title: Plate14_N17

value: Methyl dodecanoate_0.2_uM


title: Plate14_L07

status: Public on Jul 23 2024

type: SRA

city: College Station

first: Ivan

last: Rusyn

state: TX

address: 4458 TAMU

department: Department of Veterinary Integrative Biosciences

country: USA

institute: Texas A&M University

library_source: transcriptomic

data_processing:
The libraries were sequenced (single-end, 50 bp in length) using HiSeq 2500 v.2 (Illumina, San Diego, CA).
This pipeline includes the use of STAR aligner (Dobin et al., 2013) and parameters used herein included a maximum number of mismatches of 3 and insertion and deletion open and extension penalties of 0.
the following steps were undertaken to filter out low-count genes and samples across the entire dataset: 1) Genes with fewer than 10 mean counts across all samples (n = 4558) were excluded, 2) Samples with an average expression across all genes lower than 15 were also removed. Principal component analysis (PCA) was conducted on vehicle controls from all samples across 16 plates to assess potential plate effects. Outliers in vehicle control samples were identified and excluded using a mean correlation threshold of <0.85, ensuring the reliability of the subsequent transcriptomic dose-response and differential expression analyses.
Assembly: Whole transcriptome assay
Supplementary files format and content: with normalized count data using DESeq2 R package (Love et al., 2014)
Supplementary files format and content: meta data of the QC samples

molecule: total RNA

organism: Homo sapiens

source_name: iPSC-Cardiomyocytes

extract_protocol:
The media and chemicals from the 384-well plates was fully aspirated and replaced with 10 μL/well of 2X lysis buffer. The plates were then shaken using an orbital plate shaker for 10 minutes. The plates were then checked to ensure that the cells were lysed, and if not placed back on the plate shaker for an additional five minutes. Once the cells were lysed, adhesive seals were placed on the plates and the lysates were frozen in the 384-well plates and stored at −80 °C until further processing.  The Templated Oligonucleotide Sequencing Assay (TempO-Seq™, BioSpyder Technologies, Carlsbad, CA) was used as an RNA sequencing technology of choice. Detailed protocols for TempO-seq are provided by the manufacturer and were previously reported  detailed elsewhere (Grimm et al., 2016; House et al., 2022).
TempO-seq libraries were prepared using the human whole transcriptome panel consisting of 22,537 protein-coding transcripts.

