title: Jinjudaepyung_V40-3

description: replicate 3


title: TNBC 1

status: Public on Nov 01 2023

type: RNA

city: Guangzhou

first: Huang

middle: Shan

last: Shan

address: 651 Dongfeng Road East

country: China

institute: Sun Yat-sen University Cancer Center

description: PiRNA expression profiles in triple negative breast cancer

hyb_protocol: 0.6 μg of Cy3-labelled cRNA (specific activity >10.0 pmol Cy3/ug cRNA) was fragmented at 60°C for 30 minutes in a reaction volume of 22.5μl containing 1x Agllent fragmentation buffer and 2x Agilent blocking agent following the manufacturers'instructions. On completian of the fragmentation reaction, 22.5μl of 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to Arraystar Human piRNA Array in Agilent's SureHyb Hybridization Chambers for 17 hours at 65C in a rotating Agilent hybridization oven.After hybridization,microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agllent) and 1 minute with 37°C GE Wash buffer 2 (Agilent), then dried immediately by brief centrifugation.

scan_protocol: Slides were scanned immediately after washing on the Agllent DNA Microarray Scanner (G2505C) using one color scan setting for 4x180k array slides (Scan Area 61x21.6 mm, Scan resolution 3um, Dye channel is set to Green and Green PMT is set data processing to 100%).

data_processing: The scanned images were analyzed with Feature Extraction Software 10.7.1.1 (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities as the raw data. Raw data were normalized in quantile algorithm with Genespring 13.0 (Agilent). Probe that at least 1 out of 2 samples flagged as Detectedwere maintained.

molecule: total RNA

organism: Homo sapiens

source_name: Triple negative breast tissue

label_protocol: Cyanine-3 (Cy3) labeled cRNA was prepared from 0.2 μg RNA using the One-Color Low RNA Input Linear Amplification PLUS kit (Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN,Valencia,CA). Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.

extract_protocol: RNA was extracted by mirVanaTM RNA Isolation Kit (Applied Biosystem p/n AM1556) following the manufacture's instructions

tag: tissue

value: breast tissue

tag: gender

value: female


title: Jinjudaepyung_AV-1

type: SRA

city: Anseong-si,

first: Sang Woo

last: Lee

address: 4726 Seodong-daero, Daedeok-myeon

department: Plant Science and Technology

country: South Korea

institute: CHUNG-ANG UNIVERSITY

description: replicate 1

library_source: transcriptomic

data_processing:
Quality of the RNA-seq reads were evaluated using the FastQC software.
The raw reads were trimmed and quality-filtered before genome alignment using Trimmomatic (ver0.36) with more than 90% portion (Q>30) were only used in the mapping.
Reference genome of Brassica rapa plants was obtained from Ensembl (https://plants.ensembl.org/info/website/ftp/index.html) and mapped using TopHat2 software with default parameters.
Aligned reads were converted to digital counts using FeatureCounts and analyzed using edgeR.
Differentially expressed genes (DEGs) were identified based on a p-value < 0.05 and two-fold difference cut-off.
Assembly: Raphanus sativus L. reference genome was obtained from Radish Genome Database (http://radish-genome.org/)
Supplementary files format and content: tab-delimited text files with raw gene counts for individual samples

organism: Raphanus sativus

source_name: whole plant

extract_protocol:
Total RNA was extracted from whole seedling samples using RNeasy Mini Kit (Qiagen, Germany) and treated with DNase I (New England Biolabs, USA) to remove genomic DNA contamination.
RNA-seq libraries were constructed using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina Inc., USA) according to the manufacturer’s instructions.

value: whole plant

tag: line

value: Jinjudaepyung

tag: genotype

value: Wild type

tag: treatment

value: AV


title: Spleen, Survivor, Day 0 (Fp_36_S)

city: Frederiksberg

first: Per

middle: Walter

last: Kania

address: Stigbojlen 7

department: Department of Veterinary and Animal Sciences

country: Denmark

institute: University of Copenhagen

data_processing:
All q-PCR assays exhibited efficiencies within 100% ± 5% so the simplified 2-ΔΔCq method was used for analysing data (Livak and Schmittgen, 2001). For normalization and as internal calibrator the average of three genes (ARP, ELF1α and β-actin) was chosen by using NormFinder (Andersen et al., 2004). For gene expression analyses, all challenged groups were compared to non-exposed time point controls using Student’s t-test. We considered differences to be significant when regulations were at least two-fold and p < 0.05. For four of the genes investigated in the non-exposed timepoint control groups less than three fish showed Cq values (excluding a t-test) and therefore we applied a qualitative assessment (presence/absence of Cq values) and analyzed data with the nonparametric Mann-Whitney test using a probability level of 5%.
Target gene signals were normalized using the average of 3 housekeeping genes (ARP, b-actin and ELF 1a) as2^-ΔCt, where -ΔCt = -(Ct_Target − Ct_ average of 3 HKGs). Folds were calculated as  2^-ΔΔCt, where -ΔΔCt = -[ΔCtinfected -ΔCtUninfected] using idividual uninfected time point controls. No fold changes was calculated for samples timepoint zero since there were no infected group at that timepoint.

label: FAM

organism: Oncorhynchus mykiss

source_name: Survivor

label_protocol: cDNA synthesis was performed in T100 thermocycler, Biorad, Denmark, using Oligo d(T)16 primer and TaqMan® Reverse Transcription Reagents (cat.no. N8080234, Thermo Fischer Scientific, Denmark). Finally, cDNA was stored at −20°C until further use. Quantitative PCR assays were performed using an AriaMx Real-Time PCR machine (cat.no.G8830A-04R-010, AH diagnostics AS, Denmark). The cycling conditions were one cycle of predenaturation at 95°C for 3 min. This was followed by 40 cycles of denaturation at 94°C for 5 sec with a combined annealing/elongation process at 60°C for 15 sec with endpoint measurement. Primers and Taq-Man probes targeting immune-relevant rainbow trout genes (total of 28 genes including three housekeeping genes) were synthesized at TAG Copenhagen AS, Denmark. Reaction volumes were 12.5 μl (2.5 μl cDNA, 6.25 μl Brilliant III Ultra-Fast QPCR Master Mix (600881, AH Diagnostics AS, Denmark), 1.0 μl primer-probe mixture (forward primer, 10 μM and reverse primer, 10 μM), Taq-Man probe (5 μM), and 2.75 μl RNase-free water. Reverse transcriptase minus and negative controls were used for every plate setup. . NormFinder (Andersen et al., 2004) was applied to evaluate all combinations of three reference genes (Elongation factor (ELF) 1-α, β-actin and acidic ribosomal phosphoprotein P0 (ARP)) as endogenous control; the average of the three genes was chosen and stability values were 0.002, 0.005 and 0.002 for liver, spleen and gills, respectively. In order to quantify the bacterial load in different organs at the different time points we measured the gene expression level of the bacterial gene recA, which was described as a household gene in V.anguillarum  (GenBank ass.no. LC370212) by Crisafi et al. (2014). We designed the probe for the qPCR assay according to Zuo et al. (2020) with minor modifications. For efficiency determination, estimated 102%, dilution series of samples with high expression of V. anguillarum recA was used. Specificity was tested by SYBER green and melting curve analysis confirmed by electrophoresis, product length 248 bp. Bacterial transcript level was estimated as 2-ΔCq . It should be noted that dead or inactive bacteria may not be detected by this method.

growth_protocol: The fish were kept in separate aerated INTEX® plastic tanks in two different rooms (one room for infected fish, one room for non-infected controls). The tank dimensions were (2.6 m x 1.6 m x 0.65 m, total volume 2.7 m3) and each tank contained 800 L of municipal water. Internal biofilters (20 L min-1 EHEIM, Germany) were placed in both tanks to maintain water quality (pH 7.6, nitrite <0.01 mg/L, nitrate <50 mg/L (TetraTest 6in1, cat.no. T704154, Eldorado A/S, Denmark), ammonia <0.5 mg/l (API Ammonia Test Strips, VioVet Ltd., United Kingdom)). Temperature was kept at 11-12°C, and the light/dark cycle was 12h light /12h dark. The fish received commercial pelleted dry feed (1% biomass daily) (INICIO 917, BioMar A/S, Brande, Denmark).

extract_protocol: RNAlater fixed samples from fish (gills, liver and spleen) were homogenized (Tissue-lyser II, Qiagen, Denmark) using a homogenization buffer with 2-mercaptoethanol (Sigma-Aldrich). RNA was extracted using the GenEluteTM mammalian RNA kit (RTN350, Sigma-Aldrich, Denmark). Liver samples were pre-treated by Proteinase K (cat.no.P4850, Sigma-Aldrich, Denmark). DNase I (AMPD1, Sigma-Aldrich, Denmark) treatment was applied to remove genomic DNA. The concentration of RNA was determined in a NanoDrop 2000 spectrophotometer (Saveen & Werner, Denmark). Quality of RNA was evaluated by agarose (cat.no. 16500100, ThermoFisher Scientific, Denmark) gel electrophoresis. RNA was kept at −80°C until cDNA synthesis.

treatment_protocol: Tissue (liver, spleen, gills) samples were fixed in RNAlater (cat.no R0901, Sigma-Aldrich, Denmark), placed at 4°C for 24 h and subsequently stored at -20°C until further processing.

tag: replicate

value: Replica 6

value: Spleen

tag: infection

value: Flavobacterium psychrophilum

tag: time

value: Day 40

tag: outcome

value: Survivor


title: Jinjudaepyung_NV-1

description: replicate 1

value: NV


title: Spleen, Survivor, Day 0 (Fp_38_S)


title: Jinjudaepyung_NV-3

description: replicate 3


title: Spleen, Survivor, Day 0 (Fp_40_S)


title: Jinjudaepyung_V40-2

description: replicate 2


title: Jinjudaepyung_NV-2

description: replicate 2


title: Spleen, Survivor, Day 0 (Fp_33_S)


title: Spleen, Survivor, Day 0 (Fp_28_S)


title: Spleen, Survivor, Day 0 (Fp_31_S)


title: Jinjudaepyung_V40-1

description: replicate 1


title: Spleen, Survivor, Day 0 (Fp_32_S)


title: Spleen, Survivor, Day 0 (Fp_37_S)


title: Spleen, Survivor, Day 0 (Fp_24_S)


title: Spleen, Survivor, Day 0 (Fp_25_S)


title: Spleen, Survivor, Day 0 (Fp_29_S)


title: Spleen, Control, Day 40 (Fp_C57_S)

source_name: Time point Control

value: Replica 5

value: Uninfected

value: Time point Control


title: Spleen, Control, Day 40 (Fp_C58_S)


title: Spleen, Control, Day 40 (Fp_C60_S)


title: Spleen, Survivor, Day 0 (Fp_21_S)


title: Spleen, Control, Day 40 (Fp_C56_S)


title: Spleen, Survivor, Day 0 (Fp_22_S)


title: Spleen, Control, Day 40 (Fp_C55_S)


title: Spleen, Control, Day 40 (Fp_C53_S)


title: Spleen, Control, Day 40 (Fp_C52_S)


title: Spleen, Survivor, Day 0 (Fp_34_S)


title: Spleen, Survivor, Day 0 (Fp_26_S)


title: Spleen, Control, Day 40 (Fp_C51_S)


title: Spleen, Control, Day 40 (Fp_C50_S)


title: Spleen, Control, Day 40 (Fp_C49_S)


title: Spleen, Control, Day 40 (Fp_C46_S)


title: Spleen, Control, Day 40 (Fp_C45_S)


title: Spleen, Control, Day 40 (Fp_C42_S)


title: Spleen, Control, Day 40 (Fp_C41_S)


title: Spleen,  Clinical Sign, Day 11 to 14 (Fp_20_S)

source_name: Clinical Sign

value: Replica 4

value: Day 11 to 14

value: Clinical Sign


title: Spleen, Control, Day 40 (Fp_C43_S)


title: Spleen,  Clinical Sign, Day 11 to 14 (Fp_19_S)


title: Spleen,  Clinical Sign, Day 11 to 14 (Fp_14_S)


title: Spleen,  Clinical Sign, Day 11 to 14 (Fp_9_S)


title: Spleen,  Clinical Sign, Day 11 to 14 (Fp_18_S)


title: Spleen,  Clinical Sign, Day 11 to 14 (Fp_11_S)


title: Spleen,  Clinical Sign, Day 11 to 14 (Fp_15_S)


title: Spleen,  Clinical Sign, Day 11 to 14 (Fp_17_S)


title: Spleen,  Clinical Sign, Day 11 to 14 (Fp_8_S)


title: Spleen,  Clinical Sign, Day 11 to 14 (Fp_5_S)


title: Spleen, No Clinical Sign, Day 11 to 14 (Fp_T14-8_S)

source_name: No Clinical Sign

value: Replica 3

value: No Clinical Sign


title: Spleen,  Clinical Sign, Day 11 to 14 (Fp_10_S)


title: Spleen, No Clinical Sign, Day 11 to 14 (Fp_T14-7_S)


title: Spleen, No Clinical Sign, Day 11 to 14 (Fp_T14-10_S)


title: Spleen,  Clinical Sign, Day 11 to 14 (Fp_13_S)


title: Spleen,  Clinical Sign, Day 11 to 14 (Fp_3_S)


