title: Ctrl-2

value: vehicle


title: RV_CHD_12

status: Public on Jul 17 2024

type: SRA

city: BRISTOL

first: Francecsa

last: Bartoli-Leonard

address: Mauldin Street

department: Bristol Medical School

country: United Kingdom

institute: University of Bristol

library_source: transcriptomic

data_processing:
FastQC (Babraham Bioinformatics, V0.12) was used to provide quality control on raw counts. Paired-end data was trimmed via SLIDINGWINDOW:4:20 command with Trimmomatic-0.3510. Quantification was conducted via Salmon11 (V1.10.2) via mapping-based mode salmon -quant indexed against human genome assembly GRCh38 (hg38).
Assembly: GRCh38
Supplementary files format and content: excel

molecule: polyA RNA

organism: Homo sapiens

source_name: snap-frozen right ventricle

extract_protocol:
Strand-specific bulk RNA sequencing was performed on whole tissue snap frozen RV biopsies via Genewiz (Azenta). Briefly, total RNA from RV was isolated from 10mg of tissue, with poly-A selection.
poly-A selection
Sequencing was conducted on Illumina NovaSeq, yielding 2x150bp paired end reads, 30 million read pairs per sample, with >80% of bases with Q30 or higher

tag: tissue

value: snap-frozen right ventricle

tag: genotype

value: tissue


title: RV_CHD_14


title: RV_CHD_13


title: Decidualized ESF cells on scar mimetic matrix , sample 2

status: Public on Jul 19 2024

city: Farmington

first: Yasir

last: Suhail

state: Connecticut

address: 263 Farmington Avenue

department: BME

country: USA

institute: UCHC

data_processing:
Alignment to the human transcriptome on Hisat2
Reads counted against genes using featureCount
Differential expression and TPM values computed using DESeq2
Assembly: GRCh38
Supplementary files format and content: TPM values

source_name: Endometrial stromal fibroblast

growth_protocol: Human isolated ESFs were maintained in phenol red free DMEM/F12 50:50 containing 25 mM glucose and supplemented with 10% charcoal stripped fetal bovine serum (Thermo Fisher), 1% antibiotic/antimycotic, and ITS (insulin, transferrin, and selenium). To induce decidualization, ESFs were treated with 0.5 mM 8-bromo-cyclic 3',5'-(hydrogen phosphate)-adenosine (Cayman Chemicals) and 1uM Medroxyprogesterone acetate (Cayman Chemicals) in DMEM/F12 medium with 2% FBS for 4 days.

extract_protocol:
Cells were lysed and RNA was isolated with RNeasy Mini Kit (Qiagen) following manufacturer’s instructions. RNA integrity was evaluated with Bioanalyzer 2100 (Agilent) and RIN ~8 was used for library preparation.
Library prep and RNA sequencing were performed by Novogene Inc. HISAT2 pipeline was used to align reads to NCBI GRCh38 genome assembly.

tag: cell type

value: Endometrial stromal fibroblast

tag: treatment

value: Decidualized ESF cells on physiological matrix


title: RV_CHD_11


title: RV_CHD_10


title: RV_CHD_8


title: RV_CHD_9


title: RV_CHD_5


title: RV_CHD_1


title: ORFTOC FTOC - HTO

status: Public on Jul 16 2024

city: Lausanne

first: Tania

last: Hübscher

address: Station 15

department: SV IBI

country: Switzerland

institute: EPFL

description: ORFTOC FTOC

library_source: transcriptomic single cell

data_processing:
The reads were aligned using Cell Ranger v6.1.2 to the mouse genome (mm10).
Raw count matrices were imported into R and analyzed using Seurat v4.2.0
HTO with less than 100 features and less than 1 count were discarded. Cells with less than 600 features, less than 0.4 or more than 10 percent mitochondrial genes were discarded.
Demultiplexing was performed using HTODemux with standard parameters.
Doublets were removed using recoverDoublets from scDblFinder package and based on doublets identified from HTOs.
Data were normalized using SCTransform and with cell cycle score as variable to regress.
The three clusters representing the main cell types were obtained using PCA and UMAP with 18 dimensions and a resolution of 0.005. Each cell type was then subset and thresholded based on EpCAM, Ptprc and Pdgfra expression.
Epithelial clusters were identified using 18 dimensions and a resolution of 0.4, leading to 7 clusters that were named based on markers from previous datasets37,38,41,42,48,59. Immune clusters were identified using 18 dimensions and a resolution of 3. Immune clusters were further merged to obtain 14 clusters
TCR analysis was conducted using scRepertoire.
Assembly: mm10
Supplementary files format and content: Tab-separated text files include count values, features and barcodes.
Supplementary files format and content: RDS objects derived from the analysis of ORFTOC FTOC - GEX and ORFTOC FTOC - HTO. Seurat object split into Epithelial and Immune cells for downstream analysis.

organism: Mus musculus

source_name: thymus

extract_protocol:
After 13 in culture, ORFTOC and FTOC samples were dissociated with TrypLE and DNase as indicated in the manuscript.
Two ORFTOC samples and two FTOC samples were pooled, respectively and for each pool 500 000 cells were labelled with TotalSeq Antibody (HTO). The single cell suspensions were filtered through a 40 μm strainer, mixed in a 1:1 ratio and processed using Chromium Next GEM Single Cell 5' Reagent Kits v2 (Dual Index) with Feature Barcode technology for Cell Surface Protein & Immune Receptor Mapping reagents following manufacturer’s instruction.

value: thymus

value: ORFTOC (HTO_2) and FTOC (HTO_1)

value: WT

value: No treatment


title: RV_CHD_3


title: RV_CHD_6


title: ORFTOC FTOC - TCR

description: ORFTOC FTOC


title: ORFTOC FTOC - GEX

description: ORFTOC FTOC


title: Decidualized ESF cells on physiological matrix, sample 3

value: Decidualized ESF cells on scar mimetic matrix


title: Ctrl-1

status: Public on Jul 18 2024

city: Duarte

first: Hsun

middle: Teresa

last: Ku

state: CA

address: 1500 E. Duarte Road

department: Translational Research and Cellular Therapeutics

institute: City of Hope

data_processing:
bcl2fastq v2.20 (loaded by cellranger mkfastq (3.0.1)) software used for basecalling.
reads were aligned to refdata-cellranger-GRCh38-3.0.0 genome assembly using cellranger v5.0.0
Counting data of reference gene expression levels were generated by cellranger v5.0.0 (automatic identification of chemistry properties)
Assembly: hg38
Supplementary files format and content: Tab separated values; MatrixMarket

molecule: total RNA

source_name: T cell

extract_protocol:
MM1S-Luc cells were intravenously (i.v.) injected to 6-10-week old NSG mice via tail vein (2x106 cells/mouse). After 5 days, tumor xenografts were confirmed by bioluminescence imaging. Mice were injected intravenously with dosed 2.5 × 105BCMA-CAR T cells followed by treatment with TAK-981 (7.5 mg/kg, intraperitoneally (i.p.) twice per week, BIW) or Vehicle (Veh) for 2 weeks. Bone marrow mononuclear cells were isolated and stained with human CD45 antibody. Total human T cells were sorted by gating GFP-/DAPI-/human CD45+.
About 1000 cells were captured per sample on a 10xGenomics Chromium controller using a 10X V3.1 Single Cell 3’ Solution kit (10xGenomics, Chromium Next GEM Single Cell 3’ Regent kits V3.1, Cat. PN-1000268). All protocols were performed following the manufacture’s instruction. Final sequencing libraries were analyzed on a High Sensitivity DNA Chip (Agilent, Cat 5067-4626) to determine the library size. The library concentration was determined with a Qubit high Sensitivity DNA assay Kit (Thermo, Cat. Q32854). The libraries were sequenced with the paired end setting of 28 cycles of read1, 101 cycles of R2, 8 cycles of index i7 and 8 cycles of index i5 read on Illumina NovaSeq 6000 platform with S4 Reagent kit v1.5 (Illumina, Cat 20028313) at TGEN with a depth of 100K - 135K reads per cell.

value: T cell


title: Kolf2-C1_Day60_biol_rep1

status: Public on Jul 08 2024

city: Cambridge

first: Davide

last: Chiarugi

address: Box 289, Addenbrooke’s Hospital

institute: Institute of Metabolic Science

data_processing:
Raw fastq files were trimmed using Cutadapt 2.10 and aligned to the reference features using STAR v. 2.7
The amount of reads per feature was quantified using Feature Counts 1.6.2 of the Subread package
Assembly: Genome-build GRCh38.p12, NCBI_Assembly:GCF_000001405.38, NCBI Homo sapiens Annotation Release 109
Supplementary files format and content: tab-delimited text files, including the raw counts for each sample

growth_protocol: Pluripotent cells were seeded onto Matrigel-coated 12-well plates for differentiation and induced at 70% confluence. The cells were then differentiated following the protocol described by Carobbio et al. (Stem Cell Rep. 16, 641–655 (2021)) until Day 60

extract_protocol:
RNA was extracted using the RNeasy Qiagen Kit following the manufacturer's instructions
Library construction, including poly(A) pulldown, fragmentation, first and second-strand synthesis, end prep, and ligation, was performed using the 'NEB Ultra II RNA custom kit' on an Agilent Bravo WS automation system. PCR setup used the KapaHiFi Hot start mix and unique dual-indexed tag barcodes on the Agilent Bravo WS automation system. Post-PCR, the plate was purified using Agencourt AMPure XP SPRI beads on the Caliper Zephyr liquid handling platform. Libraries were quantified with the Biotium Accuclear Ultra-high sensitivity dsDNA Quantitative kit using the Mosquito LV liquid handling platform, Bravo WS, and BMG FLUOstar Omega plate reader. Libraries were pooled in equimolar amounts on a Beckman BioMek NX-8 liquid handling platform, and the pooled libraries were quantified on an Agilent bioanalyser.

tag: cell line

value: Differentiated iPSC

tag: time

value: Day 60


title: Kolf2-C1_Day50_biol_rep2

value: Day 50


title: Kolf2-C1_Day50_biol_rep1


title: RV_CHD_4


title: Donor 3 medulla

status: Public on Jul 05 2024

city: San Francisco

first: Gabriel

last: Loeb

address: 555 Mission Bay Blvd South, Rm 382H

department: Medicine

institute: UCSF

description: single cell ATAC-sequencing

library_source: genomic single cell

data_processing:
Demultiplexing, read alignment, and cell calling was performed with the Cell Ranger ATAC Pipeline (v1.2, using hg38) to generate scATAC fragment files and peak barcode matrices.
Fragment files were loaded into R (3.6.1) using the createArrowFiles function in ArchR (v.0.9.5)111. TSS enrichment and number of unique fragments calculated on a per-cell basis were used to remove low quality cells, cutoffs were defined for each library (TSS enrichment >6-7, unique fragments <4000-6800). Dimensionality reduction, batch effect correction, clustering, integration with kidney scRNA-seq data (PMID: 31506348), and cluster-specific 500bp fixed-width peak calling were performed using the addIterativeLSI, addHarmony, addClusters, addGeneIntegrationMatrix, addGroupCoverages, and addReproduciblePeakSet functions in ArchR. Gene activity scores were calculated using the ArchR function getMarkerFeatures. Motif enrichment within cell type-specific peaks were identified using the addMotifAnnotations and peakAnnoEnrichment functions and Homo sapiens motifs from CIS-BP.
Bed files for the fragments from each cell type from all three donors were generated by using cellids from ArchR to extract cell type specific fragments from fragment files. Cell type-specific bigwig files were generated from these bed files using MACS2 callpeak with options: -f BED -g 2.7e9 --keep-dup all -B --SPMR --shift -75 --extsize 150 -q 0.05 --nomodel --nolambda.
To identify clusters of cell type-specific peaks, the cell type-by-peak matrix was normalized, log transformed, and k-means clustered with 12 clusters. Two clusters corresponded to ubiquitously accessible peaks  were combined for a total of 11 clusters.
Assembly: hg38
Supplementary files format and content: Peak barcode matrices were generated using the Cell Ranger ATAC Pipeline (v1.2, using hg38 genome assembly)

molecule: genomic DNA

source_name: Kidney medulla

extract_protocol:
Nuclei were isolated from 2g of kidney cortex or medulla, homogenized in nuclei isolation buffer (250mM sucrose, 10mM Tris pH 7.5, 3mM MgCl2, 0.1% NP40) by gentleMacs using dissociator proram E.01 and filtered through a 200uM filter. Homogenate was resuspended in 1ml of ATAC-Resuspension Buffer with detergents (10mM Tris pH 7.5, 10mM NaCl, 3mM MgCl2, 100ug/mL digitonin, 0.1% Tween, 0.1% NP40) for 3 minutes on ice and then detergents were diluted by resuspending with 14mL ATAC-Resuspension Buffer with tween alone (10mM Tris pH 7.5, 10mM NaCl, 3mM MgCl2, 0.1% Tween). Nuclei were passed through a 40mm filter, counted, and 15,000 nuclei were used for transposition and library generation using the Chromium Next GEM Single Cell ATAC Library & Gel Bead Kit v1.1 (10x Genomics, 1000176).
Chromium Next GEM Single Cell ATAC Library & Gel Bead Kit v1.1 with 15,000 nuclei according the manufacturer's protocol.

