title: heart biopsy: 1581


title: heart biopsy: 1580

status: Public on Apr 02 2024

type: RNA

city: Edmonton

first: Jessica

last: Chang

address: 250 Heritage Medical Research Centre

department: Medicine

country: Canada

institute: University of Alberta

hyb_protocol: (Affymetrix, Santa Clara, CA).  Amplified RNA (10ug) was hybridized for 16 hr at 45C on  GeneChip® PrimeView™ Human Gene Expression Array . GeneChips were washed and stained in the Affymetrix Fluidics Station 450.

scan_protocol: GeneChips were scanned using Gene Array Scanner 3000 7G (Affymetrix) and processed with GeneChip® Command Console® Software (AGCC)(Affymetrix).

data_processing: Microarray data files were preprocessed using robust multi-chip averaging (RMA) implemented in Bioconductor.

label: biotin

molecule: total RNA

organism: Homo sapiens

source_name: heart biopsy

label_protocol: Total RNA (0.5µg) was labeled using GeneChip 3` IVT Express Labeling kit. Quality of labeled aRNA was assessed on an Agilent 2100 Bioanalyzer (Agilent, Palo Alto, CA) before hybridization to GeneChip® PrimeView™ Human Gene Expression Array

growth_protocol: All human heart biopsies taken for clinical indication during the period specified above were included. Tissue was immediately placed in RNA later.

extract_protocol: Tissue was homogenization in 0.5ml of Trizol reagent (Invitrogen, Carlsbad, CA) and total RNA was extracted and purified using the Rneasy Micro Kit (Quiagen, Ontario, Canada) (average yield 8µg/core, (RNA integrity number >7).

treatment_protocol: The immunosuppressive treatment of the patients before the biopsy was based on individual treatment regiments; the treatment after the biopsy was adjusted based on the histopathological diagnosis.

tag: tissue

value: heart biopsy

tag: lowlvef (yes, no)

