title: Sample 67_wt_vn_R4

data_processing:
samples 1-40: NextSeq 550 RTA software, samples 41-67: illumina NovaSeq System software
data were processed with nf-core/rnaseq (https://github.com/nf-core/rnaseq)
deviation from default pipeline options (star_salmon): parameters for trimming samples 1-40: --clip_r1 19 --clip_r2 9 --three_prime_clip_r1 5 --three_prime_clip_r2 5
counts table was analysed with Deseq2 and p-Values calculated with Wald-test
Genome_build: Tair10
Supplementary_files_format_and_content: Matrix table with raw counts for every gene and transposon for every sample

source_name: pollen

growth_protocol: for samples 1-40: seedlings were grown on soil at standard growth conditions (16h light/8h dark) and shoot apices harvested 7 days after germination. For samples 41-67, pollen was harvested accoding to (DOI: 10.1111/j.1365-313X.2004.02147.x)

extract_protocol:
for samples 1-40: nuclei were extracted according to (DOI: 10.1007/978-1-0716-0179-2_7) and per replicate 100 nuclei sorted into (SmartSeq-) lysis buffer. For samples 41-67: nuclei were extracted according to (DOI: 10.1007/s00497-015-0258-2) into Trizol and total RNA extracted.
for samples 1-40: Smart-seq3 (according to Hagemann-Jensen et. al, https://doi.org/10.1038/s41587-020-0497-0), for samples 41-67: Smart-seq2 (DOI: 10.1038/nmeth.2639) from 2ng of RNA

tag: ecotype

value: 35 days after germination

value: vegetative cells


title: Replicate 3, dura mater, wild-type, 2-month-old, female

status: Public on Sep 30 2023

type: SRA

city: Helsinki

first: Salli

last: Antila

state: Uusimaa

address: Haartmaninkatu 8

department: Faculty of Medicine

country: Finland

institute: University of Helsinki, Wihuri Research Insitute

library_source: transcriptomic single cell

data_processing:
Data processing and analysis were performed using 10x Genomics Cell Ranger v3.0.1 pipelines.
The Sample libraries were sequenced on Illumina NovaSeq 6000 system using read lengths: 28bp (Read 1), 8bp (i7 Index), 0 bp (i5 Index) and 89bp (Read 2).
Assembly: Alignment was done against mouse genome mm10.
Supplementary files format and content: Cell Ranger output files (barcodes.tsv, features.tsv, matrix.mtx)

molecule: total RNA

organism: Mus musculus

source_name: Dura mater pooled from 4 individual mice

extract_protocol:
Dorsal dura mater from four different PBS-perfused mice were pooled as one sample. Freshly collected and cut tissues were dissociated with enzymatic digestion (Collagenase VIII and DNase I) and mechanical dissociation with pippeting. Cells were washed with Ca2+ and Mg2+ free HBSS and strained with 70-µm cell strainer. DAPI- viable singlets were sorted with FACS into 1.5 ml tube and kept in PBS with 0.04% BSA.
The Chromium Single Cell 3’RNAseq run and library preparation were done using the Chromium™ Single Cell 3' Reagent version 3 chemistry according to the manufacter’s instructions.

tag: tissue

tag: cell type

value: All DAPI- viable singlets

tag: cell target in loading / number of cells to be sequenced

value: 10000 cells

tag: sequencing depth (pe/cell)

tag: genotype

value: wild-type

tag: genetic background

tag: age

value: 2.4 months / 10.4 weeks

tag: gender

value: female


title: HCC3153 cells, shRNA control

city: Oakland

first: Ravi

last: Sachidanandam

state: CA

address: 2945 Webster St

country: USA

institute: Girihlet Inc.

library_source: transcriptomic

data_processing:
Exact matches of the Reads (36 nt)  to the human genome (version hg19) were found, using a suffix-array based method. Using annotations of the genome from UCSC browser, genes were assigned counts. Longer reads in some of the experiments (72 nt), were split into two and mapped exactly.
Genome_build: hg19
Supplementary_files_format_and_content: csv file of read counts per gene (rows) in each experiment (columns)

organism: Homo sapiens

source_name: breast cancer cell line

growth_protocol: A3250, MDA-MB231, HCC3153 and cells were cultured in high-glucose DMEM supplemented with 4 mM L-glutamine (Sigma, Cat. # D6429-500ML), 10% FBS (Corning, Cat. # 35-010-CV) and 1X antibiotics (100 U/ml penicillin, 100 U/ml streptomycin). The cells were plated in 10 cm dishes two days before RNA extraction (90% confluency at the time of RNA extraction)

extract_protocol: Total RNA was isolated from samples using TRIZOL reagent (Invitrogen) and RNeasy columns (Qiagen). cDNA was synthesized using random hexamers and ligated with bar-coded adaptors compatible with Illumina's NextSeq 500 (???).

treatment_protocol: A3250 cells were transduced with the VIRHD-EP lentiviral vector and selected with blasticidin (10 ug/ml).

tag: cell line

value: Ductal breast carcinoma

tag: treatment

value: shRNA control


title: GM12878-WAPL-ChIP-seq

city: FARMINGTON

first: Yijun

last: Ruan

state: CT

address: 10 DISCOVERY DR

department: Ruan

institute: The Jackson Laboratory

description:
Sample 62
nuclear chromatin
WAPL-ChIP-seq
CHG0032.q30.nr.sorted.bedgraph
CHG0032.q30.sorted.nr.spp.z6.broadPeak

library_source: genomic

data_processing:
ChIA-Drop fastq files were mapped by longranger (version 2.1.5, 10X Genomics) with wgs command. ChIP-seq fastq files were mapped by BWA (version 0.7.7) with mem command. ChIA-PET fastq files were mapped by our in-house ChIA-PET processing pipeline, available in github (ChIA-PIPE).
After mapping and identifying barcords of ChIA-Drop using longranger, our in-house processing pipeline (available in github, ChIA-DropBox) generated putative complexes. These data sets were further filtered by MIA-Sig (available in github), and then generated tab-delimited txt file.
DNA-binding protein coverage files (bedgraph) were generated by bedtools (version 2.26.0) with genomecov command. Coverage files were sorted by chromosome and coordinate.
ChIA-PET loop files (bedpe) were generated by our in-house ChIA-PET processing pipeline, available in github (ChIA-PIPE).
Peaks (broadPeak, bed file type) were called by SPP (version 1.13) with our input control file (sample# 63, library ID: CHG0033).
2D contact map files (hic) were generated by Juicer Tools (Version 1.7.5) using chromatin interaction data from ChIA-PET and ChIA-Drop. In ChIA-Drop, we used all pairwise interactions from each GEM.
Genome_build: hg38
Supplementary_files_format_and_content: tab-delimited txt format of ChIA-Drop, containing GEM_ID, GEM_coordinate, GEM_span, Fragment_number, List_of_fragment_coordinates. Each row represents one GEM.
Supplementary_files_format_and_content: bedgraph format of ChIA-Drop, ChIP-seq, and ChIA-PET, containing a DNA-binding protein coverage
Supplementary_files_format_and_content: bedpe format of ChIA-PET, containing ChIA-PET loop (long-range interaction data) data
Supplementary_files_format_and_content: broadPeak format of ChIP-seq and ChIA-PET, containing peaks called by SPP software
Supplementary_files_format_and_content: hic format of ChIA-Drop and ChIA-PET, 2D contact map file, visualized by Juicebox software

molecule: genomic DNA

extract_protocol:
The overall chromatin sample preparation for ChIA-Drop is very similar to Hi-C, except without the proximity ligation step in ChIA-Drop.  Similarly, CTCF, cohesin, and RNAPII ChIA-Drop chromatin samples are prepared as they are for respective ChIA-PET experiments but omitting proximity ligation step.
Fragmented chromatin sample was mixed with BSA to prevent chromatin aggregation. About 0.5ng of chromatin DNA material was loaded to microfluidics device, Chromium Controller with reagents from Chromium Genome v2 Library Kit & Gel Bead Kit for partitioning of single chromatin complex into individual droplets with unique gel beads according to the manufacturer’s protocol with modification. The barcoded DNA amplicons of each chromatin complex were released from all droplets.The purified DNA fragments were subjected for end repair, A-tailing, adaptor ligation, Sample Index PCR and size selection.  The final ChIA-Drop library was sequenced (2× 150 bp) by NextSeq, HiSeq, or NovaSeq runs.
We applied microfluidics-based strategy for genome-wide analysis of multiplex chromatin interactions with single-molecule precision (ChIA-Drop). Here, individual chromatin complexes are isolated in droplets that contain a gel bead with unique DNA barcode, in which tethered chromatin DNA fragments are barcoded and amplified for sequencing and mapping to demarcate chromatin contacts.

tag: method

value: WAPL-ChIP-seq

tag: antibody

value: WAPL (Abcam, catalog# ab70741)


title: Sample 66_wt_vn_R3

city: Vienna

first: Ruben

last: Gutzat

state: Vienna

address: Dr. Bohr-Gasse 3

country: Austria

institute: Gregor Mendel Institute GmbH

molecule: nuclear RNA

organism: Arabidopsis thaliana

tag: developmental age


title: SUM-149 cells, shRNA ROR2, rep2

value: Inflammatory breast carcinoma

value: shRNA ROR2


title: A3250 cells, shRNA ROR1

value: shRNA ROR1


title: Replicate 2, dura mater, wild-type, 9-month-old, male

value: Dura mater pooled from 4 individual mice

value: 8000 cells

value: male


title: GM19239-NE-ChIA-Drop-Rep8

description:
Sample 60
nuclear chromatin
ChIA-Drop
GM19239-NE-pooled.txt
GM19239-NE-pooled.hic

data_processing:
Library strategy: ChIA-Drop
ChIA-Drop fastq files were mapped by longranger (version 2.1.5, 10X Genomics) with wgs command. ChIP-seq fastq files were mapped by BWA (version 0.7.7) with mem command. ChIA-PET fastq files were mapped by our in-house ChIA-PET processing pipeline, available in github (ChIA-PIPE).
After mapping and identifying barcords of ChIA-Drop using longranger, our in-house processing pipeline (available in github, ChIA-DropBox) generated putative complexes. These data sets were further filtered by MIA-Sig (available in github), and then generated tab-delimited txt file.
DNA-binding protein coverage files (bedgraph) were generated by bedtools (version 2.26.0) with genomecov command. Coverage files were sorted by chromosome and coordinate.
ChIA-PET loop files (bedpe) were generated by our in-house ChIA-PET processing pipeline, available in github (ChIA-PIPE).
Peaks (broadPeak, bed file type) were called by SPP (version 1.13) with our input control file (sample# 63, library ID: CHG0033).
2D contact map files (hic) were generated by Juicer Tools (Version 1.7.5) using chromatin interaction data from ChIA-PET and ChIA-Drop. In ChIA-Drop, we used all pairwise interactions from each GEM.
Genome_build: hg38
Supplementary_files_format_and_content: tab-delimited txt format of ChIA-Drop, containing GEM_ID, GEM_coordinate, GEM_span, Fragment_number, List_of_fragment_coordinates. Each row represents one GEM.
Supplementary_files_format_and_content: bedgraph format of ChIA-Drop, ChIP-seq, and ChIA-PET, containing a DNA-binding protein coverage
Supplementary_files_format_and_content: bedpe format of ChIA-PET, containing ChIA-PET loop (long-range interaction data) data
Supplementary_files_format_and_content: broadPeak format of ChIP-seq and ChIA-PET, containing peaks called by SPP software
Supplementary_files_format_and_content: hic format of ChIA-Drop and ChIA-PET, 2D contact map file, visualized by Juicebox software

value: ChIA-Drop

value: HindIII fragmented chromatin, including all chromatin associated proteins


title: GM12878-NIPBL-ChIP-seq

description:
Sample 61
nuclear chromatin
NIPBL-ChIP-seq
CHG0030.q30.nr.sorted.bedgraph
CHG0030.q30.sorted.nr.spp.z6.broadPeak

value: NIPBL-ChIP-seq

value: NIPBL (Bethyl Laboratories, catalog# A301-779A)


title: Replicate 1, dura mater, wild-type, 14-month-old, female

value: All DAPI- viable singlets

value: wild-type


title: A3250 cells, shRNA Control

value: shRNA Control


title: H2O2_SB431542_3

city: Kyoto

first: Kenta

last: Yamamoto

address: Kajicho 465

department: Immunology

country: Japan

institute: Kyoto Prefectural University of Medicine

data_processing:
strand NGS was used.
Assembly: GRCm39
Supplementary files format and content: Raw read count values and normalized values for each sample.

source_name: H2O2_SB431542-treated mice

extract_protocol:
RNA was isolated using the ISOGENⅡ（NIPPON GENE CO., LTD） and processed as per manufacturer’s instructions.
Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.

value: total bladder

tag: background strain

value: intravesical H2O2 infusion and intraperitoneal injection of TGFBeta inhibitor


title: Sample 64_wt_vn_R1

value: 35 days after germination

value: vegetative cells


title: Sample 63_wt_sperm_R3

value: sperm cells


title: H2O2_SB431542_2


title: GM19239-NE-ChIA-Drop-Rep6

description:
Sample 58
nuclear chromatin
ChIA-Drop
GM19239-NE-pooled.txt
GM19239-NE-pooled.hic


title: GM19239-NE-ChIA-Drop-Rep7

description:
Sample 59
nuclear chromatin
ChIA-Drop
GM19239-NE-pooled.txt
GM19239-NE-pooled.hic


title: H2O2_SB431542_1


title: Sample 65_wt_vn_R2


title: GM12878-RNAPII-ChIA-PET-Rep1

description:
Sample 67
nuclear chromatin
RNAPII-ChIA-PET
LHG0035N_0035V_0045V.e500.clusters.cis.BE3
LHG0035N_0035V_0045V.for.BROWSER.sorted.bedgraph
LHG0035N_0035V_0045V.for.BROWSER.spp.z6.broadPeak
ChIA-PET_hg38_GM12878_RNAPII_LHG0035N_0035V_0045V_pooled_pairs.hic

data_processing:
Library strategy: ChIA-PET
ChIA-Drop fastq files were mapped by longranger (version 2.1.5, 10X Genomics) with wgs command. ChIP-seq fastq files were mapped by BWA (version 0.7.7) with mem command. ChIA-PET fastq files were mapped by our in-house ChIA-PET processing pipeline, available in github (ChIA-PIPE).
After mapping and identifying barcords of ChIA-Drop using longranger, our in-house processing pipeline (available in github, ChIA-DropBox) generated putative complexes. These data sets were further filtered by MIA-Sig (available in github), and then generated tab-delimited txt file.
DNA-binding protein coverage files (bedgraph) were generated by bedtools (version 2.26.0) with genomecov command. Coverage files were sorted by chromosome and coordinate.
ChIA-PET loop files (bedpe) were generated by our in-house ChIA-PET processing pipeline, available in github (ChIA-PIPE).
Peaks (broadPeak, bed file type) were called by SPP (version 1.13) with our input control file (sample# 63, library ID: CHG0033).
2D contact map files (hic) were generated by Juicer Tools (Version 1.7.5) using chromatin interaction data from ChIA-PET and ChIA-Drop. In ChIA-Drop, we used all pairwise interactions from each GEM.
Genome_build: hg38
Supplementary_files_format_and_content: tab-delimited txt format of ChIA-Drop, containing GEM_ID, GEM_coordinate, GEM_span, Fragment_number, List_of_fragment_coordinates. Each row represents one GEM.
Supplementary_files_format_and_content: bedgraph format of ChIA-Drop, ChIP-seq, and ChIA-PET, containing a DNA-binding protein coverage
Supplementary_files_format_and_content: bedpe format of ChIA-PET, containing ChIA-PET loop (long-range interaction data) data
Supplementary_files_format_and_content: broadPeak format of ChIP-seq and ChIA-PET, containing peaks called by SPP software
Supplementary_files_format_and_content: hic format of ChIA-Drop and ChIA-PET, 2D contact map file, visualized by Juicebox software

value: RNAPII-ChIA-PET

value: RNAPII (BioLegend, catalog# 664912)


title: sn_p1_smrt2_R26

description: 1 single nucleus

data_processing:
NextSeq 550 RTA software
data were processed with nf-core/rnaseq (https://github.com/nf-core/rnaseq)
deviation from default pipeline options (star_salmon): parameters for trimming samples 191-210: --clip_r1 19 --clip_r2 9 --three_prime_clip_r1 5 --three_prime_clip_r2 5
counts table was analysed with Deseq2 and p-Values calculated with Wald-test
Genome_build: Tair10
Supplementary_files_format_and_content: Matrix table with raw counts for every gene and transposon for every sample

source_name: seedling

growth_protocol: seedlings were grown on soil at standard growth conditions (16h light/8h dark) and shoot apices harvested 7 days after germination.

