title: Lt8133 ChIP H3 2

city: Cold Spring Harbor

last: Martienssen

address: 1 Bungtown Rd

institute: Cold Spring Harbor Laboratory

description: Library name: lt8133-h3-rep2

data_processing:
Initial data analysis including adapter trimming, alignment, and peak calling, was carried out as described in Cahn et al. 2024.
After visualizing CENH3/H3 enrichment, centromere regions were computationally estimated as follows: 1) log2(CENH3/H3) was computed across 10 Kbp genome bins, smoothed by 30 Kbp with deepTools v3.5.5  bamCompare; 2) The bin with the highest enrichment per chromosome was selected, and the intersecting MACS2 131 broad peak call with largest (log2(CENH3/H3) * peak_width) as computed by the MaizeCODE pipeline was chosen as the primary peak with bedtools v2.31.1  intersect and awk; 3) all MACS2 peaks within 5 Kbp of each other were merged, and the merged peaks overlapping the primary peak per chromosome were taken as the predicted centromere regions. Predicted transposable elements overlapping these regions were annotated with DANTE v0.2.5
Assembly: Lj8627-REF-CSHL-1.0, Lm9252-REF-CSHL-1.0, Lt9434-REF-CSHL-1.0
Supplementary files format and content: BigWigs:  independently mapped genome coverage from each replicate, and merged coverage for each condition, generated as described in Cahn et al. 2024.
Supplementary files format and content: BEDs:  predicted centromere regions.

organism: Lemna turionifera

value: whole frond


title: NHP Liver, Vehicle, 2

status: Public on Feb 04 2025

type: SRA

city: Boston

first: Frederic

last: Tremblay

state: MA

address: 201 Brookline Ave, Suite 1101

country: USA

institute: Chroma Medicine

library_source: genomic

data_processing:
A nf-core Methyl-Seq pipeline was used for initial read alignment and estimation of methylation calls using Bismark, aligned to the Ensembl Cyno_6.0 reference genome without deduplication.
IGV was used to visualize the methylation bedgraphs in the region surrounding the PCSK9 TSS.
Average % methylation of CpGs with coverage in all conditions in a 500bp region centered on the gRNA binding sites (Chr1:169045699-169046199) was calculated for each condition using R.
Assembly: Cyno_6.0
Supplementary files format and content: bedgraph, CpG methylation

molecule: genomic DNA

organism: Macaca fascicularis

source_name: Liver

extract_protocol:
Genomic DNA was extracted from NHP liver biopsies using DNAdvance Kit (Beckman Coulter).
Genomic DNA is denatured and treated with sodium bisulfite and heat to deaminate unmethylated cytosines to uracil using the EZ-96 Methylation-Gold MagPrep bisulfite conversion kit (Zymo Research). Primers were designed to amplify the region around the NHP PCSK9 TSS, as seven short, partially overlapping, amplicons. Amplicons were generated using Platinum Taq polymerase (Invitrogen) and pooled for each condition so that each sample contained all associated amplicons and used as input for NEBNext Ultra II DNA library prep, amplified with unique dual indexes to allow for multiplexing. Libraries were sequenced using an Illumina Nextseq 2000 instrument (2x150bp PE reads) to a read depth of at least 9 million read pairs per sample.

treatment_protocol: The cynomolgus monkey studies were conducted at Altasciences according to the IACUC guidelines. All monkeys were genotyped at the PCSK9 locus to ensure the DNA sequence at the sites where gRNAs bind have no mutations. Monkeys were randomly assigned to groups based on established social unit. Pretreatment medications of diphenhydramine (5 mg/kg), dexamethasone (1 mg/kg), and famotidine (0.5 mg/kg) were administrated to all monkeys via intramuscular injection one day and 30-60 min before the start of infusion. The LNP formulations or saline vehicle were administered by intravenous infusion for 1 hour. During the study, liver biopsy collections were performed under general anesthesia by ultrasonographic-guided percutaneous biopsy using a 16-gauge biopsy needle. Samples were flash frozen in liquid nitrogen, maintained on dry ice, then stored in a freezer set to maintain at -80°C.

tag: tissue

value: Liver

tag: treatment

value: Vehicle


title: Drosophila heads, repo-Gal4 control, sample 4

city: Leipzig

first: Diana

last: Le Duc

address: Philipp-Rosenthal-Str. 55

country: Germany

institute: Institute of Human Genetics,University of Leipzig Medical Center

library_source: transcriptomic

data_processing:
STAR,HTSEQ
Assembly: BDGP6.46 (GCA_000001215.4)

molecule: total RNA

organism: Drosophila melanogaster

source_name: adult heads

extract_protocol:
RNA was extracted using RNeasy Micro Kit (Qiagen) according to protocol (neuronal Bchs overexpression dataset) or exchanging the first step of homogenization in RLT buffer with the following Trizol protocol (glial Bchs overexpression dataset). Heads were homogenized in 500 µl Trizol, centrifuged for 5 min at 10000 rpm at 4°C, supernatant was tranferred to a new tube, 200 µl Chloroform (Carl Roth, No. 6340.1) was added and the tube was shaked. An incubation step at room temperature for 3 min was followed by centrifugation for 5 min at 10,000 rpm at 4°C. The upper aqueous phase was transferred in a new tube and it was continued with the RNeasy Micro Kit protocol.
RNA-seq libraries were prepared using TruSeq RNA Library Prep Kit v2 (Illumina, San Diego, CA).

value: adult heads

tag: genotype


title: Drosophila heads, repo-Gal4 control, sample 2


title: Drosophila heads, glial Bchs overexpression, sample 2


title: 0.5% CO2, pH 5.7, Rep 1,  part 2

city: East Lansing

first: Robert

last: Abramovitch

state: MI

address: 2215 Biomedical And Physical Sciences

institute: Michigan State University

data_processing:
Illumina bcl2fastq (v1.8.4) was used to de-multiplex and convert reads to FASTQ format
Reads from each lane were combined into individual files using standard UNIX commands
Trimmomatic (v0.33)  was used to trim low quality bases and remove adapter sequences with a 4 bp sliding window, cutting when the read quality dropped below 15 or read length was less than 36 bp.
Bowtie (v1.1.2) was used to align trimmed reads to the M. tuberculosis CDC1551 genome (NCBI accession AE000516) with the -S option to produce SAM files as output.
HTSeq software suite (v0.6.0) was used to analyze aligned and determine counts per gene feature in the M. tuberculosis CDC1551 genome
edgeR (v3.2) was used to normalize data by estimating effective library sizes using trimmed mean M-values
R was used for statistical analysis and differential gene expression by fitting a negative binomial model to each set of conditions and testing for differences utilizing the GLM functionality edgeR package
Assembly: M. tuberculosis CDC1551 genome (NCBI accession AE000516)
Supplementary files format and content: Processed files are presented as .xlsx spreadsheets and represents the average of 2 biological replicates

organism: Mycobacterium tuberculosis

source_name: Bacterial Cell

growth_protocol: M. tuberculosis CDC1551  was maintained in vented T-25 culture flasks in 7H9 Middlebrook medium supplemented with 10% oleic acid-albumin-dextrose-catalase (OADC), 0.05% Tween-80, and 0.2% glycerol and incubated at 37 °C with 5% CO2, unless noted otherwise. For experiments requiring buffered medium, 100 mM 3-(N-morpholino)propanesulfonic acid (MOPS) or 100 mM 2-(N-morpholino)ethanesulfonic acid (MES) was added to 7H9 medium for buffering to pH 7.0 or pH 5.7, respectively. Cultures were grown to mid-late log phase (OD600 0.5-1.0) for use in experiments described below.

extract_protocol:
RNA was extracted using the method Rohde, Abramovitch and Russell, Cell Host and Microbe, 2007. Briefly,  RNA was stabilized and isolated using a guanidine thiocyanate-based lysis buffer as previously described. Pelleted mycobacteria were lysed using 5 μg/ml lysozyme, hot Trizol, and physical disruption with silica beads in a BeadBeater. Total RNA was isolated by chloroform extraction followed by direct application to QIAGEN RNeasy column purification. Residual DNA contamination was removed using Turbo DNAfree DNase (Ambion).  RNA quality and integrity were examined using an Agilent Bioanalyzer prior to subjecting samples to rRNA depletion using the Epicentre Ribo-Zero depletion kit.
cDNA libraries were constructed using the Illumina TruSeq RNA library preparation kit (v2), omitting the polyA selection step. Multiplexing sequences are included in the title of the associated fastq.gz files.
After library quality control, sample libraries were pooled and sequenced in two lanes of an Illumina HiSeq 2500 Rapid Run flow cell (v1) in 50bp, single-end read format (SE50). After the sequencing run, reads were de-multiplexed and converted to FASTQ format using the Illumina bcl2fastq (v1.8.4) script.

treatment_protocol: M. tuberculosis CDC1551 ultures were seeded at a starting OD600 of 0.2  in 8 mL of 7H9 buffered media and grown at 37°C in standing T-25 culture flasks. Two biological replicates of the following culture conditions were examined: i)  0.5% CO2 at pH 5.7, ii)  5% CO2 at pH 5.7, , iii) 0.5% CO2 at pH 7.0, iv) 5% CO2 at pH 7.0. Cultures were incubated for six days, after which total bacterial RNA was extracted as previously described [1]

tag: strain

tag: condition


title: Lt8133 ChIP H3K4me3 2

middle: A

state: NY

department: Delbruck Bldg.

description: Library name: lt8133-h3k4me3-rep2

source_name: whole frond

growth_protocol: Sterile cultures were obtained from the Rutgers Duckweed Stock Cooperative and cultivated in 50 mL Schenk and Hildebrandt (SH) medium with 1% sucrose at pH 5.6 at 23°C under a 16 hour photoperiod of approximately 30 μmol/m2/s per second of white fluorescent light.

extract_protocol:
Chromatin extraction and immunoprecipitation was performed as previously described (Parent et al. 2021) with minor modifications. Briefly, two biological replicates of 750 mg whole frond tissue per clone were crosslinked in 1% formaldehyde for 10 min, and ground to a fine powder under liquid nitrogen. Nuclei were extracted in an SDS-Tris buffer, lysed, and sonicated with a Bioruptor Pico (Diagenode) for 12 cycles at 30 Hz, 30 s on, 30 s off.
CENH3 orthologues in S. polyrhiza, W. australiana, L. gibba, and L. japonica were identified by sequence similarity search with O. sativa and A. thaliana sequences. A rabbit polyclonal antibody was raised against the synthesized polypeptide sequence “RTKHISGKRRRTE” unique to the L. minor and L. turionifera CENH3 N-terminal tail (LjCENH3), and then validated by Western blot. Chromatin was cleared with Protein A magnetic beads (Thermo Fisher Scientific) and incubated overnight with following antibodies: LjCENH3 (GenScript), H3 (Sigma, H0164), and H3K4me3 (Sigma, 07-473). Immune complexes were eluted, and crosslinks were reversed with Proteinase K digestion for 2 hrs at 55C with gentle shaking. Overnight NaCl incubation was omitted as in 129. DNA was purified with the ChIP Purification Kit (Zymo), and the NEBNext Ultra II DNA library prep kit (New England Biolabs) was used to construct sequencing libraries. L. turionifera samples in these libraries were from the clone 8133, rather than 9434.


title: 0.5% CO2, pH 5.7, Rep 1, part 1


title: Lt8133 ChIP H3 1

description: Library name: lt8133-h3-rep1


title: deletion_NaCl_t15_A

city: Barcelona

first: Francesc

last: Posas

address: Carrar Baldiri Reixac 10

country: Spain

institute: Cell Signaling Group, IRB Barcelona

description: 10X genomics

library_source: transcriptomic single cell

data_processing:
Raw FASTQ files were processed with cellranger-4.0.0 to perform sample demultiplexing, cell barcode extraction and gene counting.
Assembly: Reference genome was obtained from (Nadal-Ribelles, Mariona, et al. "Control of Cdc28 CDK1 by a stress-induced lncRNA." Molecular cell 53.4 (2014): 549-561.)
Supplementary files format and content: Tab separated values files and matrix files

molecule: polyA RNA

organism: Saccharomyces cerevisiae

growth_protocol: Cells were grown individually in rich medium. At mid exponential growth phase cells were pooled at a 1:1 ratio  before treatment.