title: Spleen,  Clinical Sign, Day 11 to 14 (Fp_6_S)


title: Spleen,  Clinical Sign, Day 11 to 14 (Fp_4_S)


title: Spleen, No Clinical Sign, Day 11 to 14 (Fp_T14-6_S)


title: Spleen, No Clinical Sign, Day 11 to 14 (Fp_T14-4_S)


title: Spleen, No Clinical Sign, Day 11 to 14 (Fp_T14-3_S)


title: Spleen, No Clinical Sign, Day 11 to 14 (Fp_T14-2_S)


title: Spleen, No Clinical Sign, Day 11 to 14 (Fp_T13-10_S)


title: Spleen, No Clinical Sign, Day 11 to 14 (Fp_T14-1_S)


title: Spleen, No Clinical Sign, Day 11 to 14 (Fp_T13-6_S)


title: Spleen, No Clinical Sign, Day 11 to 14 (Fp_T13-4_S)


title: Spleen, No Clinical Sign, Day 11 to 14 (Fp_T13-1_S)


title: Spleen, No Clinical Sign, Day 11 to 14 (Fp_T13-3_S)


title: Spleen, No Clinical Sign, Day 11 to 14 (Fp_T13-2_S)


title: Spleen, Control, Day 11 to 14 (Fp_C39_S)

value: Replica 2


title: Spleen, Control, Day 11 to 14 (Fp_C40_S)


title: Spleen, Control, Day 11 to 14 (Fp_C37_S)


title: Spleen, Control, Day 11 to 14 (Fp_C30_S)


title: Spleen, Control, Day 11 to 14 (Fp_C29_S)


title: Spleen, Control, Day 11 to 14 (Fp_C32_S)


title: Spleen, Control, Day 11 to 14 (Fp_C36_S)


title: Spleen, Control, Day 11 to 14 (Fp_C35_S)


title: Spleen, Control, Day 11 to 14 (Fp_C28_S)


title: Spleen, Control, Day 11 to 14 (Fp_C33_S)


title: Spleen, Control, Day 11 to 14 (Fp_C27_S)


title: Spleen, Control, Day 0 (Fp_C15_S)

value: Replica 1

value: Day 0


title: Spleen, Control, Day 11 to 14 (Fp_C25_S)


title: Spleen, Control, Day 0 (Fp_C19_S)


title: Spleen, Control, Day 0 (Fp_C20_S)


title: Spleen, Control, Day 0 (Fp_C17_S)


title: Spleen, Control, Day 11 to 14 (Fp_C24_S)


title: Spleen, Control, Day 0 (Fp_C14_S)


title: Spleen, Control, Day 11 to 14 (Fp_C23_S)


title: Spleen, Control, Day 0 (Fp_C12_S)


title: Spleen, No Clinical Sign, Day 11 to 14 (Fp_T13-9_S)


title: Spleen, Control, Day 0 (Fp_C11_S)


title: Spleen, Control, Day 0 (Fp_C10_S)


title: Spleen, Control, Day 11 to 14 (Fp_C38_S)


title: Spleen, Control, Day 0 (Fp_C7_S)


title: Spleen, Control, Day 0 (Fp_C5_S)


title: Spleen, Control, Day 0 (Fp_C4_S)


title: Liver, Survivor, Day 0 (Fp_39_L)

value: Replica 6

value: Liver


title: Spleen, Control, Day 0 (Fp_C2_S)

value: Spleen


title: Spleen, Control, Day 0 (Fp_C1_S)


title: Liver, Survivor, Day 0 (Fp_36_L)

value: Flavobacterium psychrophilum

value: Day 40


title: Liver, Survivor, Day 0 (Fp_35_L)

value: Survivor


title: Liver, Survivor, Day 0 (Fp_34_L)


title: Liver, Survivor, Day 0 (Fp_32_L)


title: Liver, Survivor, Day 0 (Fp_33_L)


title: Liver, Survivor, Day 0 (Fp_30_L)


title: Liver, Survivor, Day 0 (Fp_27_L)


title: Liver, Survivor, Day 0 (Fp_28_L)


title: Liver, Survivor, Day 0 (Fp_29_L)


title: Liver, Survivor, Day 0 (Fp_25_L)


title: Liver, Survivor, Day 0 (Fp_22_L)


title: Liver, Survivor, Day 0 (Fp_23_L)


title: Liver, Control, Day 40 (Fp_C60_L)

value: Replica 5


title: Spleen, Control, Day 0 (Fp_C9_S)

value: Uninfected


title: Liver, Survivor, Day 0 (Fp_26_L)


title: Liver, Control, Day 40 (Fp_C58_L)

value: Time point Control


title: Liver, Control, Day 40 (Fp_C59_L)


title: Liver, Control, Day 40 (Fp_C55_L)


title: Liver, Control, Day 40 (Fp_C56_L)


title: Spleen, Control, Day 0 (Fp_C6_S)


title: Liver, Control, Day 40 (Fp_C57_L)


title: Liver, Control, Day 40 (Fp_C54_L)


title: Liver, Control, Day 40 (Fp_C53_L)


title: Liver, Control, Day 40 (Fp_C49_L)


title: Liver, Control, Day 40 (Fp_C48_L)


title: Liver, Control, Day 40 (Fp_C47_L)


title: Liver, Control, Day 40 (Fp_C46_L)


title: Liver,  Clinical Sign, Day 11 to 14 (Fp_20_L)

value: Replica 4


title: Liver, Control, Day 40 (Fp_C44_L)


title: Liver, Survivor, Day 0 (Fp_21_L)


title: Liver,  Clinical Sign, Day 11 to 14 (Fp_16_L)

value: Day 11 to 14


title: Liver,  Clinical Sign, Day 11 to 14 (Fp_14_L)

value: Clinical Sign


title: Liver,  Clinical Sign, Day 11 to 14 (Fp_15_L)


title: Liver,  Clinical Sign, Day 11 to 14 (Fp_13_L)


title: Liver,  Clinical Sign, Day 11 to 14 (Fp_8_L)


title: Liver,  Clinical Sign, Day 11 to 14 (Fp_7_L)


title: Liver,  Clinical Sign, Day 11 to 14 (Fp_9_L)


title: Liver, No Clinical Sign, Day 11 to 14 (Fp_T143_L)

value: Replica 3


title: Liver, No Clinical Sign, Day 11 to 14 (Fp_T1410_L)

value: No Clinical Sign


title: Liver,  Clinical Sign, Day 11 to 14 (Fp_2_L)


title: Liver, No Clinical Sign, Day 11 to 14 (Fp_T146_L)


title: Liver,  Clinical Sign, Day 11 to 14 (Fp_3_L)


title: Liver,  Clinical Sign, Day 11 to 14 (Fp_4_L)


title: Liver,  Clinical Sign, Day 11 to 14 (Fp_5_L)


title: Liver, Control, Day 40 (Fp_C52_L)


title: Liver, No Clinical Sign, Day 11 to 14 (Fp_T144_L)


title: Liver, No Clinical Sign, Day 11 to 14 (Fp_T1310_L)


title: Liver, No Clinical Sign, Day 11 to 14 (Fp_T148_L)


title: Liver, No Clinical Sign, Day 11 to 14 (Fp_T139_L)


title: Liver, No Clinical Sign, Day 11 to 14 (Fp_T137_L)


title: Liver, Control, Day 40 (Fp_C45_L)


title: Liver, No Clinical Sign, Day 11 to 14 (Fp_T138_L)


title: Liver,  Clinical Sign, Day 11 to 14 (Fp_18_L)


title: Liver, No Clinical Sign, Day 11 to 14 (Fp_T136_L)


title: Liver,  Clinical Sign, Day 11 to 14 (Fp_19_L)


title: Liver, No Clinical Sign, Day 11 to 14 (Fp_T134_L)


title: Liver, No Clinical Sign, Day 11 to 14 (Fp_T132_L)


title: Liver, No Clinical Sign, Day 11 to 14 (Fp_T131_L)


title: Liver, Control, Day 11 to 14 (Fp_C37_L)

value: Replica 2


title: Liver, Control, Day 11 to 14 (Fp_C40_L)


title: Liver,  Clinical Sign, Day 11 to 14 (Fp_11_L)


title: Liver, Control, Day 11 to 14 (Fp_C35_L)


title: Liver, Control, Day 11 to 14 (Fp_C25_L)


title: Liver, Control, Day 11 to 14 (Fp_C34_L)


title: Liver, Control, Day 11 to 14 (Fp_C24_L)


title: Liver, Control, Day 11 to 14 (Fp_C26_L)


title: Liver, Control, Day 11 to 14 (Fp_C31_L)


title: Liver, Control, Day 11 to 14 (Fp_C27_L)


title: Liver, Control, Day 11 to 14 (Fp_C29_L)


title: Liver, Control, Day 11 to 14 (Fp_C22_L)


title: Liver, Control, Day 11 to 14 (Fp_C32_L)


title: Liver, No Clinical Sign, Day 11 to 14 (Fp_T135_L)


title: Liver, Control, Day 0 (Fp_C20_L)

value: Replica 1


title: Liver, No Clinical Sign, Day 11 to 14 (Fp_T133_L)


title: Liver, Control, Day 0 (Fp_C18_L)

value: Day 0


title: Liver, Control, Day 0 (Fp_C19_L)


title: Liver, Control, Day 0 (Fp_C11_L)


title: Liver, Control, Day 0 (Fp_C10_L)


title: Liver, Control, Day 0 (Fp_C9_L)


title: Liver, Control, Day 0 (Fp_C13_L)


title: Liver, Control, Day 0 (Fp_C8_L)


title: Liver, Control, Day 0 (Fp_C5_L)


title: Liver, Control, Day 0 (Fp_C6_L)


title: Liver, Control, Day 11 to 14 (Fp_C36_L)


title: Liver, Control, Day 0 (Fp_C3_L)


title: Liver, Control, Day 11 to 14 (Fp_C33_L)


title: Liver, Control, Day 0 (Fp_C2_L)


title: Liver, Control, Day 11 to 14 (Fp_C30_L)


title: Liver, Control, Day 0 (Fp_C1_L)


title: Gill, Survivor, Day 0 (Fp_34_G)

value: Replica 6

value: Gill


title: Gill, Survivor, Day 0 (Fp_38_G)


title: Liver, Control, Day 0 (Fp_C16_L)

value: Liver


title: Gill, Survivor, Day 0 (Fp_33_G)

value: Flavobacterium psychrophilum


title: Gill, Survivor, Day 0 (Fp_30_G)

value: Day 40


title: Gill, Survivor, Day 0 (Fp_36_G)

value: Survivor


title: Gill, Survivor, Day 0 (Fp_35_G)


title: Gill, Survivor, Day 0 (Fp_37_G)


title: Gill, Survivor, Day 0 (Fp_32_G)


title: Gill, Survivor, Day 0 (Fp_21_G)


title: Gill, Survivor, Day 0 (Fp_29_G)


title: Liver, Control, Day 0 (Fp_C4_L)


title: Gill, Survivor, Day 0 (Fp_24_G)


title: Gill, Survivor, Day 0 (Fp_31_G)


title: Gill, Survivor, Day 0 (Fp_27_G)


title: Gill, Survivor, Day 0 (Fp_26_G)


title: Gill, Control, Day 40 (Fp_C52_G)

value: Replica 5


title: Gill, Control, Day 40 (Fp_C55_G)

value: Uninfected


title: Gill, Control, Day 40 (Fp_C50_G)

value: Time point Control


title: Gill, Control, Day 40 (Fp_C58_G)


title: Gill, Control, Day 40 (Fp_C51_G)


title: Gill, Control, Day 40 (Fp_C49_G)


title: Gill, Control, Day 40 (Fp_C46_G)


title: Gill, Control, Day 40 (Fp_C47_G)


title: Gill, Survivor, Day 0 (Fp_40_G)


title: Gill, Control, Day 40 (Fp_C45_G)


title: Gill, Control, Day 40 (Fp_C44_G)


title: Gill, Control, Day 40 (Fp_C57_G)


title: Gill, Control, Day 40 (Fp_C41_G)


title: Gill, Control, Day 40 (Fp_C59_G)


title: Gill, Control, Day 40 (Fp_C42_G)


title: Gill,  Clinical Sign, Day 11 to 14 (Fp_14_G)

value: Replica 4


title: Gill,  Clinical Sign, Day 11 to 14 (Fp_17_G)

value: Day 11 to 14


title: Gill,  Clinical Sign, Day 11 to 14 (Fp_19_G)

value: Clinical Sign


title: Gill,  Clinical Sign, Day 11 to 14 (Fp_16_G)


title: Gill,  Clinical Sign, Day 11 to 14 (Fp_11_G)


title: Gill,  Clinical Sign, Day 11 to 14 (Fp_13_G)


title: Gill,  Clinical Sign, Day 11 to 14 (Fp_9_G)


title: Gill, Control, Day 40 (Fp_C48_G)


title: Gill,  Clinical Sign, Day 11 to 14 (Fp_6_G)


title: Gill,  Clinical Sign, Day 11 to 14 (Fp_1_G)


title: Gill,  Clinical Sign, Day 11 to 14 (Fp_7_G)


title: Gill,  Clinical Sign, Day 11 to 14 (Fp_8_G)


title: Gill,  Clinical Sign, Day 11 to 14 (Fp_3_G)