value: no


title: heart biopsy: 1579


title: heart biopsy: 1578


title: heart biopsy: 1575


title: heart biopsy: 1576


title: heart biopsy: 1577


title: heart biopsy: 1573


title: heart biopsy: 1574


title: heart biopsy: 1571


title: heart biopsy: 1572


title: heart biopsy: 1563


title: heart biopsy: 1567


title: heart biopsy: 1556


title: heart biopsy: 1569


title: heart biopsy: 1557


title: heart biopsy: 1562


title: heart biopsy: 1560


title: heart biopsy: 1583


title: heart biopsy: 1570


title: heart biopsy: 1568


title: heart biopsy: 1555


title: heart biopsy: 2029


title: heart biopsy: 1565


title: heart biopsy: 1554


title: heart biopsy: 1559


title: heart biopsy: 1558


title: heart biopsy: 1564


title: heart biopsy: 1566


title: heart biopsy: 1550


title: heart biopsy: 1582


title: heart biopsy: 1549


title: heart biopsy: 1551


title: heart biopsy: 1546


title: heart biopsy: 1548


title: heart biopsy: 1547


title: heart biopsy: 1561


title: heart biopsy: 1553


title: heart biopsy: 1552


title: heart biopsy: 1541


title: heart biopsy: 1536


title: heart biopsy: 1539


title: heart biopsy: 1540


title: heart biopsy: 1545


title: heart biopsy: 1534


title: heart biopsy: 1533


title: heart biopsy: 1530


title: heart biopsy: 1544


title: heart biopsy: 1535


title: heart biopsy: 1529


title: heart biopsy: 1532


title: heart biopsy: 1528


title: heart biopsy: 1543


title: heart biopsy: 1531


title: heart biopsy: 1525


title: heart biopsy: 1527


title: heart biopsy: 1537


title: heart biopsy: 1526


title: heart biopsy: 1524


title: heart biopsy: 1523


title: heart biopsy: 1522


title: heart biopsy: 1542


title: heart biopsy: 1519


title: heart biopsy: 1538


title: heart biopsy: 1521


title: heart biopsy: 1518


title: heart biopsy: 1520


title: heart biopsy: 1517


title: heart biopsy: 1516


title: heart biopsy: 1515


title: heart biopsy: 1513


title: heart biopsy: 1512


title: heart biopsy: 1511


title: heart biopsy: 1505


title: heart biopsy: 1502


title: heart biopsy: 1497


title: heart biopsy: 1506


title: heart biopsy: 1493


title: heart biopsy: 1500


title: heart biopsy: 1514


title: heart biopsy: 1509


title: heart biopsy: 1494


title: heart biopsy: 1503


title: heart biopsy: 1501


title: heart biopsy: 1510


title: heart biopsy: 1495


title: heart biopsy: 1504


title: heart biopsy: 1498


title: heart biopsy: 1496


title: heart biopsy: 1489


title: heart biopsy: 1490


title: heart biopsy: 1499


title: heart biopsy: 1492


title: heart biopsy: 1491


title: heart biopsy: 1487


title: heart biopsy: 1485


title: heart biopsy: 1508


title: heart biopsy: 1507


title: heart biopsy: 1486


title: heart biopsy: 1484


title: heart biopsy: 1481


title: heart biopsy: 1488


title: heart biopsy: 1480


title: heart biopsy: 1482


title: heart biopsy: 1473


title: heart biopsy: 1479


title: heart biopsy: 1472


title: heart biopsy: 1476


title: heart biopsy: 1467


title: heart biopsy: 1469


title: heart biopsy: 1474


title: heart biopsy: 1466


title: heart biopsy: 1477


title: heart biopsy: 1463


title: heart biopsy: 1475


title: heart biopsy: 1464


title: heart biopsy: 1470


title: heart biopsy: 1465


title: heart biopsy: 1468


title: heart biopsy: 1461


title: heart biopsy: 1462


title: heart biopsy: 1457


title: heart biopsy: 1483


title: heart biopsy: 1456


title: heart biopsy: 1460


title: heart biopsy: 1459


title: heart biopsy: 1455


title: heart biopsy: 1458


title: heart biopsy: 1478


title: heart biopsy: 1454


title: heart biopsy: 1471


title: heart biopsy: 1453


title: heart biopsy: 1441


title: heart biopsy: 1445


title: heart biopsy: 1442


title: heart biopsy: 1447


title: heart biopsy: 1452


title: heart biopsy: 1444


title: heart biopsy: 1448


title: heart biopsy: 1438


title: heart biopsy: 1440


title: heart biopsy: 1437


title: heart biopsy: 1439


title: heart biopsy: 1436


title: heart biopsy: 1435


title: heart biopsy: 1433


title: heart biopsy: 1432


title: heart biopsy: 1431


title: heart biopsy: 1430


title: heart biopsy: 1428


title: heart biopsy: 1451


title: heart biopsy: 1450


title: heart biopsy: 1449


title: heart biopsy: 1446


title: heart biopsy: 1427


title: heart biopsy: 1426


title: heart biopsy: 1423


title: heart biopsy: 1424


title: heart biopsy: 1425


title: heart biopsy: 1413


title: heart biopsy: 1421


title: heart biopsy: 1434


title: heart biopsy: 1414


title: heart biopsy: 1443


title: heart biopsy: 1420


title: heart biopsy: 1418


title: heart biopsy: 1411


title: heart biopsy: 1416


title: heart biopsy: 1417


title: heart biopsy: 1429


title: heart biopsy: 1406


title: heart biopsy: 1409


title: heart biopsy: 1408


title: heart biopsy: 1410


title: heart biopsy: 1407


title: heart biopsy: 1404


title: heart biopsy: 1412


title: heart biopsy: 1403


title: heart biopsy: 1399


title: heart biopsy: 1402


title: heart biopsy: 1401


title: heart biopsy: 1419


title: heart biopsy: 1398


title: heart biopsy: 1422


title: heart biopsy: 1415


title: heart biopsy: 1400


title: heart biopsy: 1396


title: heart biopsy: 1393


title: heart biopsy: 1397


title: heart biopsy: 1391


title: heart biopsy: 1394


title: heart biopsy: 1392


title: heart biopsy: 1395


title: heart biopsy: 1389


title: heart biopsy: 1388


title: heart biopsy: 1387


title: heart biopsy: 1386


title: heart biopsy: 1405


title: heart biopsy: 1385


title: heart biopsy: 1384


title: heart biopsy: 1382


title: heart biopsy: 1383


title: heart biopsy: 1381


title: heart biopsy: 1380


title: heart biopsy: 1379


title: heart biopsy: 1375


title: heart biopsy: 1377


title: heart biopsy: 1378


title: heart biopsy: 1374


title: heart biopsy: 1376


title: heart biopsy: 1390


title: heart biopsy: 1370


title: heart biopsy: 1369


title: heart biopsy: 1373


title: heart biopsy: 1371


title: heart biopsy: 1368


title: heart biopsy: 1372


title: heart biopsy: 1364


title: heart biopsy: 1365


title: heart biopsy: 1367


title: heart biopsy: 1366


title: heart biopsy: 1362


title: heart biopsy: 1361


title: heart biopsy: 1359


title: heart biopsy: 1363


title: heart biopsy: 1358


title: heart biopsy: 1360


title: heart biopsy: 1357


title: heart biopsy: 1356


title: heart biopsy: 1354


title: heart biopsy: 1355


title: heart biopsy: 1351


title: heart biopsy: 1350


title: heart biopsy: 1353


title: heart biopsy: 1349


title: heart biopsy: 1348


title: heart biopsy: 1345


title: heart biopsy: 1346


title: heart biopsy: 1344


title: heart biopsy: 1347


title: heart biopsy: 1341


title: heart biopsy: 1343


title: heart biopsy: 1342


title: heart biopsy: 1338


title: heart biopsy: 1335


title: heart biopsy: 1340


title: heart biopsy: 1332


title: heart biopsy: 1333


title: heart biopsy: 1334


title: heart biopsy: 1336


title: heart biopsy: 1330


title: heart biopsy: 1328


title: heart biopsy: 1352


title: heart biopsy: 1327


title: heart biopsy: 1326


title: heart biopsy: 1325


title: heart biopsy: 1324


title: heart biopsy: 1323


title: heart biopsy: 1322


title: heart biopsy: 1321


title: heart biopsy: 1320


title: heart biopsy: 1318


title: heart biopsy: 1317


title: heart biopsy: 1339


title: heart biopsy: 1319


title: heart biopsy: 1316


title: heart biopsy: 1337


title: heart biopsy: 1312


title: heart biopsy: 1314


title: heart biopsy: 1310


title: heart biopsy: 1331


title: heart biopsy: 1313


title: heart biopsy: 1311


title: heart biopsy: 1307


title: heart biopsy: 1304


title: heart biopsy: 1309


title: heart biopsy: 1329


title: heart biopsy: 1303


title: heart biopsy: 1305


title: heart biopsy: 1302


title: heart biopsy: 1306


title: heart biopsy: 1301


title: heart biopsy: 1299


title: heart biopsy: 1296


title: heart biopsy: 1300


title: heart biopsy: 1297


title: heart biopsy: 1298


title: heart biopsy: 1315


title: heart biopsy: 1294


title: heart biopsy: 1295


title: heart biopsy: 1290


title: heart biopsy: 1291


title: heart biopsy: 1292


title: heart biopsy: 1289


title: heart biopsy: 1287


title: heart biopsy: 1293


title: heart biopsy: 1288


title: heart biopsy: 1308


title: heart biopsy: 1285


title: heart biopsy: 1282


title: heart biopsy: 1284


title: heart biopsy: 1283


title: heart biopsy: 1280


title: heart biopsy: 1278


title: heart biopsy: 1286


title: heart biopsy: 1276


title: heart biopsy: 1277


title: heart biopsy: 1273


title: heart biopsy: 1275


title: heart biopsy: 1281


title: heart biopsy: 1279


title: heart biopsy: 1271


title: heart biopsy: 1270


title: heart biopsy: 1268


title: heart biopsy: 1269


title: heart biopsy: 1272


title: heart biopsy: 1266


title: heart biopsy: 1265


title: heart biopsy: 1264


title: heart biopsy: 1263


title: heart biopsy: 1267


title: heart biopsy: 1260


title: heart biopsy: 1261


title: heart biopsy: 1257


title: heart biopsy: 1256


title: heart biopsy: 1255


title: heart biopsy: 1254


title: heart biopsy: 1253


title: heart biopsy: 1274


title: heart biopsy: 1251


title: heart biopsy: 1262


title: heart biopsy: 1259


title: heart biopsy: 1250


title: heart biopsy: 1249


title: heart biopsy: 1246


title: heart biopsy: 1258


title: heart biopsy: 1247


title: heart biopsy: 1239


title: heart biopsy: 1243


title: heart biopsy: 1244


title: heart biopsy: 1242


title: heart biopsy: 1238


title: heart biopsy: 1235


title: heart biopsy: 1237


title: heart biopsy: 1232


title: heart biopsy: 1233


title: heart biopsy: 1231


title: heart biopsy: 1236


title: heart biopsy: 1234


title: heart biopsy: 1230


title: heart biopsy: 1229


title: heart biopsy: 1252


title: heart biopsy: 1248


title: heart biopsy: 1227


title: heart biopsy: 1225


title: heart biopsy: 1226


title: heart biopsy: 1228


title: heart biopsy: 1223


title: heart biopsy: 1222


title: heart biopsy: 1245


title: heart biopsy: 1224


title: heart biopsy: 1220


title: heart biopsy: 1241


title: heart biopsy: 1240


title: heart biopsy: 1218


title: heart biopsy: 1216


title: heart biopsy: 1215


title: heart biopsy: 1219


title: heart biopsy: 1217


title: heart biopsy: 1214


title: heart biopsy: 1212


title: heart biopsy: 1211


title: heart biopsy: 1210


title: heart biopsy: 1213


title: heart biopsy: 1208


title: heart biopsy: 1209


title: heart biopsy: 1207


title: heart biopsy: 1206


title: heart biopsy: 1205


title: heart biopsy: 1204


title: heart biopsy: 1221


title: heart biopsy: 1199


title: heart biopsy: 1202


title: heart biopsy: 1200


title: heart biopsy: 1198


title: heart biopsy: 1197


title: heart biopsy: 1201


title: heart biopsy: 1203


title: heart biopsy: 1195


title: heart biopsy: 1193


title: heart biopsy: 1192


title: heart biopsy: 1194


title: heart biopsy: 1196


title: heart biopsy: 1190


title: heart biopsy: 1191


title: heart biopsy: 1189


title: heart biopsy: 1186


title: heart biopsy: 1187


title: heart biopsy: 1185


title: heart biopsy: 1181


title: heart biopsy: 1184


title: heart biopsy: 1180


title: heart biopsy: 1188


title: heart biopsy: 1182


title: heart biopsy: 1178


title: heart biopsy: 1176


title: heart biopsy: 1172


title: heart biopsy: 1173


title: heart biopsy: 1169


title: heart biopsy: 1170


title: heart biopsy: 1177


title: heart biopsy: 1174


title: heart biopsy: 1167


title: heart biopsy: 1171


title: heart biopsy: 1165


title: heart biopsy: 1161


title: heart biopsy: 1166


title: heart biopsy: 1163


title: heart biopsy: 1160


title: heart biopsy: 1164


title: heart biopsy: 1162


title: heart biopsy: 1159


title: heart biopsy: 1168


title: heart biopsy: 1153


title: heart biopsy: 1157


title: heart biopsy: 1156


title: heart biopsy: 1155


title: heart biopsy: 1183


title: heart biopsy: 1175


title: heart biopsy: 1150


title: heart biopsy: 1149


title: heart biopsy: 1154


title: heart biopsy: 1151


title: heart biopsy: 1152


title: heart biopsy: 1147


title: heart biopsy: 1148


title: heart biopsy: 1144


title: heart biopsy: 1143


title: heart biopsy: 1142


title: heart biopsy: 1145


title: heart biopsy: 1141


title: heart biopsy: 1140


title: heart biopsy: 1139


title: heart biopsy: 1137


title: heart biopsy: 1138


title: heart biopsy: 1136


title: heart biopsy: 1134


title: heart biopsy: 1135


title: heart biopsy: 1132


title: heart biopsy: 1158


title: heart biopsy: 1127


title: heart biopsy: 1129


title: heart biopsy: 1130


title: heart biopsy: 1126


title: heart biopsy: 1131


title: heart biopsy: 1121


title: heart biopsy: 1122


title: heart biopsy: 1123


title: heart biopsy: 1146


title: heart biopsy: 1179


title: heart biopsy: 1119


title: heart biopsy: 1118


title: heart biopsy: 1117


title: heart biopsy: 1115


title: heart biopsy: 1116


title: heart biopsy: 1112


title: heart biopsy: 1114


title: heart biopsy: 1113


title: heart biopsy: 1110


title: heart biopsy: 1109


title: heart biopsy: 1108


title: heart biopsy: 1106


title: heart biopsy: 1107


title: heart biopsy: 1124


title: heart biopsy: 1128


title: heart biopsy: 1125


title: heart biopsy: 1102


title: heart biopsy: 1105


title: heart biopsy: 1101


title: heart biopsy: 1104


title: heart biopsy: 1133


title: heart biopsy: 1100


title: heart biopsy: 1099


title: heart biopsy: 1098


title: heart biopsy: 1120


title: heart biopsy: 1095


title: heart biopsy: 1096


title: heart biopsy: 1094


title: heart biopsy: 1091


title: heart biopsy: 1092


title: heart biopsy: 1111


title: heart biopsy: 1097


title: heart biopsy: 1090


title: heart biopsy: 1093


title: heart biopsy: 1086


title: heart biopsy: 1088


title: heart biopsy: 1085


title: heart biopsy: 1087


title: heart biopsy: 1103


title: heart biopsy: 1081


title: heart biopsy: 1082


title: heart biopsy: 1080


title: heart biopsy: 1079


title: heart biopsy: 1078


title: heart biopsy: 1077


title: heart biopsy: 1076


title: heart biopsy: 1074


title: heart biopsy: 1073


title: heart biopsy: 1075


title: heart biopsy: 1070


title: heart biopsy: 1069


title: heart biopsy: 1068


title: heart biopsy: 1067


title: heart biopsy: 1089


title: heart biopsy: 1065