extract_protocol:
Fixed cells were rehydrated follwoing manufacturer's protocol
Library was performed according to manufacturer's protocol (10X v3.2) except for a minor modification, by which zymoliase was added to the enzyme mix to perform an in drop cell wall digestion.
scRNA-seq 3' end

treatment_protocol: Cells were treated or not (control) with osmotic stress 0.4M NaCl at the indicated times. Cells were harvested and fixed with methanol using 10X methanol fixation protocol.

tag: cell line

tag: group

value: deletion

value: MATa his3{delta}1 leu2{delta}0 met15{delta}0 ura3{delta}0

value: NaCl 0.4M

tag: time


title: Lt8133 ChIP Input 2

description: Library name: lt8133-input-rep2


title: deletion_NaCl_t15_B

description: 10X genomics


title: Drosophila heads, UAS-bchs::HAcontrol (glia dataset), sample 1


title: Lt8133 ChIP CENH3 2

description: Library name: lt8133-cenh3-rep2


title: Drosophila heads, glial Bchs overexpression, sample 1


title: deletion_control

description: 10X genomics

value: control


title: Lt8133 ChIP CENH3 1

description: Library name: lt8133-cenh3-rep1


title: Lt8133 ChIP Input 1

description: Library name: lt8133-input-rep1


title: 0.5% CO2, pH 5.7, Rep 2, part 1


title: Lt8133 ChIP H3K4me3 1

description: Library name: lt8133-h3k4me3-rep1


title: Drosophila heads, repo-Gal4 control, sample 1


title: Mock-36-PF573228-24

status: Public on Feb 03 2025

type: RNA

city: New York

first: Mariano

middle: Javier

last: Alvarez

address: 1130 St. Nicholas ave.

department: Systems Biology

institute: Columbia University

description:
Mouse EpiSC treated for 36h with NA Mock and for 24h with 250 nM PF573228
5003139004_B

hyb_protocol: cRNA is hybridized on mouseWG-6 v2 BeadArrays (Illumina)

scan_protocol: Slides are scanned using an iScan (Illumina, SY-101-1001)

data_processing:
Variance stabilization and robust spline normalization implemented in the lumi package for the R-System (PMID: 18467348)
Hybridization signal was normalized by variance stabilization and robust spline normalization implemented in the lumi package for the R-System (PMID: 18467348)

label: biotin

organism: Mus musculus

source_name: Epiblast Stem Cell

label_protocol: Total RNA was biotinlabelled using the Illumina TotalPrep RNA Amplification Kit.

growth_protocol: Cells are cultured in Matrigel coated plates with filtered MEF conditioned media (EpiSC basal media harvested after culturing with inactivated MEFs for 24h) supplemented with 12.5ng/ml bFGF and 10ng/ml Activin (CMAF).

extract_protocol: Cells were lysed with Trizol and total RNA was prepared with a column-based kit.

treatment_protocol: EpiSC were perturbed for 36h with 33 distinct small molecule compounds, and for 24h with 7 different differentiation morphogens or control culture conditions

tag: morphogen

value: Mock

tag: morphogen time

tag: drug

tag: drug concentration

tag: drug time

tag: cell type

value: Epiblast Stem Cell (EpiSC)


title: Mock-36-CM-24, rep1

description:
Mouse EpiSC treated for 36h with NA Mock and for 24h with NA CM
5003139004_F

value: CM


title: Mock-36-Dorsomorphin dihydrochloride-24

description:
Mouse EpiSC treated for 36h with NA Mock and for 24h with 2 uM Dorsomorphin dihydrochloride
5003139004_C

value: Dorsomorphin dihydrochloride

value: 2 uM


title: Retinoic acid-24-Purmorphamine-36

description:
Mouse EpiSC treated for 24h with 5 uM Retinoic acid and for 36h with 2 uM Purmorphamine
4825641026_D

value: Retinoic acid

tag: morphogen concentration

value: 5 uM

value: Purmorphamine


title: Retinoic acid-24-SU5402-36

description:
Mouse EpiSC treated for 24h with 5 uM Retinoic acid and for 36h with 2 uM SU5402
4825641026_E


title: Mock-36-Rapamycin-24

description:
Mouse EpiSC treated for 36h with NA Mock and for 24h with 100 nM Rapamycin
5003139004_A

value: Rapamycin


title: Mock-36-BAPTA-AM-24

description:
Mouse EpiSC treated for 36h with NA Mock and for 24h with 10 uM BAPTA-AM
5003139005_A

value: BAPTA-AM

value: 10 uM


title: Mock-36-BAY 11-7082-24

description:
Mouse EpiSC treated for 36h with NA Mock and for 24h with 25uM BAY 11-7082
5003139004_D


title: Mock-36-CM-24, rep2

description:
Mouse EpiSC treated for 36h with NA Mock and for 24h with NA CM
5003139005_F


title: Retinoic acid-24-Mock-36

description:
Mouse EpiSC treated for 24h with 5 uM Retinoic acid and for 36h with NA Mock
4825641027_F


title: Retinoic acid-24-Wortmannin-36

description:
Mouse EpiSC treated for 24h with 5 uM Retinoic acid and for 36h with 50 nM Wortmannin
4825641026_C

value: Wortmannin

value: 50 nM


title: Retinoic acid-24-Rapamycin-36

description:
Mouse EpiSC treated for 24h with 5 uM Retinoic acid and for 36h with 100 nM Rapamycin
4825641027_A


title: Mock-36-Forskolin-24

description:
Mouse EpiSC treated for 36h with NA Mock and for 24h with 50 uM Forskolin
5003139004_E

value: Forskolin

value: 50 uM


title: Mock-36-Purmorphamine-24

description:
Mouse EpiSC treated for 36h with NA Mock and for 24h with 2 uM Purmorphamine
5003139005_D


title: Retinoic acid-24-Dorsomorphin dihydrochloride-36

description:
Mouse EpiSC treated for 24h with 5 uM Retinoic acid and for 36h with 2 uM Dorsomorphin dihydrochloride
4825641027_C


title: Retinoic acid-36-SU5402-24

description:
Mouse EpiSC treated for 36h with 5 uM Retinoic acid and for 24h with 2 uM SU5402
4825641005_E


title: Mock-36-SU5402-24

description:
Mouse EpiSC treated for 36h with NA Mock and for 24h with 2 uM SU5402
5003139005_E


title: Retinoic acid-24-Docetaxel-36

description:
Mouse EpiSC treated for 24h with 5 uM Retinoic acid and for 36h with 500 pM Docetaxel
4825641026_B

value: Docetaxel


title: Retinoic acid-36-Wortmannin-24

description:
Mouse EpiSC treated for 36h with 5 uM Retinoic acid and for 24h with 50 nM Wortmannin
4825641005_C


title: Retinoic acid-24-PF573228-36

description:
Mouse EpiSC treated for 24h with 5 uM Retinoic acid and for 36h with 250 nM PF573228
4825641027_B


title: Retinoic acid-24-CM-36

description:
Mouse EpiSC treated for 24h with 5 uM Retinoic acid and for 36h with NA CM
4825641026_F


title: Mock-36-Docetaxel-24

description:
Mouse EpiSC treated for 36h with NA Mock and for 24h with 500 pM Docetaxel
5003139005_B


title: Retinoic acid-24-BAY 11-7082-36

description:
Mouse EpiSC treated for 24h with 5 uM Retinoic acid and for 36h with 25uM BAY 11-7082
4825641027_D


title: Mock-36-Mock-24

description:
Mouse EpiSC treated for 36h with NA Mock and for 24h with NA Mock
4825641005_F


title: Retinoic acid-24-Forskolin-36

description:
Mouse EpiSC treated for 24h with 5 uM Retinoic acid and for 36h with 50 uM Forskolin
4825641027_E


title: Retinoic acid-36-Purmorphamine-24

description:
Mouse EpiSC treated for 36h with 5 uM Retinoic acid and for 24h with 2 uM Purmorphamine
4825641005_D


title: Retinoic acid-24-BAPTA-AM-36

description:
Mouse EpiSC treated for 24h with 5 uM Retinoic acid and for 36h with 10 uM BAPTA-AM
4825641026_A


title: Mock-36-Wortmannin-24

description:
Mouse EpiSC treated for 36h with NA Mock and for 24h with 50 nM Wortmannin
5003139005_C


title: Retinoic acid-36-Mock-24

description:
Mouse EpiSC treated for 36h with 5 uM Retinoic acid and for 24h with NA Mock
4825641042_F


title: Retinoic acid-36-Dorsomorphin dihydrochloride-24

description:
Mouse EpiSC treated for 36h with 5 uM Retinoic acid and for 24h with 2 uM Dorsomorphin dihydrochloride
4825641042_C


title: Retinoic acid-36-Docetaxel-24

description:
Mouse EpiSC treated for 36h with 5 uM Retinoic acid and for 24h with 500 pM Docetaxel
4825641005_B


title: Retinoic acid-36-BAPTA-AM-24

description:
Mouse EpiSC treated for 36h with 5 uM Retinoic acid and for 24h with 10 uM BAPTA-AM
4825641005_A


title: EpiSC-5 None None 5426836071_F

description: Mouse EpiSC-5 treated for 24h with NA None and for 36h with NA None

data_processing: Variance stabilization and robust spline normalization implemented in the lumi package for the R-System (PMID: 18467348)

tag: batch


title: Retinoic acid-36-Forskolin-24

description:
Mouse EpiSC treated for 36h with 5 uM Retinoic acid and for 24h with 50 uM Forskolin
4825641042_E


title: EpiSC-5 None None 5426836003_C


title: EpiSC-5 None None 5426836102_E


title: EpiSC-5 None None 5426836102_D


title: EpiSC-5 None None 5426836072_F


title: Retinoic acid-36-Rapamycin-24

description:
Mouse EpiSC treated for 36h with 5 uM Retinoic acid and for 24h with 100 nM Rapamycin
4825641042_A


title: EpiSC-5 None None 5426836088_C


title: EpiSC-5 None None 5426836090_C


title: EpiSC-5 None None 5426836020_F


title: EpiSC-5 None None 5426836084_C


title: EpiSC-5 None None 5426836089_C


title: EpiSC-5 None None 5426836071_C


title: Retinoic acid-36-BAY 11-7082-24

description:
Mouse EpiSC treated for 36h with 5 uM Retinoic acid and for 24h with 25uM BAY 11-7082
4825641042_D


title: EpiSC-5 None None 5417796052_E


title: Retinoic acid-36-PF573228-24

description:
Mouse EpiSC treated for 36h with 5 uM Retinoic acid and for 24h with 250 nM PF573228
4825641042_B


title: EpiSC-5 None None 5417796052_D


title: EpiSC-5 None None 5426836081_D


title: EpiSC-5 None Purmorphamine 5417796004_E

description: Mouse EpiSC-5 treated for 24h with NA None and for 36h with 2 uM Purmorphamine


title: EpiSC-5 None None 5417796042_D


title: EpiSC-5 None PD0325901 5417796059_E

description: Mouse EpiSC-5 treated for 24h with NA None and for 36h with 1 uM PD0325901

value: 1 uM


title: EpiSC-5 SB431542 Rapamycin 5426836080_F

description: Mouse EpiSC-5 treated for 24h with 10 uM SB431542 and for 36h with 100 nM Rapamycin


title: EpiSC-5 SB431542 Wnt agonist 5426836094_F

description: Mouse EpiSC-5 treated for 24h with 10 uM SB431542 and for 36h with 150 nM Wnt agonist

value: Wnt agonist


title: EpiSC-5 Retinoic acid Wortmannin 5417796003_E

description: Mouse EpiSC-5 treated for 24h with 5 uM Retinoic acid and for 36h with 50 nM Wortmannin


title: EpiSC-5 None None 5417796052_F


title: EpiSC-5 SB431542 FGF2 5426836110_F

description: Mouse EpiSC-5 treated for 24h with 10 uM SB431542 and for 36h with 12.5ng/ml FGF2


title: EpiSC-5 SB431542 Wortmannin 5426836070_F

description: Mouse EpiSC-5 treated for 24h with 10 uM SB431542 and for 36h with 50 nM Wortmannin


title: EpiSC-5 Retinoic acid Rapamycin 5417796042_E

description: Mouse EpiSC-5 treated for 24h with 5 uM Retinoic acid and for 36h with 100 nM Rapamycin