title: Gill,  Clinical Sign, Day 11 to 14 (Fp_5_G)


title: Gill,  Clinical Sign, Day 11 to 14 (Fp_2_G)


title: Gill, No Clinical Sign, Day 11 to 14 (Fp_T146_G)

value: Replica 3


title: Gill, No Clinical Sign, Day 11 to 14 (Fp_T1410_G)

value: No Clinical Sign


title: Gill,  Clinical Sign, Day 11 to 14 (Fp_4_G)


title: Gill, No Clinical Sign, Day 11 to 14 (Fp_T147_G)


title: Gill, No Clinical Sign, Day 11 to 14 (Fp_T148_G)


title: Gill, No Clinical Sign, Day 11 to 14 (Fp_T145_G)


title: Gill, No Clinical Sign, Day 11 to 14 (Fp_T144_G)


title: Gill, No Clinical Sign, Day 11 to 14 (Fp_T143_G)


title: Gill, No Clinical Sign, Day 11 to 14 (Fp_T1310_G)


title: Gill, No Clinical Sign, Day 11 to 14 (Fp_T139_G)


title: Gill, No Clinical Sign, Day 11 to 14 (Fp_T142_G)


title: Gill, No Clinical Sign, Day 11 to 14 (Fp_T134_G)


title: Gill, No Clinical Sign, Day 11 to 14 (Fp_T135_G)


title: Gill, No Clinical Sign, Day 11 to 14 (Fp_T132_G)


title: Gill, Control, Day 11 to 14 (Fp_C39_G)

value: Replica 2


title: Gill, Control, Day 11 to 14 (Fp_C38_G)


title: Gill, Control, Day 11 to 14 (Fp_C37_G)


title: Gill, No Clinical Sign, Day 11 to 14 (Fp_T133_G)


title: Gill, Control, Day 11 to 14 (Fp_C30_G)


title: Gill, Control, Day 11 to 14 (Fp_C31_G)


title: Gill, Control, Day 11 to 14 (Fp_C33_G)


title: Gill, Control, Day 11 to 14 (Fp_C35_G)


title: Gill, No Clinical Sign, Day 11 to 14 (Fp_T138_G)


title: Gill, Control, Day 11 to 14 (Fp_C23_G)


title: Gill, Control, Day 11 to 14 (Fp_C34_G)


title: Gill, Control, Day 11 to 14 (Fp_C25_G)


title: Gill, Control, Day 11 to 14 (Fp_C26_G)


title: Gill, Control, Day 0 (Fp_C20_G)

value: Replica 1


title: Gill, Control, Day 11 to 14 (Fp_C32_G)


title: Gill, Control, Day 11 to 14 (Fp_C21_G)


title: Gill, Control, Day 0 (Fp_C18_G)

value: Day 0


title: Gill, Control, Day 0 (Fp_C16_G)


title: Gill, Control, Day 11 to 14 (Fp_C27_G)


title: Gill, Control, Day 0 (Fp_C19_G)


title: Gill, Control, Day 0 (Fp_C14_G)


title: Gill, Control, Day 0 (Fp_C13_G)


title: Gill, Control, Day 0 (Fp_C12_G)


title: Gill, Control, Day 11 to 14 (Fp_C36_G)


title: Gill, Control, Day 0 (Fp_C11_G)


title: Gill, Control, Day 0 (Fp_C10_G)


title: Gill, Control, Day 0 (Fp_C9_G)


title: Gill, Control, Day 0 (Fp_C7_G)


title: Gill, Control, Day 0 (Fp_C2_G)


title: Gill, Control, Day 0 (Fp_C17_G)


title: harmine and berberine chloride biol rep 2

city: London

first: Claudia

last: Manzoni

address: 29-39 Brunswick Square

country: United Kingdom

institute: UCL

data_processing:
Sequence reads were trimmed for adapter and low quality sequence with fastp v0.23.3
Trimmed sequences were mapped to the UCSC hg38 build of the human genome by RNASTAR v2.7.10b
PCR duplicates were marked using mapping co-ordinate combined with the sequenced unique molecular identifier using JE-suite 2.0.RC
Reads were assigned to transcripts using featureCounts v2.0.3 and corresponding GTF file from iGenomes
Assembly: UCSC hg38
Supplementary files format and content: tab delimited tabular file includes raw counts for each sample
Supplementary files format and content: tab delimited tabular file includes TPM for each sample

molecule: polyA RNA

source_name: cell line

growth_protocol: SK-MEL-28 cells were plated in MEM with GlutaMaxTM + 10% fetal bovin serum + 1% non-essential amino acid + 1mM sodium pyruvate and allowed to adhere overnight (37°C, 5% CO2).

extract_protocol:
Omega Mag-Bind® Total RNA
Kapa mRNA Hyper Prep (Roche)

treatment_protocol: Cells were exposed to harmine (43μM), berberine chloride (25 μM), the combination of harmine + berberine chloride (12.5μM + 6.25μM), or the vehicle (DMSO = control) for 24hours before harvesting

value: cell line

tag: cell line

tag: cell type

value: Human Melanoma Cell Line

value: WT

value: harmine and berberine chloride

value: 24 hrs


title: harmine biol rep 3

value: harmine


title: harmine biol rep 2


title: berberine chloride biol rep 2

value: berberine chloride


title: berberine chloride biol rep 1


title: harmine and berberine chloride biol rep 3


title: Gill, Control, Day 0 (Fp_C15_G)

value: Gill

value: Uninfected


title: MG1655, 6h, R1

city: Münster

first: Kari

middle: Lavinia

last: vom Werth

address: Robert-Koch-Str. 41

department: Institute of Hygiene

country: Germany

institute: University Hospital Münster

description:
MG1655_6h_R1
Raw_read_counts_All.txt
Deseq2_MG1655_6h_vs_Ctrl_6h.txt

data_processing:
The raw sequence data were demultiplexed to fastq data using Illumina's software bcl convert (version3.8.4).
Quality control was performed with FastQC (version0.11.9).
Adapter trimming and read filtering was performed with Trimmomatic (version0.38).
Reads were aligned to the reference genome (GRCh38.108) using HISAT2 (version2.2.1).
Aligned reads were sorted with the software SAMtools (version1.9).
Counting was performed with htseq-count (version 0.11.2).
Analysis of differential gene expression was performed with the R package DESeq2 (version1.38.1).
Assembly: hg38
Supplementary files format and content: Tab-delimited text file containing raw read counts for all samples
Supplementary files format and content: Tab-delimited text files containing DESeq2 output (gene expression changes of 1h treatment vs 1h control and 6h treatment vs 6h control)

source_name: Jurkat cells

growth_protocol: Jurkat cells were maintained in RPMI supplemented with 10% fetal bovine serum (FBS) and 2 mM Ultraglutamine in humidified atmosphere with 5% CO2 at 37°C.

extract_protocol:
Total RNA was extracted using the Monarch Total RNA Miniprep Kit (NEB) with in-tube DNase treatment after elution of the RNA. The Monarch RNA Cleanup Kit (NEB) was used for subsequent purification of the RNA. Quality and quantity of the samples were evaluated on the Agilent Bioanalyzer using the Agilent RNA 6000 Nano Kit.
The NEBNext Ultra II Directional RNA Library Prep Kit (NEB) was used for library preparation following the manufacturer's instructions.

treatment_protocol: Jurkat cells were exposed to sterile bacterial culture supernatants diluted in Jurkat cell culture medium. Concentrations were adjusted to 1% (v/v) and 20% (v/v) for S.aureus and E.coli derived supernatants, respectively. The cells were incubated at 37 °C and 5% CO2 for 1h and 6h.

value: Jurkat cells

value: T lymphoblast


title: Gill, Control, Day 0 (Fp_C1_G)

value: Time point Control


title: MG1655, 1h, R3

description:
MG1655_1h_R3
Raw_read_counts_All.txt
Deseq2_MG1655_1h_vs_Ctrl_1h.txt


title: control biol rep 3

value: DMSO


title: MG1655, 1h, R1

description:
MG1655_1h_R1
Raw_read_counts_All.txt
Deseq2_MG1655_1h_vs_Ctrl_1h.txt


title: berberine chloride biol rep 3


title: control biol rep 2


title: harmine and berberine chloride biol rep 1


title: harmine biol rep 1


title: IHE3034, 6h, R1

description:
IHE3034_6h_R1
Raw_read_counts_All.txt
Deseq2_IHE3034_6h_vs_Ctrl_6h.txt


title: control biol rep 1


title: MG1655, 6h, R3

description:
MG1655_6h_R3
Raw_read_counts_All.txt
Deseq2_MG1655_6h_vs_Ctrl_6h.txt


title: MG1655, 6h, R2

description:
MG1655_6h_R2
Raw_read_counts_All.txt
Deseq2_MG1655_6h_vs_Ctrl_6h.txt


title: IHE3034, 1h, R3

description:
IHE3034_1h_R3
Raw_read_counts_All.txt
Deseq2_IHE3034_1h_vs_Ctrl_1h.txt


title: 536, 6h, R2

description:
536_6h_R2
Raw_read_counts_All.txt
Deseq2_536_6h_vs_Ctrl_6h.txt


title: IHE3034, 6h, R3

description:
IHE3034_6h_R3
Raw_read_counts_All.txt
Deseq2_IHE3034_6h_vs_Ctrl_6h.txt


title: IHE3034, 6h, R2

description:
IHE3034_6h_R2
Raw_read_counts_All.txt
Deseq2_IHE3034_6h_vs_Ctrl_6h.txt


title: MG1655, 1h, R2

description:
MG1655_1h_R2
Raw_read_counts_All.txt
Deseq2_MG1655_1h_vs_Ctrl_1h.txt


title: IHE3034, 1h, R2

description:
IHE3034_1h_R2
Raw_read_counts_All.txt
Deseq2_IHE3034_1h_vs_Ctrl_1h.txt


title: 536, 1h, R2

description:
536_1h_R2
Raw_read_counts_All.txt
Deseq2_536_1h_vs_Ctrl_1h.txt


title: ST398, 6h, R3

description:
ST398_6h_R3
Raw_read_counts_All.txt
Deseq2_ST398_6h_vs_Ctrl_6h.txt


title: IHE3034, 1h, R1

description:
IHE3034_1h_R1
Raw_read_counts_All.txt
Deseq2_IHE3034_1h_vs_Ctrl_1h.txt


title: USA300, 6h, R3

description:
USA300_6h_R3
Raw_read_counts_All.txt
Deseq2_USA300_6h_vs_Ctrl_6h.txt


title: 536, 1h, R3

description:
536_1h_R3
Raw_read_counts_All.txt
Deseq2_536_1h_vs_Ctrl_1h.txt


title: 536, 1h, R1

description:
536_1h_R1
Raw_read_counts_All.txt
Deseq2_536_1h_vs_Ctrl_1h.txt


title: ST398, 6h, R2

description:
ST398_6h_R2
Raw_read_counts_All.txt
Deseq2_ST398_6h_vs_Ctrl_6h.txt


title: ST398, 6h, R1

description:
ST398_6h_R1
Raw_read_counts_All.txt
Deseq2_ST398_6h_vs_Ctrl_6h.txt


title: ST398, 1h, R1

description:
ST398_1h_R1
Raw_read_counts_All.txt
Deseq2_ST398_1h_vs_Ctrl_1h.txt


title: ST398, 1h, R2

description:
ST398_1h_R2
Raw_read_counts_All.txt
Deseq2_ST398_1h_vs_Ctrl_1h.txt


title: ST398, 1h, R3

description:
ST398_1h_R3
Raw_read_counts_All.txt
Deseq2_ST398_1h_vs_Ctrl_1h.txt


title: USA300, 6h, R2

description:
USA300_6h_R2
Raw_read_counts_All.txt
Deseq2_USA300_6h_vs_Ctrl_6h.txt


title: 536, 6h, R3

description:
536_6h_R3
Raw_read_counts_All.txt
Deseq2_536_6h_vs_Ctrl_6h.txt


title: USA300, 6h, R1

description:
USA300_6h_R1
Raw_read_counts_All.txt
Deseq2_USA300_6h_vs_Ctrl_6h.txt


title: USA300, 1h, R3

description:
USA300_1h_R3
Raw_read_counts_All.txt
Deseq2_USA300_1h_vs_Ctrl_1h.txt


title: Ctrl, 1h, R3

description:
Ctrl_1h_R3
Raw_read_counts_All.txt


title: Ctrl, 1h, R1

description:
Ctrl_1h_R1
Raw_read_counts_All.txt


title: Ctrl, 1h, R2

description:
Ctrl_1h_R2
Raw_read_counts_All.txt


title: Ctrl, 0h, R2

description:
Ctrl_0h_R2
Raw_read_counts_All.txt


title: Ctrl, 6h, R3

description:
Ctrl_6h_R3
Raw_read_counts_All.txt


title: USA300, 1h, R1

description:
USA300_1h_R1
Raw_read_counts_All.txt
Deseq2_USA300_1h_vs_Ctrl_1h.txt


