MUC22259 CM-OXT-201-12-P


title: MUC22257 CM-OXT-201-12-N


title: MUC22258 CM-OXT-201-12-O


title: MUC22255 CM-OXT-201-12-L


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title: MUC22254 CM-OXT-201-12-K


title: MUC22251 CM-OXT-201-12-H


title: MUC22252 CM-OXT-201-12-I


title: MUC22248 CM-OXT-201-12-E


title: MUC22250 CM-OXT-201-12-G


title: MUC22249 CM-OXT-201-12-F


title: MUC22253 CM-OXT-201-12-J


title: MUC22245 CM-OXT-201-12-B


title: MUC22244 CM-OXT-201-12-A


title: MUC22246 CM-OXT-201-12-C


title: MUC22243 CM-OXT-201-11-P


title: MUC22242 CM-OXT-201-11-O


title: MUC22247 CM-OXT-201-12-D


title: MUC22239 CM-OXT-201-11-L


title: MUC22241 CM-OXT-201-11-N


title: MUC22238 CM-OXT-201-11-K


title: MUC22237 CM-OXT-201-11-J


title: MUC22260 CM-OXT-201-13-A


title: MUC22256 CM-OXT-201-12-M


title: MUC22233 CM-OXT-201-11-F


title: MUC22232 CM-OXT-201-11-E


title: MUC22231 CM-OXT-201-11-D


title: MUC22230 CM-OXT-201-11-C


title: MUC22235 CM-OXT-201-11-H


title: MUC22229 CM-OXT-201-11-B


title: MUC22227 CM-OXT-201-10-P


title: MUC22228 CM-OXT-201-11-A


title: MUC22226 CM-OXT-201-10-O


title: MUC22225 CM-OXT-201-10-N


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title: MUC22222 CM-OXT-201-10-K


title: MUC22221 CM-OXT-201-10-J


title: MUC22219 CM-OXT-201-10-H


title: MUC22223 CM-OXT-201-10-L


title: MUC22220 CM-OXT-201-10-I


title: MUC22217 CM-OXT-201-10-F


title: MUC22218 CM-OXT-201-10-G


title: MUC22240 CM-OXT-201-11-M


title: MUC22215 CM-OXT-201-10-D


title: MUC22216 CM-OXT-201-10-E


title: MUC22214 CM-OXT-201-10-C


title: MUC22213 CM-OXT-201-10-B


title: MUC22212 CM-OXT-201-10-A


title: MUC22209 CM-OXT-201-9-N


title: MUC22236 CM-OXT-201-11-I


title: MUC22210 CM-OXT-201-9-O


title: MUC22206 CM-OXT-201-9-K


title: MUC22208 CM-OXT-201-9-M


title: MUC22203 CM-OXT-201-9-H


title: MUC22207 CM-OXT-201-9-L


title: MUC22204 CM-OXT-201-9-I


title: MUC22201 CM-OXT-201-9-F


title: MUC22224 CM-OXT-201-10-M


title: MUC22200 CM-OXT-201-9-E


title: MUC22199 CM-OXT-201-9-D


title: MUC22198 CM-OXT-201-9-C


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title: MUC22192 CM-OXT-201-8-M


title: MUC22191 CM-OXT-201-8-L


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title: MUC22188 CM-OXT-201-8-I


title: MUC22189 CM-OXT-201-8-J


title: MUC22186 CM-OXT-201-8-G


title: MUC22194 CM-OXT-201-8-O


title: MUC22184 CM-OXT-201-8-E


title: MUC22183 CM-OXT-201-8-D


title: MUC22202 CM-OXT-201-9-G


title: MUC22181 CM-OXT-201-8-B


title: MUC22182 CM-OXT-201-8-C


title: MUC22205 CM-OXT-201-9-J


title: MUC22211 CM-OXT-201-9-P


title: MUC22180 CM-OXT-201-8-A


title: MUC22176 CM-OXT-201-7-M


title: MUC22195 CM-OXT-201-8-P


title: MUC22179 CM-OXT-201-7-P


title: MUC22177 CM-OXT-201-7-N


title: MUC22175 CM-OXT-201-7-L


title: MUC22174 CM-OXT-201-7-K


title: MUC22197 CM-OXT-201-9-B


title: MUC22173 CM-OXT-201-7-J


title: MUC22172 CM-OXT-201-7-I


title: MUC22190 CM-OXT-201-8-K


title: MUC22171 CM-OXT-201-7-H


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title: MUC22170 CM-OXT-201-7-G


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title: MUC22168 CM-OXT-201-7-E


title: MUC22167 CM-OXT-201-7-D


title: MUC22164 CM-OXT-201-7-A


title: MUC22185 CM-OXT-201-8-F


title: MUC22163 CM-OXT-201-6-P


title: MUC22165 CM-OXT-201-7-B


title: MUC22161 CM-OXT-201-6-N


title: MUC22178 CM-OXT-201-7-O


title: MUC22160 CM-OXT-201-6-M


title: MUC22159 CM-OXT-201-6-L


title: MUC22158 CM-OXT-201-6-K


title: MUC22162 CM-OXT-201-6-O


title: MUC22155 CM-OXT-201-6-H


title: MUC22154 CM-OXT-201-6-G


title: MUC22157 CM-OXT-201-6-J


title: MUC22156 CM-OXT-201-6-I


title: MUC22151 CM-OXT-201-6-D


title: MUC22152 CM-OXT-201-6-E


title: MUC22149 CM-OXT-201-6-B


title: MUC22150 CM-OXT-201-6-C


title: MUC22153 CM-OXT-201-6-F


title: MUC22147 CM-OXT-201-5-P


title: MUC22148 CM-OXT-201-6-A


title: MUC22166 CM-OXT-201-7-C


title: MUC22146 CM-OXT-201-5-O


title: MUC22143 CM-OXT-201-5-L


title: MUC22145 CM-OXT-201-5-N


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title: MUC22141 CM-OXT-201-5-J