title: EpiSC-5 SB431542 PD0325901 5426836090_F

description: Mouse EpiSC-5 treated for 24h with 10 uM SB431542 and for 36h with 1 uM PD0325901


title: EpiSC-5 SB431542 Genistein 5426836079_F

description: Mouse EpiSC-5 treated for 24h with 10 uM SB431542 and for 36h with 15 uM Genistein

value: Genistein

value: 15 uM


title: EpiSC-5 SB431542 Purmorphamine 5426836084_F

description: Mouse EpiSC-5 treated for 24h with 10 uM SB431542 and for 36h with 2 uM Purmorphamine


title: EpiSC-5 SB431542 Indolactam 5426836088_F

description: Mouse EpiSC-5 treated for 24h with 10 uM SB431542 and for 36h with 300 nM Indolactam

value: Indolactam


title: EpiSC-5 None BMP4 5426836081_E

description: Mouse EpiSC-5 treated for 24h with NA None and for 36h with 100ng/ml BMP4


title: EpiSC-5 None PD0325901 5417796059_F


title: EpiSC-5 None None 5426836101_F

description: Mouse EpiSC-5 treated for 24h with NA None and for 36h with NA None


title: EpiSC-5 None None 5417796097_E


title: EpiSC-5 Retinoic acid Indolactam 5417796001_E

description: Mouse EpiSC-5 treated for 24h with 5 uM Retinoic acid and for 36h with 300 nM Indolactam


title: EpiSC-5 Retinoic acid Genistein 5417796018_E

description: Mouse EpiSC-5 treated for 24h with 5 uM Retinoic acid and for 36h with 15 uM Genistein


title: EpiSC-5 SB431542 Y-27632 5426836100_F

description: Mouse EpiSC-5 treated for 24h with 10 uM SB431542 and for 36h with 10 uM Y-27632


title: EpiSC-5 SB431542 (5Z)-7-Oxozeaenol 5426836109_F

description: Mouse EpiSC-5 treated for 24h with 10 uM SB431542 and for 36h with 1 uM (5Z)-7-Oxozeaenol


title: EpiSC-5 Retinoic acid Y-27632 5417796073_E

description: Mouse EpiSC-5 treated for 24h with 5 uM Retinoic acid and for 36h with 10 uM Y-27632


title: EpiSC-5 SB431542 Dorsomorphin dihydrochloride 5426836089_F

description: Mouse EpiSC-5 treated for 24h with 10 uM SB431542 and for 36h with 2 uM Dorsomorphin dihydrochloride

value: Dorsomorphin dihydrochloride

value: 2 uM


title: EpiSC-5 None None 5417796097_F


title: EpiSC-5 Retinoic acid Dorsomorphin dihydrochloride 5426836095_E

description: Mouse EpiSC-5 treated for 24h with 5 uM Retinoic acid and for 36h with 2 uM Dorsomorphin dihydrochloride

value: Retinoic acid

value: 5 uM


title: EpiSC-5 Retinoic acid Wnt agonist 5417796014_E

description: Mouse EpiSC-5 treated for 24h with 5 uM Retinoic acid and for 36h with 150 nM Wnt agonist


title: EpiSC-5 Retinoic acid FGF2 5426836101_E

description: Mouse EpiSC-5 treated for 24h with 5 uM Retinoic acid and for 36h with 12.5ng/ml FGF2


title: EpiSC-5 SB431542 BIO-acetoxime 5426836096_F

description: Mouse EpiSC-5 treated for 24h with 10 uM SB431542 and for 36h with 5 uM BIO-acetoxime

value: BIO-acetoxime


title: EpiSC-5 Retinoic acid BIO-acetoxime 5417796016_E

description: Mouse EpiSC-5 treated for 24h with 5 uM Retinoic acid and for 36h with 5 uM BIO-acetoxime


title: EpiSC-5 PD0325901 Y-27632 5417796073_D

description: Mouse EpiSC-5 treated for 24h with 1 uM PD0325901 and for 36h with 10 uM Y-27632


title: EpiSC-5 Retinoic acid (5Z)-7-Oxozeaenol 5417796002_E

description: Mouse EpiSC-5 treated for 24h with 5 uM Retinoic acid and for 36h with 1 uM (5Z)-7-Oxozeaenol


title: EpiSC-5 PD0325901 FGF2 5426836101_D

description: Mouse EpiSC-5 treated for 24h with 1 uM PD0325901 and for 36h with 12.5ng/ml FGF2


title: EpiSC-5 PD0325901 Rapamycin 5426836095_D

description: Mouse EpiSC-5 treated for 24h with 1 uM PD0325901 and for 36h with 100 nM Rapamycin

value: Rapamycin


title: EpiSC-5 PD0325901 Genistein 5417796018_D

description: Mouse EpiSC-5 treated for 24h with 1 uM PD0325901 and for 36h with 15 uM Genistein


title: EpiSC-5 Embryod body Y-27632 5426836100_D

description: Mouse EpiSC-5 treated for 24h with NA Embryod body and for 36h with 10 uM Y-27632

value: Embryod body

value: 10 uM


title: EpiSC-5 Embryod body Dorsomorphin dihydrochloride 5426836089_D

description: Mouse EpiSC-5 treated for 24h with NA Embryod body and for 36h with 2 uM Dorsomorphin dihydrochloride


title: EpiSC-5 Embryod body Wnt agonist 5426836094_D

description: Mouse EpiSC-5 treated for 24h with NA Embryod body and for 36h with 150 nM Wnt agonist


title: EpiSC-5 Embryod body Genistein 5426836079_D

description: Mouse EpiSC-5 treated for 24h with NA Embryod body and for 36h with 15 uM Genistein


title: EpiSC-5 Embryod body None 5426836072_C

description: Mouse EpiSC-5 treated for 24h with NA Embryod body and for 36h with NA None


title: EpiSC-5 Embryod body Purmorphamine 5426836084_D

description: Mouse EpiSC-5 treated for 24h with NA Embryod body and for 36h with 2 uM Purmorphamine

value: Purmorphamine


title: EpiSC-5 Embryod body Retinoic acid 5426836071_D

description: Mouse EpiSC-5 treated for 24h with NA Embryod body and for 36h with 5 uM Retinoic acid


title: EpiSC-5 Embryod body PD0325901 5426836090_D

description: Mouse EpiSC-5 treated for 24h with NA Embryod body and for 36h with 1 uM PD0325901


title: EpiSC-5 Embryod body None 5426836020_D


title: EpiSC-5 Embryod body BIO-acetoxime 5426836096_D

description: Mouse EpiSC-5 treated for 24h with NA Embryod body and for 36h with 5 uM BIO-acetoxime


title: EpiSC-5 PD0325901 Dorsomorphin dihydrochloride 5417796004_D

description: Mouse EpiSC-5 treated for 24h with 1 uM PD0325901 and for 36h with 2 uM Dorsomorphin dihydrochloride


title: EpiSC-5 Embryod body Wortmannin 5426836070_D

description: Mouse EpiSC-5 treated for 24h with NA Embryod body and for 36h with 50 nM Wortmannin

value: Wortmannin

value: 50 nM


title: EpiSC-5 PD0325901 (5Z)-7-Oxozeaenol 5417796002_D

description: Mouse EpiSC-5 treated for 24h with 1 uM PD0325901 and for 36h with 1 uM (5Z)-7-Oxozeaenol


title: EpiSC-5 Embryod body (5Z)-7-Oxozeaenol 5426836109_D

description: Mouse EpiSC-5 treated for 24h with NA Embryod body and for 36h with 1 uM (5Z)-7-Oxozeaenol


title: EpiSC-5 Embryod body BMP4 5426836072_D

description: Mouse EpiSC-5 treated for 24h with NA Embryod body and for 36h with 100ng/ml BMP4


title: EpiSC-5 PD0325901 Indolactam 5417796001_D

description: Mouse EpiSC-5 treated for 24h with 1 uM PD0325901 and for 36h with 300 nM Indolactam


title: EpiSC-5 PD0325901 Purmorphamine 5417796059_D

description: Mouse EpiSC-5 treated for 24h with 1 uM PD0325901 and for 36h with 2 uM Purmorphamine


title: EpiSC-5 Embryod body None 5426836020_C


title: EpiSC-5 Embryod body IPA-3 5426836003_D

description: Mouse EpiSC-5 treated for 24h with NA Embryod body and for 36h with 20 uM IPA-3

value: 20 uM


title: EpiSC-5 Embryod body Rapamycin 5426836080_D

description: Mouse EpiSC-5 treated for 24h with NA Embryod body and for 36h with 100 nM Rapamycin


title: EpiSC-5 Embryod body FGF2 5426836110_D

description: Mouse EpiSC-5 treated for 24h with NA Embryod body and for 36h with 12.5ng/ml FGF2


title: EpiSC-5 CMAF None 5426836020_E

description: Mouse EpiSC-5 treated for 24h with NA CMAF and for 36h with NA None

value: CMAF


title: EpiSC-5 PD0325901 BIO-acetoxime 5417796016_D

description: Mouse EpiSC-5 treated for 24h with 1 uM PD0325901 and for 36h with 5 uM BIO-acetoxime

value: 1 uM


title: EpiSC-5 CMAF None 5426836003_F


title: EpiSC-5 CMAF None 5417796097_D


title: EpiSC-5 CMAF Y-27632 5426836100_E

description: Mouse EpiSC-5 treated for 24h with NA CMAF and for 36h with 10 uM Y-27632


title: EpiSC-5 CMAF Wortmannin 5426836070_E

description: Mouse EpiSC-5 treated for 24h with NA CMAF and for 36h with 50 nM Wortmannin


title: EpiSC-5 CMAF FGF2 5426836110_E

description: Mouse EpiSC-5 treated for 24h with NA CMAF and for 36h with 12.5ng/ml FGF2


title: EpiSC-5 CMAF IPA-3 5426836003_E

description: Mouse EpiSC-5 treated for 24h with NA CMAF and for 36h with 20 uM IPA-3


title: EpiSC-5 CMAF BIO-acetoxime 5426836096_E

description: Mouse EpiSC-5 treated for 24h with NA CMAF and for 36h with 5 uM BIO-acetoxime


title: EpiSC-5 CMAF Genistein 5426836079_E

description: Mouse EpiSC-5 treated for 24h with NA CMAF and for 36h with 15 uM Genistein

value: Genistein


title: EpiSC-5 BMP4 None 5426836102_F

description: Mouse EpiSC-5 treated for 24h with 100ng/ml BMP4 and for 36h with NA None


title: EpiSC-5 CMAF Purmorphamine 5426836084_E

description: Mouse EpiSC-5 treated for 24h with NA CMAF and for 36h with 2 uM Purmorphamine


title: EpiSC-5 CMAF BMP4 5426836072_E

description: Mouse EpiSC-5 treated for 24h with NA CMAF and for 36h with 100ng/ml BMP4


title: EpiSC-5 CMAF Indolactam 5426836088_E

description: Mouse EpiSC-5 treated for 24h with NA CMAF and for 36h with 300 nM Indolactam


title: EpiSC-5 CMAF Retinoic acid 5426836071_E

description: Mouse EpiSC-5 treated for 24h with NA CMAF and for 36h with 5 uM Retinoic acid


title: EpiSC-5 CMAF None 5417796003_D


title: EpiSC-5 CMAF Dorsomorphin dihydrochloride 5426836089_E

description: Mouse EpiSC-5 treated for 24h with NA CMAF and for 36h with 2 uM Dorsomorphin dihydrochloride


title: EpiSC-5 CMAF (5Z)-7-Oxozeaenol 5426836109_E

description: Mouse EpiSC-5 treated for 24h with NA CMAF and for 36h with 1 uM (5Z)-7-Oxozeaenol


title: EpiSC-5 CMAF PD0325901 5426836090_E

description: Mouse EpiSC-5 treated for 24h with NA CMAF and for 36h with 1 uM PD0325901


title: EpiSC-5 CMAF Wnt agonist 5426836094_E

description: Mouse EpiSC-5 treated for 24h with NA CMAF and for 36h with 150 nM Wnt agonist

value: Wnt agonist


title: EpiSC-5 BMP4 Y-27632 5417796073_F

description: Mouse EpiSC-5 treated for 24h with 100ng/ml BMP4 and for 36h with 10 uM Y-27632