title: SupT1, Latent, ATAC, rep2

city: Ithaca

first: Gabrielle

last: Lê-Bury

address: 930 campus road

country: USA

institute: Cornell University

library_source: genomic

data_processing:
Raw ATAC-seq reads were first processed by FastQC for quality control and then Illumina sequencing adapters were trimmed from paired-end reads using Cutadapt
The trimmed reads were aligned to human reference genome GRCh38 using BWA-MEM and the resulting alignment output were analyzed by SAMStat for quality control and then sorted by SAMtools
Post-alignment filtering was performed by SAMtools, ‘MarkDuplicates’ program of Picard tools as well as ENCODE ATAC-seq script assign_multimappers.py to remove PCR duplicates, secondary alignments and unmapped reads. Quality control of fragment size distribution was carried out by ATACseqQC
Assembly: GRCh38
Supplementary files format and content: tab-delimited text files include merged IDR peak counts

molecule: genomic DNA

growth_protocol: SupT1 cells were cultured in RPMI media supplemented with 10% fetal bovine serum (FBS) and 1% each of penicillin, streptomycin and glutamine

extract_protocol:
100,000 cells were used to for transposition reaction (Illumina Tagment DNA TDE1 Enzyme and Buffer Kit). Tagmented DNA was purified with MinElute purification kit (Qiagen)
Tagmented DNA used for subsequent library preparation by PCR amplification using unique dual-indexing (Illumina Nextera i5 common adapter and i7 index adapter) primers. ATAC-seq libraries were purified using AMPure SPRI beads (Beckman Coulter)

treatment_protocol: SupT1 cells were infected with HIVGKO virus at an multiplicity of infection of 0.1 by spinoculation (3000rpm, 30min, 30C). Virus-infected or uninfected cells were cultured for 4 days in a 37C incubator with 6% CO2. Uninfected or HIV-1 active-infected (mKO2+GFP+) and latent- infected (mKO2+GFP-) SupT1 cells were flow sorted using a Biorad S3 cell sorter

value: HIV GKO infection

value: 4 days post infection


title: SupT1, Active, ATAC, rep2


title: SupT1, Active, ATAC, rep3


title: Ctrl, 6h, R1

description:
Ctrl_6h_R1
Raw_read_counts_All.txt


title: Ctrl, 0h, R1

description:
Ctrl_0h_R1
Raw_read_counts_All.txt


title: SupT1, Latent, ATAC, rep1


title: SupT1, Latent, ATAC, rep3


title: SupT1, Active, ATAC, rep1


title: USA300, 1h, R2

description:
USA300_1h_R2
Raw_read_counts_All.txt
Deseq2_USA300_1h_vs_Ctrl_1h.txt


title: Ctrl, 0h, R3

description:
Ctrl_0h_R3
Raw_read_counts_All.txt


title: SupT1, Uninfected, ATAC, rep3


title: 536, 6h, R1

description:
536_6h_R1
Raw_read_counts_All.txt
Deseq2_536_6h_vs_Ctrl_6h.txt


title: 220718L48_exp220610_MS63_SCGE-16nM-BE_gDNA-seq-rep2

city: Stanford

first: Georgi

middle: Kolev

last: Marinov

state: California

address: 279 Campus Drive West, Beckman Center, B-257A/259

department: Genetics

institute: STANFORD UNIVERSITY

description: gDNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 16nM beta-estradiol.

data_processing:
read alignment, ChIP-seq and gDNA data Bowtie (version 1.0.1)
read alignment, RNA-seq data STAR (version 2.5.3a
bigWig file generation custom code
Assembly: sacCer3
Supplementary files format and content: bigWig
Library strategy: Tn5 gDNA-seq

organism:
Saccharomyces cerevisiae
Nakaseomyces glabratus

growth_protocol:
S. pombe: Standard yeast cell culture practices were followed. Cells were cultured at 30C  in Edinburgh Minimal Media (EMM).
C. Glabrata: Standard yeast cell culture practices were followed. Cells were cultured at 30C  in synthetic complete media with either 2% glucose (SCD) or 2% glycerol + 1% ethanol (SCGE) as a carbon source.
S. cerevisiae: Standard yeast cell culture practices were followed. Cells were cultured at 30C  in synthetic complete media with either 2% glucose (SCD) or 2% glycerol + 1% ethanol (SCGE) as a carbon source.

extract_protocol:
S. cerevisiae and C. glabrata cells were separately pelleted and snap-frozen in liquid nitrogen. Pellets were thawed in ice-cold PBS and mixed. 20 µl of cells in PBS were added to 110 µl YD Digestion Buffer (YeaStar Genomic DNA Kit; ZymoResarch) and gDNA was extracted according to Protocol 1 for the YeaStar Genomic DNA Kit (Zymo Research).
4ng gDNA was used for prepared for sequencing by tagmentation with custom-made Tn5 (30 minutes at 37 °C) and then cleaned up (MinElute PCR Purification Kit, Quiagen). Tagmented DNA was amplified (7 cycles) with NEBNext High-Fidelity 2X PCR Master Mix (M0541). Libraries were then cleaned up using AMpure XP beads (Beckman Coulter), pooled, and sequenced by paired-end (2x150 bp) Illumina sequencing.
Tn5 gDNA-seq

tag: sample

value: yeast

tag: chip-antibody


title: SupT1, Uninfected, ATAC, rep2


title: 220718L47_exp220610_MS63_SCGE-3.5nM-BE_gDNA-seq-rep2

description: gDNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 3.5nM beta-estradiol.


title: SupT1, Uninfected, ATAC, rep1


title: 220718L45_exp220610_MS81_SCGE_gDNA-seq-rep2

description: gDNA from a mixture S. cerevisiae (MS81) and C. glabrata (MS99).


title: Ctrl, 6h, R2

description:
Ctrl_6h_R2
Raw_read_counts_All.txt


title: 220718L39_exp220607_MS81_SCGE_gDNA-seq-rep2

value: gDNA from a mixture S. cerevisiae (MS81) and C. glabrata (MS99).


title: 220718L46_exp220610_MS63_SCGE-00nM-BE_gDNA-seq-rep2

description: gDNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99).


title: 220718L41_exp220607_MS63_SCGE-3.5nM-BE_gDNA-seq-rep2

value: gDNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 3.5nM beta-estradiol.


title: 220718L43_exp220610_MS01_SCGE_gDNA-seq-rep2

description: gDNA from a mixture S. cerevisiae (MS01) and C. glabrata (MS99).


title: 220718L37_exp220607_MS01_SCGE_gDNA-seq-rep2

value: gDNA from a mixture S. cerevisiae (MS01) and C. glabrata (MS99).


title: 220718L36_exp220601_MS63_SCGE-15nM-BE_gDNA-seq-rep2

description: gDNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 15nM beta-estradiol.


title: 220718L42_exp220607_MS63_SCGE-16nM-BE_gDNA-seq-rep2

value: gDNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 16nM beta-estradiol.


title: 220718L40_exp220607_MS63_SCGE-00nM-BE_gDNA-seq-rep2

value: gDNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99).


title: 220718L35_exp220601_MS63_SCGE-04nM-BE_gDNA-seq-rep2

description: gDNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 4nM beta-estradiol.


title: 220718L38_exp220607_MS80_SCGE_gDNA-seq-rep2

description: gDNA from a mixture S. cerevisiae (MS80) and C. glabrata (MS99).


title: 220718L34_exp220601_MS63_SCGE-00nM-BE_gDNA-seq-rep2


title: 220718L32_exp220601_MS80_SCGE_gDNA-seq-rep2

value: gDNA from a mixture S. cerevisiae (MS80) and C. glabrata (MS99).


title: 220718L30_exp220519_MS63_SCGE-15nM-BE_gDNA-seq-rep2

value: gDNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 15nM beta-estradiol.


title: 220718L44_exp220610_MS80_SCGE_gDNA-seq-rep2


title: 220718L27_exp220519_MS81_SCGE_gDNA-seq-rep2


title: 220718L31_exp220601_MS01_SCGE_gDNA-seq-rep2


title: 220718L29_exp220519_MS63_SCGE-04nM-BE_gDNA-seq-rep2

value: gDNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 4nM beta-estradiol.


title: 220718L28_exp220519_MS63_SCGE-00nM-BE_gDNA-seq-rep2


title: 220718L26_exp220519_MS80_SCGE_gDNA-seq-rep2


title: 220718L23_exp220610_MS63_SCGE-3.5nM-BE_gDNA-seq-rep1


title: 220718L22_exp220610_MS63_SCGE-00nM-BE_gDNA-seq-rep1


title: 220718L20_exp220610_MS80_SCGE_gDNA-seq-rep1


title: 220718L21_exp220610_MS81_SCGE_gDNA-seq-rep1


title: 220718L24_exp220610_MS63_SCGE-16nM-BE_gDNA-seq-rep1


title: 220718L25_exp220519_MS01_SCGE_gDNA-seq-rep2


title: 220718L18_exp220607_MS63_SCGE-16nM-BE_gDNA-seq-rep1


title: 220718L17_exp220607_MS63_SCGE-3.5nM-BE_gDNA-seq-rep1


title: 220718L19_exp220610_MS01_SCGE_gDNA-seq-rep1


title: 220718L16_exp220607_MS63_SCGE-00nM-BE_gDNA-seq-rep1


title: 220718L33_exp220601_MS81_SCGE_gDNA-seq-rep2


title: 220718L14_exp220607_MS80_SCGE_gDNA-seq-rep1


title: 220718L12_exp220601_MS63_SCGE-15nM-BE_gDNA-seq-rep1


title: 220718L13_exp220607_MS01_SCGE_gDNA-seq-rep1


title: 220718L11_exp220601_MS63_SCGE-04nM-BE_gDNA-seq-rep1


title: 220718L15_exp220607_MS81_SCGE_gDNA-seq-rep1


title: 220718L10_exp220601_MS63_SCGE-00nM-BE_gDNA-seq-rep1


title: 220718L09_exp220601_MS81_SCGE_gDNA-seq-rep1


title: 220718L06_exp220519_MS63_SCGE-15nM-BE_gDNA-seq-rep1


title: 220718L08_exp220601_MS80_SCGE_gDNA-seq-rep1


title: 220718L07_exp220601_MS01_SCGE_gDNA-seq-rep1


title: 220718L05_exp220519_MS63_SCGE-04nM-BE_gDNA-seq-rep1


title: 220718L04_exp220519_MS63_SCGE-00nM-BE_gDNA-seq-rep1


title: 220718L03_exp220519_MS81_SCGE_gDNA-seq-rep1


title: 220819L24_exp220607_MS63_SCGE-16nM-BE_polyA-RNA-seq

description: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 16nM beta-estradiol.

data_processing:
read alignment, ChIP-seq and gDNA data Bowtie (version 1.0.1)
read alignment, RNA-seq data STAR (version 2.5.3a
bigWig file generation custom code
Assembly: sacCer3
Supplementary files format and content: bigWig

extract_protocol:
S. cerevisiae and C. glabrata cells were separately pelleted and snap-frozen in liquid nitrogen. Pellets were thawed in ice-cold PBS and mixed. 30 µl of cells in PBS were added to 300 µl TRI Reagent (Zymo Research) and lysed by bead beating using a Fastprep 24. Cell debris was pelleted (14k rpm, 1 minute) and the supernatant recovered. RNA was then extracted using the direct-zol RNA microprep kit (Zymo Research). mRNA was enriched using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490).
Indexed sequencing libraries were generated using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, #E7775), pooled, and then sequenced by paired-end (2x150bp) Illumina sequencing.
spike-in normalised polyA RNA-seq


title: 220819L23_exp220607_MS63_SCGE-3.5nM-BE_polyA-RNA-seq

description: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 3.5nM beta-estradiol.


title: 220819L22_exp220607_MS63_SCGE-00nM-BE_polyA-RNA-seq

description: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99).


title: 220718L02_exp220519_MS80_SCGE_gDNA-seq-rep1


title: 220819L20_exp220607_MS80_SCGE_polyA-RNA-seq

description: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS80) and C. glabrata (MS99).


title: 220819L21_exp220607_MS81_SCGE_polyA-RNA-seq

description: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS81) and C. glabrata (MS99).


title: 220819L16_exp220610_MS63_SCGE-00nM-BE_polyA-RNA-seq

value: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99).


title: 220819L19_exp220607_MS01_SCGE_polyA-RNA-seq

description: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS01) and C. glabrata (MS99).


title: 220718L01_exp220519_MS01_SCGE_gDNA-seq-rep1


title: 220819L15_exp220610_MS81_SCGE_polyA-RNA-seq

value: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS81) and C. glabrata (MS99).


title: 220819L14_exp220610_MS80_SCGE_polyA-RNA-seq

value: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS80) and C. glabrata (MS99).


title: 220819L18_exp220610_MS63_SCGE-16nM-BE_polyA-RNA-seq

value: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 16nM beta-estradiol.


title: 220819L13_exp220610_MS01_SCGE_polyA-RNA-seq

value: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS01) and C. glabrata (MS99).


title: 220819L17_exp220610_MS63_SCGE-3.5nM-BE_polyA-RNA-seq

value: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 3.5nM beta-estradiol.


title: 220819L11_exp220601_MS63_SCGE-04nM-BE_polyA-RNA-seq

description: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 4nM beta-estradiol.


title: 220819L10_exp220601_MS63_SCGE-00nM-BE_polyA-RNA-seq


title: 220819L08_exp220601_MS80_SCGE_polyA-RNA-seq


title: 220819L07_exp220601_MS01_SCGE_polyA-RNA-seq


title: 220819L06_exp220519_MS63_SCGE-15nM-BE_polyA-RNA-seq

description: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 15nM beta-estradiol.