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title: MUC22138 CM-OXT-201-5-G


title: MUC22139 CM-OXT-201-5-H


title: MUC22137 CM-OXT-201-5-F


title: MUC22135 CM-OXT-201-5-D


title: MUC22133 CM-OXT-201-5-B


title: MUC22132 CM-OXT-201-5-A


title: MUC22131 CM-OXT-201-4-P


title: MUC22130 CM-OXT-201-4-O


title: MUC22129 CM-OXT-201-4-N


title: MUC22128 CM-OXT-201-4-M


title: MUC22134 CM-OXT-201-5-C


title: MUC22126 CM-OXT-201-4-K


title: MUC22125 CM-OXT-201-4-J


title: MUC22144 CM-OXT-201-5-M


title: MUC22127 CM-OXT-201-4-L


title: MUC22123 CM-OXT-201-4-H


title: MUC22122 CM-OXT-201-4-G


title: MUC22117 CM-OXT-201-4-B


title: MUC22118 CM-OXT-201-4-C


title: MUC22120 CM-OXT-201-4-E


title: MUC22121 CM-OXT-201-4-F


title: MUC22124 CM-OXT-201-4-I


title: MUC22119 CM-OXT-201-4-D


title: MUC22116 CM-OXT-201-4-A


title: MUC22114 CM-OXT-201-3-O


title: MUC22115 CM-OXT-201-3-P


title: MUC22136 CM-OXT-201-5-E


title: MUC22113 CM-OXT-201-3-N


title: MUC22112 CM-OXT-201-3-M


title: MUC22108 CM-OXT-201-3-I


title: MUC22109 CM-OXT-201-3-J


title: MUC22107 CM-OXT-201-3-H


title: MUC22111 CM-OXT-201-3-L


title: MUC22106 CM-OXT-201-3-G


title: MUC22104 CM-OXT-201-3-E


title: MUC22105 CM-OXT-201-3-F


title: MUC22103 CM-OXT-201-3-D


title: MUC22100 CM-OXT-201-3-A


title: MUC22099 CM-OXT-201-2-P


title: MUC22096 CM-OXT-201-2-M


title: MUC22095 CM-OXT-201-2-L


title: MUC22097 CM-OXT-201-2-N


title: MUC22098 CM-OXT-201-2-O


title: MUC22093 CM-OXT-201-2-J


title: MUC22090 CM-OXT-201-2-G


title: MUC22092 CM-OXT-201-2-I


title: MUC22089 CM-OXT-201-2-F


title: MUC22094 CM-OXT-201-2-K


title: MUC22088 CM-OXT-201-2-E


title: MUC22091 CM-OXT-201-2-H


title: MUC22110 CM-OXT-201-3-K


title: MUC22086 CM-OXT-201-2-C


title: MUC22084 CM-OXT-201-2-A


title: MUC22085 CM-OXT-201-2-B


title: MUC22083 CM-OXT-201-1-P


title: MUC22082 CM-OXT-201-1-O


title: MUC22081 CM-OXT-201-1-N


title: MUC22079 CM-OXT-201-1-L


title: MUC22080 CM-OXT-201-1-M


title: MUC22101 CM-OXT-201-3-B


title: MUC22102 CM-OXT-201-3-C


title: MUC22078 CM-OXT-201-1-K


title: MUC22077 CM-OXT-201-1-J


title: MUC22076 CM-OXT-201-1-I


title: MUC22075 CM-OXT-201-1-H


title: MUC22072 CM-OXT-201-1-E


title: MUC22070 CM-OXT-201-1-C


title: MUC22074 CM-OXT-201-1-G


title: MUC22069 CM-OXT-201-1-B


title: MUC22073 CM-OXT-201-1-F


title: MUC22068 CM-OXT-201-1-A


title: MUC22063 CM-OXT-102-24-L


title: MUC22065 CM-OXT-102-24-N


title: MUC22067 CM-OXT-102-24-P


title: MUC22066 CM-OXT-102-24-O


title: MUC22087 CM-OXT-201-2-D


title: MUC22060 CM-OXT-102-24-I


title: MUC22064 CM-OXT-102-24-M


title: MUC22061 CM-OXT-102-24-J


title: MUC22059 CM-OXT-102-24-H


title: MUC22058 CM-OXT-102-24-G


title: MUC22057 CM-OXT-102-24-F


title: MUC22055 CM-OXT-102-24-D


title: MUC22054 CM-OXT-102-24-C


title: MUC22053 CM-OXT-102-24-B


title: MUC22052 CM-OXT-102-24-A


title: MUC22051 CM-OXT-102-23-P


title: MUC22050 CM-OXT-102-23-O


title: MUC22047 CM-OXT-102-23-L


title: MUC22048 CM-OXT-102-23-M


title: MUC22071 CM-OXT-201-1-D


title: MUC22046 CM-OXT-102-23-K


title: MUC22045 CM-OXT-102-23-J


title: MUC22044 CM-OXT-102-23-I


title: MUC22043 CM-OXT-102-23-H


title: MUC22062 CM-OXT-102-24-K


title: MUC22042 CM-OXT-102-23-G


title: MUC22041 CM-OXT-102-23-F


title: MUC22039 CM-OXT-102-23-D


title: MUC22036 CM-OXT-102-23-A


title: MUC22035 CM-OXT-102-22-P


title: MUC22034 CM-OXT-102-22-O


title: MUC22032 CM-OXT-102-22-M


title: MUC22038 CM-OXT-102-23-C


title: MUC22037 CM-OXT-102-23-B


title: MUC22056 CM-OXT-102-24-E


title: MUC22040 CM-OXT-102-23-E


title: MUC22030 CM-OXT-102-22-K


title: MUC22031 CM-OXT-102-22-L


title: MUC22033 CM-OXT-102-22-N


title: MUC22049 CM-OXT-102-23-N


title: MUC22029 CM-OXT-102-22-J


