title: si-Ctrl_rep1

city: Irvine

first: Christopher

middle: J

last: Hatch

address: 2400 Biological Sciences III

institute: University of California, Irvine

description: AGTGTACT-AAGTATGC, AGTGTACT-AAGTATGC

data_processing:
Transcriptomic sequencing of siRNA-transfected EC was performed using an Illumina NovaSeq 6000 through the University of California-Irvine Genomics, Research and Technology Hub. FASTQ files underwent trimming of adapter regions and removal of low-quality reads with fastp. GRCh38 reference genome was built using bowtie2 from Ensembl release 105 and FASTQ files were aligned to reference genome using STAR. FASTQ files were merged using SAMtools and a count matrix of exon protein coding genes was generated using featureCounts. The count matrix was imported into RStudio running R [version 4.0.3] and differentially expressed genes for each condition were determined using DESeq2.
Assembly: GRCh38
Supplementary files format and content: Tab delimited count matrix

source_name: Human umbilical vein endothelial cells

growth_protocol: EC were cultured on gelatinized surfaces in complete EGM2 (Lonza).

extract_protocol:
Cells were lifted from culture flasks with 0.05% trypsin, pelleted, and resuspended in EGM-2 (Lonza)
Poly-A enrichment library construction

value: Human umbilical vein endothelial cells

value: silencing RNA scrambled control


title: TIGIThigh replicate3

status: Public on Dec 14 2023

type: SRA

city: West Lafayette

first: Sagar

middle: M

last: Utturkar

state: IN

address: 201 S University St

department: Institute for Cancer Research

country: USA

institute: Purdue University

library_source: transcriptomic

data_processing:
Data quality control (adapter trimming, minimum Phred score 30, minimum read length 50 bp) was performed using fastp 27 (version 0.19.5).
Quality trimmed reads were mapped to human reference genome (GRCh38) using the STAR aligner 28 (version 2.7.9a).
Reads aligned to each gene feature were counted using the featureCounts 29 program from Subread package (version 1.6.1).
Differential expression (DE) analysis was performed using the edgeR method 30 (version 3.32.1).
Assembly: GRCh38
Supplementary files format and content: Excel file containing raw counts for each sample

molecule: total RNA

organism: Homo sapiens

source_name: human NK cells from peripheral blood

extract_protocol:
mirVana miRNA Isolation Kit
Samples were analyzed by Agilent Bioanalyzer, with DV200 > 80 and RIN > 8.4 for all samples. Libraries were prepared by GeneWiz. Sample libraries contained at least 30 million paired-end reads with length of 250 bp.

tag: cell type

value: human NK cells from peripheral blood


title: CT27 mCherry high A 24 HPI

city: Minneapolis

first: Craig

middle: John

last: Bierle

state: Minnesota

address: 2001 6th Street SE

department: Pediatrics

institute: University of Minnesota

description:
27_HAH_24
Bierle_Project_013_raw_Gencode_v38_counts.csv
Bierle_Project_013_raw_EF999921.1_counts.csv

data_processing:
Sequencing reads were trimmed to remove adapter sequences with Trimmomatic using default parameters.
Trimmed reads were aligned to a concatenated reference file that contained the human (hg38) and TB40e-BAC4 (NCBI EF999921.1) genomes with STAR version 2.7.2a using the default parameters.
Gene count tables were prepared with featureCounts.
Assembly: hg38 + TB40e-Bac4 [NCBI EF999921.1]
Supplementary files format and content: text file includes raw counts for each sample mapped to hg38
Supplementary files format and content: text file includes raw counts for each sample mapped to HCMV TB40e-Bac4 (NCBI EF999921.1)

growth_protocol: CT27 (RCB4936) and CT29 (RCB4937) cells were  maintained using "TS culture protocol_V2" (doi: 10.1073/pnas.1916019116)  and differentiated as previously described (doi: 10.1016/j.stem.2017.11.004).

extract_protocol:
Total RNA was extracted using a RNeasy Mini kit (Qiagen).
SMARTer Pico Mammalian V2 kit (Takara)
TruSeq Stranded mRNA (Illumina)

treatment_protocol: Cells were either mock or HCMV (TB40/Ewt-mCherry) infected at a MOI of 1.

tag: cell line

value: Trophoblast Stem Cells

tag: genotype

value: WT

tag: treatment

value: Infected 24 HPI mCherry high


title: si-Ctrl_rep2

state: CA

description: TATACCAT-GTGGCATT, TATACCAT-GTGGCATT


title: TIGIThigh replicate2


title: HCT116_CTRL_clone_29_repl_B

city: Milano

first: Viviana

last: Piccolo

state: Milano

address: Via Adamello 16

department: Department of Experimental Oncology

country: Italy

institute: European Institute of Oncology (IEO)

description: 4sU-RNAseq CTRL cl 29 repl B

data_processing:
After quality filtering according to the Illumina pipeline and after checking reads quality using FastQC v0.11.2 (http://www.bioinformatics.babraham.ac.uk/projects/fastqc/), strand specific paired end reads (51 bp) were aligned using TopHat v2.1.0 1 (PMID:23618408), allowing up to two mismatches and using the option --b2-very- sensitive --library-type fr-firststrand. Indels due to sequencing errors were identified using Bowtie2 v 2.2.6 (PMID: 19261174). Reads originating from the two strands were separated according to the XS:A:+/XS:A:-flag provided by TopHat2 (PMID:23618408).
SICER v2 (PMID: 24743992) was used to detect the extra-genic transcripts regulated in cells depleted of individual factors. The entire genome was partitioned into blocks of non-overlapping 500 bp windows with a gap size <1000 nt. An effective genome fraction of 1, a fragment size of 0 and a False Discovery Rate (FDR) cut-off <0.01 were used.
Tracks (*.bigWig files) were generated using bamCoverage from deepTools v.3.5 (PMID:27079975) (-bs 1 --normalizeUsing RPKM --outFileFormat bigwig -filterRNAstrand forward or --filterRNAstrand reverse)
Assembly: hg38 (GENCODE) https://www.gencodegenes.org/human/release_33.html
Supplementary files format and content: Strand specific tracks (bigWig)
Library strategy: 4sU-RNAseq

source_name: HCT-116, human colon, colorectal carcinoma

growth_protocol: ZC3H4-mAID:dTAG-PNUTS HCT-116 cells were cultured in McCoy’s 5A Medium Modified with 10% South American foetal calf serum,1% pen/strep and 1% L-Glutamax.

extract_protocol:
Total RNA was extracted using Trizol (Thermo-Fisher cat. 15596026); 4sU-labelled RNA was extracted as described (PMID 26593720)
Library preparation was performed using the TruSeq Stranded Total RNA sample preparation kit (Illumina RS-122-9007) using 80-200 ng of nascent 4sU-labelled RNA, without ribosomal depletion.