value: Kidney medulla


title: Donor 2 medulla

description: single cell ATAC-sequencing


title: Donor 2 cortex

description: single cell ATAC-sequencing

source_name: Kidney cortex

value: Kidney cortex


title: Donor 1 cortex

description: single cell ATAC-sequencing


title: Shrimp allergic patient, with tropomyosin stimulation, peripheral blood mononuclear cells

status: Public on Jul 01 2024

city: Houston

first: Hongjie

last: Li

state: Texas

address: N910 Alkek Building

department: Huffington Center on Aging

institute: Baylor College of Medicine

description: 10X Genomics 5v2

data_processing:
Demultiplexing, barcode assignment, cell filtering, gene counting, and aggregating performed with CellRanger v6.1.2.
Ambient RNA removal, doublet removal, and low quality cell filtering performed using Seurat v4.3.0
Assembly: GRCh38
Supplementary files format and content: CellRanger output filtered matrix files and downstream merged sample processed data matrix (.rds format)

source_name: Peripheral blood

extract_protocol:
Collect PBMCs and serum from the patient. Centrifuge the solution down at 1100g for 10 minutes. Then the blood was specifically isiolated from the tube and transferred to a new tube. Centrifugation at various speeds was performed again to isolate the PBMCs from the serum layer. The PBMCs were counted and viability was assessed using an automated counter. If the viability was >95%, the PBMCs were loaded at a concentration of 10,000 cells into the 10X Genomics 5' Sequencing Chromium Chip.
Used the 10X Genomics 5v2 library construction kit. PBMCs from unstimulated and stimulated healthy control and shrimp allergic pateints were resuspended in the master mix and loaded with the commercial provider's partitioning oil and gel beads to generate the gel in bead emulsion (GEMs) using the 10X genomics Chromium Controller. The poly-A RNA were retrotranscribed to cDNA to incorporate the Illumina R1 sequence (read 1 sequencing primer), the 16 nt 10x Barcode, the 10 nt unique molecular identifier, and the 13 nt template switch oligo. The pooled barcoded cDNA was cleaned using the Silane DynaBeads, amplified with PCR, and the appropriately sized fragments were selected with the SPRIselect reagent for downstream library construction steps. During these library construction steps the P5, P7, i5 and i7 sample indices as well as the Illumina R2 sequence (read 2 primer sequence) were added.

value: Peripheral blood

value: Peripheral blood mononuclear cells

value: SA-stim

value: Tropomyosin, 1 ug.mL


title: Shrimp allergic patient, without tropomyosin stimulation, peripheral blood mononuclear cells

description: 10X Genomics 5v2

value: SA-unstim


title: CD4, scRNA-seq group 6

city: New Haven

first: Ya-Chi

last: Ho

address: 295 Congress Avenue,BCMM 354E

institute: Yale University

data_processing:
Raw reads were processed using the Cellranger arc pipeline (version 2.0.1) with default parameters, CITE reads were processed with Kite (https://github.com/pachterlab/kite) and Kallisto (version 0.48.0).
Assembly: hg38
Supplementary files format and content: Matrix files and fragments.tsv files from the cellranger pipeline or Kallisto
Supplementary files format and content: Morphine.Meta.csv contains hashkey, treatment conditions, and timepoint keys
Library strategy: DOGMA-seq

source_name: Peripheral Blood

extract_protocol:
cDNA, transposed cDNA, and CITE cDNA were extracted according to the 10X multiome protocol with the modifications described in Mimitou et al.Nat. Biotechnol. 2021.
Libraries were constructed using the 10X multiome protocol with the modificaitons described in Mimitou et al. Nat. Biotechnol. 2021.

treatment_protocol: Dead cells were removed using a bead based kit (STEMCELL Technologies #17899), and hashed using TotalSeq-A antibodies. Each sample was pooled in equal ratios and stained with the TotalSeq™-A Human Universal Cocktail, V1.0 (Bioledgend #399907). Pools were then lysed on ice with DIG lysis buffer for 5 minutes before washing. Cells werethen loaded into the 10X Chromium controller (10x Genomics) according to the manufacturer protocol. CD4+ T cells samples were additionally negatively selected using Miltenyi Biotech #130-096-533.

value: Peripheral Blood


title: CD4, scRNA-seq group 3


title: PBMC, scRNA-seq group 3

value: PBMC


title: CD4, scRNA-seq group 1


title: CD4, scRNA-seq group 4


title: PBMC, scRNA-seq group 2


title: Kolf2-C1_Day60_biol_rep2


title: CD4, scRNA-seq group 5


title: Kolf2-C1_Day60_biol_rep3


title: PBMC, scRNA-seq group 1


title: CD4, scRNA-seq group 2


title: 20-mo CD45- thymic stoma cells - wild type - male [singe cell]

status: Public on Jun 27 2024

first: Anastasia

middle: I

last: Kousa

address: 1500 E Duarte Rd

department: Hematology / HCT

data_processing:
FASTQ files were processed using the 10x Cell Ranger package (v7.01).
Assembly: GRCm38.p5 (mm10) + tdTomato-WPRE
Supplementary files format and content: Foxn1Lin_annotated.h5ad: H5AD file with raw and normalized read counts and cell annotation (after bad quality cell filtering)
Supplementary files format and content: filtered_feature_bc_matrix files (barcodes, features, matrix): contain only detected cell-associated barcodes in MEX format; each element of the matrix is the number of UMIs associated with a feature (row) and a barcode (column)
Supplementary files format and content: filtered_feature_bc_matrix.h5: same information as filtered_feature_bc_matrix in HDF5 format
Supplementary files format and content: raw_feature_bc_matrix files (barcodes, features, matrix): contain all detected barcodes in MEX format; each element of the matrix is the number of UMIs associated with a feature (row) and a barcode (column)
Supplementary files format and content: raw_feature_bc_matrix.h5: same information as raw_feature_bc_matrix in HDF5 format
Supplementary files format and content: metrics_summary.csv: metrics for quality assessment in CSV format
Supplementary files format and content: molecule_info.h5: contains per-molecule information for all molecules that contain a valid barcode, valid UMI, and were assigned with high confidence to a gene or Feature Barcode; this file is a required input to run cellranger aggr

extract_protocol:
All steps were performed at 4 C unless indicated. 10-15 thymi of 20-month-old female C57BL/6 mice were excised and enzymatically digested. Briefly, thymi were mechanically dissociated into ca. 2 mm pieces. Tissue pieces were incubated with a digestion buffer (RPMI, 10% FCS, 62.5 um/mL liberase TM, 0.4 mg/ml DNase I) twice for 30 min at 37 C. Between incubation steps, supernatant containing dissociated cells was transferred to 50 mL conical tubes equipped with 100 um filter. Cells were pelleted by centrifugation at 400g for 5 min. Cell pellets were incubated with anti-mouse CD45 microbeads and CD45-positive cells were depleted from cell suspension using magnetic-associated cell sorting (MACS) on LS columns according to manufacturer’s protocol. Following red blood cell lysis using ACK buffer, the CD45-depleted cell fraction was incubated with an antibody cocktail for 15 min at 4 C and cells of interest were purified by fluorescent-associated cell sorting (FACS) on a BD Biosciences Aria II using a 100 um nozzle. Cells were sorted into tubes containing RPMI supplemented with 2% BSA. FACS-purified cells were spun down at 400g for 5 min and resuspended in PBS supplemented with 0.04 % BSA for generation of single-cell suspensions.
The single-cell RNA-seq of FACS-sorted cell suspensions was performed on Chromium instrument (10X genomics) following the user guide manual (CG00052 Rev E) and using Single Cell 3’ Reagent Kit (v2). The viability of cells prior to loading onto the encapsulation chip was 73-98%, as confirmed with 0.2% (w/v) Trypan Blue stain. Each sample, containing approximately 8000 cells, was encapsulated in microfluidic droplets at a final dilution of 66–70 cells/µl (a multiplet rate ~3.9%). Following reverse transcription step the emulsion droplets were broken, barcoded-cDNA purified with DynaBeads and amplified by 12-cycles of PCR: 98 ̊C for 180 s, 12x (98 ̊C for 15 s, 67 ̊ C for 20 s, 72 ̊C for 60 s), and 72 ̊C for 60 s. The 50 ng of PCR-amplified barcoded-cDNA was fragmented with the reagents provided in the kit, purified with SPRI beads and resulting DNA library was ligated to the sequencing adapter followed by indexing PCR: 98 ̊C for 45 s; 12x 98 ̊C for 20 s, 54 ̊C for 30 s, 72 ̊C for 20 s), and 72 ̊C for 60 s. The final DNA library was double-size purified (0.6–0.8X) with SPRI beads and sequenced on Illumina Nova-Seq platform (R1 – 26 cycles, i7 – 8 cycles, R2 – 70 cycles or higher) at depth of 200-270 million reads per sample.

value: thymus

tag: strain

value: CD45-PECAM1+ thymic stroma cells

value: wild type

tag: age

value: 20-month old

value: untreated

tag: Sex

value: male


title: Kolf2-C1_Day50_biol_rep3

value: Differentiated iPSC

value: WT


title: 20-mo CD45- thymic stoma cells - Foxn1Lin - female [singe cell]

value: CD45- thymic stroma cells

value: Foxn1 lineage trace

value: female


title: Donor 3 cortex

description: single cell ATAC-sequencing

value: Kidney cortex


title: HA_control ChIP-Seq

status: Public on Jun 19 2024

city: shanghai

first: Zhenyu

last: Shao

state: --- Select a state ---

address: Yueyang Road 320

department: Center for Excellence in Molecular Cell Science

country: China

institute: Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences

library_source: genomic

data_processing:
For ChIP seq Data analysis, raw data were trimmed by Trim Galore with default settings. Then, the clean data were mapped to mouse reference genome mm10 using bowtie2 with the parameters --no-unal --no-mixed --no-discordant -X 2000. Duplicates were removed using Picard. Metaplot and heatmap were generated using deepTools. Peaks were called using MACS2 callpeak and annotated with ChIPseeker package. Those overlapped with promoters were further subject to STREME for motif discovery.
Assembly: mm10
Supplementary files format and content: bigwig files for Tdg and Trp53 enrichment

source_name: cell line

growth_protocol: B16 cells were maintained in DMEM supplemented with 10% fetal bovine serum (FBS) and antibiotics in humidified atmosphere with 5% CO2 at 37°C

extract_protocol:
For ChIP-seq, cells were crosslinked with 1% formaldehyde for 10 min at room temperature and quenched with 0.125 M glycine. The final DNA length was approximately 300 bp sonicated using a COVARIS S220.
For IP, 1/10 of the supernatant was incubated with the HA (CST, 3724) or p53 (CST, 2524) antibody at 4 °C overnight and immunoprecipitation was performed with Protein G magnetic beads. Immunoprecipitated chromatin (ChIP DNA) was eluted in TE buffer. Following end-repair, A-tailing, adaptor ligation, library amplification was performed with the VAHTS Universal DNA Library Prep Kit for Illumina kit (Vazyme, ND607).

value: cell line

value: Melanoma

tag: chip antibody


title: IgG ChIP-Seq

value: Tdg HA knockin

value: Rabbit IgG (Abclone, ac005)


title: HA ChIP-Seq


title: p53KO-p53 ChIP-Seq

value: Trp53 KO


title: p53 ChIP-Seq


title: input ChIP-Seq


title: IgG

data_processing: For ChIP seq Data analysis, raw data were trimmed by Trim Galore with default settings. Then, the clean data were mapped to mouse reference genome mm10 using bowtie2 with the parameters --no-unal --no-mixed --no-discordant -X 2000. Duplicates were removed using Picard. Metaplot and heatmap were generated using deepTools. Peaks were called using MACS2 callpeak and annotated with ChIPseeker package. Those overlapped with promoters were further subject to STREME for motif discovery.

