title: p1dDec13.10cell 24hr MCSF IFN+TNF 10 G5

source_name: p1dDec13.10cell 24hr MCSF IFN+TNF 10 G5


title: p1dDec13.10cell 24hr MCSF IFN+TNF 10 F8

status: Public on Mar 27 2017

type: RNA

city: New Haven

first: Andrew

last: Martins

state: Connecticut

address: 100 College Street Rm 1155

department: Immunobiology

country: USA

institute: Yale School of Medicine

data_processing: We convert gene expression data exported from Fluidigm Real-time PCR Analysis software from Ct (Cycle threshold) units to the more convenient Et=40-Ct units. To correct for plate-plate effects, we shifted all detected gene measurements in a plate by a plate-specific global factor, which is chosen such that the median ERCC-0003 level of the shifted data across all samples in the plate becomes the same across all plates. We then do sample-level normalization by utilizing the variation of control mRNA levels across samples within/across plates. This sample-to-sample variation in ERCC levels correlated with that of highly expressed genes), suggesting well-to-well differences in the starting amount of mRNA or other such technical factors at play besides independent measurement errors. To correct for this potential technical variation, we fitted a linear regression model (expressed in the notation of statistical environment R as “Gene-expression ~ Plate + ERCC-0002-expression + ERCC-0003-expression + ERCC-0044-expression”) separately in each condition, and took the fit residuals as the normalized log-expression of the gene. Note that this regression model, which allows plate effects to be different for different genes, is fitted to the already plate-level normalized data discussed above where the same plate effect was assumed for all genes.

label: FAM

molecule: total RNA

organism: Homo sapiens

source_name: p1dDec13.10cell 24hr MCSF IFN+TNF 10 F8

label_protocol: Sort plates containing Ten cell RNA were thawed, and 1.35 uL of STA mix (1.25 uL of a pool of 96 Taqman assays at 0.2x concentration, and 0.1 uL Superscript III/Platinum Taq mix (CellsDirect kit, Life Technologies)) were added to each well. Plates were then sealed with clear film (Bio-rad) and centrifuged at 2000x G for 3 minutes and STA was run on a CFX Connect thermal cycler (Bio-rad) with the following cycling parameters: 50°C for 15 mins, 95°C for 2 minutes, then 18 cycles of 95°C for 15 seconds and 60°C for 4 minutes. Plates were then placed on ice and cold 10 mM Tris pH 8.0, 0.1 mM EDTA buffer was added to each well at 20 uL per well. Samples were then analyzed by qPCR on the Fluidigm Biomark instrument using 96.96 chips according to manufacturer’s instructions (Fluidigm). Briefly, 2.25 uL per well of each amplified, diluted single cell sample was mixed with 2.5 uL Ssofast 2X Probes Supermix (Bio-rad) that had 0.5X ROX reference dye added (from the CellsDirect Kit, Life Technologies), and 0.25 uL sample loading reagent (Fluidigm). Taqman assays (20x concentration) were combined 1:1 with Assay loading reagent (Fluidigm). Sample and assay mixes were then added to the wells of the 96.96 chip for gene expression measurement. The normal speed cycling gene expression protocol was used, with 40 cycles of amplification. Data was exported from Fluidigm Real-time PCR Analysis software version 3.1.3, using Linear (Derivative) Baseline method, a global threshold of 0.01, and a 0.65 quality threshold, parameters which were found to exclude non-specific amplification and reduce plate-plate variation.

growth_protocol: Peripheral blood leukapheresis sampleswas obtained from healthy donors. Mononuclear cells were isolated using Ficoll-Paque PLUS (low endotoxin) gradient centrifugation (GE Healthcare Life Sciences). Monocytes were isolated from mononuclear cells using the Dynabeads Untouched Human Monocytes negative isolation kit (Life Technologies), which utilizes biotinylated monoclonal antibodies towards CD3, CD7, CD16a, CD16b, CD19, CD56, CD123, and CD235a, with streptavidin linked magnetic beads for depletion of non-monocyte cells. Isolated monocytes were frozen in 10% DMSO (Sigma), 40% Human AB serum (Gemini Biosciences) and 50% X-vivo 15 media (Lonza) by the following procedure: isolated monocytes obtained using the Dynabeads isolation kit were resuspended at 20 million/mL in 4°C X-vivo 15 media (Phenol red and gentamycin free), and an equal volume of filtered, 4°C 20%DMSO/80% Human AB serum was added dropwise while mixing the cell suspension. Cells were immediately transferred to cyrovials and frozen in a Nalgene Mr. Frosty cell freezing container (Thermo Scientific) in a -80 freezer for 24 hours, followed by transfer to liquid nitrogen storage. For use, frozen monocyte vials were quickly thawed in a 37°C water bath and resuspended in X-vivo 15 media (phenol red and gentamycin free) that had been pre-equilibrated in a 37°C, 5% CO2 incubator. Cells were plated on tissue-culture treated 6-well dishes (Corning) at 1 million cells/mL in 2 mL per well. Human macrophage-colony stimulating factor (M-CSF, Peprotech or R&D systems) was added to the media at a concentration of 100 ng/mL. On day 3 and day 6 of differentiation, all culture media was removed from the dish and replaced by fresh, 37°C, 5% CO2 incubator equilibrated X-vivo 15 containing 100 ng/mL M-CSF.

extract_protocol: Harvested macrophages were incubated on ice in PBS containing 5% human AB serum for 15 minutes, followed by surface antibody staining for 30 minutes on ice, in the same buffer, with the following antibody cocktail: FITC anti-CD36, clone 5-271 (Biolegend); PE anti-CD163, clone GHI/61 (Biolegend); PerCP anti-CD16, clone 3G8 (Biolegend); PE-Cy7 anti-CD197, clone G043H7 (Biolegend); APC-Cy7 anti-CD206, clone 15-2 (Biolegend); Alexa Fluor 700 anti-CD195, clone HEK/1/85a (Biolegend); Alexa Fluor 647 anti-CD192, clone TG5/CCR2 (Biolegend); Pacific Orange anti-CD14, clone TüK4 (Life Technologies). Following staining, cells were washed once with ice cold PBS, and resuspended in ice cold PBS containing a 1 in 1000 dilution of Pacific Blue Fixable Live/Dead stain (Life Technologies), which is a fluorescent dye reactive with cellular amines. When the cell membrane becomes permeable, the dye gains access to intracellular amines and hence the fluorescence of the cell increases. For sorting, cells were gated upon Forward scatter (FSC)-width versus side scatter(SSC)-area for doublet discrimination, followed by gating on Pacific blue low (membrane intact) cells (versus FSC-area). A 100 μm nozzle was used at 20 PSI pressure and a drop drive frequency of 34.5 kHz. Sorting was performed on a FACSAriaII (Becton Dickinson) and FACSDiva version 6.1.3 software using Single cell precision mode with default parameters (Yield mask = 0, Purity mask = 32, Phase mask = 16). Sort alignment was checked immediately before sorting, by sorting beads onto a covered PCR plate and confirming that the droplets land in the center of several different wells across the plate. Cells (ten cells per well) were deposited into individual wells of a low-profile 96 well PCR plate (Bio-rad) containing 2.5 uL CellsDirect 2x Reaction Mix (Life Technologies), 0.05 uL Ambion SUPERase-In RNAse inhibitor (Life technologies), and 1 uL RNAse-free water. Cells from different treatment groups were sorted into alternating wells across the plate, to avoid positional or “edge” effects across the plate. Each plate contained a no template control well, and a seven point dilution of a pooled RNA sample derived from a combination of RNA isolated from non-treated, IFNγ, and IL-4 treated macrophages, as a reference positive control. Control RNA was isolated using the miRNeasy mini kit (Qiagen). All wells except the positive control wells also contained ERCC RNA reference standards (Life Technologies) spiked in at 1/50,000 final dilution from the stock ERCC mixture.

treatment_protocol: Fresh media on day 6 was also supplemented with 100 ng/mL Interferon (IFN)γ and 100 ng/mL Tumor necrosis factor α (IFNγ and TNFα treated cells), 100 ng/mL Interleukin 4 (IL-4 treated cells), 10 ng/mL IL-10 (IL-10 treated cells) (cytokines from Peprotech), or M-CSF alone (Control). Cells were harvested for analysis 24 hours after treatment.