title: heart biopsy: 1066


title: heart biopsy: 1064


title: heart biopsy: 1063


title: heart biopsy: 1061


title: heart biopsy: 1084


title: heart biopsy: 1060


title: heart biopsy: 1059


title: heart biopsy: 1058


title: heart biopsy: 1083


title: heart biopsy: 1057


title: heart biopsy: 1056


title: heart biopsy: 1055


title: heart biopsy: 1053


title: heart biopsy: 1050


title: heart biopsy: 1049


title: heart biopsy: 1052


title: heart biopsy: 1072


title: heart biopsy: 1062


title: heart biopsy: 1071


title: heart biopsy: 1054


title: heart biopsy: 1051


title: heart biopsy: 1048


title: heart biopsy: 1047


title: heart biopsy: 1046


title: heart biopsy: 1044


title: heart biopsy: 1045


title: heart biopsy: 1042


title: heart biopsy: 1043


title: heart biopsy: 1040


title: heart biopsy: 1041


title: heart biopsy: 1038


title: heart biopsy: 1037


title: heart biopsy: 1039


title: heart biopsy: 1036


title: heart biopsy: 1035


title: heart biopsy: 1033


title: heart biopsy: 1034


title: heart biopsy: 1032


title: heart biopsy: 1031


title: heart biopsy: 1028


title: heart biopsy: 1027


title: heart biopsy: 1029


title: heart biopsy: 1025


title: heart biopsy: 1024


title: heart biopsy: 1020


title: heart biopsy: 1021


title: heart biopsy: 1022


title: heart biopsy: 1019


title: heart biopsy: 1023


title: heart biopsy: 1018


title: heart biopsy: 1017


title: heart biopsy: 1026


title: heart biopsy: 1015


title: heart biopsy: 1012


title: heart biopsy: 1016


title: heart biopsy: 1011


title: heart biopsy: 1014


title: heart biopsy: 1013


title: heart biopsy: 1010


title: heart biopsy: 1009


title: heart biopsy: 1006


title: heart biopsy: 1005


title: heart biopsy: 1030


title: heart biopsy: 1003


title: heart biopsy: 1001


title: heart biopsy: 1002


title: heart biopsy: 1000


title: heart biopsy: 999


title: heart biopsy: 998


title: heart biopsy: 996


title: heart biopsy: 994


title: heart biopsy: 995


title: heart biopsy: 997


title: heart biopsy: 993


title: heart biopsy: 991


title: heart biopsy: 987


title: heart biopsy: 988


title: heart biopsy: 990


title: heart biopsy: 984


title: heart biopsy: 985


title: heart biopsy: 983


title: heart biopsy: 1008


title: heart biopsy: 986


title: heart biopsy: 1007


title: heart biopsy: 1004


title: heart biopsy: 980


title: heart biopsy: 978


title: heart biopsy: 981


title: heart biopsy: 977


title: heart biopsy: 982


title: heart biopsy: 976


title: heart biopsy: 974


title: heart biopsy: 975


title: heart biopsy: 972


title: heart biopsy: 971


title: heart biopsy: 973


title: heart biopsy: 992


title: heart biopsy: 968


title: heart biopsy: 970


title: heart biopsy: 969


title: heart biopsy: 989


title: heart biopsy: 967


title: heart biopsy: 966


title: heart biopsy: 965


title: heart biopsy: 960


title: heart biopsy: 964


title: heart biopsy: 963


title: heart biopsy: 959


title: heart biopsy: 979


title: heart biopsy: 961


title: heart biopsy: 957


title: heart biopsy: 958


title: heart biopsy: 955


title: heart biopsy: 953


title: heart biopsy: 954


title: heart biopsy: 951


title: heart biopsy: 962


title: heart biopsy: 952


title: heart biopsy: 950


title: heart biopsy: 956


title: heart biopsy: 948


title: heart biopsy: 949


title: heart biopsy: 947


title: heart biopsy: 946


title: heart biopsy: 945


title: heart biopsy: 943


title: heart biopsy: 944


title: heart biopsy: 940


title: heart biopsy: 942


title: heart biopsy: 939


title: heart biopsy: 941


title: heart biopsy: 938


title: heart biopsy: 936


title: heart biopsy: 937


title: heart biopsy: 932


title: heart biopsy: 934


title: heart biopsy: 935


title: heart biopsy: 933


title: heart biopsy: 930


title: heart biopsy: 931


title: heart biopsy: 928


title: heart biopsy: 929


title: heart biopsy: 926


title: heart biopsy: 924


title: heart biopsy: 923


title: heart biopsy: 927


title: heart biopsy: 922


title: heart biopsy: 925


title: heart biopsy: 920


title: heart biopsy: 917


title: heart biopsy: 915


title: heart biopsy: 916


title: heart biopsy: 914


title: heart biopsy: 912


title: heart biopsy: 913


title: heart biopsy: 909


title: heart biopsy: 910


title: heart biopsy: 908


title: heart biopsy: 907


title: heart biopsy: 905


title: heart biopsy: 903


title: heart biopsy: 902


title: heart biopsy: 904


title: heart biopsy: 919


title: heart biopsy: 900


title: heart biopsy: 899


title: heart biopsy: 895


title: heart biopsy: 898


title: heart biopsy: 918


title: heart biopsy: 896


title: heart biopsy: 893


title: heart biopsy: 891


title: heart biopsy: 892


title: heart biopsy: 894


title: heart biopsy: 897


title: heart biopsy: 921


title: heart biopsy: 890


title: heart biopsy: 911


title: heart biopsy: 889


title: heart biopsy: 887


title: heart biopsy: 885


title: heart biopsy: 888


title: heart biopsy: 883


title: heart biopsy: 886


title: heart biopsy: 906


title: heart biopsy: 884


title: heart biopsy: 901


title: heart biopsy: 881


title: heart biopsy: 882


title: heart biopsy: 877


title: heart biopsy: 878


title: heart biopsy: 879


title: heart biopsy: 880


title: heart biopsy: 875


title: heart biopsy: 874


title: heart biopsy: 876


title: heart biopsy: 873


title: heart biopsy: 869


title: heart biopsy: 872


title: heart biopsy: 864


title: heart biopsy: 865


title: heart biopsy: 867


title: heart biopsy: 870


title: heart biopsy: 868


title: heart biopsy: 859


title: heart biopsy: 863


title: heart biopsy: 871


title: heart biopsy: 857


title: heart biopsy: 862


title: heart biopsy: 866


title: heart biopsy: 858


title: heart biopsy: 861


title: heart biopsy: 856


title: heart biopsy: 855


title: heart biopsy: 853


title: heart biopsy: 854


title: heart biopsy: 860


title: heart biopsy: 852


title: heart biopsy: 849


title: heart biopsy: 846


title: heart biopsy: 847


title: heart biopsy: 850


title: heart biopsy: 845


title: heart biopsy: 844


title: heart biopsy: 842


title: heart biopsy: 839


title: heart biopsy: 841


title: heart biopsy: 837


title: heart biopsy: 838


title: heart biopsy: 836


title: heart biopsy: 840


title: heart biopsy: 834


title: heart biopsy: 835


title: heart biopsy: 831


title: heart biopsy: 832


title: heart biopsy: 828


title: heart biopsy: 829


title: heart biopsy: 827


title: heart biopsy: 830


title: heart biopsy: 851


title: heart biopsy: 843


title: heart biopsy: 833


title: heart biopsy: 824


title: heart biopsy: 826


title: heart biopsy: 823


title: heart biopsy: 825


title: heart biopsy: 822


title: heart biopsy: 848


title: heart biopsy: 820


title: heart biopsy: 819


title: heart biopsy: 817


title: heart biopsy: 816


title: heart biopsy: 814


title: heart biopsy: 815


title: heart biopsy: 812


title: heart biopsy: 810


title: heart biopsy: 811


title: heart biopsy: 808


title: heart biopsy: 809


title: heart biopsy: 806


title: heart biopsy: 804


title: heart biopsy: 805


title: heart biopsy: 801


title: heart biopsy: 799


title: heart biopsy: 802


title: heart biopsy: 796


title: heart biopsy: 800


title: heart biopsy: 795


title: heart biopsy: 793


title: heart biopsy: 798


title: heart biopsy: 821


title: heart biopsy: 794


title: heart biopsy: 818


title: heart biopsy: 791


title: heart biopsy: 792


title: heart biopsy: 788


title: heart biopsy: 789


title: heart biopsy: 813


title: heart biopsy: 807


title: heart biopsy: 786


title: heart biopsy: 785


title: heart biopsy: 787


title: heart biopsy: 784


title: heart biopsy: 781


title: heart biopsy: 782


title: heart biopsy: 803


title: heart biopsy: 780


title: heart biopsy: 797


title: heart biopsy: 777


title: heart biopsy: 775


title: heart biopsy: 774


title: heart biopsy: 773


title: heart biopsy: 771


title: heart biopsy: 770


title: heart biopsy: 769


title: heart biopsy: 772


title: heart biopsy: 767


title: heart biopsy: 778


title: heart biopsy: 768


title: heart biopsy: 790


title: heart biopsy: 765


title: heart biopsy: 764


title: heart biopsy: 763


title: heart biopsy: 766


title: heart biopsy: 762


title: heart biopsy: 783


title: heart biopsy: 761


title: heart biopsy: 759


title: heart biopsy: 757


title: heart biopsy: 756


title: heart biopsy: 779


title: heart biopsy: 754


title: heart biopsy: 776


title: heart biopsy: 753


title: heart biopsy: 752


title: heart biopsy: 751


title: heart biopsy: 748


title: heart biopsy: 744


title: heart biopsy: 745


title: heart biopsy: 746


title: heart biopsy: 749


title: heart biopsy: 743


title: heart biopsy: 741


title: heart biopsy: 742


title: heart biopsy: 738


title: heart biopsy: 739


title: heart biopsy: 737


title: heart biopsy: 736


title: heart biopsy: 760


title: heart biopsy: 734


title: heart biopsy: 735


title: heart biopsy: 755


title: heart biopsy: 733


title: heart biopsy: 740


title: heart biopsy: 731


title: heart biopsy: 732


title: heart biopsy: 758


title: heart biopsy: 729


title: heart biopsy: 730


title: heart biopsy: 727


title: heart biopsy: 750


title: heart biopsy: 728


title: heart biopsy: 747


title: heart biopsy: 726


title: heart biopsy: 719


title: heart biopsy: 725


title: heart biopsy: 723


title: heart biopsy: 724


title: heart biopsy: 721


title: heart biopsy: 722


title: heart biopsy: 720


title: heart biopsy: 717


title: heart biopsy: 713


title: heart biopsy: 716


title: heart biopsy: 718


title: heart biopsy: 712


title: heart biopsy: 711


title: heart biopsy: 715


title: heart biopsy: 714


title: heart biopsy: 710


title: heart biopsy: 709


title: heart biopsy: 707


title: heart biopsy: 705


title: heart biopsy: 703


title: heart biopsy: 706


title: heart biopsy: 708


title: heart biopsy: 701


title: heart biopsy: 702


title: heart biopsy: 700


title: heart biopsy: 698


title: heart biopsy: 697


title: heart biopsy: 699


title: heart biopsy: 696


title: heart biopsy: 694


title: heart biopsy: 691


title: heart biopsy: 690


title: heart biopsy: 695


title: heart biopsy: 693


title: heart biopsy: 692


title: heart biopsy: 689


title: heart biopsy: 681


title: heart biopsy: 685


title: heart biopsy: 686


title: heart biopsy: 688


title: heart biopsy: 683


title: heart biopsy: 704


title: heart biopsy: 682


title: heart biopsy: 684


title: heart biopsy: 680


title: heart biopsy: 679


title: heart biopsy: 678


title: heart biopsy: 674


title: heart biopsy: 675


title: heart biopsy: 676


title: heart biopsy: 671


title: heart biopsy: 672


title: heart biopsy: 673


title: heart biopsy: 670


title: heart biopsy: 669


title: heart biopsy: 668


title: heart biopsy: 667


title: heart biopsy: 666


title: heart biopsy: 665


title: heart biopsy: 687


title: heart biopsy: 663


title: heart biopsy: 662


title: heart biopsy: 660


title: heart biopsy: 1609

value: yes


title: heart biopsy: 1608


title: heart biopsy: 1607


title: heart biopsy: 658


title: heart biopsy: 1603


title: heart biopsy: 659


title: heart biopsy: 1606


title: heart biopsy: 677


title: heart biopsy: 1602


title: heart biopsy: 1604


title: heart biopsy: 1597


title: heart biopsy: 1600


title: heart biopsy: 1595


title: heart biopsy: 1599


title: heart biopsy: 1596


title: heart biopsy: 1601


title: heart biopsy: 664


title: heart biopsy: 1593


title: heart biopsy: 1598


title: heart biopsy: 1591


title: heart biopsy: 1590


title: heart biopsy: 1592


title: heart biopsy: 661


title: heart biopsy: 2399


title: heart biopsy: 2393


title: heart biopsy: 2392


title: heart biopsy: 1594


title: heart biopsy: 1605


title: heart biopsy: 657


title: heart biopsy: 2354


title: heart biopsy: 2340


title: heart biopsy: 2374


title: heart biopsy: 2373


title: heart biopsy: 2353


title: heart biopsy: 2341


title: heart biopsy: 2337


title: heart biopsy: 2376


title: heart biopsy: 2375


title: heart biopsy: 2329


title: heart biopsy: 2331


title: heart biopsy: 2336


title: heart biopsy: 2335


title: heart biopsy: 2435


title: heart biopsy: 2332


title: heart biopsy: 2328


title: heart biopsy: 2326


title: heart biopsy: 2325


title: heart biopsy: 2323


title: heart biopsy: 2314


title: heart biopsy: 2302


title: heart biopsy: 2315


title: heart biopsy: 2303


title: heart biopsy: 2287


title: heart biopsy: 2316


title: heart biopsy: 2300


title: heart biopsy: 2281


title: heart biopsy: 2295


title: heart biopsy: 2280


title: heart biopsy: 2279


title: heart biopsy: 2301


title: heart biopsy: 2296


title: heart biopsy: 2277


title: heart biopsy: 2278


title: heart biopsy: 2273


title: heart biopsy: 2274


title: heart biopsy: 2272


title: heart biopsy: 2276


title: heart biopsy: 2268


title: heart biopsy: 2263


title: heart biopsy: 2262


title: heart biopsy: 2270


title: heart biopsy: 2264


title: heart biopsy: 2259


title: heart biopsy: 2256


title: heart biopsy: 2254


title: heart biopsy: 2253


title: heart biopsy: 2252


title: heart biopsy: 2249


title: heart biopsy: 2275


title: heart biopsy: 2250


title: heart biopsy: 2246


title: heart biopsy: 2245


title: heart biopsy: 2248


title: heart biopsy: 2266


title: heart biopsy: 2260


title: heart biopsy: 2242


title: heart biopsy: 2239


title: heart biopsy: 2244


title: heart biopsy: 2238


title: heart biopsy: 2261


title: heart biopsy: 2236


title: heart biopsy: 2235


title: heart biopsy: 2233


title: heart biopsy: 2234


title: heart biopsy: 2232


title: heart biopsy: 2230


title: heart biopsy: 2231


title: heart biopsy: 2229


title: heart biopsy: 2227


title: heart biopsy: 2228


title: heart biopsy: 2251


title: heart biopsy: 2226


title: heart biopsy: 2224


title: heart biopsy: 2225


title: heart biopsy: 2220


title: heart biopsy: 2221


title: heart biopsy: 2241


title: heart biopsy: 2240


title: heart biopsy: 2219


title: heart biopsy: 2218


title: heart biopsy: 2223


title: heart biopsy: 2217


title: heart biopsy: 2214


title: heart biopsy: 2216


title: heart biopsy: 2213


title: heart biopsy: 2237


title: heart biopsy: 2210


