title: 3805_OM1A_3-5-E1-I10


title: 3805_OM1A_3-5-E1-D21

status: Public on Dec 14 2023

type: SRA

city: La Jolla

first: Joseph

middle: R

last: Ecker

state: CA

address: 10010 North Torrey Pines Road

department: Genomic Analysis Laboratory

country: USA

institute: HHMI-Salk-Institute

library_source: genomic

data_processing:
Reads trimmed with Cutadapt for adapters and low quality bases (Phred score >= 20)
The resulting files are mapped to in-silico bisulfite converted hg38 reference genome using Hisat-3n.
The raw BAM files were filtered using samtools to keep only uniquely mapped reads and use Picard to remove PCR duplicates.
From the filtered BAM file, DNA reads are selected by read-level mCH base coverage >= 3 and read-level mCH fraction < 0.5, selected reads will be processed by ALLCools package to produce ALLC files (allcools allc-to-bam function). The ALLC files are Tab-delimited files describing the quantity of unmethylated and methylated cytosine base calls.
Trimmed reads will also be mapped by Hisat2 to map to hg38 reference genome with GENCODE human v37 gene annotation. RNA reads are selected by read-level mCH base coverage >= 3 and read-level mCH fraction > 0.9, selected reads will be processed by featureCount to quantify gene level (exon + intron) RNA counts.
Assembly: hg38
Supplementary files format and content: tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls. Columns are: 1. Chromosome, 2. Position, 3. Strand, 4. Cytosin Context, 5. methylated base call, 6. total mapped base call, 7. not used, place holder column.
Library strategy: snmCT-seq

molecule: genomic DNA

organism: Homo sapiens

source_name: OM1A human BA46

extract_protocol: Library prepared following protocol discribed in Luo, Chongyuan, Hanqing Liu, Fangming Xie, Ethan J. Armand, Kimberly Siletti, Trygve E. Bakken, Rongxin Fang, et al. 2019. “Single Nucleus Multi-Omics Links Human Cortical Cell Regulatory Genome Diversity to Disease Risk Variants.” bioRxiv. https://doi.org/10.1101/2019.12.11.873398. https://www.protocols.io/view/snmcat-v1-bwubpesn

tag: brain region

value: Dorsolateral Prefrontal cortex (Brodmann area 46)

tag: age

tag: Sex

value: Male

tag: molecule subtype

value: nuclear RNA + genomic DNA


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title: 4634_OM2A_8-3-I11-G18