title: 220819L09_exp220601_MS81_SCGE_polyA-RNA-seq


title: 220819L04_exp220519_MS63_SCGE-00nM-BE_polyA-RNA-seq


title: 220819L05_exp220519_MS63_SCGE-04nM-BE_polyA-RNA-seq

value: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 4nM beta-estradiol.


title: 220819L03_exp220519_MS81_SCGE_polyA-RNA-seq


title: 220819L01_exp220519_MS01_SCGE_polyA-RNA-seq


title: 220323L26_exp211101_MS67_SCGE_T05_gDNA-seq-rep1

description: gDNA from a mixture S. cerevisiae (MS67) and C. glabrata (MS99). MS67 was arrested in G1 and collected at timepoint 5.


title: 220323L27_exp211101_MS67_SCGE_T06_gDNA-seq-rep1

description: gDNA from a mixture S. cerevisiae (MS67) and C. glabrata (MS99). MS67 was arrested in G1 and collected at timepoint 6.


title: 220819L02_exp220519_MS80_SCGE_polyA-RNA-seq


title: 220323L23_exp211101_MS67_SCGE_T02_gDNA-seq-rep1

description: gDNA from a mixture S. cerevisiae (MS67) and C. glabrata (MS99). MS67 was arrested in G1 and collected at timepoint 2.


title: 220323L22_exp211101_MS67_SCGE_T01_gDNA-seq-rep1

description: gDNA from a mixture S. cerevisiae (MS67) and C. glabrata (MS99). MS67 was arrested in G1 and collected at timepoint 1.


title: 220323L24_exp211101_MS67_SCGE_T03_gDNA-seq-rep1

description: gDNA from a mixture S. cerevisiae (MS67) and C. glabrata (MS99). MS67 was arrested in G1 and collected at timepoint 3.


title: 220323L20_exp211028_MS64_SCGE_T06_gDNA-seq-rep1

description: gDNA from a mixture S. cerevisiae (MS64) and C. glabrata (MS99). MS64 was arrested in G1 and collected at timepoint 6.


title: 220323L25_exp211101_MS67_SCGE_T04_gDNA-seq-rep1

description: gDNA from a mixture S. cerevisiae (MS67) and C. glabrata (MS99). MS67 was arrested in G1 and collected at timepoint 4.


title: 220323L19_exp211028_MS64_SCGE_T05_gDNA-seq-rep1

description: gDNA from a mixture S. cerevisiae (MS64) and C. glabrata (MS99). MS64 was arrested in G1 and collected at timepoint 5.


title: 220323L16_exp211028_MS64_SCGE_T02_gDNA-seq-rep1

description: gDNA from a mixture S. cerevisiae (MS64) and C. glabrata (MS99). MS64 was arrested in G1 and collected at timepoint 2.


title: 220323L18_exp211028_MS64_SCGE_T04_gDNA-seq-rep1

description: gDNA from a mixture S. cerevisiae (MS64) and C. glabrata (MS99). MS64 was arrested in G1 and collected at timepoint 4.


title: 220819L12_exp220601_MS63_SCGE-15nM-BE_polyA-RNA-seq

value: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 15nM beta-estradiol.


title: 220323L17_exp211028_MS64_SCGE_T03_gDNA-seq-rep1

description: gDNA from a mixture S. cerevisiae (MS64) and C. glabrata (MS99). MS64 was arrested in G1 and collected at timepoint 3.

value: yeast


title: 220323L15_exp211028_MS64_SCGE_T01_gDNA-seq-rep1

description: gDNA from a mixture S. cerevisiae (MS64) and C. glabrata (MS99). MS64 was arrested in G1 and collected at timepoint 1.


title: 220323L21_exp211028_MS64_SCGE_T07_gDNA-seq-rep1

description: gDNA from a mixture S. cerevisiae (MS64) and C. glabrata (MS99). MS64 was arrested in G1 and collected at timepoint 7.


title: 220323L14_exp211021_MS67_SCGE_T07_gDNA-seq-rep1

description: gDNA from a mixture S. cerevisiae (MS67) and C. glabrata (MS99). MS67 was arrested in G1 and collected at timepoint 7.


title: 220323L12_exp211021_MS67_SCGE_T05_gDNA-seq-rep1

value: gDNA from a mixture S. cerevisiae (MS67) and C. glabrata (MS99). MS67 was arrested in G1 and collected at timepoint 5.


title: 220323L13_exp211021_MS67_SCGE_T06_gDNA-seq-rep1

value: gDNA from a mixture S. cerevisiae (MS67) and C. glabrata (MS99). MS67 was arrested in G1 and collected at timepoint 6.


title: 220323L09_exp211021_MS67_SCGE_T02_gDNA-seq-rep1

value: gDNA from a mixture S. cerevisiae (MS67) and C. glabrata (MS99). MS67 was arrested in G1 and collected at timepoint 2.


title: 220323L08_exp211021_MS67_SCGE_T01_gDNA-seq-rep1

value: gDNA from a mixture S. cerevisiae (MS67) and C. glabrata (MS99). MS67 was arrested in G1 and collected at timepoint 1.


title: 220323L10_exp211021_MS67_SCGE_T03_gDNA-seq-rep1

value: gDNA from a mixture S. cerevisiae (MS67) and C. glabrata (MS99). MS67 was arrested in G1 and collected at timepoint 3.


title: 220323L07_exp211025_MS64_SCGE_T07_gDNA-seq-rep1

value: gDNA from a mixture S. cerevisiae (MS64) and C. glabrata (MS99). MS64 was arrested in G1 and collected at timepoint 7.


title: 220323L06_exp211025_MS64_SCGE_T06_gDNA-seq-rep1

value: gDNA from a mixture S. cerevisiae (MS64) and C. glabrata (MS99). MS64 was arrested in G1 and collected at timepoint 6.


title: 220323L05_exp211025_MS64_SCGE_T05_gDNA-seq-rep1

value: gDNA from a mixture S. cerevisiae (MS64) and C. glabrata (MS99). MS64 was arrested in G1 and collected at timepoint 5.


title: 220323L28_exp211101_MS67_SCGE_T07_gDNA-seq-rep1

value: gDNA from a mixture S. cerevisiae (MS67) and C. glabrata (MS99). MS67 was arrested in G1 and collected at timepoint 7.


title: 220323L02_exp211025_MS64_SCGE_T02_gDNA-seq-rep1

value: gDNA from a mixture S. cerevisiae (MS64) and C. glabrata (MS99). MS64 was arrested in G1 and collected at timepoint 2.


title: 220328R28_exp211101_MS67_SCGE-T07_polyA-RNA-seq

description: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS67) and C. glabrata (MS99). MS67 was arrested in G1 and collected at timepoint 7.


title: 220328R27_exp211101_MS67_SCGE-T06_polyA-RNA-seq

description: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS67) and C. glabrata (MS99). MS67 was arrested in G1 and collected at timepoint 6.


title: 220323L03_exp211025_MS64_SCGE_T03_gDNA-seq-rep1

value: gDNA from a mixture S. cerevisiae (MS64) and C. glabrata (MS99). MS64 was arrested in G1 and collected at timepoint 3.


title: 220328R25_exp211101_MS67_SCGE-T04_polyA-RNA-seq

description: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS67) and C. glabrata (MS99). MS67 was arrested in G1 and collected at timepoint 4.


title: 220328R24_exp211101_MS67_SCGE-T03_polyA-RNA-seq

description: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS67) and C. glabrata (MS99). MS67 was arrested in G1 and collected at timepoint 3.


title: 220328R20_exp211028_MS64_SCGE-T06_polyA-RNA-seq

description: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS64) and C. glabrata (MS99). MS64 was arrested in G1 and collected at timepoint 6.


title: 220328R18_exp211028_MS64_SCGE-T04_polyA-RNA-seq

description: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS64) and C. glabrata (MS99). MS64 was arrested in G1 and collected at timepoint 4.


title: 220328R22_exp211101_MS67_SCGE-T01_polyA-RNA-seq

description: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS67) and C. glabrata (MS99). MS67 was arrested in G1 and collected at timepoint 1.


title: 220323L11_exp211021_MS67_SCGE_T04_gDNA-seq-rep1

value: gDNA from a mixture S. cerevisiae (MS67) and C. glabrata (MS99). MS67 was arrested in G1 and collected at timepoint 4.


title: 220328R19_exp211028_MS64_SCGE-T05_polyA-RNA-seq

description: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS64) and C. glabrata (MS99). MS64 was arrested in G1 and collected at timepoint 5.


title: 220328R14_exp211021_MS67_SCGE-T07_polyA-RNA-seq

value: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS67) and C. glabrata (MS99). MS67 was arrested in G1 and collected at timepoint 7.


title: 220328R23_exp211101_MS67_SCGE-T02_polyA-RNA-seq

description: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS67) and C. glabrata (MS99). MS67 was arrested in G1 and collected at timepoint 2.


title: 220328R21_exp211028_MS64_SCGE-T07_polyA-RNA-seq

description: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS64) and C. glabrata (MS99). MS64 was arrested in G1 and collected at timepoint 7.


title: 220328R15_exp211028_MS64_SCGE-T01_polyA-RNA-seq

description: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS64) and C. glabrata (MS99). MS64 was arrested in G1 and collected at timepoint 1.


title: 220328R16_exp211028_MS64_SCGE-T02_polyA-RNA-seq

description: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS64) and C. glabrata (MS99). MS64 was arrested in G1 and collected at timepoint 2.


title: 220323L04_exp211025_MS64_SCGE_T04_gDNA-seq-rep1

description: gDNA from a mixture S. cerevisiae (MS64) and C. glabrata (MS99). MS64 was arrested in G1 and collected at timepoint 4.


title: 220328R11_exp211021_MS67_SCGE-T04_polyA-RNA-seq

value: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS67) and C. glabrata (MS99). MS67 was arrested in G1 and collected at timepoint 4.


title: 220328R12_exp211021_MS67_SCGE-T05_polyA-RNA-seq

description: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS67) and C. glabrata (MS99). MS67 was arrested in G1 and collected at timepoint 5.


title: 220328R17_exp211028_MS64_SCGE-T03_polyA-RNA-seq

description: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS64) and C. glabrata (MS99). MS64 was arrested in G1 and collected at timepoint 3.


title: 220328R10_exp211021_MS67_SCGE-T03_polyA-RNA-seq

value: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS67) and C. glabrata (MS99). MS67 was arrested in G1 and collected at timepoint 3.


title: 220328R09_exp211021_MS67_SCGE-T02_polyA-RNA-seq

value: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS67) and C. glabrata (MS99). MS67 was arrested in G1 and collected at timepoint 2.


title: 220328R13_exp211021_MS67_SCGE-T06_polyA-RNA-seq

value: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS67) and C. glabrata (MS99). MS67 was arrested in G1 and collected at timepoint 6.

value: yeast


title: 220328R26_exp211101_MS67_SCGE-T05_polyA-RNA-seq

value: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS67) and C. glabrata (MS99). MS67 was arrested in G1 and collected at timepoint 5.


title: 220328R08_exp211021_MS67_SCGE-T01_polyA-RNA-seq

value: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS67) and C. glabrata (MS99). MS67 was arrested in G1 and collected at timepoint 1.


title: 220328R04_exp211025_MS64_SCGE-T04_polyA-RNA-seq

value: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS64) and C. glabrata (MS99). MS64 was arrested in G1 and collected at timepoint 4.


title: 220328R06_exp211025_MS64_SCGE-T06_polyA-RNA-seq

value: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS64) and C. glabrata (MS99). MS64 was arrested in G1 and collected at timepoint 6.


title: 220323L01_exp211025_MS64_SCGE_T01_gDNA-seq-rep1

description: gDNA from a mixture S. cerevisiae (MS64) and C. glabrata (MS99). MS64 was arrested in G1 and collected at timepoint 1.


title: 220328R03_exp211025_MS64_SCGE-T03_polyA-RNA-seq

value: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS64) and C. glabrata (MS99). MS64 was arrested in G1 and collected at timepoint 3.


title: 220328R01_exp211025_MS64_SCGE-T01_polyA-RNA-seq

value: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS64) and C. glabrata (MS99). MS64 was arrested in G1 and collected at timepoint 1.


title: 220328R02_exp211025_MS64_SCGE-T02_polyA-RNA-seq

value: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS64) and C. glabrata (MS99). MS64 was arrested in G1 and collected at timepoint 2.


title: 210325L26_exp210314_MS698_SCD-50-ATC_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS698) and C. glabrata (MS99). MS698 was treated with anhydrotetracycline before collection.