title: EpiSC-5 BMP4 Indolactam 5417796001_F

description: Mouse EpiSC-5 treated for 24h with 100ng/ml BMP4 and for 36h with 300 nM Indolactam

value: Indolactam


title: EpiSC-5 BMP4 Wortmannin 5417796003_F

description: Mouse EpiSC-5 treated for 24h with 100ng/ml BMP4 and for 36h with 50 nM Wortmannin


title: EpiSC-5 PD0325901 Wnt agonist 5417796014_D

description: Mouse EpiSC-5 treated for 24h with 1 uM PD0325901 and for 36h with 150 nM Wnt agonist


title: EpiSC-5 BMP4 Genistein 5417796018_F

description: Mouse EpiSC-5 treated for 24h with 100ng/ml BMP4 and for 36h with 15 uM Genistein

value: 15 uM


title: EpiSC-5 BMP4 Purmorphamine 5417796004_F

description: Mouse EpiSC-5 treated for 24h with 100ng/ml BMP4 and for 36h with 2 uM Purmorphamine

value: 2 uM


title: EpiSC-5 BMP4 Wnt agonist 5417796014_F

description: Mouse EpiSC-5 treated for 24h with 100ng/ml BMP4 and for 36h with 150 nM Wnt agonist


title: EpiSC-5 BMP4 FGF2 5426836081_F

description: Mouse EpiSC-5 treated for 24h with 100ng/ml BMP4 and for 36h with 12.5ng/ml FGF2


title: EpiSC-5 BMP4 Dorsomorphin dihydrochloride 5426836095_F

description: Mouse EpiSC-5 treated for 24h with 100ng/ml BMP4 and for 36h with 2 uM Dorsomorphin dihydrochloride

value: Dorsomorphin dihydrochloride


title: EpiSC-5 BMP4 Rapamycin 5417796042_F

description: Mouse EpiSC-5 treated for 24h with 100ng/ml BMP4 and for 36h with 100 nM Rapamycin


title: EpiSC-5 BMP4 BIO-acetoxime 5417796016_F

description: Mouse EpiSC-5 treated for 24h with 100ng/ml BMP4 and for 36h with 5 uM BIO-acetoxime

value: 5 uM


title: EpiSC-G None None 5383338016_E

description: Mouse EpiSC-G treated for 24h with NA None and for 36h with NA None

value: EpiSC-G


title: EpiSC-G None PD0325901 5417796059_C

description: Mouse EpiSC-G treated for 24h with NA None and for 36h with 1 uM PD0325901


title: EpiSC-G None None 5383338058_E


title: EpiSC-G SB431542 None 5383338060_F

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with NA None


title: EpiSC-G None None 5426836070_C


title: EpiSC-G None None 5383338055_A


title: EpiSC-G None None 5426836096_C


title: EpiSC-G None PD0325901 5417796059_B


title: EpiSC-G None None 5417796021_E


title: EpiSC-G None None 5417796003_A


title: EpiSC-G None None 5426836102_B


title: EpiSC-G None None 5426836100_C


title: EpiSC-5 BMP4 (5Z)-7-Oxozeaenol 5417796002_F

description: Mouse EpiSC-5 treated for 24h with 100ng/ml BMP4 and for 36h with 1 uM (5Z)-7-Oxozeaenol


title: EpiSC-G None None 5426836021_E


title: EpiSC-G None None 5383338056_A


title: EpiSC-G None None 5426836080_C


title: EpiSC-G SB431542 None 5417796021_F


title: EpiSC-G SB431542 None 5383338059_C


title: EpiSC-G None None 5426836101_C


title: EpiSC-G SB431542 None 5383338058_F


title: EpiSC-G SB431542 Y-27632 5383338055_F

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 10 uM Y-27632


title: EpiSC-G None Purmorphamine 5417796004_B

description: Mouse EpiSC-G treated for 24h with NA None and for 36h with 2 uM Purmorphamine


title: EpiSC-G None BMP4 5426836081_B

description: Mouse EpiSC-G treated for 24h with NA None and for 36h with 100ng/ml BMP4


title: EpiSC-G SB431542 Y-27632 5383338025_C


title: EpiSC-G SB431542 Wortmannin 5383338036_F

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 50 nM Wortmannin


title: EpiSC-G SB431542 Wortmannin 5383338001_C


title: EpiSC-5 Embryod body Indolactam 5426836088_D

description: Mouse EpiSC-5 treated for 24h with NA Embryod body and for 36h with 300 nM Indolactam


title: EpiSC-G SB431542 Valproic acid 5383338016_F

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 1 mM Valproic acid

value: Valproic acid

value: 1 mM


title: EpiSC-G SB431542 Telomerase Inhibitor 3 5426836021_A

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 250 nM Telomerase Inhibitor 3

value: 10 uM

value: Telomerase Inhibitor 3


title: EpiSC-G SB431542 Telomerase Inhibitor 3 5383338016_A


title: EpiSC-G SB431542 Wnt agonist 5383338025_B

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 150 nM Wnt agonist


title: EpiSC-G SB431542 Retinoic acid 5426836021_C

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 5 uM Retinoic acid

value: Retinoic acid


title: EpiSC-G SB431542 Olomoucine 5383338059_B

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 20 uM Olomoucine

value: Olomoucine


title: EpiSC-G SB431542 STAT3 Inhibitor VIII, 5,15-DPP 5383338040_C

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 10 uM STAT3 Inhibitor VIII, 5,15-DPP

value: STAT3 Inhibitor VIII, 5,15-DPP


title: EpiSC-G SB431542 Purmorphamine 5383338025_F

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 2 uM Purmorphamine

value: Purmorphamine


title: EpiSC-G SB431542 PD0325901 5383338054_B

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 1 uM PD0325901

value: 1 uM


title: EpiSC-G SB431542 Purmorphamine 5383338054_C


title: EpiSC-G SB431542 LIF 5383338040_B

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 1000 unit LIF

value: LIF

value: 1000 unit


title: EpiSC-G SB431542 Valproic acid 5426836021_F


title: EpiSC-G SB431542 SB202190 5383338001_F

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 1 uM SB202190


title: EpiSC-G SB431542 Rapamycin 5383338018_E

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 100 nM Rapamycin

value: Rapamycin


title: EpiSC-G SB431542 SB202190 5383338055_C


title: EpiSC-G SB431542 STAT3 Inhibitor VIII, 5,15-DPP 5383338056_F


title: EpiSC-G SB431542 Olomoucine 5383338036_B

value: 20 uM


title: EpiSC-G SB431542 LDN-193189 5383338056_D

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 600 nM LDN-193189


title: EpiSC-G SB431542 LDN-193189 5383338040_A


title: EpiSC-G SB431542 LIF 5383338056_E


title: EpiSC-G SB431542 Locostatin 5383338036_A

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 30 uM Locostatin

value: Locostatin

value: 30 uM


title: EpiSC-G SB431542 Wnt agonist 5383338055_E

value: Wnt agonist


title: EpiSC-G SB431542 PD0325901 5383338025_E

value: EpiSC-G


title: EpiSC-G SB431542 SU5402 5383338018_D

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 2 uM SU5402

value: 2 uM


title: EpiSC-G SB431542 Retinoic acid 5383338016_C

value: 5 uM


title: EpiSC-G SB431542 Locostatin 5383338059_A

value: 10 uM


title: EpiSC-G SB431542 SU5402 5383338058_A


title: EpiSC-G SB431542 Rapamycin 5383338058_B


title: EpiSC-G SB431542 KT5720 5383338059_F

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 600 nM KT5720


title: EpiSC-G SB431542 IPA-3 5383338059_D

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 20 uM IPA-3


title: EpiSC-G SB431542 IWP-2 5383338054_A

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 1 uM IWP-2


title: EpiSC-G SB431542 IDE-1 5383338058_C

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 5 uM IDE-1


title: EpiSC-G SB431542 KAAD-Cyclopamine 5383338059_E

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 250 nM KAAD-Cyclopamine

value: KAAD-Cyclopamine


title: EpiSC-G SB431542 IPA-3 5383338001_D


title: EpiSC-G SB431542 Indolactam 5383338001_A

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 300 nM Indolactam

value: Indolactam


title: EpiSC-G SB431542 IDE-1 5383338018_F


title: EpiSC-G SB431542 Genistein 5383338036_E

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 15 uM Genistein

value: Genistein

value: 15 uM


title: EpiSC-G SB431542 KAAD-Cyclopamine 5383338054_D


title: EpiSC-G SB431542 KT5720 5383338036_C


title: EpiSC-G SB431542 Docetaxel 5426836021_D

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 500 pM Docetaxel

value: Docetaxel


title: EpiSC-G SB431542 Forskolin 5383338040_F

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 50 uM Forskolin

value: Forskolin

value: 50 uM


title: EpiSC-G SB431542 FGF2 5417796021_D

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 12.5ng/ml FGF2


title: EpiSC-G SB431542 Dexamethasone 5383338016_B

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 10 nM Dexamethasone

value: Dexamethasone

value: 10 nM


title: EpiSC-G SB431542 FGF2 5383338060_D


title: EpiSC-G SB431542 Dexamethasone 5426836021_B


title: EpiSC-G SB431542 CHIR99021 5383338025_A

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 3 uM CHIR99021

value: 3 uM


title: EpiSC-G SB431542 Genistein 5383338001_B


title: EpiSC-G SB431542 Forskolin 5383338018_C


title: EpiSC-G SB431542 BMP4 5383338060_C

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 100ng/ml BMP4


title: EpiSC-G SB431542 BMP4 5417796021_C


title: EpiSC-G SB431542 DAPT Secretase Inhibitor IX 5383338056_B

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 2.5 uM DAPT Secretase Inhibitor IX

value: DAPT Secretase Inhibitor IX


title: EpiSC-G SB431542 Dorsomorphin dihydrochloride 5383338054_F

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 2 uM Dorsomorphin dihydrochloride

value: Dorsomorphin dihydrochloride


title: EpiSC-5 CMAF Rapamycin 5426836080_E

description: Mouse EpiSC-5 treated for 24h with NA CMAF and for 36h with 100 nM Rapamycin

value: CMAF

value: Rapamycin


title: EpiSC-G SB431542 Docetaxel 5383338016_D

value: EpiSC-G


title: EpiSC-G SB431542 Dorsomorphin dihydrochloride 5383338056_C

value: 2 uM


title: EpiSC-G SB431542 DAPT Secretase Inhibitor IX 5383338054_E

value: 10 uM


title: EpiSC-G SB431542 BIO-acetoxime 5383338060_B

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 5 uM BIO-acetoxime

value: BIO-acetoxime

value: 5 uM


title: EpiSC-G SB431542 Bix01294 5417796021_A

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 1 uM Bix01294

value: 1 uM


title: EpiSC-G SB431542 BIO-acetoxime 5417796021_B


title: EpiSC-G SB431542 BAY 11-7082 5383338040_D

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 25uM BAY 11-7082


title: EpiSC-G SB431542 CHIR99021 5383338055_D


title: EpiSC-G SB431542 10058-F4 5383338040_E

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 25uM 10058-F4


title: EpiSC-G SB431542 Bix01294 5383338060_A


title: EpiSC-G SB431542 10058-F4 5383338018_B


title: EpiSC-G SB431542 (5Z)-7-Oxozeaenol 5383338055_B

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 1 uM (5Z)-7-Oxozeaenol


title: EpiSC-G Retinoic acid Wortmannin 5417796003_B

description: Mouse EpiSC-G treated for 24h with 5 uM Retinoic acid and for 36h with 50 nM Wortmannin

value: Retinoic acid

value: Wortmannin

value: 50 nM


title: EpiSC-G Retinoic acid Wnt agonist 5417796014_B

description: Mouse EpiSC-G treated for 24h with 5 uM Retinoic acid and for 36h with 150 nM Wnt agonist

value: Wnt agonist


title: EpiSC-G Retinoic acid None 5426836102_A

description: Mouse EpiSC-G treated for 24h with 5 uM Retinoic acid and for 36h with NA None


title: EpiSC-G Retinoic acid Y-27632 5417796073_B

description: Mouse EpiSC-G treated for 24h with 5 uM Retinoic acid and for 36h with 10 uM Y-27632