treatment_protocol: Auxin (100 uM) and (dTAG-13) 500 nM were added 24h before harvesting the cells.  4-thio-uridine (4sU) was added to the medium at a final concentration of 500 uM for 10 min before harvesting the cells.

value: epithelial cell

tag: time


title: HCT116_CTRL_clone_29_repl_A

description: 4sU-RNAseq CTRL cl 29 repl A

value: HCT-116, human colon, colorectal carcinoma


title: HCT116_AUX_dTAG PNUTS_clone_30_repl_B

description: 4sU-RNAseq AUX_dTAG cl 30 repl B

value: Auxin 100 uM_dTAG13 500 nM


title: HCT116_dTAG PNUTS_clone_29_repl_B

description: 4sU-RNAseq dTAG cl 29 repl B

value: dTAG13 500 nM


title: HCT116_dTAG PNUTS_clone_30_repl_B

description: 4sU-RNAseq dTAG cl 30 repl B


title: HCT116_dTAG PNUTS_clone_29_repl_A

description: 4sU-RNAseq dTAG cl 29 repl A


title: HCT116_AUX_clone_29_repl_B

description: 4sU-RNAseq AUX cl 29 repl B

value: Auxin 100 uM


title: HCT116_AUX_dTAG PNUTS_clone_29_repl_B

description: 4sU-RNAseq AUX_dTAG cl 29 repl B


title: TIGITlow replicate2


title: HCT116_AUX_dTAG PNUTS_clone_29_repl_A

description: 4sU-RNAseq AUX_dTAG cl 29 repl A


title: TIGITlow replicate1


title: HCT116_AUX_clone_29_repl_A

description: 4sU-RNAseq AUX cl 29 repl A


title: HCT116_AUX_dTAG PNUTS_clone_30_repl_A

description: 4sU-RNAseq AUX_dTAG cl 30 repl A


title: TIGIThigh replicate1


title: HCT116_AUX_clone_30_repl_B

description: 4sU-RNAseq AUX cl 30 repl B


title: HCT116_dTAG PNUTS_clone_1_repl_B

description: 4sU-RNAseq dTAG cl 1 repl B


title: HCT116_AUX_dTAG PNUTS_clone_1_repl_B

description: 4sU-RNAseq AUX_dTAG cl 1 repl B


title: HCT116_CTRL_clone_30_repl_B

description: 4sU-RNAseq CTRL cl 30 repl B


title: si-Cx40_rep2

description: AGTTCATA-TGAAGGCC, AGTTCATA-TGAAGGCC

value: silencing RNA Cx40


title: HCT116_CTRL_clone_1_repl_B

description: 4sU-RNAseq CTRL cl 1 repl B


title: si-Cx40_rep1

description: TCAATTAT-CCTTCAGG, TCAATTAT-CCTTCAGG


title: HCT116_dTAG PNUTS_clone_30_repl_A

description: 4sU-RNAseq dTAG cl 30 repl A


title: HCT116_AUX_clone_1_repl_A

description: 4sU-RNAseq AUX cl 1 repl A


title: HCT116_AUX_clone_30_repl_A

description: 4sU-RNAseq AUX cl 30 repl A


title: HCT116_AUX_dTAG PNUTS_clone_1_repl_A

description: 4sU-RNAseq AUX_dTAG cl 1 repl A


title: HCT116_CTRL_clone_1_repl_A

description: 4sU-RNAseq CTRL cl 1 repl A


title: HCT116_AUX_clone_1_repl_B

description: 4sU-RNAseq AUX cl 1 repl B


title: HCT116_AUX_dTAG SYMPK_rep_2

description: 4sU-RNAseq AUX_dTAG repl 2

library_source: other

data_processing:
After quality filtering according to the Illumina pipeline and after checking reads quality using FastQC v0.11.2 (http://www.bioinformatics.babraham.ac.uk/projects/fastqc/), strand specific paired end reads (51 bp) were aligned using TopHat v2.1.0 1 (PMID:23618408), allowing up to two mismatches and using the option --b2-very- sensitive --library-type fr-firststrand. Indels due to sequencing errors were identified using Bowtie2 v 2.2.6 (PMID: 19261174). Reads originating from the two strands were separated according to the XS:A:+/XS:A:-flag provided by TopHat2 (PMID:23618408).
SICER v2 (PMID: 24743992) was used to detect the extra-genic transcripts regulated in cells depleted of individual factors. The entire genome was partitioned into blocks of non-overlapping 500 bp windows with a gap size <1000 nt. An effective genome fraction of 1, a fragment size of 0 and a False Discovery Rate (FDR) cut-off <0.01 were used.
Tracks (*.bigWig files) were generated using bamCoverage from deepTools v.3.5 (PMID:27079975) (-bs 1 --normalizeUsing RPKM --outFileFormat bigwig -filterRNAstrand forward or --filterRNAstrand reverse)
Assembly: hg38 (GENCODE) https://www.gencodegenes.org/human/release_33.html
Supplementary files format and content: Strand specific tracks (bigWig)

molecule: other

growth_protocol: ZC3H4-mAID:dTAG-SYMPK HCT-116 cells were cultured in McCoy’s 5A Medium Modified with 10% South American foetal calf serum,1% pen/strep and 1% L-Glutamax.


title: HCT116_CTRL_clone_30_repl_A

description: 4sU-RNAseq CTRL cl 30 repl A


title: HCT116_dTAG PNUTS_clone_1_repl_A

description: 4sU-RNAseq dTAG cl 1 repl A


title: HCT116_dTAG SYMPK_rep_2

description: 4sU-RNAseq dTAG repl 2


title: HCT116_dTAG SYMPK_rep_1

description: 4sU-RNAseq dTAG repl 1


title: HCT116_siSYMPK_AUX_repl_2

description: 4sU-RNAseq siSYMPK_AUX repl 2

data_processing:
After quality filtering according to the Illumina pipeline and after checking reads quality using FastQC v0.11.2 (http://www.bioinformatics.babraham.ac.uk/projects/fastqc/), strand specific paired-end reads (51 bp) were aligned using TopHat v2.1.0 1 (PMID:23618408), allowing up to two mismatches and using the option --b2-very- sensitive --library-type fr-firststrand. Indels due to sequencing errors were identified using Bowtie2 v 2.2.6 (PMID: 19261174). Reads originating from the two strands were separated according to the XS:A:+/XS:A:-flag provided by TopHat2 (PMID:23618408).
SICER v2 (PMID: 24743992) was used to detect the extra-genic transcripts regulated in cells depleted of individual factors. The entire genome was partitioned into blocks of non-overlapping 500 bp windows with a gap size <1000 nt. An effective genome fraction of 1, a fragment size of 0
EdgeR v3.10.5 PMID: 19910308 was used for the differentaial analysis.The standard Counts Per Million (CPM) for all conditions were adjusted with the Trimmed Mean of M-values (TMM) using the calcNormFactors() function. We modeled the data variability estimating the dispersion of the negative binomial model using the quantile-adjusted Conditional Maximum Likelihood (qCML) method. We first used the (qCML) estimateCommonDisp() function and then the (qCML)  estimateTagwiseDisp() function, both from the edgeR Bioconductor package. We finally computed exact p-values for the negative binomial distribution using the function exactTest(), making a pairwise comparisons between the groups.
Tracks (*.bigWig files) were generated using bamCoverage from deepTools v.3.5 (PMID:27079975) (-bs 1 --normalizeUsing RPKM --outFileFormat bigwig -filterRNAstrand forward or --filterRNAstrand reverse)
Assembly: hg38 (GENCODE) https://www.gencodegenes.org/human/release_33.html
Supplementary files format and content: Strand specific tracks (bigWig)

growth_protocol: ZC3H4-mAID HCT-116 cells were cultured in McCoy’s 5A Medium Modified with 10% South American foetal calf serum serum,1% pen/strep and 1% L-Glutamax.