title: 4634_OM2A_8-3-I11-G6


title: 4634_OM2A_8-3-I11-I5


title: 4634_OM2A_8-3-I11-G5


title: 4634_OM2A_8-3-I11-H6


title: 4634_OM2A_8-3-I11-H17


title: 4634_OM2A_8-3-I11-H18


title: 4634_OM2A_8-3-I11-G17


title: 4634_OM2A_8-3-I11-F6


title: 4634_OM2A_8-3-I11-F5


title: 4634_OM2A_8-3-I11-F18


title: 4634_OM2A_8-3-I11-J17


title: 4634_OM2A_8-3-I11-H5


title: 4634_OM2A_8-3-I11-E17


title: 4634_OM2A_8-3-I11-D6


title: 4634_OM2A_8-3-I11-E5


title: 4634_OM2A_8-3-I11-E18


title: 4634_OM2A_8-3-I11-E6


title: 4634_OM2A_8-3-I11-F17


title: 4634_OM2A_8-3-I11-D5


title: 4634_OM2A_8-3-I11-D18


title: 4634_OM2A_8-3-I11-D17


title: 4634_OM2A_8-3-I11-C6


title: 4634_OM2A_8-3-I11-C5


title: 4634_OM2A_8-3-I11-C18


title: 4634_OM2A_8-3-I11-B17


title: 4634_OM2A_8-3-I11-B18


title: 4634_OM2A_8-3-I11-B5


title: 4634_OM2A_8-3-I11-B6


title: 4634_OM2A_8-3-I11-C17


title: 4634_OM2A_8-3-I11-A6


title: 4634_OM2A_8-2-I11-P16


title: 4634_OM2A_8-2-I11-P3


title: 4634_OM2A_8-2-I11-P4


title: 4634_OM2A_8-3-I11-A18


title: 4634_OM2A_8-2-I11-P15


title: 4634_OM2A_8-3-I11-A17


title: 4634_OM2A_8-3-I11-A5


title: 4634_OM2A_8-2-I11-O16


title: 4634_OM2A_8-2-I11-O15


title: 4634_OM2A_8-2-I11-O3


title: 4634_OM2A_8-2-I11-N4


title: 4634_OM2A_8-2-I11-M16


title: 4634_OM2A_8-2-I11-N16


title: 4634_OM2A_8-2-I11-M3


title: 4634_OM2A_8-2-I11-M15


title: 4634_OM2A_8-2-I11-N3


title: 4634_OM2A_8-2-I11-N15


title: 4634_OM2A_8-2-I11-M4


title: 4634_OM2A_8-2-I11-L4


title: 4634_OM2A_8-2-I11-K4


title: 4634_OM2A_8-2-I11-L3


title: 4634_OM2A_8-2-I11-L16


title: 4634_OM2A_8-2-I11-K15


title: 4634_OM2A_8-2-I11-L15


title: 4634_OM2A_8-2-I11-J4


title: 4634_OM2A_8-2-I11-J3


title: 4634_OM2A_8-2-I11-K16


title: 4634_OM2A_8-2-I11-I4


title: 4634_OM2A_8-2-I11-O4


title: 4634_OM2A_8-2-I11-J15


title: 4634_OM2A_8-2-I11-I15


title: 4634_OM2A_8-2-I11-I16


title: 4634_OM2A_8-2-I11-J16


title: 4634_OM2A_8-2-I11-H4


title: 4634_OM2A_8-2-I11-I3


title: 4634_OM2A_8-2-I11-H16


title: 4634_OM2A_8-2-I11-H15


title: 4634_OM2A_8-2-I11-H3


title: 4634_OM2A_8-2-I11-G4


title: 4634_OM2A_8-2-I11-G3


title: 4634_OM2A_8-2-I11-F4


title: 4634_OM2A_8-2-I11-G16


title: 4634_OM2A_8-2-I11-K3


title: 4634_OM2A_8-2-I11-G15


title: 4634_OM2A_8-2-I11-F3


title: 4634_OM2A_8-2-I11-E3


title: 4634_OM2A_8-2-I11-E4


title: 4634_OM2A_8-2-I11-F15


title: 4634_OM2A_8-2-I11-D16


title: 4634_OM2A_8-2-I11-E15


title: 4634_OM2A_8-2-I11-C3


title: 4634_OM2A_8-2-I11-D4


title: 4634_OM2A_8-2-I11-D3


title: 4634_OM2A_8-2-I11-C16


title: 4634_OM2A_8-2-I11-D15


title: 4634_OM2A_8-2-I11-A4


title: 4634_OM2A_8-2-I11-C15


title: 4634_OM2A_8-2-I11-B4


title: 4634_OM2A_8-2-I11-C4


title: 4634_OM2A_8-2-I11-B3


title: 4634_OM2A_8-2-I11-F16


title: 4634_OM2A_8-1-I11-P2


title: 4634_OM2A_8-2-I11-A3


title: 4634_OM2A_8-2-I11-A15


title: 4634_OM2A_8-1-I11-P14


title: 4634_OM2A_8-1-I11-P1


title: 4634_OM2A_8-2-I11-E16


title: 4634_OM2A_8-1-I11-P13


title: 4634_OM2A_8-1-I11-O2


title: 4634_OM2A_8-1-I11-O14


title: 4634_OM2A_8-1-I11-O1


title: 4634_OM2A_8-1-I11-O13


title: 4634_OM2A_8-1-I11-N1


title: 4634_OM2A_8-1-I11-N2


title: 4634_OM2A_8-2-I11-B16


title: 4634_OM2A_8-1-I11-N14


title: 4634_OM2A_8-2-I11-B15


title: 4634_OM2A_8-1-I11-N13


title: 4634_OM2A_8-1-I11-M13


title: 4634_OM2A_8-1-I11-M1


title: 4634_OM2A_8-1-I11-M2


title: 4634_OM2A_8-1-I11-L2


title: 4634_OM2A_8-1-I11-L14


title: 4634_OM2A_8-2-I11-A16


title: 4634_OM2A_8-1-I11-K2


title: 4634_OM2A_8-1-I11-L1


title: 4634_OM2A_8-1-I11-L13


title: 4634_OM2A_8-1-I11-K1


title: 4634_OM2A_8-1-I11-K13


title: 4634_OM2A_8-1-I11-K14


title: 4634_OM2A_8-1-I11-J2


title: 4634_OM2A_8-1-I11-J14


title: 4634_OM2A_8-1-I11-J13


title: 4634_OM2A_8-1-I11-M14


title: 4634_OM2A_8-1-I11-I14


title: 4634_OM2A_8-1-I11-I2


title: 4634_OM2A_8-1-I11-H2


title: 4634_OM2A_8-1-I11-I13


title: 4634_OM2A_8-1-I11-H1


title: 4634_OM2A_8-1-I11-H13


title: 4634_OM2A_8-1-I11-G13


title: 4634_OM2A_8-1-I11-F2


title: 4634_OM2A_8-1-I11-H14


title: 4634_OM2A_8-1-I11-E13


title: 4634_OM2A_8-1-I11-G2


title: 4634_OM2A_8-1-I11-G1


title: 4634_OM2A_8-1-I11-E14


title: 4634_OM2A_8-1-I11-D2


title: 4634_OM2A_8-1-I11-D1


title: 4634_OM2A_8-1-I11-F1


title: 4634_OM2A_8-1-I11-J1


title: 4634_OM2A_8-1-I11-C1


title: 4634_OM2A_8-1-I11-D13


title: 4634_OM2A_8-1-I11-I1


title: 4634_OM2A_8-1-I11-F14


title: 4634_OM2A_8-1-I11-F13


title: 4634_OM2A_8-1-I11-B14


title: 4634_OM2A_8-1-I11-B2


title: 4634_OM2A_8-1-I11-E1


title: 4634_OM2A_8-1-I11-E2


title: 4634_OM2A_8-1-I11-D14


title: 4634_OM2A_7-6-G11-P24


title: 4634_OM2A_7-6-G11-P23


title: 4634_OM2A_8-1-I11-G14


title: 4634_OM2A_8-1-I11-C2


title: 4634_OM2A_8-1-I11-C14


title: 4634_OM2A_7-6-G11-P11


title: 4634_OM2A_8-1-I11-C13


title: 4634_OM2A_7-6-G11-O12


title: 4634_OM2A_8-1-I11-A2


title: 4634_OM2A_8-1-I11-B13


title: 4634_OM2A_7-6-G11-O11


title: 4634_OM2A_8-1-I11-A1


title: 4634_OM2A_7-6-G11-N24


title: 4634_OM2A_7-6-G11-N11


title: 4634_OM2A_8-1-I11-A13