growth_protocol: NCI-H1299 cells were maintained in 1640 supplemented with 10% fetal bovine serum (FBS) and antibiotics in humidified atmosphere with 5% CO2 at 37°C.

extract_protocol:
For ChIP-seq, cells were crosslinked with 1% formaldehyde for 10 min at room temperature and quenched with 0.125 M glycine. The final DNA length was approximately 300 bp sonicated using a COVARIS S220.
For IP, 1/10 of the supernatant was incubated with the FLAG or IgG antibody at 4 °C overnight and immunoprecipitation was performed with Protein G magnetic beads. Immunoprecipitated chromatin (ChIP DNA) was eluted in TE buffer. Following end-repair, A-tailing, adaptor ligation, library amplification was performed with the VAHTS Universal DNA Library Prep Kit for Illumina kit (Vazyme, ND607).

treatment_protocol: Cells were treated with C-271 (5 μM) or DMSO for 48-hour.

value: NSCLC

value: FLAG-TDG expressed

value: Mouse IgG (Abcam, ab97019)


title: Healthy control patient, without tropomyosin stimulation, peripheral blood mononuclear cells

description: 10X Genomics 5v2

value: Peripheral blood

value: Peripheral blood mononuclear cells

value: HC-unstim


title: Healthy control patient, with tropomyosin stimulation, peripheral blood mononuclear cells

description: 10X Genomics 5v2

value: HC-stim

value: Tropomyosin, 1 ug.mL


title: C271

value: FLAG (Sigma, F1804)


title: DMSO


title: BLISS_shNT_OHT_rep2

city: Milano

first: Stefano

last: Campaner

address: Via adamello 16

department: Center for Genomic Science

country: Italy

institute: fondazione Istituto italiano di tecnologia

data_processing:
Data were analysed according to the sBLISS-seq protocol (Bouwman, B.A.M., Agostini, F., Garnerone, S. et al. Genome-wide detection of DNA double-strand breaks by in-suspension BLISS. Nat Protoc 15, 3894–3941 (2020). https://doi.org/10.1038/s41596-020-0397-2)
Assembly: hg19
Library strategy: SBLISS-seq

extract_protocol:
Cells were fixed with 2%PFA and processed according to the publised sBLISS protocol (Bouwman, B.A.M., Agostini, F., Garnerone, S. et al. Genome-wide detection of DNA double-strand breaks by in-suspension BLISS. Nat Protoc 15, 3894–3941 (2020). https://doi.org/10.1038/s41596-020-0397-2)
Libraries were prepared according to the published sBLISS-seq protocol

value: shNT OHT


title: 20-mo CD45- thymic stoma cells - Foxn1Lin - male [singe cell]

value: thymus

value: CD45- thymic stroma cells

value: Foxn1 lineage trace

value: 20-month old

value: untreated

value: male


title: BLISS_shNT_OHT_rep1


title: Infarcted heart Tregs replicate 3

status: Public on May 31 2024

city: Clayton

first: Mikaël

last: Martino

state: Victoria

address: 15 Innovation Walk

department: Australian Regenerative Medicine Institute

country: Australia

institute: Monash University

data_processing:
Raw fastq files were analysed using the RNAsik pipeline (Tsyganov, K., et al., 2018, Journal of Open Source Software) using STAR aligner (Dobin, A., et al., 2013, Bioinformatics) with Mus musculus GRCm38.
Reads were quantified using featureCounts (Liao, Y., et al., 2014, Bioinformatics) producing the raw genes count matrix and various quality control metrics.
Raw counts (forward stranded reads) were then analysed with Degust web tool (Powell, D.R., et al., 2019, Zenodo) which performs normalisation using trimmed mean of M values (TMM) (Robinson, M.D. and Oshlack, A., 2010, Genome Biol) and differential gene expression analysis using limma/voom (Law, C.W., et al., 2014, Genome Biol)
Samples were batched corrected to account for variation between two different sequencing facilities across replicates. This was done within Degust by adding a covariate to the linear model for each sequencing batch which reduced the noise and improved the power of the test.
Assembly: GRCm38
Supplementary files format and content: csv, gene counts, rows represent genes and each column represents different information, starting from the chromosome, gene name, biotype, followed by read counts corresponding to the individual samples.

source_name: heart

extract_protocol:
RNA was extracted using the RNeasy Plus Micro Kit (Qiagen, #74034) according to the manufacturer’s instructions
Libraries were prepared with the Tecan/Nugen Ovation Solo Library prep kit, starting with 1 ng of input total RNA

value: heart

value: Regulatory T cells

value: 10 weeks

value: myocardial infarction (MI)

value: day 7 post-MI


title: FFPE archived fetal lung tissue GW18; Xenium

status: Public on Jun 04 2024

type: RNA

city: Toronto

first: Amy

last: Wong

state: Ontario

address: 686 Bay Street, Toronto

department: Developmental & Stem Cell Biology

country: Canada

institute: The Hospital for Sick Children

hyb_protocol: A total of 339 unique genes were included in the analysis of this dataset. 289 genes came from the pre-designed  human lung base panel and 50 genes from a custom designed panel (CFVU2E). Stepwise Xenium FFPE preparation guidelines, buffer recipes, and procol was done according to manufacturer's instructions (10X Genomics Demonstrated Protocol CG000578).

scan_protocol: Scan protocol was done as per manufacturer's instruction (10X Genomics’ Demonstrated Protocol CG000582)

data_processing: Raw data underwent Xenium's Onboard Analysis (https://www.10xgenomics.com/support/software/xenium-onboard-analysis/latest).

label: Gene targeting probes

source_name: FFPE archived fetal lung tissue GW18

label_protocol: Deparaffinization and decrosslinking was performed and labelled as per 10X Genomics Demonstrated Protocol CG000580.

extract_protocol: Sectioning was performed at 5um and placed on Xenium slides (10X Genomics) and left to dry overnight as per manufacturer's instructions. Slides were then stored in a sealed dessicator at room temperature for maximum 10 days.

treatment_protocol: All protocol workstations and equipment were cleaned using RNase wiper to prevent RNase and DNA contamination

value: fetal lung tissue

tag: developmental stage

value: GW 18


title: Uninjured/ healthy spleen Tregs replicate 5

description:
HS_Tregs_2
Processed_Endo_injured_BMS_and_healthy_Spleen_data.csv

source_name: spleen

value: spleen

value: control (uninjured/ healthy)


title: FFPE archived fetal lung tissue GW15; Xenium

source_name: FFPE archived fetal lung tissue GW15

value: GW 15


title: Uninjured/ healthy spleen Tregs replicate 4

description:
HS_Tregs_1
Processed_Endo_injured_BMS_and_healthy_Spleen_data.csv


title: Uninjured/ healthy spleen Tregs replicate 6

description:
HS_Tregs_3
Processed_Endo_injured_BMS_and_healthy_Spleen_data.csv


title: Uninjured/ healthy spleen Tregs replicate 1

description:
Endo-HS1
Processed_Endo_injured_BMS_and_healthy_Spleen_data.csv


title: Uninjured/ healthy spleen Tregs replicate 2

description:
Endo-HS2
Processed_Endo_injured_BMS_and_healthy_Spleen_data.csv


title: Uninjured/ healthy spleen Tregs replicate 3

description:
Endo-HS3
Processed_Endo_injured_BMS_and_healthy_Spleen_data.csv


title: Infarcted heart Tregs replicate 2


title: Genomic DNA from Blood from subject BoA50

status: Public on May 29 2024

type: genomic

city: Boston

first: Jesse

last: Poganik

state: MA

address: 77 Avenue Louis Pasteur

department: Medicine/Genetics

institute: Brigham and Women's Hospital, Harvard Medical School

hyb_protocol: Standard Illumina protocol

scan_protocol: Standard Illumina protocol

data_processing:
Raw idat methylation files were processed using the Sesame R package (Zhou W et al., NAR 2018). Processing was done with the openSesame function with the reccommended prep code for human samples profiled by EPICv2 ("QCDPC").
Probe quality masked, dye bias corrected, p-value masked (oob), background subtracted (oob) beta values

label: cy3, cy5

source_name: Blood from subject BoA50

label_protocol: Standard Illumina protocol

extract_protocol: Genomic DNA isolated from human blood was obtained from the MGB (Mass General Brigham) Biobank.