tag: cell type

value: iPSC-Cardiomyocytes

tag: treatment

value: Pirinixic acid_10_uM


title: Plate14_L10


title: Plate14_L05


title: Plate14_L12


title: Plate14_L14

value: 2,2'-Bisphenol F_10_uM


title: Plate14_L11


title: Plate14_N18


title: Plate14_L09


title: Plate14_L13


title: Plate14_L17

value: Methyl dodecanoate_10_uM


title: Plate14_L15


title: Plate14_L16


title: Plate14_L08


title: Plate14_N22


title: Plate14_L19


title: Plate14_L21


title: Plate14_L20


title: Plate14_L23


title: Plate14_L22


title: Plate14_M02


title: Plate14_M03


title: Plate14_N19


title: Plate14_M04


title: Plate14_M07

value: Pirinixic acid_1_uM


title: Plate14_J09


title: Plate14_J11


title: Plate14_J12


title: Plate14_J10


title: Plate14_J14


title: Plate14_M09


title: Plate14_L18


title: Plate14_J08


title: Plate14_M10


title: Plate14_J21


title: Plate14_J16


title: Plate14_J17


title: Plate14_J19


title: Plate14_J15


title: Plate14_J20


title: Plate14_J18


title: Plate14_J22

value: Benzyl butyl phthalate_1_uM


title: Plate14_J23


title: Plate14_M05


title: Plate14_K03

value: Citric acid _0.2_uM


title: Plate14_M06


title: Plate14_K02


title: Plate14_K06


title: Plate14_K05


title: Plate14_K04


title: Plate14_K09


title: Plate14_K08


title: Plate14_M08


title: Plate14_K11


title: Plate14_K10


title: Plate14_K12


title: Plate14_K15


title: Plate14_K16


title: Plate14_K14


title: Plate14_K17


title: Plate14_K18


title: Plate14_H19


title: Plate14_K07


title: Plate14_H17


title: Plate14_H21


title: Plate14_K20


title: Plate14_I04


title: Plate14_H23


title: Plate14_I03

value: Citric acid _10_uM


title: Plate14_I02


title: Plate14_H18


title: Plate14_H22

value: Benzyl butyl phthalate_0.2_uM


title: Plate14_H20


title: Plate14_I07


title: Plate14_I10


title: Plate14_I12


title: Plate14_I08


title: Plate14_I06


title: Plate14_I11


title: Plate14_I05


title: Plate14_I14


title: Plate14_I15


title: Plate14_I17


title: Plate14_K19


title: Plate14_I19


title: Plate14_I20


title: Plate14_I22

value: Benzyl butyl phthalate_10_uM


title: Plate14_I23


title: Plate14_J02


title: Plate14_J04


title: Plate14_G07


title: Plate14_J03

value: Citric acid _1_uM


title: Plate14_G04


title: Plate14_J05


title: Plate14_I09


title: Plate14_J06


title: Plate14_J07


title: Plate14_G12


title: Plate14_G14


title: Plate14_G05


title: Plate14_G10


title: Plate14_G06


title: Plate14_G11


title: Plate14_G15


title: Plate14_G09


title: Plate14_I16


title: Plate14_G16


title: Plate14_I21


title: Plate14_G19


title: Plate14_G17


title: Plate14_I18


title: Plate14_G20


title: Plate14_G18


title: Plate14_G21


title: Plate14_G08


title: Plate14_G23


title: Plate14_G22


title: Plate14_H02


title: Plate14_H03


title: Plate14_H04


title: Plate14_H09


title: Plate14_H05


title: Plate14_H14


title: Plate14_H08


title: Plate14_H16


title: Plate14_H07


title: Plate14_E19


title: Plate14_E18


title: Plate14_E20


title: Plate14_H06


title: Plate14_H15


title: Plate14_H10


title: Plate14_E22


title: Plate14_F04


title: Plate14_E21


title: Plate14_F02


title: Plate14_F05


title: Plate14_E16


title: Plate14_F03


title: Plate14_E23


title: Plate14_H11


title: Plate14_F06


title: Plate14_H12


title: Plate14_F07


title: Plate14_E17


title: Plate14_F09


title: Plate14_F08


title: Plate14_F12


title: Plate14_F11


title: Plate14_F10


title: Plate14_F17


title: Plate14_F19


title: Plate14_F20


title: Plate14_F18


title: Plate14_G02


title: Plate14_F15


title: Plate14_F14


title: Plate14_G03


title: Plate14_F16


title: Plate14_D15


title: Plate14_F22


title: Plate14_F21


title: Plate14_D16


title: Plate14_F23


title: Plate14_D19


title: Plate14_D17


title: Plate14_E03


title: Plate14_D18


title: Plate14_D21


title: Plate14_D20


title: Plate14_D23


title: Plate14_E04


title: Plate14_D22


title: Plate14_E02


title: Plate14_E05


title: Plate14_E10


title: Plate14_E09


title: Plate14_E11


title: Plate14_E12


title: Plate14_E06


title: Plate14_E14


title: Plate14_E15


title: Plate14_E08


title: Plate14_E07


title: Plate14_C19


title: Plate14_C18


title: Plate14_C23


title: Plate14_D03


title: Plate14_D02


title: Plate14_C20


title: Plate14_D04


title: Plate14_C21


title: Plate14_C22


title: Plate14_D06


title: Plate14_D14


title: Plate14_D08


title: Plate14_D07


title: Plate14_C03


title: Plate14_D09


title: Plate14_C05


title: Plate14_D10


title: Plate14_D12


title: Plate14_C14


title: Plate14_D05


title: Plate14_C07


title: Plate14_C12


title: Plate14_C08


title: Plate14_C11


title: Plate14_C09


title: Plate14_C17


title: Plate14_B11


title: Plate14_B15


title: Plate14_D11


title: Plate14_B13


title: Plate14_B12


title: Plate14_C10


title: Plate14_C04


title: Plate14_B17


title: Plate14_C15


title: Plate14_B19


title: Plate14_B14


title: Plate14_B16


title: Plate14_B22


title: Plate14_B20


title: Plate14_C06


title: Plate14_B21


title: Plate14_B02


title: Plate14_B23


title: Plate14_C02


title: Plate14_B03


title: Plate14_B05


title: Plate14_B07


title: Plate14_C16


title: Plate14_B08


title: Plate14_B06


title: Plate14_B04


title: Plate14_B18


title: Plate14_B09


title: Plate13_N16


title: Plate13_N19


title: Plate13_N13


title: Plate13_N17


title: Plate13_N20


title: Plate13_N21


title: Plate13_N18


title: Plate13_N14


title: Plate13_L23


title: Plate13_L22


title: Plate13_L19


title: Plate13_N23


title: Plate13_L20


title: Plate13_M03


title: Plate13_M06


title: Plate13_M05


title: Plate13_M04


title: Plate14_B10


title: Plate13_M07


title: Plate13_M10


title: Plate13_M09


title: Plate13_L21


title: Plate13_M12


title: Plate13_N15


title: Plate13_M02


title: Plate13_M11


title: Plate13_M14


title: Plate13_M13


title: Plate13_M15


title: Plate13_N22


title: Plate13_N05


title: Plate13_M20


title: Plate13_M22


title: Plate13_M18


title: Plate13_N04


title: Plate13_N02


title: Plate13_M23


title: Plate13_M17


title: Plate13_M19


title: Plate13_N06


title: Plate13_M08


title: Plate13_N07


title: Plate13_N03


title: Plate13_N08


title: Plate13_N09


title: Plate13_N10


title: Plate13_M16


title: Plate13_N11


title: Plate13_N12


title: Plate13_M21


title: Plate13_K06


title: Plate13_K08


title: Plate13_K07

value: Methyl parathion_0.2_uM


title: Plate13_K11


title: Plate13_K13

value: Darbufelone mesylate_0.2_uM


title: Plate13_K09


title: Plate13_K10

value: Acetaminophen _0.2_uM


title: Plate13_K14


title: Plate13_K15


title: Plate13_K21

value: Raloxifene hydrochloride_0.2_uM


title: Plate13_K22

value: Cyproterone acetate_0.2_uM


title: Plate13_L05


title: Plate13_K23


title: Plate13_L03


title: Plate13_K17


title: Plate13_K16


title: Plate13_L06


title: Plate13_K19


title: Plate13_K18


title: Plate13_K20

value: Allura Red C.I.16035_0.2_uM


title: Plate13_L07

value: iPSC-Cardiomyocytes


title: Plate13_L04


title: Plate13_L02


title: Plate13_L09


title: Plate13_L08


title: Plate13_L10


title: Plate13_K12


title: Plate13_L11


title: Plate13_L16


title: Plate13_L18


title: Plate13_L14


title: Plate13_L13


title: Plate13_I18


title: Plate13_I15


title: Plate13_I16


title: Plate13_I17


title: Plate13_L17


title: Plate13_J06


title: Plate13_J02


title: Plate13_I23


title: Plate13_J03


title: Plate13_J04


title: Plate13_I19


title: Plate13_I21

value: Raloxifene hydrochloride_10_uM


title: Plate13_I20

value: Allura Red C.I.16035_10_uM


title: Plate13_J08


title: Plate13_J05

value: Acetyl tributyl citrate_1_uM


title: Plate13_J09


title: Plate13_L15


title: Plate13_J07

value: Methyl parathion_1_uM


title: Plate13_J10

value: Acetaminophen _1_uM


title: Plate13_J12


title: Plate13_J11


title: Plate13_L12


title: Plate13_J13

value: Darbufelone mesylate_1_uM


title: Plate13_J14


title: Plate13_J15


title: Plate13_J16


title: Plate13_J17


title: Plate13_J21

value: Raloxifene hydrochloride_1_uM


title: Plate13_J19


title: Plate13_J22

value: Cyproterone acetate_1_uM


title: Plate13_I22

value: Cyproterone acetate_10_uM


title: Plate13_J23


title: Plate13_J18


title: Plate13_J20

value: Allura Red C.I.16035_1_uM


title: Plate13_K03


title: Plate13_K05

value: Acetyl tributyl citrate_0.2_uM


title: Plate13_K02


title: Plate13_H08


title: Plate13_H07

value: Methyl parathion_0.2_uM


title: Plate13_H09


title: Plate13_H06


title: Plate13_H10

value: Acetaminophen _0.2_uM


title: Plate13_H12


title: Plate13_H11


title: Plate13_H14


title: Plate13_H15


title: Plate13_H13

value: Darbufelone mesylate_0.2_uM


title: Plate13_H19


title: Plate13_H20


title: Plate13_H17


title: Plate13_H18


title: Plate13_H22

value: Cyproterone acetate_0.2_uM


title: Plate13_H23


title: Plate13_H21

value: Raloxifene hydrochloride_0.2_uM


title: Plate13_I03

value: iPSC-Cardiomyocytes


title: Plate13_I05

value: Acetyl tributyl citrate_10_uM


title: Plate13_I04


title: Plate13_I02


title: Plate13_H05


title: Plate13_K04


title: Plate13_I07

value: Methyl parathion_10_uM


title: Plate13_I10

value: Acetaminophen _10_uM


title: Plate13_I06


title: Plate13_H16


title: Plate13_I09


title: Plate13_I12


title: Plate13_I14


title: Plate13_I13

value: Darbufelone mesylate_10_uM


title: Plate13_F19


title: Plate13_F16


title: Plate13_F20

value: Allura Red C.I.16035_10_uM


title: Plate13_F17


title: Plate13_F18


title: Plate13_F21

value: Raloxifene hydrochloride_10_uM


title: Plate13_F15


title: Plate13_F14


title: Plate13_G02


title: Plate13_F23


title: Plate13_F22


title: Plate13_I08


title: Plate13_G03


title: Plate13_G04


title: Plate13_G07

value: Methyl parathion_1_uM


title: Plate13_G06


title: Plate13_G05

value: Acetyl tributyl citrate_1_uM


title: Plate13_I11


title: Plate13_G08


title: Plate13_G11


title: Plate13_G10

value: Acetaminophen _1_uM


title: Plate13_G14


title: Plate13_G12


title: Plate13_G15


title: Plate13_G18


title: Plate13_G17


title: Plate13_G19


title: Plate13_H04


title: Plate13_E06


title: Plate13_G22

value: Cyproterone acetate_1_uM


title: Plate13_H03


title: Plate13_G20

value: Allura Red C.I.16035_1_uM


title: Plate13_G09


title: Plate13_E07

value: Methyl parathion_0.2_uM


title: Plate13_G21

value: Raloxifene hydrochloride_1_uM


title: Plate13_E09


title: Plate13_G13

value: Darbufelone mesylate_1_uM


title: Plate13_E10

value: Acetaminophen _0.2_uM


title: Plate13_E08


title: Plate13_E12


title: Plate13_E11


title: Plate13_E15


title: Plate13_G16


title: Plate13_E13

value: Darbufelone mesylate_0.2_uM


title: Plate13_E17

value: iPSC-Cardiomyocytes


title: Plate13_E18


title: Plate13_E14


title: Plate13_E16


title: Plate13_E19


title: Plate13_E20

value: Allura Red C.I.16035_0.2_uM


title: Plate13_E21

value: Raloxifene hydrochloride_0.2_uM


title: Plate13_E22

value: Cyproterone acetate_0.2_uM


title: Plate13_E05

value: Acetyl tributyl citrate_0.2_uM


title: Plate13_G23


title: Plate13_H02


title: Plate13_F05

value: Acetyl tributyl citrate_10_uM


title: Plate13_F09


title: Plate13_F10

value: Acetaminophen _10_uM


title: Plate13_F11


title: Plate13_F04


title: Plate13_F07

value: Methyl parathion_10_uM


title: Plate13_F06


title: Plate13_D04


title: Plate13_F13

value: Darbufelone mesylate_10_uM


title: Plate13_F12


title: Plate13_F08


title: Plate13_D05

value: Acetyl tributyl citrate_1_uM


title: Plate13_E23


title: Plate13_D06


title: Plate13_D07

value: Methyl parathion_1_uM


title: Plate13_D10

value: Acetaminophen _1_uM


title: Plate13_D11


title: Plate13_D14


title: Plate13_D08


title: Plate13_D13


title: Plate13_D15


title: Plate13_F02


title: Plate13_D19


title: Plate13_D09


title: Plate13_D18


title: Plate13_D16


title: Plate13_D12


title: Plate13_D17


title: Plate13_D20

value: Allura Red C.I.16035_1_uM


title: Plate13_F03


title: Plate13_E02


title: Plate13_D21

value: Raloxifene hydrochloride_1_uM


title: Plate13_E03


title: Plate13_E04


title: Plate13_D22

value: Cyproterone acetate_1_uM


title: Plate13_C10


title: Plate13_C11


title: Plate13_C12


title: Plate13_C13


title: Plate13_C16


title: Plate13_C15


title: Plate13_C18


title: Plate13_C17


title: Plate13_C14


title: Plate13_C19


title: Plate13_C21

value: Raloxifene hydrochloride_10_uM


title: Plate13_C22

value: Cyproterone acetate_10_uM


title: Plate13_C23

value: iPSC-Cardiomyocytes


title: Plate13_D03


title: Plate13_B16


title: Plate13_B17


title: Plate13_B18


title: Plate13_B20


title: Plate13_C20

value: Allura Red C.I.16035_10_uM


title: Plate13_C09


title: Plate13_D02


title: Plate13_B19


title: Plate13_B22


title: Plate13_B21


title: Plate13_B23


title: Plate13_C02


title: Plate13_C03


title: Plate13_C04


title: Plate13_C05


title: Plate13_C07


title: Plate13_B03


title: Plate13_C06


title: Plate13_B05


title: Plate13_B04


title: Plate13_B02


title: Plate13_B06


title: Plate13_C08


title: Plate13_D23


title: Plate13_B10


title: Plate13_B11


title: Plate12_M06


title: Plate13_B07


title: Plate13_B08


title: Plate12_M07


title: Plate13_B13


title: Plate13_B09


title: Plate13_B15


title: Plate13_B12


title: Plate13_B14


title: Plate12_M10

value: Perfluorodecanoic acid_1_uM


title: Plate12_M13


title: Plate12_M09


title: Plate12_M11


title: Plate12_M12


title: Plate12_M15


title: Plate12_M16


title: Plate12_M05


title: Plate12_M04


title: Plate12_M18


title: Plate12_M23


title: Plate12_M19


title: Plate12_N03


title: Plate12_M20


title: Plate12_M08


title: Plate12_M21


title: Plate12_M22


title: Plate12_N07


title: Plate12_N02