title: EpiSC-G Retinoic acid IPA-3 5417796097_B

description: Mouse EpiSC-G treated for 24h with 5 uM Retinoic acid and for 36h with 20 uM IPA-3

value: 20 uM


title: EpiSC-G Retinoic acid None 5426836081_A


title: EpiSC-G SB431542 BAY 11-7082 5383338018_A


title: EpiSC-G Retinoic acid Rapamycin 5417796042_B

description: Mouse EpiSC-G treated for 24h with 5 uM Retinoic acid and for 36h with 100 nM Rapamycin


title: EpiSC-G SB431542 (5Z)-7-Oxozeaenol 5383338001_E


title: EpiSC-G Retinoic acid Genistein 5417796018_B

description: Mouse EpiSC-G treated for 24h with 5 uM Retinoic acid and for 36h with 15 uM Genistein

value: Genistein

value: 15 uM


title: EpiSC-G Retinoic acid Indolactam 5417796001_B

description: Mouse EpiSC-G treated for 24h with 5 uM Retinoic acid and for 36h with 300 nM Indolactam

value: Indolactam


title: EpiSC-G Retinoic acid Dorsomorphin dihydrochloride 5426836095_B

description: Mouse EpiSC-G treated for 24h with 5 uM Retinoic acid and for 36h with 2 uM Dorsomorphin dihydrochloride

value: Dorsomorphin dihydrochloride


title: EpiSC-G Retinoic acid FGF2 5426836101_B

description: Mouse EpiSC-G treated for 24h with 5 uM Retinoic acid and for 36h with 12.5ng/ml FGF2


title: EpiSC-G Retinoic acid BIO-acetoxime 5417796016_B

description: Mouse EpiSC-G treated for 24h with 5 uM Retinoic acid and for 36h with 5 uM BIO-acetoxime


title: EpiSC-G PD0325901 None 5417796052_A

description: Mouse EpiSC-G treated for 24h with 1 uM PD0325901 and for 36h with NA None


title: EpiSC-G Retinoic acid None 5417796052_B


title: EpiSC-G PD0325901 None 5417796042_A


title: EpiSC-G SB431542 Indolactam 5383338036_D

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 300 nM Indolactam


title: EpiSC-G PD0325901 Rapamycin 5426836095_A

description: Mouse EpiSC-G treated for 24h with 1 uM PD0325901 and for 36h with 100 nM Rapamycin


title: EpiSC-G PD0325901 IPA-3 5417796097_A

description: Mouse EpiSC-G treated for 24h with 1 uM PD0325901 and for 36h with 20 uM IPA-3


title: EpiSC-G PD0325901 Y-27632 5417796073_A

description: Mouse EpiSC-G treated for 24h with 1 uM PD0325901 and for 36h with 10 uM Y-27632


title: EpiSC-G PD0325901 Wnt agonist 5417796014_A

description: Mouse EpiSC-G treated for 24h with 1 uM PD0325901 and for 36h with 150 nM Wnt agonist


title: EpiSC-G PD0325901 Genistein 5417796018_A

description: Mouse EpiSC-G treated for 24h with 1 uM PD0325901 and for 36h with 15 uM Genistein


title: EpiSC-G PD0325901 Purmorphamine 5417796059_A

description: Mouse EpiSC-G treated for 24h with 1 uM PD0325901 and for 36h with 2 uM Purmorphamine

value: Purmorphamine


title: EpiSC-G PD0325901 FGF2 5426836101_A

description: Mouse EpiSC-G treated for 24h with 1 uM PD0325901 and for 36h with 12.5ng/ml FGF2


title: EpiSC-G PD0325901 Dorsomorphin dihydrochloride 5417796004_A

description: Mouse EpiSC-G treated for 24h with 1 uM PD0325901 and for 36h with 2 uM Dorsomorphin dihydrochloride


title: EpiSC-G Embryod body None 5426836109_C

description: Mouse EpiSC-G treated for 24h with NA Embryod body and for 36h with NA None

value: Embryod body


title: EpiSC-G Retinoic acid (5Z)-7-Oxozeaenol 5417796002_B

description: Mouse EpiSC-G treated for 24h with 5 uM Retinoic acid and for 36h with 1 uM (5Z)-7-Oxozeaenol

value: EpiSC-G


title: EpiSC-G PD0325901 BIO-acetoxime 5417796016_A

description: Mouse EpiSC-G treated for 24h with 1 uM PD0325901 and for 36h with 5 uM BIO-acetoxime


title: EpiSC-G Embryod body None 5426836020_A


title: EpiSC-G Embryod body None 5417796001_A


title: EpiSC-G Embryod body None 5426836094_C


title: EpiSC-G Embryod body Wortmannin 5426836070_A

description: Mouse EpiSC-G treated for 24h with NA Embryod body and for 36h with 50 nM Wortmannin


title: EpiSC-G Embryod body Wnt agonist 5426836094_A

description: Mouse EpiSC-G treated for 24h with NA Embryod body and for 36h with 150 nM Wnt agonist


title: EpiSC-G Embryod body Retinoic acid 5426836071_A

description: Mouse EpiSC-G treated for 24h with NA Embryod body and for 36h with 5 uM Retinoic acid


title: EpiSC-G Embryod body None 5426836110_C


title: EpiSC-G Embryod body Rapamycin 5426836080_A

description: Mouse EpiSC-G treated for 24h with NA Embryod body and for 36h with 100 nM Rapamycin

value: Rapamycin


title: EpiSC-G Embryod body Y-27632 5426836100_A

description: Mouse EpiSC-G treated for 24h with NA Embryod body and for 36h with 10 uM Y-27632

value: 10 uM


title: EpiSC-G Embryod body Purmorphamine 5426836084_A

description: Mouse EpiSC-G treated for 24h with NA Embryod body and for 36h with 2 uM Purmorphamine

value: 2 uM


title: EpiSC-G Embryod body PD0325901 5426836090_A

description: Mouse EpiSC-G treated for 24h with NA Embryod body and for 36h with 1 uM PD0325901


title: EpiSC-G PD0325901 (5Z)-7-Oxozeaenol 5417796002_A

description: Mouse EpiSC-G treated for 24h with 1 uM PD0325901 and for 36h with 1 uM (5Z)-7-Oxozeaenol


title: EpiSC-G Embryod body Genistein 5426836079_A

description: Mouse EpiSC-G treated for 24h with NA Embryod body and for 36h with 15 uM Genistein


title: EpiSC-G Embryod body IPA-3 5426836003_A

description: Mouse EpiSC-G treated for 24h with NA Embryod body and for 36h with 20 uM IPA-3


title: EpiSC-G Embryod body BIO-acetoxime 5426836096_A

description: Mouse EpiSC-G treated for 24h with NA Embryod body and for 36h with 5 uM BIO-acetoxime

value: BIO-acetoxime

value: 5 uM


title: EpiSC-G Embryod body Indolactam 5426836088_A

description: Mouse EpiSC-G treated for 24h with NA Embryod body and for 36h with 300 nM Indolactam


title: EpiSC-G CMAF None 5426836079_C

description: Mouse EpiSC-G treated for 24h with NA CMAF and for 36h with NA None

value: CMAF


title: EpiSC-G Embryod body FGF2 5426836110_A

description: Mouse EpiSC-G treated for 24h with NA Embryod body and for 36h with 12.5ng/ml FGF2


title: EpiSC-G Embryod body Dorsomorphin dihydrochloride 5426836089_A

description: Mouse EpiSC-G treated for 24h with NA Embryod body and for 36h with 2 uM Dorsomorphin dihydrochloride


title: EpiSC-G SB431542 IWP-2 5383338025_D

description: Mouse EpiSC-G treated for 24h with 10 uM SB431542 and for 36h with 1 uM IWP-2

value: 1 uM


title: EpiSC-G CMAF Y-27632 5426836100_B

description: Mouse EpiSC-G treated for 24h with NA CMAF and for 36h with 10 uM Y-27632


title: EpiSC-G CMAF None 5426836020_B


title: EpiSC-G Embryod body BMP4 5426836072_A

description: Mouse EpiSC-G treated for 24h with NA Embryod body and for 36h with 100ng/ml BMP4


title: EpiSC-G CMAF None 5383338060_E


title: EpiSC-G CMAF Wortmannin 5426836070_B

description: Mouse EpiSC-G treated for 24h with NA CMAF and for 36h with 50 nM Wortmannin

value: Wortmannin

value: 50 nM


title: EpiSC-G CMAF Wnt agonist 5426836094_B

description: Mouse EpiSC-G treated for 24h with NA CMAF and for 36h with 150 nM Wnt agonist

value: Wnt agonist


title: EpiSC-G Embryod body (5Z)-7-Oxozeaenol 5426836109_A

description: Mouse EpiSC-G treated for 24h with NA Embryod body and for 36h with 1 uM (5Z)-7-Oxozeaenol


title: EpiSC-G CMAF Retinoic acid 5426836071_B

description: Mouse EpiSC-G treated for 24h with NA CMAF and for 36h with 5 uM Retinoic acid

value: Retinoic acid


title: EpiSC-G CMAF None 5383338058_D


title: EpiSC-G CMAF IPA-3 5426836003_B

description: Mouse EpiSC-G treated for 24h with NA CMAF and for 36h with 20 uM IPA-3

value: 20 uM


title: EpiSC-G CMAF Rapamycin 5426836080_B

description: Mouse EpiSC-G treated for 24h with NA CMAF and for 36h with 100 nM Rapamycin


title: EpiSC-G CMAF BIO-acetoxime 5426836096_B

description: Mouse EpiSC-G treated for 24h with NA CMAF and for 36h with 5 uM BIO-acetoxime


title: EpiSC-G BMP4 None 5426836102_C

description: Mouse EpiSC-G treated for 24h with 100ng/ml BMP4 and for 36h with NA None


title: EpiSC-G BMP4 Rapamycin 5417796042_C

description: Mouse EpiSC-G treated for 24h with 100ng/ml BMP4 and for 36h with 100 nM Rapamycin


title: EpiSC-G CMAF FGF2 5426836110_B

description: Mouse EpiSC-G treated for 24h with NA CMAF and for 36h with 12.5ng/ml FGF2


title: EpiSC-G CMAF Dorsomorphin dihydrochloride 5426836089_B

description: Mouse EpiSC-G treated for 24h with NA CMAF and for 36h with 2 uM Dorsomorphin dihydrochloride


title: EpiSC-G BMP4 Wortmannin 5417796003_C

description: Mouse EpiSC-G treated for 24h with 100ng/ml BMP4 and for 36h with 50 nM Wortmannin


title: EpiSC-G CMAF Indolactam 5426836088_B

description: Mouse EpiSC-G treated for 24h with NA CMAF and for 36h with 300 nM Indolactam

value: Indolactam


title: EpiSC-G CMAF BMP4 5426836072_B

description: Mouse EpiSC-G treated for 24h with NA CMAF and for 36h with 100ng/ml BMP4


title: EpiSC-G BMP4 IPA-3 5417796097_C

description: Mouse EpiSC-G treated for 24h with 100ng/ml BMP4 and for 36h with 20 uM IPA-3


title: EpiSC-G CMAF Genistein 5426836079_B

description: Mouse EpiSC-G treated for 24h with NA CMAF and for 36h with 15 uM Genistein

value: Genistein

value: 15 uM


title: EpiSC-G BMP4 Indolactam 5417796001_C

description: Mouse EpiSC-G treated for 24h with 100ng/ml BMP4 and for 36h with 300 nM Indolactam


title: EpiSC-G BMP4 BIO-acetoxime 5417796016_C

description: Mouse EpiSC-G treated for 24h with 100ng/ml BMP4 and for 36h with 5 uM BIO-acetoxime


title: EpiSC-G CMAF PD0325901 5426836090_B

description: Mouse EpiSC-G treated for 24h with NA CMAF and for 36h with 1 uM PD0325901


title: EpiSC-G BMP4 Genistein 5417796018_C

description: Mouse EpiSC-G treated for 24h with 100ng/ml BMP4 and for 36h with 15 uM Genistein


title: EpiSC-G CMAF (5Z)-7-Oxozeaenol 5426836109_B

description: Mouse EpiSC-G treated for 24h with NA CMAF and for 36h with 1 uM (5Z)-7-Oxozeaenol


title: EpiSC-G BMP4 Purmorphamine 5417796004_C

description: Mouse EpiSC-G treated for 24h with 100ng/ml BMP4 and for 36h with 2 uM Purmorphamine

value: Purmorphamine


title: R1_Activin-A_PF573228 [ME093]

data_processing:
Sequencing data was mapped to the mouse genome MGSCv37 with TopHat v1.3.3 (PMID: 19289445).
Reads mapping to known genes, based on Entrez gene identifiers, were then counted using the GenomicFeatures R-system package available from Bioconductor (PMID: 15461798).
Assembly: mm9 (MGSCv37)
Supplementary files format and content: Raw-counts per known gene using EntrezID gene identifiers

source_name: Mouse ES cells, R1 strain, perturbed for 30h with PF573228 and for 24h with Activin A

growth_protocol: Cells of both strains were maintained feeder-independently in N2B27 medium supplemented with 1uM PD184352 (Biovision, #1585-5), 3uM CHIR99021 (Sigma, #A3734), and 2uM SU5402 (Tocris Bioscience, #3300).