tag: subject id

value: blood

value: F

value: White

tag: ethnicity

value: Non Hispanic


title: Genomic DNA from Blood from subject BoA52

source_name: Blood from subject BoA52


title: Genomic DNA from Blood from subject BoA51

source_name: Blood from subject BoA51

value: M


title: Genomic DNA from Blood from subject BoA53

source_name: Blood from subject BoA53

value: American Indian or Alaska Native

value: Black


title: Genomic DNA from Blood from subject BoA48

source_name: Blood from subject BoA48

value: Unknown/Missing


title: Genomic DNA from Blood from subject BoA54

source_name: Blood from subject BoA54

value: Other


title: Genomic DNA from Blood from subject BoA55

source_name: Blood from subject BoA55


title: Genomic DNA from Blood from subject BoA45

source_name: Blood from subject BoA45


title: Genomic DNA from Blood from subject BoA44

source_name: Blood from subject BoA44


title: Genomic DNA from Blood from subject BoA43

source_name: Blood from subject BoA43


title: Genomic DNA from Blood from subject BoA42

source_name: Blood from subject BoA42


title: Infarcted heart Tregs replicate 1

value: heart

value: Regulatory T cells

value: male

value: 10 weeks


title: Genomic DNA from Blood from subject BoA41

source_name: Blood from subject BoA41


title: Genomic DNA from Blood from subject BoA47

source_name: Blood from subject BoA47


title: Genomic DNA from Blood from subject BoA40

source_name: Blood from subject BoA40

value: Asian


title: Genomic DNA from Blood from subject BoA39

source_name: Blood from subject BoA39


title: Genomic DNA from Blood from subject BoA38

source_name: Blood from subject BoA38


title: Genomic DNA from Blood from subject BoA36

source_name: Blood from subject BoA36

value: HISPANIC


title: Genomic DNA from Blood from subject BoA34

source_name: Blood from subject BoA34


title: Genomic DNA from Blood from subject BoA31

source_name: Blood from subject BoA31


title: Genomic DNA from Blood from subject BoA49

source_name: Blood from subject BoA49


title: Genomic DNA from Blood from subject BoA30

source_name: Blood from subject BoA30


title: Genomic DNA from Blood from subject BoA33

source_name: Blood from subject BoA33


title: Genomic DNA from Blood from subject BoA32

source_name: Blood from subject BoA32


title: Genomic DNA from Blood from subject BoA28

source_name: Blood from subject BoA28


title: Genomic DNA from Blood from subject BoA37

source_name: Blood from subject BoA37


title: Genomic DNA from Blood from subject BoA46

source_name: Blood from subject BoA46


title: Genomic DNA from Blood from subject BoA27

source_name: Blood from subject BoA27


title: Genomic DNA from Blood from subject BoA19

source_name: Blood from subject BoA19


title: Genomic DNA from Blood from subject BoA35

source_name: Blood from subject BoA35


title: Genomic DNA from Blood from subject BoA24

source_name: Blood from subject BoA24


title: Genomic DNA from Blood from subject BoA25

source_name: Blood from subject BoA25


title: Genomic DNA from Blood from subject BoA22

source_name: Blood from subject BoA22


title: Genomic DNA from Blood from subject BoA23

source_name: Blood from subject BoA23


title: Genomic DNA from Blood from subject BoA20

source_name: Blood from subject BoA20


title: Genomic DNA from Blood from subject BoA18

source_name: Blood from subject BoA18


title: Genomic DNA from Blood from subject BoA26

source_name: Blood from subject BoA26


title: Genomic DNA from Blood from subject BoA29

source_name: Blood from subject BoA29


title: Genomic DNA from Blood from subject BoA17

source_name: Blood from subject BoA17


title: Genomic DNA from Blood from subject BoA16

source_name: Blood from subject BoA16


title: Genomic DNA from Blood from subject BoA15

source_name: Blood from subject BoA15


title: Genomic DNA from Blood from subject BoA14

source_name: Blood from subject BoA14


title: Genomic DNA from Blood from subject BoA11

source_name: Blood from subject BoA11


title: Genomic DNA from Blood from subject BoA12

source_name: Blood from subject BoA12


title: Genomic DNA from Blood from subject BoA9

source_name: Blood from subject BoA9


title: Genomic DNA from Blood from subject BoA8

source_name: Blood from subject BoA8


title: Genomic DNA from Blood from subject BoA10

source_name: Blood from subject BoA10


title: Genomic DNA from Blood from subject BoA6

source_name: Blood from subject BoA6


title: Genomic DNA from Blood from subject BoA7

source_name: Blood from subject BoA7


title: Genomic DNA from Blood from subject BoA3

source_name: Blood from subject BoA3


title: Genomic DNA from Blood from subject BoA2

source_name: Blood from subject BoA2


title: Genomic DNA from Blood from subject BoA5

source_name: Blood from subject BoA5


title: Genomic DNA from Blood from subject BoA21

source_name: Blood from subject BoA21


title: Genomic DNA from Blood from subject BoA500

source_name: Blood from subject BoA500


title: Genomic DNA from Blood from subject BoA4

source_name: Blood from subject BoA4


title: Genomic DNA from Blood from subject BoA495

source_name: Blood from subject BoA495


title: Genomic DNA from Blood from subject BoA493

source_name: Blood from subject BoA493


title: Genomic DNA from Blood from subject BoA497

source_name: Blood from subject BoA497


title: Genomic DNA from Blood from subject BoA496

source_name: Blood from subject BoA496


title: Genomic DNA from Blood from subject BoA1

source_name: Blood from subject BoA1


title: Genomic DNA from Blood from subject BoA494

source_name: Blood from subject BoA494


title: Genomic DNA from Blood from subject BoA491

source_name: Blood from subject BoA491


title: Genomic DNA from Blood from subject BoA499

source_name: Blood from subject BoA499


title: Genomic DNA from Blood from subject BoA498

source_name: Blood from subject BoA498


title: Genomic DNA from Blood from subject BoA490

source_name: Blood from subject BoA490


title: Genomic DNA from Blood from subject BoA13

source_name: Blood from subject BoA13


title: Genomic DNA from Blood from subject BoA492

source_name: Blood from subject BoA492


title: Genomic DNA from Blood from subject BoA487

source_name: Blood from subject BoA487


title: Genomic DNA from Blood from subject BoA489

source_name: Blood from subject BoA489


title: Genomic DNA from Blood from subject BoA486

source_name: Blood from subject BoA486


title: Genomic DNA from Blood from subject BoA485

source_name: Blood from subject BoA485


title: Genomic DNA from Blood from subject BoA484

source_name: Blood from subject BoA484


title: Genomic DNA from Blood from subject BoA482

source_name: Blood from subject BoA482


title: Genomic DNA from Blood from subject BoA481

source_name: Blood from subject BoA481


title: Genomic DNA from Blood from subject BoA479

source_name: Blood from subject BoA479


title: Genomic DNA from Blood from subject BoA475

source_name: Blood from subject BoA475


title: Genomic DNA from Blood from subject BoA478

source_name: Blood from subject BoA478


title: Genomic DNA from Blood from subject BoA477

source_name: Blood from subject BoA477


title: Genomic DNA from Blood from subject BoA474

source_name: Blood from subject BoA474


title: Genomic DNA from Blood from subject BoA476

source_name: Blood from subject BoA476


title: Genomic DNA from Blood from subject BoA472

source_name: Blood from subject BoA472


title: Genomic DNA from Blood from subject BoA488

source_name: Blood from subject BoA488


title: Genomic DNA from Blood from subject BoA467

source_name: Blood from subject BoA467


title: Genomic DNA from Blood from subject BoA473

source_name: Blood from subject BoA473


title: Genomic DNA from Blood from subject BoA468

source_name: Blood from subject BoA468


title: Genomic DNA from Blood from subject BoA466

source_name: Blood from subject BoA466

value: DECLINED


title: Genomic DNA from Blood from subject BoA471

source_name: Blood from subject BoA471


title: Genomic DNA from Blood from subject BoA463

source_name: Blood from subject BoA463


title: Genomic DNA from Blood from subject BoA470

source_name: Blood from subject BoA470


title: Genomic DNA from Blood from subject BoA465

source_name: Blood from subject BoA465


title: Genomic DNA from Blood from subject BoA461

source_name: Blood from subject BoA461


title: TAK-1

source_name: T cell

value: T cell

value: TAK-981 7.5mg/kg, twice per week, 2 weeks


title: Genomic DNA from Blood from subject BoA464

source_name: Blood from subject BoA464

value: blood


title: Genomic DNA from Blood from subject BoA462

source_name: Blood from subject BoA462

value: White


title: Genomic DNA from Blood from subject BoA460

source_name: Blood from subject BoA460

value: Non Hispanic


title: Genomic DNA from Blood from subject BoA458

source_name: Blood from subject BoA458

value: M


title: Genomic DNA from Blood from subject BoA459

source_name: Blood from subject BoA459

value: F


title: Genomic DNA from Blood from subject BoA469

source_name: Blood from subject BoA469

value: Black


title: Genomic DNA from Blood from subject BoA456

source_name: Blood from subject BoA456


title: Genomic DNA from Blood from subject BoA483

source_name: Blood from subject BoA483


title: Genomic DNA from Blood from subject BoA480

source_name: Blood from subject BoA480


title: Genomic DNA from Blood from subject BoA454

source_name: Blood from subject BoA454


title: Genomic DNA from Blood from subject BoA450

source_name: Blood from subject BoA450


title: Genomic DNA from Blood from subject BoA449

source_name: Blood from subject BoA449


title: Genomic DNA from Blood from subject BoA442

source_name: Blood from subject BoA442


title: Genomic DNA from Blood from subject BoA440

source_name: Blood from subject BoA440


title: Genomic DNA from Blood from subject BoA453

source_name: Blood from subject BoA453


title: Genomic DNA from Blood from subject BoA444

source_name: Blood from subject BoA444


title: Genomic DNA from Blood from subject BoA448

source_name: Blood from subject BoA448


title: Genomic DNA from Blood from subject BoA457

source_name: Blood from subject BoA457


title: Genomic DNA from Blood from subject BoA447

source_name: Blood from subject BoA447


title: Genomic DNA from Blood from subject BoA434

source_name: Blood from subject BoA434


title: Genomic DNA from Blood from subject BoA433

source_name: Blood from subject BoA433


title: Genomic DNA from Blood from subject BoA432

source_name: Blood from subject BoA432


title: Genomic DNA from Blood from subject BoA437

source_name: Blood from subject BoA437


title: Genomic DNA from Blood from subject BoA452

source_name: Blood from subject BoA452


title: Genomic DNA from Blood from subject BoA430

source_name: Blood from subject BoA430


title: Genomic DNA from Blood from subject BoA439

source_name: Blood from subject BoA439


title: Genomic DNA from Blood from subject BoA441

source_name: Blood from subject BoA441


title: Genomic DNA from Blood from subject BoA431

source_name: Blood from subject BoA431


title: Genomic DNA from Blood from subject BoA443

source_name: Blood from subject BoA443


title: Genomic DNA from Blood from subject BoA451

source_name: Blood from subject BoA451


title: Genomic DNA from Blood from subject BoA455

source_name: Blood from subject BoA455


title: Genomic DNA from Blood from subject BoA428

source_name: Blood from subject BoA428


title: Genomic DNA from Blood from subject BoA446

source_name: Blood from subject BoA446


title: Genomic DNA from Blood from subject BoA438

source_name: Blood from subject BoA438


title: Genomic DNA from Blood from subject BoA445

source_name: Blood from subject BoA445


title: Genomic DNA from Blood from subject BoA435

source_name: Blood from subject BoA435

value: Unknown/Missing


title: Genomic DNA from Blood from subject BoA436

source_name: Blood from subject BoA436


title: Genomic DNA from Blood from subject BoA427

source_name: Blood from subject BoA427


title: Genomic DNA from Blood from subject BoA429

source_name: Blood from subject BoA429


title: Genomic DNA from Blood from subject BoA426

source_name: Blood from subject BoA426


title: Genomic DNA from Blood from subject BoA424

source_name: Blood from subject BoA424


title: Genomic DNA from Blood from subject BoA422

source_name: Blood from subject BoA422


title: Genomic DNA from Blood from subject BoA421

source_name: Blood from subject BoA421


title: Genomic DNA from Blood from subject BoA418

source_name: Blood from subject BoA418


title: Genomic DNA from Blood from subject BoA412

source_name: Blood from subject BoA412


title: Genomic DNA from Blood from subject BoA417

source_name: Blood from subject BoA417


title: Genomic DNA from Blood from subject BoA411

source_name: Blood from subject BoA411


title: Genomic DNA from Blood from subject BoA413

source_name: Blood from subject BoA413


title: Genomic DNA from Blood from subject BoA419

source_name: Blood from subject BoA419

value: Other


title: Genomic DNA from Blood from subject BoA423

source_name: Blood from subject BoA423


title: Genomic DNA from Blood from subject BoA410

source_name: Blood from subject BoA410


title: Genomic DNA from Blood from subject BoA409

source_name: Blood from subject BoA409


title: Genomic DNA from Blood from subject BoA416

source_name: Blood from subject BoA416


title: Genomic DNA from Blood from subject BoA406

source_name: Blood from subject BoA406


title: Genomic DNA from Blood from subject BoA405

source_name: Blood from subject BoA405


title: Genomic DNA from Blood from subject BoA402

source_name: Blood from subject BoA402


title: Genomic DNA from Blood from subject BoA403

source_name: Blood from subject BoA403


title: Genomic DNA from Blood from subject BoA401

source_name: Blood from subject BoA401


title: Genomic DNA from Blood from subject BoA407

source_name: Blood from subject BoA407


title: Genomic DNA from Blood from subject BoA404

source_name: Blood from subject BoA404


title: Genomic DNA from Blood from subject BoA399

source_name: Blood from subject BoA399


title: Genomic DNA from Blood from subject BoA398