title: Plate12_N06


title: Plate12_N08

value: Perfluorodecanoic acid_0.2_uM


title: Plate12_N05


title: Plate12_N10


title: Plate12_N13


title: Plate12_N11


title: Plate12_N12


title: Plate12_N09


title: Plate12_N14


title: Plate12_M14


title: Plate12_N15


title: Plate12_M17


title: Plate12_N17


title: Plate12_N16


title: Plate12_N18


title: Plate12_N21


title: Plate12_N04


title: Plate12_F11


title: Plate12_N23


title: Plate12_N22


title: Plate12_F14


title: Plate12_F12


title: Plate12_F16


title: Plate12_F18


title: Plate12_F15


title: Plate12_F19


title: Plate12_F17

value: Perfluorooctanesulfonic acid_10_uM


title: Plate12_F22

value: Folic acid_10_uM


title: Plate12_F21


title: Plate12_G02


title: Plate12_F20


title: Plate12_G04


title: Plate12_F23

value: Propamocarb hydrochloride_10_uM


title: Plate12_G05


title: Plate12_G03


title: Plate12_G06

value: Difenzoquat metilsulfate_1_uM


title: Plate12_G07


title: Plate12_N20


title: Plate12_G09


title: Plate12_G08


title: Plate12_G11


title: Plate12_G10


title: Plate12_N19


title: Plate12_F13

value: Diallyl phthalate_10_uM


title: Plate12_G14


title: Plate12_G12


title: Plate12_G13

value: Diallyl phthalate_1_uM


title: Plate12_G16


title: Plate12_G17

value: Perfluorooctanesulfonic acid_1_uM


title: Plate12_G18


title: Plate12_G23

value: Propamocarb hydrochloride_1_uM


title: Plate12_H02


title: Plate12_G15


title: Plate12_G21


title: Plate12_G19


title: Plate12_G20


title: Plate12_H04


title: Plate12_H10


title: Plate12_H11


title: Plate12_H07


title: Plate12_H05


title: Plate12_G22

value: Folic acid_1_uM


title: Plate12_H12


title: Plate12_H08


title: Plate12_H06

value: Difenzoquat metilsulfate_0.2_uM


title: Plate12_H18


title: Plate12_H13

value: Diallyl phthalate_0.2_uM


title: Plate12_H15


title: Plate12_H17

value: Perfluorooctanesulfonic acid_0.2_uM


title: Plate12_H19


title: Plate12_H16


title: Plate12_H14


title: Plate12_I02


title: Plate12_H20


title: Plate12_H22

value: Folic acid_0.2_uM


title: Plate12_H03

value: iPSC-Cardiomyocytes


title: Plate12_I03


title: Plate12_H21


title: Plate12_I07


title: Plate12_H09


title: Plate12_I05


title: Plate12_I06

value: Difenzoquat metilsulfate_10_uM


title: Plate12_I08


title: Plate12_I09


title: Plate12_I12


title: Plate12_I10


title: Plate12_I11


title: Plate12_I13


title: Plate12_I16


title: Plate12_I14


title: Plate12_I15


title: Plate12_I17


title: Plate12_I18


title: Plate12_I19


title: Plate12_H23

value: Propamocarb hydrochloride_0.2_uM


title: Plate12_I22


title: Plate12_I23


title: Plate12_I20


title: Plate12_J02


title: Plate12_J03


title: Plate12_J12


title: Plate12_J15


title: Plate12_J13


title: Plate12_J14


title: Plate12_J08


title: Plate12_J04


title: Plate12_J07


title: Plate12_I04


title: Plate12_J11


title: Plate12_J06


title: Plate12_J05


title: Plate12_J10


title: Plate12_J16


title: Plate12_J18


title: Plate12_J19


title: Plate12_J20


title: Plate12_J22


title: Plate12_J23


title: Plate12_J21


title: Plate12_J17


title: Plate12_K05


title: Plate12_I21


title: Plate12_K09


title: Plate12_K03


title: Plate12_K06


title: Plate12_K04


title: Plate12_K08


title: Plate12_K07


title: Plate12_J09


title: Plate12_K10


title: Plate12_K11


title: Plate12_K02


title: Plate12_K14


title: Plate12_K18


title: Plate12_K12


title: Plate12_L02


title: Plate12_K15


title: Plate12_L07


title: Plate12_K16


title: Plate12_L05


title: Plate12_K17


title: Plate12_K20


title: Plate12_L06


title: Plate12_K22


title: Plate12_K19


title: Plate12_L03


title: Plate12_K13


title: Plate12_L04


title: Plate12_L09

value: Perfluorodecanoic acid_10_uM


title: Plate12_L10


title: Plate12_K23


title: Plate12_L15


title: Plate12_L12


title: Plate12_L11


title: Plate12_L19


title: Plate12_L14


title: Plate12_L13


title: Plate12_K21


title: Plate12_L23


title: Plate12_L16


title: Plate12_M03


title: Plate12_L22


title: Plate12_L18


title: Plate12_L21


title: Plate12_B02


title: Plate12_L20


title: Plate12_B05


title: Plate12_L08


title: Plate12_M02


title: Plate12_B06


title: Plate12_B03


title: Plate12_B08


title: Plate12_B09


title: Plate12_B07


title: Plate12_L17


title: Plate12_B12


title: Plate12_B10


title: Plate12_B13


title: Plate12_B04


title: Plate12_B18


title: Plate12_B15


title: Plate12_B11


title: Plate12_B17


title: Plate12_B16


title: Plate12_B20


title: Plate12_B19


title: Plate12_B14


title: Plate12_B23


title: Plate12_B21


title: Plate12_C02


title: Plate12_C07


title: Plate12_C09


title: Plate12_C06


title: Plate12_C05


title: Plate12_C08


title: Plate12_C13


title: Plate12_C10


title: Plate12_C14


title: Plate12_C16


title: Plate12_C17

value: Perfluorooctanesulfonic acid_10_uM


title: Plate12_C18


title: Plate12_C19


title: Plate12_B22


title: Plate12_C22

value: Folic acid_10_uM


title: Plate12_C20


title: Plate12_C23

value: Propamocarb hydrochloride_10_uM


title: Plate12_C21


title: Plate12_D02


title: Plate12_C04


title: Plate12_D03


title: Plate12_D08


title: Plate12_D05


title: Plate12_C03


title: Plate12_D04


title: Plate12_D06

value: Difenzoquat metilsulfate_1_uM


title: Plate12_C11


title: Plate12_D07


title: Plate12_C12


title: Plate12_D12


title: Plate12_D14


title: Plate12_D13


title: Plate12_D11


title: Plate12_D16


title: Plate12_C15


title: Plate12_D15


title: Plate12_D17


title: Plate12_D18


title: Plate12_D19


title: Plate12_D21


title: Plate12_D20


title: Plate12_D22


title: Plate12_D23


title: Plate12_D09


title: Plate12_E05


title: Plate12_E02


title: Plate12_E03


title: Plate12_E07


title: Plate12_E08


title: Plate12_D10


title: Plate12_E06


title: Plate12_E14


title: Plate12_E11


title: Plate12_E12


title: Plate12_E10


title: Plate12_E19


title: Plate12_E15


title: Plate12_E20


title: Plate12_E17


title: Plate12_E16


title: Plate12_E13


title: Plate12_E21


title: Plate12_E18


title: Plate12_E22


title: Plate12_E23


title: Plate12_F02


title: Plate12_F03


title: Plate12_F05


title: Plate12_F04


title: Plate12_F07


title: Plate12_E09


title: Plate12_F06


title: Plate12_F08


title: Plate12_F10


title: Plate11_N18


title: Plate11_N19


title: Plate12_F09


title: Plate11_N22

value: Tris(2-chloroethyl) phosphate_0.2_uM


title: Plate11_N20


title: Plate11_N21


title: Plate12_E04


title: Plate11_N23


title: Plate11_M15


title: Plate11_M16


title: Plate11_M17


title: Plate11_M20

value: Tris(2-chloroethyl) phosphate_1_uM


title: Plate11_M23


title: Plate11_M21


title: Plate11_M19


title: Plate11_M18


title: Plate11_M22


title: Plate11_N03


title: Plate11_N02


title: Plate11_N04


title: Plate11_N05


title: Plate11_N10

value: Propyl gallate_0.2_uM


title: Plate11_N08


title: Plate11_N09


title: Plate11_N06


title: Plate11_N12


title: Plate11_N07


title: Plate11_N11


title: Plate11_N13


title: Plate11_N14


title: Plate11_N15


title: Plate11_N16


title: Plate11_N17


title: Plate11_L22

value: Tris(2-chloroethyl) phosphate_10_uM


title: Plate11_L21


title: Plate11_L17


title: Plate11_L16


title: Plate11_M03


title: Plate11_L18


title: Plate11_M05


title: Plate11_M06


title: Plate11_L20


title: Plate11_L19


title: Plate11_M07


title: Plate11_M08

value: Propyl gallate_1_uM


title: Plate11_L23


title: Plate11_M04


title: Plate11_K21


title: Plate11_K23


title: Plate11_M09


title: Plate11_M12


title: Plate11_M13


title: Plate11_K22


title: Plate11_K20


title: Plate11_L06


title: Plate11_M02


title: Plate11_L02


title: Plate11_L07


title: Plate11_L09

value: Propyl gallate_10_uM


title: Plate11_L03


title: Plate11_L05


title: Plate11_L10


title: Plate11_L11


title: Plate11_M10


title: Plate11_M11


title: Plate11_L12


title: Plate11_K02


title: Plate11_L08


title: Plate11_L13


title: Plate11_K04


title: Plate11_L15


title: Plate11_M14


title: Plate11_L14


title: Plate11_J23


title: Plate11_K03


title: Plate11_K06


title: Plate11_K07


title: Plate11_K08


title: Plate11_L04


title: Plate11_K10


title: Plate11_K17


title: Plate11_K11


title: Plate11_K14


title: Plate11_K16


title: Plate11_K15


title: Plate11_K12


title: Plate11_K18


title: Plate11_J07


title: Plate11_J09

value: Rhodamine 6G_1_uM


title: Plate11_J10


title: Plate11_J08


title: Plate11_J14


title: Plate11_J11


title: Plate11_J13

value: Diphenhydramine hydrochloride_1_uM


title: Plate11_J16


title: Plate11_J12


title: Plate11_K05


title: Plate11_J17


title: Plate11_J15


title: Plate11_J18


title: Plate11_J20


title: Plate11_K09

value: Rhodamine 6G_0.2_uM


title: Plate11_J19

value: iPSC-Cardiomyocytes


title: Plate11_I06


title: Plate11_J21


title: Plate11_K13

value: Diphenhydramine hydrochloride_0.2_uM


title: Plate11_I05


title: Plate11_K19


title: Plate11_J22


title: Plate11_I08


title: Plate11_I07


title: Plate11_I09

value: Rhodamine 6G_10_uM


title: Plate11_I10


title: Plate11_I12


title: Plate11_I11


title: Plate11_I14


title: Plate11_I13

value: Diphenhydramine hydrochloride_10_uM


title: Plate11_I16


title: Plate11_I17


title: Plate11_I15


title: Plate11_I18


title: Plate11_I20


title: Plate11_I21


title: Plate11_I19


title: Plate11_J03


title: Plate11_J05


title: Plate11_J04


title: Plate11_J02


title: Plate11_G16


title: Plate11_G14


title: Plate11_I22


title: Plate11_G15


title: Plate11_G18


title: Plate11_G17


title: Plate11_G21


title: Plate11_G20


title: Plate11_G19


title: Plate11_G22


title: Plate11_G23


title: Plate11_H02


title: Plate11_H05


title: Plate11_H03


title: Plate11_H04


title: Plate11_H07


title: Plate11_H08


title: Plate11_H09


title: Plate11_H06


title: Plate11_I23


title: Plate11_J06


title: Plate11_H11


title: Plate11_H10


title: Plate11_H12


title: Plate11_H14


title: Plate11_H17


title: Plate11_H15


title: Plate11_H16


title: Plate11_H20


title: Plate11_H18


title: Plate11_H21


title: Plate11_H23


title: Plate11_H22


title: Plate11_F09


title: Plate11_I03


title: Plate11_I04


title: Plate11_H19


title: Plate11_F10


title: Plate11_I02


title: Plate11_F11


title: Plate11_F14


title: Plate11_F15


title: Plate11_F13


title: Plate11_F16


title: Plate11_H13


title: Plate11_F17


title: Plate11_F18


title: Plate11_F20


title: Plate11_F21


title: Plate11_G05


title: Plate11_F23


title: Plate11_G03


title: Plate11_F22


title: Plate11_G04


title: Plate11_G02


title: Plate11_G07


title: Plate11_G08


title: Plate11_G06


title: Plate11_G10


title: Plate11_E16


title: Plate11_G11


title: Plate11_E13


title: Plate11_F12


title: Plate11_G12


title: Plate11_G13


title: Plate11_E18


title: Plate11_E14


title: Plate11_F19


title: Plate11_E19


title: Plate11_E22


title: Plate11_E21


title: Plate11_F05


title: Plate11_G09


title: Plate11_F07


title: Plate11_F04


title: Plate11_E23


title: Plate11_F03


title: Plate11_F06


title: Plate11_F02


title: Plate11_D17


title: Plate11_F08


title: Plate11_E17


title: Plate11_D19


title: Plate11_D18


title: Plate11_E15


title: Plate11_D23


title: Plate11_E20


title: Plate11_E07


title: Plate11_E04


title: Plate11_E03


title: Plate11_D22


title: Plate11_E06


title: Plate11_E05


title: Plate11_E10


title: Plate11_E09


title: Plate11_D20


title: Plate11_E08


title: Plate11_D03


title: Plate11_C22


title: Plate11_D04


title: Plate11_D07


title: Plate11_D06


title: Plate11_D05


title: Plate11_C23


title: Plate11_D21


title: Plate11_D08


title: Plate11_D02


title: Plate11_D11


title: Plate11_D10


title: Plate11_D12


title: Plate11_E11


title: Plate11_E02


title: Plate11_D13


title: Plate11_D09


title: Plate11_E12


title: Plate11_D16


title: Plate11_C08


title: Plate11_D15


title: Plate11_C10


title: Plate11_C12


title: Plate11_C11


title: Plate11_C07


title: Plate11_C09


title: Plate11_C13


title: Plate11_C14


title: Plate11_C19


title: Plate11_C17


title: Plate11_C16


title: Plate11_B15


title: Plate11_C21


title: Plate11_C15


title: Plate11_B14


title: Plate11_B23


title: Plate11_C18


title: Plate11_D14


title: Plate11_B21


title: Plate11_B18


title: Plate11_B20


title: Plate11_B22


title: Plate11_C03


title: Plate11_C06


title: Plate11_B19


title: Plate11_B02


title: Plate11_C04


title: Plate11_C05


title: Plate11_B06


title: Plate11_B05


title: Plate11_B07


title: Plate11_B16


title: Plate11_B10


title: Plate11_B11


title: Plate11_B12


title: Plate11_C20


title: Plate11_B08


title: Plate10_N22


title: Plate11_B17


title: Plate11_B03


title: Plate11_B09


title: Plate10_N23


title: Plate10_I15

value: Geranyl acetate_10_uM


title: Plate11_B04


title: Plate11_C02


title: Plate10_I16


title: Plate10_I17


title: Plate10_I18

value: Chlorhexidine diacetate_10_uM


title: Plate10_I21


title: Plate10_I22

value: Nicotinic acid_10_uM


title: Plate11_B13


title: Plate10_I23


title: Plate10_I14


title: Plate10_J04

value: Bisphenol B_1_uM


title: Plate10_J03


title: Plate10_J06


title: Plate10_J05


title: Plate10_J07


title: Plate10_J10


title: Plate10_J09

value: 4,4'-Diaminobiphenyl methane_1_uM


title: Plate10_J08


title: Plate10_J12


title: Plate10_J13


title: Plate10_J11


title: Plate10_J14


title: Plate10_J17


title: Plate10_J15

value: Geranyl acetate_1_uM


title: Plate10_J18

value: Chlorhexidine diacetate_1_uM


title: Plate10_J02


title: Plate10_I19


title: Plate10_J16


title: Plate10_J22

value: Nicotinic acid_1_uM


title: Plate10_J21


title: Plate10_J20

value: Methyl salicylate_1_uM


title: Plate10_J19


title: Plate10_J23


title: Plate10_K05