tag: plateid

tag: treatment time

tag: growth factor

value: MCSF

tag: treatment

value: IFN+TNF

tag: cell number

tag: well


title: p1dDec13.10cell 24hr MCSF IFN+TNF 10 F4

source_name: p1dDec13.10cell 24hr MCSF IFN+TNF 10 F4


title: p1dDec13.10cell 24hr MCSF IL10 10 E5

source_name: p1dDec13.10cell 24hr MCSF IL10 10 E5


title: p1dDec13.10cell 24hr MCSF IFN+TNF 10 E7

source_name: p1dDec13.10cell 24hr MCSF IFN+TNF 10 E7


title: p1dDec13.10cell 24hr MCSF IFN+TNF 10 E3

source_name: p1dDec13.10cell 24hr MCSF IFN+TNF 10 E3


title: p1dDec13.10cell 24hr MCSF IL10 10 B2

source_name: p1dDec13.10cell 24hr MCSF IL10 10 B2


title: p1dDec13.10cell 24hr MCSF IFN+TNF 10 F12


title: p1dDec13.10cell 24hr MCSF IFN+TNF 10 D10


title: p1dDec13.10cell 24hr MCSF IFN+TNF 10 C5

source_name: p1dDec13.10cell 24hr MCSF IFN+TNF 10 C5


title: p1dDec13.10cell 24hr MCSF IFN+TNF 10 C9

source_name: p1dDec13.10cell 24hr MCSF IFN+TNF 10 C9


title: p1dDec13.10cell 24hr MCSF IFN+TNF 10 D6

source_name: p1dDec13.10cell 24hr MCSF IFN+TNF 10 D6


title: p1dDec13.10cell 24hr MCSF IFN+TNF 10 E11


title: p1dDec13.10cell 24hr MCSF IFN+TNF 10 A11


title: p1dDec13.10cell 24hr MCSF CNT 10 H9

source_name: p1dDec13.10cell 24hr MCSF CNT 10 H9

value: CNT


title: p1dDec13.10cell 24hr MCSF IFN+TNF 10 B4

source_name: p1dDec13.10cell 24hr MCSF IFN+TNF 10 B4


title: p1dDec13.10cell 24hr MCSF CNT 10 H5

source_name: p1dDec13.10cell 24hr MCSF CNT 10 H5


title: p1dDec13.10cell 24hr MCSF CNT 10 F11

source_name: p1dDec13.10cell 24hr MCSF CNT 10 F11


title: p1dDec13.10cell 24hr MCSF IFN+TNF 10 B12


title: p1dDec13.10cell 24hr MCSF CNT 10 E6

source_name: p1dDec13.10cell 24hr MCSF CNT 10 E6


title: p1dDec13.10cell 24hr MCSF CNT 10 G12

source_name: p1dDec13.10cell 24hr MCSF CNT 10 G12


title: p1dDec13.10cell 24hr MCSF IFN+TNF 10 A7

source_name: p1dDec13.10cell 24hr MCSF IFN+TNF 10 A7


title: p1dDec13.10cell 24hr MCSF CNT 10 F7

source_name: p1dDec13.10cell 24hr MCSF CNT 10 F7


title: p1dDec13.10cell 24hr MCSF CNT 10 F3

source_name: p1dDec13.10cell 24hr MCSF CNT 10 F3


title: p1dDec13.10cell 24hr MCSF CNT 10 G8

source_name: p1dDec13.10cell 24hr MCSF CNT 10 G8


title: p1dDec13.10cell 24hr MCSF CNT 10 E10

source_name: p1dDec13.10cell 24hr MCSF CNT 10 E10


title: p1dDec13.10cell 24hr MCSF CNT 10 E2

source_name: p1dDec13.10cell 24hr MCSF CNT 10 E2


title: p1dDec13.10cell 24hr MCSF CNT 10 D9

source_name: p1dDec13.10cell 24hr MCSF CNT 10 D9


title: p1dDec13.10cell 24hr MCSF IFN+TNF 10 A3

source_name: p1dDec13.10cell 24hr MCSF IFN+TNF 10 A3


title: p1dDec13.10cell 24hr MCSF CNT 10 G4

source_name: p1dDec13.10cell 24hr MCSF CNT 10 G4


title: p1dDec13.10cell 24hr MCSF CNT 10 D5

source_name: p1dDec13.10cell 24hr MCSF CNT 10 D5


title: p2dDec13.10cell 24hr MCSF IFN+TNF 10 E11


title: p1dDec13.10cell 24hr MCSF IFN+TNF 10 B8

source_name: p1dDec13.10cell 24hr MCSF IFN+TNF 10 B8


title: p1dDec13.10cell 24hr MCSF CNT 10 C12

source_name: p1dDec13.10cell 24hr MCSF CNT 10 C12


title: p1dDec13.10cell 24hr MCSF CNT 10 C8

source_name: p1dDec13.10cell 24hr MCSF CNT 10 C8


title: p1dDec13.10cell 24hr MCSF CNT 10 B3

source_name: p1dDec13.10cell 24hr MCSF CNT 10 B3


title: p1dDec13.10cell 24hr MCSF CNT 10 B7

source_name: p1dDec13.10cell 24hr MCSF CNT 10 B7


title: p1dDec13.10cell 24hr MCSF CNT 10 A2

source_name: p1dDec13.10cell 24hr MCSF CNT 10 A2


title: p1dDec13.10cell 24hr MCSF CNT 10 A6

source_name: p1dDec13.10cell 24hr MCSF CNT 10 A6


title: p1dDec13.10cell 24hr MCSF CNT 10 A10

source_name: p1dDec13.10cell 24hr MCSF CNT 10 A10


title: p4dJul23.10cell 24hr MCSF IL4 10 H3

source_name: p4dJul23.10cell 24hr MCSF IL4 10 H3


title: p4dJul23.10cell 24hr MCSF IL4 10 H7

source_name: p4dJul23.10cell 24hr MCSF IL4 10 H7


title: p4dJul23.10cell 24hr MCSF IL4 10 H11

source_name: p4dJul23.10cell 24hr MCSF IL4 10 H11


title: p4dJul23.10cell 24hr MCSF IL4 10 G6

source_name: p4dJul23.10cell 24hr MCSF IL4 10 G6


title: p1dDec13.10cell 24hr MCSF CNT 10 C4

source_name: p1dDec13.10cell 24hr MCSF CNT 10 C4


title: p4dJul23.10cell 24hr MCSF IL4 10 D7

source_name: p4dJul23.10cell 24hr MCSF IL4 10 D7


title: p4dJul23.10cell 24hr MCSF IL4 10 D3

source_name: p4dJul23.10cell 24hr MCSF IL4 10 D3


title: p4dJul23.10cell 24hr MCSF IL4 10 E4

source_name: p4dJul23.10cell 24hr MCSF IL4 10 E4


title: p4dJul23.10cell 24hr MCSF IL4 10 E12

source_name: p4dJul23.10cell 24hr MCSF IL4 10 E12


title: p4dJul23.10cell 24hr MCSF IL4 10 D11

source_name: p4dJul23.10cell 24hr MCSF IL4 10 D11


title: p4dJul23.10cell 24hr MCSF IL4 10 E8

source_name: p4dJul23.10cell 24hr MCSF IL4 10 E8


title: p4dJul23.10cell 24hr MCSF IL4 10 F5

source_name: p4dJul23.10cell 24hr MCSF IL4 10 F5


title: p4dJul23.10cell 24hr MCSF IL4 10 F9

source_name: p4dJul23.10cell 24hr MCSF IL4 10 F9


title: p4dJul23.10cell 24hr MCSF IL4 10 C6

source_name: p4dJul23.10cell 24hr MCSF IL4 10 C6


title: p4dJul23.10cell 24hr MCSF IL4 10 C10

source_name: p4dJul23.10cell 24hr MCSF IL4 10 C10


title: p1dDec13.10cell 24hr MCSF CNT 10 B11

source_name: p1dDec13.10cell 24hr MCSF CNT 10 B11


title: p4dJul23.10cell 24hr MCSF IL4 10 B9

source_name: p4dJul23.10cell 24hr MCSF IL4 10 B9


title: p4dJul23.10cell 24hr MCSF IL4 10 B5

source_name: p4dJul23.10cell 24hr MCSF IL4 10 B5


title: p4dJul23.10cell 24hr MCSF IL4 10 G2

source_name: p4dJul23.10cell 24hr MCSF IL4 10 G2


title: p4dJul23.10cell 24hr MCSF IL4 10 G10

source_name: p4dJul23.10cell 24hr MCSF IL4 10 G10


title: p4dJul23.10cell 24hr MCSF IL4 10 A12

source_name: p4dJul23.10cell 24hr MCSF IL4 10 A12


title: p4dJul23.10cell 24hr MCSF IL10 10 H6

source_name: p4dJul23.10cell 24hr MCSF IL10 10 H6


title: p4dJul23.10cell 24hr MCSF IL10 10 H2

source_name: p4dJul23.10cell 24hr MCSF IL10 10 H2


title: p4dJul23.10cell 24hr MCSF IL4 10 A4

source_name: p4dJul23.10cell 24hr MCSF IL4 10 A4


title: p4dJul23.10cell 24hr MCSF IL10 10 H10

source_name: p4dJul23.10cell 24hr MCSF IL10 10 H10


title: p4dJul23.10cell 24hr MCSF IL10 10 G3

source_name: p4dJul23.10cell 24hr MCSF IL10 10 G3


title: p4dJul23.10cell 24hr MCSF IL10 10 F8

source_name: p4dJul23.10cell 24hr MCSF IL10 10 F8


title: p4dJul23.10cell 24hr MCSF IL10 10 G11

source_name: p4dJul23.10cell 24hr MCSF IL10 10 G11


title: p4dJul23.10cell 24hr MCSF IL10 10 F4

source_name: p4dJul23.10cell 24hr MCSF IL10 10 F4


title: p4dJul23.10cell 24hr MCSF IL10 10 D10

source_name: p4dJul23.10cell 24hr MCSF IL10 10 D10


title: p4dJul23.10cell 24hr MCSF IL10 10 D2

source_name: p4dJul23.10cell 24hr MCSF IL10 10 D2


title: p4dJul23.10cell 24hr MCSF IL4 10 A8

source_name: p4dJul23.10cell 24hr MCSF IL4 10 A8


title: p4dJul23.10cell 24hr MCSF IL10 10 D6

source_name: p4dJul23.10cell 24hr MCSF IL10 10 D6


title: p4dJul23.10cell 24hr MCSF IL10 10 E5

source_name: p4dJul23.10cell 24hr MCSF IL10 10 E5


title: p4dJul23.10cell 24hr MCSF IL10 10 C3

source_name: p4dJul23.10cell 24hr MCSF IL10 10 C3


title: p4dJul23.10cell 24hr MCSF IL10 10 E9

source_name: p4dJul23.10cell 24hr MCSF IL10 10 E9


title: p4dJul23.10cell 24hr MCSF IL10 10 C7

source_name: p4dJul23.10cell 24hr MCSF IL10 10 C7


title: p4dJul23.10cell 24hr MCSF IL10 10 C11

source_name: p4dJul23.10cell 24hr MCSF IL10 10 C11


title: p4dJul23.10cell 24hr MCSF IL10 10 B8

source_name: p4dJul23.10cell 24hr MCSF IL10 10 B8


title: p4dJul23.10cell 24hr MCSF IL10 10 G7

source_name: p4dJul23.10cell 24hr MCSF IL10 10 G7


title: p4dJul23.10cell 24hr MCSF IL10 10 B4

source_name: p4dJul23.10cell 24hr MCSF IL10 10 B4


title: p4dJul23.10cell 24hr MCSF IL10 10 B12

source_name: p4dJul23.10cell 24hr MCSF IL10 10 B12


title: p4dJul23.10cell 24hr MCSF IFN+TNF 10 H4

source_name: p4dJul23.10cell 24hr MCSF IFN+TNF 10 H4


title: p4dJul23.10cell 24hr MCSF IFN+TNF 10 H8

source_name: p4dJul23.10cell 24hr MCSF IFN+TNF 10 H8


title: p4dJul23.10cell 24hr MCSF IFN+TNF 10 H12


title: p4dJul23.10cell 24hr MCSF IL10 10 A5

source_name: p4dJul23.10cell 24hr MCSF IL10 10 A5


title: p4dJul23.10cell 24hr MCSF IL10 10 A9

source_name: p4dJul23.10cell 24hr MCSF IL10 10 A9


title: p4dJul23.10cell 24hr MCSF IL4 10 C2

source_name: p4dJul23.10cell 24hr MCSF IL4 10 C2


title: p4dJul23.10cell 24hr MCSF IFN+TNF 10 G9

source_name: p4dJul23.10cell 24hr MCSF IFN+TNF 10 G9


title: p4dJul23.10cell 24hr MCSF IFN+TNF 10 G5

source_name: p4dJul23.10cell 24hr MCSF IFN+TNF 10 G5


title: p4dJul23.10cell 24hr MCSF IFN+TNF 10 F2

source_name: p4dJul23.10cell 24hr MCSF IFN+TNF 10 F2


title: p4dJul23.10cell 24hr MCSF IFN+TNF 10 F6

source_name: p4dJul23.10cell 24hr MCSF IFN+TNF 10 F6


title: p4dJul23.10cell 24hr MCSF IFN+TNF 10 F10


title: p4dJul23.10cell 24hr MCSF IL10 10 F12

source_name: p4dJul23.10cell 24hr MCSF IL10 10 F12


title: p4dJul23.10cell 24hr MCSF IFN+TNF 10 E7

source_name: p4dJul23.10cell 24hr MCSF IFN+TNF 10 E7


title: p4dJul23.10cell 24hr MCSF IFN+TNF 10 E11


title: p4dJul23.10cell 24hr MCSF IFN+TNF 10 D12


title: p4dJul23.10cell 24hr MCSF IFN+TNF 10 C5

source_name: p4dJul23.10cell 24hr MCSF IFN+TNF 10 C5


title: p4dJul23.10cell 24hr MCSF IFN+TNF 10 D4

source_name: p4dJul23.10cell 24hr MCSF IFN+TNF 10 D4


title: p4dJul23.10cell 24hr MCSF IFN+TNF 10 C9

source_name: p4dJul23.10cell 24hr MCSF IFN+TNF 10 C9


title: p4dJul23.10cell 24hr MCSF CNT 10 H5

source_name: p4dJul23.10cell 24hr MCSF CNT 10 H5


title: p4dJul23.10cell 24hr MCSF CNT 10 H9

source_name: p4dJul23.10cell 24hr MCSF CNT 10 H9


title: p4dJul23.10cell 24hr MCSF IFN+TNF 10 A11


title: p4dJul23.10cell 24hr MCSF IFN+TNF 10 A3

source_name: p4dJul23.10cell 24hr MCSF IFN+TNF 10 A3


title: p4dJul23.10cell 24hr MCSF CNT 10 G4

source_name: p4dJul23.10cell 24hr MCSF CNT 10 G4


title: p4dJul23.10cell 24hr MCSF CNT 10 F11

source_name: p4dJul23.10cell 24hr MCSF CNT 10 F11


title: p4dJul23.10cell 24hr MCSF IFN+TNF 10 B2

source_name: p4dJul23.10cell 24hr MCSF IFN+TNF 10 B2


title: p4dJul23.10cell 24hr MCSF CNT 10 E6

source_name: p4dJul23.10cell 24hr MCSF CNT 10 E6


title: p4dJul23.10cell 24hr MCSF IFN+TNF 10 B10


title: p4dJul23.10cell 24hr MCSF CNT 10 G8

source_name: p4dJul23.10cell 24hr MCSF CNT 10 G8


title: p4dJul23.10cell 24hr MCSF IFN+TNF 10 A7

source_name: p4dJul23.10cell 24hr MCSF IFN+TNF 10 A7


title: p4dJul23.10cell 24hr MCSF IFN+TNF 10 E3

source_name: p4dJul23.10cell 24hr MCSF IFN+TNF 10 E3


title: p4dJul23.10cell 24hr MCSF IFN+TNF 10 B6

source_name: p4dJul23.10cell 24hr MCSF IFN+TNF 10 B6


title: p4dJul23.10cell 24hr MCSF IFN+TNF 10 D8

source_name: p4dJul23.10cell 24hr MCSF IFN+TNF 10 D8


title: p4dJul23.10cell 24hr MCSF CNT 10 F3

source_name: p4dJul23.10cell 24hr MCSF CNT 10 F3


title: p4dJul23.10cell 24hr MCSF CNT 10 E10

source_name: p4dJul23.10cell 24hr MCSF CNT 10 E10


title: p4dJul23.10cell 24hr MCSF CNT 10 F7

source_name: p4dJul23.10cell 24hr MCSF CNT 10 F7


title: p4dJul23.10cell 24hr MCSF CNT 10 E2

source_name: p4dJul23.10cell 24hr MCSF CNT 10 E2


title: p4dJul23.10cell 24hr MCSF CNT 10 D9

source_name: p4dJul23.10cell 24hr MCSF CNT 10 D9


title: p1dDec13.10cell 24hr MCSF IL10 10 H8

source_name: p1dDec13.10cell 24hr MCSF IL10 10 H8


title: p4dJul23.10cell 24hr MCSF CNT 10 C8

source_name: p4dJul23.10cell 24hr MCSF CNT 10 C8


title: p4dJul23.10cell 24hr MCSF CNT 10 D5

source_name: p4dJul23.10cell 24hr MCSF CNT 10 D5


title: p4dJul23.10cell 24hr MCSF CNT 10 C4

source_name: p4dJul23.10cell 24hr MCSF CNT 10 C4


title: p4dJul23.10cell 24hr MCSF CNT 10 G12

source_name: p4dJul23.10cell 24hr MCSF CNT 10 G12


title: p4dJul23.10cell 24hr MCSF CNT 10 A2

source_name: p4dJul23.10cell 24hr MCSF CNT 10 A2


title: p4dJul23.10cell 24hr MCSF CNT 10 B11