source_name: Blood from subject BoA398


title: Genomic DNA from Blood from subject BoA397

source_name: Blood from subject BoA397


title: Genomic DNA from Blood from subject BoA425

source_name: Blood from subject BoA425


title: Genomic DNA from Blood from subject BoA396

source_name: Blood from subject BoA396


title: Genomic DNA from Blood from subject BoA395

source_name: Blood from subject BoA395


title: Genomic DNA from Blood from subject BoA420

source_name: Blood from subject BoA420


title: Genomic DNA from Blood from subject BoA414

source_name: Blood from subject BoA414


title: Genomic DNA from Blood from subject BoA394

source_name: Blood from subject BoA394


title: Genomic DNA from Blood from subject BoA415

source_name: Blood from subject BoA415


title: Genomic DNA from Blood from subject BoA393

source_name: Blood from subject BoA393


title: Genomic DNA from Blood from subject BoA408

source_name: Blood from subject BoA408


title: Genomic DNA from Blood from subject BoA391

source_name: Blood from subject BoA391


title: Genomic DNA from Blood from subject BoA392

source_name: Blood from subject BoA392


title: Genomic DNA from Blood from subject BoA390

source_name: Blood from subject BoA390


title: Genomic DNA from Blood from subject BoA387

source_name: Blood from subject BoA387


title: Genomic DNA from Blood from subject BoA389

source_name: Blood from subject BoA389


title: Genomic DNA from Blood from subject BoA400

source_name: Blood from subject BoA400


title: Genomic DNA from Blood from subject BoA386

source_name: Blood from subject BoA386


title: Genomic DNA from Blood from subject BoA388

source_name: Blood from subject BoA388


title: Genomic DNA from Blood from subject BoA383

source_name: Blood from subject BoA383


title: Genomic DNA from Blood from subject BoA382

source_name: Blood from subject BoA382


title: Genomic DNA from Blood from subject BoA385

source_name: Blood from subject BoA385


title: Genomic DNA from Blood from subject BoA380

source_name: Blood from subject BoA380


title: Genomic DNA from Blood from subject BoA381

source_name: Blood from subject BoA381


title: Genomic DNA from Blood from subject BoA379

source_name: Blood from subject BoA379


title: Genomic DNA from Blood from subject BoA378

source_name: Blood from subject BoA378


title: Genomic DNA from Blood from subject BoA377

source_name: Blood from subject BoA377


title: Genomic DNA from Blood from subject BoA376

source_name: Blood from subject BoA376


title: Genomic DNA from Blood from subject BoA373

source_name: Blood from subject BoA373


title: Genomic DNA from Blood from subject BoA374

source_name: Blood from subject BoA374


title: Genomic DNA from Blood from subject BoA375

source_name: Blood from subject BoA375


title: Genomic DNA from Blood from subject BoA372

source_name: Blood from subject BoA372


title: Genomic DNA from Blood from subject BoA369

source_name: Blood from subject BoA369


title: Genomic DNA from Blood from subject BoA370

source_name: Blood from subject BoA370


title: Genomic DNA from Blood from subject BoA368

source_name: Blood from subject BoA368


title: Genomic DNA from Blood from subject BoA367

source_name: Blood from subject BoA367


title: Genomic DNA from Blood from subject BoA365

source_name: Blood from subject BoA365


title: Genomic DNA from Blood from subject BoA364

source_name: Blood from subject BoA364


title: Genomic DNA from Blood from subject BoA362

source_name: Blood from subject BoA362


title: Genomic DNA from Blood from subject BoA359

source_name: Blood from subject BoA359


title: Genomic DNA from Blood from subject BoA360

source_name: Blood from subject BoA360


title: Genomic DNA from Blood from subject BoA366

source_name: Blood from subject BoA366


title: Genomic DNA from Blood from subject BoA363

source_name: Blood from subject BoA363


title: Genomic DNA from Blood from subject BoA358

source_name: Blood from subject BoA358


title: Genomic DNA from Blood from subject BoA361

source_name: Blood from subject BoA361


title: Genomic DNA from Blood from subject BoA356

source_name: Blood from subject BoA356


title: Genomic DNA from Blood from subject BoA357

source_name: Blood from subject BoA357


title: Genomic DNA from Blood from subject BoA355

source_name: Blood from subject BoA355


title: Genomic DNA from Blood from subject BoA354

source_name: Blood from subject BoA354


title: Genomic DNA from Blood from subject BoA353

source_name: Blood from subject BoA353


title: Genomic DNA from Blood from subject BoA349

source_name: Blood from subject BoA349


title: Genomic DNA from Blood from subject BoA384

source_name: Blood from subject BoA384


title: Genomic DNA from Blood from subject BoA347

source_name: Blood from subject BoA347


title: Genomic DNA from Blood from subject BoA352

source_name: Blood from subject BoA352


title: Genomic DNA from Blood from subject BoA345

source_name: Blood from subject BoA345


title: Genomic DNA from Blood from subject BoA351

source_name: Blood from subject BoA351


title: Genomic DNA from Blood from subject BoA350

source_name: Blood from subject BoA350


title: Genomic DNA from Blood from subject BoA348

source_name: Blood from subject BoA348


title: Genomic DNA from Blood from subject BoA346

source_name: Blood from subject BoA346


title: Genomic DNA from Blood from subject BoA343

source_name: Blood from subject BoA343


title: Genomic DNA from Blood from subject BoA344

source_name: Blood from subject BoA344


title: Genomic DNA from Blood from subject BoA371

source_name: Blood from subject BoA371


title: Genomic DNA from Blood from subject BoA342

source_name: Blood from subject BoA342

value: Unavailable


title: Genomic DNA from Blood from subject BoA341

source_name: Blood from subject BoA341


title: Genomic DNA from Blood from subject BoA340

source_name: Blood from subject BoA340


title: Genomic DNA from Blood from subject BoA339

source_name: Blood from subject BoA339


title: Genomic DNA from Blood from subject BoA338

source_name: Blood from subject BoA338


title: Genomic DNA from Blood from subject BoA337

source_name: Blood from subject BoA337


title: Genomic DNA from Blood from subject BoA336

source_name: Blood from subject BoA336


title: Genomic DNA from Blood from subject BoA334

source_name: Blood from subject BoA334


title: Genomic DNA from Blood from subject BoA333

source_name: Blood from subject BoA333


title: Genomic DNA from Blood from subject BoA331

source_name: Blood from subject BoA331


title: Genomic DNA from Blood from subject BoA332

source_name: Blood from subject BoA332


title: Genomic DNA from Blood from subject BoA330

source_name: Blood from subject BoA330


title: Genomic DNA from Blood from subject BoA329

source_name: Blood from subject BoA329


title: Genomic DNA from Blood from subject BoA326

source_name: Blood from subject BoA326


title: Genomic DNA from Blood from subject BoA327

source_name: Blood from subject BoA327


title: Genomic DNA from Blood from subject BoA325

source_name: Blood from subject BoA325


title: Genomic DNA from Blood from subject BoA328

source_name: Blood from subject BoA328


title: Genomic DNA from Blood from subject BoA323

source_name: Blood from subject BoA323


title: Genomic DNA from Blood from subject BoA315

source_name: Blood from subject BoA315


title: Genomic DNA from Blood from subject BoA318

source_name: Blood from subject BoA318


title: Genomic DNA from Blood from subject BoA317

source_name: Blood from subject BoA317


title: Genomic DNA from Blood from subject BoA320

source_name: Blood from subject BoA320


title: Genomic DNA from Blood from subject BoA321

source_name: Blood from subject BoA321


title: Genomic DNA from Blood from subject BoA322

source_name: Blood from subject BoA322


title: Genomic DNA from Blood from subject BoA324

source_name: Blood from subject BoA324


title: Genomic DNA from Blood from subject BoA319

source_name: Blood from subject BoA319


title: Genomic DNA from Blood from subject BoA313

source_name: Blood from subject BoA313

value: Declined


title: Genomic DNA from Blood from subject BoA314

source_name: Blood from subject BoA314


title: Genomic DNA from Blood from subject BoA316

source_name: Blood from subject BoA316


title: Genomic DNA from Blood from subject BoA310

source_name: Blood from subject BoA310


title: Genomic DNA from Blood from subject BoA312

source_name: Blood from subject BoA312


title: Genomic DNA from Blood from subject BoA335

source_name: Blood from subject BoA335


title: Genomic DNA from Blood from subject BoA309

source_name: Blood from subject BoA309


title: Genomic DNA from Blood from subject BoA307

source_name: Blood from subject BoA307


title: Genomic DNA from Blood from subject BoA305

source_name: Blood from subject BoA305


title: Genomic DNA from Blood from subject BoA306

source_name: Blood from subject BoA306


title: Genomic DNA from Blood from subject BoA303

source_name: Blood from subject BoA303


title: Genomic DNA from Blood from subject BoA304

source_name: Blood from subject BoA304


title: Genomic DNA from Blood from subject BoA302

source_name: Blood from subject BoA302


title: Genomic DNA from Blood from subject BoA301

source_name: Blood from subject BoA301


title: Genomic DNA from Blood from subject BoA308

source_name: Blood from subject BoA308


title: Genomic DNA from Blood from subject BoA298

source_name: Blood from subject BoA298


title: Genomic DNA from Blood from subject BoA300

source_name: Blood from subject BoA300


title: Genomic DNA from Blood from subject BoA299

source_name: Blood from subject BoA299


title: Genomic DNA from Blood from subject BoA296

source_name: Blood from subject BoA296


title: Genomic DNA from Blood from subject BoA295

source_name: Blood from subject BoA295


title: Genomic DNA from Blood from subject BoA290

source_name: Blood from subject BoA290


title: Genomic DNA from Blood from subject BoA293

source_name: Blood from subject BoA293


title: Genomic DNA from Blood from subject BoA311

source_name: Blood from subject BoA311


title: Genomic DNA from Blood from subject BoA294

source_name: Blood from subject BoA294


title: Genomic DNA from Blood from subject BoA291

source_name: Blood from subject BoA291


title: Genomic DNA from Blood from subject BoA287

source_name: Blood from subject BoA287


title: Genomic DNA from Blood from subject BoA292

source_name: Blood from subject BoA292


title: Genomic DNA from Blood from subject BoA284

source_name: Blood from subject BoA284


title: Genomic DNA from Blood from subject BoA289

source_name: Blood from subject BoA289


title: Genomic DNA from Blood from subject BoA286

source_name: Blood from subject BoA286


title: Genomic DNA from Blood from subject BoA282

source_name: Blood from subject BoA282


title: Genomic DNA from Blood from subject BoA281

source_name: Blood from subject BoA281


title: Genomic DNA from Blood from subject BoA283

source_name: Blood from subject BoA283


title: Genomic DNA from Blood from subject BoA279

source_name: Blood from subject BoA279


title: Genomic DNA from Blood from subject BoA276

source_name: Blood from subject BoA276


title: Genomic DNA from Blood from subject BoA275

source_name: Blood from subject BoA275


title: Genomic DNA from Blood from subject BoA277

source_name: Blood from subject BoA277


title: Genomic DNA from Blood from subject BoA297

source_name: Blood from subject BoA297


title: Genomic DNA from Blood from subject BoA274

source_name: Blood from subject BoA274


title: Genomic DNA from Blood from subject BoA272

source_name: Blood from subject BoA272


title: Genomic DNA from Blood from subject BoA271

source_name: Blood from subject BoA271


title: Genomic DNA from Blood from subject BoA269

source_name: Blood from subject BoA269


title: Genomic DNA from Blood from subject BoA267

source_name: Blood from subject BoA267


title: Genomic DNA from Blood from subject BoA288

source_name: Blood from subject BoA288


title: Genomic DNA from Blood from subject BoA265

source_name: Blood from subject BoA265


title: Genomic DNA from Blood from subject BoA263

source_name: Blood from subject BoA263


title: Genomic DNA from Blood from subject BoA264

source_name: Blood from subject BoA264


title: Genomic DNA from Blood from subject BoA285

source_name: Blood from subject BoA285


title: Genomic DNA from Blood from subject BoA262

source_name: Blood from subject BoA262


title: Genomic DNA from Blood from subject BoA260

source_name: Blood from subject BoA260


title: Genomic DNA from Blood from subject BoA261

source_name: Blood from subject BoA261


title: Genomic DNA from Blood from subject BoA257

source_name: Blood from subject BoA257


title: Genomic DNA from Blood from subject BoA258

source_name: Blood from subject BoA258


title: Genomic DNA from Blood from subject BoA256

source_name: Blood from subject BoA256


title: Genomic DNA from Blood from subject BoA280

source_name: Blood from subject BoA280


title: Genomic DNA from Blood from subject BoA254

source_name: Blood from subject BoA254


title: Genomic DNA from Blood from subject BoA255

source_name: Blood from subject BoA255


title: Genomic DNA from Blood from subject BoA278

source_name: Blood from subject BoA278


title: Genomic DNA from Blood from subject BoA253

source_name: Blood from subject BoA253


title: Genomic DNA from Blood from subject BoA252

source_name: Blood from subject BoA252


title: Genomic DNA from Blood from subject BoA250

source_name: Blood from subject BoA250


title: Genomic DNA from Blood from subject BoA251

source_name: Blood from subject BoA251


title: Genomic DNA from Blood from subject BoA270

source_name: Blood from subject BoA270


title: Genomic DNA from Blood from subject BoA273

source_name: Blood from subject BoA273


title: Genomic DNA from Blood from subject BoA249

source_name: Blood from subject BoA249


title: Genomic DNA from Blood from subject BoA248

source_name: Blood from subject BoA248


title: Genomic DNA from Blood from subject BoA268

source_name: Blood from subject BoA268


title: Genomic DNA from Blood from subject BoA247