title: MUC22028 CM-OXT-102-22-I


title: MUC22026 CM-OXT-102-22-G


title: MUC22024 CM-OXT-102-22-E


title: MUC22023 CM-OXT-102-22-D


title: MUC22021 CM-OXT-102-22-B


title: MUC22027 CM-OXT-102-22-H


title: MUC22019 CM-OXT-102-21-P


title: MUC22020 CM-OXT-102-22-A


title: MUC22016 CM-OXT-102-21-M


title: MUC22017 CM-OXT-102-21-N


title: MUC22018 CM-OXT-102-21-O


title: MUC22015 CM-OXT-102-21-L


title: MUC22014 CM-OXT-102-21-K


title: MUC22013 CM-OXT-102-21-J


title: MUC22012 CM-OXT-102-21-I


title: MUC22011 CM-OXT-102-21-H


title: MUC22009 CM-OXT-102-21-F


title: MUC22008 CM-OXT-102-21-E


title: MUC22007 CM-OXT-102-21-D


title: MUC22010 CM-OXT-102-21-G


title: MUC22006 CM-OXT-102-21-C


title: MUC22005 CM-OXT-102-21-B


title: MUC22004 CM-OXT-102-21-A


title: MUC22003 CM-OXT-102-20-P


title: MUC22000 CM-OXT-102-20-M


title: MUC22002 CM-OXT-102-20-O


title: MUC22025 CM-OXT-102-22-F


title: MUC21999 CM-OXT-102-20-L


title: MUC21997 CM-OXT-102-20-J


title: MUC22001 CM-OXT-102-20-N


title: MUC21995 CM-OXT-102-20-H


title: MUC21998 CM-OXT-102-20-K


title: MUC21996 CM-OXT-102-20-I


title: MUC21993 CM-OXT-102-20-F


title: MUC22022 CM-OXT-102-22-C


title: MUC21994 CM-OXT-102-20-G


title: MUC21992 CM-OXT-102-20-E


title: MUC21990 CM-OXT-102-20-C


title: MUC21988 CM-OXT-102-20-A


title: MUC21991 CM-OXT-102-20-D


title: MUC21989 CM-OXT-102-20-B


title: MUC21986 CM-OXT-102-19-O


title: MUC21987 CM-OXT-102-19-P


title: MUC21985 CM-OXT-102-19-N


title: MUC21984 CM-OXT-102-19-M


title: MUC21981 CM-OXT-102-19-J


title: MUC21983 CM-OXT-102-19-L


title: MUC21982 CM-OXT-102-19-K


title: MUC21980 CM-OXT-102-19-I


title: MUC21979 CM-OXT-102-19-H


title: MUC21978 CM-OXT-102-19-G


title: MUC21977 CM-OXT-102-19-F


title: MUC21976 CM-OXT-102-19-E


title: MUC21975 CM-OXT-102-19-D


title: MUC21974 CM-OXT-102-19-C


title: MUC21973 CM-OXT-102-19-B


title: MUC21972 CM-OXT-102-19-A


title: MUC21970 CM-OXT-102-18-O


title: MUC21969 CM-OXT-102-18-N


title: MUC21971 CM-OXT-102-18-P


title: MUC21966 CM-OXT-102-18-K


title: MUC21968 CM-OXT-102-18-M


title: MUC21965 CM-OXT-102-18-J


title: MUC21964 CM-OXT-102-18-I


title: MUC21963 CM-OXT-102-18-H


title: MUC21962 CM-OXT-102-18-G


title: MUC21961 CM-OXT-102-18-F


title: MUC21959 CM-OXT-102-18-D


title: MUC21958 CM-OXT-102-18-C


title: MUC21956 CM-OXT-102-18-A


title: MUC21955 CM-OXT-102-17-P


title: MUC21951 CM-OXT-102-17-L


title: MUC21960 CM-OXT-102-18-E


title: MUC21957 CM-OXT-102-18-B


title: MUC21954 CM-OXT-102-17-O


title: MUC21948 CM-OXT-102-17-I


title: MUC21950 CM-OXT-102-17-K


title: MUC21949 CM-OXT-102-17-J


title: MUC21967 CM-OXT-102-18-L


title: MUC21947 CM-OXT-102-17-H


title: MUC21946 CM-OXT-102-17-G


title: MUC21945 CM-OXT-102-17-F


title: MUC21944 CM-OXT-102-17-E


title: MUC21942 CM-OXT-102-17-C


title: MUC21939 CM-OXT-102-16-P


title: MUC21943 CM-OXT-102-17-D


title: MUC21941 CM-OXT-102-17-B


title: MUC21937 CM-OXT-102-16-N


title: MUC21938 CM-OXT-102-16-O


title: MUC21936 CM-OXT-102-16-M


title: MUC21935 CM-OXT-102-16-L


title: MUC21933 CM-OXT-102-16-J


title: MUC21934 CM-OXT-102-16-K


title: MUC21932 CM-OXT-102-16-I


title: MUC21931 CM-OXT-102-16-H


title: MUC21952 CM-OXT-102-17-M


title: MUC21929 CM-OXT-102-16-F


title: MUC21953 CM-OXT-102-17-N


title: MUC21927 CM-OXT-102-16-D


title: MUC21926 CM-OXT-102-16-C


title: MUC21924 CM-OXT-102-16-A


title: MUC21925 CM-OXT-102-16-B


title: MUC21923 CM-OXT-102-15-P


title: MUC21922 CM-OXT-102-15-O


title: MUC21919 CM-OXT-102-15-L


title: MUC21940 CM-OXT-102-17-A


title: MUC21920 CM-OXT-102-15-M


title: MUC21918 CM-OXT-102-15-K


title: MUC21917 CM-OXT-102-15-J


title: MUC21916 CM-OXT-102-15-I


title: MUC21921 CM-OXT-102-15-N