extract_protocol:
Cells grown on 6-well plates are lysed with Trizol (ThermoFisher, 15596018), and transferred into Eppendorf tubes for short-term storage at -80 degree. 600ul of cell lysate is used for total RNA extraction using column-based kit (Clontech, 740955).
mRNA is enriched by poly-A pull down, and library is synthesized using Illumina TruSeq RNA prep kit (Illumina, RS-122-2001). Libraries are pooled and sequenced using Illumina HiSeq2500 or 4000 platform, yielding approximately 30 million single-ended reads per sample.

treatment_protocol: mESC cells were treated for 30h with small molecule compounds at the indicated concentratios and for 24h with differentiation morphogens are the indicated concentrations.

value: mESC

value: Activin A

value: 20 ng/ml


title: EpiSC-G BMP4 FGF2 5426836081_C

description: Mouse EpiSC-G treated for 24h with 100ng/ml BMP4 and for 36h with 12.5ng/ml FGF2

value: EpiSC-G


title: EpiSC-G BMP4 Y-27632 5417796073_C

description: Mouse EpiSC-G treated for 24h with 100ng/ml BMP4 and for 36h with 10 uM Y-27632

value: 10 uM


title: EpiSC-G CMAF Purmorphamine 5426836084_B

description: Mouse EpiSC-G treated for 24h with NA CMAF and for 36h with 2 uM Purmorphamine

value: 2 uM


title: EpiSC-G BMP4 (5Z)-7-Oxozeaenol 5417796002_C

description: Mouse EpiSC-G treated for 24h with 100ng/ml BMP4 and for 36h with 1 uM (5Z)-7-Oxozeaenol


title: EpiSC-G BMP4 None 5417796052_C

description: Mouse EpiSC-G treated for 24h with 100ng/ml BMP4 and for 36h with NA None


title: EpiSC-G BMP4 Wnt agonist 5417796014_C

description: Mouse EpiSC-G treated for 24h with 100ng/ml BMP4 and for 36h with 150 nM Wnt agonist


title: R1_Activin-A_PD173074 [ME087]

source_name: Mouse ES cells, R1 strain, perturbed for 30h with PD173074 and for 24h with Activin A


title: R1_Activin-A_Olomoucine [ME080]

source_name: Mouse ES cells, R1 strain, perturbed for 30h with Olomoucine and for 24h with Activin A

value: Olomoucine

value: 30 uM


title: R1_Activin-A_KAAD-Cyclopamine [ME079]

source_name: Mouse ES cells, R1 strain, perturbed for 30h with KAAD-Cyclopamine and for 24h with Activin A

value: KAAD-Cyclopamine


title: R1_Activin-A_SB202190 [ME084]

source_name: Mouse ES cells, R1 strain, perturbed for 30h with SB202190 and for 24h with Activin A

value: 1 uM


title: R1_Activin-A_Genistein [ME088]

source_name: Mouse ES cells, R1 strain, perturbed for 30h with Genistein and for 24h with Activin A


title: EpiSC-G BMP4 Dorsomorphin dihydrochloride 5426836095_C

description: Mouse EpiSC-G treated for 24h with 100ng/ml BMP4 and for 36h with 2 uM Dorsomorphin dihydrochloride

value: Dorsomorphin dihydrochloride


title: R1_Activin-A_LDN-193189 [ME082]

source_name: Mouse ES cells, R1 strain, perturbed for 30h with LDN-193189 and for 24h with Activin A


title: R1_Activin-A_BIO [ME075]

source_name: Mouse ES cells, R1 strain, perturbed for 30h with BIO and for 24h with Activin A

value: BIO


title: R1_Activin-A_Docetaxel [ME090]

source_name: Mouse ES cells, R1 strain, perturbed for 30h with Docetaxel and for 24h with Activin A

value: Docetaxel


title: R1_Activin-A_CHIR99021 [ME094]

source_name: Mouse ES cells, R1 strain, perturbed for 30h with CHIR99021 and for 24h with Activin A

value: 3 uM


title: R1_Activin-A_DAPT,-gamma-secretase-inhibitor-IX [ME085]

source_name: Mouse ES cells, R1 strain, perturbed for 30h with DAPT, gamma secretase inhibitor IX and for 24h with Activin A

value: DAPT, gamma secretase inhibitor IX


title: R1_Activin-A_BAY-11-7082 [ME073]

source_name: Mouse ES cells, R1 strain, perturbed for 30h with BAY 11-7082 and for 24h with Activin A

value: 20 uM


title: JM8.N4_Retinoic-acid_Valproic-Acid [ME124]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Valproic Acid and for 24h with Retinoic acid

value: Valproic Acid

value: 25 uM

value: Retinoic acid

value: 5 uM


title: JM8.N4_Retinoic-acid_Wortmannin [ME139]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Wortmannin and for 24h with Retinoic acid

value: mESC

value: Wortmannin

value: 80 nM


title: JM8.N4_Retinoic-acid_SU5402 [ME134]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with SU5402 and for 24h with Retinoic acid

value: 2 uM


title: R1_Activin-A_10058-F4 [ME077]

source_name: Mouse ES cells, R1 strain, perturbed for 30h with 10058-F4 and for 24h with Activin A

value: Activin A

value: 20 ng/ml


title: JM8.N4_Retinoic-acid_Purmorphamine [ME144]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Purmorphamine and for 24h with Retinoic acid

value: Purmorphamine


title: JM8.N4_Retinoic-acid_PD173074 [ME135]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with PD173074 and for 24h with Retinoic acid


title: JM8.N4_Retinoic-acid_PF573228 [ME141]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with PF573228 and for 24h with Retinoic acid


title: JM8.N4_Retinoic-acid_LDN-193189 [ME130]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with LDN-193189 and for 24h with Retinoic acid


title: JM8.N4_Retinoic-acid_Olomoucine [ME128]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Olomoucine and for 24h with Retinoic acid

value: Olomoucine

value: 30 uM


title: JM8.N4_Retinoic-acid_KAAD-Cyclopamine [ME127]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with KAAD-Cyclopamine and for 24h with Retinoic acid

value: KAAD-Cyclopamine


title: JM8.N4_Retinoic-acid_DAPT,-gamma-secretase-inhibitor-IX [ME133]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with DAPT, gamma secretase inhibitor IX and for 24h with Retinoic acid


title: JM8.N4_Retinoic-acid_SB202190 [ME132]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with SB202190 and for 24h with Retinoic acid

value: 1 uM


title: JM8.N4_Retinoic-acid_Bix01294 [ME137]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Bix01294 and for 24h with Retinoic acid


title: JM8.N4_Retinoic-acid_Genistein [ME136]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Genistein and for 24h with Retinoic acid

value: Genistein

value: 10 uM


title: JM8.N4_Retinoic-acid_BIO [ME123]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with BIO and for 24h with Retinoic acid

value: BIO


title: JM8.N4_Retinoic-acid_CHIR99021 [ME142]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with CHIR99021 and for 24h with Retinoic acid

value: 3 uM


title: JM8.N4_Retinoic-acid_BAY-11-7082 [ME121]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with BAY 11-7082 and for 24h with Retinoic acid

value: 20 uM

value: Retinoic acid


title: JM8.N4_Retinoic-acid_BAPTA-AM [ME143]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with BAPTA-AM and for 24h with Retinoic acid

value: mESC

value: BAPTA-AM

value: 4 uM

value: 5 uM


title: JM8.N4_N2B27_Valproic-Acid [ME220]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Valproic Acid and for 24h with N2B27

value: Valproic Acid

value: 25 uM


title: R1_Activin-A_BAPTA-AM [ME095]

source_name: Mouse ES cells, R1 strain, perturbed for 30h with BAPTA-AM and for 24h with Activin A

value: Activin A

value: 20 ng/ml


title: JM8.N4_N2B27_PD173074 [ME231]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with PD173074 and for 24h with N2B27


title: JM8.N4_Retinoic-acid_Wnt-agonist [ME140]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Wnt agonist and for 24h with Retinoic acid

value: Wnt agonist

value: 40 nM


title: JM8.N4_N2B27_Purmorphamine [ME240]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Purmorphamine and for 24h with N2B27

value: Purmorphamine


title: JM8.N4_N2B27_SB202190 [ME228]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with SB202190 and for 24h with N2B27

value: 1 uM


title: JM8.N4_N2B27_Genistein [ME232]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Genistein and for 24h with N2B27

value: Genistein

value: 10 uM


title: R1_Activin-A_Dorsomorphin-dihydrochloride [ME078]

source_name: Mouse ES cells, R1 strain, perturbed for 30h with Dorsomorphin dihydrochloride and for 24h with Activin A

value: Dorsomorphin dihydrochloride


title: JM8.N4_N2B27_Olomoucine [ME224]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Olomoucine and for 24h with N2B27

value: Olomoucine

value: 30 uM


title: JM8.N4_N2B27_LDN-193189 [ME226]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with LDN-193189 and for 24h with N2B27

value: mESC


title: JM8.N4_N2B27_DAPT,-gamma-secretase-inhibitor-IX [ME229]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with DAPT, gamma secretase inhibitor IX and for 24h with N2B27

value: DAPT, gamma secretase inhibitor IX


title: JM8.N4_N2B27_BIO [ME219]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with BIO and for 24h with N2B27

value: BIO


title: JM8.N4_BMP4_Wortmannin [ME163]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Wortmannin and for 24h with BMP4

value: Wortmannin

value: 80 nM

value: 2 ng/ml


title: JM8.N4_N2B27_BAPTA-AM [ME239]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with BAPTA-AM and for 24h with N2B27

value: BAPTA-AM

value: 4 uM


title: JM8.N4_BMP4_Valproic-Acid [ME148]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Valproic Acid and for 24h with BMP4

value: Valproic Acid

value: 25 uM


title: JM8.N4_BMP4_Wnt-agonist [ME164]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Wnt agonist and for 24h with BMP4

value: Wnt agonist

value: 40 nM


title: JM8.N4_N2B27_BAY-11-7082 [ME217]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with BAY 11-7082 and for 24h with N2B27

value: 20 uM


title: JM8.N4_BMP4_Src-Inhibitor-II [ME153]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Src Inhibitor II and for 24h with BMP4

value: Src Inhibitor II

value: 10 uM


title: JM8.N4_BMP4_LDN-193189 [ME154]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with LDN-193189 and for 24h with BMP4


title: JM8.N4_N2B27_Wortmannin [ME235]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Wortmannin and for 24h with N2B27


title: JM8.N4_BMP4_Purmorphamine [ME168]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Purmorphamine and for 24h with BMP4

value: Purmorphamine


title: JM8.N4_BMP4_Genistein [ME160]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Genistein and for 24h with BMP4

value: Genistein


title: JM8.N4_BMP4_SB202190 [ME156]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with SB202190 and for 24h with BMP4

value: mESC

value: 1 uM


title: JM8.N4_BMP4_Docetaxel [ME162]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Docetaxel and for 24h with BMP4

value: Docetaxel


title: JM8.N4_BMP4_DAPT,-gamma-secretase-inhibitor-IX [ME157]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with DAPT, gamma secretase inhibitor IX and for 24h with BMP4

value: DAPT, gamma secretase inhibitor IX


title: JM8.N4_BMP4_BIO [ME147]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with BIO and for 24h with BMP4