extract_protocol:
nuclei were extracted according to (DOI: 10.1007/978-1-0716-0179-2_7) and either 50 (bulk), 0 (neg control) or 1 single nucleus sorted into a single well.
For samples 1-190: Smart-seq2 single cell protocol (DOI: 10.1038/nmeth.2639). For samples 191-210: Smart-seq3 single cell protocol (according to Hagemann-Jensen et. al, https://doi.org/10.1038/s41587-020-0497-0).

value: 7 days after germination

value: stem cells


title: Sample 62_wt_sperm_R2


title: GM19239-NE-ChIA-Drop-Rep5

description:
Sample 57
nuclear chromatin
ChIA-Drop
GM19239-NE-pooled.txt
GM19239-NE-pooled.hic


title: H2O2_saline_3

source_name: H2O2_saline-treated mice

value: intravesical H2O2 infusion and intraperitoneal injection of saline


title: GM19239-NE-ChIA-Drop-Rep4

description:
Sample 56
nuclear chromatin
ChIA-Drop
GM19239-NE-pooled.txt
GM19239-NE-pooled.hic

value: ChIA-Drop


title: Sample 61_wt_sperm_R1


title: L929sAhFas, Ferroptosis X04-ML162, E375

city: Gosselies

first: Denis

middle: LJ

last: Lafontaine

state: Hainaut

address: Av Profs Jeneer & Brachet, 12

department: RNA Molecular Biology

country: Belgium

institute: Universite libre de Bruxelles

data_processing:
Using Linux/ubuntu
Sequence reads were trimmed for adaptor sequence/low-quality sequence using trimmomatics 0.39 with the following parameters : LEADING:30 TRAILING:30 SLIDINGWINDOW:4:15 AVGQUAL:30 MINLEN:17 TOPHRED33
Reads are alligned to refrence sequences using bowtie2
refrence sequences: rRNA 28S: NR_003279.1; rRNA 5.8S: NR_003280.2; rRNA 18S: NR_003278.3
Aligned reads are sorted, converted to bam files and indexed using samtools
Methylation score is calculated in r using the code from Felix Ernst (https://github.com/FelixErnst/RNAmodR.RiboMethSeq)
Assembly: GRCm38.p6
Supplementary files format and content: Tab-delimited text file containening the MethScore of all samples
Library strategy: RiboMethSeq

extract_protocol:
Total RNA was extracted from cells or purified EVs using the TRI reagent (ThermoFisher, ARNA was extracted from purified EVs using the TRI reagent (ThermoFisher, AM9738), according to the manufacturer’s instructions and hydrolyzed in bicarbonate buffer. The reaction was stopped by ethanol precipitation.
The resulting fragments were then end-repaired and converted into an Illumina library using the NEBNext Small RNA Library Prep kit (Bioke, E7300S) and sequenced at the ULB brightcore facility.

value: murine fibrosarcoma cells

value: ML162-induced ferroptosis


title: L929sAhFas, Ferroptosis X04-ML162, E373


title: L929sAhFas, Apoptosis X03-aFas, E375

value: hFasL-induced apoptosis


title: H2O2_saline_2


title: Sample 60_ago9_vn_R4


title: GM19239-NE-ChIA-Drop-Rep3

description:
Sample 55
nuclear chromatin
ChIA-Drop
GM19239-NE-pooled.txt
GM19239-NE-pooled.hic

value: HindIII fragmented chromatin, including all chromatin associated proteins


title: Sample 59_ago9_vn_R3


title: L929sAhFas, Apoptosis X03-aFas, E373


title: control_3

source_name: control mice

value: wild-type

value: untreated


title: GM19239-NE-ChIA-Drop-Rep2

description:
Sample 54
nuclear chromatin
ChIA-Drop
GM19239-NE-pooled.txt
GM19239-NE-pooled.hic


title: L929sAhFas, Necroptosis X02-TNF, E375

value: TNF-induced necroptosis


title: H2O2_saline_1

value: total bladder


title: control_2


title: Sample 58_ago9_vn_R2

value: 35 days after germination


title: control_1


title: Sample 57_ago9_vn_R1

value: vegetative cells


title: GM12878-RNAPII-ChIA-Drop-Rep14

description:
Sample 51
nuclear chromatin
RNAPII-ChIA-Drop
GM12878-RNAPII-pooledv2_comp.txt
GM12878-RNAPII-pooledv2.bedgraph
GM12878-RNAPII-pooledv2.hic

value: RNAPII-ChIA-Drop


title: Sample 56_ago9_sperm_R3

value: sperm cells


title: GM19239-NE-ChIA-Drop-Rep1

description:
Sample 53
nuclear chromatin
ChIA-Drop
GM19239-NE-pooled.txt
GM19239-NE-pooled.hic


title: L929sAhFas, Untreated X01-UT, E375

value: steady state growth


title: L929sAhFas, Untreated X01-UT, E373 2


title: L929sAhFas, Necroptosis X02-TNF, E373


title: GM12878-RNAPII-ChIA-Drop-Rep15

description:
Sample 52
nuclear chromatin
RNAPII-ChIA-Drop
GM12878-RNAPII-pooledv2_comp.txt
GM12878-RNAPII-pooledv2.bedgraph
GM12878-RNAPII-pooledv2.hic

value: RNAPII (BioLegend, catalog# 664912)


title: Sample 55_ago9_sperm_R2


title: GM12878-RNAPII-ChIA-Drop-Rep12

description:
Sample 49
nuclear chromatin
RNAPII-ChIA-Drop
GM12878-RNAPII-pooledv2_comp.txt
GM12878-RNAPII-pooledv2.bedgraph
GM12878-RNAPII-pooledv2.hic


title: L929sAhFas, Untreated X01-UT, E373


title: GM12878-RNAPII-ChIA-Drop-Rep11

description:
Sample 48
nuclear chromatin
RNAPII-ChIA-Drop
GM12878-RNAPII-pooledv2_comp.txt
GM12878-RNAPII-pooledv2.bedgraph
GM12878-RNAPII-pooledv2.hic


title: GM12878-RNAPII-ChIA-Drop-Rep13

description:
Sample 50
nuclear chromatin
RNAPII-ChIA-Drop
GM12878-RNAPII-pooledv2_comp.txt
GM12878-RNAPII-pooledv2.bedgraph
GM12878-RNAPII-pooledv2.hic


title: Sample 52_ago5_vn_R2


title: GM12878-RNAPII-ChIA-Drop-Rep10

description:
Sample 47
nuclear chromatin
RNAPII-ChIA-Drop
GM12878-RNAPII-pooledv2_comp.txt
GM12878-RNAPII-pooledv2.bedgraph
GM12878-RNAPII-pooledv2.hic


title: GM12878-SMC1A-ChIA-Drop-Rep6

description:
Sample 37
nuclear chromatin
SMC1A-ChIA-Drop
GM12878-cohesin-pooled_comp.txt
GM12878-cohesin-pooled.bedgraph
GM12878-cohesin-pooled.hic

value: SMC1 (Bethyl Laboratories, catalog# A300-055A)


title: Lesions from Chi3l1 knockout MIC, Chil12wk6405

city: Montreal

first: Sherif

middle: Samer

last: Attalla

state: Quebec

address: 1160 Ave. Pine W

department: Goodman Cancer Institute

country: Canada

institute: McGill

description:
DEG_2
FPKM_all_samples.txt

data_processing:
Adaptor sequences and low quality score bases (Phred score < 30) were trimmed using Trimmomatic (Bolger et al., 2014)
Reads were aligned to the GRCm38 mouse reference genome assembly using STAR (Dobin et al., 2012)
Read counts were obtained using HTSeq (Anders et al., 2015) with parameters -m intersection-nonempty -stranded=no
Raw counts were normalized using edgeR’s TMM algorithm (Robinson et al., 2010) and were then transformed to log2-counts per million (logCPM) using the voom function implemented in the limma R package (Ritchie et al., 2015)
To assess differences in gene expression levels between the different conditions, we fitted a linear model using limma’slmfit function(method="robust" parameter was applied for end-stage tumor dataset)
Nominal p-values were corrected for multiple testing using the Benjamini-Hochberg method
Distinct patterns of gene expression changes were identified using unsupervised hierarchical clustering method (R function hclust with Euclidean distance and complete linkage metrics)
Assembly: GrCM38/mm10
Supplementary files format and content: A tab-delimited text file includes FPKM values for all samples.

source_name: Hyperpalstic Mammary gland

extract_protocol:
Qiagen RNEasy Mini kit was used according to the manufacturer's instructions
RNA libraries for RNA-seq were prepared using the NEBNext Ultra II Library Prep Kit following manufacturer's protocols.

tag: strain

value: FVB/N

tag: Sex

value: Female

value: Hyperpalstic Mammary gland

value: MIC Chi3l1 ko

value: Doxycyline (2mg/ml)


title: Lesions from Chi3l1 knockout MIC, Chil12wk6389

description:
DEG_2
FPKM_all_samples.txt


title: Lesions from Chi3l1 knockout MIC, Chil12wk6409

description:
DEG_2
FPKM_all_samples.txt


title: Lesions from Chi3l1 knockout MIC, Chil12wk6377

description:
DEG_2
FPKM_all_samples.txt


title: Sample 50_ago5_sperm_R3

tag: ecotype


title: Lesions from Stat3 knockout MIC, Stat32wk1925

description:
DEG_1
FPKM_all_samples.txt

tag: tissue

value: MIC Stat3 ko


title: Sample 49_ago5_sperm_R2

tag: cell type


title: GM12878-SMC1A-ChIA-Drop-Rep4

description:
Sample 35
nuclear chromatin
SMC1A-ChIA-Drop
GM12878-cohesin-pooled_comp.txt
GM12878-cohesin-pooled.bedgraph
GM12878-cohesin-pooled.hic


title: Thalami of saline injected Maged1 cKO mice-RNAseq_ replicate 4

city: Lyon

first: Leonardo

last: Beccari

address: 8 Avenue Rockefeller

country: France

institute: Institute NeuroMyoGene (PGNM)

description: stranded ribodepleted  RNAseq

data_processing:
Approximately 20 millions of paired-end reads per sample were mapped against the mouse reference genome (GRCm38.p4/mm10) using STAR 2.5.3a software (13) to generate read alignments for each sample. Annotations Mus_musculus.GRCm38.87.gtf were obtained from ftp.Ensembl.org. Read counts and transcript per million reads (TPMs) were generated using tximport R package version 1.10.0 and lengthScaledTPM method (Soneson et al., 2016) with inputs of transcript quantifications from tool salmon (Patro et al., 2017). Low expressed transcripts and genes were filtered based on analysing the data mean-variance trend. The TMM method was used to normalise the gene and transcript read counts to log_2-CPM (Bullard et al., 2010).
noen provided by the submitter

source_name: Thalamus

growth_protocol: Conditional knockout mice were generated by crossing heterozygous Maged1 loxp/WT or homozygous Maged1 loxp/loxp females with different population selective Cre male mice. The Cre-mediated deletion of Maged1 was obtained using mice expressing Cre-recombinase under the control of the vGluT2 promoter. Maged1 WT or Maged1 cKO adult animals (2-4 months ) were used for RNAseq experiments.

extract_protocol:
Total mRNA from six sorted independent samples (3 vGluT2 Cre, loxp-stop-loxp-tdTomato, Maged1 loxp and 3 vGluT2 Cre, loxp-stop-loxp-tdTomato, Maged1 WT) and from 20 unsorted independent samples (vGluT2 Cre::Maged1 loxp or WT, 9 with chronic cocaine, 11 with saline inejctions) was extracted using the QIAGEN miRNeasy micro kit according to the manufacturer’s recommendations.
RNA quality was assessed on a Bioanalyzer 2100 (Agilent technologies). After RNA extraction, ribosomial RNA was rmoved from samples using the NEBNext rRNA Depletion Kit (New England BioLabs). Next, barcoded libraries were NEBNext Ultra II Directional RNA Library Prep kit (New England BioLabs), following manufacturer’s recommendations. The multiplexed libraries (18 pM) were loaded on flow cells and sequenced by paired-end 100bp reads in a Novaseq 6000 system (Illumina) to obtain a sequencing depth of at least  20 million reads per sample.
stranded RNAseq (ribodepletion)

treatment_protocol: Maged1 WT or Maged1 cKO mice were injected for three days with saline solution, followed by 5 days of cocaine or saline vehicle administration (20 mg/kg, intraperitoneal).