title: 4634_OM2A_8-1-I11-A14


title: 4634_OM2A_7-6-G11-L23


title: 4634_OM2A_7-6-G11-M11


title: 4634_OM2A_7-6-G11-M12


title: 4634_OM2A_7-6-G11-O23


title: 4634_OM2A_8-1-I11-B1


title: 4634_OM2A_7-6-G11-N23


title: 4634_OM2A_7-6-G11-M24


title: 4634_OM2A_7-6-G11-K12


title: 4634_OM2A_7-6-G11-K23


title: 4634_OM2A_7-6-G11-O24


title: 4634_OM2A_7-6-G11-K11


title: 4634_OM2A_7-6-G11-J24


title: 4634_OM2A_7-6-G11-J12


title: 4634_OM2A_7-6-G11-L24


title: 4634_OM2A_7-6-G11-L11


title: 4634_OM2A_7-6-G11-M23


title: 4634_OM2A_7-6-G11-P12


title: 4634_OM2A_7-6-G11-K24


title: 4634_OM2A_7-6-G11-I23


title: 4634_OM2A_7-6-G11-I12


title: 4634_OM2A_7-6-G11-H23


title: 4634_OM2A_7-6-G11-N12


title: 4634_OM2A_7-6-G11-G23


title: 4634_OM2A_7-6-G11-I11


title: 4634_OM2A_7-6-G11-L12


title: 4634_OM2A_7-6-G11-G11


title: 4634_OM2A_7-6-G11-I24


title: 4634_OM2A_7-6-G11-F24


title: 4634_OM2A_7-6-G11-F12


title: 4634_OM2A_7-6-G11-F23


title: 4634_OM2A_7-6-G11-J11


title: 4634_OM2A_7-6-G11-E23


title: 4634_OM2A_7-6-G11-F11


title: 4634_OM2A_7-6-G11-H12


title: 4634_OM2A_7-6-G11-G24


title: 4634_OM2A_7-6-G11-D23


title: 4634_OM2A_7-6-G11-D24


title: 4634_OM2A_7-6-G11-E12


title: 4634_OM2A_7-6-G11-D12


title: 4634_OM2A_7-6-G11-J23


title: 4634_OM2A_7-6-G11-C24


title: 4634_OM2A_7-6-G11-E11


title: 4634_OM2A_7-6-G11-H11


title: 4634_OM2A_7-6-G11-H24


title: 4634_OM2A_7-6-G11-C11


title: 4634_OM2A_7-6-G11-D11


title: 4634_OM2A_7-6-G11-B24


title: 4634_OM2A_7-6-G11-E24


title: 4634_OM2A_7-6-G11-C23


title: 4634_OM2A_7-6-G11-B23


title: 4634_OM2A_7-6-G11-B11


title: 4634_OM2A_7-6-G11-A12


title: 4634_OM2A_7-6-G11-A23


title: 4634_OM2A_7-6-G11-A11


title: 4634_OM2A_7-6-G11-C12


title: 4634_OM2A_7-6-G11-A24


title: 4634_OM2A_7-6-G11-G12


title: 4634_OM2A_7-5-G11-P9


title: 4634_OM2A_7-5-G11-P22


title: 4634_OM2A_7-5-G11-P21


title: 4634_OM2A_7-5-G11-P10


title: 4634_OM2A_7-5-G11-O9


title: 4634_OM2A_7-5-G11-O10


title: 36_ANNUBP

type: RNA

city: Indianapolis

first: Steven

last: Rhodes

state: IN

address: 1044 W. Walnut St.; Bldg R4, Rm 325

institute: Indiana University

hyb_protocol: Probes and target transcripts were hybridized for 20 hours at 65°C per the manufacturer's recommendations.

data_processing:
Hybridized samples were loaded into a nCounter cartridge and quantified on the nCounter® Sprint Profiler
Quality control, normalization, differential expression and pathway analysis were performed using nSolver Advanced Analysis Software (version 4.0, NanoString Technologies, Inc.) .

molecule: polyA RNA

source_name: FFPE tumor tissue

extract_protocol:
Formalin fixed paraffin embedded (FFPE) tissues were microdissected from 5 µm thick sections on freshly cut slides.
Total RNA was extracted using a RNeasy FFPE Kit (Qiagen). RNA concentration and fragmentation index were quantified on an Agilent bioanalyzer.
Fragmentation index (percent greater than 200 bp) was used to normalize the loading of 50 ng total input RNA in a final volume of 5 µL with the capture probe set.

tag: subject id

tag: diagnosis

value: ANNUBP

tag: age at surgery

tag: race/ethnicity

value: White, not Hispanic or Latino

tag: nf1 diagnosis

value: Yes

tag: family history of nf1

value: No

tag: location

value: Mediastinum/right lung

tag: biopsy vs surgical resection

value: Resection

tag: procedure (1st, 2nd, etc.)

tag: primary vs metastatic

value: Primary

tag: prior chemotherapy

tag: prior radiation (dose if known)

tag: contiguous with mpnst


title: 4634_OM2A_7-5-G11-N22

tag: brain region


title: 4634_OM2A_7-5-G11-O22

tag: age


title: 4634_OM2A_7-5-G11-N9

tag: Sex


title: 4634_OM2A_7-5-G11-O21

tag: molecule subtype


title: 4634_OM2A_7-5-G11-N10


title: 32_NF with atypia

tag: subject id

value: NF with atypia

value: Black or African American

value: Left thigh/hip


title: 33_MPNST

tag: diagnosis

value: MPNST (High-Grade)

value: Hispanic or Latino

value: Right axilla/shoulder/chest wall

value: Ifos/Doxo

value: Yes (45 Gy)


title: 34_ANNUBP

tag: age at surgery


title: 4634_OM2A_7-6-G11-B12

value: Dorsolateral Prefrontal cortex (Brodmann area 46)

value: Male

value: nuclear RNA + genomic DNA


title: 31_ANNUBP

value: ANNUBP

value: Female

tag: race/ethnicity


title: 4634_OM2A_7-5-G11-M9


title: 4634_OM2A_7-5-G11-N21


title: 30_NF

value: NF

tag: nf1 diagnosis

value: Yes

value: No


title: 4634_OM2A_7-5-G11-M22


title: 28_PNF

value: PNF

tag: family history of nf1

value: Right neck/occipital

value: Resection

value: Primary

value: mTOR inhibitor, Sunitinib malate


title: 29_NF with atypia

value: NF with atypia

value: Black or African American

tag: location


title: 4634_OM2A_7-5-G11-M21


title: 4634_OM2A_7-5-G11-M10


title: 27_MPNST

value: MPNST (High-Grade)

value: Right parotid/mandibular

tag: biopsy vs surgical resection


title: 4634_OM2A_7-5-G11-L22

value: Dorsolateral Prefrontal cortex (Brodmann area 46)


title: 4634_OM2A_7-5-G11-L9

value: Male


title: 26_PNF

tag: procedure (1st, 2nd, etc.)