title: Plate10_K06


title: Plate10_K02


title: Plate10_K07


title: Plate10_K04

value: Bisphenol B_0.2_uM


title: Plate10_K03


title: Plate10_K10


title: Plate10_K08


title: Plate10_K09

value: 4,4'-Diaminobiphenyl methane_0.2_uM


title: Plate10_K11


title: Plate10_K12


title: Plate10_K13


title: Plate10_K14


title: Plate10_K19


title: Plate10_K15

value: Geranyl acetate_0.2_uM


title: Plate10_K17


title: Plate10_K16


title: Plate10_K21


title: Plate10_K22

value: Nicotinic acid_0.2_uM


title: Plate10_K20

value: iPSC-Cardiomyocytes

value: Methyl salicylate_0.2_uM


title: Plate10_L02


title: Plate10_K23


title: Plate10_L07


title: Plate10_K18

value: Chlorhexidine diacetate_0.2_uM


title: Plate10_L10


title: Plate10_L06


title: Plate10_L12


title: Plate10_L15


title: Plate10_L04


title: Plate10_L08


title: Plate10_L03


title: Plate10_L05


title: Plate10_L09


title: Plate10_L19


title: Plate10_L14


title: Plate10_L13


title: Plate10_L23


title: Plate10_L18


title: Plate10_L11


title: Plate10_L16


title: Plate10_M04


title: Plate10_L20


title: Plate10_L22


title: Plate10_M02


title: Plate10_M05


title: Plate10_M03


title: Plate10_M07


title: Plate10_L17


title: Plate10_M08


title: Plate10_M10


title: Plate10_M12


title: Plate10_L21


title: Plate10_M14


title: Plate10_M15


title: Plate10_M11


title: Plate10_M13


title: Plate10_M16


title: Plate10_M18


title: Plate10_M17


title: Plate10_M19


title: Plate10_M21


title: Plate10_M20


title: Plate10_M22


title: Plate10_M09


title: Plate10_M23


title: Plate10_N03


title: Plate10_N02


title: Plate10_M06


title: Plate10_N04


title: Plate10_N05


title: Plate10_N06


title: Plate10_N10


title: Plate10_N09


title: Plate10_N08


title: Plate10_N11


title: Plate10_N13


title: Plate10_N14


title: Plate10_N15


title: Plate10_N12


title: Plate10_N17


title: Plate10_N16


title: Plate10_N18


title: Plate10_N20


title: Plate10_D10


title: Plate10_N19


title: Plate10_D13


title: Plate10_N21


title: Plate10_D11


title: Plate10_D12


title: Plate10_N07


title: Plate10_D14


title: Plate10_D16


title: Plate10_D15


title: Plate10_D17


title: Plate10_D22


title: Plate10_E02


title: Plate10_E03


title: Plate10_D20


title: Plate10_D23


title: Plate10_E05


title: Plate10_E07


title: Plate10_E06


title: Plate10_E08


title: Plate10_E09


title: Plate10_E11


title: Plate10_E12


title: Plate10_E10


title: Plate10_E14


title: Plate10_E15


title: Plate10_E13


title: Plate10_D19


title: Plate10_E17


title: Plate10_D18


title: Plate10_E16


title: Plate10_E20


title: Plate10_E19


title: Plate10_E18


title: Plate10_I20

value: Methyl salicylate_10_uM


title: Plate10_D21


title: Plate10_E23


title: Plate10_E21


title: Plate10_E22


title: Plate10_F03


title: Plate10_F04

value: Bisphenol B_10_uM


title: Plate10_E04


title: Plate10_F05


title: Plate10_F06


title: Plate10_F12


title: Plate10_F11


title: Plate10_F13


title: Plate10_F09

value: 4,4'-Diaminobiphenyl methane_10_uM


title: Plate10_F16


title: Plate10_F17


title: Plate10_F10


title: Plate10_F02


title: Plate10_F14


title: Plate10_F15

value: Geranyl acetate_10_uM


title: Plate10_F18

value: Chlorhexidine diacetate_10_uM


title: Plate10_F08


title: Plate10_G04

value: Bisphenol B_1_uM


title: Plate10_F21


title: Plate10_G05


title: Plate10_F07


title: Plate10_G03


title: Plate10_F19


title: Plate10_F23


title: Plate10_F20


title: Plate10_G07


title: Plate10_G09

value: 4,4'-Diaminobiphenyl methane_1_uM


title: Plate10_F22

value: Nicotinic acid_10_uM


title: Plate10_G06


title: Plate10_G11


title: Plate10_G08


title: Plate10_G12


title: Plate10_G10


title: Plate10_G13


title: Plate10_G17


title: Plate10_G02


title: Plate10_G14


title: Plate10_G16


title: Plate10_G15

value: Geranyl acetate_1_uM


title: Plate10_G18

value: Chlorhexidine diacetate_1_uM


title: Plate10_G19


title: Plate10_G20

value: Methyl salicylate_1_uM


title: Plate10_G21


title: Plate10_G22

value: Nicotinic acid_1_uM


title: Plate10_H05


title: Plate10_H02


title: Plate10_H06


title: Plate10_H08


title: Plate10_H03


title: Plate10_H13


title: Plate10_H04

value: Bisphenol B_0.2_uM


title: Plate10_H07


title: Plate10_H10


title: Plate10_H14


title: Plate10_H09

value: 4,4'-Diaminobiphenyl methane_0.2_uM


title: Plate10_H17

value: iPSC-Cardiomyocytes


title: Plate10_H16


title: Plate10_H15

value: Geranyl acetate_0.2_uM


title: Plate10_H19


title: Plate10_G23


title: Plate10_H20

value: Methyl salicylate_0.2_uM


title: Plate10_H21


title: Plate10_H22

value: Nicotinic acid_0.2_uM


title: Plate10_I04

value: Bisphenol B_10_uM


title: Plate10_I02


title: Plate10_H23


title: Plate10_I03


title: Plate10_I06


title: Plate10_I08


title: Plate10_I07


title: Plate10_H12


title: Plate10_H11


title: Plate10_I12


title: Plate10_H18

value: Chlorhexidine diacetate_0.2_uM


title: Plate10_B02


title: Plate10_I11


title: Plate10_I09

value: 4,4'-Diaminobiphenyl methane_10_uM


title: Plate10_B03


title: Plate10_B06


title: Plate10_B04


title: Plate10_I13


title: Plate10_B05


title: Plate10_B11


title: Plate10_B07


title: Plate10_B08


title: Plate10_I05


title: Plate10_B09


title: Plate10_B16


title: Plate10_B12


title: Plate10_B17


title: Plate10_B13


title: Plate10_B15


title: Plate10_B18


title: Plate10_I10


title: Plate10_B22


title: Plate10_B20


title: Plate10_B19


title: Plate10_C03


title: Plate10_B10


title: Plate10_C07


title: Plate10_B23


title: Plate10_C08


title: Plate10_B21


title: Plate10_C06


title: Plate10_C05


title: Plate10_C10


title: Plate10_C02


title: Plate10_C11


title: Plate10_C04


title: Plate10_C13


title: Plate10_C15

value: Geranyl acetate_10_uM


title: Plate10_C14


title: Plate10_C17


title: Plate10_C12


title: Plate10_C18

value: Chlorhexidine diacetate_10_uM


title: Plate10_C20

value: Methyl salicylate_10_uM


title: Plate10_B14


title: Plate10_C16


title: Plate10_D09

value: 4,4'-Diaminobiphenyl methane_1_uM


title: Plate10_D08


title: Plate10_C21


title: Plate10_C19


title: Plate9_I15


title: Plate9_I16

value: Tributyl phosphate_10_uM


title: Plate10_C22

value: Nicotinic acid_10_uM


title: Plate10_C23


title: Plate10_D02


title: Plate10_C09


title: Plate9_I19

value: Bisphenol A_10_uM


title: Plate9_I20


title: Plate10_D03


title: Plate9_I22


title: Plate10_D04

value: Bisphenol B_1_uM


title: Plate10_D06


title: Plate9_I21

value: Diisobutyl phthalate_10_uM


title: Plate10_D07


title: Plate9_N20


title: Plate10_D05


title: Plate9_I14

value: Dibutyl adipate_10_uM


title: Plate9_N22

value: iPSC-Cardiomyocytes


title: Plate9_N21


title: Plate9_N23


title: Plate9_J02


title: Plate9_I17


title: Plate9_J04

value: Nalidixic acid_1_uM


title: Plate9_J08


title: Plate9_J07


title: Plate9_J06

value: Perfluorooctanoic acid_1_uM


title: Plate9_I18


title: Plate9_J03


title: Plate9_J14

value: Dibutyl adipate_1_uM


title: Plate9_J05


title: Plate9_J18


title: Plate9_J16

value: Tributyl phosphate_1_uM


title: Plate9_J09


title: Plate9_J10


title: Plate9_J12


title: Plate9_J15


title: Plate9_K03


title: Plate9_J11


title: Plate9_I23


title: Plate9_K04

value: Nalidixic acid_0.2_uM


title: Plate9_K07


title: Plate9_K09


title: Plate9_K08


title: Plate9_K12


title: Plate9_K10


title: Plate9_J19

value: Bisphenol A_1_uM


title: Plate9_K13


title: Plate9_J21

value: Diisobutyl phthalate_1_uM


title: Plate9_J22


title: Plate9_J20


title: Plate9_J23


title: Plate9_K06

value: Perfluorooctanoic acid_0.2_uM


title: Plate9_K05


title: Plate9_K19

value: Bisphenol A_0.2_uM


title: Plate9_K18


title: Plate9_K22


title: Plate9_L02

value: Dimethyl succinate_10_uM


title: Plate9_K11


title: Plate9_L03


title: Plate9_L07


title: Plate9_J17


title: Plate9_J13


title: Plate9_K14

value: Dibutyl adipate_0.2_uM


title: Plate9_L11


title: Plate9_L12


title: Plate9_K15


title: Plate9_K02


title: Plate9_K17


title: Plate9_K16

value: Tributyl phosphate_0.2_uM


title: Plate9_K20


title: Plate9_K21

value: Diisobutyl phthalate_0.2_uM


title: Plate9_L19


title: Plate9_K23


title: Plate9_L21


title: Plate9_L04


title: Plate9_L06


title: Plate9_L05


title: Plate9_L08


title: Plate9_L13


title: Plate9_M04

value: Dimethyl succinate_1_uM


title: Plate9_M03


title: Plate9_L09


title: Plate9_M07


title: Plate9_L14


title: Plate9_L15


title: Plate9_L16


title: Plate9_M12


title: Plate9_L18


title: Plate9_M11


title: Plate9_L23


title: Plate9_L20


title: Plate9_L22


title: Plate9_L17


title: Plate9_L10


title: Plate9_M02


title: Plate9_M16


title: Plate9_M05


title: Plate9_M20


title: Plate9_M08


title: Plate9_M09


title: Plate9_M06


title: Plate9_M22


title: Plate9_M10


title: Plate9_M13


title: Plate9_N06


title: Plate9_N08


title: Plate9_M19


title: Plate9_M14


title: Plate9_N14


title: Plate9_N15


title: Plate9_M15


title: Plate9_M18


title: Plate9_M17


title: Plate9_M21


title: Plate9_N02

value: Dimethyl succinate_0.2_uM


title: Plate9_N17


title: Plate9_N03


title: Plate9_M23


title: Plate9_N19


title: Plate9_D11


title: Plate9_N04


title: Plate9_N05


title: Plate9_N07


title: Plate9_N09


title: Plate9_N10


title: Plate9_D15


title: Plate9_N13


title: Plate9_D18


title: Plate9_D17


title: Plate9_N12


title: Plate9_D19


title: Plate9_N16


title: Plate9_D20


title: Plate9_N18


title: Plate9_D23


title: Plate9_E03


title: Plate9_D09


title: Plate9_D10


title: Plate9_D12


title: Plate9_E07


title: Plate9_N11


title: Plate9_E08


title: Plate9_E11


title: Plate9_D14


title: Plate9_E12


title: Plate9_D16


title: Plate9_E13


title: Plate9_D21


title: Plate9_E15


title: Plate9_D22


title: Plate9_E05


title: Plate9_E04


title: Plate9_E02


title: Plate9_E18


title: Plate9_E06


title: Plate9_E09


title: Plate9_E23


title: Plate9_D13


title: Plate9_F06

value: Perfluorooctanoic acid_10_uM


title: Plate9_F05

value: iPSC-Cardiomyocytes


title: Plate9_E10


title: Plate9_E14


title: Plate9_E17


title: Plate9_F10


title: Plate9_E20


title: Plate9_E19


title: Plate9_F09


title: Plate9_F02


title: Plate9_F12


title: Plate9_E22


title: Plate9_F03


title: Plate9_F18


title: Plate9_F19

value: Bisphenol A_10_uM


title: Plate9_F13


title: Plate9_F21

value: Diisobutyl phthalate_10_uM


title: Plate9_F22


title: Plate9_F07


title: Plate9_G03


title: Plate9_E16


title: Plate9_F11


title: Plate9_F08


title: Plate9_G07


title: Plate9_G08


title: Plate9_G10


title: Plate9_F14

value: Dibutyl adipate_10_uM


title: Plate9_F15


title: Plate9_G11


title: Plate9_G12


title: Plate9_E21


title: Plate9_F16

value: Tributyl phosphate_10_uM


title: Plate9_G16

value: Tributyl phosphate_1_uM


title: Plate9_F20


title: Plate9_F04

value: Nalidixic acid_10_uM


title: Plate9_G17


title: Plate9_G14

value: Dibutyl adipate_1_uM


title: Plate9_F23


title: Plate9_G21

value: Diisobutyl phthalate_1_uM


title: Plate9_G04

value: Nalidixic acid_1_uM


title: Plate9_G22


title: Plate9_G02


title: Plate9_H02


title: Plate9_G23


title: Plate9_G05


title: Plate9_G06

value: Perfluorooctanoic acid_1_uM


title: Plate9_F17


title: Plate9_H03


title: Plate9_G09


title: Plate9_G13


title: Plate9_G15


title: Plate9_G18


title: Plate9_G20


title: Plate9_G19

value: Bisphenol A_1_uM


title: Plate9_H15


title: Plate9_H16

value: Tributyl phosphate_0.2_uM


title: Plate9_H05


title: Plate9_H18


title: Plate9_H17


title: Plate9_H08


title: Plate9_H06

value: Perfluorooctanoic acid_0.2_uM


title: Plate9_H10


title: Plate9_H21

value: Diisobutyl phthalate_0.2_uM


title: Plate9_H11


title: Plate9_H23


title: Plate9_I02


title: Plate9_H14

value: Dibutyl adipate_0.2_uM


title: Plate9_H13


title: Plate9_H19

value: Bisphenol A_0.2_uM


title: Plate9_H04

value: iPSC-Cardiomyocytes

value: Nalidixic acid_0.2_uM


title: Plate9_H20


title: Plate9_H09


title: Plate9_H22


title: Plate9_I04


title: Plate9_H07


title: Plate9_H12


title: Plate9_I03


title: Plate9_I05


title: Plate9_I07


title: Plate9_I08


title: Plate9_I10


title: Plate9_I11


title: Plate9_I09


title: Plate9_I13


title: Plate9_B04


title: Plate9_I12


title: Plate9_B02


title: Plate9_B09


title: Plate9_B06


title: Plate9_B07


title: Plate9_B05


title: Plate9_B10


title: Plate9_B08


title: Plate9_I06

value: Perfluorooctanoic acid_10_uM


title: Plate9_B11


title: Plate9_B12


title: Plate9_B13


title: Plate9_B14


title: Plate9_B15


title: Plate9_B16


title: Plate9_B17


title: Plate9_B19


title: Plate9_B20


title: Plate9_B03


title: Plate9_B22


title: Plate9_C06


title: Plate9_B21


title: Plate9_B23


title: Plate9_C02


title: Plate9_C05


title: Plate9_C04


title: Plate9_B18


title: Plate9_C08


title: Plate9_C17


title: Plate9_C07


title: Plate9_C10


title: Plate9_C19

value: Bisphenol A_10_uM


title: Plate9_C18


title: Plate9_C21

value: Diisobutyl phthalate_10_uM


title: Plate9_C11


title: Plate9_C12


title: Plate9_D03


title: Plate9_C13


title: Plate9_C14

value: Dibutyl adipate_10_uM


title: Plate9_D05


title: Plate9_C15


title: Plate9_C16

value: Tributyl phosphate_10_uM


title: Plate9_D07


title: Plate9_C20


title: Plate8_J15


title: Plate9_C03


title: Plate8_J16


title: Plate9_C23


title: Plate9_C22


title: Plate9_C09


title: Plate8_J20


title: Plate9_D04


title: Plate8_J23


title: Plate9_D02


title: Plate8_K02


title: Plate8_K05

value: Denatonium saccharide_0.2_uM


title: Plate9_D06


title: Plate8_K06


title: Plate8_K04


title: Plate8_J18

value: Potassium perfluorooctanesulfonate_1_uM


title: Plate9_D08


title: Plate8_J19