value: BIO


title: JM8.N4_BMP4_CHIR99021 [ME166]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with CHIR99021 and for 24h with BMP4

value: 3 uM

value: 2 ng/ml


title: JM8.N4_BMP4_10058-F4 [ME149]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with 10058-F4 and for 24h with BMP4


title: JM8.N4_N2B27_CHIR99021 [ME238]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with CHIR99021 and for 24h with N2B27


title: JM8.N4_bFGF_Wnt-agonist [ME188]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Wnt agonist and for 24h with bFGF

value: bFGF

value: 6 ng/ml


title: JM8.N4_bFGF_Valproic-Acid [ME172]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Valproic Acid and for 24h with bFGF

value: Valproic Acid

value: 25 uM


title: JM8.N4_BMP4_BAPTA-AM [ME167]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with BAPTA-AM and for 24h with BMP4

value: BAPTA-AM

value: 4 uM


title: JM8.N4_N2B27_Bix01294 [ME233]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Bix01294 and for 24h with N2B27


title: JM8.N4_bFGF_Wortmannin [ME187]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Wortmannin and for 24h with bFGF

value: Wortmannin

value: 80 nM


title: JM8.N4_bFGF_Purmorphamine [ME192]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Purmorphamine and for 24h with bFGF

value: Purmorphamine


title: JM8.N4_BMP4_PF573228 [ME165]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with PF573228 and for 24h with BMP4


title: JM8.N4_BMP4_Olomoucine [ME152]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Olomoucine and for 24h with BMP4

value: Olomoucine

value: 30 uM


title: JM8.N4_BMP4_PD173074 [ME159]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with PD173074 and for 24h with BMP4

value: mESC


title: JM8.N4_bFGF_PF573228 [ME189]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with PF573228 and for 24h with bFGF


title: JM8.N4_bFGF_PD173074 [ME183]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with PD173074 and for 24h with bFGF


title: JM8.N4_bFGF_SB202190 [ME180]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with SB202190 and for 24h with bFGF

value: 1 uM


title: JM8.N4_bFGF_LDN-193189 [ME178]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with LDN-193189 and for 24h with bFGF


title: JM8.N4_bFGF_DAPT,-gamma-secretase-inhibitor-IX [ME181]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with DAPT, gamma secretase inhibitor IX and for 24h with bFGF

value: DAPT, gamma secretase inhibitor IX


title: JM8.N4_bFGF_Bix01294 [ME185]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Bix01294 and for 24h with bFGF


title: JM8.N4_bFGF_Telomerase-Inhibitor-3 [ME179]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Telomerase Inhibitor 3 and for 24h with bFGF

value: Telomerase Inhibitor 3

value: 2 uM


title: JM8.N4_bFGF_CHIR99021 [ME190]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with CHIR99021 and for 24h with bFGF

value: 3 uM


title: JM8.N4_bFGF_BAPTA-AM [ME191]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with BAPTA-AM and for 24h with bFGF

value: bFGF


title: JM8.N4_Activin-A_Valproic-Acid [ME196]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Valproic Acid and for 24h with Activin A

value: Activin A

value: 20 ng/ml


title: JM8.N4_Activin-A_Wortmannin [ME211]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Wortmannin and for 24h with Activin A


title: JM8.N4_bFGF_BIO [ME171]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with BIO and for 24h with bFGF

value: BIO

value: 6 ng/ml


title: JM8.N4_Retinoic-acid_10058-F4 [ME125]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with 10058-F4 and for 24h with Retinoic acid

value: Retinoic acid

value: 5 uM


title: JM8.N4_Activin-A_Telomerase-Inhibitor-3 [ME203]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Telomerase Inhibitor 3 and for 24h with Activin A


title: JM8.N4_BMP4_Bix01294 [ME161]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Bix01294 and for 24h with BMP4

value: 2 ng/ml


title: JM8.N4_Activin-A_SU5402 [ME206]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with SU5402 and for 24h with Activin A


title: JM8.N4_Activin-A_Olomoucine [ME200]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Olomoucine and for 24h with Activin A


title: JM8.N4_Activin-A_SB202190 [ME204]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with SB202190 and for 24h with Activin A


title: JM8.N4_bFGF_SU5402 [ME182]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with SU5402 and for 24h with bFGF


title: JM8.N4_Activin-A_Purmorphamine [ME216]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Purmorphamine and for 24h with Activin A

value: Purmorphamine


title: JM8.N4_Activin-A_Src-Inhibitor-II [ME201]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Src Inhibitor II and for 24h with Activin A

value: Src Inhibitor II

value: 10 uM


title: JM8.N4_Activin-A_LDN-193189 [ME202]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with LDN-193189 and for 24h with Activin A

value: mESC


title: JM8.N4_bFGF_Src-Inhibitor-II [ME177]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Src Inhibitor II and for 24h with bFGF


title: JM8.N4_Activin-A_Genistein [ME208]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Genistein and for 24h with Activin A

value: Genistein


title: JM8.N4_Activin-A_CHIR99021 [ME214]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with CHIR99021 and for 24h with Activin A


title: JM8.N4_Activin-A_KAAD-Cyclopamine [ME199]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with KAAD-Cyclopamine and for 24h with Activin A

value: KAAD-Cyclopamine


title: JM8.N4_bFGF_Olomoucine [ME176]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Olomoucine and for 24h with bFGF

value: Olomoucine

value: 30 uM


title: JM8.N4_BMP4_BAY-11-7082 [ME145]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with BAY 11-7082 and for 24h with BMP4

value: 20 uM


title: 032216-91_NT_Shc202

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of Shc202 targeting entrez GeneID NT

data_processing:
Sequencing data was demultiplexed, debarcoded, and mapped to the mouse genome GRCm38 (mm10) with the STAR aligner (PMID: 23104886) and counted using ENSEMBL gene annotation.
Gene identifiers were transformed to Entrez GeneIDs.
Assembly: mm10 (GRCm38)
Supplementary files format and content: Raw-counts per known gene using EntrezID gene identifiers

source_name: Epiblast Stem Cell

extract_protocol:
72h after transduction the cells were lysed with Trizol (ThermoFisher, 15596018).
Sample processing and library preparation were performed as described for PLATE-Seq (PMID 28740083).

treatment_protocol: shRNA hairpins targeting to candidate pluripotency regulators were selected and ordered from MISSION shRNA Lentiviral library. All the shRNA lentivirus used for initial screen are produced by Sigma, and tested for high titer. EpiSCs were passaged onto matrigel-coated 96-well plates (Greiner 655090) at a density of 3,000 cells per well, with 100ul CMAF medium supplemented with 10uM Y27632 added in each well. 12 hours after passaging, when cells re-attached to the plate, 50ul of the culturing medium is replaced with 50ul lentivirus supplemented with 8ug/ml Polybrene. 24 hrs after lentivirus transduction, culturing medium is replaced with 100ul CMAF + 2ug/ml Puromycin (Sigma, P7255) for selecting stably integrated cells. Concentration of Puromycin is increased to 4ug/ml by replacing the medium again at 48 h after transduction.

value: EpiSC

tag: shRNA clone

tag: shRNA geneid

value: NT


title: JM8.N4_bFGF_Genistein [ME184]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with Genistein and for 24h with bFGF

value: bFGF


title: 032216-94_NT_Shc202


title: 032216-89_NT_Shc001

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of Shc001 targeting entrez GeneID NT


title: 032216-88_NT_NT

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of NT targeting entrez GeneID NT


title: JM8.N4_Activin-A_PD173074 [ME207]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with PD173074 and for 24h with Activin A

value: Activin A

value: 20 ng/ml


title: 032216-87_20674_TRCN0000416106

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000416106 targeting entrez GeneID 20674


title: 032216-90_NT_Shc002

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of Shc002 targeting entrez GeneID NT


title: 032216-77_NT_Shc002


title: 032216-93_NT_Shc002


title: 032216-78_NT_Shc202


title: 032216-79_20674_TRCN0000420955

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000420955 targeting entrez GeneID 20674


title: 032216-73_NT_Shc002


title: 032216-67_17126_TRCN0000327447

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000327447 targeting entrez GeneID 17126


title: JM8.N4_Activin-A_BAPTA-AM [ME215]

source_name: Mouse ES cells, JM8.N4, perturbed for 30h with BAPTA-AM and for 24h with Activin A

value: BAPTA-AM

value: 4 uM


title: 032216-70_18991_TRCN0000425465

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000425465 targeting entrez GeneID 18991


title: 032216-95_NT_NT


title: 032216-69_217127_TRCN0000366275

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000366275 targeting entrez GeneID 217127


title: 032216-65_106205_TRCN0000322502

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000322502 targeting entrez GeneID 106205


title: 032216-62_18991_TRCN0000431194

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000431194 targeting entrez GeneID 18991


title: 032216-60_NT_Shc001


title: 032216-61_81703_TRCN0000374276

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000374276 targeting entrez GeneID 81703


title: 032216-58_70650_TRCN0000309569

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000309569 targeting entrez GeneID 70650


title: 032216-59_17126_TRCN0000327446

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000327446 targeting entrez GeneID 17126


title: 032216-84_NT_NT


title: 032216-71_20674_TRCN0000424718

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000424718 targeting entrez GeneID 20674


title: 032216-82_NT_Shc202


title: 032216-57_106205_TRCN0000322503

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000322503 targeting entrez GeneID 106205


title: 032216-52_16468_TRCN0000368844

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000368844 targeting entrez GeneID 16468


title: 032216-46_18999_TRCN0000426781

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000426781 targeting entrez GeneID 18999


title: 032216-53_81703_TRCN0000379069

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000379069 targeting entrez GeneID 81703


title: 032216-55_Mock_Mock

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of Mock targeting entrez GeneID Mock

value: Mock


title: 032216-44_16468_TRCN0000362780

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000362780 targeting entrez GeneID 16468


title: 032216-45_81703_TRCN0000374274

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000374274 targeting entrez GeneID 81703


title: 032216-54_18999_TRCN0000430010

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000430010 targeting entrez GeneID 18999


title: 032216-36_16468_TRCN0000362782

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000362782 targeting entrez GeneID 16468


title: 032216-66_70650_TRCN0000309637

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000309637 targeting entrez GeneID 70650


title: 032216-41_Mock_Mock


title: 032216-37_Mock_Mock


title: 032216-38_104383_TRCN0000414131

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000414131 targeting entrez GeneID 104383


title: 032216-27_80292_TRCN0000302898

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000302898 targeting entrez GeneID 80292


title: 032216-28_16468_TRCN0000362710

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000362710 targeting entrez GeneID 16468


title: 032216-31_Mock_Mock


title: 032216-22_Mock_Mock


title: 032216-30_104383_TRCN0000425308

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000425308 targeting entrez GeneID 104383


title: 032216-21_15260_TRCN0000365937

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000365937 targeting entrez GeneID 15260


title: 032216-11_80292_TRCN0000302981

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000302981 targeting entrez GeneID 80292


title: 032216-8_Mock_Mock


title: 032216-7_Mock_Mock


title: 032216-13_15260_TRCN0000365935

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000365935 targeting entrez GeneID 15260


title: 032216-92_NT_NT

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of NT targeting entrez GeneID NT


title: 032216-19_80292_TRCN0000302899

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000302899 targeting entrez GeneID 80292


title: 032216-56_NT_Shc001

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of Shc001 targeting entrez GeneID NT


title: 032216-6_Mock_Mock


title: 032216-5_Mock_Mock


title: 032216-4_Mock_Mock


title: 032216-3_Mock_Mock


title: 040216-94_NT_Shc202

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of Shc202 targeting entrez GeneID NT


title: 040216-89_NT_Shc001


title: 032216-1_Mock_Mock

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of Mock targeting entrez GeneID Mock


title: 032216-2_Mock_Mock


title: 040216-93_NT_Shc002

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of Shc002 targeting entrez GeneID NT


title: 040216-87_NT_NT


title: 040216-81_NT_NT


title: 040216-91_NT_Shc202


title: 040216-90_NT_Shc002


title: 040216-88_NT_NT


title: 040216-68_269023_TRCN0000249823

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000249823 targeting entrez GeneID 269023


title: 040216-71_12396_TRCN0000317317

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000317317 targeting entrez GeneID 12396