source_name: p4dJul23.10cell 24hr MCSF CNT 10 B11


title: p4dJul23.10cell 24hr MCSF CNT 10 B7

source_name: p4dJul23.10cell 24hr MCSF CNT 10 B7


title: p4dJul23.10cell 24hr MCSF CNT 10 C12

source_name: p4dJul23.10cell 24hr MCSF CNT 10 C12


title: p3dJul23.10cell 24hr MCSF IL4 10 H3

source_name: p3dJul23.10cell 24hr MCSF IL4 10 H3


title: p3dJul23.10cell 24hr MCSF IL4 10 H7

source_name: p3dJul23.10cell 24hr MCSF IL4 10 H7


title: p3dJul23.10cell 24hr MCSF IL4 10 F9

source_name: p3dJul23.10cell 24hr MCSF IL4 10 F9


title: p3dJul23.10cell 24hr MCSF IL4 10 F5

source_name: p3dJul23.10cell 24hr MCSF IL4 10 F5


title: p3dJul23.10cell 24hr MCSF IL4 10 D7

source_name: p3dJul23.10cell 24hr MCSF IL4 10 D7


title: p3dJul23.10cell 24hr MCSF IL4 10 G6

source_name: p3dJul23.10cell 24hr MCSF IL4 10 G6


title: p3dJul23.10cell 24hr MCSF IL4 10 B9

source_name: p3dJul23.10cell 24hr MCSF IL4 10 B9


title: p3dJul23.10cell 24hr MCSF IL4 10 C10

source_name: p3dJul23.10cell 24hr MCSF IL4 10 C10


title: p3dJul23.10cell 24hr MCSF IL4 10 H11

source_name: p3dJul23.10cell 24hr MCSF IL4 10 H11


title: p3dJul23.10cell 24hr MCSF IL4 10 G2

source_name: p3dJul23.10cell 24hr MCSF IL4 10 G2


title: p3dJul23.10cell 24hr MCSF IL4 10 G10

source_name: p3dJul23.10cell 24hr MCSF IL4 10 G10


title: p3dJul23.10cell 24hr MCSF IL4 10 D3

source_name: p3dJul23.10cell 24hr MCSF IL4 10 D3


title: p3dJul23.10cell 24hr MCSF IL4 10 D11

source_name: p3dJul23.10cell 24hr MCSF IL4 10 D11


title: p3dJul23.10cell 24hr MCSF IL4 10 C2

source_name: p3dJul23.10cell 24hr MCSF IL4 10 C2


title: p3dJul23.10cell 24hr MCSF IL4 10 E8

source_name: p3dJul23.10cell 24hr MCSF IL4 10 E8


title: p4dJul23.10cell 24hr MCSF CNT 10 A10

source_name: p4dJul23.10cell 24hr MCSF CNT 10 A10


title: p3dJul23.10cell 24hr MCSF IL4 10 A8

source_name: p3dJul23.10cell 24hr MCSF IL4 10 A8


title: p3dJul23.10cell 24hr MCSF IL4 10 C6

source_name: p3dJul23.10cell 24hr MCSF IL4 10 C6


title: p4dJul23.10cell 24hr MCSF CNT 10 B3

source_name: p4dJul23.10cell 24hr MCSF CNT 10 B3


title: p4dJul23.10cell 24hr MCSF CNT 10 A6

source_name: p4dJul23.10cell 24hr MCSF CNT 10 A6


title: p3dJul23.10cell 24hr MCSF IL10 10 H6

source_name: p3dJul23.10cell 24hr MCSF IL10 10 H6


title: p3dJul23.10cell 24hr MCSF IL4 10 A12

source_name: p3dJul23.10cell 24hr MCSF IL4 10 A12


title: p3dJul23.10cell 24hr MCSF IL4 10 E4

source_name: p3dJul23.10cell 24hr MCSF IL4 10 E4


title: p3dJul23.10cell 24hr MCSF IL4 10 B5

source_name: p3dJul23.10cell 24hr MCSF IL4 10 B5


title: p3dJul23.10cell 24hr MCSF IL10 10 H10

source_name: p3dJul23.10cell 24hr MCSF IL10 10 H10


title: p3dJul23.10cell 24hr MCSF IL10 10 G3

source_name: p3dJul23.10cell 24hr MCSF IL10 10 G3


title: p3dJul23.10cell 24hr MCSF IL10 10 H2

source_name: p3dJul23.10cell 24hr MCSF IL10 10 H2


title: p3dJul23.10cell 24hr MCSF IL10 10 G7

source_name: p3dJul23.10cell 24hr MCSF IL10 10 G7


title: p3dJul23.10cell 24hr MCSF IL10 10 G11

source_name: p3dJul23.10cell 24hr MCSF IL10 10 G11


title: p3dJul23.10cell 24hr MCSF IL10 10 F12

source_name: p3dJul23.10cell 24hr MCSF IL10 10 F12


title: p3dJul23.10cell 24hr MCSF IL10 10 F4

source_name: p3dJul23.10cell 24hr MCSF IL10 10 F4


title: p3dJul23.10cell 24hr MCSF IL10 10 E5

source_name: p3dJul23.10cell 24hr MCSF IL10 10 E5


title: p3dJul23.10cell 24hr MCSF IL10 10 E9

source_name: p3dJul23.10cell 24hr MCSF IL10 10 E9


title: p3dJul23.10cell 24hr MCSF IL4 10 A4

source_name: p3dJul23.10cell 24hr MCSF IL4 10 A4


title: p3dJul23.10cell 24hr MCSF IL10 10 F8

source_name: p3dJul23.10cell 24hr MCSF IL10 10 F8


title: p3dJul23.10cell 24hr MCSF IL10 10 B12

source_name: p3dJul23.10cell 24hr MCSF IL10 10 B12


title: p3dJul23.10cell 24hr MCSF IL10 10 D6

source_name: p3dJul23.10cell 24hr MCSF IL10 10 D6


title: p3dJul23.10cell 24hr MCSF IL10 10 A9

source_name: p3dJul23.10cell 24hr MCSF IL10 10 A9


title: p3dJul23.10cell 24hr MCSF IL10 10 C11

source_name: p3dJul23.10cell 24hr MCSF IL10 10 C11


title: p3dJul23.10cell 24hr MCSF IL10 10 B4

source_name: p3dJul23.10cell 24hr MCSF IL10 10 B4


title: p3dJul23.10cell 24hr MCSF IL10 10 B8

source_name: p3dJul23.10cell 24hr MCSF IL10 10 B8


title: p3dJul23.10cell 24hr MCSF IL10 10 C3

source_name: p3dJul23.10cell 24hr MCSF IL10 10 C3


title: p3dJul23.10cell 24hr MCSF IL10 10 C7

source_name: p3dJul23.10cell 24hr MCSF IL10 10 C7


title: p3dJul23.10cell 24hr MCSF IL10 10 D10

source_name: p3dJul23.10cell 24hr MCSF IL10 10 D10


title: p3dJul23.10cell 24hr MCSF IFN+TNF 10 H8

source_name: p3dJul23.10cell 24hr MCSF IFN+TNF 10 H8


title: p3dJul23.10cell 24hr MCSF IL10 10 D2

source_name: p3dJul23.10cell 24hr MCSF IL10 10 D2


title: p3dJul23.10cell 24hr MCSF IFN+TNF 10 F6

source_name: p3dJul23.10cell 24hr MCSF IFN+TNF 10 F6


title: p3dJul23.10cell 24hr MCSF IFN+TNF 10 G5

source_name: p3dJul23.10cell 24hr MCSF IFN+TNF 10 G5


title: p3dJul23.10cell 24hr MCSF IFN+TNF 10 G9

source_name: p3dJul23.10cell 24hr MCSF IFN+TNF 10 G9


title: p3dJul23.10cell 24hr MCSF IFN+TNF 10 E7

source_name: p3dJul23.10cell 24hr MCSF IFN+TNF 10 E7


title: p3dJul23.10cell 24hr MCSF IFN+TNF 10 F10


title: p3dJul23.10cell 24hr MCSF IFN+TNF 10 F2

source_name: p3dJul23.10cell 24hr MCSF IFN+TNF 10 F2


title: p3dJul23.10cell 24hr MCSF IFN+TNF 10 H4

source_name: p3dJul23.10cell 24hr MCSF IFN+TNF 10 H4


title: p3dJul23.10cell 24hr MCSF IFN+TNF 10 E11


title: p3dJul23.10cell 24hr MCSF IFN+TNF 10 D12


title: p3dJul23.10cell 24hr MCSF IFN+TNF 10 C9

source_name: p3dJul23.10cell 24hr MCSF IFN+TNF 10 C9


title: p3dJul23.10cell 24hr MCSF IFN+TNF 10 H12


title: p3dJul23.10cell 24hr MCSF IFN+TNF 10 D4

source_name: p3dJul23.10cell 24hr MCSF IFN+TNF 10 D4


title: p3dJul23.10cell 24hr MCSF IFN+TNF 10 C5

source_name: p3dJul23.10cell 24hr MCSF IFN+TNF 10 C5


title: p3dJul23.10cell 24hr MCSF IFN+TNF 10 E3

source_name: p3dJul23.10cell 24hr MCSF IFN+TNF 10 E3


title: p3dJul23.10cell 24hr MCSF IFN+TNF 10 B6

source_name: p3dJul23.10cell 24hr MCSF IFN+TNF 10 B6


title: p3dJul23.10cell 24hr MCSF CNT 10 H5

source_name: p3dJul23.10cell 24hr MCSF CNT 10 H5


title: p3dJul23.10cell 24hr MCSF IFN+TNF 10 B2

source_name: p3dJul23.10cell 24hr MCSF IFN+TNF 10 B2


title: p3dJul23.10cell 24hr MCSF CNT 10 G8

source_name: p3dJul23.10cell 24hr MCSF CNT 10 G8


title: p3dJul23.10cell 24hr MCSF IFN+TNF 10 A3

source_name: p3dJul23.10cell 24hr MCSF IFN+TNF 10 A3


title: p3dJul23.10cell 24hr MCSF CNT 10 H9

source_name: p3dJul23.10cell 24hr MCSF CNT 10 H9


title: p3dJul23.10cell 24hr MCSF IFN+TNF 10 A11


title: p3dJul23.10cell 24hr MCSF CNT 10 G4

source_name: p3dJul23.10cell 24hr MCSF CNT 10 G4


title: p3dJul23.10cell 24hr MCSF IFN+TNF 10 D8

source_name: p3dJul23.10cell 24hr MCSF IFN+TNF 10 D8


title: p3dJul23.10cell 24hr MCSF CNT 10 F7

source_name: p3dJul23.10cell 24hr MCSF CNT 10 F7


title: p3dJul23.10cell 24hr MCSF CNT 10 F3

source_name: p3dJul23.10cell 24hr MCSF CNT 10 F3


title: p3dJul23.10cell 24hr MCSF CNT 10 F11

source_name: p3dJul23.10cell 24hr MCSF CNT 10 F11


title: p3dJul23.10cell 24hr MCSF IFN+TNF 10 A7

source_name: p3dJul23.10cell 24hr MCSF IFN+TNF 10 A7


title: p3dJul23.10cell 24hr MCSF CNT 10 D5

source_name: p3dJul23.10cell 24hr MCSF CNT 10 D5


title: p3dJul23.10cell 24hr MCSF CNT 10 C8

source_name: p3dJul23.10cell 24hr MCSF CNT 10 C8


title: p3dJul23.10cell 24hr MCSF CNT 10 C4

source_name: p3dJul23.10cell 24hr MCSF CNT 10 C4


title: p3dJul23.10cell 24hr MCSF IL10 10 A5

source_name: p3dJul23.10cell 24hr MCSF IL10 10 A5


title: p3dJul23.10cell 24hr MCSF CNT 10 A6

source_name: p3dJul23.10cell 24hr MCSF CNT 10 A6


title: p3dJul23.10cell 24hr MCSF CNT 10 D9

source_name: p3dJul23.10cell 24hr MCSF CNT 10 D9


title: p3dJul23.10cell 24hr MCSF IFN+TNF 10 B10


title: p3dJul23.10cell 24hr MCSF CNT 10 E6

source_name: p3dJul23.10cell 24hr MCSF CNT 10 E6


title: p3dJul23.10cell 24hr MCSF CNT 10 B3

source_name: p3dJul23.10cell 24hr MCSF CNT 10 B3


title: p3dJul23.10cell 24hr MCSF CNT 10 E10

source_name: p3dJul23.10cell 24hr MCSF CNT 10 E10


title: p3dJul23.10cell 24hr MCSF CNT 10 A10

source_name: p3dJul23.10cell 24hr MCSF CNT 10 A10


title: p3dJul23.10cell 24hr MCSF CNT 10 A2

source_name: p3dJul23.10cell 24hr MCSF CNT 10 A2


title: p2dJul23.10cell 24hr MCSF IL4 10 H7

source_name: p2dJul23.10cell 24hr MCSF IL4 10 H7


title: p3dJul23.10cell 24hr MCSF CNT 10 B7

source_name: p3dJul23.10cell 24hr MCSF CNT 10 B7


title: p3dJul23.10cell 24hr MCSF CNT 10 E2

source_name: p3dJul23.10cell 24hr MCSF CNT 10 E2


title: p2dJul23.10cell 24hr MCSF IL4 10 H3

source_name: p2dJul23.10cell 24hr MCSF IL4 10 H3


title: p2dJul23.10cell 24hr MCSF IL4 10 G6

source_name: p2dJul23.10cell 24hr MCSF IL4 10 G6


title: p3dJul23.10cell 24hr MCSF CNT 10 C12

source_name: p3dJul23.10cell 24hr MCSF CNT 10 C12


title: p2dJul23.10cell 24hr MCSF IL4 10 H11

source_name: p2dJul23.10cell 24hr MCSF IL4 10 H11


title: p2dJul23.10cell 24hr MCSF IL4 10 D7

source_name: p2dJul23.10cell 24hr MCSF IL4 10 D7


title: p2dJul23.10cell 24hr MCSF IL4 10 G2

source_name: p2dJul23.10cell 24hr MCSF IL4 10 G2


title: p2dJul23.10cell 24hr MCSF IL4 10 E12

source_name: p2dJul23.10cell 24hr MCSF IL4 10 E12


title: p2dJul23.10cell 24hr MCSF IL4 10 G10

source_name: p2dJul23.10cell 24hr MCSF IL4 10 G10


title: p3dJul23.10cell 24hr MCSF CNT 10 G12

source_name: p3dJul23.10cell 24hr MCSF CNT 10 G12


title: p2dJul23.10cell 24hr MCSF IL4 10 E4

source_name: p2dJul23.10cell 24hr MCSF IL4 10 E4


title: p2dJul23.10cell 24hr MCSF IL4 10 E8

source_name: p2dJul23.10cell 24hr MCSF IL4 10 E8


title: p2dJul23.10cell 24hr MCSF IL4 10 D3

source_name: p2dJul23.10cell 24hr MCSF IL4 10 D3


title: p2dJul23.10cell 24hr MCSF IL4 10 F9

source_name: p2dJul23.10cell 24hr MCSF IL4 10 F9


title: p2dJul23.10cell 24hr MCSF IL4 10 F5

source_name: p2dJul23.10cell 24hr MCSF IL4 10 F5


title: p2dJul23.10cell 24hr MCSF IL4 10 C10

source_name: p2dJul23.10cell 24hr MCSF IL4 10 C10


title: p2dJul23.10cell 24hr MCSF IL4 10 C2

source_name: p2dJul23.10cell 24hr MCSF IL4 10 C2


title: p2dJul23.10cell 24hr MCSF IL4 10 A8

source_name: p2dJul23.10cell 24hr MCSF IL4 10 A8


title: p2dJul23.10cell 24hr MCSF IL4 10 B5

source_name: p2dJul23.10cell 24hr MCSF IL4 10 B5


title: p2dJul23.10cell 24hr MCSF IL4 10 C6

source_name: p2dJul23.10cell 24hr MCSF IL4 10 C6


title: p2dJul23.10cell 24hr MCSF IL4 10 B9

source_name: p2dJul23.10cell 24hr MCSF IL4 10 B9


title: p2dJul23.10cell 24hr MCSF IL4 10 A12

source_name: p2dJul23.10cell 24hr MCSF IL4 10 A12


title: p2dJul23.10cell 24hr MCSF IL10 10 H6

source_name: p2dJul23.10cell 24hr MCSF IL10 10 H6


title: p2dJul23.10cell 24hr MCSF IL4 10 A4

source_name: p2dJul23.10cell 24hr MCSF IL4 10 A4


title: p2dJul23.10cell 24hr MCSF IL10 10 H10

source_name: p2dJul23.10cell 24hr MCSF IL10 10 H10


title: p2dJul23.10cell 24hr MCSF IL10 10 E5

source_name: p2dJul23.10cell 24hr MCSF IL10 10 E5


title: p2dJul23.10cell 24hr MCSF IL10 10 G11

source_name: p2dJul23.10cell 24hr MCSF IL10 10 G11


title: p3dJul23.10cell 24hr MCSF CNT 10 B11

source_name: p3dJul23.10cell 24hr MCSF CNT 10 B11


title: p2dJul23.10cell 24hr MCSF IL10 10 G3

source_name: p2dJul23.10cell 24hr MCSF IL10 10 G3


title: p2dJul23.10cell 24hr MCSF IL10 10 G7

source_name: p2dJul23.10cell 24hr MCSF IL10 10 G7


title: p2dJul23.10cell 24hr MCSF IL10 10 E9

source_name: p2dJul23.10cell 24hr MCSF IL10 10 E9


title: p2dJul23.10cell 24hr MCSF IL10 10 F4

source_name: p2dJul23.10cell 24hr MCSF IL10 10 F4


title: p2dJul23.10cell 24hr MCSF IL10 10 F12

source_name: p2dJul23.10cell 24hr MCSF IL10 10 F12