title: Plate8_K07

value: Perfluorononanoic acid_0.2_uM


title: Plate8_J22


title: Plate8_J21


title: Plate8_K12


title: Plate8_K03


title: Plate8_K08

value: Tamoxifen citrate_0.2_uM


title: Plate8_K21


title: Plate8_K11


title: Plate8_K09


title: Plate8_K13


title: Plate8_L03


title: Plate8_K22


title: Plate8_J17


title: Plate8_L04


title: Plate8_L08


title: Plate8_L09


title: Plate8_L06


title: Plate8_K10

value: p-Xylenol blue_0.2_uM


title: Plate8_K16


title: Plate8_L14


title: Plate8_K15


title: Plate8_K17


title: Plate8_K14


title: Plate8_K18


title: Plate8_K19


title: Plate8_K20


title: Plate8_L02


title: Plate8_L15


title: Plate8_L05


title: Plate8_L10


title: Plate8_K23


title: Plate8_L07


title: Plate8_L11


title: Plate8_L13


title: Plate8_L20


title: Plate8_L22


title: Plate8_L19


title: Plate8_L23


title: Plate8_M04


title: Plate8_L17


title: Plate8_M07


title: Plate8_L18


title: Plate8_L21


title: Plate8_L16


title: Plate8_M02


title: Plate8_L12


title: Plate8_M06


title: Plate8_M08


title: Plate8_M05


title: Plate8_M12


title: Plate8_M13


title: Plate8_M03


title: Plate8_M10


title: Plate8_M09


title: Plate8_M17


title: Plate8_M20


title: Plate8_M22


title: Plate8_M23


title: Plate8_M14


title: Plate8_M15


title: Plate8_N04


title: Plate8_M11


title: Plate8_M18


title: Plate8_M19


title: Plate8_N03


title: Plate8_M21


title: Plate8_N13


title: Plate8_N02


title: Plate8_N08


title: Plate8_N06


title: Plate8_N17


title: Plate8_N19


title: Plate8_M16


title: Plate8_N12


title: Plate8_N16


title: Plate8_N09


title: Plate8_N07


title: Plate8_N21


title: Plate8_N10


title: Plate8_N05


title: Plate8_N20


title: Plate8_N23


title: Plate8_E08


title: Plate8_E06


title: Plate8_N11


title: Plate8_E09


title: Plate8_E07


title: Plate8_N18


title: Plate8_E10


title: Plate8_N14


title: Plate8_E12


title: Plate8_N22


title: Plate8_E16


title: Plate8_E15


title: Plate8_E17


title: Plate8_N15


title: Plate8_E11


title: Plate8_E14


title: Plate8_E20


title: Plate8_E22


title: Plate8_E23


title: Plate8_E18


title: Plate8_F02


title: Plate8_E21


title: Plate8_F07

value: Perfluorononanoic acid_10_uM


title: Plate8_E19


title: Plate8_F03


title: Plate8_F08

value: Tamoxifen citrate_10_uM


title: Plate8_F04


title: Plate8_F10

value: p-Xylenol blue_10_uM


title: Plate8_F05

value: Denatonium saccharide_10_uM


title: Plate8_F06


title: Plate8_F13


title: Plate8_F12


title: Plate8_E13


title: Plate8_F11


title: Plate8_F09


title: Plate8_F14


title: Plate8_F18


title: Plate8_F15


title: Plate8_F16


title: Plate8_F17


title: Plate8_G04


title: Plate8_F20


title: Plate8_F19


title: Plate8_F22


title: Plate8_G11


title: Plate8_F23


title: Plate8_G02


title: Plate8_G12


title: Plate8_G05

value: Denatonium saccharide_1_uM


title: Plate8_G06

value: iPSC-Cardiomyocytes


title: Plate8_G03


title: Plate8_G07

value: Perfluorononanoic acid_1_uM


title: Plate8_G15


title: Plate8_G13


title: Plate8_G09


title: Plate8_G19


title: Plate8_G20


title: Plate8_G08

value: Tamoxifen citrate_1_uM


title: Plate8_G10

value: p-Xylenol blue_1_uM


title: Plate8_G16


title: Plate8_G23


title: Plate8_H02


title: Plate8_G21


title: Plate8_F21


title: Plate8_G18


title: Plate8_G22


title: Plate8_G17


title: Plate8_G14


title: Plate8_H05


title: Plate8_H04


title: Plate8_H07


title: Plate8_H06


title: Plate8_H08


title: Plate8_H10


title: Plate8_H09


title: Plate8_H13


title: Plate8_H12


title: Plate8_H11


title: Plate8_H03


title: Plate8_H15


title: Plate8_H16


title: Plate8_H19


title: Plate8_H23


title: Plate8_H17


title: Plate8_H20


title: Plate8_I04


title: Plate8_H21


title: Plate8_I05


title: Plate8_I02


title: Plate8_I03


title: Plate8_H18


title: Plate8_I06


title: Plate8_I07


title: Plate8_I09


title: Plate8_I08


title: Plate8_H22


title: Plate8_I14


title: Plate8_I13


title: Plate8_I11


title: Plate8_I12


title: Plate8_H14


title: Plate8_I15


title: Plate8_I16


title: Plate8_I10


title: Plate8_I17


title: Plate8_I20


title: Plate8_I19


title: Plate8_I21


title: Plate8_I22


title: Plate8_J02


title: Plate8_J03


title: Plate8_J08


title: Plate8_J06


title: Plate8_J07


title: Plate8_J04


title: Plate8_J05


title: Plate8_J12


title: Plate8_B05


title: Plate8_J13


title: Plate8_B03


title: Plate8_B06


title: Plate8_J10


title: Plate8_J14


title: Plate8_B07


title: Plate8_B02


title: Plate8_B08


title: Plate8_J09


title: Plate8_B12


title: Plate8_B09


title: Plate8_I23


title: Plate8_B14


title: Plate8_I18


title: Plate8_B10


title: Plate8_B11


title: Plate8_B04


title: Plate8_B17


title: Plate8_B21


title: Plate8_B23


title: Plate8_C04


title: Plate8_C02


title: Plate8_B16


title: Plate8_B15


title: Plate8_J11


title: Plate8_C09


title: Plate8_B18


title: Plate8_B13


title: Plate8_B19


title: Plate8_B20


title: Plate8_C11


title: Plate8_C12


title: Plate8_C05


title: Plate8_C13


title: Plate8_C06


title: Plate8_C18


title: Plate8_C17


title: Plate8_C20


title: Plate8_C19


title: Plate8_B22


title: Plate8_C10


title: Plate8_C08


title: Plate8_C07


title: Plate8_C14


title: Plate8_C22


title: Plate8_C23


title: Plate8_C15


title: Plate8_D07


title: Plate8_C16


title: Plate8_D09


title: Plate8_C03


title: Plate8_C21


title: Plate8_D03


title: Plate8_D02


title: Plate8_D05


title: Plate8_D11


title: Plate8_D06


title: Plate8_D04


title: Plate8_D12


title: Plate8_D15


title: Plate8_D23


title: Plate8_D14


title: Plate8_E02


title: Plate8_D16


title: Plate8_D08


title: Plate7_N09


title: Plate8_D17


title: Plate8_D18


title: Plate8_D21


title: Plate8_D20


title: Plate8_D22


title: Plate8_E04


title: Plate8_E03


title: Plate8_E05


title: Plate7_N08


title: Plate7_N15


title: Plate7_N16


title: Plate8_D13


title: Plate8_D10


title: Plate7_N20


title: Plate7_N19


title: Plate7_N10


title: Plate7_N11

value: FD&C Green No. 3_0.2_uM


title: Plate7_M13

value: FD&C Green No. 3_1_uM


title: Plate8_D19


title: Plate7_N12


title: Plate7_N14


title: Plate7_M14


title: Plate7_N18


title: Plate7_M15


title: Plate7_N13


title: Plate7_N17


title: Plate7_N22


title: Plate7_M17


title: Plate7_M19


title: Plate7_M18


title: Plate7_N23


title: Plate7_M21


title: Plate7_N21


title: Plate7_M12


title: Plate7_M20


title: Plate7_M22


title: Plate7_N04


title: Plate7_N05


title: Plate7_M23


title: Plate7_N02


title: Plate7_N06


title: Plate7_N07


title: Plate7_L17


title: Plate7_L16


title: Plate7_M16


title: Plate7_L18


title: Plate7_L19


title: Plate7_M02


title: Plate7_M04


title: Plate7_L22


title: Plate7_L20


title: Plate7_L23


title: Plate7_L21


title: Plate7_M05

value: Potassium perfluorohexanesulfonate_1_uM


title: Plate7_N03


title: Plate7_M07


title: Plate7_M09


title: Plate7_M08


title: Plate7_M10


title: Plate7_K20


title: Plate7_M11


title: Plate7_K23


title: Plate7_K22


title: Plate7_K19


title: Plate7_L03


title: Plate7_L04


title: Plate7_K21


title: Plate7_L07


title: Plate7_L06


title: Plate7_L02


title: Plate7_L09


title: Plate7_M03


title: Plate7_L05


title: Plate7_L10


title: Plate7_M06


title: Plate7_L12

value: FD&C Green No. 3_10_uM


title: Plate7_L14


title: Plate7_L15


title: Plate7_J20


title: Plate7_J22


title: Plate7_J21


title: Plate7_J23


title: Plate7_K02


title: Plate7_K03


title: Plate7_K05

value: Adipic acid_0.2_uM


title: Plate7_K07


title: Plate7_K06


title: Plate7_K10


title: Plate7_K08


title: Plate7_K04


title: Plate7_K11


title: Plate7_K13

value: Triphenyl phosphate_0.2_uM


title: Plate7_L11


title: Plate7_L08


title: Plate7_K09


title: Plate7_K17


title: Plate7_K14


title: Plate7_K16


title: Plate7_L13


title: Plate7_K12


title: Plate7_K15


title: Plate5_C08


title: Plate5_C11


title: Plate5_C07


title: Plate5_C10


title: Plate5_C05


title: Plate7_K18

value: Chlorpromazine hydrochloride_0.2_uM


title: Plate5_C06


title: Plate5_C09


title: Plate5_C14


title: Plate5_C15


title: Plate5_C13


title: Plate5_C17


title: Plate5_C16


title: Plate5_C19


title: Plate5_C20


title: Plate5_C21


title: Plate5_C23


title: Plate5_D03

value: Ammonium perfluorooctanoate_1_uM


title: Plate5_D04


title: Plate5_D06


title: Plate5_C22


title: Plate5_D02


title: Plate5_D05


title: Plate5_D08


title: Plate5_D09


title: Plate5_D07


title: Plate5_D11


title: Plate5_C12

value: L-Tartaric acid_10_uM


title: Plate5_D10


title: Plate5_D12

value: L-Tartaric acid_1_uM


title: Plate5_D15


title: Plate5_D14


title: Plate5_D13


title: Plate5_D16


title: Plate5_D17


title: Plate5_D18


title: Plate5_D19

value: Dipropyl 2,5-pyridinedicarboxylate_1_uM


title: Plate5_D21


title: Plate5_D20


title: Plate5_C18


title: Plate5_D22


title: Plate5_E07


title: Plate5_E02


title: Plate5_E08


title: Plate5_E09


title: Plate5_E10


title: Plate5_E05


title: Plate5_E04


title: Plate5_E13


title: Plate5_E15


title: Plate5_E14


title: Plate5_E17


title: Plate5_E16


title: Plate5_E18


title: Plate5_E19


title: Plate5_B02


title: Plate5_B08


title: Plate5_B06


title: Plate5_B05


title: Plate5_D23


title: Plate5_B04


title: Plate5_B03


title: Plate5_E03

value: Ammonium perfluorooctanoate_0.2_uM


title: Plate5_B07


title: Plate5_B09


title: Plate5_B10


title: Plate5_E06


title: Plate5_B12


title: Plate5_E11


title: Plate5_B11


title: Plate5_E12

value: L-Tartaric acid_0.2_uM


title: Plate5_B13

value: iPSC-Cardiomyocytes


title: Plate5_B16


title: Plate5_B14


title: Plate5_B18


title: Plate5_B19


title: Plate5_B15


title: Plate5_B17


title: Plate5_B20


title: Plate5_B21


title: Plate5_B23


title: Plate5_C04


title: Plate5_C03

value: Ammonium perfluorooctanoate_10_uM


title: Plate5_C02


title: Plate5_B22


title: Plate4_L09


title: Plate4_L10


title: Plate4_L11


title: Plate4_L07


title: Plate4_L13


title: Plate4_L12


title: Plate4_L15


title: Plate4_L17

value: 6:2 Fluorotelomer methacrylate_10_uM


title: Plate4_L19


title: Plate4_L16


title: Plate4_L18


title: Plate4_L14


title: Plate4_L20


title: Plate4_M03


title: Plate4_M06


title: Plate4_L21


title: Plate4_L23


title: Plate4_M02


title: Plate4_L22


title: Plate4_M08


title: Plate4_M05


title: Plate4_L08


title: Plate4_M09


title: Plate4_M10


title: Plate4_M11


title: Plate4_M12


title: Plate4_M15


title: Plate4_M14


title: Plate4_M16


title: Plate4_M17

value: 6:2 Fluorotelomer methacrylate_1_uM


title: Plate4_M19


title: Plate4_M20


title: Plate4_M18


title: Plate4_M23


title: Plate4_M04


title: Plate4_M07


title: Plate4_N02


title: Plate4_M22


title: Plate4_N04


title: Plate4_N05


title: Plate4_M21


title: Plate4_N11


title: Plate4_N07


title: Plate4_N08


title: Plate4_N09


title: Plate4_N03


title: Plate4_N06


title: Plate4_M13


title: Plate4_N13


title: Plate4_N10


title: Plate4_N12


title: Plate4_N15


title: Plate4_N16


title: Plate4_N17

value: 6:2 Fluorotelomer methacrylate_0.2_uM


title: Plate4_N18


title: Plate4_N14


title: Plate4_N19


title: Plate4_N20


title: Plate4_N21


title: Plate4_H21


title: Plate4_N22


title: Plate4_I02


title: Plate4_I05


title: Plate4_H22


title: Plate4_I03


title: Plate4_N23


title: Plate4_I06

value: 2,4-Dichlorophenoxyacetic acid_10_uM


title: Plate4_H23


title: Plate4_I13


title: Plate4_I07

value: Piperonyl butoxide_10_uM


title: Plate4_I09


title: Plate4_I11


title: Plate4_I14


title: Plate4_I16


title: Plate4_I12


title: Plate4_I18


title: Plate4_I10


title: Plate4_I19


title: Plate4_I15

value: Perfluorohexanoic acid_10_uM


title: Plate4_I20


title: Plate4_J02


title: Plate4_I17


title: Plate4_I21


title: Plate4_I04


title: Plate4_J04


title: Plate4_J05


title: Plate4_J07

value: Piperonyl butoxide_1_uM


title: Plate4_J09


title: Plate4_J11


title: Plate4_J03


title: Plate4_J10


title: Plate4_J06

value: 2,4-Dichlorophenoxyacetic acid_1_uM


title: Plate4_J12


title: Plate4_J14


title: Plate4_J15

value: Perfluorohexanoic acid_1_uM


title: Plate4_J13


title: Plate4_J16


title: Plate4_J21


title: Plate4_I23


title: Plate4_I22


title: Plate4_J17


title: Plate4_K02


title: Plate4_K06

value: 2,4-Dichlorophenoxyacetic acid_0.2_uM


title: Plate4_K07

value: Piperonyl butoxide_0.2_uM


title: Plate4_K10


title: Plate4_J20


title: Plate4_J19


title: Plate4_J22


title: Plate4_K12


title: Plate4_K14


title: Plate4_K03


title: Plate4_J23


title: Plate4_K15

value: Perfluorohexanoic acid_0.2_uM


title: Plate4_J18


title: Plate4_K16


title: Plate4_K09


title: Plate4_K19


title: Plate4_K13


title: Plate4_K11


title: Plate4_K22


title: Plate4_K04


title: Plate4_K05


title: Plate4_L02


title: Plate4_L06


title: Plate4_K17


title: Plate4_K20


title: Plate4_K23


title: Plate4_F03


title: Plate4_L03


title: Plate4_K18


title: Plate4_L04


title: Plate4_K21


title: Plate4_L05


title: Plate4_F11


title: Plate4_F13


title: Plate4_E21


title: Plate4_E23


title: Plate4_E22


title: Plate4_F02


title: Plate4_F18


title: Plate4_F17


title: Plate4_F15


title: Plate4_F04


title: Plate4_F20


title: Plate4_F09


title: Plate4_F22


title: Plate4_F21


title: Plate4_F06


title: Plate4_F23


title: Plate4_F12


title: Plate4_F05


title: Plate4_F19


title: Plate4_F14


title: Plate4_F16


title: Plate4_G02


title: Plate4_F07


title: Plate4_G05


title: Plate4_G03


title: Plate4_G04


title: Plate4_F10


title: Plate4_G06


title: Plate4_G12


title: Plate4_G13


title: Plate4_G11


title: Plate4_G07


title: Plate4_G09


title: Plate4_G10


title: Plate4_G15


title: Plate4_G17