title: 35_PNF

value: Hispanic or Latino

value: Right axilla/shoulder/chest wall

tag: primary vs metastatic

value: Ifos/Doxo

value: Yes (45 Gy)


title: 4634_OM2A_7-5-G11-L21

value: nuclear RNA + genomic DNA


title: 25_ANNUBP

value: ANNUBP

value: Female

value: Yes

value: No

tag: prior chemotherapy


title: 24_NF with atypia

value: White, not Hispanic or Latino

value: Left arm

value: Resection

value: Primary

tag: prior radiation (dose if known)


title: 23_PNF

value: PNF

tag: contiguous with mpnst


title: 4634_OM2A_7-5-G11-L10

tag: brain region


title: 21_PNF

value: Paraspinal C7-T4

value: Biopsy

value: Sunitinib malate


title: 4634_OM2A_7-5-G11-K9

value: Dorsolateral Prefrontal cortex (Brodmann area 46)

tag: age

value: Male


title: 22_ANNUBP

tag: Sex

value: Right mediastinum


title: 4634_OM2A_7-5-G11-K21

tag: molecule subtype


title: 4634_OM2A_7-5-G11-K22


title: 20_PNF

tag: subject id

value: Native Hawaiian or Pacific Islander

value: Right upper eyelid


title: 18_NF with atypia

tag: diagnosis

value: NF with atypia

value: Lumbar spine


title: 19_ANNUBP

value: ANNUBP

tag: age at surgery

value: Left distal halux


title: 17_NF

value: NF

value: Female

tag: race/ethnicity

value: White, not Hispanic or Latino

value: Yes

value: No


title: 4634_OM2A_7-5-G11-J22

value: nuclear RNA + genomic DNA


title: 4634_OM2A_7-5-G11-J10


title: 4634_OM2A_7-5-G11-K10


title: 4634_OM2A_7-5-G11-J9


title: 4634_OM2A_7-5-G11-J21


title: 4634_OM2A_7-5-G11-I9


title: 15_MPNST

value: MPNST (High-Grade)

value: Black or African American

tag: nf1 diagnosis

value: Left shoulder

value: Resection

value: Primary

value: Ifos/Doxo x4 cycles


title: 4634_OM2A_7-5-G11-I22

value: Dorsolateral Prefrontal cortex (Brodmann area 46)

value: Male


title: 14_NF with atypia

value: NF with atypia

tag: family history of nf1


title: 13_PNF

value: PNF

tag: location


title: 11_Cellular NF

value: Cellular NF

value: Right posterior auricular

tag: biopsy vs surgical resection


title: 4634_OM2A_7-5-G11-I10


title: 10_ANNUBP

value: ANNUBP

value: Female

value: Left pelvis

tag: procedure (1st, 2nd, etc.)


title: 4634_OM2A_7-5-G11-H9


title: 9_MPNST

value: Yes

value: Unknown

value: Left upper chest

tag: primary vs metastatic

value: No


title: 4634_OM2A_7-5-G11-H22

value: nuclear RNA + genomic DNA


title: 4634_OM2A_7-5-G11-H21


title: 8_MPNST

value: MPNST (High-Grade)

value: Black or African American

value: Left shoulder

value: Resection

tag: prior chemotherapy


title: 6_ANNUBP

value: Asian or Pacific Islander

value: Suspected

value: Back

value: Primary

tag: prior radiation (dose if known)


title: 5_NF with atypia

value: NF with atypia

value: White, not Hispanic or Latino

tag: contiguous with mpnst


title: 4634_OM2A_7-5-G11-G9

tag: brain region

value: Dorsolateral Prefrontal cortex (Brodmann area 46)

value: Male


title: 3_PNF

value: PNF

value: Female


title: 4634_OM2A_7-5-G11-G21

tag: age


title: 4634_OM2A_7-5-G11-H10

tag: Sex


title: 4_ANNUBP

value: ANNUBP

value: Yes

value: Right mediastinum

value: Sunitinib malate


title: 4634_OM2A_7-5-G11-G10

tag: molecule subtype

value: nuclear RNA + genomic DNA


title: 4634_OM2A_7-5-G11-G22


title: 1_PNF

tag: subject id

value: Black or African American

value: No

value: Left shoulder


title: 4634_OM2A_7-5-G11-F22


title: 2_PNF

tag: diagnosis

value: Resection


title: 4634_OM2A_7-5-G11-I21


title: 16_MPNST

value: MPNST (High-Grade)

tag: age at surgery

value: Left upper lung/pleura

value: Metastatic

value: Sorafenib, Ifos/Doxo, Ifos/Etop, Cisplatin, Rapamycin


title: Ax 7, SCFM, 6

city: Birmingham

first: Caitlin

middle: E

last: Billiot

state: Alabama

address: 1918 University Blvd, MCLM 818

department: Pulmonary, Allergy, and Critical Care Medicine

institute: University of Alabama at Birmingham

library_source: transcriptomic

data_processing:
Reads trimmed with TrimGalore! (v. 0.4.4) and Cutadapt (v. 1.9.1).
Aligned to published genome Ax NH44784-1996 using STAR aligner (v. 2.7.3a)
Read counts obtained via Subread FeatureCounts.
Differential expression analysis performed using DESeq2.
Pathway analysis performed using clusterProfiler (v. 4.4.1) with Gene Ontology and KEGG pathway databases.
Supplementary files format and content: Text file with  counts for each sample
Supplementary files format and content: CSV with differential expression data for each treatment group combined

molecule: total RNA

organism: Achromobacter xylosoxidans

source_name: clinical isolate Ax 7

growth_protocol: Achromobacter xylosoxidans overnight cultures were subcultured into LB or SCFM2 and grown for 5 hours until stationary phase was reached.

extract_protocol:
RNA was extracted using a standard Trizol protocol. Samples were DNase treated with NEB DNase.
Library construction was performed using Illumina's Stranded Total RNA Prep Ligation with Ribo-Zero Plus kit and 10bp IDT for Illumina indicies.

tag: cell line

value: clinical isolate Ax 7

tag: cell type

value: Bacterial

tag: genotype

value: WT

tag: treatment


title: 4634_OM2A_7-5-G11-F9

value: Dorsolateral Prefrontal cortex (Brodmann area 46)

value: Male


title: 4634_OM2A_7-5-G11-F21


title: 4634_OM2A_7-5-G11-F10


title: 4634_OM2A_7-5-G11-E9


title: Ax 7, SCFM, 2


title: 4634_OM2A_7-5-G11-E22


title: Ax 7, LB, 2

value: LB


title: 4634_OM2A_7-5-G11-E10


title: Ax 7, LB, 3


title: 4634_OM2A_7-5-G11-E21


title: Ax 7, LB, 1


title: 7_ANNUBP

value: ANNUBP

value: Female

tag: race/ethnicity

tag: nf1 diagnosis

value: Yes

tag: family history of nf1

tag: location

value: Right posterior auricular

tag: biopsy vs surgical resection

value: Primary


title: 4634_OM2A_7-5-G11-D9

value: nuclear RNA + genomic DNA


title: 4634_OM2A_7-5-G11-D22


title: 4634_OM2A_7-5-G11-C9


title: 4634_OM2A_7-5-G11-D21


title: 4634_OM2A_7-5-G11-D10


title: 4634_OM2A_7-5-G11-C22


title: Participant5_SD_CSF_24hrs

type: protein

city: St. Louis

first: Jinsheng

last: Yu

state: MO

address: 660 S. Euclid Ave.