extract_protocol:
Total RNA was extracted using Trizol (Thermo Fisher cat. 15596026); 4sU-labelled RNA was extracted as described (PMID 26593720)
Library preparation was performed using the TruSeq Stranded Total RNA sample preparation kit (Illumina RS-122-9007) using 80-200 ng of nascent 4sU-labelled RNA, without ribosomal depletion.

treatment_protocol: Cells were transfected with non-targeting siRNA or SYMPK-targeting siRNA. Auxin (100) uM was added 4h before harvesting the cells.  4-thio-uridine (4sU) was added to the medium at a final concentration of 500 uM for 10 min before harvesting the cells.

value: siSYMPK_Auxin 100 uM


title: HCT116_CTRL rep_2

description: 4sU-RNAseq CTRL repl 2


title: HCT116_siSYMPK_repl_3

description: 4sU-RNAseq siSYMPK repl 3

value: siSYMPK


title: HCT116_AUX_dTAG SYMPK_rep_1

description: 4sU-RNAseq AUX_dTAG repl 1


title: HCT116_AUX_rep_2

description: 4sU-RNAseq AUX repl 2


title: HCT116_CTRL_rep_1

description: 4sU-RNAseq CTRL repl 1


title: HCT116_AUX_rep_1

description: 4sU-RNAseq AUX repl 1


title: HCT116_siSYMPK_AUX_repl_3

description: 4sU-RNAseq siSYMPK_AUX repl 3


title: HCT116_siSYMPK_AUX_repl_1

description: 4sU-RNAseq siSYMPK_AUX repl 1


title: HCT116_CTRL_repl_1

description: 4sU-RNAseq siCTRL repl 1

value: siCTRL


title: HCT116_CTRL_AUX_repl_3

description: 4sU-RNAseq siCTRL_AUX repl 3

value: siCTRL_Auxin 100 uM


title: HCT116_siSYMPK_repl_2

description: 4sU-RNAseq siSYMPK repl 2


title: HCT116_siSYMPK_repl_1

description: 4sU-RNAseq siSYMPK repl 1


title: HCT116_CTRL_repl_3

description: 4sU-RNAseq siCTRL repl 3


title: HCT116_CTRL_repl_2

description: 4sU-RNAseq siCTRL repl 2


title: HeLa-TREx_ZC3H4_CTERMINUS clone 3  (REP1)

description: 4sU-RNAseq C-TERMINUS clone 3

data_processing:
After quality filtering according to the Illumina pipeline and after checking reads quality using FastQC v0.11.2 (http://www.bioinformatics.babraham.ac.uk/projects/fastqc/), strand specific paired-end reads (51 bp) were aligned using TopHat v2.1.0 1 (PMID:23618408), allowing up to two mismatches and using the option --b2-very- sensitive --library-type fr-firststrand. Indels due to sequencing errors were identified using Bowtie2 v 2.2.6 (PMID: 19261174). Reads originating from the two strands were separated according to the XS:A:+/XS:A:-flag provided by TopHat2 (PMID:23618408).
SICER v2 (PMID: 24743992) was used to detect the extra-genic transcripts regulated in cells depleted of individual factors. The entire genome was partitioned into blocks of non-overlapping 500 bp windows with a gap size <1000 nt. An effective genome fraction of 1, a fragment size of 0 and a False Discovery Rate (FDR) cut-off <0.01 were used.
Tracks (*.bigWig files) were generated using bamCoverage from deepTools v.3.5 (PMID:27079975) (-bs 1 --normalizeUsing RPKM --outFileFormat bigwig -filterRNAstrand forward or --filterRNAstrand reverse)
Assembly: hg38 (GENCODE) https://www.gencodegenes.org/human/release_33.html
Supplementary files format and content: Strand specific tracks (bigWig)

source_name: HeLa TREx Flp-In, human cervix, epitheloid carcinoma

growth_protocol: HeLa TREx Flp-In cells were cultured in DMEM containing 10% Tetracyclin-free Fetal Bovine Serum, 1% Penicillin/Streptomycin solution, 250 ug/mL Hygromicin B, 1ug/mL Blasticidin.

extract_protocol:
Total RNA was extracted using Trizol (Thermofisher cat. 15596026); 4sU-labelled RNA was extracted as described (Austenaa et al. 2015, PMID 26593720)
Library preparation was performed using the TruSeq Stranded Total RNA sample preparation kit (Illumina RS-122-9007) using 80-200 ng of 4sU-labelled RNA, without ribosomal depletion.

treatment_protocol: Cells were induced with 100 ng/ml tetracycline for 48 h to express the gene of interest. 4-thio-uridine (4sU) was added to the medium at a final concentration of 300 uM for 45 min before harvesting the cells.

value: Tetracycline 100 ng/ml


title: HCT116_CTRL_AUX_repl_2

description: 4sU-RNAseq siCTRL_AUX repl 2


title: HeLa-TREx_ZC3H4_FULL_LENGTH clone 9 (REP3)

description: 4sU-RNAseq Fulle length ZC3H4 clone 9

value: HeLa TREx Flp-In, human cervix, epitheloid carcinoma


title: HeLa-TREx_ZC3H4_FULL_LENGTH clone 4  (REP1)

description: 4sU-RNAseq Fulle length ZC3H4 clone 4


title: HeLa-TREx_EMPTY_VECTOR clone 6.1 (REP1)

description: 4sU-RNAseq empty vector clone 6


title: HCT116_CTRL_AUX_repl_1

description: 4sU-RNAseq siCTRL_AUX repl 1


title: HeLa-TREx_NTERMINUS clone 3 (REP1)

description: 4sU-RNAseq N-TERMINUS clone 3


title: HeLa-TREx_ZC3H4_CTERMINUS clone 5 (REP2)

description: 4sU-RNAseq C-TERMINUS clone 5


title: HeLa-TREx_ZC3H4_FULL_LENGTH clone 7 (REP2)

description: 4sU-RNAseq Fulle length ZC3H4 clone 7


title: HeLa-TREx_EMPTY_VECTOR clone 10.1 (REP2)