extract_protocol:
S. cerevisiae cultures were combined with spike-in cultures (C. glabrata or S. pombe) and immediately fixed  with formaldehyde at a final concentration of 1% for 15 minutes. Cells were then quenched with 0.125 M glycine (5 minutes), washed twice in cold PBS, pelleted, snap-frozen, and stored at −80 °C. Pellets were thawed and lysed in 300 µl FA lysis buffer (50 mM HEPES–KOH pH 8.0, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM PMSF) with ∼1 ml ceramic beads on a Fastprep-24 (MP Biomedicals). The entire lysate was then collected and adjusted to 1 ml before sonication with a 1/8” microtip on a Q500 sonicator (Qsonica) for 8-16 minutes (cycles of 10 seconds on and 20 seconds off). The sample tube was held suspended in a −20 °C 80% ethanol bath to prevent sample heating during sonication. Cell debris was then pelleted, and the supernatant was retained for ChIP or input. For each ChIP reaction, 20 µl Protein G Dynabeads (Invitrogen) were blocked (PBS + 0.5% BSA, incubate 40 minutes at room temperature), pre-bound with 5 µl of antibody in PBS (incubate 40 minutes at room temperature), and washed 2x with PBS before being incubated with supernatant (4°C overnight). Dynabeads were then washed (5 minutes per wash) 2x in FA lysis buffer and 3x in high-salt FA lysis buffer (50 mM Hepes-KOH pH 8.0, 500 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM PMSF) and DNA was then eluted in ChIP elution buffer (50 mM TrisHCl pH 7.5, 10 mM EDTA, 1% SDS) at 65 °C for 20 minutes. 15 µl of input was mixed directly with 115 µl of ChIP elution buffer for input samples. Crosslinks were then reversed (65 °C, 5hr) before treatment with RNAse A (37 °C, 1 hour) and then Proteinase K (65 °C, 2 hours). DNA was then purified using the ChIP DNA Clean & Concentrator kit (Zymo Research).
Indexed sequencing libraries were generated using the NEBNext Ultra II DNA Library Prep kit (NEB Cat # E7645), pooled, and then sequenced by paired-end (2x150bp) Illumina sequencing.
spike-in normalised ChIP-seq

value: anti-Rpb1 (8wG16; Millipore # 05-952-I)


title: 210325L25_exp210314_MS698_SCD-EtOH_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS698) and C. glabrata (MS99). MS698 was treated with ethanol (mock) before collection.


title: 210325L20_exp210314_MS698_SCD-50-ATC_anti-Rpb1

value: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS698) and C. glabrata (MS99). MS698 was treated with anhydrotetracycline before collection.


title: 210325L21_exp210314_MS214_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS214) and C. glabrata (MS99). MS214 was treated with rapamycin before collection.


title: 210325L09_exp210314_MS214_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS214) and C. glabrata (MS99). MS214 was treated with rapamycin before collection.


title: 210325L19_exp210314_MS698_SCD-EtOH_anti-Rpb1

value: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS698) and C. glabrata (MS99). MS698 was treated with ethanol (mock) before collection.


title: 210325L03_exp210314_MS214_SCD-RAPA_input

value: Input ChIP DNA from a mixture S. cerevisiae (MS214) and C. glabrata (MS99). MS214 was treated with rapamycin before collection.


title: 210325L07_exp210314_MS698_SCD-EtOH_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS698) and C. glabrata (MS99). MS698 was treated with ethanol (mock) before collection.


title: 210325L08_exp210314_MS698_SCD-50-ATC_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS698) and C. glabrata (MS99). MS698 was treated with anhydrotetracycline before collection.


title: 220328R07_exp211025_MS64_SCGE-T07_polyA-RNA-seq

value: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS64) and C. glabrata (MS99). MS64 was arrested in G1 and collected at timepoint 7.


title: 210128L14_exp210122_MS205_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS205) and C. glabrata (MS99). MS205 was treated with rapamycin before collection.


title: 210325L01_exp210314_MS698_SCD-EtOH_input

value: Input ChIP DNA from a mixture S. cerevisiae (MS698) and C. glabrata (MS99). MS698 was treated with ethanol (mock) before collection.


title: 210128L09_exp210122_MS656_SCD-EtOH_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS656) and C. glabrata (MS99). MS656 was treated with ethanol (mock) before collection.


title: 210128L13_exp210122_MS656_SCD-50-ATC_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS656) and C. glabrata (MS99). MS656 was treated with anhydrotetracycline before collection.


title: 210128L04_exp210122_MS656_SCD-50-ATC_anti-Rpb1

value: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS656) and C. glabrata (MS99). MS656 was treated with anhydrotetracycline before collection.


title: 210128L23_exp210122_MS656_SCD-EtOH_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS656) and C. glabrata (MS99). MS656 was treated with ethanol (mock) before collection.


title: 210128L21_exp210122_MS205_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS205) and C. glabrata (MS99). MS205 was treated with rapamycin before collection.


title: 210128L02_exp210122_MS656_SCD-EtOH_anti-Rpb1

value: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS656) and C. glabrata (MS99). MS656 was treated with ethanol (mock) before collection.


title: 210128L18_exp210122_MS656_SCD-50-ATC_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS656) and C. glabrata (MS99). MS656 was treated with anhydrotetracycline before collection.


title: 210128L07_exp210122_MS205_SCD-RAPA_anti-Rpb1

value: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS205) and C. glabrata (MS99). MS205 was treated with rapamycin before collection.


title: 210128L16_exp210122_MS656_SCD-EtOH_input

value: Input ChIP DNA from a mixture S. cerevisiae (MS656) and C. glabrata (MS99). MS656 was treated with ethanol (mock) before collection.

value: yeast


title: 200917L04_exp200803_MS282_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS289) and C. glabrata (MS99). MS289 was treated with rapamycin before collection.


title: 200917L12_exp200803_MS289_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS299) and C. glabrata (MS99). MS299 was treated with rapamycin before collection.


title: 220328R05_exp211025_MS64_SCGE-T05_polyA-RNA-seq

description: cDNA of polyA-enriched RNA from a mixture S. cerevisiae (MS64) and C. glabrata (MS99). MS64 was arrested in G1 and collected at timepoint 5.


title: 200917L10_exp200803_MS294_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS282) and C. glabrata (MS99). MS282 was treated with rapamycin before collection.


title: 210325L27_exp210314_MS214_SCD-RAPA_anti-Rpb1

value: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS214) and C. glabrata (MS99). MS214 was treated with rapamycin before collection.


title: 200917L08_exp200803_MS281_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS207) and C. glabrata (MS99). MS207 was treated with rapamycin before collection.


title: 200917L07_exp200803_MS207_SCD-RAPA_anti-Rpb1

value: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS289) and C. glabrata (MS99). MS289 was treated with rapamycin before collection.


title: 210325L02_exp210314_MS698_SCD-50-ATC_input

value: Input ChIP DNA from a mixture S. cerevisiae (MS698) and C. glabrata (MS99). MS698 was treated with anhydrotetracycline before collection.


title: 200917L02_exp200802_MS208_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS208) and C. glabrata (MS99). MS208 was treated with rapamycin before collection.


title: 210128L28_exp210122_MS205_SCD-RAPA_input

value: Input ChIP DNA from a mixture S. cerevisiae (MS205) and C. glabrata (MS99). MS205 was treated with rapamycin before collection.


title: 200917L05_exp200802_MS295_SCD-RAPA_anti-Rpb1

value: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS282) and C. glabrata (MS99). MS282 was treated with rapamycin before collection.


title: 210128L27_exp210122_MS656_SCD-50-ATC_input

value: Input ChIP DNA from a mixture S. cerevisiae (MS656) and C. glabrata (MS99). MS656 was treated with anhydrotetracycline before collection.


title: 200917L06_exp200802_MS289_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS295) and C. glabrata (MS99). MS295 was treated with rapamycin before collection.

value: anti-Rpb1 (8wG16; Millipore # 05-952-I)


title: 200917L32_exp200803_MS282_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS282) and C. glabrata (MS99). MS282 was treated with rapamycin before collection.


title: 200917L01_exp200802_MS207_SCD-RAPA_anti-Rpb1

value: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS207) and C. glabrata (MS99). MS207 was treated with rapamycin before collection.


title: 200917L36_exp200803_MS281_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS281) and C. glabrata (MS99). MS281 was treated with rapamycin before collection.


title: 200917L39_exp200803_MS299_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS299) and C. glabrata (MS99). MS299 was treated with rapamycin before collection.


title: 200917L35_exp200803_MS207_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS207) and C. glabrata (MS99). MS207 was treated with rapamycin before collection.


title: 200917L33_exp200802_MS295_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS295) and C. glabrata (MS99). MS295 was treated with rapamycin before collection.


title: 200917L11_exp200803_MS299_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS294) and C. glabrata (MS99). MS294 was treated with rapamycin before collection.


title: 200917L37_exp200802_MS282_SCD-RAPA_input

value: Input ChIP DNA from a mixture S. cerevisiae (MS282) and C. glabrata (MS99). MS282 was treated with rapamycin before collection.


title: 200917L34_exp200802_MS289_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS289) and C. glabrata (MS99). MS289 was treated with rapamycin before collection.


title: 200917L09_exp200803_MS282_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS281) and C. glabrata (MS99). MS281 was treated with rapamycin before collection.


title: 200917L30_exp200802_MS208_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS208) and C. glabrata (MS99). MS208 was treated with rapamycin before collection.


title: 200917L31_exp200802_MS209_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS209) and C. glabrata (MS99). MS209 was treated with rapamycin before collection.


title: 200808L23_exp200803_MS299_SCD-RAPA_anti-Rpb1

value: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS299) and C. glabrata (MS99). MS299 was treated with rapamycin before collection.


title: 200917L29_exp200802_MS207_SCD-RAPA_input

value: Input ChIP DNA from a mixture S. cerevisiae (MS207) and C. glabrata (MS99). MS207 was treated with rapamycin before collection.


title: 200917L40_exp200803_MS289_SCD-RAPA_input

value: Input ChIP DNA from a mixture S. cerevisiae (MS289) and C. glabrata (MS99). MS289 was treated with rapamycin before collection.


title: 200808L19_exp200803_MS207_SCD-RAPA_anti-Rpb1


title: 200808L22_exp200803_MS294_SCD-RAPA_anti-Rpb1

value: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS294) and C. glabrata (MS99). MS294 was treated with rapamycin before collection.


title: 200808L16_exp200802_MS282_SCD-RAPA_anti-Rpb1


title: 200808L21_exp200803_MS282_SCD-RAPA_anti-Rpb1


title: 200917L38_exp200803_MS294_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS294) and C. glabrata (MS99). MS294 was treated with rapamycin before collection.


title: 200808L10_exp200803_MS294_SCD-RAPA_input

value: Input ChIP DNA from a mixture S. cerevisiae (MS294) and C. glabrata (MS99). MS294 was treated with rapamycin before collection.


title: 200808L09_exp200803_MS282_SCD-RAPA_input


title: 200808L08_exp200803_MS281_SCD-RAPA_input

value: Input ChIP DNA from a mixture S. cerevisiae (MS281) and C. glabrata (MS99). MS281 was treated with rapamycin before collection.


title: 200917L03_exp200802_MS209_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS209) and C. glabrata (MS99). MS209 was treated with rapamycin before collection.


title: 200808L07_exp200803_MS207_SCD-RAPA_input


title: 200808L06_exp200802_MS289_SCD-RAPA_input


title: 200808L17_exp200802_MS295_SCD-RAPA_anti-Rpb1

value: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS295) and C. glabrata (MS99). MS295 was treated with rapamycin before collection.


title: 200808L13_exp200802_MS207_SCD-RAPA_anti-Rpb1

value: yeast


title: 200808L20_exp200803_MS281_SCD-RAPA_anti-Rpb1

value: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS281) and C. glabrata (MS99). MS281 was treated with rapamycin before collection.


title: 200808L02_exp200802_MS208_SCD-RAPA_input

value: Input ChIP DNA from a mixture S. cerevisiae (MS208) and C. glabrata (MS99). MS208 was treated with rapamycin before collection.


title: 200808L03_exp200802_MS209_SCD-RAPA_input

value: Input ChIP DNA from a mixture S. cerevisiae (MS209) and C. glabrata (MS99). MS209 was treated with rapamycin before collection.


title: 200808L01_exp200802_MS207_SCD-RAPA_input


title: 200808L18_exp200802_MS289_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS289) and C. glabrata (MS99). MS289 was treated with rapamycin before collection.


title: 200808L14_exp200802_MS208_SCD-RAPA_anti-Rpb1

value: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS208) and C. glabrata (MS99). MS208 was treated with rapamycin before collection.


title: 200808L12_exp200803_MS289_SCD-RAPA_input


title: 200526L44_exp200207_MS289_SCD-RAPA_input


title: 200526L70_exp200207_MS208_SCD-RAPA_anti-Rpb1


title: 200526L67_exp200207_MS207_SCD-RAPA_anti-Rpb1


title: 200808L11_exp200803_MS299_SCD-RAPA_input

value: Input ChIP DNA from a mixture S. cerevisiae (MS299) and C. glabrata (MS99). MS299 was treated with rapamycin before collection.


title: 200808L24_exp200803_MS289_SCD-RAPA_anti-Rpb1

value: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS289) and C. glabrata (MS99). MS289 was treated with rapamycin before collection.


title: 200808L05_exp200802_MS295_SCD-RAPA_input

value: Input ChIP DNA from a mixture S. cerevisiae (MS295) and C. glabrata (MS99). MS295 was treated with rapamycin before collection.


title: 200808L04_exp200802_MS282_SCD-RAPA_input


title: 200216L13_exp200207_MS207_SCD-RAPA_input


title: 200808L15_exp200802_MS209_SCD-RAPA_anti-Rpb1

value: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS209) and C. glabrata (MS99). MS209 was treated with rapamycin before collection.