source_name: Blood from subject BoA247


title: Genomic DNA from Blood from subject BoA266

source_name: Blood from subject BoA266


title: Genomic DNA from Blood from subject BoA246

source_name: Blood from subject BoA246


title: Genomic DNA from Blood from subject BoA245

source_name: Blood from subject BoA245


title: Genomic DNA from Blood from subject BoA259

source_name: Blood from subject BoA259


title: Genomic DNA from Blood from subject BoA244

source_name: Blood from subject BoA244


title: Genomic DNA from Blood from subject BoA241

source_name: Blood from subject BoA241


title: Genomic DNA from Blood from subject BoA242

source_name: Blood from subject BoA242


title: Genomic DNA from Blood from subject BoA240

source_name: Blood from subject BoA240


title: Genomic DNA from Blood from subject BoA243

source_name: Blood from subject BoA243


title: Genomic DNA from Blood from subject BoA236

source_name: Blood from subject BoA236


title: Genomic DNA from Blood from subject BoA234

source_name: Blood from subject BoA234


title: Genomic DNA from Blood from subject BoA238

source_name: Blood from subject BoA238


title: Genomic DNA from Blood from subject BoA235

source_name: Blood from subject BoA235


title: Genomic DNA from Blood from subject BoA233

source_name: Blood from subject BoA233


title: Genomic DNA from Blood from subject BoA232

source_name: Blood from subject BoA232


title: Genomic DNA from Blood from subject BoA239

source_name: Blood from subject BoA239


title: Genomic DNA from Blood from subject BoA237

source_name: Blood from subject BoA237


title: Genomic DNA from Blood from subject BoA231

source_name: Blood from subject BoA231


title: Genomic DNA from Blood from subject BoA230

source_name: Blood from subject BoA230


title: Genomic DNA from Blood from subject BoA229

source_name: Blood from subject BoA229


title: Genomic DNA from Blood from subject BoA228

source_name: Blood from subject BoA228


title: Genomic DNA from Blood from subject BoA227

source_name: Blood from subject BoA227


title: Genomic DNA from Blood from subject BoA224

source_name: Blood from subject BoA224


title: Genomic DNA from Blood from subject BoA223

source_name: Blood from subject BoA223


title: Genomic DNA from Blood from subject BoA226

source_name: Blood from subject BoA226


title: Genomic DNA from Blood from subject BoA225

source_name: Blood from subject BoA225


title: Genomic DNA from Blood from subject BoA221

source_name: Blood from subject BoA221


title: Genomic DNA from Blood from subject BoA220

source_name: Blood from subject BoA220


title: Genomic DNA from Blood from subject BoA222

source_name: Blood from subject BoA222


title: Genomic DNA from Blood from subject BoA218

source_name: Blood from subject BoA218


title: Genomic DNA from Blood from subject BoA217

source_name: Blood from subject BoA217


title: Genomic DNA from Blood from subject BoA216

source_name: Blood from subject BoA216


title: Genomic DNA from Blood from subject BoA215

source_name: Blood from subject BoA215


title: Genomic DNA from Blood from subject BoA210

source_name: Blood from subject BoA210


title: Genomic DNA from Blood from subject BoA214

source_name: Blood from subject BoA214


title: Genomic DNA from Blood from subject BoA213

source_name: Blood from subject BoA213


title: Genomic DNA from Blood from subject BoA211

source_name: Blood from subject BoA211


title: Genomic DNA from Blood from subject BoA209

source_name: Blood from subject BoA209


title: Genomic DNA from Blood from subject BoA212

source_name: Blood from subject BoA212


title: Genomic DNA from Blood from subject BoA206

source_name: Blood from subject BoA206


title: Genomic DNA from Blood from subject BoA203

source_name: Blood from subject BoA203


title: Genomic DNA from Blood from subject BoA205

source_name: Blood from subject BoA205


title: Genomic DNA from Blood from subject BoA208

source_name: Blood from subject BoA208


title: Genomic DNA from Blood from subject BoA204

source_name: Blood from subject BoA204


title: Genomic DNA from Blood from subject BoA200

source_name: Blood from subject BoA200


title: Genomic DNA from Blood from subject BoA201

source_name: Blood from subject BoA201


title: Genomic DNA from Blood from subject BoA207

source_name: Blood from subject BoA207


title: Genomic DNA from Blood from subject BoA198

source_name: Blood from subject BoA198


title: Genomic DNA from Blood from subject BoA199

source_name: Blood from subject BoA199


title: Genomic DNA from Blood from subject BoA197

source_name: Blood from subject BoA197


title: Genomic DNA from Blood from subject BoA196

source_name: Blood from subject BoA196


title: Genomic DNA from Blood from subject BoA219

source_name: Blood from subject BoA219


title: Genomic DNA from Blood from subject BoA195

source_name: Blood from subject BoA195


title: Genomic DNA from Blood from subject BoA193

source_name: Blood from subject BoA193


title: Genomic DNA from Blood from subject BoA194

source_name: Blood from subject BoA194


title: Genomic DNA from Blood from subject BoA191

source_name: Blood from subject BoA191


title: Genomic DNA from Blood from subject BoA190

source_name: Blood from subject BoA190


title: Genomic DNA from Blood from subject BoA189

source_name: Blood from subject BoA189


title: Genomic DNA from Blood from subject BoA187

source_name: Blood from subject BoA187


title: Genomic DNA from Blood from subject BoA185

source_name: Blood from subject BoA185


title: Genomic DNA from Blood from subject BoA186

source_name: Blood from subject BoA186


title: Genomic DNA from Blood from subject BoA181

source_name: Blood from subject BoA181


title: Genomic DNA from Blood from subject BoA182

source_name: Blood from subject BoA182


title: Genomic DNA from Blood from subject BoA184

source_name: Blood from subject BoA184


title: Genomic DNA from Blood from subject BoA202

source_name: Blood from subject BoA202


title: Genomic DNA from Blood from subject BoA183

source_name: Blood from subject BoA183


title: Genomic DNA from Blood from subject BoA177

source_name: Blood from subject BoA177


title: Genomic DNA from Blood from subject BoA178

source_name: Blood from subject BoA178


title: Genomic DNA from Blood from subject BoA179

source_name: Blood from subject BoA179


title: Genomic DNA from Blood from subject BoA180

source_name: Blood from subject BoA180


title: Genomic DNA from Blood from subject BoA175

source_name: Blood from subject BoA175


title: Genomic DNA from Blood from subject BoA176

source_name: Blood from subject BoA176


title: Genomic DNA from Blood from subject BoA174

source_name: Blood from subject BoA174


title: Genomic DNA from Blood from subject BoA172

source_name: Blood from subject BoA172


title: Genomic DNA from Blood from subject BoA173

source_name: Blood from subject BoA173


title: Genomic DNA from Blood from subject BoA169

source_name: Blood from subject BoA169


title: Genomic DNA from Blood from subject BoA170

source_name: Blood from subject BoA170


title: Genomic DNA from Blood from subject BoA188

source_name: Blood from subject BoA188


title: Genomic DNA from Blood from subject BoA171

source_name: Blood from subject BoA171


title: Genomic DNA from Blood from subject BoA168

source_name: Blood from subject BoA168


title: Genomic DNA from Blood from subject BoA166

source_name: Blood from subject BoA166


title: Genomic DNA from Blood from subject BoA167

source_name: Blood from subject BoA167


title: Genomic DNA from Blood from subject BoA164

source_name: Blood from subject BoA164


title: Genomic DNA from Blood from subject BoA192

source_name: Blood from subject BoA192


title: Genomic DNA from Blood from subject BoA161

source_name: Blood from subject BoA161


title: Genomic DNA from Blood from subject BoA163

source_name: Blood from subject BoA163


title: Genomic DNA from Blood from subject BoA165

source_name: Blood from subject BoA165


title: Genomic DNA from Blood from subject BoA162

source_name: Blood from subject BoA162


title: Genomic DNA from Blood from subject BoA160

source_name: Blood from subject BoA160


title: Genomic DNA from Blood from subject BoA154

source_name: Blood from subject BoA154


title: Genomic DNA from Blood from subject BoA158

source_name: Blood from subject BoA158


title: Genomic DNA from Blood from subject BoA155

source_name: Blood from subject BoA155


title: Genomic DNA from Blood from subject BoA156

source_name: Blood from subject BoA156


title: Genomic DNA from Blood from subject BoA157

source_name: Blood from subject BoA157


title: Genomic DNA from Blood from subject BoA153

source_name: Blood from subject BoA153


title: Genomic DNA from Blood from subject BoA151

source_name: Blood from subject BoA151


title: Genomic DNA from Blood from subject BoA159

source_name: Blood from subject BoA159


title: Genomic DNA from Blood from subject BoA150

source_name: Blood from subject BoA150


title: Genomic DNA from Blood from subject BoA152

source_name: Blood from subject BoA152


title: Genomic DNA from Blood from subject BoA146

source_name: Blood from subject BoA146


title: Genomic DNA from Blood from subject BoA143

source_name: Blood from subject BoA143


title: Genomic DNA from Blood from subject BoA148

source_name: Blood from subject BoA148


title: Genomic DNA from Blood from subject BoA140

source_name: Blood from subject BoA140


title: Genomic DNA from Blood from subject BoA138

source_name: Blood from subject BoA138


title: Genomic DNA from Blood from subject BoA139

source_name: Blood from subject BoA139


title: Genomic DNA from Blood from subject BoA141

source_name: Blood from subject BoA141


title: Genomic DNA from Blood from subject BoA142

source_name: Blood from subject BoA142


title: Genomic DNA from Blood from subject BoA137

source_name: Blood from subject BoA137


title: Genomic DNA from Blood from subject BoA144

source_name: Blood from subject BoA144

value: American Indian or Alaska Native


title: Genomic DNA from Blood from subject BoA133

source_name: Blood from subject BoA133


title: Genomic DNA from Blood from subject BoA132

source_name: Blood from subject BoA132


title: Genomic DNA from Blood from subject BoA131

source_name: Blood from subject BoA131


title: Genomic DNA from Blood from subject BoA134

source_name: Blood from subject BoA134


title: Genomic DNA from Blood from subject BoA135

source_name: Blood from subject BoA135


title: Genomic DNA from Blood from subject BoA149

source_name: Blood from subject BoA149


title: Genomic DNA from Blood from subject BoA147

source_name: Blood from subject BoA147


title: Genomic DNA from Blood from subject BoA128

source_name: Blood from subject BoA128


title: Genomic DNA from Blood from subject BoA127

source_name: Blood from subject BoA127


title: Genomic DNA from Blood from subject BoA145

source_name: Blood from subject BoA145


title: Genomic DNA from Blood from subject BoA124

source_name: Blood from subject BoA124


title: Genomic DNA from Blood from subject BoA126

source_name: Blood from subject BoA126


title: Genomic DNA from Blood from subject BoA129

source_name: Blood from subject BoA129


title: Genomic DNA from Blood from subject BoA123

source_name: Blood from subject BoA123


title: Genomic DNA from Blood from subject BoA122

source_name: Blood from subject BoA122


title: Genomic DNA from Blood from subject BoA121

source_name: Blood from subject BoA121


title: Genomic DNA from Blood from subject BoA136

source_name: Blood from subject BoA136


title: Genomic DNA from Blood from subject BoA118

source_name: Blood from subject BoA118


title: Genomic DNA from Blood from subject BoA119

source_name: Blood from subject BoA119


title: Genomic DNA from Blood from subject BoA120

source_name: Blood from subject BoA120


title: Genomic DNA from Blood from subject BoA117

source_name: Blood from subject BoA117


title: Genomic DNA from Blood from subject BoA113

source_name: Blood from subject BoA113


title: Genomic DNA from Blood from subject BoA114

source_name: Blood from subject BoA114


title: Genomic DNA from Blood from subject BoA111

source_name: Blood from subject BoA111


title: Genomic DNA from Blood from subject BoA112

source_name: Blood from subject BoA112


title: Genomic DNA from Blood from subject BoA110

source_name: Blood from subject BoA110


title: Genomic DNA from Blood from subject BoA116

source_name: Blood from subject BoA116


title: Genomic DNA from Blood from subject BoA115

source_name: Blood from subject BoA115


title: Genomic DNA from Blood from subject BoA109

source_name: Blood from subject BoA109


title: Genomic DNA from Blood from subject BoA108

source_name: Blood from subject BoA108


title: Genomic DNA from Blood from subject BoA107

source_name: Blood from subject BoA107


title: Genomic DNA from Blood from subject BoA105

source_name: Blood from subject BoA105


title: Genomic DNA from Blood from subject BoA106

source_name: Blood from subject BoA106


title: Genomic DNA from Blood from subject BoA130

source_name: Blood from subject BoA130


title: Genomic DNA from Blood from subject BoA125

source_name: Blood from subject BoA125


title: Genomic DNA from Blood from subject BoA104

source_name: Blood from subject BoA104


title: Genomic DNA from Blood from subject BoA103

source_name: Blood from subject BoA103


title: Genomic DNA from Blood from subject BoA101

source_name: Blood from subject BoA101


title: Genomic DNA from Blood from subject BoA100

source_name: Blood from subject BoA100


title: Genomic DNA from Blood from subject BoA102

source_name: Blood from subject BoA102


title: Genomic DNA from Blood from subject BoA97

source_name: Blood from subject BoA97


title: Genomic DNA from Blood from subject BoA98

source_name: Blood from subject BoA98


title: Genomic DNA from Blood from subject BoA96

source_name: Blood from subject BoA96


title: Genomic DNA from Blood from subject BoA94

source_name: Blood from subject BoA94


title: Genomic DNA from Blood from subject BoA93

source_name: Blood from subject BoA93


title: Genomic DNA from Blood from subject BoA89

source_name: Blood from subject BoA89