value: Thalamus

value: neural tissue

value: 2-4 months old mice

value: saline


title: Sample 54_ago9_sperm_R1

value: 35 days after germination


title: Sample 53_ago5_vn_R4

value: vegetative cells


title: Thalami of saline injected Maged1 cKO mice-RNAseq_ replicate 3

description: stranded ribodepleted  RNAseq


title: Thalami of saline injected Maged1 cKO mice-RNAseq_ replicate 2

description: stranded ribodepleted  RNAseq

value: Maged1 Loxp


title: Sample 47_ago59_vn_R4


title: Lesions from Stat3 knockout MIC, Stat32wk2527

description:
DEG_1
FPKM_all_samples.txt


title: GM12878-SMC1A-ChIA-Drop-Rep2

description:
Sample 33
nuclear chromatin
SMC1A-ChIA-Drop
GM12878-cohesin-pooled_comp.txt
GM12878-cohesin-pooled.bedgraph
GM12878-cohesin-pooled.hic


title: Lesions from Stat3 knockout MIC, Stat32wk4634

description:
DEG_1
FPKM_all_samples.txt


title: Thalami of saline injected Maged1 cKO mice-RNAseq_ replicate 1

description: stranded ribodepleted  RNAseq


title: Thalami of cocaine injected Maged1 cKO mice-RNAseq_ replicate 3

description: stranded ribodepleted  RNAseq

value: cocaine


title: Sample 51_ago5_vn_R1


title: GM12878-RAD21-ChIA-Drop-Rep13

description:
Sample 31
nuclear chromatin
RAD21-ChIA-Drop
GM12878-cohesin-pooled_comp.txt
GM12878-cohesin-pooled.bedgraph
GM12878-cohesin-pooled.hic

value: RAD21 (Abcam, catalog# ab922)


title: Sample 46_ago59_vn_R3


title: Lesions from WT MIC, WT2wk2040

description:
DEG_1
FPKM_all_samples.txt

value: MIC WT


title: Lesions from WT MIC, WT2wk2041

description:
DEG_1
FPKM_all_samples.txt


title: GM12878-SMC1A-ChIA-Drop-Rep5

description:
Sample 36
nuclear chromatin
SMC1A-ChIA-Drop
GM12878-cohesin-pooled_comp.txt
GM12878-cohesin-pooled.bedgraph
GM12878-cohesin-pooled.hic

value: SMC1 (Bethyl Laboratories, catalog# A300-055A)


title: GM12878-SMC1A-ChIA-Drop-Rep3

description:
Sample 34
nuclear chromatin
SMC1A-ChIA-Drop
GM12878-cohesin-pooled_comp.txt
GM12878-cohesin-pooled.bedgraph
GM12878-cohesin-pooled.hic


title: Sample 44_ago59_vn_R1


title: Sample 48_ago5_sperm_R1

value: sperm cells


title: Sample 43_ago59_sperm_R3


title: GM12878-SMC1A-ChIA-Drop-Rep1

description:
Sample 32
nuclear chromatin
SMC1A-ChIA-Drop
GM12878-cohesin-pooled_comp.txt
GM12878-cohesin-pooled.bedgraph
GM12878-cohesin-pooled.hic


title: GM12878-RAD21-ChIA-Drop-Rep11

description:
Sample 29
nuclear chromatin
RAD21-ChIA-Drop
GM12878-cohesin-pooled_comp.txt
GM12878-cohesin-pooled.bedgraph
GM12878-cohesin-pooled.hic


title: Lesions from WT MIC, WT2wk2043

description:
DEG_1
FPKM_all_samples.txt

value: FVB/N

value: Female


title: GM12878-RAD21-ChIA-Drop-Rep10

description:
Sample 28
nuclear chromatin
RAD21-ChIA-Drop
GM12878-cohesin-pooled_comp.txt
GM12878-cohesin-pooled.bedgraph
GM12878-cohesin-pooled.hic


title: Sample 42_ago59_sperm_R2


title: Thalami of saline injected Maged1 WT mice-RNAseq_ replicate 4

description: stranded ribodepleted  RNAseq


title: Sample 45_ago59_vn_R2


title: Thalami of saline injected Maged1 WT mice-RNAseq_ replicate 3

description: stranded ribodepleted  RNAseq


title: GM12878-RAD21-ChIA-Drop-Rep9

description:
Sample 27
nuclear chromatin
RAD21-ChIA-Drop
GM12878-cohesin-pooled_comp.txt
GM12878-cohesin-pooled.bedgraph
GM12878-cohesin-pooled.hic


title: GM12878-RAD21-ChIA-Drop-Rep8

description:
Sample 26
nuclear chromatin
RAD21-ChIA-Drop
GM12878-cohesin-pooled_comp.txt
GM12878-cohesin-pooled.bedgraph
GM12878-cohesin-pooled.hic


title: L929sAhFas, Apoptosis_X03-aFas, E375

data_processing:
Using Linux/ubuntu
Sequence reads were trimmed for adaptor sequence/low-quality sequence using trimmomatics 0.39 with the following parameters : LEADING:30 TRAILING:30 SLIDINGWINDOW:4:15 AVGQUAL:30 MINLEN:17 TOPHRED33
Reads are alligned to refrence sequences using bowtie2
refrence sequences: rRNA 28S: NR_003279.1; rRNA 5.8S: NR_003280.2; rRNA 18S: NR_003278.3
Aligned reads are sorted, converted to bam files and indexed using samtools
Methylation score is calculated in r using the code from Felix Ernst (https://github.com/FelixErnst/RNAmodR.RiboMethSeq)
Assembly: GRCm38.p6
Supplementary files format and content: Tab-delimited text file containening the MethScore of all samples

source_name: EV extracted RNA

extract_protocol:
Total RNA was extracted from purified EVs using the TRI reagent (ThermoFisher, AM9738), according to the manufacturer’s instructions and hydrolyzed in bicarbonate buffer. The reaction was stopped by ethanol precipitation.
The resulting fragments were then end-repaired and converted into an Illumina library using the NEBNext Small RNA Library Prep kit (Bioke, E7300S) and sequenced at the ULB brightcore facility.
RiboMethSeq (DOI: 10.1093/nar/gkw547)

value: murine fibrosarcoma cells

value: hFasL-induced apoptosis


title: L929sAhFas, Necroptosis_X02-TNF, E375

value: TNF-induced necroptosis


title: GM12878-RAD21-ChIA-Drop-Rep7

description:
Sample 25
nuclear chromatin
RAD21-ChIA-Drop
GM12878-cohesin-pooled_comp.txt
GM12878-cohesin-pooled.bedgraph
GM12878-cohesin-pooled.hic


title: L929sAhFas, Ferroptosis_X04-ML162, E373

value: ML162-induced ferroptosis


title: GM12878-RAD21-ChIA-Drop-Rep12

description:
Sample 30
nuclear chromatin
RAD21-ChIA-Drop
GM12878-cohesin-pooled_comp.txt
GM12878-cohesin-pooled.bedgraph
GM12878-cohesin-pooled.hic


title: Lesions from WT MIC, WT2wk2042

description:
DEG_1
FPKM_all_samples.txt

value: Hyperpalstic Mammary gland

value: Doxycyline (2mg/ml)


title: Thalami of cocaine injected Maged1 cKO mice-RNAseq_ replicate 2

description: stranded ribodepleted  RNAseq

value: Thalamus

value: neural tissue

value: 2-4 months old mice


title: Thalami of cocaine injected Maged1 cKO mice-RNAseq_ replicate 1

description: stranded ribodepleted  RNAseq


title: Thalami of cocaine injected Maged1 WT mice-RNAseq_ replicate 4

description: stranded ribodepleted  RNAseq

value: Maged1 Loxp


title: Sample 22_ago9_stem_R2

description: resequencing of sample 14

data_processing:
Base calling: NextSeq 550 RTA software
data were processed with nf-core/methylseq (https://github.com/rbpisupati/methylseq). Reads were trimmed using Trim Galore! and aligned with Bismark. Parameters used for zymo-kit: --zymo True
for samples 9-16: to increase mapping efficiency according to (https://sequencing.qcfail.com/articles/pbat-libraries-may-generate-chimaeric-read-pairs/): Reads first mapped paired-end (--pbat) and unmapped reads written out. Unmapped R1 next mapped in single-end mode (--pbat). Unmapped R2 mapped in single-end mode (directional). Files are merged in methylpy to generate allc files (see next step).
allc files are generated with methylpy (https://pypi.org/project/methylpy/) for CG, CHG,CHH context and for each genotype/cell type merged using merge-allc. Weighted methylation levels for features are calculated using BEDTools intersect (bedtools v2.30.0) and R from number of reads supporting methylation and read coverage.
Genome_build: Tair10
Supplementary_files_format_and_content: Matrix table for all samples containing: number of methylated C, total number of C, methylation levels for genomic features (genes and transposons), in CG, CHG and CHH context

growth_protocol: For samples 1-8: Plants were grown on soil in standard growth conditions (16h light/8h dark) pollen was harvested accoding to (DOI: 10.1111/j.1365-313X.2004.02147.x); for samples 9-24: seedlings were grown on soil in standard growth conditions (16h light/8h dark) and harvested 7 days after germination.

extract_protocol:
For samples 1-8: nuclei were extracted and sorted according to (DOI: 10.1007/s00497-015-0258-2); per replicate 50,000 nuclei were sorted into DNA lysis buffer (Zymo research #D3021) and extracted using the Quick-DNA Microprep Kit (Zymo research #D3021). For samples 9-24: nuclei were extracted and sorted for mCherry according to (DOI: 10.1007/978-1-0716-0179-2_7); per replicate 3000-4000 nuclei were sorted into DNA lysis buffer (Zymo research #D3021) and extracted using the Quick-DNA Microprep Kit (Zymo research #D3021).
1-5ng of genomic DNA was used and libraries were construced with the Pico Methyl-Seq Library Prep Kit (Zymo research #D5456)

value: 7 days after germination

value: stem cells


title: L929sAhFas, Apoptosis_X03-aFas, E373


title: GM12878-RAD21-ChIA-Drop-Rep6

description:
Sample 24
nuclear chromatin
RAD21-ChIA-Drop
GM12878-cohesin-pooled_comp.txt
GM12878-cohesin-pooled.bedgraph
GM12878-cohesin-pooled.hic

value: RAD21 (Abcam, catalog# ab922)


title: Thalami of cocaine injected Maged1 WT mice-RNAseq_ replicate 3

description: stranded ribodepleted  RNAseq

value: cocaine


title: L929sAhFas, Ferroptosis_X04-ML162, E375


title: Sample 21_ago9_stem_R1

description: resequencing of sample 13


title: GM12878-RAD21-ChIA-Drop-Rep5

description:
Sample 23
nuclear chromatin
RAD21-ChIA-Drop
GM12878-cohesin-pooled_comp.txt
GM12878-cohesin-pooled.bedgraph
GM12878-cohesin-pooled.hic


title: Sample 20_ago5_stem_R2

description: resequencing of sample 12


title: Thalami of cocaine injected Maged1 WT mice-RNAseq_ replicate 2

description: stranded ribodepleted  RNAseq


title: L929sAhFas, Necroptosis_X02-TNF, E373


title: Sample 41_ago59_sperm_R1

value: 35 days after germination

value: sperm cells


title: L929sAhFas, Untreated_X01-UT, E373

value: steady state growth


title: Sample 23_ago59_stem_R1

description: resequencing of sample 15


title: GM12878-RAD21-ChIA-Drop-Rep4

description:
Sample 22
nuclear chromatin
RAD21-ChIA-Drop
GM12878-cohesin-pooled_comp.txt
GM12878-cohesin-pooled.bedgraph
GM12878-cohesin-pooled.hic


title: Sample 24_ago59_stem_R2

description: resequencing of sample 16


title: Thalami of saline injected Maged1 WT mice-RNAseq_ replicate 1

description: stranded ribodepleted  RNAseq

value: saline


title: Thalami of saline injected Maged1 WT mice-RNAseq_ replicate 2

description: stranded ribodepleted  RNAseq


title: Sample 19_ago5_stem_R1

description: resequencing of sample 11


title: GM12878-RAD21-ChIA-Drop-Rep3

description:
Sample 21
nuclear chromatin
RAD21-ChIA-Drop
GM12878-cohesin-pooled_comp.txt
GM12878-cohesin-pooled.bedgraph
GM12878-cohesin-pooled.hic


title: Sample 17_wt21_stem_R1

description: resequencing of sample 9


title: Lesions from Stat3 knockout MIC, Stat32wk2381

description:
DEG_1
FPKM_all_samples.txt

value: FVB/N

value: Female

value: MIC Stat3 ko


title: Control scTCR

city: Chicago

first: Jun

last: Huang

state: IL

address: Eckhardt, 5640 S Ellis Ave

department: Institute for Molecular Engineering

institute: University of Chicago

description: TCR mRNA

data_processing:
The scRNA-seq reads were aligned to the mm10 reference genome and quantified using the cellranger count (10X Genomics, version 3.0.0). The filtered gene-barcode matrices that contained only barcodes with unique molecular identifier (UMI) counts that passed the quality control were used for downstream analyses. We obtained a total of 20,062 cells from 3 samples with on average 65,315 reads per cell and a median of 1,579 genes per cell.
The scTCR-seq reads were aligned to the 10x curated mm10 vdj reference genome and quantified using the cellranger vdj (10X Genomics, version 3.0.0). The resultant clonotype and filtered contig annotation data were used for downstream analyses. The clonotype and contig data were then added to the scRNA data by matching the cell barcodes. The frequency of each clonotype in each sample were recalculated based on those successfully matched to the corresponding scRNA data. An overall frequency of each clonotype was also calculated by pooling all clonotypes from the Naïve, Control and Vaccine data and then recalculating. The above analyses were done for CD4 and CD8 separately.
Genome_build: mm10
Supplementary_files_format_and_content: filtered expression matrix for scRNA
Supplementary_files_format_and_content: filtered clonotype and contigs for scTCR

source_name: CD4+ and CD8+ T cells

extract_protocol:
Tumors were removed and cut into small pieces (~2 mm) with scissors. Tumor pieces were incubated in a digestion buffer containing 1 mg/ml collagenase type IV (Millipore Sigma, Darmstadt, Germany), in RPMI 1640 for 45 minutes (min) in a shaking incubator (Eppendorf New Brunswick Excella E25) at 37 °C and 200 RPM. Digested cell suspensions were passed through 100 μm mesh filters and were enriched for immune cells using Percoll (GE Healthcare Life Sciences, Pittsburgh, Pennsylvania, USA) density gradient centrifugation—— resuspended in 40% Percoll, layered on top of 60% Percoll and centrifuged for 30 min at 900x g at room temperature, without brake. Cells at the interface of the two Percoll layers were harvested and subsequently washed with FACS buffer in preparation for antibody staining.
Cellular suspensions were loaded on a 10X Genomics GemCode Single-cell instrument that generates single-cell Gel Bead-In-EMlusion (GEMs) with Chromium Next GEM Single Cell 5’ Reagent Kits v2（PN-1000263.The expression libraries were generated from the half of cDNAs with Library Construction Kit (PN-1000190) and the TCR libraries were constructed with the other half cDNAs by Chromium Single Cell Mouse TCR Amplification Kit(PN-1000254).Both of the libraries were sequenced using Illumina Novaseq6000 by Gene Denovo Biotechnology Co.(Guangzhou, China).
Single-cell RNA-seq
Single-cell TCR-seq

value: Tumor

tag: disease state


title: GM12878-RAD21-ChIA-Drop-Rep2

description:
Sample 20
nuclear chromatin
RAD21-ChIA-Drop
GM12878-cohesin-pooled_comp.txt
GM12878-cohesin-pooled.bedgraph
GM12878-cohesin-pooled.hic


title: Mettl4 knockout NSCs, rep1

city: Oslo

first: Xu

last: Zhao

address: Sognsvannsveien 20

department: Department of Microbiology

country: Norway

institute: Oslo University Hospital, Rikshospitalet

data_processing:
Raw data were quality controlled and trimmed of adapter sequences by using Fastp (v0.23.2).
The clear reads were mapped and annotated to the mouse genome with STAR (v2.7.10) using 2-PASS method.
For RNA expression analysis, featureCounts embedded in R package Rsubread (v3.15) was used for counting reads.
Assembly: Gencode.vM28.GRCm39
Supplementary files format and content: tab-delimited text files include raw counts for each RNA sample

source_name: brain

growth_protocol: The cortex extracted from E14.5 embryo was dissected in PBS, followed by the incubation with 0.05% trypsin and 200 μM EDTA in PBS at 37°C for 5 min. Then the tissue was mechanically dissociated using a fire-polished Pasteur pipette and filtrated using a 40 μm Cell Strainer (BD Falcon, NJ). Then the cells were seeded and amplified in non-adherent T75 flasks and incubated in a humidified atmosphere of 5% CO2 and 95% air at 37°C. The complete medium consisted of DMEM/F12 (1:1), 20 ng/ml EGF (R&D Systems, 236-EG-200), 10 ng/ml bFGF (R&D Systems, 234-FSE 025), 1% of N2 supplement (Life, 17,502–048), and 1% of B27 supplement without vitamin A (Thermo, 12,587,010). After 5 to 7 days, cells propagated in primary neurospheres (P0 cells) with the diameter of approximately 80-200 μm were dissociated into single cells. These cells were cultured in suspension and allowed to form the secondary neurospheres (P1 cells). To obtain a uniform population of NSCs, P1 cells were further dissociated and the single cells were plated onto poly-D-lysine (PDL)–coated (100 μg/ml) plates or glass coverslips and grown as a monolayer on the adherent surface.