description: 4sU-RNAseq empty vector clone 10


title: HeLa-TREx_EMPTY_VECTOR clone 12.1 (REP3)

description: 4sU-RNAseq empty vector clone 12


title: HeLa-TREx_NTERMINUS clone 15 (REP3)

description: 4sU-RNAseq N-TERMINUS clone 15


title: HeLa-TREx_NTERMINUS clone 5 (REP2)

description: 4sU-RNAseq N-TERMINUS clone 5


title: HeLa-TREx_EMPTY_VECTOR clone 6.2 (REP1)

description: 4sU-RNAseq empty vector clone 6


title: vag bacteria supernatant NYC control r2

city: New York

first: Michal

middle: A

last: Elovitz

state: NY

address: 1468 Madison Ave

department: Obstetrics, Gynecology and Reproductive Sciences

institute: Icahn Mount Sinai

data_processing:
Illumina pipeline for basecalling and fastq demultiplexing
fastq data was counted against Gencode v38 with salmon
Assembly: GRCh38
Assembly: Gencode v38 transcripts
Supplementary files format and content: Salmon-calculated raw counts against Gencode v38 – gzipped, comma separated values

source_name: vaginal, VK2/E6E7

growth_protocol: Ectocervical, Endocervical and Vaginal cells were  were maintained in Kerotinocyte Serum Free Media (KSFM) supplemented with 0.1 ng/mL epidermal growth factor and 50 ug/mL bovine pituitary extract and antibiotics in humidified atmosphere with 5% CO2 at 37C

extract_protocol:
RNA was extracted from cells in trizol using the Qiagen-RNeasy Plus Mini kit following the manufacture's protocol
Illumina sequencing libraries were prepared using the Illumina TruSeq mRNA stranded library prep kit using the manufacture's protocol

treatment_protocol: Ectocervical, Endocervical and Vaginal cells were exposed to live bacteria or bacterial supernatants for 24 hours.

value: vaginal, VK2/E6E7

value: Bacteria Supernatants

tag: bacterial strain

value: NYC bacteria media


title: vag bacteria supernatant NYC control r3


title: HeLa-TREx_EMPTY_VECTOR clone 12.2 (REP3)

description: 4sU-RNAseq empty vector clone 12


title: vag live bacteria non-treated control r2


title: vag bacteria supernatant non-treated control r1


title: vag live bacteria non-treated control r3


title: vag LC bacteria supernatant r3

value: Lactobacillus crispatus


title: HeLa-TREx_EMPTY_VECTOR clone 10.2 (REP2)

description: 4sU-RNAseq empty vector clone 10


title: vag bacteria supernatant NYC control r1


title: vag bacteria supernatant non-treated control r2


title: HeLa-TREx_ZC3H4_CTERMINUS clone 9 (REP3)

description: 4sU-RNAseq C-TERMINUS clone 9


title: vag live bacteria non-treated control r1


title: vag LC bacteria supernatant r1


title: vag LC live bacteria r3

value: Live Bacteria


title: vag GV bacteria supernatant r1

value: Gardnerella vaginalis


title: vag GV live bacteria r2


title: vag LC live bacteria r2


title: vag GV live bacteria r1


title: vag GV bacteria supernatant r3


title: endo bacteria supernatant NYC control r1

source_name: endocervical, End1/E6E7

value: endocervical, End1/E6E7


title: vag GV live bacteria r3


title: vag LC live bacteria r1


title: endo bacteria supernatant non-treated control r1


title: endo bacteria supernatant NYC control r2


title: vag bacteria supernatant non-treated control r3


title: vag GV bacteria supernatant r2


title: endo bacteria supernatant NYC control r3


title: eno live bacteria non-treated control r3


title: vag LC bacteria supernatant r2


title: eno live bacteria non-treated control r2


title: eno live bacteria non-treated control r1


title: endo LC live bacteria r2


title: endo LC bacteria supernatant r2


title: endo GV bacteria supernatant r3


title: endo GV bacteria supernatant r2


title: endo LC bacteria supernatant r3


title: endo LC bacteria supernatant r1


title: endo GV bacteria supernatant r1


title: endo GV live bacteria r3


title: ecto bacteria supernatant NYC control r1

source_name: ectocervical, Ect1/E6E7

value: ectocervical, Ect1/E6E7


title: ecto bacteria supernatant non-treated control r3


title: endo GV live bacteria r2


title: endo bacteria supernatant non-treated control r3


title: ecto bacteria supernatant non-treated control r2


title: endo GV live bacteria r1


title: ecto bacteria supernatant non-treated control r1


title: ecto bacteria supernatant NYC control r3


title: ecto LC bacteria supernatants r1


title: endo bacteria supernatant non-treated control r2


title: ecto LC live bacteria r2


title: endo LC live bacteria r1


title: endo LC live bacteria r3


title: ecto live bacteria non-treated control r2


title: ecto live bacteria non-treated control r3


title: ecto LC bacteria supernatants r3


title: ecto bacteria supernatant NYC control r2


title: ecto LC bacteria supernatants r2


title: ecto LC live bacteria r3


title: ecto GV live bacteria r3


title: ecto GV bacteria supernatants r1


title: ecto LC live bacteria r1


title: ecto GV live bacteria r2


title: CD4+ T cell gene expression from Participant P (chip1, lane8) during acute HIV-1 infection

city: Silver Spring

first: Rasmi

last: Thomas

state: MD

address: 503 Robert Grant Avenue

department: MHRP

institute: Walter Reed Army Institute of Research

library_source: transcriptomic single cell

data_processing:
Sequencing reads were generated from bcl files using cellranger mkfastq
Reads were aligned to the Homo sapiens (GRCh38; Ensemble annotation 98) concatenated with HIV-1 genome (GenBank accession AB070352.1) using cellranger count
Gene expression per cell barcode was quantified using cellranger count
Assembly: GRCh38
Supplementary files format and content: gzipped barcode/features/matrix files include raw counts for each library

source_name: CD4+ T cell

extract_protocol:
Sorted CD4+ T cells were washed and resuspended in PBS. 14-21k cells were loaded into each well of 8 wells of a Chromium Next GEM Chip G chip and processed for scRNA-seq through the Chromium Controller (both 10x Genomics) instrument work-up per manufacturer's instructions
Library constructions were processed using the Chromium NextGEM Single Cell 5’ v1.1 Kit (10x Genomics) per manufacturer’s instructions

tag: disease state

value: acute HIV-1 infection

tag: tissue

value: CD4+ T cell

tag: 10x chip

tag: 10x lane


title: ecto GV bacteria supernatants r3


title: CD4+ T cell gene expression from Participant P (chip1, lane7) during acute HIV-1 infection


title: CD4+ T cell gene expression from Participant P (chip1, lane6) during acute HIV-1 infection