title: 040216-64_19009_TRCN0000310937

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000310937 targeting entrez GeneID 19009


title: 040216-76_269023_TRCN0000249822

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000249822 targeting entrez GeneID 269023


title: 040216-72_19009_TRCN0000315998

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000315998 targeting entrez GeneID 19009


title: 040216-61_NT_Shc202


title: 040216-95_NT_NT


title: 040216-49_17869_TRCN0000234924

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000234924 targeting entrez GeneID 17869


title: 040216-55_NT_Shc202


title: 040216-60_66855_TRCN0000241028

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000241028 targeting entrez GeneID 66855


title: 040216-52_66855_TRCN0000241032

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000241032 targeting entrez GeneID 66855


title: 040216-46_11568_TRCN0000295410

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000295410 targeting entrez GeneID 11568


title: 040216-57_17869_TRCN0000234926

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000234926 targeting entrez GeneID 17869


title: 040216-43_NT_Shc202


title: 040216-92_NT_NT


title: 040216-38_11568_TRCN0000295342

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000295342 targeting entrez GeneID 11568


title: 040216-41_17869_TRCN0000234927

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000234927 targeting entrez GeneID 17869


title: 040216-36_332221_TRCN0000239121

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000239121 targeting entrez GeneID 332221


title: 040216-31_NT_Shc002


title: 040216-30_11568_TRCN0000295343

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000295343 targeting entrez GeneID 11568


title: 040216-35_22687_TRCN0000238004

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000238004 targeting entrez GeneID 22687


title: 040216-29_76893_TRCN0000277335

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000277335 targeting entrez GeneID 76893


title: 040216-28_332221_TRCN0000239119

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000239119 targeting entrez GeneID 332221


title: 040216-27_22687_TRCN0000238006

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000238006 targeting entrez GeneID 22687


title: 040216-79_19720_TRCN0000317233

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000317233 targeting entrez GeneID 19720


title: 040216-23_20848_TRCN0000301944

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000301944 targeting entrez GeneID 20848


title: 040216-22_NT_Shc002

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of Shc002 targeting entrez GeneID NT


title: 040216-21_NT_Shc002


title: 040216-86_11820_TRCN0000301472

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000301472 targeting entrez GeneID 11820


title: 040216-15_11820_TRCN0000301544

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000301544 targeting entrez GeneID 11820


title: 040216-14_NT_Shc001

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of Shc001 targeting entrez GeneID NT


title: 040216-13_100515_TRCN0000218746

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000218746 targeting entrez GeneID 100515


title: 040216-8_Mock_Mock

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of Mock targeting entrez GeneID Mock


title: 040216-5_Mock_Mock


title: 040216-4_Mock_Mock


title: 040216-7_Mock_Mock


title: 040216-2_Mock_Mock


title: 040216-1_Mock_Mock


title: 040216-44_66855_TRCN0000241031

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000241031 targeting entrez GeneID 66855


title: 040216-3_Mock_Mock


title: 031916-94_NT_Shc202

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of Shc202 targeting entrez GeneID NT


title: 031916-95_NT_NT

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of NT targeting entrez GeneID NT


title: 031916-93_NT_Shc002


title: 031916-91_NT_Shc202


title: 031916-90_NT_Shc002


title: 031916-86_231986_TRCN0000127433

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000127433 targeting entrez GeneID 231986


title: 031916-92_NT_NT


title: 031916-89_NT_Shc001


title: 031916-85_19183_TRCN0000120276

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000120276 targeting entrez GeneID 19183


title: 031916-84_21769_TRCN0000106378

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000106378 targeting entrez GeneID 21769


title: 040216-20_332221_TRCN0000239120

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000239120 targeting entrez GeneID 332221


title: 031916-76_29871_TRCN0000097999

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000097999 targeting entrez GeneID 29871


title: 031916-79_76893_TRCN0000215319

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000215319 targeting entrez GeneID 76893


title: 031916-82_22764_TRCN0000095621

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000095621 targeting entrez GeneID 22764


title: 031916-72_NT_NT


title: 031916-81_NT_NT


title: 040216-6_Mock_Mock


title: 031916-74_22764_TRCN0000095619

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000095619 targeting entrez GeneID 22764


title: 031916-70_NT_NT


title: 031916-77_19183_TRCN0000120274

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000120274 targeting entrez GeneID 19183


title: 031916-69_19183_TRCN0000120272

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000120272 targeting entrez GeneID 19183


title: 031916-65_17126_TRCN0000089336

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000089336 targeting entrez GeneID 17126


title: 031916-71_67302_TRCN0000216806

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000216806 targeting entrez GeneID 67302


title: 031916-68_NT_Shc202


title: 031916-67_NT_Shc202


title: 040216-19_NT_Shc001

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of Shc001 targeting entrez GeneID NT


title: 031916-63_67302_TRCN0000217068

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000217068 targeting entrez GeneID 67302


title: 031916-66_12396_TRCN0000095617

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000095617 targeting entrez GeneID 12396


title: 031916-52_99377_TRCN0000097820

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000097820 targeting entrez GeneID 99377


title: 031916-57_15191_TRCN0000089225

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000089225 targeting entrez GeneID 15191


title: 031916-59_211586_TRCN0000095781

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000095781 targeting entrez GeneID 211586


title: 031916-51_211586_TRCN0000095780

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000095780 targeting entrez GeneID 211586


title: 031916-50_71041_TRCN0000095301

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000095301 targeting entrez GeneID 71041


title: 031916-55_67302_TRCN0000217812

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000217812 targeting entrez GeneID 67302


title: 031916-49_15191_TRCN0000089224

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000089224 targeting entrez GeneID 15191


title: 031916-53_NT_Shc002

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of Shc002 targeting entrez GeneID NT


title: 031916-45_26940_TRCN0000113957

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000113957 targeting entrez GeneID 26940


title: 031916-44_14025_TRCN0000096552

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000096552 targeting entrez GeneID 14025


title: 031916-41_15191_TRCN0000089223

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000089223 targeting entrez GeneID 15191


title: 031916-43_22773_TRCN0000095727

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000095727 targeting entrez GeneID 22773


title: 031916-46_70650_TRCN0000123779

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000123779 targeting entrez GeneID 70650


title: 031916-36_14025_TRCN0000096549

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000096549 targeting entrez GeneID 14025


title: 031916-42_71041_TRCN0000095300

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000095300 targeting entrez GeneID 71041


title: 031916-37_26940_TRCN0000113956

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000113956 targeting entrez GeneID 26940


title: 031916-34_12705_TRCN0000095238

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000095238 targeting entrez GeneID 12705


title: 031916-27_22773_TRCN0000095725

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000095725 targeting entrez GeneID 22773


title: 031916-23_269639_TRCN0000173169

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000173169 targeting entrez GeneID 269639


title: 031916-26_12705_TRCN0000095236

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000095236 targeting entrez GeneID 12705


title: 031916-60_99377_TRCN0000097821

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000097821 targeting entrez GeneID 99377


title: 031916-22_NT_Shc002


title: 031916-16_106205_TRCN0000217650

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000217650 targeting entrez GeneID 106205


title: 031916-19_22773_TRCN0000095724

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000095724 targeting entrez GeneID 22773


title: 031916-17_NT_Shc001

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of Shc001 targeting entrez GeneID NT


title: 031916-15_22687_TRCN0000175245

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000175245 targeting entrez GeneID 22687


title: 031916-14_68479_TRCN0000123415

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000123415 targeting entrez GeneID 68479


title: 031916-13_21769_TRCN0000106379

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000106379 targeting entrez GeneID 21769


title: 031916-7_Mock_Mock

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of Mock targeting entrez GeneID Mock


title: 031916-6_Mock_Mock


title: 031916-11_22764_TRCN0000095623

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000095623 targeting entrez GeneID 22764


title: 031916-3_Mock_Mock


title: 031916-5_Mock_Mock


title: 031916-1_Mock_Mock


title: 031716-95_NT_NT

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of NT targeting entrez GeneID NT


title: 031716-94_NT_Shc202

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of Shc202 targeting entrez GeneID NT


title: 031716-92_NT_NT


title: 031716-90_NT_Shc002

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of Shc002 targeting entrez GeneID NT


title: 031716-91_NT_Shc202


title: 031716-89_NT_Shc001


title: 031716-88_NT_NT


title: 031716-82_16918_TRCN0000042709

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000042709 targeting entrez GeneID 16918


title: 031916-35_22773_TRCN0000095726

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000095726 targeting entrez GeneID 22773


title: 031716-86_NT_NT


title: 031716-81_NT_NT


title: 031716-80_21780_TRCN0000086064

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000086064 targeting entrez GeneID 21780


title: 031716-77_NT_Shc202


title: 031716-74_213753_TRCN0000040851

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000040851 targeting entrez GeneID 213753


title: 031716-69_71950_TRCN0000075336

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000075336 targeting entrez GeneID 71950


title: 031916-8_NT_Shc001


title: 031716-62_52502_TRCN0000077371

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000077371 targeting entrez GeneID 52502


title: 031716-66_213753_TRCN0000040850

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000040850 targeting entrez GeneID 213753


title: 031916-4_Mock_Mock


title: 031916-2_Mock_Mock


title: 031716-61_71950_TRCN0000075335

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000075335 targeting entrez GeneID 71950


title: 031716-58_213753_TRCN0000040849

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000040849 targeting entrez GeneID 213753


title: 031716-57_18514_TRCN0000012577

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000012577 targeting entrez GeneID 18514


title: 031716-53_71950_TRCN0000075334

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000075334 targeting entrez GeneID 71950


title: 031716-50_19720_TRCN0000040540

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000040540 targeting entrez GeneID 19720


title: 031716-93_NT_Shc002


title: 031716-42_217127_TRCN0000039242

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000039242 targeting entrez GeneID 217127


title: 031716-39_60611_TRCN0000084291

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000084291 targeting entrez GeneID 60611


title: 031716-40_104383_TRCN0000085521

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000085521 targeting entrez GeneID 104383


title: 031716-47_60611_TRCN0000084292

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000084292 targeting entrez GeneID 60611


title: 031716-46_52502_TRCN0000077369

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000077369 targeting entrez GeneID 52502


title: 031716-38_NT_Shc002

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of Shc002 targeting entrez GeneID NT


title: 031716-30_14009_TRCN0000075477

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000075477 targeting entrez GeneID 14009


title: 031716-36_56449_TRCN0000070437

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000070437 targeting entrez GeneID 56449


title: 031716-29_20848_TRCN0000071456

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000071456 targeting entrez GeneID 20848


title: 031716-28_56449_TRCN0000070436

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000070436 targeting entrez GeneID 56449


title: 031716-27_11820_TRCN0000054874

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000054874 targeting entrez GeneID 11820


title: 031716-13_20848_TRCN0000071453

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000071453 targeting entrez GeneID 20848


title: 031716-22_NT_Shc001

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of Shc001 targeting entrez GeneID NT


title: 031716-35_NT_Shc001


title: 031716-19_17869_TRCN0000054853

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000054853 targeting entrez GeneID 17869


title: 031716-54_NT_Shc202

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of Shc202 targeting entrez GeneID NT


title: 031716-21_20848_TRCN0000071454

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000071454 targeting entrez GeneID 20848


title: 031716-6_Mock_Mock

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of Mock targeting entrez GeneID Mock


title: 031716-49_NT_Shc002


title: 031716-3_Mock_Mock


title: 031716-2_Mock_Mock


title: 031716-4_Mock_Mock


title: 031716-14_14009_TRCN0000075473

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000075473 targeting entrez GeneID 14009


title: 031716-5_Mock_Mock


title: 031716-7_Mock_Mock


title: 031716-8_Mock_Mock


title: 031716-1_Mock_Mock


title: 031716-41_18999_TRCN0000009612

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000009612 targeting entrez GeneID 18999


title: 022316-92_NT_NT

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of NT targeting entrez GeneID NT


title: 022316-95_NT_NT


title: 022316-91_NT_NT


title: 022316-94_NT_Shc202


title: 022316-90_NT_NT


title: 022316-88_NT_NT


title: 022316-89_NT_NT


title: 022316-78_227656_TRCN0000375918

description: Mouse EpiSC-5 cells, 72h after lentivirus-mediated transduccion of TRCN0000375918 targeting entrez GeneID 227656


title: 022316-80_NT_NT