extract_protocol:
The cells were washed three times with cold PBS. Then they were collected in TRIzol (Invitrogen, 15596026) and sent to Novogene Co., Ltd. (Beijing, China) for RNA extraction, library construction and RNA sequencing.
RNA library was constructed by using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® according to manufacture's protocol.

value: brain

value: primary neural stem cells

value: Mettl4 knockout


title: GM12878-RAD21-ChIA-Drop-Rep1

description:
Sample 19
nuclear chromatin
RAD21-ChIA-Drop
GM12878-cohesin-pooled_comp.txt
GM12878-cohesin-pooled.bedgraph
GM12878-cohesin-pooled.hic


title: wild type NSCs, rep 2

value: wild type


title: NaïveLN scTCR

description: TCR mRNA

value: Lymph node

value: Naïve (without tumor)


title: GM12878-CTCF-ChIA-Drop-Rep17

description:
Sample 17
nuclear chromatin
CTCF-ChIA-Drop
GM12878-CTCF-pooled_comp.txt
GM12878-CTCF-pooled.bedgraph
GM12878-CTCF-pooled.hic

value: CTCF-ChIA-Drop

value: CTCF (Abclonal, catalog# A1133)


title: Sample 16_ago59_stem_R2

value: 7 days after germination

value: stem cells


title: Vaccine scTCR

description: TCR mRNA

value: Tumorumor


title: wild type NSCs, rep 1


title: Vaccine scRNA

description: mRNA


title: Sample 15_ago59_stem_R1


title: GM12878-CTCF-ChIA-Drop-Rep10

description:
Sample 10
nuclear chromatin
CTCF-ChIA-Drop
GM12878-CTCF-pooled_comp.txt
GM12878-CTCF-pooled.bedgraph
GM12878-CTCF-pooled.hic


title: GM12878-CTCF-ChIA-Drop-Rep9

description:
Sample 9
nuclear chromatin
CTCF-ChIA-Drop
GM12878-CTCF-pooled_comp.txt
GM12878-CTCF-pooled.bedgraph
GM12878-CTCF-pooled.hic


title: Thalami of cocaine injected Maged1 WT mice-RNAseq_ replicate 1

description: stranded ribodepleted  RNAseq

value: Thalamus

value: neural tissue

value: 2-4 months old mice

value: Maged1 Loxp

value: cocaine


title: Control scRNA

description: mRNA

value: Tumor


title: Sample 14_ago9_stem_R2


title: Wildtype mESCs H3K4me3

city: Macau

first: Gang

last: Li

address: E12 Avenida da Universidade, Taipa, Macau

department: Faculty of Health Sciences

country: Macau

institute: University of Macau

data_processing:
Illumina RTA software was used for basecalling and Illumina bcl2fastq 2.17 software was used for demultiplexing.
Sequencing reads were quality checked with FastQC v0.11.7.
Reads were trimmed with TrimGalore v0.6.0 to remove low quality ends, adaptor sequence (--stringency5) and reads shorter than 50 bps (for paired end reads) or 35 bps (for single end reads).
The pre-processed reads were aligned to the mm10 mouse reference genome using  Bowtie2 v2.3.4.1.
The aligned reads with mapQ > 10 were converted to bam format using Samtools v1.9. Duplicated reads were removed using Picard v2.18.5 - MarkDuplicates, keeping reads with mapQ > 10 that are not within the ENCODE DAC blacklist regions.
Genome_build: mm10 (GRCm38)
Supplementary_files_format_and_content: bigWig files were generated using Deeptools v3.0.2 with RPKM (reads per kilobase per million) normalization.

source_name: mouse embryonic stem cells

growth_protocol: Wildtype mESCs, EED knockout mutant mESCs, FLAG-EED wildtype and FLAG-EED K19R mutant knock-in mESCs were cultured on gelatin-coated dishes in high-glucose DMEM supplemented with 15% FBS, 1mM sodium pyruvate (Gibco), 1x GlutaMAX (Gibco), 1x nonessential amino acids (NEAA, Gibco), 40 μM β-mercaptoethanol (Acros Organics), 1000 U/ml Leukemia Inhibitory Factor (LIF) (EMD Millipore), 1% Penicillin/Streptomycin (Gibco), with or without 1 μM MEK1/2 inhibitor (PD0325901) and 3 μM GSK3 inhibitor (CHIR99021) at 37°C in a humidified atmosphere with 5% (v/v) CO2.

extract_protocol:
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody.
Libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (E7645L).

treatment_protocol: Wildtype mESCs and EED knockout mutant mESCs were culture with LIF. FLAG-EED wildtype and FLAG-EED K19R mutant knock-in mESCs were cultured with LIF + 2i and treated with TSA (2 uM or 100 ng/l) for indicated times.

value: embryonic stem cells

value: wildtype

value: culture with LIF

tag: chip antibody

value: H3K4me3 (Millipore, 07-473)


title: Wildtype mESCs TSA 12h H3K4me3

tag: genotype

value: culture with LIF, treated with TSA for 12h


title: Sample 13_ago9_stem_R1


title: NaïveLN scRNA

description: mRNA

value: Lymph node

value: Naïve (without tumor)


title: Wildtype mESCs TSA 12h EZH2


title: Sample 18_wt21_stem_R2

description: resequencing of sample 10


title: Mettl4 knockout NSCs, rep2

value: brain

value: primary neural stem cells

value: Mettl4 knockout


title: GM12878-CTCF-ChIA-Drop-Rep8

description:
Sample 8
nuclear chromatin
CTCF-ChIA-Drop
GM12878-CTCF-pooled_comp.txt
GM12878-CTCF-pooled.bedgraph
GM12878-CTCF-pooled.hic

value: CTCF-ChIA-Drop

value: CTCF (Abclonal, catalog# A1133)


title: GM12878-CTCF-ChIA-Drop-Rep7

description:
Sample 7
nuclear chromatin
CTCF-ChIA-Drop
GM12878-CTCF-pooled_comp.txt
GM12878-CTCF-pooled.bedgraph
GM12878-CTCF-pooled.hic


title: Sample 12_ago5_stem_R2

value: 7 days after germination

value: stem cells


title: Wildtype mESCs TSA 12h H3K27me3

value: H3K27me3 (Abcam, ab6002)


title: Sample 11_ago5_stem_R1


title: Wildtype mESCs TSA 12h EED-K19ac

value: EED-K19ac (In house)


title: Sample 10_wt21_stem_R2


title: GM12878-CTCF-ChIA-Drop-Rep3

description:
Sample 3
nuclear chromatin
CTCF-ChIA-Drop
GM12878-CTCF-pooled_comp.txt
GM12878-CTCF-pooled.bedgraph
GM12878-CTCF-pooled.hic


title: GM12878-CTCF-ChIA-Drop-Rep4

description:
Sample 4
nuclear chromatin
CTCF-ChIA-Drop
GM12878-CTCF-pooled_comp.txt
GM12878-CTCF-pooled.bedgraph
GM12878-CTCF-pooled.hic


title: Sample 9_wt21_stem_R1


title: Wildtype mESCs TSA 2h H3K4me3

value: culture with LIF, treated with TSA for 2h


title: The HaCaT cell lines, overexpression of vectors of GJB2 gene mutant  p.Asp66His(D66H) 2

type: RNA

city: Jinan

first: Li

last: Zhang

address: Department of Dermatology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250021, China.

country: China

hyb_protocol: Following fragmentation, cRNA were hybridized for 16-18 hr at 45 ℃ on GeneChip PrimeView Human Gene Expression Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450

scan_protocol: GeneChips were scanned using the GeneChip Scanner 300

data_processing:
The gene expression profile was preprocessed using Limma package in Bioconductor and Affymetrix annotation files. The background correction, quantile normalization and probe summarization of the microarray data were performed using the Robust Multiarray average algorithm to obtain the gene expression matrix. The cut-off for the background correction was 20%, and the coefficient of variation was 25%. The Benjamini-Hochberg method was used to correct the significant difference level (FDR).
The screening criteria for significantly different genes were: |Fold Change|>1.5 and FDR<0.05.

label: biotin

source_name: The HaCaT cell lines were infected by overexpression lentiviruses vectors of GJB2 gene mutant p.Asp66His(D66H).

label_protocol: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 50-500ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).

growth_protocol: The cell lines were maintained using Gibco Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% FBS and 1% penicillin/streptomycin at 37°C in a humidified atmosphere containing 5% CO2.

extract_protocol: Trizol extraction of total RNA was performed according to the manufacturer's instructions.

treatment_protocol: The HaCaT cell lines were infected by overexpression lentiviruses vectors of GJB2 gene mutant p.Asp66His(D66H), and empty GV308 vector.

value: HaCaT

tag: genotype/variation


title: The HaCaT cell lines, overexpression of vectors of GJB2 gene mutant  p.Asp66His(D66H) 3

institute: Department of Dermatology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan


title: Wildtype mESCs TSA 2h EZH2

value: embryonic stem cells

value: wildtype


title: Sample 8_ago59_sperm_R2

value: 35 days after germination

value: sperm cells


title: GM12878-CTCF-ChIA-Drop-Rep1

description:
Sample 1
nuclear chromatin
CTCF-ChIA-Drop
GM12878-CTCF-pooled_comp.txt
GM12878-CTCF-pooled.bedgraph
GM12878-CTCF-pooled.hic


title: Wildtype mESCs TSA 2h H3K27me3


title: Sample 6_ago9_sperm_R2


title: Wildtype mESCs TSA 2h EED-K19ac


title: Sample 5_ago9_sperm_R1


title: Wildtype mESCs P300

value: culture with LIF

value: P300 (Santa Cruz, sc-585)


title: ddi-1(icb156) L4 biol rep2

city: London

first: Michalis

last: Barkoulas

address: SAF Building, South Kensington

country: United Kingdom

institute: Imperial College London

data_processing:
Reads were aligned to C. elegans genome WS283
Reads were counted using Kallisto
Transcript abundance was quantified using the Sleuth and Wald test
Assembly: WormBase Release WS283
Supplementary files format and content: H5 files having raw TPM counts for all genes

organism: Caenorhabditis elegans

source_name: Whole animal

growth_protocol: Wild-type and ddi-1(icb156) worm strains were prepared by bleaching gravid adults to isolate embyros which were grown until L4 stage

extract_protocol:
TRIzol (Life Technologies)
By BGI

treatment_protocol: Nematode Growth Media agar plates supplemented with E. coli OP50 at 20°C

value: Whole animal

tag: age


title: ddi-1(icb156) L4 biol rep3


title: Wildtype mESCs EZH2


title: The HaCaT cell lines, overexpression of vectors of GJB2 gene mutant  p.Asp66His(D66H) 1


title: The HaCaT cell lines, overexpression lentiviruses empty vectors 3

source_name: The HaCaT cell lines were infected by overexpression lentiviruses empty vectors

value: no


title: ddi-1(icb156) L4 biol rep1


title: Sample 4_ago5_sperm_R2


title: The HaCaT cell lines, overexpression lentiviruses empty vectors 2


title: GM12878-CTCF-ChIA-Drop-Rep2

description:
Sample 2
nuclear chromatin
CTCF-ChIA-Drop
GM12878-CTCF-pooled_comp.txt
GM12878-CTCF-pooled.bedgraph
GM12878-CTCF-pooled.hic


title: wild-type L4 biol rep3

value: WT


title: The HaCaT cell lines, overexpression lentiviruses empty vectors 1


title: EED knockout mESCs EED-K19ac

value: EED knockout mutant


title: Wildtype mESCs H3K27me3


title: Sample 7_ago59_sperm_R1


title: wild-type L4 biol rep2


title: Sample 2_wt21_sperm_R2


title: The HaCaT cell lines, overexpression of vectors of GJB2 gene mutant p.Gly130Val(G130V) 3

source_name: The HaCaT cell lines were infected by overexpression lentiviruses vectors of GJB2 gene mutant p.Gly130Val(G130V).

treatment_protocol: The HaCaT cell lines were infected by overexpression lentiviruses vectors of GJB2 gene mutant G130V and empty GV308 vector.


title: Sample 3_ago5_sperm_R1

source_name: pollen


title: Wildtype mESCs EED-K19ac


title: The HaCaT cell lines, overexpression of vectors of GJB2 gene mutant p.Gly130Val(G130V) 2


title: wild-type L4 biol rep1


title: Sample 1_wt21_sperm_R1


title: The HaCaT cell lines, overexpression of vectors of GJB2 gene mutant p.Gly130Val(G130V) 1


title: rabbit-3

status: Public on Sep 29 2023

city: Beijing

first: wang

last: jiawen

address: 7 Science Park Road ZGC Life Science Park

institute: National institute of biological sciences

data_processing:
Basecalls performed using CASAVA version 1.9
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to GRCm38 (for mouse), Rnor6 (for rat), OryCun2 (for rabbit),OchPri3 (for pika) whole genome using STAR v2.6.1a with default parameters
Fragments Per Kilobase of exon per Megabase of library size (RPKM) were calculated using featureCounts function from Rsubread (v2.0.1) and rpkm function of edgeR (v3.28.1). In short, exons from all isoforms of a gene were merged to create one meta-transcript. The number of fragments falling in the exons of this meta-transcript were counted and normalized by the size of the meta-transcript and by the size of the library.
Genome_build: GRCm38, Rnor6, OryCun2, OchPri3
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample ...