title: ecto GV bacteria supernatants r2


title: ecto GV live bacteria r1


title: CD4+ T cell gene expression from Participant P (chip1, lane4) during acute HIV-1 infection


title: CD4+ T cell gene expression from Participant P (chip1, lane2) during acute HIV-1 infection


title: CD4-CD8- T cell gene expression from Participant P (chip1, lane1) during acute HIV-1 infection


title: ecto live bacteria non-treated control r1


title: CD4+ T cell gene expression from Participant P (chip1, lane5) during acute HIV-1 infection


title: CD4+ T cell gene expression from Participant O (chip1, lane6) during acute HIV-1 infection

extract_protocol:
Sorted T cells were washed and resuspended in PBS. 21,000 cells were loaded into each well of 8 wells of a Chromium Next GEM Chip G chip and processed for scRNA-seq through the Chromium Controller (both 10x Genomics) instrument work-up per manufacturer's instructions
Library constructions were processed using the Chromium NextGEM Single Cell 5’ v1.1 Kit (10x Genomics) per manufacturer’s instructions


title: CD4+ T cell gene expression from Participant P (chip1, lane3) during acute HIV-1 infection


title: CD4+ T cell gene expression from Participant O (chip1, lane5) during acute HIV-1 infection


title: CD4+ T cell gene expression from Participant O (chip1, lane4) during acute HIV-1 infection


title: CD4-CD8- T cell gene expression from Participant O (chip1, lane1) during acute HIV-1 infection

source_name: CD4-CD8- T cell

value: CD4-CD8- T cell


title: CD4+ T cell gene expression from Participant O (chip1, lane3) during acute HIV-1 infection


title: CD4+ T cell gene expression from Participant O (chip1, lane2) during acute HIV-1 infection


title: Cells stimulated with PBS Sample 16

city: Seattle

first: Donald

last: Nyangahu

state: Washington

address: 307 Westlake Ave N

institute: Seattle Children's Research Institute

data_processing:
Quality filtering was performed using fastp. Reads were mapped onto the human transcriptome by salmon. Quant.sf files were imported into R and processed by tximport package to obtain raw count data file.
Count file was then used for differential gene expression testing by DESEq2.
comma separated file containing gene counts across all samples. Both raw and normalized counts

source_name: Human colonic epithelial cells

extract_protocol:
RNA was extracted using RNeasy mini kit (Qiagen).
RNA sequencing libraries were prepared using NEBNext Ultra II RNA library prep kit for illumina following manufacturer's instructions

value: Human colonic epithelial cells

value: Cells stimulated with PBS

tag: stimulation time


title: Cells stimulated with PBS Sample 17


title: Cells stimulated with Bifidobacterium thetaiotaomicron Sample 11


title: Cells stimulated with heat-killed Bifidobacterium infantis Sample 14


title: Cells stimulated with heat-killed Bifidobacterium infantis Sample 13


title: Cells stimulated with Bifidobacterium thetaiotaomicron Sample 10


title: Cells stimulated with Bifidobacterium infantis derived proteins Sample 8


title: Cells stimulated with Bifidobacterium infantis derived proteins Sample 9


title: Cells stimulated with Bifidobacterium thetaiotaomicron Sample 12


title: Cells stimulated with Bifidobacterium infantis derived proteins Sample 7


title: Cells stimulated with Bifidobacterium infantis derived metabolites Sample 6


title: CD4+ T cell gene expression from Participant O (chip1, lane7) during acute HIV-1 infection


title: Cells stimulated with Bifidobacterium infantis Sample 1


title: Cells stimulated with Bifidobacterium infantis Sample 2


title: Participant F (chip3, lane8) during acute HIV-1 infection

source_name: PBMC

extract_protocol:
PBMC were washed and resuspended in PBS. 21,000 cells were loaded into each well for each of 3 Chromium Next GEM Chip G chips and processed for scRNA-seq through the Chromium Controller (both 10x Genomics) instrument work-up per manufacturer's instructions
Library constructions were processed using the Chromium NextGEM Single Cell 5’ v1.1 Kit (10x Genomics) per manufacturer’s instructions

value: PBMC


title: Participant F (chip3, lane7) during acute HIV-1 infection


title: Participant F (chip3, lane6) during acute HIV-1 infection


title: CD4+ T cell gene expression from Participant O (chip1, lane8) during acute HIV-1 infection


title: Cells stimulated with PBS Sample 18


title: Cells stimulated with Bifidobacterium infantis Sample 3


title: Participant F (chip3, lane3) during acute HIV-1 infection


title: Cells stimulated with Bifidobacterium infantis derived metabolites Sample 4


title: Participant F (chip3, lane2) during acute HIV-1 infection


title: Participant F (chip2, lane8) during acute HIV-1 infection


title: Cells stimulated with heat-killed Bifidobacterium infantis Sample 15


title: Participant F (chip3, lane4) during acute HIV-1 infection


title: Participant F (chip3, lane1) during acute HIV-1 infection


title: Participant M (chip2, lane7) during acute HIV-1 infection


title: Participant F (chip2, lane6) during acute HIV-1 infection


title: Participant F (chip3, lane5) during acute HIV-1 infection


title: Cells stimulated with Bifidobacterium infantis derived metabolites Sample 5


title: Participant I (chip2, lane1) during acute HIV-1 infection


title: Participant H (chip1, lane8) during acute HIV-1 infection


title: Participant F (chip1, lane6) during acute HIV-1 infection


title: Participant N (chip2, lane4) during acute HIV-1 infection


title: Participant K (chip2, lane2) during acute HIV-1 infection


title: Participant J (chip2, lane3) during acute HIV-1 infection


title: Participant G (chip1, lane7) during acute HIV-1 infection


title: Participant E (chip1, lane5) during acute HIV-1 infection


title: Participant C (chip1, lane3) during acute HIV-1 infection


title: Participant D (chip1, lane4) during acute HIV-1 infection


title: Participant B (chip1, lane2) during acute HIV-1 infection


title: vag bacteria supernatant NYC control r2

library_source: genomic

data_processing:
Illumina pipeline for basecalling and fastq demultiplexing
Iterative peak calling from fastq files with the PEPATAC Pipeline (v0.10.3, https://pepatac.databio.org/en/latest/)
Assembly: hg38
Supplementary files format and content: gzipped bed files for peaks called for each sample

molecule: genomic DNA

extract_protocol:
Nuclei were extracted by centrifuging the cells at 500 × g for 10 minutes at 4°C, washing with cold 1x PBS and centrifuging again.  50,000 cells were lysed using cold ATAC lysis + Digitonin (2%). Immediately after lysis, nuclei were spun at 500 × g for 10 minutes
ATAC-seq libraries were generated using the ATAC-seq Kit from Diagenode (Diagenode, A Hologic Company) according to manufacturer instructions

treatment_protocol: Ectocervical, Endocervical and Vaginal cells were exposed to bacterial supernatants for 24 hours.