title: heart biopsy: 2208


title: heart biopsy: 2207


title: heart biopsy: 2211


title: heart biopsy: 2209


title: heart biopsy: 2204


title: heart biopsy: 2203


title: heart biopsy: 2202


title: heart biopsy: 2206


title: heart biopsy: 2201


title: heart biopsy: 2205


title: heart biopsy: 2200


title: heart biopsy: 2195


title: heart biopsy: 2199


title: heart biopsy: 2194


title: heart biopsy: 2196


title: heart biopsy: 2193


title: heart biopsy: 2192


title: heart biopsy: 2189


title: heart biopsy: 2190


title: heart biopsy: 2188


title: heart biopsy: 2187


title: heart biopsy: 2184


title: heart biopsy: 2198


title: heart biopsy: 2186


title: heart biopsy: 2183


title: heart biopsy: 2180


title: heart biopsy: 2178


title: heart biopsy: 2179


title: heart biopsy: 2181


title: heart biopsy: 2176


title: heart biopsy: 2182


title: heart biopsy: 2191


title: heart biopsy: 2171


title: heart biopsy: 2174


title: heart biopsy: 2173


title: heart biopsy: 2177


title: heart biopsy: 2172


title: heart biopsy: 2170


title: heart biopsy: 2169


title: heart biopsy: 2167


title: heart biopsy: 2164


title: heart biopsy: 2165


title: heart biopsy: 2166


title: heart biopsy: 2185


title: heart biopsy: 2161


title: heart biopsy: 2163


title: heart biopsy: 2162


title: heart biopsy: 2160


title: heart biopsy: 2159


title: heart biopsy: 2156


title: heart biopsy: 2157


title: heart biopsy: 2155


title: heart biopsy: 2175


title: heart biopsy: 2158


title: heart biopsy: 2149


title: heart biopsy: 2152


title: heart biopsy: 2147


title: heart biopsy: 2150


title: heart biopsy: 2151


title: heart biopsy: 2148


title: heart biopsy: 2168


title: heart biopsy: 2146


title: heart biopsy: 2138


title: heart biopsy: 2145


title: heart biopsy: 2141


title: heart biopsy: 2133


title: heart biopsy: 2132


title: heart biopsy: 2131


title: heart biopsy: 2129


title: heart biopsy: 2128


title: heart biopsy: 2127


title: heart biopsy: 2125


title: heart biopsy: 2154


title: heart biopsy: 2124


title: heart biopsy: 2153


title: heart biopsy: 2119


title: heart biopsy: 2118


title: heart biopsy: 2142


title: heart biopsy: 2123


title: heart biopsy: 2122


title: heart biopsy: 2139


title: heart biopsy: 2113


title: heart biopsy: 2111


title: heart biopsy: 2120


title: heart biopsy: 2115


title: heart biopsy: 2109


title: heart biopsy: 2126


title: heart biopsy: 2114


title: heart biopsy: 2112


title: heart biopsy: 2117


title: heart biopsy: 2108


title: heart biopsy: 2106


title: heart biopsy: 2105


title: heart biopsy: 2107


title: heart biopsy: 2130


title: heart biopsy: 2104


title: heart biopsy: 2103


title: heart biopsy: 2101


title: heart biopsy: 2100


title: heart biopsy: 2098


title: heart biopsy: 2099


title: heart biopsy: 2097


title: heart biopsy: 2121


title: heart biopsy: 2096


title: heart biopsy: 2095


title: heart biopsy: 2116


title: heart biopsy: 2110


title: heart biopsy: 2089


title: heart biopsy: 2090


title: heart biopsy: 2102


title: heart biopsy: 2092


title: heart biopsy: 2088


title: heart biopsy: 2091


title: heart biopsy: 2086


title: heart biopsy: 2083


title: heart biopsy: 2084


title: heart biopsy: 2085


title: heart biopsy: 2093


title: heart biopsy: 2081


title: heart biopsy: 2078


title: heart biopsy: 2077


title: heart biopsy: 2076


title: heart biopsy: 2075


title: heart biopsy: 2074


title: heart biopsy: 2068


title: heart biopsy: 2094


title: heart biopsy: 2069


title: heart biopsy: 2073


title: heart biopsy: 2067


title: heart biopsy: 2087


title: heart biopsy: 2066


title: heart biopsy: 2064


title: heart biopsy: 2061


title: heart biopsy: 2065


title: heart biopsy: 2058


title: heart biopsy: 2082


title: heart biopsy: 2060


title: heart biopsy: 2062


title: heart biopsy: 2059


title: heart biopsy: 2054


title: heart biopsy: 2063


title: heart biopsy: 2057


title: heart biopsy: 2079


title: heart biopsy: 2055


title: heart biopsy: 2056


title: heart biopsy: 2051


title: heart biopsy: 2053


title: heart biopsy: 2052


title: heart biopsy: 2050


title: heart biopsy: 2071


title: heart biopsy: 2072


title: heart biopsy: 2049


title: heart biopsy: 2047


title: heart biopsy: 2045


title: heart biopsy: 2070


title: heart biopsy: 2048


title: heart biopsy: 2046


title: heart biopsy: 2044


title: heart biopsy: 2080


title: heart biopsy: 2042


title: heart biopsy: 2043


title: heart biopsy: 2036


title: heart biopsy: 2041


title: heart biopsy: 2035


title: heart biopsy: 2038


title: heart biopsy: 2039


title: heart biopsy: 2037


title: heart biopsy: 2030


title: heart biopsy: 2031


title: heart biopsy: 2028


title: heart biopsy: 2026


title: heart biopsy: 2024


title: heart biopsy: 2033


title: heart biopsy: 2034


title: heart biopsy: 2032


title: heart biopsy: 2025


title: heart biopsy: 2027


title: heart biopsy: 2021


title: heart biopsy: 2023


title: heart biopsy: 2020


title: heart biopsy: 2018


title: heart biopsy: 2017


title: heart biopsy: 2019


title: heart biopsy: 2016


title: heart biopsy: 2015


title: heart biopsy: 2013


title: heart biopsy: 2012


title: heart biopsy: 2040


title: heart biopsy: 2009


title: heart biopsy: 2006


title: heart biopsy: 2007


title: heart biopsy: 2011


title: heart biopsy: 2005


title: heart biopsy: 2004


title: heart biopsy: 2001


title: heart biopsy: 1999


title: heart biopsy: 1995


title: heart biopsy: 2003


title: heart biopsy: 1997


title: heart biopsy: 2002


title: heart biopsy: 1996


title: heart biopsy: 2000


title: heart biopsy: 1991


title: heart biopsy: 1993


title: heart biopsy: 1990


title: heart biopsy: 1988


title: heart biopsy: 1989


title: heart biopsy: 1986


title: heart biopsy: 2014


title: heart biopsy: 1984


title: heart biopsy: 1983


title: heart biopsy: 1981


title: heart biopsy: 1994


title: heart biopsy: 2008


title: heart biopsy: 1979


title: heart biopsy: 1980


title: heart biopsy: 1985


title: heart biopsy: 1982


title: heart biopsy: 1998


title: heart biopsy: 1978


title: heart biopsy: 1977


title: heart biopsy: 1975


title: heart biopsy: 1992


title: heart biopsy: 2010


title: heart biopsy: 1974


title: heart biopsy: 1987


title: heart biopsy: 1971


title: heart biopsy: 1969


title: heart biopsy: 1972


title: heart biopsy: 1973


title: heart biopsy: 1968


title: heart biopsy: 1964


title: heart biopsy: 1963


title: heart biopsy: 1966


title: heart biopsy: 1965


title: heart biopsy: 1967


title: heart biopsy: 1962


title: heart biopsy: 1961


title: heart biopsy: 1960


title: heart biopsy: 1958


title: heart biopsy: 1976


title: heart biopsy: 1957


title: heart biopsy: 1959


title: heart biopsy: 1954


title: heart biopsy: 1955


title: heart biopsy: 1951


title: heart biopsy: 1952


title: heart biopsy: 1956


title: heart biopsy: 1950


title: heart biopsy: 1953


title: heart biopsy: 1970


title: heart biopsy: 1946


title: heart biopsy: 1948


title: heart biopsy: 1949


title: heart biopsy: 1947


title: heart biopsy: 1945


title: heart biopsy: 1944


title: heart biopsy: 1943


title: heart biopsy: 1942


title: heart biopsy: 1941


title: heart biopsy: 1939


title: heart biopsy: 1938


title: heart biopsy: 1935


title: heart biopsy: 1936


title: heart biopsy: 1937


title: heart biopsy: 1934


title: heart biopsy: 1940


title: heart biopsy: 1933


title: heart biopsy: 1932


title: heart biopsy: 1931


title: heart biopsy: 1929


title: heart biopsy: 1930


title: heart biopsy: 1926


title: heart biopsy: 1927


title: heart biopsy: 1923


title: heart biopsy: 1924


title: heart biopsy: 1919


title: heart biopsy: 1925


title: heart biopsy: 1920


title: heart biopsy: 1912


title: heart biopsy: 1915


title: heart biopsy: 1922


title: heart biopsy: 1918


title: heart biopsy: 1916


title: heart biopsy: 1917


title: heart biopsy: 1914


title: heart biopsy: 1911


title: heart biopsy: 1910


title: heart biopsy: 1909


title: heart biopsy: 1904


title: heart biopsy: 1906


title: heart biopsy: 1903


title: heart biopsy: 1908


title: heart biopsy: 1905


title: heart biopsy: 1907


title: heart biopsy: 1901


title: heart biopsy: 1928


title: heart biopsy: 1902


title: heart biopsy: 1900


title: heart biopsy: 1899


title: heart biopsy: 1897


title: heart biopsy: 1913


title: heart biopsy: 1896


title: heart biopsy: 1895


title: heart biopsy: 1898


title: heart biopsy: 1893


title: heart biopsy: 1921


title: heart biopsy: 1892


title: heart biopsy: 1894


title: heart biopsy: 1890


title: heart biopsy: 1887


title: heart biopsy: 1889


title: heart biopsy: 1884


title: heart biopsy: 1886


title: heart biopsy: 1885


title: heart biopsy: 1888


title: heart biopsy: 1883


title: heart biopsy: 1881


title: heart biopsy: 1882


title: heart biopsy: 1880


title: heart biopsy: 1878


title: heart biopsy: 1876


title: heart biopsy: 1877


title: heart biopsy: 1874


title: heart biopsy: 1873


title: heart biopsy: 1872


title: heart biopsy: 1871


title: heart biopsy: 1869


title: heart biopsy: 1870


title: heart biopsy: 1891


title: heart biopsy: 1866


title: heart biopsy: 1867


title: heart biopsy: 1865


title: heart biopsy: 1868


title: heart biopsy: 1863


title: heart biopsy: 1860


title: heart biopsy: 1864


title: heart biopsy: 1861


title: heart biopsy: 1859


title: heart biopsy: 1855


title: heart biopsy: 1856


title: heart biopsy: 1858


title: heart biopsy: 1854


title: heart biopsy: 1853


title: heart biopsy: 1850


title: heart biopsy: 1852


title: heart biopsy: 1875


title: heart biopsy: 1879


title: heart biopsy: 1849


title: heart biopsy: 1847


title: heart biopsy: 1851


title: heart biopsy: 1848


title: heart biopsy: 1862


title: heart biopsy: 1845


title: heart biopsy: 1843


title: heart biopsy: 1846


title: heart biopsy: 1842


title: heart biopsy: 1841


title: heart biopsy: 1840


title: heart biopsy: 1839


title: heart biopsy: 1844


title: heart biopsy: 1836


title: heart biopsy: 1857


title: heart biopsy: 1835


title: heart biopsy: 1837


title: heart biopsy: 1834


title: heart biopsy: 1833


title: heart biopsy: 1831


title: heart biopsy: 1829


title: heart biopsy: 1827


title: heart biopsy: 1832


title: heart biopsy: 1826


title: heart biopsy: 1824


title: heart biopsy: 1823


title: heart biopsy: 1821


title: heart biopsy: 1819


title: heart biopsy: 1817


title: heart biopsy: 1816


title: heart biopsy: 1818


title: heart biopsy: 1820


title: heart biopsy: 1838


title: heart biopsy: 1814


title: heart biopsy: 1815


title: heart biopsy: 1813


title: heart biopsy: 1811


title: heart biopsy: 1810


title: heart biopsy: 1830


title: heart biopsy: 1809


title: heart biopsy: 1807


title: heart biopsy: 1806


title: heart biopsy: 1808


title: heart biopsy: 1804


title: heart biopsy: 1803


title: heart biopsy: 1828


title: heart biopsy: 1805


title: heart biopsy: 1802


title: heart biopsy: 1822


title: heart biopsy: 1801


title: heart biopsy: 1825


title: heart biopsy: 1799


title: heart biopsy: 1800


title: heart biopsy: 1797


title: heart biopsy: 1798


title: heart biopsy: 1796


title: heart biopsy: 1795


title: heart biopsy: 1793


title: heart biopsy: 1812


title: heart biopsy: 1790


title: heart biopsy: 1794


title: heart biopsy: 1791


title: heart biopsy: 1787


title: heart biopsy: 1788


title: heart biopsy: 1786


title: heart biopsy: 1784


title: heart biopsy: 1783


title: heart biopsy: 1785


title: heart biopsy: 1780


title: heart biopsy: 1782


title: heart biopsy: 1777


title: heart biopsy: 1774


title: heart biopsy: 1773


title: heart biopsy: 1775


title: heart biopsy: 1778


title: heart biopsy: 1776


title: heart biopsy: 1779


title: heart biopsy: 1771


title: heart biopsy: 1770


title: heart biopsy: 1772


title: heart biopsy: 1767


title: heart biopsy: 1769


title: heart biopsy: 1792


title: heart biopsy: 1789


title: heart biopsy: 1768


title: heart biopsy: 1766


title: heart biopsy: 1765


title: heart biopsy: 1764


title: heart biopsy: 1763


title: heart biopsy: 1762


title: heart biopsy: 1760


title: heart biopsy: 1759


title: heart biopsy: 1758


title: heart biopsy: 1757


title: heart biopsy: 1756


title: heart biopsy: 1754


title: heart biopsy: 1755


title: heart biopsy: 1753


title: heart biopsy: 1752


title: heart biopsy: 1751


title: heart biopsy: 1746


title: heart biopsy: 1747


title: heart biopsy: 1781


title: heart biopsy: 1748


title: heart biopsy: 1743


title: heart biopsy: 1741


title: heart biopsy: 1742


title: heart biopsy: 1738


title: heart biopsy: 1740


title: heart biopsy: 1736


title: heart biopsy: 1739


title: heart biopsy: 1737


title: heart biopsy: 1761


title: heart biopsy: 1734


title: heart biopsy: 1733


title: heart biopsy: 1735


title: heart biopsy: 1731


title: heart biopsy: 1732


title: heart biopsy: 1728


title: heart biopsy: 1730


title: heart biopsy: 1727


title: heart biopsy: 1729


title: heart biopsy: 1726


title: heart biopsy: 1750


title: heart biopsy: 1724


title: heart biopsy: 1749


title: heart biopsy: 1745


title: heart biopsy: 1744


title: heart biopsy: 1721


title: heart biopsy: 1720


title: heart biopsy: 1723


title: heart biopsy: 1719


title: heart biopsy: 1717


title: heart biopsy: 1718


title: heart biopsy: 1716


title: heart biopsy: 1715


title: heart biopsy: 1713


title: heart biopsy: 1714


title: heart biopsy: 1711


title: heart biopsy: 1710


title: heart biopsy: 1712


title: heart biopsy: 1707


title: heart biopsy: 1709


title: heart biopsy: 1703


title: heart biopsy: 1704


title: heart biopsy: 1702


title: heart biopsy: 1725


title: heart biopsy: 1708


title: heart biopsy: 1722


title: heart biopsy: 1699


title: heart biopsy: 1700


title: heart biopsy: 1705


title: heart biopsy: 1693


title: heart biopsy: 1701


title: heart biopsy: 1692


title: heart biopsy: 1690


title: heart biopsy: 1689


title: heart biopsy: 1687


title: heart biopsy: 1697


title: heart biopsy: 1698


title: heart biopsy: 1695


title: heart biopsy: 1696


title: heart biopsy: 1694


title: heart biopsy: 1688


title: heart biopsy: 1683