organism: Oryctolagus cuniculus

source_name: rabbit fibroblast

growth_protocol: Cells were cultured under 5% CO2, 37 ℃ with DMEM (10% FBS, 1% PS)

extract_protocol:
After growing to 90% confluency, cells were lysed with TRIZOL directly and total RNA was extracted with chloroform and  isopropanol.
RNA libraries were prepared for sequencing using standard Illumina protocols

value: White New Zealand rabbit

value: epidermis


title: rabbit-2


title: rabbit-1


title: V2a_sample2_354_P13_R1

city: Stockholm

first: Abdel

last: El Manira

address: Solnavagen 9

department: Neuroscience

country: Sweden

institute: Karolinaska Institutet

data_processing:
The reads from each sequenced cell were mapped to the zebrafish reference genome “Danio_rerio, Ensembl, GRCz11” using STAR (version 2.5.3a).
The resulting bam files were filtered to keep only uniquely mapped reads.
Most of the following analysis was performed in R (version 4.0.5, R core team, 2022) using the Seurat package (version 4.0.2).
Assembly: GRCz11
Supplementary files format and content: .csv files with gene count matrixes; .txt and .csv metadata files and .rds files containing R objects from Seurat analysis

molecule: polyA RNA

organism: Danio rerio

source_name: Spinal cord

extract_protocol:
Adult animals (7 weeks old) of either sex were deeply anesthetized in a slush of frozen extracellular solution containing (in mM): 134 NaCl, 2.9 KCl, 2.1 CaCl2, 1.2 MgCl2, 10 HEPES and 10 glucose, with pH of 7.8 adjusted with NaOH and osmolarity of 290 mOsm. The spinal cord was quickly dissected in the slush of frozen extracellular solution and collected. Two samples were prepared from the Tg(islet1a:GFP) line, and two samples were prepared from the Tg(chx10:GFP) line. For each sample, 6 to 10 intact isolated spinal cords were incubated in 1 ml of DMEM-F12 medium (Thermo Fisher, #11039021, osmolarity adjusted to 280-280 mOsm) containing papain 10 U/ml (Worthington-biochem, #LK003178) on a heated shaker at 37°C for 15 min. DMEM/F-12 (1 ml, 280-290 mOsm) was added to stop the enzymatic reaction. The sample was centrifuged at 300 g at 4°C for 5 min, and then re-suspended in 0.5 ml of DMEM/F-12 (280-290 mOsm) after removal of the supernatant. Following mechanical trituration using fire-polished Pasteur pipettes, the cell suspension was filtered through a cell 16 strainer (40 μm). The sample was kept at room temperature for 20 min after the addition of 0,1 ml of the nuclear DNA stain DRAQ5 (Thermo Fisher, #65-0880-92). Using fluorescence activated cell sorting (FACs), cells positive for GFP and DRAQ5 in each sample were sorted into a 384-wells plate containing a mild hypotonic lysis buffer (0.2% Triton X-100, 2 U/ml RNase inhibitor) and immediately snap-frozen on ice, then stored at -80°C.
Smart-Seq2