title: Plate4_G19


title: Plate4_G20


title: Plate4_G23


title: Plate4_G18


title: Plate4_G16


title: Plate4_H05


title: Plate4_H03


title: Plate4_H02


title: Plate4_H09


title: Plate4_H10


title: Plate4_H04


title: Plate4_H15


title: Plate4_H06


title: Plate4_H16


title: Plate4_H12


title: Plate4_H07


title: Plate4_H18


title: Plate4_H19


title: Plate4_H14


title: Plate4_C04


title: Plate4_H11


title: Plate4_H13


title: Plate4_G14


title: Plate4_G22


title: Plate4_H20


title: Plate4_C06


title: Plate4_H17


title: Plate4_G21


title: Plate4_C05


title: Plate4_C09


title: Plate4_C13


title: Plate4_C11


title: Plate4_C10


title: Plate4_C12


title: Plate4_C16


title: Plate4_C14


title: Plate4_C15


title: Plate4_C17


title: Plate4_C20


title: Plate4_C21


title: Plate4_C22


title: Plate4_D02


title: Plate4_C23


title: Plate4_D04


title: Plate4_D03


title: Plate4_D07


title: Plate4_D05


title: Plate4_C07


title: Plate4_D10


title: Plate4_D06


title: Plate4_D12


title: Plate4_D13


title: Plate4_D15


title: Plate4_D11


title: Plate4_D17


title: Plate4_D18


title: Plate4_D14


title: Plate4_D20


title: Plate4_D16


title: Plate4_C19


title: Plate4_C18


title: Plate4_D19


title: Plate4_E04


title: Plate4_D23


title: Plate4_E02


title: Plate4_D21


title: Plate4_D22


title: Plate4_E05


title: Plate4_D09


title: Plate4_E06


title: Plate4_E11


title: Plate4_E15


title: Plate4_E07


title: Plate4_E16


title: Plate4_E09


title: Plate4_E12


title: Plate4_E14


title: Plate4_E13


title: Plate4_E17


title: Plate4_B02


title: Plate4_E20


title: Plate4_E03


title: Plate4_B06


title: Plate4_B03


title: Plate4_E18


title: Plate4_E19


title: Plate4_B08


title: Plate4_B11


title: Plate4_B05


title: Plate4_B09


title: Plate4_B04


title: Plate4_B07


title: Plate4_B12


title: Plate4_B10


title: Plate4_B14


title: Plate4_B13


title: Plate4_B17


title: Plate4_B19


title: Plate4_B16


title: Plate4_B18


title: Plate4_E10


title: Plate4_C02


title: Plate4_B23


title: Plate4_B22


title: Plate4_B15


title: Plate4_B21


title: Plate3_K05


title: Plate4_C03


title: Plate4_B20


title: Plate3_J22


title: Plate3_K08


title: Plate3_K15


title: Plate3_K02


title: Plate3_K03


title: Plate3_K18

value: Dibutyl phthalate_0.2_uM


title: Plate3_K16


title: Plate3_K19


title: Plate3_K04


title: Plate3_K07


title: Plate3_K06


title: Plate3_K23

value: Testosterone propionate_0.2_uM


title: Plate3_K09


title: Plate3_K10


title: Plate3_K12


title: Plate3_L03


title: Plate3_L04


title: Plate3_K13

value: Dibutyl decanedioate_0.2_uM


title: Plate3_L06

value: iPSC-Cardiomyocytes

value: Methyl 2-aminobenzoate_10_uM


title: Plate3_K14


title: Plate3_L09

value: Triglycidyl isocyanurate_10_uM


title: Plate3_L11


title: Plate3_L05


title: Plate3_L16


title: Plate3_L12


title: Plate3_L18

value: Di(2-ethylhexyl) phthalate_10_uM


title: Plate3_J23

value: Testosterone propionate_1_uM


title: Plate3_K20


title: Plate3_K21


title: Plate3_K22


title: Plate3_L02


title: Plate3_L23


title: Plate3_L22


title: Plate3_K11


title: Plate3_L07


title: Plate3_M05

value: Methyl 2-aminobenzoate_1_uM


title: Plate3_L08


title: Plate3_L10


title: Plate3_M07


title: Plate3_K17


title: Plate3_L13


title: Plate3_L14


title: Plate3_M09

value: Triglycidyl isocyanurate_1_uM


title: Plate3_M11


title: Plate3_L15


title: Plate3_M13


title: Plate3_L17


title: Plate3_M12


title: Plate3_M16


title: Plate3_M23


title: Plate3_L20


title: Plate3_L21


title: Plate3_M03


title: Plate3_M02


title: Plate3_N05

value: Methyl 2-aminobenzoate_0.2_uM


title: Plate3_N10

value: Triglycidyl isocyanurate_0.2_uM


title: Plate3_N11


title: Plate3_M04


title: Plate3_N12


title: Plate3_M18

value: Di(2-ethylhexyl) phthalate_1_uM


title: Plate3_L19


title: Plate3_M06


title: Plate3_M08


title: Plate3_M10


title: Plate3_N16


title: Plate3_N15


title: Plate3_M14


title: Plate3_N19

value: Di(2-ethylhexyl) phthalate_0.2_uM


title: Plate3_M17


title: Plate3_M15


title: Plate3_M21


title: Plate3_N17


title: Plate3_M20


title: Plate3_M22


title: Plate3_F11


title: Plate3_N03


title: Plate3_N04


title: Plate3_N06


title: Plate3_F14


title: Plate3_N07


title: Plate3_N02


title: Plate3_N08


title: Plate3_M19


title: Plate3_N13


title: Plate3_F21


title: Plate3_F19


title: Plate3_N09


title: Plate3_N18


title: Plate3_N20


title: Plate3_N23


title: Plate3_G03


title: Plate3_N21


title: Plate3_N22


title: Plate3_F10


title: Plate3_G05


title: Plate3_G04


title: Plate3_G06


title: Plate3_F13

value: Dibutyl decanedioate_10_uM


title: Plate3_G08


title: Plate3_F15


title: Plate3_F16


title: Plate3_G09


title: Plate3_N14


title: Plate3_F12

value: Dodecyltrimethylammonium chloride_10_uM


title: Plate3_F17


title: Plate3_G12

value: Dodecyltrimethylammonium chloride_1_uM


title: Plate3_G10


title: Plate3_G11


title: Plate3_F18

value: Dibutyl phthalate_10_uM


title: Plate3_G14


title: Plate3_F22


title: Plate3_F23

value: Testosterone propionate_10_uM


title: Plate3_G16


title: Plate3_F20


title: Plate3_G02


title: Plate3_G07


title: Plate3_G19


title: Plate3_G22


title: Plate3_G20


title: Plate3_G15


title: Plate3_G18

value: Dibutyl phthalate_1_uM


title: Plate3_G13

value: iPSC-Cardiomyocytes

value: Dibutyl decanedioate_1_uM


title: Plate3_G23


title: Plate3_H02


title: Plate3_G21


title: Plate3_H04


title: Plate3_H05


title: Plate3_H06


title: Plate3_H09


title: Plate3_H11


title: Plate3_H08


title: Plate3_H10


title: Plate3_H12


title: Plate3_H13

value: Dibutyl decanedioate_0.2_uM


title: Plate3_H07


title: Plate3_H14


title: Plate3_H15


title: Plate3_H16


title: Plate3_H17


title: Plate3_H18

value: Dibutyl phthalate_0.2_uM


title: Plate3_H19


title: Plate3_H03


title: Plate3_H22


title: Plate3_H23

value: Testosterone propionate_0.2_uM


title: Plate3_I02


title: Plate3_I04


title: BAL_Rep_2

city: Covington

first: Alexandra

last: Melton

address: 18703 Three Rivers Road

institute: Tulane National Primate Research Center

description: Parse Single-Cell Whole Transcriptome Kit

library_source: transcriptomic single cell

data_processing:
Initial sequencing data in BCL format were generated using the NextSeq 2000 platform. These files were subsequently converted to FASTQ format via Illumina BaseSpace and transferred to a dedicated server for further processing. A combined indexed reference genome was constructed, incorporating sequences from Rhesus macaque, SIV, and SARS-CoV-2, using the Split-Pipe pipeline (version 0.9.6 (Rep 1) or 1.0.3 (Rep 2)). This pipeline was then employed to process each sublibrary independently, performing the following steps: barcode identification and error correction, sequence read alignment, transcript counts assigned to genes, generation of cell-by-gene count matrices, and production of summary statistics and visualizations. Following individual sublibrary processing, the respective Split-Pipe pipeline was utilized to combine the outputs of each sublibrary into a single dataset. This integration process involved merging data and statistical files, resulting in the generation of a new summary report. The combined results were stored in a newly generated output directory.
scRNAseq data analysis was performed using the Seurat package in R [33]. The cell-gene matrix (DGE.mtx), cell metadata (cell_metadata.csv), and all genes files (all_genes.csv) generated from both experimental runs using the Parse Biosciences pipeline were imported into R using the readMM and read.delim functions.
Assembly: Macaca mulatta (GCA_003339765.3), SARS-CoV-2 genome (GCA_009858895.3), and SIV genome (GenBank Accession # M33262.1)
Supplementary files format and content: Comma-separated values files and matrix files

organism: Macaca nemestrina

source_name: Bronchoalveolar lavage (BAL)

extract_protocol:
For single-cell sequencing of bronchoalveolar lavage (BAL) cells, we collected samples before SARS-CoV-2 inoculation and on days 2, 7, 21, and 28 post-challenge. BAL samples were centrifuged at room temperature for 5 min at 1800 rpm, and the resulting cell pellets were resuspended in DMEM supplemented with 10% FBS and 1% Anti-Anti. We used the Parse Biosciences cell fixation kit, following the manufacturer’s instructions for PBMCs, to fix the cells (Parse Biosciences, Seattle, WA, USA). Specifically, we fixed 1 million cells per animal/timepoint in a 15 mL falcon tube. The fixed cells were stored at −20 °C until all samples were collected.
To enable multiplexing of samples, the Parse Single-Cell whole transcriptome kit, which utilizes a combinatorial barcoding approach (Evercode WT, Parse Biosciences, USA), was employed following the ParseBiosciences Evercode WT v2 User Manual v2.3 . This allowed us to barcode and multiplex 10 samples collected from the coinfected animals across five timepoints.

tag: tissue

value: Bronchoalveolar lavage (BAL)

value: Immune cells


title: CD8_LSDI1_rep3

city: milano

first: Saverio

last: Minucci

address: via Adamello 16

country: Italy

institute: European Institute of Oncology

data_processing:
Raw reads were mapped to the mouse reference genome mm9 using STAR
Reads quantification was calculated using the featureCount
Batch correction of the two runs of the NT-LSDi samples was assessed with the R package sva
edgeR was used to assess differential expression. Differentially expressed genes (DEGs) were defined as those showing FDR ≤0.05 and linear fold-change ≥1.2
Assembly: mm9
Supplementary files format and content: tab-delimited file text file includes raw counts for each sample
Supplementary files format and content: tab-delimited file text file includes bacth-corrected counts for each sample

organism: Mus musculus

source_name: CD8+ cells

growth_protocol: T cells were resuspended at 1x106cells/mL in complete RPMI media (10% FBS, 1% NaPy, 1% Glutamine, 1% Pen/Strep, 0,1% β-mercaptoethanol) supplemented with 100U/mL IL2.

extract_protocol:
RNA was isolated from T cells using RNeasy Mini Kits (Qiagen). 1ug of total RNA was used for the construction of the libraries.
RNAseq libraries were prepared using SMARTER mRNA-Seq Library Preparation Kit

treatment_protocol: CD8+ cells were activated for 72h in presence or absence of LSDi

value: CD8+ cells

value: LSD1 inhibitor


title: Plate3_I07


title: CD8_LSDI1_rep2


title: CD8_LSDI1_rep1


title: BAL_Rep_1


title: Plate3_H20


title: Plate3_H21


title: CD8_NT_rep3

value: not treated


title: Plate3_I11


title: Plate3_I09


title: Plate3_I12


title: CD8_NT_rep4


title: Plate3_I08


title: Plate3_I10


title: CD8_LSDI1_rep4


title: CD8_NT_rep5


title: Plate3_G17


title: Plate3_I13


title: Plate3_I15


title: Plate3_I03


title: Plate3_I05


title: Plate3_I06


title: Plate3_I14


title: Plate3_I16


title: Plate3_I17


title: E15.5 Six2-Cre; Irx3/5(loxP/loxP) 4

city: Toronto

first: Norman

middle: D

last: Rosenblum

address: 686 Bay St

country: Canada

institute: The Hospital for Sick Children

data_processing:
Data quality was assessed using FastQC v.0.11.5.
Adaptors and sequencing reads were trimmed using Trim Galore v.0.5.0. Trimmed reads were re-assessed using FastQC.
The raw trimmed reads were aligned to the reference genome using STAR aligner v.2.6.0c.
The filtered STAR alignments were processed to extract raw read counts for genes using htseq-count v.0.6.1p2.
Differential gene analysis was performed with DESeq2 v.1.26.0s.
Assembly: mm39 (GRCm39)
Supplementary files format and content: tab-delimited text file containing the following: GeneID, GeneName, baseMean, log2Foldchange, lfcSE, Wald statistic, Wald p-value, B-H adjusted p-value, FPKM values for each sample

source_name: E15.5 whole embryonic kidney

extract_protocol:
RNA was extracted from whole kidneys using the Qiagen RNEasy Mini Kit
RNA libraries for RNA-seq were prepared using the NEB Ultra II Directional RNA Library Prep Kit following manufacturer's protocols.

value: E15.5 whole embryonic kidney

tag: genotype

value: Mut


title: E15.5 Six2-Cre; Irx3/5(loxP/loxP) 2


title: E15.5 Six2-Cre; Irx3/5(loxP/loxP) 3


title: Plate3_I18


title: CD8_NT_rep2


title: CD8_NT_rep1


title: E15.5 Irx3/5(loxP/+) 2

value: WT


title: Plate3_I20


title: Plate3_I21


title: Plate3_I22


title: MS, NT MAG Exomyo, 3 injections, biol rep 1

city: Milano

first: Yvan

last: Torrente

address: Via Festa del Perdono, 7

institute: Università degli Studi di Milano

data_processing:
To ensure the quality of the experiment, the FASTQ were checked through FastQC (v0.12.0)
Trimmed sequences were quasi-mapped to GrCM38/mm10 using salmon version 1.10.1
Genes were considered expressed if at least 20 raw counts in 2 out of three replicates were present.
TMM normalization was performed using edgeR(version 3.42.0).
Genes were considered as differentially expressed if their logFC was > (<) log2(1.5) and FDR < 0.05 using edgeR likelihood ratio test.
Assembly: GRCm38
Supplementary files format and content: tab-delmited text file includes raw counts for each sample

source_name: Skeletal muscle

extract_protocol:
RNeasy Micro kit (QIAGEN).
RNAs were qualified and quantified with AGILENT Tapestation 2200 on High Sensitivity RNA chip. To prepare the library and sequence the DNA, 150 to 300 ng of total RNA from samples was determined by InvitrogenTM QubitTM; high-sensitivity spectrofluorometric measurement was poly-A selected and reverse transcribed using Illumina’s TruSeq stranded mRNA library preparation kit. Each sample was fitted with one of 96 adapters containing a different 8 base molecular barcode for high level multiplexing and libraries were sequenced on an Illumina NovaSeqTM 6000.