tag: strain


title: vag LC bacteria supernatants r3


title: vag bacteria supernatant NYC control r1


title: Participant A (chip1, lane1) during acute HIV-1 infection


title: vag LC bacteria supernatants r2


title: vag LC bacteria supernatants r1


title: Participant L (chip2, lane5) during acute HIV-1 infection


title: endo bacteria supernatant NYC control r3


title: endo bacteria supernatant NYC control r2


title: endo bacteria supernatant NYC control r1


title: endo LC bacteria supernatants r3


title: endo LC bacteria supernatants r2


title: endo LC bacteria supernatants r1


title: ecto bacteria supernatant NYC control r3


title: ecto LC bacteria supernatants r2


title: ecto bacteria supernatant NYC control r2


title: ecto LC bacteria supernatants r1


title: ecto LC bacteria supernatants r3


title: ecto bacteria supernatant NYC control r1


title: vag bacteria supernatant NYC control r3


title: UV213

city: Saint Louis

first: Joseph

middle: B

last: Lin

state: MO

address: 660 South Euclid Ave, MSC-8096-06-07

department: Ophthalmology & Visual Sciences

institute: Washington University School of Medicine

description:
AC_uveitis_metadata.csv
AC_uveitis_countmatrix.csv

data_processing:
We processed the raw FASTQ sequencing files using CellRanger with alignment to the 10x Genomics human reference genome (refdata-gex-GRCh38-2020-A). We imported the filtered count matrices into Seurat, and assigned each cell a unique identifier to prevent overlap of barcodes between different samples. We normalized, log-transformed, and scaled the count matrices to remove unwanted sources of variation such as discrepancies in sequencing depth. We identified the top 2000 highly variable genes for principal component analysis. We integrated across samples to account for any sample- or experiment-specific batch effects using the Harmony package. We utilized these harmony embeddings to run Uniform Manifold Approximation and Projection dimensional reduction to 2 dimensions. We clustered cells according to the Louvain algorithm and identified marker genes for each cluster using Wilcoxon rank sum tests comparing each cluster to all other clusters.
Assembly: refdata-gex-GRCh38-2020-A
Supplementary files format and content: AC_uveitis_metadata.csv: Cell-level metadata including sample ID, cell type, and UMAP coordinates (containing all samples).
Supplementary files format and content: AC_uveitis_countmatrix.csv: Raw count matrix (containing all samples).

molecule: polyA RNA

source_name: aqueous humor immune cells

extract_protocol:
We sampled 100-200 ul of the inflamed anterior chamber fluid using a 25- to 30-gauge needle. We pelleted cells by centrifugation (400 x g, 5 min) to remove the aqueous fluid and cryopreserved cells in fetal bovine serum containing 10% DMSO.
We used the 10x Genomics 5'v2 gene expression kit according to the manufacturer's protocol.

value: aqueous humor immune cells

tag: subject status

value: patients with uveitis


title: UV215-003


title: UV195


title: UV176


title: UV179


title: UV170


title: UV180


title: UV150


title: UV143


title: UV129


title: UV132-001


title: UV165


title: UV174


title: UV160


title: UV122


title: UV031


title: UV027


title: UV029


title: UV229


title: GRB52 Embryonic adrenal

city: Dresden

first: Gerard

last: Ruiz-Babot

address: Fetchestrasse 74

country: Germany

institute: Uniklinikum Dresden

data_processing:
Raw paired-end fastq files were preprocessed to remove the low quality reads using TrimGalore
Quality control of the reads was performed prior and after trimming using fastQC and multiQC
Validated reads were aligned using Star against human reference genome and the annotation GTF file (GRCh38.p13) from Gencode v39 primary assembly.
The strandness of the libraries was determined using Rseq and BEDOPS tools and the script infer-experiment.py.
The final matrix of raw counts was formatted using R v4.1.2 and Rstudio GUI
Assembly: GRCh38.p13
Supplementary files format and content: Normalized TPM

source_name: Fetal Adrenal CS21

extract_protocol: Messenger RNA was purified using TRI®Reagent (Sigma, # T9424) using the Direct-zolTM RNA kit (Zymo Research), following the manufacturer’s instructions.

value: Fetal Adrenal CS21


title: GRB4 Adult adrenal

source_name: Human Adult adrenal Female

value: Human Adult adrenal Female


title: GRB6 Adult adrenal


title: UV196


title: GRB7 Embryonic adrenal


title: UV284


title: GRB3 Adult adrenal

source_name: Human Adult adrenal Male

value: Human Adult adrenal Male


title: GRB10 Embryonic adrenal


title: GRB2 Adult adrenal


title: GRB5 Adult adrenal


title: GRB26 hiSCs cell line

source_name: BJFF P29

value: BJFF P29

value: 48 hours with 5 mM br-cAMP


title: GRB18 hiSCs cell line


title: GRB30 hiSCs cell line

source_name: H9 P34 WT

value: H9 P34 WT


title: GRB42 hiSCs cell line

source_name: BJFF

value: BJFF


title: GRB1 Adult adrenal


title: GRB34 hiSCs cell line


title: UV019


title: GRB51 Embryonic adrenal


title: UV186


title: GRB38 hiSCs cell line


title: GRB9 Embryonic adrenal


title: GRB8 Embryonic adrenal


title: GRB25 hiSCs cell line

value: No treated


title: GRB21 hiSCs cell line

source_name: ACS-1101 iPSC

value: ACS-1101 iPSC


title: GRB14 hiSCs cell line


title: GRB22 hiSCs cell line


title: GRB13 hiSCs cell line


title: GRB17 hiSCs cell line


title: GRB33 hiSCs cell line


title: GRB37 hiSCs cell line


title: GRB40 RFP cell line

value: RFP line+br-cAMP


title: GRB41 hiSCs cell line


title: GRB29 hiSCs cell line


title: GRB36 RFP cell line


title: GRB32 RFP cell line


title: GRB24 RFP cell line


title: GRB28 RFP cell line


title: GRB20 RFP cell line


title: GRB27 RFP cell line

value: RFP line


title: GRB23 RFP cell line


title: GRB39 RFP cell line


title: GRB12 RFP cell line


title: GRB31 RFP cell line


title: GRB16 RFP cell line


title: GRB19 RFP cell line


title: GRB35 RFP cell line


title: GRB50 d0 Undifferentiated

value: PSCs


title: GRB15 RFP cell line


title: GRB49 d0 Undifferentiated


title: GRB45 d0 Undifferentiated


title: GRB46 d0 Undifferentiated


title: GRB47 d0 Undifferentiated


title: GRB43 d0 Undifferentiated


title: GRB44 d0 Undifferentiated


title: opn4.CGCTAATTGC-GAAGCAAGCG

city: St Louis

first: David

last: Gutmann

address: 660 S Euclid Ave

department: Neurology

institute: Washington University in St Louis

data_processing:
Partek Flow v10.0
RNA sequencing reads were aligned to the hg38 Ensembl release 105 assembly with STAR v2.7.8a using Partek Flow
Normalization size factors were calculated for all gene counts by median ratio using Partek Flow
Differential genetic analysis was then performed using DESeq2 to analyze for differences between conditions in Partek Flow
Results were filtered for only those genes with false discovery rates ≤ .05 and fold changes more extreme than ± 5
Assembly: hg38
Supplementary files format and content: tab-delimited text file with normalized counts for each sample