value: Spinal cord

value: V2a interneurons


title: V2a_sample2_354_P12_R1


title: V2a_sample2_354_P22_R1


title: V2a_sample2_354_P18_R1


title: V2a_sample2_354_P17_R1


title: V2a_sample2_354_P11_R1


title: V2a_sample2_354_P23_R1


title: V2a_sample2_354_P19_R1


title: V2a_sample2_354_P15_R1


title: V2a_sample2_354_P20_R1


title: V2a_sample2_354_P9_R1


title: V2a_sample2_354_P14_R1


title: V2a_sample2_354_P21_R1


title: V2a_sample2_354_P24_R1


title: V2a_sample2_354_P5_R1


title: V2a_sample2_354_P16_R1


title: V2a_sample2_354_P10_R1


title: V2a_sample2_354_P4_R1


title: V2a_sample2_354_P3_R1


title: V2a_sample2_354_P2_R1


title: V2a_sample2_354_P6_R1


title: V2a_sample2_354_O24_R1


title: V2a_sample2_354_P1_R1


title: V2a_sample2_354_O22_R1


title: V2a_sample2_354_O23_R1


title: V2a_sample2_354_O21_R1


title: V2a_sample2_354_P7_R1


title: V2a_sample2_354_O20_R1


title: V2a_sample2_354_O18_R1


title: V2a_sample2_354_O19_R1


title: V2a_sample2_354_O16_R1


title: V2a_sample2_354_O14_R1


title: V2a_sample2_354_O13_R1


title: V2a_sample2_354_O15_R1


title: V2a_sample2_354_O12_R1


title: V2a_sample2_354_O10_R1


title: V2a_sample2_354_O8_R1


title: V2a_sample2_354_O9_R1


title: V2a_sample2_354_O11_R1


title: V2a_sample2_354_N24_R1


title: V2a_sample2_354_O4_R1


title: V2a_sample2_354_O6_R1


title: V2a_sample2_354_O2_R1


title: V2a_sample2_354_N20_R1


title: V2a_sample2_354_N21_R1


title: V2a_sample2_354_N23_R1


title: V2a_sample2_354_O1_R1


title: V2a_sample2_354_N18_R1


title: V2a_sample2_354_N17_R1


title: V2a_sample2_354_O3_R1


title: V2a_sample2_354_N19_R1


title: V2a_sample2_354_N22_R1


title: V2a_sample2_354_O17_R1


title: V2a_sample2_354_N16_R1


title: V2a_sample2_354_O7_R1


title: V2a_sample2_354_N15_R1


title: V2a_sample2_354_O5_R1


title: V2a_sample2_354_N12_R1


title: V2a_sample2_354_N10_R1


title: V2a_sample2_354_N11_R1


title: V2a_sample2_354_N8_R1


title: V2a_sample2_354_N14_R1


title: V2a_sample2_354_N9_R1


title: V2a_sample2_354_N7_R1


title: V2a_sample2_354_N6_R1


title: V2a_sample2_354_N5_R1


title: V2a_sample2_354_N3_R1


title: V2a_sample2_354_N1_R1


title: V2a_sample2_354_N2_R1


title: V2a_sample2_354_N4_R1


title: V2a_sample2_354_M22_R1


title: V2a_sample2_354_M24_R1


title: V2a_sample2_354_M23_R1


title: V2a_sample2_354_M21_R1


title: V2a_sample2_354_M20_R1


title: V2a_sample2_354_M19_R1


title: V2a_sample2_354_M18_R1


title: V2a_sample2_354_M17_R1


title: V2a_sample2_354_M16_R1


title: V2a_sample2_354_N13_R1


title: V2a_sample2_354_M13_R1


title: V2a_sample2_354_M14_R1


title: V2a_sample2_354_M15_R1


title: V2a_sample2_354_M12_R1


title: V2a_sample2_354_M11_R1


title: V2a_sample2_354_M10_R1


title: V2a_sample2_354_M8_R1


title: V2a_sample2_354_M9_R1


title: V2a_sample2_354_M5_R1


title: V2a_sample2_354_M7_R1


title: V2a_sample2_354_M2_R1


title: V2a_sample2_354_M3_R1


title: V2a_sample2_354_M6_R1


title: V2a_sample2_354_M4_R1


title: V2a_sample2_354_L20_R1


title: V2a_sample2_354_M1_R1


title: V2a_sample2_354_L23_R1


title: V2a_sample2_354_L22_R1


title: V2a_sample2_354_L18_R1


title: V2a_sample2_354_L21_R1


title: V2a_sample2_354_L19_R1


title: V2a_sample2_354_L24_R1


title: V2a_sample2_354_L15_R1


title: V2a_sample2_354_L12_R1


title: V2a_sample2_354_L16_R1


title: V2a_sample2_354_L14_R1


title: V2a_sample2_354_L11_R1


title: V2a_sample2_354_L13_R1


title: V2a_sample2_354_L8_R1


title: V2a_sample2_354_L10_R1


title: V2a_sample2_354_L7_R1


title: V2a_sample2_354_L9_R1


title: V2a_sample2_354_L17_R1


title: V2a_sample2_354_L3_R1


title: V2a_sample2_354_L4_R1


title: V2a_sample2_354_L5_R1


title: V2a_sample2_354_L6_R1


title: V2a_sample2_354_L2_R1


title: V2a_sample2_354_K23_R1


title: V2a_sample2_354_K24_R1


title: V2a_sample2_354_K22_R1


title: V2a_sample2_354_K21_R1


title: V2a_sample2_354_K19_R1


title: V2a_sample2_354_K18_R1


title: V2a_sample2_354_K15_R1


title: V2a_sample2_354_L1_R1


title: V2a_sample2_354_K17_R1


title: V2a_sample2_354_K20_R1


title: V2a_sample2_354_K14_R1


title: V2a_sample2_354_K13_R1


title: V2a_sample2_354_K16_R1


title: V2a_sample2_354_K10_R1


title: V2a_sample2_354_K11_R1


title: V2a_sample2_354_K7_R1


title: V2a_sample2_354_K12_R1


title: V2a_sample2_354_K8_R1


title: V2a_sample2_354_K9_R1


title: V2a_sample2_354_K4_R1


title: V2a_sample2_354_K6_R1


title: V2a_sample2_354_J23_R1


title: V2a_sample2_354_J20_R1


title: V2a_sample2_354_K3_R1


title: V2a_sample2_354_K5_R1


title: V2a_sample2_354_K2_R1


title: V2a_sample2_354_K1_R1


title: V2a_sample2_354_J24_R1


title: V2a_sample2_354_J18_R1


title: V2a_sample2_354_J16_R1


title: V2a_sample2_354_J14_R1


title: V2a_sample2_354_J13_R1


title: V2a_sample2_354_J22_R1


title: V2a_sample2_354_J21_R1


title: V2a_sample2_354_J6_R1


title: V2a_sample2_354_J7_R1


title: V2a_sample2_354_J5_R1


title: V2a_sample2_354_J3_R1


title: V2a_sample2_354_J1_R1


title: V2a_sample2_354_J17_R1


title: V2a_sample2_354_J19_R1


title: V2a_sample2_354_J15_R1


title: V2a_sample2_354_I24_R1


title: V2a_sample2_354_J12_R1


title: V2a_sample2_354_J11_R1


title: V2a_sample2_354_J10_R1


title: V2a_sample2_354_J9_R1


title: V2a_sample2_354_P8_R1


title: V2a_sample2_354_I20_R1


title: V2a_sample2_354_J8_R1


title: V2a_sample2_354_I18_R1


title: V2a_sample2_354_I17_R1


title: V2a_sample2_354_J4_R1


title: V2a_sample2_354_J2_R1


title: V2a_sample2_354_I23_R1


title: V2a_sample2_354_I8_R1


title: V2a_sample2_354_I22_R1


title: V2a_sample2_354_I21_R1


title: V2a_sample2_354_I3_R1


title: V2a_sample2_354_I14_R1


title: V2a_sample2_354_I9_R1


title: V2a_sample2_354_I16_R1


title: V2a_sample2_354_I7_R1


title: V2a_sample2_354_I2_R1


title: V2a_sample2_354_I1_R1


title: V2a_sample2_354_I6_R1


title: V2a_sample2_354_I19_R1


title: V2a_sample2_354_H23_R1


title: V2a_sample2_354_I5_R1


title: V2a_sample2_354_I4_R1


title: V2a_sample2_354_H18_R1


title: V2a_sample2_354_H24_R1


title: V2a_sample2_354_I12_R1


title: V2a_sample2_354_H21_R1


title: V2a_sample2_354_I10_R1


title: V2a_sample2_354_H17_R1


title: V2a_sample2_354_H19_R1


title: V2a_sample2_354_H22_R1


title: V2a_sample2_354_I11_R1


title: V2a_sample2_354_I13_R1


title: V2a_sample2_354_H20_R1


title: V2a_sample2_354_H14_R1


title: V2a_sample2_354_H15_R1


title: V2a_sample2_354_I15_R1


title: V2a_sample2_354_H12_R1


title: V2a_sample2_354_H11_R1


title: V2a_sample2_354_H9_R1


title: V2a_sample2_354_H10_R1


title: V2a_sample2_354_H13_R1


title: V2a_sample2_354_H6_R1


title: V2a_sample2_354_H3_R1


title: V2a_sample2_354_H1_R1


title: V2a_sample2_354_H2_R1


title: V2a_sample2_354_H16_R1


title: V2a_sample2_354_G18_R1


title: V2a_sample2_354_G22_R1


title: V2a_sample2_354_G20_R1


title: V2a_sample2_354_G19_R1


title: V2a_sample2_354_G15_R1


title: V2a_sample2_354_G16_R1


title: V2a_sample2_354_G14_R1


title: V2a_sample2_354_G13_R1


title: V2a_sample2_354_H7_R1


title: V2a_sample2_354_G9_R1


title: V2a_sample2_354_G12_R1


title: V2a_sample2_354_H8_R1


title: V2a_sample2_354_G11_R1


title: V2a_sample2_354_H4_R1


title: V2a_sample2_354_G4_R1


title: V2a_sample2_354_G23_R1


title: V2a_sample2_354_G3_R1


title: V2a_sample2_354_G2_R1


title: V2a_sample2_354_G6_R1


title: V2a_sample2_354_G7_R1


title: V2a_sample2_354_G21_R1


title: V2a_sample2_354_H5_R1


title: V2a_sample2_354_G17_R1


title: V2a_sample2_354_G5_R1


title: V2a_sample2_354_G1_R1


title: V2a_sample2_354_G24_R1


title: V2a_sample2_354_F23_R1


title: V2a_sample2_354_F24_R1


title: V2a_sample2_354_F19_R1


title: V2a_sample2_354_F20_R1


title: V2a_sample2_354_G10_R1


title: V2a_sample2_354_G8_R1


title: V2a_sample2_354_F22_R1


title: V2a_sample2_354_F14_R1


title: V2a_sample2_354_F12_R1


title: V2a_sample2_354_F15_R1


title: V2a_sample2_354_F16_R1


title: V2a_sample2_354_F11_R1


title: V2a_sample2_354_F21_R1


title: V2a_sample2_354_F10_R1


title: V2a_sample2_354_F8_R1


title: V2a_sample2_354_F9_R1


title: V2a_sample2_354_F7_R1


title: V2a_sample2_354_F13_R1


title: V2a_sample2_354_F6_R1


title: V2a_sample2_354_F3_R1


title: V2a_sample2_354_E24_R1


title: V2a_sample2_354_F18_R1


title: V2a_sample2_354_F4_R1


title: V2a_sample2_354_F17_R1


title: V2a_sample2_354_F5_R1


title: V2a_sample2_354_F1_R1


title: V2a_sample2_354_F2_R1


title: V2a_sample2_354_E21_R1


title: V2a_sample2_354_E17_R1


title: V2a_sample2_354_E20_R1


title: V2a_sample2_354_E18_R1


title: V2a_sample2_354_E19_R1


title: V2a_sample2_354_E16_R1


title: V2a_sample2_354_E15_R1


title: V2a_sample2_354_E13_R1


title: V2a_sample2_354_E14_R1


title: V2a_sample2_354_E23_R1


title: V2a_sample2_354_E12_R1


title: V2a_sample2_354_E9_R1


title: V2a_sample2_354_E11_R1


title: V2a_sample2_354_E10_R1


title: V2a_sample2_354_E22_R1


title: V2a_sample2_354_E7_R1


title: V2a_sample2_354_E5_R1


title: V2a_sample2_354_E2_R1


title: V2a_sample2_354_E4_R1


title: V2a_sample2_354_E8_R1


title: V2a_sample2_354_E3_R1


title: V2a_sample2_354_E1_R1


title: V2a_sample2_354_D23_R1


title: V2a_sample2_354_D19_R1


title: V2a_sample2_354_D21_R1


title: V2a_sample2_354_D18_R1


title: V2a_sample2_354_D20_R1


title: V2a_sample2_354_D24_R1


title: V2a_sample2_354_D16_R1


title: V2a_sample2_354_D17_R1


title: V2a_sample2_354_D14_R1


title: V2a_sample2_354_D15_R1


title: V2a_sample2_354_D12_R1


title: V2a_sample2_354_D9_R1


title: V2a_sample2_354_D10_R1


title: V2a_sample2_354_D13_R1


title: V2a_sample2_354_E6_R1


title: V2a_sample2_354_D8_R1


title: V2a_sample2_354_D22_R1


title: V2a_sample2_354_D5_R1


title: V2a_sample2_354_D3_R1


title: V2a_sample2_354_D6_R1


title: V2a_sample2_354_D4_R1


title: V2a_sample2_354_D2_R1


title: V2a_sample2_354_C24_R1


title: V2a_sample2_354_D7_R1


title: V2a_sample2_354_C21_R1


title: V2a_sample2_354_D1_R1


title: V2a_sample2_354_C19_R1


title: V2a_sample2_354_C18_R1


title: V2a_sample2_354_C23_R1


title: V2a_sample2_354_C14_R1


title: V2a_sample2_354_C20_R1


title: V2a_sample2_354_C22_R1


title: V2a_sample2_354_C15_R1


title: V2a_sample2_354_C13_R1


title: V2a_sample2_354_C10_R1


title: V2a_sample2_354_C17_R1


title: V2a_sample2_354_C16_R1


title: V2a_sample2_354_C11_R1


title: V2a_sample2_354_C9_R1


title: V2a_sample2_354_C12_R1


title: V2a_sample2_354_C7_R1


title: V2a_sample2_354_C8_R1


title: V2a_sample2_354_C6_R1


title: V2a_sample2_354_C5_R1


title: V2a_sample2_354_C4_R1


title: V2a_sample2_354_C3_R1


title: V2a_sample2_354_C2_R1


title: V2a_sample2_354_C1_R1


title: V2a_sample2_354_B24_R1


title: V2a_sample2_354_B23_R1


title: V2a_sample2_354_B22_R1


title: V2a_sample2_354_B21_R1


title: V2a_sample2_354_B19_R1


title: V2a_sample2_354_B17_R1


title: V2a_sample2_354_B16_R1


title: V2a_sample2_354_B18_R1


title: V2a_sample2_354_B14_R1


title: V2a_sample2_354_B13_R1


title: V2a_sample2_354_B12_R1


title: V2a_sample2_354_D11_R1


title: V2a_sample2_354_B15_R1


title: V2a_sample2_354_B20_R1


title: V2a_sample2_354_A21_R1


title: V2a_sample2_354_B4_R1


title: V2a_sample2_354_B10_R1


title: V2a_sample2_354_B2_R1


title: V2a_sample2_354_A23_R1


title: V2a_sample2_354_B11_R1


title: V2a_sample2_354_A20_R1


title: V2a_sample2_354_B3_R1


title: V2a_sample2_354_A22_R1


title: V2a_sample2_354_B6_R1


title: V2a_sample2_354_B5_R1


title: V2a_sample2_354_A24_R1


title: V2a_sample2_354_B7_R1


title: V2a_sample2_354_B1_R1


title: V2a_sample2_354_B9_R1


title: V2a_sample2_354_A12_R1


title: V2a_sample2_354_A13_R1


title: V2a_sample2_354_B8_R1


title: V2a_sample2_354_A9_R1


title: V2a_sample2_354_A11_R1


title: V2a_sample2_354_A19_R1


title: V2a_sample2_354_A18_R1


title: V2a_sample2_354_A14_R1


title: V2a_sample2_354_A8_R1


title: V2a_sample2_354_A15_R1


title: V2a_sample2_354_A7_R1


title: V2a_sample2_354_A6_R1


title: V2a_sample2_354_A17_R1


title: V2a_sample2_354_A16_R1


title: V2a_sample2_354_A10_R1


title: V2a_sample2_354_A5_R1


title: V2a_sample1_353_P16_R1


title: V2a_sample1_353_P17_R1


title: V2a_sample1_353_P18_R1


title: V2a_sample2_354_A4_R1


title: V2a_sample1_353_P23_R1


title: V2a_sample2_354_A3_R1


title: V2a_sample1_353_P22_R1


title: V2a_sample1_353_P21_R1


title: V2a_sample1_353_P13_R1


title: V2a_sample1_353_P12_R1


title: V2a_sample1_353_P15_R1


title: V2a_sample1_353_P11_R1


title: V2a_sample2_354_A2_R1


title: V2a_sample1_353_P8_R1


title: V2a_sample1_353_P24_R1


title: V2a_sample2_354_A1_R1


title: V2a_sample1_353_P5_R1


title: V2a_sample1_353_P6_R1


title: V2a_sample1_353_P14_R1


title: V2a_sample1_353_P19_R1


title: V2a_sample1_353_P4_R1


title: V2a_sample1_353_P7_R1


title: V2a_sample1_353_P9_R1


title: V2a_sample1_353_P3_R1


title: V2a_sample1_353_P10_R1


title: V2a_sample1_353_P2_R1


title: V2a_sample1_353_P1_R1


title: V2a_sample1_353_P20_R1


title: V2a_sample1_353_O24_R1


title: V2a_sample1_353_O23_R1


title: V2a_sample1_353_O22_R1


title: V2a_sample1_353_O20_R1


title: V2a_sample1_353_O18_R1


title: V2a_sample1_353_O21_R1


title: V2a_sample1_353_O19_R1


title: V2a_sample1_353_O9_R1


title: V2a_sample1_353_O10_R1


title: V2a_sample1_353_O8_R1


title: V2a_sample1_353_O3_R1


title: V2a_sample1_353_O17_R1


title: V2a_sample1_353_O13_R1


title: V2a_sample1_353_N24_R1


title: V2a_sample1_353_O1_R1


title: V2a_sample1_353_O16_R1


title: V2a_sample1_353_O6_R1


title: V2a_sample1_353_N22_R1


title: V2a_sample1_353_O5_R1


title: V2a_sample1_353_O14_R1


title: V2a_sample1_353_O12_R1


title: V2a_sample1_353_O15_R1


title: V2a_sample1_353_O7_R1


title: V2a_sample1_353_O11_R1


title: V2a_sample1_353_O2_R1


title: V2a_sample1_353_N23_R1


title: V2a_sample1_353_O4_R1


title: V2a_sample1_353_N18_R1


title: V2a_sample1_353_N21_R1


title: V2a_sample1_353_N19_R1


title: V2a_sample1_353_N20_R1


title: V2a_sample1_353_N17_R1


title: V2a_sample1_353_N15_R1


title: V2a_sample1_353_N14_R1


title: V2a_sample1_353_N13_R1


title: V2a_sample1_353_N10_R1


title: V2a_sample1_353_N8_R1


title: V2a_sample1_353_N9_R1


title: V2a_sample1_353_N2_R1


title: V2a_sample1_353_M23_R1


title: V2a_sample1_353_N1_R1


title: V2a_sample1_353_M21_R1