title: MUC21915 CM-OXT-102-15-H


title: MUC21914 CM-OXT-102-15-G


title: MUC21913 CM-OXT-102-15-F


title: MUC21909 CM-OXT-102-15-B


title: MUC21911 CM-OXT-102-15-D


title: MUC21910 CM-OXT-102-15-C


title: MUC21930 CM-OXT-102-16-G


title: MUC21928 CM-OXT-102-16-E


title: MUC21908 CM-OXT-102-15-A


title: MUC21906 CM-OXT-102-14-O


title: MUC21907 CM-OXT-102-14-P


title: MUC21903 CM-OXT-102-14-L


title: MUC21904 CM-OXT-102-14-M


title: MUC21901 CM-OXT-102-14-J


title: MUC21902 CM-OXT-102-14-K


title: MUC21900 CM-OXT-102-14-I


title: MUC21897 CM-OXT-102-14-F


title: MUC21899 CM-OXT-102-14-H


title: MUC21896 CM-OXT-102-14-E


title: MUC21894 CM-OXT-102-14-C


title: MUC21912 CM-OXT-102-15-E


title: MUC21895 CM-OXT-102-14-D


title: MUC21891 CM-OXT-102-13-P


title: MUC21893 CM-OXT-102-14-B


title: MUC21892 CM-OXT-102-14-A


title: MUC21890 CM-OXT-102-13-O


title: MUC21888 CM-OXT-102-13-M


title: MUC21889 CM-OXT-102-13-N


title: MUC21880 CM-OXT-102-13-E


title: MUC21887 CM-OXT-102-13-L


title: MUC21884 CM-OXT-102-13-I


title: MUC21879 CM-OXT-102-13-D


title: MUC21886 CM-OXT-102-13-K


title: MUC21885 CM-OXT-102-13-J


title: MUC21905 CM-OXT-102-14-N


title: MUC21881 CM-OXT-102-13-F


title: MUC21883 CM-OXT-102-13-H


title: MUC21878 CM-OXT-102-13-C


title: MUC21875 CM-OXT-102-12-P


title: MUC21876 CM-OXT-102-13-A


title: MUC21874 CM-OXT-102-12-O


title: MUC21898 CM-OXT-102-14-G


title: MUC21882 CM-OXT-102-13-G


title: MUC21872 CM-OXT-102-12-M


title: MUC21871 CM-OXT-102-12-L


title: MUC21873 CM-OXT-102-12-N


title: MUC21869 CM-OXT-102-12-J


title: MUC21877 CM-OXT-102-13-B


title: MUC21867 CM-OXT-102-12-H


title: MUC21868 CM-OXT-102-12-I


title: MUC21864 CM-OXT-102-12-E


title: MUC21862 CM-OXT-102-12-C


title: MUC21863 CM-OXT-102-12-D


title: MUC21870 CM-OXT-102-12-K


title: MUC21861 CM-OXT-102-12-B


title: MUC21856 CM-OXT-102-11-M


title: MUC21853 CM-OXT-102-11-J


title: MUC21848 CM-OXT-102-11-E


title: MUC21858 CM-OXT-102-11-O


title: MUC21852 CM-OXT-102-11-I


title: MUC21847 CM-OXT-102-11-D


title: MUC21844 CM-OXT-102-11-A


title: MUC21845 CM-OXT-102-11-B


title: MUC21859 CM-OXT-102-11-P


title: MUC21849 CM-OXT-102-11-F


title: MUC21860 CM-OXT-102-12-A


title: MUC21846 CM-OXT-102-11-C


title: MUC21842 CM-OXT-102-10-O


title: MUC21843 CM-OXT-102-10-P


title: MUC21851 CM-OXT-102-11-H


title: MUC21840 CM-OXT-102-10-M


title: MUC21865 CM-OXT-102-12-F


title: MUC21866 CM-OXT-102-12-G


title: MUC21838 CM-OXT-102-10-K


title: MUC21839 CM-OXT-102-10-L


title: MUC21837 CM-OXT-102-10-J


title: MUC21857 CM-OXT-102-11-N


title: MUC21854 CM-OXT-102-11-K


title: MUC21855 CM-OXT-102-11-L


title: MUC21850 CM-OXT-102-11-G


title: MUC21834 CM-OXT-102-10-G


title: MUC21835 CM-OXT-102-10-H


title: MUC21832 CM-OXT-102-10-E


title: MUC21836 CM-OXT-102-10-I


title: MUC21831 CM-OXT-102-10-D


title: MUC21829 CM-OXT-102-10-B


title: MUC21830 CM-OXT-102-10-C


title: MUC21841 CM-OXT-102-10-N


title: MUC21828 CM-OXT-102-10-A


title: MUC21826 CM-OXT-102-9-O


title: MUC21825 CM-OXT-102-9-N


title: MUC21827 CM-OXT-102-9-P


title: MUC21821 CM-OXT-102-9-J


title: MUC21823 CM-OXT-102-9-L


title: MUC21824 CM-OXT-102-9-M


title: MUC21820 CM-OXT-102-9-I


title: MUC21833 CM-OXT-102-10-F


title: MUC21822 CM-OXT-102-9-K


title: MUC21818 CM-OXT-102-9-G


title: MUC21817 CM-OXT-102-9-F


title: MUC21815 CM-OXT-102-9-D


title: MUC21816 CM-OXT-102-9-E


title: MUC21811 CM-OXT-102-8-P


title: MUC21813 CM-OXT-102-9-B


title: MUC21812 CM-OXT-102-9-A


title: MUC21808 CM-OXT-102-8-M


title: MUC21814 CM-OXT-102-9-C


title: MUC21809 CM-OXT-102-8-N


title: MUC21810 CM-OXT-102-8-O


title: MUC21807 CM-OXT-102-8-L


title: MUC21799 CM-OXT-102-8-D


title: MUC21806 CM-OXT-102-8-K


title: MUC21801 CM-OXT-102-8-F