value: Skeletal muscle

value: MS

value: NT MAG Exomyo


title: E15.5 Irx3/5(loxP/+) 1


title: Plate3_I23

value: Testosterone propionate_10_uM


title: Plate3_J03

value: iPSC-Cardiomyocytes


title: E15.5 Irx3/5(loxP/+) 3


title: Plate3_J05


title: MS, NT Exomyo, 3injection, biol rep 3

value: NT Exomyo


title: Plate3_J04


title: MS, NT MAG Exomyo, 3 injections, biol rep 2


title: MS, NT Exomyo, 3injection, biol rep 1


title: Plate3_J06


title: MAC cells, NT MAG Exomyo, 3 injections, biol rep 3

value: MAC


title: Plate3_J07


title: MS, NT MAG Exomyo, 3 injections, biol rep 3


title: Plate3_I19


title: MAC cells, NT MAG Exomyo, 3 injections, biol rep 2


title: Plate3_J08


title: E15.5 Six2-Cre; Irx3/5(loxP/loxP) 1


title: MAC cells, NT Exomyo, 3injection, biol rep 3


title: Plate3_J10


title: Plate3_J11


title: Plate3_J02


title: MAC cells, NT Exomyo, 3injection, biol rep 1


title: MAC cells, NT Exomyo, 3injection,biol rep 2


title: Plate3_J12

value: Dodecyltrimethylammonium chloride_1_uM


title: Plate3_J14


title: Plate3_J15


title: Plate3_J16


title: MS, NT MAG Exomyo, 2 injections, biol rep 1


title: Plate3_J18

value: Dibutyl phthalate_1_uM


title: MS, NT MAG Exomyo, 2 injections, biol rep 2


title: MS, NT MAG Exomyo, 2 injections, biol rep 3


title: Plate3_J21


title: MAC cells, NT MAG Exomyo, 3 injections, biol rep 1


title: MS, NT MAG Exomyo, 1injection, biol rep 2


title: Plate3_J20


title: Plate3_J19


title: Plate3_J09


title: MS, Veh, biol rep 3

value: Vehicle


title: Plate3_J17


title: Plate3_B02


title: MS, Veh, biol rep 2


title: MS, NT MAG Exomyo, 1injection, biol rep 3


title: MS, NT Exomyo, 3injection, biol rep 2


title: Plate3_J13

value: Dibutyl decanedioate_1_uM


title: MS, NT MAG Exomyo, 1injection, biol rep 1


title: Plate3_B03


title: MS, Veh, biol rep 1


title: Plate3_B05


title: Plate3_B04


title: MAC cells, NT MAG Exomyo, 2 injections, biol rep 3


title: Plate3_B07


title: Plate3_B06


title: Plate3_B11


title: Plate3_B10


title: Plate3_B09


title: MAC cells, NT MAG Exomyo, 2 injections, biol rep 1


title: Plate3_B12


title: MAC cells, NT MAG Exomyo, 2 injections, biol rep 2


title: MAC cells, NT MAG Exomyo, 1injection, biol rep 2


title: MAC cells, NT MAG Exomyo, 1injection, biol rep 1


title: Plate3_B13


title: MAC cells, NT MAG Exomyo, 1injection, biol rep 3


title: Plate3_B16


title: Plate3_B14


title: MAC cells, Veh, biol rep 3


title: Plate3_B15


title: 24 week MASH

city: New York

first: Chittampalli

middle: Narayanan

last: Yashaswini

state: NY

address: 1425 Madison Avenue, 11-76

department: Liver

institute: Icahn School of Medicine at Mount Sinai

description: 10x genomics

data_processing:
Gene Expression and Hashtag Libraries have been processed withCell Ranger v5.0.1andaligned torefdata-gex-GRCh38-2020-A
Assembly: mm10-2020-A
Supplementary files format and content: Tab-separated values files and matrix files

source_name: Liver

extract_protocol:
One gram of fresh liver from MASH mice and age-matched healthy mice were harvested and kept in RPMI + 10% fetal bovine serum (FBS) on ice overnight. The following morning, livers were injected with digestion buffer (RPMI + 10% FBS, 0.25mL Collagenase IV and 0.1mg/mL DNase I), minced and incubated at 37oC for 1 hour under slow agitation (80rpm). Samples were mechanically digested with 16G needles, filtered through 100uM and 70uM strainers, pelleted, resuspended in 25% Percoll, and layered atop 70% Percoll. Samples were centrifuged and non-parenchymal cells were collected from the meeting point of the Percoll layers, rinsed with 1x HBSS (no ions) and RBC lysis was performed.
Library was performed according to manufacturer's instructions (single cell, 10x 5' Chromium - GEX)

treatment_protocol: Eight-week-old male C57BL/6J mice from Jackson Laboratory were maintained on regular chow diet, or put on the Fibrosis and Tumor (FAT) MASH model, which involves administration of a Western diet of 21.1% fat, 41% sucrose, and 1.25% cholesterol (Teklad diets, 120528) with a sugar water solution consisting of 23.1g d-fructose/L and 18.9g d-glucose/L, combined with weekly intraperitoneal injections of CCl4 at the low dose of 0.2 ml (0.32 mg)/g of body weight.

value: Liver

value: Non-parenchymal cells

value: 24 weeks FAT MASH model


title: Plate3_B17


title: MAC cells, Veh, biol rep 1


title: Plate3_B18


title: MAC cells, Veh, biol rep 2


title: 12 week MASH

description: 10x genomics

value: 12 weeks FAT MASH model


title: Plate3_B20


title: Plate3_B19


title: 12 week chow

description: 10x genomics

value: 12 weeks of Chow diet


title: Tet_TKO_NSC_RNAseq_rep2

first: Meelad

middle: M

last: Dawlaty

state: New York

address: 1301 Morris Park Ave, Price 419, Bronx

department: Genetics

institute: Albert Einstein College of Medicine

description:
RNAseq_TKOvsWT_NSC_DESeq2_normalizedCounts.csv
M10_Ntkh7

data_processing:
For RNA-seq, libraries were sequenced using the Illumina Novoseq 6000 platform. Adaptor and low-quality trimming were performed with trim galore (v0.6.5, https://www.bioinformatics.babraham.ac.uk/projects/trim_galore/). Clean reads were mapped to Mus musculus reference genome mm10 using STAR (v2.7.3a) with default parameters. Counts were extracted from mapped reads with featureCounts using the –largestOverlap parameter. DESeq2 (v1.40.2) was used to identify differentially expressed genes (FDR < 0.05, fold change > 2) from raw counts using the standard package documentation
For WGBS, paired-end sequencing on a DNBseq platform was performed at BGI Genomics. Raw read filtering was performed by BGI Genomics using SOAPnuke with the parameters -n 0.001 -l 20 -q 0.4 –adaMR 0.25 –ada_trim –polyX 50 to remove adaptors and filter out low-quality reads. Clean reads were mapped to mm10 using Bismark (v0.22.3) with default parameters. Read deduplication was performed using deduplicate_bismark and cytosine methylation levels were extracted with bismark_methylation_extractor. BAM files were balanced to the sample with the lowest number of reads with SAMtools (v1.9)
For CUT&Tag, libraries were sequenced on an Illumina NextSeq 500 platform at the Einstein Epigenomics Shared Facility. Adaptor and low-quality trimming were performed with trim galore. Clean reads were mapped to mm10 using bowtie293 using options -local -sensitive -very-sensitive-local --no-unal -no-mixed -no-discordant -phred33 -I 10 -X 700. Duplicate reads were removed with Picard tools (v2.26.10, https://broadinstitute.github.io/picard/).
Samtools was used to convert SAM to BAM, sort and index corresponding BAM files. Bigwig files were generated using bamCoverage from deepTools, and bigwig files were analyzed using plotProfile and plotHeatmap. Peaks annotation were implemented by R package "ChIPseeker".
Assembly: mm10
Supplementary files format and content: bigwig
Supplementary files format and content: normalized gene counts
Supplementary files format and content: bedgraph

source_name: Neural stem cells

growth_protocol: ESCs preplated and cultured on gelatin in serum/LIF for 24 hours before harvest for high-throughput sequencing. NSCs were generated following the protocol of Okabe et al. (1996) as modified by Lee et al. (2000), passaged once, and then harvested for high-throughput sequencing.

extract_protocol:
For RNA-seq, RNA was extracted from ESCs and NSCs using Qiagen RNeasy Mini kit. For WGBS, DNA was extracted from ESCs and NSCs using Zymo Quick-DNA Miniprep Kit.
RNA-seq libraries were prepared by Novogene (https://en.novogene.com/). WGBS libraries were prepared by BGI Genomics (https://en.genomics.cn/). CUT&Tag libraries were prepared by NEBNext HiFi 2x PCR Master mix.

treatment_protocol: For CUT&Tag, ESC and NSCs were crosslinked, bound to Con-A beads, incubated with primary antibody and treated with pre-loaded pA-Tn5 adapter complex.

value: Neural stem cells

tag: Sex

value: Male

tag: strain

value: Tet triple knockout


title: 24 week chow

description: 10x genomics

value: 24 weeks of Chow diet


title: Plate3_B08


title: Tet_TKO_NSC_RNAseq_rep1

description:
RNAseq_TKOvsWT_NSC_DESeq2_normalizedCounts.csv
M9_Ntkc2


title: Plate3_B22


title: Plate3_B21


title: Plate3_B23


title: Plate3_C02


title: Tet_WT_NSC_RNAseq_rep2

description:
RNAseq_TKOvsWT_NSC_DESeq2_normalizedCounts.csv
M7_Nw2

value: Tet wildtype


title: Tet_TKO_ESC_RNAseq_rep2

description:
RNAseq_TKOvsWT_ESC_DESeq2_normalizedCounts.csv
M5_Etkh7

source_name: Embryonic stem cells

value: Embryonic stem cells


title: Tet_WT_ESC_RNAseq_rep3

description:
RNAseq_TKOvsWT_ESC_DESeq2_normalizedCounts.csv
M3_Ew3


title: Plate3_C04

value: iPSC-Cardiomyocytes


title: Plate3_C05


title: Plate3_C03


title: Plate3_C07


title: Tet_TKO_ESC_RNAseq_rep1

description:
RNAseq_TKOvsWT_ESC_DESeq2_normalizedCounts.csv
M4_Etkc2


title: Tet_WT_ESC_RNAseq_rep2

description:
RNAseq_TKOvsWT_ESC_DESeq2_normalizedCounts.csv
M2_Ew2


title: Plate3_C08


title: Plate3_C10


title: Plate3_C09


title: Plate3_C06


title: Tet_WT_ESC_RNAseq_rep1

description:
RNAseq_TKOvsWT_ESC_DESeq2_normalizedCounts.csv
M1_Ew1


title: D4_TKO_H3K27ac_HiChIP_rep1

city: Paris

first: Julien

last: Richard Albert

address: 15 Rue Hélène Brion

country: France

institute: Institut Jacques Monod, CNRS, Université de Paris

library_source: genomic

data_processing:
CUT&Run data: PCR duplicate removal using BBmap clumpify (Bushnell et al., 2014)(v38.18) and parameters: reorder dedupe=t k=19 passes=6 subs=(0.01 x readlength). Trimmomatic (Bolger et al., 2014) (v0.39) and parameters: CROP:36 ILLUMINACLIP:adapters.fa:2:30:10 SLIDINGWINDOW:4:20 MINLEN:24
Read pairs that survived trimming were aligned to the mouse reference genome (build mm10) using Bowtie2 (Langmead et al., 2019)(v2.4.5) for CUT&Run using parameters: --local –very-sensitive –no-mixed –dovetail –no-discordant –phred33 -I 10 -X 700
Processed bigwig files were generated using deeptools (Ramírez et al., 2016) bamCoverage (v3.5.1) and parameters: --binSize 1 –smoothLength 0 –minMappingQuality 10 –normalizeUsing CPM –outFileFormat bigwig –blackListFileName ENCFF547MET.bed –ignoreForNormalization chrX chrM chrY
HiChIP data: the HiC-Pro pipeline (v3.1.0) (Servant et al. 2015) was used to digest the mm10 genome (^GATC G^ANTC), align reads and generate contact map matrices. Single-end alignment bam files were used for peak calling using macs2 (v2.2.7.1) (Zhang et al. 2008) and generating bigwigs using bamCompare (as described above). Loops were called using hichipper (v0.7.7) (Lareau and Aryee 2018b) and parameters “-mu -pp 1000” and differential loops were calculated using diffloop (Lareau and Aryee 2018a) and quickAssoc normalization. bigInteract tracks of differential looping were colour coded based on statistical significance (FDR<0.05).
Assembly: mm10
Supplementary files format and content: Bigwig format. CUT&Run: CPM-normalized counts. HiChIP: CPM-normalized counts and bigBedInteract loop files (considering 1kb padding around achors)
Library strategy: HiChIP

molecule: genomic DNA

source_name: mouse embryonic stem cells

growth_protocol: Day 4 post-FGF2/Activin A treatment

extract_protocol:
Hi-ChIP experiments were performed using the Arima Hi-ChIP kit (Arima Genomics) according to the manufacturer’s instructions. 15g of chromatin were used per sample and experiments were performed in duplicates. Briefly, cells were cross-linked with 2% formaldehyde for 10 min at room temperature, lysed and chromatin was digested with two different restriction enzymes included in the kit. Overhangs were filled-in in the presence of biotinylated nucleotides, followed by ligation. Ligated DNA was sonicated using the Covaris M220 to an average fragment size of 500 bp with the following parameters (Peak incident power: 50; Duty factor: 10%; Cycles per burst: 200; Treatment time: 250s). DNA was then immunoprecipitated overnight using 2.5µg of H3K27Ac (Active Motif 91193) or CTCF antibody (Active Motif 91285). After a double-size selection to retain DNA fragments between 200 and 600 bp using Ampure XP beads (Beckman Coulter) the biotin-ligated DNA was precipitated with streptavidin-coupled magnetic beads (included in the kit). The Hi-ChIP libraries were prepared on beads using the Accel-NGS 2D library Kit (Swift bioscience) following instructions from the Arima Hi-ChIP kit. Final libraries were analyzed using 4200 TapeStation system (Agilent) and sequenced. The Hi-ChIP libraries were prepared on beads using the Accel-NGS 2D library Kit (Swift bioscience) following instructions from the Arima Hi-ChIP kit. Final libraries were analyzed using 4200 TapeStation system (Agilent) and sequenced.
The Hi-ChIP libraries were prepared on beads using the Accel-NGS 2D library Kit (Swift bioscience) following instructions from the Arima Hi-ChIP kit. Final libraries were analyzed using 4200 TapeStation system (Agilent) and sequenced. The Hi-ChIP libraries were prepared on beads using the Accel-NGS 2D library Kit (Swift bioscience) following instructions from the Arima Hi-ChIP kit. Final libraries were analyzed using 4200 TapeStation system (Agilent) and sequenced.