department: Genetics

institute: Washington University School of Medicine

hyb_protocol: Hybridized overnight (19 hours) at 55 C as recommended by SomaLogic.

scan_protocol: SOMAmer matching probes printed onto Agilent slides.  After overnight hybridization slides were scanned on an Agilent Microarray SureScan Scanner model D.

data_processing:
Standard data processing method as per SomaLogic recommended methods.  Normalized and calibrated using kit control samples
quantile-normalization was applied on top of vendor-standardized and calibrated relative fluorescence unit (RFU, in log2 scale) and non-normalized and non-standardized raw data are provided in linear scale.

molecule: protein

source_name: CSF

label_protocol: Cy3 labelled SOMAmer.

extract_protocol: Intravenous lines were placed for longitudinal collection of blood. Blood samples were centrifuged to extract plasma. A lumbar catheter was placed for longitudinal CSF sampling.

tag: individual

tag: condition

value: sleep deprived

tag: time

tag: tissue

value: CSF


title: Ax 7, SCFM, 1


title: 4634_OM2A_7-5-G11-C21

tag: brain region

tag: age

tag: Sex

tag: molecule subtype


title: Participant3_SD_CSF_24hrs


title: 4634_OM2A_7-5-G11-C10


title: 4634_OM2A_7-5-G11-B9


title: Participant2_SD_CSF_24hrs


title: Participant1_SD_CSF_24hrs


title: 4634_OM2A_7-5-G11-B21


title: 4634_OM2A_7-5-G11-B10


title: 4634_OM2A_7-5-G11-B22


title: Participant5_SD_CSF_0hr


title: Participant3_SD_CSF_0hr


title: 4634_OM2A_7-5-G11-A9


title: Participant2_SD_CSF_0hr


title: Participant1_SD_CSF_0hr


title: 4634_OM2A_7-5-G11-A21


title: 4634_OM2A_7-5-G11-A22


title: Participant2_CTRL_CSF_24hrs

value: normal sleep


title: Participant3_CTRL_CSF_24hrs


title: Participant4_CTRL_CSF_24hrs


title: Participant5_CTRL_CSF_24hrs


title: 4634_OM2A_7-5-G11-A10


title: Participant1_CTRL_CSF_24hrs


title: 4634_OM2A_7-4-G11-P8


title: Participant4_SD_CSF_24hrs


title: 4634_OM2A_7-4-G11-P7


title: Participant4_SD_CSF_0hr


title: 4634_OM2A_7-4-G11-P20


title: Participant4_CTRL_CSF_0hr


title: Participant5_CTRL_CSF_0hr


title: 4634_OM2A_7-4-G11-P19


title: 4634_OM2A_7-4-G11-O8


title: 4634_OM2A_7-4-G11-O7


title: Participant3_CTRL_CSF_0hr


title: 4634_OM2A_7-4-G11-N8


title: Participant5_SD_plasma_24hrs

source_name: plasma

value: plasma


title: Participant2_CTRL_CSF_0hr


title: 4634_OM2A_7-4-G11-N7


title: Participant1_CTRL_CSF_0hr


title: Participant4_SD_plasma_24hrs


title: Participant1_SD_plasma_24hrs


title: Participant3_SD_plasma_24hrs


title: Participant5_SD_plasma_0hr


title: Participant2_SD_plasma_0hr


title: Participant3_SD_plasma_0hr


title: Participant2_SD_plasma_24hrs


title: 4634_OM2A_7-4-G11-M7


title: 4634_OM2A_7-4-G11-N19


title: 4634_OM2A_7-4-G11-M8


title: 4634_OM2A_7-4-G11-M20


title: 4634_OM2A_7-4-G11-O20


title: Participant1_SD_plasma_0hr


title: 4634_OM2A_7-4-G11-M19


title: Participant4_CTRL_plasma_24hrs


title: Participant5_CTRL_plasma_24hrs


title: 4634_OM2A_7-4-G11-L7


title: 4634_OM2A_7-4-G11-O19


title: Participant2_CTRL_plasma_24hrs


title: 4634_OM2A_7-4-G11-L19


title: 4634_OM2A_7-4-G11-L20


title: Participant1_CTRL_plasma_24hrs


title: Participant3_CTRL_plasma_24hrs


title: 4634_OM2A_7-4-G11-K8


title: Participant4_SD_plasma_0hr


title: 4634_OM2A_7-4-G11-K20


title: 4634_OM2A_7-4-G11-K19


title: 4634_OM2A_7-4-G11-K7


title: 4634_OM2A_7-4-G11-J8


title: Participant3_CTRL_plasma_0hr


title: Participant5_CTRL_plasma_0hr


title: Participant4_CTRL_plasma_0hr


title: 4634_OM2A_7-4-G11-J7


title: 4634_OM2A_7-4-G11-I8


title: 4634_OM2A_7-4-G11-J19


title: SM-LNJMX_S471_E1-50

city: Boston

first: Yu

last: Zhang

state: MA

address: 3 Blackfan Street, Room 13060

department: Neurobiology

institute: Boston Childrens Hospital

description: SmartSeq

library_source: transcriptomic single cell

data_processing:
For 10x samples, sequencing reads were aligned to the mouse reference transcriptome (mm10, version 2020-A) using CellRanger (version 6.1.2). For SSv4, samples were aligned to Mouse reference, mm10/genecode.vM23, using STAR (version 2.7.1a).
Assembly: mouse reference transcriptome (mm10)
Supplementary files format and content: .rda files. R object with count matrix