title: p2dJul23.10cell 24hr MCSF IL10 10 D10

source_name: p2dJul23.10cell 24hr MCSF IL10 10 D10


title: p2dJul23.10cell 24hr MCSF IL10 10 D6

source_name: p2dJul23.10cell 24hr MCSF IL10 10 D6


title: p2dJul23.10cell 24hr MCSF IL10 10 D2

source_name: p2dJul23.10cell 24hr MCSF IL10 10 D2


title: p2dJul23.10cell 24hr MCSF IL10 10 F8

source_name: p2dJul23.10cell 24hr MCSF IL10 10 F8


title: p2dJul23.10cell 24hr MCSF IL10 10 C3

source_name: p2dJul23.10cell 24hr MCSF IL10 10 C3


title: p2dJul23.10cell 24hr MCSF IL10 10 B8

source_name: p2dJul23.10cell 24hr MCSF IL10 10 B8


title: p2dJul23.10cell 24hr MCSF IL10 10 H2

source_name: p2dJul23.10cell 24hr MCSF IL10 10 H2


title: p2dJul23.10cell 24hr MCSF IL10 10 B4

source_name: p2dJul23.10cell 24hr MCSF IL10 10 B4


title: p2dJul23.10cell 24hr MCSF IL10 10 C7

source_name: p2dJul23.10cell 24hr MCSF IL10 10 C7


title: p2dJul23.10cell 24hr MCSF IFN+TNF 10 H8

source_name: p2dJul23.10cell 24hr MCSF IFN+TNF 10 H8


title: p2dJul23.10cell 24hr MCSF IL10 10 A9

source_name: p2dJul23.10cell 24hr MCSF IL10 10 A9


title: p2dJul23.10cell 24hr MCSF IL10 10 A5

source_name: p2dJul23.10cell 24hr MCSF IL10 10 A5


title: p2dJul23.10cell 24hr MCSF IFN+TNF 10 H4

source_name: p2dJul23.10cell 24hr MCSF IFN+TNF 10 H4


title: p2dJul23.10cell 24hr MCSF IFN+TNF 10 H12


title: p2dJul23.10cell 24hr MCSF IFN+TNF 10 F10


title: p2dJul23.10cell 24hr MCSF IFN+TNF 10 G9

source_name: p2dJul23.10cell 24hr MCSF IFN+TNF 10 G9


title: p2dJul23.10cell 24hr MCSF IFN+TNF 10 F2

source_name: p2dJul23.10cell 24hr MCSF IFN+TNF 10 F2


title: p2dJul23.10cell 24hr MCSF IFN+TNF 10 G5

source_name: p2dJul23.10cell 24hr MCSF IFN+TNF 10 G5


title: p2dJul23.10cell 24hr MCSF IFN+TNF 10 F6

source_name: p2dJul23.10cell 24hr MCSF IFN+TNF 10 F6


title: p2dJul23.10cell 24hr MCSF IFN+TNF 10 E7

source_name: p2dJul23.10cell 24hr MCSF IFN+TNF 10 E7


title: p2dJul23.10cell 24hr MCSF IFN+TNF 10 E3

source_name: p2dJul23.10cell 24hr MCSF IFN+TNF 10 E3


title: p2dJul23.10cell 24hr MCSF IFN+TNF 10 D12


title: p2dJul23.10cell 24hr MCSF IFN+TNF 10 D4

source_name: p2dJul23.10cell 24hr MCSF IFN+TNF 10 D4


title: p2dJul23.10cell 24hr MCSF IFN+TNF 10 D8

source_name: p2dJul23.10cell 24hr MCSF IFN+TNF 10 D8


title: p2dJul23.10cell 24hr MCSF IL4 10 D11

source_name: p2dJul23.10cell 24hr MCSF IL4 10 D11


title: p2dJul23.10cell 24hr MCSF IFN+TNF 10 E11


title: p2dJul23.10cell 24hr MCSF IFN+TNF 10 C5

source_name: p2dJul23.10cell 24hr MCSF IFN+TNF 10 C5


title: p2dJul23.10cell 24hr MCSF IFN+TNF 10 C9

source_name: p2dJul23.10cell 24hr MCSF IFN+TNF 10 C9


title: p2dJul23.10cell 24hr MCSF IL10 10 B12

source_name: p2dJul23.10cell 24hr MCSF IL10 10 B12


title: p2dJul23.10cell 24hr MCSF IFN+TNF 10 B6

source_name: p2dJul23.10cell 24hr MCSF IFN+TNF 10 B6


title: p2dJul23.10cell 24hr MCSF IFN+TNF 10 B2

source_name: p2dJul23.10cell 24hr MCSF IFN+TNF 10 B2


title: p2dJul23.10cell 24hr MCSF IFN+TNF 10 B10


title: p2dJul23.10cell 24hr MCSF IFN+TNF 10 A7

source_name: p2dJul23.10cell 24hr MCSF IFN+TNF 10 A7


title: p2dJul23.10cell 24hr MCSF CNT 10 H5

source_name: p2dJul23.10cell 24hr MCSF CNT 10 H5


title: p2dJul23.10cell 24hr MCSF IFN+TNF 10 A11


title: p2dJul23.10cell 24hr MCSF CNT 10 H9

source_name: p2dJul23.10cell 24hr MCSF CNT 10 H9


title: p2dJul23.10cell 24hr MCSF IFN+TNF 10 A3

source_name: p2dJul23.10cell 24hr MCSF IFN+TNF 10 A3


title: p2dJul23.10cell 24hr MCSF CNT 10 G4

source_name: p2dJul23.10cell 24hr MCSF CNT 10 G4


title: p2dJul23.10cell 24hr MCSF CNT 10 G8

source_name: p2dJul23.10cell 24hr MCSF CNT 10 G8


title: p2dJul23.10cell 24hr MCSF CNT 10 F7

source_name: p2dJul23.10cell 24hr MCSF CNT 10 F7


title: p2dJul23.10cell 24hr MCSF CNT 10 F3

source_name: p2dJul23.10cell 24hr MCSF CNT 10 F3


title: p2dJul23.10cell 24hr MCSF CNT 10 G12

source_name: p2dJul23.10cell 24hr MCSF CNT 10 G12


title: p2dJul23.10cell 24hr MCSF CNT 10 F11

source_name: p2dJul23.10cell 24hr MCSF CNT 10 F11


title: p2dJul23.10cell 24hr MCSF CNT 10 E6

source_name: p2dJul23.10cell 24hr MCSF CNT 10 E6


title: p2dJul23.10cell 24hr MCSF CNT 10 E2

source_name: p2dJul23.10cell 24hr MCSF CNT 10 E2


title: p2dJul23.10cell 24hr MCSF CNT 10 E10

source_name: p2dJul23.10cell 24hr MCSF CNT 10 E10


title: p2dJul23.10cell 24hr MCSF CNT 10 D9

source_name: p2dJul23.10cell 24hr MCSF CNT 10 D9


title: p2dJul23.10cell 24hr MCSF CNT 10 C8

source_name: p2dJul23.10cell 24hr MCSF CNT 10 C8


title: p2dJul23.10cell 24hr MCSF CNT 10 C12

source_name: p2dJul23.10cell 24hr MCSF CNT 10 C12


title: p2dJul23.10cell 24hr MCSF CNT 10 C4

source_name: p2dJul23.10cell 24hr MCSF CNT 10 C4


title: p1dJul23.10cell 24hr MCSF IL4 10 H11

source_name: p1dJul23.10cell 24hr MCSF IL4 10 H11


title: p2dJul23.10cell 24hr MCSF CNT 10 B7

source_name: p2dJul23.10cell 24hr MCSF CNT 10 B7


title: p1dJul23.10cell 24hr MCSF IL4 10 G10

source_name: p1dJul23.10cell 24hr MCSF IL4 10 G10


title: p1dJul23.10cell 24hr MCSF IL4 10 G2

source_name: p1dJul23.10cell 24hr MCSF IL4 10 G2


title: p2dJul23.10cell 24hr MCSF CNT 10 B11

source_name: p2dJul23.10cell 24hr MCSF CNT 10 B11


title: p1dJul23.10cell 24hr MCSF IL4 10 H3

source_name: p1dJul23.10cell 24hr MCSF IL4 10 H3


title: p2dJul23.10cell 24hr MCSF CNT 10 A2

source_name: p2dJul23.10cell 24hr MCSF CNT 10 A2


title: p1dJul23.10cell 24hr MCSF IL4 10 G6

source_name: p1dJul23.10cell 24hr MCSF IL4 10 G6


title: p2dJul23.10cell 24hr MCSF CNT 10 B3

source_name: p2dJul23.10cell 24hr MCSF CNT 10 B3


title: p1dJul23.10cell 24hr MCSF IL4 10 F5

source_name: p1dJul23.10cell 24hr MCSF IL4 10 F5


title: p2dJul23.10cell 24hr MCSF CNT 10 A10

source_name: p2dJul23.10cell 24hr MCSF CNT 10 A10


title: p1dJul23.10cell 24hr MCSF IL4 10 H7

source_name: p1dJul23.10cell 24hr MCSF IL4 10 H7


title: p2dJul23.10cell 24hr MCSF CNT 10 A6

source_name: p2dJul23.10cell 24hr MCSF CNT 10 A6


title: p1dJul23.10cell 24hr MCSF IL4 10 C6

source_name: p1dJul23.10cell 24hr MCSF IL4 10 C6


title: p2dJul23.10cell 24hr MCSF CNT 10 D5

source_name: p2dJul23.10cell 24hr MCSF CNT 10 D5


title: p1dJul23.10cell 24hr MCSF IL4 10 D7

source_name: p1dJul23.10cell 24hr MCSF IL4 10 D7


title: p1dJul23.10cell 24hr MCSF IL4 10 D3

source_name: p1dJul23.10cell 24hr MCSF IL4 10 D3


title: p1dJul23.10cell 24hr MCSF IL4 10 D11

source_name: p1dJul23.10cell 24hr MCSF IL4 10 D11


title: p1dJul23.10cell 24hr MCSF IL4 10 E12

source_name: p1dJul23.10cell 24hr MCSF IL4 10 E12


title: p1dJul23.10cell 24hr MCSF IL4 10 E4

source_name: p1dJul23.10cell 24hr MCSF IL4 10 E4


title: p1dJul23.10cell 24hr MCSF IL4 10 E8

source_name: p1dJul23.10cell 24hr MCSF IL4 10 E8


title: p1dJul23.10cell 24hr MCSF IL4 10 F9

source_name: p1dJul23.10cell 24hr MCSF IL4 10 F9


title: p1dJul23.10cell 24hr MCSF IL4 10 C2

source_name: p1dJul23.10cell 24hr MCSF IL4 10 C2


title: p1dJul23.10cell 24hr MCSF IL4 10 C10

source_name: p1dJul23.10cell 24hr MCSF IL4 10 C10


title: p1dJul23.10cell 24hr MCSF IL4 10 B5

source_name: p1dJul23.10cell 24hr MCSF IL4 10 B5


title: p1dJul23.10cell 24hr MCSF IL10 10 H6

source_name: p1dJul23.10cell 24hr MCSF IL10 10 H6


title: p1dJul23.10cell 24hr MCSF IL4 10 A4

source_name: p1dJul23.10cell 24hr MCSF IL4 10 A4


title: p1dJul23.10cell 24hr MCSF IL4 10 A8

source_name: p1dJul23.10cell 24hr MCSF IL4 10 A8


title: p1dJul23.10cell 24hr MCSF IL4 10 A12

source_name: p1dJul23.10cell 24hr MCSF IL4 10 A12


title: p1dJul23.10cell 24hr MCSF IL10 10 H2

source_name: p1dJul23.10cell 24hr MCSF IL10 10 H2


title: p1dJul23.10cell 24hr MCSF IL10 10 H10

source_name: p1dJul23.10cell 24hr MCSF IL10 10 H10


title: p1dJul23.10cell 24hr MCSF IL10 10 F8

source_name: p1dJul23.10cell 24hr MCSF IL10 10 F8


title: p1dJul23.10cell 24hr MCSF IL4 10 B9

source_name: p1dJul23.10cell 24hr MCSF IL4 10 B9


title: p1dJul23.10cell 24hr MCSF IL10 10 G7

source_name: p1dJul23.10cell 24hr MCSF IL10 10 G7


title: p1dJul23.10cell 24hr MCSF IL10 10 G3

source_name: p1dJul23.10cell 24hr MCSF IL10 10 G3


title: p1dJul23.10cell 24hr MCSF IL10 10 G11

source_name: p1dJul23.10cell 24hr MCSF IL10 10 G11


title: p1dJul23.10cell 24hr MCSF IL10 10 E9

source_name: p1dJul23.10cell 24hr MCSF IL10 10 E9


title: p1dJul23.10cell 24hr MCSF IL10 10 F4

source_name: p1dJul23.10cell 24hr MCSF IL10 10 F4


title: p1dJul23.10cell 24hr MCSF IL10 10 F12

source_name: p1dJul23.10cell 24hr MCSF IL10 10 F12


title: p1dJul23.10cell 24hr MCSF IL10 10 D2

source_name: p1dJul23.10cell 24hr MCSF IL10 10 D2


title: p1dJul23.10cell 24hr MCSF IL10 10 E5

source_name: p1dJul23.10cell 24hr MCSF IL10 10 E5


title: p1dJul23.10cell 24hr MCSF IFN+TNF 10 H8

source_name: p1dJul23.10cell 24hr MCSF IFN+TNF 10 H8


title: p1dJul23.10cell 24hr MCSF IL10 10 B4

source_name: p1dJul23.10cell 24hr MCSF IL10 10 B4


title: p1dJul23.10cell 24hr MCSF IL10 10 B8

source_name: p1dJul23.10cell 24hr MCSF IL10 10 B8


title: p1dJul23.10cell 24hr MCSF IL10 10 C11

source_name: p1dJul23.10cell 24hr MCSF IL10 10 C11


title: p1dJul23.10cell 24hr MCSF IL10 10 D6

source_name: p1dJul23.10cell 24hr MCSF IL10 10 D6


title: p1dJul23.10cell 24hr MCSF IL10 10 C3

source_name: p1dJul23.10cell 24hr MCSF IL10 10 C3


title: p1dJul23.10cell 24hr MCSF IL10 10 A9

source_name: p1dJul23.10cell 24hr MCSF IL10 10 A9


title: p1dJul23.10cell 24hr MCSF IL10 10 A5

source_name: p1dJul23.10cell 24hr MCSF IL10 10 A5


title: p1dJul23.10cell 24hr MCSF IFN+TNF 10 H12


title: p1dJul23.10cell 24hr MCSF IFN+TNF 10 H4

source_name: p1dJul23.10cell 24hr MCSF IFN+TNF 10 H4


title: p1dJul23.10cell 24hr MCSF IFN+TNF 10 G9

source_name: p1dJul23.10cell 24hr MCSF IFN+TNF 10 G9


title: p1dJul23.10cell 24hr MCSF IL10 10 C7

source_name: p1dJul23.10cell 24hr MCSF IL10 10 C7


title: p1dJul23.10cell 24hr MCSF IL10 10 D10

source_name: p1dJul23.10cell 24hr MCSF IL10 10 D10


title: p1dJul23.10cell 24hr MCSF IL10 10 B12

source_name: p1dJul23.10cell 24hr MCSF IL10 10 B12


title: p1dJul23.10cell 24hr MCSF IFN+TNF 10 F6

source_name: p1dJul23.10cell 24hr MCSF IFN+TNF 10 F6


title: p1dJul23.10cell 24hr MCSF IFN+TNF 10 F2

source_name: p1dJul23.10cell 24hr MCSF IFN+TNF 10 F2


title: p1dJul23.10cell 24hr MCSF IFN+TNF 10 G5

source_name: p1dJul23.10cell 24hr MCSF IFN+TNF 10 G5


title: p1dJul23.10cell 24hr MCSF IFN+TNF 10 E7

source_name: p1dJul23.10cell 24hr MCSF IFN+TNF 10 E7


title: p1dJul23.10cell 24hr MCSF IFN+TNF 10 F10


title: p1dJul23.10cell 24hr MCSF IFN+TNF 10 D4

source_name: p1dJul23.10cell 24hr MCSF IFN+TNF 10 D4


title: p1dJul23.10cell 24hr MCSF IFN+TNF 10 C9

source_name: p1dJul23.10cell 24hr MCSF IFN+TNF 10 C9


title: p1dJul23.10cell 24hr MCSF IFN+TNF 10 B2

source_name: p1dJul23.10cell 24hr MCSF IFN+TNF 10 B2


title: p1dJul23.10cell 24hr MCSF IFN+TNF 10 D8

source_name: p1dJul23.10cell 24hr MCSF IFN+TNF 10 D8


title: p1dJul23.10cell 24hr MCSF IFN+TNF 10 E11


title: p1dJul23.10cell 24hr MCSF IFN+TNF 10 C5

source_name: p1dJul23.10cell 24hr MCSF IFN+TNF 10 C5


title: p1dJul23.10cell 24hr MCSF CNT 10 H5

source_name: p1dJul23.10cell 24hr MCSF CNT 10 H5


title: p1dJul23.10cell 24hr MCSF CNT 10 G8

source_name: p1dJul23.10cell 24hr MCSF CNT 10 G8


title: p1dJul23.10cell 24hr MCSF IFN+TNF 10 A11


title: p1dJul23.10cell 24hr MCSF CNT 10 G4

source_name: p1dJul23.10cell 24hr MCSF CNT 10 G4


title: p1dJul23.10cell 24hr MCSF CNT 10 G12

source_name: p1dJul23.10cell 24hr MCSF CNT 10 G12


title: p1dJul23.10cell 24hr MCSF CNT 10 F7

source_name: p1dJul23.10cell 24hr MCSF CNT 10 F7


title: p1dJul23.10cell 24hr MCSF IFN+TNF 10 D12


title: p1dJul23.10cell 24hr MCSF IFN+TNF 10 A3

source_name: p1dJul23.10cell 24hr MCSF IFN+TNF 10 A3