title: MUC21803 CM-OXT-102-8-H


title: MUC21796 CM-OXT-102-8-A


title: MUC21805 CM-OXT-102-8-J


title: MUC21797 CM-OXT-102-8-B


title: MUC21819 CM-OXT-102-9-H


title: MUC21802 CM-OXT-102-8-G


title: MUC21800 CM-OXT-102-8-E


title: MUC21792 CM-OXT-102-7-M


title: MUC21794 CM-OXT-102-7-O


title: MUC21795 CM-OXT-102-7-P


title: MUC21793 CM-OXT-102-7-N


title: MUC21789 CM-OXT-102-7-J


title: MUC21790 CM-OXT-102-7-K


title: MUC21791 CM-OXT-102-7-L


title: MUC21787 CM-OXT-102-7-H


title: MUC21788 CM-OXT-102-7-I


title: MUC21786 CM-OXT-102-7-G


title: MUC21798 CM-OXT-102-8-C


title: MUC21804 CM-OXT-102-8-I


title: MUC21781 CM-OXT-102-7-B


title: MUC21784 CM-OXT-102-7-E


title: MUC21785 CM-OXT-102-7-F


title: MUC21780 CM-OXT-102-7-A


title: MUC21782 CM-OXT-102-7-C


title: MUC21783 CM-OXT-102-7-D


title: MUC21775 CM-OXT-102-6-L


title: MUC21779 CM-OXT-102-6-P


title: MUC21776 CM-OXT-102-6-M


title: MUC21778 CM-OXT-102-6-O


title: MUC21768 CM-OXT-102-6-E


title: MUC21773 CM-OXT-102-6-J


title: MUC21772 CM-OXT-102-6-I


title: MUC21777 CM-OXT-102-6-N


title: MUC21766 CM-OXT-102-6-C


title: MUC21774 CM-OXT-102-6-K


title: MUC21769 CM-OXT-102-6-F


title: MUC21765 CM-OXT-102-6-B


title: MUC21770 CM-OXT-102-6-G


title: MUC21761 CM-OXT-102-5-N


title: MUC21760 CM-OXT-102-5-M


title: MUC21759 CM-OXT-102-5-L


title: MUC21764 CM-OXT-102-6-A


title: MUC21762 CM-OXT-102-5-O


title: MUC21767 CM-OXT-102-6-D


title: MUC21758 CM-OXT-102-5-K


title: MUC21771 CM-OXT-102-6-H


title: MUC21757 CM-OXT-102-5-J


title: MUC21756 CM-OXT-102-5-I


title: MUC21755 CM-OXT-102-5-H


title: MUC21751 CM-OXT-102-5-D


title: MUC21753 CM-OXT-102-5-F


title: MUC21750 CM-OXT-102-5-C


title: MUC21754 CM-OXT-102-5-G


title: MUC21752 CM-OXT-102-5-E


title: MUC21749 CM-OXT-102-5-B


title: MUC21748 CM-OXT-102-5-A


title: MUC21747 CM-OXT-102-4-P


title: MUC21745 CM-OXT-102-4-N


title: MUC21746 CM-OXT-102-4-O


title: MUC21744 CM-OXT-102-4-M


title: MUC21763 CM-OXT-102-5-P


title: MUC21743 CM-OXT-102-4-L


title: MUC21742 CM-OXT-102-4-K


title: MUC21735 CM-OXT-102-4-D


title: MUC21733 CM-OXT-102-4-B


title: MUC21740 CM-OXT-102-4-I


title: MUC21732 CM-OXT-102-4-A


title: MUC21738 CM-OXT-102-4-G


title: MUC21739 CM-OXT-102-4-H


title: MUC21730 CM-OXT-102-3-O


title: MUC21737 CM-OXT-102-4-F


title: MUC21734 CM-OXT-102-4-C


title: MUC21728 CM-OXT-102-3-M


title: MUC21727 CM-OXT-102-3-L


title: MUC21729 CM-OXT-102-3-N


title: MUC21725 CM-OXT-102-3-J


title: MUC21724 CM-OXT-102-3-I


title: MUC21723 CM-OXT-102-3-H


title: MUC21721 CM-OXT-102-3-F


title: MUC21720 CM-OXT-102-3-E


title: MUC21722 CM-OXT-102-3-G


title: MUC21741 CM-OXT-102-4-J


title: MUC21719 CM-OXT-102-3-D


title: MUC21718 CM-OXT-102-3-C


title: MUC21716 CM-OXT-102-3-A


title: MUC21715 CM-OXT-102-2-P


title: MUC21736 CM-OXT-102-4-E


title: MUC21717 CM-OXT-102-3-B


title: MUC21714 CM-OXT-102-2-O


title: MUC21713 CM-OXT-102-2-N


title: MUC21711 CM-OXT-102-2-L


title: MUC21712 CM-OXT-102-2-M


title: MUC21710 CM-OXT-102-2-K


title: MUC21709 CM-OXT-102-2-J


title: MUC21731 CM-OXT-102-3-P


title: MUC21706 CM-OXT-102-2-G


title: MUC21705 CM-OXT-102-2-F


title: MUC21707 CM-OXT-102-2-H


title: MUC21726 CM-OXT-102-3-K


title: MUC21703 CM-OXT-102-2-D


title: MUC21704 CM-OXT-102-2-E


title: MUC21702 CM-OXT-102-2-C


title: MUC21700 CM-OXT-102-2-A


title: MUC21698 CM-OXT-102-1-O


title: MUC21692 CM-OXT-102-1-I


title: MUC21697 CM-OXT-102-1-N


title: MUC21696 CM-OXT-102-1-M


title: MUC21699 CM-OXT-102-1-P


title: MUC21694 CM-OXT-102-1-K


title: MUC21695 CM-OXT-102-1-L


title: MUC21701 CM-OXT-102-2-B


title: MUC21691 CM-OXT-102-1-H


title: MUC21693 CM-OXT-102-1-J


title: MUC21690 CM-OXT-102-1-G