title: heart biopsy: 1691


title: heart biopsy: 1686


title: heart biopsy: 1706


title: heart biopsy: 1685


title: heart biopsy: 1676


title: heart biopsy: 1674


title: heart biopsy: 1681


title: heart biopsy: 1682


title: heart biopsy: 1678


title: heart biopsy: 1680


title: heart biopsy: 1672


title: heart biopsy: 1673


title: heart biopsy: 1670


title: heart biopsy: 1671


title: heart biopsy: 1677


title: heart biopsy: 1669


title: heart biopsy: 1668


title: heart biopsy: 1667


title: heart biopsy: 1666


title: heart biopsy: 1665


title: heart biopsy: 1663


title: heart biopsy: 1684


title: heart biopsy: 1664


title: heart biopsy: 1661


title: heart biopsy: 1656


title: heart biopsy: 1660


title: heart biopsy: 1654


title: heart biopsy: 1662


title: heart biopsy: 1659


title: heart biopsy: 1653


title: heart biopsy: 1655


title: heart biopsy: 1658


title: heart biopsy: 1675


title: heart biopsy: 1679


title: heart biopsy: 1652


title: heart biopsy: 1650


title: heart biopsy: 1651


title: heart biopsy: 1648


title: heart biopsy: 1646


title: heart biopsy: 1645


title: heart biopsy: 1640


title: heart biopsy: 1639


title: heart biopsy: 1644


title: heart biopsy: 1643


title: heart biopsy: 1637


title: heart biopsy: 1642


title: heart biopsy: 1634


title: heart biopsy: 1641


title: heart biopsy: 1638


title: heart biopsy: 1635


title: heart biopsy: 1636


title: heart biopsy: 1632


title: heart biopsy: 1657


title: heart biopsy: 1631


title: heart biopsy: 1630


title: heart biopsy: 1633


title: heart biopsy: 1649


title: heart biopsy: 1627


title: heart biopsy: 1647


title: heart biopsy: 1624


title: heart biopsy: 1626


title: heart biopsy: 1623


title: heart biopsy: 1628


title: heart biopsy: 1629


title: heart biopsy: 1622


title: heart biopsy: 1621


title: heart biopsy: 1625


title: heart biopsy: 1618


title: heart biopsy: 1620


title: heart biopsy: 1617


title: heart biopsy: 1616


title: heart biopsy: 1615


title: heart biopsy: 1614


title: heart biopsy: 1619


title: heart biopsy: 1612


title: heart biopsy: 1613


title: heart biopsy: 3202


title: heart biopsy: 3203


title: heart biopsy: 1611


title: heart biopsy: 1610


title: heart biopsy: 3201


title: heart biopsy: 3198


title: heart biopsy: 3197


title: heart biopsy: 3200


title: heart biopsy: 3195


title: heart biopsy: 3194


title: heart biopsy: 3190


title: heart biopsy: 3189


title: heart biopsy: 3199


title: heart biopsy: 3192


title: heart biopsy: 3187


title: heart biopsy: 3186


title: heart biopsy: 3191


title: heart biopsy: 3193


title: heart biopsy: 3184


title: heart biopsy: 3185


title: heart biopsy: 3182


title: heart biopsy: 3183


title: heart biopsy: 3179


title: heart biopsy: 3181


title: heart biopsy: 3180


title: heart biopsy: 3178


title: heart biopsy: 3177


title: heart biopsy: 3176


title: heart biopsy: 3175


title: heart biopsy: 3172


title: heart biopsy: 3196


title: heart biopsy: 3170


title: heart biopsy: 3166


title: heart biopsy: 3173


title: heart biopsy: 3171


title: heart biopsy: 3167


title: heart biopsy: 3174


title: heart biopsy: 3188


title: heart biopsy: 3163


title: heart biopsy: 3162


title: heart biopsy: 3161


title: heart biopsy: 3164


title: heart biopsy: 3169


title: heart biopsy: 3158


title: heart biopsy: 3159


title: heart biopsy: 3160


title: heart biopsy: 3157


title: heart biopsy: 3153


title: heart biopsy: 3156


title: heart biopsy: 3154


title: heart biopsy: 3152


title: heart biopsy: 3165


title: heart biopsy: 3150


title: heart biopsy: 3149


title: heart biopsy: 3148


title: heart biopsy: 3146


title: heart biopsy: 3147


title: heart biopsy: 3168


title: heart biopsy: 3145


title: heart biopsy: 3144


title: heart biopsy: 3143


title: heart biopsy: 3140


title: heart biopsy: 3142


title: heart biopsy: 3138


title: heart biopsy: 3141


title: heart biopsy: 3137


title: heart biopsy: 3135


title: heart biopsy: 3134


title: heart biopsy: 3136


title: heart biopsy: 3132


title: heart biopsy: 3133


title: heart biopsy: 3155


title: heart biopsy: 3131


title: heart biopsy: 3130


title: heart biopsy: 3129


title: heart biopsy: 3127


title: heart biopsy: 3123


title: heart biopsy: 3124


title: heart biopsy: 3118


title: heart biopsy: 3119


title: heart biopsy: 3151


title: heart biopsy: 3125


title: heart biopsy: 3126


title: heart biopsy: 3122


title: heart biopsy: 3121


title: heart biopsy: 3120


title: heart biopsy: 3139


title: heart biopsy: 3116


title: heart biopsy: 3117


title: heart biopsy: 3115


title: heart biopsy: 3113


title: heart biopsy: 3111


title: heart biopsy: 3112


title: heart biopsy: 3110


title: heart biopsy: 3114


title: heart biopsy: 3109


title: heart biopsy: 3108


title: heart biopsy: 3105


title: heart biopsy: 3104


title: heart biopsy: 3128


title: heart biopsy: 3103


title: heart biopsy: 3102


title: heart biopsy: 3101


title: heart biopsy: 3106


title: heart biopsy: 3107


title: heart biopsy: 3098


title: heart biopsy: 3096


title: heart biopsy: 3095


title: heart biopsy: 3089


title: heart biopsy: 3087


title: heart biopsy: 3100


title: heart biopsy: 3097


title: heart biopsy: 3099


title: heart biopsy: 3088


title: heart biopsy: 3085


title: heart biopsy: 3086


title: heart biopsy: 3081


title: heart biopsy: 3084


title: heart biopsy: 3078


title: heart biopsy: 3083


title: heart biopsy: 3077


title: heart biopsy: 3076


title: heart biopsy: 3079


title: heart biopsy: 3075


title: heart biopsy: 3080


title: heart biopsy: 3082


title: heart biopsy: 3072


title: heart biopsy: 3071


title: heart biopsy: 3073


title: heart biopsy: 3091


title: heart biopsy: 3090


title: heart biopsy: 3094


title: heart biopsy: 3093


title: heart biopsy: 3092


title: heart biopsy: 3069


title: heart biopsy: 3066


title: heart biopsy: 3067


title: heart biopsy: 3064


title: heart biopsy: 3070


title: heart biopsy: 3060


title: heart biopsy: 3059


title: heart biopsy: 3061


title: heart biopsy: 3056


title: heart biopsy: 3068


title: heart biopsy: 3057


title: heart biopsy: 3065


title: heart biopsy: 3063


title: heart biopsy: 3048


title: heart biopsy: 3055


title: heart biopsy: 3074


title: heart biopsy: 3058


title: heart biopsy: 3054


title: heart biopsy: 3045


title: heart biopsy: 3053


title: heart biopsy: 3052


title: heart biopsy: 3046


title: heart biopsy: 3044


title: heart biopsy: 3042


title: heart biopsy: 3062


title: heart biopsy: 3043


title: heart biopsy: 3049


title: heart biopsy: 3038


title: heart biopsy: 3050


title: heart biopsy: 3041


title: heart biopsy: 3040


title: heart biopsy: 3036


title: heart biopsy: 3034


title: heart biopsy: 3039


title: heart biopsy: 3037


title: heart biopsy: 3035


title: heart biopsy: 3047


title: heart biopsy: 3032


title: heart biopsy: 3051


title: heart biopsy: 3028


title: heart biopsy: 3031


title: heart biopsy: 3026


title: heart biopsy: 3030


title: heart biopsy: 3024


title: heart biopsy: 3025


title: heart biopsy: 3029


title: heart biopsy: 3022


title: heart biopsy: 3033


title: heart biopsy: 3023


title: heart biopsy: 3020


title: heart biopsy: 3019


title: heart biopsy: 3018


title: heart biopsy: 3027


title: heart biopsy: 3017


title: heart biopsy: 3016


title: heart biopsy: 3015


title: heart biopsy: 3013


title: heart biopsy: 3011


title: heart biopsy: 3006


title: heart biopsy: 3005


title: heart biopsy: 3004


title: heart biopsy: 3008


title: heart biopsy: 3010


title: heart biopsy: 3007


title: heart biopsy: 3014


title: heart biopsy: 3003


title: heart biopsy: 2999


title: heart biopsy: 2998


title: heart biopsy: 2997


title: heart biopsy: 3002


title: heart biopsy: 3000


title: heart biopsy: 3021


title: heart biopsy: 2994


title: heart biopsy: 2992


title: heart biopsy: 2991


title: heart biopsy: 2993


title: heart biopsy: 2988


title: heart biopsy: 3012


title: heart biopsy: 2989


title: heart biopsy: 2987


title: heart biopsy: 3009


title: heart biopsy: 2990


title: heart biopsy: 2986


title: heart biopsy: 2984


title: heart biopsy: 2985


title: heart biopsy: 2981


title: heart biopsy: 2983


title: heart biopsy: 2982


title: heart biopsy: 2996


title: heart biopsy: 2977


title: heart biopsy: 2980


title: heart biopsy: 2972


title: heart biopsy: 2971


title: heart biopsy: 2975


title: heart biopsy: 2974


title: heart biopsy: 2973


title: heart biopsy: 2995


title: heart biopsy: 2970


title: heart biopsy: 3001


title: heart biopsy: 2969


title: heart biopsy: 2966


title: heart biopsy: 2967


title: heart biopsy: 2976


title: heart biopsy: 2968


title: heart biopsy: 2963


title: heart biopsy: 2965


title: heart biopsy: 2962


title: heart biopsy: 2964


title: heart biopsy: 2960


title: heart biopsy: 2961


title: heart biopsy: 2959


title: heart biopsy: 2958


title: heart biopsy: 2979


title: heart biopsy: 2957


title: heart biopsy: 2978


title: heart biopsy: 2956


title: heart biopsy: 2955


title: heart biopsy: 2953


title: heart biopsy: 2954


title: heart biopsy: 2952


title: heart biopsy: 2950


title: heart biopsy: 2948


title: heart biopsy: 2943


title: heart biopsy: 2947


title: heart biopsy: 2944


title: heart biopsy: 2941


title: heart biopsy: 2940


title: heart biopsy: 2946


title: heart biopsy: 2942


title: heart biopsy: 2939


title: heart biopsy: 2938


title: heart biopsy: 2937


title: heart biopsy: 2936


title: heart biopsy: 2935


title: heart biopsy: 2933


title: heart biopsy: 2934


title: heart biopsy: 2932


title: heart biopsy: 2929


title: heart biopsy: 2930


title: heart biopsy: 2928


title: heart biopsy: 2931


title: heart biopsy: 2926


title: heart biopsy: 2949


title: heart biopsy: 2945


title: heart biopsy: 2922


title: heart biopsy: 2925


title: heart biopsy: 2923


title: heart biopsy: 2920


title: heart biopsy: 2927


title: heart biopsy: 2921


title: heart biopsy: 2919


title: heart biopsy: 2918


title: heart biopsy: 2916


title: heart biopsy: 2914


title: heart biopsy: 2913


title: heart biopsy: 2912


title: heart biopsy: 2915


title: heart biopsy: 2911


title: heart biopsy: 2909


title: heart biopsy: 2910


title: heart biopsy: 2907


title: heart biopsy: 2908


title: heart biopsy: 2906


title: heart biopsy: 2905


title: heart biopsy: 2903


title: heart biopsy: 2904


title: heart biopsy: 2901


title: heart biopsy: 2899


title: heart biopsy: 2900


title: heart biopsy: 2897


title: heart biopsy: 2896


title: heart biopsy: 2898


title: heart biopsy: 2924


title: heart biopsy: 2895


title: heart biopsy: 2893


title: heart biopsy: 2894


title: heart biopsy: 2917


title: heart biopsy: 2890


title: heart biopsy: 2891


title: heart biopsy: 2889


title: heart biopsy: 2887


title: heart biopsy: 2885


title: heart biopsy: 2884


title: heart biopsy: 2888


title: heart biopsy: 2886


title: heart biopsy: 2882


title: heart biopsy: 2883


title: heart biopsy: 2881


title: heart biopsy: 2879


title: heart biopsy: 2880


title: heart biopsy: 2902


title: heart biopsy: 2877


title: heart biopsy: 2875


title: heart biopsy: 2878


title: heart biopsy: 2892


title: heart biopsy: 2874


title: heart biopsy: 2868


title: heart biopsy: 2873


title: heart biopsy: 2865


title: heart biopsy: 2872


title: heart biopsy: 2866


title: heart biopsy: 2871


title: heart biopsy: 2862


title: heart biopsy: 2859


title: heart biopsy: 2854


title: heart biopsy: 2855


title: heart biopsy: 2869


title: heart biopsy: 2870


title: heart biopsy: 2851


title: heart biopsy: 2864


title: heart biopsy: 2853


title: heart biopsy: 2850


title: heart biopsy: 2867


title: heart biopsy: 2847


title: heart biopsy: 2845


title: heart biopsy: 2840


title: heart biopsy: 2863


title: heart biopsy: 2860


title: heart biopsy: 2861


title: heart biopsy: 2856


title: heart biopsy: 2857


title: heart biopsy: 2835


title: heart biopsy: 2836


title: heart biopsy: 2876


title: heart biopsy: 2834


title: heart biopsy: 2832


title: heart biopsy: 2828


title: heart biopsy: 2852


title: heart biopsy: 2826


title: heart biopsy: 2849


title: heart biopsy: 2838


title: heart biopsy: 2848


title: heart biopsy: 2846


title: heart biopsy: 2844


title: heart biopsy: 2823


title: heart biopsy: 2822


title: heart biopsy: 2843


title: heart biopsy: 2842


title: heart biopsy: 2829


title: heart biopsy: 2820


title: heart biopsy: 2818


title: heart biopsy: 2819


title: heart biopsy: 2817


title: heart biopsy: 2833


title: heart biopsy: 2839


title: heart biopsy: 2831


title: heart biopsy: 2824


title: heart biopsy: 2841


title: heart biopsy: 2816


title: heart biopsy: 2815


title: heart biopsy: 2810


title: heart biopsy: 2812


title: heart biopsy: 2830


title: heart biopsy: 2821


title: heart biopsy: 2858


title: heart biopsy: 2809


title: heart biopsy: 2808


title: heart biopsy: 2805


title: heart biopsy: 2837


title: heart biopsy: 2806


title: heart biopsy: 2804


title: heart biopsy: 2811


title: heart biopsy: 2825


title: heart biopsy: 2801


title: heart biopsy: 2798


title: heart biopsy: 2814


title: heart biopsy: 2807


title: heart biopsy: 2803


title: heart biopsy: 2802


title: heart biopsy: 2813


title: heart biopsy: 2797


title: heart biopsy: 2795


title: heart biopsy: 2792


title: heart biopsy: 2796


title: heart biopsy: 2793


title: heart biopsy: 2794


title: heart biopsy: 2790


title: heart biopsy: 2791


title: heart biopsy: 2788


title: heart biopsy: 2789


title: heart biopsy: 2827


title: heart biopsy: 2786


title: heart biopsy: 2782


title: heart biopsy: 2780


title: heart biopsy: 2784


title: heart biopsy: 2785


title: heart biopsy: 2783


title: heart biopsy: 2799


title: heart biopsy: 2778


title: heart biopsy: 2800


title: heart biopsy: 2777


title: heart biopsy: 2779


title: heart biopsy: 2775


title: heart biopsy: 2776


title: heart biopsy: 2771


title: heart biopsy: 2772


title: heart biopsy: 2770


title: heart biopsy: 2773


title: heart biopsy: 2787


title: heart biopsy: 2769


title: heart biopsy: 2768


title: heart biopsy: 2765


title: heart biopsy: 2767