title: p1dJul23.10cell 24hr MCSF CNT 10 E2

source_name: p1dJul23.10cell 24hr MCSF CNT 10 E2


title: p1dJul23.10cell 24hr MCSF IFN+TNF 10 B10


title: p1dJul23.10cell 24hr MCSF CNT 10 E10

source_name: p1dJul23.10cell 24hr MCSF CNT 10 E10


title: p1dJul23.10cell 24hr MCSF CNT 10 D9

source_name: p1dJul23.10cell 24hr MCSF CNT 10 D9


title: p1dJul23.10cell 24hr MCSF IFN+TNF 10 A7

source_name: p1dJul23.10cell 24hr MCSF IFN+TNF 10 A7


title: p1dJul23.10cell 24hr MCSF CNT 10 E6

source_name: p1dJul23.10cell 24hr MCSF CNT 10 E6


title: p1dJul23.10cell 24hr MCSF CNT 10 F11

source_name: p1dJul23.10cell 24hr MCSF CNT 10 F11


title: p1dJul23.10cell 24hr MCSF CNT 10 H9

source_name: p1dJul23.10cell 24hr MCSF CNT 10 H9


title: p1dJul23.10cell 24hr MCSF CNT 10 F3

source_name: p1dJul23.10cell 24hr MCSF CNT 10 F3


title: p1dJul23.10cell 24hr MCSF CNT 10 C8

source_name: p1dJul23.10cell 24hr MCSF CNT 10 C8


title: p1dJul23.10cell 24hr MCSF CNT 10 C12

source_name: p1dJul23.10cell 24hr MCSF CNT 10 C12


title: p1dJul23.10cell 24hr MCSF IFN+TNF 10 B6

source_name: p1dJul23.10cell 24hr MCSF IFN+TNF 10 B6


title: p1dJul23.10cell 24hr MCSF CNT 10 B7

source_name: p1dJul23.10cell 24hr MCSF CNT 10 B7


title: p1dJul23.10cell 24hr MCSF CNT 10 C4

source_name: p1dJul23.10cell 24hr MCSF CNT 10 C4


title: p1dJul23.10cell 24hr MCSF CNT 10 A2

source_name: p1dJul23.10cell 24hr MCSF CNT 10 A2


title: p1dJul23.10cell 24hr MCSF CNT 10 A6

source_name: p1dJul23.10cell 24hr MCSF CNT 10 A6


title: p1dJul23.10cell 24hr MCSF CNT 10 B11

source_name: p1dJul23.10cell 24hr MCSF CNT 10 B11


title: p1dJul23.10cell 24hr MCSF CNT 10 B3

source_name: p1dJul23.10cell 24hr MCSF CNT 10 B3


title: p1dJul23.10cell 24hr MCSF CNT 10 A10

source_name: p1dJul23.10cell 24hr MCSF CNT 10 A10


title: p1dJul23.10cell 24hr MCSF IFN+TNF 10 E3

source_name: p1dJul23.10cell 24hr MCSF IFN+TNF 10 E3


title: p1dJul23.10cell 24hr MCSF CNT 10 D5

source_name: p1dJul23.10cell 24hr MCSF CNT 10 D5


title: p4dJul9.10cell 24hr MCSF IL4 10 F9

source_name: p4dJul9.10cell 24hr MCSF IL4 10 F9


title: p4dJul9.10cell 24hr MCSF IL4 10 H11

source_name: p4dJul9.10cell 24hr MCSF IL4 10 H11


title: p4dJul9.10cell 24hr MCSF IL4 10 G10

source_name: p4dJul9.10cell 24hr MCSF IL4 10 G10


title: p4dJul9.10cell 24hr MCSF IL4 10 F5

source_name: p4dJul9.10cell 24hr MCSF IL4 10 F5


title: p4dJul9.10cell 24hr MCSF IL4 10 H3

source_name: p4dJul9.10cell 24hr MCSF IL4 10 H3


title: p4dJul9.10cell 24hr MCSF IL4 10 H7

source_name: p4dJul9.10cell 24hr MCSF IL4 10 H7


title: p4dJul9.10cell 24hr MCSF IL4 10 A4

source_name: p4dJul9.10cell 24hr MCSF IL4 10 A4


title: p4dJul9.10cell 24hr MCSF IL4 10 A8

source_name: p4dJul9.10cell 24hr MCSF IL4 10 A8


title: p4dJul9.10cell 24hr MCSF IL4 10 B5

source_name: p4dJul9.10cell 24hr MCSF IL4 10 B5


title: p4dJul9.10cell 24hr MCSF IL4 10 C6

source_name: p4dJul9.10cell 24hr MCSF IL4 10 C6


title: p4dJul9.10cell 24hr MCSF IL4 10 E4

source_name: p4dJul9.10cell 24hr MCSF IL4 10 E4


title: p4dJul9.10cell 24hr MCSF IL4 10 E8

source_name: p4dJul9.10cell 24hr MCSF IL4 10 E8


title: p4dJul9.10cell 24hr MCSF IL4 10 C2

source_name: p4dJul9.10cell 24hr MCSF IL4 10 C2


title: p4dJul9.10cell 24hr MCSF IL4 10 E12

source_name: p4dJul9.10cell 24hr MCSF IL4 10 E12


title: p4dJul9.10cell 24hr MCSF IL4 10 D11

source_name: p4dJul9.10cell 24hr MCSF IL4 10 D11


title: p4dJul9.10cell 24hr MCSF IL4 10 D3

source_name: p4dJul9.10cell 24hr MCSF IL4 10 D3


title: p4dJul9.10cell 24hr MCSF IL4 10 D7

source_name: p4dJul9.10cell 24hr MCSF IL4 10 D7


title: p4dJul9.10cell 24hr MCSF IL4 10 B9

source_name: p4dJul9.10cell 24hr MCSF IL4 10 B9


title: p4dJul9.10cell 24hr MCSF IL4 10 G2

source_name: p4dJul9.10cell 24hr MCSF IL4 10 G2


title: p4dJul9.10cell 24hr MCSF IL4 10 G6

source_name: p4dJul9.10cell 24hr MCSF IL4 10 G6


title: p4dJul9.10cell 24hr MCSF IL10 10 H10

source_name: p4dJul9.10cell 24hr MCSF IL10 10 H10


title: p4dJul9.10cell 24hr MCSF IL4 10 A12

source_name: p4dJul9.10cell 24hr MCSF IL4 10 A12


title: p4dJul9.10cell 24hr MCSF IL10 10 H2

source_name: p4dJul9.10cell 24hr MCSF IL10 10 H2


title: p4dJul9.10cell 24hr MCSF IL10 10 H6

source_name: p4dJul9.10cell 24hr MCSF IL10 10 H6


title: p4dJul9.10cell 24hr MCSF IL10 10 G11

source_name: p4dJul9.10cell 24hr MCSF IL10 10 G11


title: p4dJul9.10cell 24hr MCSF IL10 10 G7

source_name: p4dJul9.10cell 24hr MCSF IL10 10 G7


title: p4dJul9.10cell 24hr MCSF IL10 10 G3

source_name: p4dJul9.10cell 24hr MCSF IL10 10 G3


title: p4dJul9.10cell 24hr MCSF IL10 10 F8

source_name: p4dJul9.10cell 24hr MCSF IL10 10 F8


title: p4dJul9.10cell 24hr MCSF IL4 10 C10

source_name: p4dJul9.10cell 24hr MCSF IL4 10 C10


title: p4dJul9.10cell 24hr MCSF IL10 10 F4

source_name: p4dJul9.10cell 24hr MCSF IL10 10 F4


title: p4dJul9.10cell 24hr MCSF IL10 10 F12

source_name: p4dJul9.10cell 24hr MCSF IL10 10 F12


title: p4dJul9.10cell 24hr MCSF IL10 10 D10

source_name: p4dJul9.10cell 24hr MCSF IL10 10 D10


title: p4dJul9.10cell 24hr MCSF IL10 10 D6

source_name: p4dJul9.10cell 24hr MCSF IL10 10 D6


title: p4dJul9.10cell 24hr MCSF IL10 10 D2

source_name: p4dJul9.10cell 24hr MCSF IL10 10 D2


title: p4dJul9.10cell 24hr MCSF IL10 10 C7

source_name: p4dJul9.10cell 24hr MCSF IL10 10 C7


title: p4dJul9.10cell 24hr MCSF IL10 10 C3

source_name: p4dJul9.10cell 24hr MCSF IL10 10 C3


title: p4dJul9.10cell 24hr MCSF IL10 10 C11

source_name: p4dJul9.10cell 24hr MCSF IL10 10 C11


title: p4dJul9.10cell 24hr MCSF IL10 10 A9

source_name: p4dJul9.10cell 24hr MCSF IL10 10 A9


title: p4dJul9.10cell 24hr MCSF IL10 10 E9

source_name: p4dJul9.10cell 24hr MCSF IL10 10 E9


title: p4dJul9.10cell 24hr MCSF IL10 10 E5

source_name: p4dJul9.10cell 24hr MCSF IL10 10 E5


title: p4dJul9.10cell 24hr MCSF IL10 10 A5

source_name: p4dJul9.10cell 24hr MCSF IL10 10 A5


title: p4dJul9.10cell 24hr MCSF IL10 10 B12

source_name: p4dJul9.10cell 24hr MCSF IL10 10 B12


title: p3dJul9.10cell 24hr MCSF IL4 10 G2

source_name: p3dJul9.10cell 24hr MCSF IL4 10 G2


title: p3dJul9.10cell 24hr MCSF IL4 10 H3

source_name: p3dJul9.10cell 24hr MCSF IL4 10 H3


title: p4dJul9.10cell 24hr MCSF IL10 10 B4

source_name: p4dJul9.10cell 24hr MCSF IL10 10 B4


title: p4dJul9.10cell 24hr MCSF IL10 10 B8

source_name: p4dJul9.10cell 24hr MCSF IL10 10 B8


title: p3dJul9.10cell 24hr MCSF IL4 10 H11

source_name: p3dJul9.10cell 24hr MCSF IL4 10 H11


title: p3dJul9.10cell 24hr MCSF IL4 10 G6

source_name: p3dJul9.10cell 24hr MCSF IL4 10 G6


title: p3dJul9.10cell 24hr MCSF IL4 10 E8

source_name: p3dJul9.10cell 24hr MCSF IL4 10 E8


title: p3dJul9.10cell 24hr MCSF IL4 10 H7

source_name: p3dJul9.10cell 24hr MCSF IL4 10 H7


title: p3dJul9.10cell 24hr MCSF IL4 10 G10

source_name: p3dJul9.10cell 24hr MCSF IL4 10 G10


title: p3dJul9.10cell 24hr MCSF IL4 10 F9

source_name: p3dJul9.10cell 24hr MCSF IL4 10 F9


title: p3dJul9.10cell 24hr MCSF IL4 10 F5

source_name: p3dJul9.10cell 24hr MCSF IL4 10 F5


title: p3dJul9.10cell 24hr MCSF IL4 10 E12

source_name: p3dJul9.10cell 24hr MCSF IL4 10 E12


title: p3dJul9.10cell 24hr MCSF IL4 10 D7

source_name: p3dJul9.10cell 24hr MCSF IL4 10 D7


title: p3dJul9.10cell 24hr MCSF IL4 10 E4

source_name: p3dJul9.10cell 24hr MCSF IL4 10 E4


title: p3dJul9.10cell 24hr MCSF IL4 10 D3

source_name: p3dJul9.10cell 24hr MCSF IL4 10 D3


title: p3dJul9.10cell 24hr MCSF IL4 10 D11

source_name: p3dJul9.10cell 24hr MCSF IL4 10 D11


title: p3dJul9.10cell 24hr MCSF IL4 10 C6

source_name: p3dJul9.10cell 24hr MCSF IL4 10 C6


title: p3dJul9.10cell 24hr MCSF IL4 10 A8

source_name: p3dJul9.10cell 24hr MCSF IL4 10 A8


title: p3dJul9.10cell 24hr MCSF IL4 10 B5

source_name: p3dJul9.10cell 24hr MCSF IL4 10 B5


title: p3dJul9.10cell 24hr MCSF IL4 10 C2

source_name: p3dJul9.10cell 24hr MCSF IL4 10 C2


title: p3dJul9.10cell 24hr MCSF IL4 10 B9

source_name: p3dJul9.10cell 24hr MCSF IL4 10 B9


title: p3dJul9.10cell 24hr MCSF IL4 10 C10

source_name: p3dJul9.10cell 24hr MCSF IL4 10 C10


title: p3dJul9.10cell 24hr MCSF IL4 10 A4

source_name: p3dJul9.10cell 24hr MCSF IL4 10 A4


title: p3dJul9.10cell 24hr MCSF IL4 10 A12

source_name: p3dJul9.10cell 24hr MCSF IL4 10 A12


title: p3dJul9.10cell 24hr MCSF IL10 10 G11

source_name: p3dJul9.10cell 24hr MCSF IL10 10 G11


title: p3dJul9.10cell 24hr MCSF IL10 10 G7

source_name: p3dJul9.10cell 24hr MCSF IL10 10 G7


title: p3dJul9.10cell 24hr MCSF IL10 10 F8

source_name: p3dJul9.10cell 24hr MCSF IL10 10 F8


title: p3dJul9.10cell 24hr MCSF IL10 10 G3

source_name: p3dJul9.10cell 24hr MCSF IL10 10 G3


title: p3dJul9.10cell 24hr MCSF IL10 10 H10

source_name: p3dJul9.10cell 24hr MCSF IL10 10 H10


title: p3dJul9.10cell 24hr MCSF IL10 10 E5

source_name: p3dJul9.10cell 24hr MCSF IL10 10 E5


title: p3dJul9.10cell 24hr MCSF IL10 10 F4

source_name: p3dJul9.10cell 24hr MCSF IL10 10 F4


title: p3dJul9.10cell 24hr MCSF IL10 10 D6

source_name: p3dJul9.10cell 24hr MCSF IL10 10 D6


title: p3dJul9.10cell 24hr MCSF IL10 10 C3

source_name: p3dJul9.10cell 24hr MCSF IL10 10 C3


title: p3dJul9.10cell 24hr MCSF IL10 10 H6

source_name: p3dJul9.10cell 24hr MCSF IL10 10 H6


title: p3dJul9.10cell 24hr MCSF IL10 10 H2

source_name: p3dJul9.10cell 24hr MCSF IL10 10 H2


title: p3dJul9.10cell 24hr MCSF IL10 10 D10

source_name: p3dJul9.10cell 24hr MCSF IL10 10 D10


title: p3dJul9.10cell 24hr MCSF IL10 10 C7

source_name: p3dJul9.10cell 24hr MCSF IL10 10 C7


title: p3dJul9.10cell 24hr MCSF IL10 10 B8

source_name: p3dJul9.10cell 24hr MCSF IL10 10 B8


title: p3dJul9.10cell 24hr MCSF IL10 10 F12

source_name: p3dJul9.10cell 24hr MCSF IL10 10 F12


title: p3dJul9.10cell 24hr MCSF IL10 10 D2

source_name: p3dJul9.10cell 24hr MCSF IL10 10 D2


title: p3dJul9.10cell 24hr MCSF IL10 10 B4

source_name: p3dJul9.10cell 24hr MCSF IL10 10 B4


title: p3dJul9.10cell 24hr MCSF IL10 10 B12

source_name: p3dJul9.10cell 24hr MCSF IL10 10 B12


title: p3dJul9.10cell 24hr MCSF IL10 10 E9

source_name: p3dJul9.10cell 24hr MCSF IL10 10 E9


title: p3dJul9.10cell 24hr MCSF IL10 10 C11

source_name: p3dJul9.10cell 24hr MCSF IL10 10 C11


title: p3dJul9.10cell 24hr MCSF IL10 10 A9

source_name: p3dJul9.10cell 24hr MCSF IL10 10 A9


title: p3dJul9.10cell 24hr MCSF IL10 10 A5

source_name: p3dJul9.10cell 24hr MCSF IL10 10 A5


title: p2dJul9.10cell 24hr MCSF IL4 10 H3

source_name: p2dJul9.10cell 24hr MCSF IL4 10 H3


title: p2dJul9.10cell 24hr MCSF IL4 10 G6

source_name: p2dJul9.10cell 24hr MCSF IL4 10 G6


title: p2dJul9.10cell 24hr MCSF IL4 10 H7

source_name: p2dJul9.10cell 24hr MCSF IL4 10 H7


title: p2dJul9.10cell 24hr MCSF IL4 10 H11

source_name: p2dJul9.10cell 24hr MCSF IL4 10 H11


title: p2dJul9.10cell 24hr MCSF IL4 10 G2

source_name: p2dJul9.10cell 24hr MCSF IL4 10 G2


title: p2dJul9.10cell 24hr MCSF IL4 10 F9

source_name: p2dJul9.10cell 24hr MCSF IL4 10 F9


title: p2dJul9.10cell 24hr MCSF IL4 10 G10

source_name: p2dJul9.10cell 24hr MCSF IL4 10 G10


title: p2dJul9.10cell 24hr MCSF IL4 10 F5

source_name: p2dJul9.10cell 24hr MCSF IL4 10 F5


title: p2dJul9.10cell 24hr MCSF IL4 10 D3

source_name: p2dJul9.10cell 24hr MCSF IL4 10 D3


title: p2dJul9.10cell 24hr MCSF IL4 10 E12

source_name: p2dJul9.10cell 24hr MCSF IL4 10 E12


title: p2dJul9.10cell 24hr MCSF IL4 10 E4

source_name: p2dJul9.10cell 24hr MCSF IL4 10 E4


title: p2dJul9.10cell 24hr MCSF IL4 10 E8

source_name: p2dJul9.10cell 24hr MCSF IL4 10 E8


title: p2dJul9.10cell 24hr MCSF IL4 10 D11

source_name: p2dJul9.10cell 24hr MCSF IL4 10 D11


title: p2dJul9.10cell 24hr MCSF IL4 10 C2

source_name: p2dJul9.10cell 24hr MCSF IL4 10 C2