molecule: nuclear RNA

organism: Mus musculus

extract_protocol:
Single nuclei were isolated by adapting a protocol from the Allen Institue for Brain Science available at protocols.io (dx.doi.org/10.17504/protocols.io.bq7emzje). Spinal projecting neurons (SPNs) were enriched from the nucleus suspensions using fluorescence activated nucleus sorting (FANS). For 10x, single SPN nuclei were sorted using a BD FACSARIA II with a 70 µm custom pressure nozzle (50 psi). Briefly, single nuclei were captured by sorting on ‘four-way-purity mode’ and gating on DAPI-positive while excluding debris and aggregates, then gating on GFP and/or mScarlet signal. Nuclei were sorted (to a maximum number of 16,000 nuclei) using two-way sorting (GFP-positive separate from mScarlet- and double-positive) into PCR tubes (pre-coated with 5% BSA overnight) containing 20uL of sort buffer. After sorting, PCR tubes were briefly centrifuged and then placed on ice until proceeding with the 10x platform. For SSv4, single nuclei were sorted using a Sony SH800 Cell Sorter or MA900 Multi-Application Cell Sorter using a 100 µm chip. Briefly, single nuclei were sorted on ‘single-cell’ mode and captured by gating on singlet DAPI-positive, then gating on GFP and/or mScarlet signal to specifically sort cervical and/or lumbar project SPNs, respectively. Indexed plate-based sorting was used to sort GFP-, mScarlet, and double-positive nuclei into strip tubes containing 11.5uL of SMART-Seq v4 lysis buffer. Lysed FANS-sorted nuclei were then briefly centrifuged, frozen on dry ice, and stored at -80ºC until proceeding with the SSv4 platform.
10x processing was performed using the Chromium Next GEM Single Cell 3ʹ Kit v3.1 (1000268, 10x Genomics). To optimize yield, sorted nucleus suspensions were loaded directly into the 10x Chromium Controller without post-FANS spin down and re-suspension. Reverse transcription, cDNA amplification, and library construction were performed according to the manufacturer’s protocol. Libraries were sequenced on an Illumina NovaSeq 6000, targeted at a sequencing depth of 120,000 reads per nucleus. SMART-Seq v4 (SSv4) processing was performed according to previously established procedures47 available at protocols.io (dx.doi.org/10.17504/protocols.io.8epv517xdl1b/v2). Briefly, the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (634894, Takara) was used to reverse transcribe poly(A) RNA and amplify full-length cDNA. Samples were amplified for 16-21 cycles in eight-well strips. Library preparation was then performed using Nextera XT DNA Library Preparation (FC-131-1096, Illumina) with a custom index set (Integrated DNA Technologies) according to the manufacturer’s instructions with modifications to reduce the volumes of all reagents and cDNA input to 0.2x of the original protocol. Libraries were sequenced on either an Illumina NovaSeqSP-XP or an Illumina NextSeq2000, targeting 500,000 reads per nucleus.
Single-nucleus RNA sequencing (snRNAseq)

value: Spinal Projecting Neuron (SPN)