title: heart biopsy: 2762


title: heart biopsy: 2781


title: heart biopsy: 2759


title: heart biopsy: 2764


title: heart biopsy: 2760


title: heart biopsy: 2761


title: heart biopsy: 2757


title: heart biopsy: 2758


title: heart biopsy: 2756


title: heart biopsy: 2753


title: heart biopsy: 2754


title: heart biopsy: 2752


title: heart biopsy: 2750


title: heart biopsy: 2751


title: heart biopsy: 2746


title: heart biopsy: 2745


title: heart biopsy: 2744


title: heart biopsy: 2748


title: heart biopsy: 2747


title: heart biopsy: 2774


title: heart biopsy: 2763


title: heart biopsy: 2741


title: heart biopsy: 2740


title: heart biopsy: 2738


title: heart biopsy: 2742


title: heart biopsy: 2743


title: heart biopsy: 2766


title: heart biopsy: 2755


title: heart biopsy: 2737


title: heart biopsy: 2735


title: heart biopsy: 2736


title: heart biopsy: 2732


title: heart biopsy: 2730


title: heart biopsy: 2734


title: heart biopsy: 2727


title: heart biopsy: 2733


title: heart biopsy: 2729


title: heart biopsy: 2726


title: heart biopsy: 2731


title: heart biopsy: 2749


title: heart biopsy: 2723


title: heart biopsy: 2718


title: heart biopsy: 2719


title: heart biopsy: 2725


title: heart biopsy: 2724


title: heart biopsy: 2720


title: heart biopsy: 2717


title: heart biopsy: 2728


title: heart biopsy: 2716


title: heart biopsy: 2714


title: heart biopsy: 2739


title: heart biopsy: 2715


title: heart biopsy: 2712


title: heart biopsy: 2711


title: heart biopsy: 2708


title: heart biopsy: 2710


title: heart biopsy: 2707


title: heart biopsy: 2705


title: heart biopsy: 2713


title: heart biopsy: 2706


title: heart biopsy: 2704


title: heart biopsy: 2721


title: heart biopsy: 2701


title: heart biopsy: 2702


title: heart biopsy: 2722


title: heart biopsy: 2700


title: heart biopsy: 2699


title: heart biopsy: 2694


title: heart biopsy: 2693


title: heart biopsy: 2695


title: heart biopsy: 2691


title: heart biopsy: 2697


title: heart biopsy: 2698


title: heart biopsy: 2696


title: heart biopsy: 2692


title: heart biopsy: 2690


title: heart biopsy: 2709


title: heart biopsy: 2688


title: heart biopsy: 2685


title: heart biopsy: 2689


title: heart biopsy: 2687


title: heart biopsy: 2683


title: heart biopsy: 2686


title: heart biopsy: 2703


title: heart biopsy: 2684


title: heart biopsy: 2681


title: heart biopsy: 2682


title: heart biopsy: 2680


title: heart biopsy: 2674


title: heart biopsy: 2675


title: heart biopsy: 2677


title: heart biopsy: 2676


title: heart biopsy: 2671


title: heart biopsy: 2672


title: heart biopsy: 2673


title: heart biopsy: 2679


title: heart biopsy: 2669


title: heart biopsy: 2668


title: heart biopsy: 2666


title: heart biopsy: 2664


title: heart biopsy: 2665


title: heart biopsy: 2667


title: heart biopsy: 2662


title: heart biopsy: 2663


title: heart biopsy: 2661


title: heart biopsy: 2660


title: heart biopsy: 2658


title: heart biopsy: 2657


title: heart biopsy: 2678


title: heart biopsy: 2655


title: heart biopsy: 2654


title: heart biopsy: 2652


title: heart biopsy: 2651


title: heart biopsy: 2653


title: heart biopsy: 2648


title: heart biopsy: 2670


title: heart biopsy: 2656


title: heart biopsy: 2647


title: heart biopsy: 2643


title: heart biopsy: 2646


title: heart biopsy: 2644


title: heart biopsy: 2639


title: heart biopsy: 2642


title: heart biopsy: 2641


title: heart biopsy: 2640


title: heart biopsy: 2638


title: heart biopsy: 2635


title: heart biopsy: 2637


title: heart biopsy: 2659


title: heart biopsy: 2632


title: heart biopsy: 2636


title: heart biopsy: 2634


title: heart biopsy: 2633


title: heart biopsy: 2629


title: heart biopsy: 2630


title: heart biopsy: 2631


title: heart biopsy: 2626


title: heart biopsy: 2650


title: heart biopsy: 2645


title: heart biopsy: 2649


title: heart biopsy: 2623


title: heart biopsy: 2625


title: heart biopsy: 2627


title: heart biopsy: 2621


title: heart biopsy: 2619


title: heart biopsy: 2618


title: heart biopsy: 2620


title: heart biopsy: 2617


title: heart biopsy: 2622


title: heart biopsy: 2616


title: heart biopsy: 2615


title: heart biopsy: 2614


title: heart biopsy: 2611


title: heart biopsy: 2612


title: heart biopsy: 2610


title: heart biopsy: 2609


title: heart biopsy: 2613


title: heart biopsy: 2628


title: heart biopsy: 2607


title: heart biopsy: 2605


title: heart biopsy: 2604


title: heart biopsy: 2603


title: heart biopsy: 2602


title: heart biopsy: 2624


title: heart biopsy: 2597


title: heart biopsy: 2606


title: heart biopsy: 2601


title: heart biopsy: 2595


title: heart biopsy: 2599


title: heart biopsy: 2600


title: heart biopsy: 2592


title: heart biopsy: 2591


title: heart biopsy: 2593


title: heart biopsy: 2598


title: heart biopsy: 2590


title: heart biopsy: 2594


title: heart biopsy: 2596


title: heart biopsy: 2588


title: heart biopsy: 2608


title: heart biopsy: 2585


title: heart biopsy: 2580


title: heart biopsy: 2579


title: heart biopsy: 2586


title: heart biopsy: 2584


title: heart biopsy: 2583


title: heart biopsy: 2582


title: heart biopsy: 2587


title: heart biopsy: 2577


title: heart biopsy: 2581


title: heart biopsy: 2578


title: heart biopsy: 2576


title: heart biopsy: 2589


title: heart biopsy: 2574


title: heart biopsy: 2572


title: heart biopsy: 2563


title: heart biopsy: 2562


title: heart biopsy: 2566


title: heart biopsy: 2571


title: heart biopsy: 2570


title: heart biopsy: 2569


title: heart biopsy: 2568


title: heart biopsy: 2567


title: heart biopsy: 2564


title: heart biopsy: 2558


title: heart biopsy: 2557


title: heart biopsy: 2561


title: heart biopsy: 2560


title: heart biopsy: 2525


title: heart biopsy: 2524


title: heart biopsy: 2573


title: heart biopsy: 2575


title: heart biopsy: 2526


title: heart biopsy: 2559


title: heart biopsy: 2556


title: heart biopsy: 2555


title: heart biopsy: 2516


title: heart biopsy: 2522


title: heart biopsy: 2515


title: heart biopsy: 2518


title: heart biopsy: 2565


title: heart biopsy: 2514


title: heart biopsy: 2505


title: heart biopsy: 2510


title: heart biopsy: 2504


title: heart biopsy: 2554


title: heart biopsy: 2500


title: heart biopsy: 2509


title: heart biopsy: 2502


title: heart biopsy: 2508


title: heart biopsy: 2499


title: heart biopsy: 2498


title: heart biopsy: 2497


title: heart biopsy: 2492


title: heart biopsy: 2506


title: heart biopsy: 2519


title: heart biopsy: 2501


title: heart biopsy: 2488


title: heart biopsy: 2486


title: heart biopsy: 2496


title: heart biopsy: 2491


title: heart biopsy: 2483


title: heart biopsy: 2436


title: heart biopsy: 2487


title: heart biopsy: 2437


title: heart biopsy: 2484


title: heart biopsy: 2507


title: heart biopsy: 656


title: heart biopsy: 2438


title: heart biopsy: 655


title: heart biopsy: 652


title: heart biopsy: 651


title: heart biopsy: 654


title: heart biopsy: 650


title: heart biopsy: 653


title: heart biopsy: 649


title: heart biopsy: 648


title: heart biopsy: 644


title: heart biopsy: 646


title: heart biopsy: 642


title: heart biopsy: 640


title: heart biopsy: 638


title: heart biopsy: 639


title: heart biopsy: 643


title: heart biopsy: 637


title: heart biopsy: 641


title: heart biopsy: 636


title: heart biopsy: 632


title: heart biopsy: 630


title: heart biopsy: 629


title: heart biopsy: 627


title: heart biopsy: 634


title: heart biopsy: 626


title: heart biopsy: 631


title: heart biopsy: 633


title: heart biopsy: 628


title: heart biopsy: 621


title: heart biopsy: 622


title: heart biopsy: 625


title: heart biopsy: 647


title: heart biopsy: 624


title: heart biopsy: 620


title: heart biopsy: 619


title: heart biopsy: 645


title: heart biopsy: 618


title: heart biopsy: 616


title: heart biopsy: 635


title: heart biopsy: 614


title: heart biopsy: 615


title: heart biopsy: 611


title: heart biopsy: 612


title: heart biopsy: 609


title: heart biopsy: 610


title: heart biopsy: 608


title: heart biopsy: 613


title: heart biopsy: 607


title: heart biopsy: 604


title: heart biopsy: 606


title: heart biopsy: 600


title: heart biopsy: 603


title: heart biopsy: 601


title: heart biopsy: 605


title: heart biopsy: 623


title: heart biopsy: 598


title: heart biopsy: 596


title: heart biopsy: 595


title: heart biopsy: 597


title: heart biopsy: 594


title: heart biopsy: 599


title: heart biopsy: 592


title: heart biopsy: 593


title: heart biopsy: 591


title: heart biopsy: 617


title: heart biopsy: 590


title: heart biopsy: 589


title: heart biopsy: 588


title: heart biopsy: 585


title: heart biopsy: 586


title: heart biopsy: 602


title: heart biopsy: 583


title: heart biopsy: 580


title: heart biopsy: 579


title: heart biopsy: 584


title: heart biopsy: 578


title: heart biopsy: 581


title: heart biopsy: 577


title: heart biopsy: 582


title: heart biopsy: 574


title: heart biopsy: 573


title: heart biopsy: 570


title: heart biopsy: 571


title: heart biopsy: 572


title: heart biopsy: 567


title: heart biopsy: 569


title: heart biopsy: 565


title: heart biopsy: 563


title: heart biopsy: 566


title: heart biopsy: 561


title: heart biopsy: 562


title: heart biopsy: 568


title: heart biopsy: 560


title: heart biopsy: 564


title: heart biopsy: 587


title: heart biopsy: 556


title: heart biopsy: 558


title: heart biopsy: 555


title: heart biopsy: 576


title: heart biopsy: 554


title: heart biopsy: 559


title: heart biopsy: 552


title: heart biopsy: 553


title: heart biopsy: 551


title: heart biopsy: 548


title: heart biopsy: 546


title: heart biopsy: 547


title: heart biopsy: 545


title: heart biopsy: 575


title: heart biopsy: 543


title: heart biopsy: 542


title: heart biopsy: 539


title: heart biopsy: 540


title: heart biopsy: 541


title: heart biopsy: 544


title: heart biopsy: 537


title: heart biopsy: 538


title: heart biopsy: 536


title: heart biopsy: 534


title: heart biopsy: 533


title: heart biopsy: 532


title: heart biopsy: 535


title: heart biopsy: 531


title: heart biopsy: 557


title: heart biopsy: 530


title: heart biopsy: 550


title: heart biopsy: 528


title: heart biopsy: 549


title: heart biopsy: 529


title: heart biopsy: 526


title: heart biopsy: 521


title: heart biopsy: 523


title: heart biopsy: 524


title: heart biopsy: 522


title: heart biopsy: 520


title: heart biopsy: 518


title: heart biopsy: 517


title: heart biopsy: 514


title: heart biopsy: 515


title: heart biopsy: 513


title: heart biopsy: 512


title: heart biopsy: 511


title: heart biopsy: 510


title: heart biopsy: 509


title: heart biopsy: 508


title: heart biopsy: 507


title: heart biopsy: 506


title: heart biopsy: 527


title: heart biopsy: 504


title: heart biopsy: 505


title: heart biopsy: 502


title: heart biopsy: 503


title: heart biopsy: 500


title: heart biopsy: 501


title: heart biopsy: 497


title: heart biopsy: 498


title: heart biopsy: 499


title: heart biopsy: 519


title: heart biopsy: 495


title: heart biopsy: 496


title: In vitro activated Naive CD4 T cells, mice 4 FAC

type: SRA

city: Paris

first: Bruno

last: Martin

state: Île-de-France

address: 22, rue Mechain

country: France

institute: Institut Cochin, INSERM U1016, CNRS UMR 8104

description: NormalizedMatrix.tsv

library_source: transcriptomic

data_processing:
Primary analysis based on AOZAN software (ENS, Paris) was applied to demultiplex and control the quality of the raw data (based of FastQC modules / version 0.11.5).
Fastq files were then aligned using STAR algorithm (version 2.7.6a), on the Ensembl release 109 Mus_musculus_GRCm39 reference.
Reads were then count using RSEM (v1.3.1) and the statistical analyses on the read counts were performed with R (version 3.6.3) and the DESeq2 package (DESeq2_1.26.0) to determine the proportion of differentially expressed genes between two conditions.
We used the standard DESeq2 normalization method (DESeq2’s median of ratios with the DESeq function), with a pre-filter of reads and genes (reads uniquely mapped on the genome, or up to 10 different loci with a count adjustement, and genes with at least 10 reads in at least 3 different samples).
Following the package recommendations, we used the Wald test with the contrast function and the Benjamini-Hochberg FDR control procedure to identify the differentially expressed genes.
Assembly: 109 Mus_musculus_GRCm39
Supplementary files format and content: tab-delimited text file includes raw counts for each sample

organism: Mus musculus

source_name: Naive CD4 T cells

growth_protocol: Facs-sorted naive CD4 T cells from peripheral lymph nodes of C57BL/6 mice were cultured in RPMI GlutaMAX supplemented with 10% FCS.

extract_protocol:
Total RNA was extracted using the RNeasy Mini kit (QIAGEN). 250 ng of high quality total RNA was used for the construction of sequencing libraries.
RNA libraries for RNA-seq were prepared using Stranded mRNA Prep kit (Illumina) according to manufacturer instructions.

treatment_protocol: Cells were then activated with coated anti-CD3 (4μg/mL) and anti-CD28 (4μg/mL) antibodies for 20 hours in the presence or absence (Ctrl.) of iron supplementation to cell-culture (FAC).

tag: cell type

value: Naive CD4 T cells

tag: genotype

value: wt

tag: treatment

value: activated for 20 hours with coated anti-CD3 and anti-CD28 antibodies + FAC


title: heart biopsy: 494


title: In vitro activated Naive CD4 T cells, mice 3 FAC

description: NormalizedMatrix.tsv


title: heart biopsy: 493


title: In vitro activated Naive CD4 T cells, mice 3 Ctrl.

description: NormalizedMatrix.tsv

value: activated for 20 hours with coated anti-CD3 and anti-CD28 antibodies


title: heart biopsy: 516


title: heart biopsy: 491


title: In vitro activated Naive CD4 T cells, mice 2 Ctrl.

description: NormalizedMatrix.tsv


title: In vitro activated Naive CD4 T cells, mice 2 FAC

description: NormalizedMatrix.tsv


title: heart biopsy: 492


title: heart biopsy: 490


title: heart biopsy: 525


title: In vitro activated Naive CD4 T cells, mice 1 FAC

description: NormalizedMatrix.tsv


title: In vitro activated Naive CD4 T cells, mice 1 Ctrl.