title: p2dJul9.10cell 24hr MCSF IL4 10 C6

source_name: p2dJul9.10cell 24hr MCSF IL4 10 C6


title: p2dJul9.10cell 24hr MCSF IL4 10 B9

source_name: p2dJul9.10cell 24hr MCSF IL4 10 B9


title: p2dJul9.10cell 24hr MCSF IL4 10 C10

source_name: p2dJul9.10cell 24hr MCSF IL4 10 C10


title: p2dJul9.10cell 24hr MCSF IL4 10 A8

source_name: p2dJul9.10cell 24hr MCSF IL4 10 A8


title: p2dJul9.10cell 24hr MCSF IL4 10 A12

source_name: p2dJul9.10cell 24hr MCSF IL4 10 A12


title: p2dJul9.10cell 24hr MCSF IL10 10 H6

source_name: p2dJul9.10cell 24hr MCSF IL10 10 H6


title: p2dJul9.10cell 24hr MCSF IL4 10 D7

source_name: p2dJul9.10cell 24hr MCSF IL4 10 D7


title: p2dJul9.10cell 24hr MCSF IL10 10 G3

source_name: p2dJul9.10cell 24hr MCSF IL10 10 G3


title: p2dJul9.10cell 24hr MCSF IL10 10 H2

source_name: p2dJul9.10cell 24hr MCSF IL10 10 H2


title: p2dJul9.10cell 24hr MCSF IL10 10 G7

source_name: p2dJul9.10cell 24hr MCSF IL10 10 G7


title: p2dJul9.10cell 24hr MCSF IL4 10 B5

source_name: p2dJul9.10cell 24hr MCSF IL4 10 B5


title: p2dJul9.10cell 24hr MCSF IL4 10 A4

source_name: p2dJul9.10cell 24hr MCSF IL4 10 A4


title: p2dJul9.10cell 24hr MCSF IL10 10 H10

source_name: p2dJul9.10cell 24hr MCSF IL10 10 H10


title: p2dJul9.10cell 24hr MCSF IL10 10 F4

source_name: p2dJul9.10cell 24hr MCSF IL10 10 F4


title: p2dJul9.10cell 24hr MCSF IL10 10 G11

source_name: p2dJul9.10cell 24hr MCSF IL10 10 G11


title: p2dJul9.10cell 24hr MCSF IL10 10 F8

source_name: p2dJul9.10cell 24hr MCSF IL10 10 F8


title: p2dJul9.10cell 24hr MCSF IL10 10 C11

source_name: p2dJul9.10cell 24hr MCSF IL10 10 C11


title: p2dJul9.10cell 24hr MCSF IL10 10 D2

source_name: p2dJul9.10cell 24hr MCSF IL10 10 D2


title: p2dJul9.10cell 24hr MCSF IL10 10 D6

source_name: p2dJul9.10cell 24hr MCSF IL10 10 D6


title: p2dJul9.10cell 24hr MCSF IL10 10 E5

source_name: p2dJul9.10cell 24hr MCSF IL10 10 E5


title: p2dJul9.10cell 24hr MCSF IL10 10 E9

source_name: p2dJul9.10cell 24hr MCSF IL10 10 E9


title: p2dJul9.10cell 24hr MCSF IL10 10 A5

source_name: p2dJul9.10cell 24hr MCSF IL10 10 A5


title: p2dJul9.10cell 24hr MCSF IL10 10 D10

source_name: p2dJul9.10cell 24hr MCSF IL10 10 D10


title: p2dJul9.10cell 24hr MCSF IL10 10 F12

source_name: p2dJul9.10cell 24hr MCSF IL10 10 F12


title: p2dJul9.10cell 24hr MCSF IL10 10 C7

source_name: p2dJul9.10cell 24hr MCSF IL10 10 C7


title: p2dJul9.10cell 24hr MCSF IL10 10 B8

source_name: p2dJul9.10cell 24hr MCSF IL10 10 B8


title: p1dJul9.10cell 24hr MCSF IL4 10 G2

source_name: p1dJul9.10cell 24hr MCSF IL4 10 G2


title: p1dJul9.10cell 24hr MCSF IL4 10 F5

source_name: p1dJul9.10cell 24hr MCSF IL4 10 F5


title: p1dJul9.10cell 24hr MCSF IL4 10 G10

source_name: p1dJul9.10cell 24hr MCSF IL4 10 G10


title: p2dJul9.10cell 24hr MCSF IL10 10 A9

source_name: p2dJul9.10cell 24hr MCSF IL10 10 A9


title: p2dJul9.10cell 24hr MCSF IL10 10 B12

source_name: p2dJul9.10cell 24hr MCSF IL10 10 B12


title: p1dJul9.10cell 24hr MCSF IL4 10 E12

source_name: p1dJul9.10cell 24hr MCSF IL4 10 E12


title: p1dJul9.10cell 24hr MCSF IL4 10 E8

source_name: p1dJul9.10cell 24hr MCSF IL4 10 E8


title: p1dJul9.10cell 24hr MCSF IL4 10 G6

source_name: p1dJul9.10cell 24hr MCSF IL4 10 G6


title: p2dJul9.10cell 24hr MCSF IL10 10 B4

source_name: p2dJul9.10cell 24hr MCSF IL10 10 B4


title: p1dJul9.10cell 24hr MCSF IL4 10 H7

source_name: p1dJul9.10cell 24hr MCSF IL4 10 H7


title: p2dJul9.10cell 24hr MCSF IL10 10 C3

source_name: p2dJul9.10cell 24hr MCSF IL10 10 C3


title: p1dJul9.10cell 24hr MCSF IL4 10 E4

source_name: p1dJul9.10cell 24hr MCSF IL4 10 E4


title: p1dJul9.10cell 24hr MCSF IL4 10 H11

source_name: p1dJul9.10cell 24hr MCSF IL4 10 H11


title: p1dJul9.10cell 24hr MCSF IL4 10 F9

source_name: p1dJul9.10cell 24hr MCSF IL4 10 F9


title: p1dJul9.10cell 24hr MCSF IL4 10 H3

source_name: p1dJul9.10cell 24hr MCSF IL4 10 H3


title: p1dJul9.10cell 24hr MCSF IL4 10 C6

source_name: p1dJul9.10cell 24hr MCSF IL4 10 C6


title: p1dJul9.10cell 24hr MCSF IL4 10 C2

source_name: p1dJul9.10cell 24hr MCSF IL4 10 C2


title: p1dJul9.10cell 24hr MCSF IL4 10 D3

source_name: p1dJul9.10cell 24hr MCSF IL4 10 D3


title: p1dJul9.10cell 24hr MCSF IL4 10 D7

source_name: p1dJul9.10cell 24hr MCSF IL4 10 D7


title: p1dJul9.10cell 24hr MCSF IL4 10 B9

source_name: p1dJul9.10cell 24hr MCSF IL4 10 B9


title: p1dJul9.10cell 24hr MCSF IL4 10 C10

source_name: p1dJul9.10cell 24hr MCSF IL4 10 C10


title: p1dJul9.10cell 24hr MCSF IL4 10 B5

source_name: p1dJul9.10cell 24hr MCSF IL4 10 B5


title: p1dJul9.10cell 24hr MCSF IL4 10 D11

source_name: p1dJul9.10cell 24hr MCSF IL4 10 D11


title: p1dJul9.10cell 24hr MCSF IL10 10 G11

source_name: p1dJul9.10cell 24hr MCSF IL10 10 G11


title: p1dJul9.10cell 24hr MCSF IL10 10 H10

source_name: p1dJul9.10cell 24hr MCSF IL10 10 H10


title: p1dJul9.10cell 24hr MCSF IL4 10 A12

source_name: p1dJul9.10cell 24hr MCSF IL4 10 A12


title: p1dJul9.10cell 24hr MCSF IL4 10 A8

source_name: p1dJul9.10cell 24hr MCSF IL4 10 A8


title: p1dJul9.10cell 24hr MCSF IL4 10 A4

source_name: p1dJul9.10cell 24hr MCSF IL4 10 A4


title: p1dJul9.10cell 24hr MCSF IL10 10 G7

source_name: p1dJul9.10cell 24hr MCSF IL10 10 G7


title: p1dJul9.10cell 24hr MCSF IL10 10 G3

source_name: p1dJul9.10cell 24hr MCSF IL10 10 G3


title: p1dJul9.10cell 24hr MCSF IL10 10 H2

source_name: p1dJul9.10cell 24hr MCSF IL10 10 H2


title: p1dJul9.10cell 24hr MCSF IL10 10 D10

source_name: p1dJul9.10cell 24hr MCSF IL10 10 D10


title: p1dJul9.10cell 24hr MCSF IL10 10 D6

source_name: p1dJul9.10cell 24hr MCSF IL10 10 D6


title: p1dJul9.10cell 24hr MCSF IL10 10 H6

source_name: p1dJul9.10cell 24hr MCSF IL10 10 H6


title: p1dJul9.10cell 24hr MCSF IL10 10 E5

source_name: p1dJul9.10cell 24hr MCSF IL10 10 E5


title: p1dJul9.10cell 24hr MCSF IL10 10 C7

source_name: p1dJul9.10cell 24hr MCSF IL10 10 C7


title: p1dJul9.10cell 24hr MCSF IL10 10 F8

source_name: p1dJul9.10cell 24hr MCSF IL10 10 F8


title: p1dJul9.10cell 24hr MCSF IL10 10 C3

source_name: p1dJul9.10cell 24hr MCSF IL10 10 C3


title: p1dJul9.10cell 24hr MCSF IL10 10 D2

source_name: p1dJul9.10cell 24hr MCSF IL10 10 D2


title: p1dJul9.10cell 24hr MCSF IL10 10 F12

source_name: p1dJul9.10cell 24hr MCSF IL10 10 F12


title: p1dJul9.10cell 24hr MCSF IL10 10 E9

source_name: p1dJul9.10cell 24hr MCSF IL10 10 E9


title: p1dJul9.10cell 24hr MCSF IL10 10 C11

source_name: p1dJul9.10cell 24hr MCSF IL10 10 C11


title: p1dJul9.10cell 24hr MCSF IL10 10 A5

source_name: p1dJul9.10cell 24hr MCSF IL10 10 A5


title: p1dJul9.10cell 24hr MCSF IL10 10 F4

source_name: p1dJul9.10cell 24hr MCSF IL10 10 F4


title: p1dJul9.10cell 24hr MCSF IL10 10 B12

source_name: p1dJul9.10cell 24hr MCSF IL10 10 B12


title: p1dJul9.10cell 24hr MCSF IL10 10 A9

source_name: p1dJul9.10cell 24hr MCSF IL10 10 A9


title: p5AdJul9 24hr MCSF IL4 E12

description: Single human monocyte-dervide macrophage assayed by fluidigm qPCR

data_processing: We convert gene expression data exported from Fluidigm Real-time PCR Analysis software from Ct (Cycle threshold) units to the more convenient Et=40-Ct units. To correct for plate-plate effects, we shifted all detected gene measurements in a plate by a plate-specific global factor, which is chosen such that the median ERCC-0003 level of the shifted data across all samples in the plate becomes the same across all plates.

source_name: Human monocyte derived macrophages

label_protocol: Sort plates containing single cell RNA were thawed, and 1.35 uL of STA mix (1.25 uL of a pool of 96 Taqman assays at 0.2x concentration, and 0.1 uL Superscript III/Platinum Taq mix (CellsDirect kit, Life Technologies)) were added to each well. Plates were then sealed with clear film (Bio-rad) and centrifuged at 2000x G for 3 minutes and STA was run on a CFX Connect thermal cycler (Bio-rad) with the following cycling parameters: 50°C for 15 mins, 95°C for 2 minutes, then 18 cycles of 95°C for 15 seconds and 60°C for 4 minutes. Plates were then placed on ice and cold 10 mM Tris pH 8.0, 0.1 mM EDTA buffer was added to each well at 20 uL per well. Samples were then analyzed by qPCR on the Fluidigm Biomark instrument using 96.96 chips according to manufacturer’s instructions (Fluidigm). Briefly, 2.25 uL per well of each amplified, diluted single cell sample was mixed with 2.5 uL Ssofast 2X Probes Supermix (Bio-rad) that had 0.5X ROX reference dye added (from the CellsDirect Kit, Life Technologies), and 0.25 uL sample loading reagent (Fluidigm). Taqman assays (20x concentration) were combined 1:1 with Assay loading reagent (Fluidigm). Sample and assay mixes were then added to the wells of the 96.96 chip for gene expression measurement. The normal speed cycling gene expression protocol was used, with 40 cycles of amplification. Data was exported from Fluidigm Real-time PCR Analysis software version 3.1.3, using Linear (Derivative) Baseline method, a global threshold of 0.01, and a 0.65 quality threshold, parameters which were found to exclude non-specific amplification and reduce plate-plate variation.

extract_protocol: Harvested macrophages were incubated on ice in PBS containing 5% human AB serum for 15 minutes, followed by surface antibody staining for 30 minutes on ice, in the same buffer, with the following antibody cocktail: FITC anti-CD36, clone 5-271 (Biolegend); PE anti-CD163, clone GHI/61 (Biolegend); PerCP anti-CD16, clone 3G8 (Biolegend); PE-Cy7 anti-CD197, clone G043H7 (Biolegend); APC-Cy7 anti-CD206, clone 15-2 (Biolegend); Alexa Fluor 700 anti-CD195, clone HEK/1/85a (Biolegend); Alexa Fluor 647 anti-CD192, clone TG5/CCR2 (Biolegend); Pacific Orange anti-CD14, clone TüK4 (Life Technologies). Following staining, cells were washed once with ice cold PBS, and resuspended in ice cold PBS containing a 1 in 1000 dilution of Pacific Blue Fixable Live/Dead stain (Life Technologies), which is a fluorescent dye reactive with cellular amines. When the cell membrane becomes permeable, the dye gains access to intracellular amines and hence the fluorescence of the cell increases. For sorting, cells were gated upon Forward scatter (FSC)-width versus side scatter(SSC)-area for doublet discrimination, followed by gating on Pacific blue low (membrane intact) cells (versus FSC-area). A 100 μm nozzle was used at 20 PSI pressure and a drop drive frequency of 34.5 kHz. Sorting was performed on a FACSAriaII (Becton Dickinson) and FACSDiva version 6.1.3 software using Single cell precision mode with default parameters (Yield mask = 0, Purity mask = 32, Phase mask = 16). Sort alignment was checked immediately before sorting, by sorting beads onto a covered PCR plate and confirming that the droplets land in the center of several different wells across the plate. Cells were deposited into individual wells of a low-profile 96 well PCR plate (Bio-rad) containing 2.5 uL CellsDirect 2x Reaction Mix (Life Technologies), 0.05 uL Ambion SUPERase-In RNAse inhibitor (Life technologies), and 1 uL RNAse-free water. Cells from different treatment groups were sorted into alternating wells across the plate, to avoid positional or “edge” effects across the plate. Each plate contained a no template control well, and a seven point dilution of a pooled RNA sample derived from a combination of RNA isolated from non-treated, IFNγ, and IL-4 treated macrophages, as a reference positive control. Control RNA was isolated using the miRNeasy mini kit (Qiagen). All wells except the positive control wells also contained ERCC RNA reference standards (Life Technologies) spiked in at 1/50,000 final dilution from the stock ERCC mixture.