value: anti-Rpb1 (8wG16; Millipore # 05-952-I)


title: 200216L38_exp200207_MS289_SCD-RAPA_anti-Rpb1


title: 200526L46_exp200207_MS208_SCD-RAPA_input


title: 200526L32_exp200120_MS289_SCD-RAPA_input


title: 200526L43_exp200207_MS207_SCD-RAPA_input


title: 200526L58_exp200120_MS208_SCD-RAPA_anti-Rpb1


title: 200526L56_exp200120_MS289_SCD-RAPA_anti-Rpb1


title: 200526L55_exp200120_MS207_SCD-RAPA_anti-Rpb1

value: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS207) and C. glabrata (MS99). MS207 was treated with rapamycin before collection.


title: 200216L14_exp200207_MS289_SCD-RAPA_input


title: 200216L88_exp200207_MS208_SCD-RAPA_input


title: 200526L68_exp200207_MS289_SCD-RAPA_anti-Rpb1


title: 200216L40_exp200207_MS208_SCD-RAPA_anti-Rpb1


title: 191015L39_exp190712_MS288_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS288) and C. glabrata (MS99). MS288 was treated with rapamycin before collection.


title: 200201L37_exp200120_MS207_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS207) and C. glabrata (MS99). MS207 was treated with rapamycin before collection.


title: 200201L38_exp200120_MS289_SCD-RAPA_anti-Rpb1


title: 191015L38_exp190712_MS287_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS287) and C. glabrata (MS99). MS287 was treated with rapamycin before collection.


title: 200201L40_exp200120_MS208_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS208) and C. glabrata (MS99). MS208 was treated with rapamycin before collection.


title: 191015L35_exp190712_MS209_SCD-RAPA_anti-Rpb1


title: 191015L05_exp190712_MS286_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS286) and C. glabrata (MS99). MS286 was treated with rapamycin before collection.


title: 191015L33_exp190712_MS207_SCD-RAPA_anti-Rpb1


title: 200216L37_exp200207_MS207_SCD-RAPA_anti-Rpb1


title: 191015L08_exp190712_MS289_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS281) and C. glabrata (MS99). MS281 was treated with rapamycin before collection.


title: 191020L13_exp190711_MS283_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS283) and C. glabrata (MS99). MS283 was treated with rapamycin before collection.


title: 191020L09_exp190711_MS207_SCD-RAPA_anti-Rpb1


title: 191015L03_exp190712_MS209_SCD-RAPA_input


title: 191020L04_exp190711_MS283_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS283) and C. glabrata (MS99). MS283 was treated with rapamycin before collection.


title: 191020L07_exp190711_MS285_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS285) and C. glabrata (MS99). MS285 was treated with rapamycin before collection.


title: 191020L15_exp190711_MS285_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS285) and C. glabrata (MS99). MS285 was treated with rapamycin before collection.


title: 191020L11_exp190711_MS281_SCD-RAPA_anti-Rpb1


title: 200526L31_exp200120_MS207_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS207) and C. glabrata (MS99). MS207 was treated with rapamycin before collection.


title: 191020L01_exp190711_MS207_SCD-RAPA_input

value: Input ChIP DNA from a mixture S. cerevisiae (MS207) and C. glabrata (MS99). MS207 was treated with rapamycin before collection.


title: 200224L78_exp200120_MS208_SCD-RAPA_input


title: 200526L34_exp200120_MS208_SCD-RAPA_input


title: 200224L76_exp200120_MS289_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS289) and C. glabrata (MS99). MS289 was treated with rapamycin before collection.


title: 200224L75_exp200120_MS207_SCD-RAPA_input


title: 190729L31_exp190712_MS288_SCD-RAPA_anti-Rpb1

value: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS288) and C. glabrata (MS99). MS288 was treated with rapamycin before collection.


title: 191015L37_exp190712_MS286_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS286) and C. glabrata (MS99). MS286 was treated with rapamycin before collection.


title: 191020L14_exp190711_MS284_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS284) and C. glabrata (MS99). MS284 was treated with rapamycin before collection.


title: 191015L36_exp190712_MS283_SCD-RAPA_anti-Rpb1

value: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS283) and C. glabrata (MS99). MS283 was treated with rapamycin before collection.


title: 190729L28_exp190712_MS283_SCD-RAPA_anti-Rpb1


title: 191015L40_exp190712_MS289_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS281) and C. glabrata (MS99). MS281 was treated with rapamycin before collection.


title: 191015L07_exp190712_MS288_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS288) and C. glabrata (MS99). MS288 was treated with rapamycin before collection.


title: 191015L06_exp190712_MS287_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS287) and C. glabrata (MS99). MS287 was treated with rapamycin before collection.


title: 190729L32_exp190712_MS289_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS289) and C. glabrata (MS99). MS289 was treated with rapamycin before collection.


title: 191015L04_exp190712_MS283_SCD-RAPA_input

value: Input ChIP DNA from a mixture S. cerevisiae (MS283) and C. glabrata (MS99). MS283 was treated with rapamycin before collection.


title: 191015L01_exp190712_MS207_SCD-RAPA_input


title: 190729L52_exp190712_MS207_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS207) and C. glabrata (MS99). MS207 was treated with rapamycin before collection.


title: 190729L23_exp190711_MS285_SCD-RAPA_anti-Rpb1

value: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS285) and C. glabrata (MS99). MS285 was treated with rapamycin before collection.


title: 191020L12_exp190711_MS282_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS282) and C. glabrata (MS99). MS282 was treated with rapamycin before collection.


title: 190729L22_exp190711_MS284_SCD-RAPA_anti-Rpb1

value: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS284) and C. glabrata (MS99). MS284 was treated with rapamycin before collection.


title: 190729L21_exp190711_MS283_SCD-RAPA_anti-Rpb1

value: yeast


title: 191020L04_exp190711_MS282_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS282) and C. glabrata (MS99). MS282 was treated with rapamycin before collection.


title: 190729L20_exp190711_MS282_SCD-RAPA_anti-Rpb1

value: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS282) and C. glabrata (MS99). MS282 was treated with rapamycin before collection.


title: 191020L16_exp190711_MS289_SCD-RAPA_anti-Rpb1


title: 191020L06_exp190711_MS284_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS284) and C. glabrata (MS99). MS284 was treated with rapamycin before collection.


title: 190729L49_exp190711_MS207_SCD-RAPA_anti-Rpb1


title: 190729L24_exp190711_MS289_SCD-RAPA_anti-Rpb1


title: 190722L40_exp190711_MS282_SCD-RAPA_anti-Rpb1


title: 190729L30_exp190712_MS287_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS287) and C. glabrata (MS287). MS209 was treated with rapamycin before collection.


title: 191020L03_exp190711_MS281_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS281) and C. glabrata (MS99). MS281 was treated with rapamycin before collection.


title: 190722L44_exp190711_MS289_SCD-RAPA_anti-Rpb1


title: 190729L29_exp190712_MS286_SCD-RAPA_anti-Rpb1

value: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS286) and C. glabrata (MS99). MS286 was treated with rapamycin before collection.


title: 190729L27_exp190712_MS209_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS209) and C. glabrata (MS99). MS209 was treated with rapamycin before collection.


title: 190729L19_exp190711_MS281_SCD-RAPA_anti-Rpb1

value: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS281) and C. glabrata (MS99). MS281 was treated with rapamycin before collection.


title: 190722L42_exp190711_MS284_SCD-RAPA_anti-Rpb1


title: 190722L41_exp190711_MS283_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS283) and C. glabrata (MS99). MS283 was treated with rapamycin before collection.


title: 190923L56_exp190712_MS286_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS286) and C. glabrata (MS99). MS286 was treated with rapamycin before collection.


title: 190923L57_exp190712_MS287_SCD-RAPA_input

value: Input ChIP DNA from a mixture S. cerevisiae (MS287) and C. glabrata (MS99). MS287 was treated with rapamycin before collection.


title: 190722L11_exp190712_MS209_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS209) and C. glabrata (MS99). MS209 was treated with rapamycin before collection.


title: 190923L55_exp190712_MS283_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS283) and C. glabrata (MS99). MS283 was treated with rapamycin before collection.


title: 190923L58_exp190712_MS288_SCD-RAPA_input

value: Input ChIP DNA from a mixture S. cerevisiae (MS288) and C. glabrata (MS99). MS288 was treated with rapamycin before collection.


title: 190722L37_exp190711_MS207_SCD-RAPA_anti-Rpb1


title: 190722L05_exp190711_MS283_SCD-RAPA_input


title: 190722L09_exp190712_MS207_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS207) and C. glabrata (MS99). MS207 was treated with rapamycin before collection.


title: 190722L12_exp190712_MS289_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS289) and C. glabrata (MS99). MS289 was treated with rapamycin before collection.


title: 190722L06_exp190711_MS284_SCD-RAPA_input

value: Input ChIP DNA from a mixture S. cerevisiae (MS284) and C. glabrata (MS99). MS284 was treated with rapamycin before collection.


title: 190722L43_exp190711_MS285_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS285) and C. glabrata (MS99). MS285 was treated with rapamycin before collection.


title: 190722L07_exp190711_MS285_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS285) and C. glabrata (MS99). MS285 was treated with rapamycin before collection.


title: 190722L08_exp190711_MS289_SCD-RAPA_input

value: Input ChIP DNA from a mixture S. cerevisiae (MS289) and C. glabrata (MS99). MS289 was treated with rapamycin before collection.


title: 190722L03_exp190711_MS281_SCD-RAPA_input

value: Input ChIP DNA from a mixture S. cerevisiae (MS281) and C. glabrata (MS99). MS281 was treated with rapamycin before collection.


title: 190722L39_exp190711_MS281_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS281) and C. glabrata (MS99). MS281 was treated with rapamycin before collection.

value: anti-Rpb1 (8wG16; Millipore # 05-952-I)


title: 200216L33_exp200108_MS284_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS284) and C. glabrata (MS99). MS284 was treated with rapamycin before collection.


title: 200216L35_exp200108_MS286_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS286) and C. glabrata (MS99). MS286 was treated with rapamycin before collection.


title: 200216L32_exp200108_MS289_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS289) and C. glabrata (MS99). MS289 was treated with rapamycin before collection.


title: 190722L04_exp190711_MS282_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS282) and C. glabrata (MS99). MS282 was treated with rapamycin before collection.


title: 200216L33_exp200108_MS282_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS282) and C. glabrata (MS99). MS282 was treated with rapamycin before collection.


title: 200216L30_exp200112_MS287_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS287) and C. glabrata (MS99). MS287 was treated with rapamycin before collection.


title: 200216L31_exp200108_MS207_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS207) and C. glabrata (MS99). MS207 was treated with rapamycin before collection.


title: 200216L28_exp200112_MS283_SCD-RAPA_anti-Rpb1


title: 200216L36_exp200108_MS288_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS288) and C. glabrata (MS99). MS288 was treated with rapamycin before collection.


title: 200216L29_exp200112_MS285_SCD-RAPA_anti-Rpb1


title: 200216L26_exp200112_MS289_SCD-RAPA_anti-Rpb1

value: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS289) and C. glabrata (MS99). MS289 was treated with rapamycin before collection.


title: 200216L25_exp200112_MS207_SCD-RAPA_anti-Rpb1

value: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS207) and C. glabrata (MS99). MS207 was treated with rapamycin before collection.


title: 200216L12_exp200108_MS288_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS288) and C. glabrata (MS99). MS288 was treated with rapamycin before collection.


title: 200216L27_exp200112_MS281_SCD-RAPA_anti-Rpb1


title: 200216L11_exp200108_MS286_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS286) and C. glabrata (MS99). MS286 was treated with rapamycin before collection.


title: 200216L08_exp200108_MS289_SCD-RAPA_input


title: 190722L01_exp190711_MS207_SCD-RAPA_input

value: Input ChIP DNA from a mixture S. cerevisiae (MS207) and C. glabrata (MS99). MS207 was treated with rapamycin before collection.


title: 200216L10_exp200108_MS284_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS284) and C. glabrata (MS99). MS284 was treated with rapamycin before collection.


title: 200216L06_exp200112_MS287_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS287) and C. glabrata (MS99). MS287 was treated with rapamycin before collection.


title: 200216L04_exp200112_MS283_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS283) and C. glabrata (MS99). MS283 was treated with rapamycin before collection.


title: 200216L07_exp200108_MS207_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS207) and C. glabrata (MS99). MS207 was treated with rapamycin before collection.


title: 200216L02_exp200112_MS289_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS289) and C. glabrata (MS99). MS289 was treated with rapamycin before collection.


title: 200201L36_exp200101B_MS288_SCD-RAPA_anti-Rpb1

value: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS288) and C. glabrata (MS99). MS288 was treated with rapamycin before collection.


title: 200216L01_exp200112_MS207_SCD-RAPA_input


title: 200216L05_exp200112_MS285_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS285) and C. glabrata (MS99). MS285 was treated with rapamycin before collection.


title: 200201L34_exp200101B_MS284_SCD-RAPA_anti-Rpb1


title: 200201L33_exp200101B_MS282_SCD-RAPA_anti-Rpb1


title: 200201L35_exp200101B_MS286_SCD-RAPA_anti-Rpb1


title: 200201L30_exp200101A_MS287_SCD-RAPA_anti-Rpb1

value: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS287) and C. glabrata (MS99). MS287 was treated with rapamycin before collection.