title: Genomic DNA from Blood from subject BoA95

source_name: Blood from subject BoA95


title: Genomic DNA from Blood from subject BoA88

source_name: Blood from subject BoA88


title: Genomic DNA from Blood from subject BoA85

source_name: Blood from subject BoA85


title: Genomic DNA from Blood from subject BoA86

source_name: Blood from subject BoA86


title: Genomic DNA from Blood from subject BoA91

source_name: Blood from subject BoA91


title: Genomic DNA from Blood from subject BoA87

source_name: Blood from subject BoA87


title: Genomic DNA from Blood from subject BoA81

source_name: Blood from subject BoA81


title: Genomic DNA from Blood from subject BoA82

source_name: Blood from subject BoA82


title: Genomic DNA from Blood from subject BoA78

source_name: Blood from subject BoA78


title: Genomic DNA from Blood from subject BoA79

source_name: Blood from subject BoA79


title: Genomic DNA from Blood from subject BoA75

source_name: Blood from subject BoA75


title: Genomic DNA from Blood from subject BoA76

source_name: Blood from subject BoA76


title: Genomic DNA from Blood from subject BoA77

source_name: Blood from subject BoA77


title: Genomic DNA from Blood from subject BoA99

source_name: Blood from subject BoA99


title: Genomic DNA from Blood from subject BoA70

source_name: Blood from subject BoA70


title: Genomic DNA from Blood from subject BoA73

source_name: Blood from subject BoA73


title: Genomic DNA from Blood from subject BoA90

source_name: Blood from subject BoA90


title: Genomic DNA from Blood from subject BoA74

source_name: Blood from subject BoA74


title: Genomic DNA from Blood from subject BoA71

source_name: Blood from subject BoA71


title: Genomic DNA from Blood from subject BoA66

source_name: Blood from subject BoA66


title: Genomic DNA from Blood from subject BoA72

source_name: Blood from subject BoA72


title: Genomic DNA from Blood from subject BoA69

source_name: Blood from subject BoA69


title: Genomic DNA from Blood from subject BoA65

source_name: Blood from subject BoA65


title: Genomic DNA from Blood from subject BoA92

source_name: Blood from subject BoA92


title: Genomic DNA from Blood from subject BoA64

source_name: Blood from subject BoA64


title: Genomic DNA from Blood from subject BoA68

source_name: Blood from subject BoA68


title: Genomic DNA from Blood from subject BoA63

source_name: Blood from subject BoA63


title: Genomic DNA from Blood from subject BoA83

source_name: Blood from subject BoA83


title: Genomic DNA from Blood from subject BoA67

source_name: Blood from subject BoA67


title: Genomic DNA from Blood from subject BoA84

source_name: Blood from subject BoA84


title: Genomic DNA from Blood from subject BoA80

source_name: Blood from subject BoA80


title: Genomic DNA from Blood from subject BoA62

source_name: Blood from subject BoA62


title: Genomic DNA from Blood from subject BoA58

source_name: Blood from subject BoA58


title: Genomic DNA from Blood from subject BoA56

source_name: Blood from subject BoA56


title: Genomic DNA from Blood from subject BoA59

source_name: Blood from subject BoA59


title: Genomic DNA from Blood from subject BoA61

source_name: Blood from subject BoA61


title: Genomic DNA from Blood from subject BoA60

source_name: Blood from subject BoA60


title: N2A cells, OICR.KI.2016_1_P14

status: Public on May 28 2024

first: Ulrich

last: Braunschweig

address: 160 College Street

department: Donnelly Centre

institute: University of Toronto

data_processing:
Base-calling Q-scores were obtained with RTA 3.4.4
For alternative splicing analysis, RNA-Seq data were processed using the vast-tools pipeline, version 2.1 https://github.com/vastgroup/vast-tools (Braunschweig et al., 2014; Irimia et al., 2014; Tapial et al., 2017). Events were filtered for coverage and junction balance, requiring a vast-tools quality column score 3 of SOK/OK/LOW for cassette exon (CE), microexon (MIC), and alternative 5' or 3' splice site (Alt5/3) events or at least 15 reads for intron retention (IR) events and a balance score (score 4) of ‘OK’ or ‘B1’ for alternative exons or > 0.05 for intron retention events, in at least half of replicate experiments. If more than one replicates was present, the point estimate from vast-tools diff was used and if the coverage/balance filtering step failed for an event in more than half of the replicates, its value was set to NA. Changes were considered significant if they were greater than 10 ∆PSI/dPIR and the expected minimum change was different from zero at p > 0.95 according to vast-tools’ diff module.
Gene expression changes were analyzed by pseudo-aligning pre-trimmed reads to GENCODE vM17 transcripts using Salmon v0.10.2 (Patro et al., 2017) and aggregated per gene using the R package tximport (Soneson et al., 2015). Differential expression was then assessed using the classic mode (exactTest) edgeR (McCarthy et al., 2012), and genes with an FDR < 0.05 and a greater than 2-fold change were considered differential. For each comparison, only genes expressed at a minimum of 5 RPKM in one or both conditions were considered.
Assembly: mm9
Supplementary files format and content: vast-tools.AltSplicing.tab.gz is a tab-delimited file and contains vast-tools output, i.e. event information and raw percent-spliced-in values and quality scores per sample. See https://github.com/vastgroup/vast-tools#combine-output-format.
Supplementary files format and content: AltSplicing.tab.gz is a tab-delimited file and contains mean percent-splice-in values per treatment and significance calls for the difference vs. DMSO control.
Supplementary files format and content: Expression.tab.gz is a tab-delimited file and contains RPKM, log2 fold-change and p-values per treatment vs, DMSO control.

growth_protocol: Mouse Neuro-2A (N2A) cells were grown in DMEM supplemented with 10% FBS, sodium pyruvate, MEM non-essential amino acids, and penicillin/streptomycin, and maintained at 37°C with 5% CO2.

extract_protocol:
Total RNA purification was performed using RNeasy spin columns (Qiagen) following manufacturer's instructions. An on-column DnaseI treatment was performed using RNase-free DNase set (Qiagen) following manufacturer's instructions.
Illumina TruSeq RNA Library Prep Kit v2

treatment_protocol: N2A cells were seeded into 6-well plates. The following day, cells were treated with small molecules for a period of 24 hours (0.4% DMSO final concentration).

value: Mouse neuroblastoma cells


title: N2A cells, OICR.24K.Dissimilar_18_P14


title: N2A cells, OICR.KI.2016_2_B15


title: Genomic DNA from Blood from subject BoA57

source_name: Blood from subject BoA57


title: N2A cells, Maybridge_26_F05


title: N2A cells, Maybridge_67_G04


title: N2A cells, OICR.24K.Dissimilar_52_B08


title: N2A cells, Maybridge_121_K16


title: N2A cells, Maybridge_143_I12


title: N2A cells, Maybridge_98_D13


title: N2A cells, OICR.24K.Dissimilar_55_A11


title: N2A cells, OICR.24K.Dissimilar_47_J19


title: N2A cells, Maybridge_138_F05


title: N2A cells, Lopac_1_G11


title: N2A cells, Maybridge_22_G18


title: N2A cells, OICR.24K.Dissimilar_40_D08


title: N2A cells, Vorinostat_SAHA, replicate B


title: N2A cells, Maybridge_109_D22


title: N2A cells, OICR.KI.2016_2_G19


title: N2A cells, OICR.KI.2016_2_O14


title: N2A cells, Maybridge_21_O19


title: N2A cells, Maybridge_2_M15


title: N2A cells, OICR.KI.2016_2_L17


title: N2A cells, CI_994, replicate B


title: N2A cells, CI_994, replicate A


title: N2A cells, BRD6688, replicate A


title: N2A cells, BRD4884, replicate B


title: N2A cells, OICR.KI.2016_1_D05


title: N2A cells, BRD4884, replicate A


title: N2A cells, BRD6688, replicate B


title: N2A cells, DMSO, replicate C

value: DMSO


title: N2A cells, DMSO, replicate B


title: N2A cells, OICR.KI.2016_1_M04


title: STAD-G3

status: Public on May 23 2024

first: Linghua

last: Wang

address: 1881 East Road,

institute: MD Anderson Cancer Center

data_processing:
METI takes spatial gene expression and histology image data as input. The ST gene expression data contains an N × M matrix of unique molecular identifier (UMI) counts, where N denotes the number of spots and M represents the number of genes. Each spot is associated with 2-dimensional spatial coordinates denoted as (x, y). The gene expression values for each spot are normalized by dividing the UMI count of each gene within that spot by the overall UMI count of all genes in the same spot. The result is then scaled up by a factor of 10,000 and converted to a natural logarithm scale.
Supplementary files format and content: Matrix
Library strategy: Spatial Transcriptomics

source_name: gastric adenocarcinoma

extract_protocol:
ST on FFPE slides were performed with the Visium spatial technology from 10X Genomics. Two to three consecutive tissue sections of 5-μm thickness were collected for RNA extraction with the Qiagen RNeasy FFPE Kit. To assess the RNA quality of the tissue, the purified RNA was immediately processed to calculate the percentage of total RNA fragments >200 nucleotides (DV200) using the Agilent RNA 6000 Pico Kit. Based on DV200 evaluation, blocks with DV200 >30% were selected for proceeding with sectioning. The area of interest (11 x 11 mm) on section was carefully placed within the allowable area to ensure compatibility with the Visium CytAssist instrument. The tissues were then deparaffinized, stained, and decross-linked, followed by probe hybridization, ligation, CytAssist enabled RNA digestion and oligo capture, release, and extension.
The Visium spatial gene expression FFPE libraries were constructed using the Visium CytAssist Spatial Gene Expression for FFPE Human Transcriptome Probe Kit (PN-1000444) following the manufacturer's guidance. Constructed libraries were sequenced on the Illumina NovaSeq 6000 platforms to achieve a depth of at least 75,000 mean read pairs per spot.

value: gastric adenocarcinoma


title: N2A cells, DMSO, replicate A


title: STAD-G2


title: STAD-G4


title: BLCA-B1

source_name: bladder cancer

value: bladder cancer


title: N2A cells, Vorinostat_SAHA, replicate A


title: BLCA-B2


title: Bmp4 germline KO plate 26

status: Public on May 21 2024

city: Rehovot

first: Markus

last: Mittnenzweig

state: Shfela

address: 234 Herzl St.

country: Israel

institute: Weizmann Institute of Science

description:
germline_x6_p11
MARS-seq of F1 generation embryos of Bmp4 KO Heterozygous mice

data_processing:
MARS-seq data was processed as detailed in the MARS-seq2.0 protocol (Keren-Shaul et al. 2019):
see https://github.com/tanaylab/MARS-SEQ for computational pipeline
1. bcl2fastq/2.15.0.4
2. Pool_barcode (4 bps) and well_barcode (7 bps) plus UMI barcode (8 bps) were removed from Read 1 and Read 2 (respectively)
3. Read 1 sequences were mapped to the mm10 genome using star aligner
4. Demultiplexing and construction of mRNA count matrix were based on 4bp plate barcode, 7bp well barcode and 8bp UMI barcode
read1 structure: 52bp mRNA, 4bp plate barcode was added to the fastq header
read2 structure: 7W.8R, i.e. first 7bp contain the cell barcode and next 8bp are UMI
Assembly: mm10
Supplementary files format and content: Tab-separated text file for each 384-well plate containing UMI counts per gene per single cell. Supplementary files contain metadata for each processed single cell
10x scRNA-seq data from ex utero embryos was processed using Cell Ranger v6.2.1
After initial demultiplexing, reads were aligned and counted using cellranger count with default settings
Supplementary files format and content: filtered feature barcode matrix in .mtx format (10x output of cellranger count pipeline)

source_name: Mouse embryo

growth_protocol: control and mutant mESCs were cultured on irradiated mouse embryonic fibroblasts (MEFs) with standard serum medium for 2-3 days and replaced with 2i+LIF medium containing an additional 1 mM PD0325901 and 3 mM CHIR99021 for another growth of 10-12 hours.

extract_protocol:
Genetically manipulated embryos were dissected in ice-cold 1×PBS, followed by imaging in a drop of DMEM medium supplemented with 10% foetal bovine serum (FBS) under a Nikon Eclipse Ti2 inverted microscope. Embryos were then rinsed in ice-cold 1×PBS, transferred into an Eppendorf tube containing 35 µl 0.25% Trypsin/0.02% EDTA Solution A, and incubated at 37 °C for 5 min with 2-3 times pipetting. Trypsinization was stopped by adding 10% FBS/DMEM medium. Cell suspensions were filtered through a 35 µm strainer before sorting.
Single-cell cDNA libraries were prepared using the MARS-Seq method as described (Keren-Shaul et al., 2019). Briefly, reverse transcription was performed in individual wells of the sorted 384-well plate to get complementary DNA (cDNA). Subsequently, all liquid from each 384-well plate was pooled and converted to double-stranded DNA, transcribed using T7 RNA polymerase, and fragmented. A unique plate adaptor was added to each library to be sequenced together, and RNA::ssDNA (plate adaptor) ligation was performed. The purified ligates were applied to a second RT step with a primer complementary to the ligated adaptor, and the library is completed by cDNA enrichment through PCR amplification. Library concentration and profile were evaluated before sequencing.
10x ex utero: Cultured embryos were prepared for sequencing at different developmental stages after two days in culture: late streak and early head fold, assessed by light microscopy. Six similar embryos were selected for each developmental stage, pooled and dissociated using Trypsin-EDTA solution A 0.25% (Biological Industries; 030501B) for 5 minutes at 37℃. Trypsin was neutralized with media including 10% FBS and cells were washed and re-suspended in 1x PBS (calcium and magnesium free) with 400 μg/ml BSA. Cell suspension was than filtered with a 100 μm cell strainer to avoid cell clumps. Single cell viability was determined by trypan blue staining, before being diluted to a final concentration of 1000 cells/μl. Single-cell RNA-seq libraries were generated for each pool of embryos separately using 10x Genomics Chromium v3 system (5000 cell target cell recovery) and sequenced on Illumina NovaSeq 6000 platform according to the manufacturer’s instructions.