title: p5AdJul9 24hr MCSF IL4 D3


title: p5AdJul9 24hr MCSF IL4 D11


title: p5AdJul9 24hr MCSF IL4 H3


title: p5AdJul9 24hr MCSF IL4 H7


title: p5AdJul9 24hr MCSF IL4 E8


title: p5AdJul9 24hr MCSF IL4 F5


title: p5AdJul9 24hr MCSF IL4 F9


title: p5AdJul9 24hr MCSF IL4 E4


title: p5AdJul9 24hr MCSF IL4 G10


title: p5AdJul9 24hr MCSF IL4 G6


title: p5AdJul9 24hr MCSF IL4 G2


title: p5AdJul9 24hr MCSF IL4 H11


title: p1dJul9.10cell 24hr MCSF IL10 10 B4

source_name: p1dJul9.10cell 24hr MCSF IL10 10 B4


title: p1dJul9.10cell 24hr MCSF IL10 10 B8

source_name: p1dJul9.10cell 24hr MCSF IL10 10 B8


title: p5AdJul9 24hr MCSF IL4 C6


title: p5AdJul9 24hr MCSF IL4 C10


title: p5AdJul9 24hr MCSF IL4 C2


title: p5AdJul9 24hr MCSF IL4 B5


title: p5AdJul9 24hr MCSF IL4 D7


title: p5AdJul9 24hr MCSF IL4 B9


title: p5AdJul9 24hr MCSF IL4 A8


title: p5AdJul9 24hr MCSF IL10 H10


title: p5AdJul9 24hr MCSF IL10 G7


title: p5AdJul9 24hr MCSF IL4 A12


title: p5AdJul9 24hr MCSF IL10 H2


title: p5AdJul9 24hr MCSF IL10 F8


title: p5AdJul9 24hr MCSF IL10 G11


title: p5AdJul9 24hr MCSF IL10 G3


title: p5AdJul9 24hr MCSF IL10 F4


title: p5AdJul9 24hr MCSF IL10 C3


title: p5AdJul9 24hr MCSF IL10 C7


title: p5AdJul9 24hr MCSF IL10 F12


title: p5AdJul9 24hr MCSF IL10 E9


title: p5AdJul9 24hr MCSF IL10 B12


title: p5AdJul9 24hr MCSF IL10 C11


title: p5AdJul9 24hr MCSF IL10 H6


title: p5AdJul9 24hr MCSF IL10 B8


title: p5AdJul9 24hr MCSF IL10 D10


title: p5AdJul9 24hr MCSF IL10 D6


title: p5AdJul9 24hr MCSF IL10 D2


title: p5AdJul9 24hr MCSF IL10 B4


title: p5AdJul9 24hr MCSF IL10 E5


title: p5AdJul9 24hr MCSF IL10 A9


title: p4dJul9 24hr MCSF IL4 G6


title: p4dJul9 24hr MCSF IL4 H3


title: p4dJul9 24hr MCSF IL4 H11


title: p4dJul9 24hr MCSF IL4 H7


title: p4dJul9 24hr MCSF IL4 F5


title: p4dJul9 24hr MCSF IL4 G10


title: p4dJul9 24hr MCSF IL4 F9


title: p4dJul9 24hr MCSF IL4 G2


title: p4dJul9 24hr MCSF IL4 E4


title: p4dJul9 24hr MCSF IL4 E8


title: p4dJul9 24hr MCSF IL4 D7


title: p4dJul9 24hr MCSF IL4 D3


title: p4dJul9 24hr MCSF IL4 B5


title: p4dJul9 24hr MCSF IL4 B9


title: p4dJul9 24hr MCSF IL4 A12


title: p4dJul9 24hr MCSF IL10 H2


title: p4dJul9 24hr MCSF IL10 H10


title: p4dJul9 24hr MCSF IL10 G3


title: p4dJul9 24hr MCSF IL10 G7


title: p4dJul9 24hr MCSF IL4 C10


title: p4dJul9 24hr MCSF IL4 C6


title: p4dJul9 24hr MCSF IL4 A8


title: p4dJul9 24hr MCSF IL10 H6


title: p4dJul9 24hr MCSF IL4 C2


title: p4dJul9 24hr MCSF IL4 D11


title: p4dJul9 24hr MCSF IL4 E12


title: p4dJul9 24hr MCSF IL10 F8


title: p4dJul9 24hr MCSF IL10 G11


title: p4dJul9 24hr MCSF IL10 F4


title: p4dJul9 24hr MCSF IL10 F12


title: p4dJul9 24hr MCSF IL10 D10


title: p4dJul9 24hr MCSF IL10 C7


title: p4dJul9 24hr MCSF IL10 C3


title: p4dJul9 24hr MCSF IL10 C11


title: p4dJul9 24hr MCSF IL10 D2


title: p4dJul9 24hr MCSF IL10 E9


title: p4dJul9 24hr MCSF IL10 D6


title: p4dJul9 24hr MCSF IL10 E5


title: p4dJul9 24hr MCSF IL10 B12


title: p4dJul9 24hr MCSF IL10 B4


title: p4dJul9 24hr MCSF IL10 A9


title: p4dJul9 24hr MCSF IL10 B8


title: p2dJul9 24hr MCSF IL4 H7


title: p2dJul9 24hr MCSF IL4 G10


title: p2dJul9 24hr MCSF IL4 F9


title: p2dJul9 24hr MCSF IL4 G2


title: p2dJul9 24hr MCSF IL4 H11


title: p2dJul9 24hr MCSF IL4 H3


title: p2dJul9 24hr MCSF IL4 G6


title: p2dJul9 24hr MCSF IL4 F5


title: p2dJul9 24hr MCSF IL4 D3


title: p2dJul9 24hr MCSF IL4 D7


title: p2dJul9 24hr MCSF IL4 E12


title: p2dJul9 24hr MCSF IL4 D11


title: p2dJul9 24hr MCSF IL4 C2


title: p2dJul9 24hr MCSF IL4 C10


title: p2dJul9 24hr MCSF IL4 E8


title: p2dJul9 24hr MCSF IL4 E4


title: p2dJul9 24hr MCSF IL4 C6


title: p2dJul9 24hr MCSF IL10 E9


title: p2dJul9 24hr MCSF IL10 E5


title: p2dJul9 24hr MCSF IL4 A12


title: p2dJul9 24hr MCSF IL4 B9


title: p2dJul9 24hr MCSF IL4 B5


title: p2dJul9 24hr MCSF IL10 F4


title: p2dJul9 24hr MCSF IL10 F8


title: p2dJul9 24hr MCSF IL10 F12


title: p2dJul9 24hr MCSF IL10 H2


title: p2dJul9 24hr MCSF IL10 H6


title: p2dJul9 24hr MCSF IL10 H10


title: p2dJul9 24hr MCSF IL10 G3


title: p2dJul9 24hr MCSF IL10 D6


title: p2dJul9 24hr MCSF IL10 B4


title: p2dJul9 24hr MCSF IL10 C11


title: p2dJul9 24hr MCSF IL10 D2


title: p2dJul9 24hr MCSF IL10 B8


title: p2dJul9 24hr MCSF IL10 B12


title: p2dJul9 24hr MCSF IL10 A9


title: p1dJul9 24hr MCSF IL4 G2


title: p2dJul9 24hr MCSF IL10 C3


title: p2dJul9 24hr MCSF IL4 A8


title: p2dJul9 24hr MCSF IL10 C7


title: p1dJul9 24hr MCSF IL4 G6


title: p1dJul9 24hr MCSF IL4 H3


title: p1dJul9 24hr MCSF IL4 H11


title: p1dJul9 24hr MCSF IL4 H7


title: p1dJul9 24hr MCSF IL4 F5


title: p1dJul9 24hr MCSF IL4 F9


title: p1dJul9 24hr MCSF IL4 D11


title: p1dJul9 24hr MCSF IL4 D3


title: p1dJul9 24hr MCSF IL4 E4


title: p1dJul9 24hr MCSF IL4 E12


title: p1dJul9 24hr MCSF IL4 B9


title: p1dJul9 24hr MCSF IL4 E8


title: p1dJul9 24hr MCSF IL4 G10


title: p1dJul9 24hr MCSF IL4 D7


title: p1dJul9 24hr MCSF IL4 A8


title: p1dJul9 24hr MCSF IL10 H6


title: p1dJul9 24hr MCSF IL4 B5


title: p1dJul9 24hr MCSF IL10 G7


title: p1dJul9 24hr MCSF IL10 H2


title: p1dJul9 24hr MCSF IL10 G3


title: p1dJul9 24hr MCSF IL4 C2


title: p1dJul9 24hr MCSF IL10 G11


title: p1dJul9 24hr MCSF IL4 C10


title: p1dJul9 24hr MCSF IL10 F4


title: p1dJul9 24hr MCSF IL4 C6


title: p1dJul9 24hr MCSF IL10 D6


title: p1dJul9 24hr MCSF IL10 E5


title: p1dJul9 24hr MCSF IL10 C7


title: 150131_S20_IL10__cATF2ChIP

type: SRA

description: ATF2 DNA binding analysis via ChIP-seq of human blood derived macrophages treated with/without IL-10 for 24 hours.

library_source: genomic

data_processing:
Covert bcl to fastq: CASAVA 1.8.2
Mapping: Bowtie 2.2.2
Genome_build: UCSC Hg19
Supplementary_files_format_and_content: Tab delmited text files containing read counts mapping to gene regions (RNAseq), or peak regions (ChIPseq and CAGE); Bed files with peak regions used in the read counting for CHIPseq and CAGE.

molecule: genomic DNA

source_name: Peripheral blood monocyte-derived macrophages

extract_protocol:
10 million macrophages per sample were cultured in 10 cm culture dishes, treated with or without IL-10 (10 ng/mL) for 24 hours. Following treatment incubation, Plates were rinsed once with room temperature PBS, and fixed using 1% formaldehyde for 10 minutes on an orbital shaking platform set to slowly mix the fluid. The fixation reaction was then quenched by adding glycine to a final concentration of 0.15 M and incubating a further 5 minutes. Plates were then rinsed 1x with ice-cold PBS containing protease inhibitors and harvest from the plate by scraping. Cells were centrifuged in 15 mL conical tubes 5 minutes at 300 x G at 4°C.
Immunoprecipitation was carried out as following the method of Blecher-Gonen (Blecher-Gonen et al., Nat Protoc. 2013 Mar;8(3):539-54.), with the following modifications: sonication was performed using a Covaris S2 instrument in 1 mL tubes, 20 million cells per tube, intensity 5, duty cycle 20%, 200 cycles per burst for 6 minutes; 75 μl Protein G microbeads (Invitrogen) and 3 ug of  H3K27ac antibody (Abcam ab 4729) were used. Illumina sequencing libraries were prepared using the NEBNext ChIPseq library prep kit (NEB).

treatment_protocol: Fresh media on day 6 was also supplemented with 100 ng/mL Interferon (IFN)γ and 100 ng/mL Tumor necrosis factor α (IFNγ and TNFα treated cells), 100 ng/mL Interleukin 4 (IL-4 treated cells), 10 ng/mL IL-10 (IL-10 treated cells) (cytokines from Peprotech), or M-CSF alone (Control). Cells were harvested for analysis 18 or 24 hours after treatment.

tag: treatment time (hours)

tag: replicate

value: Not applicable

tag: chip target

value: ATF2 (C-terminus)

tag: library type


title: p1dJul9 24hr MCSF IL10 E9


title: 140822_S15_CNT___

description:
5' capped RNA
Transcriptome analysis via CAGE (5' cap of RNA capture) of human blood derived macrophages treated with/without IL-10 for 24 hours.
cage_counts_forGEO.txt
mac_enhancers.bed

library_source: transcriptomic

data_processing:
Covert bcl to fastq: CASAVA 1.8.2
Adapter trimming: cutadapt 1.5
Mapping: Bowtie 2.2.2
Bidirectional enhancer regions calling and read counting: FANTOM5 methodology (scripts downloaded from https://github.com/anderssonrobin/enhancers on Aug 28, 2014; regions called using pooled data from all CAGE samples, whereas reads per region subsequently counted separately for each sample).
Differential activity analysis: DESeq2 (using all CAGE samples paired by donor)
Genome_build: UCSC Hg19
Supplementary_files_format_and_content: Tab delmited text files containing read counts mapping to gene regions (RNAseq), or peak regions (ChIPseq and CAGE); Bed files with peak regions used in the read counting for CHIPseq and CAGE.

extract_protocol:
Trizol, Zymo Direct-zol spin column
Cap-analysis of Gene Expression (CAGE) libraries were made following the method of Takahashi et al. (Takahashi et al., 2012. Nat Protoc 7, 542-561). Briefly, 1-5 ug of RNA is reverse transcribed, followed by chemical biotinylation of the 5’ cap. Following selection of capped RNA:cDNA hybrids, linkers are ligated that allow second strand cDNA synthesis and provides a recognition site (in conjunction with the site included in the RT primer) for the EcoP15I restriction enzyme, which is used to cleave the fragments 27 nucleotide downstream of the recognition site. Following further processing, these 27 nucleotide “CAGE tags” are then sequenced, and represent the 5’ ends of the RNA, including enhancer RNAs.

value: CAGE


title: 140822_S16_CNT___


title: p1dJul9 24hr MCSF IL10 F8


title: 140822_S12_CNT___


title: 150131_S20_IL10__cATF2ChIPInput

value: Input


title: 140822_S16_IL10___


title: 140715_S15_IL10__H3K27ac_

description:
Histone H3K27 acetylation analysis via ChIP-seq of human blood derived macrophages treated with/without IL-10 for 24 hours.
h3k27ac_counts_forGEO.txt
union_H3K27ac_peakslax.bed

data_processing:
Covert bcl to fastq: CASAVA 1.8.2
Mapping: Bowtie 2.2.2
Peak calling: HOMER v4.6 (union of peaks called separately in each ChIP-Input sample pair merged using bedtools merge)
Read counting: htseq-count using the merged peak file (union_H3K27ac_peakslax.bed) defined regions
Differential activity analysis: DESeq2 (using all H3K27ac IP samples paired by donor)
Genome_build: UCSC Hg19
Supplementary_files_format_and_content: Tab delmited text files containing read counts mapping to gene regions (RNAseq), or peak regions (ChIPseq and CAGE); Bed files with peak regions used in the read counting for CHIPseq and CAGE.


title: 140822_S12_IL10___


title: 140715_S15_CNT__Input_


title: p1dJul9 24hr MCSF IL10 C3


title: 140715_S12_IL10__Input_


title: p1dJul9 24hr MCSF IL10 D10


title: 140715_S15_CNT__H3K27ac_


title: p1dJul9 24hr MCSF IL10 B8


title: p1dJul9 24hr MCSF IL10 B4


title: p1dJul9 24hr MCSF IL10 A9


title: p1dJul9 24hr MCSF IL10 F12


title: 140715_S12_IL10__H3K27ac_


title: 140715_S12_CNT__Input_


title: 140715_S12_CNT__H3K27ac_


title: p1dJul9 24hr MCSF IL10 B12


title: p1dJul9 24hr MCSF IL10 C11


title: 140822_S15_IL10___


title: 140825_S2_IL10___

description:
Transcriptome analysis via RNAseq of human blood derived macrophages treated with/without IL-10 for 18 hours.
IL10_RNAseq_countsForGEO.txt

data_processing:
Covert bcl to fastq: CASAVA 1.8.2
Mapping: Tophat 2.0.11, Bowtie 2.2.2
Read counting: featureCounts (SubRead package), using gencode v19 annotation
Differential expression analysis: DESeq2
Genome_build: UCSC Hg19
Supplementary_files_format_and_content: Tab delmited text files containing read counts mapping to gene regions (RNAseq), or peak regions (ChIPseq and CAGE); Bed files with peak regions used in the read counting for CHIPseq and CAGE.

extract_protocol:
Trizol, Zymo Direct-zol spin column
Illumina Truseq Ribozero Stranded HT

value: RNAseq (Ribozero Stranded Truseq HT)


title: 140825_S2_CNT___


title: 140715_S15_IL10__Input_


title: 140825_S2_IFNgTNFa___

description: Transcriptome analysis via RNAseq of human blood derived macrophages treated with/without IL-10 for 18 hours.

data_processing:
Covert bcl to fastq: CASAVA 1.8.4
Mapping: Tophat 2.0.11, Bowtie 2.2.4
Read counting: featureCounts (SubRead package), using gencode v19 annotation
Differential expression analysis: DESeq2
Genome_build: UCSC Hg19
Supplementary_files_format_and_content: Tab delmited text files containing read counts mapping to gene regions (RNAseq), or peak regions (ChIPseq and CAGE); Bed files with peak regions used in the read counting for CHIPseq and CAGE.

value: IFNgTNFa


title: 140825_S3_IFNgTNFa___

data_processing:
Covert bcl to fastq: CASAVA 1.8.5
Mapping: Tophat 2.0.11, Bowtie 2.2.5
Read counting: featureCounts (SubRead package), using gencode v19 annotation
Differential expression analysis: DESeq2
Genome_build: UCSC Hg19
Supplementary_files_format_and_content: Tab delmited text files containing read counts mapping to gene regions (RNAseq), or peak regions (ChIPseq and CAGE); Bed files with peak regions used in the read counting for CHIPseq and CAGE.


title: p1dJul9 24hr MCSF IL10 D2


title: 140825_S3_CNT___


title: 140825_S1_IL10___


title: 140825_S1_IFNgTNFa___

data_processing:
Covert bcl to fastq: CASAVA 1.8.3
Mapping: Tophat 2.0.11, Bowtie 2.2.3
Read counting: featureCounts (SubRead package), using gencode v19 annotation
Differential expression analysis: DESeq2
Genome_build: UCSC Hg19
Supplementary_files_format_and_content: Tab delmited text files containing read counts mapping to gene regions (RNAseq), or peak regions (ChIPseq and CAGE); Bed files with peak regions used in the read counting for CHIPseq and CAGE.


title: 140421_S2_IL10_A__

value: A


title: 140421_S2_IL10_B__

value: B


title: 140421_S2_CNT_A__


title: 140825_S1_CNT___


title: 140825_S3_IL10___


title: Tumor9

status: Public on Mar 01 2017

type: genomic

city: Wien

first: Gerwin

last: Heller

address: Währinger Gürtel 18-20

department: Medicine I

country: Austria

institute: Medical University of Vienna

hyb_protocol: Accordingto NimbleGen protocols and performed by Nimblegen Service Laboratory (Reykjavik, Iceland)

data_processing:
Arrays were processed using Nimblegen's standard protocol for Nimblescan 2.5 MeDIP-chip data extraction.
the P value derived from Kolmogorov-Smirnov test using 750bp window size of the Cy5/Cy3 log2 ratio using NimbleScan.

label_protocol: Input DNA was labeled with Cy3 and MeDIP DNA was labeled with Cy5 by Nimblegen Service Laboratory (Reykjavik, Iceland)

extract_protocol: DNA isolation was performed by proteinase K digestion followed by standard phenol/chloroform extraction and ethanol precipitation. MeDIP was performed according to NimbleGen´s "Sample preparation protocol" using Eurogentech´s anti 5-methylcytosine antibody. Enrichment of methylated DNA fragments was tested by qRT-PCR comparing H19 and GAPDH levels.

tag: subject status

value: non-small-cell lung cancer (NSCLS) patient

tag: subject id/no.

tag: tissue type

value: lung tumor tissue

tag: medip antibody

value: Anti-5-mythylcytidine antibody from Eurogentech (BI-MECY-0100)


title: 140421_S2_CNT_B__


title: Tumor49

scan_protocol: Accordingto NimbleGen protocols and performed by Nimblegen Service Laboratory (Reykjavik, Iceland)


title: Tumor8


title: Tumor48


title: Tumor47


title: Tumor50


title: Tumor7


title: Tumor6


title: Tumor44


title: Tumor5


title: Tumor43


title: Tumor45


title: Tumor42


title: Tumor41


title: Tumor29


title: Tumor40


title: Tumor4


title: Tumor39


title: Tumor38


title: Tumor25


title: Tumor36


title: Tumor23


title: Tumor24


title: Tumor22


title: Tumor35


title: Tumor37


title: Tumor26


title: Tumor46


title: Tumor34


title: Tumor32


title: Tumor3


title: Tumor30


title: Tumor33


title: Tumor28


title: Tumor27


title: Tumor21


title: Tumor18


title: Tumor16


title: Tumor2


title: Tumor31


title: Tumor19


title: Tumor20


title: Tumor15


title: Tumor10


title: Tumor14


title: Tumor12


title: Tumor13


title: Normal9

value: non-malignant lung tissue


title: Normal50


title: Normal8


title: Tumor1


title: Tumor17


title: Normal5


title: Tumor11


title: Normal6


title: Normal47


title: Normal43


title: Normal45


title: Normal4


title: Normal39


title: Normal41


title: Normal37


title: Normal7


title: Normal49


title: Normal38


title: Normal35


title: Normal34


title: Normal46


title: Normal30


title: Normal32


title: Normal44


title: Normal26


title: Normal27


title: Normal28


title: Normal31


title: Normal29


title: Normal48


title: Normal25


title: Normal24


title: Normal22


title: Normal40


title: Normal3


title: Normal21


title: Normal20


title: Normal17


title: Normal2


title: Normal16


title: Normal19


title: Normal15


title: Normal18


title: Normal33


title: Normal14


title: Normal13


title: Normal11


title: Normal23


title: Normal10


title: Normal12


title: Normal1


title: Normal42


title: S105-5

status: Public on Jan 12 2017

city: Durham

first: Trudy

last: Oliver

email: tgo@duke.edu, trudy.oliver@duke.edu

state: NC

address: Duke University, Box 3813, LSRC Room C138B, 308 Research Drive

department: Pharmacology & Cancer Biology

institute: Duke University

data_processing:
Mouse mm10 annotations (Ensembl build 82) were used in the RSEM (v1.2.12) utility rsem-prepare-reference to create bowtie (v1.0.1) indices.
Gene expression was determined using the RSEM utility rsem-calculate-expression with the forward strand probability set to zero.
Differential expression was determined using EBSeq (v1.4.0) using ‘MedianNorm’ function to calculate size factors and setting ‘maxround’ to 10.
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text file containing FPKM values

organism: Mus musculus

source_name: mouse small cell lung cancer tumor tissue from Rbfl/fl p53fl/fl P130 fl/fl mice

extract_protocol:
RNA was extracted from tumors using RNeasy Kit (Qiagen, Valencia,CA,USA).
The RNA-seq libraries were generated using TruSeq Stranded mRNA sample preparation kit (Illumina, San Diego, CA, USA) with oligo dT selection. Sequencing libraries (25 pM) were chemically denatured and applied to an Illumina HiSeq v4 paired end flow cell using an Illumina cBot. Hybridized molecules were clonally amplified and annealed to sequencing primers with reagents from an Illumina HiSeq PE Cluster Kit v4-cBot

tag: tissue

value: lung, small cell lung cancer tumor sample, untreated

tag: genotype


title: PB190C

source_name: mouse small cell lung cancer tumor tissue from Rbfl/fl p53fl/fl Myc (T58A) LSL/LSL mice

value: RPM


title: PB281E


title: PB191A


title: S100-12


title: PB190A


title: PB119

source_name: mouse small cell lung cancer tumor tissue from Rbfl/fl p53fl/fl Myc (T58A) LSL/+ mice


title: PB189B


title: PB280H


title: S90-71


title: PB120


title: PB124


title: PB281F


title: PB115


title: Normal36


title: CRPS 040B

status: Public on Dec 01 2016

city: Izumo

first: Jun

last: Yoshino

state: Shimane

address: 89-1 Enya-cho

country: Japan

institute: Faculty of Medicine, Shimane University

description:
24874
After 10% weight loss induced by a normal-protein weight loss diet

hyb_protocol: Following fragmentation with NuGen Encore Biotin Module,2.5 ug of cDNA were hybridized into the GeneChip Hybridization Oven 640 for 18 hr at 45C on Gene 1.0 ST Array. GeneChip were washed and stained in the Affymetrix Fluidics Station 450.