value: mouse embryonic stem cells

tag: growth media

value: N2B27 medium supplemented with 12 ng/mL FGF2 (R&D) and 20 ng/mL Activin A (R&D)

value: DnmtTKO


title: D4_TKO_H3K27ac_HiChIP_rep2


title: Plate3_C13

value: Dibutyl decanedioate_10_uM


title: Plate3_C11


title: D0_TKO_H3K27ac_HiChIP_rep1

growth_protocol: Day 0 post-FGF2/Activin A treatment


title: D4_WT_H3K27ac_HiChIP_rep1

value: DnmtWT


title: D4_WT_H3K27ac_HiChIP_rep2


title: Plate3_C12

value: Dodecyltrimethylammonium chloride_10_uM


title: D0_TKO_H3K27ac_HiChIP_rep2


title: Plate3_C15


title: Plate3_C14


title: Tet_WT_NSC_RNAseq_rep3

description:
RNAseq_TKOvsWT_NSC_DESeq2_normalizedCounts.csv
M8_Nw3


title: Tet_WT_NSC_RNAseq_rep1

description:
RNAseq_TKOvsWT_NSC_DESeq2_normalizedCounts.csv
M6_Nw1


title: D0_WT_H3K27ac_HiChIP_rep1


title: Plate3_C17


title: D4_TKO_CTCF_HiChIP_rep2


title: Plate3_C18

value: Dibutyl phthalate_10_uM


title: D4_TKO_CTCF_HiChIP_rep1


title: D4_WT_CTCF_HiChIP_rep2


title: Plate3_C19


title: D4_WT_CTCF_HiChIP_rep1


title: D0_TKO_CTCF_HiChIP_rep2


title: Plate3_C22


title: D0_TKO_CTCF_HiChIP_rep1


title: Plate3_C23

value: Testosterone propionate_10_uM


title: Plate3_C21


title: D0_WT_CTCF_HiChIP_rep2


title: Plate3_D02


title: Plate3_D03


title: Plate3_D04


title: D4_TKO_CTCF_CUTnRUN_rep2

data_processing:
CUT&Run data: PCR duplicate removal using BBmap clumpify (Bushnell et al., 2014)(v38.18) and parameters: reorder dedupe=t k=19 passes=6 subs=(0.01 x readlength). Trimmomatic (Bolger et al., 2014) (v0.39) and parameters: CROP:36 ILLUMINACLIP:adapters.fa:2:30:10 SLIDINGWINDOW:4:20 MINLEN:24
Read pairs that survived trimming were aligned to the mouse reference genome (build mm10) using Bowtie2 (Langmead et al., 2019)(v2.4.5) for CUT&Run using parameters: --local –very-sensitive –no-mixed –dovetail –no-discordant –phred33 -I 10 -X 700
Processed bigwig files were generated using deeptools (Ramírez et al., 2016) bamCoverage (v3.5.1) and parameters: --binSize 1 –smoothLength 0 –minMappingQuality 10 –normalizeUsing CPM –outFileFormat bigwig –blackListFileName ENCFF547MET.bed –ignoreForNormalization chrX chrM chrY
HiChIP data: the HiC-Pro pipeline (v3.1.0) (Servant et al. 2015) was used to digest the mm10 genome (^GATC G^ANTC), align reads and generate contact map matrices. Single-end alignment bam files were used for peak calling using macs2 (v2.2.7.1) (Zhang et al. 2008) and generating bigwigs using bamCompare (as described above). Loops were called using hichipper (v0.7.7) (Lareau and Aryee 2018b) and parameters “-mu -pp 1000” and differential loops were calculated using diffloop (Lareau and Aryee 2018a) and quickAssoc normalization. bigInteract tracks of differential looping were colour coded based on statistical significance (FDR<0.05).
Assembly: mm10
Supplementary files format and content: Bigwig format. CUT&Run: CPM-normalized counts. HiChIP: CPM-normalized counts and bigBedInteract loop files (considering 1kb padding around achors)
Library strategy: CUT&Run

extract_protocol:
We performed CUT&RUN according to the original protocol (Skene and Henikoff 2017) with the following modifications: 500,000 cells were used for each sample, and the primary antibody was incubated overnight at 4ºC on a rotator. After incubation with pAG-MNase and performing the MNAse reaction, the samples were placed on a magnetic rack and the supernatant containing the DNA samples was recovered. Following addition of 0.1% SDS and 0.17mg/ml Proteinase K, samples were incubated at 50°C for 1h. Purified DNA was obtained by phenol/chloroform extraction and precipitated with 100% Ethanol by centrifugation. The DNA pellet was washed in 80% ethanol, spun down and air-dried before being resuspended in 25µl of 1mM Tris-HCl pH8.0. We used a primary CTCF antibody (Cell D31H2) or IgG control (SigmaAldrich I5006) and no secondary antibody was used for these experiments. The pAG-MNase plasmid was obtained from Addgene (#123461), and the protein was purified by the Curiecoretech Recombinant Protein Platform.
Sequencing library preparation was made using the NEBNext® UltraTM II DNA Library Prep Kit for Illumina (NEB) following the procedure described in “Library Prep for CUT&RUN with NEBNext® UltraTM II DNA Library Prep Kit for Illumina® (E7645) V.1” available in protocols.io. Quality control for the finalized libraries was performed using a TapeStation 420 system (Agilent). Libraries were sequenced by Novogene Co on a NovaSeq using paired-end 150 base pair parameters, requesting 4 gigabytes of data per sample, or approximately 13 million reads.


title: D4_TKO_CTCF_CUTnRUN_rep1


title: D0_WT_H3K27ac_HiChIP_rep2


title: D4_IgG_CUTnRUN_rep1


title: Plate3_D06


title: D4_WT_CTCF_CUTnRUN_rep1


title: Plate3_D07


title: Plate3_D05


title: Plate3_C16


title: D4_WT_CTCF_CUTnRUN_rep2


title: D0_IgG_CUTnRUN_rep1


title: Plate3_D08


title: Plate3_D12

value: Dodecyltrimethylammonium chloride_1_uM


title: Plate3_C20


title: D0_TKO_CTCF_CUTnRUN_rep1


title: D0_WT_CTCF_CUTnRUN_rep2


title: Plate3_D11


title: D0_WT_CTCF_CUTnRUN_rep1


title: D0_WT_CTCF_HiChIP_rep1


title: D0_TKO_CTCF_CUTnRUN_rep2


title: Plate3_D14


title: Plate3_D15


title: Plate3_D09


title: Plate3_D13

value: Dibutyl decanedioate_1_uM


title: Plate3_D16


title: Cortex Desmin 12

city: Boston

first: Sarah

last: Pfau

state: MA

address: 220 Longwood Avenue

institute: Harvard Medical School

data_processing:
save-interim-files=false
quality-trim-score=20
2color-trimming=True
adapter1=AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
adapter2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT
adapter-trim-match-length=10
adapter-trim-max-mismatch=3
barcode-max-mismatch=1
stitching-max-mismatch=2
dedup-hd=1
threads=4
Assembly: N/A, sequencing of synthetic tags and alignment to whitelist of RTS_IDs (probe barcodes) found in the config.ini output from the GeoMx DSP platform, see attached PKC file
Supplementary files format and content: Digital Count Conversion (DCC) file format outputted from GeoMx NGS Pipeline, contains software versions, scan attributes, GeoMx NGS pipeline parameters and output metrics, Q30 scores, and list of deduplicated counts per RTS_ID (probe barcode)
Supplementary files format and content: Values represented in the collapsed target counts tab are the geometric mean of the probes for a given target--in the case that multiple probes were used-- and excludes any targets flagged as outliers. Analyzed counts represent the upper quartile normalized collapsed counts across the study after removing QC flagged segments and features.
Library strategy: GeoMx Seq

source_name: brain

extract_protocol:
Samples were incubated with DNA-oligo barcoded RNA-ISH probes which were conjugated with a UV-photocleavable linker following standard ISH protocols, along with flourescently labeled antibodies for visualization of morphological structures. Regions of interest within the tissue were illuminated with UV light and oligo barcodes were physically aspirated from the tissue and collected into microtiter plates by the GeoMx® Digital Spatial Profiler (DSP) platform. For more information about DSP protocols please see Merritt et al. Nature Biotech 2020 (doi: 10.1038/s41598-020-63539-x)
Each collection of oligo tags from one well (representing an ROI from the tissue section) was indexed with i7xi5 unique dual indexes using GeoMx SeqCode primers with 18 cycles of PCR.  After PCR, indexed AOIs were pooled and purified in two rounds of AMPure XP PCR purification using 1.2x bead:sample ratio.

value: brain

tag: slide id

tag: segment type

value: Desmin

tag: region

value: cortex

tag: replicate

tag: roi #

tag: nuclei counts


title: Cortex Desmin 14


title: Cortex Desmin 13


title: Cortex Desmin 11


title: Cortex Desmin 15


title: Cortex Desmin 10


title: Plate3_D10


title: ME Desmin 17

value: ME


title: Plate3_D20


title: ME GFP 19

value: GFP


title: Plate3_D19


title: ME Desmin 16


title: Plate3_D21


title: ME GFP 18


title: ME Desmin 15


title: Plate3_D23

value: Testosterone propionate_1_uM


title: Plate3_D22

value: iPSC-Cardiomyocytes


title: Plate3_D18

value: Dibutyl phthalate_1_uM


title: Plate3_E02


title: Cortex Desmin 9


title: ME Desmin 14


title: Plate3_E03


title: ME GFP 20


title: Plate3_D17


title: Plate3_E04


title: Plate3_E08


title: ME GFP 16


title: Cortex Desmin 8


title: ME GFP 15


title: Plate3_E05


title: Plate3_E07


title: Plate3_E12


title: ME GFP 14


title: ME Desmin 13


title: ME Desmin 12


title: ME GFP 13


title: Plate3_E10


title: Plate3_E11


title: Plate3_E13

value: Dibutyl decanedioate_0.2_uM


title: ME Desmin 11


title: ME GFP 17


title: Plate3_E16


title: ME GFP 12


title: Plate3_E14


title: ME GFP 11


title: Cortex Desmin 7


title: Plate3_E17


title: ME Desmin 9


title: Plate3_E09


title: Plate3_E06


title: Plate3_E19


title: ME GFP 10


title: Plate3_E18

value: Dibutyl phthalate_0.2_uM


title: ME Desmin 7


title: Plate3_E20


title: ME Desmin 8


title: Plate3_E21


title: ME GFP 9


title: Plate3_E22


title: ME Desmin 6


title: Plate3_F02


title: ME Desmin 5


title: ME GFP 8


title: Plate3_F03


title: ME GFP 7


title: Plate3_E23

value: Testosterone propionate_0.2_uM


title: Plate3_E15


title: ME Desmin 10


title: Plate3_F04


title: ME GFP 6


title: Plate3_F06


title: ME Desmin 4


title: Plate3_F05


title: Cortex Desmin 6


title: Plate3_F08


title: ME GFP 5


title: ME GFP 4


title: Plate3_F07


title: Plate3_F09


title: Plate2_M17


title: ME Desmin 2


title: ME Desmin 3


title: Cortex Desmin 5


title: Plate2_M18


title: Cortex Desmin 4


title: Plate2_M19


title: Plate2_M20


title: ME GFP 3


title: Plate2_M21


title: Plate2_M22


title: Plate2_N02


title: Cortex Desmin 2


title: Cotex GFP


title: ME Desmin 1


title: Plate2_N03


title: Plate2_M23


title: ME GFP 2


title: Plate2_N04


title: ME GFP 1


title: Cortex Desmin 1


title: Plate2_N06


title: Plate2_N08


title: D-xyl@epsilonPLCDs bladder

city: Tianjin

first: Chunhui

last: Miao

address: 22 Qixiangtai Road, Heping District

department: Immunology

country: China

institute: Tianjin Medical University

data_processing:
The raw paired end reads were trimmed and quality controlled by SeqPrep and Sickle with default parameters.
Then clean reads were separately aligned to reference genome with orientation mode using HISAT2 software.
The mapped reads of each sample were assembled by StringTie in a reference-based approach.
The expression level of each transcript was calculated according to the transcripts per million reads (TPM) method.
RSEM was used to quantify gene abundances.R statistical package software EdgeR (Empirical analysis of Digital Gene Expression in R, http://www.bioconductor.org/packages/2.12/bioc/html/edgeR.html) was utilized for differential expression analysis.
Assembly: GRCm39
Supplementary files format and content: Excel files include RAW counts for each sample.
Supplementary files format and content: Excel files include TPM values for each sample.

source_name: bladder

extract_protocol:
Total RNA was extracted from the tissue using TRIzol® Reagent according the manufacturer’s instructions.
RNA libraries were prepared for sequencing using standard Illumina protocols.

value: bladder

value: wild type


title: Control bladder

value: Control


title: Plate2_N07


title: Decoy6

city: Duarte

first: Haiqing

last: Li

state: CA

address: 1500 E Durate Rd

department: Shared Resources Department

institute: City of Hope National Medical Center

description: 10x Genomics

data_processing:
The demultiplexing, barcoded processing, gene counting and aggregation were made using the Cell Ranger software v3.1 (https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-ranger)
Raw sequencing data were processed using the 10x Genomics’s Cell Ranger analysis pipeline (v.7.1.0) for sample demultiplexing, barcode processing, alignment with mm10 mouse genome, filtering, and UMI counting. The scRNA-seq data were analyzed using R Seurat Package (v4). The low quality cells are further filtered by three quality control filters: >10% mitochondrial reads, <5% ribosomal reads or <200 detected features. The doublets were identified and removed using DoubletFinder. The normalization and variance stabilization was performed with SCTransform (v2) with regressing out percentage of mitochondrial reads. 2,000 most variable genes were selected to calculate the first 40 PCs for the downstream analysis. The expression profiles were clustered using Leiden algorithm and visualized using two-dimensional UMAP. The cell type of each cell cluster was identified and annotated using known maker genes. The differentially expressed genes of each cell cluster were found with pseudo-bulk method using Deseq2. The pathway enrichment analysis was done using fGSEA with Kegg, Reactome and GO BP databases.
Assembly: mm10
Supplementary files format and content: Tab-separated values files and matrix files

source_name: CMM

growth_protocol: CMM (GFP+) cells IV engrafted in C57BL/6 mice

extract_protocol:
Total RNA was extracted from sorted GFP+c-Kit+ CMM cells using Maxwell RSC simplyRNA tissue kit (Promega)
Library was performed using Single Cell-3 Solution kit v3.1 following the manufacture’s protocol. The final library size was determined using a High Sensitivity DNA Chip (Agilent). The libraries were sequenced with the paired end setting of 28 Read1 and 101 Read2 cycles, 10 index i7 and i5 cycles on NovaSeq 6000 platform (Illumina) using S4 Reagent kit v1.5 (Illumina).

treatment_protocol: start treatment when %GFP+ leukemia cells reached ~1% in blood, treat mice IV with PBS or CpG-STAT3dODN every other day for 3 times

value: AML

value: CMM


title: Plate2_N09


title: Plate2_N10


title: Decoy5

description: 10x Genomics


title: Plate2_N11

value: Diphenolic acid_0.2_uM


title: Plate2_N13


title: Cortex Desmin 3

value: brain


title: Plate2_N12


title: Decoy3

description: 10x Genomics


title: Decoy2

description: 10x Genomics


title: Plate2_N14


title: Plate2_N15