description: NormalizedMatrix.tsv


title: heart biopsy: 488


title: Single cell multiome 10x from lineage-Sca1+ckit+ sorted marrow WT cells replicate 2 ATAC

city: Seattle

first: Brian

last: Freie

state: WA

address: 1100 Fairview Ave N

department: Basic Sciences

country: USA

institute: Fred Hutchinson Cancer Research Center

library_source: genomic

data_processing:
For single cell multiome, alignment was carried out using Cellranger-Arc from 10X genomics.
Data was analyzed using Seurat and Signac R packages. High quality cells were filtered for RNA (1,000 < reads < 25, 000) and DNA content (>70,000), and datasets were integrated and clustered to define cell categories.
Assembly: mm10
Supplementary files format and content: For single cell multiome data, gene expression values are provided as a matrix for each cell.
Library strategy: sc-multiome

molecule: genomic DNA

source_name: Lin-Scai+ckit+ marrow Cells

extract_protocol:
Single celll multiome analysis ( scRNA-Seq and scATAC-Seq) was done using nuclei isolated according to the manufacturers specifications (10X Genomics). For this, cells were sorted on a flow cytometer (Lin-Sca1+ckit+), and nuclei were prepared in buffer containing digitonin. Cells were counted, barcoded, and extraction was carried out in the Fred Hutch Cancer Center genomics core as described for the 10X genomics system.
After barcoding and extraction, single cell multiome libraries were constructed in the Fred Hutch Cancer Center genomics core using the 10X Genomics system. RNA-seq libraries were prepared from total RNA using the TruSeq RNA Sample Prep v2 kit. For CUT&RUN, cells were processed on a previously published, automated system at the Fred Hutch Cancer Center that uses KAPA DNA polymerase method for library preparation optimized for recovery of small DNA fragments.

value: Lin-Scai+ckit+ marrow Cells

value: WT


title: heart biopsy: 487


title: heart biopsy: 486


title: Single cell multiome 10x from lineage-Sca1+ckit+ sorted marrow WT cells replicate 1 ATAC


title: Single cell multiome 10x from lineage-Sca1+ckit+ sorted marrow T58A cells replicate 1 ATAC


title: heart biopsy: 485


title: heart biopsy: 489


title: In vitro activated Naive CD4 T cells, mice 4 Ctrl.

description: NormalizedMatrix.tsv


title: Single cell multiome 10x from lineage-Sca1+ckit+ sorted marrow WT cells replicate 2 GEX


title: heart biopsy: 483


title: Single cell multiome 10x from lineage-Sca1+ckit+ sorted marrow T58A cells replicate 2 ATAC


title: Single cell multiome 10x from lineage-Sca1+ckit+ sorted marrow T58A cells replicate 2 GEX


title: Single cell multiome 10x from lineage-Sca1+ckit+ sorted marrow WT cells replicate 1 GEX


title: heart biopsy: 481


title: Single cell multiome 10x from lineage-Sca1+ckit+ sorted marrow T58A cells replicate 1 GEX


title: heart biopsy: 480


title: heart biopsy: 479


title: Sample10_RF_S14

city: Aviano

first: Federico

last: Pozzo

state: PN

address: via Franco Gallini 2

department: Department of Translational Research

country: Italy

institute: Centro di Riferimento Oncologico (CRO) di Aviano IRCCS

data_processing:
Custom pipeline
Sequence reads were trimmed with fastp using default settings
Trimmed reads were mapped to hg38 using STAR with default parameters and --quant mode for read count.
Assembly: hg38
Supplementary files format and content: tab-delimited text file includes raw counts for each Sample
Supplementary files format and content: tab-delimited text file includes normalized counts for each Sample

molecule: polyA RNA

source_name: Primary CLL

extract_protocol:
RNA was extracted with Rneasy Micro kit (Qiagen). 30-50ng of total RNA were used as input for library preparation
RNA libraries were prepared using the mRNA library Prep Kit (Illumina) According to manufacturer's protocols

value: Primary CLL

value: RF


title: heart biopsy: 477


title: Sample10_PF_S13

value: PF


title: Sample9_RF_S4


title: Sample8_PF_S5


title: Sample8_RF_S8


title: heart biopsy: 476


title: Sample7_PF_S7


title: heart biopsy: 473


title: heart biopsy: 474


title: Sample7_RF_S8


title: heart biopsy: 484


title: heart biopsy: 471


title: heart biopsy: 482


title: heart biopsy: 472


title: Sample5_PF_S14


title: Sample6_RF_S6


title: Sample5_RF_S15


title: Sample4_PF_S1


title: heart biopsy: 478


title: Sample9_PF_S3


title: Sample3_PF_S9


title: Sample4_RF_S9


title: Sample3_RF_S10


title: heart biopsy: 475


title: Sample2_PF_S1


title: Sample2_RF_S2


title: heart biopsy: 465


title: heart biopsy: 466


title: Sample6_PF_S5


title: heart biopsy: 464


title: heart biopsy: 470


title: heart biopsy: 462


title: heart biopsy: 468


title: heart biopsy: 463


title: heart biopsy: 469


title: heart biopsy: 467


title: Sample1_PF_S4


title: heart biopsy: 461


title: Sample1_RF_S3


title: heart biopsy: 460


title: Primary keratinocytes, Dominant negative RXRa UAB30 treated replicate 4

city: New York

first: Laszlo

last: Halasz

state: NY

address: 1470 Madison Avenue

department: Department of Oncological Sciences

institute: Mount Sinai Icahn School of Medicine

description:
dnRXR_UAB30_Rep4
count_table_raw.txt
count_table_CPM.txt

data_processing:
QuantSeq: Raw reads were processed using the steps in Lexogen's QuantSeq (3' mRNA-Seq) analysis pipeline. Briefly, quality of reads were checked with FastQC, then adapter contamination, polyA read through and low quality tails were removed using BBDuk. Quality filtering passed reads were then aligned to the human genome (GRCh38.p13) using STAR with the following parameters: "--outFilterType BySJout --outFilterMultimapNmax 20 --alignSJoverhangMin 8 --alignSJDBoverhangMin 1 --outFilterMismatchNmax 999 --outFilterMismatchNoverLmax 0.1 --alignIntronMin 20 --alignIntronMax 1000000 --alignMatesGapMax 1000000 --outSAMattributes NH HI NM MD --outSAMtype BAM SortedByCoordinate --quantMode GeneCounts". FeatureCounts was used to quantify gene counts.
Assembly: GRCh38.p13
Supplementary files format and content: RNA-seq: Raw and normalized (CPM) count matrices.

source_name: Primary keratinocytes

growth_protocol: The PHKs were isolated from neonatal foreskins, cultured and infected with retrovirus carrying expression construct for dnRXRα (dnRXRα-raft) or an empty vector (EV) construct encoding only a puromycin resistance marker (EV-raft). Following selection of transduced PHKs for the puromycin resistance encoded by the expression constructs, PHKs were differentiated into organotypic epidermis cultured at the liquid-air interface (“raft” cultures). Stratified cultures were harvested for analyses after 10 days of incubation at the liquid-air interface. For isolation of RNA, epidermis was peeled off the collagen beds and frozen on dry ice.

extract_protocol:
RNA was isolated from skin rafts in Trizol using the Zymo Research Direct-Zol RNA Miniprep kit according to the manufacter's instructions
QuantSeq 3’ mRNA-Seq Library Prep Kit FWD using 100ng of total RNA as input. Libraries constructed according the manufacter's protocol
3' mRNA-seq (QuantSeq)

treatment_protocol: From the day the skin equivalents were lifted onto the grids and until harvest, the raft culture medium was supplemented with either 2 μM UAB30, 0.2 μM UAB110 or vehicle (DMSO) added from 1000x stock. Supplementation with rexinoids was performed under dim light, and the medium was replaced every other day.

value: Skin raft

value: Primary keratinocytes

value: Dominant negative RXRa


title: heart biopsy: 458


title: heart biopsy: 459


title: Primary keratinocytes, Dominant negative RXRa UAB30 treated replicate 2

description:
dnRXR_UAB30_Rep2
count_table_raw.txt
count_table_CPM.txt


title: heart biopsy: 457


title: Primary keratinocytes, Dominant negative RXRa UAB30 treated replicate 1

description:
dnRXR_UAB30_Rep1
count_table_raw.txt
count_table_CPM.txt


title: Primary keratinocytes, Dominant negative RXRa UAB110 treated replicate 4

description:
dnRXR_UAB110_Rep4
count_table_raw.txt
count_table_CPM.txt


title: heart biopsy: 456


title: heart biopsy: 455


title: Primary keratinocytes, Dominant negative RXRa UAB110 treated replicate 3

description:
dnRXR_UAB110_Rep3
count_table_raw.txt
count_table_CPM.txt


title: Primary keratinocytes, Dominant negative RXRa UAB110 treated replicate 2

description:
dnRXR_UAB110_Rep2
count_table_raw.txt
count_table_CPM.txt


title: heart biopsy: 454


title: heart biopsy: 453


title: Primary keratinocytes, Dominant negative RXRa UAB110 treated replicate 1

description:
dnRXR_UAB110_Rep1
count_table_raw.txt
count_table_CPM.txt


title: S. aureus 1 M NaCl biol rep 2

city: Bonn

first: Alina

last: Hoffmann

address: Venusberg Campus 1

country: Germany

institute: University Hospital Bonn

data_processing:
Alignment: HISAT2, version , parameters --no-spliced-alignment --rna-strandness R
Counting: featureCounts, version 1.6.4, parameters -s 2 -t transcript -g locus_tag
Assembly: NCBI Reference Sequence: NZ_CP076660.1
Supplementary files format and content: tab-delimited text file including the normalized hit counts for each Sample, the calculated Log2 fold change and the p-values

organism: Staphylococcus aureus

source_name: Staphylococcus aureus SG511 Berlin

growth_protocol: Cultures of S. aureus SG511 were grown in TSB at 37 °C with aeration until an OD600 of 1.0 was reached.

extract_protocol:
For total RNA extraction, 1 mL culture was harvested (5 min, 8,000  ´ g, RT), the cells were resuspended in 1 mL RNA protect buffer (New England Biolabs, Frankfurt am Main, Germany) and incubated for 5 min at RT. The cells were resuspended in 200 µL TE buffer with 25 µg lysostaphin and cell lysis was achieved by incubation at 37 °C for 30 min. RNA was extracted using the Monarch™ Total RNA Miniprep Kit (New England Biolabs) following the manufacturer’s instructions and stored at −80 °C, before submission to the Genewiz sequencing facility (Leipzig, Germany). RNA samples were quantified using Qubit 4.0 Fluorometer (Life Technologies, Carlsbad, CA, USA) and RNA integrity was checked with RNA Kit on Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA). rRNA depletion was performed using NEBNext rRNA Depletion Kit (Bacteria).
RNA sequencing library preparation was performed using NEBNext Ultra II RNA Library Prep Kit for Illumina by following the manufacturer’s recommendations (NEB, Ipswich, MA, USA).

treatment_protocol: Cultures of S. aureus SG511 were grown in TSB containing 1 M NaCl at 37 °C with aeration until an OD600 of 1.0 was reached.

value: bacterial cells

tag: cell line

value: Staphylococcus aureus SG511 Berlin

tag: growth phase

value: exponential growth phase

value: grown in the presence of 1 M NaCl


title: heart biopsy: 451

value: heart biopsy

value: no


title: heart biopsy: 446


title: heart biopsy: 452


title: heart biopsy: 448


title: S. aureus untreated biol rep 3

value: untreated


title: Primary keratinocytes, Dominant negative RXRa untreated replicate 1

description:
dnRXR_CTR_Rep1
count_table_raw.txt
count_table_CPM.txt


title: heart biopsy: 449


title: Primary keratinocytes, Dominant negative RXRa UAB30 treated replicate 3

description:
dnRXR_UAB30_Rep3
count_table_raw.txt
count_table_CPM.txt


title: S. aureus 1 M NaCl biol rep 3


title: heart biopsy: 447


title: Primary keratinocytes, Dominant negative RXRa untreated replicate 2

description:
dnRXR_CTR_Rep2
count_table_raw.txt
count_table_CPM.txt


title: Primary keratinocytes, Dominant negative RXRa untreated replicate 4

description:
dnRXR_CTR_Rep4
count_table_raw.txt
count_table_CPM.txt


title: heart biopsy: 445


title: heart biopsy: 444


title: Primary keratinocytes, Dominant negative RXRa untreated replicate 3

description:
dnRXR_CTR_Rep3
count_table_raw.txt
count_table_CPM.txt


title: S. aureus untreated biol rep 2


title: Primary keratinocytes, Control UAB30 treated replicate 3

description:
pBabe_UAB30_Rep3
count_table_raw.txt
count_table_CPM.txt

value: Control


title: heart biopsy: 442


title: heart biopsy: 443


title: S. aureus untreated biol rep 1


title: heart biopsy: 440


title: Primary keratinocytes, Control UAB30 treated replicate 4

description:
pBabe_UAB30_Rep4
count_table_raw.txt
count_table_CPM.txt


title: Primary keratinocytes, Control UAB30 treated replicate 2

description:
pBabe_UAB30_Rep2
count_table_raw.txt
count_table_CPM.txt


title: S. aureus 3xMIC 3

data_processing:
Raw sequence data (.bcl files) generated from Illumina NovaSeq was converted into fastq files and de-multiplexed using Illumina bcl2fastq program version 2.20. One mismatch was allowed for index sequence identification.
After investigating the quality of the raw data, sequence reads were trimmed to remove possible adapter sequences and nucleotides with poor quality using Trimmomatic v.0.36.
The trimmed reads were mapped to the reference genome (S. aureus SG511 Berlin; NCBI Reference Sequence: NZ_CP076660.1) using the Bowtie2 aligner v.2.2.6, and BAM files were generated as a result of this step.
Unique gene hit counts were calculated using featureCounts from the Subread package v.1.5.2. Only unique reads that fell within gene regions were counted.
Using DESeq2, a comparison of gene expression between the  groups of samples (untreated versus cervimycin treated) was performed.
The Wald test was used to generate p-values and log2 fold changes. Genes with an adjusted p-value < 0.05 and absolute log2 fold change > 1 were called as differentially expressed genes for each comparison.
Assembly: NCBI Reference Sequence: NZ_CP076660.1
Supplementary files format and content: tab-delimited text file including the normalized hit counts for each Sample, the calculated Log2 fold change and the p-values

source_name: bacterial cells

growth_protocol: Cultures of S. aureus SG511 were grown in TSB at 37 °C with aeration until an OD600 of 0.5 was reached.