tag: strain

value: 57 days


title: 4634_OM2A_7-4-G11-I7


title: SM-LNJMX_S472_E1-50

description: SmartSeq

tag: cell type


title: SM-LNJMX_S468_E1-50

description: SmartSeq


title: SM-LNJMX_S470_E1-50

description: SmartSeq


title: SM-LNJMX_S469_E1-50

description: SmartSeq


title: Participant1_CTRL_plasma_0hr


title: 4634_OM2A_7-4-G11-I20


title: 4634_OM2A_7-4-G11-I19


title: SM-LNJMX_S467_E1-50

description: SmartSeq


title: 4634_OM2A_7-4-G11-H8


title: SM-LNJMX_S466_E1-50

description: SmartSeq


title: 4634_OM2A_7-4-G11-H7


title: SM-LNJMX_S465_E1-50

description: SmartSeq


title: SM-LNJMX_S464_E1-50

description: SmartSeq


title: 4634_OM2A_7-4-G11-H20


title: 4634_OM2A_7-4-G11-J20


title: 4634_OM2A_7-4-G11-H19


title: 4634_OM2A_7-4-G11-G8


title: SM-LNJMX_S461_E1-50

description: SmartSeq


title: 4634_OM2A_7-4-G11-G20


title: Participant2_CTRL_plasma_0hr


title: SM-LNJMX_S458_E1-50

description: SmartSeq


title: SM-LNJMX_S462_E1-50

description: SmartSeq


title: SM-LNJMX_S460_E1-50

description: SmartSeq


title: 4634_OM2A_7-4-G11-N20


title: 4634_OM2A_7-4-G11-G19


title: 4634_OM2A_7-4-G11-F8


title: 4634_OM2A_7-4-G11-F7


title: SM-LNJMX_S456_E1-50

description: SmartSeq


title: 4634_OM2A_7-4-G11-F20


title: SM-LNJMX_S455_E1-50

description: SmartSeq


title: SM-LNJMX_S454_E1-50

description: SmartSeq


title: SM-LNJMX_S453_E1-50

description: SmartSeq


title: SM-LNJMX_S452_E1-50

description: SmartSeq


title: SM-LNJMX_S463_E1-50

description: SmartSeq


title: 4634_OM2A_7-4-G11-E7


title: 4634_OM2A_7-4-G11-E20


title: SM-LNJMX_S459_E1-50

description: SmartSeq


title: 4634_OM2A_7-4-G11-G7


title: 4634_OM2A_7-4-G11-D8


title: SM-LNJMX_S450_E1-50

description: SmartSeq


title: 4634_OM2A_7-4-G11-D7


title: 4634_OM2A_7-4-G11-E19


title: SM-LNJMX_S457_E1-50

description: SmartSeq


title: SM-LNJMX_S449_E1-50

description: SmartSeq


title: 4634_OM2A_7-4-G11-D20


title: SM-LNJMX_S451_E1-50

description: SmartSeq


title: SM-LNJMX_S446_E1-50

description: SmartSeq


title: SM-LNJMX_S447_E1-50

description: SmartSeq


title: SM-LNJMX_S448_E1-50

description: SmartSeq


title: 4634_OM2A_7-4-G11-F19


title: 4634_OM2A_7-4-G11-D19


title: 4634_OM2A_7-4-G11-C8


title: SM-LNJMX_S444_E1-50

description: SmartSeq


title: 4634_OM2A_7-4-G11-E8


title: 4634_OM2A_7-4-G11-C7


title: SM-LNJMX_S443_E1-50

description: SmartSeq


title: 4634_OM2A_7-4-G11-C19


title: 4634_OM2A_7-4-G11-B8


title: SM-LNJMX_S440_E1-50

description: SmartSeq

value: 56 days


title: SM-LNJMX_S445_E1-50

description: SmartSeq


title: 4634_OM2A_7-4-G11-C20


title: 4634_OM2A_7-4-G11-B20


title: SM-LNJMX_S438_E1-50

description: SmartSeq


title: SM-LNJMX_S441_E1-50

description: SmartSeq


title: SM-LNJMX_S439_E1-50

description: SmartSeq


title: SM-LNJMX_S436_E1-50

description: SmartSeq


title: SM-LNJMX_S437_E1-50

description: SmartSeq


title: 4634_OM2A_7-4-G11-A7


title: 4634_OM2A_7-4-G11-B19


title: SM-LNJMX_S435_E1-50

description: SmartSeq


title: 4634_OM2A_7-4-G11-A8


title: SM-LNJMX_S434_E1-50

description: SmartSeq


title: 4634_OM2A_7-4-G11-A19


title: SM-LNJMX_S442_E1-50

description: SmartSeq


title: SM-LNJMX_S432_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-P18


title: 4634_OM2A_7-3-G11-P5


title: SM-LNJMX_S433_E1-50

description: SmartSeq


title: SM-LNJMX_S431_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-P6


title: SM-LNJMX_S430_E1-50

description: SmartSeq


title: 4634_OM2A_7-4-G11-B7


title: 4634_OM2A_7-3-G11-O6


title: 4634_OM2A_7-3-G11-P17


title: SM-LNJMX_S428_E1-50

description: SmartSeq


title: SM-LNJMX_S429_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-O5


title: SM-LNJMX_S427_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-O18


title: SM-LNJMX_S426_E1-50

description: SmartSeq


title: 4634_OM2A_7-4-G11-A20


title: 4634_OM2A_7-3-G11-O17


title: 4634_OM2A_7-3-G11-N6


title: SM-LNJMX_S381_E1-50

description: SmartSeq


title: SM-LNJMX_S383_E1-50

description: SmartSeq


title: SM-LNJMX_S384_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-N17


title: SM-LNJMX_S382_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-M6


title: SM-LNJMX_S380_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-M5


title: SM-LNJMX_S379_E1-50

description: SmartSeq


title: SM-LNJMX_S378_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-M18


title: SM-LNJMX_S377_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-L6


title: SM-LNJMX_S368_E1-50

description: SmartSeq


title: SM-LNJMX_S367_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-L17


title: 4634_OM2A_7-3-G11-L5


title: SM-LNJMX_S366_E1-50

description: SmartSeq


title: SM-LNJMX_S365_E1-50

description: SmartSeq


title: SM-LNJMX_S425_E1-50

description: SmartSeq


title: SM-LNJMX_S364_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-N5


title: 4634_OM2A_7-3-G11-K5


title: 4634_OM2A_7-3-G11-N18


title: SM-LNJMX_S363_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-K18


title: 4634_OM2A_7-3-G11-J6


title: SM-LNJMX_S360_E1-50

description: SmartSeq


title: SM-LNJMX_S361_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-K17


title: SM-LNJMX_S359_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-J5


title: 4634_OM2A_7-3-G11-J18


title: SM-LNJMX_S358_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-M17


title: 4634_OM2A_7-3-G11-J17


title: SM-LNJMX_S356_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-I5


title: 4634_OM2A_7-3-G11-L18


title: SM-LNJMX_S355_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-I18


title: SM-LNJMX_S354_E1-50

description: SmartSeq


title: SM-LNJMX_S353_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-K6


title: 4634_OM2A_7-3-G11-I17


title: 4634_OM2A_7-3-G11-H6


title: SM-LNJMX_S352_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-H5


title: SM-LNJMX_S351_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-H18


title: SM-LNJMX_S362_E1-50

description: SmartSeq


title: SM-LNJMX_S350_E1-50

description: SmartSeq


title: SM-LNJMX_S349_E1-50

description: SmartSeq


title: SM-LNJMX_S348_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-G6


title: SM-LNJMX_S347_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-I6


title: 4634_OM2A_7-3-G11-G5


title: 4634_OM2A_7-3-G11-G18


title: 4634_OM2A_7-3-G11-G17


title: SM-LNJMX_S357_E1-50

description: SmartSeq


title: SM-LNJMX_S346_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-F6


title: 4634_OM2A_7-3-G11-F5


title: SM-LNJMX_S344_E1-50

description: SmartSeq


title: SM-LNJMX_S345_E1-50

description: SmartSeq


title: SM-LNJMX_S343_E1-50

description: SmartSeq


title: SM-LNJMX_S342_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-F18


title: SM-LNJMX_S341_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-F17


title: SM-LNJMX_S340_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-E6


title: SM-LNJMX_S339_E1-50

description: SmartSeq