treatment_protocol: (1) To obtain whole-embryo mutants, mESCs were injected into tetraploid B6D2F x B6D2F1 E3.5 blastocysts and surgically implanted into E2.5 postcoitum pseudo-pregnant Hsd:ICR (CD-1) females following standard procedures. Females were sacrificed at embryonic day 7.5-9.5 based on the design of the experiment. (2) For ex utero experiments - Pre-gastrulating embryos were dissected at E5.5, their Reichert's membrane removed and individually placed into separate wells of 8-well glass bottom/ibiTreat μ-plates (iBidi; 80827/80826) filled with 250 μl of EUCM (consisted of 25% DMEM (GIBCO 11880; includes 1mg/mL D-glucose and pyruvate, without phenol red and without L-glutamine) supplemented with 1x Glutamax (GIBCO, 35050061), 100 units/ml penicillin/100 μg/ml streptomycin (Biological industries; 030311B) and 11 mM HEPES (GIBCO, 15630056), plus 50% Rat Serum (RS) (Rat whole embryo culture serum, ENVIGO Bioproducts B-4520) and 25% Human Umbilical Cord Blood Serum (HCS) that is prepared in-house. Media was pre-heated for an hour in an incubator with 5% CO2 at 37 °C. Embryos were cultured statically with 5% CO2 at 37℃. Total volume of media was replaced every 24 hrs, and embryos were monitored by morphological assessment daily. (3) Elf5 ExE-KO mutants: Infection of nascent blastocysts were performed using B6D2F1 (C57BL/6xDBA) (Envigo)/Cas9-GFP embryos. In brief, 3-4-week old B6D2F1 females were hormone primed by an intraperitoneal (i.p.) injection of pregnant mare serum gonadotropin (PMSG, Vetmarket) followed 46 hr later by an injection of human chorionic gonadotropin (hCG, Sigma) and mating with homozygote Cas9-GFP males. Embryos were harvested at the zygote stage, and cultured in KSOM medium until the blastocyst stage. For efficient infection of the trophectoderm, zona pellucida was removed in acidic Tyrode’s solution (Sigma). Next, 15-20 embryos were incubated with lentiviruses, described above, in KSOM for 4-5 hrs. The transduced blastocysts were washed, and then transferred to each recipient female generated after mating with vasectomized CD1 males (Envigo); the day of injection was considered as 2.5dpc. Mice were handled in accordance with institutional guidelines and approved by the Institutional Animal Care and Use Committee (IACUC).

value: Mouse embryo

value: Bmp4 KO Heterozygous (C57BL/6x129)


title: PGC plate 12

description:
E775_PGC_P01_NOVA
MARS-seq of FACS-sorted Oct4-positive cells

value: transgenic OCT4-EGFP reporter cells


title: Bmp4 germline KO plate 22

description:
germline_x6_p7
MARS-seq of F1 generation embryos of Bmp4 KO Heterozygous mice


title: Bmp4 germline KO plate 24

description:
germline_x6_p9
MARS-seq of F1 generation embryos of Bmp4 KO Heterozygous mice


title: Bmp4 germline KO plate 23

description:
germline_x6_p8
MARS-seq of F1 generation embryos of Bmp4 KO Heterozygous mice


title: STAD-G1


title: Bmp4 germline KO plate 20

description:
germline_x6_p4
MARS-seq of F1 generation embryos of Bmp4 KO Heterozygous mice


title: Bmp4 germline KO plate 17

description:
germline_x6_p1
MARS-seq of F1 generation embryos of Bmp4 KO Heterozygous mice


title: Bmp4 germline KO plate 25

description:
germline_x6_p10
MARS-seq of F1 generation embryos of Bmp4 KO Heterozygous mice


title: Bmp4 germline KO plate 18

description:
germline_x6_p2
MARS-seq of F1 generation embryos of Bmp4 KO Heterozygous mice


title: Late streak stage ex utero embryos

description:
ls_exutero_embryos
10x scRNA-seq of ex utero cultured embryos

value: Ex utero cultured embryo


title: Bmp4 germline KO plate 16

description:
germline_x4_p13
MARS-seq of F1 generation embryos of Bmp4 KO Heterozygous mice


title: Bmp4 germline KO plate 19

description:
germline_x6_p3
MARS-seq of F1 generation embryos of Bmp4 KO Heterozygous mice


title: Bmp4 germline KO plate 15

description:
germline_x4_p12
MARS-seq of F1 generation embryos of Bmp4 KO Heterozygous mice


title: Bmp4 germline KO plate 13

description:
germline_x4_p16
MARS-seq of F1 generation embryos of Bmp4 KO Heterozygous mice


title: Bmp4 germline KO plate 9

description:
germline_x4_p9
MARS-seq of F1 generation embryos of Bmp4 KO Heterozygous mice


title: Bmp4 germline KO plate 10

description:
germline_x4_p10
MARS-seq of F1 generation embryos of Bmp4 KO Heterozygous mice


title: Bmp4 germline KO plate 11

description:
germline_x4_p14
MARS-seq of F1 generation embryos of Bmp4 KO Heterozygous mice


title: Bmp4 germline KO plate 12

description:
germline_x4_p15
MARS-seq of F1 generation embryos of Bmp4 KO Heterozygous mice


title: Bmp4 germline KO plate 8

description:
germline_x4_p8
MARS-seq of F1 generation embryos of Bmp4 KO Heterozygous mice


title: Bmp4 germline KO plate 7

description:
germline_x4_p7
MARS-seq of F1 generation embryos of Bmp4 KO Heterozygous mice


title: Bmp4 germline KO plate 6

description:
germline_x4_p6
MARS-seq of F1 generation embryos of Bmp4 KO Heterozygous mice


title: Bmp4 germline KO plate 2

description:
germline_x4_p2
MARS-seq of F1 generation embryos of Bmp4 KO Heterozygous mice


title: Bmp4 germline KO plate 3

description:
germline_x4_p3
MARS-seq of F1 generation embryos of Bmp4 KO Heterozygous mice


title: Bmp4 germline KO plate 5

description:
germline_x4_p5
MARS-seq of F1 generation embryos of Bmp4 KO Heterozygous mice


title: Head fold stage ex utero embryos

description:
hf_exutero_embryos
10x scRNA-seq of ex utero cultured embryos


title: Bmp4 germline KO plate 1

description:
germline_x4_p1
MARS-seq of F1 generation embryos of Bmp4 KO Heterozygous mice


title: Bmp4 embryonic KO plate 47

description:
4n_x9_p1
MARS-seq of tetraploid complementary assay of embryonic Bmp4-KO embryos

value: Host cells and injected mESC-derived Bmp4 KO cells


title: Bmp4 embryonic KO plate 38

description:
4n_x10_p18
MARS-seq of tetraploid complementary assay of embryonic Bmp4-KO embryos


title: Bmp4 embryonic KO plate 41

description:
4n_x10_p21
MARS-seq of tetraploid complementary assay of embryonic Bmp4-KO embryos


title: Bmp4 embryonic KO plate 44

description:
4n_x10_p24
MARS-seq of tetraploid complementary assay of embryonic Bmp4-KO embryos


title: Bmp4 embryonic KO plate 46

description:
4n_x10_p26
MARS-seq of tetraploid complementary assay of embryonic Bmp4-KO embryos


title: Bmp4 embryonic KO plate 42

description:
4n_x10_p22
MARS-seq of tetraploid complementary assay of embryonic Bmp4-KO embryos


title: Bmp4 embryonic KO plate 36

description:
4n_x10_p16
MARS-seq of tetraploid complementary assay of embryonic Bmp4-KO embryos


title: Bmp4 embryonic KO plate 45

description:
4n_x10_p25
MARS-seq of tetraploid complementary assay of embryonic Bmp4-KO embryos


title: Bmp4 embryonic KO plate 43

description:
4n_x10_p23
MARS-seq of tetraploid complementary assay of embryonic Bmp4-KO embryos


title: Bmp4 embryonic KO plate 39

description:
4n_x10_p19
MARS-seq of tetraploid complementary assay of embryonic Bmp4-KO embryos


title: Bmp4 germline KO plate 14

description:
germline_x4_p11
MARS-seq of F1 generation embryos of Bmp4 KO Heterozygous mice


title: Bmp4 embryonic KO plate 37

description:
4n_x10_p17
MARS-seq of tetraploid complementary assay of embryonic Bmp4-KO embryos


title: Bmp4 embryonic KO plate 40

description:
4n_x10_p20
MARS-seq of tetraploid complementary assay of embryonic Bmp4-KO embryos


title: Bmp4 germline KO plate 21

description:
germline_x6_p5
MARS-seq of F1 generation embryos of Bmp4 KO Heterozygous mice


title: Bmp4 embryonic KO plate 35

description:
4n_x10_p15
MARS-seq of tetraploid complementary assay of embryonic Bmp4-KO embryos


title: Bmp4 embryonic KO plate 34

description:
4n_x10_p14
MARS-seq of tetraploid complementary assay of embryonic Bmp4-KO embryos


title: Bmp4 embryonic KO plate 32

description:
4n_x10_p12
MARS-seq of tetraploid complementary assay of embryonic Bmp4-KO embryos


title: Bmp4 embryonic KO plate 30

description:
4n_x10_p10
MARS-seq of tetraploid complementary assay of embryonic Bmp4-KO embryos


title: Bmp4 germline KO plate 4

description:
germline_x4_p4
MARS-seq of F1 generation embryos of Bmp4 KO Heterozygous mice


title: Bmp4 embryonic KO plate 31

description:
4n_x10_p11
MARS-seq of tetraploid complementary assay of embryonic Bmp4-KO embryos


title: Bmp4 embryonic KO plate 29

description:
4n_x10_p8
MARS-seq of tetraploid complementary assay of embryonic Bmp4-KO embryos


title: Bmp4 embryonic KO plate 28

description:
4n_x10_p7
MARS-seq of tetraploid complementary assay of embryonic Bmp4-KO embryos


title: Bmp4 embryonic KO plate 27

description:
4n_x10_p6
MARS-seq of tetraploid complementary assay of embryonic Bmp4-KO embryos


title: Bmp4 embryonic KO plate 25

description:
4n_x10_p4
MARS-seq of tetraploid complementary assay of embryonic Bmp4-KO embryos


title: Bmp4 embryonic KO plate 24

description:
4n_x10_p3
MARS-seq of tetraploid complementary assay of embryonic Bmp4-KO embryos


title: Bmp4 embryonic KO plate 22

description:
4n_x10_p1
MARS-seq of tetraploid complementary assay of embryonic Bmp4-KO embryos


title: Bmp4 embryonic KO plate 21

description:
4n_x9_p3
MARS-seq of tetraploid complementary assay of embryonic Bmp4-KO embryos


title: Bmp4 embryonic KO plate 19

description:
4n_x8_p6
MARS-seq of tetraploid complementary assay of embryonic Bmp4-KO embryos


title: Bmp4 embryonic KO plate 20

description:
4n_x8_p7
MARS-seq of tetraploid complementary assay of embryonic Bmp4-KO embryos


title: Bmp4 embryonic KO plate 14

description:
4n_x8_p1
MARS-seq of tetraploid complementary assay of embryonic Bmp4-KO embryos


title: Bmp4 embryonic KO plate 16

description:
4n_x8_p3
MARS-seq of tetraploid complementary assay of embryonic Bmp4-KO embryos


title: Bmp4 embryonic KO plate 15

description:
4n_x8_p2
MARS-seq of tetraploid complementary assay of embryonic Bmp4-KO embryos


title: Bmp4 embryonic KO plate 13

description:
4n_x7_p17
MARS-seq of tetraploid complementary assay of embryonic Bmp4-KO embryos


title: Bmp4 embryonic KO plate 17

description:
4n_x8_p4
MARS-seq of tetraploid complementary assay of embryonic Bmp4-KO embryos