title: 200201L31_exp200101B_MS207_SCD-RAPA_anti-Rpb1


title: 200201L28_exp200101A_MS283_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS283) and C. glabrata (MS99). MS283 was treated with rapamycin before collection.


title: 200201L29_exp200101A_MS285_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS285) and C. glabrata (MS99). MS285 was treated with rapamycin before collection.


title: 200201L32_exp200101B_MS289_SCD-RAPA_anti-Rpb1


title: 200201L27_exp200101A_MS281_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS281) and C. glabrata (MS99). MS281 was treated with rapamycin before collection.


title: 200201L26_exp200101A_MS289_SCD-RAPA_anti-Rpb1

description: Eluted Rpb1 ChIP DNA from a mixture S. cerevisiae (MS289) and C. glabrata (MS99). MS289 was treated with rapamycin before collection.


title: 200201L25_exp200101A_MS207_SCD-RAPA_anti-Rpb1


title: 200224L73_exp200101B_MS286_SCD-RAPA_input

value: Input ChIP DNA from a mixture S. cerevisiae (MS286) and C. glabrata (MS99). MS286 was treated with rapamycin before collection.


title: 200216L09_exp200108_MS282_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS282) and C. glabrata (MS99). MS282 was treated with rapamycin before collection.


title: 200224L72_exp200101B_MS284_SCD-RAPA_input


title: 200224L71_exp200101B_MS282_SCD-RAPA_input

value: Input ChIP DNA from a mixture S. cerevisiae (MS282) and C. glabrata (MS99). MS282 was treated with rapamycin before collection.


title: 200224L69_exp200101B_MS207_SCD-RAPA_input

value: yeast


title: 200224L74_exp200101B_MS288_SCD-RAPA_input


title: 200224L68_exp200101A_MS287_SCD-RAPA_input


title: 200216L03_exp200112_MS281_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS281) and C. glabrata (MS99). MS281 was treated with rapamycin before collection.


title: 200224L67_exp200101A_MS285_SCD-RAPA_input

value: Input ChIP DNA from a mixture S. cerevisiae (MS285) and C. glabrata (MS99). MS285 was treated with rapamycin before collection.


title: 190204L42_exp180712_MS63_SCGE-30nM-BE_anti-H4K16ac

description: Eluted H4K16ac ChIP DNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 30nM beta-estradiol.

value: anti-H4K16ac (Millipore #07-329)


title: 200224L66_exp200101A_MS283_SCD-RAPA_input

value: Input ChIP DNA from a mixture S. cerevisiae (MS283) and C. glabrata (MS99). MS283 was treated with rapamycin before collection.


title: 200224L65_exp200101A_MS281_SCD-RAPA_input


title: 200224L64_exp200101A_MS289_SCD-RAPA_input


title: 200224L63_exp200101A_MS207_SCD-RAPA_input


title: 190204L41_exp180712_MS63_SCGE-10nM-BE_anti-H4K16ac

description: Eluted H4K16ac ChIP DNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 10nM beta-estradiol.


title: 190204L40_exp180712_MS63_SCGE-00nM-BE_anti-H4K16ac

description: Eluted H4K16ac ChIP DNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99).


title: 190204L39_exp180712_MS81_SCGE_anti-H4K16ac

description: Eluted H4K16ac ChIP DNA from a mixture S. cerevisiae (MS81) and C. glabrata (MS99).


title: 190204L38_exp180712_MS80_SCGE_anti-H4K16ac

description: Eluted H4K16ac ChIP DNA from a mixture S. cerevisiae (MS80) and C. glabrata (MS99).


title: 190204L37_exp180712_MS01_SCGE_anti-H4K16ac

description: Eluted H4K16ac ChIP DNA from a mixture S. cerevisiae (MS01) and C. glabrata (MS99).


title: 190204L36_exp180712_MS63_SCGE-30nM-BE_anti-H3K27ac

description: Eluted H3K27ac ChIP DNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 30nM beta-estradiol.

value: anti-H3K27ac (Millipore #07-360)


title: 190204L34_exp180712_MS63_SCGE-00nM-BE_anti-H3K27ac

description: Eluted H3K27ac ChIP DNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99).


title: 190204L32_exp180712_MS80_SCGE_anti-H3K27ac

description: Eluted H3K27ac ChIP DNA from a mixture S. cerevisiae (MS80) and C. glabrata (MS99).


title: 190204L33_exp180712_MS81_SCGE_anti-H3K27ac

description: Eluted H3K27ac ChIP DNA from a mixture S. cerevisiae (MS81) and C. glabrata (MS99).


title: 200224L70_exp200101B_MS289_SCD-RAPA_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS289) and C. glabrata (MS99). MS289 was treated with rapamycin before collection.


title: 190204L28_exp180712_MS63_SCGE-00nM-BE_anti-H3K18ac

description: Eluted H3K18ac ChIP DNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99).

value: anti-H3K18ac (Abcam #ab1191)


title: 190204L30_exp180712_MS63_SCGE-30nM-BE_anti-H3K18ac

description: Eluted H3K18ac ChIP DNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 30nM beta-estradiol.


title: 190204L31_exp180712_MS01_SCGE_anti-H3K27ac

description: Eluted H3K27ac ChIP DNA from a mixture S. cerevisiae (MS01) and C. glabrata (MS99).


title: 190204L29_exp180712_MS63_SCGE-10nM-BE_anti-H3K18ac

description: Eluted H3K18ac ChIP DNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 10nM beta-estradiol.


title: 190204L26_exp180712_MS80_SCGE_anti-H3K18ac

description: Eluted H3K18ac ChIP DNA from a mixture S. cerevisiae (MS80) and C. glabrata (MS99).


title: 190204L25_exp180712_MS01_SCGE_anti-H3K18ac

description: Eluted H3K18ac ChIP DNA from a mixture S. cerevisiae (MS01) and C. glabrata (MS99).


title: 190204L18_exp180712_MS63_SCGE-30nM-BE_anti-H4

description: Eluted H4 ChIP DNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 30nM beta-estradiol.

value: anti-H4 (Abcam #ab10158)


title: 190204L16_exp180712_MS63_SCGE-00nM-BE_anti-H4

description: Eluted H4 ChIP DNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99).


title: 190204L15_exp180711_MS81_SCGE_anti-H4

description: Eluted H4 ChIP DNA from a mixture S. cerevisiae (MS81) and C. glabrata (MS99).


title: 190204L17_exp180712_MS63_SCGE-10nM-BE_anti-H4

description: Eluted H4 ChIP DNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 10nM beta-estradiol.


title: 190204L14_exp180711_MS80_SCGE_anti-H4

description: Eluted H4 ChIP DNA from a mixture S. cerevisiae (MS80) and C. glabrata (MS99).


title: 190204L12_exp180712_MS63_SCGE-30nM-BE_anti-H3

description: Eluted H3 ChIP DNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 30nM beta-estradiol.

value: anti-H3 (Abcam #ab1791)


title: 190204L35_exp180712_MS63_SCGE-10nM-BE_anti-H3K27ac

description: Eluted H3K27ac ChIP DNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 10nM beta-estradiol.


title: 190204L09_exp180711_MS81_SCGE_anti-H3

description: Eluted H3 ChIP DNA from a mixture S. cerevisiae (MS81) and C. glabrata (MS99).


title: 190204L08_exp180711_MS80_SCGE_anti-H3

description: Eluted H3 ChIP DNA from a mixture S. cerevisiae (MS80) and C. glabrata (MS99).


title: 190204L11_exp180712_MS63_SCGE-10nM-BE_anti-H3

description: Eluted H3 ChIP DNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 10nM beta-estradiol.


title: 190204L07_exp180711_MS01_SCGE_anti-H3

description: Eluted H3 ChIP DNA from a mixture S. cerevisiae (MS01) and C. glabrata (MS99).


title: 190204L13_exp180711_MS01_SCGE_anti-H4

description: Eluted H4 ChIP DNA from a mixture S. cerevisiae (MS01) and C. glabrata (MS99).


title: 190204L10_exp180712_MS63_SCGE-00nM-BE_anti-H3

description: Eluted H3 ChIP DNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99).


title: 181105L06-R_exp180712_MS63_SCGE-30nM-BE_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 30nM beta-estradiol.


title: 181105L04-R_exp180712_MS63_SCGE-00nM-BE_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99).


title: 181105L05-R_exp180712_MS63_SCGE-10nM-BE_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 10nM beta-estradiol.


title: 181105L03-R_exp180711_MS81_SCGE_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS81) and C. glabrata (MS99).


title: 190204L27_exp180712_MS81_SCGE_anti-H3K18ac

description: Eluted H3K18ac ChIP DNA from a mixture S. cerevisiae (MS81) and C. glabrata (MS99).


title: 181105L02-R_exp180711_MS80_SCGE_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS80) and C. glabrata (MS99).


title: 181105L01-R_exp180711_MS01_SCGE_input

description: Input ChIP DNA from a mixture S. cerevisiae (MS01) and C. glabrata (MS99).


title: 181105L72_exp180712_MS63_SCGE-30nM-BE_anti-H3K36me3

description: Eluted H3K36me3 ChIP DNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 30nM beta-estradiol.

value: anti-H3K36me3 (Abcam #ab9050)


title: 181105L70_exp180712_MS63_SCGE-00nM-BE_anti-H3K36me3

description: Eluted H3K36me3 ChIP DNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99).


title: 181105L71_exp180712_MS63_SCGE-10nM-BE_anti-H3K36me3

description: Eluted H3K36me3 ChIP DNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 10nM beta-estradiol.


title: 181105L65_exp180712_MS63_SCGE-10nM-BE_anti-H3K36me2

description: Eluted H3K36me2 ChIP DNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 10nM beta-estradiol.

value: anti-H3K36me2 (Abcam #ab9049)


title: 181105L67_exp180711_MS01_SCGE_anti-H3K36me3

description: Eluted H3K36me3 ChIP DNA from a mixture S. cerevisiae (MS01) and C. glabrata (MS99).


title: 181105L68_exp180711_MS80_SCGE_anti-H3K36me3

description: Eluted H3K36me3 ChIP DNA from a mixture S. cerevisiae (MS80) and C. glabrata (MS99).


title: 181105L66_exp180712_MS63_SCGE-30nM-BE_anti-H3K36me2

description: Eluted H3K36me2 ChIP DNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 30nM beta-estradiol.


title: 181105L69_exp180711_MS81_SCGE_anti-H3K36me3

description: Eluted H3K36me3 ChIP DNA from a mixture S. cerevisiae (MS81) and C. glabrata (MS99).


title: 181105L63_exp180711_MS81_SCGE_anti-H3K36me2

description: Eluted H3K36me2 ChIP DNA from a mixture S. cerevisiae (MS81) and C. glabrata (MS99).


title: 181105L62_exp180711_MS80_SCGE_anti-H3K36me2

description: Eluted H3K36me2 ChIP DNA from a mixture S. cerevisiae (MS80) and C. glabrata (MS99).


title: 181105L64_exp180712_MS63_SCGE-00nM-BE_anti-H3K36me2

description: Eluted H3K36me2 ChIP DNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99).


title: 181105L61_exp180711_MS01_SCGE_anti-H3K36me2

description: Eluted H3K36me2 ChIP DNA from a mixture S. cerevisiae (MS01) and C. glabrata (MS99).


title: 181105L60_exp180712_MS63_SCGE-30nM-BE_anti-H3K79me3

description: Eluted H3K79me3 ChIP DNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 30nM beta-estradiol.

value: anti-H3K79me3 (Abcam #ab2621)


title: 181105L59_exp180712_MS63_SCGE-10nM-BE_anti-H3K79me3

description: Eluted H3K79me3 ChIP DNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 10nM beta-estradiol.


title: 181105L58_exp180712_MS63_SCGE-00nM-BE_anti-H3K79me3

description: Eluted H3K79me3 ChIP DNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99).


title: 181105L57_exp180711_MS81_SCGE_anti-H3K79me3

description: Eluted H3K79me3 ChIP DNA from a mixture S. cerevisiae (MS81) and C. glabrata (MS99).


title: 181105L55_exp180711_MS01_SCGE_anti-H3K79me3

description: Eluted H3K79me3 ChIP DNA from a mixture S. cerevisiae (MS01) and C. glabrata (MS99).


title: 181105L52_exp180712_MS63_SCGE-00nM-BE_anti-H3K79me2

description: Eluted H3K79me2 ChIP DNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99).

value: anti-H3K79me2 (Abcam #ab3594)


title: 181105L51_exp180711_MS81_SCGE_anti-H3K79me2

description: Eluted H3K79me2 ChIP DNA from a mixture S. cerevisiae (MS81) and C. glabrata (MS99).


title: 181105L50_exp180711_MS80_SCGE_anti-H3K79me2

description: Eluted H3K79me2 ChIP DNA from a mixture S. cerevisiae (MS80) and C. glabrata (MS99).


title: 181105L53_exp180712_MS63_SCGE-10nM-BE_anti-H3K79me2

description: Eluted H3K79me2 ChIP DNA from a mixture S. cerevisiae (MS63) and C. glabrata (MS99). MS63 was cultured in the prescence of 10nM beta-estradiol.


title: 181105L56_exp180711_MS80_SCGE_anti-H3K79me3

description: Eluted H3K79me3 ChIP DNA from a mixture S. cerevisiae (MS80) and C. glabrata (MS99).