title: SM-LNJMX_S338_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-E18


title: 4634_OM2A_7-3-G11-E17


title: 4634_OM2A_7-3-G11-E5


title: SM-LNJMX_S337_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-D6


title: SM-LNJMX_S336_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-D5


title: 4634_OM2A_7-3-G11-H17


title: SM-LNJMX_S335_E1-50

description: SmartSeq


title: SM-LNJMX_S334_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-D18


title: 4634_OM2A_7-3-G11-C6


title: 4634_OM2A_7-3-G11-C5


title: SM-LNJMX_S332_E1-50

description: SmartSeq


title: SM-LNJMX_S333_E1-50

description: SmartSeq


title: SM-LNJMX_S331_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-D17


title: 4634_OM2A_7-3-G11-C18


title: 4634_OM2A_7-3-G11-B5


title: 4634_OM2A_7-3-G11-B6


title: SM-LNJMX_S329_E1-50

description: SmartSeq


title: SM-LNJMX_S328_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-C17


title: 4634_OM2A_7-3-G11-B18


title: SM-LNJMX_S326_E1-50

description: SmartSeq


title: SM-LNJMX_S327_E1-50

description: SmartSeq


title: SM-LNJMX_S330_E1-50

description: SmartSeq


title: SM-LNJMX_S324_E1-50

description: SmartSeq


title: SM-LNJMX_S325_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-A6


title: 4634_OM2A_7-3-G11-B17


title: 4634_OM2A_7-3-G11-A18


title: 4634_OM2A_7-3-G11-A5


title: SM-LNJMX_S323_E1-50

description: SmartSeq


title: 4634_OM2A_7-3-G11-A17


title: SM-LNJMX_S322_E1-50

description: SmartSeq


title: SM-LNJMX_S321_E1-50

description: SmartSeq


title: SM-LNJMX_S320_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-P3


title: 4634_OM2A_7-2-G11-P16


title: SM-LNJMX_S318_E1-50

description: SmartSeq


title: SM-LNJMX_S317_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-O4


title: SM-LNJMX_S316_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-O16


title: SM-LNJMX_S315_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-O15


title: SM-LNJMX_S314_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-N4


title: SM-LNJMX_S312_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-N3


title: SM-LNJMX_S311_E1-50

description: SmartSeq


title: SM-LNJMX_S310_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-N16


title: 4634_OM2A_7-2-G11-P4


title: SM-LNJMX_S309_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-N15


title: SM-LNJMX_S308_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-M4


title: SM-LNJMX_S319_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-M3


title: SM-LNJMX_S306_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-M16


title: 4634_OM2A_7-2-G11-P15


title: 4634_OM2A_7-2-G11-M15


title: SM-LNJMX_S305_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-L4


title: SM-LNJMX_S304_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-L3


title: SM-LNJMX_S313_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-O3


title: SM-LNJMX_S303_E1-50

description: SmartSeq


title: SM-LNJMX_S302_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-L15


title: 4634_OM2A_7-2-G11-L16


title: 4634_OM2A_7-2-G11-K4


title: SM-LNJMX_S300_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-K3


title: 4634_OM2A_7-2-G11-K16


title: SM-LNJMX_S298_E1-50

description: SmartSeq


title: SM-LNJMX_S299_E1-50

description: SmartSeq


title: SM-LNJMX_S307_E1-50

description: SmartSeq


title: SM-LNJMX_S297_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-J4


title: 4634_OM2A_7-2-G11-J3


title: SM-LNJMX_S296_E1-50

description: SmartSeq


title: SM-LNJMX_S295_E1-50

description: SmartSeq


title: SM-LNJMX_S294_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-K15


title: 4634_OM2A_7-2-G11-J16


title: SM-LNJMX_S293_E1-50

description: SmartSeq


title: SM-LNJMX_S291_E1-50

description: SmartSeq


title: SM-LNJMX_S292_E1-50

description: SmartSeq


title: SM-LNJMX_S290_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-J15


title: 4634_OM2A_7-2-G11-I16


title: 4634_OM2A_7-2-G11-I4


title: 4634_OM2A_7-2-G11-I3


title: SM-LNJMX_S301_E1-50

description: SmartSeq


title: SM-LNJMX_S289_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-I15


title: SM-LNJMX_S288_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-H4


title: 4634_OM2A_7-2-G11-H3


title: SM-LNJMX_S287_E1-50

description: SmartSeq


title: SM-LNJMX_S286_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-H15


title: SM-LNJMX_S285_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-G4


title: SM-LNJMX_S284_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-G3


title: 4634_OM2A_7-2-G11-G16


title: SM-LNJMX_S283_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-G15


title: SM-LNJMX_S282_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-F4


title: SM-LNJMX_S280_E1-50

description: SmartSeq


title: SM-LNJMX_S281_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-F3


title: SM-LNJMX_S279_E1-50

description: SmartSeq


title: SM-LNJMX_S278_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-F16


title: 4634_OM2A_7-2-G11-E4


title: SM-LNJMX_S276_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-E3


title: 4634_OM2A_7-2-G11-F15


title: SM-LNJMX_S277_E1-50

description: SmartSeq


title: SM-LNJMX_S274_E1-50

description: SmartSeq


title: SM-LNJMX_S275_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-E15


title: 4634_OM2A_7-2-G11-E16


title: 4634_OM2A_7-2-G11-H16


title: SM-LNJMX_S273_E1-50

description: SmartSeq


title: SM-LNJMX_S264_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-D4


title: SM-LNJMX_S263_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-D16


title: 4634_OM2A_7-2-G11-D3


title: 4634_OM2A_7-2-G11-D15


title: SM-LNJMX_S262_E1-50

description: SmartSeq


title: SM-LNJMX_S261_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-C4


title: SM-LNJMX_S260_E1-50

description: SmartSeq


title: SM-LNJMX_S259_E1-50

description: SmartSeq


title: SM-LNJMX_S258_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-C3


title: 4634_OM2A_7-2-G11-C15


title: 4634_OM2A_7-2-G11-B4


title: SM-LNJMX_S256_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-B15


title: 4634_OM2A_7-2-G11-B3


title: 4634_OM2A_7-2-G11-B16


title: SM-LNJMX_S255_E1-50

description: SmartSeq


title: SM-LNJMX_S254_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-A4


title: SM-LNJMX_S253_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-A16


title: 4634_OM2A_7-2-G11-A15


title: SM-LNJMX_S250_E1-50

description: SmartSeq


title: SM-LNJMX_S251_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-A3


title: 4634_OM2A_7-1-G11-P2


title: SM-LNJMX_S249_E1-50

description: SmartSeq


title: 4634_OM2A_7-2-G11-C16


title: SM-LNJMX_S247_E1-50

description: SmartSeq


title: SM-LNJMX_S248_E1-50

description: SmartSeq


title: 4634_OM2A_7-1-G11-P13


title: 4634_OM2A_7-1-G11-P14


title: 4634_OM2A_7-1-G11-O1


title: SM-LNJMX_S244_E1-50

description: SmartSeq


title: SM-LNJMX_S257_E1-50

description: SmartSeq


title: SM-LNJMX_S245_E1-50

description: SmartSeq