treatment_protocol: Exponentially growing S. aureus cells were treated with 3x MIC cervimycin C or the same volume of DMSO for 1 hour.

tag: treatment time

value: 1 hour

value: treated with 3x MIC cervimycin C


title: S. aureus 1 M NaCl biol rep 1


title: Primary keratinocytes, Control UAB30 treated replicate 1

description:
pBabe_UAB30_Rep1
count_table_raw.txt
count_table_CPM.txt


title: heart biopsy: 438


title: heart biopsy: 450


title: heart biopsy: 437


title: heart biopsy: 435


title: S. aureus 3xMIC 2


title: heart biopsy: 436


title: S. aureus untreated 3


title: Primary keratinocytes, Control UAB110 treated replicate 4

description:
pBabe_UAB110_Rep4
count_table_raw.txt
count_table_CPM.txt


title: heart biopsy: 433


title: S. aureus 3xMIC 1


title: heart biopsy: 434


title: Primary keratinocytes, Control UAB110 treated replicate 1

description:
pBabe_UAB110_Rep1
count_table_raw.txt
count_table_CPM.txt


title: Primary keratinocytes, Control UAB110 treated replicate 2

description:
pBabe_UAB110_Rep2
count_table_raw.txt
count_table_CPM.txt


title: heart biopsy: 432


title: S. aureus untreated 1


title: heart biopsy: 427


title: heart biopsy: 430


title: heart biopsy: 441


title: Primary keratinocytes, Control untreated replicate 3

description:
pBabe_CTR_Rep3
count_table_raw.txt
count_table_CPM.txt


title: heart biopsy: 428


title: heart biopsy: 429


title: YAA00198

city: Madrid

first: Osvaldo

last: Graña

state: Comunidad de Madrid

address: C/ Melchor Fernández Almagro, 3

department: Structural Biology

country: Spain

institute: CNIO

data_processing:
Read adapters and polyA tails were removed with bbduk.sh, following the Lexogen recommendations. Processed reads were analysed with the nextpresso pipeline, as follows: Sequencing quality was checked with FastQC v0.11.7 (http://www.bioinformatics.babraham.ac.uk/projects/fastqc/). Reads were aligned to the mouse reference genome (GRCm38) with TopHat-2.0.10 using Bowtie 1.0.0 and Samtools 0.1.19 (--library-type fr-secondstrand in TopHat), allowing three mismatches and twenty multihits. Read counts were obtained with HTSeq-count v0.6.1 (--stranded=yes), using the mouse gene annotation from GENCODE (gencode.vM20.GRCm38.Ensembl95). Differential expression was performed with DESeq2, using a 0.05 FDR.  GSEAPreranked was used to perform gene set enrichment analysis for several gene signatures on a pre-ranked gene list, setting 1000 gene set permutations. Only those gene sets with significant enrichment levels (FDR q-value < 0.25) were considered.
Assembly: GRCm38
Supplementary files format and content: Tabulated text file with DESeq2 normalized counts

source_name: liver

extract_protocol:
Total RNA was extracted from tissues with TRItidy (Panreac Applichem, #A4051,0200) and using RNeasy kit from Qiagen (#74106) according to manufacturer´s instructions
Total RNA samples (500 ng) were used. Sample RNA Integrity numbers were 7.2 on average (range 6.7-7.9) when assayed on an Agilent 2100 Bioanalyzer. Sequencing libraries were prepared with the "QuantSeq 3‘ mRNA-Seq Library Prep Kit (FWD) for Illumina" (Lexogen, Cat.No. 015) by following manufacturer instructions. Library generation is initiated by reverse transcription with oligodT priming, and a second strand synthesis is performed from random primers by a DNA polymerase. Primers from both steps contain Illumina-compatible sequences. Libraries are completed by PCR. This kit generates directional libraries stranded in the sense orientation (the read1 (the only read in single read format) has the sense orientation. cDNA libraries are purified, applied to an Illumina flow cell for cluster generation and sequenced on an Illumina NextSeq 550 (with v2.5 reagent kits) by following manufacturer's protocols.

value: liver

value: whole tissue

value: RagC S74N (KI/+)

tag: age

value: young (2 months old)


title: heart biopsy: 431


title: Primary keratinocytes, Control untreated replicate 4

description:
pBabe_CTR_Rep4
count_table_raw.txt
count_table_CPM.txt

value: Skin raft


title: heart biopsy: 439


title: heart biopsy: 426


title: heart biopsy: 425


title: Primary keratinocytes, Control UAB110 treated replicate 3

description:
pBabe_UAB110_Rep3
count_table_raw.txt
count_table_CPM.txt

value: Primary keratinocytes


title: YAA00190

description: Illumina NextSeq 550 (with v2.5 reagent kits)

value: young (2.5 months old)


title: Primary keratinocytes, Control untreated replicate 2

description:
pBabe_CTR_Rep2
count_table_raw.txt
count_table_CPM.txt


title: heart biopsy: 423


title: heart biopsy: 424


title: S. aureus untreated 2

value: bacterial cells


title: Primary keratinocytes, Control untreated replicate 1

description:
pBabe_CTR_Rep1
count_table_raw.txt
count_table_CPM.txt


title: heart biopsy: 422


title: YAA00189


title: YAA00185

value: young (3 months old)


title: heart biopsy: 420


title: heart biopsy: 419


title: heart biopsy: 421


title: YAA00180


title: heart biopsy: 418


title: heart biopsy: 416


title: YAA00170


title: heart biopsy: 415


title: heart biopsy: 413


title: heart biopsy: 414


title: YAA00160


title: heart biopsy: 417


title: heart biopsy: 412


title: YAA712

data_processing:
UMIs were first extracted with UMItools. Read adapters, polyA tails and rRNA sequences were then removed with bbduk.sh, following the Lexogen recommendations. Reads were aligned with Bowtie2 to the mouse reference genome (GRCm39). SAM files were converted to BAM and sorted with samtools 1.9. Sorted BAM files were deduplicated with UMItools and converted to FASTQ format.  Processed reads were finally analysed with the nextpresso pipeline, as follows: Sequencing quality was checked with FastQC v0.11.7 (http://www.bioinformatics.babraham.ac.uk/projects/fastqc/). Reads were aligned to the mouse reference genome (GRCm39) with TopHat-2.0.10 using Bowtie 1.0.0 (--library-type fr-secondstrand in TopHat), allowing three mismatches and twenty multihits. Read counts were obtained with HTSeq-count v0.6.1 (--stranded=yes), using the mouse gene annotation from GENCODE (gencode.vM26.GRCm39.Ensembl103). Differential expression was performed with DESeq2, using a 0.05 FDR.  GSEAPreranked was used to perform gene set enrichment analysis for several gene signatures on a pre-ranked gene list, setting 1000 gene set permutations. Only those gene sets with significant enrichment levels (FDR q-value < 0.25) were considered.
Assembly: GRCm39
Supplementary files format and content: Tabulated text file with DESeq2 normalized counts

source_name: Bone marrow derived Neutrophils

extract_protocol:
RNA was extracted from cells with TRItidy and Picopure RNA Isolation Kit (Arcturus, Applied Biosystems, #12204-01) following manufacturer´s instructions.
Total RNA samples (average 100ng, range 16-180ng) were processed into cDNA sequencing libraries with the "QuantSeq 3‘ mRNA-Seq Library Prep Kit (FWD) for Illumina" (Lexogen, Cat.No. 015) with a UMI Second Strand Synthesis module. Briefly, library generation is initiated by reverse transcription with oligodT priming, followed by a random-primed second strand synthesis. While primers from both steps contain Illumina-compatible sequences, random primers additionally feature 6 nt long Unique Molecular Identifier (UMI) tags. Libraries are completed by PCR, and sequenced on anIllumina NextSeq 550 (with v2.5 reagent kits) by following manufacturer's protocols. This library preparation kit generates directional libraries stranded in the sense orientation (the read1 (the only read in single read format) has the sense orientation.

value: Bone marrow derived Neutrophils

value: young ( 2 months old)


title: YAA00164


title: heart biopsy: 411

value: heart biopsy


title: heart biopsy: 410

value: no


title: si-OCT4 POVPC treated 3

city: Cleveland

first: Olga

last: Cherepanova

state: Ohio

address: 9620 Carnegie Avenue

department: Cardiovascular and metabolic sciences

institute: Cleveland Clinic Foundation

description: LncRNA/mRNA expression profiling of MASMC treated with siOCT4 for 48h followed by POVPC for 24h, Replicate 3

hyb_protocol: 1 μg of each labeled cRNA was fragmented by adding 5μl 10 × Blocking Agent and 1μlof 25 × Fragmentation Buffer, then heated the mixture at 60 °C for 30 min, finally 25μl 2 × GE Hybridization buffer was added to dilute the labeled cRNA. 50μl of hybridization solution was dispensed into the gasket slide and assembled to the LncRNA expression microarray slide. The slides were incubated for 17 hours at 65°C in an Agilent Hybridization Oven.

scan_protocol: The hybridized arrays were washed, fixed and scanned with using the Agilent DNA Microarray Scanner (part number G2505C).

data_processing: Agilent Feature Extraction software (version 11.0.1.1) was used to analyze acquired array images. Quantile normalization and subsequent data processing were performed using GeneSpring GX v12.1 software package (Agilent Technologies). After quantile normalization of the raw data, LncRNAs and mRNAs that at least 3 out of 12 samples have flags in Present or Marginal (“All Targets Value”) were chosen for further data analysis.

source_name: Primary mouse aortic smooth muscle cells (MASMC), siOCT4 48, POVPC 24h, Replicate 3

label_protocol: Sample labeling and array hybridization were performed according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology) with minor modifications. Briefly, each RNA sample was amplified and transcribed into fluorescent cRNA along the entire length of the transcripts without 3’ bias utilizing a random priming method (Arraystar Flash RNA Labeling Kit, Arraystar). The labeled cRNAs were purified by RNeasy Mini Kit (Qiagen). The concentration and specific activity of the labeled cRNAs (pmol Cy3/μgcRNA) were measured by NanoDrop ND-1000.

extract_protocol: cells were harvested with the Trizol reagent (Invitrogen), and the total RNA was isolated as per the manufacturer-recommended protocol. The total RNA isolated from MASMC was quantified by the Nanodrop ND-1000, and RNA integrity was assessed by standard denaturing agarose gel electrophoresis. For microarray analysis, the Agilent Array platform was employed.

treatment_protocol: Primary mouse aortic smooth muscle cells (MASMC) were isolated and grown as previously described (Cherepanova et al., 2016). For experiments, cells at the 70% confluency were transfected with blocking siOct4 or non-target siRNA (NT siRNA) for 48 hours in serum-free media, followed by the treatment with 10 µg/mL POVPC (Cayman Chem, #10031) or vehicle (DMSO) for 24 hours based on our previous findings (Pidkovka et al., 2007).

value: aortic smooth muscle cells

value: smooth muscle cells


title: heart biopsy: 409


title: YAA711


title: heart biopsy: 408


title: YAA710


title: heart biopsy: 406


title: si-OCT4 POVPC treated 1

description: LncRNA/mRNA expression profiling of MASMC treated with siOCT4 for 48h followed by POVPC for 24h, Replicate 1

source_name: Primary mouse aortic smooth muscle cells (MASMC), siOCT4 48h, POVPC 24h, Replicate 1


title: si-OCT4 POVPC treated 2

description: LncRNA/mRNA expression profiling of MASMC treated with siOCT4 for 48h followed by POVPC for 24h, Replicate 2

source_name: Primary mouse aortic smooth muscle cells (MASMC), siOCT4 48h, POVPC 24h, Replicate 2


title: si-OCT4 DMSO treated 1

description: LncRNA/mRNA expression profiling of MASMC treated with siOCT4 for 48h followed by DMSO for 24h, Replicate 1

source_name: Primary mouse aortic smooth muscle cells (MASMC), siOCT4 48h, DMSO 24h, Replicate 1


title: si-OCT4 DMSO treated 3

description: LncRNA/mRNA expression profiling of MASMC treated with siOCT4 for 48h followed by DMSO for 24h, Replicate 3

source_name: Primary mouse aortic smooth muscle cells (MASMC), siOCT4 48h, DMSO 24h, Replicate 3


title: si-OCT4 DMSO treated 2

description: LncRNA/mRNA expression profiling of MASMC treated with siOCT4 for 48h followed by DMSO for 24h, Replicate 2

source_name: Primary mouse aortic smooth muscle cells (MASMC), siOCT4 48h, DMSO 24h, Replicate 2


title: heart biopsy: 404


title: heart biopsy: 402


title: heart biopsy: 407


title: YAA704


title: YAA703


title: heart biopsy: 403


title: heart biopsy: 401


title: heart biopsy: 405


title: heart biopsy: 399


title: YAA702


title: YAA709


title: heart biopsy: 398