source_name: microglia

growth_protocol: human induced microglia (hiMG) were derived from two NF1-mutant human induced pluripotent stem cell (hiPSC) lines as well as an isogenic control line following the published protocol by McQuade et al. (2018)

extract_protocol: NucleoSpin® RNA Plus kit

value: microglia

value: OPG


title: gn4.AATCTGCAGT-ACCTACCAGG

value: GTDUP


title: gn2.AACTGTACGG-ATAACGACGA


title: opn3.AATGTTCGGA-ACGATTGGCT


title: opn1.CGGACAGCTA-GCCTTCCTCT


title: opn2.GCTAACGGCA-GCAACTCCTG


title: bn4.CGCTGTTGAC-ACGTCTACGA

value: WT


title: bn3.GTGAAGACAC-CTTAGGCAAC


title: gn1.AGGCGAATTC-TATGACACGT


title: bn1.TCGGAGACCT-AGTAGCTAGT


title: bn2.AATGCGATGG-GCACACAGCA


title: 3603_OF2B_8-6-D8-P24

city: La Jolla

middle: R

last: Ecker

address: 10010 North Torrey Pines Road

department: Genomic Analysis Laboratory

institute: HHMI-Salk-Institute

data_processing:
Reads trimmed with Cutadapt for adapters and low quality bases (Phred score >= 20)
The resulting files are mapped to in-silico bisulfite converted hg38 reference genome using Hisat-3n.
The raw BAM files were filtered using samtools to keep only uniquely mapped reads and use Picard to remove PCR duplicates.
From the filtered BAM file, DNA reads are selected by read-level mCH base coverage >= 3 and read-level mCH fraction < 0.5, selected reads will be processed by ALLCools package to produce ALLC files (allcools allc-to-bam function). The ALLC files are Tab-delimited files describing the quantity of unmethylated and methylated cytosine base calls.
Trimmed reads will also be mapped by Hisat2 to map to hg38 reference genome with GENCODE human v37 gene annotation. RNA reads are selected by read-level mCH base coverage >= 3 and read-level mCH fraction > 0.9, selected reads will be processed by featureCount to quantify gene level (exon + intron) RNA counts.
Assembly: hg38
Supplementary files format and content: tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls. Columns are: 1. Chromosome, 2. Position, 3. Strand, 4. Cytosin Context, 5. methylated base call, 6. total mapped base call, 7. not used, place holder column.
Library strategy: snmCT-seq

source_name: OF2B human BA46

extract_protocol: Library prepared following protocol discribed in Luo, Chongyuan, Hanqing Liu, Fangming Xie, Ethan J. Armand, Kimberly Siletti, Trygve E. Bakken, Rongxin Fang, et al. 2019. “Single Nucleus Multi-Omics Links Human Cortical Cell Regulatory Genome Diversity to Disease Risk Variants.” bioRxiv. https://doi.org/10.1101/2019.12.11.873398. https://www.protocols.io/view/snmcat-v1-bwubpesn

tag: brain region

value: Dorsolateral Prefrontal cortex (Brodmann area 46)