title: V2a_sample1_353_N6_R1


title: V2a_sample1_353_M24_R1


title: V2a_sample1_353_M22_R1


title: V2a_sample1_353_N12_R1


title: V2a_sample1_353_M20_R1


title: V2a_sample1_353_M18_R1


title: V2a_sample1_353_M19_R1


title: V2a_sample1_353_N11_R1


title: V2a_sample1_353_M17_R1


title: V2a_sample1_353_M13_R1


title: V2a_sample1_353_M15_R1


title: V2a_sample1_353_M16_R1


title: V2a_sample1_353_M14_R1


title: V2a_sample1_353_N4_R1


title: V2a_sample1_353_N5_R1


title: V2a_sample1_353_M12_R1


title: V2a_sample1_353_N16_R1


title: V2a_sample1_353_M11_R1


title: V2a_sample1_353_M10_R1


title: V2a_sample1_353_M8_R1


title: V2a_sample1_353_M9_R1


title: V2a_sample1_353_M6_R1


title: V2a_sample1_353_M5_R1


title: V2a_sample1_353_M7_R1


title: V2a_sample1_353_N3_R1


title: V2a_sample1_353_M2_R1


title: V2a_sample1_353_M1_R1


title: V2a_sample1_353_N7_R1


title: V2a_sample1_353_M4_R1


title: V2a_sample1_353_L21_R1


title: V2a_sample1_353_L22_R1


title: V2a_sample1_353_L23_R1


title: V2a_sample1_353_L24_R1


title: V2a_sample1_353_L17_R1


title: V2a_sample1_353_L16_R1


title: V2a_sample1_353_L19_R1


title: V2a_sample1_353_L18_R1


title: V2a_sample1_353_L12_R1


title: V2a_sample1_353_L15_R1


title: V2a_sample1_353_L11_R1


title: V2a_sample1_353_L14_R1


title: V2a_sample1_353_M3_R1


title: V2a_sample1_353_L20_R1


title: V2a_sample1_353_L9_R1


title: V2a_sample1_353_L13_R1


title: V2a_sample1_353_L10_R1


title: V2a_sample1_353_L6_R1


title: V2a_sample1_353_L4_R1


title: V2a_sample1_353_L5_R1


title: V2a_sample1_353_L3_R1


title: V2a_sample1_353_L2_R1


title: V2a_sample1_353_K24_R1


title: V2a_sample1_353_K22_R1


title: V2a_sample1_353_K23_R1


title: V2a_sample1_353_K21_R1


title: V2a_sample1_353_K19_R1


title: V2a_sample1_353_K16_R1


title: V2a_sample1_353_K18_R1


title: V2a_sample1_353_K14_R1


title: V2a_sample1_353_K8_R1


title: V2a_sample1_353_K17_R1


title: V2a_sample1_353_K7_R1


title: V2a_sample1_353_K11_R1


title: V2a_sample1_353_K9_R1


title: V2a_sample1_353_L7_R1


title: V2a_sample1_353_K12_R1


title: V2a_sample1_353_K6_R1


title: V2a_sample1_353_K10_R1


title: V2a_sample1_353_K13_R1


title: V2a_sample1_353_K5_R1


title: V2a_sample1_353_K4_R1


title: V2a_sample1_353_L1_R1


title: V2a_sample1_353_J24_R1


title: V2a_sample1_353_K1_R1


title: V2a_sample1_353_K3_R1


title: V2a_sample1_353_K20_R1


title: V2a_sample1_353_K2_R1


title: V2a_sample1_353_J23_R1


title: V2a_sample1_353_J22_R1


title: V2a_sample1_353_J21_R1


title: V2a_sample1_353_J20_R1


title: V2a_sample1_353_J17_R1


title: V2a_sample1_353_J16_R1


title: V2a_sample1_353_J14_R1


title: V2a_sample1_353_K15_R1


title: V2a_sample1_353_J12_R1


title: V2a_sample1_353_J13_R1


title: V2a_sample1_353_J10_R1


title: V2a_sample1_353_J9_R1


title: V2a_sample1_353_J6_R1


title: V2a_sample1_353_J7_R1


title: V2a_sample1_353_J8_R1


title: V2a_sample1_353_J5_R1


title: V2a_sample1_353_J3_R1


title: V2a_sample1_353_I23_R1


title: V2a_sample1_353_J1_R1


title: V2a_sample1_353_I21_R1


title: V2a_sample1_353_J19_R1


title: V2a_sample1_353_J18_R1


title: V2a_sample1_353_J15_R1


title: V2a_sample1_353_I20_R1


title: V2a_sample1_353_I18_R1


title: V2a_sample1_353_J11_R1


title: V2a_sample1_353_I16_R1


title: V2a_sample1_353_J4_R1


title: V2a_sample1_353_I14_R1


title: V2a_sample1_353_I12_R1


title: V2a_sample1_353_I13_R1


title: V2a_sample1_353_I11_R1


title: V2a_sample1_353_J2_R1


title: V2a_sample1_353_I9_R1


title: V2a_sample1_353_I24_R1


title: V2a_sample1_353_I4_R1


title: V2a_sample1_353_I10_R1


title: V2a_sample1_353_I22_R1


title: V2a_sample1_353_I7_R1


title: V2a_sample1_353_I5_R1


title: V2a_sample1_353_I3_R1


title: V2a_sample1_353_I1_R1


title: V2a_sample1_353_H24_R1


title: V2a_sample1_353_I19_R1


title: V2a_sample1_353_I17_R1


title: V2a_sample1_353_H23_R1


title: V2a_sample1_353_H21_R1


title: V2a_sample1_353_H22_R1


title: V2a_sample1_353_I15_R1


title: V2a_sample1_353_H20_R1


title: V2a_sample1_353_H18_R1


title: V2a_sample1_353_H19_R1


title: V2a_sample1_353_H17_R1


title: V2a_sample1_353_H16_R1


title: V2a_sample1_353_I8_R1


title: V2a_sample1_353_I6_R1


title: V2a_sample1_353_H14_R1


title: V2a_sample1_353_H13_R1


title: V2a_sample1_353_H11_R1


title: V2a_sample1_353_H10_R1


title: V2a_sample1_353_I2_R1


title: V2a_sample1_353_H8_R1


title: V2a_sample1_353_H9_R1


title: V2a_sample1_353_H6_R1


title: V2a_sample1_353_H7_R1


title: V2a_sample1_353_H4_R1


title: V2a_sample1_353_H2_R1


title: V2a_sample1_353_H3_R1


title: V2a_sample1_353_H1_R1


title: V2a_sample1_353_G23_R1


title: V2a_sample1_353_G20_R1


title: V2a_sample1_353_G19_R1


title: V2a_sample1_353_G24_R1


title: V2a_sample1_353_G17_R1


title: V2a_sample1_353_G22_R1


title: V2a_sample1_353_G21_R1


title: V2a_sample1_353_H15_R1


title: V2a_sample1_353_G16_R1


title: V2a_sample1_353_H12_R1


title: V2a_sample1_353_G12_R1


title: V2a_sample1_353_G14_R1


title: V2a_sample1_353_G13_R1


title: V2a_sample1_353_G11_R1


title: V2a_sample1_353_H5_R1


title: V2a_sample1_353_G9_R1


title: V2a_sample1_353_G10_R1


title: V2a_sample1_353_G6_R1


title: V2a_sample1_353_G8_R1


title: V2a_sample1_353_G4_R1


title: V2a_sample1_353_G5_R1


title: V2a_sample1_353_G7_R1


title: V2a_sample1_353_G2_R1


title: V2a_sample1_353_G1_R1


title: V2a_sample1_353_G3_R1


title: V2a_sample1_353_F24_R1


title: V2a_sample1_353_G18_R1


title: V2a_sample1_353_F23_R1


title: V2a_sample1_353_F22_R1


title: V2a_sample1_353_F21_R1


title: V2a_sample1_353_F20_R1


title: V2a_sample1_353_F19_R1


title: V2a_sample1_353_F15_R1


title: V2a_sample1_353_F14_R1


title: V2a_sample1_353_G15_R1


title: V2a_sample1_353_F18_R1


title: V2a_sample1_353_F13_R1


title: V2a_sample1_353_F17_R1


title: V2a_sample1_353_F12_R1


title: V2a_sample1_353_F11_R1


title: V2a_sample1_353_F16_R1


title: V2a_sample1_353_F10_R1


title: V2a_sample1_353_F9_R1


title: V2a_sample1_353_F8_R1


title: V2a_sample1_353_F7_R1


title: V2a_sample1_353_L8_R1


title: V2a_sample1_353_F5_R1


title: V2a_sample1_353_F4_R1


title: V2a_sample1_353_F3_R1


title: V2a_sample1_353_F2_R1


title: V2a_sample1_353_E24_R1


title: V2a_sample1_353_F1_R1


title: V2a_sample1_353_E23_R1


title: V2a_sample1_353_E22_R1


title: V2a_sample1_353_E21_R1


title: V2a_sample1_353_E20_R1


title: V2a_sample1_353_E18_R1


title: V2a_sample1_353_E19_R1


title: V2a_sample1_353_E17_R1


title: V2a_sample1_353_E16_R1


title: V2a_sample1_353_F6_R1


title: V2a_sample1_353_D19_R1


title: V2a_sample1_353_D21_R1


title: V2a_sample1_353_D24_R1


title: V2a_sample1_353_D18_R1


title: V2a_sample1_353_E6_R1


title: V2a_sample1_353_E4_R1


title: V2a_sample1_353_E14_R1


title: V2a_sample1_353_E8_R1


title: V2a_sample1_353_E1_R1


title: V2a_sample1_353_E3_R1


title: V2a_sample1_353_E11_R1


title: V2a_sample1_353_E2_R1


title: V2a_sample1_353_D20_R1


title: V2a_sample1_353_E5_R1


title: V2a_sample1_353_E10_R1


title: V2a_sample1_353_E12_R1


title: V2a_sample1_353_E9_R1


title: V2a_sample1_353_E15_R1


title: V2a_sample1_353_D22_R1


title: V2a_sample1_353_D15_R1


title: V2a_sample1_353_E13_R1


title: V2a_sample1_353_E7_R1


title: V2a_sample1_353_D14_R1


title: V2a_sample1_353_D13_R1


title: V2a_sample1_353_D12_R1


title: V2a_sample1_353_D11_R1


title: V2a_sample1_353_D23_R1


title: V2a_sample1_353_D16_R1


title: V2a_sample1_353_D17_R1


title: V2a_sample1_353_D10_R1


title: V2a_sample1_353_D8_R1


title: V2a_sample1_353_D9_R1


title: V2a_sample1_353_D4_R1


title: V2a_sample1_353_D5_R1


title: V2a_sample1_353_C15_R1


title: V2a_sample1_353_C16_R1


title: V2a_sample1_353_C21_R1


title: V2a_sample1_353_C20_R1


title: V2a_sample1_353_D6_R1


title: V2a_sample1_353_C14_R1


title: V2a_sample1_353_C13_R1


title: V2a_sample1_353_C22_R1


title: V2a_sample1_353_C24_R1


title: V2a_sample1_353_D1_R1


title: V2a_sample1_353_D3_R1


title: V2a_sample1_353_C18_R1


title: V2a_sample1_353_C19_R1


title: V2a_sample1_353_C23_R1


title: V2a_sample1_353_D2_R1


title: V2a_sample1_353_C12_R1


title: V2a_sample1_353_C8_R1


title: V2a_sample1_353_C9_R1


title: V2a_sample1_353_C10_R1


title: V2a_sample1_353_C11_R1


title: V2a_sample1_353_C5_R1


title: V2a_sample1_353_C6_R1


title: V2a_sample1_353_D7_R1


title: V2a_sample1_353_C7_R1


title: V2a_sample1_353_C4_R1


title: V2a_sample1_353_C3_R1


title: V2a_sample1_353_C1_R1


title: V2a_sample1_353_B24_R1


title: V2a_sample1_353_B22_R1


title: V2a_sample1_353_B23_R1


title: V2a_sample1_353_B14_R1


title: V2a_sample1_353_B13_R1


title: V2a_sample1_353_B21_R1


title: V2a_sample1_353_B19_R1


title: V2a_sample1_353_B9_R1


title: V2a_sample1_353_B20_R1


title: V2a_sample1_353_B11_R1


title: V2a_sample1_353_B18_R1


title: V2a_sample1_353_B16_R1


title: V2a_sample1_353_B17_R1


title: V2a_sample1_353_B12_R1


title: V2a_sample1_353_B15_R1


title: V2a_sample1_353_B5_R1


title: V2a_sample1_353_B7_R1


title: V2a_sample1_353_B10_R1


title: V2a_sample1_353_B8_R1


title: V2a_sample1_353_B4_R1


title: V2a_sample1_353_B6_R1


title: V2a_sample1_353_A24_R1


title: V2a_sample1_353_A23_R1


title: V2a_sample1_353_C2_R1


title: V2a_sample1_353_B3_R1


title: V2a_sample1_353_A20_R1


title: V2a_sample1_353_B2_R1


title: V2a_sample1_353_B1_R1


title: V2a_sample1_353_A19_R1


title: V2a_sample1_353_A21_R1


title: V2a_sample1_353_A22_R1


title: V2a_sample1_353_A18_R1


title: V2a_sample1_353_A17_R1


title: V2a_sample1_353_A16_R1


title: V2a_sample1_353_A14_R1


title: V2a_sample1_353_A9_R1


title: V2a_sample1_353_A8_R1


title: V2a_sample1_353_A13_R1


title: V2a_sample1_353_A15_R1


title: V2a_sample1_353_A5_R1


title: V2a_sample1_353_A12_R1


title: V2a_sample1_353_A1_R1


title: V2a_sample1_353_A7_R1


title: V2a_sample1_353_A11_R1


title: V2a_sample1_353_A10_R1


title: V2a_sample1_353_A2_R1


title: MN_sample2_174_P22_R1

value: motoneurons


title: V2a_sample1_353_A3_R1


title: V2a_sample1_353_A4_R1


title: MN_sample2_174_P24_R1


title: V2a_sample1_353_A6_R1


title: MN_sample2_174_P23_R1


title: MN_sample2_174_P20_R1


title: MN_sample2_174_P19_R1


title: MN_sample2_174_P18_R1


title: MN_sample2_174_P21_R1


title: MN_sample2_174_P17_R1


title: MN_sample2_174_P16_R1


title: MN_sample2_174_P13_R1


title: MN_sample2_174_P15_R1


title: MN_sample2_174_P14_R1


title: MN_sample2_174_P12_R1


title: MN_sample2_174_P11_R1


title: MN_sample2_174_P10_R1


title: MN_sample2_174_P8_R1


title: MN_sample2_174_P9_R1


title: MN_sample2_174_P6_R1


title: MN_sample2_174_P4_R1


title: MN_sample2_174_P5_R1


title: MN_sample2_174_P7_R1


title: MN_sample2_174_P1_R1


title: MN_sample2_174_O24_R1


title: MN_sample2_174_P2_R1


title: MN_sample2_174_P3_R1


title: MN_sample2_174_O20_R1


title: MN_sample2_174_O18_R1


title: MN_sample2_174_O22_R1


title: MN_sample2_174_O19_R1


title: MN_sample2_174_O16_R1


title: MN_sample2_174_O17_R1


title: MN_sample2_174_O23_R1


title: MN_sample2_174_O11_R1


title: MN_sample2_174_O15_R1


title: MN_sample2_174_O12_R1


title: MN_sample2_174_O9_R1


title: MN_sample2_174_O21_R1


title: MN_sample2_174_O8_R1


title: MN_sample2_174_O10_R1


title: MN_sample2_174_O14_R1


title: MN_sample2_174_O6_R1


title: MN_sample2_174_O4_R1


title: MN_sample2_174_O7_R1


title: MN_sample2_174_O13_R1


title: MN_sample2_174_O3_R1


title: MN_sample2_174_O1_R1


title: MN_sample2_174_N23_R1


title: MN_sample2_174_N20_R1


title: MN_sample2_174_N21_R1


title: MN_sample2_174_N22_R1


title: MN_sample2_174_N15_R1


title: MN_sample2_174_N13_R1


title: MN_sample2_174_N16_R1


title: MN_sample2_174_N18_R1


title: MN_sample2_174_N17_R1


title: MN_sample2_174_N19_R1


title: MN_sample2_174_N14_R1


title: MN_sample2_174_O5_R1


title: MN_sample2_174_N9_R1


title: MN_sample2_174_N11_R1


title: MN_sample2_174_N12_R1


title: MN_sample2_174_N24_R1


title: MN_sample2_174_N7_R1


title: MN_sample2_174_N10_R1


title: MN_sample2_174_N4_R1


title: MN_sample2_174_O2_R1


title: MN_sample2_174_N6_R1


title: MN_sample2_174_N5_R1


title: MN_sample2_174_N3_R1


title: MN_sample2_174_M24_R1


title: MN_sample2_174_N2_R1


title: MN_sample2_174_N1_R1


title: MN_sample2_174_M20_R1


title: MN_sample2_174_M23_R1


title: MN_sample2_174_M22_R1


title: MN_sample2_174_M19_R1


title: MN_sample2_174_M18_R1


title: MN_sample2_174_M16_R1


title: MN_sample2_174_M15_R1


title: MN_sample2_174_M13_R1


title: MN_sample2_174_M11_R1


title: MN_sample2_174_M14_R1


title: MN_sample2_174_N8_R1


title: MN_sample2_174_M12_R1


title: MN_sample2_174_M10_R1


title: MN_sample2_174_M8_R1


title: MN_sample2_174_M9_R1


title: MN_sample2_174_M5_R1


title: MN_sample2_174_M6_R1


title: MN_sample2_174_M3_R1


title: MN_sample2_174_M2_R1


title: MN_sample2_174_M7_R1


title: MN_sample2_174_L23_R1


title: MN_sample2_174_L21_R1


title: MN_sample2_174_L22_R1


title: MN_sample2_174_L20_R1


title: MN_sample2_174_L19_R1


title: MN_sample2_174_L17_R1


title: MN_sample2_174_M17_R1


title: MN_sample2_174_L16_R1


title: MN_sample2_174_L15_R1


title: MN_sample2_174_L14_R1


title: MN_sample2_174_L12_R1


title: MN_sample2_174_L13_R1


title: MN_sample2_174_L9_R1


title: MN_sample2_174_L11_R1


title: V2a_sample1_353_C17_R1


title: MN_sample2_174_M4_R1


title: MN_sample2_174_L7_R1


title: MN_sample2_174_L24_R1


title: MN_sample2_174_M1_R1


title: MN_sample2_174_L2_R1


title: MN_sample2_174_L5_R1


title: MN_sample2_174_L1_R1


title: MN_sample2_174_L3_R1


title: MN_sample2_174_K23_R1


title: MN_sample2_174_L18_R1


title: MN_sample2_174_K22_R1


title: MN_sample2_174_K21_R1


title: MN_sample2_174_K19_R1


title: MN_sample2_174_K16_R1


title: MN_sample2_174_K17_R1


title: MN_sample2_174_K20_R1


title: MN_sample2_174_L10_R1


title: MN_sample2_174_K14_R1


title: MN_sample2_174_K15_R1


title: MN_sample2_174_K13_R1


title: MN_sample2_174_L8_R1


title: MN_sample2_174_L6_R1


title: MN_sample2_174_K10_R1


title: MN_sample2_174_K11_R1


title: MN_sample2_174_K12_R1


title: MN_sample2_174_K24_R1


title: MN_sample2_174_L4_R1


title: MN_sample2_174_K6_R1


title: MN_sample2_174_K4_R1


title: MN_sample2_174_K7_R1