title: MUC21689 CM-OXT-102-1-F


title: MUC21688 CM-OXT-102-1-E


title: MUC21686 CM-OXT-102-1-C


title: MUC21687 CM-OXT-102-1-D


title: MUC21684 CM-OXT-102-1-A


title: MUC21682 CM-OXT-101-24-O


title: MUC21681 CM-OXT-101-24-N


title: MUC21708 CM-OXT-102-2-I


title: MUC21683 CM-OXT-101-24-P


title: MUC21680 CM-OXT-101-24-M


title: MUC21677 CM-OXT-101-24-J


title: MUC21678 CM-OXT-101-24-K


title: MUC21674 CM-OXT-101-24-G


title: MUC21672 CM-OXT-101-24-E


title: MUC21673 CM-OXT-101-24-F


title: MUC21676 CM-OXT-101-24-I


title: MUC21671 CM-OXT-101-24-D


title: MUC21668 CM-OXT-101-24-A


title: MUC21669 CM-OXT-101-24-B


title: MUC21667 CM-OXT-101-23-P


title: MUC21685 CM-OXT-102-1-B


title: MUC21665 CM-OXT-101-23-N


title: MUC21662 CM-OXT-101-23-K


title: MUC21661 CM-OXT-101-23-J


title: MUC21675 CM-OXT-101-24-H


title: MUC21670 CM-OXT-101-24-C


title: MUC21658 CM-OXT-101-23-G


title: MUC21666 CM-OXT-101-23-O


title: MUC21664 CM-OXT-101-23-M


title: MUC21663 CM-OXT-101-23-L


title: MUC21659 CM-OXT-101-23-H


title: MUC21655 CM-OXT-101-23-D


title: MUC21654 CM-OXT-101-23-C


title: MUC21660 CM-OXT-101-23-I


title: MUC21657 CM-OXT-101-23-F


title: MUC21679 CM-OXT-101-24-L


title: MUC21652 CM-OXT-101-23-A


title: MUC21656 CM-OXT-101-23-E


title: MUC21653 CM-OXT-101-23-B


title: MUC21651 CM-OXT-101-22-P


title: MUC21650 CM-OXT-101-22-O


title: MUC21649 CM-OXT-101-22-N


title: MUC21646 CM-OXT-101-22-K


title: MUC21647 CM-OXT-101-22-L


title: MUC21648 CM-OXT-101-22-M


title: MUC21645 CM-OXT-101-22-J


title: MUC21644 CM-OXT-101-22-I


title: MUC21643 CM-OXT-101-22-H


title: MUC21642 CM-OXT-101-22-G


title: MUC21641 CM-OXT-101-22-F


title: MUC21638 CM-OXT-101-22-C


title: MUC21636 CM-OXT-101-22-A


title: MUC21640 CM-OXT-101-22-E


title: MUC21635 CM-OXT-101-21-P


title: MUC21633 CM-OXT-101-21-N


title: MUC21637 CM-OXT-101-22-B


title: MUC21630 CM-OXT-101-21-K


title: MUC21634 CM-OXT-101-21-O


title: MUC21632 CM-OXT-101-21-M


title: MUC21626 CM-OXT-101-21-G


title: MUC21628 CM-OXT-101-21-I


title: MUC21625 CM-OXT-101-21-F


title: MUC21624 CM-OXT-101-21-E


title: MUC21629 CM-OXT-101-21-J


title: MUC21621 CM-OXT-101-21-B


title: MUC21622 CM-OXT-101-21-C


title: MUC21623 CM-OXT-101-21-D


title: MUC21618 CM-OXT-101-20-O


title: MUC21619 CM-OXT-101-20-P


title: MUC21639 CM-OXT-101-22-D


title: MUC21620 CM-OXT-101-21-A


title: MUC21616 CM-OXT-101-20-M


title: MUC21617 CM-OXT-101-20-N


title: MUC21631 CM-OXT-101-21-L


title: MUC21615 CM-OXT-101-20-L


title: MUC21627 CM-OXT-101-21-H


title: MUC21613 CM-OXT-101-20-J


title: MUC21614 CM-OXT-101-20-K


title: MUC21610 CM-OXT-101-20-G


title: MUC21609 CM-OXT-101-20-F


title: MUC21607 CM-OXT-101-20-D


title: MUC21608 CM-OXT-101-20-E


title: MUC21611 CM-OXT-101-20-H


title: MUC21606 CM-OXT-101-20-C


title: 293T- U1 siRNA WT 2

city: Tel Aviv

first: Guy

last: Shapira

address: Chaim Levanon 30

country: Israel

institute: Tel Aviv University

data_processing:
mixture-of-isoforms (MISO) analysis
Alignment and gene expression quantification via STAR aligner version 2.7.2a
Assembly: Human genome reference sequence GRCh37 (hg19) and transcriptome (GTF transcript annotation retrieved from UCSC)
Supplementary files format and content: Gene quantification results in txt format (raw counts)

extract_protocol:
Total RNA was extracted from cell cultures using Trizol reagent (Thermo Fisher Scientific, USA). The final RNA concentration and purity were measured using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Thermo Scientific, USA). RNA was purified from the cells 24 hours after transfection.
The RNA libraries were sequenced using Illumina HiSeq2500 sequencing, 20 million reads designated, paired-end sequencing with a 126nt length.