tag: age

tag: Sex

value: Female

tag: molecule subtype

value: nuclear RNA + genomic DNA


title: GRB11 RFP cell line

tag: cell type


title: 3603_OF2B_8-6-D8-P12


title: 3603_OF2B_8-6-D8-P11


title: 3603_OF2B_8-6-D8-O23


title: 3603_OF2B_8-6-D8-N12


title: 3603_OF2B_8-6-D8-N11


title: 3603_OF2B_8-6-D8-O12


title: 3603_OF2B_8-6-D8-N24


title: 3603_OF2B_8-6-D8-M11


title: 3603_OF2B_8-6-D8-M12


title: 3603_OF2B_8-6-D8-N23


title: 3603_OF2B_8-6-D8-M24


title: 3603_OF2B_8-6-D8-L24


title: 3603_OF2B_8-6-D8-L23


title: 3603_OF2B_8-6-D8-M23


title: 3603_OF2B_8-6-D8-K24


title: 3603_OF2B_8-6-D8-L11


title: 3603_OF2B_8-6-D8-K23


title: 3603_OF2B_8-6-D8-K12


title: 3603_OF2B_8-6-D8-J24


title: 3603_OF2B_8-6-D8-J23


title: 3603_OF2B_8-6-D8-K11


title: 3603_OF2B_8-6-D8-P23


title: 3603_OF2B_8-6-D8-L12


title: 3603_OF2B_8-6-D8-O24


title: 3603_OF2B_8-6-D8-H24


title: 3603_OF2B_8-6-D8-I12


title: 3603_OF2B_8-6-D8-I11


title: 3603_OF2B_8-6-D8-I23


title: 3603_OF2B_8-6-D8-O11


title: 3603_OF2B_8-6-D8-H12


title: 3603_OF2B_8-6-D8-G23


title: 3603_OF2B_8-6-D8-H11


title: 3603_OF2B_8-6-D8-H23


title: 3603_OF2B_8-6-D8-G12


title: 3603_OF2B_8-6-D8-G11


title: 3603_OF2B_8-6-D8-F23


title: 3603_OF2B_8-6-D8-F11


title: 3603_OF2B_8-6-D8-F12


title: 3603_OF2B_8-6-D8-E23


title: 3603_OF2B_8-6-D8-J12


title: 3603_OF2B_8-6-D8-J11


title: 3603_OF2B_8-6-D8-E11


title: 3603_OF2B_8-6-D8-D23


title: 3603_OF2B_8-6-D8-D24


title: 3603_OF2B_8-6-D8-I24


title: 3603_OF2B_8-6-D8-D11


title: 3603_OF2B_8-6-D8-D12


title: 3603_OF2B_8-6-D8-C23


title: 3603_OF2B_8-6-D8-E24


title: 3603_OF2B_8-6-D8-C24


title: 3603_OF2B_8-6-D8-C12


title: 3603_OF2B_8-6-D8-G24


title: 3603_OF2B_8-6-D8-B24


title: 3603_OF2B_8-6-D8-C11


title: 3603_OF2B_8-6-D8-B23


title: 3603_OF2B_8-6-D8-B11


title: 3603_OF2B_8-6-D8-B12


title: 3603_OF2B_8-6-D8-F24


title: 3603_OF2B_8-6-D8-A12


title: 3603_OF2B_8-6-D8-A24


title: 3603_OF2B_8-6-D8-A11


title: 3603_OF2B_8-5-D8-P21


title: 3603_OF2B_8-6-D8-A23


title: 3603_OF2B_8-5-D8-P22


title: 3603_OF2B_8-5-D8-P9


title: 3603_OF2B_8-5-D8-O21


title: 3603_OF2B_8-5-D8-P10


title: 3603_OF2B_8-5-D8-O9


title: 3603_OF2B_8-5-D8-O22


title: 3603_OF2B_8-6-D8-E12


title: 3603_OF2B_8-5-D8-N9


title: 3603_OF2B_8-5-D8-N22


title: 3603_OF2B_8-5-D8-O10


title: 3603_OF2B_8-5-D8-N10


title: 3603_OF2B_8-5-D8-M10


title: 3603_OF2B_8-5-D8-M9


title: 3603_OF2B_8-5-D8-N21


title: 3603_OF2B_8-5-D8-L9


title: 3603_OF2B_8-5-D8-L22


title: 3603_OF2B_8-5-D8-L21


title: 3603_OF2B_8-5-D8-M21


title: 3603_OF2B_8-5-D8-L10


title: 3603_OF2B_8-5-D8-M22


title: 3603_OF2B_8-5-D8-J10


title: 3603_OF2B_8-5-D8-K9


title: 3603_OF2B_8-5-D8-K21


title: 3603_OF2B_8-5-D8-K10


title: 3603_OF2B_8-5-D8-J9


title: 3603_OF2B_8-5-D8-K22


title: 3603_OF2B_8-5-D8-J22


title: 3603_OF2B_8-5-D8-I21


title: 3603_OF2B_8-5-D8-I22


title: 3603_OF2B_8-5-D8-J21


title: 3603_OF2B_8-5-D8-H21


title: 3603_OF2B_8-5-D8-I9


title: 3603_OF2B_8-5-D8-H9


title: 3603_OF2B_8-5-D8-H22


title: 3603_OF2B_8-5-D8-G21


title: 3603_OF2B_8-5-D8-G22


title: 3603_OF2B_8-5-D8-G9


title: 3603_OF2B_8-5-D8-H10


title: 3603_OF2B_8-5-D8-F9


title: 3603_OF2B_8-5-D8-F21


title: 3603_OF2B_8-5-D8-F10


title: 3603_OF2B_8-5-D8-E22


title: 3603_OF2B_8-5-D8-E9


title: 3603_OF2B_8-5-D8-E21


title: 3603_OF2B_8-5-D8-G10


title: 3603_OF2B_8-5-D8-D9


title: 3603_OF2B_8-5-D8-C10


title: 3603_OF2B_8-5-D8-D22


title: 3603_OF2B_8-5-D8-E10


title: 3603_OF2B_8-5-D8-D21


title: 3603_OF2B_8-5-D8-I10


title: 3603_OF2B_8-5-D8-C9


title: 3603_OF2B_8-5-D8-C21


title: 3603_OF2B_8-5-D8-B9


title: 3603_OF2B_8-5-D8-C22


title: 3603_OF2B_8-5-D8-A22


title: 3603_OF2B_8-5-D8-A9


title: 3603_OF2B_8-5-D8-F22


title: 3603_OF2B_8-5-D8-B21


title: 3603_OF2B_8-5-D8-B10


title: 3603_OF2B_8-4-D8-P8


title: 3603_OF2B_8-4-D8-N20


title: 3603_OF2B_8-4-D8-O19


title: 3603_OF2B_8-4-D8-N8


title: 3603_OF2B_8-5-D8-D10


title: 3603_OF2B_8-5-D8-B22


title: 3603_OF2B_8-4-D8-M7


title: 3603_OF2B_8-4-D8-M20


title: 3603_OF2B_8-4-D8-L8


title: 3603_OF2B_8-4-D8-M8


title: 3603_OF2B_8-4-D8-L20


title: 3603_OF2B_8-4-D8-L7


title: 3603_OF2B_8-4-D8-N19


title: 3603_OF2B_8-4-D8-L19


title: 3603_OF2B_8-4-D8-K7


title: 3603_OF2B_8-4-D8-O20


title: 3603_OF2B_8-4-D8-J8


title: 3603_OF2B_8-4-D8-J19


title: 3603_OF2B_8-4-D8-J7


title: 3603_OF2B_8-4-D8-I8


title: 3603_OF2B_8-4-D8-I20


title: 3603_OF2B_8-4-D8-O8


title: 3603_OF2B_8-5-D8-A21


title: 3603_OF2B_8-5-D8-A10


title: 3603_OF2B_8-4-D8-N7


title: 3603_OF2B_8-4-D8-O7


title: 3603_OF2B_8-4-D8-M19


title: 3603_OF2B_8-4-D8-I19


title: 3603_OF2B_8-4-D8-P20


title: 3603_OF2B_8-4-D8-G8


title: 3603_OF2B_8-4-D8-H7


title: 3603_OF2B_8-4-D8-G19


title: 3603_OF2B_8-4-D8-K8


title: 3603_OF2B_8-4-D8-K19


title: 3603_OF2B_8-4-D8-P7


title: 3603_OF2B_8-4-D8-J20


title: 3603_OF2B_8-4-D8-P19


title: 3603_OF2B_8-4-D8-E8


title: 3603_OF2B_8-4-D8-F19


title: 3603_OF2B_8-4-D8-I7


title: 3603_OF2B_8-4-D8-F7


title: 3603_OF2B_8-4-D8-K20


title: 3603_OF2B_8-4-D8-D8


title: 3603_OF2B_8-4-D8-H8


title: 3603_OF2B_8-4-D8-H19


title: 3603_OF2B_8-4-D8-D20


title: 3603_OF2B_8-4-D8-D19


title: 3603_OF2B_8-4-D8-H20


title: 3603_OF2B_8-4-D8-G7


title: 3603_OF2B_8-4-D8-C7


title: 3603_OF2B_8-4-D8-G20


title: 3603_OF2B_8-4-D8-E7


title: 3603_OF2B_8-4-D8-B8


title: 3603_OF2B_8-4-D8-D7


title: 3603_OF2B_8-4-D8-E19


title: 3603_OF2B_8-4-D8-F8


title: 3603_OF2B_8-4-D8-C8


title: 3603_OF2B_8-4-D8-C20


title: 3603_OF2B_8-4-D8-C19


title: 3603_OF2B_8-4-D8-F20


title: 3603_OF2B_8-4-D8-A19


title: 3603_OF2B_8-4-D8-B7


title: 3603_OF2B_8-4-D8-B20


title: 3603_OF2B_8-3-D8-P6


title: 3603_OF2B_8-4-D8-A8


title: 3603_OF2B_8-4-D8-B19


title: 3603_OF2B_8-3-D8-O6


title: 3603_OF2B_8-3-D8-P17


title: 3603_OF2B_8-4-D8-A7


title: 3603_OF2B_8-3-D8-P5


title: 3603_OF2B_8-3-D8-P18


title: 3603_OF2B_8-3-D8-O5


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