title: MN_sample2_174_K5_R1


title: MN_sample2_174_K3_R1


title: MN_sample2_174_K9_R1


title: MN_sample2_174_K18_R1


title: MN_sample2_174_J22_R1


title: MN_sample2_174_J20_R1


title: MN_sample2_174_M21_R1


title: MN_sample2_174_K1_R1


title: MN_sample2_174_J23_R1


title: MN_sample2_174_J18_R1


title: MN_sample2_174_J16_R1


title: MN_sample2_174_K2_R1


title: MN_sample2_174_J12_R1


title: MN_sample2_174_J15_R1


title: MN_sample2_174_J10_R1


title: MN_sample2_174_J8_R1


title: MN_sample2_174_J11_R1


title: MN_sample2_174_J9_R1


title: MN_sample2_174_J6_R1


title: MN_sample2_174_J21_R1


title: MN_sample2_174_J7_R1


title: MN_sample2_174_J5_R1


title: MN_sample2_174_J1_R1


title: MN_sample2_174_J2_R1


title: MN_sample2_174_J4_R1


title: MN_sample2_174_I22_R1


title: MN_sample2_174_J3_R1


title: MN_sample2_174_J19_R1


title: MN_sample2_174_J24_R1


title: MN_sample2_174_J13_R1


title: MN_sample2_174_J17_R1


title: MN_sample2_174_I20_R1


title: MN_sample2_174_I24_R1


title: MN_sample2_174_I23_R1


title: MN_sample2_174_I19_R1


title: MN_sample2_174_I14_R1


title: MN_sample2_174_I18_R1


title: MN_sample2_174_I17_R1


title: MN_sample2_174_I15_R1


title: MN_sample2_174_I12_R1


title: MN_sample2_174_I10_R1


title: MN_sample2_174_I13_R1


title: MN_sample2_174_J14_R1


title: MN_sample2_174_I9_R1


title: MN_sample2_174_I6_R1


title: MN_sample2_174_I7_R1


title: MN_sample2_174_I8_R1


title: MN_sample2_174_K8_R1


title: MN_sample2_174_I16_R1


title: MN_sample2_174_I5_R1


title: MN_sample2_174_I21_R1


title: MN_sample2_174_I2_R1


title: MN_sample2_174_I4_R1


title: MN_sample2_174_H23_R1


title: MN_sample2_174_H24_R1


title: MN_sample2_174_H21_R1


title: MN_sample2_174_H22_R1


title: MN_sample2_174_I3_R1


title: MN_sample2_174_H20_R1


title: MN_sample2_174_H18_R1


title: MN_sample2_174_I11_R1


title: MN_sample2_174_H17_R1


title: MN_sample2_174_H14_R1


title: MN_sample2_174_H13_R1


title: MN_sample2_174_H12_R1


title: MN_sample2_174_H15_R1


title: MN_sample2_174_H16_R1


title: MN_sample2_174_H5_R1


title: MN_sample2_174_H7_R1


title: MN_sample2_174_H4_R1


title: MN_sample2_174_H8_R1


title: MN_sample2_174_H10_R1


title: MN_sample2_174_H3_R1


title: MN_sample2_174_I1_R1


title: MN_sample2_174_H2_R1


title: MN_sample2_174_G24_R1


title: MN_sample2_174_G22_R1


title: MN_sample2_174_G21_R1


title: MN_sample2_174_H19_R1


title: MN_sample2_174_G18_R1


title: MN_sample2_174_G16_R1


title: MN_sample2_174_G19_R1


title: MN_sample2_174_H1_R1


title: MN_sample2_174_H6_R1


title: MN_sample2_174_G17_R1


title: MN_sample2_174_G20_R1


title: MN_sample2_174_H11_R1


title: MN_sample2_174_H9_R1


title: MN_sample2_174_G15_R1


title: MN_sample2_174_G14_R1


title: MN_sample2_174_G12_R1


title: MN_sample2_174_G11_R1


title: MN_sample2_174_G10_R1


title: MN_sample2_174_G8_R1


title: MN_sample2_174_G9_R1


title: MN_sample2_174_G6_R1


title: MN_sample2_174_G4_R1


title: MN_sample2_174_G2_R1


title: MN_sample2_174_G3_R1


title: MN_sample2_174_G23_R1


title: MN_sample2_174_G1_R1


title: MN_sample2_174_G5_R1


title: MN_sample2_174_F24_R1


title: MN_sample2_174_F23_R1


title: MN_sample2_174_G13_R1


title: MN_sample2_174_F20_R1


title: MN_sample2_174_F21_R1


title: MN_sample2_174_F17_R1


title: MN_sample2_174_F19_R1


title: MN_sample2_174_F16_R1


title: MN_sample2_174_F13_R1


title: MN_sample2_174_F15_R1


title: MN_sample2_174_F12_R1


title: MN_sample2_174_F14_R1


title: MN_sample2_174_F9_R1


title: MN_sample2_174_F11_R1


title: MN_sample2_174_F7_R1


title: MN_sample2_174_F6_R1


title: MN_sample2_174_F8_R1


title: MN_sample2_174_F5_R1


title: MN_sample2_174_F4_R1


title: MN_sample2_174_F3_R1


title: MN_sample2_174_E24_R1


title: MN_sample2_174_E23_R1


title: MN_sample2_174_F1_R1


title: MN_sample2_174_E22_R1


title: MN_sample2_174_F22_R1


title: MN_sample2_174_F2_R1


title: MN_sample2_174_E20_R1


title: MN_sample2_174_E21_R1


title: MN_sample2_174_E18_R1


title: MN_sample2_174_E17_R1


title: MN_sample2_174_E16_R1


title: MN_sample2_174_G7_R1


title: MN_sample2_174_E19_R1


title: MN_sample2_174_E15_R1


title: MN_sample2_174_E13_R1


title: MN_sample2_174_E12_R1


title: MN_sample2_174_E10_R1


title: MN_sample2_174_E11_R1


title: MN_sample2_174_E9_R1


title: MN_sample2_174_E7_R1


title: MN_sample2_174_E8_R1


title: MN_sample2_174_E14_R1


title: MN_sample2_174_E4_R1


title: MN_sample2_174_E6_R1


title: MN_sample2_174_E3_R1


title: MN_sample2_174_F18_R1


title: MN_sample2_174_D24_R1


title: MN_sample2_174_D22_R1


title: MN_sample2_174_D18_R1


title: MN_sample2_174_E5_R1


title: MN_sample2_174_E1_R1


title: MN_sample2_174_D17_R1


title: MN_sample2_174_E2_R1


title: MN_sample2_174_D20_R1


title: MN_sample2_174_D16_R1


title: MN_sample2_174_D19_R1


title: MN_sample2_174_D13_R1


title: MN_sample2_174_D15_R1


title: MN_sample2_174_F10_R1


title: MN_sample2_174_D9_R1


title: MN_sample2_174_D12_R1


title: MN_sample2_174_D8_R1


title: MN_sample2_174_D10_R1


title: MN_sample2_174_D21_R1


title: MN_sample2_174_D5_R1


title: MN_sample2_174_D14_R1


title: MN_sample2_174_D4_R1


title: MN_sample2_174_D11_R1


title: MN_sample2_174_D6_R1


title: MN_sample2_174_D23_R1


title: MN_sample2_174_D3_R1


title: MN_sample2_174_C21_R1


title: MN_sample2_174_D1_R1


title: MN_sample2_174_C20_R1


title: MN_sample2_174_C14_R1


title: MN_sample2_174_C15_R1


title: MN_sample2_174_C22_R1


title: MN_sample2_174_C23_R1


title: MN_sample2_174_C12_R1


title: MN_sample2_174_D2_R1


title: MN_sample2_174_C18_R1


title: MN_sample2_174_C11_R1


title: MN_sample2_174_C19_R1


title: MN_sample2_174_C17_R1


title: MN_sample2_174_C24_R1


title: MN_sample2_174_C16_R1


title: MN_sample2_174_C8_R1


title: MN_sample2_174_C6_R1


title: MN_sample2_174_C13_R1


title: MN_sample2_174_C5_R1


title: MN_sample2_174_C4_R1


title: MN_sample2_174_C7_R1


title: MN_sample2_174_C9_R1


title: MN_sample2_174_C3_R1


title: MN_sample2_174_B24_R1


title: MN_sample2_174_C2_R1


title: MN_sample2_174_C1_R1


title: MN_sample2_174_B23_R1


title: MN_sample2_174_B22_R1


title: MN_sample2_174_B20_R1


title: MN_sample2_174_C10_R1


title: MN_sample2_174_B21_R1


title: MN_sample2_174_B18_R1


title: MN_sample2_174_B19_R1


title: MN_sample2_174_B16_R1


title: MN_sample2_174_B17_R1


title: MN_sample2_174_B12_R1


title: MN_sample2_174_B14_R1


title: MN_sample2_174_B13_R1


title: MN_sample2_174_B8_R1


title: MN_sample2_174_B11_R1


title: MN_sample2_174_B15_R1


title: MN_sample2_174_B10_R1


title: MN_sample2_174_B5_R1


title: MN_sample2_174_B6_R1


title: MN_sample2_174_B9_R1


title: MN_sample2_174_B4_R1


title: MN_sample2_174_B3_R1


title: MN_sample2_174_B7_R1


title: MN_sample2_174_B2_R1


title: MN_sample2_174_B1_R1


title: MN_sample2_174_A19_R1


title: MN_sample2_174_A23_R1


title: MN_sample2_174_A22_R1


title: MN_sample2_174_A21_R1


title: MN_sample2_174_A20_R1


title: MN_sample2_174_A18_R1


title: MN_sample2_174_A17_R1


title: MN_sample2_174_A16_R1


title: MN_sample2_174_A14_R1


title: MN_sample2_174_A12_R1


title: MN_sample2_174_A11_R1


title: MN_sample2_174_A15_R1


title: MN_sample2_174_A9_R1


title: MN_sample2_174_A8_R1


title: MN_sample2_174_A13_R1


title: MN_sample2_174_A1_R1


title: MN_sample2_174_A5_R1


title: MN_sample1_358_P24_R1


title: MN_sample2_174_A2_R1


title: MN_sample2_174_A3_R1


title: MN_sample1_358_P23_R1


title: MN_sample1_358_P22_R1


title: MN_sample1_358_P20_R1


title: MN_sample1_358_P21_R1


title: MN_sample1_358_P19_R1


title: MN_sample1_358_P17_R1


title: MN_sample1_358_P18_R1


title: MN_sample1_358_P16_R1


title: MN_sample2_174_A6_R1


title: MN_sample1_358_P15_R1


title: MN_sample1_358_P14_R1


title: MN_sample1_358_P12_R1


title: MN_sample2_174_A4_R1


title: MN_sample2_174_A7_R1


title: MN_sample1_358_P11_R1


title: MN_sample1_358_P9_R1


title: MN_sample1_358_P10_R1


title: MN_sample2_174_A10_R1


title: MN_sample1_358_P8_R1


title: MN_sample1_358_P5_R1


title: MN_sample1_358_P4_R1


title: MN_sample1_358_P6_R1


title: MN_sample1_358_P7_R1


title: MN_sample1_358_P2_R1


title: MN_sample1_358_P3_R1


title: MN_sample1_358_P1_R1


title: MN_sample1_358_O22_R1


title: MN_sample1_358_O21_R1


title: MN_sample1_358_O23_R1


title: MN_sample1_358_O20_R1


title: MN_sample1_358_O16_R1


title: MN_sample1_358_O19_R1


title: MN_sample1_358_P13_R1


title: MN_sample1_358_O17_R1


title: MN_sample1_358_O15_R1


title: MN_sample1_358_O14_R1


title: MN_sample1_358_O13_R1


title: MN_sample1_358_O12_R1


title: MN_sample1_358_O10_R1


title: MN_sample1_358_O11_R1


title: MN_sample1_358_O8_R1


title: MN_sample1_358_O9_R1


title: MN_sample1_358_O24_R1


title: MN_sample1_358_O6_R1


title: MN_sample1_358_O5_R1


title: MN_sample1_358_O1_R1


title: MN_sample1_358_N23_R1


title: MN_sample1_358_N24_R1


title: MN_sample1_358_N22_R1


title: MN_sample1_358_O18_R1


title: MN_sample1_358_N20_R1


title: MN_sample1_358_N19_R1


title: MN_sample1_358_N18_R1


title: MN_sample1_358_N15_R1


title: MN_sample2_174_A24_R1


title: MN_sample1_358_N17_R1


title: MN_sample1_358_N16_R1


title: MN_sample1_358_N13_R1


title: MN_sample1_358_N14_R1


title: MN_sample1_358_N11_R1


title: MN_sample1_358_N12_R1


title: MN_sample1_358_O7_R1


title: MN_sample1_358_N8_R1


title: MN_sample1_358_N10_R1


title: MN_sample1_358_N9_R1


title: MN_sample1_358_O3_R1


title: MN_sample1_358_N7_R1


title: MN_sample1_358_O2_R1


title: MN_sample1_358_O4_R1


title: MN_sample1_358_N5_R1


title: MN_sample1_358_N4_R1


title: MN_sample1_358_N2_R1


title: MN_sample1_358_N1_R1


title: MN_sample1_358_M23_R1


title: MN_sample1_358_N21_R1


title: MN_sample1_358_M24_R1


title: MN_sample1_358_M22_R1


title: MN_sample1_358_M21_R1


title: MN_sample1_358_M19_R1


title: MN_sample1_358_M17_R1


title: MN_sample1_358_M18_R1


title: MN_sample1_358_M15_R1


title: MN_sample1_358_M16_R1


title: MN_sample1_358_M14_R1


title: MN_sample1_358_M10_R1


title: MN_sample1_358_N6_R1


title: MN_sample1_358_M8_R1


title: MN_sample1_358_M7_R1


title: MN_sample1_358_M9_R1


title: MN_sample1_358_M6_R1


title: MN_sample1_358_M5_R1


title: MN_sample1_358_N3_R1


title: MN_sample1_358_M2_R1


title: MN_sample1_358_M3_R1


title: MN_sample1_358_M1_R1


title: MN_sample2_174_D7_R1


title: MN_sample1_358_L19_R1


title: MN_sample1_358_L23_R1


title: MN_sample1_358_L21_R1


title: MN_sample1_358_L18_R1


title: MN_sample1_358_L17_R1


title: MN_sample1_358_M11_R1


title: MN_sample1_358_M12_R1


title: MN_sample1_358_L16_R1


title: MN_sample1_358_L15_R1


title: MN_sample1_358_L12_R1


title: MN_sample1_358_L14_R1


title: MN_sample1_358_M13_R1


title: MN_sample1_358_L10_R1


title: MN_sample1_358_L11_R1


title: MN_sample1_358_L8_R1


title: MN_sample1_358_L9_R1


title: MN_sample1_358_L6_R1


title: MN_sample1_358_L7_R1


title: MN_sample1_358_L4_R1


title: MN_sample1_358_L24_R1


title: MN_sample1_358_L2_R1


title: MN_sample1_358_L22_R1


title: MN_sample1_358_L20_R1


title: MN_sample1_358_L3_R1


title: MN_sample1_358_L1_R1


title: MN_sample1_358_K23_R1


title: MN_sample1_358_L5_R1


title: MN_sample1_358_K24_R1


title: MN_sample1_358_K21_R1


title: MN_sample1_358_L13_R1


title: MN_sample1_358_K16_R1


title: MN_sample1_358_K12_R1


title: MN_sample1_358_K18_R1


title: MN_sample1_358_K9_R1


title: MN_sample1_358_K6_R1


title: MN_sample1_358_K13_R1


title: MN_sample1_358_K15_R1


title: MN_sample1_358_K11_R1


title: MN_sample1_358_K10_R1


title: MN_sample1_358_K3_R1


title: MN_sample1_358_K5_R1


title: MN_sample1_358_K2_R1


title: MN_sample1_358_J23_R1


title: MN_sample1_358_K4_R1


title: MN_sample1_358_K22_R1


title: MN_sample1_358_K20_R1


title: MN_sample1_358_K17_R1


title: MN_sample1_358_K7_R1


title: MN_sample1_358_J22_R1


title: MN_sample1_358_J20_R1


title: MN_sample1_358_K1_R1


title: MN_sample1_358_J24_R1


title: MN_sample1_358_M4_R1


title: MN_sample1_358_J21_R1


title: MN_sample1_358_J19_R1


title: MN_sample1_358_K8_R1


title: MN_sample1_358_J17_R1


title: MN_sample1_358_K19_R1


title: MN_sample1_358_J18_R1


title: MN_sample1_358_K14_R1


title: MN_sample1_358_J16_R1


title: MN_sample1_358_J15_R1


title: MN_sample1_358_J13_R1


title: MN_sample1_358_J11_R1


title: MN_sample1_358_J14_R1


title: MN_sample1_358_J10_R1


title: MN_sample1_358_J9_R1


title: MN_sample1_358_J8_R1


title: MN_sample1_358_M20_R1


title: MN_sample1_358_I21_R1


title: MN_sample1_358_I20_R1


title: MN_sample1_358_J6_R1


title: MN_sample1_358_J2_R1


title: MN_sample1_358_J7_R1


title: MN_sample1_358_I22_R1


title: MN_sample1_358_J5_R1


title: MN_sample1_358_J1_R1


title: MN_sample1_358_J4_R1


title: MN_sample1_358_I23_R1


title: MN_sample1_358_J3_R1


title: MN_sample1_358_I24_R1


title: MN_sample1_358_I19_R1


title: MN_sample1_358_I17_R1


title: MN_sample1_358_I16_R1


title: MN_sample1_358_I15_R1


title: MN_sample1_358_I14_R1


title: MN_sample1_358_I13_R1


title: MN_sample1_358_I11_R1


title: MN_sample1_358_I10_R1


title: MN_sample1_358_J12_R1


title: MN_sample1_358_I12_R1


title: MN_sample1_358_I9_R1


title: MN_sample1_358_I8_R1


title: MN_sample1_358_I7_R1


title: MN_sample1_358_I6_R1


title: MN_sample1_358_I5_R1


title: MN_sample1_358_I4_R1


title: MN_sample1_358_I3_R1


title: MN_sample1_358_I18_R1


title: MN_sample1_358_I1_R1


title: MN_sample1_358_I2_R1


title: MN_sample1_358_H17_R1


title: MN_sample1_358_H20_R1


title: MN_sample1_358_H19_R1


title: MN_sample1_358_H24_R1


title: MN_sample1_358_H21_R1


title: MN_sample1_358_H15_R1


title: MN_sample1_358_H22_R1


title: MN_sample1_358_H14_R1


title: MN_sample1_358_H12_R1


title: MN_sample1_358_H16_R1


title: MN_sample1_358_H10_R1


title: MN_sample1_358_H13_R1


title: MN_sample1_358_H9_R1


title: MN_sample1_358_H18_R1


title: MN_sample1_358_H11_R1


title: MN_sample1_358_H8_R1


title: MN_sample1_358_H6_R1


title: MN_sample1_358_H7_R1


title: MN_sample1_358_H5_R1


title: MN_sample1_358_H2_R1


title: MN_sample1_358_H4_R1


title: MN_sample1_358_G24_R1

description: missing raw data are due to file corruption


title: MN_sample1_358_H3_R1