treatment_protocol: HEK 293T cells were seeded in 24-well plates at a concentration of 8×104cells/well. Cells were transfected with 500ng plasmid, using Lipofectamine 2000 Transfection Reagent (Thermo Fisher Scientific, USA) according to the manufacturer’s instructions. Transfection efficiencies were measured by GFP fluorescence in all the cells, indicating a minimum transfection efficiency of 50% repeatedly. U1 was down-regulated using the TriFecta Dicer-Substrate RNAi according to the manufacturer’s protocol (Integrated DNA Technologies, USA). U1 expression was inhibited by 30% using 1nM of the specific siRNA (sequence:  CCACAAATTATGCAGTCGAGTTTCCCA) OE for 24h in the HEK-293T cells.

value: U1 siRNA WT


title: 293T- Scram WT 1

value: Scram WT


title: 293T- Scram WT 3


title: 293T- Scram WT 2


title: MUC21605 CM-OXT-101-20-B


title: 293T- U1 siRNA WT 3


title: MUC21603 CM-OXT-101-19-P


title: 293T- miR-211 3


title: 293T- U1 siRNA WT 1


title: 293T- Control 2

value: Control


title: MUC21612 CM-OXT-101-20-I


title: 293T- Control 1


title: 293T- miR-211 1


title: 293T- miR-211 2


title: MUC21601 CM-OXT-101-19-N


title: NS1983miR

city: Madrid

first: Carmen

middle: Gómez-Escolar

last: Arias

state: Madrid

address: Calle Melchor Fernández Almagro 3

institute: CNIC

description: PDX tumor, miR-28 treated

data_processing:
Basecalls and demultiplexing were performed using CASAVA v1.8
Sequencing adaptors were removed by cutadapt version 1.9
Reads were aligned and quantified to the mouse transcriptome using RSEM
Data were processed by a differential expression analysis pipeline that used Bioconductor package EdgeR for normalization and differential expression testing
Assembly: GRCh38.91
Supplementary files format and content: tab-delimited table of counts

source_name: Subcutaneous PDX tumor

growth_protocol: tumors were grown subcutaneously in NSG mice

extract_protocol:
RNA was harvested using Rneasy mini  kit (Qiagen).
Total RNA was used to generate barcoded RNA-seq libraries using the NEBNext Ultra RNA Library preparation kit (New England Biolabs). First, poly A+ RNA was purified using poly-T oligo- attached magnetic beads followed by fragmentation and first and second cDNA strand synthesis. Next, cDNA ends were repaired and adenylated. The NEBNext adaptor was then ligated followed by Uracile excision from the adaptor and PCR amplification
Libraries were sequenced on a HiSeq2500 (Illumina) to generate 60 bases single reads.

treatment_protocol: control or hsa-miR-28-5p mimics treatment (0.5 nmol) intratumorally in subcutaneous tumors

value: miR-28 mimic treatment

value: DLBCL tumor tissue


title: NS1972miR

description: PDX tumor, miR-28 treated


title: NS1983SCR

description: PDX tumor, control

value: control mimic treatment


title: MUC21600 CM-OXT-101-19-M


title: NS1972SCR

description: PDX tumor, control


title: MUC21599 CM-OXT-101-19-L


title: 293T- Control 3


title: MUC21604 CM-OXT-101-20-A


title: MUC21602 CM-OXT-101-19-O


title: MUC21598 CM-OXT-101-19-K


title: MUC21597 CM-OXT-101-19-J


description: JHH-6 cells transiently transfected with 100 pmol siRNA APE1, replicate 3

data_processing:
Basecalls performed using CASAVA (Illumina Inc.)
Transcript quantification was conducted with STAR (v2.5.3a)  (assigned to a gene using using the STAR function “quantMode GeneCounts”) mapped to the human genome version GRCh38 and with reference annotation.
Read counts generated by STAR were analysed by using DESeq2 package (Love, 2014) for detecting genes that were differentially expressed. An adjusted p-value cut off of 0.05 was decided as threshold for detection of DEGs.
Assembly: human genome version GRCh38 and with reference annotation.
Supplementary files format and content: tab-delimited text files include COUNTS values for each Sample

source_name: liver

extract_protocol:
RNA extraction was carried out using miRNeasy kit (Qiagen, USA) following the manufacturer’s protocols.
Library preparation was performed using SMARTer smRNA-Seq kit (Clontech/Takara, USA) protocol with minor changes to increase the chance of identifying also pri-miR in addition to miRNAs.
RNA-Seq on a Illumina HiSeq 2500  instrument, in rapid run mode, using a 100-bp, dual-indexed, single-end sequencing configuration

value: liver

value: hepatocellular carcinoma cell line


title: JHH-6 cells transiently transfected with 100 pmol siRNA APE1, replicate 4


title: NS1971miR

description: PDX tumor, miR-28 treated


description: JHH-6 cells transiently transfected with 100 pmol scramble control siRNA, replicate 3

value: control


description: JHH-6 cells transiently transfected with 100 pmol scramble control siRNA, replicate 1


description: JHH-6 cells transiently transfected with 100 pmol scramble control siRNA, replicate 2


title: NS1971SCR

description: PDX tumor, control


description: JHH-6 cells transiently transfected with 100 pmol siRNA APE1, replicate 2


title: FOXP3 KO MT-2 biol rep 4

city: Chapel Hill

first: Jason

middle: Robert

last: Mock

state: NC

address: 125 Mason Farm Road

institute: University of North Carolina

data_processing:
Illumina bcl2fastq v2.19 used for basecalling.
Sequence reads in FASTQ files were mapped to GRCh38 reference genome with GENCODE 37 gene annotation by STAR, and gene expression estimates were derived from StringTie using default parameters.
Assembly: GRCh38
Assembly: GENCODE human v37
Supplementary files format and content: tab-delimited text files include FPKM values for each Sample.
Supplementary files format and content: tab-delimited text files include TPM values for each Sample.