scan_protocol: GeneChips were scanned using the Affymetrix GeneChip 7G 3000 Scanner.

data_processing: The data was analyzed with the Affymetrix GeneChip Command Console. Microarray expression data was processed using Command Console (Affymetrix, Inc) and the raw (.CEL) files generated were analyzed using Expression Console software with Affymetrix default RMA Gene analysis settings.(Affymetrix, Inc).  Probe summarization (Robust Multichip Analysis, RMA), quality control analysis, and probe annotation were performed according to recommended guidelines (Expression Console Software v.1.4, Affymetrix, Inc.).

label: Biotin

source_name: After WL_muscle

label_protocol: Biotinylated cDNA were prepared according to the standard NuGen WT-Ovation Pico RNA Amplification system from 500pg-50 ng total RNA.

growth_protocol: Skeletal muscle gene expression was examined in people who are obese before and after 10% weight loss induced by normal protein and high protein dietary intervention.

extract_protocol: Skeletal muscle biopsies were obtained and immediately frozen in liquid nitrogen. Total RNA was isolated from frozen tissue sample by using Trizol (Invitrogen).

treatment_protocol: After baseline studies, subjects were randomized to one of three dietary intervention groups: 1) a weight maitenance group, 2) a weight loss group (normal protein intake, 0.8 g protein/kg body weight per day) and 3) a weight loss group (high protein intake, 1.2 g protein/kg body weight per day). Skeletal muscle samples were obtained during the hyperinsulinemic euglycemic clamp procedure before and after subjects lost 10% of their body weight.

value: postmenopausal women who were obese

tag: subject id

value: CRPS 040

tag: dietary intervention group

value: weight loss group with normal protein intake (WL)

tag: time point

value: After WL

value: skeletal muscle


title: CRL 025A

description:
24885
Before 10% weight loss induced by a high-protein weight loss diet

source_name: Before WL-HP_muscle

value: CRL 025

value: weight loss group with high protein intake (WL-HP)

value: Before WL-HP


title: CRPS 040A

description:
24873
Before 10% weight loss induced by a normal-protein weight loss diet

source_name: Before WL_muscle

value: Before WL


title: CRL 025B

description:
24886
After 10% weight loss induced by a high-protein weight loss diet

source_name: After WL-HP_muscle

value: After WL-HP


title: CRL 016B

description:
24882
After 10% weight loss induced by a high-protein weight loss diet

value: CRL 016


title: CRL 003B

description:
24878
After 10% weight loss induced by a high-protein weight loss diet

value: CRL 003


title: CRL 002A

description:
24875
Before 10% weight loss induced by a normal-protein weight loss diet

value: CRL 002


title: CRL 016A

description:
24881
Before 10% weight loss induced by a high-protein weight loss diet


title: CRL 013A

description:
24879
Before 10% weight loss induced by a normal-protein weight loss diet

value: CRL 013


title: CRL 013B

description:
24880
After 10% weight loss induced by a normal-protein weight loss diet


title: CRPS 019A

description:
24867
Before 10% weight loss induced by a normal-protein weight loss diet

value: CRPS 019


title: CRPS 022B

description:
24870
After 10% weight loss induced by a high-protein weight loss diet

value: CRPS 022


title: CRPS 007B

description:
24864
After 10% weight loss induced by a high-protein weight loss diet

value: CRPS 007


title: CRL 020A

description:
24883
Before 10% weight loss induced by a high-protein weight loss diet

value: postmenopausal women who were obese

value: CRL 020


title: CRL 020B

description:
24884
After 10% weight loss induced by a high-protein weight loss diet


title: CRPS 007A

description:
24863
Before 10% weight loss induced by a high-protein weight loss diet


title: CRL 002B

description:
24876
After 10% weight loss induced by a normal-protein weight loss diet

value: weight loss group with normal protein intake (WL)


title: CRPS 024B

description:
24872
After 10% weight loss induced by a normal-protein weight loss diet

value: CRPS 024

value: After WL


title: CRPS 022A

description:
24869
Before 10% weight loss induced by a high-protein weight loss diet

value: skeletal muscle


title: CRL 003A

description:
24877
Before 10% weight loss induced by a high-protein weight loss diet

value: weight loss group with high protein intake (WL-HP)


title: CRPS 024A

description:
24871
Before 10% weight loss induced by a normal-protein weight loss diet


title: CRPS 012A

description:
24865
Before 10% weight loss induced by a high-protein weight loss diet

value: CRPS 012

value: Before WL-HP


title: CRPS 004A

description:
24861
Before 10% weight loss induced by a normal-protein weight loss diet

value: CRPS 004

value: Before WL


title: CRPS 012B

description:
24866
After 10% weight loss induced by a high-protein weight loss diet

value: After WL-HP


title: CRPS 003B

description:
24860
After 10% weight loss induced by a high-protein weight loss diet

value: CRPS 003


title: CRPS 004B

description:
24862
After 10% weight loss induced by a normal-protein weight loss diet


title: CRPS 003A

description:
24859
Before 10% weight loss induced by a high-protein weight loss diet


title: CRPS 019B

description:
24868
After 10% weight loss induced by a normal-protein weight loss diet


title: CRPS 001B

description:
24858
After 10% weight loss induced by a high-protein weight loss diet

value: CRPS 001


title: CRPS 001A

description:
24857
Before 10% weight loss induced by a high-protein weight loss diet


title: WAT, control, 6M, rep2

hyb_protocol: Standard Illumina hybridization protocol

scan_protocol: Standard Illumina scanning protocol

data_processing:
The background-subtracted raw microarray data were subjected to the quantile normalization and false discovery rates (FDR) were calculated using the Rank Products Analysis. The background-subtracted raw microarray data were also normalized by Z score transformation to obtain Z ratios. All data were analyzed by the R statistical software package.
Z scores/ratios were obtained with Z score transformation.  Z socres are shown in the matrix data.

label: biotin

label_protocol: Biotinylated cRNA were prepared for Illumina arrays

growth_protocol: C57BL/6N male mice (Taconic, NY) were group-housed in a barrier facility with 12-h light/12-h dark cycles.  All mice received a regular chow diet ad libitum (PicoLab 5053 Rodent Diet 20; Lab Diets).

extract_protocol: Total RNA was isolated from frozen skeletal muscle, white adipose tissue and liver samples, by using the PureLink™ RNA Mini Kit (Cat. no. 12183018A; Invitrogen, Carlsbad, CA).

treatment_protocol: NMN was orally given to mice for 6 to 12 months.

tag: strain

tag: gender

value: male

tag: treated with

value: none (control) for 6 months

value: WAT


title: Liver, NMN, 12M, rep1

value: 300 mg/kg/day Nicotinamide mononucleotide (NMN) for 12 months

value: Liver


title: Liver, NMN, 6M, rep1

value: 300 mg/kg/day Nicotinamide mononucleotide (NMN) for 6 months


title: WAT, NMN, 6M, rep4


title: Liver, NMN, 6M, rep4


title: WAT, NMN, 12M, rep2


title: Liver, control, 12M, rep1

value: none (control) for 12 months


title: Liver, NMN, 12M, rep2


title: WAT, control, 6M, rep3


title: WAT, NMN, 6M, rep3


title: Liver, NMN, 12M, rep4


title: WAT, NMN, 12M, rep3


title: WAT, control, 12M, rep4


title: WAT, control, 12M, rep1


title: Liver, NMN, 6M, rep3


title: WAT, control, 12M, rep2


title: WAT, control, 12M, rep3


title: Liver, NMN, 6M, rep2


title: WAT, NMN, 6M, rep1


title: Liver, control, 6M, rep4


title: Liver, control, 12M, rep3


title: WAT, NMN, 12M, rep4


title: Liver, control, 12M, rep2


title: WAT, NMN, 12M, rep1


title: WAT, control, 6M, rep1


title: WAT, NMN, 6M, rep2


title: Liver, NMN, 12M, rep3


title: WAT, control, 6M, rep4


title: Liver, control, 12M, rep4


title: Skeletal muscle, NMN, 12M, rep4

value: Muscle


title: Skeletal muscle, NMN, 12M, rep3


title: Skeletal muscle, NMN, 12M, rep1


title: Skeletal muscle, control, 12M, rep4


title: Liver, control, 6M, rep1


title: Liver, control, 6M, rep3


title: Liver, control, 6M, rep2


title: Skeletal muscle, control, 12M, rep1


title: Skeletal muscle, control, 12M, rep2


title: Skeletal muscle, NMN, 12M, rep2


title: Skeletal muscle, control, 12M, rep3


title: Skeletal muscle, NMN, 6M, rep1


title: Skeletal muscle, NMN, 6M, rep4


title: FM114-0

status: Public on Jul 01 2016

description: Before n-3 PUFA therapy

data_processing: The data was analyzed with the Affymetrix GeneChip Command Console. Microarray expression data was processed using Command Console (Affymetrix, Inc) and the raw (.CEL) files generated were analyzed using Expression Console software with Affymetrix default RMA Gene analysis settings.(Affymetrix, Inc).  Probe summarization (Robust Multichip Analysis, RMA), quality control analysis, and probe annotation were performed according to recommended guidelines (Expression Console Software, Affymetrix, Inc.).

source_name: Thigh muscle_Before n-3 PUFA therapy

growth_protocol: Muscle gene expression was examined in a subset of 10 healthy 60-85 year old men with the largest hypertrophy response (change in thigh muscle volume).

extract_protocol: Thigh muscle biopsies were obtained and immediately frozen in liquid nitrogen. Total RNA was isolated from frozen tissue sample by using Trizol (Invitrogen).

treatment_protocol: After baseline studies, subjects were instructed to take four 1-gram pills per day providing a total of 1.86 g eicosapentaenoic acid [20:5 n-3] and 1.50 g docosahexaenoic acid [22:6 n-3] per day for 6 months.

value: muscle

tag: treatment status

value: Before

tag: age

value: 60-85 years


title: Skeletal muscle, control, 6M, rep1


title: Skeletal muscle, NMN, 6M, rep2


title: FM93-24

description: After n-3 PUFA therapy

source_name: Thigh muscle_After n-3 PUFA therapy

value: After


title: FM108-0

description: Before n-3 PUFA therapy


title: FM108-24

description: After n-3 PUFA therapy


title: FM103-24

description: After n-3 PUFA therapy


title: Skeletal muscle, NMN, 6M, rep3


title: Skeletal muscle, control, 6M, rep2


title: FM114-24

description: After n-3 PUFA therapy


title: WT

status: Public on Oct 01 2016

description: Proximal section from whole intestine (upper 1/3 of the gut) obtained from wild type mice

hyb_protocol: Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 plus 2/Mouse 430 2.0  Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.

scan_protocol: GeneChips were scanned using the Affymetrix GeneArray Scanner 7G06.

data_processing: The data were analyzed with Affymetrix GeneChip Command Console using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.

source_name: Intestine

label_protocol: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA.

growth_protocol: Germline CD36-null (CD36KO) and WT mice on the C57BL/6 background were housed in a facility with a 12-h light-dark cycle and fed chow ad libitum (Purina, St. Louis, MO, USA).

extract_protocol: RNA was extracted from 3 animals using TRIzol (Invitrogen, Carlsbad, CA) and pooled for mircoarray analysis.

treatment_protocol: Proximal section from whole intestine (upper 1/3 of the gut) was isolated for RNA extraction.

tag: genotype/variation

value: Wild type mice

value: proximal section of intestine


title: Skeletal muscle, control, 6M, rep4


title: Skeletal muscle, control, 6M, rep3


title: FM43-24

description: After n-3 PUFA therapy


title: FM54-24

description: After n-3 PUFA therapy


title: FM56-24

description: After n-3 PUFA therapy


title: FM54-0

description: Before n-3 PUFA therapy


title: FM56-0

description: Before n-3 PUFA therapy


title: FM93-0

description: Before n-3 PUFA therapy


title: FM84-0

description: Before n-3 PUFA therapy


title: FM84-24

description: After n-3 PUFA therapy


title: FM43-0

description: Before n-3 PUFA therapy


title: CD36KO

description: Proximal section from whole intestine (upper 1/3 of the gut) obtained from CD36 knockout mice

value: CD36 knockout mice


title: p4dJul9.10cell 24hr MCSF IFN+TNF 10 H8

status: Public on Jun 28 2016

source_name: p4dJul9.10cell 24hr MCSF IFN+TNF 10 H8

tag: plateid

tag: treatment time

tag: growth factor

value: MCSF

tag: treatment

value: IFN+TNF

tag: cell number

tag: well


title: FM103-0

description: Before n-3 PUFA therapy

tag: tissue

value: male


title: p4dJul9.10cell 24hr MCSF IFN+TNF 10 H12

source_name: p4dJul9.10cell 24hr MCSF IFN+TNF 10 H12


title: FM2-24

description: After n-3 PUFA therapy


title: p4dJul9.10cell 24hr MCSF IFN+TNF 10 H4

source_name: p4dJul9.10cell 24hr MCSF IFN+TNF 10 H4


title: p4dJul9.10cell 24hr MCSF IFN+TNF 10 F6

source_name: p4dJul9.10cell 24hr MCSF IFN+TNF 10 F6


title: p4dJul9.10cell 24hr MCSF IFN+TNF 10 G9

source_name: p4dJul9.10cell 24hr MCSF IFN+TNF 10 G9


title: p4dJul9.10cell 24hr MCSF IFN+TNF 10 G5

source_name: p4dJul9.10cell 24hr MCSF IFN+TNF 10 G5


title: p4dJul9.10cell 24hr MCSF IFN+TNF 10 F2

source_name: p4dJul9.10cell 24hr MCSF IFN+TNF 10 F2


title: FM1-24

description: After n-3 PUFA therapy


title: p4dJul9.10cell 24hr MCSF IFN+TNF 10 E11

source_name: p4dJul9.10cell 24hr MCSF IFN+TNF 10 E11


title: p4dJul9.10cell 24hr MCSF IFN+TNF 10 D8

source_name: p4dJul9.10cell 24hr MCSF IFN+TNF 10 D8


title: FM1-0

description: Before n-3 PUFA therapy


title: p4dJul9.10cell 24hr MCSF IFN+TNF 10 D12

source_name: p4dJul9.10cell 24hr MCSF IFN+TNF 10 D12


title: p4dJul9.10cell 24hr MCSF IFN+TNF 10 D4

source_name: p4dJul9.10cell 24hr MCSF IFN+TNF 10 D4


title: p4dJul9.10cell 24hr MCSF IFN+TNF 10 C9

source_name: p4dJul9.10cell 24hr MCSF IFN+TNF 10 C9