source_name: cell line

growth_protocol: MT-2 cell line was obtained through the NIH AIDS Reagent Program. MT-2 cells were maintained in culture using RPMI 1640 supplemented with 10% fetal bovine serum, 2mM L-glutamine, and 100U/mL penicillin-streptomycin.

extract_protocol:
Total RNA was isolated using Zymo Direct-zol RNA MiniPrep with TRI Reagent miRNeasy kits from Zymo Research (Irvine, CA, USA) according to the manufacturer's instruction with the following modifications: 1) snap-frozen cells were lysed in at least 200 mL of TRI reagent without thawing cells; 2) cells were not centrifuged to collect debris before applying to column. ALl samples were eluted in 25 mL of DNase/RNase-Free water.
RNA libraries were prepared for sequencing using standard Illumina protocols.

treatment_protocol: The wild type MT-2 cell line and the FOXP3 null MT-2 cell line were seeded at equal densities and grown in vitro at 37 C 5% CO2 for 3 days before cells were centrifuged at 400 x g for 5 minutes. Supernatant was removed and cell pellets were snap frozen in liquind nitrogen before being stored in liquid nitrogen.  Cell pellets were then prepared for mRNA after 4 separate experiments.

value: cell line

value: regulatory T cell

value: FOXP3 knockout

value: 3 days of in vitro culture


title: JHH-6 cells transiently transfected with 100 pmol scramble control siRNA, replicate 4


title: FOXP3 KO MT-2 biol rep 3


title: MUC21594 CM-OXT-101-19-G

value: Oxytocin neurons

value: Brain

value: C57BL/6JRj -OXT-sun1-sfGFP

value: 6 months

value: Male


title: MUC21595 CM-OXT-101-19-H

value: SC diet


title: FOXP3 KO MT-2 biol rep 1


title: MUC21592 CM-OXT-101-19-E


title: WT MT-2 biol rep 3

value: WT


title: WT MT-2 biol rep 4


title: MUC21591 CM-OXT-101-19-D


title: MUC21593 CM-OXT-101-19-F


title: WT MT-2 biol rep 2


description: JHH-6 cells transiently transfected with 100 pmol siRNA APE1, replicate 1


title: WT MT-2 biol rep 1


title: MUC21596 CM-OXT-101-19-I

value: HFHS diet


title: Nascent RNA, chromatin-released, pulse-chase time point 10 min

city: Heraklion

first: Evgenia

last: Ntini

address: Nik. Plastira 100

country: Greece

institute: IMBB-FORTH

description:
chromatin fractionation at 0.5 M urea; fragmentation (0.1 M NaOH on ice) prior to 4-SU-label based purification of nascent RNA
NP10

data_processing:
Reads were aligned to GRCh38/hg38 and to ERCC92 sequences using STAR version 2.5.4a.
Only uniquely aligned reads were kept
bigWig files were generated with WigToBigWig from UCSC utilities
Spike-in normalized strand-specific read counts over genomic features were extracted with bedtools coverage -s and normalized to feature length.
Code available under https://github.com/evgnt/chrTT-seq
Assembly: GRCh38/hg38
Supplementary files format and content: bigWig

growth_protocol: MCF-7 cells were maintained in DMEM supplemented with 5 % fetal bovine serum (FBS) in humidified atmosphere with 5% CO2 at 37 °C.

extract_protocol:
Chromatin fractionation was done at 0.5 M urea and RNA was extracted from the chromatin-associated and nucleoplasmic fraction with acidic phenol (pH 4.5) and acidic phenol/chloroform.
RNA libraries for RNA-seq from 4-SU-labeled purified RNA were prepared using the TrueSeq Stranded mRNA Library Preparation Kit (without RiboZero).

treatment_protocol:
Cells were seeded to ~ 80 % confluency and labeled with 1 mM 4-thio-uridine for 8 min.
Cells were washed with PBS and chase was applied with 10 mM uridine for 5, 10, 15, 20 min.

value: breast cancer cells

value: 1mM 4-thiouridine for 8 min, followed by chase with 10 mM uridine

tag: time

value: 10 min chase

tag: molecule subtype

value: chromatin-released, 4-SU-labeled RNA


title: MUC21590 CM-OXT-101-19-C


title: Nascent RNA, chromatin-released, pulse-chase time point 15 min

description:
chromatin fractionation at 0.5 M urea; fragmentation (0.1 M NaOH on ice) prior to 4-SU-label based purification of nascent RNA
NP15

tag: cell line

tag: genotype

value: 15 min chase


title: MUC21588 CM-OXT-101-19-A


title: Nascent RNA, chromatin-released, pulse-chase time point 0 min

description:
chromatin fractionation at 0.5 M urea; fragmentation (0.1 M NaOH on ice) prior to 4-SU-label based purification of nascent RNA
NP0

value: 0 min chase


title: Nascent RNA, chromatin-released, pulse-chase time point 20 min

description:
chromatin fractionation at 0.5 M urea; fragmentation (0.1 M NaOH on ice) prior to 4-SU-based purification of nascent RNA from the chromatin-associated and chromatin-released fraction
NP20

value: 20 min chase


title: Nascent RNA, chromatin-released, pulse-chase time point 5 min

description:
chromatin fractionation at 0.5 M urea; fragmentation (0.1 M NaOH on ice) prior to 4-SU-label based purification of nascent RNA
NP5

value: 5 min chase


title: MUC21589 CM-OXT-101-19-B


title: MUC21586 CM-OXT-101-18-O


title: MUC21587 CM-OXT-101-18-P